LOCUS       DARBEC010000001       148258 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.001, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000001 DARBEC010000000
VERSION     DARBEC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 148258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 148258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..148258
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.001"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(12..1535)
                     /locus_tag="SCL80_000001"
     CDS             complement(12..1535)
                     /locus_tag="SCL80_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1105904.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            1787..2968
                     /locus_tag="SCL80_000002"
     CDS             1787..2968
                     /locus_tag="SCL80_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1105905.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCL80_000003"
     CDS             complement(3028..4656)
                     /gene="treC"
                     /locus_tag="SCL80_000003"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEG1105906.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(5053..7764)
                     /locus_tag="SCL80_000004"
     CDS             complement(5053..7764)
                     /locus_tag="SCL80_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1105907.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            complement(7774..8475)
                     /locus_tag="SCL80_000005"
     CDS             complement(7774..8475)
                     /locus_tag="SCL80_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1105908.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            8661..9260
                     /locus_tag="SCL80_000006"
     CDS             8661..9260
                     /locus_tag="SCL80_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1105909.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            9341..10354
                     /locus_tag="SCL80_000007"
     CDS             9341..10354
                     /locus_tag="SCL80_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1105910.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            10462..11379
                     /gene="rbsK"
                     /locus_tag="SCL80_000008"
     CDS             10462..11379
                     /gene="rbsK"
                     /locus_tag="SCL80_000008"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEG1105911.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            11407..11802
                     /gene="rbsD"
                     /locus_tag="SCL80_000009"
     CDS             11407..11802
                     /gene="rbsD"
                     /locus_tag="SCL80_000009"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEG1105912.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            11802..13175
                     /locus_tag="SCL80_000010"
     CDS             11802..13175
                     /locus_tag="SCL80_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1105913.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            13532..15676
                     /locus_tag="SCL80_000011"
     CDS             13532..15676
                     /locus_tag="SCL80_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1105914.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(15728..16768)
                     /locus_tag="SCL80_000012"
     CDS             complement(15728..16768)
                     /locus_tag="SCL80_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1105915.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMGNGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(17045..18847)
                     /locus_tag="SCL80_000013"
     CDS             complement(17045..18847)
                     /locus_tag="SCL80_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1105916.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            complement(18861..19613)
                     /locus_tag="SCL80_000014"
     CDS             complement(18861..19613)
                     /locus_tag="SCL80_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1105917.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            complement(19622..19984)
                     /locus_tag="SCL80_000015"
     CDS             complement(19622..19984)
                     /locus_tag="SCL80_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1105918.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            complement(20160..21032)
                     /locus_tag="SCL80_000016"
     CDS             complement(20160..21032)
                     /locus_tag="SCL80_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1105919.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            complement(21058..21570)
                     /locus_tag="SCL80_000017"
     CDS             complement(21058..21570)
                     /locus_tag="SCL80_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEG1105920.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            complement(21708..22499)
                     /locus_tag="SCL80_000018"
     CDS             complement(21708..22499)
                     /locus_tag="SCL80_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEG1105921.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            complement(22554..23162)
                     /locus_tag="SCL80_000019"
     CDS             complement(22554..23162)
                     /locus_tag="SCL80_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEG1105922.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            complement(23155..23472)
                     /locus_tag="SCL80_000020"
     CDS             complement(23155..23472)
                     /locus_tag="SCL80_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1105923.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            complement(23628..24356)
                     /locus_tag="SCL80_000021"
     CDS             complement(23628..24356)
                     /locus_tag="SCL80_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEG1105924.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            24515..25396
                     /locus_tag="SCL80_000022"
     CDS             24515..25396
                     /locus_tag="SCL80_000022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1105925.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            complement(25633..26676)
                     /locus_tag="SCL80_000023"
     CDS             complement(25633..26676)
                     /locus_tag="SCL80_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1105926.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIG
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(26679..27347)
                     /locus_tag="SCL80_000024"
     CDS             complement(26679..27347)
                     /locus_tag="SCL80_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1105927.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            27489..29825
                     /locus_tag="SCL80_000025"
     CDS             27489..29825
                     /locus_tag="SCL80_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1105928.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTDHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            complement(29940..30251)
                     /locus_tag="SCL80_000026"
     CDS             complement(29940..30251)
                     /locus_tag="SCL80_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1105929.1"
                     /translation="MKKMKESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            30432..31907
                     /locus_tag="SCL80_000027"
     CDS             30432..31907
                     /locus_tag="SCL80_000027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEG1105930.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGMSAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            31954..32538
                     /locus_tag="SCL80_000028"
     CDS             31954..32538
                     /locus_tag="SCL80_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEG1105931.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFGIVNEHSKRWQGD"
     gene            32540..32959
                     /locus_tag="SCL80_000029"
     CDS             32540..32959
                     /locus_tag="SCL80_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEG1105932.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKTL"
     gene            complement(33240..33524)
                     /locus_tag="SCL80_000030"
     CDS             complement(33240..33524)
                     /locus_tag="SCL80_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEG1105933.1"
                     /translation="MREIYSFESKIYASEVDKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNMPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(33876..34586)
                     /locus_tag="SCL80_000031"
     CDS             complement(33876..34586)
                     /locus_tag="SCL80_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEG1105934.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
     gene            complement(34583..36055)
                     /locus_tag="SCL80_000032"
     CDS             complement(34583..36055)
                     /locus_tag="SCL80_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1105935.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            36193..36666
                     /locus_tag="SCL80_000033"
     CDS             36193..36666
                     /locus_tag="SCL80_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1105936.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            complement(36819..37370)
                     /locus_tag="SCL80_000034"
     CDS             complement(36819..37370)
                     /locus_tag="SCL80_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1105937.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            37475..38005
                     /locus_tag="SCL80_000035"
     CDS             37475..38005
                     /locus_tag="SCL80_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEG1105938.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            complement(38488..38931)
                     /locus_tag="SCL80_000036"
     CDS             complement(38488..38931)
                     /locus_tag="SCL80_000036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1105939.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(38947..40107)
                     /locus_tag="SCL80_000037"
     CDS             complement(38947..40107)
                     /locus_tag="SCL80_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1105940.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKKQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            40614..42314
                     /locus_tag="SCL80_000038"
     CDS             40614..42314
                     /locus_tag="SCL80_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1105941.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQKKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            complement(42416..43249)
                     /locus_tag="SCL80_000039"
     CDS             complement(42416..43249)
                     /locus_tag="SCL80_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEG1105942.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCQVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQSQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(43553..45718)
                     /locus_tag="SCL80_000040"
     CDS             complement(43553..45718)
                     /locus_tag="SCL80_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1105943.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            45978..48272
                     /locus_tag="SCL80_000041"
     CDS             45978..48272
                     /locus_tag="SCL80_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1105944.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGFPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            48265..48930
                     /gene="pgmB"
                     /locus_tag="SCL80_000042"
     CDS             48265..48930
                     /gene="pgmB"
                     /locus_tag="SCL80_000042"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1105945.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKCH
                     DHG"
     gene            48923..49930
                     /locus_tag="SCL80_000043"
     CDS             48923..49930
                     /locus_tag="SCL80_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1105946.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            49952..50971
                     /locus_tag="SCL80_000044"
     CDS             49952..50971
                     /locus_tag="SCL80_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1105947.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDPVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCL80_000045"
     CDS             complement(51252..52619)
                     /gene="nhaC"
                     /locus_tag="SCL80_000045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1105948.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFVFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(52729..54150)
                     /locus_tag="SCL80_000046"
     CDS             complement(52729..54150)
                     /locus_tag="SCL80_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1105949.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCL80_000047"
     CDS             complement(54393..56270)
                     /gene="tdc"
                     /locus_tag="SCL80_000047"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1105950.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(56561..57817)
                     /locus_tag="SCL80_000048"
     CDS             complement(56561..57817)
                     /locus_tag="SCL80_000048"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEG1105951.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEVEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(57929..58134)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            58397..59698
                     /locus_tag="SCL80_000049"
     CDS             58397..59698
                     /locus_tag="SCL80_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1105952.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(59767..60366)
                     /locus_tag="SCL80_000050"
     CDS             complement(59767..60366)
                     /locus_tag="SCL80_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1105953.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            60620..61501
                     /locus_tag="SCL80_000051"
     CDS             60620..61501
                     /locus_tag="SCL80_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1105954.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTVVEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            61514..63124
                     /locus_tag="SCL80_000052"
     CDS             61514..63124
                     /locus_tag="SCL80_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEG1105955.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRMGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(63201..63860)
                     /locus_tag="SCL80_000053"
     CDS             complement(63201..63860)
                     /locus_tag="SCL80_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1105956.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(64143..64808)
                     /locus_tag="SCL80_000054"
     CDS             complement(64143..64808)
                     /locus_tag="SCL80_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEG1105957.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCL80_000055"
     CDS             complement(64955..65560)
                     /gene="dhaL"
                     /locus_tag="SCL80_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEG1105958.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCL80_000056"
     CDS             complement(65588..66598)
                     /gene="dhaK"
                     /locus_tag="SCL80_000056"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEG1105959.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCL80_000057"
     CDS             complement(66573..67019)
                     /gene="dhaM"
                     /locus_tag="SCL80_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEG1105960.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
     gene            67122..67442
                     /locus_tag="SCL80_000058"
     CDS             67122..67442
                     /locus_tag="SCL80_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEG1105961.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            complement(67554..68423)
                     /locus_tag="SCL80_000059"
     CDS             complement(67554..68423)
                     /locus_tag="SCL80_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1105962.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(68519..69822)
                     /locus_tag="SCL80_000060"
                     /pseudo
     CDS             complement(68519..69822)
                     /locus_tag="SCL80_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(69819..70328)
                     /locus_tag="SCL80_000061"
     CDS             complement(69819..70328)
                     /locus_tag="SCL80_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1105963.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYEDEKLKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            complement(70503..72638)
                     /locus_tag="SCL80_000062"
     CDS             complement(70503..72638)
                     /locus_tag="SCL80_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1105964.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(72721..74376)
                     /locus_tag="SCL80_000063"
     CDS             complement(72721..74376)
                     /locus_tag="SCL80_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1105965.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(74378..74692)
                     /locus_tag="SCL80_000064"
     CDS             complement(74378..74692)
                     /locus_tag="SCL80_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1105966.1"
                     /translation="MNLLFQSIKSITVPELKEKLLENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            74862..75122
                     /locus_tag="SCL80_000065"
     CDS             74862..75122
                     /locus_tag="SCL80_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEG1105967.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            75136..75450
                     /locus_tag="SCL80_000066"
     CDS             75136..75450
                     /locus_tag="SCL80_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEG1105968.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(76180..76734)
                     /locus_tag="SCL80_000067"
     CDS             complement(76180..76734)
                     /locus_tag="SCL80_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEG1105969.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(76860..78788)
                     /locus_tag="SCL80_000068"
     CDS             complement(76860..78788)
                     /locus_tag="SCL80_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1105970.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(79094..79810)
                     /locus_tag="SCL80_000069"
     CDS             complement(79094..79810)
                     /locus_tag="SCL80_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1105971.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCL80_000070"
     CDS             complement(79811..81634)
                     /gene="glpO"
                     /locus_tag="SCL80_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEG1105972.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKYENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCL80_000071"
     CDS             complement(81648..83144)
                     /gene="glpK"
                     /locus_tag="SCL80_000071"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEG1105973.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(83280..84740)
                     /locus_tag="SCL80_000072"
     CDS             complement(83280..84740)
                     /locus_tag="SCL80_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1105974.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(84730..86025)
                     /locus_tag="SCL80_000073"
     CDS             complement(84730..86025)
                     /locus_tag="SCL80_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1105975.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVEEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(86357..87034)
                     /locus_tag="SCL80_000074"
     CDS             complement(86357..87034)
                     /locus_tag="SCL80_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEG1105976.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(87064..88155)
                     /locus_tag="SCL80_000075"
     CDS             complement(87064..88155)
                     /locus_tag="SCL80_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEG1105977.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(88256..88690)
                     /locus_tag="SCL80_000076"
     CDS             complement(88256..88690)
                     /locus_tag="SCL80_000076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1105978.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCL80_000077"
     CDS             complement(89274..90731)
                     /gene="ascB"
                     /locus_tag="SCL80_000077"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1105979.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENW
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIKDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(90728..92623)
                     /locus_tag="SCL80_000078"
     CDS             complement(90728..92623)
                     /locus_tag="SCL80_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1105980.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(92681..94456)
                     /locus_tag="SCL80_000079"
     CDS             complement(92681..94456)
                     /locus_tag="SCL80_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1105981.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYFVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESWEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(94506..96002)
                     /locus_tag="SCL80_000080"
     CDS             complement(94506..96002)
                     /locus_tag="SCL80_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1105982.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     VIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRTWKEEDCAPVMLDGDEQCLQANTADYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(96196..97818)
                     /locus_tag="SCL80_000081"
     CDS             complement(96196..97818)
                     /locus_tag="SCL80_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1105983.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(98267..99580)
                     /locus_tag="SCL80_000082"
     CDS             complement(98267..99580)
                     /locus_tag="SCL80_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1105984.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCVLMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGIAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLFGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFVGFMWILNVK"
     gene            complement(99825..100049)
                     /locus_tag="SCL80_000083"
     CDS             complement(99825..100049)
                     /locus_tag="SCL80_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1105985.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(100069..100491)
                     /locus_tag="SCL80_000084"
     CDS             complement(100069..100491)
                     /locus_tag="SCL80_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEG1105986.1"
                     /translation="MESVYRQERKSNRKKGQMMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGILTFNFSDPEGNYFVIGKAESSD"
     gene            complement(100635..101345)
                     /locus_tag="SCL80_000085"
     CDS             complement(100635..101345)
                     /locus_tag="SCL80_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1105987.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGGESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(101363..101860)
                     /locus_tag="SCL80_000086"
     CDS             complement(101363..101860)
                     /locus_tag="SCL80_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1105988.1"
                     /translation="MTPSDVDLLQQISIETFTDTFGKDNSPEHLADYLTQAYALEKLT
                     EELKDKETDFFLVSIDQEPAGYLKLNTGTSQSEEMADHFLEIERIYVRKKFKHQGIGT
                     FLLDTAIEKAKEKQKTAIWLGVWEHNDVALAFYRKKGFIKYSEHVFYMGDDPQTDYLF
                     IKQLF"
     gene            complement(101897..102349)
                     /locus_tag="SCL80_000087"
     CDS             complement(101897..102349)
                     /locus_tag="SCL80_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1105989.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(102484..103089)
                     /locus_tag="SCL80_000088"
     CDS             complement(102484..103089)
                     /locus_tag="SCL80_000088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1105990.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(103539..104159)
                     /locus_tag="SCL80_000089"
     CDS             complement(103539..104159)
                     /locus_tag="SCL80_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1105991.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLCQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(104161..105030)
                     /locus_tag="SCL80_000090"
     CDS             complement(104161..105030)
                     /locus_tag="SCL80_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEG1105992.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(105053..105460)
                     /locus_tag="SCL80_000091"
     CDS             complement(105053..105460)
                     /locus_tag="SCL80_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1105993.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(105479..106768)
                     /locus_tag="SCL80_000092"
     CDS             complement(105479..106768)
                     /locus_tag="SCL80_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1105994.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(106795..107097)
                     /locus_tag="SCL80_000093"
     CDS             complement(106795..107097)
                     /locus_tag="SCL80_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1105995.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(107122..109215)
                     /locus_tag="SCL80_000094"
     CDS             complement(107122..109215)
                     /locus_tag="SCL80_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1105996.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKVFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLVEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(109704..110468)
                     /locus_tag="SCL80_000095"
     CDS             complement(109704..110468)
                     /locus_tag="SCL80_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEG1105997.1"
                     /translation="MKKINYLAIILLVGLCLFALPTQGFAVAGGHGGGTSSGRSSGRG
                     SFGGRSYGGSYFGSRSYGRDSYGYYGPGTGPSSYEQTGFLALAVLSVGGVSAFSIVKN
                     KRQKKRLKAEILSRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFA
                     EHQKVLQKNKADGVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLI
                     SGSKTQRQYFSQIWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCL80_000096"
     CDS             complement(110579..111448)
                     /gene="mdcB"
                     /locus_tag="SCL80_000096"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase MdcB"
                     /protein_id="HEG1105998.1"
                     /translation="MINDERKKAVTQQLAALVEEALIAEVTLSPKPGLVDALDAGAHS
                     DMDYALFLKSAKTLTPFFEEMAQIAWHHAIDQELRERIAEIGRKAEKAMLIATNGINT
                     HKGAIWAMGLLISVIAQKISMEQPFSLSKILDDVSKLAAFPDTKYRHKKTTHGHRVKQ
                     KYGVNGAYEEAILGYPHIRLAIQAAKELDNDSYVQKQMHMLLVLMSSLDDTCVLHRSD
                     QKTLVEMQKLAHAASQSALPNRPFTALLHFCQENQISPGGSADLLSASLFMLKIEELW
                     TADTYICPMLLND"
     gene            complement(111473..112549)
                     /locus_tag="SCL80_000097"
     CDS             complement(111473..112549)
                     /locus_tag="SCL80_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HEG1105999.1"
                     /translation="MFENKKIKINVSDFQKVSAVTAVMMQTILSFALANSHSATNTAF
                     LGTFYVIVKYTAPMFIFAIVYNMVKTSQHLSYWEFLKEKFFELVVPYLLWTTAYLWLF
                     PAVQQQTPYTDTASFLLKYVTGDGAPHLWYTVMMLQIQLFMPFFVWLGYKVFANKKYV
                     WPVLIGSTILYVAWYLFYQTQVLTGPYHDSWYLLDRFVASFMIYGVAAFVYHEKVYQY
                     LDRVRYLFLPVALVIAFFSVRSLLAHPGDLSFANAPYLNTIQSLYSLVIIFAVFIGAS
                     KMIVNDSPKLPLFKWLSVYAYRTYLANVFVFQVLLLLFKDSWLQLPSGVMILVAYLMT
                     ASCAFALSWLLHIIWVAIKKGFSK"
     gene            complement(112573..113166)
                     /locus_tag="SCL80_000098"
     CDS             complement(112573..113166)
                     /locus_tag="SCL80_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase holo-ACP synthase"
                     /protein_id="HEG1106000.1"
                     /translation="MIQAHDIVLFDKHEIEGFTLPEWVVLTEKMFGTVRRASTDHAEI
                     LPIGLRGPARNQRWSTEISKHAVKQVIHPWDIIEQESYRQEKIMDYPVYQQFHAARNI
                     LSGCKWGIGGSLGFELSTGIPAVKETSDFDLLLYANSPIELPIQVIQSHPAFFEQFDT
                     QVVTRQGGFSLKEYLREPEKKILLKTVEGPKLTKELW"
     gene            complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCL80_000099"
     CDS             complement(113170..114846)
                     /gene="mdcD"
                     /locus_tag="SCL80_000099"
                     /EC_number="4.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin-independent malonate decarboxylase
                     subunit beta"
                     /protein_id="HEG1106001.1"
                     /translation="MKNSFVELNARERVNALLDNGRELIDPFDQIIAPSLPAQGIVPE
                     SDDGIVVSRGTINGKQAVVIAMEGKFQGGGIGEVSGAKIVAALEHVLEENKKGNEIFP
                     VIVLDTGGVRLQEANYGLLSISEIGNVIIALKQYVPVIGLIPGRVGSFGGMSITSALM
                     SYLIATKKARVGLNGPEVIEQEAGVREFDSSDKDLIWNTLGSRQRVESKIIDELVTDS
                     VEAIKAAVIAAMDERKDTHRSESSAFYLSLLDSLDLSKPMAIDDYNEAIANHQAKSID
                     LPEVAEGNEPAAASVGYDWFAALTGMKNPTSSISTVYYGKSSKFIEEAIVTSIVPDPK
                     NPMYRVRNGEVGLVEGFRMGQILDHVYEEDKDKKVKRPIIVVIDVPSQAYGYNEELIG
                     IHVALANSAAAYAKLRQAGHPVIGLIAGNAISGAFLAHGLQSSRLIALNNKEITVQAM
                     SKESAARVTKRSVAEIEAAAEKVPAIAYDINNYTKLGAIYHFLEDITDQAASEQNVSK
                     VIEAINEAIQDVRNTNDTMLTNRYTNETAKTFGRVETNKVRAKMDEEWDA"
     gene            complement(114836..115135)
                     /locus_tag="SCL80_000100"
     CDS             complement(114836..115135)
                     /locus_tag="SCL80_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit delta"
                     /protein_id="HEG1106002.1"
                     /translation="MEQLAYKYPTKQPINKKIHVGVVGSGDLEILMFPTEKTESTVKI
                     CTGSDGFGEVWNNVLTRFFDRYPISADIVINDFGATPGVVYLRLTQAMEELSDEK"
     gene            complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCL80_000101"
     CDS             complement(115237..116883)
                     /gene="mdcA"
                     /locus_tag="SCL80_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HEG1106003.1"
                     /translation="MEKPNRNWASKREKKAQRLEKISNLLNGKYVETEKIVEVMEKLI
                     APGDKVVLEGDNQKQASFLSESLEKVDAEKVNDLHLIMSSISRPEHLNIFEKGIASKI
                     DFSYAGAQSLRVAQMIEDGTMKLGDIHTYLELYGRLFIDLIPNVVLVAADKADKDGNL
                     YTGFNTEETPTIIEAAAFKDGIVIVQVNEVVDSLPRVDIPGDWVDVVVKADKPYQLEA
                     LFTRDPQNITELQILMAMMAIKGIYAEHGVQSLNHGIGFNTAAIELLLPTYGESLGLK
                     GKIAKNWVLNPHPTMIPAIESGWVESIHSFGGEVGMEKYIAARPDVFFVGKDGTMRSN
                     RTLSQAAGQYAIDMFIGSTLQLDYEGNSSTVTKGRLSGFGGAPNMGHNPGGRRHSTPA
                     WQSLRDNDDPLGKGQKLVVQMVETYGSNKKPVFVEELDALAVQKQAGLANAPVMIYSE
                     DTTHIVTEEGIAYLYKAKTKEERQAAIAAIGGVTPLGMTSTKESLDSLRAAGIVKLPE
                     DLGIKRSEAKRSLLAAQTIDDLIEWSDGLYEPPMKFRTWS"
     gene            complement(116890..117801)
                     /locus_tag="SCL80_000102"
     CDS             complement(116890..117801)
                     /locus_tag="SCL80_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1106004.1"
                     /translation="MSTGFLFPGQGSQKLGMLADIPEMYMERLENVTGYRLVDKEENY
                     QDTVFIQLALLTKAVFYLDEMEKNGIQPDIAAGHSIGAFCAAVACRSLRFENAAELVY
                     HRAVLMKEAYPSGYAMGVVVGVTRSEAEEIVAKTFDPDHPVYLSNENCPMQHTLSGSI
                     EGLSVTLAEAKRHQAQTAKLLKVPVPSHCLLMNDTVKKFKPYMQSVHLSDARCLYIKN
                     TDGRATQDAEEIRKDLLENLAFPVQWNQMMDLAKELGMDLTIEFPPGNTLTKLIHTKF
                     GVDKGIRTINLDQHGIDDATFLYQKWR"
     gene            117921..118778
                     /locus_tag="SCL80_000103"
     CDS             117921..118778
                     /locus_tag="SCL80_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1106005.1"
                     /translation="MKLLHLQYFKKVFETKNVTQAAKELFISQPALSRAIKHLENELG
                     VPLFYHSGRNIETTVYAEEFYPYAVKTLDTLEEGIKLLSSVNEKIVTSVILYLEVASV
                     SIPNLVRIFMEKHPDIQLTIMQHHLPDDITDPVLYITSEMKPGMTNIPIMKEPVYVAI
                     PKKHPLAKKEHLQLEDIQRTPILMLSKSNAFRHTLDAALEAKSIELTISSTTDDPATL
                     RSIVKQGLAVSFFPKVSWSYDKNDPFVLREIKDLPLTRTIYLSSSFTEDHPLIKMIAK
                     TMREFYLGK"
     gene            complement(118965..119327)
                     /locus_tag="SCL80_000104"
     CDS             complement(118965..119327)
                     /locus_tag="SCL80_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HEG1106006.1"
                     /translation="MLTKKELIDHINETYNLSPEYIFKKFPDYCVFRHQKSKKWFGVL
                     MSVPEEKIYDDASDHRIINILDLKVDPELGGILRGKEGYFPAYHMNKEHWISIDLRKI
                     EKLDELADLIDGSFKSTQ"
     gene            119673..121112
                     /locus_tag="SCL80_000105"
     CDS             119673..121112
                     /locus_tag="SCL80_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106007.1"
                     /translation="MYSLMKKIITEKDIRRHVSLVEQLLNHTKITVNELAEIIGTTER
                     TIFSDLQSIRSQLPEGWDIISDQAGISLQNQQNLLTNDLWEIFFKQSVSVELLKNLLF
                     TKKVAVPDFLADHGLSYGTLKRHVTKINQRLASYDLQIDLTKYTACILGKERAIRTFY
                     HRLLIPFTHNNYFFEDYSIHESHYFQFLRNLSQTELAVETEEIFGTCWFFINTIRIKA
                     NCRLDSSIHTNSTLSSLYDSALKKLYLTEGIYLKDTELSFASFCFLESWNYNNNYGQE
                     TARCLHHSPFLEVLETFVEELASELSLDQLKKTSLTDNLALLILKYHESPILIEALDR
                     QYHRFLETYDQQYAHLYQQKENLLNDLKKEIEINEPNYFLQLLSLLIQQTIFSIKPNL
                     FNVYFIFQGEPSWKAFLQQELKDYLGKRVCFLPIELTDLSTISFEKTDILISNFPLDS
                     LDIPIVYISSIPTKNELNRLTELTFKTFL"
     gene            complement(121119..123671)
                     /locus_tag="SCL80_000106"
     CDS             complement(121119..123671)
                     /locus_tag="SCL80_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HEG1106008.1"
                     /translation="MNWYRLTVEDVLKNYQTSTEGLSQEERQKRLTDQGYNEIKARQQ
                     TKRWKKIAKHFTDLLMIVLIIASFLKFASSEYIEGSIILFVVIVNGLVSYWQERKAEE
                     SLNGLKQLMGQEAIVLSNGMQEKIPAKELVKGDVIVLRAGDVVPADIRLIEAHDLLVE
                     ESILTGESEASEKSHAVLTEEESIGDQKNMAFSGTLVQSGSAVGVVVETGNTTEIGKI
                     NQALQSVEQQTTPLIKKINRLNKQIFQGILCLSLFLVIFTTFRYGMDWHILLSATIAL
                     VVSMVPEGLPAVLTMILSVGVHEMAKEKAIIKGLPSVETLGSMTVICSDKTGTLTKNE
                     MTVVDVAAKEEACVLSIMKNCQELKTKEEQKTENLSGNPTEVALIRHTETANLPLKKI
                     EAKIPFSSEYKYMATMHAEEKGAVIYVKGAPEVLFAKSTLSSAEQEAWSQTAAEFARK
                     GQRVLGFAYKKVDSKQELTHETLTQLTFAGIAGLIDPPKESAVKAVKECQQAGISVKM
                     ITGDHKDTAKAIAEQIGLKHTAKVLEGIDLDLMSDEELIQQVPIVDVFARTTPEHKLR
                     IVKALQKNGEIVGMTGDGVNDAPALKRSDVGIAMGIKGSEVSKQAADMVLGDDNFHTI
                     AKAVKEGRRILDNLQKTINFFLPTALAQGLILIWALMLNRPLPLSPVQILWVNMVTTI
                     TLSYALGFESPDPEIMKRPPRDPKQGILSGYHLFRIIYVSLLIMVPAYLLAMQFEGQS
                     LQQTILLQNIVLAQAVYMINCRELSKPSLNQGLLQNKALFLSLGILALLQGMVIFLPV
                     GQQLIGTVSLTMTQQLLIGANVILLFLVVEIEKGIMNKLFRKKEKTAREYSR"
     gene            complement(123785..125116)
                     /locus_tag="SCL80_000107"
     CDS             complement(123785..125116)
                     /locus_tag="SCL80_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1106009.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRAMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKENFGQIDELLPFGFAGMVVSFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKKAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQGGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFAGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(125082..126560)
                     /locus_tag="SCL80_000108"
     CDS             complement(125082..126560)
                     /locus_tag="SCL80_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1106010.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITLLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLENPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCL80_000109"
     CDS             complement(126865..128700)
                     /gene="tdc"
                     /locus_tag="SCL80_000109"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEG1106011.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEKVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     GQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKKIAKAMEKKLNEIIQ"
     gene            129170..130597
                     /locus_tag="SCL80_000110"
     CDS             129170..130597
                     /locus_tag="SCL80_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106012.1"
                     /translation="MIEEYIEKNILRQLFLCGQFYVNKEVNLEKLSNLLHVCKTTLLN
                     DINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLSQNSLFLKTCLLYLEEDE
                     PDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPRFTEMERRLLLLNVSYRL
                     GGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILSIGFAISFLRQQVCAVTID
                     SKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLFNLCNYGFHSYQLIAEDF
                     QQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIHLMRSAWDNYQLFMPEKF
                     YLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVIELSGILRLTKEHLTIYI
                     VTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQQSSNRVLCERTLYTPDA
                     VQYENIIPISINTIDRAIISAVQNK"
     gene            complement(130689..131054)
                     /locus_tag="SCL80_000111"
     CDS             complement(130689..131054)
                     /locus_tag="SCL80_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1106013.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPLDVQNK"
     gene            complement(131051..131416)
                     /locus_tag="SCL80_000112"
     CDS             complement(131051..131416)
                     /locus_tag="SCL80_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEG1106014.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(131608..131856)
                     /locus_tag="SCL80_000113"
     CDS             complement(131608..131856)
                     /locus_tag="SCL80_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106015.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            131855..131983
                     /locus_tag="SCL80_000114"
     CDS             131855..131983
                     /locus_tag="SCL80_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEG1106016.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            132040..133734
                     /locus_tag="SCL80_000115"
     CDS             132040..133734
                     /locus_tag="SCL80_000115"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEG1106017.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKITNTLTQ
                     EEEYVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            133738..133857
                     /locus_tag="SCL80_000116"
                     /pseudo
     CDS             133738..133857
                     /locus_tag="SCL80_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            133834..134007
                     /locus_tag="SCL80_000117"
     CDS             133834..134007
                     /locus_tag="SCL80_000117"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106018.1"
                     /translation="MQAFEHRLPRGYEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKGV"
     gene            134385..135518
                     /locus_tag="SCL80_000118"
     CDS             134385..135518
                     /locus_tag="SCL80_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEG1106019.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQSGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYA
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
     gene            135549..135869
                     /locus_tag="SCL80_000119"
     CDS             135549..135869
                     /locus_tag="SCL80_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YrhK family protein"
                     /protein_id="HEG1106020.1"
                     /translation="MPKIKRNTHEIEIGKEEDIEIRGRRFRFYFQNRYTLISLAVDLL
                     TGIFYIFGSLASLTKIPDRYGTYFYLAGAIFLTIRPILRIVRNIFIYDEKKAMKRTSK
                     KKEA"
     gene            complement(135898..136362)
                     /locus_tag="SCL80_000120"
     CDS             complement(135898..136362)
                     /locus_tag="SCL80_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106021.1"
                     /translation="MVTYNWIKLREHPELVKQSANWFSSKWGVPAVVYEESIRTGIAN
                     KESIPQWYVVLDEKETIVGGAGVIENDFHNRPDLTPNLCALFVEPEKRKQGIAKRILA
                     EIRKEMHQLGYEKLYLITDHTTFYERYEWTFLTMVKEESGTTARLYQSDTIG"
     gene            complement(136494..137747)
                     /locus_tag="SCL80_000121"
     CDS             complement(136494..137747)
                     /locus_tag="SCL80_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106022.1"
                     /translation="MDKFVAFMEKHFIPVASKIGAQRHLVAIRDSFMVSMPLMILGAL
                     AVMINNLPIPGFQELMNSIFGGESWKGFGAAAWNGTFAILSVLIAFLLAYHLANGYRK
                     DGVSAGVISLGSFFALGGALGMSSNGLFIAIIVGIISTEIFVRLSGNEKLIIKMPDGV
                     PPAVAKAFASLLPAMITISAFALVAAIFAAFGVDDIVGSFYTVVQEPFMGLANSYPSA
                     LLLAFITPFLWFFGLHGANMVDPLMQTINAPAIEANVNAIAAGHSAPFIVNKPFFDSF
                     VNLGGTGATLGLLLAIYLVGRKNKPYMVVTNLSIAPGVFNINEPTMFGLPIVLNPIMF
                     IPFILTPMVLVSVAYFATSTGLVPAATVMPPWVTPPIIGGVLATKSIAGGVLAAVNLV
                     LSILIYLPFVKVATIQERRKEALDA"
     gene            complement(137776..138093)
                     /locus_tag="SCL80_000122"
     CDS             complement(137776..138093)
                     /locus_tag="SCL80_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1106023.1"
                     /translation="MEDQKNLEAIMGLIMFGGNAKSDAMEAIAAAKKGDFTLADEKIK
                     DAEESLVQAHHSQTEMLTQEAQGNHVEVTLLTVHSQDHLMTSIAFTDLAKEIINLYRR
                     LNA"
     gene            complement(138119..138439)
                     /locus_tag="SCL80_000123"
     CDS             complement(138119..138439)
                     /locus_tag="SCL80_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1106024.1"
                     /translation="MEKKTIMLVCSAGMSTSLLVTKMQKAAEAKGMEADIFAVSASDA
                     DNQLESKNVDVLLLGPQVRFMQNQFAEKLAPKGIPLDVINMADYGMMNGEKVLDQAEK
                     LINK"
     gene            complement(138903..140318)
                     /locus_tag="SCL80_000124"
     CDS             complement(138903..140318)
                     /locus_tag="SCL80_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1106025.1"
                     /translation="MKITIPKNFILGAASSAWQTEGWKGKKEGQDSFIDSWYKNEHFV
                     WHNGYGPAVATNFMEQYQQDVDLMKSIHLTHYRTSINWARFFTDYENCIVDEEYAQHI
                     SEMIDSLIEADVEPMICLEHYEVPAFLLEKYDGWSSRKVVDFYAKYAQIAFDRYGDRV
                     KNWFTFNEPIVPQTRCYLDAVRWPHEQNTKKWMMWNYHKVLATAKAVEIYRSGNYGGR
                     IGCILNPEMVYARSNSPEDQKAAYMYDLFYNRVFFDPMVKGYYTEEFLELCQKQSIYF
                     DPATEDLETIQHHTVDFLGINQYYPKRVRAPRYQWNEETPFHPERYYEVFDLPGKKMN
                     NSRGWEIYPKIMYDIAMYLKENYHNIPWLITENGMGREYEEQYMDEKGIVQDDYRIEF
                     IGQHLHYLIKATEEGANCEGYMLWAFTDCVSPMNAFKNRYGLVRIELNDERSRTLKKS
                     ASWYQTLIDKRELDLPDEIYK"
     gene            complement(140335..141651)
                     /locus_tag="SCL80_000125"
     CDS             complement(140335..141651)
                     /locus_tag="SCL80_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106026.1"
                     /translation="MRGSGMSFMDKLANVLGNVATRINNLRYIMVIKNAFAALIPVII
                     TGAFGTLFSAMVFDSENGLAQISFLRFLEELKPIASAISYVTLSFLTIYAVFLIGIEL
                     AKLNKVDGVFPGIIAVMSYLSVNPTVYELVNENQTVIVENVLAKQYTDTKGLFLGMLV
                     SILSIELYCWLRKQKKLQLRMPDTVPTNVSASFSALFPTILTVSGIAAAGFIIKELTG
                     MYLYDIIYNVVQRPLEGVVQGLPGILLLMFIAQVFWVIGIHGNQMVKPIREPLLLAAI
                     AVNTEAFEAGKEVPNIITMPFWDMYMSMGGSGVTIGLLIAVLLVGKREDMKQITKLSL
                     APGIFNINEPVIFGMPIMLNPILAIPFILTPLITGTIGYFATALGFAAKAVVMVPWPM
                     PPIVNAYLATAGNIGAVITQLVCIIVSILIYLPFVKVANRVENDGI"
     gene            complement(141801..142499)
                     /locus_tag="SCL80_000126"
     CDS             complement(141801..142499)
                     /locus_tag="SCL80_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HEG1106027.1"
                     /translation="MEENKTLVEVTTDKILRYLKEMQLQVGDKLPNEYELSDYLEVGR
                     STVREAVRALASRNILEVRQGAGTFVSDKRGIANDPLGFSLIEDQDRMIRDLFELRYL
                     LEPKMAGLAAEYATKNQIVQMKKLTQKIETSFREGTQEHVQLDIDFHTMIAEASGNVA
                     LLHIIPIINESIALFNKNYDIQHLKIETIDIHTEIVQAIERRNALAAFDAMTVHMAIN
                     RRELQKIIGEKETN"
     gene            complement(142788..143744)
                     /locus_tag="SCL80_000127"
     CDS             complement(142788..143744)
                     /locus_tag="SCL80_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1106028.1"
                     /translation="MEYRKSGHSGLKLPILSLGLWQNFGDYDPIHNQREILRGAFDMG
                     ITHFDLANNYGGPAGAAEKNFGRIFREDFQAYRDELIISSKAGYHMWEGPYGEWGSRK
                     SIISSCDQSLQRMGLDYVDIFYHHRPDPDTPLEETAEALMQLVRQGKALYIGISNYNG
                     EDTKKMTEILKRKEAPFIIHQMRYNMFSRALLEDDLSPVLEEEGLGAITFSPLAQGLL
                     TNRYLHGIPEDSRAHRKEIPFLSEEQVGSTLEKIKALQTIAVSRGQSLAQMALAWNLR
                     QKSVTSVLVGASRLSQLQESVRMMDNLDFSPEEELRIDQILE"
     gene            complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="SCL80_000128"
     CDS             complement(143857..144690)
                     /gene="msrA"
                     /locus_tag="SCL80_000128"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1106029.1"
                     /translation="MDEEQIFIQTLYNLILNPNTRDWERKVLIQTKNDIRENISVKEQ
                     LSKLEATLRPLAIRMNLTPDVMDFYLLITEGFDKEQKYDFSKHAMQDADYQERAVFAG
                     GCFWCMVEPFESKKGILSVLSGYTGGHVEKPNYDQVSGGYTGHVEAVEIIYDTREISY
                     SELLTIYWQITDPTDTFGQFQDRGKQYRPVIFYQVERQKELAEQSKQKLDSSGTFHQP
                     IVTKIEPAGTFWPAENYHQQFYKKQPKRYKKIQQARNQFLIYQRVKNKWQKNIRKNHF
                     D"
     gene            complement(144865..146499)
                     /locus_tag="SCL80_000129"
     CDS             complement(144865..146499)
                     /locus_tag="SCL80_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106030.1"
                     /translation="MKINKKVVILSGLIIVSSIYSFIFFKNTQSIYTTGDLSFHLSRI
                     KGLSTIFTSPINYETFNYTGYGVNYFYPFLTFFPAVMLYWMTKNLIVSYIIYVWLLNL
                     CTTLVAYHYGERFLKQKKAAFLFSCLYIFSAYRTVDIYYRSAIAEAIAITLVIPVLFY
                     AYQIISGKEEKYPSVKLALSMSLLVYSHVLSTLMSTALIIIFIFIRLVSKGFKNADFI
                     AIFKKLFSAAGMTLVLTSAFWYPMFEQMLYQKINKPSVTNLYAHASNVFDSLTEAMNN
                     DLTTYSMGLVGLLSLCIPLILFKKLTNIEKKIYYGTCLTWLATTSLVPWYLLQNTPAK
                     LLQFPWRILSLQIIFSSLILTMIFFKNRRYNKTRELFYLFMTVLLLITLTVSSKKNFN
                     QKIISQPGHIVVNKETVEAYTTKGMDYFLYDYSPIASLENKEHIIKHEAKIGSQWVNL
                     PYKTTGSSIIYRVTNEKSQVITLPVYAYLGTNVEINHQPVKEQVNANGLVQISGVKGV
                     ADIKVSTSYTVTTIISYLVTLTAYLCLFFKIVFHKR"
     gene            complement(146486..148174)
                     /locus_tag="SCL80_000130"
     CDS             complement(146486..148174)
                     /locus_tag="SCL80_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106031.1"
                     /translation="MKISKKAFLLVVLILLSTLYSVNFMRNAQEIYTTGDLSFHLSRI
                     KGLSSIFEGPINYTTFNNYGDGLNYFYPFLTIIPAVVFYGISNNLILSYVLYIWLLNI
                     CTILISFYYGEKFFKKIDAAFLFSCLYTFATYRTVDIYYRSAIAEAIALTIIPAVLYY
                     AYGLIFNREKKVIALSVSISLLVYTHVLTTLLCSALIACMIIVRLFLSKQIKNDIKFV
                     VVNLVKAVFLTCALTSYFWYPMLQQMNYQEIHSPFKTDLQLEALSISDSIIQALNNDI
                     TTYTMGLLGLVALIFPLFILKEMEKKEKIIYVGVVSTWVCVTNLFPWFLFQKTPISLL
                     QFPWRILGIQIFFSSMIITLVFMKKTMNKKYSWLIIGATVVFLLVTSIAAKENYAKVI
                     QSKHGHIVINEETLPRYTTSDMGGLYDYAPTEAIKERPHLEKHEVKVGESWEKGKYKA
                     ADNNITYTVASNKKQKVTVPVYAYLGTQVKVNGKVVNDSYKKHGLVNVDVNAGENKIE
                     ITTKYTPTALMALWISILTYCLVVYSSFKRFAPNKLKDSRYVTKKEINKKNENK"
BASE COUNT        43873 a        30729 c        25743 g        47913 t
ORIGIN      
        1 ttttttgcct atcagtgcaa aagcatttcc tgtgcaattt cacgtccatc cattgtatct
       61 ggataaaatg ttggccaatt ctccatttct ttcagcaata cttcttgact ttcatttcct
      121 gcatataaat gaaaatgttc ggagtcagcc ggtgcaatcg tgtgatcact gaattgaata
      181 tatctaaaag cgccactttc cttatcttct gcttcgaata gataccttac gccacggttt
      241 cctgcctcgt aggtcaagat ttctttccct acataacgat aatttgaacg tttcgttgtt
      301 ccatccttaa acgtaaatgc catctctttc tcactgatct tgattgaagc aatatctgtt
      361 tgatagccct tcgtataata ttctttatat tcttgtgctg tcttatcctg attcaatttt
      421 tctttatatt caaaaacagg atcaaaagtc ccatcttcta catacgtatc tactgtttgc
      481 caagttcctg cccaatctga aagagagcga tcttgtacat cctcgtcttc aaaataaccg
      541 ttgtagacgc tcttttcttt tttgggggtc aaagtatctt ctagcgggtt ttctgtctct
      601 gtcgtttttt tcaacgcttc gaggtttgct tccatgatag aaagatagtt ttctccattt
      661 tctatttgtt cattcgtcaa tccttccaaa ggattcaata cttctaaaga tacacctgct
      721 tcgtttgcta acgttctggc aatactatct ttggcgtttt cttcaaaata aatatagttg
      781 attccatgtt ctttaacaaa gtgtttcagt tcagcaattc tcgccgcaga cggttcttca
      841 ctagaagaaa gaccagcaat cgatacttga ttcaatccat attctaacgc tagatagttg
      901 aatgcagcat gttgcgtgac aaaagatctt tgttttgctt gcttaaaggt ttcttgataa
      961 agctgatgga gtgcttgaag tttctcggta taggctgctg cattttttgt ccatacttcc
     1021 tgtttttctg ggtaagcttc aatcagctga tcacgtatgt ttgctacctg cttgattgct
     1081 aaacttggag ctagccatac atgtgggtct agctcatgtg tatggccttc ttccccgtgt
     1141 tcatggtcat gatcatgtcc ttcttcactt cccggaagta aaagcatacc ttctgtcgct
     1201 tttatcgtat ggacattgcc ttcttgtaat gttttttgga ttgcggggac ccatgtttcc
     1261 atgttttcat cattataaac aaagatatct gtatcttgga tctttgccat gtctttagca
     1321 gaaggttcat aatcgtggga atcagtccct gctggaatca ataactctac ttctccttca
     1381 tccccgacga ttgcttttgt aaaatcatac atgggataaa aggtcgtcat cacgctcaat
     1441 ttttctttac tgtcctcggc tttcccactt gccccgcaag cagttaacat acctgctaaa
     1501 accagcatgg ccaatatacc tatcaatcgt ttcatttatg acactcctct tctcatcttc
     1561 tttcatttga atgttccgat tcgcttttcg caaaccgtaa tagttacgtt ttagcaacag
     1621 gatttaattt atcatatggt taaaagaact gtcaagcgtt ttcttatttt aattgatgag
     1681 aaagtttata tgaatgattg acgatatttt tgcaatcaac ctctatcatt ttccttttat
     1741 acccgctttt gtctataatg aagttataga taaaggagga aaaagagtga aaaaagaaac
     1801 agatacaacg aatcgtttga tccgcttttt aggaggaaaa acctcttact atgttttagg
     1861 aatagttatc ttacttgcgc tgatcattta ttttttccat cagatctcgt ttatattcaa
     1921 gcctgtcgga atcattatcg ctactatctt gcccccatta ttgtttgcac tgatcttgta
     1981 ttatcttttg gaaccagtca tcaaacgttt gagcaagcac ctttcgagga acttgtcagt
     2041 cattatcgtt tatgtgatta ttttattttt attggggctt ggaagcatat ggcttattcc
     2101 ttttcttgaa gaacaggcaa agacattgat cagtagtttg ccagatttgt ttcaagattt
     2161 tcaagagagt ctccgacaat ttttggagaa aacacctttt gccgattcat ttaatcaatt
     2221 ttttgcttct attgacgacg tgacgtctga tatagctgga tttattggaa attattggga
     2281 aagtggcgca caacggatca gcaacatttt ttcaactgtt acagctattt ttattacttt
     2341 atttactggt ccaattgtcg cttttttcct tttgagagat cctcaaaaat tttatcagtc
     2401 agttttagcg attgtcccac cagcatttcg tacagatttt aaaaatttga caaagattgc
     2461 taatcaacaa ttgggttcat ttctaaaggg gcaaatcatt gcttcattta tccttggagc
     2521 agtctactgg gtttgtttct tactgatcgg attagaattt gctagtgttt tagctatttc
     2581 tgctgggttg ttatgtatca ttccatatat tgggccgttt atcgccttct ttccaggttt
     2641 gatcattgca ttccaagact ctacttttat ggctgtcaaa tttgtcattg tctggtttgc
     2701 cgtacaactt ctccacggtg acttggtgat tcctcgagta atgggtgatc gtttacagat
     2761 ccatccgatt acgatcttaa tcgttttact tgtcatgggt gatctaatgg gaattgtggg
     2821 cgttattttt ggtattccta tctatacgtt agtcaaatta ctcgttactt ttattttccg
     2881 taaattcaaa caacgctaca ataaatttta tggcgataaa ggagagtatg aaaactcttc
     2941 attttctgaa gaagattatt ttaaataaaa cacaaaacgt cgatcttacg agaatagctc
     3001 gtaggaacga cgttttgtgt ttttgtttta atttttcagc aaagcaattg cctggtatgg
     3061 ttttagctcc agactttgat gaagaacagt atcggaataa ttatcgataa gaatattggc
     3121 ttctgtgaat ccttctggga tggcgatcct tgtatcttta tcatagaaat ttgttaatac
     3181 aagcagctgc tgtccttcaa attggcgaag aaagccataa acttccggat gatttgcttc
     3241 ataagcatga taatctcctt gtgcaacgat tggatatgtt tttctcagcc gaatcaattt
     3301 cttgtaatag gacaaaatag aatcttcaga agatagttct ttttctacat taatctcttc
     3361 tagcttgcct ccaactttta accaaggttt ccctgtagta aatcctgctt gttctgtgtc
     3421 tgaccattgc atgggtgttc gagagttatc gcgagatttt gctttgatcc gacgaaacgc
     3481 ttctttttct gtgaatcctt gttcaaggag taacttataa gcattcaaac tttctacgtc
     3541 aatatagtcg gaaatcgatg aatagtctgg gtcgatcatg ccgatttctt ctcccatata
     3601 aatatagggc gtcccacggt tcaaatggat catggttgct agcatctctg ctccttgttt
     3661 tcgatactga tcgataacta caaatcgatt gagcgcacgt ggctgatcat gattattaaa
     3721 aaatagagca ttccagccgt taccctcact catattttct ccccatgtat gaaatagtcg
     3781 ctttaattct gcaaaatcaa atggaggagt agaccattta ttcccatttt cataatcgac
     3841 tttcaaatga tggaaattaa agaccattga taattcttct ctactaggtt ctgtatacaa
     3901 gatacaattt tcgatcgtcg tagagctcat ttcaccgact gtgatgatag tggaatccgt
     3961 cccaaacgtt gcttggttca attcttttag atacgtatgg gtgattggtt tgtccgtata
     4021 ttcaggtttg ccatcatttt ctttattatt tttcaattgt tcgtctttac cgataacatt
     4081 gataacatcg aaacggaatc cttttacccc tttttccatc caaaaacgaa caactttgaa
     4141 tagttcctct cttactgctt ggttccgcca gttcaagtca gcttgtgtcc gatcatataa
     4201 gtgcaaataa tatttccccg tatctccaaa tggcgaccaa gcacttccac caaattttga
     4261 tacccaatcc gttggctctt ctcttaaaat gaagaagtcc tgataatgct ggtcccctgc
     4321 tagtgctttt ttgaaccatt catgttctgt cgaaacgtga ttcagcacca tatcgagcat
     4381 gatttctatc cggtatttct ttgcttcggc tattagctgt tcgaattcct ccatcgtacc
     4441 aaagatagga tcgatggctg tgtaatcaga gatatcataa ccgttgtctt tttgagggct
     4501 tggataaata gggtttagcc aaatcatgtc gatgcctaga tcattcagat acgggagttt
     4561 ttctttgatt ccttttaaat ctccaattcc atcaccatta ctgtctttgt aagatttggg
     4621 atagatttgg taaatgactt tgtctttaaa tgtcatggat atttacctcc attcaactga
     4681 tttattacct accacagctt atatatacaa gttatcattt attattataa ctgaacagaa
     4741 agcgtttgta aaatttatct ttatacattc taagaggtct gttgcccaat aaaaaacaag
     4801 agatcagaca tgtatttgtc tatcctcttg tttaaaatag caaaatattc ttgtttcttc
     4861 tataaatggt gttcaaaagc ttttatctac ggcaatcagt cttctcaaat gtaaaacatg
     4921 aggagctaat tttatttgag tattcttttt gctcgatgca ggacggcttt tttacaacct
     4981 ctgattcatg gtgttcaatt agcagctgac cctcgttatt agctcaaaac gctgatttca
     5041 caacctctgt ttttattcat tcgatttcaa cgcatcaatc atatcgatat gctttaattt
     5101 gaaatgggta acaatcatca caataaccgt aaatacgata gttaatcctg ctgataagac
     5161 ataagcccac catgtgatca ctaatggaaa gatgacattc tccatagacg cttgttgcaa
     5221 gatgaagtca gtcaatatat atcctacacc aaatcctccg atgattccta aaagagtaaa
     5281 gatgatattt tctcgaacaa tgtacatcgt gacttcttta tcaaaaaatc caaggacttt
     5341 tattgtagat aattcgcgta cacgctcgga aatattgata ttagttagat tatacaagac
     5401 gataaaagcc agcagtcctg ataatacgac aaagattaag acaaccgagt ctaagttcgc
     5461 cattgattct tcttgcgttt cgatttgttt ggacatgaaa gacgtatttg tcacagcgtc
     5521 tgtggcaagc agtttctcgg aaagcatttc ttcttctttt gttgtcatct tatctgtacg
     5581 cagcaaaaat gcattatcca tcatcgattg atcagtaact gaagcataat attcagggga
     5641 catataaata aagctaccta gatagttttt cacgatttct tttacttcga ctttccattc
     5701 tttttgatct tcatcataaa gcgaaatatg atctcctgta gataagtcaa atagttctgc
     5761 tactttttgc gtaatagcga ttcctgtttt aggcaaggag cgtgggttct cttcaagatc
     5821 agttaaatgg ataaaacgat ccaaatcagc tgttttactt ggtacgatca ctgtcagact
     5881 ttgcgaagca tgtccttttt ttctcaattc tacggtttga gtatgaatag ctaggatctc
     5941 atttactttt tcttgcttct gcagcatatc cgctgctttt tgacgactat cttccgtgga
     6001 tgaatccaat gtaacgattg cttgatagtc aatgatcggt ccaaattgtt tggctgaaac
     6061 agaacttaga gaatctttca atcccacgcc tgcaaccatc agcccggcac atccagcaat
     6121 tccgataatc gccataagca ttcttgactt atatcggaag agatttcggt aactgatttt
     6181 ttgattaaag ctaaggcgtg accacagcgg agtgatgtat tcaagaaaaa tgcgtttccc
     6241 tggcttcggt gctttcggtt gaagcaatgc agcaggccgt tctcttaaat ctttgaataa
     6301 aacgatcatt gcagcgccaa atgcggcaat gaagaatgcg gcagctgctt gtaaaatcgg
     6361 cggccagtca taaaaaataa gcgcactccc aatatcatac cgctcgttag acagctcaaa
     6421 aatgatcctt ggcaaaagat tcgttcctaa tatcgttcct aaaacaatac ctatccctga
     6481 ggctagactt gcatagattg catatttttt tgcgatttca actttcgtat aacctaacgc
     6541 ctttaatgtc ccgatttccc tgcggttttc ttctaccata cgtgtcattg tcgtaaaggt
     6601 gatcaatgcg gcaataaaga aaaagaaaac aggaaaaaca ttggcaatcg cagcgatacg
     6661 atcagacaga tcaccaaatt cttgaaaacc gacattttct gaacgttcat tgtataggta
     6721 tgatggttta gttaaatttt caattttttc tgcatttcga ttcaattctt cctctgcaga
     6781 ggccagttca cgttctttct caactaattg cctttcttgt tcagcaagtt gagcttcttg
     6841 ttgaggtaat gtttgaacag cgattgttcg ttgcgcttca ggaagctgct ggatagttgc
     6901 tgactgttgc gctaactgtt ttttagcatc ttctaaccgt gttcttgctt gcgttgtttg
     6961 tgctttgcca tccgctattt tttgctgtgc cggttctaat tcttcgttgg ccgatttttt
     7021 cagctcttct aagcgttctt cgggtcgatc ttgtaaatac ttttctgcct tttcttgatt
     7081 ttgctccatt ttatctttgt aagctttgct gtacgtattc aaattcttta cgtttgtaaa
     7141 atcaagatat aagacagaat aaatatctgt tttaaaaacg gattcaggta aatagacaaa
     7201 ataatcgacg ctaccatttc cgacgttcgc gtatcctctc tccaaattgt tgatgaacaa
     7261 tggagaacgg acaaagcccg tgatggtata ctctttttct ttcaattcat ctgaatcaat
     7321 ctgataaata tcaccgattt tatacccttc atcttccgct ttttcatcca atacaagctg
     7381 gtttggtttt tccgggagtt ttccatcagt taattgtagc ttgttctgct tgttattttt
     7441 gttataagaa aatatttgaa tcacttcgtt tttgccagta ttggaataat agatctgttt
     7501 tcctgtttct acttttgcat ctgggatctt ttctgcttga gcaatatctt tttgtgtcaa
     7561 gccaccagta gaaacgattt gcaaatcaga aagtcctgct gtaccaacat agtgatccat
     7621 cgttttttgc atgacaggtc cagcagtttt gacacctaca aataaaagtg ttcctagcat
     7681 gacaataagg atgattgcaa tgaatcgtcc tttagattgc cgaatttcgc gaaaacctgc
     7741 tttaagatat gttcttgttt tcataaaatc cccctaccat acaatctcat caatggatga
     7801 aggatgatca tttaattcaa cagaccttac tttagcatca ttaatgtgaa tgactctatc
     7861 agcgatgggc gctaaagcag aattatgtgt aatgatcaaa acggtattgc cgtgttgtct
     7921 gctagcgttt tgcaataatt tcaatacttg cttgcccgtt tcaaaatcca gcgcgcctgt
     7981 cggttcatca cataaaagaa gtttgggatt tttagctagt gcacgagcaa ttgaaaccct
     8041 ctgctgttcg cctcctgata attgggacgg aaagttattc aatcgatgag ctaggcccac
     8101 ttgcctcaat acctctacag gatcaagcgc atcaggagaa acttccgttg ccaattccac
     8161 attttctttt gctgttagat taggaacgag attgtagaat tggaaaacga accctacatc
     8221 tgttcgtcgg taagcagtaa gctgtttatc tgaaaattgc gcaatatctg tgtcatcaat
     8281 aatgatctga ccttcatcag gtgtatccat cccacctaaa atattaagaa tcgttgactt
     8341 tcctgctcca cttggaccaa gtataatcac taattcgcct ttttcaattg aaaaagaaat
     8401 atcgtcattt gctgtaatgg tcgtttctcc cattttgtat cgtttgtatt cattttttac
     8461 ttcgacatac ttcactctga ttcctccatt ttcacgatat ttctcgctat atctatgcat
     8521 cgaatgcgtt ttctcacact tatttatttt accactgaat ttctctagac taaagaaagg
     8581 aatcctttaa tttagttgac aaataaacga tttatgattt acttacataa aaaacattgt
     8641 tatttgaaag aaggaacatc atgaatgatt taaaggaaaa tatgattttt gatttatcaa
     8701 aaactgcttt tgtcgcgatc gacatgcaaa aagagatcgt aaataacggt acactctctc
     8761 cacacactgc agcatctatt ctgacaaaca atgatttgtt agtaagaaca ttgaaacgta
     8821 ccgctgcctt gaaagtttta gtgaacgtag acatctccac tttcccttat ttgagtcaac
     8881 aaactgatat gggtggaatg actgcttctg tacctccaga attcactgat ttacttttga
     8941 aggattcttt gaaagataca gataatatgt tgactatcaa aaaatacaat ccaagtgctt
     9001 tttttggtac ttctcttgat cttcaacttc gccggcgtgg aatcgaaact atcattttaa
     9061 gcggtgtagc gacaacaaac ggtgtatatg ctactgcatt agatgccttt caacacgggt
     9121 accatatcgt gcttgctgaa gatgcttgca gtgatcgtga caaagaatcc catcaattgt
     9181 tcataaaaaa gatatttccg aaaactgcac gagtacgctc aacgaaacaa ataatcgaag
     9241 ctatccaaca aagcaaataa gtactggcac acgtgatttt catttacttc tttttgattt
     9301 catgggaaaa taaccacagg atttagatag gagtatgaac atggtgaaaa aaaagatcac
     9361 aatcaaagaa gtcgctaaac tttccggcgt ttcaatcaca actgtctcct tgatattaaa
     9421 tggaaatacg gagaaattca gtccgaaaac tgttcagaaa gttttagctg ccaaagaaga
     9481 attaggctat cagcctgact attttgctag acaaatgatt accaaggaaa cgaaaacaat
     9541 tggtgtactc gttcctgata tcacgaatcc attcttcagt acactgatgc gaggaattga
     9601 ggatatcttg tataagcaga atttcgtcac gatattatgt aatgctgatt ctgatcatca
     9661 aaaagaaatc gaatatctag ccgagctcac acgtcgaggt gtggatggat tcattatcgc
     9721 gacttctgct gtctctacag atgcaatcaa cgaaaatttg aaaaaacaag gccggccctt
     9781 cattgttttg gatcagaaaa aatctgaagg atttagtgat gccgtacgaa cagacgattt
     9841 tcgcggtggt tacctagcgg gcatgcactt gcttagtcta ggacatcaaa cgatcgccct
     9901 tgtctatcct gaaaatccac cggaaaatgt ccacgctcgg attgaggggt ttaaatctgc
     9961 tcttgatgtt taccaaatcc ctcacgacca actgatcctc ttacctacac agttctcaaa
    10021 acaaggtggc taccagatca cagcagaact tcttgattct gctgcaactg gtgtgtttgc
    10081 tttaaatgat gaattagctt ttggattata tcgcggatta gaggaagctg gaaaaagcat
    10141 ccctgaagac tacagcatta ttggctatga taatatagat atgtgcgagt atatcaagcc
    10201 gaaactgaca acgatcgcac aaccaatctt cgaattggga caaacatctg ccaagttatt
    10261 gttagatcgg atccaattcc cagaaaaaga atgggaagaa aaaagattac ctgtacgatt
    10321 agaaaaaaga ttttcaactg cacccttgaa atagctttca aggtgtgcta tatttttatc
    10381 gaactattaa aacgttttac taaaacgttt taaaaaataa ttttcccatc aaaataaatg
    10441 agatagttgg aggtttgtaa aatgaacacg gttacagtaa ttggaagtat taatatggac
    10501 actacccttc gtttaaccaa catgcccaag ccaggcgaaa cgatgcatgc gcatgagatt
    10561 tttcacgctg gcggaggtaa aggtgccaac caagcagtgg cagccaaacg ttcaggcgca
    10621 cggacatctt ttattggagg cgttggggct gacagcgaag gacaacagtt attagatcta
    10681 ctgacaaaag aaaacatcga tacatctggg atagctgaaa tccaaggcgc cacaaccgga
    10741 caagcaatga ttatggtaga cgcagcagga gaaaatagta tcttgatcca tgcaggggca
    10801 aataatgctt tccatgaaca agaagtccta aaaaacaagc aactgattac aaacagtgac
    10861 tttattattg ctcagtttga aagtagctta gatgcgacga tcctagcttt ttctattgca
    10921 aaagacgcag gaaaaacaac gatcctgaat cctgcaccag ctcgtgaaac gattcctact
    10981 gaactacttg aaaagacaga tatcattata ccaaatgaaa cagaaacaga aatcattaca
    11041 ggaatcagag tgacagatca taacagtttg gtcgcagcag ccgaaaaact ccatgaatta
    11101 ggtattggaa cagtaatcat tacactaggc agtgccggcg ctttctatca tacagaaaaa
    11161 gaacacggta tcgtccctgc ttttaaagtg gatgccgttg acacaacagc cgcaggtgat
    11221 acatttattg gcgcattaag cagcacactt cagccagacc ttagtaactt gaaagaagcg
    11281 atcctttatg gaaatctagc ctcttctgtt gctgtgcagt cttatggtgc gcaaccttct
    11341 atcccttacc gcgaagcttt agaacaatca gcagtctaaa attaacaatg agaggaagaa
    11401 acaaacatga aaaaaggaaa agtaatcaat tcagatattt cacgagtaat cgcacaaatg
    11461 gggcactttg ataaattaag tatcggggat gcaggcatgc ctgtcccctt tggtactgaa
    11521 aaaatagatt tggctgtctc aaacggtctt ccaagttttt tatctgtttt agaaaacgtt
    11581 ttagaagaac ttgaggtaca atcgattgaa ttggcggaag aaataaagac aatgaacccg
    11641 gaaatattgg aaaaaatcaa attattgtta ccagatacac cgattagctt tatcccacat
    11701 gaagaaatga aaaaagagtt gaataactgt catgcattta ttcgtacagg cgaaatgaca
    11761 ccttacgcca atattatatt agtgagtgga gtggtcttct aatggaaacg acaatgaatc
    11821 ctgaacagat ggtactgtcc gagcagattc atatgctgat tgaacagttg ggaggaaaag
    11881 acaatattga ttctgtcctt ccatgtgcga ctcggcttcg tatcaaattg aaaaaacagc
    11941 cagtagccaa tgcagaaaaa ttaaaacagc aaatcccttc cattaagtat ttcattttac
    12001 tcgatagata tgcagaaata atcatggatc agggattggc tcctgcttac aaggaattga
    12061 cgcgtcaaat cgatcattca gctccgaaag aaaagctaaa tccgatcgaa aaactatttg
    12121 gtttgatatc ggaaatcttt acgccgatca ttccgctgtt tgccggttct ggtatattaa
    12181 aaggtctggt tgttttagcg actacgattg gctggcttag tgaaacaagc ggaacttatc
    12241 atattttgtc tgctgcgtcg aatgccgtct tttattttct gccaatcttt cttgcttttt
    12301 ctgctgccaa aacgtttaaa gtgaatggat atattgcggc tgtcattgct gcagcactga
    12361 ttgaaccgaa tctgacctct ttactttcag acagtggcaa agcagctact gattttctgg
    12421 ggattcctgt agtactgatg cagtatacgt ctactgtcat gccggcgatt ttaggtattt
    12481 tcttttattc ttttgttgaa aaattcctca aaaaatatat tcgcgaaaat atgcaattag
    12541 tatttgtacc tttactttca ttagttatca cggttccttt gacgttaatg gtattcgggc
    12601 ctgcgggtgt ttaccttggc gaaggtttag ctgccgcaat tacttggatg atggatatta
    12661 acggaccgat cagtggcgca atcatcgctg gtggttggaa tattttggta atatttggta
    12721 tccaatgggc tgtaaaccct gtcatgatca gtaatatctc tgcttatggg ttcgaccgaa
    12781 tcgttccgtt gacaggagca gccaattttg gaatggctgg tgcagcttta ggtgtatttc
    12841 tgcgaagtaa acgttcaaaa acccgttcga tctccggttc tgcttttgct tcgattttac
    12901 ttgcgggagt gaccgaacca actgtttacg gaattgcgat cccattgaaa aaaccatttg
    12961 ttgctgcttg cattggtgca gctgctggtg gagctgtaat gggatttgcc caagtgaaag
    13021 cgatcgcttt tgtatttggt agtctgacca cattgcctgc attcatttcc ggtacttttt
    13081 tctggtactt agcaggactg gctgttagtc tagtcgtagc aatgatcact actctcgtta
    13141 gcgggtttga tgaagaccta atgtcttatg aatagttaat gaaaaagact gaagtatgag
    13201 atgtttatca catatttcag tctttttctt tttaggatca ttatttttat aaatttttgt
    13261 gatactttcc aaactttggt ataatttaca tatgcataac catttttatc cccacttgtt
    13321 tatccccaaa caaaggtggt ctatgcaata aaatgaacgt caaagaaaaa cgaatgaatg
    13381 ctcgttgctt atttacaaat tgttttattt agaatacaca gacagattta tgaaaagaaa
    13441 aagcttttcg acacgattat ctgttccaaa aaatgtctaa tgaatgctga ataagacaga
    13501 aaatcaatga aaggaaaagg actagtttat tatgaaaaat aaaaaaacga tacgtttgct
    13561 agctgctact tggctgattg ctccgtctgt tttgtcgacc aataccgtat ttgccgtaga
    13621 taatcaaata gctgatcaaa cagtcacaac agatacgact acatccagct cagctactag
    13681 tgaaacaacg gattctttta cagaagaccc aactggaaca actaactcaa caacaggatc
    13741 gaccgagcca actgaaattg atccggctga tccatcagaa aatgaaaagc cagccgagac
    13801 atattccctt acaattgctc cttctttgat tggtaagatc caactgactg cttcgacagc
    13861 ggattcccct gaacagcaac ctttggatat tacggaaagt ggaacggtag aaggtttatt
    13921 agcggaaacg aaggtaactt acactattac gccagctgaa ggtgaacaaa ttgcttcagt
    13981 cgctgttaat ggtgccaaag aagtaaatta tgatgcaaag acctttacag taggtacgaa
    14041 caatttatct attactgcag aatttaaagc tggaccggat acttcaacag agaaaccaac
    14101 tgatcctagt gaagaagtcc ctagtacacc agatccggaa acgccaaaca aaccagatgt
    14161 gggtaataac aacaataata atggttcaaa taattcaaac aataataaca ataattcgaa
    14221 cagcgggaat tcttcgaatc aaacaaaccc atctggatcg aataacaatg gaaatactca
    14281 gagagaacca aaccatccaa acaatggttc atcgaatggc ggaacgaaca atgcttcatc
    14341 tcgtccaaat acagcaaatc aaagcggtat agagaatcca tcttctgctt cttctgattt
    14401 tgttgtaaaa tcaccaattg atgctgtatt gcctacaaat acaacgaatg tccaacaagc
    14461 aatcgttcga gaagcattca agcatcttgg aaaaccatat gtatggggag caaaaggtcc
    14521 aaatactttt gactgttctg gtttgactta ttacgtatat atgaaagcaa ccggtcatta
    14581 tatcggtggc tggacaggcg aacagcaata tgcaggtacg cagattccag taagtcaggc
    14641 ccaacctggc gatctcgtct tctggggacc ttcttccggt gttactcacc atgtaggtat
    14701 ctatattggg aacggacaat ttatccatgc gcctcaacca ggagataaag ttcgagtaac
    14761 ttctatttca gatttcacgc ctgattttgc tgtccgagta aacttagcag gtttacctgc
    14821 tgcttcaggt tcgctgacag gcggctcatc tattttagat ggattagatg gctcgttcca
    14881 tttcagtcaa aaccaaacaa ctgatcagtt cttgaaaaag attgcagatg atgcacagga
    14941 aatcgggcaa aaagaaggaa tctacgcatc agtcatgatg gctcaagcta tccttgaaag
    15001 tggatctgga aacagcttat tatcaagcga accaaatcat aacctttttg gtatcaaagg
    15061 atcatataaa ggaagcagcg tgaccttcaa tacacttgaa caagatagtt ccggacaaag
    15121 ctaccaaatc cgcgcacaat tccgtaaata tccttcttac aaggaaagtt tggaagacta
    15181 tgccgacttg atcaaaaatg gactgactgg caatcctgat ttctacaaac caacttggaa
    15241 gtcggaaaca aaagactaca aagaagcaac caaatatttg gaaggacgtt atgctacaga
    15301 tcgccaatat tcacaaaaat tgaacgcgat catcgaagcg tacgacctga caaaatatga
    15361 tgaaccgaaa aaagaagcag caacaactga agaagcaaac gctgaaatcc tcaatatgtc
    15421 taagcgattc gatgtgccaa tcaaatggac aacagatggc gtgccaatca caggattaga
    15481 attttcaacg ataaaacgcg tcaacaacgc ttcttccatc ttccgtgtcg cttcaatctg
    15541 ggatttgtgg aacaatttta cggcaagaca gataccagaa acaactactc gtacagttaa
    15601 agctgcaact gttcctgtac taaccttgtt aagtttagac aaatcattct ggcttgatct
    15661 gcctttaaag aaatagaata aattaaaaag gggatgtgac actgtcacac tcccttttta
    15721 atttattcta tttctttata tttattttgt attccgttac aaactcgcgt acttggttag
    15781 gttccagagt gacatctcca aaactttcaa gtcgctccgc tcctggcagt tcttgagtct
    15841 caaatgtgat accgccatga tttactaatt tattcccgcg catttccggt ccatcttctc
    15901 caaaatttgc tgtaaaaatc acgattcctg aagcatctgt ttttacttgg atcgatactt
    15961 tttcatctgg gcttgtcaat tccgcagcat ttagtaaacc agattgtttt ttcaacaaaa
    16021 atggatgatc gatgccgccc actatcattg attggcgaaa atcactggca aagacctcac
    16081 ctaatttttt aggacgttgg aagtcaaatg ctgtgccact tactgaaact acttctcctg
    16141 taggcgttgt atctttattg agcgtagcaa ataatcggct attcaaccaa agtatatgtt
    16201 cgtcgattga ctgtgctgga tcaccggtta aattgaaata gacatgattt gtaggattga
    16261 atactgtttt gcggtcactg atcccttgga ccgtcactcg ccatgtatca tcgttcgtca
    16321 gcgtataccg tacttctaca tctaaagttc caggaaatcc gtttcccata tctgggctag
    16381 tctgatagaa aatgaccgat gcttcgtttt caccattgat gactgtataa tgccactgct
    16441 tggcttcaaa gctaggtgca ccaccatgta aacaatgacc agtgtccggg tctgttttta
    16501 cttcgaacgt ttcatcatct aatttgaatg tgccattcgt aatccgtcca gccatacgtc
    16561 cgattgaagc accgatataa gggtcttttt ctagatattc ttttgcttca tcaaaaccta
    16621 agatcaactc tcgtttttca ttatcgatta ttgtttgaaa atgaacaacc cgtgccccaa
    16681 gatcactgac agacaaagat gtcccatttt gattcgtgat cgtaatcaag tgatagtttt
    16741 cgccaaaatt ttgttcattg atttccatgt tccttgcacc ctcttttctt tcatttgcac
    16801 tgattccagt atacatggaa accgttttct taacagctta ttccgtgagt aacgagaaaa
    16861 aatttactat attctaataa aagaccgtga aaaaaaggtt gtgacaaaaa tattgtcaca
    16921 acctcttttt ccgaacaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca
    16981 aacgaaaaat atgaggagct atttttgctt ggctgttctt attacttgat gcaggacagc
    17041 tttttcacaa tctctttttc aatacgattc cagtatataa ccatgtcgtc agtacatagt
    17101 agatgaaata caatacgaag aagatcgtaa acggaaccca aatatctttg tatggatttt
    17161 gcatgaatag accaaacatt tgtaatccga ataagacatg gactacacct aagattcctg
    17221 gtgctaagaa taacacgcct acttctctac ggatcgattg tttaagcaac ccgcgtcgtg
    17281 ttcctatctt ttcaagcatt tcataacgag caatgtcact cttgcttcct gacaagatct
    17341 tgaacatcaa acagctagca agcatggtca agaaagctaa acctaggaag aatcccataa
    17401 attcaaaacc agagaataga ccattataca cttcatacat ttcatatttc tggttgctgt
    17461 actctacttt ttgcaaagat ggattgttca ttttattttg agcaacaagt gtttcgattg
    17521 gtttcaggtt tgcttggaag tcttttacct gaactagctg taaagtggat tgtggcaagt
    17581 tcagttgatc aaattctgat tgactgacta ccttgatttc tttttcttgc tgttccggca
    17641 attcatagct tcgaagttcc tcttgtacag tcagttcatt cattttttta gcaggaactt
    17701 tttctttcgt gatcttcggc ggtgttccat cttcatgttt tatcatgatc aatggttgag
    17761 ccgcaaattc acttgcatta tagtaaatcg ttcccgcatc ttctttttga gtataagaag
    17821 cagaaagtgt tggtgaaagt tctttgattt tttgctgatc ttctgcccga ggattattca
    17881 tcactagatc atatccagaa ttgttttctg tcaagtctgc aatttcattt ttaaaaccta
    17941 atcctaccgt caaagcgccc agtgccaagg caaacagcat agcgaccatt gagagcattt
    18001 gtgtatattc acgaatacga aagtttaact gtgacaatgt gaaattgttc aattttttca
    18061 atgaaatctg ttctgatttc tttagtaatg ataaaaagaa aatgatcaca gaatggaaaa
    18121 tgaaataagt tcctaatatg atcgtaacta aggctacccc tataccaaag acttgatagg
    18181 tccccacgtg tttcatcata tagtaaccgc agcctagaaa agcaattccc agtactactt
    18241 caaggaaaaa caagaattta tgctgcttga ttcttgttgg tgtctgatcc gcacgaatca
    18301 atgctaaaat cggttttttg acaatcgaaa aagcattgac aacagcagct aataaaaaca
    18361 aaacagcaaa aaacaaaaca gtgatcaaca acccgtgaat actaaaaggt gtaaaatgcg
    18421 aaatattgag atctaactga tccactaata atcggtggac gatacttgtc aatcctacac
    18481 cgacagctga acccactaaa gtagctgcga tccctactaa gaatgtttca gaaaaaatca
    18541 tctgtgcgat ttttctgcct tttgctccca gcatcataaa cattgcatag tctttttgcc
    18601 gcatcgtcat caaaaaggaa tttgcatata aaatataaac gaatgtgatg atccctagta
    18661 aaacagaacc gaatcggaaa atgataactg tagccgcaac gatcgtatta cttttcaaaa
    18721 aagcttcgtt gcttgccatt gattcaaaca tgtaaaaaat agcagatgcc ataactaatc
    18781 ctgagaataa caccaagtag tctcttaaac ggctcttgat cccagtaagt gataatttcc
    18841 aaagcatagt caatctcccc ctactgttcc aagtttccaa gaatagatag tatttctcga
    18901 tagaaggaat cacggctttg atctccacgt ttcacttctt gatggatacc gccatccttg
    18961 atgaataaaa tacgttggca ataacttgca gaaaacggat catgcgtcac aagaagaatc
    19021 gatacatgat cttttgtatt caattcgtcc atcgtatcta aaagatcccg ggcacttttg
    19081 gaatccagcg cacctgtagg ctcatcccct aatagaatcg ttggttgtgt gatcaatgca
    19141 cgtgcggcgg cgacccgttg tttttgtcca ccggaaatct cagaaggata gctttccaaa
    19201 atatgagaga tggataaacg ttctgcaatt ttttggacac gttgcttgat ttcttttggt
    19261 ctgacgcctt gtaaagaaag aggaacagcg atattttctt gtgctgtcaa gctttctaat
    19321 aaattgaaat cttggaaaat aaagccaatc tcattcgcac gaaaatctgc tagttgattt
    19381 ccttttaatg tggtcacatc tttttgattg attcggatat gcccatctgt tggtttatct
    19441 aatgtggata aaatattcaa aagtgtcgac ttaccagaac cagaagcgcc catgatccca
    19501 ataaattcgc ctttttctac atcgaagctg atgccgctta atgcttgcgt ctttttctca
    19561 ttgttttttc cgtatacttt tgttacattc tgtacttcga ctattttgtt catttctttc
    19621 cctacccttt aattagtttt tccagtgctg ttttcggcac gtcttttttt tcgacttctt
    19681 catacgaagt gacaccttta tttttgttcc aggtgatttt taaatatgct cctttacgaa
    19741 gcggtcgagt caattgtccc ttgaagtcca gttctttttc cttaccctct ttcgtatagc
    19801 caggcaggat atatctatag ttgactgttt cttgtccaga gtctgatttt gaaactgttt
    19861 ttgtaccatc tgtcgtaatt tgtacatagt attcttctcc acccataact acgtaatctg
    19921 cgattttcac ccctgcaagt accacaacga tgaaaacaag tagaccgatc agaagttttt
    19981 tcactttttc cccttctctc ttactttata tactgattat agaaaaaagc gaacaattct
    20041 gccttcgaat caactatcat tcttctttca attttgtcat tttctttaaa acaactaaaa
    20101 aaagagttca agacaataat ccgtcctaaa ctccttcttc ctatcttaca aaaagtatgt
    20161 cacttacggt ctttcatgtc gcctgtattg ataacaggtt gcgttccttt gtctgtaatg
    20221 attgagacaa gtaaattatt gatcaattgg actttccgat catcggtgaa attgatttct
    20281 tgtccatctt cgatttgctc taaggccatt tgagtgattg tgacagctcc ttcaacaatg
    20341 gtttgtcgag cagacaaaat ggcttttgct tgttgacgtt ggagcatggc actagcgatt
    20401 tctgttgcat aggctaaatg gttcaagcgt gtttcgatta cttcgacacc tgctactgct
    20461 aaacgctctt gcagctcttt ttgcaactcg tcagacactg ccgtcgtgtt tcctcgaagc
    20521 gtcaaatcat tctcattgaa tgtatcatac gggtattggc tagcgatatg ccgaatggct
    20581 gtctcacttt ggatttctac aaaatcttga taatacgcta catcgaataa tgcctttgct
    20641 gtatcaacga ctttaaagac tactactgct gagatttcga tggggtttcc atccaaatca
    20701 ttgactttta aaacagaact attgaaattc cgaactttca acgaaacagt cattttttgt
    20761 gccaaaggta tcgtaaggaa aaagccattt tctcgaatcg tacctaaata ttgtccaaaa
    20821 aacaaaatta cttttgcttg attaggactg actacagttg ccgaacttaa caacaataac
    20881 gtgatcaacc aaagaaaaat acttaaaaaa agtgcccaaa ctttcgcttg ccacatacct
    20941 aaatagaaga aaaacaatcc aactaaagct aatacgacca atccgacaat ccctgcataa
    21001 ccattgatat aaaatgctct tttttctctc atgctacttc ctgcgctcct ttatccttca
    21061 ataaatatct aatgttactt ttttaatttt ttttcgaata cgcgccatta caccttctag
    21121 ttctaactct ttgtcttcag tgatgtacca accccttgcc tgtgtccctc cggctgcctg
    21181 cggtaaataa tccaattttg ttcgaaccca gtgcttttct tctgtaaaat agtcttctac
    21241 caagacctct agatccatcc ctgctgaaag aggcaaatct tcccgaacag atttaaagat
    21301 aaatcgttcc ttcttcccat catacttcaa tattcctgta tcgataatcg gtaaagaata
    21361 ataatccagc tctttcttca ccgtcaataa atgtttgaaa acttcatggt acacttgctc
    21421 taattggcga ccttctgaat tgttcgggta attaaccatc actttttctt tgtctggatc
    21481 atagaaggtt tggcttagtt gagtgatata agtatgtaat ctttccattt ctgtttttaa
    21541 cttttctctt tgaccttctt ctaccttcat atactctttc accctgtttt ctttttagat
    21601 attttctttc ttattttact atagttatgt gcgttaagcg atgaaaaaca gcaaagattg
    21661 gaaggatctt tgctgttttt tcttaaaata tccagttgag tacaggatca atctgtttca
    21721 ttgtcctctt gatccgtcgt aatattgatc gatgtgtctt tagcagtgat ctttagcttt
    21781 ttctgtcctt gttctaacaa agtatttgtt attggctggt agtctttttc aatataagat
    21841 tcaccagacg tttggatagt atagccactg ttttccatct catgcaaaga taaattgcca
    21901 ccgtttagga tgacttttcc ttctttgctg atcaaactgt cttctagatg gaagttgctg
    21961 ttttcacctg tgacgatcaa gttatccaca ttggcctcat ctaatcgaag tgagcctaat
    22021 tcatctttcg cagttattgt tcctcctgta aattcattga acgataccca gccatcatgt
    22081 gtcttagtat tcactttttt gattgatact tcgttgatat caattgcact attattccct
    22141 tcgattgtca attcagataa ttcatgatct gcagggacaa caatagtgat ttgtgcactt
    22201 gtgtcttcta aactaatgat tgcacctgtt gattttttga ctgcattcac agtcaacacg
    22261 ccatttttct cctttatttc tggcttttct gtcttatcat aagtcgtttt tatctcaaaa
    22321 tccaatccgc gtatgaccga aactgtttga ttttctgcat ggatgacgat tttgtttgca
    22381 tccttgattt tttttgtctc agtaaccata tcgatcaacc gaggaccatt atcccaagtc
    22441 agactctttt gagcgcctaa tgtataagtt gcacctgcta ggaggccacc agtaagcatc
    22501 agaccaatac cggtaatcaa aaaatgtttc aatctcaatt tatgtgtcct ccttcaaacg
    22561 cccgctgaat gcagatctct ttttgtaata aaacgtcgtc ccatccattt aacaaagttc
    22621 atcatgacat aaaaaagccc ttttgttcct ttcagcaaca gtggcgaaat aaatactgcg
    22681 cttcctgtgg cgaacagacc gacaccgata aagaataatc cactcagaat cgattgtcca
    22741 aacacactga ttcctgagat gatcatgaat aggccaacac ctaagatact gatacataaa
    22801 acatagatac taaaaatgag cgctagtaaa gcaaccaata gtcccccaat aaccaatata
    22861 aaccccagtg ctagtggaat gaggacagga gaagcaaata aaccgataat gattaaccaa
    22921 atcaggcgaa tttgtctttt tggatttttc actaactcgt tttctgcttg ttcttcttct
    22981 gaaaagtaac tgatttttaa ttttaaggca aactgctttg gtgtaccata ttcatcaatg
    23041 atctgatcta atgtttttcc agaatctaaa aaatattcct cataatactg gatcaaatca
    23101 aaacgatcgt cttcaggaat ttcttgaaga tgatccacaa cttcattcaa atattcattc
    23161 atttgtgctc tcctccaata accggtctaa agaatctcga aaaacaatcc attcatcaat
    23221 aatttcttct aaatgacgac ttcccattgg tgtcaacgag taatatcgac ggtttctgcc
    23281 ttcaaaaggt tcatcatagg tctcaagata tccttctttt ttcaatcttc tcaaaaccgg
    23341 atatagtgta gattcactga cagagagact ttcttgtacg atttttgtta aggcataccc
    23401 atacatatct tccttgctta atgctgctag cacagtacca tcaagaagaa cagttggaat
    23461 ctggatgtgc attacgtcac cttctattct caagtgtaat attttatttc aatctatatt
    23521 atacaacata taatatagtt tcaaaatcag tttgtcaaat acaaaaaaac tgactaaaag
    23581 atttgatcct ttttagtcag caaaaaatgg cttctctttg atttttatta taacagtggc
    23641 ataatccaat ttgtccacat ccagctcagt ggcatgataa cgatcattgc aggtatcatg
    23701 tcagctactg ggaacatttt cacgttgatc attctaaaac cggtagcgac catcaagaat
    23761 ccgccgcaag ctttaaaatc taagatcatg tctggtgtag tcaatggata aataaatttt
    23821 gccaataaga acaaaatata aaaaataatg aattggggaa ttgctatcac agaaacgaca
    23881 taccctaaat tacacgcaaa aatagctgct gtgaaaaaat caaggacaga tttagaaatc
    23941 aaaatcgtac tgtctcctgt catcccagaa tctaaacttc catagatccc cgtaccactt
    24001 gcacaaaata gaacaatgac cgtcactaat gtgctgataa attcatcgtg ggtcatacca
    24061 agcttttcac tagggaagat tttagaaata ggtacttgca tcagcgcagc ccccttattt
    24121 atcccattac ctaaatgaac aatcagacca atcgctgttc ctatcactaa agcaaaaata
    24181 acagccggca tatttttcat cggcgcaatg gaatagattc ccatccccat cgaacacaca
    24241 ccaaaaacca tcgtcagctg tgctttaaaa tgttcactta atttatttcc tactaatccg
    24301 ccaattagtc cgccaagcaa aacggaacct acattgatca aaactccagt tggcatcaaa
    24361 aaactcctct ctttttctac gaagcgaaaa aaatcagaaa aatttctgta attattgctt
    24421 tcgctgcaag aaagattgta acagacagag aagattacac caattcattt tttgtgttaa
    24481 agtattcttt attggaataa gtaggaggaa tatgatggat atccataaat tcgaagtttt
    24541 tcttgattta gccgaaacaa tgaatttcac caaaactgct gatcgacaat tcaccaccca
    24601 aggaaatatt tctaaacaga ttctttcgtt agaaacagaa ttagacgtca aattattcga
    24661 acgcgcccat agaaaaatcg aattgaccga agctggaagc ttattgctcc catatgtaaa
    24721 aaatgtcgta gaacaatacc atgctatgca agaagtgctt gaaacctata cgaaagacaa
    24781 caatctttca ttaaatatct taacgatacc gacaatgatc aattataaag gtttttccaa
    24841 aatcaccgag tttttgaaac gccatcctga atttacagtc cagttgaaag aagtggaaag
    24901 tgtcgaatta acttacaatg acgccaatga acatagtgac acgatccact ttgctagaaa
    24961 tttccaggcc ccaccaagtt cgattgagtg gttgcctact gaaacagacg attttgtagc
    25021 agttctacct aaaaatcatc ctttagctag taaaaagaaa ttggatctaa gcgaactgaa
    25081 aaaggagaac tttttgctcc ttggccccac tacgaacttg tatcaacctg tactagatct
    25141 gtgtcgcgaa gctggatttg aaccaaaaat ctcttataaa ggagcaagaa tcgacttgat
    25201 catcaatatg attgcaaatg acatggggat tgctattgtt atggaaaaaa cagtcaagaa
    25261 tctattaaaa gaaaatactg tgattctccc tatttctcca acaacagaga gttatcttgc
    25321 atttactcgt aaagtcggtg agcattcttt ggcttcagat acattttggg agtatttaaa
    25381 tcaagaagaa aactaagccc tacattttct gtccatctat ccacccaaaa aaagagagcg
    25441 acgcatgact ttcgctctct ttcttgcatc atgtccttgt ttcaatgaca cctctaacat
    25501 ttctcgtagt tcaataagaa agcagcgatt tttatgccaa aagaaccgta agaacatttc
    25561 cctaatcaca aaaaaacgac taaaaacccc taaaaacctt caatagaatt tttaggggtt
    25621 ttattctttt tcctattgtt tacttaaccg tatcattgct gtcgttccta ctccttcgat
    25681 actgctataa tcaatacgtc catcatgcag ttctagaatt tttttaacca gtgaaagtcc
    25741 tagcccattc ccagaaattt gacgacttgt atctccttga taaaattttt caaacattcg
    25801 atctctggtc tcttcattca ttcctacacc tgaatcggtc acttccagtc caatctctgt
    25861 ggctgtctca aatagtttga tatgaatctg tccattgatt tgattgtact ttatggcgtt
    25921 atccatgata ttcagccaca cttggtacaa aaactcttca ttgcctgtgt aatttactgc
    25981 agcaagttcg atatccaatt ctagctgttc tttctcccat ttaggctgca aaaaaacgat
    26041 tacttctctg atttgttcat ctaatcgata ctctttcttt tccagttgga tacgctggtt
    26101 ttccagtttg ttcaatttta agatgttctc tgttaattgt gaaagctgcg tgatactttc
    26161 gataatccga tataaaaata tttggcgttc ttcgtcagag agatttgggg cttgtaacaa
    26221 ttgaacatat ccttggattg ttgctagtgg tgttttgaat tcatgggata cactggacac
    26281 aaagtcattc ctcagtgtct cgatactatt gagttcttgg accataacgt tgaaatcttt
    26341 atacagttgc tgtacttctg ctactttctg ctgttcatcc attcgaatcg aaaaatctcc
    26401 cgttgctacc agactcatat ttgtccctag cgcactgatt ggatgcagga tacgccgccc
    26461 aacaaaaatc gatattcctg tacccacgac catactgaac cccgcaaaga cgaataacgg
    26521 gtataacgtt ggttttccag ccgattcagc aggcgagata gaatgtcgag acaataagaa
    26581 aataaaaccg agaaaacaca gcagtgtcac taagatcgaa atgaagacaa tactagcaaa
    26641 atagatccat aattgtgaaa atagcttttt tttcatcttt aaataaccgc cttatagcct
    26701 agtccccgta ctgtgatgat ttgaaaatct tccgtctttt caaaacgggt ccttaaacgt
    26761 ttgatatgta catcgatcgt tcgttcttct gtattcgtat ctaatcccca aatatcatcc
    26821 atcaactgtt ggcgagtgaa gatcttgttg ggataagaca gtaatttata cagaagcaga
    26881 aattcttttt gaggcagaat caacgaatgc tgatcttgta acactgtata agcatctagc
    26941 tctaattttg tttgtccgat tatcaagcta ctgctgtaat tgatctttga acgtcgaagc
    27001 aatgcttcca cacgtaggat catctcattg acatcgatgg gttttgtcat ataatcatct
    27061 gctcctaatt tgaacccgcg tttcttatcc tcaaaatcgg cctttgccgt aatcattaga
    27121 acaggtgtgt caaatctggt gctacgtaat gagtctagca agtcatagcc atccattttc
    27181 ggcatcatga tatctgtaat gattaattct atacgtgtcg cttctagttt tttcagcgca
    27241 tcctctccat tcaatgcggt gatgactgaa aagcccgctc gttccaacac agaacggtaa
    27301 agcaactgta attgcggatc atcttctact actaaaatac gattcattga tctccctctg
    27361 ttttttgaaa aatttctaga ttttttcttg tagttcatct tcagttaaca tttatttcgt
    27421 atactacgat cattgttctg atatacttat tagtataccg aatatttcaa tacgggagaa
    27481 gggtgattat gcttcaatta aaagatataa aaaaatatta taaagtcggc gaaaccacca
    27541 ccaaagcatt ggatggtgtg tctgtcgctt tcagacaaaa agaattcgta gcgattttag
    27601 gtcctagtgg ttctggtaaa acgactatgc tgaatgtgat tggtggacta gataattatg
    27661 attccggtga tatggtaatc aacggaaagt ctactaaaga ctttaaggac agtgactggg
    27721 atgcctaccg aaacaactct atcggtttca tttttcaaag ttacaacttg attggtcatc
    27781 taggcattat tgaaaacgtt gaacttggga tgacattaag tggtgtttct aaagacgaaa
    27841 aacgaaaacg ggcagaagat gcattgcacc gagtcggctt gacagatcat atgcataaaa
    27901 agccaaatca gttatctggt ggacaaatgc agcgagtggc catcgctcga gcactagcca
    27961 atgacccaga tatccttttg tgcgatgaac caaccggagc gttagataca gaaacaagta
    28021 tccagatcat ggaattgatc caagagttat cgaaagaaaa attagtgatc atggtcacac
    28081 ataatcctga actggcccat caatacgcag atcggatcat tgaattttct gatggtaaga
    28141 tcttaacaga ttcccatcct cacatcgaac gaccaaaaga tgatcaattt aacttacgca
    28201 gaacaaagat gagtttttgg acagcgttaa agctttcctt caacaatatc cgaacaaaaa
    28261 aaggacgtac tttcttgacc tcctttgctt ccagtattgg gatcatcgga atcgcgatcg
    28321 ttctgtccct ttcttctgga ttccaaaaac aaatcgataa cacgcaagct gaaacgatgg
    28381 cgaaatttcc gattactatt tctaaggtaa cgactaacca gactcgtgac gatgccggat
    28441 tgggtgcaag caaagcggat tatccagaca gcaaaacgat tactgcaaaa gtcagcgaag
    28501 aagataaagc acagcacacg aataagattg atcaaacata cgtcgattac gtaacagaca
    28561 tcgatcctaa cttaagtaat aatatcggat ttacccgtac tacaggaatc aatcttttac
    28621 gtgatgtaaa tggcaaagta cagcctgtca gcttctcgaa tcaaaatcct gatacggaat
    28681 ctctttcttt atctagtacg atgtcagcaa tgacaggtgt gggagtgtca agtttcccta
    28741 cacagcttga cactagcaaa gaaaactttt taaaagacaa ttattcttta cttgccggaa
    28801 gttatccagc ctcagcaaca gatgtcgtgc tgatcgtaga cggcaataat aatactaata
    28861 tcaacgcatt gaagaatctt ggatttgatg tgaaagaaga cgaaaaactt gattttgatg
    28921 atatcgttgg tacaacattc aaattagtaa acaacaatac ttactataca aaacttccaa
    28981 ctgggaattt tatccctaat acagattatg atgcgatgta tcagaatgct tctgatgaat
    29041 tgaagatctc ggggatccta cgcgtgaaat cttcttctac aatgaatctc ttatctccag
    29101 gaatcgctta tagcgatcaa ttaacgactc aaatcgtcaa cgaaaacaaa gaatcagaaa
    29161 tcgtcaaagc tcaaagagac agtggtgtaa atgtcttaac tactgaaaaa gttgacgaaa
    29221 atgccaaaca aacattgctt agttatttag gtggagacag cttgccttca agtatcatga
    29281 tttatccgaa caattttgaa gacaaagaaa agatcttgga ttatctagat gattacaata
    29341 aaggcaaatc agatgaagat aaaatcattt atactgattt agctggaacg atgactgaac
    29401 tgactggcgg attgatggat gctatcactt atgtccttat tgcttttgca gggatctctt
    29461 tagtaacaag tatgatcatg atcagtatca ttacttatac atctgtgatt gaacggacaa
    29521 aagaaatcgg tgtactaaaa gctttaggtg cacgtaaaaa agacatcact cgcgtatttg
    29581 acgcagaaac gtgtatttta ggtatttctt ctgggatatt aggtgtgttt attgcatggc
    29641 tagctacgtt ccctatcaat agcatcttat acaacatgac ggatctaaaa aatgttgctc
    29701 aactaaatcc tgtccatgcg attattctag tgatcgtctc tactatattg acgatgcttg
    29761 gcggtcatct tcctgcacga atggcagcga aaaaagacgc cgctatcgct ttgcgtgcag
    29821 aataaggttg tgacaaccac atttagattt gagtcataag agcgctgaaa tagtagaaaa
    29881 cagcaagaaa cgaggaagta atatgttcct tgtttcgcgc tgtttttttt aattattttt
    29941 tataattctg gtatactttt ctttcctttg taccacaact cttctggcgt atacctgtct
    30001 tttccattta tgaatactgt tccatctgct tcaatcgtta cttcagtccc aatccacata
    30061 ttacgagaaa gtgcgagaag attttttctt tttcctcttc ctttttttcc aaattgcaag
    30121 gcatcggtta tctcttctgt ccaatgagat ttcacgtatt ttacttctcc gttttcaaat
    30181 gtaactttca aaatattcgc tatattttcc acttttactg ctttaggaaa tgactccttc
    30241 atttttttca ctttccttcc tccaacacta agtatgcgct ttcttttagt gataagtata
    30301 tcatagttta ttctattatc aaaaaaacgc atccattcca ttgtagaata ctttaccgaa
    30361 caagttgaat tgttttcctc tttttcacct tttactatag ataggtaaag acaactaagg
    30421 aggaatctct catgacagat caaccgtatg atttaagaaa agtcatcgaa gaactaaaaa
    30481 cacttccagg tcaatatcac gaaacagata ttgaaatcga tcctgaagca gaattatctg
    30541 gtgtttaccg ttatatcggt gctggcggta cagtaaaacg tccgactcaa gaaggaccag
    30601 ctatgacttt caacaatatc aaaggtttcc ccggcacacg tgtgaacatc ggaacaatgg
    30661 ctagtcgtaa acgtgtaggt gaaattttgc accatgatta tcgaacactt ggtcgtttct
    30721 tgaaagacgc tgtagaaaat ccaatcaaac ctgtgaaagt agacaaagca gacgctccag
    30781 cacaagaagt cgttcatcta gctaccgatg cagacttcga tattcgcaac attttgcctg
    30841 cacctacaaa tactcctgaa gatgctggtc catacatcac aatgggtgta gtcttaggat
    30901 cagatccaga aaaaacaatg agcgatgtaa cgatccatcg tatggttcta gaagataaag
    30961 atacgatcgg tatgtacatc atgcctggcg gacgccacat tggtcacttc caaaaacaat
    31021 tcgaagcctt gaataaacca atgcctatca ctatcaatat tggtttagat ccagcaattg
    31081 ccattggcac gactttcgaa ccacctacca ctccacttgg ctacaatgaa ctatggtgtg
    31141 caggagcttt gcgtaacgaa cctattcaaa tcgttgatgg tatagcagtc gatgaagtgg
    31201 gtattgctcg tgctgaattc atcattgaag cagaaatcat gccaaatgaa acaatgcaag
    31261 aagatatcaa cacgaactca ggaaaagcaa tgccagaatt tcctggctat actggggatg
    31321 ctaatccagc tgtacaagta gtgaaagtca aagcagttac tcaccgaaaa gacaatccaa
    31381 tcatgcaaac aacgattggt cccagtgaag aacacgtatc tatggccggt attcctacag
    31441 aagcaagtat tcttgccttg gtcgataaag ctattccagg aaaagtagta aatgtctaca
    31501 atcctcctgc aggtggtggt aaattgatga ccatcatgca aatccgtaaa gaaaacgaag
    31561 cggatgaagg tattcagcgt caagcagcta ttcttgcttt atcagccttc aaagaattga
    31621 agacagtcat tttagtcgat gaagatgttg atattttcga catgaacgat gtagtttgga
    31681 ctttcaacac tcgtttccaa ggagataaag atatcatcgt gatcccagga atgaggaacc
    31741 atccattgga cccatcagaa ttaccagaat ataatcctgg ctctattcgt ttccgtggga
    31801 tgagtgcaaa aactatcttt gacggtacag ttcctttcga tatgaaagaa cattttgtac
    31861 gtgcaaaatt ccaagaagta gaagattggg aaaaatattt gaaataatga atgatctgca
    31921 actgttttga catcataaaa aggagtgcgg aaaatggaac aattggcaca acggaagaaa
    31981 aaaatcgtga ttggtgtaag tggcgcttca ggcaccattt atgccatcaa cctagtgaag
    32041 aaactaaaag aatatccgat gatagaaaca cacggaatca tcagtccatg ggcaaaacag
    32101 aacttaaagc tggaatccga cttatcacta actgaattcg aaagcctgtt tgattaccat
    32161 tattcaaaca aagatttagg tgctgctgtc gccagcggat catttttgac cgatggaatg
    32221 atcatcgtac cggcaagtat gaaaacagtc gcaggtatct ctgttggttt aggcgacaac
    32281 ctgattgcac gtgctgctga tgtaacaatg aaggagcaac gaaagttagt cattgttcct
    32341 cgtgaaacgc cattaaatgc cattcacttg gaaaacttga ctaaattagc acgactaggt
    32401 gtgcaaatca taccaccaat tcctgctttt tataatcagc ctcaaacgat ccaagatctt
    32461 gttaatcacc atacaatgaa acttttggat caatttggaa ttgtaaatga gcattcaaaa
    32521 cgttggcagg gtgattaata tggaaaaaga aacgatagtt gaagtaacca aactaggttt
    32581 cacgatccaa gctgctgtta aagtaatcgg taaagactta ttgatcactt taacaggcgg
    32641 agatacacca catatcggaa cagtcactac attttctcgt gatacagaga tgcaaactgt
    32701 acgtttccct agccacgatg gtcggttcca taaagacgat gtcttagccg aaaaaatagc
    32761 tgtgatcatc aaaactgccg ttccgggaag ttgtacgatc acttccggta tccatgttga
    32821 ccatatctcc aatgcacaaa tcaacgcctc ttttccgatg tcagaagagt taggttacaa
    32881 gattttagat tggttggaaa aaatagattt ccatatcaaa gaaccagttt attataaaga
    32941 tggtgaaaaa acattatgaa agaagaggtt gtgaaatcag cgttatgacc tgagtatgag
    33001 agaaaaaaat ggaaaaatag cttctcatat tttgccgttt ttgagctaat accgagggac
    33061 agctgattga acgccgttta tcaagaggtt gtgataaagc cgcttcgttt cgagtagtaa
    33121 gaaaaatttt gtaaacatgg gtttccacat ttttgcataa atttgactta ctaccgagaa
    33181 actggctttt gaacaccgtt tatcaagagg ttgtgacacg tcttttgtca caacctcttt
    33241 tatttttgct tcactgtaac tgctacaata tcaccgattt cttttccgat gtttttacga
    33301 atatcttttc gtagtccaag gatgtagcag actgaaccat ctggattttt cacgcccata
    33361 ttcacgatac tgccttcata cggcatattg tcaaaagttg cttggacttt aactcttcca
    33421 gcaccgaatt cttttcttat atcatacgga aaaataatat atgccccgcc cttgtcgact
    33481 tcggaagcat agatttttga ttcaaaagaa taaatttccc tcattttatt tctcctattt
    33541 tttacatcta gtatagcata gcaaaaaaaa gaaggaactg tgacaaaagt cttgtcgcag
    33601 ttcctttttt cgaataaacg gtgttctcct gccaactcct cggtcacaag tcacaatagt
    33661 caacaaacat gagaagctgt tttttgaaat tgctccttgt gactcagagt taaacggcag
    33721 gctcacaacc tcttccttaa acggtgttca aaagcttgca tctacggcac taagcccaat
    33781 cagatgaaaa atatggagtg ctatttttat ctgattgttc ttactgcttg atgcagagca
    33841 gctttttcac aacctcctac atggtgttca aaatattatt cgccttttgc ttctttcact
    33901 aattttgcga atgcttgttg ttggaattca gcagctaagg ctttcgtacg ttgttcttgc
    33961 tctgctttgc tcatttttgg attttcagtc attgctttta ccataccgtc ttgaacataa
    34021 gcttttgctt cactttccat cgtactttgc tgtttcttca ctaattcagc taacttggct
    34081 gcttggtccg tacttaattc tacccatgtc tctgggattt tgaatgtatt cactcggctg
    34141 tcatattcgt gattgttatc agcaatattg aaccagaatt tatacatgtc gtttttgtat
    34201 tcccaataag tagttttcgt cactttttca gcatcgccgt ctgtcttttc tactttattt
    34261 gtttcgttat ccgtaccagt tgcttttggt ttttcttgtt tttcatttgt tcggtaaaga
    34321 tacacttttt cttttccatt ccctaaagct ttatacaaaa gcatatctgc tccttgggaa
    34381 tcagcactgg aaacaatttt ttctgttttt gtttccgtta ctttttccat tccaaagtga
    34441 ttggacaaat tagcgacgat caaacttagt gaagcgacga agattagtcc gaaaatcaaa
    34501 gataaaaagg tttgccatgt ttttttagcg aacacgttcg ttaaagcaaa agcaagcaag
    34561 ctgacaatca aaactccaat aatcatgcat tatcccccgc tttcattaga acaggttctt
    34621 ttttcttcaa taaaaaggca ataataaagc caagaatccc aaagatgatc gctacgataa
    34681 acgcggcatg gtaacctgac agtgtggcat tgattgcctg atctttataa gctaatggcg
    34741 tattatccaa tacagatttg cttgggatat ttgcttttgt cacattcgtc aacacactga
    34801 tcaaaatagc tgtaccaatc gagcttgcga tttgacggaa tgtattattt accgctgtcc
    34861 catgactgat caagttattt ggcaatgcat tcataccaga agtcgttacc ggcatcatga
    34921 ccatggagat cccaaacata cggatcgcat aaaggatcac gatatataaa actggagtcg
    34981 tttttgtcaa gaaagcaaat ggtgcagttg ccgctgtcaa aatgaacatc cctgtgatgg
    35041 ctaaacgttt cgcgccgtat ttatcgaaaa tcgcacccgt cacaggcatc atagccccca
    35101 tgatcaatgc acccggcaac aacatcaagc cagaatggaa tgctgattct ccacgaatat
    35161 tttgaatata caatggtacg accatttctg cccctaccat cgccatattt gttacaccag
    35221 ctaaaacagc tgcaatcgtg aatgtagatg atttgaatac acgaagttcc aagaatggat
    35281 gttccaaatg taattgacgc catgagaaca aggcgatgaa aatcacacca acaactaaga
    35341 aaccaattac tttcaagctt ccccagccgt catctcctac actagaaaat ccgtaaagta
    35401 aactaccgaa tccaatcgtt gataaaatag cggataaaat atcaatgcta ggatttgaca
    35461 atggaataac acttttcatt aggaagaatg ctaaaaccaa gacaagaaca acgattggca
    35521 gtaccatacc aaacaagtca cgccaatggt agttatcaat gatataacca gataatgtag
    35581 ggcctaaagc aggagcaagt ccaataacga tccctgacat ccccattgct gatcctcttt
    35641 tttctggcgg gaaaatagat agcatgatat tttgcaataa tggcatcgat actcccacgc
    35701 cgcaagcttg gatcaagcga ccgattagta atgtactaaa attaggtgct gtaaagcaag
    35761 taatcgttcc gatcaaaaag atcgtcatgg cagaaagata taaatttctt gaactgaatt
    35821 tattgatcag ccatgctgtt attgggatca tgatcccgtt gactagcaag aaaccagtcg
    35881 ttaaccattg cacatcagac gctgaaatat cgaaagcttt catcaatgta ggaaaagctg
    35941 tcgtcaataa ggtctgattc aaaacggtac aaaatgtacc aattaataag acaacaacta
    36001 aaagtgtccg attatatggt tttccgtaaa tatctacggg ttggttgtta ctcatatttc
    36061 tccttctttc tgaatttttt ctgaaactac tagaatactt tagtcctaaa ttcgctattt
    36121 gtcaacctac ttgacacata agttaatacg gatataatta agggaagcaa gaggacaaaa
    36181 ggagtgacaa aaatggttgg ggcttccctt aaacgtttgt tggaaaatga tcaattggtt
    36241 gttggcatca gtgaattaag tgatatcgta ggtgtgtctc cacgtcaatt aaggtattgg
    36301 gaacaaaagg gattcattcg ttcgatcgaa gctgatgcga attgtcctag aaaatatcgt
    36361 ttacctacag tcataaaagt agaactgatc aaaaaatatt tagatgacgg atttactttg
    36421 tcaaaagctg cagaaaaggc agaaatccgc caaaagaaaa tgcaccatgt ccgtaaagtt
    36481 ttttcaaaag caatcaaaga tatcgaattg atcgacgaac gctatacgat tttacaaatc
    36541 ggtaacttca aagaagagaa tgctaagatg tacatcattc atgacaacga gacagacgaa
    36601 ttaacttaca agctccttcc tgtcgatgaa aaagctgact acgaacagtt gttaaaagaa
    36661 ctctaaacgc cactaatctc ctaagtacca agctttttaa taagtagatg ctacataaat
    36721 acttgaaaaa aggcctgttt gaacataaga atatccgaac ggtgatctgc gtttctttca
    36781 tatagaaagg aattctccat cactgttcgg atattttttt atttttttcg gttactttct
    36841 tctaatacat ctgttagcca atttgcatat tctgtttccc gattgatcgc atgcgctaaa
    36901 atcaggtagt gtccgtaatt ttcatgtttt tctttttcag taggaaagat cgtttttttt
    36961 cgctctaata gatgaagtcg tttttcttca tgaaggtcac gctgctgctg aataatttct
    37021 actaatgccg gatcattcac gtctttgacg aaatacaatt tcagaacaaa ttcgtctcga
    37081 ttaaccggta gttcattggt tggtgtatga atccactctg ccaattgttc cttcccttct
    37141 tctgttaatt cataaacttt cttttccaat ttttcacctg tgatctctac ttgatgctga
    37201 atgattcctt gctcttccat ttttgcaagt tcaggataaa tctgactatg ttttgcttgc
    37261 caaaattctc ctatttcatt tttgaacgat tgattcaatt catatcctgt catcttcttc
    37321 ttacttaata accctaacaa aatatatcga agtgtatttt ttcttgccat cagttttccc
    37381 tccttgacaa atctttaaat ccattctatc ataaatgagt aatcgaaaac atgtagttta
    37441 ttacatgttt taatttataa ggagatgctt aaacatgaaa gaatttaaag aattggaaga
    37501 ttttattgga actcacttta tctatacgta tgacaatggt tgggaatatg aatggtatgc
    37561 caaaaatgac catactgttg attaccgtat ccatggcgga atggtcaaag gccgttgggt
    37621 aaaagaccaa gaagctgata tcgttaaatt gacggacggc gtattcaaaa tcacttggac
    37681 agagccaaca gggacagacg tggcattgga ctttatgcca aatgaaaaga aacttcacgg
    37741 aatgatcttt ttccctaaat gggttgaaga acatccagaa atcactgtta ctttccaaaa
    37801 cgaacatatc gcagaaatgg aagcagctcg tgaaaaatat gaaacttatc caaaattact
    37861 tgttcctgaa tttgctacga tcacttatat gggtgatgca ggtatagaca atgaagatgt
    37921 tattagcgaa gaaccatacg atggaatgcc agatgatatc cgtagcggaa aatacttcga
    37981 tgaaaattat aaacgagtaa aataaggttg tgaaagagag gttgtgaaat cagcgttctg
    38041 acctgagtat tagagaaaga aacggaaaaa tagttcctca tgttttgccg ttttttgagc
    38101 taataccgag ggtcagctga ttgaacaccg tttatcaaga ggttgtgaaa tcagcgttat
    38161 gctttgagta ataagaacgc tagttagaaa atagcttttc atattttcta ggctagtggg
    38221 cttattaccg agaagcagcc gtttgaacac cgtttatcaa gaggttgtga aatcagcgtt
    38281 ttgacctgag tattagagaa acaaatggaa aaatagttcc tcatattttg ccgttttttg
    38341 agctaatacc gagggtcagc tgattgaaca ccgtttatca aagattgtaa caactgcata
    38401 ttcacacagt taaattagtg acttcaaaat gaaataatgc tcccagcaaa aaattaggct
    38461 gggagcatta tttcgttatt ctgttcatca aaaatcagcg ttttgattcc atttcatcac
    38521 aagttcctcc tcatttactg cttcatcttt ttgctgtgtt tgaattgtaa aaccttgtga
    38581 aagatagaaa tgaatagctg attgattttt ttgatagact gtcaaagtca gtgttttatt
    38641 ggtttctttt atttttttca ttagttgcgt accgattctt tgttgccggt attccctttt
    38701 cacaaagatt cctgcgataa actgatcaat caccccagca aaaccaataa tttcttgatc
    38761 tatttctgct aagtaaatca ctgcatctgg taaagctttc ctaacagatt ctacttgatt
    38821 ctcccaataa gttgatggaa tgaattcatg agaatccaag ttagcatgca gccaaatcga
    38881 tagaacagat tccagttctt tggcagacaa ttttgttgtt gtgcggatca ttttttctct
    38941 ccttacttat tcattgattt taaaataaaa accatttttt tgaagctctt ttacaagttt
    39001 tcgcccctca aaaaagtata tcttctcttg ttttactttt ctaataccaa aagaaacagc
    39061 aactttagga tcatcagctg aaacgattcg ataagttcca ccaggcaatt gatccagctt
    39121 gactttccca tgatggtcgc tttgtactga aaacggcaca ccatttctag taagtgtttt
    39181 tttcttatgt ttcaaaatga atgaactacc atttaccggt ttccctgacg aatcactaat
    39241 aataaatgaa aaatcaagca cttttttact taatctatac tgatagatta gtcgtccgat
    39301 aaacactaac agtatgacta cagctattat cgttcctgca agaataaacc attgccgcat
    39361 ggttctttcc tgatttcctt ttccttcttc tttttttacc gattcgttat aaggaatccg
    39421 atgtcctgtc actagcaaac gatgcgtgtt gatcatatac ggcgtacatg taagcaaggt
    39481 aactaagtct tctccttctg aaatcgttag aacagacgtg tcatctggca gtaccacttg
    39541 tatattttta acttgataag ccaatttctc ccccaaaaca gtcaaaataa acgtgtctcc
    39601 gttttctaaa cgatctaatt cggtaaataa ccgtcgatta ggtaaaccgc tgtgagcaga
    39661 gatcactgaa tgcgtcgaat ttccaccaag cgggaaagag gttccttgca agacgcctgc
    39721 tccattttca aggatcctac cattgagccg atcataaagt gaaatattta attggatctt
    39781 aggtatattg atacttccga tcagccattc ctctaattgg ctggatgttg tactttcatt
    39841 tctgttcttt tcattgaaag gatcagtacc aggattgaat ccttggtcag ttaatcgttg
    39901 ttttttctcc tccattttct ttcgctgcac ttcaatttca gctgcggtcc gtttttgtac
    39961 ttgttcgata cgtttttgat caataaaact atttaaagaa tccacataaa atggatataa
    40021 agcgatcagt aaccctacga cgatacatat taataaaaac aaacgaaaca aaagtcgacg
    40081 agactgtttt tgcttactct gcttcacatg tgtcctccct tgcaagtttt tttgttcttt
    40141 cttttattat atgggttcag gaaaaaaata gaaaatgaaa gccgatgagg ttgtgaaaaa
    40201 gctgtcctgc atcaagtaat aagtacagcc caaaaaaata gctactcata ttttttgttg
    40261 ggttgggctt attaccgcag atgcaagctt ttgaacaccg tttaaggaag aggttgtgaa
    40321 aactgcgtta tgctttgagt aataagagcg ctagtcagaa aacagcttct catattttct
    40381 aggctagtga gcttattacc gagaagcagc tgattgaaca ccgtttaccg agaggtcatg
    40441 aaaaagctac tttgtttcga gtattaagaa aaattctgtt tcaaaacaag gagatgcaac
    40501 aaaacttatg tcgcatcctc tctccttaga actttcactt atgtttttct tgtaaaattc
    40561 ataaatattt tcatgaattt tctaatttct tgcctacctc tcgcatagga tatatgatga
    40621 atttatctaa agtttgcaaa ggagttcgac aattgaaaaa atatggccca tttttagcaa
    40681 tttttttcat tgctttggca tttctcatgc ctcatttatt cacacataaa ctaattttgg
    40741 gtgctgatgc cccattccat tacaatcggt tctatgatgc cgcgcaacaa attaaatatg
    40801 gggatttcca ttattttcct tccctttatg gattccagca aagtggcagg atcgtgaatg
    40861 ccgtttacgg cccatttttc gcttatttac aaggattgat cgtgttgctt gcaggtagct
    40921 ggtttcatta tcagattctt tcaaatttca ttctttatct tatttcagga ttttctatgc
    40981 tgattttact gagatatgtc aatgttcgaa attgggtaag tgtaccagta gccgttttat
    41041 tcatgagtac gtattctatt caatactggt ctatcaacca aggattcact agctggggga
    41101 cttgttttta tcctttatgt atgatcccta ttgctgattt cgtccttcat aaaaagttcc
    41161 ctgttatcaa agtcgctgtc tcagtgggac ttatgacaca gatccattta ctgtcttcga
    41221 tcatgttagc tttgatgtat atcccttttt atctttatta cttttttaac cagactgaaa
    41281 aaggaaaagc actgcttcaa ctgattaagt cggtcttgct ttattttgtt ctgacagcaa
    41341 atatttgggc aggattagct attgtttaca gtgggaataa gattcttcct ccctttgtta
    41401 acagagaaat gagtcaaaag gcgatagatc tggatggcag ttattggctc cattacccta
    41461 aggcctttcc tatattgata ttatcaggtt ttttgctttt tattttcttt caacaaaaga
    41521 acaccttatt ccaaaaaatc gtgttgtaca caagtcttgt tttctttttt ctttctacta
    41581 gcttgattcc atggacgaca ttgatcgaaa aaaatgtgcc ttttattagt ttgatccagt
    41641 ttccatttcg atttttcgtg ccatttacgc tattgcttct actttacctt gccctttcac
    41701 tgaaccagtg gtctgaacaa aagtggttcc gtcttttaag tatatttggc attcttattt
    41761 tttcagcaca gacgatccag aacttatacc agcatctgga aaagtgggaa aatgaaacat
    41821 ttgtcagtcg acatacttat ctattcgata cgccggaaga agcaaggaaa acattcttca
    41881 gcaaagatat gtctaccagc ttgttcgttt tccaaaaaac cacacctgac tatctgccaa
    41941 tctataagga gacaaaagac aataaatacg accggtataa cgaagaaata ctagaaaacg
    42001 aaacaacttt tataaaaaca catccaggcg gtcttttgac aatcaagtgg aaaaacactg
    42061 gggacaaaac tgttcatatc cctgttatcg tctacgatcg gactgtgcta cagcaaaatg
    42121 gaaaaacatt gactgattat gaagtaacag atatcgggac cccaatcgtg aagcagaaaa
    42181 aaggaatcaa cgagctcacg cttcattatc aaacgccaat ctatttctat ttcattcttt
    42241 ctcttacttt gattggctgg tttaccttgc tatgtttatt tatttatcat agatacaagc
    42301 ttttacgtgc ttaggcaaaa gggataaata catccgatca aaaaactctc caaacggtat
    42361 aatcgtttgg agagttttat tttaatcagt ttttttatgt tatatgttat attttttatt
    42421 cgttcacatc taattcgatt tccaccccgt agtgatcact gatgattgga ctatcgatcc
    42481 catcaaaaat ccgttgatat tttttaacct gcattccttc agggacgaaa ataaaatcta
    42541 tacgcaattt ttctgtattt tgttcccaac catctatttt cttttccaca gtagctgctt
    42601 ttcctttttc attcgcgaca acatacgaat cttgaagaag aaggcaactt tctcttacag
    42661 tataataacc ttgagagtct actggattat tgaaatcacc taggaaaaaa agtggtacgc
    42721 gagtttccaa aaagtagttt tccaagtttt tccactcaaa ataaaagcca ctttttttat
    42781 ctgtccacca agaaaagtgg cagctcgaga caaaaatgtc ctgatcatct atctttgttt
    42841 tcccaatcaa gatcttgcgt gtctgataat cttctttctt ttgagattgg gagacggtaa
    42901 ggacctgact ttccaatgtt tttttcgtaa gcagtgcatt tccttcttca tatttatcat
    42961 aaccgatgtg gctcatttcc caactccaaa aatactcttt tccgtgttcc tttaaaagct
    43021 gaactaaacg gtaggcaaaa ttatctttac ggataggtac ttgattcttt acctgacaga
    43081 acttcattag cttcgcaggt tctacttctt cagattccag taattggttg atttcttgta
    43141 aagcaatcac ttcataatct tctaacagga tcttatctgc taattcttgc aattttttct
    43201 ctggttctgg ctcaagccag ctatgcgtgt tgagtgtcat gaatttcatt ggttttctcc
    43261 ttgtcttcat ttcgaaacaa aagggttgtg acaaaatttt tgtcacaacc tctccagcaa
    43321 atagtgttct cctgctaact cctcagtaat aagctcaatg gaccaggagc tattttttgt
    43381 ttggctgttt ttatcacttg gtgcagggca gctttttcac gacctctcca ctaaacagta
    43441 ttcaaactgc tgcttctcgg taataagccc aaccaaacga aaaataggag gagctatttt
    43501 tgtttggctg ttcttatgac ttgatgcagg acagcttttt cacaacctct ccttacattt
    43561 taatggagcc gatcacttta ccagctgtca ctgtacctaa ttgcttgatc actacttctt
    43621 ctactttatc gtttgtaaat acgatcatga tcgttggatc tttttcttcg ttttggatca
    43681 catcaagatc catctcagct aatttcgttc ctgttcctac ttcttggcct tctttgacag
    43741 caatctcaaa agctggctga ttcatttgga ctgtatcgat tcccatatgg atcaaaactt
    43801 cgataccatc tttcgtttcc agcccaattg catgtttgct agggaaaata cttgtcactt
    43861 ttccagcgat cggcgcaaca acttctcttg atgttggaat aacagcaaag ccgtcaccca
    43921 tcattttttg tgagaatacc ggatctttta cttcagataa aggaatcact tctccagaag
    43981 ctgcggaaac tagttcttgt tctataccta agtattgctt actacctgct tgtccatttc
    44041 ctgtagaaac catttctggc tctggaatct ctgctcctga ttgcagcaga tcttcgatat
    44101 ctgatttcaa gacatctgct tttggtccat aaactgcttg gactccattg tctttgacga
    44161 tcaatcccat cgcccctgct cgtttccagg cttcttcagt ccctactttt tctcgatcat
    44221 ttacacttac gcgtaaccga gtcatacaag catcgacatc tttgatattt tcttttcctc
    44281 ccagcaaatg aatgatctgg accacttgct gatctgctgt tcctgttccg ctgttagata
    44341 caacggtttc ttcactgtca ttgtcgtaat ttccgttacg tcccggtgta gcaaagttga
    44401 attttttgat caagaaattt gctaagaaat acgtaacaac accaaaagcg atcacacaca
    44461 acacgaagtt gataagatct ccacctagac ccgctttgat agctaacggt gtccgagtca
    44521 gcaattcgat attcccaaat gaatggaccc gcaatggcaa gatatctgcc atcgcaaatg
    44581 cagccccttg gatcactgaa taaatgacat ataatggcac agcagcaaac ataaacatga
    44641 actccaatgg ttctgttaca cctgttagaa agacagccaa cgctgctgaa aaatacattg
    44701 atttatatct tgctttctta tcaggatcga cattacgata catcgcaaaa gccattccca
    44761 tcaagatacc tgaagaacca atcatttgtc ctactttgaa acgagccggc gtccagttat
    44821 gcaacacaaa ctcatattgc gacatatccc ctgcaccttt aagattcact aaatcagtgg
    44881 cccaagccaa ccataacgga tcttgcccaa atacttgtgt ccccgcctgt gcgcctgata
    44941 aaatctcata cgatccgcca agctgtgtat agttgatagg aatcgtcagc atatgatgca
    45001 atccaaatgg caatagtaaa cgttctaatg ttccaaataa aaacggtgct agaatcggtg
    45061 cgctttcttg tgattctgca atccataaac caaaattatt gattccagcc tgtacgtttg
    45121 gccacaccac agcaagaatc aatgccacga ttgttgacca taagataaca acaaacggaa
    45181 caaagcgctt tccattgaaa aatgacaaag catccggcaa ttttctataa ttatagtact
    45241 tattataagc cattgccccg acaaatccag cgatgatccc tacgaatact cccatattca
    45301 aagctggtgc ttccaataca cttgtaaaaa atcctttaac catgatcttc gtaccgaaca
    45361 gagtgtgggt aaatgcatct tcattcgcaa gcatgtcact tgtgacacca aagatagccc
    45421 cagtgatccg attgattaaa ataaatgaga tcccagctgc aaatgctccg cctgctcttt
    45481 cttttgccca acttcctcct attgctaaag caaataaaat atgaagatta ccaataattg
    45541 cccaaccaat attttcaatt actccgcccg ttgtgacaag cggcgtccaa tcaggattga
    45601 tcaagggaat tgatttccct atactgatca tcaacccagc tgccggcata actgctacca
    45661 caaccattaa agcctttcca aatttttgcc aaaactcaaa actaaatatc tttttcattc
    45721 cgtttagctc ctttacgcaa tcggtttcgt taaacgtatt atagcgtgcg ttttcttttt
    45781 tgtaaatgct ttatttttta tttttctgtc tataccattc tataaaacct ataatacgtg
    45841 tgttcccttc tataattttc tcctaaaaat ttattttttt aagctttttt ctatgaaata
    45901 ttttcttttg tctattgact tgtgcaatcg gttgcgttta ttatgaacgt ataaaacagc
    45961 tcattaggag gagaaaaatg aaacagatca aacgactatt tgctattgat ccatggaaaa
    46021 tcagcacgac acaattagat aaaaaaaatc ttcgactgca agaatcttta acaagtattg
    46081 gaaacggtta tatggggatg cgcgggaact ttgaagaaac ttactcaggg gaccaccatc
    46141 aaggtactta tttggcgggt gtctggtatc ccgataagac acgtgtcggc tggtggaaaa
    46201 atggttatcc agaatacttt ggaaaagtga tcaacgcctt gaactttatc gcgatagata
    46261 tttcgatcaa cgatcagctg atcgacttgg cttctttgga accagaagac ttttactggg
    46321 aattagatat gaaaaatggt gtattatctc gtacattcac aatcactact tctactaata
    46381 aagtacgcat ttccttcgaa cgttttctga gcattgtcaa aaaagaatca gcttatatct
    46441 atttaactat agaaatgctt gaaggtagcg gaacagtcaa actcgtatca aaattggatg
    46501 gaaatgttca aaacgaagac agcaactatg atgaacattt ttggaatgag attgatcgtg
    46561 gaactataga agaaatcggc tatttgacga ctaaaactat tccaaacaac tttgggattg
    46621 aacaatttac tgttactgca gcaatgcgcc atttcgttaa tggaaaatca gtcatccctg
    46681 actatcacga agatacttta gcattacatg gttgcttgtc tgcagaatta tctcaaggtg
    46741 agacatggtc agtggaaaaa gaaattatcg ttcttactag tagagatatt ccagaagcgc
    46801 agcaagcaga aaaagctgtc tcagaattaa cgaaccagac aaacaattat atccaagcaa
    46861 aagaagagca aacagcggct tgggcagaac gatggaagct tgccgatgtg gtcatcgaag
    46921 gagacgaaga agcacaacaa ggtattcgat tcaatttgtt ccaactattc tctacttact
    46981 acggcgaaga tgaacgattg aatatcggcc ccaaaggatt cacaggagaa aaatacggcg
    47041 gagccactta ctgggataca gaagcgtatg ctgtccctct ttatcttgct ttagccaaac
    47101 ctgaagtaac taagaatctg ctgaaatatc ggcataatca actgccgcaa gcaatccaca
    47161 acgctcagca acaaggatta aaaggcgcgc tttatcctat ggtcaccttc actggtgtcg
    47221 aatgccataa cgagtgggaa atcacattcg aggagattca ccgaaatggc gcgattgcct
    47281 atgcaatcta caattatacg aattacaccg gcgatacgga atatttgaaa aatgaaggac
    47341 tggaagtttt agcagaaatc gcacgcttct gggcagaccg tgtccatttt tcaaaacgag
    47401 caaataaata catgatccac ggtgtgaccg gcccaaacga atatgagaac aatatcaata
    47461 acaattggta tacaaatacg atcgcagctt gggtcttgcg ttacacgcga gaaaattatt
    47521 taacttatca aaaagaagct gctatcacta tcagcgaaga agaactagcc aaatggaaag
    47581 atatcgcaga gaacatgtac ttcccagttg acgaagaatt aggaatcttt gtccagcatg
    47641 acacattctt agataaagat ctcatgcctg tatccgatat tcccacaagc gatcttcctc
    47701 tgaatcagca ttggtcttgg gacaaaatcc ttagatcttg cttcatcaag caggcagatg
    47761 tactacaggg gatttatttc ttcaatgata cttttacaaa agaagaaaag caaaaaaact
    47821 ttgatttcta tgaaccaatg actgtccatg agtcttccct ttcgccaagt atccattcga
    47881 ttttagcagc agaattagga atggaaagca aagctgtaga aatgtaccag cgaacagctc
    47941 gcttggattt ggataattac aataacgaca cagaagacgg cttgcacatc acttccatga
    48001 ctggtagctg gttgtcgatt gtccaaggat ttgctcaaat gaaaactgcc catgatcagt
    48061 tgagattcgc accatttctg ccaaaaactt ggaaagctta ttctttccat atcaattatc
    48121 gcggacgttt attgttcatc tcagtcacag atgaacaagt tactttcgag ttattagaag
    48181 gttttcctct agaaatttac gtctatgaac agtcttattc tttagcggat cgtctagaaa
    48241 taacgctaag aaaggaggaa gcaaatgttt aaaggggtat tgtttgattt agacggtgtc
    48301 attaccgata ctgctgaatt ccattatcgt gcttggcgaa agcttggtca ggaaatcggc
    48361 attacgatcg accacacatt caacgaacag ctaaaaggcg tcagccgaga agactcactc
    48421 tctttgcttt tagcttatgg cggaaaagaa cactcttttt caaaggaaga atttgcagaa
    48481 ttagcgaaaa gaaaaaacga ttattattta gagatgatcc agacgatcga accaaaagat
    48541 gtcttccccg gggtcgtgcc tttactggat tctttaaaag aagaaaaaat caagattgct
    48601 cttgcttctg caagtaaaaa cgggccattc ctacttgaaa aaatgggtct tacaccttac
    48661 tttgatgcta ttgccgaccc ggcagaagca acaaacggaa aacctgcgcc agatatcttc
    48721 ctccttgcag caaaagctgt cggactaaaa gcagaagaat gtatcgggat cgaagacgcg
    48781 caagccggta tccaagccat cctctcaagt ggcgctcaac caattggcgt gggacgtgct
    48841 gaagaccttg gagaggagat cccgcttgtc cctgatacga cttttcttac gatcgattac
    48901 ttgaaagaaa agtgtcatga ccatggataa tccagagatc tccgtcgtac cagttccaga
    48961 gactgaaata aagaaaatca cattgaagaa cgactactta gaagtcgttc ttctcaatta
    49021 cggtgcccgc ctgcaccaaa tcttcgcacc agacaaagaa ggaaaatcag aaaatgtttt
    49081 attgtcttac gatcgtttcg aagatgttct aacagacaaa agcttttttg gagcaactgt
    49141 cggacctgta gccggaagga tccgggatgc ttcttggggt gagcatagat tggagaaaaa
    49201 ctgcggatca caccatatcc acggtggcac gaaaggctgg tctttccaat tttgggaagt
    49261 agaagttttc aaaactccat catcaatagg tgtggtcttt tacttaaaag atgaattttc
    49321 tacctatcca ggaccaatca ctgctacgat cacttaccgt ttaacaaaaa acgaattaga
    49381 gatgacaacc tctgctagta gccttgttga aaccatctgc aatccgacca accatgccta
    49441 cttcaacctt tcaggaaatg gaacaagaga catctatgaa catcagttaa cggtcttttt
    49501 agatggtgtg cttgaattag atcaagaaaa actgcctact ggaaattgga caaaaaaaga
    49561 aaaactgcca atcgacttta gaaaatcccc aacactgcaa gagatccttg catgttatcc
    49621 agatggacta gatgatgtat ttcttttgca tcatcctagg ctttccaaaa cttccttaca
    49681 attattcgag aagcattctg gtcgccaaat gactatagca acttctaata aaagtatggt
    49741 attattttct acgactggat tcgaagctga tttctctgtc aatggaaaac agatgcattc
    49801 aaactacgga ttagcaatcg aaccgcaaga aatccctgat atcgttcatt tccccaaatt
    49861 tggatcgatc aacctccatc ctggtcaaga acggatcagt cagacgattt atcggttctc
    49921 tgcacaataa caagagaaag gtgtttttat catggctatt accgtaaaag atgtggcgaa
    49981 aaaagccggt gttgccacct cgaccgtctc tcgtgtcatc aacgatcatc ctagtatttc
    50041 cgagacaaca aagaagaaag tccgaaaaat tatggatgaa ctaggttatg ttcctaacat
    50101 gactgcccgc aatctaggaa aacgcatttc cagtgccatc ggcgtaatct taccccctct
    50161 tgattcaaaa gagcggatcg gtaacccatt ttatcttgag atcatggaag caatcaacga
    50221 agaagcgcgg aattatgata tgactacagc aatcgctact gctaaaaatt ttgaattgct
    50281 gttagagaac gtccagcgta tgcatttaca aaaacaagta gatggtttta ttctagtgta
    50341 ttcagatagt caagatcctg tcattgatta tttataccaa aaccacatcc ctttcacttt
    50401 gatcggacag ccttatcatc atgagaacga aatcgtttat gtagataatg ataaccagct
    50461 tctaggtaaa caagcaacag aatttttgat ccagaatgga cataagaata tcctgtttgt
    50521 gacaaacacg actcatgaaa gtttgtattt cgaacgatat ttcggttatc aaaaagcaat
    50581 gatgatggct ggtcttcctg cgttatccag tgtagtacta gagcatccgg aggattatct
    50641 gtcctttgag aaaacgttga aagaaaacga agccacagca ttggtggtca ttgacgatat
    50701 cttcgccctt cgcacgatgc agttggcgca aatgtacggc tatgtcattc cagagacact
    50761 ctccgtcatt agcttcaaca actcgatctt ctcaacatta acacaccctt accttacaag
    50821 tatcgatatc gatatctccg aattaggacg tgtcgctatg caaaagctgc gcgaattgat
    50881 ccatgaagaa ttgagcagtg gcgtacgttt ggtcatccct catcgcttga tcaaaagaga
    50941 gacagtcgtt cctttagaaa acccatacta aatagctgac tcactaaata cgctgaccat
    51001 ttatcctatg gtcagctttt tatgtaaaag aagatactac ttctaacaca ttgtttatct
    51061 gtaaaaaaga gcgtgggaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat
    51121 aaacggcgga agcagaagca actccttcgg aaataagctg aaattcacaa aaatttaaaa
    51181 agcaattttc ttgagtttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt
    51241 ttttccttcc ttcaattttt tacacgtcga cgattcaata aaaaaccgcc taatattgta
    51301 aagacaggtg caagaatcga gaaaaagacg aatggcaagt aatgcaacgc gctaactttc
    51361 atcgttccca taatgaatgt gccgctgact ccccacggta cgagagaatt gacggtcgca
    51421 cctgaatccg ctagtgttcg tgtcaaatag gtttttgata agccaacgtc atcaaaagaa
    51481 cgcttgaatg tttctcctgg aagaatgatg gacagatact gttctccaac gatcagattg
    51541 attccgatac agctcaaggc agtaaacccg actaaacgag aaggtcggtc catcttttct
    51601 tttaattctt gaacgatggt atcaacgatc agatatttga tcagtaaccc acctaatgcc
    51661 aatgcaagta aaatcaaact agcagaacct aacatactca tgatcccgcc tcgagaaagc
    51721 agttcatcta gctttggatc aggtgtattt gcgacataac cattcatcaa gatgtctgct
    51781 gcttttccga ccgtcaaact tttctcatgg ataaaagaaa gaacgagagc agctgtcgat
    51841 cccgctagta atgtagggat agccggaatt tttttgattg cccccccaaa taaaataact
    51901 agtggaatca atgtccaagg agagatccaa aagctcgctt tcatgttttg gatcatttca
    51961 ttgatactag ctagatcggc tgaatgactg ttatgtccta atatcgtata aaaaatcaac
    52021 gaaaggacga atgctgggaa atccgtataa cgcataccag cgatatgttc gaatagatta
    52081 actcctccaa tacccgctgc caagttggtc gtatctgata aaggcgagca gttattccct
    52141 aaaaaggcac cggaaacgac tgcgcctgta gtcattgcat cattgaatcc aagtaaatga
    52201 ccgatcccca aaaaagcaat tccaatcgtc gaaatcgttg taaatgaact gccgacagtc
    52261 actccgacaa ttgcacaaac aagaaatgct gtcggtaaaa agaaacgcac agaaacaagt
    52321 ttaaatccat aaaccataat cgtagggatg gttccagata aaatccatga agcaactaag
    52381 acaccaatca aaagaaaaat caggattgga atgatccctg gagtgattcc tttgatgatc
    52441 ccgtcatgga tctcatccca tgtgaacttt ttaaatctgc cataaaacaa caaacacatg
    52501 aaagctgcaa ggatcgggat atgtggcgag agctggaatc cgatgatcag tactcctagg
    52561 agtgccaaca acacgatcaa tacagcgata ctttctttta aagaaatatt tttattcatc
    52621 gtcgatctcc tatttcaaat gaaaaggccc tgccatgaaa aaactattca cttggcagga
    52681 cctcctacta aatcagcgac aaaacgtcgg ctgaaagact tatttttttt atttttcttc
    52741 taaacttttt tctacctcaa tcccccaaga atctttcttg aagtgattga tgatcaatgg
    52801 aataacgaac atgaccactg tgatagctgc tactaataat gcatcactca atgtcgctgt
    52861 acctaatgta gcaaatacga caacgatgat agagaagatc agcatgcagc tgactaccca
    52921 agctaatccg tttccgtttt taccaattcg gtaaggacgt tcgaattctg gatgtttctt
    52981 acgcatttta agcaatgaaa cagcaatcaa aatgtacacg atacaataaa taacagttgt
    53041 agcatttgta agtgtcaaga atacagcact tacgtcatcc attacaccat aaagcaaagc
    53101 aaataatgaa ataacgattg attgtgttag aacgacattt ctagatacgc cgtatttgtt
    53161 ttctttatgg aatccgaatt ttggtggaag gaatccttca cgagctactt ggatgattgt
    53221 ttttgatggt ccagtaaccc aagctgataa ttgtaaaagc acaccaataa ataccatgaa
    53281 gctgaagata tttccgatca cttctggtaa gcctaggatt tgacagtcaa tcaagatagg
    53341 ctgcgtgata tttgctaatt ccatttttcc atctggtacg acatttgcta caagcatcgc
    53401 attgatgaca ttcaataaaa ctaatccgat caaagcgata aatacgcctt ttgtataatt
    53461 ttttgttgcg tctttcaaac gaggcatgta aacagaactc atttcgatcc caacaaaaat
    53521 aaatgtgata ccagctaagt atttcaatga atcaatatgt tccaagtcag ggaaagcttt
    53581 tgaccatgaa aagtcaccta gatagccgtt tgagactagt ccgactttga aggctgccaa
    53641 aacacctaaa acgaacatga tgactactgg gatatatacc cctagccata caccgatgtt
    53701 cccgccgact tttgccatat cgaatttcaa gttcaagatc gtgatgatcc agtagataac
    53761 taaaatacaa cctaaaacga accagtgatt attgccaagt tctacattac caaatgtgtt
    53821 tcctaacaat ggtcctaaag tagaagcgac cattaccata cctgggaaca tttgcaccca
    53881 taatagccat gcaacgacaa atccccattt gctgcccagt gctgttttta cccataactg
    53941 aggaccgcct tcttgaggaa gcatcgtgga aagctcaccg gaaaccattg atatcggacc
    54001 ggcaaaaaac aaaactgcaa aaattgaata aaaaatcaat gtccaaccga ctgcggctaa
    54061 cgtaggaata ctccgaaccg ttgcacacag tgccattgtc agaccgataa aggtcccaag
    54121 acccatttta cttacttcag atttactcac cgttgacact ctccttgatt ttttggataa
    54181 aataacggtg tctttgaagt agtttcagca ataaacgaaa caattcagcg aatatttgag
    54241 gggattcttt gagtctattg cttgaagggt ttcttcaaag acaacctcaa aacaaaggga
    54301 tcgaagagct taggtctccc cttgctcttc agatccaaac atgcgtcaga aacagttagt
    54361 attcaagctg ttttctaaca tgattacctt gcttattttg cttcgcttgc caataattgg
    54421 tctgcgtaga ttttttccaa tttttcttga agagctgctt tgattttttc agcatattct
    54481 tcaaagtgtt cttctttgtt catgtatggt gtcattacgc tggcacgcaa tacagttact
    54541 tttccggcac ggttccattc ttcatctgag aatcctagtt ggttcacaaa ttgtaatggg
    54601 ctgttgccat agtctgggat agcaaagtca gtatgtgaag ttaagaactc attaccgtaa
    54661 atgcttcctt tcacgtatga agaataatca tatacgtcat ggttcaattt attcattgca
    54721 actaagtcat cgttgccttt ttctttgaat acatagtcta ccatattgaa atctggataa
    54781 gttaatggat gaacttcgat ttctttgtct cctactttaa atgacaagtc attcaagaag
    54841 ttgtagaaac gatgtgcacc ttcgattgat gcacccatta atttaccata tcctgtgaca
    54901 ttcaaaggta agacatggtg tgctgcccaa acgctggctg ctgttgcccc tgcttttgaa
    54961 ccttccaaga tataagcccc aagtaatgct gggatatctg cgcctttttc aaatacatat
    55021 gtcgcaaagt aagagataac gtctctcatg cggatatctt tgatgacgat accaccggca
    55081 gaatatggaa catagcccat tttatgtggg tcaatcgtta cagattctgc ttcttcgatt
    55141 gctttatatg cactgtgtac ttcttctaag atataatctt tgttttctgt aaagacattg
    55201 tatttgtaat gaacatcttt taaatcttca aatggaatga agttgttgtc ttcgtctaag
    55261 aaaatcgcac ggccgtatcc gccataagca gcatctacgt gtaagtagaa gtaaataccg
    55321 tctttttcta atacgcgacg caatgcaacg attttatcga taccgtcgat tgctccttct
    55381 tctgttgatc ctacaacacc gactacacct aggattggtg ttttttcagc tgctagacca
    55441 cgaacgattt tttcaagttc gttgatgtcc atgcgatagt tgtggtcaac tggcactgga
    55501 atcacttggt ctaaaccgat accgatgatg tcggcagctt ttagccatga atagtgttta
    55561 gtttgtggaa ccaaccattt tcctaatttt tctaaatgtt ttccgctacg tgctgagtga
    55621 gctttgattt cgtcgatttt ttctgggacg ctgtctaaca gattcatgat ttcttctgtt
    55681 gacaagttca ttaattccca atcgcttttt ccagcaacta gttcaggtgt cacttctttc
    55741 attgcaagcg gcaatgattt gatgttgcgg gcataccaaa gtccttctaa gtttgctaaa
    55801 gaaccatcag caacgatgtg gccccagcca tctttatagc tcattaattt tgcaaattcc
    55861 attcctactt cttcttccat ttggcttgtt gcaggagaag attcataggc aacgttatta
    55921 ccattccata gcatagcaaa attgtacgct agtaaagatg gcatcaatgt ttcagagttc
    55981 atatgacccc agtaacgtcc ggcattatgc caaggaacgg aatgggtacg catccgcgct
    56041 gaaatttctg atagaacatc ctttgtttta ttcacagtgt gttcaaaact agcactgctt
    56101 ttttcttctg gcgtaataat aggcatgtct tgaggcatgt agttttgacg ccagcctaaa
    56161 tgttcatcca ccaattcatt caacaaagct ttatagattt ggccgttctc agctttgtcc
    56221 ccaatgaata gggcatttag gtttagatct ttcgacaatg attcactcat agtgaaaccc
    56281 tcctaaaaaa atatttaaat tttgtcgagc cctacattac acctgttctt ctattctgta
    56341 aactaagttt tggcctgatt tttaatcata ttttaatatg tatcaattat ccgaattgtt
    56401 cacacttttg aaataattaa aaaaaacgag ggaatttaca aatgtaaact ccctcgcaaa
    56461 gattgtatta acgacaacca gcgttctatt ttaggatggg tttcaaaatt ggaatgttgt
    56521 aatagtattt ccctttttca aggtaaaaac ttactaaatt ctaatcaatt actttggcca
    56581 aaaagtaatt tttcttacct tttcgaataa ccacaaactt accatcgaat tcatcggatg
    56641 gatttatgac aaaatttaaa tcggtaacgc ggtcaccgtt aatacttata gctccgtttg
    56701 aaacatcttc acgtgcttga cgtttagatg gttcaatttt tgtggaaaca agcagttcca
    56761 cgatattttc aggtgtgctt gaaatttcaa cgttcggcat tttaccgaat ccttgcgcga
    56821 tttcttccgc atttagttct ttaatatttc cagagaacaa tgcttgcgtt attttttgcg
    56881 cttctttcaa gtcttcttca ctatgcacaa atcttgtaac ttcttctgcc aaacggcgtt
    56941 gtgcttcacg tttctcaggt tctgtctcta cttttttcgc taagtcttcg atttcttctt
    57001 gagacaagaa agtaaagaat ttcaagtatt tcactacatc gcgatcatct tgattcaacc
    57061 agaattggta gaactcaaat ggtgaagttt tcttcggatc aagccaaacg gcaccgcctg
    57121 cagtttttcc gaatttcgtt ccgtcagcct tcagcatcaa tgggatcgtt aaaccgaacg
    57181 cttctacttc tggtccttct aattttcgga ttaggtctaa tccagctgtg atatttcccc
    57241 attggtctgc tccaccgatt tgcaattgca catcgtatgt tttatgcaga tgcaaaaagt
    57301 cgatagattg cagaatttgg taagtaaact ctgtaaatga gattcctact tccaaacggc
    57361 tggcaacgat atcttttgct aacatcgtgt tgatgttgaa gtttttaccg tagtctctca
    57421 aaaattcaag taaagagatt tttgacaacc agtcgtagtt gttcacaaat gtgatatttg
    57481 catctttacc aaacaatttt ctcatttgat tggaaagtgc gtccacgtta tgttgcactt
    57541 gttccatcgt ttgcaacaca cgttctgttt tgcggccgct tgggtcgccg atcgttcctg
    57601 ttccgccgcc aatcagaata tatggatgat ggcctgccaa ttggaatctt ttcatcatca
    57661 taaatggaat caaatgtccg atatgcatcg aatcaccagt tggatcaacg ccgcaataaa
    57721 gtgaaatgct cttttcttca acgagctcgc gaagtctttc ttcgtttgtt tgctgattaa
    57781 tggcgtcgcg ccaagttaat tcatcaataa tattcatttt actgacctcc gtgtagtttt
    57841 tttatgaaag caggaacctg ctagcatgtg taattaatta tttcttcagg ataggatagg
    57901 aaataaaaaa agtcccttgg catataagcc aagggacgaa atctcgcggt accacccaat
    57961 ttgtatgtct agatatccaa catacctctc gaacatttaa cgctgtcgcg tggcaatcac
    58021 tgccaaagac tcctgaaatg taattcgctt taaagcatgt actggtttgc agcgaccacc
    58081 agctttctga aacattggct ttaacaacta cttcgtttcg atcttcgtct tgttattatt
    58141 aatatgtgtc tatcatagag cgcaactttt tctttgtcaa cagaaaaagg atattttttt
    58201 taaattcctc attttttctt ttttgaatcc gctctaattc cgaacatttt attttcaatt
    58261 attgttaaaa gaaataactt tttctgtatt ttttctgcaa tttatcgaga agtcacttta
    58321 ctttgcctct tattctttct tcgttacaat aaggaatggg acaagtaaaa aaatgaaaaa
    58381 gcaagggacg aggtatatgg aaaaaaagca aaaaattatt ttaggagcca cttctgcttt
    58441 aactatcgtt attttaattc taagtttgtt ttttgtcatc cgatcatcta caaaaaaaga
    58501 accaatcatt cctgaaaaaa aaataacaga aaaagcaaaa gaaccgacag atgataagca
    58561 acaactagtt actcaagcaa aaaatctggc tcaaagctac gattatgacc aagcgattgc
    58621 gttattggaa aaagatagta agaacggatc tactgaaaca caacagcttc ttcaaacact
    58681 aaaaaaggag aaagaacaac tgatcgactg gtctgatcct actcagatct ctcatgtctt
    58741 tttccacagc ttgatcgtgg acccgcaaaa agccttccac tctcaacagg cacaaggata
    58801 caaagattat atggtgactg ttgaagaatt caaccgttcg atcgaacaac tttatcaaaa
    58861 tgattttgtt ttagttaatt taaatgattt gatccaaaaa gacgaacaag gaaatctttc
    58921 ctttaacggg cttgcgctac ctgaaggaaa aaaaccattg gttctttcac aagatgatgt
    58981 cagctattat gaatatatgg ataattccgg ttttcctagc cgattggtcg tatcaaataa
    59041 caatgaaatc aaaaatgtct ataaagagaa agataaagaa gaaatcggtg actttgacat
    59101 ggttcctctg atcgatgcct ttgtcaagaa acatcctgat ttttcttatc gaggcgcaaa
    59161 aggtaccttg gccctgacag gttacaacgg agttttaggt tatcgaacat caaaatctca
    59221 atatggagac aatgaaaaaa caaaaaaaga gattaaagaa gctaaaaaag tggcggaaca
    59281 gttgaagaaa gacggctgga gctttgcttc ccatacatgg ggtcacttga atatgactca
    59341 agcttcacta gaagatgtaa aaaaagacaa tgaattgtgg cagcaagaag ttgcacctat
    59401 cctaggaaaa acaaatattc tgatttatcc atttggtgca gacatcagcg actggcagcc
    59461 ttattcagca gaaaatcaaa aatttgctta cttgaagagt caaggtttcg atattttctg
    59521 taatgtcgat gcttctacac cagcttgggg acaattaggt acggatttct atcgaaatgc
    59581 tcggatcaat atcgatggca ttcgttttca atctgattta gacggaaaaa atcctattct
    59641 tgatccattc atcaatgtcc atgatgtcta tgacaaagaa gcacgcgaat ctgcttgatt
    59701 ttacgtcatt tatgaaccaa aaaagccgaa ctgacatttt atcgtcagtt tcggcttttt
    59761 tatgctttat ttcttcttcg gtgcgattcc cttgatcagg acttctgtcc attctgctgt
    59821 atatggaagc agttttgcgt atatctccgg cttagaaatt ccttctacca ttgcttgaaa
    59881 atagaggatc aagaactcat ccgaatattt caaatccact tttccttctt tccgccctcg
    59941 atggaaaaga tcaagcatga tactgaaact ttcttgcgta tacgcatcca tcgcgtcaga
    60001 aaattctttt ttttcttttt ttgtatagta tgtcatcaaa tcgatataaa attgtttgtt
    60061 cactcgattg agataacgga ttttgttttc actcatggca atcagtgttt cttcaaatgg
    60121 aagattttcc gcaatcaacg ctttcgcttc ttgccctaaa tcattcataa atgcaaaaaa
    60181 tacttcataa atcagccctt ccttgctgcc aaaatattta aaaatcgtcg ctttacttac
    60241 ctgagcgttc tcagctacct tatccattgt cactccagcg gttcctatgt ctgtgttcat
    60301 caagtcaaaa gctgcttgta aaatctcttt ctttttctct tgtgctctct tttcaaaacc
    60361 gttcatttgt agagcctccc gtaattttaa acttttataa tatatatagt tcaaatttat
    60421 tctgaggttt attataacta tctattgaga aagtatcaag ctagcaattc cttgtttatg
    60481 aattcattga cagaaattta taaaaagaat attatgtact aaaataataa aaaaagtttt
    60541 ataaaaaggt ataaaacctt tttattaaac tatttaatct tgtttagtat atatttttag
    60601 aaaaaggagt ggggttttta tggcagaaat cgtcaaagtc acacatttga aaaagagctt
    60661 tggtcgtttc gaagctttaa aagatgtctc tttcaccgta cattcaggtg aagtcgttgg
    60721 atttatcgga ccaaatggtt ctggaaaatc cacgacgatt cggacacttt tagggattat
    60781 aaaaaaagac ggcggaacgg caacgatttt tgggcaagat gtatgggaag atagtttatc
    60841 tatccatgaa cgtctttctt atgttccagg cgatatctct ttgtggggaa atttgactgg
    60901 aggagcgatc atcgatctct tcatgaagct tcatggaaaa gggagtgctc aaaaaaaaga
    60961 tgaactgatt caacgtttct ctcttgatcc taagaaaaaa gcaaaaaact attcaaaagg
    61021 aaatcgccaa aaagtgggct tgattgctgc tttggctgtt gattcggatc tctatttatt
    61081 tgatgagcct acttctggtt tagatccttt gatggaaacg attttccaag aagaagttga
    61141 aaaattgaaa gaacagggca aatccatcct actttcttct catattctca gtgaagtcga
    61201 gcgactagcg gataaagtcg tgatcatacg cgaaggaaca gtcgtagaag aaggaacact
    61261 cgatgaattg cgtcatttga ctcgttcgac catcactata gaaacagaag cagagaatag
    61321 tcacttagca caattgaacg gtgtccatga ctttgtgcaa aaaggtcacc tcgctcaatt
    61381 tgctgctgat aacgaagctt tcgatgtgat tttagctgaa gcgtccaaat taggtgtgaa
    61441 aaaatttgaa tctattccac cgactcttga ggatttattc atgagacatt atgaaggatg
    61501 aggaggaagc gaaatgactg ataaattttc acgctggtcc accctttttt ctcaaaacct
    61561 tagccgtgac tggaaaaaga tcctgatatg gctagtcggt gtcactgctt tttctggtgg
    61621 ttttgtccct gcgtttgaag aattgacttc cggacaagga ttagctggat tatacgaaac
    61681 cttgcaaaac ccggcaatga ttgcaatggt cggaccaact ccagcttcga atgctgctga
    61741 gtatacttta ggagcgatgt atgcaaatga aatgttgctt ttttgcagtt tgtttgctgc
    61801 tgtgatctct gttttacacg tggtgagcca aacgagaaaa aaagaagaac ttggtctgat
    61861 tgaacttatt cgttcctttc gagtcggacg tcaagcaaac tctttagctg tcatcgctga
    61921 aagtattctg atcaatttgc ttttagcaat atcgatcagc tgtacgatga tttttttcca
    61981 agcagattcg atcactgcag aaggtgcttg gctttttggg ctatctgtag gtagtatggg
    62041 gattttgggg agtgccgttg cactgtttgc tgcacaactg atgccaagtt cagccagttc
    62101 gaatggtctg tctttagcgt ttttaggtgg actttacctt ctacgagcaa taacagatgt
    62161 agctgattcc tccctttcga tgtggaatcc tttaggctgg tcttatttga cctatccatt
    62221 tacaactaac aactggattc ctttgcttta tttgtttttg ttcagtctaa tcttgataat
    62281 cagttcattt gttttagagg aacatcgaga tttgaacacc ggctatatcc ccgaacgtga
    62341 aggaaaaccg tatgctaaac gttctctttt atccttgcgt ggtttattat tctatctaaa
    62401 taaagggatg acaatcagtt ggcttatcac ttttggtttg atgggggcgg catacggctc
    62461 tatctacggg aatatgcaac agtttttaga aagtaacgac ttaatgaaac agatgtttat
    62521 ccaatctggc gtctcaattg aaaaaaactt tacaagcacg atcatggtcg tcttgatttg
    62581 tctagctgct attttaccaa ttgctagcat caatcgttta tttacagaag aaacccacgg
    62641 acgtatgaat cagctgttcg caacaaaaat ttctaggaac caactttatt ggaccaatat
    62701 gttactagct attctatttg ctatattggc aatcctgatt agttctggtg cactagggct
    62761 ttctgctttg gcttcgatga aggatgcgaa agacatggat ctattgatgt ttcttaaagc
    62821 cgggttcaat ttcctgcctg ccgttttagt gtttatcgga attagtggct ttctattcgg
    62881 atggcttcct cgaatgggta aacttgctta tatctactta gcttactcct ttgcactcaa
    62941 ttactttggc ggaattttgg atttaccaga atggttttcc aaaacagctg tccctagctg
    63001 ggttccacgg atgccaaacg attcttttga tctttccgtg ttccttacgt tatcatgtat
    63061 cagccttgca ctgttcgtcc ttggttctat tggttatagg cgaagagact tactagaagg
    63121 ataataaaaa agcatgtaca atttcatgct agcctccaaa tctgggtcaa aaatccaaca
    63181 tttggaggtc atttttttat ttacaagtct tctaactgct ctttatcaat gatccagact
    63241 tctcgattct ttctttttac ataaccattc tcctctaata atttaaattt tctagataaa
    63301 gtttcggggg tcgtaccgat aaatgcagca agttctttca ttttcattgg caggaaaaag
    63361 tgatccttcc ctgtcgcttt agctaagtca agcagataga cagcgactcg ttcttctact
    63421 ttttccatca tcaaaaaagt tgtctgctgc tgtacttttg ttaatttctc aacgttgatt
    63481 gctaaaagtt tcagactaag ggcgggataa tgcagtaaga gttccgagaa atctttttgt
    63541 ctcaatatgc aaatttccgt tttttctagt gcttcgccaa ataaagactc atttggaacg
    63601 cctagcaact ggctctctcc ctcatatccg cccggctcta ttactcgtaa aagctgttcc
    63661 tttccgctag cagataattg atacactttc atactgccgg aggccacgat cactaattga
    63721 ggttctccat tcggcgcaag aatctgttct cctttttcca catagtgatg tttgacaagt
    63781 gcgtgtactt tttcttgatc ttgctgtact aagtgattga acaaaggaac aaatgaaaca
    63841 cataaatgat catgactcat aatctctcct ccttctaaac agctatttct aataaagaaa
    63901 cataacttcc cttattccaa ctgataattt tcttttatca tagcataaac gtcaagcgaa
    63961 ccagatacca gcaaatagag tggatttctt tttgtccctt gctttatttt ccttcttttg
    64021 agaccttaag taattgattc ctatcaattt tttaaatatt tactttttga ataatcaaaa
    64081 acaaaccttc actcgcaagc cgagagtaag gtttgttttt cgcattgctt atttcgtgtt
    64141 gtctattgaa atttttccgc acctagcttt tgatatagtg gtgctagttc ttgtaaggtt
    64201 tcagcggtta tttgctcaac gtgttcgatt tcttgcggat agaagaatag tttacccatt
    64261 tgaaaggttc ggtctctcat cgcttttttc aactcttctt gtttctttgt atcggacaaa
    64321 tagtcagaca aagctaagtg gtcgtcaagt tcatgttctg gttgcggcca tatgtaataa
    64381 tccttgatat cgactgtttt gctccactct ttcagtatcg caagcaactg gttatacgct
    64441 gcaatgctac catatcgttc ttcgctttta taatgttggt acatcaaata gatttggtat
    64501 tgtttttgat tcaacaaaac tgctagacag gcattctcgt cttgacgaga ttcgcttcgg
    64561 tacgcgctcc aaaaatgact gcgtaaattc cagccattcg tccagctttc gattttaggt
    64621 ttgctcatgc cgtacatatc aggcaaaagt gcagctgtct ggagctgaat ctctttccat
    64681 ttttgccagt gttctttata ttctttcttg atttgttcaa tcttatctgt tggatggctc
    64741 tctttgagct gtttgaatgc gtagattggt cgatcaaaca actgataatc tgattttcca
    64801 agttccattg tttcgcctct ttccgttatc ttttgtattc tacctgaatt ttgcgatatg
    64861 gacaaataga ggttgtgaaa aagctgttct gcatcaagtc ataagaacag ccaaacaaaa
    64921 aactgcgatg agcatcaccg cagttttatt gaacttaagg ttgattgatc acatctaaaa
    64981 gcgttgaaaa taaaatggca ctagaagcag ctcctgggtc gatatgtccg attgcacgct
    65041 ctcctagata ggaagctcgt ccttttttag ctaccaagac tttggtgtgt tcttttgcct
    65101 ggtcaatgat ttcttttgtt aaattccctg ccttcaatgc ctctgtgact ggcagccaaa
    65161 catctaacat cgttttatcg tcagcttctg cctttcctcg gctttgaata cccgctgttc
    65221 cctgctcgat cacttcgcct aatttttctt gagaatcaat agaatcgacc tcttttgtcg
    65281 ctttcatcat gttcatgaat gcagatccgt ataacggacc agaagcccca ccgactttcg
    65341 atatcatcgc catagagaat actttgaatg tatcgctgat tgtttcagga tgatcggctt
    65401 gaaaagcttc ttcataagct gaaacccctc ttcccatgtt atttccatgg tctccatcgc
    65461 caatcggggt atctagatca ctgagatatt gtttgttctg attgattgcc tctgcaaatt
    65521 gcgtgagcca ttcttggatt tcttgaacgg ttagtttcat attatcaccc ttttatcttg
    65581 agatttttta ccatgaaatc gtctgtacat tgctttctaa agaatctttc cattgtgtct
    65641 ccgtcaagtc gatcagtgtt agagaaagac cttgcatatc tagtgaggtc ataaaattac
    65701 cgacttttcg gaaaactaca ttgacgcctc tttcttccaa cagagctaaa acatcattca
    65761 tgaacacgaa ctgttccatc aaaggcgtcc cgcccattcc gttgactagt acacctgctt
    65821 cttttggtaa attcgagtaa tcatctaaaa tcttagtaac aagttccttt gccagtactt
    65881 tagaaggctg catcttttct cgacgatagc ctggctcacc gtggatacca acaccatact
    65941 cgatctcatc ttctcctagt tcgaatcctg gtttccctac ttctggtaca gttgctgctc
    66001 gaagcgctag accgatcgtc ttagttgcct gtacgacctt ttctcccagt tctttcaact
    66061 cagctaggga agcaccgttt cttgctgcat cgcccacgat tttgtgtaca aggacagtac
    66121 ctgcaactcc gcgttttcca gcagtatacg tactattttc aacggcgata tcatcatcta
    66181 cgatcacact ttctacttca atatcgtcca tagcagctaa ttcctttgcc atatcgaagt
    66241 tcaatgcatc ccctgtataa ttcttaacga tcagcaaaac tccttttcca ctatccgctt
    66301 ccttaatagc cgtctgaatc tgatctggag ttggagaagt aaacacatca ccaagcaccg
    66361 ctgcacttaa catcccttcc cctacaaacc cggcatggga tggttcatgt ccgcttcctc
    66421 caccagaaac caaaccaact tgttccgctt tctgattctt ggctacaaca cgggaagatt
    66481 ctacacgatg aacaagttct ggatagcttt ttactaatcc ttccagcatt tcttcgacaa
    66541 tattgcttgg atcattcatg atttttttca atttattccc atcctttctt tccggcattt
    66601 tgtgcttctg ttaagatttg ctgagtatca tctgttatac cggcagtgat ggctgctgca
    66661 aaagctcctt ccactaaagg cgcatccacc agatgataac gttgctgctg ctcttcttcc
    66721 aacatgtctt ttgccagttc cgagttcaat accgcgctac cgatatccgc aaaaacaaaa
    66781 taactagctg catcgcttga ttcattgact gcttctacga ttttcatcgg gtctgatccg
    66841 atctcgccat ttattccgcc gagagaaaaa atttgcacct cttcggattt ttgcatctgc
    66901 tcaatcattt ctttaatacc atctgttact ttttgactat gagaaataag caaaatactt
    66961 tttttcacaa gaatcttcct ttcttttaca tagttgctaa cgattggttt cgctttcatt
    67021 aatgaacaat tcaagtatat aataaatttc taattttggg aagatatgtg tagtgcttct
    67081 tgaagtttgt tatactttaa ttaaaagaga ggagtgatcg aatgattaca acaacaacga
    67141 acagtgtaga gggaaaagcc gtagtagctt ataaagggat cgtttttggt gaggtcatta
    67201 ctgggatcca tgcatttaaa gacttggagg cggggttgcg aaatatcttt ggcggccgct
    67261 caaaaagcta tgaaaacgaa ctaatgggtg caagagaaga agcacttgcc gaaatggccg
    67321 aacgtgcaaa agcattagga gctgatgcga tcattggcgt aaaaatggac tatgaagtat
    67381 taggcagcga caatggcatg ctgatggtca catgtagtgg aacagctgtc tccacgagat
    67441 aagtagggga catctgtttt aataactaaa gagagatggg acatgtagtg gcactcaaga
    67501 attagatttt tcagtctaac ttcgaggtac tctaccttcc catcctctct tttttatttc
    67561 tttttgcgga tgattccgta agctgctgcg actaacacac ccgcccctgc taaggaataa
    67621 ctgacagaag ctgttttttc atttgttttt ggcaaagtct gacttgttga tttggatggc
    67681 tctgtcgttt gctgaactgt ttctgtcgtg cttccagagg tcactgattt ttctcctgcc
    67741 gaatgcaaaa tatcttttat tcgttggaaa ctagctgcta agtcagcttt ttttgcttca
    67801 aaatcattag caactgtttg tgcttcgctg atctttccgt ggattgcttg actggccgct
    67861 gtgatatccg cttgagtttg acttagcttt ccagaagctg tttgagcaac ctgatttaat
    67921 tgctgattga attcttttgc ttgttggatc ttcccgtcaa gatctgtttg gacagcttcc
    67981 gcttttgctt tcgcttcggc aagctttttc tcaagttgtg cacggtcatt tcccacttta
    68041 tttttaagat cagctaaatc ttgatcgatt gctgcttggt cttctttcaa tttgtttttt
    68101 gcagcttcta atgcttcttc gagttctttt cttttatttt ccaagtcttg caaatgatcg
    68161 atttggtcag aagaggtaga actatttatt gatgtatctt ctgaagatga aatcgtttct
    68221 cctgaattat ctgtcggtac attggaagtc atttctgttt ccgaggcacc gttttcagca
    68281 ccagaagagc ttatagttgt tgaaggttct gtttgagcag gttcagaagt ttccgcaaaa
    68341 gctgtctgtc ccaataagaa aatactggaa acagcaattg gccagattaa cggcttcatt
    68401 ctttttttca tcacttttcc catccttact actttttaac taatttctta ttataagcaa
    68461 aaatgtttgg aagaaccata aacacaacag gctgtttgtc taaaaaacgg tgcatctttt
    68521 atttttttgt acgttccggg cttttccatg tcccgcttcc ataacctaaa atcgttttga
    68581 ctgctggaat gaaagtcgtc acgatcgtta tcgcgttcac tatccagaac aaaagcatat
    68641 atagtggtgc gaaaagaagg tatttcaatt ttcttcgtcg atcatctaca atcagcgaag
    68701 ctagcaactg gaaaaatcct gcgaccattt caaagcagat gaacacaaaa ctgaatgtaa
    68761 aagtaatata aatctgttcg tagtttcctt gatatacata atgcccgatt aatccagcaa
    68821 aaagaactac ggaaatacaa aagaatattg accagacaat actcaatgtt tgatcaatga
    68881 agatgatcgt tcttccaata tgtttaaaag gatggagcat cacttttttg aaattcgtca
    68941 gccagacttc tgttccacct tttgcccaac gttttctttg tcgatatagc atcttcaacg
    69001 tcacaggtac ttccatgaaa aagataatct caggagcaaa aacagataac cagtcattta
    69061 actgatgatc ccaagcaata ctgatgtctt ctgtcgctcg atcttgtctg aatccgccca
    69121 catcgatcaa ggcttctttt cgatacatcg tatttgctcc actgtacgca taaagaccac
    69181 cgaaaacagc tgactgtgtt cgtttaatga tgcccacgat acttgagaat tcaaccgttt
    69241 gagattttgc aatcaatttg gtacgatttt ggacatccat atttgcagtg accgcagcaa
    69301 tatgtcttgc accagggcga atgaaataat tcacatacct aatcagtgca tctggctccg
    69361 gtaccgtgtc tgcatcattg cttaaaatca atttcccttt tgcaaaggca agtccgatat
    69421 taaacgcgtg ggcttttcct ttattttttt cgatccgaac cacacgaaga ttagcgtatt
    69481 ttttcatcag tctagctaat atttctggtg tttggtccgt tgaaccatcg tctgtcacta
    69541 atacctcata gttgtgataa ttcaatttcg tcatcaaata ctcgattgta tcttcgatca
    69601 caatttcttc attatgggct ggcaccatga tcgtgatgaa cggttcgaca ctcaagggaa
    69661 catctaccca ttctttttgc ttatgtttgt ataaaaacac ataacaccag acaccaataa
    69721 accatacaaa tccgcctaaa atcggataag aaacgagaaa aaatacataa tagacaaaca
    69781 agtatctaag aataaattac ctgtatgggt gattgagatc atttcagttc caccccattt
    69841 tctttataca aatctgcaat gaatgtcgta tctagatttt tctcttcgga gacagaataa
    69901 aaacggaccg tttctctctc ttcttttgtt ccaaaacgtt ccgtatacac ttcttcaagt
    69961 aattcttgtc tttttttcaa tttttcatct tcatattcaa aagatttatt tacttttttg
    70021 tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa actgaaaaaa
    70081 atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa aaaagcgtac
    70141 aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca tataaaagga
    70201 agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt taaccagtaa
    70261 ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc tcttgttact
    70321 tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc atacatttca
    70381 tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa acatttctca
    70441 tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt tttatctctt
    70501 aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat cgccgtgtac
    70561 atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa gattacatga
    70621 tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc taatccatat
    70681 gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg ggtgataact
    70741 gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa caattcttca
    70801 gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga tacgatgtcg
    70861 atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc gctagaacgg
    70921 taactatttc taagatcata ccgatggatc ggcagatggt agtcgtcgaa acaacgcata
    70981 atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc tactaaagta
    71041 aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt ataatcttgt
    71101 acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac aagtaaatga
    71161 cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca agcataagct
    71221 gagccatatc gtttttcata aattctttca aacaactgat gctcatccac ccatgccatt
    71281 tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct tgcataagct
    71341 acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa ctgcctatct
    71401 tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt caaaagatga
    71461 ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat caatttagga
    71521 tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac tgtttttcca
    71581 tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc tgattgtttt
    71641 agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac attttctccc
    71701 gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag ctgtgaaacc
    71761 agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag gacattcgaa
    71821 atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga tgtcatcgta
    71881 tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt tttaccactt
    71941 cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat tgtgttttgt
    72001 tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg gcctaagact
    72061 tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac caaacgatct
    72121 ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt ttgacgaacc
    72181 gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg tgaacgccaa
    72241 tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat ataaaaggat
    72301 tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg acttttcaat
    72361 aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt catctgatcg
    72421 atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa gtaactgtca
    72481 aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc tatttttgcc
    72541 gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata cacgttcttc
    72601 agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct ctttttttaa
    72661 agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt ttaacgagta
    72721 tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag aaaatgcacc
    72781 atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta gatagctacg
    72841 taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat ttaatcggtc
    72901 ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt ccaattcttc
    72961 tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca attcatgcca
    73021 ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta gcatgtttac
    73081 tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg gtaaatcgaa
    73141 gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat ccacaccttt
    73201 tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga tcaacttcag
    73261 taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata catgaatgac
    73321 ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg atgcggcagt
    73381 cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc gagaaattcc
    73441 ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa gagaaataag
    73501 tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg cataaatatc
    73561 aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc ctaattcgat
    73621 tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa taactaaatc
    73681 agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt caaatccagc
    73741 gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt ctataaaagc
    73801 tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca ccatgacttc
    73861 taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg caccaacgat
    73921 cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat ctggtacatt
    73981 tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa caactggttt
    74041 tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac cctcatgtac
    74101 gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg gacgcacatc
    74161 taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt cccgttcact
    74221 gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat acggtccttt
    74281 ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag cagcagacat
    74341 tccacctgct acacctccga caataacgat tttcatctta tttccctcct actgttcgat
    74401 caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat ttctttaatt
    74461 ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg gtcttttctt
    74521 tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct tttatatgcc
    74581 cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc aaaagctttt
    74641 cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc attttctgca
    74701 cctcatttac atttttataa atacctcatg gggtatatta aacaggagct ttattaatat
    74761 gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata aaataccgat
    74821 aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt gataataaaa
    74881 atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa aaaatgatcg
    74941 aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt tctagtattg
    75001 atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag aaacctgata
    75061 gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt attaaaaagt
    75121 aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa atggtcagaa
    75181 acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat tgtcgtatct
    75241 gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt cttagatttg
    75301 caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat ttcagaaagt
    75361 tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt tgaactgcaa
    75421 tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga agtgatcagc
    75481 ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt gaatcaaagg
    75541 ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat agcttctcat
    75601 atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc gtttaaggaa
    75661 gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga aaatagctcc
    75721 tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca ccgtttaagg
    75781 aagagattgt gaaaaagtcg cttagtttcg agtattaaga aaaattttgt gaaaatggat
    75841 ttctacattt ttgcataaat ttggcttaat accgagaaac tggcttttga ataccgttta
    75901 aggaagaggt tgtaaaatca gcggtttgac ctgagtatta gagaaacaaa cggaaaaata
    75961 gctgctcata ttttgccgtt ttttgagcta ataccgaagg tcagctgatt gaacaccgtt
    76021 taccaagagg ttgtgagcct accatttaac tccgaatcac aagaaataat ttcaaaaaca
    76081 acttctcctg tttttggttt ggttggctta ttatcgagaa gtagctactg ggagtctatt
    76141 tatcaaaaaa aggaatgcga cataggtcac accccttttt tattttcttt gaaacatctt
    76201 tcggtactcg acagcataca agacaaaaga ttgtaaaaga ccgtaagtga tgacggcaaa
    76261 aatgactctc acccacagcg ttggtaattc tgggaaaagc agcaaaagga tcatgccaac
    76321 atagatgaca gcagtcgcta ctttgccata ccacttcgct ccacttaatt tgatattttt
    76381 agatagcccg attaatccag ctacaaacat aaagccttct tttatcaaga atagaccaaa
    76441 caacagccaa agccacggtc gataccatgc catagacaaa atcagaaaaa gttgtgtcaa
    76501 tttatctgca aacggatcaa gcaccttccc taagtctgtt acctgattat atctccgagc
    76561 aatgaccccg tctagataat cagacaaacc tgagacaaca atgaccgctc ctgctatata
    76621 aaatgactgt ctcagcacaa aataaagata aatcggcagc aaaaaaatac ggaatatcga
    76681 taaaagattc ggaatcgttt gtatttcttt acgccagtct gtgcttaatt tcattttatc
    76741 gccctacctc ctcagcactc ttttcttttt attttagctt acacggggaa taaaaccaat
    76801 gatttcactg tttttatttt tctttacagc cactgatatt atgagctaat ttgagataat
    76861 cacttgctgt tttcatcgtt tactcttctt tcttcctgtc gtttgcgcat ttcttttact
    76921 agtactttat gagctttttt tgatcctcgc tctaggtcac gctgccgttt acgtgtttta
    76981 taatcgataa acagactgtc taaatcgtaa cctgtcgggt acagatcttc tgctgaacgc
    77041 accagacgaa cacgctgacg aggcacgtgg attatttttt tgtctaaata tacatcaatc
    77101 gtatcttcac ctatatcttc ataaactaac gcttcttttt ggtattcatt gacaaatatc
    77161 cgatcacctt ttgcaaagag ctggcgttct tttttattct ccttcctatt tgtaactgct
    77221 gtcttaggaa gaaattcttt tttctgtgtc cgataggctt tttccgccaa gtaacgctct
    77281 gcttgatgga tcaatttcat cgacatatcc attttatgag caatccacaa agcttggctt
    77341 tctcctgttt ctccaacccg caactgatat ttaggacgaa gtgcttcccg atcgaaagcc
    77401 atagcagccg gcacaaaatc ttcatgatct ccggcgaatt tcttgatttc tccataatgg
    77461 gtcgtggcaa tgatcaatgc cccttgctca tacatagatt ccatgattgc gatcgctagt
    77521 gcagcccctt catttggctc tgttccactg ccaatctcat ctaaaagtac caacgtattc
    77581 ctttttatct ttttcaaaat agcagcgatg ttttgcatat gaccagaaaa tgtactcaac
    77641 gcattttcta aattttggtg gtcaccgaca tcgacgaaaa tctcatcaaa gacagcaagc
    77701 tctgtccctt cttttgccgg aacttgcata ccgaacatcg ccatcaaagt caataaaccg
    77761 actgtcttca acacgactgt tttgcctccg gcatttgctc cggtgataat caagcctcga
    77821 tagtctttac cgatttcaac atccaatggt accgcatgct caggtaaaaa tggatgtctg
    77881 ccttgcttga taatgatatg ttccgattta ttgattctag gtgtgcttcc attgatttct
    77941 cggctaaatt ttgcccgagc aaagatgata tctaacaccg tgatcgtttc gatcaataaa
    78001 tcgatcactt cttcgttttc tgcgatagca ccagtcaatg ccgccaggac ttgatattct
    78061 tcactgattt cttcagcttt taacaactga tagtgttcat tcaatttaga aatcacagtt
    78121 ggttcaataa aaaccgtcgt tcttttattt gattcatcga taatcgttcc atctatttta
    78181 tttttatagc tggctttgat aggaatcgta tagtattcgc ctttttgaac aatcattgct
    78241 tcttggatca tttctttgtt tttcggatga cgcaagaatt tcagtaattt tgactggatt
    78301 tctttttcga ttagttgtaa ttgtttgcgt actttacgca aattacgtga cgcgtcgtca
    78361 ctgactttat tattttttat tttctggtca atcaactctt ctacatttat caaatcagga
    78421 agatgtttgc tataagcaaa caacaagggt gtctggtatt gattcttatc aaaaaacttt
    78481 gtgatcatcc gactgcttct aagaaaatcc gcatattcga tcaaatcggt tggctgcaga
    78541 atcaaccctt ttttgacttg ttctgtcagt gcatcgatcc tcggcaaccc cataaatgga
    78601 acatgctgac tgctttctaa gatcaagcgt gcttctcttg tttcttcttg acgatccaag
    78661 acagtttgta ggtttgtcga gaccgtcata tcgctgattc gttttttgct atagtttcca
    78721 attgctcttg cttggatttc ctttttgatc cgatcaaatt gtaattgact aatcatttcg
    78781 ttgtccatat tctctccttt tcttgaagag atacctgtcc caaaataaat aagggcctag
    78841 agataaataa aaggatagaa cacagcttaa aaaagtcatg tcctatcctt attagaatac
    78901 aaagatattg ttttcctgac aggtaccgtt tcataagcac accaaataaa ctttgtttat
    78961 tggctattcg acttactgat tagataccag ctacactcac aaaacatacc ccattcttct
    79021 aggtcttatt tattttccac ttaactatag caagacacat tttggttgtc aaactttagt
    79081 atgttttata gatttacacc attaatgaat agacacctac cgcgataatt gctccgatca
    79141 ttggacctgc gattggtacc catgaatatg cccaatctga agttccttta ttagctattg
    79201 gcaataccgc atgtgctaat cgaggaccaa agtcccgagc agggttgatt gcatatcctg
    79261 ttggccctcc taatgaaaga ccgatggcta agatcaatcc acctacagcg aatacgttcg
    79321 ttccaggtgc aaattcgttt tgtccaaaag ctagcaaccc aagaactaat acaaatgtac
    79381 caattaattc tgtgatcaca tttgctggat aattccgtat agcaggacct gttgcaaacg
    79441 ttcctaaaat cgcgctttca tctttcgttg cattccaatg tggcaaataa gaaagccaaa
    79501 caagaatggc ccctgcaaaa gctcctaaaa cttgcgcaat gatgaaagga acgacaagat
    79561 tccaactaat tgcccctgtc atcgccatag ctagagagac tgcaggattc aaatgagctg
    79621 ggcccatgaa tccagaaata tagacagcca ttgttacagc gagtccccaa ccaaatgcga
    79681 tgacaatcca gccagaagcc tgtgctttac ttttgtttaa attcacagcc gcacacacac
    79741 catctccaag caaaactagg atcagtgtcc caataaactc tcccatgatt tgagtcatat
    79801 cactgttcat ttatttctcc ccctttagtt cccgcagatc tgattctgca atcaatgctt
    79861 ctaaacgttt agtctgttgc gccttctcct cttctgtcca ttcaaaatat ccagcaattg
    79921 catcgatcac aggttgcttg atctcatcaa gctgatctcg ttcaaacaac agatgatttg
    79981 tacgacgaat gagataatcg cctggggcca aaaccatttc ttcttctaaa ccataacgta
    80041 accgagctga ctcagcgaga ctcaaaccag gataaggtgc catttctttt gctaattcaa
    80101 agatacgtcg ggcattagtc ccgtaaaaat cagcgatata agtagcatct tcttcctcta
    80161 aaccggctgc tactccttct tttgctaatt ctgtcactgt ttcttctaat ttagttggat
    80221 cgaagtttcc acctgaaatc tgatattttt tagagtcgat ttctttcgtc tctattccgt
    80281 attcttcttt taataattgg cgtatcaatc gcaaagctcc ttctgccatc ttacggtaat
    80341 cggtgatttt cccaccagat aaagtcagca aaccatctgg ttctctttct aaagaactac
    80401 ctctggaaat cgtagaaggt gctttttcat cacggctgtt ttccaaatgg ttcaacacgt
    80461 cttctacttc agcacgagaa actttgtttt ccttatattc acttactgta tcgacaactt
    80521 tattgaagct cttgtctgaa atcgatccat tatctccacc attgtaatca gaaccagaat
    80581 taccaatcaa cagtggacga agtcctgccc agcttgcttc gatatcattc aatgtgatat
    80641 ttgcttctgg atagcgatga ttgatcacat ccaatagata atctacatct tcttgtgtga
    80701 ctttcggatc cgtaaaatct ccttggtaat ccgtatctgt cgtaccaaag taagtcttgt
    80761 tttctcttgg aatagcaaaa accatccgcc catcttgctt tcctgtatcg aagtatgtgg
    80821 gttgcggtac aggcagtttt ttcgcatcta caactaaatg gatcccttta gttggacgca
    80881 ttttaggaga gactggacgc gtaaaattca agtttcgtac tttatctacc caaggaccac
    80941 tcgtattgat cactaatttt gctttgattt cgatcacttc atctgtcagc aggtcacgag
    81001 ctttgacgcc aacgatttga tcgccatcat ataaaaagcc aaccgctttc attttactta
    81061 ctagataagc cccatcttcg gcagcctttt tgatattatc aatcactaaa cgggcatcat
    81121 tgttgcggaa atctagatag acacccgcgc cttttagccc ttcttttttc aaaaacggtt
    81181 ctctttccaa tacttcttct ggtgtcagcg tataattctc atatttggtt cctgtcacat
    81241 ttgccagttt gtcataaagg tccattgcta ctttgaccga gaacatattg aaggttgttg
    81301 ctccttcgcc ctcatagatt ggcaaaagca ttggatctgg ttttgggatg tgtggggcaa
    81361 tactttgtac gactgcacgt tcaccaactg tgtcagctac tacttccaca tcaaatgtct
    81421 tcagataacg aataccgcca tgaacaagtt tggtcgagcg agaggaagtc ccttctgcaa
    81481 aatcctgcat ttcgatcaat cctgttttga ttcctgatgc tgctgactgg actgccacac
    81541 cggcaccagt gattccgcca ccgatgatca acagatccaa ttcttcttgc tgcatttttt
    81601 gaatgctatc ttgtcttgtc ttgtttgaaa acatatggat ttcctcctta ttcaaatgct
    81661 tgggtagctt ttactgcttt tttccaaccg ttgtaaagtt tttctcggcg ctcttcctcc
    81721 atttgaactt caaatagctt gccagcttca taaaattcgc ggatctcgtc tgtatctttc
    81781 caataaccga cagctaatcc tgctaaaaat gctgccccta gcgccgttgt ttccaagttt
    81841 tctgctcgtt ggatcggtac attcaagata tctgtttgga attgcatcag atattcattg
    81901 tttgctgcac cgccatctac tttcaataca ggaatcttga ttccagtgtc atcttgcatc
    81961 gtatcgatga tatctctcac ttgataagca atcgactgta aagtagcttt aatgatatct
    82021 tcttttgtcg taccacgtgt caagccaaac atagcccctc gtgctgcctg atcccaataa
    82081 ggagcaccta gcccaacgaa tgcaggaaca acataaacct catcttcgct tgtagagcgt
    82141 ttcgctgcgt cttctgaatc gctagctttt tgcaacattt gaagtccatc tctcagccat
    82201 tgaatcgaag aaccagccac aaaaatactt ccttctaaag catagtaaac ctttccattt
    82261 atgccgtaac caattgtcgt caataaatta tttttggata attgcggttt ttcaccggta
    82321 ttcatgacaa taaatgaacc tgtaccataa gtattcttga ccatgcctgg ttcgaatgcc
    82381 atttgtccga ataatgcggc ttgctggtct cctgccatcc ctgaaattgg tgcttcgctt
    82441 ccataaaaat gataaccttg cgtataacca taaacttctg agttagaagt gacttttggt
    82501 aacatcactc gaggaatatt tagtaaatct aaaatctcct gatcccaatc tagctgatgg
    82561 atattgaaca acatcgtccg actggcattc gagtaatcag taacatgtgc ttgtccatcg
    82621 gtcagtttcc atactaacca agaatcgatc gttccaaaca tcaattcgcc tttttcagct
    82681 cgttcttgcg cgccttctac atgatcaaga atccagcgta tcttcgttgc tgagaaatag
    82741 gcatcgatca ctaaccctgt tttttcgtgg aaaaattctt gatagccttc attttttaag
    82801 ttatctgcaa ttccagctga ctgtctggat tgccaaacaa ctgcatgata gattggacgt
    82861 cctgtatctt tttcccatat tactgttgtt tcacgctggt tcgttatgcc aatcccagca
    82921 atctgatttg gtttgatccc tgattcgatg aacgctccgg caataactga ctgtacagaa
    82981 ttccagattt catttgcatc atgttccacc caactttctt gcgggaaata ttgcgtgaac
    83041 tctttttgag aactgccgat gtgtcttcct ttcttgtcaa agataattgc tcgtgagcta
    83101 gttgtacctt ggtcaatcgc catgatatac gttgattctg tcattgtgat tccctccatt
    83161 atatgcaaac gctttcttta actactctta tcatactact gttttaaaaa aagaaactgt
    83221 caaaatttta ttcttttttt ctttttttga cagtttttta ttatgaagct attttttaat
    83281 caattctttt cttttttcag cgtctctatt cttcttttca atcgaataat gtcgtaaggt
    83341 gacgcaaatt ctgacagata atattcacac tgttttttgt ttctctggca aaatgtgatc
    83401 accaaatcat attctttacc ttcttcataa cttccaatct caataccgcc gattcccctc
    83461 agttctctga tcagatattg ttgaaaagca atacgataaa tcggcagact ttctaaatcg
    83521 attccaatca ctactggttt tgcaatataa aaatcgatca tcaaaagaac attggcatac
    83581 tggatcatca ggtaactaag ctcactatct tcagctcttt tcctcggcaa ttgctgcacc
    83641 gctgtttcct gtaatttcgc cagtaactgt gttaaggaat gcccgagcat tttctgctgc
    83701 tgttccaaaa gccgatcacg ctcatagact tcgatctttc caacaaagaa aaaccattca
    83761 ttgttgatct gggaaatttg gtagcctact gccttttctt ctttgatttt caaacgacga
    83821 ggacgataat gccataacat cgtttcccga atgtaagtat ctaaaacagc ggttggtaac
    83881 tttttgctcc gtgtcaaatc atactgataa aatatttctt cgtctactat cgaaaaagaa
    83941 acaaaaaagc tgtaaaacat cattgtttcg tatacattca gttctgctgc tgttcgacta
    84001 agatgcatca cgatgattgg atcgattgct tgataaagcc ggtccgactg atagagtaat
    84061 ttcttttctt tcaaagttgc gtacgtactt ttttcattca atagtctttt tttcgtaatc
    84121 ccgagccagc aagcaatctg tgcttctgct gatgccgtaa acgttgtttc taatacacgg
    84181 tccaatccta gaatgaacgg tcttttttct ggtgtatttt gcaaaaaaag cggtcgctga
    84241 tcttccggga tatattggaa tagttcataa taaaaatatc gtatctgtag ctcttctccg
    84301 tataattgac cgtttttgat ttgtaatcca aattcctcta agagctggtt caattctttg
    84361 atctttcgaa aaacagaaga ctcactgata ttaaacgctg tcgctaatcg aacgatggat
    84421 acttgctgat gttctaataa atagacgagc atcttaaact tcaagctttc ctgcaataaa
    84481 aacaatagaa ttttttccaa acttacatct tcgtccaaca caagcagcgc ctgatactca
    84541 tttcgctgta cttctacttt tttttccatc attcggccaa tcaattcaat atcttccaaa
    84601 tattgatcaa acgaggcttt tgacagttgg acatgctctc tcaaaccatt gatagaggct
    84661 gttccccctt gcagaacaag gtttcgcaaa atcaggatct gtaactgctc ttttctttct
    84721 aataatgctt caatcttcat aggcagtatt cgccaccttt gaccacacca tatctggcac
    84781 acggtattct ggctggaaaa atagctcatg ttcttctaac tgttcaaacg tccatccttc
    84841 ttctactgca attgtcagtt ggtcaagaag gtggaacatc tcttttttgc tttggagctg
    84901 tgctccaaga atcttttttt cagaatcaaa tagaaatttg agtttaactt tgctttgatt
    84961 tattgctgta agcttgtagt ctacgtatcc tgtttcaatt ttttggggat aaaaaatgct
    85021 ctcttcttct gtcaaaccta cgcttcctaa gaaatagcca aaccactgat tgccgactgg
    85081 acgataggtt ccctgatcct ttgtctgtga gccactaatt gtcttgctga catttcgtgc
    85141 tgtacggaca gcgttattta ctagagaaac atacattttt tcatttgttg gacgaaaagt
    85201 tacttggatc gcatcgccaa tcgcataaac gtctttttga gacgtttgca agtagtcatc
    85261 tacccagatc gttccatcat cattcaatgt taattgttct tcccacatct gattgtcggg
    85321 gcgagaatga ttagccagca aaacaagatc atagttctcc tttctttttt ccgtctctac
    85381 aatagcggtt tcattttcgg tcaaagacac aacttgttct tctaaaaaaa gtgaaagccc
    85441 ttgatttttc atttccttta ctaacggttc tgtcatttca ggatctagat aacgaaaaag
    85501 aattgacttt cggctttcgt aaagatggat ttgttttttt cttgatgcca acccttcagc
    85561 tatttcgatt cctgcttgtc ctgcaccaat aattgcaatt ttctttgcat tcatgatctt
    85621 ttgctgaaga gccgttacgt tttgtatctt tttaactggg tgaataagcg ttgattctgc
    85681 agaaatattt cgaaactttt gatttgaacc agtcgctaga atcaaacgat caaaatccaa
    85741 tttttttcct cctgctaaaa cgactttttt ttctttcaat cctattactg tcttttctgt
    85801 gtaaacatgg attcgatagc ggtcagtcag ttcttctttc gtgatccaag aaagttgttt
    85861 ttcactggta acctttcctt ttaatatcaa attgaacccg ctgggaatcc atcctaactt
    85921 cgattgcttt tcaatcaaat atatttcact tgttggcatc aattggcgaa gataaatagc
    85981 cgcatgaatc cctgcaaacg acccgccgac aattacaatg ttcatctgca ttcctctttc
    86041 ttataaggtg ttctactgct aacttctcaa tcagaaaaca cagtattcat aactcgcttt
    86101 caaccttatg atacatagcg atgcagcaga atcaaccctc tccatcatac cgtgttctag
    86161 gtctgcttct caataatgta ctagtggacc taattgaaga gtatcatttc tgaagagatg
    86221 tttcaaaaaa tgtactctta taaaagaaaa aaactcgcct tttacgtacc tctgtccaaa
    86281 taggaaacag aaaagaacga aaagacgagt tttagcgtgt gtaatgtaat agttagaata
    86341 gacacatcat ttatttttaa ttctttaatg cttcaacaac tgcttcgcct aattgatgag
    86401 cagattgagg attttgccct gtaagaagcc gcccatcaat ttgaaccact tctgaaaaag
    86461 ctgctccaga ctgatagttt gctccttttt ctttcaaagc atcttctgct aagaatggta
    86521 cttcattcgt tgtaccattt gcttcctctt cttcattact gaacccagtc actgtacgtc
    86581 cagctaaaat agattttcca gactcatctg taactggcaa caatcctaca acaccgtgac
    86641 aaacagctga gaccagccct tcatttcgat agatttgtga agcgactttc ccaagatcta
    86701 ttgcttctgg aaaatcccac attgttccgt ggccaccagc ataataaacc gctgcataat
    86761 catttgcatc cacatcatca ggacgtaagg tatttcccaa ggcccgattt ctgaaatctt
    86821 catcttcata ataggcccaa tcgatttcat ccatattcaa aacactttgc ggttctaaag
    86881 gtacatatcc gcctttagga ctgacaaaat ctgcatcgat cccatttttt actaatacat
    86941 cataaaaatg agtcagttca cttaaccaca aacctgtagg acgctcgatc ttttcgtact
    87001 tttctacatt tgttaatacg atcaatactt tcattttccc atcctctttc tataaaaatt
    87061 gcgttattga taacgtgctt tcaagaaatt atataatggg cttggcaaat aaagctcttt
    87121 atatgcttct tcaaaaggtt tcgttacaaa ttcatcctca cggttttcca ataatttctc
    87181 tgcccagtca ggatcgacga tcatttgctg tccaattgct gcaagttctg catatttcaa
    87241 tacctgctct acatcttcgc gattgcggat cccaccgaca ccaactaaag ggatctttcc
    87301 ttgaatcgtg ttatggacca cttcaagaat cgatttatcg ttgtatttat cagaacgagc
    87361 tattcgatca tacatattta atgaaacatg cagataatct agttttgact cacgtaatcg
    87421 ttcaagaagc cagatcgtgt cttcaaaacg gatacctggt gtttcatatt cttctggtga
    87481 gaaacgatac cctacgatga atggtttttt agcatattgt tcaaccgctg agaaaaccgt
    87541 tgttaataat tcttcaataa aacgataacg attttccaag ctaccgcccc attgatcctc
    87601 tcgtctattc gaatgcggag agaaaaattg ttggatcaaa tacgtatttg ctccatgcaa
    87661 ctcgattcca tcaaatcctg attcaatcgc tctacgtgtg gctttgccaa atgcttggat
    87721 tgtttcaacg atttcttctt cactcattgc tcgtggtgtt tccgcatcag gacgttttgc
    87781 tgcaactgca cttgcagata caggctgttc tccagataaa gttgctcgac ttgtcattcg
    87841 tccaccgtgg aaaatctgaa taatcgcttt tgcgccttct ccttggattg ctttcgctag
    87901 ctcacttaat cgaggaatca tttcatcatg agcgatgctg agttcacctg gccagccttt
    87961 tccgatatct tggacatttg cagcaccagt aataacagca ccaagacctt tagaacgttg
    88021 tttataataa gtgatctcat cttcggtgat cactccattg aaaaagctga gttgtgtcgt
    88081 cataggcgac agcatcaatc ggttcttcaa ttgaactcct gaaggtaaag taattgattg
    88141 atcaaattgt gacattgtca ttcctcccat aattcgctct gattaaccta tcgagaatca
    88201 tctatcatta aaccactcga ttagttgtac atgcaactaa tttgcttaaa aaattttatt
    88261 ttttttgtaa cttatctgtt gcatcattca ttgtctgggc taattcttta cttgcttcat
    88321 ttcccaaaac tgctttgtat gactgatgaa gttcttccca gctaccatcg acttccttag
    88381 caaattggcg tccttgttct gtaagataaa ctaatgacaa gcgaccttcg ctaactgttt
    88441 tgacgtatcc tttatattct aatttttcaa caaaacgagc aatcgttgat ggggcggaat
    88501 agagcatttg tgcaatatct ttttgtggta ttccatcttt ttcttttaat atcaataaaa
    88561 taaaaccata agttggtgcc agacctgtcc ttctaaacgc ttcatcagct aatttattca
    88621 acatgcgatc tagctttttc acgctaaaga aaagacattc atctaacgct ttttcaattg
    88681 gcttttccat ttcatcccct ccattggcgt acatatcata gtatgatttt tattttttgt
    88741 caaaaaacac ggaataatgt ccgcctcctc tgtctagatt tgaaaaaaag aagtagccat
    88801 cttcaaagaa tttcggcttg ttttcgacaa aactccttct ggaacaccgt gaatcagagg
    88861 ttgtgaaaaa gtcgtttagt tttgagtatg aagaaaaatt ttgtgaaaat ggctttccac
    88921 atttttgcat aaatttggct taataccgaa aaactggctt ttgaacaccg tttatatcag
    88981 aggttgtgaa aaagccgtcc tgcatcaagt aataagaaca gccaaacaaa aatagttcct
    89041 catattttgt cgttttttga gctaatgccg atggtcagct gattgtacac cgtttattga
    89101 gaggtcgtga gcctgccgtt gaactccgag tcacaaggag caatttcaaa aaacagcttc
    89161 tcatgtttta tgactatggt gacttgtgat cgaggagttg gcaggagaat actgtttatt
    89221 cgtaaagaag gggctgtgac aagacttttg tcacagtccc ttatttcctt ttattatagg
    89281 atttcgccat ttgattggat gacttgtctg taccaatcaa atgatttttt ctttaaccgt
    89341 ttctttgtgc cattcccttc gtcatcttga tcaacataga tatatccata acgttttgac
    89401 atttgtgaag tagaagcgct gacgatatca atgcagcccc acgacgtata accaaataaa
    89461 tctactccat ctttgatagc ttccttcatc tgctcaatat gctgtttcaa atagtcaatg
    89521 cggtaatcgt cttgaacggt tccatcttct tctactttgt ctatagctcc gagtccgttt
    89581 tcagctatga acagtggttt ttgataccta tcatataatt gattcaaagc aattctcaaa
    89641 cccttaggat caatctgcca gccccattca cttgatggaa gatacggatt tttaattcct
    89701 ccagttatta agtttccttg gaccccttcc caattttctg gatgagcact gactgcacta
    89761 gacatgtagt agctaaaaga aacaaaatca acagggtagg ctgctaagat ttcttcatca
    89821 cctacttcta tttttaagtt gacacctttt tcttcaaaca ttcgttgcgt atacgctgga
    89881 taatatcctc ttgcttgaac atcactaaag aacaaagaag ttctttgctg ctcaattgtt
    89941 ttttgaatat catccggatc acaagaatac ggaacaagaa gctgatagct gatcatacaa
    90001 ccgacttgtg aacctttgat aatttcatga caagcttttg tagctaaagc acttgctata
    90061 aactgatggt gtgcaccttg gtatttcgct tgttccaaat ggggatggtt ttcttcaatt
    90121 actcctacgc taacaaaagg gtgatgtttt acacagttga tctcattgaa agtcatccaa
    90181 tattttactt tatttttata gcgtttgaat actgtttcag cgaaacgaac gtaaaactga
    90241 ataagctttc gattaggcca tccaccatat tctgtaacca aatataacgg catttcataa
    90301 tgggaaagag aaacgactgg ctcaatccca tattttagac attcgtcaaa cacttgatca
    90361 taaaaaaata atcccgcttc attgggtgtt tcttcatcac catgaggaaa aattcttgtc
    90421 catgcgatag acatccgata gcatttaaaa cccatttcag caaataaacg gatgtcttct
    90481 ttaaagtgat gatagaaatc atttccgtgt cttttaggat aataatactt gctgttggga
    90541 tcctgcgccg cttttacttg ctgatgcgtc atcatatgcc attgatctgt ccatttttct
    90601 ttaggtaaat cgctgtcgaa tgtatacaca tcagacacag ataatccttt ttcatctaca
    90661 tcccatgcac cttcacattg attggccgct gtcgctccgc cccataaaaa tccttctgga
    90721 aattttttca tatcgatccc cctaaacttg ctagttttct tgtttgatta tttttcttat
    90781 gactagtaat gatatttttg aatctactac tatttgtaac aataaccatt gtggtcggat
    90841 catatccctc tttgattaat tcttgcaaat ctacttccat caataaatcg cctttacgta
    90901 ctttttttcc atcagatact gcactttgaa agtatttgcc atttaaattc actgtatcaa
    90961 taccgatatg gatcagtatc tctgctcctt cttcactgac taccccaatt gcatgtttcg
    91021 ttggaaaaac tgttgaaact tccccgttta caggactgta gatttttccg tccgttggaa
    91081 tgatcgcaaa cccttctcct aaagatccac tagcaaatgt tggatcgttt acttcagata
    91141 aatcaattat ttcacctgct gcaggagtac ttaacactat atcctttgag gattcttctt
    91201 cgatttcatc cttgtagccg aaaagaaaag ttagcataca ggaactgatc atcgctaaga
    91261 aataactagc aactagccaa acaaatcctt caccaatata aatcgggaaa gacaaaatac
    91321 ttcttgtagc aactgccaaa gcaccggctc ctgcggcacc agctactcca ccaaatatag
    91381 ctccacatat acaagcaatg taaaatggtc gtttatattt aagagtcaca ccataaacag
    91441 caggttcagt gattccaaat aatgctgtga taaatgcaga taaagataaa ctttttactt
    91501 tcttattttt cgttttcaaa aataccccaa gtactgctcc tgcttgcgca gctacggcag
    91561 tcatacatgc tggacctagc gtatcacgac cgtagaaaga aagattgttg gtcattactg
    91621 gtacaagtcc ccaatgtaac ccaaaaatca ctaacacttg ccaaaatccg ccgataacag
    91681 caccagataa aacagggcta aagttataaa cagctaaata tccagctgca atcagctgac
    91741 ttgcataatc tgcaattgga cctacaacta ataaagtaat tgggaccatc acgatgatac
    91801 aaatgaatgg tgtcaataaa tttcgaatac tttcgtgcaa gcttttgcga atcttaggct
    91861 caatataaga aagaacccat acagctaaaa ttgctggaat aaccgtactt gtataccttg
    91921 ctagaatcac gggcatccct agaaatcgca aggttatgct atcactatat gcactgacaa
    91981 tcgtaggata aacgagagct gctgcaataa ccatagcgat gaaacgatct gtattgaact
    92041 tttttgcagc agtatatgcc aaaaatatag gcaagaaata gaaaaatgca tcagctgctg
    92101 cattcaaaat gatataagtt cctgattcag tagttaataa attcaaactc gtacataagg
    92161 cgagtactcc tttgatcaaa ccacttcctg ccaagacgcc taaagtcggg gcaaaaacag
    92221 cggcgatcat gtctataaaa gagtttaaaa tgcctttttt ctcagttcct ctctttccac
    92281 tagatgcatc atcttctagt ttcatttgtt tggataatac gtcatacact tcatttactg
    92341 catttcctac tacgacttga aacattcctc cagcttcgac tacagtgatc acaccttcta
    92401 gttgctctac cttctcctta tctgccttct tattatcttt tagggtaaag cgtaaccttg
    92461 ttgcacaatg gtaaacattg ctgacatttt cttcgcctcc gacaaatcgg acaatatctt
    92521 tcgctaattg tgaatagtcc atttttactt cccccttgtt aattaatgcc gtagcaatcc
    92581 ttcttacaag ataagtatag ttcttagaaa acggtttctc cattcaatta gtacccctta
    92641 taatagcgaa cgaaactgta caatagtgtg tatggtttac ttagccgagc aaatcactca
    92701 ctgcttgaaa gaaaacaagt atatcgtttc tcttatagtc aaaggagaca aaaaaaacac
    92761 gcttgatctt cctttgctga tgtataagtg gtatctcaag ctgatactct gtgatttttg
    92821 tttctacttg gcttttgata agacagcaca acaaagtctc ggcatttaac ggaagcataa
    92881 ctaggtcaga actaggagag attagttgct gaatggaatc tatcttttta tggattttta
    92941 tttgattttc ttctttgtag taatgatcaa gcagttttgt tttctttttt tcttcaacta
    93001 tggtcagctt ttcacgcaaa atctgataaa tctcccaaga ttcaaactca tctagaacac
    93061 taggtagata aatcaaatca tcccactcaa aaataagctc ttgctcagta gtcaaaattg
    93121 catcatattc cgttattttg tttgattgat ttttgaaaag atacactggt tctatccggc
    93181 attctttgat ccacatgttc atactttgtt gaatctgttt ttttagtgta ttcaaaatac
    93241 taattggctg gttactaaca atcagtatct ccccttgttt tcgaaaagga ttcaacgctt
    93301 cggcaagata caaaacaaaa taagcgcttt cagctaaatt gggacgtaat tgaaaatgta
    93361 ttgggaagaa acgccgaatt aaataaattt ctaaaggata acgggagatg atcttttgta
    93421 caaaatgatt catggtatta tgtccggcat ccatcctcgt tttcatttgt ttgatatggg
    93481 ttagcaaaaa ttctgtttca tggaacaata acgaaacagg aagatctaaa aaacgaataa
    93541 tcgtttgctc aaatgctctt agattcttct ctatttcttt gtttttttca tcattaatca
    93601 tcaaataatt taactctaat agtcgatttt caattgcttg tttttcttga ttggaaaaag
    93661 aatagggtag ttctgatgat aacttttttg tgagggtctc taccatcgga ataaataatg
    93721 tatacctgtt tgttttttct aaaagttctt ctcttcgaag aatggtcaaa acgagatacc
    93781 gagaaaatcg tatgaagtcc tctcctgaaa caatataatt atatgatata agaacctctt
    93841 ttaatgtctt ttggacagtc ggcatgatat tggaaaaaat tgtactgatt tgttgaggca
    93901 accttgcgtg ttggatcacc gtttctgttc caacaagaga taagaaaatt ctttttgaat
    93961 tttcttcagc aatgattttt agtccttttg tcgacgaaac ttccagttct aatttaggat
    94021 tgcgctcgat ccaacgcaaa atcgttttta tttgtaatga tacggctgtt tttgaaagaa
    94081 agaaaatgtc tgctatcttt tgcatactga catacccttt ttgaaacaat aagtacgtca
    94141 caatagttga agctcggaaa tccatgctgg aatagatttt atgtttcgtc acctcttgta
    94201 tgatttgctg tttagtttca gattcaattg attctaaaaa ataacctgaa cgtttatttg
    94261 actggataat gattttcttc tcgtagatag caagcatatt tttgatttca gtttggatcg
    94321 ttttcttgtt ggtacctaaa gcttcagcta attcttcgct tgttatccaa tgattcttat
    94381 ttcttaatac tacaagaatt tggagttgtc gtttatttaa gtccatttgc ttacacctcc
    94441 tctttgttat aaacatacag gaaaaacctc tcaaatgaca atagtcactt gcagaggttt
    94501 aaaaatcatt tgaagaaaga tctatattca aggtttttac cattagactg gataatttgt
    94561 ttataatatt caaaactatc ttttttcaat ctcctaaagc ttccgctccc atcattttgt
    94621 ctatccacat aaataaagcc atatcgcttc ctcatttccc ctgttcctgc cgatacgata
    94681 tcgattggtc cccaccataa gtaaccaatg atttggcaac catcttttat tgcctcagca
    94741 atttcctgaa gatgtatttt tagatactca caacgttttg gatcgtggat tcgattcaat
    94801 tcatctggtg cttcatctag tccaatgcca ttttcaacta tgaataatgg taattcataa
    94861 cggtctgtca attcattcat gacataacga aaccctagag gatcaattgg ccaactccaa
    94921 ggttcgggag atttttcctt caaaaaggga ttatcgaaac catttgctcc gccagtatct
    94981 acgcgcattt ctgcttcttt tttatagaca gcgctacgat aataactaaa tgcaatataa
    95041 tctgccgtgt tagcttgcaa gcactgttcg tctccatcta acattactgg tgcacagtct
    95101 tcttccttcc atgtcctgta aatataagct ggataatgcc ctcggcacat gacatcggag
    95161 aaaaaccatg aattgcgttt aaattgcagt gaaccaaata cattctctgg atcacagtca
    95221 tatgcataag tcgctaatgc agaaaagcta cacataatac ccatcttagc atttggatcg
    95281 attttcttta ataattgagt agcacgggcg ttcgctagaa acatatgatg acaggcttga
    95341 taaatcactt tttttgttag tgtagcaatg ggttcaagtt tatctctagc agggaatgta
    95401 accaatccag cagctgcata aggcatacga aaagcattat tgatctcatt aaaggtcatc
    95461 caatatttca ctttcccttg gtagcgttca aagacggtct gcgcataaaa ttcaaaaaag
    95521 tcgattaatt ttcgattttg ccacccccca tactctccta ccaatgctaa gggggtttca
    95581 taatgactaa gagtaattac tggctcgata tcatatcgat ttaatgtatt gattagtctg
    95641 tcataaaata cgagaccagc ctcatttggc ttctgttcgt ctcctcttgg aaatattcgt
    95701 gcccatgaga tgcttgttcg aaaagctttt aaacctaact ccttcagtaa acgaatatcc
    95761 tcttcaaatc gatgataaaa atcaatcgct tcatgagaaa gataatggag tgattcgtct
    95821 aaagcaatct cccaaatctt tttttcttca ttccactgaa tactaggatg ctggttcatg
    95881 atcccgataa gcgtatctgt cacattaggt aatttgccgt cttccaacca tgcaccttca
    95941 cattgattag cagctactgc tccgccccat aaaaaatttt ctggaaatat ttcctgatcc
    96001 ataactatct ctcctcgcta tcaacaattt cttacttgaa gtatagctcg attcatcaaa
    96061 acaagatatt ctaactattt cggcagaaga gaagaaaaaa attgaatgct actcttcatt
    96121 tttaatattt atatataaca aatacataag acgatccccg aaagaaaaca ctcgttaacc
    96181 atctaagcaa acgatttatt ttacttccaa cacaaatgct tcataaggtc gtaaatcaac
    96241 tttgccgtct ggtaactgtt tcagaggata atttgacaca ataaccctta aagcttcacg
    96301 tggaacagtg aacgtctgct tttcactgga catattacaa ataacgagcc attgctggtc
    96361 ttcatatatg cgagaatagg caaagacatg ctcaggcgtc ttttccacta attcatagtc
    96421 tccccagacg atgatgtcat gttttctacg caaatcgatc aatttcttgt acatataaaa
    96481 aacagaatct tgatctgcta acgcttcttc tgcattgatt tctttataat ttggattgac
    96541 tcgaagccat ggaattccag tagtaaaccc agcatgttca cgggcgttcc attgcatagg
    96601 cgtcctagca ttgtctcttc ctttcgcatt gattgattcc aagatttcct cttttgtaaa
    96661 tccagaagaa atcctttcgt tatacatatt gattgtctca atatcttttg cttccgcgat
    96721 atcatcgatt gggcaattcg tcatgcctaa ctcttctcct tgatagatat aaggggtgcc
    96781 tttcatcata tggagcaaga tagcaaacat cttggcactt tcttttcgat acacatcgtc
    96841 atttccccaa cgagaaacaa ttcgcggaag atcgtgatta ttccaaaata aactattcca
    96901 tccttcatca cctaaagatg tttgccattt ggataatact tttttcagtt cagagatact
    96961 caagggtttc aaatcccatt tgtctttgcc ttcttgctga tctaatccta tgtgttcgaa
    97021 ttggaagacc atggataatt catttctttt aggattggaa taaagtttag cgatttcggt
    97081 agttgctccc catgtttcgc caacagtcat cagatttttg tcaccaaaag ttgcttgatt
    97141 catttcctga agatactcat gcagtttagg tccattactt gtgatttctt tatccggttc
    97201 tttgccaatc atatcgatca cgtccatacg aaagccccca atacctttat cgatccagaa
    97261 attcatcatt tgataaatct cttggcgaac ctgtttattt ttccaattca aatcaggttg
    97321 acgcttgcta tacaagtgca gaaaatattg ccccgttttt tcatccaact gccaagcaga
    97381 accactgaat attgagcgca atctattagg ctcttttcca ttgaccggat cacgccaaat
    97441 ataatagtcc cgataaggat tttctttatt tttacgggcc tcaatgaacc acggatgttc
    97501 atcagaagta tgattcacca ccagatccat gatgatacgg atatttcttt tctttgcttc
    97561 ttcaatcagt tcttccattt cttccatcgt accgaattct gccatgattg cttgataatt
    97621 actgatatca tacccattgt catcattcgg agactcgtat actggagaaa gccaaattgc
    97681 atcaatacct aaatacgcca gataatccaa cctggaaata atcccccgca aatctccaat
    97741 accatcttga tttgaatctt gaaaacttct tggatagatt tgataaacaa ctgaatggtg
    97801 ccaccatttt tgttccatga ttttcctcct ataaatggat gtttctattt tttgcttaat
    97861 catttatttt agtctctctc actaaaaatt gtaacgcttt caaatagcta ttgcaccttg
    97921 ttatcggtaa cagaataaaa caaaaaaaca gctgaggaaa ttccctcaac tgttttttga
    97981 aaggattgat ttatgcaggg aagaatgctg atttcacaac ttctttataa acggtgttca
    98041 ctcagctgtc ccttggtatt agctcaaaaa acggcaaaat atgaggaact attttttcgt
    98101 ttttttctct aatactcagg ccagaacact gatttcacaa cctctttata aacggtgttc
    98161 actcagctgt ccctcggtat tagctcaaaa aacggcaaaa tataagaaac tattttttcg
    98221 tttttttctc taatactcag gccagaacgc tgatttcaca acctctttat ttcacattca
    98281 atatccacat gaatcccaca aatacaaaga acagaccata catgattgga gatacttctt
    98341 ttcctctttt agctgctaac atggtaattg gatagaagat aaaacctaat gcaatcccat
    98401 ctgaaatact ataagtcaaa ggcatgccga tcaagatcaa gaatgaaggg atcgcaatct
    98461 ctaagtttcc ccattcgatt tgtcgcaaag attgtgccat caatactcct accataatca
    98521 atgctggtgc agttacttgt gaagtaacaa ctgatagtaa tggagaaaag aacaagccta
    98581 acaagaagaa aatccctgta gtaatagctg tgatacctga acgtcctcca acggcgatac
    98641 cagctgatga ttctacaaat gcaccaacag gagaagtacc aaataaggaa cccgcaagca
    98701 tagctgttga atcagaagct aaagctttcc ctacacgagg catcttgttg tctttcatga
    98761 aacctgcttg gtttgctaat ccaactaaag ttcctgctgt atcaaagaaa gtcacaagca
    98821 agaatgtcag cactacgacc cacatttgaa gagaattgat gtcccctaca tgttttaatg
    98881 caactaaaaa tgttggtttc aaacttggtg ctgcggcaac aatttcagaa ggcattggaa
    98941 tcaaacctgt gaccagacct aaaatcgttg tagctgctat cccgatgaag atgccacctg
    99001 gtacacgccg aacaagcaag atagcagtta cgactaatcc aaaaatcgtc aaccaagtag
    99061 aaccaacgtt gagtgatcct aatcctacta atgtagattc attcgaaacg atgatcccac
    99121 cttcgctcag accaaggaaa gcgataaata gaccgatacc gccagaaatc gcatatttca
    99181 aatctgctgg gatcgcatca atgatcattt cccgcagctt gaagattgtg atcaaaataa
    99241 aaatcaaaga agcaacgaat acgccggcta atgctgtttc ccatggaatc cccattccta
    99301 aacaaacaga ataagcaaaa aaagcattga tcccaagagc aggtgccgtt ccaattggat
    99361 aacgggctaa tacccccatt aaaacacagc cgatcgcact ggctaatgca gtcgcagtaa
    99421 acactgctcc ttcatccatt ccagaagcac ctaatacagt tggatttaca aataagatat
    99481 aagccattga gataaatgtt gtaaatcctg ccagtacctc acgttttacc gtggtgttca
    99541 atttttctat ttcgaagtag gaaagtattt tttccttcat cttccatccc cctaaatccg
    99601 aacattataa ataaagtccg ttaaaatttc gatttattaa taaaacaacg tgttttatca
    99661 tatactaaaa caaaacataa gtaaacaaaa aattcggatt ttaagcaaaa tccgaacgtt
    99721 ccatctgatt aaatcccttt atctcaaagt tcatgaacat tcactttgga ttacaagaat
    99781 ttctttattg tgttaataaa gtttcttcat tctagtctca aaaatcaata gtgtgatttg
    99841 attttaccat ttcgtttctg ttcttctttg atgaattctt ttgtatcgac aggattcaga
    99901 ttatttagtc cgccaaaacc tgtatcgacc atttttcccc agccttttgt cttctttttt
    99961 cctgatttga gattctttgc tcgaagcttc tcactatgtt ttgctcgccg atacttcaaa
   100021 taaccttcta atcttcccat tagatccact gtttttcctc ctttttattc aatcagacga
   100081 ctcagctttt cctataacaa aataattgcc ttcagggtca gaaaaattga aagtcaagat
   100141 gccattgtta tctgtgattt ctcctggttc ttccatgttc tcgtagatag tttcaaattc
   100201 atccgtaaaa aatactaaag aaggcggtgg tcctaatacc tctggtgaaa acttctgcac
   100261 aaatactttt gggaatacgc ctaattctaa ttcttttgag atgctgagta caatactctt
   100321 aaattcttct ggcaattcga ttgtttcaga aatttctgca ccaatttttt ctacaaagaa
   100381 tcggcaaatc atctctacgt cattgacata aagcatcaca cgtgtttttt gtatcatcat
   100441 ttgacccttc tttctatttg attttctttc ctgtctataa actgattcca ttatatcacg
   100501 aaagcgcaaa caccttgtgt ttgatactat tcaagaaaaa taaagcaata gtttctagga
   100561 ttgctttcct ccacaaactt tttttacaaa aaaaaaaaac ggcaatgaat gccgctcttc
   100621 ttatttcgat tcttttataa ttctgcttct gattcatctt tggttagtgt gtgtctaaca
   100681 ctagttacgc caaatgtttg atcgattttt tccttcaacg cttcgattgc ctgtgcgcca
   100741 cgtgccgctt gttcagcatt gatggattca atgaccagca ccgctgcttg tgtttcttcc
   100801 gtcagcattg ttctttgggc ttcacgccag ttgagacagc gacagatcgc tccttgttca
   100861 tcatagtaga tgatttcttc cggaagtgcc ggagcatcgg aatctgcacc taaaggcagg
   100921 aatgattcgc caccttgggc ttttcctaaa tgcagatttc ctgcgatctt ttcgatatct
   100981 tctccgccgc aaggtacgcc aaaagacaag gaaatactat tatagatatc tacaagcgga
   101041 ttgattggga aaaattcccg ttcttgattt gcacgtttca gtaaagcttc aatagaagaa
   101101 cgtgcacctt ttttagtctt gaatgtggta aatgcctgac gccactgagc aatgacttcg
   101161 ttttggctga atgtttcttc tgtaaggtaa tttcttgctt cttttgctgc ctcattcaat
   101221 aattgatgat acgtttcttc actttctttt gttacgtggt tatcgatacc atttacggat
   101281 aagatttcga tggttgcttc aggaaatact tcccagaaat tggtatccac tattactttt
   101341 ttcatctgtt cacacctctt gattagaata attgcttgat gaatagatag tctgtttggg
   101401 gatcatctcc catataaaaa acatgttcgc tgtatttgat aaatcctttt ttacgataaa
   101461 aagcgagcgc aacatcgttg tgttcccaaa caccgagcca aatggctgtt ttttgctttt
   101521 cttttgcttt ttcaatagct gtatctagta aaaatgtacc gataccttga tgtttaaatt
   101581 tttttcgtac atagattcgc tcgatttcta aaaaatgatc agccatttcc tcagattgac
   101641 tcgttcctgt atttagtttc aggtaacccg caggctcttg atcgatactc actaggaaaa
   101701 aatcggtttc tttatctttt agctcttctg taagtttttc tagtgcataa gcttgtgtca
   101761 agtaatcagc taaatgctcc ggtgaattat cttttccaaa tgtatctgta aatgtttcga
   101821 tactaatttg ttgtagtaag tctacatctg aaggcgtcat ttttcgaatt tcgtatttat
   101881 tcacggcttc ttcctttcaa tagatccttt tattgccttt tttgacaaaa ttccaatctt
   101941 cgctaacatt cttgcatact ttatttaaaa gatattccaa ttgttttgct tcttcgtcag
   102001 aaagaccatt taatgcgact gcattggagt agttgttttc tcgaatgatg aaaggataga
   102061 tttctgctcc ttttttagtc ggatagagtt tcttgatttt tttattttct ttatcttcca
   102121 aacgttcgat catcccattg gattctaatt tcttgattgc tcgggcagtc gttgtacgat
   102181 ctactttgat catttctgct agtttttctt ggatgattcc aggattttcg caaatacgta
   102241 ctaaataaag atactgtcct cttgttaaat cgacttcttt gaattcgata ttgctaatag
   102301 aatctagcgc tcgggcaatc attccgatgt ctcgaaggat ttcagccatt tcatcccacc
   102361 tttattttgt ttgtaagact agtttacctc tttttgttgc aaatgcaact attttcaata
   102421 aaaaaatcaa gttctttttt aataactagt aaagtatatt cttttcttct tttacgaaaa
   102481 agctcaatca caatagatct ggtttttgtt ttctaaaata agtaactgtt tcttcgttgt
   102541 tcgcttttct gatagctttg gcaactcttc tatactaaaa aatcccatgt tggaagtttc
   102601 attattctca ataaacttgg cctcttcacc gtggatcgca caagcaaaaa caagtttata
   102661 atactgaaaa agctgtggaa tatctttttg tttattcgta tcaaaaactg ctcgcaactg
   102721 agtagtttct actgttaatc ctgtttcttc atagacttct ttacgcacat tttcttcagg
   102781 agacaatcct atttcagcaa atccaccagg aagtgaccat tctttcgtcc gtttatcttc
   102841 gattagtagg accttgccat cttttttgat ataggcacga acatctactt ttggtgttgg
   102901 atgtccttct ccaatcgttg tgagcgcttt tatttcttcg aaagtttctt ttcctatttc
   102961 actgatcagt tccaatgaaa tcgtccggat ctcctcaaaa cgctctttat caaatacatc
   103021 ttttccataa tatagaccag cttgagaaag tgcgagtagt cttttgtatt gagccaactt
   103081 cttgttcatt ctcttccctt cttttttagc aaatgtatga ctatgtaatc aagtatagaa
   103141 gactggttta attacggcaa ataaatataa tgaaatctgc aataaataaa agaatgttaa
   103201 aaaactcgag aacaaaagac attcgacttc tctggggctg tgaataaatg gcgttcaaaa
   103261 gcttgcatct gcggtaataa gccccaccaa acgaaaaata tgacgagcta ttcttgtctg
   103321 gctgttctta ttaattgagg caggacagct ttttcacaac ctccccacta aacggtgttc
   103381 tcctaccaac tcctcggtca caagtcacaa tattcatcaa acatgagaag ctgttttttt
   103441 gaaattgctc cttgtgactc ggagctgaac gacaggctca cgacctctcg gtaaacagcg
   103501 ttcaaaagcc aatttttcga tattaagcca tttctttgtt acttattgtc attgatttct
   103561 ttttctaatt tatccaatag tggagtgtaa tccacttgag tatagtctgt tacatcaaat
   103621 tcatatagtg ttcctaactg gaatttgtca tattttcggt cggcgatgat ctttttgaat
   103681 gcttctttcg taatatgatt ggtccctttc gttcgatctt ccaatacgtc accatagtga
   103741 taatgatcca tgatatagct caaatgacga tctgattcac gatcacgttt gtaccgacgt
   103801 tgacataaca cttcaaagtc tgctgttagg cgaatcgtga tcacctgata tccgtatcta
   103861 tctgctaatt cttggagtcg tgctttttgc ttatcactaa aaggatactc ggacacaatc
   103921 actttctttc ctgcttccat atacaaatca agtgctttgt aatagaaagg ataaactttc
   103981 gtcctttctt gttccgcccg ttcttccaaa ttgttaaacc cgatggattc tgcatagtat
   104041 tccttgattt catcgagcgt gatggcatac atatctggaa atcgttcccg tagtttattc
   104101 ataagatatg tttttcctgt accaggacta ccagccagaa gaatgaaata ctttttcatc
   104161 tcatttattt cctaagttaa ttggactttc caagttcata aacagtccaa tcttatgctt
   104221 gacaatttct tttactgctt catttgctgg aatcacatta tggcgaagag acttgttgat
   104281 ttctgtttca ctaaaggttt ctttagctac tttcgtccag ttgactaaaa gctccgttcc
   104341 aacattgaat ttgcggatcc cgttattgat caattcagga taatcttctt ctttcacacc
   104401 tgttcctcca tggataacta gggggacatc aactactgca tcgatttctt tcagcaaggg
   104461 aatgtttact tctgtttttg atttgaattg tccatgattc gtaccgatag caatagctaa
   104521 tgcatccacg cctgtagctt ctacaaattt cactgcgtct tccggtcttg tatatacctt
   104581 gtcattttca gcgacatgga ttccttcttc tgtaccgccg attgttccaa gttctccttc
   104641 tacggaaaca ccgtgcgcat gggcatattc tactactgct tttgtcttca ggatattttc
   104701 tttgaaaggc agatgggacc catcatacat gacagaagta tatccgctgt cgattgcttc
   104761 tttgatatca tcaaagtctc gggcgtggtc taagtgcaag actgcatcaa ccatttcttc
   104821 ttctgcaata gctgtcatta cattgactaa cacatgcatg ccgatatatt tagcggtatc
   104881 gacacttgtt tgaataatga tcggcgaacc catttctttt gctgcgccta gcatagcagg
   104941 aagcatttct aggttatgcg tattaaaagc tccaactgta aaattcaact cttctgctgt
   105001 ttttgttact tctttcaatg ttgtatacat cttttattgc caccttatct ttttatttgt
   105061 ttcctttgat cgttacttgt tttgcaattt gtcgcatctc atccattttg cccatgtagt
   105121 aatcgatccc ttgatcatca ccagcacgag cagcatctgc acgcttctgg ttatagaggc
   105181 tcatcagttt tttataccca tctccgatcg tctgtttatt ttccaaactc ttttctaaat
   105241 attggatggc ttgatcgttc acatacaatt cagaaaattt aagtccaact tgttcataaa
   105301 gtttcgctaa tttatgctta tcttgggtag tggaaatctc ttgtttcaat tgtgtaatct
   105361 ctgattgaat cttttcggtc gcttctggcg atagggcttt ttcttctttt gccagaactt
   105421 ttttcggttc tttttctttt ttcttaaaaa atccaaacat cattttccct actttcaatt
   105481 aaaatgcttt caaaactggg cttaagatcc aggcatataa gagtgctgcg gctactgtat
   105541 ctacatcggt tgctgtagca ttgatgaatc ccatcgaatt aaagatcgtt actagcaaag
   105601 cgggcaataa tgtaataaag aaaccatgag caataccgcc aatgatcgct cctcggcgtc
   105661 cgccaactgc gttgccaaat atccctgctg tccctccggc aaagaaattg gttagcatac
   105721 caggtaaaat catcgctaag ccgaacatgg gcaaagtaaa catggcaata atcgtaccaa
   105781 tcgttgtcgt aatgaaacct aaaatgacag catttgggct ataaggaaag aaaactggac
   105841 aatctaaggc agggatcgca tcaggaacta atttcatagc tatccctcga aatgccggca
   105901 cgatttcacc taacagtaga cgtacaccag ctagcaatac atacacaccc actacgaatt
   105961 ggatggcttg taaaaaggca aacatgatat agttttgatc tcctgataat tcagaagcga
   106021 atggttcgcc tgcaaataaa acagtgatga tatatagcgg gaccatcacg accatgacag
   106081 acaaataagt atcttgcaga aattcgaaat gtgtcggcaa cttgatatct tcgatcgact
   106141 ttttattttc gcctttctca ccaaaaagct tagcaacacc ggcttcaaat aagtacccga
   106201 tcgtacaaaa atgacctaaa gcaatatcat ttgatccagt gattttacga atgatcggtt
   106261 gcgctactgc cggcatagca atggcgaaac aagctccgat aaaaccaccg accaagatca
   106321 atacgatccc tcgcagaccg gcaaaatagc caaagactgt cgtcatcgtt gccatccata
   106381 aaattgcttg accagttaaa aaaatgtact tccattttgt gaaacgagcc aaaataatat
   106441 tgaatacaaa aatggctaag aacgtcagtg caatatctct acctaaaccc aaatcattca
   106501 ttgcttgacc attgatagat tcaatagaag ggatgatccc ttgcatatga aatccttcag
   106561 taaagatttt cccgaaataa gttaaacttc caacgatgat gctcgaaccg gcactcaaca
   106621 cttgaaatcc taggagtgtc tttaacgtgc cagagatgac ttgtcctgct gatttcttct
   106681 gcagtagtag acccaacata gcaatcaatg caatggtgat cgatgcctga gtcaaaatat
   106741 tttcgatgat aaaattgata acgcccatgt ttttaccctc ttttcttttt gtatttagat
   106801 aactccctta tcttgcagaa ctggggtgat tttttcttca atttcatttt ttgaaacaat
   106861 atttttcaaa tagatcatcg ccgttttagg atcaactgcg aacttttcga actgcgaacg
   106921 aaaattttct gctgtgatga tcacatctgg tttcatggaa actgcggaag aaatatcaca
   106981 atggtccagt ttggcttcta ctcctaattt gttcaaaaca tcctcagcag acatctgtgc
   107041 tgcaaaacta cttcctaaac ctgcaccgca tacgaataaa atgttcaatt tgttcatctt
   107101 tgttctcccc cgtatcattt attatttctc aactactgaa aaaagcagtt gtttgaaatc
   107161 ttcgatattt gtgcattcaa tcaatcgatc caacttctct tcgttgttga tcaattcaat
   107221 tagttctttc atgatgttca gatgcgagta ggagtctact gctgccaaac aaaaaataat
   107281 ctttactgga tccatttcag gattaccgaa atcgattggt tgctcaatcg tagctacgct
   107341 gactcctaat ttattgaccc catcttctgg tcgggcatgg gctagtgcca tgtgtttgcc
   107401 aatcacgata tacggtccgt aatcattcac tgcacgaatc atcgcttcga catagcgctc
   107461 ttcgacaatg ttttctttaa ccaatggtct agcagctagt tcaatcgatt cctcccaagt
   107521 agtggctttt tctttgatca tgatattgct gtccgtcaaa atatctttta acataggctg
   107581 gatctccctt ttgttgattt ctaaattgtt tgtttcgaat aatttttcta acttgccata
   107641 gatgattcga tctactcggc caccactttc tttcatgaca tctattattt gagtgaataa
   107701 ctccgtcgat tcatgactgt taggctctaa ccgctgtagt tcttcatttc ttttcaaaaa
   107761 gtcttctata accaagatat tcgtctcagt taaaatcgga tcgacaacta aaagcggctt
   107821 atttcgataa ttcaattgaa aggtgctgaa aaccagattg acatccaatt tatctactaa
   107881 ttcgatttct tttgaactta aaactgcagt aatctcgatc tgaggaaact tttctttcag
   107941 attctctgct aacagatttc cagtagccat cccatggtta cagacaacga ctgatttata
   108001 aacataattc aagtctcgct tgattacact ggcgatcgtc gaaaagtgga tgacaagaaa
   108061 agctatctcg tcctccagta tcttgccacc aattacttct tcgatcgtag gaacaaaatg
   108121 agtgactgcg cggtagattg ggaaataatt tgttcggatg ttttctttca atggattcat
   108181 gacttggata tgattcttga ttcttacaat cagtgcagcc atgtgcttat agagattttc
   108241 gaataaagct tcttccttta gatggaaagg tacgtgtgtc tcgttctcta caaattggat
   108301 caattggatc gttagtaatt gagcttgtac ccactcgatt gaattggaga tatctctagc
   108361 attgaacgtc tcaatagtga aacgaagata atttctttct acttgcggga cattgtccaa
   108421 ttgaaattcc tcgatcatgt ggtcaataaa tgcagaaaag ctactgtctt ttatttctgt
   108481 atttttccaa ttaatggcag agataaattc atgtttcctg attcgctgga tccagatcaa
   108541 tgtaaaaagc aggatttgtt ttttataaat atcatcttct tgtttcaaca acttattgga
   108601 atagatctcc tgtatttttt tgatttccac caaaggaaaa taatggagaa agattttttg
   108661 agccggtgtt aattgtgcat cttgattcat aaagaagtcg gtcattccca gattctgatt
   108721 gataaaatca tagatcatcg tacggataga tcgttctgaa cctttataaa ccagtccttg
   108781 ctttccataa ctgacgatct ctatcccata actgaccagt gttgctcgta accgtctcat
   108841 atcttcatct aatgtacttt tagagatttg aaattcttct tcttttttgt ttagatggat
   108901 cgggattcgg ctgtaagcaa cggctaaaac tagatcaaaa atacgttcgc gccgattcaa
   108961 aaagccgtct ttcacatctt ttatttcagt ttctaatcga gcttgctttt ccttttccag
   109021 cttcaacaaa aaaactttgt tgcgaattgt acggatctcc ggaagctttt tactgctcaa
   109081 ccagatattc gcttcaagaa cttcctgacg gatcgtccgc tgactgacat tgaactcact
   109141 tgctagatgt tttgctttta ctggatattg ggctgtcatg attctgtgaa gtatttcaat
   109201 tgttcttgtt ttcatagaaa tgatctcctt ctgatttcaa gtatatggtt ccgcttacat
   109261 aataaaaagt agatatttct gccacttgaa gtggaaagaa agtaagcaaa gcaaacaaaa
   109321 aataagccat atgctttttc atctcctcaa cgaaaaagac atacgcctat tattacaaaa
   109381 attttgccca tctatacaaa aaagaggttg tgaaaaagct gtcctgcatc aagtaataag
   109441 aacaactaaa ctaaaatagt gcttcctatt tttcgtttgg ttgttcttat tatcgcagat
   109501 gcaagctttt aaacaccatt tagtggagag gttgtgacag agatgttcag ccttgagcga
   109561 taagaagcga ttttgaaaaa tcgcttccca tattttacaa aatcgtgcct tattgccgat
   109621 aggctatctc tggcacactg tttattcaga aaaaggagct gcgacaaggc ttttgtcaca
   109681 actccgttca taaatatttc ctgtcaatcc agcataatcg gctgaataaa atcggcttgc
   109741 catctttttt ctttctcaga aaaatcaaaa taccatatct gagaaaaata ctgtcgttgt
   109801 gtcttacttc ctgagatcaa tcgcccatct tttcgatcga ctgaataatc acagcatctg
   109861 aaatcaattc ggatggcaaa actattttcg tggatctgtc gataatttcc tacttcctga
   109921 atagttattt ttttagtatg atttcttaca ccgtctgctt tgtttttttg cagaactttc
   109981 tgatgttcgg caaacagctt gtctgtataa cagcttctgg ctttttcagg agtttcttct
   110041 tcccaagcgg attggatctt catgaatatc ttttggattt ccagaaactg tgctttcttt
   110101 tctttagccg ttcctgacat gcgggacaat atctccgctt tcagcctttt cttttgtctt
   110161 ttattcttga cgatagaaaa agcagaaacc cctcctactg acaaaacagc taatgcaaga
   110221 aatcccgttt gctcataact agaaggacct gtacctggac cataataccc ataagaatct
   110281 ctgccgtaag aacgactgcc aaaataactg cccccatatg aacgaccacc aaatgaacct
   110341 ctgcccgaac ttcgaccgct agatgtacct ccaccatgac cacccgcgac agcgaatcct
   110401 tgggtcggca gagcaaacaa acataggccc actaacaaaa tgattgctaa ataattgatt
   110461 tttttcattg tcttcctcgt tatcttttat tacaaaaaac tatttttatg atagccttgc
   110521 taaaaggaag agtcaataac cagatgcata aactgttttt ttagcaattt tgtcttattt
   110581 agtcattgag taacatcgga caaatgtatg tgtcggcagt ccataattcc tctatcttca
   110641 gcatgaacaa gcttgcagac aagagatctg cgcttcctcc aggagagatt tggttttcct
   110701 gacaaaagtg caacagtgct gtaaatggac gatttggtaa agcactttga ctagcagcat
   110761 gagcaagttt ttgcatttcc actaacgttt tctgatcact gcgatgcaat acgcatgtgt
   110821 catctagcga actcatcaaa actaacaaca tatgcatttg tttctgtaca tatgaatcat
   110881 tatctagctc cttagctgct tggattgcaa gcctgatatg cggataccct aatatcgctt
   110941 cttcatatgc accatttacg ccatattttt gcttcaccct atgaccatga gtggtttttt
   111001 tatgacgata ttttgtatca ggaaaagcag caagcttgct gacatcatca agtattttcg
   111061 ataaagaaaa aggctgttcc atactgattt tttgtgcaat gacactgatc aatagcccca
   111121 ttgcccaaat cgctccttta tgggtattga tgccatttgt agcaatcagc attgcctttt
   111181 ccgcttttcg cccaatctca gcaattcttt ctcttaactc ctgatcaata gcatgatgcc
   111241 atgctatttg agccatttct tcaaaaaaag gagttagcgt cttagcagac ttaagaaata
   111301 aagcatagtc catatcggaa tgtgcacccg catccagtgc atccactaat ccaggttttg
   111361 gacttaatgt tacttctgcg atcaatgctt cctctaccaa cgcagcgagt tgctgagtca
   111421 ctgctttttt tcgttcgtca tttatcatga cactcactcc ttcttcctac tattacttgc
   111481 tgaaaccttt tttgatagct acccaaatga tatgaagtaa ccagcttaaa gcaaatgcac
   111541 aacttgccgt cattagataa gctacaagaa tcatgactcc tgaaggtaac tgcagccaac
   111601 tatctttaaa caataataac agtacttgga aaacaaatac attggctaaa tacgtacgat
   111661 aagcataaac agacaaccat ttgaacaatg gcaattttgg cgaatcgttt acaatcattt
   111721 tggaagcgcc tatgaagaca gcaaagatga tgactaaact gtataaactt tggatcgtat
   111781 tcaagtaagg tgcattcgca aagctcaagt ctcctggatg tgcgagcaat gaacggacag
   111841 aaaagaacgc gataactaag gcaacaggta agaaaagata acgtactctg tccaaatatt
   111901 gatagacttt ttcgtgataa acaaatgcgg ctaccccata aatcatgaaa ctagcaacaa
   111961 accgatctaa taagtaccag ctgtcatgat aaggcccagt caatacttgt gtttgataga
   112021 acagatacca tgcgacatat aaaatcgttg aaccaatcaa tactggccat acatattttt
   112081 tattagcaaa tactttgtaa ccgagccaaa caaaaaacgg catgaacaat tggatctgca
   112141 gcatcataac ggtataccat aaatgaggcg ctccatcacc tgtgacatat ttcagtaaaa
   112201 agcttgcagt atccgtatac ggagtttgtt gttggactgc tggaaatagc caaagataag
   112261 ccgtcgtcca tagaagatac ggaacgacta gttcaaaaaa cttttctttc aaaaattccc
   112321 aataagacaa atgctgactt gtttttacca tattataaac aattgcaaaa ataaacattg
   112381 gtgctgtgta tttaacgatg acgtaaaagg taccaagaaa agctgtattt gttgcgctgt
   112441 ggctgtttgc caaagcaaag ctcaatatcg tttgcatcat aacagccgtt acagctgata
   112501 ctttctggaa gtcacttacg ttgattttga tttttttgtt ttcaaacatc tctttctctc
   112561 tctttttctt tattaccaaa gttctttggt taatttaggt ccttcgactg ttttcaatag
   112621 aatttttttc tctggctctc tcaaatactc ttttagtgaa aaaccgcctt gccgagtcac
   112681 tacttgggta tcgaattgct caaaaaaagc aggatgcgat tgaattacct gaattggcaa
   112741 ttcaatcggt gaatttgcgt acagcagcaa atcaaaatca ctggtttctt ttactgccgg
   112801 aatcccagtc gataattcaa atcctaagct gccgccgatt ccccatttac atcctgataa
   112861 tatgtttcta gcagcatgaa attgctgata aacaggataa tccatgattt tctcttgtct
   112921 atacgattct tgttcgatga tgtcccacgg atgaatgact tgtttgacgg catgttttga
   112981 gatttctgtt gaccatctct gattgcgggc aggtccacgt aaaccaatcg gtagtatttc
   113041 tgcgtgatcc gtcgacgcac gacgaactgt tccgaacatc ttttcagtta gaacgaccca
   113101 ttcaggcaag gtgaaccctt ctatttcatg tttatcaaaa aggacaatat catgtgcttg
   113161 aatcattttt tatgcatccc attcttcgtc cattttggca cggactttat ttgtttcgac
   113221 acgtccgaat gttttggctg tttcgtttgt gtatcgattt gtcagcatcg tgtcatttgt
   113281 attgcggaca tcttgaatgg cttcattgat tgcttcgatg actttgctga cattttgttc
   113341 tgaagcagct tggtcggtga tatcttctag gaaatgatag atcgctccta gttttgtata
   113401 attattgata tcgtaagcga tagctggaac tttttcagca gctgcttcga tttcagcaac
   113461 cgaacgtttt gtcacacgtg cagcactttc tttagacatc gcttggacgg taatttcttt
   113521 attgtttaaa gcaatcaagc ggcttgattg taatccatgt gctaaaaatg cacctgaaat
   113581 agcatttcct gcaatcaatc caataactgg atgtccagct tgacggagtt ttgcataagc
   113641 agcagcacta ttagccaaag ccacatggat accgatcaat tcttcgttat agccgtatgc
   113701 ttggcttggt acatcgatca caacgatgat tggccgtttc acttttttgt ctttatcttc
   113761 ttcgtaaacg tgatccaaaa tttgtcccat gcggaaacct tcaactaaac caacttcacc
   113821 gtttcgtaca cggtacatcg ggtttttagg atcaggtacg attgatgtca cgatggcttc
   113881 ttctatgaat ttacttgatt taccataata cacagtacta atagaagaag ttggattttt
   113941 catgccagtt agtgccgcaa accaatcgta accaacactt gctgcagctg gttcatttcc
   114001 ttctgctact tctggtaagt caattgattt tgcttgatgg ttagcaattg cttcattgta
   114061 atcgtcaatg gccattggtt ttgaaagatc taaagaatca agtaatgata aataaaatgc
   114121 tgagctttca ctgcgatgcg tgtcttttcg ttcgtccatt gctgcgatga cagcagcttt
   114181 gattgcttct acactatcag tcactaattc atcaatgatt tttgattcta cccgttgacg
   114241 agatcctaat gtattccaaa tcaagtcttt gtcactagaa tcaaattctc gaacgcctgc
   114301 ttcttgttca ataacttctg gtccattcaa accgactctc gctttttttg ttgcgattaa
   114361 ataactcatt aatgctgaag taatcgacat accgccaaat gatcctacac gtcctggaat
   114421 caaaccgatg actggtacat attgttttag agcaatgatt acatttccga tttctgaaat
   114481 agataacagt ccgtagtttg cttcttgcaa acgaacacca cctgtatcta gtacaatgac
   114541 tggaaagatc tcatttcctt ttttattttc ttctaacaca tgttcaagtg cagcaacgat
   114601 ttttgcaccg gaaacttcac cgataccgcc accttggaat tttccttcca tagcgattac
   114661 tactgcttgt ttgccattga tcgtgccacg gctgacaacg atcccgtcat cactttcagg
   114721 gacgattcct tgtgctggta aacttggcgc gatgatttga tcgaatggat caatcaattc
   114781 acgtccattg tcaagtagag cgttcactcg ttctctggcg tttaattcta caaaactatt
   114841 tttcatctga aagttcctcc attgcttgcg tcaatcgtag gtacacaact cctggtgtag
   114901 caccgaagtc attgatcacg atatctgctg aaattggata acgatcaaaa aatctcgtta
   114961 atacattgtt ccatacttcg ccgaagccat cgcttcctgt acaaattttt acagtagatt
   115021 ctgttttttc tgttggaaac atcaagattt ccaaatctcc tgaaccgacc actcctacat
   115081 ggattttctt attgatcggc tgtttcgttg gatatttata agctaattgt tccatggatc
   115141 tctccccttt ttgtaaaaca gagcatacac gttctgctta ttcgtctttc ttcttccatc
   115201 tgggtaaatg gcgacaaaag gtttttctcg ccaattttat gaccaagttc tgaatttcat
   115261 tggtggttca tacaggccat ctgaccattc gatcaaatca tcgatggttt gtgcagctaa
   115321 taatgaacgt ttcgcttcac tacgtttgat gcctagatct tctggtaatt tcacgatgcc
   115381 ggctgcacgc aagctatcta gtgattcttt cgtgctagtc attccaagag gtgtaacacc
   115441 gccaattgct gcaattgctg cttgtcgttc ttcttttgtt tttgctttgt acaaataagc
   115501 aattccttct tctgtcacga tatgtgtagt atcttcgcta taaatcatca ctggtgcatt
   115561 ggctaagcct gcttgttttt gaacagctag tgcatctaat tcttcgacaa agactggttt
   115621 tttatttgaa ccatatgttt caaccatttg aacaactaat ttttggcctt tccctaatgg
   115681 atcatcattg tcgcgaagtg attgccatgc tggtgtagaa tgacgacgac cgccaggatt
   115741 atgtcccatg ttaggcgcac cgccaaaacc agataaacgt ccttttgtta cggtagaaga
   115801 gtttccttcg taatctaatt gcaaggttga accgataaac atatcgatgg catattgtcc
   115861 tgcagcttgt gataatgttc ggttactgcg catcgtgccg tctttaccga caaaaaagac
   115921 atctgggcga gcagcaatat atttttccat accaacttcg ccaccaaaac tatggatgct
   115981 ttcaacccaa cctgattcga tggctggaat catcgttgga tgtgggttca atacccagtt
   116041 cttcgcgatt ttccctttta accctagtga ttcaccataa gttggcagta acaattcgat
   116101 agctgctgta ttgaatccaa taccgtgatt caaagattgt acgccgtgtt ctgcgtagat
   116161 tcctttgatt gccatcattg ccattagaat ttgcaattct gtgatgtttt gaggatctcg
   116221 tgtgaataaa gcttctaatt gataaggttt atctgctttc acgacaacgt ctacccagtc
   116281 accagggata tctacacgtg gtaaagaatc gacaacttcg ttgacttgaa cgatcacgat
   116341 accatctttg aaagctgcag cttcgatgat tgttggtgtt tcttccgtat tgaaacctgt
   116401 gtacaagttg ccgtctttat ctgctttatc tgcagctact aaaacaacat ttggaatcaa
   116461 atcgatgaat aagcgtccat aaagttccaa ataagtatgg atatcaccta atttcattgt
   116521 gccgtcttca atcatttggg cgacacgcaa gctttgtgca ccagcataag agaaatcgat
   116581 cttgcttgcg attccttttt caaaaatatt caaatgttct ggtcgtgaga tactagacat
   116641 gatcaaatgc agatcattca ctttttctgc atctactttt tcaagacttt ctgaaagaaa
   116701 acttgcttgt ttttgattgt cgccttctaa tacaactttg tcgccgggag caatcagttt
   116761 ttccattact tctacgattt tttcagtttc tacatacttg ccattgagca agttgctgat
   116821 tttttccaat cgctgcgctt ttttttcgcg tttagaagcc cagttgcggt ttggtttttc
   116881 cataattagt catctccatt tttgatatag aaacgtggcg tcatcaatac catgttggtc
   116941 tagattgatc gtacggatcc ctttatctac accaaatttt gtatgaatca gctttgtcaa
   117001 agtattgccg gggggaaatt caatcgtcag atccattcct aattcttttg ccaagtccat
   117061 catttggttc cactgcacag ggaaagctag gttttccaac aaatctttac gaatctcttc
   117121 tgcgtcctga gtggctcttc catccgtatt ttttatatac aaacatctag catcagatag
   117181 atggacagac tgcatatacg gcttgaattt cttgaccgta tcattcatca agagacagtg
   117241 agatggcaca gggactttca gcagcttcgc tgtttgcgct tggtgccgtt tggcttctgc
   117301 caacgtaacg gataaacctt cgatactccc agataacgta tgttgcatcg gacaattttc
   117361 atttgacaga taaactggat ggtcaggatc aaatgttttt gctacaattt cctcagcttc
   117421 gcttctcgtc acacctacta ctacgcccat tgcataaccg cttggataag cttctttcat
   117481 taagactgcg cggtgataga ctagttccgc tgcattctca aatctcaaag atcgacaagc
   117541 aacagctgca caaaaagcac caatcgaatg tcctgctgca atatcaggct gaataccgtt
   117601 tttttccatt tcatctaaat aaaatactgc ttttgtcagt aatgctagct ggataaaaac
   117661 cgtatcttga tagttttctt ctttatcaac taaccgatac cccgtcacat tttctaaacg
   117721 ttccatgtac atttcaggga tatccgctaa catccctaac ttctgactgc cttgtccagg
   117781 aaataagaaa cctgtactca tgagcctcgc ctcctcagca cttacttttt ccactgctat
   117841 cttagtcaat ttctgctata ataaaaaatg ccaaaaaaag aaaaaaccat aacaaaaagg
   117901 tatggatggg atggataatt atgaaattgc ttcatctgca gtattttaaa aaagtattcg
   117961 aaacaaaaaa tgttacccaa gcagccaaag aattatttat ttctcaacct gccttgagcc
   118021 gtgcgatcaa acatttggaa aacgaattag gtgtcccttt gttttaccat agcggcagaa
   118081 acattgagac gaccgtttat gcagaagaat tttatccata tgcagtcaag acattagaca
   118141 cattagaaga aggaatcaaa ttattgtctt cggtcaatga gaagatcgtt acttccgtca
   118201 tattgtattt agaggtagcc tctgtttcta ttccgaactt ggtccgaatt tttatggaaa
   118261 agcatcctga tatccaatta acgatcatgc aacatcattt accagatgat attaccgatc
   118321 ctgtactgta catcacttcg gagatgaagc ctggcatgac caatattcct atcatgaaag
   118381 aacctgttta tgtcgccatt cctaagaaac atcctcttgc taaaaaagaa catctgcaat
   118441 tagaagatat ccaacgcacc cccatcttga tgctgtcaaa gagcaacgcc ttccgtcata
   118501 cactggatgc agcacttgaa gcgaaaagta tcgaattgac gatcagttcg acgacagatg
   118561 atcctgcgac attacgttct attgtcaagc aaggattagc tgttagcttt ttcccaaaag
   118621 tttcttggtc ttacgataaa aatgatcctt tcgtcttgcg ggaaatcaaa gatttgccct
   118681 tgacacgaac aatttattta tcttctagct tcacagaaga tcatcctttg atcaaaatga
   118741 ttgcaaaaac gatgcgggaa ttttatttag gaaaataaaa aaaaaagagc tgggacaaaa
   118801 ttcttgtcgc agctcttttt tcgaacaaac ggtgttccag aagtaatttc ttagaaaata
   118861 agccgaaatt tcccaaaaat ttgagaagca attttcgtaa attccttctt atttcttgaa
   118921 gctaaacatt tctgtcacaa cctcttctct attgtcctag acattcattg cgttgattta
   118981 aaacttccat caatcaaatc tgctagttca tctagctttt ctattttacg tagatcaata
   119041 gagatccaat gttctttatt catgtgatag gctggaaaat acccctcttt tcctcgcaaa
   119101 attccgccaa gttcaggatc gaccttcaaa tcaagaatat tgatgatcct atggtcactt
   119161 gcatcatcat agattttttc ttctggaaca gacatcagga ccccaaacca ttttttgctt
   119221 ttttgatgtc tgaatacgca ataatcagga aattttttaa aaatatattc tggagacaaa
   119281 ttgtatgttt cattgatgtg atcgattagt tcttttttcg ttagcatctg cttccatctc
   119341 tttcttattg attcgcttta ttcaaagata ccacgtaaga atagaaaaat gaatacaagt
   119401 agttgctaat caaatacttc gatatttttc ttttttatca actcttaaat gttcaatccg
   119461 cctgtgttgg tcttgctacc atctactatt aaaatgttta ttattgtttg cccacctatt
   119521 ttttgatttt agtggtatca tttttcaagt tcatgatgaa attcttatca tcagtaaatg
   119581 ctccagaaaa ataaacaatg attgaaaaat cagttgaaac ttgaccaccg tttattttct
   119641 ccgtattctt agaatagaag ggagtctgat tgatgtattc actaatgaaa aaaatcatta
   119701 ccgaaaaaga tattcgacgt catgtttctt tagtagaaca attgctgaat catacaaaga
   119761 taaccgtaaa tgaattagct gaaatcatag ggacaaccga acgaacgatt ttttctgacc
   119821 ttcaatctat tcgctctcag ctgcccgaag gctgggacat catcagcgat caagcaggaa
   119881 tcagtttgca aaaccagcaa aatttattaa caaatgacct ttgggagatt tttttcaaac
   119941 aatcagtcag tgtcgaactg ctgaaaaatc tgctttttac aaaaaaagtt gctgtccctg
   120001 attttctagc cgaccatggg ctttcttatg ggacattgaa aagacatgtg actaaaatca
   120061 atcagcgttt agcaagttac gacttgcaga tcgatttgac gaaatataca gcttgtattc
   120121 ttggaaaaga gcgtgcgatt cgtacttttt atcatcgatt gctgattcct ttcactcata
   120181 acaactattt tttcgaggat tattcgatcc atgaatccca ttattttcaa tttttaagaa
   120241 acctcagtca aactgaacta gcggttgaaa cagaagagat tttcggaaca tgttggttct
   120301 ttatcaatac gatccgtata aaagcgaatt gccgattaga ttcttctatc cataccaaca
   120361 gcacactctc ctccctgtat gactctgcat tgaaaaaatt gtatttaacg gaaggtattt
   120421 atcttaaaga cactgagctt tcttttgctt ctttctgctt tctggaaagt tggaattaca
   120481 acaataacta tggacaagaa accgctcgct gcctgcatca ttctccattt ttagaggtcc
   120541 tcgaaacgtt tgtagaggaa ctagcttcag agctgtctct tgatcaacta aaaaaaacat
   120601 ctctgactga taacttggct ctcctcatat taaaatatca tgaatcgccg atattgattg
   120661 aagcattgga ccgtcaatac catcgttttt tagaaacata tgatcagcaa tatgcacact
   120721 tgtatcaaca gaaagagaat ttactgaacg atctaaaaaa agaaatagaa atcaatgaac
   120781 caaattactt tctccaactc ttgtctctac tgatccaaca aacgattttt tctatcaaac
   120841 cgaatttgtt caatgtctat tttattttcc aaggagaacc ttcatggaag gcgttcttgc
   120901 agcaggaatt aaaagattac ttaggaaaac gtgtctgctt cttacctatc gaactaaccg
   120961 atctttccac tatttctttt gaaaaaacag atatcttgat ttccaatttc cctctagatt
   121021 ctctagatat ccctattgta tacatttctt ctattccaac gaaaaatgag ctcaatcgtt
   121081 tgactgaact cactttcaaa acttttttat agtaacggct agcgactgta ttctctagct
   121141 gttttttctt ttttgcgaaa cagtttgttc atgatccctt tttctatttc tacaactaag
   121201 aatagaagaa tcacatttgc tccaatcaat aactgttgtg tcatcgttaa acttactgta
   121261 ccgatcagct gctggccgac tggtagaaaa ataaccatcc cttgcaataa agcaaggata
   121321 cctaatgaaa gaaaaagcgc tttgttttgc aaaagacctt ggttcaatga cggcttggac
   121381 aactctctgc aattgatcat gtaaacagct tgagctaaga cgatattttg cagaagaatg
   121441 gtttgctgta agctttgccc ttcaaactgc atagccaata aataggcagg aaccatgatc
   121501 agtagggaga catagatgat ccgaaacaaa tgataaccag ataggattcc ttgctttgga
   121561 tctcttggtg ggcgcttcat gatttctgga tctggcgact cgaaccctag tgcataagat
   121621 aaagtgattg tcgttaccat attgacccat aaaatctgta ctggggacaa aggtagcggg
   121681 cgattcagca taagtgccca aatcaagatc aatccttgag ctaatgcagt tggcaagaag
   121741 aaattgatgg ttttttgcaa attatccaaa atccgccggc cctcttttac ggcttttgca
   121801 atcgtatgga aattatcatc tcctaaaacc atatcagctg cctgtttgct tacttcacta
   121861 cctttgattc ccatagcaat tcctacatcg gatcttttca aagcaggtgc atcatttacg
   121921 ccgtctccgg tcattccgac gatttctcca tttttctgca aagctttaac aattcgcagt
   121981 ttatgttctg gtgtcgttcg agcaaagaca tcgactattg gcacttgctg aatcaactct
   122041 tcatcagaca taagatccaa atcgatccct tctaaaactt tggcagtatg ttttaagcca
   122101 atttgttccg caatcgcttt tgctgtgtct ttgtgatccc ctgtaatcat tttcactgag
   122161 atccctgctt gttggcattc ctttacagct ttgactgcac tttcttttgg cggatcgatc
   122221 aatccggcga tccctgcaaa agttaattgg gttaaagtct catgcgtgag ttcctgtttc
   122281 gaatctactt ttttataagc aaatcctaaa acacgctgac cttttctcgc aaattctgca
   122341 gctgtctgac tccatgcttc ttgttctgca gaagaaagag ttgatttagc aaataatact
   122401 tctggtgcac ctttgacgta aatcaccgct cctttttcct ccgcatgcat cgttgccata
   122461 tatttgtatt cagaactgaa cggaatcttt gcttctattt ttttcaaagg tagattggct
   122521 gtttctgtgt gtcgaatcag cgcaacttct gtcggattgc cacttaaatt ttctgttttc
   122581 tgttcttctt ttgtttttaa ttcctgacag tttttcataa tggacaacac gcaggcttct
   122641 tcttttgcag ctacatccac aactgtcatt tcattttttg tcaatgttcc tgtcttatct
   122701 gaacagatca ctgtcataga acctaatgtt tctaccgaag gaagtccttt gatgatcgcc
   122761 ttttcttttg ccatttcatg tactccaacg gatagaatca tggtcaaaac agctggcaat
   122821 ccttcgggta ccattgacac caccaaagca atcgtagccg aaagaaggat atgccaatcc
   122881 ataccatatc gaaatgtcgt aaaaataact aagaataggc tcaaacaaag aatgccttga
   122941 aaaatttgtt tgtttaatcg gttgattttt ttaatcagag gcgtcgtttg ttgttcgacc
   123001 gattgcaatg cttgattgat tttaccgatt tctgtcgtgt ttcctgtttc tacaacgaca
   123061 ccgactgcag aaccagattg tacaagcgtt cctgaaaaag ccatattttt ctggtcgccg
   123121 atcgattctt cttctgtcaa aacggcatga cttttttcag atgcttctga ttctcccgtc
   123181 aaaatcgatt cttccactaa taaatcatgt gcttcgatta atcggatatc tgctggaacg
   123241 acatctccgg cacgtaagac aatgacatct ccttttacca gttctttcgc agggattttt
   123301 tcttgcatcc cgtttgatag tactatcgct tcttgtccca tcagctgctt caaaccattc
   123361 aatgattctt ccgcttttct ttcttgccag taactaacga gtccattgac gatcactacg
   123421 aataaaataa tacttccttc aatatattca ctagaagcaa atttcaaaaa agaggcaatg
   123481 atcaacacga tcatcagcag atcagtaaaa tgctttgcta tttttttcca tcgttttgtc
   123541 tgctgccttg cttttatttc attatatcct tgatcagtca ggcgtttctg tcgttcttct
   123601 tgcgacaagc cttctgttga tgtttgatag tttttcaata catcttctac cgtcaatctg
   123661 taccagttca tcctatcccc tcctgtttaa ttgatactgt tattatacgc agggaagttt
   123721 tttcaggcgt atcgattatt tcaggggggt ctgaaataat tcgttttgca aaatgtatct
   123781 gggcctaact gatatttcta ttgcttattc ttttatatac aaaatttaag aatacaaacc
   123841 agataaccaa taaaaaagga ataaagcgtg tcgtgctttc gaataacaag ctgaaagcaa
   123901 ttgaagtaaa aaacaccaac aaagcaagat ctttatatct ttgtgtagtg ttccgtttct
   123961 tttctttaat aaattgaaca tgtgcacaca agatcgaaga ccacacaaat agaaaacaac
   124021 tcgttgtcat gcctgccaaa agctgaaaaa acaatcgact atcagctaca aaaaaatgta
   124081 gacaaagacc ggcaaaaaat aacattcctg taattaaaat accgtgtaaa ggtacgcctt
   124141 tcttagaaag cgcacaaaac caatggggca aacttttttc ttttgctgtt tgatataaaa
   124201 tcctgctagt actgtagact gcactatttg ctgaggacaa agaagcaacc aaaatcatta
   124261 gattgacaat tgctgctccg ccttggcaat ggatcacttg cagcatagtc actaaaggac
   124321 tttcttttaa atctaaacaa tgccatggaa caacaaataa aatcgacaaa atcgttccta
   124381 tatagaaaaa gagaatacgc caaggcagac agttgattgc ttttttcaaa ttcttttgcg
   124441 gatcacttgt ctcgccaacg gttaagccga tcatttcaat tccagcaaaa ctgaataaaa
   124501 ccataggaaa tgagactacc attccagcaa agccaaacgg tagtaattca tcgatctgtc
   124561 caaaattttc tttgattccg atagactggg tatgaaaatc tgagacactc aatcctatac
   124621 ccaataagat aaaaaggaaa atccccaaga tcttgatgat tgagaaaaaa gattccaatt
   124681 caccaaacca cttgactgca acaaggttga ctgccattaa gatcattagt gcgctacctc
   124741 cgcttatcaa agctggtaca tgtacaaacc agaaatggat atacaatcct attgctgtta
   124801 attccgcaag tccagctcca agccagcaaa gccaataagt ccaattgaca aaaaatgccc
   124861 actttttgcc taaataatag tcaacgaaat cactgataga gttaaatgta ctatttgcta
   124921 aaaataaatc acccatggcc cgcatcaaac cataaatagc caaaccgcat atcaaataaa
   124981 cgagaataac agctggccct gctgatgcaa ttgcctttgc tgaacctaaa aataatcctg
   125041 ttccaattgc gccacctaat gcgattaact ggatatttcg attagttaga gtccgtttca
   125101 ttgccggttt ttccatcaag ttcacctttt tctttttcaa gcagcatggc gacttcctgt
   125161 tcccaagaag gtttctttat tttttcaata cagagtggta ctacaaacaa aatgacactg
   125221 atggctgtga ctgcaattag attgatccat gttccagctt tcatcgtcag tccgacagaa
   125281 aatccgattg ccgcaaataa aacaataacg acagtccaaa ctccaagatt gctctttttc
   125341 tctttttttc caattctaaa tggtcgattc aactttgcat gtgtataacg caatctcaaa
   125401 actgcgatcc ccatgatgac atagacgata cagtagataa cagtagttga atttacgagc
   125461 atcaaaaatg cttgattgac gccaggaatc agcaaataaa aaagtgcaaa gattgagata
   125521 atgacagact gagtcaaaat aacggatttg gatacgtcaa attgattcgt cttccaaaaa
   125581 ttgaattttg gtggatacgc accttttcta gcactttctg tcactgtctt tgctggtcct
   125641 gaggcccaag cagataattg gactgctaca ccaatgaaga caagtaaact aaacagattc
   125701 acaataagat gtggcaaacc aagaatttgg caataaatcg aaatagattg cgcgatatta
   125761 ttcaattcca tttgtccttt tggtacgaca tttgctacaa ctaaagcatt caaaatattg
   125821 acgaaaaata taaaaattaa cgcggcaaat actccgcgaa tatacgtttt aactggattt
   125881 tccaatcgag tgatatacac agaggacatt tcgataccag taaaaataaa catgatcggc
   125941 gcaaaataaa caaatgagga tgcagtcgta gtatctggta ttaatttgtc ccatgaaaaa
   126001 ctgcctaatg ttccggttgg tacgatccca actttgatcc acgcagcaaa ccctaataag
   126061 agcatcatcg tcaaaggcac gtacaatcct aaccaaacac cgacttttcc gccgattttt
   126121 gccatatcaa atttcaaatt caaaagagtg atgatccaat aaacgactaa aatgacaact
   126181 aatgtaaatt tgctgttcaa accaagcggc acattatcga taatattacc aaacaacgga
   126241 gcgagtgctg aagcgaccat caccatccca ggaaacattt ggacccataa cagccatgag
   126301 acaacgaacc cccatttttt tcctagagca ttcgataccc aaagttctgg accacctttt
   126361 tggggaaaca ttccagcaaa ttcaccagag atcaatgaga ttggaaaagc gtacaataat
   126421 actgcaacca gcatgtaaaa aatcatcgtc cagctcgttg ctgctacatc tggaatattt
   126481 cgtacacttg ctacaagggc gcaggtcatt ccgataaagg tcatggtcgt gatttgcttt
   126541 gatgaaccag tatctttcat ctttattcct tctttcaata gcgacatatg tttttgtttt
   126601 cttttatttt tgttcaagaa cagaaatgac aaggtttcat cgattcagta caaaacccag
   126661 tatcaccgaa acatccattg aacgatctta gtgaactggg tttgatacat gaaacgaacg
   126721 atccaactta tagaacgttt tataaaaaag aatatcttgg cgaggaatat atgaaaaaca
   126781 gggggaaata caattgtttt gatgaagcaa ataattgtct gttctccata tgtctcttgt
   126841 tattctttca cacaaaaatc agttctattg gatgatttcg tttagtttct tttccattgc
   126901 tttggcgatt ttttttgtat aaaaattgaa aattcgatca tcattcaaat aaggagtcat
   126961 gatagccgca cgtagcagag tgattttctg ttcttttttc cattcttctt tcgttagacc
   127021 cattctttca acaaaacgaa tcggagaatc accgtaatct tcttgtgtaa atgtagtatg
   127081 agaagtaata aaccgttcac cataaacatc gccatccata taagaagacc gatcgaacat
   127141 tttttcattt aattcattga tagcgttcaa atctgtacag ccttgttctt tgaataccca
   127201 attgaccata ttaaaatcag gatgattcaa tggatagact tcgattgttt ttcctttaac
   127261 agtaaaagtc agatgatcga gaaattctct gaatctttga gctgcctcga ttgaagcccc
   127321 aatcaattga ccatatccgg tcacattcaa tggcaataca cgatgtgctg tccagactgc
   127381 ggcggcggtt gctccagctt ttgatccttc caaaatataa gctcctaaca tatctggagc
   127441 ctttactgat ttttcaaaca catatggcgc gaaataagaa atgatatttc tcatattttt
   127501 atgtttgatt acgattcctc cagcagcata agggacatag cccatcttat gaggatctat
   127561 cgtgacagaa tccgcttctg aaatagctct gaacccttca tatacctctt tgtcaatcgt
   127621 tacgcagtgg tggaaaacat gatgttcttc aaagaattca gctaaggaag catacggcac
   127681 aaattctcct gcttcgttta gaaacagtga acgcgcatag ccgccatatg cggcatcaac
   127741 atgtaaatag aaatagatcc cttcatcttt taacctttct cttaattgga cgattttatc
   127801 tacactgtct acttgacctt cttctgtcgt tcctacgaca gcaaccacac ctagaattgg
   127861 gattttttgt ccagctaatt cacgaattgt tttttccaaa gcattaatat ccattcgata
   127921 atcctcttga actgggatag caaccatctg atctaagccg actccgcaaa tatctaacgc
   127981 cttcatccaa gaatagtgtt ttgtttgagg gaccagccat ttccctaatc tttggatatt
   128041 ttttccgcta cgggaagaag ctgctttgac ctcatccatt tcttcgtccg taaagctttc
   128101 ggtcatttcc aaaatttctt caacagataa attcaaaagt tcccactcag acattttttt
   128161 cactttttca ggcagcactt ccttgacagc taaaggaatc gatttgatgc aacgggcgta
   128221 ccataatcct tcaagattag caatagaacc atctgctgca atgtgtcccc atccgtctgt
   128281 catattgaat aatgaagcaa aatcctgtcc tacttctgct tccatttgcg aagttgccat
   128341 agaagattcc aatgcaacat tatttgggtt ccacagcatc gcatagttgt aagctaataa
   128401 agcaggcatc aatgtttcag cattcatttg tccccagtaa cggcctgctg aatgccatgg
   128461 gatcgatcct gcgcgtaatc gctgactgac ttcatccaag acctctatca tatggtcgcg
   128521 ggtggctaga taatctggag tgagcttatc tccttcgcta attgcaggca tatctgaagg
   128581 aatataattt tctctccatc caagatgttc atctaccatc ttgttcaaca acattttata
   128641 aactggtcca ttttcagctt tgtcgcctat aaagacggcc ttgatatcca tatctttcat
   128701 ttttcttccc tccatatttt gttggtcaat gtcttgcaga tacatgtgtt tatctgtaac
   128761 acctcttcac tatagcgcca aaaatgagaa attttttttt ataacatcaa atccacgtag
   128821 atttttacaa acaaaaataa ataaagtcgg atttttctga taattcaacg caaaaaagcg
   128881 aacaattttt caataaatca gtcttctaaa atagataacc taaaaaaagc gaacaaacaa
   128941 ataaaatgac tgtccttcat tcactaaaca tatttttatt tttttatatt gttaaaaatt
   129001 cacaatttat tttttaatag aattatctgt tttccaaatt ttataacata aaaaaccttt
   129061 tttaacggca tttatatgct ttttcagaaa ttaactgatg atttcaaaaa gaaaacatgt
   129121 cataatattt cccatattgt gcaaaaaaat acaaggcgga ggctctacta tgattgaaga
   129181 atatattgag aaaaatattt taagacagct gtttttatgt gggcaattct atgtaaataa
   129241 agaagtcaat ctagaaaagc tctccaatct cctgcatgtt tgcaaaacca ctttactcaa
   129301 tgatatcaat aacataaaaa aagaatttga agaacagatc gcttatacac atcgggaaaa
   129361 ggaccgttac actttatatt tttcagaaca tatacctcga tgcaagatca tgcagcaatt
   129421 gtctcaaaat tcgttgtttt taaaaacttg tctgctttat ttggaagaag atgaacctga
   129481 ttatcttcaa ttaactgaat gtgaattcat ctctgtcagc aaagcttatt cattaaaaaa
   129541 acaagtatta gcttacttta atgactgcgg gatcgaaatc gaccgctact cccctcgatt
   129601 cactgaaatg gaaagacgtc tgctgcttct taatgtgtcg tatcgtttag gcggttttaa
   129661 ttcttgggag ctacctgaat cgttttttga acgtgccgat cgtttcattg aatccgtaac
   129721 ggaaaatagt ggcagatttt atgataaaga aaataaagaa atcttatcga ttggttttgc
   129781 tatcagtttc ctacgccagc aagtgtgtgc tgtgacaata gattcaaaat ttatcgaaga
   129841 aatcaaaaaa cgtcctattt acaattatgt cgaatcagca tgggaaaaca ctgatttcca
   129901 aacctattat aaaaaagaag aattcgcctt tattttgaca ctattcaatc tttgtaatta
   129961 tggtttccat tcttatcagt tgattgcgga agactttcaa cagctacacc aagtgtttat
   130021 tgacaatact cccgaaataa aagaactagt tgctaccttt gaatctcact tcaatcaaga
   130081 attattcgga aaccaaccgt tcgaacgtgc gctgattcat ctgatgcgtt cggcttggga
   130141 taactatcaa ttattcatgc cagaaaaatt ttatctttta aatgaggaac agacaaattt
   130201 atataaagaa gtccagacaa ttttttccag ctggtccagc cagttacctt atgatctacg
   130261 gttgaatcct aattgcatgc gggcttttgt gatcgaattg agcggaattc tccgtctgac
   130321 aaaagaacat ctgactatct atatcgtcac aaattcggat gttcattatc tgatttatcg
   130381 ggaagccttg gaagctgtaa ctactttcga ttttcaagtt gcaccaacaa tttattcaag
   130441 tatatctgat ataaagaagt atgcgcagca gtcctcaaac cgggtattat gtgaacgtac
   130501 attatataca ccagatgcgg tccaatatga aaatattatt cctatttcga tcaatacgat
   130561 cgatcgagct atcatttcag ctgtgcaaaa caaataaggg gctgtgacaa aaatcatgtc
   130621 acagcctctt atttgttgaa gatgcccatt tctgtaacaa tctcttgata cacgtcaggc
   130681 tcacaacctc acttattttg gacatctagg ggtccgatct ctttatttaa aaaataagaa
   130741 atgatggttc ggataactac caacgccgct agttggaaaa tatcctgcaa ggtaggtttg
   130801 ataatcgatt cgatgatccc agctgaaaca agaatttcca aacttagaag tatatagcct
   130861 cccaacatct ttttaatagc attgttctcg cttactcggt cgctttgcac ttttgtatgg
   130921 acaaataaat atttcaaaaa atccttgatt gatagacaaa caccccaaac caataaacag
   130981 atagcaaaga tatcaagtcc cagtacaagt ttttcgaaat atggaagaag aaactgcatc
   131041 aactctgtcg tcatcctacc tctcctcttc tttgtttttc tttgtcttct atttcctgat
   131101 tcaagaaata agaaatgact gttcgaatga taataataag tgcaagcttc aaaatatctg
   131161 tccaagtcgg cttgataact gactcgatga tatcagcaac gactagtact tccaagctta
   131221 acaaaatata acttgctagc agttttcttg tatctgcatt acgagtatta cgttctgcat
   131281 agtttttctt gacatgagac caatcaaaaa gtttgaatag aacaagaacg atcccgacaa
   131341 ttaagatacc gatagaacaa atatttaaaa tagcaatgat ccattctaga aattcaatta
   131401 aatttgagcg aaacatatac gctacctact ttcttttaat ttgttcctat tcttttattg
   131461 taaaagaaag ttaccttgtt cgtctattta aaagttgttc taatgataac ggttcgctta
   131521 aatttttctt tttcactctc ccatttttct ccctccatcc ataaatcgtg gaagtatcca
   131581 aaacaacgta gcaagttaaa ataaggctta gtccgtcatc agcttttcaa agaagcactg
   131641 tctcggtgct ttttttgtac ccaaatttct aaaaatagaa ccatacacct cctcaaatct
   131701 tttcttctta cctaccctat ttttatttac aggaaaatca gacacttctt cttttttgtc
   131761 aaaatttcct ttttcttctc gtcctatcat ttggcattct aataaaaacg aactatcctt
   131821 ggatatagaa agagaaaaca aggaggcgtt tttcatgagt aaaaaattaa tcaaagtcgg
   131881 catcggttta ggtttgcttg cccttggcgc tgcatatcta ggaaaaaaga caggactatt
   131941 cgaagatgac agtcatttat atgatgaatt cgaatcaatc tagcatccta tttagttgaa
   132001 tcactacgga catacttgga agacattgga ggaatcacaa tgagaaaaag taaagcaata
   132061 atgattggtg ccggtattgc caatatggca gcggctgttt atttgattca agaaggcaaa
   132121 tggaaagggg atcagatcac cttttattca ttggatgatc acggttcaaa tgatggtgca
   132181 ccgaccgaca ctgtagtgga tgaatattgg aataaaaacc atccattaga aaaccaaaaa
   132241 ggctatattg ctcgaggcgg tcgcatgctt aattaccgta cctatgtaga tttgatggat
   132301 ctgttaagtc gaataccttc tgttactgag ccagggatga ctgctgaaga agatacgaga
   132361 gattttgatg caaaacaccg aacctttgac aaagcacgtt tgatggaagg tggcaaagga
   132421 atcattgatg gcggaaaact gggattcaat aataaagacc gtattcttct gactaaactg
   132481 attgctatgc ctgattcaga agaagaaaaa ctagacaata tcactatcga agaatatttc
   132541 aaagatgatc cacacttttt tgaaacaaac ttctggttta tgtgggaaac aacatttgct
   132601 ttcagaacac gtagttctgc tcaagaattg cgtcgctata tgcatcaaat gatttatgaa
   132661 ttcactcaaa tcgaacatct agtgggcgtc aatcgaacac gttacaatca atacgaaagt
   132721 attatgcttc ctttgatcga gtacttgaaa gaacaaggat gcaaaatcat tttgaaccgc
   132781 cgtgtcactg actgggaatt caaagaaaca ccgatgcaag atgaaatcac tgtgactggt
   132841 ttgaaaatta ccaatacact gactcaagaa gaagaatacg ttgtggtcga tgaagataca
   132901 gcagttattt tcactaatgg gtccattaca gattcagcta ctttaggaga ttacgaaaca
   132961 ccagctgtag aaaatatgga ttatggtgca gcttctagtc tctggaaaaa agccagtgaa
   133021 cgtttctaca acttgggaaa tcctgacaaa ttctttgctg atcgagatgc aagcgaatgg
   133081 gtcagcttta ccctgacgac gaaaaaccat ttgttgttga atgaaatcac ccgtatcacg
   133141 actcaagaac caggaaatgc cttgaattca tttatttcaa ccacaccgat cacgccgtta
   133201 ggccaaaaag atgtcaatat gtctattgtc gtgcatcatc aaccgcattt tacgtctcag
   133261 aaaccaaatg aaacagttat ttggggttat ttcctttatc caagacgacg tggcgaattt
   133321 gtggataaac agtatattaa aatgaatgga aaagaaatgc tagaagaact gattggacaa
   133381 ctttcaaaag tagatcctgg accagtgaat attcgtgaaa aagaaacaga aattttcgac
   133441 agcgtaataa ataatatccc tgtctatatg ccttatgctt ccgcgttgtt caataatcgt
   133501 gccaaaagcg atcgaccaaa agtcattcct aaacattcga ccaatctggc ctttacagga
   133561 gaatttgtcg agcagcctta tcagatgatc tttactgaac aaagtgctgt acgttctgga
   133621 gaaatcgctg ctttccattt tgcgggtatt cctatgtccc gtcttgtcaa aacaccgcgt
   133681 tacgacaaag atattcctac tctagctcgt gccgcaaaaa aaatgtttga ataatcaaga
   133741 ggttgtgaaa aagctgccct gcatcaagta ataagaacaa ccaggctaaa acagcttctt
   133801 atgtttttga tttggttgga cttattaccg cagatgcaag cttttgaaca ccgtttacca
   133861 agaggttatg aaaaagctgc cctgcatcaa gtaataagag cagccaagct aaaacagctt
   133921 cttatgtttt tgacttggtt agacttatta ccgcagatgc aagcttttga acaccgttta
   133981 ccaagaggtt atgaaaaagg cgtttagttt cgagtatgaa gaaaaatttt gtgaaaatgg
   134041 ttcctcacgt ttttgcataa atttgactta ataccgagaa actgatccct tctgtcataa
   134101 cctcttttta tttatgtctg tttctttttg aaaacaacta ttttttcaac gtttactctc
   134161 ttgccaatcc aacatcatct aacggataca actatttcaa tgattaccat tacactaaaa
   134221 aatcggaaac acacctctcc gaacacaaat acataagact atagaatgtt tcatttaaga
   134281 aaaaaagcgt ttcattatca aaaaggtaag atctccttta aaatgaaatt atctgtagta
   134341 ctaataatgc agacaatttt atgaatctga aaggagcatc tccaatgaaa gcagtcactt
   134401 ggcaaggaaa acagaaaatg gaaatcagaa acgtagatga tccaaaaata ttagaaccaa
   134461 cagatgcaat catccgtatc acagcaacag ccatttgcgg ctccgatctg catctttacc
   134521 atcatggtga agcagttttg gaaaaagggt atgttgtagg acacgaacca atgggaatcg
   134581 ttgaagaagt gggatctcaa gttcaaacat tgaaaaaagg gcaacgtgtg gttatccctt
   134641 ttaatattag ttgtggtcat tgtcatttct gcctgaatca catggaaagc caatgtgatg
   134701 aatcaaatcc agatcagtta tatggcgggc ttttcggatt tggcaagttg aacggtaatc
   134761 attggggtgg tcaagcggaa tatctaagag ttccttatgc agactctact tcatttgttg
   134821 taccagataa tgatcttcct gatgaaaaag ttttatttct atcagatatt ttgcccacag
   134881 cttattggag cgttgaaaac gcaggagtaa aaaaaggcga tacagttgtg attctaggtt
   134941 ctggtccagt cggtctattc gctcaaaaat tcgctgttat ggctggagca gagcgcgtaa
   135001 tcgcagtaga tccagtacag caccgtctag ataaagcggc taattacaat ggggttgaaa
   135061 cttatttctt agaagacacg gcacaagctg gaacagaatt gtatgaactg gctaaaggtg
   135121 gcgcagatgt cattatcgat tgtgtaggaa tggacggatt agagcctgtc aaagaaaaag
   135181 cgaaaaattt agtaagctta caaagcggca caatctctcc attacagatg gcatcgcaag
   135241 ctgtcaaaaa atttggaaca gtccaagtaa ctggtgttta tatgactcca gcttcttctt
   135301 acccgctgca agaatttttt atgcgggata tcgctgtcaa gcatggacag gcaccaatta
   135361 tccatctaat gccaaaaatt tacgcaatga ttgcagaagg actattcgat cctacacaaa
   135421 tcattactca ttcgctgcca ctatcagaag cagcacaagc atatgagatc tttgacaaaa
   135481 aagaagataa aaatataaaa gttgttttga aaccttgaat tcagtaaaaa atacaggtga
   135541 ttttctttat gcccaaaatc aaacgaaata cccatgagat cgaaatagga aaagaagaag
   135601 atatcgaaat acgaggacgt cgcttccgct tctatttcca aaatcgttac acattgatct
   135661 cactggctgt cgatttatta accgggattt tctatatttt tggaagcctt gcttccttaa
   135721 ctaaaatccc cgatcgatat ggcacctatt tttatttggc tggtgctatt ttcttgacga
   135781 ttcgtcctat tctcagaatc gtaaggaaca tttttattta cgacgaaaaa aaagcaatga
   135841 agagaacaag caaaaaaaaa gaggcatgag aatttttcaa atctcatgcc tcttttttca
   135901 ccctatcgta tcactttgat acagacgagc ggtcgtccca ctttcttcct tgaccattgt
   135961 caaaaatgtc cactcgtatc gttcataaaa cgtggtatga tctgtaatta agtaaagttt
   136021 ttcgtaccca agttgatgca tttcttttct gatttccgct aatatccgct tggctatccc
   136081 ctgcttacgc ttttccggtt caacaaacag ggcacaaaga tttggtgtca gatcagggcg
   136141 gttatggaag tcgttttcaa tgacgcccgc tcctccgacg atggtttctt tttcatctaa
   136201 gaccacatac cattgaggaa tactttcctt attagcgatt ccagtacgaa tactttcctc
   136261 atacacaaca gcaggaactc cccattttga tgaaaaccaa ttcgcgcttt gttttactaa
   136321 ttctgggtgt tctcttaatt tgatccagtt atatgtaacc aatgtttaaa cctccgacta
   136381 aaataaaacg tttacaaaaa ttatacaagt ctttatgaaa attagcaatg acaacaatag
   136441 tgatgtaaaa aaaaaaacgg aaaacaatca cttgttctcc gttttttgat taatcaagca
   136501 tctaaagctt cttttcttct ttcttggatc gtagctacct tgacgaacgg tagatagatc
   136561 aaaatagaaa gaaccaaatt gacagcggct agtacaccac ctgcaatact ttttgttgcc
   136621 aatacaccgc caataattgg tggagttacc catggaggca taacggtagc tgctggcact
   136681 aaacctgtag aagtcgcaaa gtaagcaaca ctaactaaga ccattggtgt caaaataaat
   136741 ggaatgaaca tgatcgggtt caacacgata ggtaacccaa acatcgttgg ttcattaata
   136801 ttgaagacac ctggcgcaat cgacaagttt gttacaacca tatatggttt gtttttacgt
   136861 cctactaaat aaatagctaa cagtaaacct aaagttgcac ctgttcctcc taagttgaca
   136921 aagctgtcaa agaaaggttt gttcacgata aacggtgcgc tatgtccagc tgcgattgca
   136981 ttaacgtttg cttcaatggc tggcgcattg attgtttgca tcaaaggatc gaccatattc
   137041 gctccgtgta aaccaaagaa ccataagaat ggtgtgataa atgcaagcag taaggcagat
   137101 ggataagagt ttgcaagtcc cataaatggt tcttggacca ctgtatagaa agaacctacg
   137161 atatcatcaa caccgaatgc agcaaaaatt gcagcaacta aagcaaacgc agagatagtg
   137221 atcattgctg gtagtaatga agcaaatgct tttgcaactg ccggtggcac accatcaggc
   137281 attttgatga tcaatttttc atttccgctt aaacgtacaa agatttctgt tgaaataatc
   137341 ccaacaataa tagcaataaa caaaccgttt gagctcatac ctaatgctcc gcctaaagcg
   137401 aagaaagagc ctaaagaaat cacacctgcg gaaacaccgt cttttcggta gccgttagct
   137461 aaatgataag caagcaagaa agcaatcaag acagaaagaa tggcaaatgt accgttccaa
   137521 gcagcagccc cgaatccttt ccatgattca ccaccaaaaa ttgagttcat caattcttgg
   137581 aacccgggaa tcggtaagtt attgatcatt actgctaatg ctcctaagat cattaacggc
   137641 atactcacca taaatgagtc ccgaatcgca actaaatgcc gttgtgcacc aatttttgat
   137701 gctacaggta taaaatgttt ctccataaat gcaacaaatt tatccataaa tcttccctct
   137761 tttctttact gtagattacg cgttcaatcg acgatacaga ttaatgatct cttttgctaa
   137821 atctgtaaaa gctatagatg tcatcagatg atcttgactg tggaccgtca aaagcgtgac
   137881 ttcaacgtga tttccttgtg cttcttgtgt cagcatctct gtttgagagt gatgtgcttg
   137941 gactaaagat tcctctgcat ctttgatttt ttcatctgct aaagtgaaat cacctttttt
   138001 cgcagcagca atcgcttcca ttgcatcgct ttttgcattt ccgccaaaca tgatcagacc
   138061 catgatggct tctagatttt tttgatcttc catttattgt atcctccgat aatttttatt
   138121 atttgtttat tagtttttct gcttggtcga gtactttttc accattcatc atgccatagt
   138181 cagccatatt gattacatct aaaggaatgc ctttaggtgc tagcttttca gcaaattgat
   138241 tttgcataaa gcgtacttgc ggtcctaaaa gtaaaacgtc tacattttta gattctagtt
   138301 gattatccgc atctgaagca gatacagcaa agatgtctgc ttccattcct tttgcttctg
   138361 ctgccttttg catttttgtg actaggagac ttgtactcat tccagctgaa catacaagca
   138421 tgattgtttt cttttccatt ttaaacacgt tcctttctat acttacactt gtatataagc
   138481 aagtagcgtg ccaactttaa aagcgcttta aaaagttgat atcatcacat ttaaggtcta
   138541 taccaagttg catacacact aaaaaagaaa gcgcacacta aacacacaaa ttaattttta
   138601 ttaccttttt tcttttaacg tgcacaaaaa aagttgtgac acaaaacttg tcacaacttt
   138661 tttcaaataa acggtattca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
   138721 acataagatg ttgtttttac tttgctattc ttattacttg atgcaggaca gctttttcac
   138781 aacctctcaa taaacggtgt tcaatcagct gaccctgggt attagctcaa aacgctggat
   138841 tcacaacctc ttttatttca aataaccagt aaatacaatg aacatctaca aaaaacttct
   138901 atttatttat agatttcgtc aggcaaatcc aattctctct tatcaatcaa agtctgatac
   138961 cacgaagcag attttttcaa tgttctgctt cgttcatcgt tcaattcgat tcgaactaat
   139021 ccatatcgat tcttaaatgc attcattggt gacacacagt cagtgaatgc ccataacata
   139081 tagccttcgc aatttgcgcc ttcttctgtt gctttgatca agtaatgcaa atgctgtccg
   139141 ataaattcga tacggtaatc atcttgcacg attcctttct catccatata ctgttcttca
   139201 tattctcttc ccataccgtt ttctgttatc agccaaggga tattatgata attttctttt
   139261 agatacatgg ctatatcata catgattttc ggatagattt cccatcccct ggaattattc
   139321 attttcttcc caggtaaatc aaagacttca taatatcgtt caggatggaa aggtgtctct
   139381 tcattccatt gataacgagg ggcgcgaacg cgtttaggat aatattgatt gattccaagg
   139441 aaatctactg tatgatgctg aatcgtttct aaatcctctg ttgcaggatc gaaataaatc
   139501 gattgttttt gacacaactc caggaactct tctgtgtagt atccttttac catcggatca
   139561 aaaaatactc gattataaaa caaatcatac atataagcag ctttttggtc ttctggagaa
   139621 ttggatcgag catagaccat ttcaggattt aaaatacagc caatccttcc accataattt
   139681 ccgctgcggt aaatctctac tgctttagcc gttgctaaaa ctttatgata attccacatc
   139741 atccactttt ttgtgttttg ttcatgcggc cagcgaacag catccaaata gcaacgcgtt
   139801 tggggaacga ttggttcgtt gaatgtaaac cagtttttta ccctatcacc gtaacgatca
   139861 aaggcaattt gagcatactt agcataaaaa tcaactactt ttcttgaaga ccagccatca
   139921 tatttttcca aaagaaaagc tgggacttca taatgttcca aacaaatcat cggttctacg
   139981 tctgcttcga tcaaagagtc gatcatttca cttatatgct gagcatattc ttcatctact
   140041 atacagttct cataatcagt aaaaaaacga gcccaattga tagaagtccg ataatgtgtc
   140101 agatggatgg atttcatcag atccacatcc tgctggtatt gttccataaa attagttgct
   140161 acggctgggc catacccatt atgccaaaca aaatgttcat ttttatacca gctatctata
   140221 aacgaatcct gtccttcttt ttttcctttc caaccttccg tttgccaagc agaagatgca
   140281 gcaccaagaa taaaattttt tggaatagta attttcatga tttcctcctg ttttttagat
   140341 cccatcattt tccacacgat ttgctacttt tacaaatggc agatagatca aaatagaaac
   140401 aataatacaa acaagttgcg taataactgc accgatattt cctgctgttg ccagataagc
   140461 attgacgatc ggcggcattg gccaagggac cattacaact gctttcgccg caaatcctaa
   140521 agcggttgca aaataaccaa tcgttccagt gataagaggc gttaagataa acggaatagc
   140581 taaaattgga ttcagcatga tcggcatacc aaaaataact ggttcattga tgttgaagat
   140641 cccaggagca agggataact tggtgatttg tttcatatct tcccgttttc caactaacaa
   140701 tactgcaatc agtaaaccga tcgtgactcc agacccgccc atactcatat acatatccca
   140761 aaatggcata gtaataatgt tcggtacttc ttttccagct tcaaaagctt ccgtatttac
   140821 agcaattgcc gctaaaagca acggttcccg tatcggcttc accatttgat ttccatggat
   140881 accaatcacc caaaagactt gcgcaataaa catcaatagc aaaatacccg gtaacccttg
   140941 tacgacacct tctaatgggc gttgtacgac attgtaaatg atatcataaa ggtacattcc
   141001 tgtaagttct ttgatgataa atcccgcagc cgcgataccc gatacagtca gaatcgtcgg
   141061 gaataaagca gaaaaactag cagaaacatt cgttggcacg gtatcaggca ttctcagctg
   141121 tagtttcttc tgtttcctca accagcaata aagttcaata gaaaggatgg agaccaacat
   141181 tcccaaaaat aaccctttcg tatctgtata ttgtttagcc aatacgtttt ctactataac
   141241 agtctgattt tcattcacta attcataaac agttggatta acagataaat aagacatcac
   141301 tgcgatgata ccagggaata caccatccac tttattcagc ttggctaatt cgatccctat
   141361 cagaaaaaca gcatagatgg tcaagaaact tagtgtgaca tagctgatgg cagatgcgat
   141421 aggctttaat tcttctaaaa aacgcaagaa acttatctgt gctaagccat tttccgagtc
   141481 aaagaccatt gctgaaaaca atgtgccaaa agcacccgta ataattaccg gaatcaatgc
   141541 agcaaatgcg ttttttatca ccatgatata ccttaaatta ttgattctcg ttgccacgtt
   141601 ccctaaaaca ttggctaatt tatccataaa actcattcca gatcccctca ttcctgcttc
   141661 ttctccttga cgtcatctct ttttgtctat tagtcagcgc ttacatttcg atgaacagaa
   141721 actatttact cctctttttt accaaatata aaacaaaaat tcatctgatg tatttatcat
   141781 aaaatacatc agatgaactg tcaattagtt tctttttctc ctattatttt ctgtaattct
   141841 ctccgattta ttgccatatg gacagtcatt gcatcaaacg cagcaagtgc attccgcctt
   141901 tcgatggcct gaacgatctc tgtatgaata tcgatcgtct cgattttcag atgctggata
   141961 tcataatttt tattaaacaa agcaattgat tcattaatga ttgggataat atgcagcaag
   142021 gctacgtttc cgctagcttc tgcaatcatc gtatgaaaat cgatgtctaa ttgaacatgt
   142081 tcttgtgtgc cttctcgaaa agaagtttca atcttttgcg ttaatttttt catttggacg
   142141 atttgattct tcgttgcata ttccgctgcc aaacctgcca tcttaggctc caataaatat
   142201 ctcagttcaa ataagtctcg tatcatccga tcctgatctt caatcaaaga aaaaccgagt
   142261 gggtcattgg caatccctcg cttatcactg acaaatgtcc ccgctccttg ccgaacttca
   142321 aggatattcc tggaagctag agcccgcaca gcttctcgca ccgtgcttcg ccccacctct
   142381 aaataatcgg acaattcata ttcattcggc agtttatctc ctacttgcaa ctgcatttcc
   142441 tttaaatatc taaggatctt gtcagtagtt acttctacta gggttttatt ttcttccaaa
   142501 ccacatacct ccttttcatc tgatgtattt tttcgtaatt attataacac gtttgaatca
   142561 tctgatgatt ttttgtatac aaaattgatt aatccgttca tagacataca aacgaagaag
   142621 atatttacca taaaaaagac taggataaaa ttcactccta gccttcgatc atttttattt
   142681 tgaacgacaa aaaatatgat gttcaataac agacgatctt cattcgattc ttgatgtcat
   142741 cttttgtttt ttttaatata tggataaaaa gtttgattac atcgtttcta ttctagaatt
   142801 tggtcgattc tcaattcttc ttctggtgag aaatctaaat tatccatcat tcgaacactt
   142861 tcttgtaact gacttaaccg acttgctccg accagtacac ttgtaacaga tttttgtcgc
   142921 aaattccatg caagggccat ttgcgctaat gattgtcctc gtgatacggc gattgtttgc
   142981 agtgctttga ttttttccag agttgatcca acttgttctt ctgaaaggaa tggtatttcc
   143041 ttacgatgtg ctctagaatc ttcaggaata ccatgtagat aacgatttgt cagcaaccct
   143101 tgagcaagcg gactgaacgt gattgcccct agcccttctt cttctaatac tggagataga
   143161 tcgtcttcta gtagtgcacg agaaaacata ttgtagcgca tctgatgaat aatgaacggt
   143221 gcttcctttc tttttaaaat ttcagtcatt ttttttgtat cttctccatt atagttggat
   143281 ataccaatat ataatgcttt tccttgtctt acaagttgca tcaaagcttc tgctgtttct
   143341 tccaaaggtg tatcagggtc tgggcgatga tgatagaaga tgtcaacata atctagcccc
   143401 attctttgga gactttggtc acaacttgaa atgatggatt tacgagagcc ccattcccca
   143461 tatggccctt cccacatatg ataacctgct ttgctagaaa taatcagttc atcacggtag
   143521 gcttgaaaat cttctctaaa aatacgccca aagtttttct ctgcagctcc agcagggcct
   143581 ccataattat tcgcaaggtc gaaatgggta attcccatgt cgaatgcgcc tcttaaaatc
   143641 tctctttgat tatggatagg atcataatca ccaaaatttt gccataatcc gagagataaa
   143701 atcggtaatt tcaaacctga atgaccggat ttacgatatt ccattttttc ataacgtgca
   143761 ctatctgcaa tatacaattt ttctcctcct catcaaatct ttttaaaacg ctttcttttt
   143821 tctattttta gtttactaca ttttaaagaa aaggagttaa tcaaaatgat tttttcgtat
   143881 atttttttgc catttgtttt ttactcgttg atagatcaga aattgattac gtgcttgctg
   143941 gattttttta taacgtttcg gttgtttttt gtaaaattgc tgatgatagt tttctgctgg
   144001 ccaaaacgta ccggctggtt cgatttttgt gacgattggt tggtggaaag ttcctgaact
   144061 gtctagtttc tgttttgatt gttccgctaa ttctttttgt cgttcgactt gataaaaaat
   144121 cactggacga tattgcttcc ctctatcttg aaattgtcca aatgtatctg tgggatcagt
   144181 aatttgccaa tagattgtca gcaactcgct ataacttatt tctcttgtgt cataaatgat
   144241 ctccacagct tccacatgac ctgtgtaacc accacttact tgatcataat ttggtttttc
   144301 aacatgtccg cccgtgtatc ctgacaaaac agataggatc ccttttttcg attcaaacgg
   144361 ctcgaccata caccagaaac atccccctgc aaatactgca cgttcttgat agtcagcatc
   144421 ttgcatggca tgcttggaaa agtcatattt ttgctcctta tcaaatcctt ccgtaatcaa
   144481 taaataaaag tccattacat caggcgtcag attcattcgg atagctaatg gacgtagtgt
   144541 tgcttctagt tttgataact gttctttaac ggatatattt tctcttatgt cattctttgt
   144601 ctgaatcaat acttttcgtt cccaatctcg tgtattcgga ttcaaaatca gattataaag
   144661 tgtttgaatg aatatttgtt cctcatccat agattctcct cctttccgaa gaagacaagc
   144721 tagtgacttt agctgtaatt tatcataaag tgaggaaata agcgattact tcatccccct
   144781 acagattttt cgtataaaaa agagagaatc cttatcgaca gattctcctt tttaatcaat
   144841 ttattttata aatgaaaagt taatttacct tttatggaaa actattttaa aaaataaaca
   144901 taaatatgca gttaatgtaa caagataaga aataatagtc gttactgtat aacttgtgct
   144961 tactttaata tcagcaactc ctttgactcc gcttatttgt accaatccat tagcattcac
   145021 ctgctccttt acaggttgat gatttatttc tacattcgtt cctaaataag catatacagg
   145081 aagtgtgatt acttggctct tctcattagt tactctatat atgatagaac taccagttgt
   145141 tttataagga aggtttaccc attgactgcc aatctttgct tcgtgtttta ttatatgctc
   145201 cttattctcc aatgaagcga taggagaata atcatataaa aaataatcca ttcctttagt
   145261 ggtatacgcc tctactgttt ctttgtttac aacaatatgt cccggctgag aaattatttt
   145321 ttgattgaaa ttttttttac tagaaacagt taaagtgatt aataacaaaa cagtcataaa
   145381 taaataaaat agttctcttg tcttattgta tcttctattt ttgaaaaata tcatcgttaa
   145441 tatcagagaa gagaatatta tttgcaagct taagatccgc caaggaaatt gtaacaattt
   145501 ggctggcgta ttttgcagaa gataccatgg caccaaacta gttgtagcta gccaagtcaa
   145561 acacgtacca taataaatct ttttttctat gtttgttaat tttttaaata aaatcagggg
   145621 aatacataaa gataacagac caactagtcc cattgagtaa gtagttaagt cgttgttcat
   145681 agcttcagtc agactatcga acacattaga tgcatgagca tataaatttg tcacgctagg
   145741 tttgttgatt ttttggtaga gcatttgttc aaacatcgga taccaaaaag cactcgtcag
   145801 aaccaatgtc attcctgccg cactaaataa ttttttaaat atagcaataa aatctgcatt
   145861 cttgaaacct ttagaaacca aacgaataaa aataaaaatg ataattaatg ctgtactcat
   145921 taatgttgat agtacatggc tgtacactag caaactcata ctcaatgcta gctttacaga
   145981 cggatacttt tcttcttttc cagagatgat ttgataagca taaaataata cagggataac
   146041 aagtgttatt gcgatagctt cggcaattgc ggaacgatag tagatgtcta ctgtccgata
   146101 agcagagaaa atatagagac aactaaataa aaatgccgct tttttttgtt ttaaaaaccg
   146161 ctcgccataa tgataagcta cgagtgttgt acacaagttc aataaccaaa cgtaaataat
   146221 atagcttaca atcaaatttt ttgtcatcca atataacatt acagctggaa aaaaagtcag
   146281 aaagggataa aaataattta caccatatcc agtataattg aatgtctcat aattaatcgg
   146341 actggtaaaa atagtcgata accccttgat tcgagataag tgaaaagaaa gatctccagt
   146401 ggtataaata ctctgtgtat ttttgaaaaa gatgaaagaa tatatactac taactataat
   146461 taatccactc aatattacta cttttttatt tattttcatt cttcttgttt atctcctttt
   146521 tagtcacata tcgagaatct ttcaacttat taggtgcaaa tcgtttaaaa cttgaataca
   146581 ccactaaaca ataagtcaga atactgatcc ataaagccat gagagcagtt ggagtatatt
   146641 ttgttgtaat ttctatctta ttttctccag catttacatc cacattcact aagccatgtt
   146701 ttttataaga gtcatttacg actttaccgt ttacttttac ctgtgtacct aaataagcat
   146761 atactggtac tgtcactttt tgctttttgt tgcttgccac tgtgtaagta atgttattat
   146821 ctgcagcttt atacttccct ttttcccacg attctcctac ttttacttca tgtttttcta
   146881 agtgaggcct ttcttttatc gcttctgttg gggcgtaatc gtataatcct cccatatctg
   146941 atgttgtata acgtgggagt gtttcttcat tgataacaat atgaccgtgt tttgattgga
   147001 taacttttgc gtaattttct tttgctgcta tagacgtgac gagtagaaac accacagtag
   147061 cgccaatgat taaccatgaa tattttttat tcatagtctt tttcataaaa acaagcgtta
   147121 tgatcattga agaaaaaaag atttggatac ctaagatacg ccaaggaaac tggagtaagc
   147181 ttatcggtgt tttttgaaac aaaaaccatg gaaataaatt agttacgcat acccatgtag
   147241 aaactacgcc aacatagatg attttttctt ttttctccat ttctttcagt atgaataaag
   147301 gaaaaatcaa cgcaaccaat cctaataacc ccatagtgta tgttgtaata tcgttattta
   147361 acgcttgaat gatactatct gaaatagaaa gtgcttctag ctgcaaatct gttttgaagg
   147421 gactgtgtat ttcttgataa ttcatctgct gcaacatagg ataccaaaaa tagctagtta
   147481 atgcacaagt caaaaaaaca gcctttacta aattcacgac cacaaatttt atatcgtttt
   147541 ttatttgctt gcttaaaaac aaacgaacta taatcataca agcaatcaat gcactacaca
   147601 atagtgtagt taagacatga gtatacacaa gtagacttat actgacagat aaagcgatga
   147661 cttttttttc tctattgaat atcaatccat aagcataata taaaacagct gggataatcg
   147721 taagagcgat tgcttctgca attgcggatc gataatagat atcaactgtt cgataagttg
   147781 caaaagtata taaacaacta aataaaaaag cagcatctat ctttttaaaa aacttttctc
   147841 cataataaaa actaatcaga atcgtacaaa tatttaataa ccaaatatat agaacataac
   147901 ttaatattaa gttatttgaa attccataaa aaacgactgc aggaattatt gtaagaaaag
   147961 gataaaaata gttcagtcca tctccataat tgttaaatgt tgtatagttg attggccctt
   148021 caaaaataga agataaccct ttgattcgtg aaagatgaaa agataaatca cctgttgtat
   148081 aaatttcttg agcatttctc ataaaattta ctgaatataa agtactaagt aagataagaa
   148141 ctactaaaag aaatgctttt ttacttattt tcatagattt gttgttctcc ttttttaatt
   148201 ctattttcag tatactagaa ttgtcttttt ttaataagtt ttgcgttaaa tttttcat
//
LOCUS       DARBEC010000002        71619 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.002, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000002 DARBEC010000000
VERSION     DARBEC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.002"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            206..724
                     /locus_tag="SCL80_000131"
     CDS             206..724
                     /locus_tag="SCL80_000131"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1106032.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            852..1535
                     /locus_tag="SCL80_000132"
     CDS             852..1535
                     /locus_tag="SCL80_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEG1106033.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            1844..1917
                     /locus_tag="SCL80_000133"
     tRNA            1844..1917
                     /locus_tag="SCL80_000133"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Arg,seq:tct)
     gene            2076..2435
                     /locus_tag="SCL80_000134"
     CDS             2076..2435
                     /locus_tag="SCL80_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEG1106034.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            2558..4189
                     /locus_tag="SCL80_000135"
     CDS             2558..4189
                     /locus_tag="SCL80_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEG1106035.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(4251..4892)
                     /locus_tag="SCL80_000136"
     CDS             complement(4251..4892)
                     /locus_tag="SCL80_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1106036.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    5078..5154
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            5174..5482
                     /gene="rplU"
                     /locus_tag="SCL80_000137"
     CDS             5174..5482
                     /gene="rplU"
                     /locus_tag="SCL80_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEG1106037.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            5509..5853
                     /locus_tag="SCL80_000138"
     CDS             5509..5853
                     /locus_tag="SCL80_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEG1106038.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            5866..6159
                     /gene="rpmA"
                     /locus_tag="SCL80_000139"
     CDS             5866..6159
                     /gene="rpmA"
                     /locus_tag="SCL80_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEG1106039.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            6593..9400
                     /locus_tag="SCL80_000140"
     CDS             6593..9400
                     /locus_tag="SCL80_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1106040.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            9591..10082
                     /locus_tag="SCL80_000141"
     CDS             9591..10082
                     /locus_tag="SCL80_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1106041.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            10185..11174
                     /gene="manX"
                     /locus_tag="SCL80_000142"
     CDS             10185..11174
                     /gene="manX"
                     /locus_tag="SCL80_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEG1106042.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSVGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            11207..12010
                     /locus_tag="SCL80_000143"
     CDS             11207..12010
                     /locus_tag="SCL80_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEG1106043.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            12031..12945
                     /locus_tag="SCL80_000144"
     CDS             12031..12945
                     /locus_tag="SCL80_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEG1106044.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVQRWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            13028..13402
                     /locus_tag="SCL80_000145"
     CDS             13028..13402
                     /locus_tag="SCL80_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEG1106045.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            13541..13786
                     /locus_tag="SCL80_000146"
     CDS             13541..13786
                     /locus_tag="SCL80_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1106046.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            13958..15022
                     /locus_tag="SCL80_000147"
     CDS             13958..15022
                     /locus_tag="SCL80_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1106047.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            15268..15693
                     /locus_tag="SCL80_000148"
     CDS             15268..15693
                     /locus_tag="SCL80_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1106048.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            15686..16135
                     /gene="nusB"
                     /locus_tag="SCL80_000149"
     CDS             15686..16135
                     /gene="nusB"
                     /locus_tag="SCL80_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEG1106049.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            16232..17086
                     /locus_tag="SCL80_000150"
     CDS             16232..17086
                     /locus_tag="SCL80_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEG1106050.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            17087..18424
                     /locus_tag="SCL80_000151"
     CDS             17087..18424
                     /locus_tag="SCL80_000151"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEG1106051.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            18429..18653
                     /locus_tag="SCL80_000152"
     CDS             18429..18653
                     /locus_tag="SCL80_000152"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEG1106052.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            18655..19545
                     /locus_tag="SCL80_000153"
     CDS             18655..19545
                     /locus_tag="SCL80_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1106053.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            19554..20372
                     /locus_tag="SCL80_000154"
     CDS             19554..20372
                     /locus_tag="SCL80_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEG1106054.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            20479..20928
                     /locus_tag="SCL80_000155"
     CDS             20479..20928
                     /locus_tag="SCL80_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1106055.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            20946..22628
                     /gene="recN"
                     /locus_tag="SCL80_000156"
     CDS             20946..22628
                     /gene="recN"
                     /locus_tag="SCL80_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEG1106056.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(22683..22799)
                     /locus_tag="SCL80_000157"
     CDS             complement(22683..22799)
                     /locus_tag="SCL80_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEG1106057.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(22872..23828)
                     /locus_tag="SCL80_000158"
     CDS             complement(22872..23828)
                     /locus_tag="SCL80_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1106058.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            23999..24367
                     /locus_tag="SCL80_000159"
     CDS             23999..24367
                     /locus_tag="SCL80_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEG1106059.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            24537..24968
                     /gene="mraZ"
                     /locus_tag="SCL80_000160"
     CDS             24537..24968
                     /gene="mraZ"
                     /locus_tag="SCL80_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEG1106060.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            24989..25948
                     /gene="rsmH"
                     /locus_tag="SCL80_000161"
     CDS             24989..25948
                     /gene="rsmH"
                     /locus_tag="SCL80_000161"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEG1106061.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            25953..26354
                     /gene="ftsL"
                     /locus_tag="SCL80_000162"
     CDS             25953..26354
                     /gene="ftsL"
                     /locus_tag="SCL80_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEG1106062.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            26355..28547
                     /locus_tag="SCL80_000163"
     CDS             26355..28547
                     /locus_tag="SCL80_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1106063.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            28573..29535
                     /locus_tag="SCL80_000164"
     CDS             28573..29535
                     /locus_tag="SCL80_000164"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEG1106064.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            29549..30910
                     /locus_tag="SCL80_000165"
     CDS             29549..30910
                     /locus_tag="SCL80_000165"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEG1106065.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            30936..32024
                     /gene="murG"
                     /locus_tag="SCL80_000166"
     CDS             30936..32024
                     /gene="murG"
                     /locus_tag="SCL80_000166"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEG1106066.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFPENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            32039..33259
                     /locus_tag="SCL80_000167"
     CDS             32039..33259
                     /locus_tag="SCL80_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEG1106067.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            33415..34740
                     /gene="ftsA"
                     /locus_tag="SCL80_000168"
     CDS             33415..34740
                     /gene="ftsA"
                     /locus_tag="SCL80_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEG1106068.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            34763..36004
                     /gene="ftsZ"
                     /locus_tag="SCL80_000169"
     CDS             34763..36004
                     /gene="ftsZ"
                     /locus_tag="SCL80_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEG1106069.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            36021..36698
                     /locus_tag="SCL80_000170"
     CDS             36021..36698
                     /locus_tag="SCL80_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEG1106070.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            36712..37311
                     /locus_tag="SCL80_000171"
     CDS             36712..37311
                     /locus_tag="SCL80_000171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEG1106071.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            37326..37613
                     /locus_tag="SCL80_000172"
     CDS             37326..37613
                     /locus_tag="SCL80_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEG1106072.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            37662..38444
                     /locus_tag="SCL80_000173"
     CDS             37662..38444
                     /locus_tag="SCL80_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEG1106073.1"
                     /translation="MDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFLDPRQS
                     YILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYPSKFAS
                     LSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVAVRLEP
                     IKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNWTENTR
                     PDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            38465..39172
                     /locus_tag="SCL80_000174"
     CDS             38465..39172
                     /locus_tag="SCL80_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEG1106074.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            39434..42232
                     /gene="ileS"
                     /locus_tag="SCL80_000175"
     CDS             39434..42232
                     /gene="ileS"
                     /locus_tag="SCL80_000175"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEG1106075.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMSMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(42281..43807)
                     /locus_tag="SCL80_000176"
     CDS             complement(42281..43807)
                     /locus_tag="SCL80_000176"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEG1106076.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(43822..44469)
                     /locus_tag="SCL80_000177"
     CDS             complement(43822..44469)
                     /locus_tag="SCL80_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEG1106077.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(44653..44982)
                     /locus_tag="SCL80_000178"
     CDS             complement(44653..44982)
                     /locus_tag="SCL80_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEG1106078.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            45159..45887
                     /gene="sfsA"
                     /locus_tag="SCL80_000179"
     CDS             45159..45887
                     /gene="sfsA"
                     /locus_tag="SCL80_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEG1106079.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            45903..46916
                     /locus_tag="SCL80_000180"
     CDS             45903..46916
                     /locus_tag="SCL80_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1106080.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            46916..48193
                     /locus_tag="SCL80_000181"
     CDS             46916..48193
                     /locus_tag="SCL80_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1106081.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            48256..50958
                     /locus_tag="SCL80_000182"
     CDS             48256..50958
                     /locus_tag="SCL80_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1106082.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            51110..51427
                     /locus_tag="SCL80_000183"
     CDS             51110..51427
                     /locus_tag="SCL80_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1106083.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            51457..51777
                     /locus_tag="SCL80_000184"
     CDS             51457..51777
                     /locus_tag="SCL80_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1106084.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            51885..53345
                     /locus_tag="SCL80_000185"
     CDS             51885..53345
                     /locus_tag="SCL80_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106085.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGTLPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGPNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            53413..53634
                     /locus_tag="SCL80_000186"
     CDS             53413..53634
                     /locus_tag="SCL80_000186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106086.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            53665..53847
                     /locus_tag="SCL80_000187"
     CDS             53665..53847
                     /locus_tag="SCL80_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEG1106087.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            53847..54260
                     /locus_tag="SCL80_000188"
     CDS             53847..54260
                     /locus_tag="SCL80_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEG1106088.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            54383..55564
                     /locus_tag="SCL80_000189"
     CDS             54383..55564
                     /locus_tag="SCL80_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106089.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(55635..55799)
                     /locus_tag="SCL80_000190"
     CDS             complement(55635..55799)
                     /locus_tag="SCL80_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106090.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            55953..56026
                     /locus_tag="SCL80_000191"
     tRNA            55953..56026
                     /locus_tag="SCL80_000191"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:55987..55989,aa:Arg,seq:cct)
     gene            complement(56063..56263)
                     /locus_tag="SCL80_000192"
     CDS             complement(56063..56263)
                     /locus_tag="SCL80_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1106091.1"
                     /translation="MKINYTVDLVSFSLVINFSLFSLRLFIDGLRHTHASILLYQGVN
                     ILSVSKRLGHSSLETTMSTYTK"
     gene            complement(56568..57800)
                     /locus_tag="SCL80_000193"
     CDS             complement(56568..57800)
                     /locus_tag="SCL80_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEG1106092.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            58054..58623
                     /locus_tag="SCL80_000194"
     CDS             58054..58623
                     /locus_tag="SCL80_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1106093.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(58801..59241)
                     /locus_tag="SCL80_000195"
     CDS             complement(58801..59241)
                     /locus_tag="SCL80_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEG1106094.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            59399..60163
                     /gene="map"
                     /locus_tag="SCL80_000196"
     CDS             59399..60163
                     /gene="map"
                     /locus_tag="SCL80_000196"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEG1106095.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            60195..61175
                     /locus_tag="SCL80_000197"
     CDS             60195..61175
                     /locus_tag="SCL80_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEG1106096.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKSRNKSSYCKFISVIYNNYDS"
     gene            61193..62332
                     /locus_tag="SCL80_000198"
     CDS             61193..62332
                     /locus_tag="SCL80_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEG1106097.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIVLIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            62325..63125
                     /locus_tag="SCL80_000199"
     CDS             62325..63125
                     /locus_tag="SCL80_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1106098.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            63125..63952
                     /locus_tag="SCL80_000200"
     CDS             63125..63952
                     /locus_tag="SCL80_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1106099.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            63930..64664
                     /locus_tag="SCL80_000201"
     CDS             63930..64664
                     /locus_tag="SCL80_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1106100.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            64764..65630
                     /gene="rfbA"
                     /locus_tag="SCL80_000202"
     CDS             64764..65630
                     /gene="rfbA"
                     /locus_tag="SCL80_000202"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEG1106101.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            65644..66216
                     /gene="rfbC"
                     /locus_tag="SCL80_000203"
     CDS             65644..66216
                     /gene="rfbC"
                     /locus_tag="SCL80_000203"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEG1106102.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            66238..67266
                     /gene="rfbB"
                     /locus_tag="SCL80_000204"
     CDS             66238..67266
                     /gene="rfbB"
                     /locus_tag="SCL80_000204"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEG1106103.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTSILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWQPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            67364..68215
                     /gene="rfbD"
                     /locus_tag="SCL80_000205"
     CDS             67364..68215
                     /gene="rfbD"
                     /locus_tag="SCL80_000205"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEG1106104.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLPYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEALGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            68250..70283
                     /locus_tag="SCL80_000206"
     CDS             68250..70283
                     /locus_tag="SCL80_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106105.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFSLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMIAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFVALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            70327..71607
                     /locus_tag="SCL80_000207"
     CDS             70327..71607
                     /locus_tag="SCL80_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEG1106106.1"
                     /translation="MKIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGVGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LVILFCLVIKWIEWTKWDRGIYLIVFTVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNTIGSSLMTLALLISGFASSFERWYVRIIGGIAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTDREDIWHKFY
                     QTLGEKSEQVLLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
BASE COUNT        23291 a        12146 c        15326 g        20856 t
ORIGIN      
        1 attcctttct aaaacttgta atgttagggt aaacgttttt taaactaacg tcaattattt
       61 ataataaaaa atgagataaa gatgaggaaa tagaattaga aacatataat tattaaaggg
      121 aaacattttt acagctataa tttgaaaaaa atttatcgtt attatatgat taagaaggat
      181 tgaataaaga aagaagttga aaacgatacc tgattacaaa aaaataactt ttataagttc
      241 tataacgttg attatatttt taattcttat tcttctcgtc gtcttggttc cccgaatttt
      301 tggtttgact ccttatatta ttgaggacaa agaaatgagt ccatattatg cacaaggaaa
      361 tctgatatat gtacgaccga aaaatacaaa tgagatttta gtcggagata ttattacata
      421 ttatgaaaat tcaggaaaaa aaatctcgac gcgtcgcgta atagccgttg aaaacaacca
      481 cgaagatttt tatacaaaag ctgatacaaa gacttatatt gagcctggag tagtaaaaaa
      541 gagaaatatt atcggtagtc ccatctttca gattccatat attggactag tcctttcaaa
      601 aacggcattt gcatggattc aggggttatt ctttacgatt gctttttgcc taacaggaat
      661 cacagcatgg aatacctttg tagagttgaa aaagaagcgg agacgaacac agaaattttt
      721 gtaagaatgt taaaaacata aatgtattcc agagatccaa cgactcttct gaacatgaaa
      781 cacgagaaaa cactcatgca gcttgtagat ttagtcagtt gttcgtataa tgtgtgaaga
      841 ggaggagtag tatgcgcaat gaaatgatgc atcgtcctgt agtcaaagaa gaactattgg
      901 attttatgcg tacccagcaa aaaaaattac cgggtgagct aggcaaactg gaagaagaag
      961 ctcatgtagc agaagtacca gttattcccc atgaaacagt tgtatttttg aaatttttac
     1021 ttggacagct tcagcctgaa cgaatcttgg agatcggagc agctatcggt ttttcatcaa
     1081 gtgtcatggc cacgacaatc ggagaaaacg ctcatgttac gacaattgat cgtttcgatg
     1141 tcatgatcga gaaagctaaa aaaaactatg aacgtctagg actgacagat aaagtgactt
     1201 tgcttgaagg tcaagcagca gatatcttgc cggagttgtc tggtccgtat gactttattt
     1261 ttatggatag tgcaaaatca aaatatattg aatttttacc tgaatgctta cgtttgttaa
     1321 gaaaaggtgg cgttttgatg gtagatgaca tcttccaagg aggaacgatc ttgctcccag
     1381 atgaagagat tccccgtggc aaacgagcta tccatcgtaa attaaatgaa tttcttcgtg
     1441 ttgtgatgga tcatcccgat ttgacatcga caatcttgcc attaggtgat ggtgtgatct
     1501 taatgacaaa ggaatcagaa acaatcgagt tgtgatacat gattatggca gcctgtagat
     1561 aatcgattga atcgagggct caattcgaac ttgatgtaaa agtttctata tttgtaagtc
     1621 aattaaagga tacaggaaat ctaaataaac aggagtagca tgaagtcaat gtagctcctg
     1681 ttttcaattg ataaccttag aacagctgaa ggagaatgtc tcgttttaaa agattgattt
     1741 agtagttggg ccaaaataat tcattatttg ctaattaaat aactatttcg tattgacaga
     1801 agcaatttaa ttatctataa ttattgatgt tccgctgatt cttgtcatag tagctcagct
     1861 ggatagagca tccgccttct aagcggacgg tcgggggttc gaatccctcc tgtgacgcta
     1921 aaaaaccgtt agaaactgag gtttctaacg gtttttcttt gttttttgtg ccaatattgt
     1981 ttgttttcaa aggcaaataa aataaaaaaa catccttatc gagtacaatt ataaggagca
     2041 aaaaaataaa actcatcaga aaagaggaag aagatatgac gatactatct agaattttag
     2101 tgactctagt tgcaattgaa tttttttata tcatgtatat cgaaacggtg agaactgatt
     2161 ctgacacgac aagtagggtt tttaaaatga gtaaagaaga gttaagcaga aaatctgttc
     2221 aaacattgtt taagaaccaa ggagtgtaca atggactatt aggagtggga ctactttatg
     2281 gagcttatct ttcttccgca tctaaagaaa tcacaagtat gctattaata tctatttttt
     2341 ttgttgcatt atatggcagt ttgactagcg atcgttggat tttactcaaa caaggtgggc
     2401 tagctctgtt ggcattgctt tctattttct tataaatata gaaaaaataa gaatttatct
     2461 aacaggaaac tttgtgaaaa atattacttt atctcatgaa atatgatatt ctaatgaatg
     2521 gtattttaga aaatttcatg aaaaaggagt tagataggtg ttcaaaaatt atgtttcctc
     2581 actaaaaaaa gaattttcag gttatgatct ttcaagattt cagaaagatc ttttggctgg
     2641 gatcacagta gctgctgtag cattgccttt agccttggct tttggggtat caagcggagc
     2701 tgatgcggcc gcaggtttga ttacggctat cattgccggt atcgttattg gcggtttatc
     2761 aggcggtttt tatcaaatat caggtccgac aggtgcgatg gcagctatct tgatgtctat
     2821 tgcggcaaca aagggaatgc aaggtgtatt gcttgctact tttttagcgg gtctattttt
     2881 acttgcagca ggacttttcc gattaggaac actgacttct tttatcccag cacccgtaat
     2941 tacggggttt acttcaggta ttgctatcat tattgcatta ggtcagatcg ataatttatt
     3001 tggtgttcac tcggaaggta caaacgttat tgaaaaacta agtagttatc aaacgatagg
     3061 atttccgata aacacagcct ctttgatcat gggagggcta gtcattttag ggatgtttat
     3121 ctatccaaaa aaatgggcga aacgaatgcc tagttcgctg ctggctatca ttttggcaac
     3181 agcagttatg ctattgttcc atttaccagt agcaactgtg ggtaagattc cacaaacatt
     3241 gatcagtagt aatcgtttga atatgggaga ttttagcttt tctgccttgc aacaagtggc
     3301 agttccagct attagcattg ctttattagg aatgattgaa agcttgctat gtggtgcttc
     3361 ggctggccga atggcaaata agcccttaga tagcaatcaa gaattaatgg cgcaaggaat
     3421 tggaaatctg ttgcttcctt tctttggcgg gattcctgct actgcggcca tcgcccgtac
     3481 aagcgtggcc atcaaatctg gtgcacaaac tagattagct ggtatgatac atgcagttgt
     3541 cttactgctc tccatgcttg tttttgcgcc agtgatgtca cagattccaa tgccagcact
     3601 tgcaggtgtt ttgattgtga cggcttggcg tatgaatgaa tgggaaacga tcaaggagct
     3661 atttactaat aaatattggt cagcagttct attgtttatc cttacgatga tttgtacagt
     3721 tatctttgat ttaagtattg ctatcgtgat cggggtgatc ggcggctgtg tattttttgt
     3781 tgtcaaaagc gctgctatca cgatttcatt agaaacgatc gattggcaaa gaatggggct
     3841 gccagaaacg gaaaagctga ataactggac agtagtgtat attagtggtc cactgttttt
     3901 tatgtcttca gaaaaactca aaaataaatt ggaagaacta aatcatcacg atggtgtcat
     3961 tttctccatg cgaggcgtac caagtattga tttgacggca cgtgcagttt ttgatgaatt
     4021 tcaagaaaaa gcagagcaaa gaggacaaac gattatctat acttccttgc agccagaagt
     4081 agaaaaacaa ctttctcact tgtgggaaca tacattcacg catcaacatc aaacagttgc
     4141 tcatgcgttg acagctttac atgcgaaata tactgcaatt agccattaaa acaatgaaaa
     4201 agaacagaca agactgtaaa ctgaagattt tatggtcttg tctgttcttt ttaatattgc
     4261 tgtaaaagtg aagctagtac ttgccagtct tgatggataa caggtacaag ggagcgattg
     4321 tagaaaatag ctgctggatg aaaaagtgga agaattcgat agtttttttc tgaccattcg
     4381 taggcatcct tttgttctgt taattgtaag acaggccctg tatagagttg accatgattt
     4441 ttggtaaccg tatattcttt gccaagtaaa cgttgaagac caatgtttcc aagtgtcgca
     4501 attaattttg gctggacctt catcagttcg taatccaata ttggtgcatg cgcaaatact
     4561 tcggaacgag taggtgtccg atttggataa acagtttctg actgttgtgt ccgtttattg
     4621 atccgatgag tcacgcgata cgggcggctc cttacagcac tggtgatata cacctcttct
     4681 cgtgttaagc cagttgaaga aagtgcctgc atcaattctt ttccagcttg accgctaaaa
     4741 ggaacatgcg tatcaatctc tgttttccct ggggcttctc ccacgagcat cagtttagga
     4801 tgaagaggtc cttgcccagc gataaatcct tctacaggac ggccgttcat tcttaaagaa
     4861 gcttcttgta tcagttcttt tgggtattcc atttatgctc ctcctttttc ttttattata
     4921 agaataaaag aaacacaatg cgactatttt atttggtaga actgtgagga tagaaaagca
     4981 gaagtattgc ttgaattttt gttttacttt ttgattttaa atttagttga ctaacggttg
     5041 ctgttctggt aagctagtac tgttgtaatt gtggactcca cagctacaac cgctcagaac
     5101 aagtaataag cacagcatat gctgtcactt cggatggcga gtctaagtat atcagaagga
     5161 ggtgctttat agcatgtacg caattattaa aactggtggt aaacaagtaa aagttgaagt
     5221 gggacaagca atctatgttg aaaaacttga cgttgaagca ggagaaaaag ttgtttttga
     5281 cgaagttatc ttagtaggcg gagaatctac aaaagtaggc gctccaactg ttgcaggtgc
     5341 aactgttgaa ggtactgttg aaaaacacgg caaacaaaag aaagtcgtta ctttcaaata
     5401 caaacctaaa aaacattcac accgtaaaca aggtcatcgt caaccatata caaaagtggt
     5461 tatcgacgcg atcaacgctt aatagcgttt gagaaagaag gcctatatat gatcaaagga
     5521 acatttaagc gaaatgacgc aggtcagatc gtttcattca ctttgaccgg gcatgctgat
     5581 gctggtcctt atggaagtga tatcgtttgt gcgggtgttt ctgcgttagc gatcagcact
     5641 gtcaacggga ttgcctcatt agccggtttt gagccgattg ttgaaatgaa tgaagaagaa
     5701 ggcggttatc tttataccga agtcacttca ggaatgacac aagaacaaaa caatattgct
     5761 gagattctat tagaaaatct tttattagga ttacaatcga tagaagctga aaactcagaa
     5821 tatatccaaa ttaaaacaat taatgataaa taggaggtgc agaccatgtt gttatctatg
     5881 aatctacaat tatttgccca caaaaaaggt ggcggttcta catctaacgg ccgtgactca
     5941 gaatcaaaac gtctaggtgc taaacgtgct gatggacaaa cagttacagg tggatcaatt
     6001 ctttaccgtc aacgcggtac aaaaatctat ccaggtgtaa acgtgggtat cggtggagac
     6061 gacactttat ttgcaaaagt tgatggcgta gtacgtttcg aacgtaaagg ccgtaacaaa
     6121 aaacaagtgt ctgtttatcc agtagctcaa gaagcttaat tgaatcttat gattaaagag
     6181 gttgtgacaa gacttttgtc acagcctttt ttgtttataa aaataaaagt tgtagtggag
     6241 gctacgaagt ttactttgtc tttttacact taatattttt ttgccaattt ggaatcagga
     6301 tgttttgata actatcgttt tcaattgatg gaagctatat tgtttgctat cagtgaaata
     6361 attatatggt aaacagtgtt ctttttcttg tttccattat aaaaatcgta tcgggatttt
     6421 tcgttctttt aaattgaaca tttctatcta tccgcgtgtt atcaacaaaa caaaatcagg
     6481 taaatagttg acgaaacaga aataaataca tagtattatg ttttgaagcc taagtacaat
     6541 agtctataga aaaggagcga taccattggc aaaacgcatt gaccgaattt atatgtatgt
     6601 caaagaaaaa accgctcatt taacaaaggc agaatatgat caaggagtaa caacacagga
     6661 aattgctgaa gtgttaggga ttcagcgaac aaatagcagt aaagatctga atcagctagt
     6721 gcgcgaagga aaactgataa agacagacgg acgaccagta cgttacatat atcaaacact
     6781 cgttacgcac gtaaagccag ttacaaaaca tgtagtcagt tacaaagaaa cgccggtctc
     6841 tgaagaaaaa aatacactcc aaatagatac taaggatatc tttgcaaaaa tcattggagc
     6901 taacggcagc atgaaaaatt cagtggaaca ggcaaaagct gcgattttgt atccgccaag
     6961 aggattaaat tgtttgatta caggacctac cggatcagga aagacttatt ttgcacacgc
     7021 gatgtatcat tttgcaaaag cgcatcatgt aatagatgag gaaacggaac tgattgtttt
     7081 caattgtgct gattatgcca ataaccctga gcttttgatg agtcatttat ttgggtacgc
     7141 aaaaggtgct tttacaggcg cagatgaaga aaaaatgggg attatcgatc aagcggatgg
     7201 cggtatgctg tttttggatg agattcaccg gttgccgcct gaaggtcaag aaatgatctt
     7261 ttactttatg gatcatggaa cttacagtcg tcttggggaa acaacgaaat cccatgaagc
     7321 aaatgtacgg atcgtaggtg cgaccacaga agacccaggt tcttctctat tagaaacttt
     7381 tgttcggcgt atcccaatca atatcaaact tccggctttt gaaaaacgtc ctgcaaatga
     7441 gaaagtcgat ttagtaaaaa tcatgattgc ccacgaagca aaccgcacgc agcgaaagat
     7501 ttctttgaca gaagatgtag caaaagcatt gatcggcagt gtgacctatg gaaatatagg
     7561 acaattgaag tcaaatatcc agttagtatg tgcacgagga tttctaaatc atatgcaatc
     7621 acccgaaatc tcgatcacaa tcgatgattt gacagaaggg attcgtagcg gattgattca
     7681 attagcaagc aaccgcagtg caatggcaga gctttctaaa atattggagc caaagataac
     7741 tgtagcacca aatgatacga tcatgaaaat ccaatcggac tcctatgagt taccttataa
     7801 tctatatgat atcatcggag ataaagcagc tttactgaag tcggatggtt tggatcaaga
     7861 agcaatcaat catttcattt ctacagatat caacatccat ctgaagtctt tctataaaga
     7921 ccatggtttt tcttttaatg cagacagcaa attagcagaa tttgtagatc cgaaaatcat
     7981 agaagtgacc aatcagattt atgtaatggt caaaagatct ttaccatatg agtttcagca
     8041 aaactttatt tatgctatga gcttgcatat cagttctttt ttgaaaaaaa tcaacttagg
     8101 agaagaacgg catacgaatg ataacatccg tgagatggct tttgattttc cagatgaata
     8161 tgctgttgcg aaagaagtgc gtagttatat cgaaacgtat tttcaagtgg agattccaga
     8221 aagtgaagac tattacttga ccgttctact cgtctcgtta cgggcaaatc aagcaagcgg
     8281 cagaatcggt gtagtcgttg cagctcacgg aaacagcaca gcaagcagta tggtccaagt
     8341 tgtccaacag ttgttagatg cagagaacgt acgagcagta gacatgcctt tggatatgga
     8401 tccgaaaaca gcattggacc gaattgaacg caatgtacaa gaagtagatg aaggaagcgg
     8461 ggtcatactc ttagtagata tggggtccct tgcgtcgttt aattcgcaaa ttcaaagaga
     8521 cactggaatt ccagtacgta ctgttgatat ggtaacaact tctctagttt tggaaactgt
     8581 acgaaaggtc tcagtattag ggacagatct agatatgttg catgaatcat tgaaaaattt
     8641 tcgaggttac gcagaaatat cttctgtaga atctgttcag gaagcaaaac ctattcataa
     8701 gaaaaaagct gttttggcta tctgcgcatc tggcgaaggt acagcacaaa gaatcaaaga
     8761 attgatcgaa cgagcggtaa acaagcgtca agaaacagaa ttagaagttc tagctttgtc
     8821 gattgtggaa gtgaaagaag aactgccagt tatccaaaaa gaatatcaga tcatagctac
     8881 tactgggatt actgatttga atatccaagc cccatttatt cctttagaac gttttattga
     8941 tcaaaatatc gaagtcattt tggatcagtt gctgatggaa tcagaattgg aggaaactga
     9001 attcatctcc ctagatgaag agtcagctaa aaatacgtgt gttgagttta tctctgataa
     9061 cttcatcttt attaatggta gtaagttaat cgatccaatg tggcaatttt ctacacaaat
     9121 ctctcagacg acgggaatcg gcgatgaaga atatggattc aaaatcaacc tagtgatgca
     9181 cacagcagga atgatcgaac gtattattcg gaatgaacca ttgactgtgg aagaaaacga
     9241 attgacaaat acaacgaatg atcctcttta tagccaattg gcagcctcag ttgttctgtt
     9301 ggaagatcag ataaaagtaa aagtgccgat agaagaaatg tattatctgc tacgtctagt
     9361 ccataatcag ttagataaaa aagaatacac tgtgccatga atacacaaaa acgatacact
     9421 tttatagtgt atcgtttttg tgtattggtt aagaatcctt taaattcaat tagtgtatcg
     9481 taaaaaagtt ggcacgcttt ttgcttatag tagtgtgtgc taggaggtgg ttgttcacac
     9541 agaaatgata ggggtttctg tgaagtagtg aggaagaagg agttttgcag atggatattc
     9601 gattggcacg tatagatgat cgtttaatcc acgggcaagt cgcaacagtt tggtcgaagc
     9661 aaacaggtgt tgaacgtatc attgtgatca gcgatgtagt cgctcaagat aaattgagaa
     9721 agtttctact aaaagaagct gcaccgccgg ggatcacagc aaatgtaata accgtgcaga
     9781 aaatgattca aatctatcat agtggattat tatcacgagc aaaagtgatg cttttattca
     9841 ccaatcccca agatgttgaa gcggttgtcc gctgcggtgt acagttgaag tcgttgaata
     9901 tcggcggtat gagttttact gaaggcaaat caatgattac caattttgtt tcagtaaatc
     9961 aacaagatat tgattccttt cgttacttga acagtcaaga agtggagtta gagattcgaa
    10021 aagtacctgc tgacaggaaa atccttttga tggaactact agcgaaaatc aaaaaggtgt
    10081 agcaagaatg gttgttggaa aaggtcaaat aattctgttg taatggttag caattattaa
    10141 tatttttgaa tttgttacaa caaaagtaaa taggaggtat aaatatggta gggattatcc
    10201 tagcaagtca tggacaattt gctgaaggaa tcttacaatc cggttcaatg atctttggcg
    10261 aacaagaaaa cgtcaaagcc gtgattttga aaccaagtga aggtccagat gacttgagag
    10321 ccaaattgga agaagcggtt gcttcttttg ataatcaaga cgaagtgtta ttcttagttg
    10381 atttatgggg cggaacaccg ttcaatcaat caaacacatt attcgaagag cacaaagata
    10441 aatgggcaat cgtcagcggt ttgaatttac caatgttgat tgaagcctac gcatcacgct
    10501 tttcaatgga atctgctcat gaaattgcag cacacatcat tgaaacagca aaagacggtg
    10561 taaaagtaaa accggaagaa ttagaaccgg cagaagcacc gaaagcagca gttgaagacg
    10621 cacaacctaa aggagcactt ccagaaggaa ctgtggtcgg agacggaaaa atcaaatatg
    10681 tattagcacg tgttgactca cgtctgttac atggacaagt tgcgacagct tggacaaaag
    10741 cagtgcaacc aaaccgtatt atcgttgttt cagatgcagt atcaaaggat gatctgcgta
    10801 agagactaat cgaacaagct gctcctccag gtgtaaaagc aaatgttatt cctattagca
    10861 aaatgatcga agtagcaaaa gaccctcgtt ttggaaatac caaagcattg cttttatttg
    10921 aaaatcctga agacgtattg acagctgttg aaggtggcgt agacatcaaa gaattaaatg
    10981 ttgggtcaat ggcgcattcg gtcggtaaag tcgttgtaag taaagtattg tctatgggac
    11041 aagaagacgt tgaagcgttt gagaagcttg aacaaaaagg tgtgaaattc gatgtacgta
    11101 aagtacctaa tgattcacgc gataacatgg atgatatttt gaaaaaagca aaagctgaat
    11161 tagcaaaagc gtaataaata atattaaaat ttataggagg cttatcatgt ctattatttc
    11221 aataatttta gtcgtattag ttgcctttct agctggtatg gaaggtattc ttgatgaatt
    11281 tcaatttcat caaccactag tggcatgtac attgatcggt ttagttaccg gtaacctcga
    11341 agctggtatc gtacttggcg gtacgcttca gatgatcgct ttaggttggg caaatatcgg
    11401 tgccgctgta gcaccagatg ctgcattagc atcagtagca tcagcaatta ttttagtttt
    11461 aggtggacaa ggtgttaaag gtgttccttc tgcgatcgcg attgccgttc ctttggctgt
    11521 tgcagggttg ttcttaacaa tgatcgtacg tacaatcgct gttccaatcg ttcatttgat
    11581 ggatgcggct gctgaagaag gaaacatccg taaggttgaa atgtggcaca tcattgctgt
    11641 atgtttacaa ggggttcgta tcgcggtccc agcagcagcg ttgctattta tcccagcaca
    11701 aactgtgcaa tcattccttg aatcaatgcc agcatggtta acagatggta tggctatcgg
    11761 tgggggaatg gtcgtagctg taggttacgc tttggtaatc aacatgatgg ctacaaaaga
    11821 agtatggcca ttcttcgtta ttggtttcgt tgtagctgca atttctcaat taacattgat
    11881 tgctctaggt gctttaggtg ttgcattagc tcttatttac ttgaaccttt ctaaaatggg
    11941 tggttcatca aataatggag gcggaagcaa ctctggtgac cctttaggcg acattttgaa
    12001 tgactattaa tcttgaagga ggaaaataaa atggcagaag aaaaaatcaa attatcaaaa
    12061 aaagatcgtt tagctgttgc gtggcgctct accttcattc aaggttcatg gaactacgaa
    12121 cgtatgcaaa acggtggctg ggcattttct atgattccag caatcaaaaa attatacaaa
    12181 acaaaagaag aacgttctgc agcattaaaa cgtcacttgg aattctttaa tacacaccca
    12241 tacattgctt ctccaatttt aggggttaca ttggctctgg aagaagaacg tgcgaatggc
    12301 gcgcctgttg atgatgtagc gattcaaggg gttaaagtag gtatgatggg tcctttagct
    12361 ggtgttggtg acccagtgtt ctggtttaca gtacgtccaa tgctaggtgc actaggtgct
    12421 tcattagcta tgggaggaaa tatccttgga ccaatcatct tcttcttagg ttggaacatt
    12481 attcgttggg cattcatgtg gtatacacaa gaatttggct acaaagctgg ttctaaaatt
    12541 actgatgacc tttctggcgg attgttacaa gacgtaacaa aaggtgcttc aattttaggg
    12601 atgttcgtat tagctgccct tgtacaaaga tgggtatcaa tcaaattctt accagttgta
    12661 tcaactgtaa aattagataa aggtgcttac atcgaatggg ataaattgcc ttcaggtggt
    12721 gaaggaatca agagtgcatt tgaacaagtt aataatggct tagccctttc tcctgaaaag
    12781 gtaacaacat tacaagacaa cttagaccaa ttgattcctg gtttagctgc attaggatta
    12841 acattgttat gtatgtggtt gcttaagaaa aaggtttctc caatcgttat catccttggt
    12901 ttattcgtag tcggtgttgt tggacacgtc atcggacttt tataatccat aaaaatatag
    12961 ctcgggcgtt gtcccgagct ttttttatat agtatactgt tcataaacaa atatctggag
    13021 ggagaaaatg gttcaatcaa tcaatacaaa agtagacttg gtaattgatg ctacttcgtt
    13081 tacgggtctg acagattacg ggcaaatcat gataggtgat aatggttttg agttttacca
    13141 tgcgcgagat agacgcaaat atatccaaat tccatgggaa gaagtggatt atgtactagc
    13201 atcagtcatg ctaaagggaa aatggattcc tcgatatgcc attcaaacga aaaaaaatgg
    13261 tacatacaca ttttcttcca aagaacctaa aaaagtgctt cgtgcgatca gagaatatgt
    13321 agatgaagat agaatggtac aatcattgag tttctttgat gtagtgaaac gtggtattcg
    13381 tagtatgttt aaacgcaagt aaaaaataaa gaaaacttct aagaggaggt tttctttatt
    13441 tttttacttt gcagaagaaa atatgaaata gcaaaaccaa ttatttgtcc cctttaaatc
    13501 ccatatgcta aaattgatta gcaaagaagg aggttatatt atgagttggt tatggacatt
    13561 gattgttgga gccgtgatcg gagcaattgc aggtgcgatc accagtaaag gaaattcgat
    13621 gggctgtgta acgaatatca ttgctggact agtcggttca agtattggac aagcgatttt
    13681 cggcgaatgg ggaccaaaag ctgcaggtat ggcacttatt ccatctattt taggcgcagt
    13741 gattttggta gcagttgtct catttttctt tggaaaacga tcataagttt ttacaaaata
    13801 gtaaactaaa aggagttgca gcgagactgt gactcctttt tggctttatt tatttaaaat
    13861 aaaaagaagt tttccggaaa tttttattta actgtcgtgt acgaagaaaa gtttgctata
    13921 atggaaaaaa gcgtgcaaag aaagagggag tttcattatg atgctacgtg tagaaaaatt
    13981 gcgcaaaaaa atgcaagaag aaaatttgga ttcatttttg gttaccagtc cgtacaatct
    14041 tcgttactta acgaacttta caggtacgac aggattagct gttattactt tggaaaaagc
    14101 attttttatt actgattttc gttataccga gcaggcggca gcgcaggctc aagggtttga
    14161 aattattaaa aatgtcggac ctatttttga tgaggtagca aacttagtac gcaaagaaga
    14221 actgcactca ttagcttttg aggaaacaac agtctcattt ttagaatatt cggttttaga
    14281 agaaatcatc gatgcggaat tagtacctgt ttcacagatg atcgaagaac tcagagaagt
    14341 gaaagatgaa gaagaaatcg ctattatcga aaaagcatgt catattgcgg atatggcata
    14401 tgatcacatc ctgaaaatga tccaaccggg catgactgag attgaagtcg caaatcaact
    14461 agacttttat atgcgttctc ttggcgcttc aggtgtttct tttgatacga tcgttgctag
    14521 cggattgcgt tcggctatgc cgcatggtgt agcaagcaaa aaaatcatcg aacaaggtga
    14581 cttgatcaca ttggactttg gttgctatta cgaagggtac gtttccgata tgacacgtac
    14641 attcgctatt ggtgatcctg gggaaaaatt aaaagaaatt tatcaaatag tcttagatgc
    14701 tcaattagca gttattgatg ctgcaaaacc aggaatgact ggtgtccaat tagatgcagt
    14761 tgctagagat tatatttcaa aacatggtta cggggaagca tttggacatt ctacaggaca
    14821 tgggatcggt ttagagatcc acgaaggacc aaacgtttct tctcgtgcag aaaagcaatt
    14881 tgtaccagga aatgtgatca ctgatgaacc agggatctac ttaccaggaa tcggtggagt
    14941 acgtattgag gatgacttgt tgatcacaga agatggaaat cgtgtgttga ctcgttcacc
    15001 aaaagaattg atcatcttat agtttcatca atagaagaag taagcgtaaa aatcagtgtt
    15061 cacagacttt gttatagaaa atcactcgat ttaatgatac actatagaag aacttttaag
    15121 ctgtaagaga taataaagtt gggtgttttc tccaattcat agcagtttgc agctgatcgg
    15181 cggcagtttt cttaccacgt gatcactatg ccgcatcata gagaattcgg atgtatctcg
    15241 tgcttaggca aagggaggaa taaataaatg gcagatgaaa aaaatttgat tttaaacgca
    15301 aaccaagatt taggagagat cgtgatcgct cctgaagtca tcgaagtaat tatcgggatt
    15361 gcagcttcta aagtagaagg cgtatacgga atgcgcggaa cttttgccag caatgtcact
    15421 gaattattag gacgtgcagc tcacggaaaa ggtgtttact tagcaaacga ggaagatggc
    15481 ttgaaagtag atctttactg ttatctagaa tatggtgttt cagttcctaa agtagcaatg
    15541 gaaatgcagg aacgggtcag ccagcaagtc cttttcatga cggatatcga tttatctgaa
    15601 gtcaatatcc atgtagtagc tgttgttcct gaaaaattag aacaaccaac aatcgaagaa
    15661 ctttttgaag acgaagagga agagaatgaa taaagaatta tcaagacacg agattcggga
    15721 aatggcattg caggctttat ttccattaga cttcaatgca gacctaacaa aagaagatgc
    15781 gattttcaac gcaatcgaat tagaccaccg cgacatgatc aacgaagatg aatcagagtt
    15841 tgttcctgtt tatcttgata cgctagttgg cggtgtctgt gcaaaaaaag atgaattgga
    15901 taaagtgatt gaaaaacatt taaaaaacaa ttggcacatc aatcggatct caaaaatgga
    15961 tttggtgatc ttgcgtatcg cgatttttga aatgacttat gtgacagatg taccagcggc
    16021 tgttgcttta aatgaagcaa tcgagttggc aaaaacattt agtgatgacc gttcacgtaa
    16081 attcgtcaac ggtgttttat ccaatgtatt gaaagaatta gaagctgggg cgtaagtcct
    16141 agcttttttc tatgaatcat cataaacctg ttataaatcg ggaaaattct atggtagaat
    16201 gaaaacacaa aaaatatctg ggagtggtta gatggcagaa ttgattaacg gaaaagaatt
    16261 agctgaaaaa atgcaggcag agattgctga aaaaatccaa aaattgaaag ataatggtat
    16321 ccatcccgga ttagtcgttt tattggtagg ggaaaatcca gccagccaaa tctatgtaag
    16381 aaataaagaa cgtgtagcaa aagaaatcgg aatccattca ttggtagagc gttatcctga
    16441 gacgatttct gaagacgaac tacttgcaga aattgaaaaa tacaatcaag atcctagttt
    16501 tcatggtata ttggtccaat taccattgcc aaagcatata gatgaagaaa agatcctgtt
    16561 agctatccgc cctgaaaaag acgtagacgg ttttcatccg atgaatttag gcagactcct
    16621 tgcgggtaaa ccggacaaga ttccttgtac tccatacggc atcatgaaaa tgtttgaagc
    16681 ttattctatc gatccggcgg gtaaacgagc agtagttatc ggacgaagca atatcgtcgg
    16741 taaaccgatg gctcagctac tcttgatggc agatgcaact gttactgttg ctcattctaa
    16801 aaccgaaaat ctggctgaac tggcacaaga agcagatatt ttagtcgtag caataggacg
    16861 tggacatttt gtcacaaaag aattcgtaaa accaggtgct gtagtgatag atgtagggat
    16921 gaatcgtgat gctaacggta agctgatcgg tgatgtgaaa tttgatgaag tggagcctat
    16981 tgcaggctgg atcactcctg tgccaaaggg tgtagggcca atgactatca cgatgctgat
    17041 gtatcaaacc gtaaaatctg ccgaagcttt tggagcaggt gaatagatgg cacaagaata
    17101 tttaactgtc actacattaa cgaagtattt gaaaagaaaa tttgatgccg atccttattt
    17161 agaacgcgta tatttaacag gcgaaatatc gaatttccgt ttacgtgcca atgcccatca
    17221 atatttcagc ttaaaagatg accatgcaaa aatatctgct attatgttca aaggagcatt
    17281 ccaaaaatta cgattccagc caaaagaagg aatgaaagtc cttgtcgttg ggcgtatttc
    17341 attatatgaa gcgggaggtt cttatcaaat ctatgtagag cacatggagc ctgatggaat
    17401 aggtgcttta tatcaagaat tggaagaacg aaaagaaaag ctgatgaagg aaggcctttt
    17461 tcaagggcct aaaaaaatgc tgccacgttt cccaaagcga atcgcggtcc ttaccagtcc
    17521 aagcggagcg gttatccgcg atatcatcac gacggtgaaa agaagatatc caattgtcca
    17581 attggttttg ttcccaacag ttgtacaagg aaaccaagca gcggacaatg ttgtcaggaa
    17641 tatccagaga gtagaagaga tgggcaattt cgatacaatg atcatcggtc gtgggggtgg
    17701 ttcgattgag gatttatggc cgtttaatga agagcgggtt gctcgggcga tttatcaagc
    17761 taatacgccg gtgatttcat ctgtaggaca tgaaacagat accacgatcg ctgatttagt
    17821 agcagatgtt cgggctgcca ctccaacagc agctgcagaa ttagctgttc ctgttttgtc
    17881 agaagagttg atgcgcattc aagaacggca agctagattg cagcaggcgt ttgtacgcca
    17941 gatccagcgc aaacaagaac ggttcgagcg tgctcgacgt tcctatgtat ttcggcagcc
    18001 tgaaagattg tatgaaggtc agaccatcaa attagaccag ttgaaacagc gattatatca
    18061 agctacacag caaatttatc atgcgaaaga aaaacaaacg atcgcattaa gccaccaatt
    18121 ggaacaagtg gctccgcttt atcgagttaa agcagctcgc caagaaactg cttatctaga
    18181 aaaacgctta gaagagaaaa tccaacaata tatgaaacaa cagcagcagc gttttgctca
    18241 agctgttcaa tctcttgatt tgttgagtcc attgaaaatc atgggaagag gctacagcta
    18301 tacgacgtta aatgatcaag tagtaaaaag tgtcaccgag attcatcaag gggatttgct
    18361 gacgatccat tatcaagatg gaatcgttaa aggacaagta caagagatag acaaaggaga
    18421 ataaaaatat ggaaaatcca acttttgaag agtcactgca agaattagaa aaaattgtca
    18481 tgcagttaga acaaggagac gttccattag aatcagctct agattcgttt aaacgaggaa
    18541 tggaattaag caaacattgc caagatacat tagctaaagc agaagagacc ttgacgaaaa
    18601 tgatgacaga atcaaatgaa gaagttgatt ttgacggaaa cgaggaaagc tgagatgaga
    18661 ttaactgact tttcagctat tcatctacct gctgtagaaa aagaaatctt gtctttttta
    18721 gatgagcata caacggatag aggactctat gatgcgatga gttattcagt gaaagcaggc
    18781 gggaaaagaa tacgtccctt attactttta acggccgttg cttcctttga tgagcctatt
    18841 gatgttcccg tctatcaagt agcagctgct ttagaaatgg tgcatacgta ttccttgatc
    18901 cacgatgatt tgcctgcgat ggacaatgat gatcttcgta gaggaaaacc taccaatcat
    18961 aaagtattcg gcgaagcatt agctatattg gccggagatg gtttgcttac aggagcattc
    19021 cagctaatca gtatggcaca cttaggtaat tctcctaagc ttctgctgct tcagcaatta
    19081 gctgtttgtg cgggatcaca aggtatggtt gctgggcaag ctgcagatat cgagggcgaa
    19141 tcgaaaaagc tttctttaga agaattagca tttattcatg aaagaaagac agggcatttg
    19201 attcgttatg ccttgctggc tggtggcata ttagcgaagc aacctgaaga aatcttattg
    19261 ctacttcaac gattagccga acatttaggt ttagcttttc agataagaga tgatctactc
    19321 gatgtgattg gtacaacgaa aacattaggg aaaacagctg gtaaagatga acgtatggaa
    19381 aaaaatactt acccgcgttt attaggccta gaaaagacac gtgaggcgtt ggagattgag
    19441 ctgttatccg ccaacaagat catagacaaa ttagaagagg acgttttttc ttttgatggc
    19501 gggctgatgc gccagatgat caagcaattt gatgtggaga aatgagggaa agaatggaaa
    19561 aagaacgcgt agatgtatta gcggtcaaac aaggattatt tgaaacaaga gaacaagcca
    19621 aacgttcagt gatggctggt ttgatttaca acgaaaaaaa cgagcggttt gataaaccgg
    19681 gagaaaaaat tccagtgagt agtgaattaa gagtcaaagg taaaaaactt ccttatgttt
    19741 ctcgaggggg attaaaatta gaaaaagcac tcaaagcatt tgatctatct gttgaaggaa
    19801 aaacattgtt agatattggt gcatccactg gcggatttac tgatgcagct ttgcaaaatg
    19861 gcgcaaaaat gagttatgct ttagacgttg gctacaatca gctagcttgg aaaataagac
    19921 aagatcctag agtcgtcgta atggaaagag tgaattttcg ttatgcaaaa cccgaagact
    19981 ttaccttagg aattccagaa gttgctgtta tcgatgtttc ttttatttca ctaaaactga
    20041 tgctgcctcc attacatgct attttaaaag aagagggaga agtgattgct ttaatcaaac
    20101 cccaattcga agcaggcaga gaagctgtag ggaaaaacgg aattgttcgt gaccctcaga
    20161 cccatcaaaa agttttagaa gacattttgt catttgcggc acaaggtgga tatgatgtgc
    20221 tggaattaag ttattctccg atcacggggg gagaaggaaa tatcgagttc ttggctcact
    20281 tgaggaaagt acccgaaagc ggaacaatca attcagctat taatatggca gaagtggttt
    20341 caaatgcaca cgaacagttt gaccataaat aaattgtaac tgtgaaaatc aagtgagaag
    20401 tatgcggatt cttctttatt ataccaagga aataagctat cattgattaa gaagaatatg
    20461 ctaaggagcg aagacgtaat gagaaaaaaa gatcggcatc gtttgattac tcgattgttg
    20521 agtgagcaag atatccgaaa gcaggaagaa tttgttgaaa tcctgaaaag caaaggaata
    20581 tctgttactc aagctacgat ttccagagat ataaaggaac tgaaactgat caaggtaccg
    20641 gcttcagatg gcggctaccg ttatagcata ccagctgaaa caaacgaaga tgtaaatact
    20701 aaattggaaa aactattaaa agacgctttc gtggcagttg accaaatgga aaaatttgtt
    20761 attttgaaaa cattgccggg aaatgcttcg gcagcagcaa atttgattga taaacgttac
    20821 aagaaagaat tattttcgat tatcaatgat gacgataatg ttttgatgat tacacgaact
    20881 gaagaccagg cagttttatt gaagaaagac ttcctgcatt acctttaagt tgaggagcga
    20941 atgtgatgct acaagaaatc agtattacta actttgcgat tattccggaa ttgcggcttt
    21001 cttttcacga aggaatgacc gcgctgaccg gagagactgg tgcgggtaaa tccattatta
    21061 tcgatgcttt aggtctgtta gcaggaggaa gaggttcaag cgattatata cgtcaaggag
    21121 cagaaaaatg tgtgcttgaa gggttgtttg aactaccaaa acaagaagga ttctctgaac
    21181 tgatggttga attagggatc gaaacagatg aggacaatct gattgttcga cgggatatgt
    21241 ccttaactgg aaaaaatgtt tgtcgagtga atggtcatat tatcacatta gctaatctga
    21301 gaagaatcgg cagttattta gtagatatcc aaggacaaaa cgaacatcag gaactgctcc
    21361 agcctgaatc acatcttgct ttattggatc gttttggaga tgtagctttc caacagaaga
    21421 aaaagagtta tcagcaagaa tatatctctt atcgtgagct ggaaaaaagg gtacgaaaag
    21481 tgcagcaaaa tgaaaaatcc tatgttcaga gaatagatat gctccatttc cagcaagaag
    21541 aaatagctgc tgctgatttg caagtcggag aagaagagaa attaaaagaa gaaagagaaa
    21601 aacttagcaa ttatcaaaaa attgctgatg gattagctgc aggttacgga gcactgacga
    21661 ataatgaaca aaacagtgta gatggtgttg gcttggccgt ttcagaactg caagggatcg
    21721 cccatctaga tgtggagtat gaagcaattt atgaaaatat ccaaagtgct tactatttgc
    21781 tgcaagatgc aatcggcgat atgagtcgtc agattgattt attggagtta gatgagagtc
    21841 gtttagaaga agttacgcaa cgtttagaat taatccgaca gttgaaacgt aaatacggcg
    21901 aatccattga gtcaatattg gcttactatg acgaaatcac cgaagagcta gcttcttctg
    21961 atttttcaga aggtcaattg gacaaaatga aagaggaact agagcaaaaa gagcttctac
    22021 ttcagcaaca agccgcagat cttcatgaag cgcgtaaaaa aatcgcaaaa gaattggaaa
    22081 aatccattct gcatgaattg aaaagtctgt atatggaaaa cactgagttt gaggtacgtt
    22141 tcttaaaaga agaaaataga caattgaatc atgatggatt tgaccaaatc gaattttaca
    22201 ttacgaccaa tccaggagag ccactgaagc ctttggtgaa agtcgcttct ggtggcgaac
    22261 tgtctcggat gttgcttgct ttgaaaacta ttttttcttc tgagcaaggt gtgacaagta
    22321 ttatttttga tgaagtggat acaggtgtga gcggaagagt tgcacaggcc atcgcagata
    22381 aaatttctca aatatcgaaa tactctcaag ttttgtgcat cactcatctg cctcaagttg
    22441 ctgcagtcgc cgactatcaa tattatattg tgaaagcagt gattgatgga cgtacgcaaa
    22501 cttctgtttc agagttgaaa acaaaagaaa gagaagaaga aattgcacgt atgctggctg
    22561 gaagtgagat tacaaaactc acactcgaac atgcaaaaga actattgaaa ctcgcaaaaa
    22621 ggacctgagc cacctcgcag tggttcaggt ccttttcagc agctgatagc tatattttat
    22681 ttttacatga cactcattac agcagtcaat acaacagatc caatcgttaa aatcagcatt
    22741 gtccaaatga caaattttgt tactttagca aaagtacttg tttgtttttt ttcacgcatt
    22801 gttttcaacc tcgtcattaa ttagttgatt caagtgtaca atcttttttc catttccaca
    22861 agagggcttt tttataaaaa cttccctttt tttaaataat aggtagttac gatacaaata
    22921 aataatgtta atagaactag ccatataaag gcatcatccc tttcagcaat aggtaactta
    22981 atgttcattc catagatact gccgacgatt gtcggtatag ttaaaataat cgttaaagaa
    23041 gttaaaattt tcataatatt attcagatta ttcgatacga ttgctgaaaa agtctgattc
    23101 atttgttcta ctagttttaa ttgaatgcga gaagtagtca ttgcctgctc tgtttccacc
    23161 aatacatcac gtaaatgatt tttcaaagca tgattttctt ggaaaagttt attttgcgct
    23221 agttctttca atgcatctaa attagcagaa gtcgcttctc tgaaataaac gaggcttttt
    23281 tggatatcca tgatttgata aagttgtttg ttttcagtgg atacttgaag tttggcttcc
    23341 aaagtatcgc attgagattg aagcgtagct aaataactgt tgtaagagag tgtgacctgc
    23401 caaatgaatt gaaagaagat attgagatta ggcgaaatat cactttttgg aaaggtctgg
    23461 ctaaagactt tctttaaaaa cagtggttca tgattcgtaa ttgtgataag cttctttttg
    23521 ggcgtcaaaa taatagagag cgggtatgta tccacttgaa aataaccgct aggacttgtt
    23581 ttagcaaaag gatattgtaa caataataac gctggctttg tcaactttga ttgattgaat
    23641 tcttctgtac gggaattttc tgcatcgtcc aatatggaag tgatgtaatc tgacggaatt
    23701 tcaaattgat gtgtcagctg atgaatctct tcttgattag gacgttctac acaataccag
    23761 atcgtttggt cagtttcctt tccggtatta cgcagtttct cattatctaa tgtaaaatat
    23821 ttgatcatga ttttcctcct gttgcctgtt tcttttatta taacggatag ggaattggaa
    23881 tacatggaat gtgtttgtaa gatatagaat ataactgcat aaatttaaat aaaatgaaac
    23941 agttgtgtaa agtatataca gtatacttga agcaacgata aaaaaggagt ttacgttcat
    24001 gggcacattc acaccactta tcttgttttg gtatatttac ccggtaatcg ttttgtttgg
    24061 ttgccaattt cttgtaaaat tattttcgtt aaatcgtcgt tttaaaataa aagcccctga
    24121 tttggcgatt ccttttttgt ggggcggtat gcatgcattg tctagaaata cagggacaat
    24181 ttctatctta ccgtttttct ttatttctgt tttgctaatg ggaatttgta ttgctgtttt
    24241 ccaagcatat tattatgaag aaattattta tccgcgttat ttcaagatga cttggcgttt
    24301 ggtttttctg ttgacaatcg tcttgtacgc tgtcttgatc attgtgaaca tactttctta
    24361 cctatgaaaa aaatagaaaa gctgtaaaaa gtcttttaaa aggctttttg cagcttgatt
    24421 tttaaaaaaa tgtttatgaa taaaaaaagt tgttcccgga tttcccagaa aaggtggtag
    24481 aaagtggggg attgtggtag actaatttta gttagtggag gaatgggggg ctttcaatgt
    24541 tgatgggtga attccaacat aatattgatg caaaaggacg tttaatcgtg ccctcaaaac
    24601 ttcgtgaaga acttggcgaa aagtttgttc tgacacgcgg attggatggc tgtcttttcg
    24661 gctatccgat gagcgaatgg gaaaatctgg aagctaaact gaacgaaatg ccgttggcta
    24721 aaaaagacgc acgtactttt gtgcgattct tctattcggc tgccactgaa tgcgaacttg
    24781 ataaacaagg acgaatcaat atacccagca cgttaagaaa ttatgcagct ttgacaaaag
    24841 gttgcgtagt catcggtgta tctaaccgaa ttgaaatttg ggatgagaca cgatggcaag
    24901 atttttctgc agcagcagaa gagaactttg acgaaattgc tgaaagtatg attgatttcg
    24961 gactataaga ataggaagag gctgactaat gactgaaaca tttcaacact atacagtcat
    25021 gttgaaagaa acggtagatg gattgaacat caaaccggat ggcgtttatg tagactgtac
    25081 actcggcggt gccggacata gtgaatattt attaagccaa ctaagttcta aagggcatct
    25141 gtatgcgttt gaccaagatc aaaaagcaat cgatcacgcc aagctgcgtt tagcttctta
    25201 tgtcgaacaa ggtatggtca catttatcaa agcgaacttc agagaattag aagaacgctt
    25261 gagtgagcat gtagataagg tagacggcat tttgtatgat ctaggggttt cctctcctca
    25321 gctagatgaa gcagaacgtg gatttagtta tcatcaagat gcaccgttgg atatgcgcat
    25381 ggatcagtca gctcctttat ccgcttatca cgtaattaat gaatattcct accatgaatt
    25441 agtaaagatt ttcttccgct atggcgagga gaaattttcc aaacaaattg ctcgtgaaat
    25501 cgaacgagta cgaaaaacaa aaccaatcca aacaactggt gagcttgtcg aaatcattaa
    25561 acaagtcatc ccagctcccg caagaagaaa aggcgggcat cctgcaaaaa gaatttttca
    25621 agctgtgcgg attgcagtaa atgacgaact cggtgccgaa gaggcctctt tagaacaggc
    25681 aatccggtta ttgaaagtca atggaagaat cagcgtaatc acattccatt cattggaaga
    25741 cagaattgtc aaaagtatgt ttaaagaata tagtacggta caagatctac caccgggact
    25801 tcctgtcata cctgacgaat tccaaccaga actgaaagtc attactcgta aaccaatcct
    25861 tccttcagag gaagagctaa cagaaaacaa tcgatcacgc agtgcaaagt tacgtattgc
    25921 agaaaaaata agaatgaaag aggagtagaa tgatggcaga attaaagaaa atgcaagatt
    25981 acgaatatga tttacctgaa ttggtggatc agccggaaca acaaatgcct gattcttctt
    26041 cgaatcaaga aatcattctt ccacaatcac cggcaaaacg attaaagcag atttctgctt
    26101 tggaaaaaat tgctgtggca agcgtggcga ttgctattgt tgctttatgt gttttaacag
    26161 ttatgctgcg cacaaatatc agcggagctg aaaaagaaat cacgtcaata caaacagaaa
    26221 tcaatgataa aaagcaagaa aaaacgagtt tagaacaaga aaaaaacgaa ctttctcgta
    26281 ctgaacggat taaacaaatc gcagaagaaa aaggattgtc tattaatgac gacaatttaa
    26341 ggaaagtgaa gtaaatgagt cttaaaaata aaattcggcg gtttatggaa aagaaaaatc
    26401 taaatccgat gaacaatcgc aaaaaagtag gtatcatttt atttgctacg agtattggat
    26461 tgttcttttt atttgccgtc cgtttctcgt atatcgttat tggaggtcat gttgctggta
    26521 cctcattagc tgaaaaaaca aaacagttgt accaaggaag tgaagtagta aaagcaaaaa
    26581 gaggaacgat ttatgatcgt aacggtgtgg cgctcgcaga agatgcctcc tcttattcaa
    26641 ttaaagcgat actctcaaaa acatatacct ctggggataa aaaactgtat gttgaagaaa
    26701 agaattttga taaaatagca gaaatcctgc ataagaatct ttctatagac aaaaaagatg
    26761 cactgaatat tttagaggac ggcgcaaaaa aagagctgta tcaagttgaa tttggtagct
    26821 atgggaaaaa tatctcccaa gaaaccaagc aaaacatcga agcggacatg aaaaaagaag
    26881 gtgtcgctgg tctttatttt gtcgaccatc aggctcggat gtatccaaat ggcgtctttt
    26941 cttctcattt tattggatac gcagttccag acaaagatga gaatggctta gttggtaaac
    27001 taggtttgga gtctgcttac aatgatatct tgagcggtaa agatggtaag attatttatc
    27061 aaaaagataa ttttcaaaat cctctgccag gtacagtggc agaagaagaa aaagctgtcg
    27121 acggacaaga tatctacacg actttagaca gcagactaca aagttattta gaaacactga
    27181 tggatcaagt aaatgaagaa tatcagccag aagaattgac agcagtcctg atgaaagcaa
    27241 aaaccggaga aatcttggca atgggacaac gacctacttt caatcctgaa acaatggaag
    27301 gattgactgg aaaagatgct atctggcgta atttcttggt acaagatagt tatgaaccag
    27361 gatcaaccat gaaagttttc actactgcag ctgctatcga agaaggagaa ttcaacgaga
    27421 atgaaacctt ccaatctgga aaaatccaag tagaagatgc tacgatcaat gaccatgact
    27481 ttggtgaaaa aggtgtgctg accatgcgtc aagctctttc atggtcaagt aacgtcggga
    27541 tggtcatact ggaacagcga ttaggcggac gatggtataa ctatttacaa aaactgggat
    27601 ttggacaaag tactcattct ggattagatg atgaagtaaa cggtgcttta cctacattaa
    27661 atattgtcga ccgagcaatg agtgcttatg gacaagcggt aggtgtgacg aatttccaaa
    27721 tgatgaaagc tttcacttct attgcgaata acggtacaat gattcaacct cgatatatca
    27781 gcaaagtggt ggatccccaa actggagaag agcgaacaac tcaaacagaa gttctagggc
    27841 aacctttttc aaaagaaaca acagaaaaag tccgtgaata tatgagagat gttgtcgaaa
    27901 gcgaaaacta cggtagtgct tatggtgtat atagtgtacc tggatacaat gtatcggcaa
    27961 aaaacggaac agcccaaatc gcttctgata cagggggata tcaaacagga gatacagcct
    28021 acctgtattc gattgtggag atggttcctt ctgaagatcc agactatgtc ttgtatctaa
    28081 caatgaaaca ccctaagaca tatgatcgaa tggcattggc taaaatcgca aatccattga
    28141 tgaaacgggc aatggatttt aaagaaactg aagaagattc agatacagaa acaaaaacag
    28201 aaaaagtatc cgtagccgat tatcgtaatc ttgaagcgga cgttgcagca gctgatgctc
    28261 aaaaaagtgg actgcaacct gtagtgatcg gtaatggtaa aaaagtacaa aaacaatcta
    28321 ctgccaacgg tgatcagttg atctctggag aaaaactgat cctatatact ggtggtgata
    28381 agctgatgcc tgacgtaaca ggatggtcaa aagcagatat catgaagctg gggaaaatcc
    28441 tcggtattga agttagtttt gatggagatg gttattgtgt caaacaagaa cttgctccat
    28501 atgaaaaaat cactgaagac aaacttagtt ttactttaga agaataagaa gagaaatagg
    28561 agagaaaaga gcatggaatg gacacaagca ttgattccca ttgtcagcag ttgtgcaatg
    28621 acaattgccg caatgccgct atttattggt tattttcaaa tgaaaaagca aggtcaggcg
    28681 attcgtgagg agggaccaaa gtggcacaat agcaaagccg gaactcctac aatgggcggc
    28741 ttagtgtttc tgatcggctc tattttgaca ggaatttggg ttggagcttg gcaaaaacag
    28801 ctaactccta ctcttttcat cttgcttttt gtattagcgt tatacggagt aatcggcttt
    28861 cttgatgatt ttataaaaat attcaagaaa agaaacatgg gactgaactc gaagcagaaa
    28921 ttgattggac agattctagg gggaatcatc ttttatcttg tttaccgttc tgaaggttat
    28981 ccaggtgtat tgaatttctt cggactagat ctacctttag gaatcattta tggtattttc
    29041 gctctcttct ggcttgttgg attttcaaat gcagtaaatc tgacagatgg catcgatgga
    29101 ttagttgctg gcttaggaag tatctctttt gcagcttacg gtctaattgc ttggcatcag
    29161 catcagtatg atgtattaat catttgctta agtgttttag gaggtttact gggtttcttc
    29221 gtctacaaca gaaaacctgc caagattttt atgggagatg tcggttcatt agcgttgggc
    29281 ggacttttag cggcaatttc tataatgcta aaccaagaat ggacattgct tttgattggt
    29341 ttgatctatg ttatggaaac agcaagtgtg atgcttcaag ttacttcctt taagcttaca
    29401 ggaaaacgaa tttttaaaat gtctccaatt catcatcact ttgaaatgtg cggatggtcg
    29461 gagtggaaga ttgatacagt tttttggttg atcagcattg tgacttcgtt gatcacatta
    29521 tggatcgttt ggtaggagat gacgggaaat gaaaaaaata tcaacatatg aaaataagaa
    29581 agtgcttgtc ttaggactcg caaaaagcgg agtaagtgcg gccaaattat tacatgaatt
    29641 gggcgcactt gtcaccgtta atgatggaaa accttttgac gaaaatccgg aagcacaaga
    29701 gctgctttct ttagggataa aagtaataac aggtagtcat ccaatcgaat tgttggatga
    29761 agaattttca ttgatggtca aaaatccagg gattccctac agtcatcctt tagtagcaaa
    29821 agcccaagaa atgggtattc cggtaattac ggaagttgag ctagcttacg aagtagcgga
    29881 atgcccaatt attgggatta caggaacgaa cggaaaaaca acgacaacaa cgatgacagg
    29941 actactactg aatgcaggag cagatcaagg aattgcacgt ttagcgggta atattgggta
    30001 tccagcaagt ggtgtagcgc aagaagcaaa atcagaagat aaaattgtga tggaattgtc
    30061 tagcttccaa ctcatgggaa tcacagattt ccgccctcat atagcggtga ttaccaatat
    30121 ctatgaagca catattgatt atcatggtac aagaaaagaa tatgtcaaag ctaaatggaa
    30181 ccttcaaaaa aacatgacag aaaaagatta tctgatcctt aactggaatc agtcagaact
    30241 acaagaatta gcgcaaagaa caaaagcacg cgtactgcct ttctcgacaa aagaagtgtt
    30301 ggaagacggt gtgtatgctg acgattactc aatatactat aaaaaagaaa aaataatgga
    30361 aatatctgaa cttggcgtac caggtaagca taatgtagaa aatgcactag ctgctatttc
    30421 tgtagcaaaa ttgtatggta tatcaaatga agctatacga gaaacgttgc attttttcca
    30481 tggcgtacca catcgtacgc aatatgtagg agaaatccaa ggcagaaaat tttataatga
    30541 ttcaaaagca accaatattt tagcgactaa aatggctcta agtggatttg aaacgtctaa
    30601 ggtcgtgtta ttggctggcg gattagacag aggaaataca tttgatgaat taattccttc
    30661 attaaaagga atcaaagcaa tggtcgtatt tggccaaaca aaagaaaagt tgatggatgc
    30721 tggaaaaaaa gcaggcatcg agacaatagt aacagccgat tctgtagaac aagcagttcc
    30781 tttagcatta gaaaacagta cagatggtga tgtagtttta ttatcaccag cgaatgccag
    30841 ctgggatcaa tacccaaatt ttgaaactag aggaaacaga ttcatggaag ctgtgaaccg
    30901 attaaaatag aaaaagtaga agcgagcgat aaaaaatgaa aattttggtt acaggcggag
    30961 gaacaggggg acatatttat ccagctcttg cctttgtcaa ttatgtaaag actcaagaac
    31021 cgaatgctga attcatgtat gtgggagcaa aacgaggtct agaaaacaaa atcgtccctg
    31081 atacaggaat gcctttccat accttggaga ttcaaggatt taaacgaaaa atttccatgc
    31141 ataacatcaa gacggtccga ttgtttctta agagtatccg tcaagcaaaa aaaatcctca
    31201 gtgattttca accagatatc gttatcggta ctggaggata tgtttccgga gcggtagtgt
    31261 acgctgcagc caaaatgggt attccaacag ttatccatga acaaaacagt gtaccaggtg
    31321 ttaccaataa atttttaagc cgatatgtgg atcggatcgc tctttcattt aaagatgcgg
    31381 cttccttttt tcctgaaaac aaagcagttc ttgtaggtaa tcctcgagca caagaagtag
    31441 cagatactaa aaaatcagat attttgaaaa catttgattt ggatccagaa aagaaaacag
    31501 tcttgatttt tggcggtagt caaggagcac ttaaaatcaa tcaagcagta tcagtttttt
    31561 taagtgaata tattccttct tcttaccaag ttttgtatgc ttcaggagat agatattatg
    31621 aagagatcct aaataaaata aatcctgaaa tacaaaatat tagtgtccag ccttatatca
    31681 aaaatatggc agaggtcatg gcttccagtg atctgctggt ggggcgtgct ggtgcaactt
    31741 cgatcgcaga attcacagct ctggggttac cggcagtgtt ggttcccagc ccatatgtta
    31801 cgaatgatca tcagactaaa aatgcaatga gtcttgttca cgcaggagca gcaaaaatga
    31861 ttgcagataa tgaactaaca ggtgaatcac taagtcagac agtcaacgaa atcatgggtg
    31921 atgaagaatt gcaaaagcaa atgtgccgag cttctaaaga acaaggaatc cctgatgctt
    31981 caaaacgtct ttatgactta gtaaaacaaa taatcaaaaa ataaaggcgg tggtcttagt
    32041 gagtaagaaa aaagaaccaa aaaaattgtc tcatccgtct tctgatgaaa acaatttgac
    32101 tccttggcaa aaggctaacc gaaaatatct ggcagaacaa gagatgaaaa accaagagcc
    32161 aaaaaaagaa gacataacat tagatacacc cctttcagat tctgaagaag aaaaaacggt
    32221 tcaagaggaa cagcaagaag aaagccagtc cgagaaaaaa atcgaatttt ttgaagagat
    32281 caagcaaaag aaaaacggtg gaccatataa cggttctttt ttgaaccggc tgcccaattt
    32341 aaaaagtcaa cggcacaaag tactatttcg tcggttagct ttgatcatca ctgtattggg
    32401 tataccgctg atttttttga tctattatgt ttctccttat agcaaattac aagcagttgc
    32461 agtatcaggc aataagacag tcaacagcca agaaatcatc tctgatacaa aattgtcatt
    32521 aggagaaaat gtttggggac agtattttca tcgatctact tatatcgatc gccttaaaaa
    32581 agcacagcca agaatcgaaa cagcaaatat acatttcaaa gggatgaatg aattcgattt
    32641 ggatgtaaca gaatataaag agattgcttt gatcgcaaaa aacaatcaat actacccagt
    32701 gattgaaaat ggaacagtat tagatgaaaa agtagcaaat cctacaaaaa atctccctat
    32761 cttggaagag ttcaaagact ctgcaaaaat caaagaatta acaaagcaat acaatcagtt
    32821 atcatcagaa ttacaaaaag caatttctga aatcaagtac acgccaagag caagcaataa
    32881 aaatctgatc caattgaata tgaatgacgg caatcaagtt attgtaaata tcaataatct
    32941 tgcgaaccaa atgaaatatt attcgcaagt agccaaagat atggatgaaa aaggtgtgat
    33001 cgacatggaa gttggtatct tctcgcatcc ttacggtagc actcaagaaa cggaagaatc
    33061 gggccaatcg gaagaaacaa ctgaaagtga agaaaatgcg gatcaaacaa acgaatcaag
    33121 tgaagaggca gaaacgcaaa aagcacaaac acctggatcg tctagcacaa acggatcatc
    33181 tgattcaaat gattcaagtg aaacaagtca aatagaagat aatccaagta caactccgtc
    33241 aagtttaaat gaaaattgat aaaacaactt taaaaagtaa gaatgtactt tctaaaaacc
    33301 agtaatttat ggtagaattg gaagcagtga atatatctat taataagtat attttaaaac
    33361 ggacgctagt tgttcgcttg tatatggaat agtaagtagg agggaatccc attcatggca
    33421 aaaacaggaa tgtatgtagg ccttgatatt ggaacgacat ctgttaaagt tgtcgtggct
    33481 gaatatatcg acagccaaat gaatattatt ggagtaggaa acgcaaaatc agaagggatt
    33541 aaccgaggca ttatcgttga tatcgataaa acggtccaag cgatacaacg agcagtacga
    33601 caagcagaag aaaaagcagg tatccagatc aaaggcgtaa gtgtcggttt gccagcaaac
    33661 atgcttgaag tagaaaattg ccagggaatg attgcagtta acggagattc aaaggaaatc
    33721 accgatgaag atgtcagaaa tgtggcttca gctgcccttg ttcgttcaat acctcctgaa
    33781 cgtcaaatcg tgtcaatatt gccacaagat tttactgtcg atgggttcga gggaatcaaa
    33841 gatcctcgtg gaatgatcgg cgtaagatta gaaatgtacg gtctgttgtt tacaggacct
    33901 aaaacgatca tccataacat acgtaaatgc gtggaaaatg ccggattaat cgtcaacgaa
    33961 atggtgatta ctcctttagc gctgactgaa tcaattcttt ctgacggaga aaaagatttt
    34021 ggtacgatcg tcattgatat gggtggcggc caaacaacaa cggctgttat gcatgataag
    34081 caattgaagt ttaccaatct tgaccaagaa ggtggcgaat ttgtcactaa agatatctct
    34141 attgtattaa atacgtcatt taacaatgca gaagcgttga aaataaacta cggggatgca
    34201 tacccagaac ggacatcacc agatgaagaa tttccagtag atgtgattgg tcagtcagaa
    34261 ccagtgaaag tcgatgaacg ctacttgtca gaaattattt cagctcgaat ggaacagatt
    34321 ttcaacaaag caaaagatgc attggaccaa atcgaagcat tagagttgcc aggtggcgtc
    34381 gtattgacag gcggagctgc tagtcttcct ggtgttgtgg acttggcaca agaaatcttc
    34441 ggcgtaaatg taaaattgta tgttccaaat cacatggggc tgagaaaccc ggtatttaca
    34501 aatgtaataa gtatcgtgga ttattcagca aacttgagtg aagtgtatca attggcaaaa
    34561 attgctgtta cgggtgaaac ctcagcagct cgtcaaatgg tagttgaaca agaaacaaca
    34621 aacacatatg agagctatga agcacctgaa gaaacaatct acgatgagcc ggaaccaaaa
    34681 gagtccggag aaaatgtaaa aaataaaatc aagggtttct ttacaaatat ttttgattaa
    34741 ttcatcctgg gaggaataag atatggaatt ctcaattgat aataatatca atgacggcgc
    34801 agtaatcaaa gtcatcggag tcggtggtgg cggtggaaat gctgtaaacc gaatgattga
    34861 agaaaacgtt aaaggtgttg aattcattac agccaacaca gacgtgcaag cattaaaaaa
    34921 ttcaaaagct gaaacagtta tccaattagg ccccaaatat actcgtggtt taggtgctgg
    34981 ttctcagcca gaagttggac aaaaagcggc agaagaaagt gaacaatcct tacgcgaagc
    35041 tttagacggt gcagatatga ttttcatcac tgcaggaatg ggtggaggaa ccggtacagg
    35101 tgctgcacca atcgttgcag gtatcgcgaa agaattgggc gcattgactg ttggtgtcgt
    35161 gacacgtcca ttcacttttg aaggaccgaa acgtggccgt tttgctgcag aaggtatcgc
    35221 tcgtttgaaa gaaaatgtgg atacattatt aattatctca aataaccgtt tattagaagt
    35281 tgtagataaa aagacaccga tgcttgaagc tttccgtgaa gcagataatg ttttacgaca
    35341 aggtgtacaa ggtatctctg atttgatcac agcgccaggt tacgttaacc ttgactttgc
    35401 tgacgtgaaa acagtgatgg aaaaccaagg tacagcattg atggggatcg gtgtggctag
    35461 tggagaagat cgcgtagtag aagcaacgaa aaaagcaatc tcctctccat tgcttgaaac
    35521 atctattgat ggagctgaac aagtacttct taacatcaca ggcggtttgg atatgacatt
    35581 gttcgaagca caagatgctt cagatatcgt agcaaatgca gcaactggcg atgtaaatat
    35641 cattctaggt acatctatca atgaagaaat gggcgatgaa atccgtgtga cggtcatcgc
    35701 aacaggtatc gatgaatcaa aaaaggaacg caagtctagc cgaccagcta gacaagcaca
    35761 aatgcaatca cctgctcaaa agactgtatt agatatggat caagctaaac caacttcttc
    35821 agaagaagaa aacagttttg gcgattggga tatcagacgt gaacaaaatg ttcgtcctag
    35881 agttgatgat tccaattttg acaatatcga aaaaaaagaa tttgatacat tcaaccgcga
    35941 agaaacaaaa tctcatggcg atgatgaatt aaatacgcca ccgtttttcc gtcgtaaaaa
    36001 ataaaggaga ggaagagcac atgattgctg acaacttgca gaaaatcaag caagaaattc
    36061 aggattcgtg tgctcttgtt catcgtgatc cgaaagaagt tacattagta gctgtaacaa
    36121 aatctgttga atccgctcaa gctgaagaat tggttagagc tggcgttatg aattgtgcag
    36181 aaaaccgagt agataaatta ttagaaaaga aacaagattt atttgatttt cctgaaataa
    36241 agtggcattt aattggtaat ttacagcgca gaaaggtaaa attgattgta aatgaaattg
    36301 attactttca tgcgttggac agtttaagat tagcagaaga aatccagaag cggttagaga
    36361 aagaattatc ctgttttatt gaagtaaatg tttctggtga ggaatcaaaa catggaattt
    36421 caccagatga gcttttaccg tttgtggaat ctttagctgc ctgcacaaaa atcaaggttg
    36481 ttgggttgat gactatggcg cctaaagaag cttctgatga gcaaatccat aaagtgtttg
    36541 gtacgctgaa agaattgcgt gatcttgttc aagcaaaagg ttatgcccat gcaccatgta
    36601 gtgaattaag tatggggatg agtcaagact ttcaaattgc gattgaagaa ggtgcaacat
    36661 ttatccgtgt aggaacagcg ctattcaaag acgaataaag gaggaacaaa aatgccgctt
    36721 ttaaacagag gagcaattgc taattttttc ggattagctg atgaggaaga gtacgaatac
    36781 caagattatc ctgaaacaac aaaaaaggct gtacagccaa ctaaaaaaaa tacaacacag
    36841 aatcaatcaa ctcattctgt atctgctgcg aatactcatg aaaaacaaaa aacggcgact
    36901 tcacgtcctg taacgcatga aagtattcag ccggctgaaa agaaaaaagt aattgaacta
    36961 catcaatcac ctacaaaaac tgtagcgaaa gaaagaacag cttcaagaac ctctacacaa
    37021 cctaaagcaa atcttgctgg aaaaatcact gtaatggaac caagagctta ttctgaagct
    37081 atggcgattg ctaaacgaat cattgctgga gaagcaacct tagtgaattt tcatttagta
    37141 gaagaaaaac aagcgagaag aatcgttgat tttctaacag gaacagttta tgcactagat
    37201 ggagatatca aacgtgttgg agatgagatt tttctttgta caccgactgg gatggagatt
    37261 gacagttcaa ccgctcaatc ttttgcagat gcgaatttgt ttgatctgta agaggaggaa
    37321 ctacgattta tactattgca ctattacttc atttacttca acgggcagcc tatttctata
    37381 gcattatctt gatggtatat gcattgctct cttggtttcc aggcgggtat caatcagggc
    37441 taggacgatt attagctaag atctgcgaac cgtatatcag tttgttcgat cgtctgcctt
    37501 tgaggattgg acccttggat ttcacaattt taggggcaat cctgatcttg aacttagccg
    37561 tacaaggttt agcacagatc gttatatgga taactgtcca tttagtttat tgattgaagc
    37621 aacaaaatct ttattctcag aatagatagg aggggaaaat tttggatgca aatgtatacc
    37681 agcactttcg ggcagatgaa cgtacattta tcgattcggt aggcgattgg attgaacagg
    37741 ttcaaggaca atatgcaccc tatctgaccg aatttcttga cccaagacaa tcctatattt
    37801 tagaaacact gattcgacaa agcagtgatt tgcgtttcat gttttatggc ggatatgaac
    37861 aggcagaaag aaaaagatgt ttgattttcc ccgattatta cgaacctgta gaggaagatt
    37921 ttgaaacagc attgtatgaa atccattatc ctagcaaatt tgccagccta agtcatggga
    37981 aaatccttgg aactttagtg ggaacgggga tcaaacgctc ttactttggc gatatcatct
    38041 cggacggtga tcgctggcaa gtatttattg caaatgaggt ggatcatttt gttgctgcac
    38101 aagtaacaaa gattggaaag gttgctgtac gcttggaacc aatcaaatac acagaaatga
    38161 ttatgccgaa agatagctgg acacaagaaa gaggaacagt tagttccatg cgactagaca
    38221 gtgtgatctc tgagatttat aatatttcca gacagcggtc caagcagctg atcgaagctg
    38281 gtaaagtaaa agtgaactgg acagaaaata caagacctga ttttgtactt gaactattag
    38341 atattatttc agtcagaggt tatggcagag tccagatcca agaacttgag gggaagacga
    38401 aaaaagaaaa gtacagggta ctgttcggtg tattacgtaa ataaaataaa aaggtggttt
    38461 actcatggct ttaactccat tagatatcca aaataagaca ttcccgacaa aaatgcgggg
    38521 atataatcaa gacgaagtag atgatttctt agatttagtt gttcgtgact atgaggaatt
    38581 gactcaaaga aatcgagaat tagaaaaagc agtcaaacat tctgaagaaa aacttgagta
    38641 cttcaatgaa ttaaaagatg ctttgaacca atcaatcatc gtagctcaag atacggctga
    38701 taaagtaaaa acaagtgcaa gcaaggaatc cgaagtgatc gttacttctg cacaaaacaa
    38761 agcagatgag ttagttgcga acgctgaaaa acgtgcacat caattaacaa ctgatgcaga
    38821 agaaaaagca agaaaaatct tgacagatgc aacagaaaaa gcaagacagc ttgcgacaga
    38881 aactgaagat cttaagaaaa aaacacgagt attccatcaa cgtatcagct tgatgcttga
    38941 gtctcaatta gaacaagtga aaagtccgga atgggatgaa attttacaac cattctccag
    39001 ctacgtgact gacagccatg aagtgatcaa agaagtttta gcaaaacagc ttgacaatga
    39061 aaatgatact gatgtaaact cagacacagg agtcatagaa gcacctatga cggcatttga
    39121 tacacaggct attgatatct cgatcgtgcc tggtgaaaat gaaaacgagt agaacagaaa
    39181 agcactttgt tattcttaaa gcgagccgat gagagtggaa gttcggtaag acactgcatg
    39241 gtgaaagatc ctctacttga gacctgcttt tccgaaatta gagtaagaag cagcggctga
    39301 cctcgttatt ggtcacagag agtttgcact agatagatgc aactaaattt gggtggtacc
    39361 acggcatgtt cgtcccttgc ggctgaacat gcttttttgt gtccggaaaa caaataaagg
    39421 agtgtatggt tgaatgaaaa tgaaagaaac cttgcaatta ggaaaaacaa aattccctat
    39481 gcgtggtaat ttgccaaacc gagaaggcga gtggcaaaaa gaatgggaag aaaatcgaat
    39541 ctaccaaaaa agacaagagt tgaacgaagg aaaacctagc tttgttcttc atgatggacc
    39601 tccatatgcc aatggtaata tccatttagg acattcattg aataaaatca gtaaagacat
    39661 tatcattcgt tcaaaatcaa tgtctggcta tcgtgcacca tatgttccag gatgggatac
    39721 acatggattg ccgattgaac aagtattggc taataaaggc gtgaaacgta aagaaatgtc
    39781 catggcagaa tacttggaaa aatgtcgtga atatgcactt tcccaagtag ataagcaacg
    39841 agcagatttc aaacgcctag gagttgcagg agattgggaa catccttatg ttacattaga
    39901 tccttcttat gaagctgcac aagtacgcgt atttggtaaa atggctgaaa aaggctatat
    39961 ttacaaagga ttgaaaccaa tttactggtc tccgtcaagt gagtcttctt tagcggaagc
    40021 tgaaattgaa tataaagatg taaaatctcc atctattttt gtagctttca atgtcaaaga
    40081 cggcaaagat attcttgatg aagatacagc ttttgtcatt tggacgacaa caccatggac
    40141 attgccagca aaccaaggta ttgctgtaaa tccaaaattt acgtatgtat tggtggaagc
    40201 cgatggccgt aagtttgtcg tagcgaaaga tttgctagaa acagtccaat cagaaatcgg
    40261 ttgggaaaat gtaacagttc taaaagaatt tgctggacaa gaaatggaat atatgactgc
    40321 aagtcatcca ttctatgaac gtgaatcact tgtgatttta ggcgatcatg ttactttaga
    40381 cgctggtact ggattagtcc atactgctcc aggacatggg gaagatgact atatcgctgg
    40441 tagcaaatat aaactatctg tagtgtcacc tgtagactca aaaggattgt ttacagaaga
    40501 agcacctgga tttgaaggta tcttttatga caaagcaaat cctatgatca ctgatctatt
    40561 aaaagaaaaa ggagctttgc tgaagctaga tttctttact catagttatc ctcacgattg
    40621 gcggacgaaa aagcctgtga tctatcgggc aactccacaa tggtttgcat ctatcgataa
    40681 attccgtcag aatattttgg atgaagtaga aaaagtagag tgggttatcc catggggtaa
    40741 aacacgtttg tataatatga tccgtgaccg cggcgattgg gtaatttccc gtcagcgtgc
    40801 atggggagta ccacttccga ttttctatgc tgaaaatgga gaagcaatca tcacaccaga
    40861 aacaatcgaa catgtggcaa aattatttgc tgaacatgga tcaattatct ggtttgaaaa
    40921 agaagcgaaa gaacttcttc ctgaaggatt tacccaccca ggttctccta atggcgaatt
    40981 tacaaaagaa aaagatatca tggatgtctg gtttgattct ggttcttctc atgaagctgt
    41041 tttacgtcag cgaccagaat tatcattccc agcagatatg tatttagaag gatcagacca
    41101 atatcgtggc tggttcaact caagtatcac gactagtgtg gctatcaatg gtgtagcacc
    41161 atacaaatca gttatttctc aaggatttgc tttagatggt gaaggccgta agatgagtaa
    41221 atctttagga aacatcattg caccagaaaa agtgatcaaa caatttggtg cagatatcct
    41281 tcgcttatgg gtatctagtg tagattatga agcagatgtt cgtgtttcta tggatatctt
    41341 aagtcaagtg gcagaagtat atcgtaaaat cagaaatacg atgcgtttct tattagcgaa
    41401 tacagaagat ttcgatccta aaaaagatac tgtttcgtac aatgatttac gttctgtaga
    41461 caaatatatg accgttcgat tgaaccaagt aatcaaagaa atccgagaag aagggtacga
    41521 caaatataac ttcatgcata tttaccgtac agttatgaac ttcttgacgg ttgatctatc
    41581 gtcattctac ctggattttg ctaaagatgt agtttatatt gaagcagaag atgattatca
    41641 acgtcgttgc atgcaaactg ttttctatca aacagctgta gctttgacaa aattattgac
    41701 tcctatcatt ccgcatacag cagaagaaat ctggtcattc ctgaaagaag aagaggagta
    41761 cgttcagtta agcgagtttc ctggttatga agagtttgca aaccaagaag aattgatgga
    41821 tacctggact gctttcatgg acttccgtga taatgtctta aaagcattgg aagaagcaag
    41881 aaacagcaaa ttgattggta aatctttgga agcaaaacta actgtttatc caaaacaaca
    41941 agttcgtgaa atgttaaaag ctttggatgc agatattgca caattattga tcgtttcacc
    42001 agactacttt gaagttatgg cagcagatga acaagtgcca gataacgcta tggtatttga
    42061 tgatgttgct attacagtgg aaaaagcaga cggggaaact tgcgatcgct gtcgccaagt
    42121 tcgtaaagat gtaggagtag atgaaaaact tcctcatcta tgcggtcgtt gtgcaaaaat
    42181 cgttgaagac ttctatcctg aagctgtagc tgaaggattc gaagaaaagt aaaagggaaa
    42241 agggggtgtg acatgttctt tgtcacaccc ccttttgtta tcaatcgttt aattttccac
    42301 gttcacgata ccagttatct ggttgccaga tccattcgaa tccgtctttt tcaagtagtt
    42361 cgaaagctgc tttaggtccc attgatccag ctttgtagtt gggaaaatcc ggttgtgttt
    42421 ggtcccaagc ttttcgaatg acatccacga tatgccaaga ttgagcgact tcttcccaat
    42481 gagagaagtt cgttccatca ccattcaacg catctaatag taatttttcg taagcttctg
    42541 ggctattttc aaccatttct gcactatttc taaattccaa tttgattggt tccgtttcaa
    42601 atccttgccc tacttctttc ccattcaagg ataaagaaaa tccttcagtt ggctgaatat
    42661 agatcgttag aacattaggt ggaagtgtcg tatcgtcaca tggttcatcg acagatgttt
    42721 tgaatacatt aacaggaact tgtttgaata cgatgttgat ccgagtccct ttttcagtca
    42781 aacgtttgcc tgtacgtaca tagaaaggaa cacctgacca acggaagtta tcgatagtga
    42841 atttgcctgc gacgaatgtt tcagtctctg attgagcatc cactttgtct tcttcgcgat
    42901 agcccttgaa ttgtacacca tccaattcac cttgagcata ttgcccgcga acgaagtttt
    42961 ctaacacttc ttcttcgtta taaatgcgga tagctttcaa tgctttgatc ttttctgtac
    43021 ggatttcttt ttcggaaaat gctactggcg gttccattgc aagtaatgct acaacctgta
    43081 aaatatgatt ttgcaccatg tctttaagtg cgccactatg atcgtagtag cccccgcgat
    43141 cttctacacc gaggttttcg ccaaaagtaa tttggatatt gtcgatatag cgattgttcc
    43201 attgagcctc aaaaatatta ttggcaaaac ggatagctga gatgttttgg atcatttctt
    43261 ttcctaaata atgatcaatc ctgaaaatat cttgttctgg gaatacttct ctgatttgct
    43321 ggttcaattc aaaagcagac tggtaatcag aaccgaacgg tttttctatg atcaaacgat
    43381 tgaagccatt ttttgtcata atattttgtg atttcaaatg agaaacgatc gttccaaaaa
    43441 attgaggtgc catagccaaa taatagacac ggttaccgtt taattgatag tgtccatcta
    43501 atcggtcaga caattctttt aatgtctgat aatgcgttga atcatttaca tcatgggatt
    43561 gatagtaaaa atggcttgca aaggtagttg cttcttcatc tgaaggattc aatgcacgga
    43621 ttgtttcttt gacaacttca cggtagtgct catcgctcca gggacggcga gcggttccga
    43681 ttacagcaaa atgctcgcct aaatttcctt ttctgtaaag gcggaacaaa gacgggtaca
    43741 atttacgctg ggctaggtca cctgtaccgc cgaatatcgt aaacaataca tttttttcat
    43801 tagacatgtg gatacactcc tttatatttt atcgacaaaa atcgtgctgg ccgccttaaa
    43861 gctgacgggg attttctttc tatcattttg gatagtgatc ggtccttcat aagctgcgat
    43921 gtcaacgatc tcatattctt cgttgatatt caggtcaata gtgactaagt aatccaacaa
    43981 ttctttttca tcaagcactc gtgcgatacg cacttttgta ccgacaggat aggactccaa
    44041 tgtttgtcgg cgttcttcat ggacaacttc ctctttttta ggaatcgatc ctccatgagg
    44101 acaatgttca ggatgattca aatagttttc taaatgatct gcaagatgtt ccgaagtcac
    44161 atgttccaat acttcagcat catcatggac ttcattccag ctatagtcta aatgttcaac
    44221 taaaaaaact tcccataagc gatgtttgcg aatcagactg ctagcttttt gcagtccgct
    44281 tgcagtcaat tgtacacctt gataagggga atgttctaca agctcttctt tcacaagttt
    44341 agaaatcatt tcactgacag atgcagctga gacatctaga ctagatacga tttgtttatt
    44401 gttgactttc gttgtatctc cacctagttc taaaatgatt ttcaaataat cttctcgatt
    44461 tggtgtcata ttcgatcatc ctttcatggt aaaaaaccat cattagtaag tgtaacaaaa
    44521 aaaacgttaa ttgactattt ttctaaataa aaaacaaagg atttttagac gtttttacaa
    44581 caaaaaacga aagatgatag agaagactca ttctttcgtt tataaaggat atatatatac
    44641 attgggaggg atctattttt cagataacaa cttatatgaa gtgtctgcag attcgtcata
    44701 aagagtttgt tcgggataag aaaagataag ataaaaacca tactgatcac gtacgactaa
    44761 acgatattca tctatttcta ccacagatgt gccaagagac tgaccgttga tcaaaaacgt
    44821 gtaatcagat gtaatctgta attgttgttt ttgctggcta tcactaaaga accatgtacc
    44881 ttgatacttt gagagtcctt ctgacttttc cgcttgtttt ttctttttca agtaaataaa
    44941 acttgagaga acggtaatga gtgaaccaag aaaatagatc atttttttta tcaccaaatc
    45001 actcctttat gagaaaataa tcgcatagac atattctttc ttctttattt cattgtaccg
    45061 aataaaagct aaaatttctc taagactttc gactggtttt tttactgttt ttctacaaat
    45121 tatgatagga tacatagtag gaagaaaggt gacataattt gaagtatcag caagtggaaa
    45181 tagctttttt tattgaccgc ccgaatcggt ttatagcaca ttgtatgaaa aaagacggag
    45241 aaataattat tgcgcatgtc aaaaatacag gtagaggaaa agaggtcctg ataccaggcg
    45301 ctgaagtggc tattgtattt gctcctggac ctaaacggaa aaccgcctat gatctgattg
    45361 ctgtaaaaaa gaagggtgac tggtttaata tcgatagtca attgcctaat cggctggcaa
    45421 ttgatggtat tttagacggc acgatccttt taccaggatt aactggtcca tttacggtat
    45481 acaagcgtga agtaacttat cttcactcca aatttgatat ttatttagag acggtggtcg
    45541 gacaaaaagc atttgttgaa gtcaaaggta tgacacttga aaataaagcg attggtgctt
    45601 ttcctgatgc gcccacactc agaggattga aacatatagg agaattaacg tatgccgcac
    45661 aagatggtta tgccgcttat gtcttgttta ttgctcagtt cgaacatctt catctggcaa
    45721 cgatccatga agaaatgcaa ccagcactag ctgatatggt tcgacatgcg cagcaaagcg
    45781 gagttcaaat cctcgcctat aattgtcaag tatcggcaga tgaggtaact gtcaagcagg
    45841 ccattccatt tgatgtagat atcccttttg aagatcctat acaatagaaa gaaggtaaaa
    45901 taatgatcaa cttaggtatt atcggaacaa attggattac tcatcaattt gtccaagcag
    45961 ctttaagcac aaaaaaatat gaattgacag cggtatattc tagacatttg gaaaaagcac
    46021 aggaatttgg ttctcattat gaaggggata tcgaattcgc aacagatttg aagacttttt
    46081 tcaatattac tcatatggac accatttata ttgcttctcc gaatagtctt cactttagtc
    46141 aagctaagca agcaattttg gcaggaaaaa atgtgatagt tgaaaagcca gctttttcga
    46201 cgccaaaaga aatggatgaa atcatccatt tggcaaatga acacaatgtg tatttttttg
    46261 aagcagcaag aaatatccat gaatcaggtt ttaaaaagat tgccgattac ttaccattaa
    46321 aagacgagat aatcggtgca aatttcagct atatgaaata ttcttcgcgc tatgaccaag
    46381 tattagatgg agaagaaccg aatatcttct cgactcattt ctcaggtggt gcattgatgg
    46441 atttaggtgt ttaccttgta tatgcagctg ttgcttggtt tggaatgcca aaagaagtcc
    46501 attatttccc aagaaagatt tctactggtg tagatggtct tggatggggg atcttacgtt
    46561 atgagaattt tgatgtatcg atccagccag gtaaaatcgg tgattcttat ttaccgtcag
    46621 agatttattt tgactcagga acgctagtga tgaatggagt caatgcaatt gaacaagcac
    46681 aatttcatga tcgcgcgcat aaagaaatca ttgatttggc ggtaacagct gcagaaaatc
    46741 caatggcaga tgaagcagag gattttgcca atgtcatggc tcatccgaaa gatccagctt
    46801 ggggattgtt gtatgaagag tggatcgagc tagctagaaa tgtgaaccaa gtgatttatg
    46861 atttaagaaa aaatggcggc atcaaattcg atgcagataa tgaggaagat ttttaatgaa
    46921 cgaatttgta caagtatttc ccgaaaattg gcagaaaaaa tatgaggata aagggtttat
    46981 ctcaccttct cttattcaac gagcagtttt tcagccacta agtaataaac aaagtatagt
    47041 agcggtttct cctacaggtt caggtaaaac actggcttat ctttggcctc tactactaaa
    47101 cgtggaacca ggtgaagcaa gtgcgttggt catttttgct tcttctcaag aactagctat
    47161 tcaagtagca gatgttgcac gtgaatgggg aaaagacaaa gagttgaaag tccaatcttt
    47221 ggtgggcgga gccaacgtca aacgtcagat tgagggtttg aaggaaaaac cagaaatctt
    47281 gattggtaca ccaggaagaa tattggaatt gatgaaagca aaaaaaataa aagcacatca
    47341 agtgaaaaca atggtatttg atgaagcaga ccagctattt gacgctggga acagccaaat
    47401 cattgatcaa attcttcatc aagctccaac agagtatcag ctagcttttt tttcagcgac
    47461 agcagatcgt tcccttgaaa gcattgaaaa aatcactggg aaaacgtttg agacaattga
    47521 cgtgacagca gaggacgatt ctcgaaaagg actgagacat tactttttac gtgtaccgat
    47581 acgaaagaaa gatgactact tgagacgtct cacgcatata gaagattttc atggtctgat
    47641 tttctttaat caactgactg aattaggcgt aatggaagaa aaattgctgt atagaggtgt
    47701 accggtagga agtctggcct ctgatcagaa caagctactc cgaaaagcag cgctagaaca
    47761 attcaaaaag aaaatggtca gagcactttt gaccacagat gtggcggcta gaggtttaga
    47821 tattacaggt cttccttatg tagtgaatgc agaagttccg ttaagtgaag aagcttattt
    47881 gcatcgttcg ggaagaactg gtcgaatggg taatgaagga atagtaatta cactcgtaca
    47941 agataataat ctaagagatt taaaacggct tttacgtcca tcaaatataa cacttgaaga
    48001 gattttttta tacggaggta agctgcaaac tgagcagcca accaaagaag atgcagctcc
    48061 tgcagtaaat aaaaagtatg cgaaaacttt ttctggaaag gagcagaacc cagaatataa
    48121 caaagaaaaa ataagaaaaa aagttaaaaa taaatcaaaa aaagagagaa ataaaggagc
    48181 aaggcgcaaa taagcgcctt tttatgctat gattatgaaa acgggaacaa aattggttgc
    48241 cagaggaggg gttatgtgaa agaacgtcaa gaagagatct tgcagctatt gacagaaaac
    48301 caacaaggat tgacagcaag tgatgtagct gagcgcttaa ctatcgatcg aagcaatgca
    48361 agccgatatt taagtgaact atacaaggcc catcatattg taaaaaccgc tggccgacca
    48421 gtcgtttact ctttgccaac ggaaaagtca aaatctgatg aagtgcatgt cgactcttct
    48481 acgcaagtga cttttgaaac actcgttgga gaaaatgatt ctttaaaagt cagcatacag
    48541 caggcaaaag ctgcgattct ttatccaccg cgggggttgc atacgattat ttttggagaa
    48601 actggtacag ggaagtcgat gtttgctgaa tgtatgtatc attttgcaat cgattcagag
    48661 atgctttctg cagatgcgcc atttgtttct ttcaattgtg cggattatgc tcaaaatccg
    48721 caactgctct ttggacacat ctttgggatc aaaaaagggg cttacacagg agcagctcaa
    48781 gatagtcccg gtctaatagc caaagcagac ggcggtatat tgtttttaga tgagatccac
    48841 cgtttaccgc cggaagggca ggaaatgctg tttactttca ttgataaagg tatttaccgt
    48901 ccgcttggag aaagtgcaca agtacatgaa gcatctgttc agatcattgg ggcgacgact
    48961 gaatcttcag aaagtttctt atctacgttc aatcgacgga ttcctatggc aatcaccttg
    49021 ccgaatcttg cttcacgttc actggatgag cgttatgaaa tcatctctct ttttatcaaa
    49081 caagaagcga atcgtttgaa tcaacgtatc gatatcgaaa aagaagcgat tcttgccttc
    49141 atgctttatg atgcggaagg aaatatcggt caagtgaaac gagacttgaa actagtctgt
    49201 gcaaaatctt ttttacatta ccgtactcat catgagaaaa agctaatcat tcgaaaagaa
    49261 gaactttccc ttcaagtaca aaaaggatta ctgaaaatca aggaaatacc ggaacgtctc
    49321 gaccgattca tggacagcaa aagtcagtat ttgacatttg aaccaggatt tgctgatgtt
    49381 gtctggtcac aagatccaga acgaaacatg caggtctata acgatattga ggaaaaagtc
    49441 acttctcttt ccgaaaccgg tgtggagaac attgatctag agaccctcat ttcaaaagat
    49501 gtggatgctt atttccagac atatgtcaaa gaattgacta aaagttcgat tcccaaagat
    49561 cttttgccag atgaaatctg gcggttaacc aatcaattgt atgatgtggc tgaaaaacgg
    49621 ttagaccgta tttataatga aaaagctcgt tttgcttttg ctttgcatct gcaaagtaca
    49681 ttggatcgtg tgagagaagg tcatatgatc gtccacccag atttgaataa cgtacggaaa
    49741 aattttaaga gggaattcca ggtagcaatc gatctttcaa cgatcattga agaagaagaa
    49801 caagttgaga ttccttttga tgaaatcggc tttattagta tgtttctttc tatcaatcta
    49861 ggagaaagtg agcctgttaa agaaaacaaa gtagatgttc tcatattgat gcacggcaga
    49921 cagactgctt caagcatgtt ggagatggca caagaactat taggaacaaa agtagggaaa
    49981 gcttttaata tgccactggt tatggaagtt caaacaatgt atgaagaagt gttacaatat
    50041 gtcaaaaact atcaagagca tttgacgaat ggcctactct tgttaacaga tatgggatca
    50101 ttaaacacct tcagtgattt gatttatgaa gagactggca ttcggacgaa agctatcacg
    50161 atgacaagta ccatgattgt gatagaagcg atacggatgg caagtgttgg aagaagctta
    50221 gaagatattt atcaaaatat ccagctttcc tttgaatcga tcgtacgtga tcagttcaaa
    50281 gcggaaaagt ctgaaacttt acagcgtaaa aaagccgtaa tcgtgacatg ctttactggt
    50341 gaaggagtag ctgccaaatt atatcagcgt attatgccag tgattaatca aaataaggta
    50401 gaactgatcc agatgcagtt tttagaacgt gaatcattca aaaaacacat cgatgcctta
    50461 ttggaagaat atgagatcaa agcaattgcg ggaacagttg atgttgatta tcaaaatatc
    50521 ccattttttt cagcctatga cgtttttgat gatgaaaaac taaatatttt aaagcgtata
    50581 gctgatgatg agatcccgac agctaaaatt gtcgattcac ttgctggatc attaagcgat
    50641 gtcttttcta ttgatgaact gatccgttca ttgcaaaaga cagttcagca gatccagacc
    50701 gatttgtcga ttatcgtgga acctggagta gatactggaa tcgttatcca tttagccttc
    50761 atgttagacg gattgatcaa acaagaaagc catcggcaat ttgagaaact gtctattttc
    50821 tcaaaaattt atcgtttgga aatggatatc gttcgggcaa acttgatggc actagaacga
    50881 aaatatcagg taaaaattcc agaagatgat atcgcatttc ttacacaaat gtttcttgaa
    50941 aataaaataa aaaaatgaac acactaatca tactgtttac acactaaaca tctgtgtgcg
    51001 cactataaaa aagagctaaa aagcatgaaa acgctatttc agctcttttt tttattggca
    51061 tgcttcttgc gttatataaa agtgtaaaaa aataaaagga gtgttaaaaa tggcaaaaaa
    51121 aacaatcatg ttagtttgtt cagcagggat gagtacgagt ttattggtca caaaaatgca
    51181 aaaagcagca gaagaaaaag ggatggaagc cgatattttt gcggtctctg cctcagatgc
    51241 agataataat ttagaatcta agaatgtcga tgtattatta ttaggtcctc aagtacgttt
    51301 catgaaagct caatttgaac aaaaacttgc gcctaaaggt attccattag atgtcatcaa
    51361 tatgcaagac tatggcatga tgaacggaga aaaagttctt gcacaagcag aaaatctaat
    51421 gaaataaatc aagtatgcag ttgggggtat aacaacgtgg aagatcaaca aaatctagaa
    51481 gccatcatgg ggttaatcat gtttggcggt aatgcaaaga gcgatgcaat ggaagcgatt
    51541 gcggctgcta aaatcggtga ttttgagtta gcggatagga aaatcgctga tgcagaagaa
    51601 tcacttatac aagcgcatca tgcacaaaca gacatgttga cacaagaagc acaaggaaat
    51661 catatgcaag tgacattatt gactgttcac agtcaagatc acttaatgac atctattgcg
    51721 tttactgatt tagcaaaaga aatcatcgaa ctttatcgtc gcgtatacag cgaataaata
    51781 caacttcttt tttgaacgga agaagaaaaa tttttgaacg gattttttat tttgttataa
    51841 ggagaactct ctttatataa aaaacttata ggaggaagta aaaaatgaac ggactatcaa
    51901 gttggatgga gaaatacatc ctgccggttg ccgcaaaaat cggagcgcaa aaacatttag
    51961 ttgctttgcg tgatgctttt atcggcacac tgcccgcaac aatggcggga tcagtcgctg
    52021 taatgatcaa tgcaattatt cgtgatttac cgcaacagtt taacagtacc tatgctgaca
    52081 gtttagcagc agacaaagga atctttgccg tattgaatgt catcatcgga atcaatggtt
    52141 ttgtctggaa cggtacacta gcaatcgccg gattgatctt tgccttttct tgggggtata
    52201 atcttgcccg agcttatgga gtaaatgatc tagctggggg gatcgttggt ttagctacct
    52261 tgattcaagg tatttccttt ggtccagcat tatcagcagc attagatgtg aatgttcctt
    52321 caaatgtcgc agatgcaatc aatagttctg agattggtgc aacgattgca gatggcacac
    52381 tgtcagttgg attatggggt tggctaaaac tagatcattt gaatggaaac gcttatttta
    52441 cagttatgat catgggagct atttcagtca ttattttttg taaattgatg cttgccaaca
    52501 ttacgatcaa aatgcctgat tcagtaccac cagcagtatc taaagcattt gcagccattt
    52561 taccagcgac gatcgcttta tacgtcattg ctattatcaa ttatatcgta ggtcgtgtag
    52621 ctgatgggca actgatcatt gatttggtgc aaaaatatat tgcagagcca ttcctaggat
    52681 tatctcaagg gttgggagca gtattgatcg tcacagtatt tgttcaagta ttctggttct
    52741 ttggtattca tggaccaaat gtattggcac cagtgctaga gggaatctgg ggacaagcac
    52801 aactaatcaa tattgatatt ttccaaaaag gttataacgg aaaaactggt acaccagccg
    52861 tgttagatgc aatcgaagat ggaaaagctt atatgtgggt ccgcggatcg ttcgatgctt
    52921 ttgcatggtt tggcggatca ggcggaacga tcgtcttact gatagctatc ttgattttct
    52981 ctaaacgtgc agattatcta acagtagcta aattatccat tgggcctggt atcttcaata
    53041 tcaacgaacc agtgatgttt ggtctgccaa tcgtattgaa tgcaatcatg tttatcccat
    53101 tcttgattgc tccagtagta gcaacagcaa ttggatggac agctacttat cttggcctag
    53161 tcgctccggt atcgcagcaa gtcacatggg tagtaccgcc gttcttattg tcattcttag
    53221 caacaggagc ggattggcgg gcaccgatcg taacattagt atgtatggtc gtgactttct
    53281 tgatttgggc accatttgtc atctctgcaa acaaaatgga ccctgccgca ggcgaacaac
    53341 aataagatga acgtgaggag cgggacgctt taagtttcgt tcctcataat gaattttcga
    53401 ggaggaactg aaatgattac aattgccagt gcaatgcaac aagatgaaat caaagaattg
    53461 ttagaaggat acaatgaaga aggaatgact tttacattta aagaaaaaca aggaatcaag
    53521 ctgttctttg aaacaagtgc agaagatccg gaagttgcgg ctaaaaaagc aaaggaacta
    53581 atcaaagcac agccttgggg aacagtcctt tattttcaat caacagctgt ttagaaaatg
    53641 gtttaaaaag ataggatggt tgagatgata aaaaacggtt tatttttatg tagtattggt
    53701 tctttgatcg tgttgtatag tttgaatcca cattcgcaaa ggtatgattt gtatagtatg
    53761 gtcacaggtc tttttctaac aggattagga atctatttct tcatgaaggg gaaaaaaaga
    53821 gaagaagcaa aggagaaaaa taactaatgg aaatcacgaa aaaattaaat attccagcat
    53881 cgttttttta cgataaagtt atggactctg tccttttcga tgtacgtaaa gcgaccggca
    53941 aatcagtaac tagaaaacag ctgaaaaatt tggagtacgt caaacaattc tcgcaaaata
    54001 accgggctcg cataaaaatc gaagaagtca ttgaaaacca gtcttataag tttcgtacat
    54061 cgacaacgaa gaatgaattc gtcgttcatt atcaaatcaa accattggac gattcgacct
    54121 gtgaggttca ttacacagaa aaaatggagt cttatggcat gcttcaaaag ttgaatgata
    54181 tgcttttagg aacaatcttg atgtatttta aacggagacg tttcaaaaaa atgctagata
    54241 tgatcgaagc aacgtattaa atcaatgaaa catggtacta tttagttatt gaaaaatgaa
    54301 gtaagctaga aggaattttt cataggaaga tagatctttc ctgcgaatcc tcactagctt
    54361 ttttgtagga aggggcagca aaatgacaaa caaagtaaga gagctaggtg tatcttcgat
    54421 tgaggaaatg tttgatttag ccacttatgc atttaatggg gcagatacac ctgaaaggcg
    54481 agaacgtttt cgtacacttg cggagaattc ttggaattac ggattttttg atgaggagga
    54541 tcggctaaca agtcaagtca tggcgacccc ttttccagtg aatttttacg gacaagagta
    54601 cctaatggcc ggcattggct atgttgcttc ttatccagaa gcaaggggac aaggcgggat
    54661 caatcagatc atggaaaaga tccttgaaga ttgccgtgat cgtaaagtaa gcttatctta
    54721 tctggcgcca ttctcctatc ccttctatcg gcgctatggc tatgaacaat catttgataa
    54781 aatcagttat caactaaaca gtcgtgatgt gccgtttatc aaaaaaaaat ctggaaaaat
    54841 aaagcgtatg gattttgaag atgcaaaagc tgcaatcaga agaatttatg aacagatgcc
    54901 ggaaaaccaa cgagctggtc tgaagcgcga agactggtgg tatacgtata aattcaagca
    54961 aaaaggcaat taccagtttg ctttgcattt tgatgataat gaggaagcaa cgggatatat
    55021 cgtttatcag cttgcaacgt cttcctttat tattgcggag tggggatttt tggatcatga
    55081 tgctttttgc agccttgttc gcttcgtaag ttctcacaat ggtgcttttg aaacatttga
    55141 atattcatgc ggctacggcg gtaatgatca aaattatcta ttagaaaatc catttgcttc
    55201 tgtaatgatc acaccttata tgatggcgag gatcgtagat atttctttgt ttttggaaaa
    55261 ttatccattt aaaaagagac aagctgcatt tgccctacaa atagaagaag atcattatgc
    55321 gaaatggaat gaaggaatcg tggaagtaac aatcgaaaat ggaaaaaatc atctctcaaa
    55381 agtagaacaa acaaaactcc caatcgttcg aggaagtatc caacatatca cgcagctatt
    55441 attagggtat cgtaaagtgg aagagttggt attccaagaa agaatacagg tgacaaaaga
    55501 atatgaagaa acgttaagct caatcttgcc acagcaacag ccaattctta atgactattt
    55561 ttgataaaaa acgggtgatg acaatatttt tgtcaccacc cttttgtttt tcatttggca
    55621 tttgaaggaa aatgttaaag taaatcctct tttttcgtaa atttgaattt tttgttttct
    55681 tttgttttag ggttttcttt tttgtggtct tggattgctt ctttcgtaat cttttcccga
    55741 ttttttttag atgaatctaa gcgttctgcg tatacttttt cttttggctt atcattcatt
    55801 ggaaaggacc tccctacatt atttcattct acctctagtt tatcatctgt taaggactag
    55861 tggaaacgtt ttgcctaact tataagctga tttttttagc agataagctt gcaagtaaaa
    55921 aacaaacaag ctacaattga tttattgaaa ttgttcctgt agctcagtag gatagagcaa
    55981 tcgcctccta agcgataggt cgctggttca attccagtca ggaacgtaca aataacccct
    56041 gtatcctttg cggtacaggg ggttattttg tataagtaga cattgtagtt tctaaactgc
    56101 tatgtcctaa acgtttcgat acgctaagta tatttactcc ttgataaagt aaaatagatg
    56161 catgcgtatg tcttagtcca tcaataaaca gtcgaagaga aaatagggaa aaattgatta
    56221 ctaaagaaaa acttactagg tcgacggtgt agttgatttt catacttcaa tagattcgaa
    56281 ttaaacgatt tcgattggct tataccagtc ctatccaacg aaagatatat cgttcttaac
    56341 tctagcggta taacttgaag ctttacgtaa agccaatggg aatgaactgg agatgacttt
    56401 ttattatttt tagtaatagc tgatgggtta atacttacaa ctttcctttt caaagtacca
    56461 tataaaaaaa cggtggggtt gtttagcgaa ataaaggatc taagccaata gcttagatcc
    56521 tttattctat gctttatatt attttttgca tgtgtgcgaa agtatactca agcccaatcg
    56581 ccatttcgga aaatcggtac tgtagagcca tcttcacgga ttccgtcgat atccatcttg
    56641 tcagatccta ccatgaaatc tacgtgggtt tgacttctat tcaatccagc ttctgctaat
    56701 tcttcatcgg acatttctgt accgcctttc acactgaagg cataagcaga acctagtgct
    56761 aagtgattcg atgcgttttc atcaaataat gtattgaaaa agatgatgcc agattgagaa
    56821 ataggtgaag ggtcaggtac aagtgcgact tctcctaaac gacgcgcacc ttcatctgta
    56881 tcaagcagtt ttgctaagac atcttctcct tgttcggcag aaaaatcaac aacttttcca
    56941 tctttaaaag taaatttcat acccgagatg atggtgccag cataactcag tggctttgta
    57001 cttgaaatgt aaccatctac tcgatgactg tcaggtgctg tgaacacttc ttccgtcggc
    57061 atatttgcca tgaatttttc tccacgagca ttatagcttc cagcgccttc ccaaagatgg
    57121 tttttaggca atccgataat gatatctgta ccgggagcag tatagtgaag ggcagaaaat
    57181 tgttctcgat tcaattcttc tgcttttttt gctaattttt catcatgctt cttccatgct
    57241 aagacagggt cttcttcata gacacgagtc gttttgaaaa tctgatccca taatgcctca
    57301 acttgttttt cttctggcag ttctgggaag acttttgctg cccattgttt accagccgca
    57361 gccacgacag tccaactgac tttatttgct tgagttgcct tgcgtaaatt cattagtgct
    57421 ttgccattcg ctgattgata agacgctact cgatggctat ctacaccagc aaatgcatcg
    57481 ggatcggcag aaacaacgct gatcctgctt gctccttttt caagccattc atccgcttga
    57541 tcgattttag attgtggtac attctcaata cgatccgttg cagcatgtaa aagaaattct
    57601 ctttggatct ggtcgtcagt ccattgaacg ataacctcag cggctcctag tttataagct
    57661 tcttgtgtga tcaatcgagc aaggggtgcc tgttccacgc tgatctgtaa aacaactgtg
    57721 tgtccttttt ctgtggctac gccagtttct gcaattaagc gtgcatattt ttttagaagt
    57781 tcattaaaat cagataacat aataaaaatt cctcctcacc attttttctg gtaactatta
    57841 gcatgatacc atttaattat gattcaaaca atgaggcttt aaataaaaaa aggaggttat
    57901 ttttttaagg ttaagtttca tgatatatgt tactacttgc tatttcagct tgtgaagaaa
    57961 atcaaaaact aattttgcat atcaaaaaaa catgttacaa tgaaattaat caatggtgga
    58021 agcgttataa aaaaattaag gagattaaaa aatatggcaa gaaaaaagac gattactaga
    58081 gaacagattt tggcagctgc ttatgaagta gctgccaccg atggatttac acgatttact
    58141 gcgagaaaca tcgcgaacaa aatgaattgt tccacgcaac cgatttatct tgagtttaaa
    58201 aatatggatg acttgagaca agctcttttt gagcagatct atgatcattt acaatatgaa
    58261 gtgttcactg ttgagcatac agggaatacg attatcgatc ttgcttttaa ttatatccat
    58321 tttgcaagaa gagagaagaa gttgtatcgt tcactgtact tagaagaata tggtggagga
    58381 aaagagatgc agaatttctc ttatacttac tttgtgaata cagtgaaacg cgatccagct
    58441 tatgtgaatc tttcagaaga gcagatcgaa tctttgtata atgggacatg gatcatagct
    58501 actgggatcg cagctttgat gtcttcaagc atcatccatc caactgatga acagattgaa
    58561 aagatgatcc aagacagtat agatgctatc ttgaaattca aagaaccaat cgaaatcgat
    58621 tgaatatact caaaagttcc acaaatcagt aggtaacaag agatgtgtca aaataaggaa
    58681 aagttcgttt ttcttacagc gtatgcattt ttaactcccg aagaactctt ccatgaatgc
    58741 aatgaataaa agagccatac agattgaata aatctggatg gctcttttat tgcaagtata
    58801 ttaaattttt gcgactagtt ctttagcgaa agcttctaaa ttttcgatat cttcttcttc
    58861 agcattcaga tccactttaa cattttcggc tccttttgat gcgcctgttt ttgcaaaagc
    58921 agcatcgaaa tcatccaccg atttgcagaa ttcatcgtaa aaagtatcac cagatccgca
    58981 gacaccgtaa atttttccca atagatccaa ttcttgcaag tcttcataaa agtctacgat
    59041 ctcatcaggc agatcgccat catcgtatgt ataagtagct actacgcaaa tatctgcctc
    59101 ctcgaattca tctgcatcta cttgggtaca ttcgttgatt tcgacttcga tatccagatt
    59161 ttcaaatgct tctgcaacaa tatcggcgat ttcttcagtg tttcctgtca tgctagcata
    59221 tacgattttt gctaaagcca tgatgtgcct cctcgtttat aattatttaa caattatgaa
    59281 ttataccaaa acatcattga aaagaaaata aaacctaaag acacctgggg aatgtcattt
    59341 ggctaaatgt atgccgctat gttaaaatga gagggatttg taaaaaggag acgaaaatat
    59401 gattacttta aaatcagcac gtgaaattga aatgatggca gaatctggag ctttattagc
    59461 agatgtacat aaaaatctgc gtgattttat aaaaccagga atcacaagtt gggatattga
    59521 ggtatttgtc agaaactata tcgagagtca tggcggaatt gctgcgcaaa ttggctttga
    59581 agggtatgaa tatgcgactt gtatcagtat aaatgatgaa atttgtcatg gattccctcg
    59641 taaaaagcct ttgaaaaacg gtgatttggt aaaagtcgat atgtgtatcg atttgaaagg
    59701 cggcatttct gattcttgtt gggcctatgt agttggagaa tctacacctg agatcgaccg
    59761 attgatggaa gtaacgaaga aagctttgta catcggtatt gagcaagcac aagtagggaa
    59821 tcggattggt gacatcggtc atgctatcca aacttatgta gaaggcgaaa atctagcgat
    59881 cgttcgtgat tttattggtc atggcgttgg tcctacgatt catgaagaac cagcagttcc
    59941 tcattacgga gaagctggaa aagggttgcg gttgaaagaa ggcatggtta tcacgatcga
    60001 acctatggtt aataccggaa cttggaaaat gaaaatggat cctaacggct ggactgcgta
    60061 tacaagagac ggcggacttt cttgccaata tgaacataca ttggctatta ctaaagatgg
    60121 tccaagaatt ttgacatcac aaggagaaga aggcacttat tgatctatcc agtcaatagg
    60181 tacgggagag gtacatgaaa gtttggaaca aaataaaaca aaatgaaaat ctgatgcgct
    60241 ttattgagac gactcaaaag cgcatcactg attcagaaat gagtacgacc tctgtcgtcg
    60301 ttgcttatta tttactcttg tctttatttc cattgattat tgcttttggt aatattttac
    60361 cgtttctcca catcgatcaa gagactgtac tgacctattt acgagaagtg attcctgaga
    60421 ccgtttacca gtttatcggt cctgcaatca aagacctttt gacacaaagt tcaggaggct
    60481 tgttatccgt ttctgcatta gcggctttat ggtctgcaag tcaaagtatc aatgctttgc
    60541 aaattgcgat gaataaagcg tatggcgtag aaaatagaaa aaattttttg atcgtccgtt
    60601 tcttctcact aattgtcatt gtcttgttca tgattgctat tagtggggta actatcgttc
    60661 ttgggttggg acagctgatt ttggatgcta ttcagccaat cgtacaaata cccgtgaact
    60721 ttatcgatca gttccaaact ttgaagtggc caatcacatt ggtagcttta tttgtcatca
    60781 tgttcttgat ttatttaatt gtccctaatg ctcagttgaa gttgaagacg atcattccgg
    60841 gcacgatctt cgctactgtc ggctggatgc tgttatcaca agtgtttagt atctatgcta
    60901 ggtattttgc aacgaggatc agcggttatc agatcatagg aagttttatt gtactcatgc
    60961 tttggcttaa ttttgctgct acgatcatca ttttgggcgg aatcatcaat gcagttgttc
    61021 aagaatacgt gacgggtcat caaatcaaaa aacgtcgaga tccaagccga aaatggttca
    61081 ataaattgaa aactaaattt tctcataaaa aaagtagaaa caagagttct tattgtaaat
    61141 ttatatcagt aatttataat aattatgact cttaattaaa gtaggtgccc atatgcttgt
    61201 ttcttttagt atggttataa gattttttct gacaatttta ttatcactga tattaacacc
    61261 tgtattcaaa atcatttcag tacaaacagg tatggtcgac aaaccaaatg aaagaagaat
    61321 aaataaagtt ccaatgcctt cagcgggcgg actgcctatc tttatttcat ttgttatatc
    61381 gactttattc ttattccgta aaattatccc tcaatcgtat attgtaccga tcttgattgc
    61441 ttctttagta atcatagtca caggagtgct cgatgataaa tatgagctga cacctaaaca
    61501 aaaaagtata gggatcttga ttgcttctct tattatttat tttgtagcag atatacgtat
    61561 tgattccttc acgcttcctt ttattggata tatccaacta ggctggctta gttttcccgt
    61621 aacgattttt tggatcttcg gtatcacgaa tgcggtcaat ctgattgacg gtttggatgg
    61681 gctggcggca gggatttcat tgatcggttt aattacgata ggaatcattg gatatttttt
    61741 cctccatgct tctacagtgt atattccagt tgttattttc tgtcttgtag caagtattat
    61801 cggttttttt ccttacaatt tttatccggc aaagatttat ttaggcgata caggagctct
    61861 attcctaggg tttatgatgg ccgtcctttc actacaaggg ctgaagaacg tgacttttgt
    61921 ttcatcgatt tctttgctga tcgtcatggg agtgccggta acagacacgt tttttgctat
    61981 cattaggaga aaagcaaacc gtgtttcttt ttctacagca gataaaaaac atcttcatca
    62041 ccgactaatc agtttaggct ttactcataa aggtgctgtc ctgacgattt atgcgatggc
    62101 tttgatgttt tctttcacag ctatggtcat gaattatact ggaagaatcg gaacgatcgt
    62161 attgattatt gccatgttgt ttgctgcaat tttattattt gaattgattg gtctgatcaa
    62221 tgagaaacat cagtttattt taaaaacatt gcgattttta gggaataaag agtaccgtac
    62281 gcaagtaaaa cagaaatatt tgaagaaatg gaaccgtcat atagatgaat aacaaattaa
    62341 accagccgat ccttattagt attgtgacgg caaacagtga taaaattttt cggacactag
    62401 ataattttat ttcaactgta aaagatgaaa cagcagtaaa aatccgaatt tttgacaaca
    62461 attcacttcc cgagtacaaa aaaagactaa cagagtatag gaaatatccc tacatcgata
    62521 tctattttca tgatgaaaat tccggatttg gttatggaca taatcacaat ctgatagcta
    62581 gcgattctcg ctatggcatt atttgtaacc cagatatttt agtggatgaa gagacagtta
    62641 cagcattggt cttgttattg aaagaacatc cggaatgtgc gatgttagca cctaagattc
    62701 ttaatgaaga tggaacaact cagcacctca tccgtgagca gctggctgtt tttgattacg
    62761 ttttgcgctt tgtgcctttc aaatgggtca aaaaaaagtt tgacaaacga ctggctgtgt
    62821 ttgaatgccg aggattgcct actgatgcta atagttatgt acggatgatc tcaggtagtt
    62881 ttatggtagc tgatattgat aaatttaaag agataaaggg gtttgatgag cgtttcttca
    62941 tgtactttga agacaacgac ttgtgtttga gttttgaaaa agcaggttac aagttattgt
    63001 acacaccctt ttatgcagtt atccattttt acgggaaggg tgcgcatcga aatgcaaaac
    63061 tgtttcgcgt gtttttgact tctatgatga aatttttcaa taagtggggg tggcgttttt
    63121 tctgatgaaa ccagaaatat tagtagtcgt tgtgctttat cagcaactat tcagtcaatc
    63181 tccagcttat ggaccattaa gcaaagcact tagcgaaaag caaattcagc ttattattta
    63241 cgataatagt cctcaaaggc agcaaaatga gctgtttgaa aaagaaaata tcgtttatca
    63301 tcatgatccg caaaatcctg gtttagcggt ggcttataat tttgcgttgt ctcaagcaga
    63361 tgaaggaaca cgttgtctcg tcactctgga tcaggatacg aggctagtcg acgattattt
    63421 tgaactattg cgtaaagtaa cctttacaga tgaatgtgtt gctgcagttc cgatgatttt
    63481 ttctgggagc cgacaagttt ctcccctata ttcagatcgt tatatcaatc gtacagctca
    63541 agcagtagaa gttgaaggcc caacagctga acgaatcatg gcaatcaatt caggtgttgc
    63601 ctggtctgtc aaatttctaa aaaagattgg cgggtttaat cctgcttttt ctttagattt
    63661 tttggatcat tggctgtttt ggaaagtgga tcagttggaa aaaactgtgg agatccttcc
    63721 tgtacgaatg gaacatgatc tttcggtttt agactatgaa aaagtttcaa gtaatcgata
    63781 ccgttctatt ttacaagcag aaaatcgatt ttatcatgaa tatgatcaaa ggcatttatc
    63841 cagtcaccga cgacaattat tgttaagaac agctaaacag ttcttgaccg tcaaaaaccg
    63901 gcatatctgg cggatgactt tgcgctcata tgttaagaat tggaaggtgt gaattcgtgc
    63961 gttcggtttg tatagctacc tttaatggag gcagatattt aagagagcag ttagatagta
    64021 ttttatctca gctttcagca gaagatgaag tagtcatttc tgatgatggg tcagtagatg
    64081 atacatggga tatattaact gaatacgcag aaaaagataa gcgaatccag cttttttcgg
    64141 ggcctcgtca aggattgatc gctaattttg gttatgcgat ccagcagacg aaaggagatc
    64201 taatcttttt ggcagaccaa gatgatgttt ggttggagac aaaagtgaaa aagatcactg
    64261 actattttga gcagaatccg gatcagctag tagtagtttc tgatttaatc attgtggaca
    64321 gcgagctgca agagatccat gattcgtatt tttcgtttcg aaattccaaa gaaggatggc
    64381 ttaataatgt gataagaagt cactatattg gtgctggtat ggctttccgg tcttctttaa
    64441 aacccgatat tttgccgatc ccgtcagaag tgccgatgca tgatatgtgg attggtttgc
    64501 tgggaggaaa gaagactggg ttcctaagag aaccgttaac tctgtaccga agacatgatt
    64561 taaacgcgag tgagattgaa acacattcta aattacggca aaaaatttct tggcgtgttc
    64621 atctgattac ggcgttgatc aaacgaaaat ggtttaaacg ttgataaaag atgtttaaat
    64681 ccttaatatt tccatgacaa atataacaat tgatgagata ataacaaagt gaaatttgaa
    64741 atcaaaaagg agagcagata tccatgaagg gaatcattct tgctgggggc agcggaacac
    64801 gtctgtaccc tttaacaaag gcaacatcga aacaattgat gccgatttat gacaaaccaa
    64861 tgatttatta tccaatgtct actctgatgt tggcgggaat caaagaaatt ctgattattt
    64921 caacgccaca agatacacca cgtttcaagg aattatttgg aaacggaaat gatttaggac
    64981 tgcagatcga atatgcagta caagaaagtc cagatggatt ggcacaagcg tttatcattg
    65041 gagaagaatt tattggagat gatagtgtct gcttggtatt aggggacaat atttactatg
    65101 gtggcggtct gtctaaaatg ttgcaacgag cagcttcaaa agatacaggc gcgacagtat
    65161 ttggctatca tgtcaatgat ccagaacgct ttggtgtagt agaatttgat gagaatatgc
    65221 aagctttgtc gattgaagaa aagcctgctc agccaaaatc aaattacgct gtaactggcc
    65281 tttattttta tgacaatgag gtaatttcta tcgctaaagg tatcaaacct tcagaacgtg
    65341 gagaactaga gattacggat gtcaataaag catatttgga taaaggaaaa ctatccgtag
    65401 aacttatggg acgcggattt gcttggcttg atacaggaac acatgaatca ttgcttgaag
    65461 catctacctt tatcgaaaca attgaaaaac gtcagaactt aaaagtggct tgtttggaag
    65521 aaattgctta ccgtatgggc tacatcgata aggaacaatt gctggccctt gcacagccat
    65581 tgaaaaaaaa tcaatacggt caatatttat tgaatttggc cgctgaataa ggagtcctta
    65641 cagatgaaag taacagatac aaaattaaca gatgtcaaaa tcgtagaaat ggacgtcttt
    65701 ggcgatcatc gcggtttctt cacagaaagt tattcaaaag caaaatttgc agaacatgga
    65761 ttgaaatatg actttgttca agacaaccat tcactatcag ctgaagctgg tgtccttcgg
    65821 gggcttcatt tccaaaaagg agaagctgca cagacaaaat tgattcgggt agtcactgga
    65881 gcagttttag atgtagtagt ggatatccgc aaaggtagcc cgacttatgg tcaatgggac
    65941 ggatttatct tatctgaaca taatcatcga cagttgcttg tacctaaagg atatgcccat
    66001 ggattcgtta ctttgacacc aaatgtgaac ttcctttata agtgtgataa ttactataat
    66061 gcagaagcag atgggggcat cgcatttgat gatccagatt tagcgattga ttggccaatt
    66121 gatccatcaa gagcgattat gtcggaaaaa gatcaccgtc atccgacatt aaaagaattt
    66181 gaagcagaaa atccatttgt ttacggagaa atctaagaat taaggagaaa ttttcttatg
    66241 aaaaacatca ttgttaccgg aggtgccgga ttcatcggct ctaatttcgt tcattacgtg
    66301 gtaaacaatc atccagaagt gcatgtcact gttttggata aactgactta tgcaggaaac
    66361 aaagagaact tggccggact tcctagcgat cgcgtagaat tggtcgtagg agatgttgca
    66421 gatgcagaat tagtggaccg tctagtaaaa gaagcggacg cagttgttca ttacgcggca
    66481 gaatcccata acgataattc attaaaagat cctttcccat ttgtacaaac gaatatcatt
    66541 ggtacgtata cacttttaga agcttgccgc aagtatgatg tacgctatca tcacgtatct
    66601 actgacgagg tttacggcga tcttccatta cgtgaagacc tgccaggaca tggggaaggc
    66661 gaaggagaaa aattcactgc ggaaacacca tataatcctt caagtccata ttcttctaca
    66721 aaagctggtt cagatctgtt agtaaaagca tgggtgcgtt cttttggctt acgtgcaacc
    66781 atttcaaact gttcaaacaa ctatggtcct tatcagcata ttgaaaaatt tattcctcgc
    66841 caagtaacga gtatcttaag cggtattcgt ccaaaattat atggcgaagg aaagaatgtt
    66901 cgtgactgga tccatacgaa tgatcattct tcagcagtgt ggctgatttt gacgaaaggc
    66961 agaattggcg aaacctatct gatcggtgca gacggtgaag aagataataa gaccgtgatg
    67021 gaattgatct tagaaatgat gggccaacca aaagacgcat acgaccatgt aaacgaccgc
    67081 gcagggcacg atttgcgtta tgcaatcgat tcgacaaaat tgcgagaaga gctaggttgg
    67141 cagcctgagt ttacgaactt ccgtgatggg ttggctgaca cgatcaaatg gtacgaagaa
    67201 catgaagact ggtggaaaaa agaaaaagag gccgttgaag cagcatatgc caaaaatgga
    67261 caataataca gaggaaattt cactgtatta atggaaaaac acatgtgagg caggcgctcg
    67321 cctgtctctt ttttaaaata aaaatcaagg agcggataac catatgtttt tattaactgg
    67381 aggaaatgga caattaggta cagaattgcg ccatttatta gatgaaaaaa atctgccata
    67441 tgtttcaaca gatgcaaaag acttggatat cacagatgct gagaaaacaa tggcttatat
    67501 tacagaatta aaacccgatg ttattttcca ttgcgcagct tacacagcag tagataaagc
    67561 agaagaagaa gcaaaagaat tagatgagaa aatcaatgtc gatggcacaa gaaatgtagc
    67621 acttgcagca aaagctgtga atgcacaatt cgtctatatc agtacagact atgttttcga
    67681 tggaaaaaac aaaaatgtag aatacgaagt agacgaccaa acgaatccac taaatgaata
    67741 tggtcgtacg aaattattag gcgaaaaagc agtagaagag attttagaag actattatat
    67801 catacgaact tcttgggttt tcgggatcta tggacacaat ttcgtcttca cgatgcaacg
    67861 cctagcggaa acccgcgaca aactgactgt agtaaatgac cagtttgggc gaccaacttg
    67921 gacacgtact ttggcagaat ttatggtata tgtcatcgaa gaaaaagcac catttggtat
    67981 ttatcatttg tcgaacgaaa atagctgttc ttggtatgag ttcgcaaaag aaatattgaa
    68041 agatacagat gtagaagttg cacctgttac gtcagaagaa tttccgcaaa aagcaacacg
    68101 tccgcagtat tcagtcatga gcctgaaaaa gacagaagcg ttaggttttg tcataccaac
    68161 ttggcaagaa gcacttgcgc aaatgctgga agctgccaaa aaaatgaata agtaatttcc
    68221 tttttatcaa atagaatttg gagcatgata tggaaaaaca aacaagaagt gcacgacgaa
    68281 aagaaaagcc aaaagcgaat caggcagatt cgaatttggt gcttgacctg tttctaaaac
    68341 tccgctatct tattggcgtt ttagtcatcg tggtcatcgt tacttttaat ttaaacggaa
    68401 gttcacttgg tgtttgggat aaatatgtgt cgcaaagaga cgatgggaaa aaaactgatg
    68461 tgatttttgg acagaatcgg gaagtgcggt cagacgaatg gctggtgcag acaccttttt
    68521 acctctcaca agctgaagag gacttttcgt tggtgaacga ggattattca ttaaacggac
    68581 aaaacatgat cattgcgtat aattctcctg taaaggatat tacggttatc ggaaaaccat
    68641 tcaactgggg attctttttc ctcggaagag atcgaggact ttccttttat tgggcgatga
    68701 aattagtcgt catggtcttg cttggattcg aagtactgat gatattaacg aagaggaaaa
    68761 aagctctatc gctaatcggt gcatttgcgc tgacattttc tccagcagtc cagtggtggt
    68821 tcatgcaaca tgtgggcgat ttgattttct ttactttggg actgatgatt gctttttatc
    68881 attatttcta caatcatgat aaaaaatggc tacgaacgtt gatgatgctt ttggtggtta
    68941 ttttcggtat tgggtttatt ttagtgattt acccagccca tcaagtgatg ctggcgtact
    69001 tgctgatttt ttattttgtt ggcctgctta tttactatag caagaaagtt tcatgggatc
    69061 tgttggatgt agtgttgatt ggaggggcag ttttatttat tggcggagtc atggttcatt
    69121 tctggctaac gtcaaaagac gcattgttag cttctttgaa taccttatat ccaggaaaac
    69181 gggtaagtac tggaggaaag tggacgattg gcgaattttt ttctttcctt acaaattgga
    69241 aaattccttt tgaagatatc aactattcga ataactctga agtggccctg ttctatcact
    69301 tttttccaac tgtattccta gcaagtccat ttgtgttatt tggcaaaaaa gatagcgaac
    69361 aaaagttact gggacgtatt ttgatgattt tttgtctgtt ttctatcttt tggattaccg
    69421 ttgggttgcc aaaatggcta gcagaaatca ctctgctgtc ttatgttcct ccattaagag
    69481 ccatccttac ttttagtttt gcggcttgtt tattatcgat ctggtttatc gcttatattt
    69541 ggagagaaga tgttattcct atttggtata aattcatttt gttggcagta aatgcatggt
    69601 cttatttcta tgcgattacg cattcgaaaa tggataatta tttttcggat tttgaaacca
    69661 tcgcagttgc tgtgttggga acagtgattt tagcttttgc attatttggc tggagacgct
    69721 tgttttacct cgcttttgtc gccttagtta tcgtatctgg ctttttcgtc aatccagtcg
    69781 tagaaggtac aggagcgatt tttgaaaaaa cactggcaaa agaaatccaa aaaatcgatc
    69841 aacaagatcc tggtaaagtt tggttgtctg aagatgaatt atataacttc accccaacat
    69901 tgggagtgaa atccttcaat acagtacgct tttatccaga tatagctgca tggaagaaaa
    69961 tcgatccgaa agaagaatac gagaagtatt ataatcggta tgctcataca agagctttta
    70021 tcgctaagga agatacaaag ttcattttag atcgtccaga tatgttttcg gtcacgttga
    70081 attttgaaga cgcagaaaaa ttagggatcg actatgtgat cagtaaacga ccattaactg
    70141 attacaacca attatatgaa gcgcagttca cgcagctata tggaccggat aaagacggtt
    70201 atatgatttt taaagtagaa tatccggatt atccagatgt accacaaaca caaactcagg
    70261 aaaatcagct agtcgtacag taataggcta gctgagaata atcctgaaat attagtagaa
    70321 aggagagtga agataattca agcaatcgta caaaaatttg tcaacaaaat gagtgtcggt
    70381 ttcttgcttt tgttgctgac gctagtcttt tttgtttgga cagcaggggt aggttttcca
    70441 gcaactttgt ttatatataa caatagttca ttacttatcg cttttagtgt ggctattttg
    70501 ctcattttaa atgtttataa agcaaaaggc gtcgattttc tttatctagg tgttgcgctt
    70561 gcccttttca ttttcttttc ttactttcgg gaattaagaa atgagtcttt atttggagac
    70621 ttactgtttc ctttggttat tttattttgt ttagtaatta aatggatcga gtggacaaaa
    70681 tgggatcgcg gtatttatct gattgtcttt acggtcttcc tcggaatcac gttatatcgt
    70741 gtatttacag agatccaagt accagaagga caaagcatct gggctccagg caattcgctt
    70801 tctgatattt ggatcaatac gaatacaatc ggaagttctt tgatgactct tgctttattg
    70861 atcagtggat ttgccagttc ttttgaacga tggtatgttc gaatcatcgg tgggatagca
    70921 gtcgttgcag cctttttagg tatttgggtc tgccaatcta gaggtgcatt agtcgcgttg
    70981 attgtttttg cgatattgga tcttttacct aaatcgttga tgcgtgtcat tcgtgcgcca
    71041 tttattgcct acacggttac gattctttta gctttgccga tttcttatct ggcggctgtc
    71101 tctgaaaaag tcaatttgtt taccgatcga gaagatattt ggcataaatt ctatcagacg
    71161 ctaggagaaa aatctgaaca agttttacta ggaatgaaaa catttatctt ccagcgagga
    71221 aaccaatttt taggaaacca caatagttat aattctattt tgaatatcta tggattgata
    71281 ggatttggga ttgctgcttt gctgcttatc ctttttattg gtcgcttaac gttgaaagca
    71341 gatttatcca acgggcagat gacttttatc tgggcctttt ttgcggtgat gatgcagtca
    71401 tttatggagg atacattgac ttcataccca tgggttccga ttatttatat cctgttggct
    71461 atggccgttc atcgatatga tgaaccagaa gttgcggagg aattctatgt tatggaagag
    71521 gcatatgacg atatgactta tgatgataca tcttatgagg aacaaccaga tagcgctcat
    71581 tcttccagga tccaacgcta tcattgatag gcacaaaat
//
LOCUS       DARBEC010000003        61376 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.003, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000003 DARBEC010000000
VERSION     DARBEC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 61376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 61376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..61376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.003"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..563)
                     /locus_tag="SCL80_000208"
     CDS             complement(<1..563)
                     /locus_tag="SCL80_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF443 family protein"
                     /protein_id="HEG1106107.1"
                     /translation="METVRLWIFMKNFRSALRYVILESKSGYYLIDTDHYTFWGGYLF
                     PIFNWIIPHKVMKINLPAEEINRLILNKDLQRKREKEHSNVAWIGPAFAVVTSSIVGP
                     MLESIYSDFSIGIRYLFVTLSIAVLLCIKGYVSKKNKDILTLIGVNNLSSTKIILFPE
                     SIKEGSRLLIIFIMLILFSVLFGVMFI"
     gene            complement(750..1400)
                     /locus_tag="SCL80_000209"
     CDS             complement(750..1400)
                     /locus_tag="SCL80_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106108.1"
                     /translation="MFIKGGQKMCFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLR
                     AGLITFISIVGFFLFTVIVFIWRTTAPPLPEITYGEFPFSLEYEIDEERILIEDTVIA
                     EFIGSFRGNVTTQPRRSWSIKLLNNSGELKAFHYEFLIIESEDVTITLSSGSAGYFMN
                     DGGSKPQLTVRDRIAGFMHFSHYDLEEARKILAEYGITLINWEYAPPIRNFFNDYY"
     gene            complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="SCL80_000210"
     CDS             complement(1943..2878)
                     /gene="whiA"
                     /locus_tag="SCL80_000210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEG1106109.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(2912..3910)
                     /locus_tag="SCL80_000211"
     CDS             complement(2912..3910)
                     /locus_tag="SCL80_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEG1106110.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAINR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="SCL80_000212"
     CDS             complement(3907..4791)
                     /gene="rapZ"
                     /locus_tag="SCL80_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEG1106111.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(4926..5657)
                     /locus_tag="SCL80_000213"
     CDS             complement(4926..5657)
                     /locus_tag="SCL80_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEG1106112.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLRPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTALQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSEALDTASEK"
     gene            complement(5661..6926)
                     /locus_tag="SCL80_000214"
     CDS             complement(5661..6926)
                     /locus_tag="SCL80_000214"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEG1106113.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="SCL80_000215"
     CDS             complement(7189..10005)
                     /gene="uvrA"
                     /locus_tag="SCL80_000215"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEG1106114.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQEIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="SCL80_000216"
     CDS             complement(10015..12009)
                     /gene="uvrB"
                     /locus_tag="SCL80_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEG1106115.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQIRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKDAAKALDFETAANVRDMILELKASK"
     gene            12268..13770
                     /locus_tag="SCL80_000217"
     CDS             12268..13770
                     /locus_tag="SCL80_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEG1106116.1"
                     /translation="MKRKHLFHSFVFLMTGLVTFAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            13772..14509
                     /locus_tag="SCL80_000218"
     CDS             13772..14509
                     /locus_tag="SCL80_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1106117.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(14554..14727)
                     /locus_tag="SCL80_000219"
     CDS             complement(14554..14727)
                     /locus_tag="SCL80_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106118.1"
                     /translation="MEKKNQAQLSEGGIDYVLDIINEPMVLSYAMEQVERLLVCAGKQ
                     AMRLIRLGEKIRR"
     gene            14703..15368
                     /locus_tag="SCL80_000220"
     CDS             14703..15368
                     /locus_tag="SCL80_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106119.1"
                     /translation="MELDFSSPYYVVVGQLSDNQQLCQERINPLLELLERVFSYYGQA
                     LLTVHRQQIIILVNRISRASLLSLLDKIQTKFNKMYQLQLNFGIGSLCYTEQETPQSF
                     LHAKQVCEWIAFHQSVNEIRFLEDLDLGIVLPAIPSDQRTLYVKRILKSLTEEEVHLF
                     KKTLACFSKNNGSIKNCSEELFIHKNTLQYRLNKFHSLTGYTPRNYDDYHILKLAFLL
                     VQT"
     gene            complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="SCL80_000221"
     CDS             complement(15450..16616)
                     /gene="dnaJ"
                     /locus_tag="SCL80_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEG1106120.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="SCL80_000222"
     CDS             complement(16767..18596)
                     /gene="dnaK"
                     /locus_tag="SCL80_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEG1106121.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="SCL80_000223"
     CDS             complement(18647..19210)
                     /gene="grpE"
                     /locus_tag="SCL80_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEG1106122.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="SCL80_000224"
     CDS             complement(19304..20347)
                     /gene="hrcA"
                     /locus_tag="SCL80_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEG1106123.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(20581..21747)
                     /locus_tag="SCL80_000225"
     CDS             complement(20581..21747)
                     /locus_tag="SCL80_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEG1106124.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNDHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(21763..21990)
                     /locus_tag="SCL80_000226"
     CDS             complement(21763..21990)
                     /locus_tag="SCL80_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEG1106125.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            22474..23064
                     /locus_tag="SCL80_000227"
     CDS             22474..23064
                     /locus_tag="SCL80_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEG1106126.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            23254..23967
                     /locus_tag="SCL80_000228"
     CDS             23254..23967
                     /locus_tag="SCL80_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106127.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(24082..25092)
                     /locus_tag="SCL80_000229"
     CDS             complement(24082..25092)
                     /locus_tag="SCL80_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEG1106128.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(25129..25611)
                     /locus_tag="SCL80_000230"
     CDS             complement(25129..25611)
                     /locus_tag="SCL80_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEG1106129.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            25761..26129
                     /locus_tag="SCL80_000231"
     CDS             25761..26129
                     /locus_tag="SCL80_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEG1106130.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            26594..27046
                     /locus_tag="SCL80_000232"
     CDS             26594..27046
                     /locus_tag="SCL80_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106131.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(27170..28021)
                     /locus_tag="SCL80_000233"
     CDS             complement(27170..28021)
                     /locus_tag="SCL80_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEG1106132.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(28034..28819)
                     /locus_tag="SCL80_000234"
     CDS             complement(28034..28819)
                     /locus_tag="SCL80_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1106133.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="SCL80_000235"
     CDS             complement(29171..30112)
                     /gene="ribF"
                     /locus_tag="SCL80_000235"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEG1106134.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(30116..31039)
                     /gene="truB"
                     /locus_tag="SCL80_000236"
     CDS             complement(30116..31039)
                     /gene="truB"
                     /locus_tag="SCL80_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEG1106135.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(31407..33769)
                     /locus_tag="SCL80_000237"
                     /pseudo
     CDS             complement(31407..33769)
                     /locus_tag="SCL80_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            34217..34390
                     /locus_tag="SCL80_000238"
     CDS             34217..34390
                     /locus_tag="SCL80_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106136.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
     gene            complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="SCL80_000239"
     CDS             complement(34547..34894)
                     /gene="rbfA"
                     /locus_tag="SCL80_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEG1106137.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(34921..37227)
                     /gene="infB"
                     /locus_tag="SCL80_000240"
     CDS             complement(34921..37227)
                     /gene="infB"
                     /locus_tag="SCL80_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEG1106138.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(37240..37551)
                     /locus_tag="SCL80_000241"
     CDS             complement(37240..37551)
                     /locus_tag="SCL80_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEG1106139.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(37548..37841)
                     /locus_tag="SCL80_000242"
     CDS             complement(37548..37841)
                     /locus_tag="SCL80_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEG1106140.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="SCL80_000243"
     CDS             complement(37863..39038)
                     /gene="nusA"
                     /locus_tag="SCL80_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEG1106141.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="SCL80_000244"
     CDS             complement(39061..39534)
                     /gene="rimP"
                     /locus_tag="SCL80_000244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEG1106142.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(39679..44037)
                     /locus_tag="SCL80_000245"
     CDS             complement(39679..44037)
                     /locus_tag="SCL80_000245"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEG1106143.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(44249..45958)
                     /locus_tag="SCL80_000246"
     CDS             complement(44249..45958)
                     /locus_tag="SCL80_000246"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEG1106144.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="SCL80_000247"
     CDS             complement(46026..47294)
                     /gene="rseP"
                     /locus_tag="SCL80_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEG1106145.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(47455..48255)
                     /locus_tag="SCL80_000248"
     CDS             complement(47455..48255)
                     /locus_tag="SCL80_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEG1106146.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(48252..49064)
                     /locus_tag="SCL80_000249"
     CDS             complement(48252..49064)
                     /locus_tag="SCL80_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEG1106147.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(49259..49816)
                     /gene="frr"
                     /locus_tag="SCL80_000250"
     CDS             complement(49259..49816)
                     /gene="frr"
                     /locus_tag="SCL80_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEG1106148.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(49819..50541)
                     /locus_tag="SCL80_000251"
     CDS             complement(49819..50541)
                     /locus_tag="SCL80_000251"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEG1106149.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(50677..51558)
                     /locus_tag="SCL80_000252"
     CDS             complement(50677..51558)
                     /locus_tag="SCL80_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEG1106150.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="SCL80_000253"
     CDS             complement(51657..52439)
                     /gene="rpsB"
                     /locus_tag="SCL80_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEG1106151.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            52798..53277
                     /locus_tag="SCL80_000254"
     CDS             52798..53277
                     /locus_tag="SCL80_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEG1106152.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            53494..54585
                     /gene="phnW"
                     /locus_tag="SCL80_000255"
     CDS             53494..54585
                     /gene="phnW"
                     /locus_tag="SCL80_000255"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEG1106153.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            54578..54706
                     /locus_tag="SCL80_000256"
     CDS             54578..54706
                     /locus_tag="SCL80_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1106154.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            54710..55255
                     /locus_tag="SCL80_000257"
     CDS             54710..55255
                     /locus_tag="SCL80_000257"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEG1106155.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(55709..57400)
                     /locus_tag="SCL80_000258"
     CDS             complement(55709..57400)
                     /locus_tag="SCL80_000258"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEG1106156.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="SCL80_000259"
     CDS             complement(57820..58767)
                     /gene="arcC"
                     /locus_tag="SCL80_000259"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEG1106157.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(58882..59901)
                     /gene="argF"
                     /locus_tag="SCL80_000260"
     CDS             complement(58882..59901)
                     /gene="argF"
                     /locus_tag="SCL80_000260"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEG1106158.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="SCL80_000261"
     CDS             complement(59992..61221)
                     /gene="arcA"
                     /locus_tag="SCL80_000261"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEG1106159.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
BASE COUNT        17061 a        13103 c        10625 g        20587 t
ORIGIN      
        1 tctataaaca tcaccccaaa taaaacagaa aacagtataa gcattataaa aattatcaac
       61 aaccttgatc cttcttttat agattcagga aacagtataa ttttagtact agataaattg
      121 tttacaccga ttagtgttaa aatatcttta ttcttcttgc taacataacc ctttatgcac
      181 aaaagaacag ctatacttaa agtaacaaat aaataacgta ttccaataga aaaatcacta
      241 taaatactct ctagcatagg accgacaatt gaagaagtca ctactgcaaa tgctggacct
      301 atccaagcaa cattagaatg ttctttctct cttttccttt gtaaatcttt gttgagtatt
      361 aatctattta tttcttctgc aggcaagtta attttcataa ccttatgggg gattatccaa
      421 ttaaaaatcg gaaaaaggta gcctccccaa aaagtatagt gatctgtgtc aatcagataa
      481 taacctgatt ttgattccag aataacatat ctcaaagccg aacgaaaatt tttcatgaat
      541 atccataatc tcactgtttc cacacctaat tcctccaatt ccaattaaat ggatttatag
      601 catcaccaat tatgttaact gcctgaccaa cctcattgat aaaattggca ccactatccc
      661 atgcttcaac aatgctatat atctatcaaa caaaaaatgg aaaactaaat actcgcgcac
      721 ataaatttta cattctgttt tttataagac taataataat cattgaaaaa atttcttatc
      781 ggtggcgcat attcccaatt gattaatgtg atcccatatt ctgcaagtat tttacgagct
      841 tcttctaagt catagtgaga aaaatgcata aaacctgcaa tacgatcacg gactgtgagc
      901 tgtggcttgc ttccaccatc attcataaaa tatccagcag agccactact taaagtgatt
      961 gttacatctt cactttctat tattaagaat tcataatgaa atgctttcaa ctctccacta
     1021 ttatttaaaa gttttatact ccaacttcgt ctaggttgag ttgtcacatt ccctctaaaa
     1081 cttcctataa attctgcaat caccgtatct tcaatcaaaa ttctttcctc atctatttca
     1141 tattcaagac taaacggaaa ttctccatat gtaatctctg gcaatggtgg tgctgtagtt
     1201 ctccaaataa atacaataac tgtgaataga aaaaatccaa cgatcgatat aaatgttatg
     1261 agacctgctc gaagtaaaac ccgatataat aaaaatgctg tatttcgctt tgctatatct
     1321 agaaaaataa aaagtactgc aatcataatg atagtaaaag ttgtaaaaaa gcacattttt
     1381 tgccctccct ttataaacat cgattctcaa attctaaacc aaatcttgat agttcaagtt
     1441 caggtataac gtataccaac catacatccc aattactatt tatgaactta tgattatcac
     1501 tgaataaatc actccatccc cttgttaaaa cttctacatt aattacacct ggttgttttc
     1561 gactagctaa tccaatgaac ttatcaacca tccttatcca ccaaccacga gaatcttgac
     1621 cagaagtaag aaaaacagtt ctcaaggttg atcgagcagt tccttccagt ttttaattaa
     1681 tctccagtta tagataaata cttgatccta tctatatcag gacttctata ttaagcaaaa
     1741 gtttatgtat aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat
     1801 taaaaaacaa tagaagcctt tcaacattat gttactccag taaagttgga attatagttt
     1861 gacggaagaa aagcaagtat agcttttaaa aaaaactgat acattcgcat ctcctgcttc
     1921 atgtatcagt tttttctttg gattaaactg cttcttcacg cagtttttcc gcaaattcat
     1981 ttattttgcg aattcggtga ttaattcccg attttgatat tgcacctgag ggaatcattt
     2041 cccctaattc tttcaagctg acctctggat gttgaagccg aagttctgcg atttcctgaa
     2101 gtttttcagg aagggcatgt agaccgacct ttgcttcgat caattcgatg ttttcgattt
     2161 gtttagaagc cgcatcaatc gtcttgttca gatttgctgt ctcacaattg actagacgat
     2221 tgaccgaatt tctcatatcc cgtacgattc gaacatcttc aaatcggagc attgagttgg
     2281 ttgcaccaat caatgttaag aaatcagcaa ttttttctgc tccttttaaa tacgaaatat
     2341 agccattgcg ccgttcaagc gttcgagcat gtagaccgta ataatttaac atatcgcaaa
     2401 tgtcttggtt atgctgttca taaatagaaa atatttccaa atgataacga ctcgtttccg
     2461 gattattgac tgaacctgag gccataaaag caccacgtaa gtaagaacgc attttttgcg
     2521 aatttcccat aatctcgtcc gaaacgtgtc cattgaacat cataccatcc atgatatcaa
     2581 gatccatcaa cacatctttt gtgccttgct tcaaccggac gatatagaca ttgttctttt
     2641 ttaatttcat ttttttacga acgagtagct cacttcgtac ctgataatga tctttcaaaa
     2701 gagaatagat acgtctggca attgcagcat tttctgtttg gacgttcaaa ataaactgct
     2761 ggttcacaat acctaaagag ccgttcattc gcagcaaagc agctaattcc gctttcgcat
     2821 gttctcgatg aacttccaaa ccagttagtt ctttcttcac atctgcagca aatgacatcc
     2881 tattctcctc ctttctccaa caataaattg cttaatattt tgaaccgaaa actaaacgga
     2941 acaattcttc tactactttg tctccatcgt ggaagacacc accatctttc aattctaaaa
     3001 agtcggtgga aatcactcga catccttcat ttctcaatcc gttgaagtcg tgtgcgactt
     3061 gtactagata ctcatcatag atctctgggt ccatataatc ttcaggcact ttttccgtat
     3121 tgaccaatac tgtatcgata aaaggctgat tcaaatgctt gtgtaaaaca cgtatatgat
     3181 cggcatctgt gaaatgttcc gtttcccctt tttgggtcat gatattacag atatagacgg
     3241 tttcagcagt tgtcttgagc attgcttcgc cgatttctgg tatcactaaa tttggaagga
     3301 tactggtgaa taaacttccg ggtcccaaaa cgaccatgtc ggcgtccata attgctgaaa
     3361 cgacttttcg agccgctttg gcttcacttt tatcatttgt gttgcagaca tacacacggt
     3421 caatggtttt acggttgatc gcgattttag attctcctac tgcagtcgtt ccgtctttaa
     3481 aaacagcatg caaggtcaaa ggagtttctg aagaaggata aacatgtcca tctacatgca
     3541 tcatttttgc taataattga atcgcctcat aagtgcttcc ccgcatctcg gaaactgcgg
     3601 caatgatcag attaccgatc gtatgattgg ctaaaaactg atcttccttt ttgaaacggt
     3661 attggaaaat atcttcatag aattgtggca tatccgataa cgccactaag acatttctca
     3721 agtcaccagg aggagccata gcgatcgatt ctctgattgc accgctgctt ccaccatcat
     3781 ctgcaacagt cactactgct gtgatatctg ctccttgatt tcgtaaactt ttcaggataa
     3841 ctggcagacc agtgccaccg ccgaccacta cgatctttgg ttttcttatc cggtaggttt
     3901 tcattttcac gaccggttca ccgtctcttt ccttttatcc ttgtcccgat gtgtcacatt
     3961 gactttatag tttttcccta aagcatttgc caaacgttca gtcaatgcca cagaacggtg
     4021 ttgaccgcct gtacagccga ttgcaactgt caagctgctt tttccttctt taatgtatcc
     4081 aggcaataca gtttccagaa gatccaaaaa tttatggtag aatgattgcg tttcatcaaa
     4141 ggacatcaca taatcataga ctggctgatc ttttccggtc aatggtctta gttccgggat
     4201 ataatgaggg ttgggcaaaa aacgcacatc catcacaata tccgaatcga tcggcaaccc
     4261 gtatttaaaa ccaaatgata caaattcgat ccgaaatcct tggtctccct ttgatttgaa
     4321 tgactcattg attttttcac gtaattgtcg tggtgtcaat gttgtcgtat caatgatcac
     4381 ggaagcttgg gctttcaagt cgtctagtat cgcccgttct ttacgtatcc cttcagtgat
     4441 caaaccgtcc atcgccattg gatgtgctcg tcttgtttct ttgtagcgag ccactagttc
     4501 ttcgtctgaa gcatctaaaa ataagataga agtgtcgatg aagttcgtat tttcgatttc
     4561 gatcagcatg ttttggattt cttcaaagaa ggaacgagaa cgcaaatcca ctcctaaagc
     4621 catttttgtg actttacctg attctttgat caattcccag aattttggaa tcaatcgagg
     4681 cggcatatta tcgatacaaa aatagcccat atcttcaaaa ctttggatag ctactgtttt
     4741 ccctgcacca ctcatacctg ttatgatgac taattgcaaa ttatctgtca tatttacgaa
     4801 cccctctcac attgtgcata ataaaattat atcatcccgg gcgtatcatt accagttttc
     4861 tttttctttt acaaaaagat ttggaccaaa tatcttttag gatatttgcc ccaaatccaa
     4921 tttttttact tttccgatgc tgtatccaat gcctcgcttc tttcagctaa tgcacgttcg
     4981 atcgttcgat ccgctaaaat agattgagca tcgatcactg ggtaatgatt atcttcagca
     5041 aattcatgca tcaaagataa ttctgtgcaa cctaggatga ctttttcaca gtttaggcgc
     5101 tctaccgctt cctctaagat ttcgtaatag agctcttgat tcaaataatc cgattccttg
     5161 atctcatgat agatcaggta attgattttt tcttgaagcg cagtatcagg aatcactgtt
     5221 tcgaaaccaa gatttttgac ttcgcgttca tagattccag ctttcatact tccttctgtt
     5281 cctagaatcg ctactctccc tgtcgtatgc tgacggacaa gttcatttgc tgcttctctt
     5341 ggcatatgca agataggaat atccgttgcc gcttgtaact cttcaaaaaa gtagtgtgcg
     5401 gtgttacaag tcaacacaat gaagtttggt cgcaataaat tttgtttctc aatatcatcc
     5461 agcagaaacg gcattggatt ttcttctgat cgatctaaaa tataagctgt acgatcagga
     5521 actgttgcat gattgaataa gacataattc aaatattctt gatcttttgt agctttcgtt
     5581 cggtggttga tcaagcgaac aaaactttct gtcgccatcg tgcccattcc gcctaaaata
     5641 ctgaaaaaat tctccatgaa ctagccttct ttcacatgaa aatacttttt gaatcttgga
     5701 atatagttat ggaacatata tttcatcaac aaccagcgct tgattgatcg gtctttttca
     5761 taaaatacag tagtaccgta acgattttct ttgatcattt gctcagctag ttttttgtct
     5821 tcattttccc gagcatattc taaaaagatt tttttgggca cacccatcca taatcttgct
     5881 ttatctgatt gattcgttcc ataggtcgtt tcaacaaagg gtttgttgaa cactcgatct
     5941 tctgtgacaa aacgagccag attcaagccg ttcaatgtca caaagaaact gctacgacct
     6001 tgtctcaaat tgatttcaaa taatttatat tctccatctc taggatcata cttcatatcg
     6061 aaattggcaa agcctgtata ttcgattttt tccaaaaatg ccttaatggt ttgatagatt
     6121 ttttcgttat aatctggcat gatcacaacg taattaccaa tcgatgctgg tgtaggatct
     6181 tctaatagtg gatgaccaag gcacatcatc cgaacttgat gatcttcatc tacatacgca
     6241 tttagcacac gcatattact atcatcaccc gggataaaat cttgtacaat catttcactt
     6301 ttataaccgg cttcgtatat ccggcctaaa attaaatcga actcttctct agtctctaaa
     6361 ataaaagctt tttttcttcc ttcaaattgg acagaaagat actccacact gtttgcaggt
     6421 ttcaaagcaa ctgggaattc aaaaggcaat tcctgttcca aatgtccatt tactaacatt
     6481 tcttctctga cgatcaatgt tttaggatac ggaagatcat attcttcaca aacttcataa
     6541 aagcttactt tattgatcag cctttcaaat aaagaataat caatatatgg acagataaaa
     6601 tattctgaca gctcttgttt atgttgggag atcaattctg catatccatc accacaagca
     6661 atcaaaagat attttttact cttatcagaa tatctttctt ttgccaaacg aagcatcgtc
     6721 tcgataaata cagggtcttt atcaaatcct ggaataactt ctacattgac aattttactg
     6781 tagcgggtag gggctaactg atccgaagca tatgcttggc agattttgcc gtatgcttca
     6841 ttaaaagaac gtgccatacc ataaacgttc atgtcgcttc ctaataatat agggataaat
     6901 tcttcatttt caatactgtt catcattatc tctcacttac tcataatctc ttgtttttct
     6961 tttccaacta cacaaggaat atcataccat aaagaaaaaa gcaatcaaag gaaagtcctc
     7021 cgattgctca attctttttt gaacggattg aacttatagg aagtttttat ctataccaat
     7081 cagtgtagaa agaactacca tgtaatgatt ctagaacact gatattaata aaacaagcaa
     7141 aaattcattt ttttatgttt ttttaactat ctcagctatc tatcctcttt ataccattat
     7201 ttttttcaaa tagtgacctg tataactttc ttttacgtta atgatttctt ctggtgttcc
     7261 agtcgcaagg atcgttcctc caccttctcc accttctggt cctaaatcaa tgatataatc
     7321 tgctgttttg attacatcta gattgtgttc aatcactaaa actgtgttac cagcatctac
     7381 taatctttgt aatacatgca acaagcgggc gatgtcatcg ctatgaagtc ctgtcgttgg
     7441 ttcatctagt atatagaaat tctttccatt agagattttg tgcaattcac tggcaagttt
     7501 catccgttgt gcctcaccac cggacaatgt cgttgctggt tgccccatcg tcacataacc
     7561 taagccaaca tcaacaatcg tttgcagttt gcgatgaatc tttggaatgt gcttgaagaa
     7621 ttctacagca tcctctactg tcatttccaa aatatcagca atgctttttc ctttgtaatg
     7681 cacttctaat gtttcagaat tatatcgttt accatgacat acttcacaag gaacatagac
     7741 atcaggcaaa aagtgcattt cgatcttaat aattccatcc ccgcgacaag cttcacaacg
     7801 accgcctttt acgttgaaac taaaacgccc tttcttataa ccccgcattt ttgcctcgtt
     7861 cgtttgagca aataacccac ggatatcatc aaatacactt gtataagtcg ctggattact
     7921 tctcggcgtc cggccgattg ggctttgatc gatatcgatg atcttttcga tactttcgta
     7981 gccggaaatt gtcttgaatt tacctggctt agcagaattc ttatttaatt tttgcgctaa
     8041 cgatttcttt aagatactat tgaccaatgt actctttcca gaaccagaca cacctgtgac
     8101 tgctacaaat tctcctaatg gaaattcaac actaatattt ttcagattgt tttctgaagc
     8161 accagtgatc ttgattgctt tcccattacc tttacggcgt tcttttggta cagggattga
     8221 ttttttccct gaaagatatt tccctgtcaa agaattttca tcattagcta cttcttcagg
     8281 agtgcctgca gccacgattt gtccgccttg ttctccggcc ccaggtccga cgtcaatcaa
     8341 ataatctgct gcccacatcg tatcttcatc atgttcgact acaacgaggg tgttgccaag
     8401 atcacgcatt ttctttaatg agccaatcag tcgatcgttg tcgcgttgat gcaatccgat
     8461 cgatggttca tctaaaatat acaaaactcc agaaagattc gatccaattt gtgtagccaa
     8521 tctgattcgc tgcgcttctc ccccagataa tgttccggaa gcgcgactca aagttagata
     8581 gtctagtccc acattttcca agaatgaaag tctgtcattt acttctttta aaatcggttt
     8641 ggcaattact ttctcttgtt cagataacgt taagtttgag aagaaatcta ctgctttttg
     8701 aatagacaac tcatttgttt gcccgatatt cgttccattg atttgaacag atagagcttg
     8761 gggattcaat cgataacctt tacatgtctg acaagttaat tctgtcatat aaagacgcat
     8821 ttgctcacgc gtgaaatcac tgttcgtatc atgataacga cgctcgatat tcttcaatac
     8881 cccttcgaaa gtcgtttcta catcgcgaac gccaccaaaa tcattttcat aatggaaatg
     8941 gaaaagatca tctgtcccat tcaagatgat ctcctgatgc tcttccggta attcttcgaa
     9001 tggtgtatct aaatcgatac cgaaactttc acaagcctgc gctaacattt gaggataata
     9061 ctgagagctg attggattcc atggaacgat agcaccttga ctaagtgtct tcgttctatc
     9121 tggaatcact aaatcttgat cgacttctaa tttgacacct aaaccatcac agtctggaca
     9181 agctccaaaa ggcgcattga atgagaacag tcttggttct agttcgccaa cagtaaatcc
     9241 gcaataagga caggcatagt gttcgctaaa caacatttct tcttcaccga tcacgtcaac
     9301 cagagcatag ccgtctgcta atcgcagggc cgcctctaaa gaatcaaata ggcgagaacg
     9361 aatcccttct ttcacaatga tccggtcgat gacgatcgcg atatcatgtt ttttgttttt
     9421 ctccagctca ggcacttcac ttaaatcata gatctcacca tcgactcgaa cgcggacgta
     9481 tccctctttt tggattcgtt cgaatatttt tttatgttgt ccttttttct ttaccacgat
     9541 cggtgccaac acttggatct ttgtgcgctc aggcaaagcc attacttgat ctgccatttg
     9601 atctactgat tgactggtaa tctcgatatg atcgttggga caaatcggat gtcctacacg
     9661 tgcaaacaac aagcgtaaat agtcattgat ttccgtcaca gtacctactg tcgaccgagg
     9721 atttttactt gttgttttct gatcaataga aatcgccgga ctcaatccgt cgatgctatc
     9781 tacgtccggc ttatccattt gtcctaaaaa ctgacgagca taagcagaaa ggctctctac
     9841 gtatctccgc tgcccttcag cgtacagcgt atcaaatgct agggaacttt ttccagaacc
     9901 ggacaaacct gtaacgacca cgaatttatc tcgtggaatc gttacatcta tatttttcaa
     9961 gttatgggca cgtgcaccat gaatcgtaat tttatcgtta gccatgttgt cctcctactt
    10021 tgaagctttt aattctaaaa tcatatctcg cacattcgcg gctgtttcaa aatccaaagc
    10081 ttttgccgca tctttcattt ctttttctaa tttcatcaat aacgtgtctt tttcttcctt
    10141 cgtcatttct tcgtacgaga cttgtaccgc ttcttttgta tcatcttccg attctttcgt
    10201 aatagaaatc aaatctcgga tttctttaat gatcgttttt ggaacgatcc catgttcttc
    10261 attgtacttc tgctgaatcg tacgacgtct tgacgtttca tccatcgcta gacgcataga
    10321 atctgttact ttgtctgcgt acatgattac tttaccttct tcattccgag cagctcgtcc
    10381 aatcgtttgg actaaggaac gttcactacg taaaaagcct tctttatccg catccaagat
    10441 agcaacaagg gaaacttcag gtacgtctaa tccttcacga agcaaattga ttccaactaa
    10501 cacatcaaat tcacctaatc gaagatctcg aatgatttct gttcgttcca gcgttttgat
    10561 atcactatgc aaatatttga ctttgatccc taattctttg aaataatctg tgaggtcttc
    10621 tgccattttt ttcgtcaatg tcgttacaaa cacccgctgg tctttttcga ctcgttcatt
    10681 tatttcgcct actagatcat cgatttgtcc catgattgga cgaacttcga tcactgggtc
    10741 aagcagccct gtcggacgga tgatttgttg gataacggta tctgtttgtt catgttcata
    10801 tggtcctggc gtcgcagaga cataaatgat ctgattgaca tgtttttcaa actcttctaa
    10861 ccgaagtggc cgattgtcta aagcagatgg caaacggaaa ccgtaatcta ctagcatctg
    10921 ttttctcgct cggtcaccat tatacattcc tctgatttga ggcatggtta cgtgcgattc
    10981 gtccgccacg atcaaaaaat cttcaggaaa gaaatccaat agcgtataag gcggttcacc
    11041 ttctttacgt ccgtccatat gccgagaata attttcaatt cccgacgtgt agcccatttc
    11101 taacatcatt tcaatatcgt aattggttcg ctgttctaaa cgttgagctt ccagaagttt
    11161 gttttcattt cgcaagacag ttaaacgctg ttctaattcc gctttgatat tcgctacggc
    11221 atgctccata tgttcatcat tcgtcacaaa gtgagtcgcc gggaagattg ctacgtgttc
    11281 cgtttctcct aaaacttcac ctgtcagtgc attgacttct cggatacgtt cgacctcatc
    11341 tccaaaaaac tccacacgta aggcacgttc atctcttgaa gccggaaaaa tctccacgac
    11401 atcaccgcgc actcgaaaac gaccgcgctg aaaatctata tcatttcttt cgaattggat
    11461 actgactaag tcacgaatca actgattgcg atctagttct gctccctgtc gaatcgaaac
    11521 tacttgtttt tggtactcaa aaggcgagcc taatccaaaa atacatgaaa cggatgcaat
    11581 gacgatcaca tcatttcttt ctaataatga actagtagcc gaatgtcgta atttgtcgat
    11641 ctcatcattt acacttgaat ctttttcgat atacgtatcg cttgaaggca cataagcttc
    11701 aggctgataa taatcataat aagaaacaaa atattcaaca gcattgttag ggaaaaattc
    11761 cttgaattcc ccgtacagct gaccagccaa tgttttgtta tgggcaataa tcagtgttgg
    11821 tttattcact ttctcaataa gattggaaat cgtatatgtt ttccctgttc cagtcgcacc
    11881 taataagatt tgtgcttttt tcccgccgac tacgccatcc actaattggt taatcgcttc
    11941 aggctgatca ccagccggtt ggtatttcga gacaagctca aagtgtcggg aggtatctct
    12001 ctcaatcatt cttcgtcctc cttttcattc attccttttc attttacgac caataaaaag
    12061 aaaaggaaag caaaggcatt ccttactaaa aaattatacc acaccgaaca tactttcgcc
    12121 acttttttat cttattatac atgttatgtt ttataacata aaatgttaga aactcgcatt
    12181 tttttcattt tttcgagctt acagtcttgt ctgattcagc acttttcgcc tataataata
    12241 aaatattgga aatttaggag gttgattatg aaaagaaagc atttatttca ctcatttgtt
    12301 ttcttaatga caggacttgt gacatttgcg tttggtcatg ttgcacaagc tgacaacact
    12361 acaacggcag agtcaagcac aagcgagaca atgcaaaaaa tagagcccaa aaaagacgta
    12421 tacgtaattg ccagtgactc tgcttttgct ccatttgaat ttcaaaacgc aaagggcgag
    12481 tacgaaggca tcgatgtaaa attgatggaa aacatcgcca aactgcaagg atttaacatc
    12541 cgaatggact accgtggttt ctctgctgct ttgcaagctt tggaatcagg acaagcagac
    12601 ggaatgattg ccggaatgac tgtaacagat gaacgaaaga aaagctatga tttttctgat
    12661 tcttactttg atagcgggat ccaattagct gtcaaaaaag gagatacttc gatcaagtct
    12721 tatgaagatt tgaaaggaaa aacagtcgga gcaaaagttg gtaccgaaag tgctgaattt
    12781 ttaagcgacc atgaatctga atatggctat tctatcaaat attttgatgc tgctgatcaa
    12841 ttgtacgacg ggttaaaagt tggcgcaatc gatgcaatga tggatgatta tccggtaatc
    12901 ggttatgcga tcaaacaagg acaagaattg gaaacaccga tcaaaaaaga atctggcgga
    12961 aaatatgcat ttgccgttaa aaaaggacaa aatcctgagc ttcttaatat gttcaatgaa
    13021 ggattgaaag aattaaaaag aacaggcgaa tacgataaga ttattaaaga atatgtagcg
    13081 gatggttcag aaacttccag tgagtcttcc actgctgatg aatcaacatt tcttggactt
    13141 atccaaaaca actatcaagc attgttgaaa ggactttgga gaacaattct cctaactttg
    13201 atctcctttg ctttggctat agtaatcggt gtgatttttg gattattcag tgtagcacca
    13261 atacgcgctc ttcgcacaat tgctagtatt tatgtcgata tcatccgagg aattccaatg
    13321 atggttcttg cgttctttat tttctttggg ttgcctggta tcgttggatt tacgattcct
    13381 gacttccttg ctggtgttat caccttgact cttaacgcaa gtgcttatat cgcagagatt
    13441 gttcgaggcg ggatcaacgc cgttcctgtc ggacaaatgg aagcttccag aagtttaggt
    13501 ctttcttata atcggacaat gcaaaaaatc attttgccgc aagcgatacg catcatgatc
    13561 ccatcattcg tcaatcagtt cgttatctca ttaaaagaca caacgatcat ttccgcaatc
    13621 ggtgtagtcg aattgctgca aactggtaag atcattgttg cacgtacaac acaaagtacg
    13681 tatgtttact tgatcattgc gatcatgtac ttgattttga tcacagcatt gacaaaatta
    13741 gcaaaagttt tagaaaagaa ggtgaagtaa gatggcagaa aaaatcttag ttgagcattt
    13801 agtcaaaaaa tacggcgata acacagtatt aaacgacatc aatgtatcca tcaacgaagg
    13861 agatgtcgtc tgtatcattg gcccttcagg ttcaggaaaa agtacattct tgcgttgctt
    13921 gaatcgttta gaagaacctt ctgacggtga aattattatt gacggcgccc acttgatgga
    13981 taaaagcaca gatatcaact tggtgagaca acatatcgga atggtattcc aacactttaa
    14041 cctattccct catctttcta ttttggaaaa catcatttta gcaccgatgg acctgaagaa
    14101 agaatctaga gaagaagcag agaaaaaagc aatcaaatta ctgcagacag ttggtctaga
    14161 agataaaaaa gatatgtatc ctgaaatgct ttctggtgga caaaaacagc gtgtggctat
    14221 tgctcgtgct ctagcgatga accctgacat catgcttttt gatgagccta cttctgcact
    14281 cgatcctgaa atggtcggtg atgtcttgaa cgtcatgaaa aagttagcag aacaaggtat
    14341 gacgatggtt atcgttacgc acgaaatggg gttcgctaaa gaagtggcca atcgcgtcat
    14401 gttcattgac gacggaaact ttttagaaga tggaacgcct caacaaatct ttgaaaatcc
    14461 gcaaaatgaa cggacaaaag atttcttgaa taaagtatta aatatttaat aaaaaccaaa
    14521 aatgcacggt cgccatgaag ggaccgtgta tttttatctt cttattttct cacctaatcg
    14581 tatcagccgc atcgcttgtt ttcctgcaca aacaagcaat cgctctacct gttccatcgc
    14641 ataagaaagt accatcggtt cattgatgat atcaagtaca tagtcgatac caccctcact
    14701 taattgagct tgatttttct tctccatatt atgtagttgt gggacaactt tctgacaatc
    14761 agcaattgtg tcaagaacgg atcaatcctt tattggagtt gctcgaaagg gtcttctctt
    14821 attacggaca ggctttgctc actgtacata gacaacaaat cattatccta gtcaatcgga
    14881 tttccagagc ctcattgcta tcattgcttg ataaaatcca gacaaagttt aataaaatgt
    14941 atcagttaca gctaaacttt ggaattgggt cgctttgtta tacagagcaa gagacaccgc
    15001 aatctttcct tcatgccaaa caagtttgcg aatggatagc tttccatcaa tctgtgaacg
    15061 agatccgctt tttggaagat ttagacctcg ggatcgtttt accagcaatc ccgtcagatc
    15121 aacgaacatt atatgtaaaa cgtattttga aaagcctgac agaagaagaa gtacatttgt
    15181 tcaagaagac tttggcatgc ttcagtaaaa ataacggaag catcaaaaat tgctcagaag
    15241 aattatttat tcataaaaac accttgcaat atcgattgaa caagttccat tctctcactg
    15301 gttatacccc tagaaactat gacgattatc atattctaaa gctggctttt ttgttggtac
    15361 aaacatgaca actacaaaaa aaccaggcga acatatgatt gttcgtctgg tttttaatta
    15421 ttaatgactt agcgttttta ctagaaaaac tacttccgct ttttcccgcc aaaagcatct
    15481 ttaaacttat cgaagaaacc gtcttcttgc tgttcttcaa ctttgatgcc gccagcttca
    15541 gcgaatgcac gtaatgcttc tctttgttgt tcatttagat ttttcggtgt gatcagtttt
    15601 actttgacat gttggtcgcc tgttgcacca ccacgtaact tcggagcgcc ttttccacgt
    15661 aaacggaagt tcgtacctgt ctgtgttcca gcagggatct tcaatttcac atctccatgg
    15721 acagttggta cttggacttc atcccccaaa gctgcttgta caaaacttaa aggcaattca
    15781 tagtaaattt cagaaccttc acgatcaaag atatcacttt cttctacacg gaagaccaca
    15841 tacaaatcac caaatggtcc gccgttttct ccagcttctc cttgaccagc taaacgcatt
    15901 tgttgtcctt cttctacacc agctggtaca ttcactttaa ctgaatgtgc tttcttctca
    15961 tgtcctgagc catgacaggt tggacaaggt tctttgattt ctttccctgt cccatgacaa
    16021 acatcacaag tttgacggct catcactcgt ccaagtggtg tttggcgttc tacattaatc
    16081 gtacctgcac catgacattt atgacaagta gttggttgtg tgcctggttt tgcaccgttc
    16141 ccaccacaag tatgacagat ttcttcccga ttatacttga tttctttttc tacaccaaaa
    16201 atcgcttctt caaaagacaa gtcgaccgta tattgcaaat cagcaccttg acgaggcgca
    16261 tttggatcga ctgaccgacc gcctcctcca aagaaagagt cgaagatatc ttcaaaacca
    16321 ccgaagccgc caccagagaa gccgccgaat ccaccaaatc cgccaccagc tccaccagca
    16381 ccgtaatttg gatctgtgcc tgcatgcccg tattggtcat atgcagcgcg tttttgcggg
    16441 tcacttaaaa tctcatatgc ttctgatact tctttgaatt tttcttccgc atcaggttct
    16501 ttattgatgt caggatggta ttttttcgaa agcttacgat aagctttctt tatttcatct
    16561 tctgatgctc cttttgagag ccctaagact tcataataat cacgttttgt tgccataggc
    16621 tttcctccta ttggttctca tcattttcac gtcgcttgtt atcatatcat attttgtttg
    16681 aaacaaaaac cgatacttca aactattttt caaaagaaaa agccaaagta gcaactttgg
    16741 ccttctctcg aaattcagtg aactgattat ttatcgtcgc catcgacttc ttcaaaatca
    16801 gcatcaacga catcatctgc tccgccttga gctgcttctg ggttttcttg agcttgttgt
    16861 tgtgccgctt gttcgtatag tttaacagtc aagttttgaa cgatttcgtt caatgcatcg
    16921 cgtttagttt tcatttcttc gatgttgttt gcttcaacag cggctttcaa ttcatcgcga
    16981 gctgtttctg cttttttcac ttcttcttcg tccactttgc cttctaattc tttcaatgtt
    17041 ttgtccacag agaatagcaa tgcatctaca tcattacgta ggtcaacttc ctctttacga
    17101 gctttatctg cttctgcatt tgcttctgca tcttttacca tacgttcgat ttcttcgtct
    17161 gttagacctg aagaagattt gatcgtgatt ttttgttctt tttgtgtacc tagatctttc
    17221 gcagatacat ttacaatccc gtttttatca atatcgaatg ttacttcgat ttgtggaata
    17281 ccacgaggag ctgctggaat atctgtcaat tggaatcttc ctagagtttt gttgtcagca
    17341 gccattgggc gttcaccttg taatacatgg atgtctactg ctggttgatt atctgccgca
    17401 gttgagaata cttgtgattt acttgttgga atcgttgtat tgcggtcaat caatttagtg
    17461 aacactccac ccattgtttc gatacctaat gacaatggtg taacgtcaag taaaacaaca
    17521 tctttcacat caccagtgat cacgccacct tggatagcag cacccatagc aactacttca
    17581 tcagggttaa ctgatttgtt tggttctttt cctgtttctt tacgtactgc ttctacaact
    17641 gcagggatac gagtagatcc accgactaaa ataacttcgt cgatttctga ttgtgataaa
    17701 cctgcatctt ttagcgcttg acgtactggt acttttgtac gttctactaa gtcagctgtt
    17761 aattcatcaa attttgcacg tgtcaatgtc atttccaaat gcaatggtcc agattctcct
    17821 gctgtgataa atggcaagct gatttgtgtg cttgatacgc cagataaatc ttttttcgct
    17881 ttttcagcag cgtctttcaa gcgttgtaaa gccattttgt cttgagacaa gtcaatgctg
    17941 ttttcttttt tgaattctgc taccatgtag tcgatgattt tgttatcaaa gtcgtcccca
    18001 cctagatggt tgtcgccagc tgttgacaat acatcaaaga caccgtctcc tagttcaagg
    18061 atagatacgt caaaagtacc gccaccaagg tcgaatacta agattttttc gtcgcgatct
    18121 gttttatcta aaccataggc aagagcagct gcagttggtt cattgacgat acgttctact
    18181 tctagaccag cgatcttacc agcatctttt gttgcttgac gttgtgcatc attaaagtaa
    18241 gctggcacag tgataactgc tttttcgact ttttcaccta aatagtcttc tgcgaatcct
    18301 ttgatatatt gaaggatcat tgctgagatt tcttgtggtg tatatgattt accttctaca
    18361 tctactttgt aacctgcttc accgatatgg cgtttgattg atgaaatcgt atttgggttt
    18421 gtcacagctt gacgttttgc aacttcccct acttggattt ctccattttt gaatgataca
    18481 acagaaggtg ttgtacggtt tccttctggg ttagcgatga tttttgcttc gtttccttcc
    18541 aatactgcta ctgcagagtt agtcgtacct aagtcaatac cgataatttt actcataatt
    18601 aacgtctcct tctttaattg aaagtgtaag ttttatatcc gcacaattat tgtgcaacaa
    18661 tgaccatggt tggtcttaac acgcgatcat gtaatttgta gccttcttgt agtacttcta
    18721 caatcgtatc ggctggtgta tcttcagtcg ctggaacagt ttgtactgct tgatgaagat
    18781 taggatcaaa ggcttctcct ttagcaggga ttttttcaat tccttcttct tccagtgcgt
    18841 tacgcaaact ttccaagacc atttccacgc cttttttcaa acctgcccct tgatcatctg
    18901 acacttctgt ggctaatgca cgttccaggt tgtcaattga tggaagtaat tttttggcta
    18961 aatcttgtga acggtatttt tgtaattgct cacgctcatt ttttcctcga tttgccatat
    19021 ttgcgatttc agcacgagcc cgcaagtatt tatcttccat ttcttctaac tcagctttta
    19081 atttcgctgt ttcgaattct tccgcttcag cttctgaaat acccgaatct tctgcagctt
    19141 cgacatcgat ttccggttct ggctgagcat ctgtcatttc tttatctagt tcttcttgat
    19201 tttctttcac gatacagtct tcctttcatt attcagtcag ctgtttgatt gctcttacta
    19261 agaaagagag aacgatcagc tgcaaatagc ttcgttattc acattacgag tttgacaagt
    19321 caagcgaacg gtagtaatca gcaagtttag ctgctagttc tcttcgataa acatcgacta
    19381 gaccgaacat ccgagaataa ggcatactag taggccctaa caaggctatt gtacctcttc
    19441 catgaccatc tatctcgtaa cttgcttgaa tcatactcat attctgcaat aaatcattgc
    19501 ctaattcagc accaatcttg atatgaatat tgctatccat cggagtgatc atttgtgtaa
    19561 gtccgtctga gtctttcata aaagaaaaca tcgatttgaa ctgatcaaga tcttgacggg
    19621 gctcataatc caagagattc atttgtccac tgacataaac cttatcttca aaaacatgac
    19681 caagaacggt gtcaaataac tgagacatcc cttctgtcgt ttggaaatat ttatgaagaa
    19741 tcatcggaat ctccgtacga agtttatggt acaccgtcat cagaggatct ccgactagac
    19801 ggtcgtttac aatctgtacc attttttcta gttcctgact tcccatatat tcgggaagag
    19861 tgaacacttg gctttctaca ttccccatat ctgttacgac gatagcaata atctgtcgat
    19921 tattcaaggg tacgatacga aagccagtca gtcggcggtc ttttatttca ggtcccaatg
    19981 aaaaagtcgt atagcttgtc agctcagata atatctcagc agattgctta ataatctcat
    20041 tgatttcatg aaattctctg cctagagact gacgaatcag cagcaaatca tcttctgcta
    20101 cgcgagcagg ttttaacaaa tgatccacgt aataacgata gcctaaagca gatggaatcc
    20161 gcccagaaga agagtgagtc ttcaacagca atccttcatc ttctaatgct ttcatatcat
    20221 tgcgaatcgt tgctgagctg gccttgactc cctcagacat caatgttttg gaaccaacgg
    20281 gcacgccact actcgtatag tgttggatga tcagacgtaa aatgtcgttt tgtcgctttg
    20341 ttaacatctt cctcacctct cattagcact catatattct aagtgctaat ccacttataa
    20401 atatagcaag aagcccttct cctgtcaaga gaaagactcc attttttagc actctaaaca
    20461 caagagtgct aattctaaat aattgatgta aatccagtta tatcaacgtt tcaatagtcc
    20521 tttattgtat ttttgaactt tttttattta aattcagata aaaaacaaat attctttaaa
    20581 ctatttttca aacaaaaagc gctcaaacac attgttcccc aaaaaggtcc ctcttggtgt
    20641 caaataaaca cggtcttctt cattaacaag taacccttct tgctctaatt cccttagcac
    20701 atcaccgtac accgattcaa ttgttttttg atagcgatca tagaaatgtt ttttagtaat
    20761 ccctgttttt tttcttagtc ctaagaacat ttcttcttct atttgatttt ttcgtgtcaa
    20821 tttttcttct tccaataccg ggcgttcatt tgctcgtaat gggcgtaaat agtgttggat
    20881 tggtcctttg ttgcggtaac gaatatctcc aagataaccg ctagcaccag ctccaaaccc
    20941 aaaatagtgg tcattgttcc agtacatgag gttatgttga gattcatgtc cggagagccc
    21001 aaagttactt atttcatatt ggtgacgtcc atgcttttcc attgcttcaa tcgtctcttc
    21061 aaacattctg gtttccgttt cctgatcagg cagttcgagt cttccttgtc gtacccagtt
    21121 catgaacatt gttttgtttt cgaggatcaa tgagtacaaa gaatagtgag gcagatccaa
    21181 agccaaagct ttttccaaag tatccagata tccttctagt gtttgtcccg gtaatgcata
    21241 aatcaaatcg atgcttacat tggtaaaatt ttctgcttct aaaaattgca tagtttgata
    21301 cacatcttct gcggtgtgtt tacgtccaat ttttttcaat aagcgattat caaatgtctg
    21361 cactcccatg gataaacgat tgacgccata gtttttcatt acttgaagtt tttcacgggt
    21421 caaatcacct ggattcgctt ctacagtaaa ctcattacca ttttgaaaag gcaggatctc
    21481 tctagcacct gataacagtc gatcaagttg tttagcagat aaagaagttg gtgtcccgcc
    21541 ccctacgtaa agtgtttcta aattcgctgt cggatgactt tctagcatta ttttcatttc
    21601 attcagcagc atttccacat actcatctac tggctgccct tctaaaaaaa ctttattaaa
    21661 atcacagtaa tagcagatat gctcgcaaaa tgggatatga atgtacgcag aaaccttgtt
    21721 ttctgcggga tttattgttt ctatcattga aattctttct ccttacgtcg aaaagtcgtg
    21781 cctgttttcg accttcttaa aatcgcgttc aagcaccaac tcattgaaat aagccgcata
    21841 tttagaatca aggaaaccta gctctaaaaa tgcttgctta ttgtcatcgg agctacacgg
    21901 tgcttttaca gcctctcgat aaacggtaat cgcaaagctg accttcggca ttaagtcaaa
    21961 atctgcaaaa atatgaaaag ctattttcac agattcctcc ttaagtgctc agaccacaac
    22021 gctttgctta caacctctca tatacgcata ctaaacgtat ttggcgaaca aatccactaa
    22081 aaaatccaag aatcgacttc ttggatgatt ttttctttca ttaaactggt tacctagtta
    22141 tatatctcta gggttgtttt catgtctgag tgcccgatat gatttatttt tcgtttctat
    22201 aattactttt tgccttccag tttcttttaa tattgtactc aagcaagtgg gaattggttc
    22261 ttttgtcatc catgtatgca aaatgttata atgctggtaa acaaaaatga ttacctgtca
    22321 tttttattta gttcgtattt aattaagttg actatctgta tactctgtct aaaacttaag
    22381 aattgttgtt ttcgctgtcg ctacccaatt ttaatgaatg tctgtaggtt aaaactgttg
    22441 tccttgttat agatagtaaa ggagagcttg aaaatgaaaa ctgcgattac gtttctatta
    22501 gaggaatatg ccgattggga aggtgcttat attactagca cactaaacca aaatgagaaa
    22561 tggcaaaaca aaactgcttc tatccaatca gaggtcactt ccattggagg attaaaaaca
    22621 aaaatcgatt acttaatcga acaaatacct gaaaaagttg acctttttat tatgatcggt
    22681 ggcaattcat gggacataaa aaatcatcat ttatatcaaa taatcgataa attgttaaaa
    22741 acagacgtac cagtagctgc tatttgtggg gcagttgatt atttagctca gagcggatta
    22801 ttgcaaggtt acaaacacac cggaaatgcg caatttcctt ggttagaatc ggaaaattat
    22861 cagaataaag aagattttat agaacaacaa gctgtgaaag atcgtaattt gattactgcg
    22921 aatggaacag ctcctataga attcacagac ttgattcttc aagcagtcca ttttgaaaca
    22981 gaagaaataa gagaaaaaac gattcgtcta tacaaacttg gattctatga ttactataaa
    23041 aagtacggag atcctttctc ctaatcattc tcttcaatta aatactcctt atcaatagtg
    23101 tgacaagtaa ttttactgat gattatttgt cacacttttt ctgacattta tcaaagaaga
    23161 tacactctat ttatattcat agacaaggtt ttacggattc aaaaattcag gattatgcta
    23221 tactggtatt aaaaaaataa tgaggtattt tatatgaaaa agttaggttt tattttattg
    23281 ttggtctctt tattccttgt aggatgtacg tctcctagta aagaaaatag tgatccatct
    23341 gcgaccacag acaaaagttc aatcagacaa accagttcag ctgaaaaatc aagtactcag
    23401 aaatcagctt ctactgaaac gaatacatct tcaacaagca gtaaagtctc agagcccaat
    23461 acttcttcta catctagtag tacagttgag acaaatattt cttcttcaac aatagacaca
    23521 acctcctcac agtcttctca gtcaatagat ccgttagcag gatattctga tctacaaatt
    23581 gagtacgcac gtgtttggct agctattaga ggtaatactt ttagagatac atcgggtgat
    23641 ttcgaattac atgttattca ttatcctgca ggaacaccta tcgcccctta tgatgcaggg
    23701 agtgcagtct atccgaaaga tgtggttatg ctcactggga aatacagtta ccagggagtt
    23761 ttagtgtatt caagtaacca tgacgggaca atcactagtt atccagttcc ttcacattgg
    23821 caaatgcctg cagatcaaac atcttatcca gcgttcatta gagaaacaac tcaagaaatc
    23881 attgataatg catctgtagt agctattcct acaggaaatc ccaatgacgt gaaacagtta
    23941 atcgatgtga cagttataga taattagact ttctaatatg ttatacaaaa agaagctgac
    24001 agcattttgt cagcttcttt cactttcttt tctatttaga tttattggtc tttgatagac
    24061 tccaaggcag gaaaaaattt tttaaataaa cgtttgcaaa acagtcgctt ttctatcttc
    24121 taccgctact tctgcatagg caccattaat caatgtcagt tgtggcggta catgaccaca
    24181 atcaatatca tacaagatcg gtagttttaa ttcctttgac agatcttcgt atacatcctt
    24241 cattttataa tctaacaaat cctctgccgc acttctaccg aacatgatcc ccgagctgcc
    24301 atcaaaccag cctgccagtt tcatctgaac aagtgatctt cttaaatcaa ctgacgatag
    24361 ctcacagttt tctagaaacc acaagatcgg ctttccatca ttatactttc ttttaaaaga
    24421 ttggacatct ccataaggag taccgattaa atgcctgatc gtatcaatac aaccacctaa
    24481 taatcgtccc ttcatcctta ctgcatctcc gctgactgat tgccaatttg ttggttcagt
    24541 gagatggaaa acagaagatg atgaatgctc atggggccac actttttgat acttttcaga
    24601 agattgctgt agaatcgatt ctccttctct tgttttcagt acactttccc acattgctgt
    24661 cgtttcatcc aagtactctc ctcttaaatc aataaggtta gttccatgtg cagtggctat
    24721 ccctgttttt aatgtgacag caagtagcaa cgtactaata tctgaataac ctactatcca
    24781 ttttggttgt agagcatcaa aatcgacaaa atcgagaatc tcaatcaata attctcctcc
    24841 ccaagtcgga aagatcattt ggatattttc atcttgcatc atttgattga attcatctgc
    24901 tcgttttctt gctggacctg atttttcctt atcttgcgtc caaagcgtct ttccagtaac
    24961 tacttggaaa gatttttctt ccattctctt taaagctgat tcgagtaacg aatggtgttc
    25021 ctctgatacg cctgaggatg gtgcagtaac cccaatcgtt gcatttcttt ctaataatgg
    25081 ataacttatc atgttgcttc acctccaagt atgcactatt ttacttggtt aattgttttt
    25141 ttcgtttgcc ggcatcaaaa atatcggcat ctcaagtagc tggatgattt tgtttaagtc
    25201 aaccgtttgg atgaccatcg tttgatctgg tctacctggt gcaaaaacaa gtttttcatt
    25261 tgataagagc tcttcatcaa taataatcgg aatctgtttt tcataaccaa acggagaaac
    25321 agcgccctgc ttttgtcccg tttgttcttc catgcgttct gatgaaacaa tagaaagcct
    25381 tttaccgacg atcttcccta atgctttgaa atccgtttgt ttcgtagtaa aagtcagata
    25441 tacgtagtta ttatcttttt tatcttttaa gaataagctt ttggtctctg tacctgtaaa
    25501 tccttgttct ttctttatta ccaaagcatc ttcatttgta taaatggctc gatgatgata
    25561 caactcaaaa tatatatttt cgtgtaagaa tttctttgcc aagtcaatca tttttatgtc
    25621 tcttttctat tttattattt gagacatctt atcatataaa tatagcggtt acaacattac
    25681 ttgtgttttg ttcgtttttt atacataact attgtttttc tggtaaaata aatcaaacca
    25741 cttaatggag gcgttttaga atggaaaaat ttcagccgat catcaatcaa atcaaagaaa
    25801 aagaacatca acaaaagatc aaagaccttt ttgctaagat aacagaaacg tatccccagc
    25861 tagacacaac tgtaaaatgg aaccaaccta tgtttactga ccatggtacc tttattattg
    25921 catttagttt tgctaaaaat catttttcta tcgcaccgga aaaagcagcg attcgtgccc
    25981 ttgaaaaaaa tatccaagaa gcaggctacg tatatacgga taacgtcatc aaagttcctt
    26041 ggaaaagtgt aatcaactgg gaattgattg aacaattgat ttcatttaat attgaagata
    26101 aaaaaggtca tgataaattt tggcgataaa gctttatacg aataaacctt ttactggcga
    26161 ggaaactatt catcacctta tacaggtttt gttcaatcct atccttttta tcatccctgg
    26221 ttaaacactt aattgacaaa gtatctgcta tttttataag gagtctttct tgtgctgaaa
    26281 aaaatcactg attgaatggc tttgaatttt ctacatagta aaattatttt ttcagcaatt
    26341 ttcctgcaaa acttttgaat aaggaaaaaa tacgagaaaa tatcagtgat ctttcataat
    26401 actgcccatt attttcaggt atatttaaat ataatatcac tttaggagag aacgaatttc
    26461 tttagagaaa taaaagttag aattttctga cttgattttt acaatgacct taattaaaat
    26521 cgttctttct acatttcgtg attaagcaaa tatcaactaa gtcagtagaa ccattcacat
    26581 aggagggtga acattgaaag atttttactc agtaaatgaa ttagccgaac aattaggtgt
    26641 tactacacgt tctatcagaa attatcttca tgaaggaaaa ctaaaaggaa caaaggttgg
    26701 cgggcaatgg aaattttctg aacgtaatct ttttgagttc ctatacggag atcaagcaga
    26761 ggaagcagca aaagaaatgc agcgattcat gctaaatgca ccaatcacga tgcgtttcaa
    26821 ccttcaatat cgtgatttta cagcaatcaa tcaattccga gaacaattag tccagtacca
    26881 taacgatgtt tacgcaaata aaaaagaccg tcttttacaa tacgatcttt ataaagacaa
    26941 tcatgcggag atactaatcg gtggtaactt caattatgtc gtaaacttta gtcaatggat
    27001 caatgaaaaa ttattgatcc agacagatat ttcccttgta tcttaaacaa ttaaaaagcg
    27061 ctagaaattc cagagatgtt ggaaattcta gcgctatttt tttataagta cttactgttt
    27121 ggttcaaaca aatatttttt taataaatga aattttacca tctttatcct caaataaaga
    27181 ccgtgaccat gcttcctact acgatcagca ataatccgat aatcgttaat tttttttctt
    27241 ttggtgtctt attttcatgc aggatgtaca taccgcctaa tgtagagata actacgttta
    27301 gctgtgtaat gatatatgca gtagcaaccc cattttcatt tgctgaaaaa atataagcca
    27361 atgctgacac actaaagatg gctccaacga tcgttgcttt ccaactcttg tcttcagaaa
    27421 atgcggttgg tttacggaaa atcacataaa tcactgcccc taaaaagaca ccgatcattt
    27481 gcggaaagaa aatggaaagt ccgcttgcat caactagttt aggcaatgca ctgtataccc
    27541 aataaccaat acttgttgat gctaatagac taattccttt tgtgataccg cctttttttc
    27601 caggatcatc actgattgcc gttaaagcag aaccaaatac aagaagaatg attgctgctg
    27661 ctccaatgat tttccccata cttccagacc attctccaaa agcgaagaca ccaatcaacg
    27721 aggttccgac taactgtaat gcagtcgaaa ttggcatcgt tttagaaaca ccaatcaagt
    27781 tgaaagatgt atactgtcct gtttgtccaa taacccaaaa tgcaccagaa agtaaactaa
    27841 tcaaaaatgt agtcactgaa ataccagctg gagaagcgaa taattgaacg agtaagccga
    27901 caagcaccgc tccaatacca gttcctaata tctcatttgt tggtctgcca ccaatctttt
    27961 tcaaaatcag tggctggatc ccccagccaa ctgccgggat caatgcaatc aataaaccca
    28021 tttttattca cccttatcct tttccacctt ggaaagaagg atataaagtc atcccaccat
    28081 ctgcaaatag tgtaatacct gtaacataac tggattcgtc tgaagccaac catgcagctg
    28141 tcgccgctat ttcttttggt tcacctacgc gtcctagtgg gatcatactt tccaagagtt
    28201 ctttttgttc aggatcatca aattttgccg cattaatagg tgtacggata gcaccagggc
    28261 ctatactatt gattctgatt cccttcggtg catactccat tgctaccgtt tcggtaaata
    28321 attttacccc gccttttgaa gcagcgtaat gagcaaacgt tggccatggg atctgttcat
    28381 gaacagaaga catattgatg atatttcctt ttttattatt ctctgtaaag taatttaaag
    28441 ccgctttcgt tccgataaaa acgccagtca aattgacttt taacactctt tcccaatctt
    28501 caaggctaag ttcatgggtt ggatgctgat tttccatccc agcattatta acccaaacgt
    28561 ccagtgtccc aaaattttct aaagccgtat caagtaaacg ctgtattcct tcttcagaac
    28621 tgacatctgc ttgtacagag actgcttttc cgcctgcctc ttcgattgct ttgactgctt
    28681 tttctgctcc ttctttatcc gagttatagt tgacaactac attcattttt tctttcccga
    28741 atcgttgagc aatcgctgtt ccaatcccct ttgagccacc agttactact gcgactttgt
    28801 tttctaattc cttgtacata gtacccactc ctttttcata cctaaacctg ttattacgga
    28861 taacttttat ttccgcctta aagtgagtgt acaccaagat atatagtcta aagcaaatga
    28921 tacgctctag caatcgtttt catgaattgc tagtgtcctt ttatttctat aaagaaagag
    28981 gttgtgacag aagtggtcag cttcaagaaa tttcggctta tttccgaaga agctacttct
    29041 gaaaaaccat ttatttggaa aaaaaggctg tgacaagact tttgtcacag cccctttcct
    29101 gggcttattt atcacccatt ttattgtaat tgctagttat acataaacaa gagattttat
    29161 gcataatgtt ttattgctga gaaaaatatt cagctgttgc ttgttcatcc tccttgagtt
    29221 gttcaatcaa ttcctctaca ctagaaaatt tcacttgatc tcttaaaaat tgattccagc
    29281 gtaccgcgac atgttcacca tagatatctt gattaaagtc caaaatatac aactctacag
    29341 tcagctcccg tccttcaccg aatgtatcat tatgtccaat tgaacccatg gctggatacc
    29401 atgtttcccc aatttttatt tccgacacat aaacaccttc tttaggcaaa tgaaccgtgc
    29461 tctttacttt gacatttgct gtagggaatc ctagtagtct gcctcgtgca tcgccatgga
    29521 caactacccc ttctacctca tatgtgcaac ctaatagttt tgctgcttct tttacttgtc
    29581 cgctgtctag taatccacga atacgggtag agctgatttt ttcaccttgt tcttcttctt
    29641 tagaaactgt gacgattttg aatcttcctt tggcatattc aggaaaatga gcgacatctg
    29701 caatgtcttt tggtccatac gtataatcaa aaccggaaac agcatatttt gcatgtaacc
    29761 ctacgatgta ttgatcaaca aattcctgcg gtgctaaatg agcaaaagaa gaagtaaaat
    29821 caacttcata taaataatct atacccaaag cggccatttt ttcttctttt tgcttaaggc
    29881 tagtcaaata tttcacgctg ctaggatcga tctttttaaa gacaatagat ggatgctgat
    29941 tgaaagtcat caatgctaat ttcaatcctt ctttttgtgc aatttccata cctgtcttta
    30001 tgactttctg gtgtccgata tgcacgccgt cgaaaaatcc cagcactaaa acgacatcgt
    30061 cttctggaat ttgttctgat tggtaaggat gtctgatctt gatgatattc attcgttatt
    30121 cctcatttct caatacttta ctaggtttta atttgttttt ttctttcggg tttggctgat
    30181 aaatactgac aacttttcct ttataaaaca aagcaatttc ttctgacggc atttctggga
    30241 gcgcaaatac agtataatca agtctcatac cgttttttac tttctgccat acttcatcag
    30301 taatatctac tcgtttgaac cgttccactc ctttttctat tggcagcaaa caatcatcga
    30361 ttgtcccatt tttcacagct tgagctactt cttctaatgt cagagcatcc ttctgggaca
    30421 taccggcact tgctgttcgg gtcagatcag acatgtgcgc agggtaacca agtttcttgc
    30481 ctgtatctac agctaatgtg cggacatagg ttcctttccc gcattctact tcgaagcgcc
    30541 aagaaatggt tcctgtcact tcgtctcttg tcaattcact tgtccggata aaacggtaaa
    30601 tctgagcctt tctaacagga cgctccactg tttcattatt gcgggcatat tcatataacc
    30661 gcttaccgtt gacttttacc gctgagtaca ttggtggtat ctgggtgatc tcaccaataa
    30721 atgaagccat cacttcatct attttttttt cagataaaga catatctact ggttttgttt
    30781 ctacccgttc tccggatcga tcttccgtcg ttgtactgaa acctagagtt atctcgccta
    30841 catagatttt cccagaatct gtcaaatatt cgataacttt tgtggctttt ccaatacaaa
    30901 tgggcaacac accgtctacg tctgggtcaa gcgtaccccc atgtccgatt tttttcgtat
    30961 gtaagatttt tcttaatttg aatacgcaat catgacttgt cattccgcgt tctttccata
    31021 aaggtaataa cccgtccatg tcttttctcc atttcatttt tgatttaact gaattattat
    31081 aacacattgc atcaattact gacgaatttg acatcataat gaacggtttt tatttttcgg
    31141 caataaaaac gtaacaaaat tcatgctcac gtaaatagaa atcatgttat gcttttttaa
    31201 aggagggaat agaatggaag attttttctt tgcaccattt gattggattt atgggtggct
    31261 tacaggtaaa tagtttggtt ttcacttccc aaaaacgaaa aaaaagactg gccattcttc
    31321 cagtcttttt tccgtttttt ttatttattt tttcagtttc ttaatcaatg taaaaaattc
    31381 tattttgcgg gatcgatttt atcattttat tggtcaagat ttttccattt ccaatccaat
    31441 acttctggca gatcaatacc attttcacga atatagtttt gatgttcttg taatttctga
    31501 tccatttttt tcgcaaaatc ttctgcttga cttcctttga tttttaaagc tgcgtctttt
    31561 gccaaatgat aacgatcaag ttcatttact acacgcatgt caaatggtgt cgtgatatct
    31621 ccattttctt tgtagccatg aatcattaaa tgatggttat gacgatcaaa gaaaatcgtg
    31681 cggaccaatg tttcataacc gtggaaagca aataagatcg gtttgtcttt tgtaaaatat
    31741 tgatcaaatt cttcatcgct caacccacgc ggatcttgac ttggatgacg taatttcaat
    31801 aaatctacga cattgatgaa ccgaattttt agttttggga attcttccag cagcaaagtt
    31861 acagctgcta atgcttctaa gttagattcc gttcctgcag cagcaatgac caattcaggt
    31921 tcctcctctt ttgctgtcga agcccaatca atgattttca agccttcatt cactaagacg
    31981 gctgcttctt ctgctgagta aaattgagca cgcggatgtt tggaagagat gatcaaattg
    32041 attttttctg acgagcggaa tgctttatcc atgactgcga gcaatgtatt tgcatccgca
    32101 ggcagatatt cacggatgaa ctccgccttc ttttctgcca aatgtgtcaa tattcctgga
    32161 tcttggtggc tatatccatt gtgatcttgc tggaagactg ttgagcttgc aatgagattt
    32221 agcgaaggat aatcatttcg ccaagaaagg tcatgtgatt tccgcatcca tttgaagtgc
    32281 tgagtcaaca tagagtcaac cactcgcaag aatgattcgt aacttgcaaa aaaccatgcc
    32341 gtccagtcag aacgtaccct tctaagaacc cttcatcttg gtgttccgat aattgactgt
    32401 cgatgacgcg accagatggc gaaagccatt catcattttc tggttcgatt ttttccatcc
    32461 attggcgatt tgtcgttttg aaaacttgat tcaatcggtt tgattttgtt tcatctggtc
    32521 caaatatacg gaaattttct ggatttttct caatgatctc gcgaacgaat ttaccaagtt
    32581 cggtcatatc ttctgcttta acggagcctg gtttttcaaa ttgcaaagta tattttttcc
    32641 aatctggcat aataagcggt cttggatcga ttccaccatt ggtgatagga ttttttgcca
    32701 ttctttggtc accagtaggc gcgatttctc tgatttcttc taaaatacgt ccttgtgcat
    32761 caaataattt ttctggttca taggatttca gccaacggag taaagcatct gcatgatcca
    32821 tatgttcttg atctacagga atcggtactt ggtgtgctcg gaaggttcct tcaattggtt
    32881 ccccgtccca ttcttttgga ccggtccatc cttttggtgt gcgtacgatc aatactggcc
    32941 attttggcat cacgacatct gttgctgtct tcaggcgagc ttctttttgg atggatttaa
    33001 ttttctcgat tgcttgatcc attttttcag ccataacagg atttaatttt tctgaatcat
    33061 ttccttcaat aaagaaaggt tcccagccaa gtccgttaaa gtaatttgct aattctccat
    33121 ctgatttacg tgccaagatc gttgggttgg ctatttttgc accatttaaa tgaaggatcg
    33181 gtaaaacagc tccatctatc actggattga taaagacatt tgagaaccag cttccagcta
    33241 aaggaccggt ttctgcttca ccatcaccaa tcacgacagc agagattact tcaggattgt
    33301 ctaatacagc tccgactgca tgtgaaagag agtatccaag ctctccgcct tcatggatag
    33361 aacctggtgt ttctggtgct gcatgagaag caatgcctcc aggaaatgaa aatcgtttga
    33421 ataatttttg cattcctgtc tcatcttccg ttacttctgg atagatctct gtgtaacttc
    33481 catctaaata agcattcgat accatgactt gtccgccgtg tcctggccct tcaatataaa
    33541 acatgttcaa atcatattta ttgatgactc ggttcaaatg gacataaata aaattttggc
    33601 caggaatcgt tccccaatga ccgattggat gttttttgac atcttcgggt ttcagtgtac
    33661 gtctcagtaa ggggttatct ttaagataaa gttgcccaac agaaagataa ttagcagcac
    33721 gccaccaagc cgtcatttta tcaaagtatt ctttactaga ataatccata cataacactc
    33781 cttttcatta ttactcttat tctagcgcag atactcccaa ttgaaaccta ataagcactt
    33841 ctgttcattt gtttggattt tatatctagc caaatgaaca ggtataaaaa accacgaaat
    33901 ttttcctttc gctgacgttt tttacattga tccaaaaaac ttgtcgttat cacaatggct
    33961 gttccgcagt tcatttcgct cctcatcatg cataatttgc tgaatgacgc tgggccagtt
    34021 aacacattgc tggaaaactg gggctggatc gagcaattcc tttcctgatt gatccgtttt
    34081 atgtaaaagt ctctgttatc tttgtcaata tgtgaattgg tatcccagct actatgctaa
    34141 tttctacagg gattatccaa aatttggatc gtgaccaaat cgaagcggcg taatcggaaa
    34201 tatcaataac ttcaacgtga tttttctttt aactgtcaac ggaccgacaa actcaaactt
    34261 ttatggtgca ggttccactg atcttctagt tacttggcta tacctgactg tcactaaaat
    34321 agattacaat ctagcttccg ttatcggtat cttgatcttt atctatatgc aaaaatttat
    34381 atactactag aaaaaagaaa gtcgaatttt ttacttttct ttttctcttt tttggttagc
    34441 tgacacgccg caagactaaa gtcatggaac tcttaggtaa tacgtcttaa tgaaaacaaa
    34501 aaaatcccgc aacccctgat ttcacaggat ttgcgggagt ttttgattag tctttgttca
    34561 agtcgcgaag caattcatcg atatggttgc catagacaac tgattcatca agttcaaaga
    34621 acaattcagg tgtcttataa atacttagat tgtgccctaa ctctcgacga atcaatcctg
    34681 atgctttatt caatccatct tgtgctgttt tcttatctga tgctaagtca gataaaatgc
    34741 tgtaatagat cgttgcttgc tgtaagtccc ccgtaacttt cacatctgtg atcgttacgt
    34801 tctccacacg agggtcacgg acctttttgc gcaaaatatc attgacttct tttaagattt
    34861 cttgaccgac tcggcggtca cggtagttag ccataatgat tggcctcctt ttatcagttt
    34921 ttagtcattt ttgacttctt ccatgataaa tccttcaatt acgtcatcta ctttgatgtc
    34981 attgaatttt tcaatcattg caccacattc ataacccatt ttgacttctt tcacgtcatc
    35041 tttgaatcgt ttcagacttg caagttggcc ttcatagatt acgattccgt cacggattac
    35101 acggacaccg ctatctctac gaatatatcc ttctgttaca taggcacctg caatcgtacc
    35161 aactttagaa actttgaagg tttcacgaac gatcatttga ccggtgattt tttcttcaaa
    35221 ttcagggtct agcatccctt tcatcgcagt ttcgatttct tcgatggctt tatagatgat
    35281 acggtgaaga cgaatatcga cttcttcagt atcagcttgc acttttgcct gcggtgtagg
    35341 acgaacgttg aaaccgatga taatagcgtt gctggctgct gctaaggtta catcactttc
    35401 attgattgct ccgacagctg aatggacgat ttttacacgg acaccttcca catcaatctt
    35461 cttcaatgat gcagccagtg cttctgctga accttgtaca tctgctttga tgattacgtt
    35521 aacttctttc aattcgcctt ctttcaggct ttcaaataag ttatccaaag tcacacggct
    35581 tgtggcagca cggttttcta ataatgcacg tttcgcgcgt tcttcacccg ctgcacgtgc
    35641 tgtcttttcg tcttcgaaga ctacgaaacg gtcacctgct tgaggtacat cattcaagcc
    35701 agtgatttct actggcgttg caggtcctgc ttctttatcg cgacggccaa gatcgtttgt
    35761 cattacacgg acacgtccgt aagtattacc gacaacgatt gggtcaccaa catgaagtgt
    35821 tccttcttgg acaagtaagg ttgacacagg acctttacct ttatcaagac gtgcttcgat
    35881 cactgttccg attgcgcgtt gtgttggatc tgcttttaaa tcttctactt ctgcgactaa
    35941 caggatcatt tccagcaatt cttcgatgtt ttggccgaat ttcgctgaga tctcaacaaa
    36001 gatcgtttct ccaccccatg cttcaggaat taactcatac tcacttaatt cttgcataac
    36061 atgttgaggg tttgcacctg gtttatcaat tttatttaca gcgacgatga ttggaacgcc
    36121 tgcagctttg gcatggttga ttgcttcaac tgtttgtggc atgacgccat catctgcagc
    36181 aacaactaag atcgtgatat ctgtgatact tgctccacgt gcacgcatac ttgtaaaggc
    36241 cgcatgtcct ggtgtatcca agaaagtgat tggtttacca tcaatatcga tctggtaggc
    36301 accgatatgc tgtgtgattc cgccggcttc gccgcttgtt acacgtgaat ggcgtaatgt
    36361 atccaataaa gttgttttcc catggtctac gtgtcccata atcgttacga ctggcggacg
    36421 tgagactaat ttatctggat cgacttcttc tgcctcgaag aatttatcga tatcagcaat
    36481 gtcaacttgg actttttctt gtggttccat gccatagtcc gtcgctaaca gttcaatcgt
    36541 atctttatcc agtgcttggt tttgattgac catgacacca agcataaata attttttaat
    36601 gatttctgct ggctcacgat gaattttttt cgcaatatct gctacgttca taccttcagt
    36661 atactctaat acatctggca attcacggaa tttgcgtggt ggtacagctg gtttagttga
    36721 ttgttgctgt ttgccttttt tacctttttt attataacga ttacggttat tattattaaa
    36781 tttgccgcga ttgttattgt ttggtttgtt gttattgtta gtacggtttt gattgccgcc
    36841 ttggctcgtt ccttgacgat tttggctgct ggtatttccc gtattatttt ggtttgttgt
    36901 tggttttttt ccttgattca cttgaccgct cccttgaccg cgatcttgat aactgcggtt
    36961 atttttattt ttttgttgat tgttcgttgt ttctttttta cgttgatcgt tcggctgttg
    37021 attggctggt tttctgcttg cttgttgtgc agcttgttgt ttcgtgcttg ggtttttgaa
    37081 cgcttgttgc aatttcttct catcattgct agtaatcgca cccatatggt ttttcacatc
    37141 gatgccaagt tgttgtgctt tttccacgac gtctttacta gattttttga gttcttttgc
    37201 taattcatag attcttttgt tgcccatgca atcaccttcc taaccttcaa ttagctcctt
    37261 ggtcttcttc gcaaatccgg catccattat tccgaatacc ttgcgaggtt ttccgatcat
    37321 ctgcgtgatt tctgcttcag aaaatagctc aaagcaagga acttcataat atgaactctt
    37381 atcttttatc ttttttcttg tattctcgct tgcatcggtt gccacaaaca ccaattttgc
    37441 tttttgtttg cggatttcag caatggtcag ttcttcaccc gtgattagtt ttcccgcgcg
    37501 catcgctaga ccgatgagat tcatggcttt ttgccgatta actgtattca ttttccaaat
    37561 aactctttac gtgctttttg gtgagtgaca tagtcaagaa gttcttgata aaactcatct
    37621 gttaatgttg tttcgagtac tcgatcgaga atttttttat cccaagcttg ttgtacttct
    37681 tccggctcaa gtgagacata cgctcctcgt ccaggcattt ttccagttgg atcgatcgat
    37741 acttcccctt cttttgaacg ggtgattcgg accatctctt ttttaggttt catttcacct
    37801 gaaaccacgg atttgcgtaa aggaattttc ctttgtttca tctctttgcc tccttactaa
    37861 agttaagctt ctggtttttc ttcgaccgtt tcattgttga atgcaattgt ttcatattca
    37921 tcgtctgtta aatctgactg aacgattgct tcatcatgca gttcttctgt ttctttttgt
    37981 tgttcttgat tttcataaaa ttccgtcatg tcagattctg atttgatatc gattttatgg
    38041 ttagtcaatt tagcagctaa acgtgcgttt tgtccgcgtt taccgattgc tagcgataat
    38101 tggtagtctg gaacgacaac tgtgcaggcc tttggatttg tctcatcgaa gatcacatcc
    38161 atgacttgtg caggattcaa tgcatttgca atgaatacag ctggatcttc attccattcg
    38221 acgatatcca tgttttcgcc ttttaattca ttgacgattg cttgaactcg ttgtcccttt
    38281 ggcccgacac atgtaccgac tgcatcgata tttggatcat ttgagcgaac tgccactttt
    38341 gaacgatcgc cagcttcacg ggcgatgctg acaatttcaa cgattccatc atagacttct
    38401 ggtacttctt gctcaaataa gcggcgcaat aggtctggat ggctgcggct aacaaatact
    38461 tgcggacctt tagacgtatt ttctactcgg gaaacataaa ctttgattcg atcatgtggt
    38521 tgataaaatt catttggcat ttgatcttgt ttagaaagaa ctgcctcaat tttaccaaga
    38581 ttgacataaa tataacgttt atcctggcgt tcgacgattc cttgcatgat gtctttttca
    38641 taagcactga attcgttata aataatcgtt ctttctgctt cacgtacacg ctgtaaaata
    38701 acttgtttag ccgtttgcgc agcgatccgc ccaaaatctt ttggtgttac ttcaaaacgg
    38761 atcgtatcac cgatctcata tgctggattg attaaaagtg catctttcaa tgagacttcc
    38821 aactgtgaat ccatcacttc ttccgttact tcttttaccg catagacatg gatctttcct
    38881 tttttctgat caaattccac ttcgacgttt tgtgcttggc catagtgacg tttgtatgca
    38941 gaaaccaatg ctgcttctaa agcatcgatc acgatttctt ttgagatccc tttttcagct
    39001 tctaaagcgt ctaacgcatt taacatttct ttactcattt tttctgattc ctctccgtga
    39061 ttaaaattgg atagctaagc gagcttttgc aatatttttt cggtcaaaag tcaattcttt
    39121 ttctcgtgtt tttatgcgta ttttcaatgt aagctgttca gcatcaaatg attgaagaaa
    39181 tccttcatat tgtttttcgc catctactgg ttggtataag gagacatgga cataatcacc
    39241 aagtgccttt tcatagtcgc tttctttttt caatggccgc tctgctcccg gggaggatac
    39301 ctccaggaaa taagcttgag gaatcggatc tggatctgct gcatcaagtt tttcactcag
    39361 tttctcactg acaaaggcgc attcttcgat atcgataccg ccttctttgt caataaatac
    39421 tcgtaagaac cagctttttc cttcttttac aaattcgact tcgacaagtt caaaattttg
    39481 ttcatctaaa atcggtgtca ccatttctgt gactgtttca acgacgctac tcaaaagttt
    39541 cgcctcctta aaaaattaag tgcgtaattc cattaaaaag agtgagcgaa aaatccgctc
    39601 actcacagtt agtatcatta cctataagaa ctataccata ctctgcttaa aattacaagc
    39661 agacaggaaa acagatgctt atagcatatc gaataacgat aattgatttt catctggtag
    39721 atctttcaat acgccattgt cgttcatata ttcgatcaat gtttttgaaa ctttaccgcg
    39781 ggtggcaaga tcttctttgg ataaaaatgg accgtctttt cttgcttcaa cgatttgttt
    39841 tgccacgttg gttcccaaac ttgggacagc tctaaatggt gcgatcaacg tatcaccttc
    39901 aataacaaag ttaacggcat ccgatttata caaatcaatc attccaaatt tgaatcctcg
    39961 ttccagcatt tcattggcta actccagcac tgtcagttga ttcttttctt ttacggaagc
    40021 atctaatcct ttgtccgtga tttctttcat tgcttgtttc acagcatctt ttcctttgca
    40081 catggagact agatcaaagt cgtctgcacg tacagaaaag tatgcacagt aatacaaaat
    40141 agggaaataa accttgaaat aagcgacacg aagagccatc aatacgtaag ctgcagcatg
    40201 ggctttcgga aacatatatt tgatttttga acaagaatcg atataccagt cgggcacatt
    40261 attttctttc atcgccgata gataggtttc tcgcaattca tcagggatct tgttccattg
    40321 tcctttacga acagtttcca tgattttaaa tgccatcccg ctatctaatc cagcatggat
    40381 caaataaacc ataatatcat cacgacagcc gattacttcc gccagtgtcg catctcctcg
    40441 tcgaatcaat tcttccgcat ttcctaacca tacgtctgta ccatgagata gtccggaaat
    40501 ttgcagtagt tctgcaaaag ttgtaggatg cgtttcttct agcatccctc gaacaaaacg
    40561 agtgccgaat tctggaatac ctaatgtccc tgttttggaa taaatttgtt cctgacttac
    40621 tccaagtact tcaggaccgg caaatatccg catcacttcc gggtcatccg tagggatcgt
    40681 ttgtggatcg atccctgata aatcttgaag catccgaata acagttgggt catcatgccc
    40741 tagaatatct aatttcaata cattatcgtg aatcgagtga aaatcaaaat gagtcgtttt
    40801 ccattccgaa ttttgatcgt ctgctggata ttggatcggc gtaaaatcat agacatccat
    40861 ataatcggga ataacgatga tcccccctgg atgctggccg gttgtccgtt tgactccagt
    40921 agcgcctttt gctaatctgt cgacttctgc acttcgaaac tgcagattat tgtccctttc
    40981 gtatcctttt acatagccat atgctgtttt gtcggcgact gtaccaatcg ttccggcacg
    41041 gtaaacatag tcttcgccaa acaatacttt cgtatagtta tgggcttctg cttgataatc
    41101 acctgagaag ttcaaatcaa tatcgggcac tttgtctcca tggaatccta aaaatgtctc
    41161 aaatggaata tcatgaccgt ctttgtttaa gcgtgcacca cattttggac attgtttttc
    41221 tggcatgtca aatccagaac catacgtacc atcttcaaaa aactcggaat attggcactc
    41281 tggacaataa taatgagggg ctaacggatt aacttcagtg attcctgtca tcgtcgcaac
    41341 aaagctagag ccaacagagc cacgtgaacc tactagatag ccatcttcat tacttttatg
    41401 cactagtttt tgggcgatca gataaataac cgagaagcca ttaccattaa tagaattcaa
    41461 ctcttttttt aatcgttttt gaatgatctc aggtaacgga tctccgtaca tttgttttgc
    41521 ttttgtgtaa ctcaattctg atatttcatc ttctgaacct ggaatcttcg gtgtataaag
    41581 ttcatctttg actggaatca cttcttcgca gatatctgcg attttattcg tattttctac
    41641 tacgatctct ttcgctgttt cttcccctaa aaagtgaaat gcagttagca tctcatcggt
    41701 tgttcggaaa tggacatccg gcaaactatg acgattcaac ggattcgctc cgcccataga
    41761 attgatcaaa atttttcggt aaatggcatc ttcttcgttc agataatgta cattacctgt
    41821 tgcaacaacg attttatcca aactttttcc gatttctacc aaattttgga taatttcttc
    41881 caaatcatgt tcatttttta ccaattcttg ctcgatcaaa ggcgcataga ccgcttttgg
    41941 cataacttca atataatcgt agaattttgc tcggtttctt gcctcttcta cccctttttg
    42001 catcatggct tcgaatattt ctcccttatc acaagctgag ccgatcaata gattttcacg
    42061 catctttttc aattgggagc gtggtattcg tgggacgcgt tcgaagtatt cgacattcga
    42121 cattgaaatc agtttgaaca aatctttcag tcctgcctga ttttttgcta aaatcgttac
    42181 atggaaaggt cgagcccttt tgtaagaatc tccttctccg atatgctcat ttagctgatc
    42241 atgataaagc atgttatgat tgtccattgc ctctttcaca aagatccatg ctaagtgacc
    42301 agttgcttca gcatcataga ttgctcggtg atgctgttcc aagctgacac caaatttttt
    42361 agataacaca cctaatccga aacgtttgaa ttgaggatac agatatctag ctaattccaa
    42421 cgtatcaatg actggattgg ccgcttcagg aataccatac cttgcgtagc tagtattcaa
    42481 gaaccccata tcaaaagccg cgttgtgggc taccaaaatt gtatcttttg aaaattcaag
    42541 gaacatccgc aatacttctt cttccgactt tgatcctcga accattccat ccgtgatacc
    42601 agtcagatct accgttgttc gtgacaaagg atgtcctgga tcgataaatt catcaaagct
    42661 ttcgatcaca tttcctttat acattttgac tgcggctagc tcgatgatcg tgtcatatac
    42721 tgcagacaat cctgtcgttt ccacgtcaaa tacgacatat gtggcttcac tcaatgcttc
    42781 atgtgcatca ttataagcaa ttgggacacc atcatctacg acattcgctt ccacgccgta
    42841 taagattttc acacctgctt tttttccagc actatgtgct tccggaaagg cttgcgcacc
    42901 accgtggtct gtaatggcga tagctcggtg tccccatttc cctgcttgtg ccacaagatc
    42961 agagatgctg tttgttgcat ccatcgtact catattgcta tgaacatgaa gctctactcg
    43021 tttttcccct tcaggagcat aatctttgcg aggtgtatgt ttgacttcta tgatatcttg
    43081 cgcattcatg accaaatcac ggacaaacgt atcttcttga atacttcctc gtacctttaa
    43141 ccagctaccg acactgatcg catcaaatac ttgttcatct ttttcgccgt tcgagaattt
    43201 tttgacgata aatgatgaag tataatctgt gattttcaaa gtcaagatct tgcgttttga
    43261 acgtagttct ctgacttctt tgtcgaatac gtacccctca agtgtgactc ttcgttcttc
    43321 ttcaacgata ctgatcattg gtgtcgttgg ttcatcggct ggaatatttc gtcctagctg
    43381 gatgtggatg ggtccttcaa gagctggcga ttgctctttt ctttcctttt tcttttgttc
    43441 gtggacaact agactttcag ctgcttgctt cataaaggct tctgcctgtt cttgtttgcg
    43501 ttcttcaaaa agtttcaata ctcgttccgc ttgctgctca tccacttctg gttcgatacg
    43561 aaattttggg aatccgtaac tgacgaacaa ctcttctact aatggcaagt attgttgctt
    43621 caaataagaa atagctcctg tactgtcgat tggcaagatg actttacgat cttttatcac
    43681 aggtacctgc gttttcaata cttgctgtgc tagtggtgta tcgcattgct gattttccaa
    43741 agcttgggcc cagtaatctt gcaagagctg ttcatcgaat gtctggtcat ctgctaagat
    43801 attcagtttg acttgtgcaa tatctttaaa agcgatagtc agatgctgag tcaatgaccg
    43861 atatagcata ataggcaaaa ttttggaaaa atgcagcgta aattcccata aacgcgattt
    43921 acgatggacc actacactat ccatttcacc agcagtaagc agtggatgat ttttttctgc
    43981 ttcctctagt tggatttgtt ccaacagttt ttcaaataaa tctcgttttt ctgacaaaga
    44041 tatacgccct ttctgatgag aaacaatgca attgatcgca tcttatcctc ttctttttca
    44101 gtacctttta gtatactcgt tttttctgag ttttacatta aaaaattgga actgcgattc
    44161 aaatcgcagt tccaatttta aaagcgaatc atcagattgt caaaaacagc tgattcagct
    44221 atttttattt tctgtgtttt aacgcttatt attcgccttg gttcaagagg attggtaaag
    44281 tattcaccaa ttcttcttta cgtacttcaa ccatttcgcc agttttcttg attttcactt
    44341 ctacgattcc atccactgct tttttaccga cagtgattct gattggacag ccaatcaaat
    44401 cagaatccgc gaatttcact cctgcgcgtt catttctgtc atcaaccagt acttcatagc
    44461 cagcttttgt catcgcttct tctagttcat tcgtcaggtt tgtttggtat tcgtctttta
    44521 gattcatttg gacgatatgc aaatcaaatg gcgcaattcc ttttggccag ttgattcctt
    44581 gttcatctga atgttgttct acgattgctg ataataaacg acttacaccg ataccataac
    44641 agcccataat cacaaatttt tctcgtccat tttcgtctag tacagttgct cccatagatt
    44701 cactgtaacg agtgccgatt ttgaagatat gtccgatttc gattcctcga gtaaaggcaa
    44761 gaacaccatt gttgtctggt gaaggatctc cttcttgtac aaaacgaaga tcttcataag
    44821 aaagtacagt gaaatcgcgt tctggattca catttttata gtggaatcct gtttcgttcg
    44881 ctccagtaat ggcattcgct aaatcttgga cataacggtc tgcatatact tttacgtcat
    44941 ctgaaacacc gactggtccg acagaaccaa actcagcacc tagatatttt tgtgcttctt
    45001 cttctgttgc ttcttctaaa aagtcagcag ataaaaagtt tttcagtttt acgtcattga
    45061 cttcatggtc tccgcgtacc aaaacaagga caggttgttc atccgcaatg aacaatacag
    45121 acttgatgat tttttgaggt tcaacagaaa agaaatctgc tacttcgtca attgatttta
    45181 cgttaggtgt agctactttc tccaattcaa gctgtgtttc atgcgatttt tttgatacat
    45241 agtagcttgt agccatctcc aagtttgcag cataatcact ttcagtcgaa taacagatcg
    45301 tatcctcccc gatttctgag atcgccatga attcttttga gtctttcccg cccattgcgc
    45361 caccgtctcc gataatcgag cgaaattcta agccacaacg cttgaatact tcggtgtagg
    45421 ctttttcgta atctttatat gtctcgtcca aactttcttc cgttgcatgg aaagaatacg
    45481 catctttcat gataaactct cttccgcgaa gcaaaccgaa gcgaggtcgt ttttcgtcac
    45541 gatatttagg ttggatttga taaagattta aaggtaattt cttatatgaa ttgatttcat
    45601 tacgcatcaa atcagtaaaa gtttcttcgt gagttggtcc aaggatcatt tttcgatcat
    45661 tgcggtcttt gaaacgatac aagtttggtc catatgtttc gtaacgcccg gattcttccc
    45721 aaagttctgc tggcaagatg gcaggcatca gcatttctac cgctccgatt ttttcaaatt
    45781 cttcccgcat aatgcgtttc agtttttcca atacacgatt tgctaatggc agatatgaat
    45841 aaatacccgc tgatacttgg cggatatatc cagctcttag caagatttga tggcttaata
    45901 cttctgcatc atttggaact tcgcgtaatg ttggaatcaa catttttgat tgtctcattc
    45961 tatatactcc tttatttcct caaaatagtt atcgtttgtt attataccct atttgtcaac
    46021 tttttctaga aaaagaatcg ttgaatatcg ttccatgtaa ctagcaccat caacaacatt
    46081 aaaaatccaa agccagctaa tgtcaatatt ccttcttttt cttgacttaa tggtttcccg
    46141 cgaatccctt caaaaatatt caagactaat ttcccgccat ctaaggcagg aatcggaagc
    46201 agattgacga ttccgagatt cattgaaaga agcgccatca aactaataac agttgttaca
    46261 ccttgatttg ctgcttctga agacaattgg tacatcatga caggtccgcc taatttatct
    46321 aaactgaaac ctgtaaatag tgatcctaag gctttgaaaa tttccaatga accgctgaaa
    46381 gcttgacgcg tccctccaat gatcttatcc ataaaacctg tattcattgg ggcttttata
    46441 ccaagttggc caactttttc gccatttgac tctacagatt tcggtgtcac gtcgacagat
    46501 tgcacctgac cttctcgttc gatcttaaag tctaatgttt tatccggatt tttcgtaatg
    46561 actgttgtca gatcattcca tgaatggatt tcttttccgt ccacactgac tacctcatca
    46621 ttttctttta agcctgcttc tgccgcagct ccattaggca tgatctctcc tacgcgatta
    46681 gtgttcgtca cttgaacacc accttgcata aatgccagaa tgatgaataa caggattgcc
    46741 aaaataaagt tattcatggg tccagcaaag ttggtcagca tacgctgcca caatttagcc
    46801 gattggaact gtacgtcttt tggcgcaatt ctgatttctg ttccgtcgtt ttcaataatc
    46861 gtcgcatcgt gatcaacagg gtaggttact acttctgttt cgtctccatt cacataacca
    46921 gtgatagtca gctcatcttc taaatcataa cggctcaatt ccatcgggat agcatttgtc
    46981 aattggattt tcttacttaa atttatcttc tctacgatgc cgtcagaatt tagtaaaaga
    47041 gaaagaggca ttcctggtgc catttctgtt tcatcatccc cattgcctgc cattcgaaca
    47101 taaccgccaa taggtaatag gcgaagcgta tatgttgtcc cgtcttttgc ttgatgccca
    47161 taaattttcg gacccatacc gatagcaaat tcgcgaacga ggattcctga tcgtttcgca
    47221 aagaagaaat gaccaaactc atgaacaatc actaatatac caaaaacgat gataaatgtc
    47281 agaatcgttt tcatagcttt tttctccttt acttgttttt tgtgtttttt ataaaacaac
    47341 gataaaacac tataatttta ccatagtata ccaaagacac caatagtatc aatataatga
    47401 aaaataaaaa cctgcgtctt ttatgaaatt cacaaagaaa cgcaggtata cttactaaat
    47461 aatgccaaat aaatgcatga taggaaatac aaacagcaaa ctgtcgaaac ggtctaagat
    47521 tccaccatgg cctggtaaga tatttcccga gtctttcaca tcataatgac gtttgattgc
    47581 agattcgacc aaatcaccaa attgtccgac tgctgaaaaa ataatcgtca tcagaagcat
    47641 gacaaacata ccatgattaa atagttcttt tgctgggtat agcaataaat aaagaaatgc
    47701 gactacaaca gcactaccaa taccaccgag cgcaccttca atcgttttgt tgggcgaaat
    47761 atcaggactt aacttatgct gaccataacg tcgaccaacc atatatgctc cgatatcagt
    47821 tgcccaaaca ataaatagac caaatatcaa aacagccaag ctttctgttc gtgcattgac
    47881 aaaattctgg aaacctattc ccacatacaa gctgacaagc acaggaaaac ctgcttcatc
    47941 aatcgtatac gtatttttag aaataactga tgttcctaaa agaatcataa ctataagata
    48001 aaataacata aatgtactta cattctctgg aagaaagaaa aaccatcttt cttttggcaa
    48061 aacaagtaac actgcaccaa cggcggataa aactccttca aaacttaaga gtgtcaatcc
    48121 tttcatccga aacagctcgt aaactcctat tactgctaag attgctgctg taagctcaac
    48181 cgctatgcct ccgatccata taatcggaat aaacaagact agcgccacgg ctgctgtgac
    48241 cacacgttgt ttcatgactg atcccctttc tcatcttctg ttttttttac accgccgaat
    48301 cgacggtctc gattttgata agaggcgatt gcttcttcaa gatgcgcacc atcaaaatca
    48361 ggccaaagcg cttttgtaaa atacagctca ctatatgcga tctgccataa cagaaaatta
    48421 ctaattcgct cttctccact tgtacggatc atcagctcag gatcttgtaa ctctttaggc
    48481 aagaaacctg tcattaaatg cttagcaatc aattcatcat cgatttcttc tgcgtgtatt
    48541 tctccttttg ctacttcttc ggcaatttct tggacagcag tcacgatctc tgctctgctt
    48601 ccataattca aagcaaaatt caataccatt ccagtattgt tttttgtttg ctcaattgca
    48661 cgtcttacag catcttgcgt atgttctggc aatacgtttt cataacccat gacatgaact
    48721 ttgacatttt ctttaatcaa ttctggtacg aacgtatcaa agaagtcgac aggtagttgc
    48781 atgagaaaac ttacttcatc ttttggtcgc ttccagtttt cagtcgaaaa ggcataaagc
    48841 gtcaacactt tcactccaag ccttgatgct ttttttgtga ctttctttac tgtttccatg
    48901 ccttctttat ggccagcaac tcgtggcaaa cgacggtttt gcgcccaacg accattgccg
    48961 tccatgatga tggcaatgtg ctgaggaatt ggtccatcgg tattaaattg aaattcgctt
    49021 ttttcttgaa tatatttgtt tttttgcgga aaaaaacgta acattctttt tcctcctata
    49081 cttaaaagat tcccactatc ttaagtatta tagcaatatc taactgtttt tcctatgaaa
    49141 tcattgtttt tttatttaaa aactgaataa ttttttcttt ttttaatttt taaataaaag
    49201 aaatataaat aaaaacagag gaagcggaat gatccgcccc tctgttttta tgattttatt
    49261 aaacttccaa taattctttt tctttatcag cagcgatctt atctacttct ttgatgctgt
    49321 tatctgtcaa tgtttgtacg tctttttcta atccgcgcaa atcatcttcg gtaatatcac
    49381 catttttttg ttgtttttta tattcatcga tcgcatcacg acggatgtta cgaatcgcga
    49441 tttttgcatt ttctgcttct tttttaacat ctttagctaa ttcttttctg cgttcttctg
    49501 tcagttgcgg gataactaag cgaatgacat ttccatcatt ggttggactg attccgatat
    49561 cacttgccat aatagctttt tcaacatctt gaagaatgct cttgtcaaaa ggtgtgatca
    49621 ttaaaacacg tgcttctgga atttggattg atgccatttg gttcaatggt gttggcgcac
    49681 cgtagtagtt cacagtaata cggtccaaaa ggctagcgtt cgcacggcct gcgcggattt
    49741 gcccaagttc acgttgaaga ttttgtgctg cttttttcat tttttctttt gcttctgtca
    49801 taattgcatc tgccattttt atttccccct tacagttgtt ccgatatttt cgcctaagat
    49861 tgcacggcga atatttccgt gttcatttaa attgaagaca agcagtggaa tatcattatc
    49921 catgcttagt gaacttgcag ttgaatccat gacttgtaat cctttggcaa taacttccag
    49981 atgagtcagt tcttcaaatt ttactgcatt tgcatctact ttcggatcag cagaataaac
    50041 accgtctaca ttgtttttag ccatcaggat aacatctgcg ccgatttctg ccgcgcgtaa
    50101 tgctgctgtt gtgtctgtcg agaagtacgg gttgccggta ccgcctgcaa agataacgat
    50161 ccgtcctttt tctaaatgac gttctgcttt acggcgaata tatggctctg cgatttgacg
    50221 catttcaatc gaagtttgca cgcgtgtcgg tacacccacg ttttccaacg tatcctgtaa
    50281 agctaaggca ttcataactg tagctaacat acccatgtaa tcagcttgag cacgttccat
    50341 gcccatttgt gctccgattt gtccgcgcca gatatttccg ccgcccacaa cgattgccat
    50401 ttcaacgccc agctcgtgaa cttctttgat ttcttggatg atttctttga ttactggtgg
    50461 tttgattcca aatcctgcgt ccccagctaa agcttctcca ctcaatttta agactacacg
    50521 ctgatatttc ggtgtgacca tgttcattcc tccaatattt tctattgcca ttttaccata
    50581 cttagatgat ttcgacactc taagactggt ttttcttgtt ttgcttaaaa aaagggagcg
    50641 cacaaaccgt gcgttcccgt ctgttattca acagaattat tttttcactt ggctcataac
    50701 ttcttctaca aagttatctt cgcgtttttc gataccttcg ccaacttcga aacgtacgaa
    50761 tgttttaaca gttgcgcctt ttgatgcaac gtatttttca acagtcatat caggatcttt
    50821 aacgaatggt tggtccacca aagcgatttc tgctttgaat ttcttcaagc gtccttctac
    50881 cattttttca acgatattcg ctggtttgcc ttcgttcaat gcttgttctg taagaacagt
    50941 tttttcatgt tctaattcag cttctggaat ttgtgattcg tttacataac gaggattgat
    51001 agctgctacg tgcatagcta cgtctcttgc aactgtttcg tcagttgttc catctagaac
    51061 agtcaatacg gcaatacgtc cgcccatatg caagtatcca ccaaatgcag cattgtcgtc
    51121 tttttctaca acttcaaagc gacggaagct gattttttct ccgataactt gagttgcttc
    51181 gatcaagtca gattcaattg tacctttatc tgttttgatt ttcattgcag cttccatgtc
    51241 tgctggtttg ttttcagcaa caagttctgc aatttctttt actaaatctt ggaacatttc
    51301 gtttttagaa acaaagtctg tttctgagtt tacttcaacg attgctgcag tgttaccttt
    51361 cacagcaact gaagccaatc cttcagctgc aatgcggtca tttttcttcg cagcctttgc
    51421 cattcctttt tcacgtaaaa gatcaactgc tttttccatg tcgccttcta cttcaactaa
    51481 tgcttttttc gcatccatca ttcctacgcc agtcatttcg cgtagttctt ttaccatttt
    51541 agctgtaacg tctgccatgt ttaactcctc ctgtgtgttt ttctttttaa aaaaagctgc
    51601 ttcaaaggag agtgaggctt gtcgcccagc ctttgaaaca gctccatcat tcaaaattat
    51661 tctgctgaag aattgtcgcc ttcaacaaca tctacgattt cttcgattga tgttgctgtg
    51721 ttttcttctg tgaaatcttc ttcaactact tgatcttcac cttggtttcc ttcgatgaaa
    51781 gcatcagcca tttttgaagt aatcaattta acggcacgaa ttgcatcgtc gtttgaaggg
    51841 attactacgt cgatttcatc tggatcgcag tttgtatcaa ccatagctac gattgggata
    51901 tttaatttgt gtgcttcttg aacagcaata cgttctttac gtgggtcaac gatgtacatt
    51961 acatctggga ttcttggcat atcagcgata ccgcctaaga atttttcaag acgttcacgt
    52021 tctttgttca aaccagcaac ttctttttta ggtagaactt caaaagttcc atcttcttcc
    52081 attgcattga tttgtttcaa acggctgata cgtttttgga tagtatccca gtttgtcaat
    52141 gttccgccta accaacgatg gttaacgaag tattgtcctg cacgagttgc ttcttctttg
    52201 attgcttctt gtgcttgttt ttttgtacct acgaataatg ctacgccgcc ttcttcagca
    52261 acgctcttca tgtaatcata agctgcatct actaatttaa ctgttttttg caagtcaatg
    52321 atgtagattc cgtttctttc tgtgaagata tatttcttca tttttggatt ccagcgacga
    52381 gtttggtgac caaagtgtac gccggcttct agtaattgtt tcatagaaat tactgccatg
    52441 tttgtttcct ccgatttggt tttatttttc ctcttcttat cttctacctt gccggcaaac
    52501 tttttcaagc acctaccgac aatccaataa gaatgtggat ttgctgatgc ataaagcacc
    52561 ctcagctatt atacagacaa aagatccgta tcgcaagagc aaatatactt ttctgctcaa
    52621 cattcaatag tttacctgaa agataaaaag aattcaagaa aaacagtaga tttctctttg
    52681 tttctttatt atcctttttc cataaaataa ttttaggtat aaaagacgaa tattattggg
    52741 caatccttct tttttttgtt atgatacatg tgaaatcttt cgtaaaggag aaagaaaatg
    52801 cgcaaagctc aaagacattt gctgattaaa caagtcattg aagaattccc gatccgtacg
    52861 caagaagaac tattaaacaa acttgggcat tatggcgtta ccgccacgca ggcaacgatt
    52921 tctcgagata tccgtgatct aaaaatcgtg aaggcacctg atgaaaatgg cgtttcccgc
    52981 ttcgtattgt ttcgcgaaaa aggaactcct gagactagga ctgaggaaga ggatcggctg
    53041 gttcaaatga ttgaagacat tgtattgaaa gtcgaacgag tccacttttt gacgatcgtc
    53101 aataccttgc cagacaatgc tcatttattt gcatctgttt tagatgagat caaacctccc
    53161 tctattgtca gtacaattgc tggattcgat acgatcatgg tcatctcgga aaatgaggag
    53221 gacgcaaaac gagtagaaga atttcttcac catccaactg aagtatcact tttttaatca
    53281 gagcacgtat tcctaatatg gaatgcgtgt ttttttattt tcaatatttt ccttattgaa
    53341 taaaccttta cactctttac aaaaatacat cattttttat tcaaattttt caacaccttg
    53401 attgaatcac aataatcatc atttatattg gatgttaaca agagcagctt cttgctcttt
    53461 gtgtatttca aaaaagaaat gaaaggaaat catatgtata aattattaac ttctggacca
    53521 ttgacgactt ctgaaacagt gaaaaatgct atgctgatcg atcattgtac ttgggatcaa
    53581 gaatacaaag aaattacaca atggataaga aaaaaattat tggatcttgc tcaagtatcg
    53641 gaaggaatat acacaacggt cttaatgcaa ggtagcagct ccttcggggt agaatcggta
    53701 ttaagttctg tgatcaggcc agaagatact gtactgattt gttcaaacgg cgcatataga
    53761 caacgaatga tcaacatgtg cgaatgtctg aaactgaact ataccgttta tgaggtatcg
    53821 gaacatcaaa tcctccaagc tcatgtgatt acacagctga ttgaagagaa tccgtctatc
    53881 tccgcactag caatggtcca tagcgagact acttccggta ttttgaataa tttggaagag
    53941 attgctgctg tcacacaaga aagagatctt ttgtttatag tggacgcgat gtcaagcttt
    54001 ggtgggatac cgatttgtgt agccgaacta gggattgatt ttttgatttc cagctcaaac
    54061 aaatgtattc aaggtgttcc aggcttttct tttgtacttg ctaataaagc ggaactggaa
    54121 aaacgaaaaa atgttagccg aagtttgtca cttgatctat atgaccagta ccgcgtgatg
    54181 gaaaacgaga atggcaaatg gcgtttcact tcccctaccc atacggtttt ggcttttcaa
    54241 caagcgctga aggaactaga ggcagaagga ggaatcgaaa ctcgatattc acgttatctc
    54301 gccaataatc atgtgctacg acaaaaaatg acagagatgg ggttccagcc tttcattcaa
    54361 gaatatcagg ggccattcat cacctctttt ctttacccta aggactggcg atttgatttt
    54421 aatcattttt atcagtattt aaagcaaaat ggatttgtga tctatcccgg gaaaatcagc
    54481 gatcatgaat gttttcgtat cggaaatatt ggcgagatct atccggaaga tattttggaa
    54541 ctgacagcaa ttatcaaaga ctataaggag aacgttaatg attaaaactg taatttttga
    54601 ttgggcagga acaactgtgg actttggctg tatggctcct gtccacgctt ttagaaatgc
    54661 ttttctagaa aaaggaatcc aactgactga caaagaaatc cgctaaccga tgggaaaatt
    54721 aaaatgggat catatccaac agcttctttc cctcccttct gtcaaaaaac aatggatagc
    54781 tatttatgga caactacctc aggaaaaaga ggtggaggaa ttgtatcaac aatttgaaca
    54841 ttatctgttt aaagatttgg tcaagcattc taccttgaaa cccgacacat tggaaacgat
    54901 cgaacggttg agaaacgctg ggatcaatat tggttccacg aatggattta atgctcctat
    54961 gatgacaatc gtagcagaaa ccgcaaaaaa agaaaggtat tcaccagact tcatggcaac
    55021 ttttgaagac gtcgaagggt atggtcgacc gtttccttat atgatccata aaaacatgca
    55081 gtacttcaaa aacaagtcgg ttgatgaagt aatcaaagtc ggagatacgg tctcagatat
    55141 ccaggaaggg aaaaatgccg gagtgttgac agtaggtgta attgaaggta gttcgctaat
    55201 ggggatttct tatgatgagt accaaaaatt agagatgggg aaaaagatcg tttaaaacag
    55261 tctgttgctc ttagattcca acaagccggc gctgatattt gtgtaaacaa tttaaaagag
    55321 ttaacagaat acattttgta cgctgacatg aatccagaat gacttttata caaaataaga
    55381 ggttgtgaaa aagctgtcct gtatcaagta ataaaaacag ccaagcaaaa atagctcctc
    55441 atatttttcg tttggttagg cttattaccg cagatgcaag cttttgaaca ccgtttattc
    55501 gaaaaaaaaa agggagctgt gacacgactt ttgtcacagt ccccttattt gcaaacaaac
    55561 agtgtttaag agacaacctc tccactaaac ggtgttcatt cagctgactc tcggtatttg
    55621 ctcaaaaaac gataaaattt gaaaagctat tttctctttt gtttctctaa tactcggatc
    55681 agaacgctga tttcacattc tctttttctt acatcttatc tggtgcatga agtcctagca
    55741 aacgaaggtc ttctttcaat aatacagtca ctgcataaac tagagctaag cgtgcttctt
    55801 tttgttcatc atcagctaaa atcttcgtat gagcatagta tttgttgaat gcttgtgcta
    55861 atttgattgc atgtttcgca atcactgacg gttcgtattt ttccccagca gacaagaccg
    55921 tttcagggta tttttgaact aatttaacca cttcccagct ggtatcgtca ttcaatgcat
    55981 agtctgctgt ttctgaaggg acaaatcctg ctttttccaa caggctgact gcacgagcat
    56041 gtgtatattg cacataaggt ccagtttctc cttcaaatcg aacaacttct tccaggttaa
    56101 agtcaaatgt attcagtcga tcgtttttca aatcgtggaa aatgactgcc ccaaccccaa
    56161 cttgtttagc tacagcatct ttgttttcca aatcagggtt tttctcgctg atttgttctt
    56221 tagcagattg gattgcttca tttaatactt cttctaataa gacgatcttt cctttacgag
    56281 ttgataattt tttaccacct tgcgtgatca aaccaaatgg aatatggtgc atgtcgtcag
    56341 accagtcaaa gcccatttcc ttcaagacag ctttcaactg tttaaaatga tagctttgtt
    56401 cattccctac tacatataga gattgtttga agtcgtaagt acgtttacgg tacaatgcag
    56461 cagctaagtc acgtgtaatg tatagtgtcg caccgtctga cttcttaatc aacgctggat
    56521 tcaggtcata agcagatagg tcaacgatct ctgcaccttt atcctcgttc agcaaatgtt
    56581 tttcttctaa aagtttgacg atttcatcca tcttgtcatt gtagaaagct tcaccgttca
    56641 acgaatcgaa acgtacttcc agcagatcat agattttatt gaattctttc atcgattcat
    56701 cacggaacca ttgccataat tgaatagcct ctttatcgcc ttcttccaga cgtttgaacc
    56761 aagcacgagc ttcttcattt aattctggtt ctgtttcagc tacttcatgg aattggacgt
    56821 ataaacgaag caattcattg attggttctg ctttaacagc ttcttctgta ccccattttt
    56881 tgtaagcgac gatcagtttt ccaaactgtg ttccccagtc acctaaatgg ttgatgcgga
    56941 ttggctgata accgattttt tccataatga aaccgatcga attaccgata actgtcgaac
    57001 gcaaatgtcc cattgagatt ggtttggcaa tattaggaga agacatgtcg attggtactg
    57061 ttccttgatt cccgatgttg ctatccccat aatgttcttt ttctttcact actgtttgca
    57121 aaactttttt gctgataagt tctttattca tgaaaaagtt caagtaaggt ccgacaactt
    57181 cgattttttc aaagttggca gaatcgattt tttctgctaa atctgcagcg atttgctgtg
    57241 gcgcttttcg gtaaactttt gctaatgaaa aggctggaaa tgcaacatct ccgtgttcag
    57301 ctgattttgg attttctagt aattgctcga cttgttccag tgttaaatca tctttgacag
    57361 cgtcatagac agcttttgcg acaagatctt tattattcat ctttattcct cctaaagtgt
    57421 tcccttgatt gttaaacaaa aaaaatctct agcgacggca tacttagtat gccatcacta
    57481 gagacgagat ttcccgcggt accactccaa ttgtcctaaa aaaggaccag ctccatctga
    57541 tatcggtaga cgaccgtctg tttaaaacag aattctccaa gtgcgcttcg ttgtatcttc
    57601 ttcattcggc ttccaccact tccgaattcg ctagccaaga atgatacaag tactctcttg
    57661 ttcaacaaat cttagtctta ttaaactttc ttgaattata tcagaaaaaa aaacgaaagg
    57721 ctagtcgttt cataaaaaaa caatccagat ggcctaaagg agaagaaata ccacctggat
    57781 agctataaat catttcgtaa atacgaaaga cttaacaaat taatcagcta cgataatcgt
    57841 tccttcttca gaagctaaaa gattctcgat attttctaaa gaagtgatga ttgccttagc
    57901 gtttggtttt gcttcaacaa atgcaatagc tgcttctact ttcggaagca tgcttcctgg
    57961 agcgaattgc ttttcatcaa tgtattgttt catttctgaa acagtaacag tttctaattt
    58021 cttttgatct ggtttttggt aattgacata cacattgtct actcctgtca atacgatcag
    58081 caaatccgca tcgatgattt cggctaattt ttctgaagca aaatctttat cgattacggc
    58141 ttctcgtcct tctaatccag tagctgtttc tacaacagga atcccaccgc caccaacaga
    58201 gactgtgata actccttgat cgaccagcgt ttcgatgaca cgtgcttctt taatcgagat
    58261 gggtttaggg gaagcgacta cttttctcca tccacgtcca gcatcttctt tgaatgtcac
    58321 agtggagtca gcattcattt gttctttggc ttcttcttcc gtgtagaatg gaccgactgg
    58381 tttacttgga tttttgaatg aaggatcatt ttcatccaca ataacttgag tgactaaaga
    58441 aaccacatct ttatcgatcc ctttttcctt cagtacttcg cccatggcgt tttgtaacca
    58501 gtaaccgata gatccttctg tcattgcaac acatgtatct aaaggcattg ccggtgtttt
    58561 ctcagaatca gcagcttgct gctgaatcaa taggttaccg acttgcggac catttccgtg
    58621 ggagatgatc aattcatctc cttgttcgat aaatttcact aaatactttg ctgtttccat
    58681 taacgcttct tgttgggctt ttgcacttgc atccgtagac aagatggcat tgccccctaa
    58741 agcgacaact acttttcgtt tggccatttc attcgtcctt tcttcagttc taccgaattt
    58801 tctagataac aaaacgagcc agcgttttgt gattttccct caatgctgac tcgttttttc
    58861 gtgagatgaa tgtggttatg cttaaacgcg agggataaac aagttaccta atgttgctgc
    58921 catgatcgct ttgattgagt gcatacgatt ttctgcttgg tcaaattgac gagcatattt
    58981 gctgcggaag acttcatctg ttacttccat ttcagtgatg ccaaagcgtt ctttcatttg
    59041 ttcaccgtaa acagtgtttg tatcatggaa agctggcaag cagtgcaaga agatcaaacg
    59101 atctgtgttg tgtgtttttt ctaccatttc catattgatt tggtaaggtt tcaacaattt
    59161 gatacgttct tcgaatttat cttcttcacc cattgatacc catacatcag aataaaggac
    59221 gtctgctccg tctacacctt tatcaacatc gtcggtaatc atcaattgtg caccggattt
    59281 ttcagcaaag ccttctgcca ttttgacgat ttcttcttca ggttgtaatt cttttggtgc
    59341 aacgatacgc atattcgctc ctaaaattgc acctgtgact aataaagagt tagccatatt
    59401 gtttcgacca tctccgcagt aagcaacagt gataccttct actgttccaa agttttcttg
    59461 gatagttaaa aagtcagcga tcatttgtgt tggatgccat tcatcagtca agccattcca
    59521 aactggaaca ccagagaatt ctgctagttc ctctaccatt ttttgactga atccacggaa
    59581 ttcgattccg tcaaacatac gtcctagtac tttagctgta tcttctgtgg attctttttt
    59641 acctaattgg atatcatttg cacctagata ttcaggatgt gcacctagat cgatcgctgc
    59701 agtagtaaat gcagaacgag tacgtgtaga tgttttttca aataaaagcg caatattttt
    59761 tccttctaga tagtgatgtg ggataccacg ttttttcaag tctttcaagt gttctgaaaa
    59821 atcgatcaaa tattgtaatt cttcttttgt aaagtctttt tctgctaata agcttcttcc
    59881 ttggaatact gattctttca tattaaacat tctcctttag attgttttta tttaagatta
    59941 aagaggaaat cccgacggca gctgacagta agactgccgt caggaaacaa attacttcaa
    60001 gtcttcacgg acaagtggtt ggctcatgca gcgtggacca ccacgacctc gtgacaattc
    60061 acttgaattg atttctaaca ctttgatgcc atagctacga agcaattcgt tcgatacata
    60121 atttcggtta taagtgacta caacacctgg agcgattgcc aatgtgtttg atccatcatt
    60181 ccattgttct cgaggtgcaa cgatctcatc tccattacca gttgggatca gtacaagatc
    60241 atctaatcca agagcagctt tcatcgtttc gtgtaagtta tctgaatgag taatcttgat
    60301 atcatcacca tctggttcga ttgtaaagac atccatctta ccgtctttac tttgaattgc
    60361 tggatggata gtgaatttat cgtgatctac cattgtaaat acggtatcca aatgcatcat
    60421 tgcacggttg ttcggaattt tgattgccaa cactttttca aatcctgaat tttttgcgaa
    60481 taggtggcgt gctaattttt ctaaagcttt cgcattagtt cgttgagaaa tcccaacagc
    60541 tactacttta tcgcttaaga tcaattcgtc tccgccttcg atatgatctg gattttcacg
    60601 atccaaccat acttcgattc ctttatttgc aaatcttgga tgatgtttca aaatatattc
    60661 tgtaaacatc gattcacgac gacgtgcttc aaaagtcatc ttattgactg tcatcccatt
    60721 tcccattgat gcggaagggt cgcgtgtaaa gtacaagttt ggcattgggt ccatgtaaaa
    60781 tggatagtca tcgtctgctg acagatcata tagactgcta gaacgtactt cgatatcttt
    60841 tgtacgaacc ccagccatga ttttatctac catttcttgc gtttccattg atagaaggaa
    60901 ctcatgaaga ccctctctta cagcattcga tgcaatatga gattcatcca acattttatt
    60961 caagaattgt tctttgacat tgcctgcgtc aatcgcttct gccgctaatt tttccaagta
    61021 aagagtctcg acgccttcat tttgcaatgt cttcgcaaag ttgtcatgtt cttcttgagc
    61081 aatcggaaga tatggaatat catcgaacaa cagacgatcc ataatatctg gtgtcaaatt
    61141 ttccacttct tgtccaggcc gtttcagtaa aactgtcttt aatttcccta tttcagagaa
    61201 aacgtgaata ggtttatcca tgttgtgttg cctccttctt tttgattaca tgtataggat
    61261 atcctccttt tgtaatcact ttcataattt tgtctcattc aaaatgagac attcctcaca
    61321 ttaatattcg ataattaaat aattaaacgt ttttctattt ttaagcgtaa tttatt
//
LOCUS       DARBEC010000004        55916 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.004, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000004 DARBEC010000000
VERSION     DARBEC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 55916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.004"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            365..685
                     /locus_tag="SCL80_000262"
     CDS             365..685
                     /locus_tag="SCL80_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEG1106160.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            839..1744
                     /locus_tag="SCL80_000263"
     CDS             839..1744
                     /locus_tag="SCL80_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1106161.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            1741..2514
                     /locus_tag="SCL80_000264"
     CDS             1741..2514
                     /locus_tag="SCL80_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1106162.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(2695..4119)
                     /locus_tag="SCL80_000265"
     CDS             complement(2695..4119)
                     /locus_tag="SCL80_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1106163.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            4330..4776
                     /gene="nrdI"
                     /locus_tag="SCL80_000266"
     CDS             4330..4776
                     /gene="nrdI"
                     /locus_tag="SCL80_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEG1106164.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(4841..6823)
                     /locus_tag="SCL80_000267"
     CDS             complement(4841..6823)
                     /locus_tag="SCL80_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1106165.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(6837..7733)
                     /gene="murQ"
                     /locus_tag="SCL80_000268"
     CDS             complement(6837..7733)
                     /gene="murQ"
                     /locus_tag="SCL80_000268"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEG1106166.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(7736..8797)
                     /locus_tag="SCL80_000269"
     CDS             complement(7736..8797)
                     /locus_tag="SCL80_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1106167.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(9008..9574)
                     /locus_tag="SCL80_000270"
     CDS             complement(9008..9574)
                     /locus_tag="SCL80_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1106168.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(9602..11104)
                     /gene="eat(A)"
                     /locus_tag="SCL80_000271"
     CDS             complement(9602..11104)
                     /gene="eat(A)"
                     /locus_tag="SCL80_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1106169.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(11362..12483)
                     /locus_tag="SCL80_000272"
     CDS             complement(11362..12483)
                     /locus_tag="SCL80_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1106170.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            12609..13550
                     /locus_tag="SCL80_000273"
     CDS             12609..13550
                     /locus_tag="SCL80_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1106171.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(13700..14596)
                     /locus_tag="SCL80_000274"
     CDS             complement(13700..14596)
                     /locus_tag="SCL80_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1106172.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            14865..16205
                     /locus_tag="SCL80_000275"
     CDS             14865..16205
                     /locus_tag="SCL80_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1106173.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            16423..16866
                     /locus_tag="SCL80_000276"
     CDS             16423..16866
                     /locus_tag="SCL80_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1106174.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(17370..18788)
                     /gene="nhaC"
                     /locus_tag="SCL80_000277"
     CDS             complement(17370..18788)
                     /gene="nhaC"
                     /locus_tag="SCL80_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEG1106175.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(19191..20414)
                     /locus_tag="SCL80_000278"
     CDS             complement(19191..20414)
                     /locus_tag="SCL80_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEG1106176.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(20445..21284)
                     /locus_tag="SCL80_000279"
     CDS             complement(20445..21284)
                     /locus_tag="SCL80_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1106177.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(21301..21981)
                     /locus_tag="SCL80_000280"
     CDS             complement(21301..21981)
                     /locus_tag="SCL80_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106178.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(21983..23056)
                     /locus_tag="SCL80_000281"
     CDS             complement(21983..23056)
                     /locus_tag="SCL80_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1106179.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(23112..23350)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(23393..23626)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23703..24374)
                     /locus_tag="SCL80_000282"
     CDS             complement(23703..24374)
                     /locus_tag="SCL80_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1106180.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(24461..27139)
                     /gene="mgtA"
                     /locus_tag="SCL80_000283"
     CDS             complement(24461..27139)
                     /gene="mgtA"
                     /locus_tag="SCL80_000283"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEG1106181.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            27798..28235
                     /gene="ndk"
                     /locus_tag="SCL80_000284"
     CDS             27798..28235
                     /gene="ndk"
                     /locus_tag="SCL80_000284"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEG1106182.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(28257..29123)
                     /locus_tag="SCL80_000285"
     CDS             complement(28257..29123)
                     /locus_tag="SCL80_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1106183.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLAHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(29283..31376)
                     /locus_tag="SCL80_000286"
     CDS             complement(29283..31376)
                     /locus_tag="SCL80_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1106184.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(32125..32748)
                     /locus_tag="SCL80_000287"
     CDS             complement(32125..32748)
                     /locus_tag="SCL80_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106185.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(32907..34304)
                     /locus_tag="SCL80_000288"
     CDS             complement(32907..34304)
                     /locus_tag="SCL80_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1106186.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(34336..35307)
                     /locus_tag="SCL80_000289"
     CDS             complement(34336..35307)
                     /locus_tag="SCL80_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1106187.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            35681..36106
                     /locus_tag="SCL80_000290"
     CDS             35681..36106
                     /locus_tag="SCL80_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106188.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(36594..37295)
                     /locus_tag="SCL80_000291"
     CDS             complement(36594..37295)
                     /locus_tag="SCL80_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1106189.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(37311..37751)
                     /locus_tag="SCL80_000292"
     CDS             complement(37311..37751)
                     /locus_tag="SCL80_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1106190.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(37751..38326)
                     /locus_tag="SCL80_000293"
     CDS             complement(37751..38326)
                     /locus_tag="SCL80_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEG1106191.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(39174..39653)
                     /locus_tag="SCL80_000294"
                     /pseudo
     CDS             complement(39174..39653)
                     /locus_tag="SCL80_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(39812..40513)
                     /locus_tag="SCL80_000295"
     CDS             complement(39812..40513)
                     /locus_tag="SCL80_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1106192.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(40621..42030)
                     /locus_tag="SCL80_000296"
     CDS             complement(40621..42030)
                     /locus_tag="SCL80_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEG1106193.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(42078..43943)
                     /locus_tag="SCL80_000297"
     CDS             complement(42078..43943)
                     /locus_tag="SCL80_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1106194.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGKPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(44064..44915)
                     /locus_tag="SCL80_000298"
     CDS             complement(44064..44915)
                     /locus_tag="SCL80_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1106195.1"
                     /translation="MKIDKILNNNVVISKNGFGEEVVCMGKGLAFQKKIGDEISPEAV
                     QKEFVLRDSFAKNQFQQLMADIPAEEIEWVKQVVELAEDKLKVEFSPNIYLTLTDHLH
                     YAITRAKENIQLPNPLLFETKKFYPKEYQAAKEALLFVKQKTGVELSEDEAGFIAFHF
                     VNSQQGNEDMQVTMTATTMVRDILSIISKFFGIVFDEESLNYQRIITHLQFFAQRYLK
                     GEGSDEQDEFLYALVQGKYPKAFHCVERINEYLRQTQKQEMGIAEQIYLTIHIQRVVS
                     EKKSIQS"
     gene            complement(45225..45938)
                     /locus_tag="SCL80_000299"
     CDS             complement(45225..45938)
                     /locus_tag="SCL80_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEG1106196.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(46006..46332)
                     /locus_tag="SCL80_000300"
     CDS             complement(46006..46332)
                     /locus_tag="SCL80_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1106197.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(46455..46682)
                     /locus_tag="SCL80_000301"
     CDS             complement(46455..46682)
                     /locus_tag="SCL80_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1106198.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(47004..48257)
                     /locus_tag="SCL80_000302"
     CDS             complement(47004..48257)
                     /locus_tag="SCL80_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1106199.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            48318..49058
                     /locus_tag="SCL80_000303"
     CDS             48318..49058
                     /locus_tag="SCL80_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1106200.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(49144..49902)
                     /locus_tag="SCL80_000304"
     CDS             complement(49144..49902)
                     /locus_tag="SCL80_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1106201.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(49899..50927)
                     /locus_tag="SCL80_000305"
                     /pseudo
     CDS             complement(49899..50927)
                     /locus_tag="SCL80_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(50947..54114)
                     /locus_tag="SCL80_000306"
     CDS             complement(50947..54114)
                     /locus_tag="SCL80_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106202.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(54338..55222)
                     /locus_tag="SCL80_000307"
     CDS             complement(54338..55222)
                     /locus_tag="SCL80_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1106203.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(55317..55754)
                     /locus_tag="SCL80_000308"
     CDS             complement(55317..55754)
                     /locus_tag="SCL80_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106204.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
BASE COUNT        16087 a        11751 c         9621 g        18457 t
ORIGIN      
        1 tttttttatc acaaaaatta tcttacttat tgtaaggtaa agcttttttt aatttattgc
       61 ccgataatat atagaaataa ggtctagtta ctgttttttt taaatgatta ataaataaca
      121 acttctttct atgaaattgc aaagcattca tcttcttttc cgctaacgat atatccgtca
      181 gtgcttggta cactttacta aaaagtatga gattgccgat atctggatag gtgtgctata
      241 ctacagaaaa agatatacaa tctgccaccg ggtaggataa agaggatgat cattccatta
      301 ggtctttgaa gggatgattt tcctcttttt gtgtattttt ttatagttaa ggaggaatgt
      361 aagtatggca tggatggaat taatcatagc aggaattttg gaagtattct ggtcaacgat
      421 gatgaaatgg agtgatggtt tttctaaaat caattattct ttttacacag tgattggcat
      481 gattgctagt ttttactttt tgtcaaaggc aatcaaaagt ttgccgatga gtctctctta
      541 tcctatctgg acagggattg gcgcagttgg ttcggtcata attggtgtgg tcttgtttca
      601 tgataagctg aatctttcca cttggttctt tgtggggatg ctactcatca gtatcattgg
      661 catcaagatt actagcggcc actagacact tggaataagt actgatcaga aaaaataagc
      721 ctttttattt aatattcaat ttttttatga aaaaataaga aataagtcca atgacctagc
      781 gacttctcca ctcatcaggt gtatgcttgt actatctata gaaaaggagt gaatattttt
      841 gaaaagtctt tcggtccaag aattatccaa gaactttggt cacaaacaag ccctcagcga
      901 tgtcagtttt agcttatccc ctggcaaaat cgttggatta gtaggtccta atggcgctgg
      961 aaaaacaacg atcatgaaag ctattttagg actgatccat tactcagatg gaacaatctg
     1021 tattggtgaa aatgagattt ctccctctag tcataaaggg ttggaacaag taggagcatt
     1081 gatcgaatac ccgggtatct atccattctt aacaggctat gatcatctga agttattctc
     1141 tgaaaccaac gatgtatctg ctatcgacac tatcgtgaat cagctcaaga tggaaaagta
     1201 cattcacaaa aaagcgaaat cctactcgtt gggaatgaaa caaaaattgg gaattgctct
     1261 tgccttactg aaccatccgt ctatcgttat tttagatgag ccgatgaacg gtttggatcc
     1321 acaagcaaca agagatgtgc gaaaaatcat taccgacttg gctactcagg gcacctcttt
     1381 cttgatctcc agtcacttac tcagcgagct tgaaaaaatg gtcgatgagc tgatcctgat
     1441 caatcaagga aagatcatca aacaatgtac aatggctgaa ttgaaccaaa cagcgcaaga
     1501 tttcatcgtt atctctacaa caaatgatct agctgctcga acgatcttga cagaagctgg
     1561 atatactttg gaagatcctg ataaaatcaa gcttttaaaa gaaaacgaag aattattagg
     1621 aaccgtgatc cagctattgt ctgcacaaaa tatccatgtg acagacgtcc agcatctgac
     1681 gaatgatttg gaatcttctc tattggaaat tctggattca gtaaaggggg aagaagaaaa
     1741 atgacattgc ttgtaaaaca agaattattt aaattgatca agaaaaagtc aactgctgtt
     1801 ttatctgttc tattagttgt tttgctcata ggaacagcac ttctagccaa aaaatacacg
     1861 acgatcattg atccagttga aatgactgct cagcttttta gtgccacatc ttggattgtc
     1921 tttatcatga ttgcagctgc aagcacgatc atttcgatgg aagcacaata tggtacattg
     1981 aaaaatctcc tttatcgaaa gtattctcga ggagaaatat tagtaagcaa atggatcaca
     2041 ttagttattt attctgttta tttatacctt ttagctatta ttgtcactgt gttgatgaaa
     2101 ctgattcttt tcccttcgat ttccttcacg gagaaggttt ctactggtca aacactgatc
     2161 caaagcttgt tcacttatac tttgggtagt tatatcggtt tatggctgat tttaagttta
     2221 gtgttgatga ttgcttgctt tatcaatagt tccggcgcat ccatctcagc aggaatcgtc
     2281 ttttattttg cttcgtcaat catatctggt atccttattg cactcatcca aaaatgggag
     2341 tggatcaaat ggaatccgat cagtatgttg aatctgcaaa accaaattgg aaacgaagag
     2401 atcatgaagc aattgactca tctttctact aatcaaatgc tattcggtaa tttagcttac
     2461 atcgttttgt tcttagcatt aggatatctc gtatttaagc gaaaaaatgt atgatgactc
     2521 atcaaaaaag aggctgtgaa agagacgttc agccttattg ccgattggct gtctcttaaa
     2581 caccgtttat tctgaaaaag gagctgtgac aacatttgtg tcacagctcc tttcctagta
     2641 tgtttttgaa taaacgacag gctcacaacc tctctttata cttcttttat acttctatac
     2701 gtgaagcatc ccagtggcaa ataaaaccat tgcaatcact ccgaccatcg tcacacctat
     2761 aagtaaaatc ttatctaagt ggatatgttg tacaggctcg ttttgctcct tacgtgcttt
     2821 gaggtaaaaa taaatgcctg gcaagaaaag aacaagaaca agcattactt cattaaaggc
     2881 tacgactagc atagcccata cttgaaaggc acttgctaaa agccctacta aaaattggac
     2941 ccattctttc cgctgataag aaagtttcat ttggtacaat cctaagaaaa gccacgtgaa
     3001 gaaaatagct gctgtaccaa gtgtgtaagc aaagttataa gcactgtcag tgaataataa
     3061 tgaaagtaaa aagacattcg tacataacgt cgtcaatacg atagcataag ttggtgcccc
     3121 atttttgttg agttttcccc atgctttagg caaaagtccg tcttcagcca tgtttcccat
     3181 tgtttcaatc ggtaagatcg tccatgagag ccaaacacca actaaagaaa tgatcaaacc
     3241 gccgttgatc acgactgctc cccatttacc aaccatcatt tcaaacacat agcccatagc
     3301 aggctgcgcc ttgatcgttt gcagctgatt catcgtcatt gtcccatatg gaagcatgga
     3361 aaccaagacg taaataataa tcaagccgaa tatccctaga atcgttgctt tcccaacatc
     3421 tgattgtttt tttgctcgtg aacctacgac agaggctcct tcaataccaa caaataccca
     3481 aagcatcacc atgatgcaac tcaatacttg actggaaaac gggatcggct gtccattcgc
     3541 attcaatata atgtcgctag aaaagttatc aatgaattgt tggatcgaaa acgtgctgat
     3601 agctactaca ataaataaaa ataatggcac taatttacag aatgtgacga tagcattgac
     3661 gaaactagca ttttcgactc cccgattcac tagccatgca tatccccata aaaagactgt
     3721 actcatcacg atcgatgcca gattttgccc accttcaaat actgggaaga aatatcctaa
     3781 tgcgctcatc aacaatgttg caaaagctac gttgcctaac cagcatgtga gccagtaacc
     3841 ccaaccgctg ataaaaccag cgaaacggcc aaaaccttct tctgcgtaac taaagatccc
     3901 gccttttaaa tctggacgtt ttttattgat attattgatc gacaaggaga gcatcaacat
     3961 tgctgttcct acgagtaccc aactgaaaat agctgggccg ccagccgatc cggagatgtc
     4021 cgtcataata ccaaatacgc cacctccaat cgctgaacca attactaagg cagcaagtgg
     4081 aattaaacca attccttttt tctgttgctc tgtatccatg taagcatcct ccaaaataaa
     4141 accgctttta tcaaaaagta taacaaacct tttttatttt tacttaatta ataaaaactt
     4201 taatagaaat ataattctac cggctttttt ctcattatac aagcaaagcg cctagtccct
     4261 ttccaaatta caccaccaaa aaacttaaaa aagatgctat actaaaagga aagaattgga
     4321 ggatgatcca tgaacatttt atatatatcg atttcaggaa atacgcgtgc ctttgccaaa
     4381 catctagcag aatatgctga aaaaatgcat gcagaagatc ctgtcaatcc tgaagttact
     4441 ttaaaagaaa tccatgaaaa tagtgatttt acgaaagaaa cagaaccatt ttttactttc
     4501 gttcctactt atttagacgg cggaaatggt ttagataacg gtgatacaga aatcttgacc
     4561 gaaacgatgc gtgaatatct ggaacatgaa gacaatcata aactttgtct tggtgtagtc
     4621 ggtagcggaa ataaaaactt caataatcag tattgtctaa cagccaaaca atatgcacaa
     4681 acatttggct ttcctttctt ggcagattat gaattacggg gaacaccatc tgatgtgaca
     4741 cgaatttatc agattttagc agaaaacaat aaataataaa aaagaagaag ctgaaaaata
     4801 gatttcagtt tcttctttcg tttctattgt ttctatcata ttaagctttg acatccatca
     4861 caggtgcttt tgcttttgct ttacctgttt tttccaagac aaagtttttt acacgatcca
     4921 tattggttac caagacgatc atggatgttt ctttgcctgc tgctttgatc gcttctaagt
     4981 cagcttctgc tacaagcgta tcaggtgtca cttgctgccc ttctgttact ttcacatcaa
     5041 atggcgtacc gcctaagtca actgtgttga ttcccatatg aagcaagatt tctaaaccat
     5101 ttggcgttgt gatcccaata gcgtgtttcg ttgggaagac agagctgact accccagtaa
     5161 ctggagaata gatatgtccg ttttctggtt ctacaaagta gccttcaccc atcatctttt
     5221 gtgcaaaaac tggatcactt gctttatcta aagaaacaac tgttccgtca gcaactgatg
     5281 agaggatagt cacactttca gctgaagttt gaggtactac tttttcaggc acttcattta
     5341 ctgtggctgt ttcagtcgct aattcttctt tttctaaacg gtctttagga ataccgaaga
     5401 agaatgtagc aacgaagcca ccagcataag ctgccaataa tccaaggatg tagcctaacc
     5461 atttaccgtc agagatcaat gggatcaaag cggctcctga aggtccaata gcgatagccc
     5521 cgacatttcc gatcattccg atcactgccc cgccgatacc tccaccgata caagcagtaa
     5581 taaatgggcg tcctagaggt aatgttactc cgtaaatcaa tggttcgccg attcctagga
     5641 tcccgactgg taaagctcct ttgatcattt ctgttaattc tttatctttt ttgcagcgaa
     5701 tccataatgc caatgctgca cccacttgtc ctgcaccagc catcgcaaga attggaagca
     5761 atagcgtacg accagtttca tcaatcattt gcacatggat tggagtcaaa atttgatgca
     5821 gtccgaacat aaccatcggc aagaataatg tgcctagaac gaagccggca aatgctccac
     5881 caacgccaag tacccagttg atcccaccga ccatgccatc agaaatgaaa cctgctaaag
     5941 gcatgatgaa gaaaatttca atcagaccga tggcaatcaa tgaaagagtt ggtgtaacga
     6001 tgatatctac cgcatcaggt actactttat gcagtttttt ctcaacgatt gacaatagcc
     6061 aaacagcgaa gatgacacct aggatcccac cttgtccagc tgcaagagct tcaccagtaa
     6121 ataagttttt cagtggtgct tccggattca tccctgtcag catcacaacg gccccgataa
     6181 ctccaccaag agttggtgtt gcaccaaaaa cttgtgcagc attgatacct gtatagataa
     6241 ctaggtagct aaacatcccg tttttcaaga tgttcatcac atcgatatat tgttgccatg
     6301 ttgctgcatc aagtgttccg gctgtgacta agtttgctaa aactgcggca atccctgcca
     6361 cgatcccagt accgacaaat gcaggaatca ttggcacaaa gatattagaa atgtctttta
     6421 ataccgattt gattttagat ggtttttgtt tcgatttttg tgccgctttc atttcttgtg
     6481 cccgttcgtt tacacggtct tttccagatg ctgatgaatt tgctgtagct gtacttacag
     6541 aagaaagttt ttctcctaat ttcacgcctg ccaaatcaac catttgctgt gctactttgt
     6601 tcactttccc aggtccgatg atgacttgaa gctgttcatc ttctactaca ccaagcactc
     6661 ctggaatcgc ttttaattca gagacattta caagatcatc attgatgatc ttcatacgca
     6721 cacgagtcat gcagtggaca atgctttcta cattgcccat gccgcctgcc cctttataaa
     6781 tttgttttgc cagttcttcc aaagtcagtt cgttttttgc catggtcttt tccccctcat
     6841 aaggtccctt ttacaaatcc gtttgcttca gctaattttg cttgagcttc ctctttatca
     6901 ctatttgtca aaatcatgac gatagccagc ttaacatttt gatctgcttc ttcaaagtac
     6961 ttttcagctg tttcgtaatc agcttctgtc gcttgcatga tgattcgttt tgagcgttcg
     7021 attagttttt catttgttgg tttgacatcg accatcaagt tattgaatac tttacctata
     7081 ccaatcatac tgatagtaga aatcatattc aagataagtt tttgtgctgt tcctgacttc
     7141 aatcgagtag agcctgtcaa aaattctgga cctgcatcga cttcgattgg gaaatccgcg
     7201 tgtttactga tttcggcttg tttattgcaa gaaatcgttc ctgtgacagc accgatcgat
     7261 tttgcgtaat ctaatccacc gatcacatat ggtgtacgac cgctagctgc gataccaata
     7321 actatatcat tttttgtcaa attcagcttg attagatctt cttgccctag ctcataagaa
     7381 tcttcggccc cttcgacagc tacagtcatt gcttcttctc caccagcaat tagacctatg
     7441 accatttccg gttctacacc gaatgtcggt acgcattcag cagcatctaa cacacctagt
     7501 cttccacttg ttccggctcc catataaatc agacgtccac cttttttaaa ggactcaatc
     7561 gtgttagcaa tcactggttc gatacttgtc agttcttttt caaccgcagc ggccactttc
     7621 tgatcttctt tgttcattag ttgaacggct tgagaaatac tcatctcatc taatccaaaa
     7681 gtttcttggt ttctgcgttc cgttgttagg ttctctaaat tgattttgct catttttatc
     7741 ttccttcttt tcttataaat tgatagttca taccagcttt gatttggttg atcaatggta
     7801 gctcttcttt gataacttta gctactacat tcactttttc atccgctggc aaatcatatt
     7861 tggttaattg gatctcaccc atatagcgca aatacttttc gttatctaaa gtaatagacc
     7921 ctttcatacg cgtagctgta tttcttgctg ggataggcgg aatctctcga aaacgtgcat
     7981 ctacgcttcg tatgacatct cttgcatcgt cttgccgatt cgtatgcttc cctaatatat
     8041 attcgtaaaa ttcttcatct acaggttccg tatggagcaa gactgtctgt tctttttgga
     8101 aagaagcaaa ttgctcctga acgttttctg acagacctgc atctccgatg taaacaatat
     8161 ccgtgttact tgctaataga tctagagcgc ctgcaagagg atggatccct cgatgttctt
     8221 ccaaagttgg caatccttga tatagtgggc ctctcaactc tgtatctcct ggtacaaaac
     8281 cttggacaag gagaccttga attttgagcc attgattttt tttctgatac cattctttgt
     8341 ccagccctgt ttcgggacgt ggataataat tatgccaagc ttcaatctgt gagaaatcag
     8401 cttctgcttc tttcaattca tcgatatctt taggcgtgat cgtgctagca ttcaaactga
     8461 ttttcaattg atgtgaccag tcggcaatct gctgattgga aatatgataa tccattctta
     8521 aacctgttac accaattgct ttcaaaggcg ttagatcttc taatgaaaaa cccgctcgtt
     8581 ccaatgcctc tcctgaaata tcgaccatca attctagttt attctttttc gcttcgcttc
     8641 ctagttcgat caatcgcttt tgatatgccg aaacatcatc ttctggtata tgcatggaag
     8701 tgaaaatacc gacaaagcca ttggatgcca tttggcggat atactgtttc ttctcttctg
     8761 acaaagaatc gttcataaat attgaaaagc caaacatatt atccctcttt ttctgtatcc
     8821 gtcttcattt ctatgactag agtatatatc taaagaaaca aaatttcaat acttttatta
     8881 aaataattaa atttcatttc taaaggttga gtttatatta tcaaaagaat tgtcataacc
     8941 taaaaaaggc actgctaagc aaaagcagtg ccttgaaata ttttatttaa ataacacaaa
     9001 aacataatta ctcataagaa accaattctg tattatctac catgaatgta aagaacccct
     9061 cattaggcat cggatgaaaa taagcattga tcactcttat caaggcatta tggcaaacta
     9121 acagatagac attttcttcg tcttcgatgc attctttcaa taaaggaacg atccgtgcat
     9181 acacatctaa gactgactcc ccatttggga atcgtacagc gaacgctaaa cgtgcttttt
     9241 gaaagttctc atctttgctt ggcagtccat cataatcccc aaaatccatt tctaccaatc
     9301 gttcatcaac ggtcagcggc aagctgagac gatcagcaac tgcttgtgct gtatctcttg
     9361 ctcgttgaag aggggaatgg atgattttcg tgattggaac aggcaaatct actacctttt
     9421 ctgctagttt ctgtgcctgc atctgtcctt tttcagtcag cggcacatct gcccgtccgc
     9481 aaactaatcc ttgtgcgttc caagttgttt ctccgtgtct cgttacataa agcatgtgat
     9541 tctctccttc gttggcaaag ttccccttaa ccataccttg ttgttcttga gattacaagg
     9601 gttagattct ctcaagagaa ataatctcag tagatacttt atccaggaag gtttgatcat
     9661 gttccactag taacatggca ggtttcacgt tcaagatcag ttgttctaat tgttcttgat
     9721 tgaagacatc caaataattc aatggttcat cccatgtata tagttcagct ggctgtgata
     9781 aagatttagc caattccact tttttccgtt ggcccatact catctgctcg atcttgttat
     9841 gaaaaacatc tctttccatc ccaagctttc ggaggttgtt caaaaatgct tggtagtcta
     9901 cttggttttt ctctgcaaat tccgccaacg ttcctcgatt gtcttcataa ttttggcttg
     9961 cataactaat gctcagatgt tttggctggt atttttctcc tataaccttg ccgttgaatg
    10021 cccccagaag ataatggata atggatgact ttcctgcacc gttaggacct gaaatcgcca
    10081 cccgctgatg aggctcgatt gtaaaatgaa ttggctcgaa taacagattt tcatacccta
    10141 attgaagatc ttctacgctt aaaagtcgct tatggtgaga cgcttggcta ttcatcgtca
    10201 aagaatcgat atattcgata tcttttaata gtttttcttt ctcgctgatc tgggtctcca
    10261 tccgattcac gattgctttg gatcgtttca tcgtccgtgc agcatcagca cccactgctc
    10321 ctttattcac tcgtctagat tcagtatcga tgaatccgac ttgtttcttt gttttatctc
    10381 cttctcggga acgagaccat tcggcttttt cagctgctgt tttctttagc ctactgactt
    10441 cttttttcaa tttttcattt tgagccattt caaattcatc acgaagtttt ttctgttctt
    10501 catagataga gaaattccct tgataaagtt ccagttgact tttttcgatt gctaaaacat
    10561 ggtccactac ttcatcgata aatccccggt catggctgac cacgatgaag ccttgttttt
    10621 tctttttcaa ataagccgct acttgttttc taccagagat atccaaatga ttcgttggtt
    10681 catcgattaa cgggaaatga gtgtcatcca caaataaaag tgccagtagg acttttgtct
    10741 tctctccccc cgatagtgtg ctgaattctc tccataagat ttcaggatct gtttgcatca
    10801 attggagctc tctttcgatt tcccatatct caaaatccgt aatatcattt aacacgtaat
    10861 aggttaaacg ttctttatct tttgtctgtt gcgggaaata ggcaaattct tgctgatgga
    10921 ttacttgccc ttgataaggt agtttgtttt gtagaatatt cagtaaagtt gtctttcctc
    10981 gaccgtttcg tccgataagt cctagtttcc attgtgtgtc aaaatttaga tttgcttgtt
    11041 caaataataa tgtgccttgg ctgtcgtagc cgaatgtcag attttttatt tcgattttag
    11101 acattttcca tcacctcgta aaaaaagacc agtcctattt tcagaactgg ctaaagagga
    11161 tgcaagggtt ggatacacac aaaaagaccc atacatcgaa aaaaccaagc caatcccgaa
    11221 aatctgcaca ctgcttcctt gtctccacaa ggtacagtct gaatgcagct ttcatcaatc
    11281 gacttaattt ttcaatgcac ggatccctca ctttgtccta tatttgaaat tacgatagca
    11341 taaagaaaat gattttgtca actacttcgg gaggatggtg aaaaatctgc taagtgtctg
    11401 tcctaagatt tttgtttttt ctaatgtttg cctgtgttcc attgcttctt ctaaacctat
    11461 tggacccaaa tgaatactta agacaattgg gagaaaatca gaaagtcctt ctagctcttg
    11521 gtctctactt ccgcaaatag cgattattgg ttttcgatat ttttttgcaa gcactgctac
    11581 accgtatgga acttttccgt ggatcgtttg ctgatcgatc cgcccttctc ctgtaatgat
    11641 gacatctgct tgttcgatct ttttttccaa tccagtgatt tggctgacta aatcgaaacc
    11701 agaaaccatc ttcgctccca acaaaaaaag ggctccacca ataccgcctg ctgctccact
    11761 tcctggaata tcatctaaat ccacctccca gaaatctttt atttgctgag caatctttcc
    11821 tgcttgttta tctaagaaag ctagcgtcgt atctattcct cctttttgtc ttccaaattg
    11881 ccaagcagct ccttgttgac ctgtatatgg attggtaaca tctgcagcga taatcaattt
    11941 tactttagaa acaagaacgt ttcttcgatc aaaatgaagt tttttagtag ataataaagg
    12001 atttccttct gtgtaccctt ctaaagaagc acccaaggat tggagtaatc ctagaccacc
    12061 atcgctacac ccgcttcctc ctaaagtgac aataatttgt tctacttctt gaagcagtgc
    12121 atctttaata agttctccta aggcataact ggaagcaagt cggatagttt gatccgttgg
    12181 tttatcaagt agatctaacc ccaatgcttg tgcagattcg atgactgcgg tgttttttcc
    12241 ttgccaagtt gtcaataaat agctgatttt tcgctgacgg aataagagat ccatcccatc
    12301 aatggtacgc catgttcccc tgagcgcgta atagattgct tctgcagtat tttctccgcc
    12361 atctgcaatc ggcacattga tttttcttcc tttacgatct tcccagccat ttagaactgc
    12421 ctgatttagt tcaaatgaag aagcacttcc tttaaatgaa tcaattgccg ccaaaatatc
    12481 catctgtatc cctcactttc ttcttaagta agacactttt tttcattttt ttcaaacaaa
    12541 acaatgctat tttcttccat ttttctatat actaaaaggg aactcattga ataaaggaga
    12601 gaaaaaacat gaaaatcgca atagctgggg ccggagcaat gggcagccga atcggattga
    12661 tgctacatca aagcggaaat gaggtcctct tgatcgatcg ttggcctgct cacattgaag
    12721 ccattcgtac aaatgggttg attgctgact ttaacggtaa agaagtggtc gcaaaactgc
    12781 caatctattc accagaagaa atcatcgaaa gcaacgaaca cgtggacttg atcgtagcat
    12841 taacaaaagc caatcaatta gatgatatgt tttgttcgat ccaatccatt attactgaca
    12901 atacttatgt tttgtgttta ctaaatggat taggtcatga agatgtactg gaaaaatatg
    12961 tgccaaagaa aaatatctta tttgggatca cgatgtggac agctggtcta gctggtcccg
    13021 gaaaagtcac attgttaggt gatggagaaa tcgaactaga gaacttggaa ccagaaggcg
    13081 aagcatttac aaaaaaagtt gtagaagtct ttcaagaagc aaatttaaat ccgatctata
    13141 gccataacgt tcgttattcc atttggcgaa aagcttgtgt aaatggaaca ttgaatggtt
    13201 tatgcacgat tttggattgt aatattgctg aactaggcgc gcaaaaagcg gcagagagca
    13261 tggttcgaac aatcgtatct gaatttgctt ctatcgcagc aaaagaagga atcatactag
    13321 atcaagaaga agtgtaccaa catattgctt ctacttatga tcctgacaac atcgggttgc
    13381 attacccatc tatgtaccaa gacctgatca aaaaccatcg tttgactgaa atcgactaca
    13441 tcaatggtgc catttggcga aaaggacaaa aatatgatat tgctacacca tattgtgcct
    13501 ttttgaccca attagtccat gcaaaagaag gaatccttgg cgcagaataa aaggctctct
    13561 gatgagaatc agctgaatca gcagctggtt ctcttttttt aagcccaata aaagatcaaa
    13621 aagaaaacac aactctcatg atgccataag aattgtgctt gtttgtttca gtacataatc
    13681 tcttcaaaat gtaggttttt tatttgtttt caacagagga gaggtcatat tcgtattgct
    13741 cataaatagt ggaatcgtta gtgataaatc gttttgccca attttctgca tcataaaatt
    13801 ccaatagctc tgataggatt ggcttaaatg gtgtatgcag aaattccggt aattcatcta
    13861 aatcacgttc tcgaagcaaa aaaatatttt caggacgata aaaatgataa tgagcaatcg
    13921 cttgattatc cgtcactagt ttcttttgaa aatacatgga ttcaaatgtc cgcaaagtca
    13981 ctccttgctg accctcccgc aacagttcca gaatcccgcg cgattcccct gtttttttca
    14041 ggtactcttc ataagtaaga tatccaggtt gttcattccc tcgatgtgga agaatgaaat
    14101 agttggtcgt aacatctctt tttataaatt cctgcttata gctttctgct cgttcttttc
    14161 tcccattatt cgttgcacca aaaaatagat ccgtctttgg ttcgctagaa gaaaaattca
    14221 tatgtttcga ataaaatgaa ctattatgtt tcagcccaag ttcttttgct tccaaaaaat
    14281 caaaatgata aatctcatcg attgccggtt catcttgtgc ctgcttcagc atcgcctcgt
    14341 gttctgcttt gaagtgattc caaaaaaata acacaatttt tgcattggta aatgggcgca
    14401 cgcgtcgagc catctccata tcaaatccat aatctaaaaa aacgatcagc tgataatttg
    14461 ccaactgttg tctagctgat ttaatgaatt ccgactgtag taaatctttt ccggaccaaa
    14521 catctaaaga gcggatggaa ggcaaaaaat aaagtgattt attttttgca actagcagta
    14581 cgtcattttt gttcatcttt tccgcctcac tttctctgtc actttttcct atgatagcct
    14641 gtttaaatag taaaaagcca gtaaaacgtt tcctatattt gtatgaaaat ttaacatttg
    14701 tatgaaaata tgatgaaata aatcattctt ctattaaaat tcgcctaaaa gttttctgaa
    14761 tagttgtaat tgtctgaaaa tttgttatca ttagtcattg ttaaatattg ctatttattc
    14821 ttaatataaa ttttgattgt gaatttaaaa gaaagaggtc atttatggaa gataaaaaat
    14881 tgaaaaaaca acttagctcc agacatatca ccatgttagc tttaggtgga gcaatcggtg
    14941 caggtctatt taaaggaagt ggcgaagcga ttgggatcgc cggcccttct gtattgattg
    15001 cttttttgat tggtgggctg attttatata tcgtgatgaa aggtctggga aaaatcgttt
    15061 taagcggtgg cgacacgcat cacggattat ctggtttgat acgcccttat ctaggtgctc
    15121 attctgctga ttttactgat tgggtctact ggtctatgtg gattatcaat atcatcgctg
    15181 aagctgttgc agctgcttcc tttttgcagt tgtggttccc gcacattcca gcctgggtat
    15241 ttgtttttat gttggctgtt ttaactacga tcatcaatct ttattcggta cggttattcg
    15301 cagaaacaga atattggctg gcttttgcta aaatcagtgt cattatctta ctgatcattt
    15361 ttgctactta tcttgtcggt cagcaaatgt tgggcacagg cgttttccct acccttcagc
    15421 aattgactga ccatggtggg tttactcctc atggtatgaa gggaatcatc agttctttgc
    15481 ttgtagttat ctattcttac ggcggttctg aactgattgc tatcacggta agcgaagcag
    15541 atgatcctaa acgtgcgatt cctaaagcaa tcaaaggtgt catgggaaga atcatttcct
    15601 tttacatcat tcctttgttt ttgcttttga ttctttttcc ttggaatact ttagcaggca
    15661 cgaatgtcag tccttttgtg atggtatttg aaaaaatgaa tatcccattt gcagcggata
    15721 tcgtcaattt tgtgattgtc cttgctttgt tctcatctat caattcgggt gtgtatgcat
    15781 cttcaagaat tttgttcttc cgtttgaagg atcgaaaagg ttcctctaga aaactagctg
    15841 ttttaaacaa gcatcaagta ccgcaacgtg ctgttttatt ttgtaccagt gttctttatc
    15901 ttggtgtagc tctttcttat tttgtcggtg acaaattatt tggctatctg gcaggttccc
    15961 tttcgtatac agtattattg atctggatca ttatcagtgc ggcatccttt gtactggcac
    16021 taagaaaagg aacattatgg gataaaggaa tcagcttttt tgcattagct gtcttaggat
    16081 tgattttcct tggtatcctg ttcacgaact ccattggggt aactgtattg actggcctgc
    16141 tttacctgtt catttatttc agttatcaaa aaaagaatga tgcgttcgta ttgacgaatg
    16201 aatgaaatcg atagaaaatt ttattttatt tatgtggaat agatggagct attgaagaaa
    16261 ggagttgtga caaaagtctt gttgcaactc ctttttccga ataaacagtc tcatgacctc
    16321 ttggtaaacg gtgttcaaaa accctctgtg caaaataatt gattgttctc catgctcatg
    16381 acatgataga agaaaggaat gaaaaaaagg agtttgtttt aaatgaaaaa gtattggaag
    16441 tttttgttga tgatcggtgt ttcaacggta gttatgtttg gtatgatgta cttgaatgtc
    16501 tatgcgctcg atcatatctt cttcagtcgt acacgagtat tcatggcgtt gatgatgggt
    16561 gcagtcatgg cagtcatcat gctgttgttc atgtggaaaa tgtatgaaaa caaaaagata
    16621 aatgttatga tcttagccgc tagtcttctt gtttttgccg gttccttgtt tatggtacga
    16681 agccaaacta ctgtcaacga cacagcttgg atgaaggcca tgattcctca tcattctatc
    16741 gctatcctgg caagtaaacg agctgattta aaagatcctg aagtaaaaaa attagcggaa
    16801 gaaatcattc aggcacaaga agaagagatc gacaaaatga aaaaaatgat caaagaaatg
    16861 gaatgaccat tatccgtcca aataaaaaaa gaggatgttc cagaagttat cagcttcaag
    16921 aaataagaag aagctacttc tggaacgccg tgaataaggg gttgtgaaaa agctgccctg
    16981 catcaagtaa taagaacagc caaacaaaaa tagcttctcc tatttttcgt ttggttaggc
    17041 ttattaccgc agatgcaagc ttttgaacac cgtttattaa gaggttgtaa aatcagcgtt
    17101 ctgacctgag tattagagag acaaatggaa aaatagttcc tcatattttg ccattttttg
    17161 agctaatacc gagggtcagc tgattgaaca ccgtttatcg agaggttgtg aaaaagtcgc
    17221 ttagtttcgc gtattaagaa aaattttgtg aaatggcttt ccacattttt gtataaattt
    17281 ggcttaatac cgagaaactg gcttttgaat accgtttatt cgtaaaaggg attgtgacaa
    17341 aagtcttgtc acaatccctt tttatgtttt tattgttcta attgcgctgt cttgcttact
    17401 gcttcttgcg gtttcacaaa tttttttgag attccccaac ctgtgaaggc actgaccatc
    17461 gaaaacacag gagaaagcaa actgaaaaat acgaatggca agtaagtgag tgtaggaata
    17521 ccaaatgtac ttgctacgaa acttccggca attccccaag gcactaagta gttgattact
    17581 gttccaccat cttctagcgt tctacctaat acagttggat caaatcctgc tttttcataa
    17641 gtttctttaa atgcattacc aggcagaata acagatagga attgttcacc aataaaaata
    17701 tttactccga tacttgtcat caatgttgta aaaatcaaat ttggtgtgtt cgtcatcttt
    17761 cttgatactc gttccatcac ggttgagatc aagccaaatt cgatcaacaa tccacctaat
    17821 gacaaagtta gaataatcaa tgacacagta ggcatcatac tttcgattcc tccgcggcta
    17881 agcagtgaat cgatttgttg gtttccagta tttgaaacaa accctgattc gatgacccca
    17941 gccaacttcg ttaaggaagt ttgtgggttt tgaataaaaa tcattcctac tgctacagcg
    18001 atattcaaca aaatcgttaa aattgccggc acctttttcc atgcacacag taacattaaa
    18061 aataaaggaa tcagtgacca gattgaaata gtaaagtttg cttctagaac atcagtcact
    18121 tcacgaatag atgaaagact tgcggcttta cttgtgtgtc ctaaaatcat gaataaaaag
    18181 aaagaaacaa caaatgccgg gacagttgac cacatcatat gcttgatgtg tttgaacaaa
    18241 tcggcatcaa caatagctgc tgctagattt gttgattcgg ataatggaga catcttatct
    18301 ccaaaaacgg cacctgaaac aatagctcca acaaccagcg ctggatttaa gcctagtgtt
    18361 gtcccaatac cgaaaaatgc aatcccaatc gttgacataa cagtaaaggc actccctaca
    18421 gcgctaccta cgattgcaca cacaacaaat acagaaggga caaaccactg gacactgatc
    18481 aatttgaacc caatgaccat tagagttgga atgatccctg cttgtatcca tacagcaatc
    18541 aatgaaccga ctaaaataaa aataaagatt gggatgattc ctggtttgat gccattgatg
    18601 attccttcgt gcagccaatc tgtcgggtat ctttttatca ctgcatacaa catgaccatt
    18661 ccgatcgctg ctaagatcgt gatattaggt gagaccttta atccgacaac gccaatcgca
    18721 atgcctaaaa caataaccat taatacaagt gttgcttcta aaaaacttgg tgttcctttt
    18781 tttcccatga taatctctcc ttttttagtt gttcttatgt tctaaaaaag ttaagatatc
    18841 ggattactcc gccgcaattc atccgcgatt cctcctttat aaaaataaaa agacctgttt
    18901 taaactaagg acgaatgact ccgcggtacc accttaatta aaaacagatc tgtttttcac
    18961 tttcaaactt ttattcaaat gtatggactt tccatgtaat tcgtcaatta ggcttgatcg
    19021 gttcacagcg accaccgact ttctgaacaa aacacctaac cgctactgcg tgaaaagttg
    19081 acttgtacga agtttacctt ccataagatc atttgtcaat gaaaaagaaa atattttttc
    19141 atttttctga aaattatttt tataactttc acacacaagt ttatttttta ctagaaagta
    19201 gcgccctctt gtttttttct tgctactatt tcttgatgtt cttgcttgat tctttctaat
    19261 agctttggct ctttcaacag atcaagagct gtattggcga gtagttctgc tgcaatcgca
    19321 atagaagcaa ggcctttgtc acttttagca gcagctttga attcttctgt gtgtccggca
    19381 atatatgtct cagagattgc taccgtaggc tggattgttg gtatgacttg gctgacattt
    19441 cctacatcag aagaaccgag acttgttctt tgttccgttt ctatttcttc atctggaaca
    19501 cctgcttctt ttgcatgctg gaagaataag tcatcaaaag aaggagtaac gatgacatca
    19561 tcaactgcat tttggaacaa tccaaactca aaagtcgtgc ccgtacttat cgcagcacct
    19621 cgtacgatat tttccacttt ttcatatact ttatctaatg tttgccgatt tcctgcacgt
    19681 aaatagaaac gtccagcagc gtaatctgga atgacgttgg cagctactcc tccgtctgta
    19741 atgatcccat ggatattgac atcttttggc aattgcaagc gtagagcgtt gattccatta
    19801 aacacttgga tcaatgcttc caaagcattt actccttttt caggtgctgc tgctgcatgg
    19861 gaagcgacac cgtaaaattt gatgtcgact ggatcattcg ctaaagattc tgtcgttttc
    19921 ccataacgaa aagctggatg gacgcataac gctacatcga catcctcaaa aaaaccttct
    19981 cgaacaaagc tgcctttagc tgatccgttc tcgccccctt cttcccctgg ggtgccatat
    20041 acacggattt ctccaccagt ttcatcaatg aattgcttca ctatgctggc ggctaatacg
    20101 gagtaagtac caaacaaatt atgcccgcat gcatgaccaa tacctggtaa agcatcgaat
    20161 tctgccaaaa aggcaattgt gggaccgggt ttattggaaa tataacgtgc atcaaaggct
    20221 gttcggtgtc cagctacttc tttttctacc caaaaccttt ctttttctaa ttgtttaatc
    20281 aacgctgctg aggaataaaa ctcatagtta cttacttcag gatgttcgtg gatatctaga
    20341 gcaagtgctt gatattctgg taaacgatat tggattagat ccttcaattg gtttgttgag
    20401 attgttgttg tcataaaaaa ttcctcctat gctagtcatt tttcttattt tctcccgaat
    20461 gtttcccatg caggcacgct tgatcctttt gatgttttct cgattacttt gactgtgtct
    20521 tctgtttgat aagcgtctac tattttttga tacgttttat tgttttcatc ttcttttctt
    20581 gcaacgatga tattcacata aggacgtgca tggtcatcca ctggttctaa gaagatggca
    20641 tcagaagcag gattaaatcc agcatctaca gctacaccgc tattgatcac tgaaatgtct
    20701 acatctgaca aagcacgtgc tgtttgggaa gcatctaatt cagaaatgtc tagatttttt
    20761 ctattctcgg taatatcaga aacagtgggc gcttgtcctt ttgccttatc cactttgatc
    20821 aatccagcag tttgcaataa cagaagagct ctgccgccat ttgtaacatc attagggatt
    20881 gctacttttg ctccgtcttt gatatcagct acatccttca aagtgttaga ataaattccc
    20941 agcggtgcat tgacagtatt tccgatcgat actaaatcag ttccgtgttc tttattgtaa
    21001 ttgtctaaaa agaattgatg ttggaaagaa ttcaaatcaa tcgatccatc tgctaaagcc
    21061 acatttggtt gattgtaatc tgtaaatttc acgtattcca aattaatgtt ctcctttttc
    21121 aaacggtctt tcacgttgtc ccatacatct gtatcttctc caataatccc tagttttacg
    21181 atagtttctt ttttgtcagt tgtatctgct tttccttggc tacaccctgc caaccaaata
    21241 attgctacaa atgtaatgac tacaaaacga ataatttttt tcattttttc tcctccactt
    21301 tcaatgtgta atttttttaa tcagttgatt actgataaat tgacttaaaa aaactaagat
    21361 tagaatcaat aatgtagcca caatcgttac atctgtttgg aatcgattat accctctcga
    21421 tatagcaaga ttccctaatc cgcctgcacc tactgctcca gccatggtag tcagtccgat
    21481 cacgttgatg atagtcaacg ccgagacccg aatgatacca ggcaaacctt ctaacaaata
    21541 cacacgtaaa atgataccga ttggactggt tcccatcgct tctgccgctt cgatcacccc
    21601 aggatcaact tctagcagtg cgttctcgat ttgacgagca aaaaacggaa caactccaaa
    21661 aatcaatggc actaaagctg ctttcgcacc aatcgtcgtt ccaactaaga aacgagtagt
    21721 caatgctaaa agagaaagca agataatgaa agggatggaa cgaatgatat tgatcagctt
    21781 atcaagtatt tcaaatagca gacggttagc tagtaatcca ttagggcgtg tcactactaa
    21841 cgtcacacct aagatgattc caatcatccc gccaatgaat gctgtccaaa aagacatata
    21901 tagtgtttgc ttcgtcgctt cgacaaattc atccggaatc aaataaacat tgggcaaata
    21961 tgttttaaag aaatcagtca ttttatcttc ctccttcgat cactttgata cgactatcta
    22021 aatcgttttt ttgtaaaata tttattttta caccagattt gatcaaataa tccaatgctt
    22081 ttttccgctg tttggtttct ccagctaaag taacgatcaa actccctaac ggcgtttctt
    22141 gtaataactc tacattgcca tataaaatat tcgctgttac ttgatatttg ctgtacagat
    22201 gagcgattag aggttcgttc gtctgatttc caatataact taattccacc agccatttat
    22261 tggcgatctg atcggcaaaa gcagaatggg caatgatcgt ttctaatgct tggtcaagat
    22321 gagtagctgt tcgaataaaa tctttggtca attcttcttc aggattgctg aagatctgga
    22381 tgctgctacc tttttcaacg attcttccat cttccataac cgctaccttg ttgcagattt
    22441 ctttgacaac ttgcatctca tgagtgatca ggacgattgt taaacctaac tggcgattca
    22501 atttttttaa caatgcaagg atctgaaggg tcgttttcgg atcaagtgca ctggttgctt
    22561 catcacataa taatacctta ggttcattcg ccaaagctcg tgcgattgct actcgttgtt
    22621 tttgtccccc cgacagttgt ttaggataag cagatgcttt ttcttccaaa ccgacaagtt
    22681 ccagtagttc attcactttt tgacgacgtt cctgtttcga ttttccagaa tactttaaag
    22741 aaaaatcaac gttgtcaaaa atagttcggc tttccattag attgaaatgc tgaaagatca
    22801 taccgatggc ctttctttct tttcgcaatt ctttgggaga gagctgactc agattcgtct
    22861 gattgacaat gacttctcct gctgttggtt tctgcagcaa attcatcaca cgaactaacg
    22921 tacttttccc tgcacctgag taaccgacga ttccataaac atcgcctttt tcaatcgtta
    22981 aatctacaga atctactgcc tgtatctctt tttctttttg tttgaaacat acagaaacat
    23041 ctcgcaattc aatcattaaa attcctcctt atctaaaaaa agcgtcctca agaaaatgaa
    23101 aatgtcattt tcttgaggac gcttttttgc gtgttaccac ctcatgttca agctgtgttc
    23161 acacacaact cctcttcaag tacgatcata ctctagcaag atatcggtcg cagccgtcat
    23221 cgccttacat ctatgctcgg gatgaattgc tcgatggcca ttttccaata gtcgttcctt
    23281 gcttcttctc agctaccgaa gctctctgtc aaaggttctt ctatcgtact taccatttca
    23341 atgcaaataa tattcaaaaa aaaagtcctt ttactaacat aaactatgtc agtaaaagga
    23401 cgacgtatcg cggtaccacc ttttttcgga agtttccttc cctcaagccc aacatctcaa
    23461 aaagagagat tggcgttcgt tttaacggtc gaacagttcc gaatttgcct acttatcgac
    23521 aaattattct ccaaggccat tttcagcttt tcttacttga ctcttctcac cagccagagc
    23581 ctctctgcaa agtttccaaa cttactttcc ttttcaacga aatcgatatt ttgttgttgg
    23641 gattaatcta catgaggaaa actgaaaagt caagtacgaa attcaaatta ttttttctat
    23701 atttattttt ctgttttttt atttaacatt tcaataacag ttcgaagatc ttttccttcc
    23761 gcaagaagtt tcaatttttc cttttcttcg acggtataat ttcggacaaa actggaatca
    23821 ggtatcaaaa caacgcctaa tcctgctcgt gaagcagctg ttgctcctac taaagaatct
    23881 tccactgcaa aggctgaact agcttctaat cctgttcgtt ccaaagctgt cagataagga
    23941 atcggtgaag gcttgttctc cgacacttcg tctccgaatg taaacgtgtc caagtaatca
    24001 gctaatttaa agtgatccaa gatatctgtt gctcttttcg taacagtggt cgtagcaatg
    24061 cctagtttca tatgacgttc ttttaagtct gccaatattt cttttgcata tggttttaag
    24121 tggatggctc cgcttgtcag ctgttgataa aaatattctt cacgcttcgc tcggacttcc
    24181 tgtacttttt cagggctacc tgctgctttc actaaaatat caaaactttg tttccagttt
    24241 tttccgcccc aagcgtcaac gatttctttt gggatatgga tatcaaattg ctgaaatccc
    24301 caaagccagc catcacgata cgttttttct gtgtcaacta acaaaccatc taaatcaaat
    24361 attgcacctt tcatctatac attaatcctt tccaaaacaa tacacatagt ttagcacgac
    24421 tggtccaaaa agaacagaag caaagaacca gccttctcgt ttataagagt tcttggaatc
    24481 gacggatata gattttcttc acagctgtca caagcgccag ataagcgatg actgtagcaa
    24541 ataaccataa ccagtaattg ccaggaagtg gcatcaagcc taattcagca ccaaaacttg
    24601 taaaaggaag gacagaaccg acaataattc ctaatgtagt gataacaaac atggcaaatg
    24661 ttgcgttgct ttggataaat ggtactttcg gtgtacgcag tgcatggagc actaaggttt
    24721 gtgtccatag tgattcgaca aaccatcctg catggaataa tgcgatgaaa atcactcgct
    24781 gtgaactatc caatgtatgg aaacttccgc ccactacagc aggacaaata acaaaataca
    24841 tcaacaagta agtagtaata tcaaatatcg aacttgttgg accaaagtaa accatgaatt
    24901 ccccaatact agatgcattc catttttttg gctcgtccaa atattcttta tccatccgat
    24961 cccatggcaa ggaaatgcag gaaacatcat agatcagatt caaaaacaaa agttgtaaag
    25021 gcagcattgg taaaaatggc aggaacgtac ttgcaatcag cacggaaaac atattgccaa
    25081 aattagaact tgctgtagcc ttgatatatt tcatgatatt cccaaaagtt tctcgtccag
    25141 aaagaaggcc tctttccaaa atcgtcaaat ctttttctaa aaggatcacg tctgcagatt
    25201 ctttcgcaat atccacagct gtatctacag aaataccgat atcagccact ttcattgcag
    25261 gagcgtcatt gataccatct cctaaaaatc cgacagtatg accattttta cgtaaagctg
    25321 tcgtcaaacg tgctttttgc tgaggattca attttacgaa tacttcatgg tcttcagcta
    25381 cttgtgctaa ttcatgatca ctcatttccg agattttctc accggtaacc aattcattga
    25441 tctctagacc tacttgacgg cacactgaac gtgtaactaa ttcgttgtcc ccagtcagaa
    25501 cttttacagc tacaccatgt tctttcagag ctttcaacgc ttgtttcgtt gtttcttttg
    25561 gtggatcaag gaaagcaaga taaccaatca aaaccatgtc tgactcatct ttgactgaaa
    25621 attctccaac gacgcttgga ttggtctttt gtgcgacacc aatcactcgt aagccatctt
    25681 cattaagtga ctgtactttt gcaagtacgg cttcttttcg ttcttttgtt aatggtgtaa
    25741 cagtaccgcc aagatcaata aatttagaaa tgttcagcat ttcttcaatc gcgccttttg
    25801 tgatcatttg tgttttacca gctggatcag caaccacaac actcatacga cgacgttcaa
    25861 aatcaaaagg aatttcatcc actttttgat aattgatctt atctgtatct aaagtttctg
    25921 ttgcttcatc aatgatagcc acatctaaca aatttttcag ccctgtttga tagtagctat
    25981 ttagatacgc atggcgcaaa acacgatcat cttcatttcc ttcacaatcc atatgatatt
    26041 caagaatgat tttatcttgt gtcaatgtcc ccgttttatc cgtacataac acatcgattg
    26101 caccaaagtt ctggatggca ttcaaatttt tgatcactgt tccttttttg gccatcacac
    26161 ttgctccttt gaccaaattc gtcgtaacga tcattggaag catttcaggt gttagaccaa
    26221 ctgctacaga tagtccaaaa agaaaagctt ctagccaatc cccttttgta agcccattga
    26281 tgacgataac tgctggagcc atcaatgaca taaatttgat cagtagcatg gatgttttat
    26341 ggatgcctaa ttcaaagctt gattgaattg gtttttcaga tagcgtttct gctacatgac
    26401 caaatagtgt ttcatttcca acagaaacaa tgattccttc cgcacttcca ctgatgacat
    26461 tacttcccat aaagacaaga ttttcgtaac ttgtttcgct ttgctgatcg tcttcacata
    26521 ctttcgcatg cttttctact ggatagcttt cccctgtcaa tgctgcttgg gaaatgaata
    26581 aatcctttgc ggacaataaa cgcatatccg ctggtatcat atcccctgct gaaagccgaa
    26641 caagatctcc acagacgatt tcttccatag gaatctctgt gtactttcca ttacgtttta
    26701 cagctgccgt tactttcaca agtgatttca gtttttcagc cgcttgattg gaacgtacgg
    26761 actgaatcaa cgtcattgtc ccgctgacga taaccatagt gaagataatg gctacaccga
    26821 aaagatcttt atctccagga gctgctaata catattccgt gatgaatgaa ataattccta
    26881 atgcgatcaa cactaatgta aatggcgtga tgtatgcttt caatatttct acaatgaatg
    26941 gtgttttttt gccatatgaa atcgcatttt caccgtaatt ttctcttgaa cgttcgattt
    27001 gttcttctgt caatccattt gctgatgcat ggaatagttc aaataattct tgtttcgttt
    27061 ttccagcaaa agctgcatag attttttgtg tatctttctt tttttccatc tcaatctctc
    27121 ctttgtgtcc aaaagacagc tgttctcgct gcttttggtc tttagctatt catcggatcg
    27181 atagagaaaa aactgaaagg ggctgagaac agatagataa tagccctggc ctttcctcta
    27241 gatccttgtt tttcgaatga ctgtcatcca tttgttctca tctcctttac taagtaattt
    27301 taattcatga cttccaatca tttttcgaat agttattttt ccaattagac cacctccttt
    27361 ttatttttca ccgatgggga tttgcggcaa caaaaaaacc tctcgccaaa tactcagcga
    27421 aaggtttgaa aaagcatatt aaaaaaagct tctcagatct catcgttgag ttttgacact
    27481 gtacaacgta aaaagcagat acctgctttt agctacctta tgaaaagcct tatctcgaca
    27541 attcctgttc tacccattgg cgtctctcga cgtttttggg cagtagccta tgtttgtata
    27601 ggagcctcac ctaacaaatc ttattcggtt ttccttcgtt aatataacaa ctaaaaaaga
    27661 ggatgtcaaa tcaattttcc atgtttttca tacttttttt aaaatacaca cgttatattc
    27721 ctgtctcacg cggttttatt tgtctcattt tccacctccc gctataatag aaatatagta
    27781 ggaaacgagg tgctgttatg gaaagaacac ttgttattat caagccagac ggcgtgagac
    27841 gccatttagt cggaagcatc atccaacgtt ttgaagcaaa gggactggcc attgctgaaa
    27901 tgaagtttga aactatgact cctgaattag caaaagaaca ttatcagcac ttgactgagc
    27961 gcagtttttt tgatgaactg atcgattaca tgacttccgg tcctgtcgtt tatcttgtgc
    28021 tggtcggcga ggaagtgatc gatatcgtcc gtaagatggt tggagcaaca aaagctgccg
    28081 atgctgttcc aggtacgatc cgaggagact acgcgctgcc ggggactgaa aacatcattc
    28141 acgcttctga ttcccgtgat gctgcggtaa aagagatcgc ccgctttttc ccgccattcg
    28201 atataaacaa accgtctgaa aaagcggcga agtgatacaa attctaaact gctggtctag
    28261 ttgctgggtt cagcaatggc cagcagtttt ttctctaagg acctccgttg attctttcct
    28321 aacatatatt cgagcactgt gaagaattct tggtagtctt tcgtcttttc ttcccctaaa
    28381 gcagcagaaa tcaatctttg ataataatct gccatcaatg tttcatagat cactgtactt
    28441 ctttctttat gtgctaagct ccgtttcata tcttcattca cactacgtgc ctttttcaac
    28501 cgaccatttt ctatataaaa cgaagtcagg ttcattaaaa aagtaaagag atactgctca
    28561 tgattgttca actgtgctaa tcgtcgcatt ctttttttga ttttcaaata attgttctca
    28621 atgaaccgat cactgaatat ccaaggaaaa tcagctagaa tagatagttc aaattgacca
    28681 tagtctggta tctgcatcaa gtattcttca atgattcggc attcataacg taaaaattca
    28741 aattctgtct cagatacttc tgttttttcg accatgtgaa gattcaaaac caaataatac
    28801 agatcttctg ttttcttaaa atgcctacgg ttattgctga tctctttata aggatcaaag
    28861 ctgtcttctt gtacccgatc tagcattcgt ctttttttct ttaactttat tggttcatcc
    28921 agctggtttc gcatccattc atattctgca agtgtcatgt tcattcgctc taaaaatgat
    28981 ctcaatgtta caaatgacat ttttttcact gaatttgtct caatcccgac tagagaagtc
    29041 ttaaaacaga ttccttccgc taattgggac tgtgtcaact tcttgttgac tcgaagtttc
    29101 ttgatcaatt caccatcata cataatagtt ctccttttta atttaaattt ttccacattc
    29161 aataaataga tacgttaaaa aggaaaaata cacgaaaaaa ataaaaaaat gtttttttca
    29221 taaaaaatac caaccttcaa gcattagaat gttgtctaac tactgaaggt tggtttgatt
    29281 gtttattgat tatttttttg ctgttcaacg tctgttcttg ttactggata taagttggtt
    29341 gttgttttat taccattttt tgaaaagaca cttgtcgaat gttcgccttt ttgcttttcg
    29401 attgcttcat tacgttccgc ttcattttta tagtcatatt ttttcggatc aatcgtctca
    29461 aatccgtttg gcacataaaa ccgcaagaga ttttcctgat tcagcttatc tgaaagactt
    29521 aacgcagtct tgactttttc ctgatctttg gagatttcct catcgattcc tgctgctttc
    29581 gtatcgatga tttctcctgt ctgattattg taagcttttc ctcctaggac ggtgtatttg
    29641 ggtgtcacaa agttcccatt tcgaaaagca actacttgat catgctgaga agataataag
    29701 tctgtaccaa aatgaagata atctttatca tcgattccca atagatgtag taatgtcggc
    29761 aaaacatcga tttctccacc gtattcatga ttgactgtcc ctttagtata accaggcata
    29821 tggatcatca atggcactcg ttgcatctgt gcgttgtcaa attcatccca tgtatctgga
    29881 tctttaccaa gagcgcttgc taaatcttta ttatctgtat tcgagatccc aaaatgatcc
    29941 ccgtaaatca caaagattga attttggtaa ataccagatt ttttcaaata cgtaaaaaat
    30001 tgttccaatg attgatccag ataatgtgcc gttcgaacat aattatctac tgtgctattg
    30061 ccagtattca aagatggaaa gtcgaaattc ttgtcatccg tataataagg cgtgtggttg
    30121 gttacggaaa gaaatttcgt gtaaaacggc tgttgtagat gctcaagata ttgggcagat
    30181 tctctgaata aatctttatc taatatccca taacctaacg tttcgtcgga ttcatcaaaa
    30241 taagatcgat caaagaaatt gtcataacct aagtttttat atacatgatc tctattccaa
    30301 aaactagcta cattcccatg gaaaacagcg cttgtatagc cttgcttttg tccaaggatc
    30361 gctggtgcgg cttgaaagac attgtctgac ccaagttctg tgaataatga gccttgaggt
    30421 aaaccaaatg tgccagtttc caacatattt tcagcatcac ttgtctttcc ctgtcctact
    30481 tcatgaaaga aattgtcaaa gctgatcgtt gcttgattct ggaaaatact atttaaaaac
    30541 ggtgtgactt cttgtccgtc taccttcata ttaatcaaaa actgctggaa actttctaaa
    30601 tggagaacga tcacattttt ccctttagcg atcccaaatg tttcgggatt tggctctgca
    30661 taatgtttct ttgtataatc taaaacttca tcaatcccat tactgcttgc atgcgcgcga
    30721 acactgctag tcattcctgt tttgattccg tcataaatag tatacgcatc gattcctaga
    30781 tatttgacaa tatatgaacg gtcaaatgtt cgctgcagca attgtggacg gttggcttcg
    30841 ctcagcgcta gatttcctga aaaaataagc cctgaaagac aggtaacagc gatagccatc
    30901 ggtttgccga cagggttgct tttgataggt attttcttga ccttcaacca aaccagtagc
    30961 ccaatgaaca cagcgatatc cagccagtag atgacatcat ggggcttcat caaagaaaag
    31021 gagcttcctg acaatccttg cgacactttc gaaaagccta atacagattt gactgtaatg
    31081 aaatctgtga attcccgata aaaaataata ttcaagtaca acaaaagcgt atttaaaata
    31141 tcaatgatca aaagaatagc taacgctggc tttggctttt taatatacaa agaaaggcca
    31201 aaaaacaata aggtagtcgc aattgggttg atccacaaga taaagtactg gatcgtacct
    31261 tccacaccta atgaaaaatc tgcatagtag gcaatgatcg tcttcagcca gaaaaagaaa
    31321 acagctgtca aaacgattgc taaacgattt tggcacaatt ttttaagttg tgtcacgtat
    31381 atcacttctc tctattcaca ttttttctta ttttgctcct gaaaaatgaa aatttcctaa
    31441 aaaagttgtt atcttaagat ttttttattc tttctaaaga ttattccgtc cacttttcac
    31501 agcataaaac cgctgcgatg taagcgatta aactaatgaa gagataaaag tcttgtggtg
    31561 accgcttcac tccacggtat tcaaaagcca gtttctcggt attcgtacaa aaaaagaggc
    31621 tgtgacaaaa ttcttgtcgc agctccttat ttcttgatgc aggacagctt tttcacaact
    31681 tcttccttaa acggtgttct cctgccaact cctcggccac aagtcacaat atttataaaa
    31741 catgagaagc tgttttttga aattgttcct tgtggctcag agttaaacgg caggctcaag
    31801 acctcttgat acacggtgtt caatcagctg accctcggta ttagattatg tcaggacgct
    31861 aattttacaa cctcttggta aacggtgttc aaaagcttgc atctgcggta ataagcccaa
    31921 ccaaacgaaa aatataagga gctatttttt gaaattgctc cttgtggctc agagttaaac
    31981 ggcaggctca agacctcttg atacacggtg ttcaatcagc tgaccttcgg tattagctca
    32041 aaaaacggca aaatatgagg aactattttg ccgtttgttt ctctaatact caggtcaaaa
    32101 cgctgatttc acaacctctg tttttcattg tccataggat tgatattgtc cttgtgatga
    32161 ctcattcgta ataattgagg cttgattttc ttcacttccc gttatcgttt gtccactgcc
    32221 agaatctgaa ggacttgttc ctgtcgactc gtttttttga gaagagacag ttgttttctt
    32281 gacttgttca tttgtctttt tccattccgc taaacgatcg gcgaccactt ttttgtctac
    32341 tttttcagaa taagcggtga tcacttttcc atttttcaaa cgggctactt tgtcgccatc
    32401 atgagtcaaa agcaaaacgg tcaaatgctt gtctttaaat tgatattgtc tgagagaaat
    32461 cgcactaagt acgtcatcga tattaataaa ttgattttct tcttttcctt ttggcaaata
    32521 aatgtccaat tcgccttttt cgtaattggc agttatttta tactccgccc accaataagt
    32581 tgagaattct tttttgaaat tatctactga ttctttgctt gctacgattt tttctgtttc
    32641 tttctgagca agatctgctg tcatttttcc tgaaaatgct gcggataaaa taagcagtaa
    32701 gaaaaaaccg aataaccaaa agagaagaaa cggtcttttt ttcctcatgc tttcctccac
    32761 tcctatttaa ttttgactat tctattatat acaaaaaatg aaatagaaag acaaaaaagt
    32821 tgaaaaagat cgtaaataat tgaacttctt tttcacaaaa tggtaagaat ggagaatttc
    32881 acgcatttgc tctcaactat tctttcttaa gcctcaaatc cgtttttatc tgatacgtct
    32941 ttgaaccaat gacccgattt tttgattgtt ctttttccat cttgtgctaa gtctactgaa
    33001 ataaagccat atcgattctt atatgcattg gtccacgacc agttatccat acatgtccac
    33061 atatgatagc ctaaacaatt ggcaccttct tgaatggctt gatggacata tttcagatga
    33121 gattggacaa attcgatccg ataatcatcg tggatcatgc catcttcttt gataaatcgt
    33181 gcttcgtttt ctacacccat accgttttca gaaataaagc agtcgatatt tccataatga
    33241 tctcgtacat tgatcaaaat atcatagatt ccttgttcat agatctccca tccgcgataa
    33301 ggattcattt tacgaccagg catttcataa ttgtcgaaat aatcctcagg cattggaccg
    33361 tttttagtat cgattggtgt ttcttttgct tttacccggc gtggctggta ataattgata
    33421 cctaaaagat ctactgtatt ttctttgata accgtcaaat cctctgcatg gatcacaggc
    33481 atgagatcaa gttcttttac tatctcaatc aattcacttg gaaactcgcc ttttacagct
    33541 ggatcaagga atgaacgatt gaagaacgca tctgctattt gcgctgcttt aacatcttca
    33601 gggtttgatt cgtctcttgg atagcttggt gtcagattca agatgatacc aataacacca
    33661 ccttgctcca tttcatggta agatttgatg gctaacgctg aagccaacgc ttcatgatag
    33721 ccaacttgga tcgctttttt catatctgac tcttctggat aatgccatcg atataaataa
    33781 cctgcttcga ctggaacgat tggttcatta tgagtaaacc atttttttac tttcgaacca
    33841 aacaattcaa aacaactttt ggcatatttt tcataggctt caaccgtttc tcggttcaac
    33901 catccgcctt tttcttgcag atacatcggc atatcgaaat gatacagatt cacaaagggt
    33961 tcgataccgt tttcaatcaa ctcatcaaaa taacgatgat agaaacgaac ggcttcttcg
    34021 ttgattgttc cagattcatc aggtatcaaa cggctccatt ggatagatgt tcgaaatgaa
    34081 ttatgcccag tctgtttcat caactgtaca tcctcttgat acttcgtata aacttgcgag
    34141 gttttttcag gtcctacttg atcaaagaac ttttgcggtt cttttttgta ccaataatca
    34201 aaaatgttct ctccttttcc atcgcctgaa atccttcctt ctgtttgtgg gccacttgct
    34261 gcagaacccc accaaaaatt tgctggaaat tgatattttt tcatcaaagc acctcttttt
    34321 cttttttagt tcgttttagt tgattccatg tatgatattt tccatcacaa tcactttttc
    34381 attttcttcc tcttcttttt tgattcgttt taacagctgt tctgctgctc gttccccgat
    34441 tttatctgtt ggtggcgtga tcgtgatttt cggaaggata ctcagatcgt ttttatctaa
    34501 ataatcatag tgactgaacg caatatcctt cccgattctg acaccttctt gattgaaaca
    34561 ttcgatggca ccaatcgaca tattgtaatt gctgacaaaa atagcagttg cccctttttc
    34621 taataatgct tttgccccgt aatagccgct ttttctagaa tagtctcctg taaagacctc
    34681 taattggatc gatggatcat tttggaccgc ttctttggct cctgctaaac gttctcttgc
    34741 tacgtagtct gtttgagtgg cagcaataaa tccgattttt ttatgtcctt gttcaccatg
    34801 atgagcgatc acttttgcta cactttctcg atcattcact aatattgtat caatacctgc
    34861 tggtccttct ggacaattca atgcgataac tggaatactc atttttgtga gcatttcaaa
    34921 tccagaccac tcttcttcgg ataagaagac aatcagccca tcgatcacat gcttcatcag
    34981 gtattcaatt ttttgttcaa ttttttctgg ctgatcggca aacccgctta ataataagct
    35041 atacccattt tcttcagcta ctctttcgat ccctgagaca acttccgacc caaaacggct
    35101 ggaaatcccg tttatcaaaa gtccaataga gaagcttcga ttattcttca accctttagc
    35161 aatgatgttt tctttatagt ccaacgctga aatcgcttct gctatttttt tcatgttctt
    35221 ttccttcagt tgctgaccat tcaagtaacg ggaaactgtt cccactgaaa caccagccat
    35281 cttagccaca tcacggatcg atgccataca ttctcactcc tgccagttac atttgatttc
    35341 tctttcttgt tgttcgatta tgatgaccgc attctttgca tagtgttgct tgtcatcaat
    35401 atccatataa ctaaagtctt cagtagagca acttcctctc actattaatt atacttaaac
    35461 aagtatttga accggttcat ttttaatata gcattttttt aaaagattgc aacacttttt
    35521 taaaacgctt tctgaaagtg gcggattttt tgccttttta aaattttatt ctaaaattta
    35581 ctctatttgt gggtgttttg tgcgtttttc tcttccatcc gtttgaagtt ttacattcca
    35641 atggtataat ttctttatcc tatcaaatgg gggaagtatt ttgaagaaat taatcgtact
    35701 atttattttg tctgcgggac ttttgacatt gactggctgt gatttgtttg gcggctctga
    35761 ccaatcctcg gacgaaacaa ccgattcttc tgtcttaaaa gcggaaaaag cagataccac
    35821 caacagtgta tatacgctaa aagtacgtaa agatatggat ttatccgcgg gacagttgac
    35881 atttacagat aaaaaaatgg aatggacacg cacgtatcag acacaaggaa ctgattcttc
    35941 tgaatcatct gaagaaaaaa cagtgctgac agatatcaag atcactacca aagatgacga
    36001 atatattatt tcaggaaaag aaaataaaaa agaaacaaca gtcatattta caaaaatcgg
    36061 taattaccgt ttgaaggatg aggatggcaa catctacagt ctatagtgcc gtctgttgga
    36121 tcttcttttg tttctcccct tcttttctgc tatagtaaag aagcaagatg attgtaaagg
    36181 agacgaaatg gatgatcgat agtcctgatt attgtacatg taataacaac aaaaatatcc
    36241 atacagaatt taatgaccaa ggaagcttga gcatatgcga caactgtcac aaactagtca
    36301 aagattcatt taaaacattt gaatcttatc ctgttcaaaa ttatgtaaaa agtagaagaa
    36361 gataaacaaa tccaagcaaa actcactatg gctgattttt atgaacttca aactcagctt
    36421 cttcctttga tcaagcttca tcagtaaaac aaacagtgga tcatttcagt ttacaaatca
    36481 aagagtaaag atttatttat ttcaaatatt tgaatataaa ataaccaaac gatagaaagg
    36541 cttattctac atcacgtagt aatttttctt tcccgtcgtt tggtttttta tttttagcct
    36601 aattcttgac cttttgcatg ctggttatcc gtaaaatatt ttccagcagg ctttccttcc
    36661 aattcagcta atttttcttg gatctgacgg atcacctttt ttgcatcttc tggattttct
    36721 acaaaatcga atttatcccc atccacttgt attttaggac atacatcaaa atcttcatac
    36781 cattgattat agcgggtgtt caacgtttca taataggtat ataacgtctc atcgctttct
    36841 agctgttcat agtcacgccc acgttttttg atacgttcta gcatcgtgtc caaagaaact
    36901 ttgatatgga ccatcaaatc aggccgtttt tttggagcaa catcatctaa ctcgtttagc
    36961 atcgtatcta ataagctatc gtactgctgt acttcgatat ccgttacccg acctaaatca
    37021 gcgtttagat ggaacaacag gctatcttca tagatggatc ggtctagcac attgtcatcg
    37081 ttcactaaag catttttcat tgctaaaaat cgcttgttca aaaagaagat ttgcaataaa
    37141 aaagcgtact gctcaggatt tgaataaaag agcggcaata cttcgttatc ttcgacattt
    37201 tcataaaatg gtttagaatc aatctctttt gcaagcatat ccgtcaaact gctcttacct
    37261 gcaccaatcg ttcctgaaat caaaatcact gacatacaac ccttcctctt ctataagtac
    37321 tcgcctacgt aagtttcaac aggcaatgtt tcaaaatcat attcacgtac agcttggtct
    37381 gattttaaat acgtatggag actttgtgta atcatcaagt tcgtaggtac cgtctcttct
    37441 tgtacaatga tcatcggttt tttcaacata gttgccacac ctaattcgta agccgttcct
    37501 ggatcgattg tttgatgctc aaagtcaagg atagcaacaa caaattctgc atttgtcagt
    37561 tcttccatat ctttttcgta tacttcctgc gcccatccag cgctgaatag ttcataattg
    37621 ctttcttggt gatgacgtgg agagtagaaa cttgtgactg ttttgttttc ctccaacgct
    37681 ttttcgatac gacttactcg atcgatttgc tcctctgaaa aaaatggtcc tgctaaatat
    37741 atttgactca ttattttgcc tccttcaata ataactgatt tttaatagat actgctcgta
    37801 attcattgac cagttccaat ggattgttgg aaaagattac ttgatccaac cattttttat
    37861 ccataaaatc ctcttttgca aaagtttcta ataattgttg caagccgctg taataaccgc
    37921 caatattcaa tattccgatt ggcttgtcgg gcgtcagacc gattgctatc caagaaagaa
    37981 gttgtgagaa ttcctccaat gtacctagac ctccagggaa aacgaggaca gcatctccct
    38041 tttcaaataa taataccttt ctttcatcga tcgtttctgt aggaataaat gtagtaacat
    38101 cttttccagg caattcagtt tcaaaaagcc cattcggata gatacctgtg acttttccgt
    38161 tattttccag tactgtttta ctaatcactc ccataatccc ggagtatgaa ccaccgtaaa
    38221 ccagttcgat ttcttcttgt gccatgtatt ttcctaacgt ttgcgcaata aatttgtaac
    38281 tttctttatt accaaaacgg gaaccgcaaa aaacagtaag tttcataaaa aaacactccc
    38341 taactcaaaa gtctatcttc attctacacg tgttttttta aaataaaaga ctagattttg
    38401 tagtttttta gtggtatata ggatagacgc cactatatat agtatttaga aaaaaatcat
    38461 ttttcagcca ttttatagat aaaacaaaaa taaataacgt gttttcatcg atatgaaatg
    38521 acttacttat agaagatact gggatttcgt cctatatgcc aataacgtgt attattttaa
    38581 acaacaaata cttgtttctt ttattaacga aaaaatcatt ttgttctcgt gttatgtttc
    38641 actgataaat gatgtttctc taaataaaaa agccgacgaa ctaaacgatc aaaatttgat
    38701 catttttgtt catcagcttc tcctaaaaat ctttttgtta acgataaata gctggatttg
    38761 taaaaatatc acttgaatcg tcactgctgg aagaaaattc agtgataacc gcacctgttg
    38821 cgcctgcctg gaactagtgt ttgatattgg gctcgaacgt gtactgatct ctagcatgaa
    38881 gaataatttc tttttttact gtataataac tttctttcct ttttggaata gtacaggaaa
    38941 gtttttttgt ttcctttcct tcgacataag gtataccaga ccctttcgac aacgaaaggt
    39001 tctcattttt ccttcatcgt catttcaagg agggtgccga tgctctggac agtttcgatc
    39061 gctcctaaac caaaatctgt atattcgatt ctttctaact ctgattcttt cataccattc
    39121 ctgtctcaag gtacatctat tgtacttttt tcttgtaatc cattctgcct ctcctaacta
    39181 attgtcaaat catcccaaga tatgtcttct tcctcttttt cttcttggat gatttctgca
    39241 ttcggatcta ctggtttttt caataataaa atggttattc ctgtgacaaa agaaccagct
    39301 aacaatgcac caatgaatgc ccatggtctg gtcatcgtag cgactgcaaa cattcccgat
    39361 gccggaatac ggctatctgc gccccaaaac atgcttaatc cgccacctac tgtcccaccg
    39421 actcccgttg ctatgacatt gcgtgccaag tcgttcaata cgatagggaa acatccctct
    39481 gtgatctgac agatccccat aggaaaagcg gtcttcaatg tatcgatttc ttgacgattg
    39541 agtatatttt tacgaatgat tttccctaag aaataagcga tcgtcaagcc gaatggggtt
    39601 accatcgaag ctaaaatcaa tacagtgatt ggttcataga ttccttctga aaatcgtgcc
    39661 acaccccctt atccagaata aatggtaatc cagaaacagc cgtgtatctg cggtaataag
    39721 cctaaccaaa cgaaaaatat gagaagctat ttttgtttgg ctgttcttat tacttgatgc
    39781 agggcagctt tttcacaacc tcttttttta tttatgtttc ataatcaata tggtaaggat
    39841 gcagctccgt atggtagacc gcctccgaat attcaatcat ttccccaatt tgcgtagacg
    39901 atttccgtat ccgtttgatg ccgatctgtg cgtctgtttt cagaattcct tgttcttctt
    39961 ctgttaaaaa tactgccgag aaatcatcag aaaatgagtc aatagtatat ccttttttcg
    40021 tcagcaaacg atacaaagat tctttgccaa attcttctct gctgaattgc atcatttctt
    40081 gtggaagata atgaacaaaa taaatataag gctgattgtt caaataatat aagcgcttaa
    40141 aacaaacagc ttgctcaccg aaaaaggaaa acaaaggatc ttccttcttt atatggacca
    40201 attgcaaatc cagcgtttct ttccggactt catatcggga gttctccaag atttgcgtaa
    40261 agcttgccgc tttggataat cgattgtaag gacgattgct tagaaccgtc gtcccacgac
    40321 cacttttctt tgcgacatat tcatctgatg ccagcagttc gattgccttt cggacagtga
    40381 ttttgctgac ttgaaacagt tcttctaatt cactttctgt cggcaacatc gaaccgactg
    40441 gatattttcc tgagaaaata tcttctttaa ttttatctgc gacttccaag tatagtacac
    40501 gtgtcctttt cataacgtca ccctttgctt tactattact aaaagaatag cacataaaaa
    40561 ataaaaaaag aacacaaatc acatgtgaga agtgtgttct ttttcattca gctgctttaa
    40621 ttacaagttt acgccatttg aggcaattac ttctttatac caatcaaaag attttttctt
    40681 tgatcgattc agtgtcccat ttccttcatc gtctagatcc acatagataa agccgtaacg
    40741 tttactcatt tctcctgttg atgcactgac aagatcgata catccccaag gcgtatagcc
    40801 catcaaatct acaccgtctg ctactgcatt agccatcgct tcgatatgac gacgtaaata
    40861 atcgatccga taatcatcaa taatcgtgcc atcctcttcc atatgatcaa tggctccaag
    40921 accgttttca acaatgaata atggtttctg gtaacgattg tataattcat tcaatgcgat
    40981 tctcagccct tctggatcga tctgccagcc ccattcactt gcctcaagaa acggattttt
    41041 tactcctccc agcaagttcc cttctgaagt tgctgcttgt ggatcagtct cattgattgc
    41101 cgtagacata taataactaa atccgatgta gtccactgga tattctttta gcaactggaa
    41161 gtctttttcc gtttgctcta atttttccgg atcgactcca tattttttat gggtacgcgc
    41221 tgtgtatgca gggtattctc cacgtacttg gacatccgtg cagaaaaaat tgaattcttg
    41281 gttttggatc attgttgctg cctgattgac tggattggca tcgatgctgt aagttgttgc
    41341 atagataatc atgcagccga tttggatatc cgaacgcaat tcgtggccga tttttacggc
    41401 tttgctactt gcaacgaatt gattatgcca tgcttggaaa atattttggt actctcctgc
    41461 tccattgctt tttactaatc cttgacttaa agctggaaag tgaaaggccg aattgatttc
    41521 attgaaagtc atccaatatt tgactttact gctgtatctc tctaatactg tctgagcaaa
    41581 tcgttcatag aagtcgatca gttttcgatt tttccagcca ccgtattctt ttgccaaatg
    41641 aagaggcatt tcgtaatgag aaatcgtgat cacaggttca atatcatatt ttaagcactc
    41701 atcaatcagc tgatcataaa attctaatcc cgcttcgtta ggagtacctt cgtctccatt
    41761 tggaaaaatt cgtgtccagg caatcgaaaa acgataacat ttgaatccca tttccgcaaa
    41821 caaggcaaga tcttctttga aacgatcgta atgatcgatt cctcgatgat tcgggtaaat
    41881 atatttttct tgatcaatcg tccagtcaaa ttcttcactg ccgataatcg caaagcgctg
    41941 cttacctcca ggcatagcat ctgcaacgga tagtcctttt ccgtctcgat cataagcccc
    42001 ttctaattga tttggcagca gttgctccac cccataaaaa tttttctgaa aaacgtgatt
    42061 gcatcctgat ttcctcctta ttttacaatt gccagaacat cttcgtttgc aataatctct
    42121 ttttggtcta attcaagtat atcggcaaat gcatcagtat tcgtaatgac aactggagtg
    42181 attacgctat agccagctgc tctaattgct tcgatgtcaa aacgaatcaa taaatctcct
    42241 gctttgacga gatccccttg tttgactaag atctcaaagc cattcccatt taattctact
    42301 gtatccatcc cgatatgaat caaaatctcc gctccgtcag tcgttgtgat ccctacagca
    42361 tgtccagttg gaaaaatagt cgtgatctct ccgtctgcag gtgcaactag ttctcctact
    42421 tctggatcaa tagcgatacc ttttcccatc acgccagaag aaaaaacttg gtctggtact
    42481 tcactcaatg gcagtacttt acccgttaaa ggacttttta aggtatgccg tgctgaagta
    42541 atcggttttc ctgcattcgc atgtttgttt tctaattgat tgtcttgtgt ttgttcgtca
    42601 aatcctaaaa tcaatgtacc tacgaatgca agaacaaaag caattcctgt agctattact
    42661 gctactgtca catttgactc tacaccatcc accgtattaa tgaaagtcgg aatcgtcaat
    42721 aagctgacca aactgctgga gaaagctttt actccggaaa atccgataat agctccacca
    42781 atacctccag aaatacaagc ggcaatgaat ggtttcttca aaggtaatgt cacaccatac
    42841 acagttggtt cagtgattcc aaataacgaa gtcactgtag aagaaatccc taatgctcga
    42901 agtttcgtat cttttgttcg caatgctaca gctaaagcag ccccaccttg tgccaagatg
    42961 gctggaagaa gcattggcat caatacatca aacccatatt gttcgatatt caaaaacatg
    43021 atcggtacga atccccaatg catgccgaac atcacgaata cttgccataa acttcccatg
    43081 attagacctg ctacgattgg actaaatcca taaatcgagt tgaagagacc gcctaaagca
    43141 ttaccggcaa ccgtcccgat tggtcctaaa gcaagaaatg ttaatggaac catcactact
    43201 aaaacgacaa gcggaactaa aatcatttgc agaaattgcg gaatcacttt ttttacgaat
    43261 ggctctaatt tactttgaac ccatacggct agaatgattg gtatgacaga agaagtataa
    43321 ccagatggtg aaagaatcac tgggatccca aagaaactga tagcttctcc cgcaccagca
    43381 actgctgtga tagctggata cacaagtgcc atagcaattg ctactgctaa aaactggttg
    43441 gctttgaact ttttagctgc tgtaaaagca agcatgacag gtaagaaggt aaagacacca
    43501 tctgctgcag caaacaatat ttgataagta ccgctatcag ctgtcagcca gcccatcacc
    43561 gttgccaatg ataagaatcc tttcaatacc ccggcggctg ccatcgcccc tagaaaaggt
    43621 gtaaatatac tagagataat atcgatcaga cgattcaaaa tatttttgga ctcttctttt
    43681 ccatcctccg aaactgcaag accttcctca tccacgatcg attgataaac atctgctaca
    43741 ttactgccaa tcactacttg atactgaccg ccactttgta cgacttggat cacgtccggg
    43801 tcttctttta acttttgcgt atctgctaaa gattcatctt ttaatctgaa tcgcaaccgc
    43861 gtagcacaat gtactaaact tttgacattc ttttgtcctc ctaccgcttc ccatacacga
    43921 cgaccaactg cttgattatc cattgttttt tccttctttc ttttttattt aaaaaataaa
    43981 aacccagatt gaaaaaaaca gtcacaaaca ttcgtgaacc tattttttca atccaggttt
    44041 tgcctgccat acagtaacaa tccttatgat tggatgcttt ttttctcact tactactctt
    44101 tgaatatgaa tcgttaaata aatctgctct gcgatcccca tttcctgttt ttgcgtttgt
    44161 cgaagatact cattgatccg ttcgacacaa tgaaacgctt ttggatactt tccttgaact
    44221 aatgcgtaga gaaattcatc ttgctcgtca gatccttccc ctttgagata acgctgggca
    44281 aaaaactgca gatgagtaat gatacgctga taattcaaag attcttcatc gaacacgatg
    44341 ccgaagaatt tactgatgat actcagtata tctcttacca ttgtggtagc ggtcatcgtc
    44401 acttgcatgt cttcgtttcc ttgttggcta ttcacaaaat gaaaggcaat aaaaccggct
    44461 tcatcctcag aaagttccac acccgtcttt tgtttcacga acaaaagagc ctctttcgct
    44521 gcttgatatt ctttcggata aaattttttt gtttcaaata ataatggatt aggcagttgg
    44581 atattttctt ttgctcgtgt gatcgcataa tgaagatgat cggtcaatgt cagataaata
    44641 ttaggcgaaa attcaacttt tagtttatct tcagccagtt cgactacttg tttgacccat
    44701 tcgatctcct ctgccggaat atcagccatc agctgctgga attggttttt agcaaaagaa
    44761 tctcttaaaa caaattcttt ttggaccgct tctggcgaaa tctcgtcccc aatctttttt
    44821 tgaaaagcta gtccttttcc catacaaacg acttcttccc cgaatccatt tttcgaaatg
    44881 acgacattgt tattcaaaat tttatcaatt ttcattttgc ttcgccctct caagaaaaat
    44941 aaaaaaacct agaattcgcc tactgtttct tagctgaatt ctggttttgc ctgccgtaca
    45001 gtaacaatcc atttcttatt caaaatcatc ataccatcga atttttattt atgcaagcct
    45061 tttcatataa aaaataaaaa aactggaaca caagtctatc tagaaaacaa ccaaatcaga
    45121 gatgcacctc tttgcgtttc tctttttttc agcaaattgg tttttcctta tcgattcata
    45181 tcctttggct gtttttaatc tgtaacgact tatgtcccag tatcctaaaa tgtttttgtc
    45241 tctagattga aagcagatct catatcatct gtcgcatctt ttatcgatgt aacactttcc
    45301 agttctcctt gtacaactag tcgcgtgacg gcgtccatgt cgccacaagt aattagtgtg
    45361 acaatttctt ttccctcgac ttcattcagt aattgggtat ctgtaggagc aactttttct
    45421 ttaaaaaatg ttttgtatgt atacacattt tctaaatctg tcaaatagat cttggcaccc
    45481 atctctaaat tttctagcgg tgtaaacagc aattcagggt tcgttgcacg atggcttgct
    45541 aaggcataat ttccttttcc catttcttga tctggagata atgttcctgc tccgtaaagt
    45601 agtgacgtat tatccaagcc tttgaaaacc ggtaaattga ttgaaactga agggactgcg
    45661 atactagcaa tcactggtag atctgttcct ctcatttgcg acctcatgac cccttctgtc
    45721 gtcattggtt ctaccgcatc aaagtcaaaa ctgacatctt tatcgttatt ctttttcaga
    45781 tcttcttttg ttacattcgc tatagcatat ttatctcctg tctctctgac aaaataatcc
    45841 ttgatctgtt cattgaaaat caaagctagt cctactaata acaagaggaa caagaggata
    45901 ttgactagcc atctttttgc ttttcctttt tttttcattt tctctccctc cagactcatt
    45961 ttactgtttt tcttttgctc tttccaacag ccgaatgaca catttctaca actctgtgta
    46021 tttttttcgt acacctgcgc ttttttctac aggatatttc aaattatttt tgatcaattt
    46081 ttcttcaatg atctcatcta gatccaggtg aaggttgtct gccatcatgt atgaatagat
    46141 caatacatct gccagttctt ctttgattcg ttctaattgt gtttctttta cttcttccgg
    46201 cgttttccat tgaaaaagtt ctaacaattc agatgcttcc aaagaaatag agatagccaa
    46261 atctttttcg ttatgaaatt gtcgccaatt acgttcatct ctaaactggt tgactttttc
    46321 catactattc atttgcttgc cttcttccaa tcacttctta cttgattaat cataccattt
    46381 ttttacataa aaaatagatg atgcttttaa aaaaactcct gttttctttt atttaaaata
    46441 aatgtcatag aaaatcaata atttaggtag ccaccatgtg tatgcagtag cgtgtcttct
    46501 tcccttgagg ctttttgttt ttcgatacgt tcttcttccc aaacagacaa cgctactaga
    46561 atctctctta atgattttcc ttcttcggtg agcgagtact ctactttagg cgggacttgg
    46621 ttataggctt ttcgatggat actttcatct tgttctaact ctcttaactg ctgggtcagc
    46681 attttttttg tgatcgtagg gatttgcttg cttctccacg cctaaaataa gcgatattgc
    46741 tcttcccttt atctggtgta agcaaagcag ttagcatttt gacgatcgtg ggttccgctt
    46801 ttcacttctt atctacttag tatcatgaag tatactttcg tactaatact aagtagcgga
    46861 agtgtcaact actttgttta actacatagc aaaagaaaca gaagggtgac aacggtcatc
    46921 acgcttctgt ttcttttgaa tatgctgtgt tgttgtctct atctccgtgt agactttcct
    46981 caaacttatc ctttgacagt aagtcagtag gctgatttgc tctttctaga tgccagatac
    47041 atttctacaa aaaaatagtg attctgtttt gaaaattcga tttttagttc tgcattttcg
    47101 taaacatcca acattttttt cgcactgtat agacctaacc cgccattttt tttagaggtt
    47161 tttcctcttt tgaccatctc cgatagcgtc aaattcgttg gatttgcctt gttcgctata
    47221 ctaaaacgtg atccctcttt ttcttccagc agattgatcg taatcggact ctgatcggct
    47281 acacgatgat cgaatgcgtt agaaataaaa atagataaac agcgaataaa aacaaccaga
    47341 tcaatcggga ttgttttcac ttcgacagga ataactactt ggatcgggat tttctccgat
    47401 tctgcttttt ctagaaattt taaaaagaca cccctgacag ctggggatac aatatttgtt
    47461 agctgttgta tttgcggttt ttcaaaaata gctgcagaat gatcttctaa tatttctaaa
    47521 aattccaacg tttctttttt attttcctga agagtaattt ttaatgaaaa cagtagactt
    47581 ttataatcat gtttgaattc ctctagatga tggtaatatt cttcatttat ttgatacagt
    47641 tgaagcgatt cctgatgttg tctttctttt acttggaaac tgatcaacat aatggaaaaa
    47701 aacaaagaaa aaaaactgat gactatcgac aaaagagata gaagcagata attagatgag
    47761 cggaagtgaa cacttcccaa taccaagtaa atcacgcccc agcttaataa aatacaaata
    47821 cctccaaaat agtgttcagt tggataagta aaaagcaagt tgagaaatct cgtctgtttg
    47881 tctatacttt ttactaatag atagaaaata aatgcgataa gtagtgcaaa aaaacttgct
    47941 gttccaagat gtctctttcc taaataatcc atcagatcaa aaaccagaaa aaagctgagt
    48001 ccagtgatag caaaaaaaag attactgatc aaacagctga taaacaggtt tttcgtagta
    48061 aaataaaaaa gaaacatcaa taataagcca actaggtaag gaaagctcac tttagggata
    48121 ggaagtaaaa gtgtcaccac catcaaagct atacaaccaa agctgttttt tactagcagt
    48181 tgtttcgaaa agaataaata gatacctaaa tctaaaatca aagtaaaggt caaataaagt
    48241 gtcactgttg cgaccatctt attcctccat tgttttgttt caaaatgtac acatattttt
    48301 ttaataggag cctgcatatg aaaaagtatt ttatcataga agataaccag tttcacagcg
    48361 aatggatcat cagagaattg gtcaatcatc atatcgagaa tatcgtgcag ctcacttcta
    48421 tctctcaaat caatcagttg atccagttgg gcactattga taatcacgat gttttcttta
    48481 tcgatatcga cttgaagcaa tcactcacag gtattgatgt cgccgaacaa atacgtaaaa
    48541 aaaatgcaca tagtaatatt gtttttttca ccgcttatac gaattttgca gtagagatca
    48601 tcaatcggaa aatccttccg attggatata tcagaaaaga caggaattta tctgaacaaa
    48661 ttcgtcaagt gctagaacag atcaattgga aagaacagca gatcgtaaat gtcgataaaa
    48721 tctttattga tcaaaaaggt ggaagtctcg tcctgtatcc tcgggaaatt ttatatattg
    48781 aaagtatcaa aggtttgaaa aaccaagtta tggtcaaaac aactacagaa gataaattag
    48841 tgaatacttc tttaaaacaa ttgaagacag agttagaaaa gtatgcttac tttttacttt
    48901 taaaatctta ctgtatcaac ttatcgcaat tgcagaaaat tgattctgct cattatgtgt
    48961 tagaattttt caacggtgac tccttactcg ttggtcgaaa gatctttgaa aagacaaagg
    49021 aacgtttcca tgacttccaa aaaactgctt cgagttgatg aataagagct gagacgtaag
    49081 catagcagat ttcagggagt gggccttgaa tcggctccac tccctgaaat cgttgatatg
    49141 attttacgct tcctcttctg taggagcatc tgttaacgac catgtgaatg ttgaaacgta
    49201 gttgtctaca gcataattgg atacaggaac aaataattcc actgattgtc tcccttgttc
    49261 cacattctct cctaatgaat aaatccatgt gccaatccct tcatttgctc ctgcagttac
    49321 tagatctact gcctgatctg ctgggatttc gattccttca gcgccagttc cagaaacggt
    49381 agatggcatt ccactgctat tagttgcgct atttcgaatc acggcgtttt tgaatttcac
    49441 ctttgaacca gttagtttat tcgtatcaga tgttgtacca ataaattctg ttcgtttgac
    49501 tgataatttc caaccggcat aatttccgct tttatccgtt acttgtacga aattctgtac
    49561 ttgtgtttgt gctccagtac tgtccgttcc gctttgtaat gcagcatata ctgtttttgc
    49621 tgtacctaac ttgattgttc caaaatcaag atctggagct tggtcaatcg tcagtaatcc
    49681 ggatgttccc tgatttggtt cgtcaggtgt aaaaggagta tcaggatcat ttggatcaac
    49741 aggaggaact ggaacaacgt cttcaggatt tgcagcatct ggtttggtca cagaaatcgt
    49801 tccttctgta gtggctgagc tcttattctc agcaaaaaca ctttgtgtac ataataatag
    49861 acttccagct agaacggtac ttaatgcaat tgtttttttc atttcttttt ccttctttct
    49921 tttaaaataa ataatccagc aaataagcca ataagaatga ctccaacaag tatcagccat
    49981 cgccagtcaa atgtttctgg ctgatttttt aaatcaatat ccttttcatt caattggtcc
    50041 gctgcatcat cagttatttc aaaatcggta tggagattcc agcgtttttc tttccaagca
    50101 cccttcacaa ccgcttgata atgtcccgga gctaattttt ttccttctaa tgggacaagt
    50161 aaatcaaaat ttgtattggg tgctacatct attttttctt tttttattga aaggtaaggc
    50221 tcttttgttt cttttttgta gatttcaacc gatgtagtca agccgaacat ataagaagca
    50281 tttggatttt gaagattcgc cttaatcaca tttcttccat cgctttgatc cgcataagct
    50341 ttttttaatt tcaaatctgg ggaagactct ttttcgctat ttcaaatcaa gattgctttc
    50401 acttgagaaa actgattgac gacaccaact gtacttttgt tagaaacgtt ctcttttttt
    50461 tcttgaaacg tgatccctcc tgcgaccaca ccagccagct ctttttctgg cgctttcagt
    50521 gaaagtgtct cagtgaccgc actttgagga ggaacagtta tttctcccac tgtttttgct
    50581 aagtttttca tatcataact taatgtttca tcaaatttac tttttgattc tccatattcg
    50641 atcacaccgt ttgaattcgt cgtagcggag tggatcaatg tctcaactgt tactgattta
    50701 tctgttcggt taaagaccgt gtaggggatt tcctgtgttt tcccaggctc catccttaca
    50761 tttatatatg ttttgttctt atcaatctga ttagccggta attcaaaatc taccgcaaaa
    50821 ttgaattcgg aggcatggac aggtgtctgc caaaggatga atccaaggag aaggatcata
    50881 agtaaagagc agttgtttcg atgactttta ttccatattc gattcatcaa tcatcctcct
    50941 tgatctttat tctgtcggcg catctgctaa tgtccatgtt atctctgctg agtaattacc
    51001 agctatctga ttttctttgg ttaagacaag tttaaacgcc cgaccgttat ttaaatattc
    51061 tgaataatct gcttgcacat tcgttgaatt ttgtcctctg acaatttcca gttcttcttc
    51121 attcactaat tgatactcct ctcctttctt gaaatatagg acatcctgca tagaaaatcc
    51181 tgcatcattt gtgaaaggct gagataattt tatcgttaag atccacggat ttttcgtttc
    51241 tcgctgatca taaatccata atttttcctg tatatccatt tggccaaatg cttcttgtgt
    51301 caaaccagtt gctttgatct gaccaaaatc gagtttggaa ggggcaagaa atgagagacc
    51361 accttctact acgatcttcg tagcaggtgc aaaaggcggg gcatcatgat aaacaactgg
    51421 cgtatcactt ggattgtgat tcccaatttc atcgttggta cttggcttgt tctgcacacc
    51481 cttagcagta gcttctcctg cttggtcgtt taatacaatt tctaatacat ctcctgcaaa
    51541 aagttgattt tctgggatag aaagaacaaa ttcccctgta tcatcaatag ttgtttctga
    51601 caaaacagct tgattttttt tcaatgaaac caaggctcct gcttcacctg tacctgtgat
    51661 cgtgcgagaa gctgcattga tcaatgtggt acttaaatta gcaggttcag gaggaatcac
    51721 atcgactgtc gttttggcag agtccagttc tgtcacttct tggccacctc ttactttttt
    51781 agcagcaaca acttgcactt gatcctccgt ttttaatctc tcatcattgg gtacttgaac
    51841 ttcgaacaag ccgccttgtt cgccttctcc ccatgggtta tatcctgccc cttcattttc
    51901 atctgtttgt ccttgcgtga gagcagtaat ttctacagat gatttcgtcg tatctgcgta
    51961 agtaatacgt aaagtcactt ctacttcgcc tgtccaagca tctcttggct ttgcctcgac
    52021 accttcggga acaacgacat gcccatagat ttttttatct gcattcgttg gcactcttaa
    52081 ggattcaatc atcgggagct gattattggc actgattcga ttataatttt tcattccggc
    52141 acctgtctga gcaaattcat ttgttaaacc tgcactaggg cttgatacga aattgcctaa
    52201 gtcggtccca ttgatcgata agtctgtcat atcagaaaaa taatccgggt ctttttcaaa
    52261 gttatcgaat gttgcgcctg tttttcgcca taaagcgatt tctgaatggg cgatctttaa
    52321 agaagaatct ccatttcctt ggaataccca gccgcctatg ccattggtct cgcttccaat
    52381 ccgtttgttt tggaaatcaa aataggtagg agattctaag tccacattca acacatcccc
    52441 actgatgatg ccggtcttat cttcccctgt tttcccgacc atgactaaat aagttttttc
    52501 acctgccgta aaatttaaat caatatctcc agttgtatct acagttgctc cggaatctgc
    52561 gcgtatatcg atatttgatt tttctccagt gactgtaaaa tcagctgaac ctggactttc
    52621 tagaaaacca gaagcgtcat aaaattggat cgcttgattt ccttgattga gtctgttcac
    52681 gtaagatttg ccatcacctg tatttcttac cctcaatgag cctccattgg ttactgtcag
    52741 cttttgcgta ccattctcaa aacgaactgc gctggaacgc ccactctgct tatcgatgtc
    52801 gataactgat ccggctcctt ctacgatcaa agaaaggcct tttcctacaa aacgcatccc
    52861 attgttcccg ggttgatcgt catctccagc cattttgacc gagatttgtc caccacttga
    52921 tacatggatt tgactagaat ctccttgcaa gcgaaatccg cccgtagttg ctgtattgat
    52981 ggtgattttc ccaccattcg atacagtgac tttcgatcct gaacctctca tttggatgat
    53041 tccgcgattg ttgtcattgg taatattgat ctttgtattt accgaaaatt cgcttcccga
    53101 accactgact tcaaacatga cattaccagg tagcgtatcg actgcctctc cttcatcgct
    53161 aacgccatct gcccgatcat aatcaagaga gacctttgaa ccgcttaaga tatggattcc
    53221 tggcgctgtt tttccactac cagtctcatc tcctgaatct agaaaaccgt agaatttccc
    53281 tagatacggt ccataaagtt gactaccatt gatcacattc aattgatagc ttctacaaaa
    53341 ataggcttta cccggagcgg cattcagtcc ttccttcact ggttggacat ccaacacacc
    53401 ggaaacacca tctaatgtga cagttgcacg aggagcgtaa atcagtccta atttggaccc
    53461 atcatacaaa tcaagtgtcc cggtaaattt tatttctccc gtaaaatgat atctgcttcc
    53521 ccaaaaatcg gtcatataag caatgataat ggcatttcca tcgatatttg gtgtcaatcc
    53581 acctggatgt gcagctgttc ctttaaaatt ctggaactcg atcgactgag gtatctcatt
    53641 gatataaata tggtgtgtgc cgaaattgtg agcgaatcca ccaaaatcaa taacgatatc
    53701 tcgtttgatc tctaattccc tgtctaaagt caaaggctga atcagtcgga tcttagttac
    53761 tgattcgtct gcgagaagct gcgccagttc ttctgttgtg gcagcttcgc tgtacccatc
    53821 cgctgaaaca gctcttgcca gtgccgcttc actgctatca gacgtttgtt cagctacttc
    53881 tgaggtctca cttgtcttat tttcttcttg ccttttatta gtagaagcct cactttgaga
    53941 ttctgtagtt tcagaagtaa tcgattcttc tgaaaaagta gtcttgtcaa aactttgatt
    54001 ctctgattgc gttgattcgt ttgttttctc tacatcggca aaaacggatg tcaccatcaa
    54061 gagagggaag acaaacaaaa tcgacgcaat cagctttttt acacattgat tcattctcca
    54121 ccatcctctc tgacgttttg acaattttat tttatcgttt taatctattt ccaattgtac
    54181 tttcatattt aaaatatgta tttttatgga aaaatttttc tattaggtgt aaatttcttt
    54241 tttgtacaaa aaaaaggctg tgacaaaagt cttgtcacag tcccttatcc cgaacaagcg
    54301 gtgttctccc gccaactcct cggtcacaac ctccatttta acaataaacc tgtaaatgtg
    54361 agggcaatac tttgattgtc acaggtaatt tatccccttc atctccatca acattcgtcg
    54421 tcaattcttc ctcatcagct aatgacacaa ctaattgctc acaagtaagg taggctagat
    54481 ttttcgtaga ttcatctact ccttttaata ggtctggcaa tgttttaagc gtatcgatca
    54541 aagaagaatc ttttaaataa accaaatgga gcttaccatc atccactttt gcatttggaa
    54601 gtaatgtttc aaaaccgccg actgagttag ttagtcctat cagcaaggta ctgctctcaa
    54661 tttcttcctt ctttccatca acttctacgt gaaacgagta ggattgtgtg gaagctaact
    54721 gtttgattcc tgacatgaaa taagctagtt ttccaaattt cgtcttcttc tccgcatcta
    54781 catcattgat tgattcaggg atagagccaa ttgcgacgac attcataaaa tagtcgtcat
    54841 ttatttttcc aatatccaat ggcttgactg attcaatcga aaaatgattg atcgcttcct
    54901 caggttctag cggtatccct aatgctcgag ccaagtcatt caccgtaccc aatgggaaaa
    54961 atccgaaatt tggccgatgt tcttgttcag cgattccact gattccttcg ttgactgttc
    55021 catctccgcc catgacaaac acactatgat acccttccgt agccgcttcg cgagtaaagt
    55081 tttttgcatc ccctgctttt tttgtatgca agacgaccac ttcatcaaac accgattcca
    55141 attttgctat cgctaattgc tcaaattcct ttgcttgttc accgcctgaa ctggggttta
    55201 ccactaataa aacttttttc atcactttct ttcccttctg attgatttct ttaatataac
    55261 gatagcataa agaaaaaaat taggctgtga tgaagtactg ttcatgaaac attcatttat
    55321 tttttgacaa gataaaacat ccatactatg aaactgagga aaattaaaac aatgatatag
    55381 gaaataaaag aaaatgatag gcattgtata cctaataaag taaaaatctt aggtggcaga
    55441 taaaaacaca aaaatagcaa gagaatgtaa tagcgtttct tccacttttt gttatcagac
    55501 cttatagcga tcagaggaat accaaatgca taactgaaaa actcttgatg tactgttaat
    55561 atgtcacggc cttgaataat aaatgcgaca atccccgcta taattagtgt gcctaacacc
    55621 aaaacggaaa attgatgaat atattttata tacaaatgat ccattgtact tcctgccttt
    55681 ttatttgtga gaaaaacaaa tccgtatacg tgaataactt ctagaaataa aagaaatttg
    55741 caaaaccact ccatttatct acttctacgt gcattttttg atattgtttt ctttatttaa
    55801 gattatacag aaaaatatct agggtcgctg gattctaagt aaaaaaaatt tttaaaataa
    55861 aaagaactaa atcaagtagt aattaaacta ctgttgattt agttctaata aaaaga
//
LOCUS       DARBEC010000005        52112 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.005, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000005 DARBEC010000000
VERSION     DARBEC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 52112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52112
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.005"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(71..1333)
                     /locus_tag="SCL80_000309"
     CDS             complement(71..1333)
                     /locus_tag="SCL80_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106205.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            complement(1326..2225)
                     /locus_tag="SCL80_000310"
     CDS             complement(1326..2225)
                     /locus_tag="SCL80_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106206.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="SCL80_000311"
     CDS             complement(2370..4148)
                     /gene="uvrC"
                     /locus_tag="SCL80_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEG1106207.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKKFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYRDQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELANKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGIDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIERLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="SCL80_000312"
     CDS             complement(4330..4644)
                     /gene="trxA"
                     /locus_tag="SCL80_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEG1106208.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            complement(4731..7091)
                     /locus_tag="SCL80_000313"
     CDS             complement(4731..7091)
                     /locus_tag="SCL80_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEG1106209.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEKQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLSDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            complement(7206..7753)
                     /locus_tag="SCL80_000314"
                     /pseudo
     CDS             complement(7206..7753)
                     /locus_tag="SCL80_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
     gene            complement(7811..8248)
                     /gene="zapA"
                     /locus_tag="SCL80_000315"
     CDS             complement(7811..8248)
                     /gene="zapA"
                     /locus_tag="SCL80_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEG1106210.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            8450..9367
                     /locus_tag="SCL80_000316"
     CDS             8450..9367
                     /locus_tag="SCL80_000316"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEG1106211.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            complement(9623..10534)
                     /locus_tag="SCL80_000317"
     CDS             complement(9623..10534)
                     /locus_tag="SCL80_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1106212.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(10937..11515)
                     /locus_tag="SCL80_000318"
     CDS             complement(10937..11515)
                     /locus_tag="SCL80_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1106213.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            complement(11535..12203)
                     /locus_tag="SCL80_000319"
     CDS             complement(11535..12203)
                     /locus_tag="SCL80_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106214.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEAKELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            complement(12218..13288)
                     /locus_tag="SCL80_000320"
     CDS             complement(12218..13288)
                     /locus_tag="SCL80_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106215.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAVGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            complement(13459..14823)
                     /locus_tag="SCL80_000321"
     CDS             complement(13459..14823)
                     /locus_tag="SCL80_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1106216.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQAIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEHTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            complement(14820..16379)
                     /locus_tag="SCL80_000322"
     CDS             complement(14820..16379)
                     /locus_tag="SCL80_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106217.1"
                     /translation="MRRKLLLLKSEMLGGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     ISVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQICYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFTITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            complement(16435..18915)
                     /locus_tag="SCL80_000323"
     CDS             complement(16435..18915)
                     /locus_tag="SCL80_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106218.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLADIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISTSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSVQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYEAKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWELTAGPTS"
     gene            complement(18899..19234)
                     /locus_tag="SCL80_000324"
     CDS             complement(18899..19234)
                     /locus_tag="SCL80_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106219.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPVPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEEKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            complement(19248..20282)
                     /locus_tag="SCL80_000325"
     CDS             complement(19248..20282)
                     /locus_tag="SCL80_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1106220.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTIPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNEFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            complement(20372..21052)
                     /locus_tag="SCL80_000326"
     CDS             complement(20372..21052)
                     /locus_tag="SCL80_000326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1106221.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGSLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            complement(21336..22439)
                     /locus_tag="SCL80_000327"
     CDS             complement(21336..22439)
                     /locus_tag="SCL80_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1106222.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHRLIIPHVLRDVELPDTTTHFDEEMLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDNGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            complement(23068..23538)
                     /locus_tag="SCL80_000328"
     CDS             complement(23068..23538)
                     /locus_tag="SCL80_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEG1106223.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLDSNQAIISELTV
                     GGLVDSLALAEGFTGEDIYEMFSEVRQLKKGEKCIEVHHESPNVRFKRGDNEMIFKNH
                     VSDHTELYEIVNQILSGIFELGPYEFKEENGNPKLNEEWQETIETQRNNPHLFQ"
     gene            23868..25508
                     /locus_tag="SCL80_000329"
     CDS             23868..25508
                     /locus_tag="SCL80_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEG1106224.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAAAILEKYEDQITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQIILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     EGLTPGQIPFARKRSDFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQNLNREEITDIKEAVESAKWVPEYKSLED"
     gene            25662..26984
                     /locus_tag="SCL80_000330"
     CDS             25662..26984
                     /locus_tag="SCL80_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEG1106225.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFWDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRTVKPSIPQLGVGLMVSL
                     TFFILGQIGNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(27052..27486)
                     /locus_tag="SCL80_000331"
     CDS             complement(27052..27486)
                     /locus_tag="SCL80_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106226.1"
                     /translation="MLREMRLEDAEALREINADQLGYDIPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKYAEKRGIGKALMTGVEEE
                     AKKRGITTIRLNSAEYRVEAHQFYEHIGYHSDKMQKRFLKIL"
     gene            complement(27551..28388)
                     /locus_tag="SCL80_000332"
                     /pseudo
     CDS             complement(27551..28388)
                     /locus_tag="SCL80_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
     gene            complement(28957..29262)
                     /locus_tag="SCL80_000333"
     CDS             complement(28957..29262)
                     /locus_tag="SCL80_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEG1106227.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(29287..29718)
                     /gene="ruvX"
                     /locus_tag="SCL80_000334"
     CDS             complement(29287..29718)
                     /gene="ruvX"
                     /locus_tag="SCL80_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEG1106228.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            complement(29715..29981)
                     /locus_tag="SCL80_000335"
     CDS             complement(29715..29981)
                     /locus_tag="SCL80_000335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEG1106229.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPAYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            complement(30236..30961)
                     /locus_tag="SCL80_000336"
     CDS             complement(30236..30961)
                     /locus_tag="SCL80_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1106230.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            complement(31322..31392)
                     /locus_tag="SCL80_000337"
     tRNA            complement(31322..31392)
                     /locus_tag="SCL80_000337"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31358..31360),aa:Gly,seq:ccc)
     gene            31509..32261
                     /locus_tag="SCL80_000338"
     CDS             31509..32261
                     /locus_tag="SCL80_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1106231.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            32277..32756
                     /locus_tag="SCL80_000339"
     CDS             32277..32756
                     /locus_tag="SCL80_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEG1106232.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(33007..34587)
                     /locus_tag="SCL80_000340"
     CDS             complement(33007..34587)
                     /locus_tag="SCL80_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEG1106233.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(34580..35323)
                     /locus_tag="SCL80_000341"
     CDS             complement(34580..35323)
                     /locus_tag="SCL80_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106234.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            complement(35499..36275)
                     /locus_tag="SCL80_000342"
     CDS             complement(35499..36275)
                     /locus_tag="SCL80_000342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1106235.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            complement(36523..37359)
                     /locus_tag="SCL80_000343"
     CDS             complement(36523..37359)
                     /locus_tag="SCL80_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEG1106236.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            complement(37370..38299)
                     /locus_tag="SCL80_000344"
     CDS             complement(37370..38299)
                     /locus_tag="SCL80_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEG1106237.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            38491..38844
                     /locus_tag="SCL80_000345"
     CDS             38491..38844
                     /locus_tag="SCL80_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1106238.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            complement(38918..39574)
                     /locus_tag="SCL80_000346"
     CDS             complement(38918..39574)
                     /locus_tag="SCL80_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1106239.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            39800..41734
                     /locus_tag="SCL80_000347"
     CDS             39800..41734
                     /locus_tag="SCL80_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1106240.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            complement(41820..43043)
                     /locus_tag="SCL80_000348"
     CDS             complement(41820..43043)
                     /locus_tag="SCL80_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1106241.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(43037..44935)
                     /locus_tag="SCL80_000349"
     CDS             complement(43037..44935)
                     /locus_tag="SCL80_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEG1106242.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            complement(44929..46047)
                     /locus_tag="SCL80_000350"
     CDS             complement(44929..46047)
                     /locus_tag="SCL80_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1106243.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            complement(46130..46963)
                     /locus_tag="SCL80_000351"
     CDS             complement(46130..46963)
                     /locus_tag="SCL80_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1106244.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            complement(46956..47873)
                     /locus_tag="SCL80_000352"
     CDS             complement(46956..47873)
                     /locus_tag="SCL80_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106245.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            complement(48100..49224)
                     /locus_tag="SCL80_000353"
     CDS             complement(48100..49224)
                     /locus_tag="SCL80_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1106246.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDSSDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            49407..50315
                     /locus_tag="SCL80_000354"
     CDS             49407..50315
                     /locus_tag="SCL80_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1106247.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            50456..52069
                     /locus_tag="SCL80_000355"
     CDS             50456..52069
                     /locus_tag="SCL80_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1106248.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
BASE COUNT        15575 a        10555 c         8890 g        17092 t
ORIGIN      
        1 gaaataggtc tatacaaaaa agaagctggg aacaataaat gtccagcttc cttctatatt
       61 tttctatatt ttatgctttt tttcgttcat ttctcataat tgaaatcgat tgtttcaatg
      121 aactccataa tccgccttcg ctatacacga gtacattcga tttgtacatg tttgcagaca
      181 agatcatcaa taagaatgtt acggctacaa gtaccaacaa ggaaatcaat gctccaccca
      241 taccgactgt ctcatttgct aagcgaaccg gcataatata tgaagataag aatggaatat
      301 acgaagtgat ccgaataacg atattgtttg gatctgaagc tcctagtatc aatccaagca
      361 tatatccgcc tagagataga taagtcactg gcaaaattgc ttttgctgta tcttctgctt
      421 tgttgaccaa agagccacat aaagctgcta aaacagaaaa aatcaaaatg ccaaagatca
      481 tgaatagaag agaataccac aagaatggtc caatcaagtt atccaatgag ataccgtcta
      541 atatattttt gaccatttcc atatctttga attgactgta tcctatacca aaaataatac
      601 cgtataatgc catttgcgtt aatgcaacaa gtaaaacacc tgttagtttt ccgtaaaaat
      661 gcttttgtgc agtcgtactg gacaggatca cttccatgat ccgcgtgcct ttttcagaag
      721 cgatctcttg tgcaatgatc tgcgcatacg tcaaaataat aatgaacaag acaatcgttg
      781 ctacataact tacgacatat tggactgtcg agttatcttc tccaactgtc atttttcctt
      841 ttgaatcaaa attgactttt tgtcgagtca aacttgcttg ttggctcaag ctagctactt
      901 cttctggtga aatgcctaat tgacttgctc gaagcatcga ttgcaagcca gtcagttgtt
      961 gttgtatcag tagttgtgta ctttgtccta atgaattttc gctataaagc atcccattta
     1021 tcttatcatt ctcagttgag actactaaat acgcatcgat tttttcttcc gaaagctctt
     1081 tttgtgcttt ttcttcagaa tcaactgctt tgaactcaaa atcatctgtt ttgacttggc
     1141 tcatttgctg actgatttgc tcattatcag ccaatacgcc gattttattg acatcactat
     1201 tttgttgagc aaagtttcct gctacataaa tgatacccag catgataaaa gggactaata
     1261 tcatgatcaa gaaagaaatc gacttaacat tttttaaata gacatctttg gtaataatcc
     1321 aaaacttacg catcgatttc acctgctttc aatttgaaaa tctcttccaa agttggcggt
     1381 tgctggttaa acataggaat gtagccgaac tgagtcgcac gagtaaagat ttctttccca
     1441 gcttcaggat cttctaaggt aatctctaac gtattgtctt catgaaggat cacttttttc
     1501 acgccttcga tttcttctac ttcctgttgg cttaaaccag attccaagaa taacttcgta
     1561 cggccaaatg attcacgaat ctcatgtact tttccattca aaaccatttt tccgtttcgc
     1621 aacatgatca aatgatcgca gattttttct acattatcca tattgtggct agaaaaaatg
     1681 acacatgaac catgtttttt caattccaat attccatctt ttagcaattc agcatttacc
     1741 ggatctaacc cgctgaatgg ctcatccaaa atgatcaatt taggttcatg gattaacgtg
     1801 gcgatcagct ggactttttg ctgatttcct ttagacaaag attttacttt atccgtcttt
     1861 ttccctttta cctgaaattt ttccatccaa aaatcgattt ttggttcaat ctcttttctt
     1921 gatttccctc taagttctgc aaaataaagc agttggtctt gaatcgttac tttcggataa
     1981 agtccccgtt cctccggaag ataaccgatg acatcataat ctttctcccc aagatgatgg
     2041 ccattccata acacttctcc ttggtcttgt gtcaaaaaat ccaagatcaa acgaaaagtc
     2101 gttgtcttcc cagccccgtt ttggccaatt agccccatga tttttccatc ggggatttgg
     2161 aaagagacat gatcgacagc tgtgtagttc ccaaaagttt taacaagatc cttgacttct
     2221 aacatttctt cactccttct tcaagaaatc acatatatcc ttagacacca gacgtaagcg
     2281 atttattatt taccaacaaa acgtaaacgc tctttttgtt gtacataaaa tgataggaca
     2341 ctttactttc tttgtaaagt gccctttcct cattcctgat gtaatttatc aaatacgttt
     2401 tgtgcgacat ttttcggcag tccgatttct tgtaattctt ctgtcgtggc tgctgtgata
     2461 tttttcaacg atttgaactc tttaagcagc attttttttc gttttggtcc taacctttca
     2521 atttcatcta atcgggaagc aaaactgttt ttactgcgta gctgccgatg gaaagtgatt
     2581 gcgaatcgat ggacttcatc ttggattcgc tggagtaaga aaaactctgg tgaatttcgc
     2641 tcaagcggta ctacagccaa atctataccg aatagcaact catttgtttt atgtttatcg
     2701 ttttttgcca tgccagcaat aggaatatcg attcctaatt gattttctaa gacatctttt
     2761 gccacatcta cttgtccttt tcctccatcg atcaagatca aatcaggtaa aggcaaccct
     2821 tcgcgaatca ctcgtgaata tctgcgatag atgacttctc tcattgatgc ataatcatcc
     2881 ggtccgttga ctgtttttat tttatatttc cgatattctt ttcttgccgg ttttccatca
     2941 atgaatacga ccatcgcagc cacaggattc gtccccatga tattagagtt atcaaaagac
     3001 tcgatacgta ttggggtcgg gatattcatc gcgtttccta aacgttcaac tgctccgatc
     3061 gtccgttcct gttttctggc aatcagatca aaccgttcat tcaaagcaac tgaggcattc
     3121 ttattcgcca attcaactaa ttttttcttt tctcctcgtt ttggttgcag cactttcgta
     3181 gctaacaaag cttctacact gggcttgtcg atcgtatcag gaatcaagac ttctttagga
     3241 atgaaatgct cattttcctg ataaaactga ccgaggtaag tcaagaaatc ttcttctgct
     3301 tcgttataga aaggaaacat tgaaacatct cgttcaatta gctttccttg tcttacgaaa
     3361 aacacttgga cacacatcca tcctttgtca atcgcataac caaatacatc tcgatccagt
     3421 aaatcagtat ttgtcatttt ttggcgagtc atgattgtct caatggcacg gatctggtct
     3481 cggtattctg cagctttttc aaactccatg ttttcagcag ctttttccat tttctgatgg
     3541 atctctgact cgattttttc gtgtcctccg tttaaaaact ttttcacttc atcaacgatt
     3601 ttcgtataga ctttcggatc tacgtcgaaa taataaggac acagacattg tcccaaatga
     3661 tagtaaagac aaggctcctt cgtctgacta ggtttgcatt ttctcagagg aaataatcga
     3721 tcgaggatct tctttgtctc attcgccgca ccgacatcgg gatatggtcc aaaatacagt
     3781 gctttgtctt ttagtacttt acgagtaatc accaaacggg gatatttttc attcgttatc
     3841 ttaaggaaag gataggtttt atcatccttt aacataatgt tgtatttagg atcatttttc
     3901 tttatcagat tgatctctaa gagcaatgct tcgatattcg attctgtaac gatgtattca
     3961 aagtcttcga tttcactaac aagccgttcc gttttcgtat tatggctgcc agtaaaatat
     4021 gagcggacac gattttttaa tattttggct ttgccgacat aaatgatcgt tccattttta
     4081 tctttcatca agtaacagcc gggctgatca ggaagtaaag ctaatttgtt tttgattcgt
     4141 tcgttcatct ggtactcccc ttcatattca attaaagtta ttatagcatg tctgtcaaaa
     4201 aaacaatcca gcctgacaac gtaattatac tcaaaaaata ttaatttgaa taaccttaac
     4261 tcaaccgcaa aaaaaccgag gacggaactg gattcagtcc caccctcagc ttatttttgc
     4321 tgtaagtttt tataaatatt gatcgatcat ttgacgtagt tgttcttttg aatgaacacc
     4381 aacagctttt tctactactt gaccatcttt tttcaacatt aatgttggaa tgctcatgat
     4441 accgaattgt tgaggagttt ctggattttc atccacatcc attttagcaa ttctgaattc
     4501 ttcttcatca tattcttgtt ccaactgatc caaaatcggt gcttgcatac gacatggacc
     4561 acaccaagtt gcccaaaaat caatcaatac aagtccttta tctgtttctt cattaaatgt
     4621 cttatctgta attacttgtg acatatcaaa gacctccatt tttaaaatct atgattagta
     4681 tagcatttct atacttggat agcgaaccat ctgcttacct ttagtaagct ttacttaaat
     4741 ttaacaattg tcgcaccatt tccgccttgg tttgccggtg caaactcaaa gcttttgacg
     4801 ctccgatggt ttttaagata atcagtaatt cccttgcgaa gtgcgcctgt tccttttccg
     4861 tggacaatag tcacttgagg ataaccagct agaatggcag aatccaaata ttgatctacc
     4921 tcacttagcg cttcttcgta tcgttttcct cgaaggtcca attgattcgg cacgtgacta
     4981 ctagattcag atcgtacagc atggataact cgttgtgtcg gctctttttg aggagcaaca
     5041 ggcgtcagct cgtcttcgga cacattcatt tttaatatac cgatttctac ttgccactga
     5101 ttcccgtttt tacgtaaaag cgtccctctt tggccgtaag tcgtaaccaa cacttcgtct
     5161 cccggtttca acgttttttg ttctttcgcc ttcttcaata ctttattttt cttcaaagtt
     5221 tcttcttgat gtagatttgc taattgggtc tttgcatcga tcaactgatg ctctttcaca
     5281 tttccttgcc caatctgctg ttgcatttta cgaatatcag caatgatttt ttctgctttt
     5341 tcttccgcct ctgacacgac ttcgtttgct tttttctttg ctttttccat ttctttttca
     5401 cgttcttcaa agaaatagct gtaagcttct ttcaaatcat cgtgaagttc ttgtgcctca
     5461 gaaacaaaat gacgcatttc cagatattcg gtctctgcca ttttccggcg attctctaaa
     5521 tctgtgatca tttcgtttaa atcttggctc tcatcattca tcaactgctt cgcttcatca
     5581 ataacttctg tatcaagacc taatcggcta gagatttcaa acgcattgct tcgtccagga
     5641 acgccaatca gcaaacgata cgttggactt agtgtatcca catcgaactc catgctggca
     5701 ttaatcgtat ttgcccgatt gtatccataa actttcaatt caggataatg cgttgttgcc
     5761 atcacataag cagacttttt accaagatca tctaagattg cgattgctag agcagcacct
     5821 tcttgcggat ctgttcctgc accgagctcg tcaaataata ccagactttt ttcattgact
     5881 tttgataaaa tatctacagt gttcgtcata tgagaagaaa aggtcgatag actttgctca
     5941 atcgactgtt cgtctcctat atccgcaaat acttcctcaa aaataccgat ttgactttct
     6001 tcacctgcag gaatcggtaa tcctgcttgc cccatcagtt gtaacagacc taaagtcttc
     6061 aacgtgatcg ttttcccacc tgtgttaggt cctgtaatga cgatggcttg atagtctttt
     6121 ccgattgtga tgtcattaga aactactttt tcctgatcga tcagtggatg tcttgcttgt
     6181 ttcaagacga catgattgtc ttcactgatt cccggcacga tggcttttac ttctttacca
     6241 aatcgtgctt ttgcattcat cagatccatt ttaccaatca cataagcatt gtggatgatt
     6301 tcttgacgat ggggaacaag ttcagcagaa agttcactta aaatacgttg aatctcattt
     6361 cgttcagcga tttggtattg acgcaaacga ttattcaaat ccaccacttg ttttggttca
     6421 ataaataaag tctgtcctga agcactttga tcgtggacta caccgccaaa tacaccgcgg
     6481 tattcttgtt tgactgggat aacgtagcgg tcatttcgca tggtgatgat cgcatcactt
     6541 aaatactttg catttttccc acggacgatc ccatctaact gttcacgaac agcttgttca
     6601 cttcgccgaa tgttctgtct tatgctcttt aactcaggag aagcgtcatc agtcacgcgt
     6661 ccgtcttcat cgattgcttc ctttaaacgg cggctcaaaa cagggatcgt gactaactga
     6721 tctgcccaca tatacaaacg tgcaaattcg atttcactgt cagaaagatc atcaatgaaa
     6781 cgattcaatt cgcttgtcgt agacaatact ctggaaactt gtgcaagctc cactccattc
     6841 aagtctgctc cgatctcgat ccgtttcata tggggacgga tattttcaag tttaggtata
     6901 gggatcccgc ctcgcagacg ttgcactttc aatccatcct cagtttcttg aagccagttt
     6961 gtgatcgttt gtttctcact cactggtacc aattctgcca gttcttcttt tccttgtgca
     7021 gtcactacaa attgtctgac catctgtttg actttttcaa attccaatgt ttcaaggatt
     7081 cggttattca ttttatcacc ttatctttca ctatgaaaaa agagctgtgc cggacatacg
     7141 ctacgcagcc ttcttaaata aactactctg tacaaaaaac gaaatatttt ccagcactat
     7201 tttccttaat tcaaaatatt cgttacccaa agctcataca tcttgttggt aagaaatggc
     7261 gttctctcaa cgataaaacg agcgaaacta tgctcgccga attgattttg gaccacacta
     7321 atggaacaaa ggcaagtaac tgcaaaatca aaaatatagt aacgtaagcc atgattagtg
     7381 acaggatgcc tcctgcaatc cagtctacct gtctcaagat cggcacatac gtcaatccat
     7441 ggacaaaaac ccccacaaat cgggtaagca gccagccaat tagcaaaata atcagaaaag
     7501 cagttcctgc ataaaaagct tcatctaggt gaaaagcaaa tacctgatca aaaaatgcta
     7561 actttgatgt tggggtgaca gctgggtatg gaatgtataa ttccaaatga cttgctaatg
     7621 gtttgtaaaa atgctgggca gcaataaatg acaatacata tccaatcgcg aatatgattt
     7681 gcaaagcaaa cccgcgacgt gctccagaaa aaaacgcaat tagcaataaa aataaaatca
     7741 gcaaactcag catgctacgt tcctttctct tctaactttt ttaaattttt cttattttga
     7801 taaaaggatc ttaattagaa gatttttgtt taatttgtcc ttttcgtttc tcgtttaaaa
     7861 tctgttgtgc ttccacatga tttttaatgg tatgatctgc ttgaccattt tctttcaata
     7921 cttcccgagc ttcgttctcg attgcttcaa tgcgtttcac acgattttca agctctgtaa
     7981 atttgatcat ttttttcttt aattctgctg tttcttcttc cagctccaag attctttctt
     8041 gtttcttcaa ttgatccgaa agcgcattga cagccatcaa gattgctgct tgttcattat
     8101 ccatttgtgg tgataattgt ttcaattctg ctaactgttc attgatcagc ttcgttacga
     8161 tatccatatg atgcttggtt tcccgtccga taatagtata tgtttggttg gcaatcactg
     8221 ctttgtagcg tgttttttcg tgcgccatac gaatcatttc ctcctatttg atgaaagttt
     8281 tatttagtgt accatgaagt tggttctttc accgttctcc acaaatattt ttcacatact
     8341 taactattat atgataaact ttaaaaaaat gctatgtctc gcaatctctt agaaaacgaa
     8401 aagtcgtgag ttgcggtata gttttatgta taaaaacgag gagaatttta tgagcaacgt
     8461 cattatcaaa gcaacaaacg accaacttag tcaaatgaaa gcatactatc gctcttattt
     8521 gttaaataaa cagattcctt atactagctt tgctgcaaaa aaagacggga cgaccattac
     8581 cgcttataca tccggaaaag tgatgtttca aggtacgaat gcagcaatag aagctgataa
     8641 atggggggag gcacaggata aacaagccaa gccagcaaaa acagcctctt ctttgccaaa
     8701 aaatatcagt caattatccg ttttaggtag tgatgaggtc ggaaatggca gctattttgg
     8761 tccgctaaca gtatgtgctg cttatgtgaa acgtgatcag cttgcagaat tacgtaaact
     8821 tggtgttaga gattcaaaag agttaaaaga tagccaaatc atccagcttg ctgacatctt
     8881 aaaaaaaacc atcccctata aactgcttgt cttatctcca gaaaaataca atgagatcca
     8941 accaaaatac aatgccgttc ggatgaaagt cgctttgcac aatcaagcga tccatttgct
     9001 gctaaaaaaa atcgagccag aaaaaccaga agcgattttg atcgatcagt ttacgccgga
     9061 agcaaatttt aaaaaatatg cgcgtttaga aaaaaatcaa ttacaagaaa aactgtactt
     9121 cgttactaaa ggagaacaat atcatttagc tgttgctgct gcttctatca tcagtcgtgc
     9181 cagctttttg gaagagttac acaaagcatc agctgaagct ggaatcaccc ttccatcagg
     9241 agctggaacg aaatctgacc aagtagcggc aaaactattg gagaaaggcg gcatgcctat
     9301 gcttgaaaaa tatgccaaac ttcattttgc caatacagaa aaagctttga agctgctaaa
     9361 aaaataatgg aactgtgaca aaagtcatgt cacagtccct tgttccgaat aaacggtgtt
     9421 ctcatgccaa ctcctcggtc acaagtcaca atagtcatca aacatgagaa gctgtttttt
     9481 gaaattgctc cttgtgacgc ggagttaaac ggcaggctca caacctcttc cttaaacggt
     9541 gttcaaaagc ttgcatctgc gataataagc ccggccaaac gaaaaatatg aggagctatt
     9601 tttgtttggc tgggcttatt tattactgaa aataagtggt caatgcttgt gtcaaacttg
     9661 cagcaatctg ctgctgataa ctgtctgtta ctaattcctt taaatcacta tcattattca
     9721 tataacccat ctcgattaaa atagaaggtt gaagattttc tcgaagaact tgatagttcc
     9781 cagtggagat tcctcttgaa tccagaggca gatcagtgaa ggagtcagca attgtctgtg
     9841 ctaatgtttc ctcttgtcca tagtaataat atgtcgtgat tccagttgct tcattcgcat
     9901 attccgtaga gtctgcgtgt aaactgataa agacatctgc ctttttttct tcactgtagt
     9961 cgcatatatc tccaagctgt actaattcat ctgtagtatg ggtcaaataa accgttgctc
    10021 cagcatcctc tagcgccttt tttactttta tggctgtact taatgttata tctttttctt
    10081 ctgtttgttc atcattgctt aatgccccag tatcatttcc gccatgccca ggatctaata
    10141 caatcactgc atcagatagc tcttccggtt ttgtttgtat attctttgtg actttggttc
    10201 catcttcgag ggctaaatat ccagtatagc catcatttgt cttgatctga taagcatttt
    10261 ttgtttcaga aagaaactcc gccagctctc cgccgtttat agttgcaagt ggggtagtta
    10321 gcgagtcatc tgtatatagt gtacggtcat tttttccgat tttgattaat gtctcttgtt
    10381 ctgtatcatc aaatgattcc tttgtctttg taaccgtact gttcgtactg ctgaccgttt
    10441 gtccaactat ctttttttga ccaaaaacat ctatttgaaa acagataata atgataatga
    10501 ttgcaaatac aaaagctcca attatttttt tcattcttga ctctcacctc gtagatccag
    10561 actaagggtc aaaacttaaa atcagcttaa gtacgcaaaa aaggggctgt aaaagatata
    10621 accgtagaaa ataacattac tgctaaaatg atttttttca tttttggcat atcctgaaat
    10681 ctccttttga ctattcgtgg tgattcatct acccaataaa tagtttcctt cattctacaa
    10741 acaattaatt tcttcgttat taaattaaaa cacttcctgg aacaccgaca taataaataa
    10801 aaatataccc tgataatcat gagagaataa caaatttgta tttatgaaaa actacctata
    10861 taaaaaaaac aagatataac gatcttcgtc atatcttgct tcttcaccta actatttttc
    10921 tccctatata acagctttac gcttctccta atacaccatt caaatataaa tcgatcaatt
    10981 gcgccacatt tagcggttct tccacaggtc ttttcgaccg agtgatgtaa ggtgctaaca
    11041 ctaagaaaaa atgccttgct gcgatttctt ttgaaatccc atttcttagc acagattggt
    11101 ttgctgaaaa gatttcttca aaaggttcac gataattttt cttccataga aagaaaagat
    11161 gatctcttgt ttcctttgaa agattgcttt caagatcgtg aagcatcaga aacaaatcga
    11221 ccagatgtgt ctcaatcaaa aactgcgcca tttttgtcaa ggttacttga aatcccgttt
    11281 cgcttgtttg aacgatttct ctcaaagctt ttccaacatc ttttaagtgg ttttctaaaa
    11341 ctgccgtata tagtttttct ttatcaccga aataatgata gagattgggt tgggtgatcc
    11401 caactttttg tgcgatctct cttgttgaag tgccgagata tcccttggac ataaacaagt
    11461 cagtcgcttc tttcaaaatt ttttgctgtg tatctttgtt gtatcgatgg accatttttc
    11521 cacctgcttt ttggttattc tttttctttc aatacaccat cttccatcat aaatattcta
    11581 tcgcatttgt caatcagccg tgtatcgtga gtaaccatga tcgttgcttt atttttgtct
    11641 tttgattctt ttgccaagat atcgactact tctcgtgcct tttctgaatc caaactagca
    11701 gttggttcat ctgctagaat aatggacgga tcattgtaca atgcacgagc gattgcgacc
    11761 ctttgccgtt ctccaccaga tagttcttct ggatacttgt tgatcaactt atccacgcca
    11821 agctgctcaa ataattcttt tgcttctttt tgacgatgtt catgtttgat cttatctact
    11881 aataataact gcttttttac tgtcagaaaa ggaaccagat tggaagcttg caaaataaag
    11941 ccaatttctt taaaacgtag tttaacgcgt tcactttctt tttgattact gaattcttga
    12001 tcattgataa atacagagcc actgctaggc gtctgcaatc cgcctgccaa agtcaaaaaa
    12061 gtacttttac ctgaaccaga aggaccgatg attgcaacaa attccccttt atcgatactc
    12121 atggttgttt ctttcaatgc tttaactact tggtcgccat cttggaaact tttttctact
    12181 tctttaaatt ctattgcttt catgctcttc ctccttctta tccaattgcc ttcaacggat
    12241 cgatttttac gattgtccga acagaaaata gtgcgcctaa cacagctact accagcatca
    12301 acaagctaat gacgccaaaa aacagccaat tgctttggaa tggaaccgca tcaggtagaa
    12361 caagggatgt tcccactgtt cctaatagac caagaagaat accaacaaat gataaaatga
    12421 atgtttgtgc cactacagaa cgcgcaataa atcctcctgt gatcccttgt gctttcatta
    12481 caccaaagat attaatcttt tgcatcgtca gtacataaat gaaaatcccg atcacaatag
    12541 ccgcaataac gatcaaaaat ccgatcataa atccaaaagt caatacttgc gcattatatc
    12601 caggtaattc attgatgaat ttagaaattt tgatctgttc taaatcttca ggtaaattac
    12661 tgattttacc tctgacaact attgcgttaa tccgtgcatt ttcacttgta tcttcttttt
    12721 cgaagcggat ctcttggtaa gcattgatgg ttgtatagag aactggtgag acattgaatt
    12781 tggcatgatc cgtaaagcca gttagtttaa acgtcttgtc actaccagat aacttaactg
    12841 tatctcctac ggctagacca tattcttctt tcaaactaat atcgcctact gcctcatcat
    12901 cgttatcaaa agcttttcct tcaacaagat tgggcatgat gaattgattt ttatcgatac
    12961 cgaagaagct aacattgatt tttccttctt ctgtactgtc tttactagtc acgactccag
    13021 gtgtctgggc aagatacgcc actttcccac cttcgacttc ttctgctatc ttcttagtaa
    13081 tcatggacat ccctagattt gaatttgcat ctttcgaaag cacaatcgaa tctgcctccc
    13141 acttgtcgac tgctgttcga ttatcttgag ctaagccata agctaaccca gtcaaaaaaa
    13201 ataccaggta ggcgatcaaa aacatcaccc ctgcaactaa cgcataacgt aactttgaat
    13261 gcataatttc atttaatgct aaaaacattt aactcctcct cctgaaataa tcattttata
    13321 caacaatttt atcacttgat aaatttttgg cgaatattat gctcaaaaaa ttcttccgct
    13381 atttcattat atgaattttt tattctcgat tgattaatat ttattatatc cccacacaat
    13441 aaaagaccgt gatatttctc acagtctttt atctactcta ttgctatttg ctctcttagc
    13501 tagtacttta aaagctaact cattgaggta gtgaaaacga gaggtgtgtt cagacagaaa
    13561 tccaaaatcc tcaactgcta gctgtggaat cgtagttttg tgtttcacga tatccgcaag
    13621 caggttcaaa tctgataata agaattcttg gcttatcagc tgtccgccaa ttatttctaa
    13681 tgtatccgga tggtagatca gctttgcatt tactgttttg ttttcttcat cgtattgtgg
    13741 aaatagatca aattctcctg aacacgaacc ggtattttgt tccaagaatg gcgcttcatc
    13801 ttctgtcagc cctacagttc ctaaaaagta gtctataatg actgctgctg ttatcttttg
    13861 tctgaggtcg taagcggttg gctgaccact caaagttagc gctactgctc ttgccatatg
    13921 tacagctcga gtaactaaag gtaaaaagag atgccgttta tttttgtcaa aactgactgg
    13981 gacgatatct ccaatcgcat agatgtccgg atcacttgtc tgcagatatt cattgactat
    14041 cacagtctca tcctcataca aagccacttt ttcttttaac aatgtcgtat taggacgagt
    14101 ttgggcagaa aaaatgacca tatctgcctt tattgcttga gatagaagtt gtacggatac
    14161 tactttctct tctgattttt tccaatcgat caagaattca tttggataaa aattgatttg
    14221 cttagtttgc agctgatttt gaaaactgtt gatcatatcc ttatcgaaat atcttgataa
    14281 aacattcggc atataatcaa ctagatgaat aatgtatcct ttatttctca aagcatctgc
    14341 taattccaat ccaatataac cagcaccgaa gatcactact tcttttgctt cttttaaagc
    14401 ggcaagtact tgcttagatt gcactagatt tttataggtg tagacatttt cagcttcaat
    14461 ttgtttatac aaggtgacag gagaggaacc tgttgctacg attaatttat cataggttat
    14521 ggtctcgttt tttccttctt tggatgcgag gatgagctga tgtttgtctg gatcaagttc
    14581 agttactctc cattcggtga tcagttttgc cccactttct tgaagagtcc gcatgttatg
    14641 ggcaacttca gctaaactac gtatctttcc ttttaaataa aggttgattc catttgaaac
    14701 atagcctagt ccatctttat gatcttcatc gatcagtaaa acttctgctt tcggttgaag
    14761 ttttcttaga tttagggcag ctgtgatgcc tgcatgggaa gcaccaatga tcacgatttt
    14821 catgttttat tcatccttaa attcaatttt tctaaataac attcttttaa aaattggttc
    14881 aaatagggga aatcataatt atattcgttg ctagtgaata caaaaacttt agcggacttc
    14941 tgtgacgggt actctttcaa ttggctagtc aaaatgatat catactgctt ttctttctcg
    15001 tattcgacta aatcatattt gatcgtattc agcaattgtt cctggatgat ttctttcacg
    15061 atatccgcca ttagcctttc ataaggaaga tcataagcaa tttgcagacg taattctaac
    15121 tgcttgatca gcgtccgcaa aatagatgca tagcgagcaa ataattcaaa gcgggcattt
    15181 tcctcgtcta cttcagtaaa atcaacaata gcaaatgttt gtctgaccaa tctttcacat
    15241 aaaaccagga attgtttatc ttctacttca gttaaccgct gttttaatcc ttcttggcca
    15301 aaaatattca tccatcctct aaaataggca aaatgagcgt gattttgcat cactgtcaac
    15361 gcaatctttt gcgaaatctc ttctggaaaa acataaaaag agaaatattc ttctatcgat
    15421 tgtttaaacg ttcgattcaa ttgagctact acgtcgtctt tgtatttttc gcttatcaaa
    15481 aactcctgga catgcgagtt atgtgtatct aatgtaaagt tactgatcat gaaaagataa
    15541 aaaaccaacg tttcattttg attccaagga aaggcatatt gaaagaagta acgggtcaaa
    15601 aatgtatgga ggtccaataa aaattgtgtc gtttgaaaca atccaacagg aagattgcta
    15661 taatctggtt tggatttttt cgtacagcgc attcgcttct tcataatgga aagccatatt
    15721 tcaaatttca atcgtccttc ttctgtaata gtaaaactaa actctttttg cagcagatct
    15781 aaaaaagtgt ttcctgataa atcactgtat tcttttttgt tttcaagata aggacgtcca
    15841 aaccaaaata attggaaata aaaataacaa atttgacttt ctggaccggt cagccgcccg
    15901 ctttttattt tcagatgaaa ctcatttaaa gaatcattta attccttcac tctgcgagac
    15961 aaagtagccg aactgatatt aagttccatg acaagtttct gccgatcaaa tgagccattt
    16021 tccaacagat aaagaagtat ctgatatttt gtagaatagc gcaagtagca atgataataa
    16081 acatcgactg ctgctatatt tccatgtctg attaataaaa tattcccttc ttggataaca
    16141 atttccaatt gtttgatatt ttccaagtta gcttgtaaat aaaacagatc ttctttcaag
    16201 gtagcccgat caactgataa gcgggtaagt acgttattaa cggaaatctc tcctttttcc
    16261 tctaataaaa gttggaaaag tttgatttgc cgttgttcat ttttttcaag caaactgaaa
    16321 agttccaagt ccactcctcc aagcatttct gattttaaca gcagaagttt ccttctcatc
    16381 cttgaaaaag aggatctgtg acagttatca cagatccata caaattctaa tcacttagct
    16441 ggttggccca gctgtcagtt cccatgtgac catgccttga tacgttgcgc caggttgagc
    16501 attcgtactg ctatttacct ctaagagtaa accatcggta tcgggaaagt caacgagaac
    16561 ctctccttta gctgttccat cactctcata aatcaacgtg ttttgacctt gctgaatcaa
    16621 ttgttttgat ccagaatgat tgaagtataa ttttgttccg cttaattctt gaccttgatc
    16681 attcttaaat aaagaagtca atgtccctgt cagttgccaa ggagacgtgc ttggaagccg
    16741 agtatcccgc acatcaaaag aaaaatcgcc ttttgcttca taataacttg tcttcaacgg
    16801 tttggggaga tcgttaccaa atgtaaaatc atctggtaca ttaactaatg ttaattcttg
    16861 tttggggaca agaattgttt tagcagatgt ttggacaact gggttaggaa gcttagcacc
    16921 atctgctaat gtaatattgc tggaaagtac agctgccttt gtcagtatgc tgacggattg
    16981 tacagaagta tctacagtat ttaattgata gttgtaagta agatgaatgg tatctcctat
    17041 tttgctcaat agatctgctg ggacagaaac tccttgttga atgccggtat ctgcgatagt
    17101 tgtaccattt aattttaagg aaccattgat ataagtagta ttttcaggaa gtgagatcgt
    17161 gatttttttg tcggttatcg catccccaga ttttgttaac gtgaatgtca cattttcttg
    17221 aatcgtatct cctgatagag cagacagggg atctcctgtc tcaggttgtc tgctaaggtt
    17281 ttttagctga ctatctgcac tcacagatgg aaaattagcc actgttattt gaggaagagt
    17341 ggtgattcca gcttgttcag actgccattt gtacgcaaaa gacggataca tgttagtaag
    17401 tacatcgtcc accaagcgat aatcaaaata atattgccct gaagccaatg caggtagtgt
    17461 agaagtagct gtatttacac ccccgttatt tgattggatc gtttggaaaa gttgatctgt
    17521 ccatcctgtc gctaattttt gggttccttt cgaataaagc ctgtaatgga tcgaaccctt
    17581 ttttagttca gttgctgacc aattggccgt gacttggtac gatgcagaag aaaggagctt
    17641 tccccactga ctatcggctg ttaagtttgt tggtaaaacc gttccgtcta gcttgtactg
    17701 ctccaatacc tttaaagcaa caggagaaag caatccgcct tgcgcatctt tcccactgta
    17761 cacgatattg gctgtctgat tcaatccact tgtgcttgct ggagtatgag tcagtattgc
    17821 tccttgtgta acgttttttc ctgtaaaata gttgactacg cgatcaaagc gactaaatgg
    17881 caactgatca aaacgtcctt cagtcagccc catcgtcaat gtccgactat tggtagagcc
    17941 tggaaggttc tggttatttc catccacgta ttctgtggta tcagcaaatg tattatattt
    18001 tcctgttgaa tcacttgttg agatgtaact attccacgta tttatctctg cacttgcaga
    18061 aaatgcatat gctcctggat tcaagtttcc gccagttaat ttaggataaa atctacctgc
    18121 agctggagct aaaatcgaat tgaacatacg atcttggata atattattcg aatcataaaa
    18181 atcaacagaa aaaacggcct ttttagacat agaaggttgt aatttaccct tggtcaaaat
    18241 tttgtttgga ctcgtatcat atttatctcc gttttctgtc atcttcgcga cattagctgt
    18301 aacatcaaat tgaagcggga tatcgtccac gttgaattcg cctcgcgtaa taatagcaat
    18361 aatatctgcc aatagagtat ttgtatccca cgaccccatc ccaaaacgca aggtatggct
    18421 tcctgctgat aataaactac cgctagtaac cttgatgggg gcaccggcaa tatacaagta
    18481 ggcgttgttc cagtcaattg cttttaatac atcactgctt gatacatgtt ccggcaaatt
    18541 gatgatcgcc acaaatgcaa atccttgtgc agtgatattc accaaatcaa gtacatttgt
    18601 tagtttcaca tggctttccc aatgaaaacg gaccgtgcca cctttccaag gatcatttga
    18661 accatcgttc aaataagtag cagtcaattt aggcaattgg ttagccccag ttagttggac
    18721 aagcttcccg ccattgactg cactacgtcc accatttgga ggtacgatat tttcatacgt
    18781 gtatggatca taagaaatgg caggaggcgt tgctccaaca gcttctgccg aaggtgaaaa
    18841 gataaatata cttaaaacaa aaagcgaagt aaaaaagaag gagcgaaaaa atgaaaattt
    18901 attttgtagc aacatgttca tcctcctttt cctttcttct ccataacaac aaccacgcaa
    18961 gaagccataa ttctatacca atgatcaaat aaagaaaacg gatcttttct tcattcgtct
    19021 ccgggaatac gccatgttga ttagattctt tataaatcgg tagctgtgtc tgctgttttg
    19081 atgcgccatt gactgaacca cttggatgcg gaactggact tagatcataa aatgtaatcg
    19141 tacctgaact tttggtatct gcttggactg gctcttgcca gctgaagaac agcaaacttg
    19201 caataatccc tcccaataag atcgatgttt tcaacgaaat cacctcatca tgttgtttct
    19261 ccttttttct ttttcttgtg gacccacaaa aacagcaaaa tcaaccataa cactagaact
    19321 gcagcaacca atcctgcata aagccatggg aaagattgtt cttgtactgc aaaaacggaa
    19381 ttttcattta ctttttgtgc tttttctttt ttgatcgtaa agttttgtgt aaatcgccat
    19441 tcattactgt cgcctgattt tgctatgatt tctgcgcggt aatcgccggc ttttgcttta
    19501 tcaccagcta aatcaagatt caagtgaaaa ttcgaatttg gtgcaactaa aaattcatta
    19561 gacgtatttt caatataaag ctgattttca taaaagatct tgctcgtaaa tatcaaattg
    19621 ctgataatcc taggctgagg attttggaaa cgaagctgaa cagttggctt tgcgttgacc
    19681 tcgtccaaat ctcctgataa aagtttcaga tccggttcta tcgtctcttg actttcttgt
    19741 aaaacgactg cgagattata agcaatacga tttttgattt gtgctccttc tgtagcattt
    19801 tctgtttcag cttcttcttt aggtgaaaca tgaatacccg ctaatacacg tcccttaaag
    19861 ctttttgctg ggattttcac ttctataggt atagttgttt ctgcatgtgc tgatacattg
    19921 atttcctttt tcgcaatgag caaaacgtca cggacatcaa acggcaatgt ctcatctaac
    19981 gttgggtttt tttgactata atcgatcgtt ccaccatcac tcgttaccgc cggattgaca
    20041 gagacctcaa atgttcgttc ttcatctgaa gaattgctga taattacttc tagtgtctga
    20101 ttttgttccg gtgccactaa taaatcaaaa taaccgattg ctttatctat ttggttctct
    20161 ggaaataccg gtttgattcc aaagtctcct gctgaatcct ttgcttggct gattgacgga
    20221 aaaatgaaaa atagacttaa aatagaaaaa aacgaaacaa acagtcgttt actttgattc
    20281 attgtccacc acatcctttc aacaacaact gcggtaaaaa atccttcacc tatagtattt
    20341 tcaccgcagt ttgttgaatt tatttcatac tctatgctac tgggccagcc acgaggttcc
    20401 aagtcaaatc agccgtgaat gttccagctg ctgctttcgg cactttcagt gtgatatttg
    20461 ctggatcata aacagaaagc catgttccca gaccttgatc ttttgctgct gtaaatattg
    20521 gtgtcgctgc acttgcttta ctcaacccag ttacagtttg actagttggt ccagtggatt
    20581 cgttatctga cgtactttta acagtggatg ttccaaaatc taattcagcg ccttgcaaaa
    20641 cttgtgaacc gtttacaaaa tctgaaaccg aaactgtcac gttccaccct tgtgcttggc
    20701 catctgcacc tacacctcga cgatctgata cttggagata aggtgtatcg ttttttgaag
    20761 cgtctacttg atacgttttt gttccgcttt caatctcgtg tgtaccaaat ggcaattcag
    20821 gaactacatc caatgttaaa gaacctgtct ggccagttgc tggattttcc ggatctgctg
    20881 gctctactgg ttttgaagga tcagtagggt taagaggctc aacggtattt gttggttcag
    20941 taaaggtaat atctcctttt gaagtcgctt gaccctcagc aaaagctggt gttgccaagc
    21001 cgcctagaag aagtgttgaa agtaataatg tcccaatcat gttctttttc ataataaatc
    21061 actccttttt cttagtgacc taagtatagt agagacagta tgcaaaaaat cctcatgaat
    21121 tcatccgctt acttgaaatt atgacccttt ttgtataaaa gagaaaaaaa caataaaatc
    21181 gtttacatga atattcttcc ttttttatta agtatgaaaa aatatttcaa ttttattttt
    21241 tcgtttcaaa ttattctatt agtgacatcc ttgaactata aaaataggag tacggcatca
    21301 tctgccgcac tcctattttt ttcgtcattt atttcttaaa taaaacgatt ctcacgcaat
    21361 tttatttttt tgatatcttc tactgtttgt gttccagcta attgcatgac catttccaat
    21421 tctgttttaa agaaatcaaa cacttgatgg actcctgtac ttcctcctaa agacaatcca
    21481 taaataacgg gtctgccaat agcaacaaga tcggcacctg atgcgattgc tttgaaaaca
    21541 tgttgtccac gtcgtacacc gctgtcaaac acgattggta cacgtttatc tactgcttct
    21601 gccacatatt gcaacgaatc aaacgcagca ggccccccat ctaattgacg gccaccatgg
    21661 ttagataccc aaattccacc agcaccagat tctaatgatc gatagacatc ttcttctgat
    21721 tgaacacctt tcacgtagac tggcaaatca gaatgagcag cgataaattc tacatctttt
    21781 ggactaagtt tttgtttgga agatttatat acagcatcca tcgtttggcc tactcctgat
    21841 tgataagcct gaacgatcgg cattggcagc gggaaggtaa acccgtttct gcggtctgtt
    21901 tcacggtttc cgccaactgt tgcatccgct gtcaatacga ttgcttttgc tccattacgc
    21961 ttggccactt caagaatatc caaattgatt ccattatctt tgctcatata aaattgaaac
    22021 cattgcggtg ctttctcccc gcctgcttct cttatctctt ctaaagtaca agaagcgtaa
    22081 gaacttgctg tatagatcgt cccaaaatct gctacacctt ttgcagaagc tttctccgcc
    22141 ttcacatgag ctaatccatg agcagcaact ggtgccatga tgattggtgc agtcagcatt
    22201 tcttcgtcaa aatgtgtagt tgtatcgggt aattctacat ctcttaatac atgaggaata
    22261 atcaaacgat gattgaatgc ccgttcattt tcttgataag taaataagtc gccagcacca
    22321 ctgctgatgt aaccataacc accagctgga atcacttgtg aagccgcgat ttctaaatct
    22381 tccatattga taaaatctac tattccttca tttgtacctg cttgatatgt tttttccatc
    22441 tcaatgcctt ctctctataa aatttgaatg atgaacaata tgatagcgtt tacacttttt
    22501 atattactct tcgacattga tacaggcaaa taatctgttt acatttcagc cattccagat
    22561 gtcactactc tggaagaaat gattgtaata aaaatagata aaaaaccgat gggactttga
    22621 caaaagtagt gtcaaagtcc ctttttttga ataaacggtg ttcaaaagcc attttctcgg
    22681 tattaagtca aatttatgca aaaatgtgga aagccatttt cacaaaattt ttcttactac
    22741 tcgaaactaa gcgacttttt cacaacctct cgataaaacg gtgttcaatc agctgaccct
    22801 cggtattagc tcaaaaaatg gcaaaatatg aggaactatt ttgccatttg tctctctaat
    22861 actcaggtca gaacgttgac ttcacagtct cttccttaaa cggtgttcaa aaacttgcat
    22921 ctgcggtaat aagcccaacc aaacgaaaaa taggaggagc tatttttgtt tggctgttct
    22981 tattacttga tgcagggcag gtttttcacg acctctgatt cacggtgttt ctttttcatc
    23041 aacattttta cgaaatactg tggaagacta ttgaaataag tgtggattgt ttctttgtgt
    23101 ttcaatcgtt tcttgccatt cttcatttaa ttttggatta ccgttttctt ccttgaactc
    23161 ataaggtcct aattcaaaaa tcccagacag tatttgattc acgatttcat atagttcagt
    23221 gtgatcagac acatgattct tgaagatcat ctcattatca ccacgtttga acctcacatt
    23281 cgggctctca tgatgaacct ctatgcattt ttctcctttt tttaactgac ggacttctga
    23341 aaacatctca taaatatctt ctccagtaaa accttcagca agcgccagac tgtccacaag
    23401 accgcctact gtcagttcag agattattgc ttgattggaa tctaatttta atgtatagct
    23461 tgtcgcttct actacgccac atgcattgca accgccttct gatagttcgc tttttctaat
    23521 caattgaact tctttcattt tctcttcctc ctcttaattt tctacttatg tatttttatc
    23581 acctgacata ggtgactcct atcaacgcta tcatgtgctt ttttcctcag aaatcctatt
    23641 tatccattcc atctatccct tattcttcta cttttctttc tctagcatat aagagatacg
    23701 catagtattc aagcaaggat tgtcgatata aaaagaacaa atactgctat tttgatttct
    23761 tttggctata ttacatataa ataaaattta tcatacctgc tctttttgac tatatcatca
    23821 tctgacttct atacttcgtt tgtattaaaa cttaggagga atcattcatg cttacagggt
    23881 taaacttatt aaataatcct tttctaaata aaggaacagc ttttacaaaa gaagaacggg
    23941 caaaatatgg aatcactggt atgcttccaa gtactgtcca aacactggaa caacaatctg
    24001 ttcaagctta tggtcaatac ttaagcaaac aaacagattt ggaaaaacgt atcttcttaa
    24061 tgaatctatt caatacgaat cggacattgt tttataaatt gatgggcgaa catttagttg
    24121 aattcatgcc tgtcgtttat gatcctgtcg ttgcggattc aatcgaacaa tacaacgaaa
    24181 ttttcttgaa accgcaagat gctgcatttc tttcaatcga cgaaccagaa aacatcaaag
    24241 caagtttgaa gaacgcggca gatggacgag acattcgttt gattgttgtg actgatgcag
    24301 aaggaatctt aggtatgggc gactggggtg tgaacggtgt tgacatcgct atcggcaaat
    24361 tgatggtcta tactgctgct gctggtatta atccagcaca agttttgcct gtatcgatcg
    24421 acgctggtac taataatgaa gaattattga ataatccctt atacttaggc aatcgtcatg
    24481 ctcgtgtaga aggcgaaact tactatgaat tcatcgatca attcgttcaa tcagctaccg
    24541 aattattccc agaattgtta ttgcattggg aagattttgg acgaggaaat gctgcagcga
    24601 tcttagaaaa atacgaagat caaatcacta ctttcaacga cgatatccaa ggtacaggaa
    24661 ttgttgtcct agcaggtgta ttaggcggat tgaatatatc cggtgaacaa ttgaaagatc
    24721 aaataatttt gacatttggt gctggtactg ctggtgtggg tatcgcaaac atccttttag
    24781 atgagatgat ccgtcaaggt gtacctgaag aagaagcacg ccaacatttc tatcaagtag
    24841 ataaacaagg tttattgttt gaagatacag aaggattgac tcctggtcaa atcccattcg
    24901 ctcgcaaacg ctcagatttc actaatgctg atgaattgac aaacttagaa gcagttgtaa
    24961 aagcgatcca tccgacaatc atgattggta cttctaccca acctggagca ttcactgaag
    25021 aaatcgtgaa agaaatggct gctcatacac cacgtccgat catcatgcct ttgtctaatc
    25081 caacaaaatt ggcagaagca aaagcaaaag atttaataga atggacggac ggaaaagcat
    25141 tagttggaac aggtatccct gctgctgatg ttgaatataa tggtgtaact taccaaatcg
    25201 gtcaagcaaa taatgctttg atgtacccag gtttaggact tggtttgatt gcttcaacag
    25261 ctacacgagt aaatgctgaa atcatttctc aagctagtcg agcattaggc ggaatcgttg
    25321 atgtaacaaa accaggagca gctatcttgc ctccagtagc aaaaatcact gaattttctc
    25381 aaacgattgc tgaaactgtc gcaaaatcag ttgtcgcaca aaacttgaat cgtgaagaaa
    25441 tcacggatat caaagaagca gttgaatctg ctaaatgggt tcctgaatat aaatctttag
    25501 aagattaaga tcgttatagt aaaagaacat acagaaactt tgtatgacgt ctaaaaagaa
    25561 aaaagcgact gcggcaaaac agcggccagc caactcctgc tcttttgtcg cttttctttg
    25621 gcttttttca tatgatacag aataagaaag gatctgtaaa aatggcacaa aaaacaacaa
    25681 ctgcaactgc agttgaggaa aagaaaaaag aaggtttttg gtccacaaaa atcaccggcg
    25741 taagtttgcc actttatttg atcatggtcc ttgtcctgat cattgcaatg gctttaggga
    25801 aattaccgac gaatatgatt ggaccaatcg cagttttggt tattttcggt aatttatttc
    25861 attttatcgg gacaaaaatc cctatcgtca aaagttattt gggtggtggt tccgtttttg
    25921 cgatcttcgc ttctgctgcg attgctacat ttgggatctt acctgaatat gtagtgaaat
    25981 ccacagaaaa ctttgtcagc aatatgggct tttgggactt ttatattgct gccttgatta
    26041 ctggtagcat ccttggtatg aaccgtaatc tattgatgaa agcttctgtt cgttttattc
    26101 ccgttgcact tatttcaatg gtcgtctcct tttttgccgt aggactcgtg ggcatgctta
    26161 tcggaaatgg ttttgcaaat tccgttttat acatctcatt acctgcgatg gctggtggtg
    26221 ttggagccgg agccgttcct ttatcaacga tttatgcaaa tgttttaggt acggatgctt
    26281 cttctatcat ttcccgcctg attcctgcga cagcaatgac aaatgtctta gctatcatcg
    26341 gtgcagcatt agttgctcgt gctggacaat caatgccaaa attcaacggc aatggtaaat
    26401 taatggaaaa aggcgatctt ggcgatggaa aacctcgaac tgtgaaacct tccattccac
    26461 agttaggtgt tggtttgatg gtatcattga ctttcttcat cttaggacaa attggtaact
    26521 attttgttcc taaagtccat gcttacgcat ttatgattat tatcgttgtc atctgtaaaa
    26581 tcgccaacct attaccagag tattatgaag atgcagctat catgttcaat aacttgattg
    26641 tcaaaaactt gacagcagct gttcttgccg gtatcggtat tgcgttgctg aatctgaatg
    26701 tattagcttc tgctttgaca tggcaatttg ttgtcttatg tctgacaagt gttatcgcga
    26761 tttcagttgt ttcaggattt gtcggtcgtt tgtttggttt atatccaatt gaatcaacta
    26821 ttactgctgg cttatgcaac aactcgatgg gcggaacagg aaatgtagcc gtcttatctg
    26881 cagctaatcg gatggaactt attgcttttg ctcaaatggg caatcgactt ggtggtgcca
    26941 tcgttttgat catctctggt ttcttgatgc aattactttc ttagtcaaaa aaacaaaaat
    27001 acctgatcat caagtatcgg cttggtgatc gggtattttt ttgataaaga tttacaggat
    27061 tttcaaaaat cttttttgca ttttatcgga atgataaccg atgtgttcat aaaattggtg
    27121 tgcttctact ctgtattctg cagaattaag ccttattgtc gttattcctc gttttttcgc
    27181 ttcttcttct acgccagtca tcaaagcctt tccgatgcct cttttttcag catatttaga
    27241 cacagcaagt gccataatat tgaacattgg atcgctaaaa atcgattcat aggactccgc
    27301 ttgtacatag ccagctgctt ttttagaagt aggatcttca taaaccaaga aaaaattttt
    27361 ctctggctcc ttcagcagtt tcttcatttg tctcaccgta acggttaatg gtatatcata
    27421 ccctaattga tccgcattga tttcacgtag tgcttctgca tcttctaaac gcatttcccg
    27481 taacatccaa atcaccagcc aatttctgat tttttaaaat gaattttttt atcatagtat
    27541 actctttttt tcatttagaa caatatgttt cagtgatttt gacttgcgtt agtctagatt
    27601 ctcctaatgt ttgtaagatg acaaaggcat tgtaacaatt gaccaaaaga accacgagga
    27661 caattacttg aaaaataaag gtcacttttt ttggagacaa aaccctgcta aatcttgctc
    27721 cccacaaacc tccaataaca gcagcgggga taacataaaa caacatagat aaatcaaagc
    27781 gcataaagct acttgttaaa gcaatcgtta ccaattttgc cagttgagaa aagaaaatcg
    27841 tagatagaga atatagtgtt gcttctttta ttggcaatgc aaacatcagc atcaacaaag
    27901 aaacattgac tggtccacca ccaataccta aaaaactagc taaaaagccc agaacccccc
    27961 ccacatataa ggtaccaagt ccatgacgtc aaatgaaatt ttggctgatg atgcttggtg
    28021 tagaagaacg caaaaaccaa ggtcaatacc gtcaaaaaaa tttggatcag ctgcacatgc
    28081 ttctcgtttt caaataactg taaaaaaact tcgaatacaa tgttgccgac tactcctcca
    28141 acaacagctc cactagatac ccaaccgaca atctgccaat tcagtgattt cccgcttgat
    28201 acttgtcgca cagtcgaaac aatggacatc gtgaataccg caactgttga gtaaaaagaa
    28261 atccctgcaa cagaatgggc gccgataaga tcaagaatcg gtttgatcaa cacgcctcca
    28321 cccatcccgg aaatagcgcc aactgtgttc gccagaacga tcaccacaaa ataaatcaga
    28381 ccaatcatct tgtatttcct ttctcttgtt tatatctttt agcgattgtt actcttttct
    28441 tttattatac agtaaaagaa acaaacgagg cttcgaaagc ttttctactt tttaaggaaa
    28501 tttagtgcta aacgaaaaga ggttgtgaaa aagctgtcct gcatcaagta ataagaacag
    28561 ccaaacaaaa atagctcctc ataattttcg tttggttgaa cttattaccg cagatgcaag
    28621 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg ttcagctccg agtcacaagg
    28681 aacaatttca aaaaacagct tctcatgttt gttgaatatt gtgacttgtg accgaggagt
    28741 tggcaggaga acaccgttta aggaagagac tgtgaagtca acgttttgac ctgattatta
    28801 gagaaacaaa tggcaaaata gttcttcata tttcgccgtt tttcgagcga atgccgaggg
    28861 gcagctgatt gaacatcgtt tatttggaaa aagggattgt gacaagacgt ttgtcacaat
    28921 cccttttttt atatcttatt aattcattct tttgttctat tcttcttctt ccgccatgaa
    28981 tgtattgaat acttcttcaa tcatgtccca ttctgcgtct gtttcgattt gttctaactc
    29041 gccttctgtt ccatcttcgt tttcgatgta tgcataagct tgtaattcta catcttcatc
    29101 ttctgaaaca ccagcaggat ataaaagaac atagttacgt ccaaattctt cttgtccgtc
    29161 aactgttagt aggatttcat ataatgtttc atttccttgg tcatccacca atgtgatgtg
    29221 ttcgtgtcct tcatggtcgt gggaatggtt atgttctgtc ataagattac ctcttttctt
    29281 ttattattaa aataattgct tacctgagcc gtctaaataa ttttgtaaga tcatcacagc
    29341 tgctaattta tcgatgactt tttttcgttt ggctcttgaa gtatttgcct gctctactag
    29401 catacgttct gcctgaacag tggttaatcg ttcatcttga taatcaactg gcaaggagaa
    29461 tagttctttg atcttttctc cgtatgacat ggaagcttct gcccttggtc cgatggaatt
    29521 gttcatattt ttaggtaatc caactacaaa ccggtctact tcatattgtg ctactaattc
    29581 cttcaaccga tcaaagccaa actcttcttc tgcttcattg atccgaatga tctctactcc
    29641 ttgagctgtc cagccaaagg ggtcactgat agcaacacct actgtcttag aaccgacatc
    29701 caagcccatt actctcataa attcactcca tgatttgaga ggtagtcctt gacaattact
    29761 tccatgatct catcgcgttc gtgacggcga atcaaatttc gagcatcacg ataacgagga
    29821 atgtaggccg ggtctccaga aagcaagtag ccgacaatct gattgattgg attgtagcct
    29881 ttttcttcca aagcccgata aacaatcgct aatgtttcgc tcacttcttt tttgcgacta
    29941 tcgtcaaaac caaaacgtac ggtttcatct gtaaaaccca tgattctaca cctctttccc
    30001 gcaaggtaat tccagcttac cttctacaac cttattttac tcgaagaaag ggaaaactac
    30061 aaacaaatat tccaaacttc cgaataaatt aagaaacact atttgtatgt ttttttcctt
    30121 ttttctgtag ctttatcaaa agtactcttt tcataaagta tacgctatat ttctttgaat
    30181 gtatgttttt acacaatttt ttcataatat tcataaaaaa ttcgaatagc tgctactaat
    30241 tttttgtttt tgttgcttgt cctgtctcat aattgatcgt gtagccatct tgaacattgt
    30301 agataaagac attgaattca atttttttgt cttcaatgct ttctgcttcc aattgcactc
    30361 ccctggctac cagttcattc ccctcaaaat gaggagtgac acggtagcgt acatgatgat
    30421 ttgtttcttt gatatatgcc gcaatatcat tttcgtgagc aagcatgtat ggagcattca
    30481 acgaacgtgt ccctgtcatc aagtttttaa tgttgttatt ttgaccagtc agctggaaac
    30541 caattaaatg gctgcgatta tataaccatt gcccgtcact taatttcttt tgtttccaac
    30601 ctgtcgggtc aatatacaaa ggctctctct tttcagtagg gaacaaatct tttccaagca
    30661 tagcattggc ttgtcctact ctgttcaatt tatcaatatt tgaatagctt tcccatgttc
    30721 ctttggaaag gctaagatcc tcttttgtaa attccggctg attcccattg atttctattt
    30781 cttgatgttt tccatcatat tctggcacat ccgctacttg agaagcttgt ggtgtggaat
    30841 caaaaatagt tgtaatctgc tgaaagatat ctgaatcttt ttgtgtggtg gaaacaccga
    30901 atgcccctgc aatcacagca gcgacgatcc ccacgatcgc cttggttgtt tttttgctca
    30961 tttctttact cctttactaa cagctcccta aatcttaaca tatgttcccc tataaataaa
    31021 tactagaaag aaatagaaac agcaaaaaat aaaacctgtt ttaagatggc ttaaaaatac
    31081 aaaaaagaga cggattcctc gtctctaatc agtcaccact ctatctgtaa ggtatttatt
    31141 attattcaaa aattttcttc atactcaaga caatacactg atttcacaac cgcttggtaa
    31201 acggtgcgca aaagcttgca tctgcggtaa taagtccatc caagttaaaa acataagaag
    31261 ttgtttttgc ttggctgttc ttattacttg ttgcagagca gctttttcac aacctatttt
    31321 aagcgggtga cgggaatcga acccgcgaca tcagcttggg aagctgggat tttaccacta
    31381 aactacaccc gcaatacaaa aacagtatac gcctctttta ttccttcctc aagttttttt
    31441 attctttacg cttctggtat gttttgtagt aaaataaaac taatcaatca attattggag
    31501 aagatgctat gacgggaaaa gaattagatc tgttgctgga cacttgttta ctcgctggga
    31561 aaatcatgat ggaaagcaat gcggagatgt atcgggtaga agatacgatg agtcgaattg
    31621 ctttagcttc tggaaatttc cgtttggtca gctatgtcac tcaaaccggt ctctttattg
    31681 gtcttgatcg tacctcgacc atccgaatgg aacaaattac caaccgttcg attaatttag
    31741 aaaaagtagt aaaagtaaat gatctttcac gtaaatatgt tgcttcagag ctaacattag
    31801 aagaacttta ctgcgaactg caagcaatcg accgagaccg ttctttcttt cctgtctggt
    31861 tgcaaatcgt tagcgcggca gtgatcagtg gaacaattat ggtgttattt ggcggaaaga
    31921 tgaccgattt acctgtgact ttagtcattg gaggattggg atacgcagct tatctatata
    31981 gtttgaaatt ttttagaatc aaatttttat cggaattttt atcttcttta ttgattggca
    32041 tagccgctat ctacagcgta cgttttggct taggaagcaa tcaggatcta atcattatcg
    32101 gatgtgtgat gcctttagtt cctggagttc aaattacgaa cgctatccgt gatttgctag
    32161 ctggacatta tgtctcagga gtttctcgcg gaacagaagc gatgatgact gctacgatga
    32221 tcggttttgc gattgctttt attttccagc ttttttatta ggaaaggaac acaagtatgg
    32281 ggacgatatt tattcagttt tcttttagtt ttttagcgac tgcggcattt gccgttatta
    32341 caaatgtacc taggcgttca ttgatttgtt gtggtctttc tggtgctttt ggctggatga
    32401 tctactggat ctgtgttcaa ataggcggat ctgctgcttt tggttcattg cttggttctt
    32461 taggagtagc attcatcagc gatcttttct ctaaacgttt gaaaatgccc gtaacgattt
    32521 tcaatattcc cgggatggtt cctctagtcc ctggtggttt agcttatcaa gcggttcgaa
    32581 atctcgttac tgaatcctat cgagaagcgg ctcattatgc ggtccaagca attatgattg
    32641 ctggagcaat cgctttaggt cttgtattat cagaagtctt taaccataac atccgtaatt
    32701 tcaaagtaaa gcgtgacgca atcatgatga aacgaaaaaa acggcttttt aaataaacga
    32761 agtgtaacaa agaaaaggtt gtgaaagaga cgttcagcct tgagcgatga caaaatatct
    32821 cacaaacatt gcgacaagta accgcaggag caataagaag taattatgag tattaaaaaa
    32881 agttttgtaa aaatagcttc tcacattttt acataaaatt gacttaatac tgagaaactg
    32941 acttttgaac accctctatc tgtaaaaaag gggctgcgac atgatttttg tcacggcccc
    33001 tcttctttat tcaaattttt tccaaaacgt tcgttgataa taaacgatcc atagagcgga
    33061 aagtattaga actaaaccga tcacaggaat attcactaac caaaatggat aaaacaaagc
    33121 caacattccg tataagacga taagaataat actgatgatc aatgaaccca tcatagttgc
    33181 gactaaaccc aaacttcctg aacctctgtt aaataattgg ttaatattgg tccagttcag
    33241 cagcagcaac cgataatctc tagcgaaata acgtagtgac aataagtagc agccgaataa
    33301 agaaccaaat agcaagctag ggataaaaaa caaaggcagc tgaaaagaca gtccgcttaa
    33361 taatgcgatc ccaccagtta aaaaactctg caatatccat ccgaatctga acttttcttt
    33421 taaatattgg ttcatagaga ttggcaaaga acggataaac agaaaatttt cttgatccaa
    33481 agaaataaga ttgctgataa aagaagtctg attaactgtt aagaaagcca atgcgatccc
    33541 taccaggaaa acaacaccga ttaacttatt atctaaatca gttaatttta tttcgcctcc
    33601 tagggcaaaa gcaagaataa aaatgattgg agttaacaag gaagaagaaa gaacttgcat
    33661 aatcaaatta ggttctttta ctaattgagc attgtaacta aataaaagct gatgcacatt
    33721 ttggttcttt ttatgggttc tttttatttt gaactcactg tttgaatcag tcagttgctc
    33781 tgtaagcttt ggtactatca gctgttttac agcaaggaaa aatcccagag ccgtgatagc
    33841 tagtattccc caacccaata aaccttgttg agaaagtggc tgactggcag catcatagaa
    33901 tggcaataat atcgaaatcg gatgccgatc tgttaactca ttttcaatag tccccgtttg
    33961 atgattcatc cacatgattc cagcaaatgc gatcagcatc gagctgccaa gcaagatact
    34021 agaaactgtt tttctatgtt ttttaaacac tgctgttcga gccaaaccaa aaacaatcaa
    34081 actagccaga caaaaaagaa taatcaaaaa tagagaaaac aaaaggattg agataatgat
    34141 agtaagcaaa atccctagat cagctcgcca tccggttaaa ataaacatga ctagaagcgg
    34201 aaaaacaaaa ggaatgatgg tcaaagcaac taccaaaatc tttgctgaaa aaacctgttc
    34261 ttgtccaaaa ggcagaggca aaaatacttg caggtcattt ccttcaaaaa agatattata
    34321 aatgactgat atactttgag ttaaacctaa cataccaaaa agtgccacgt aataagtaaa
    34381 gaaacctggc aatttaacaa agttcatagc aaacatagca aatccgtaaa tcagtaaaaa
    34441 aatcagccct gaaaaaagat tttgagacaa aagatagata gttaatcgct ttcccttttt
    34501 tcctttctct cttgctttat tcgtgagctg tggattaaca tataaaagat taactcgaat
    34561 taattctaat aattgtcgct tatacatacg gtccatctcc tgtcgcttgc cttccagcca
    34621 ttttcaaata aatcttttct agcgtttcat caggtgagcc attcaataat tcactaactg
    34681 ccccctgata aatcaattca cctttcttca aaatagccag tttatcgcat aactgttgtg
    34741 cggtatccaa tacatgtgta gaaaaaatga ctgtttttcc ttttgcggca tgagccttca
    34801 tcatttcttt caaatcaaat gctgcttgag gatcaagtcc ttgcaaaggt tcatccaata
    34861 cccaaatatc tggatctggc agcaacgtgc cgatcaaaat cgttttttga cgcattccat
    34921 gtgaaaaact tgcgattgtt tcattttgat gactatacat atcaaaaagc gctgtcaatt
    34981 ctgctaaacg cttttctttt ttttgtgtgt ctaattcata ggcagctgaa atcaagtccc
    35041 aatattctcc agccgtcaat tgtaaaaaaa tatctgggga atcaggcaca taaccgattt
    35101 tttgtttgat actcaatcga ttttccgtca atttcattcc atctactaaa atcgttcccg
    35161 atgatggttc aataatacta actaaacttt taatcgtagt tgattttccg gcaccattat
    35221 gtcctaaaaa tccaaaaatt tccccttgtc ttatcgagag agacaagtct tttaatgctt
    35281 gttttttccc gtaactttta cttacattga tgaattcaat catttctgta ctccttttgt
    35341 ttgttattac tttttattgt acccttcgtt attggtggag taaatgtcaa aaagagtgca
    35401 taacaaaaaa ggatagaaac gactaggctg ctacttgggt tgttctcttc tacaggaaaa
    35461 cactcttcta acaggtaaga tatgagtctt tgttttcttt aaaatgattc attttctatc
    35521 ttttcttgca taaaattctt tagtttatca atacttctct ttcccatctc tagttgttct
    35581 ccatttgcaa atgttactat ttcgtttact ctgctaatag atgtaatgtt ttcagtatta
    35641 atgatataag cttttaaatc tttacataaa tactcatatt cgagtgattt tttatatttt
    35701 ttaattgtgc cgtttacgag aatctcttca tctttcaata ctaggcctat tttattttta
    35761 taacctggca tgctcattag ataaaggatt tcatcgtaat ggatatagtg atcgctatta
    35821 ccataacgga cagttgtcca cctttttttc gtagcatgtt gaacaggata gttttctttt
    35881 tctttcaata agtaactttg gatgcttttt ttattttttt cataagtgaa ttcttttatc
    35941 aaataaccac taggcgtaat ctttcgatta ataacagtta ttgctttatt cggatgagac
    36001 gttagataaa caattttaca cttttcattc ttttctctaa gcatttcgcc tagttcgata
    36061 ccgctataat aggcgttcaa atcgatgtct aggaaaaaaa tatctgagga atagatttca
    36121 ttatttttaa tgaattcaac aaattgtata acgttcaatc cctgaacgat ctcaaatttc
    36181 caaggactta ataaaattct ggattcttcc aaaatttcta ctagcatttt ttcaaaaacc
    36241 aaactatcat ccaaaataaa tatacgcgtt accataaaaa gtacctccac tcgatatttt
    36301 actatcaaaa tcatttcata caacttttca acatacatgt taggaataaa ttgtaattct
    36361 cttcacaatc ttttccaaca cctttgaaac cgatatcaaa tactttcagg atgctgcttc
    36421 taaaataaat atcaaacaaa tgaatacatc atgcaaaaaa agccagacaa ccgacgtcct
    36481 tgcgtgtcag ctgtctgagc tatgtttatt tattttgttc ttttaaaaag aacgatacac
    36541 atactgattt ttcggagcag agactttctc agcgtttgta acaactacag atgggatttc
    36601 acgcttgaat ggttgataat agtctgattg gatggttcct tctattttat accattcatc
    36661 atttttaacc gttactcctt ccggcatatg cgtcaacaaa ccaaagactc ctgaatctgc
    36721 cacacaatgg ataatgccga atcggaacaa gaagatatct gtattgctat cgttcggtgt
    36781 ttggtaaaca aatccttcat agctgatttt tttgccaatg aattcgctag ggtaattata
    36841 aattagctcc ataacttcta aatagttttc atctgtgatc ttgatagttt gcttatcccc
    36901 atacttttct ttcaactcat tcatttggtc taagtagtct gatttgttga aataaatact
    36961 ggtatccggc tttaagtatt gtgtctgcat ctccggatcc cctactgatt ctttacttac
    37021 tgggaaatta aatcctttgg cttctacgat cgtagtgtcg agactaactg ttggaaacaa
    37081 cgtccccacg attactggca aagctagcag tacataagcg attccacgtt gataaggttt
    37141 tgacaaccca tggtcatgat cgtgctcgtg tgccttttct tctgcttttc cgtttttgac
    37201 ccacaaatac agctgaacta aagccaaaag agcagataaa atcattgaaa gaatagctaa
    37261 gtagcgataa tggacattga tatattgatc gagtttgcca gaaacttgca agtacatcat
    37321 caattcgaaa tacccagata aaatcaaaaa ccgcagcata ttttccctcc taaataaata
    37381 atgtgaatcc aataacgcta agggcaatca gtccgatcag cgataaaata aagcgtcctg
    37441 taaaatatcg tttcatcatc agtagatttt tgatatcgac cattgggcca aagaccaaaa
    37501 aggcagctac aggtgctgtg ccgaacagac ttaacaaaga agatcctata aacgcatctg
    37561 cttctgagca aagagacatc gtaaaggcca ataacagcat gatgataatg gctaaaatct
    37621 ttgttgagcc gagcgttagc ataattctag ttggtaaata agtctgcatc gcagctgcga
    37681 ttaatccgcc aatgatcaaa taacgacctg tatcaaaaaa ttcgtcgatt gaatgtgtca
    37741 acacatgccc agtttgatgg atcagctgat tttttggttc attcttctca ctttgttctt
    37801 cttcatggac atgatgatgg tgattttctg ctactttaga cgtgattttt tctttcaata
    37861 cagcgtcttt ttgaaaatag gcaagccaga caccaatcac aagggcaaca aaaaagctgc
    37921 ctatcacccg ccagcttgcc atctgccaag aattgccaaa tgcgataaaa gtggaaaaaa
    37981 tcacgattgg attgatgatc ggagcagtca gcataaaagc aaacgctgtg tgaactggta
    38041 cgcctttttt aacaaattga tgaacgatcg gtacgatgcc gcattcacat gaaggaaaga
    38101 aaaatcctaa gatcgaacca gtaaaaatag agaagatacg attttcagga agccatctct
    38161 tcacacgttc tggggtcaaa aatacttgta aggcgcctga gataagacag cctaataata
    38221 cgaaaggtaa tgcttcaata acgatcgata agaaaatcgt actcatctgc aaaaccgaat
    38281 ttggaataaa agaaaacatg agtttcctcc tttttatttc tagttcattc gctttgcaaa
    38341 tatacgcttt tattcaaaaa aaacaagttg ctctgtaaat ttttacacaa ctcttatttt
    38401 ttaccaaagc acttcaattg actttttctg ttattcccat taaattaagg tctcatacat
    38461 atcacttcta tcataaaagg gagaataaaa atgaaacaaa ccactaactt gactgaagtt
    38521 caagacatac tgcaacagtc atccgttgcc gtcatttatc tttcgatgcc taattgcagt
    38581 gtttgtcacg ctgttcgtcc acgactagag gaacttctca cacattatga tattccggca
    38641 cttcatttgg atgcatttga aacaccagaa gtagctagta aatttgaagt gctgactgcg
    38701 ccagttgtcc tgatttttca tcaaggaaaa gaagtctttc ggcaagcacg gttcatcgac
    38761 tttaaaaaaa tccgttatct actagatgaa ctgacagcag aagatgattc cctttcttat
    38821 gaagaaatct tccgaacaaa ataatcgttg tccaaacaga aaaaagcgat tgaacaagtg
    38881 aaagttttgc cttcatttgt tcaatcgctt tttattttta ttctgctgta ttttcggaat
    38941 gcgcatttgc gtcagaatgc aagaaataga tcaaggtttg caattcattc gtcaaatcaa
    39001 cattttggat caatacatct gacggagcat tcaatcgagc agcggtaaag ttcaagattc
    39061 ctttgattcc tgcttccgtt aactgattga ctacttcttg tgctctttca gcgggtatcg
    39121 ttaaaattgc tacttcgatt tgttggatct tgatttgttc tgacatatct tccattggat
    39181 aaacggggat accgtctacg atgcgcccaa cgatttcctc attcacatca aaagcacaac
    39241 tgatacgtat gctattactt tgatggaatt tgtattttag taaagcactt cccaagttcc
    39301 ctacaccgat cagcgcaaca tttgtcaatt gatcgtcgtt taatgtcttt gcaaaaaagt
    39361 gcataagatc ttctacatca taaccatacc cacgttttcc taattcgcca aaataagaaa
    39421 aatctcgacg gatcgttgca ctatctactt gaacggcttc acttaattca gttgaagaca
    39481 cttttttctt tccagcatta tttaaaatcc ttaaatagcg ataatacaaa ggcagtcttt
    39541 tagctgtcgc ctttggaata atatgatctt tcacgaaatg acctccacgt attgtctaat
    39601 tacatctttg atttctccat catagcagta tttataggaa aaaatcaatt ccattgctca
    39661 gatattgtga aattttattc tttattcatt tatttatcat ttctgcaaat ctcgcagtat
    39721 aatggaattt atggtagact actaattgaa aaatagtttt ttatttgagg agaattcaca
    39781 aagtagaaaa gaggattcca tgatattatt acaagccaat cagattgctc gccattttgg
    39841 tgcagaagtc ttatttgaaa acatacattt agaaatcagt acaggcgctc gtgtcgcttt
    39901 agttggcaga aatggcgctg gaaaatcgac tttattaaag atcattgctg gaattgaagc
    39961 acctgatgaa ggaaagatcg ctaaaaataa aacagcgact ctcggatacc tcgctcagga
    40021 cacaggactt gattccaatg aaacagtttg gaacgagatg ctgaaagctt ttgaacaagt
    40081 tagacaaatg gaagagcgga tgcgcgagct agagattttc attagcgaag gtacgccaga
    40141 cacacctgct tatagttctt atttaaaaga atacgaccgg ctgcaacacg atttttcaga
    40201 tcgaaacggc tatggttatg aaaatgaaat tcgttccgta ttgcatgggt ttaaattcga
    40261 ttcttcattt tatgatcaga aaatcgatac tttatcaggc ggacagcgca cacgtttagc
    40321 tttagctcgc atgcttttgc aacaccctga cattttgatt ctagatgaac caacgaacca
    40381 tttagatatc gagacacttt cctggcttga gaattatctg caaagctata ctggtgcatt
    40441 attgattgtt tcccatgacc gttatttttt agataaagta gtcaatgaag tctatgaatt
    40501 aagcagaaga aaaatgcgcc attacaaagg taactattcg aaatatctag atttgaaagc
    40561 agaacagtta gcaagtgaat ggaaggctta tgaaaagcag caaacagaaa tccataaatt
    40621 agaagacttc gttgctcgga accttgttcg cgcctctacc acaaaacgag cgcaaagccg
    40681 cagaaagaca ttagaaaaaa tggaccggct ggatcgtcct caaggggatg aaaaacaagc
    40741 aaactttcta ttcagtatag aaaagccttc tggaaatgta gtcttgcaaa tcgaagatgc
    40801 cgctatcgga tatgataatg tcaccttatc tgaaccaatc aatctagata tccgtcgtca
    40861 agaagcgatt gctctagtag gtccgaatgg tatcgggaaa tcgacgttgc tgaaatcaat
    40921 cattcaccag atccctttta tccgtggaaa agaaacgcta gggacaaatg tcaccatcgg
    40981 ctattacgat caaggtcaag ctgatctgca tacaaacaag acgatcctag cagaattgtg
    41041 ggatgagcat cctaccactc cagaaaaaga gatccgaagt gctttaggtg ctttcttatt
    41101 ttctggcgaa gatgtagaaa aaccaatccc tctgctaagt ggcggtgaaa aagcacgggt
    41161 tgctttagcc aagttagcga tgaacaaaga aaattttttg attttggatg aaccaacaaa
    41221 ccatttagat attgacaaca aagaagtatt agaaaatgca ttgatcgatt atgacggcac
    41281 actgcttttt gtttcccatg accgatattt catcaatcgg atcgcgacaa aagtcgtcga
    41341 actctctgaa aatggcagca aagtctatct aggagactat gattattatt tagagaaaaa
    41401 gcaggaagaa gaagaaatcg ccgaattgtt ggcacaagat gagccagaat ctgctcctgc
    41461 tcccacgaac aaatctgatt attaccaaag caaagaacag caaaagctta tccgtagcct
    41521 tcaacgaaaa attactcagg ttgaagaaga aatggcgcgg atcgatgaat ggatcgatca
    41581 gttggaacaa gaaatggtcg cgccagaaaa cctggaagat cacgtaaaac tcaatgagct
    41641 gaaccaagat ttagaagctg ctagacaaga tcaagaaaca aaattgacgg aatgggaaga
    41701 actaagcttg gaattagaag aatatgaaaa ttaacgaaaa aaccaaaaca gaaaccattc
    41761 atgctgtttt ggtttttctt tttttatttt atcaaacaaa aattccatat ttaccttttt
    41821 caccaataag aagaccaatt actagtcatt ctcactaaag cttctaaata atagtaatca
    41881 ccccaaatac aaggtgtatt taccccatta tttttgggct tattataaac agactgtgtt
    41941 aaaaagccat cttcttcttc cgttgtagca tatctttctt tcaattgtcg cataattttc
    42001 caaacagcag actggtataa gttcttttct gttttgtcta atagacctga ttctattaat
    42061 tctaataatc cacaaaccat gatcaccata ctagatgtat ctttttcttc tctgctagaa
    42121 tcatcaaaga tcaaatccca ataacaaatg gtatcttctg gcaaccgatt cataaaataa
    42181 ttggctaata ttttgcttaa atcaaaatat ttttgttctt tcaaataacg ataacttaat
    42241 gcaaaaccgt aaatccccca tgcttggcct ctagcccaac aggaattatc actataacct
    42301 tgatgcgtac ttccatatct tggttttcca tctttgacat ccatataaaa tgtatgataa
    42361 gttgaaccat cttctcttac taaataatct gctgcatttt gaatatgagc ctgagcagct
    42421 tttttatatt tttgatttcc agtttcttca ctcgcccaat acaataatgg aagattcatg
    42481 caacaatcca tgatcattct accttgttgt gaaggatcac taggatctcc ccaagcttgt
    42541 ataactcctg ccttctccca aaaacgctga agtaatgttt ctgcagcatc aattgaagct
    42601 tccttcattt ttcgatttcc cgttatttta taatctgcta cggttgataa agaatacaaa
    42661 aaaccaagat cgtgatgatt agtgataatt tgacagtcaa tcctgtggat aaaatctata
    42721 tctgcttttt cagctgtttg ttgaaataaa ggatcacccg tcttctcaaa taacaaccaa
    42781 ttaattcctg tccaaaatcc ttctgtccaa ccaatattgt ctcttaatgg ataacgtcct
    42841 tttataggac actcgcttgg gaattgatac tgactgttac tgatacgttt gattaacctc
    42901 tccgtgtgtc tgcgacaacg ctcgatctca ttattccatt ctttttccat taattcttta
    42961 taacattcat ttatccattt ttctggatgt attatctctt caaacatcac tttatcaagc
    43021 atttccccac cagcctttag cataatttta gtcttttttt attgttttta ttttggatag
    43081 ttagcccggt tgtcccatca aaatcaattt tgggacgatt gttccatact tctaaactat
    43141 aatcccgatc ttgatgagca accactcctg ttccgagcca atgggttcct tttccaaatg
    43201 taccctctaa tccaggaata aaagtgtttt cccatgtcat caagtttgta ttgggcaaac
    43261 ccataatagc tgtggatttt cgtttaatag tatcgtcact taaacagata atacctgaaa
    43321 agccagcttc actcacccta tagttttcct tgtcttcttc tatgattatt ccttctttct
    43381 caggagaaca ttcaatagca aaccctgtct catatagaac atattctgta tctaattcta
    43441 ctttgtgtac gcgaatctgc cagccttcaa aaggaataag ccaagttctg atttttgcct
    43501 gtaaagtagg ttcccatgta gaggcaagta cctctttctt acatacaatg tctcgtacat
    43561 tatttcgaac gatgaactgt ccataggttt tgatgctctc attcttaaaa ctggtcgtga
    43621 tatatgtgtc cgctcgctga atccctagca ttgaatccat tgccatttct gctttcaatt
    43681 gattcgctcg cggtatcgaa aaaccaaatt gactagaata agcaaatttt aaatattttt
    43741 cattggtcaa tgtatggaaa tttggcccta attgtcccgc attcaaaaaa aatgtatgtc
    43801 cattatcatg ttgtatgagc aaattggctt ctgaaagaag tttagttcct tcacgaatag
    43861 gcataggcat ttctttagcc gtccaatact catggtcttc aggtagcgcc aacaataaaa
    43921 aaatcttatt cagccataat ggagataacg tactattata gggttcagtc aatgtcaatt
    43981 gagggtaggt ataaccaata ttcaggatac ctttttcatc aaatatttct ttttgcatcc
    44041 accatcttat attccgattg actaacccct tgatttcacc taatttaaaa ggcaaaagat
    44101 cggcccacaa catagctgac caaaagcttg aaactgcaaa acgataaatc atgcttcgac
    44161 caaaaggcac atttgcacca tcttccgcaa aaaaatgaat atagtctcta gaaaaatcaa
    44221 tagctcggtt cttatatatc tggctaatgg gcttttctgg ttctagtacg ctataaatca
    44281 aaccataata atgaaatgca aaaggattat aataatcttg gcgacctctt gaactgtctc
    44341 gataccagcc attttgaaga taacaatttt ctatattatc aaatgcttct ttcagtgctg
    44401 attctttaac tggataacca aatttttgga taattgttcc gacaattatc ttaaaaaatt
    44461 gccaattacc atctgcacat tcatattcaa aaatttgcga gaaccaatga ataatctggc
    44521 ttttttctcc tgtatctaat tttttccata ataattgttc atgatgatat aacgctaata
    44581 acattgcggg catttctacc atacgctggt ctctttcatg tatttctccc caataatcgg
    44641 gatgggaagg atctgttcca gcttttattc ctgatagaat taatttcaac catcgttcat
    44701 ctaattggtc aattgcgggc cccgctcccc ataaaacccg agagaatcct tccattcctg
    44761 caattttatt tccatatcct accccttgat gtatataatc aatttgtgcg ccacttgctc
    44821 gataaaacgg ctggagtggt tgatttatct ttttaaataa gtcaaagtaa tcctgtctgg
    44881 tccttaatag tggtactgga tgtttcattg tattttctcc tctttttact accaaatagc
    44941 taattccgac tgttctaatc gaagtaagcc ttcaataaaa aaataatcag aatagatcag
    45001 tggcacatta taagtcagtt ctctatggta tgcttctgct ccgcctccaa tgatcccatc
    45061 ttcatccata ttccaatttg catagtgctt ttctgtcttt tggagtatat tgatagcagc
    45121 ttcatgatac atttttgatc gtacagagga aacatatcct gctaattcca gaagaccgca
    45181 tgcagcgcat actccagcac tggtatcaat attcgaatct tttacagcaa aatcaatgag
    45241 aggaatataa ccagtttttt cgattttctg aataaaattt actgctatct tttctgctat
    45301 agctagatag tacggatttt ttgtgtaacg ataacttaac gcgaatccat ataaagccca
    45361 tgcatttcct ctgctccaac aagaattctc attgtatcct tgcccgccaa gtaaccgaat
    45421 atatttccct gattctgcat caaattctgc gatatgcccg gttgatccat ctttcctcac
    45481 tagatactta gctgttgtat ctgcatgctg ccgagcaatt ttagagaacc tcggatcacc
    45541 agtttccctc gaagcccaat acagtaatgg taaattcatc atactatcaa taatcatcca
    45601 accaggttta tggtcattcc atgctactaa atagttccct gaaatattat agcgtccagc
    45661 aagtaccgta gctgcatgta accctagttg tcgactatgt ttgttttgag actgttgata
    45721 atgtgcaata gccgatggta agaataaaaa acctacatca tgatgaacgt ctgaaaaatg
    45781 gtacagggca gattctaata attgttcttg ttgtattgct gcctctttga acttttctcg
    45841 ttttgtcttg tgaaagagct gccataaaat accactccaa aaaccattgg tccaccagtc
    45901 gattcctctg tcctttatta cgtctgtata ttttccgttg taagaaatat atggaatttt
    45961 attccccatt ctgtctaatt ccgcatctat cttttcagaa gctacattca aaatttttct
    46021 tgaccattcc ttcgtctgtt cattcatgga tatcccgcca ttcttttaat ttttatgaat
    46081 gtggagttgc tcctagaacg aactagatgg ttaacattcg ttattttttt tacttatttg
    46141 cagctccaat gtcattctcc ttaaatttag actgcgccca cacataaatc aagaccattg
    46201 ggatcactga agttgctaaa attagcataa ttgcgccata attaattggc tcacctgtac
    46261 tagaaaccgt catatttttg ataattaagg gaaccgtata tttatcttta tctgaaagca
    46321 tgactaatgg aaatagatat tgattccaac ttcccataaa gataagtaaa ccactagtaa
    46381 taatcgtagg tgataaaatc ggtacagcga ttttcgtaaa aatcacccac tcattggcac
    46441 catcgattct ggcagcctcc atcagtgctg taggaaattg actgctatat tgtcgcatca
    46501 taaacactga agttgtactg gcaatcgaag ggatgatcac acctgcataa gtattcgata
    46561 atcctaaacg actcataatg taaaataaag gaatcaatgt ggtaaaaaaa ggcaccatcc
    46621 tagatagcat aactgtattg aacaacagtt tattccccat aaaacgatac tttgctaatg
    46681 cataacccgc cattgtagaa ctggctgtag ctaaaactgt actgattaca gcaataacaa
    46741 tcgaattcca aataatcaag ggataattat atctcgccat gatggcttgc cagtttttta
    46801 atagcatatc cccaaacttg aaattgattc cgccacttaa aatttcttga ttactttgcg
    46861 ttgaagctac aatcatatat aaatatggca tcaaaaatat taaggcacca aacacaataa
    46921 tgacgtacgt aaaaattctg ctttgtatct tttgtttagt catttttatc acctccgaat
    46981 ttaaattgaa tagccgataa tataacagat acaaacacca tcgtccaggc aatagcagag
    47041 gcttttccaa aatcaaccat ttcaaaactt gttttatata aattgactcc tacgaccaaa
    47101 gctgaatttt ctggtccatt ggaagtccca aagaatatat ttgggatatt gaaattaccc
    47161 aagccattga tagtggctaa aactgtggcc attaaaatga ttggctttag catggggatc
    47221 gttaataaaa agaattgccg aaatttcgat gctccatcta tttcagcact ttcgtatatc
    47281 tctttagaaa tcccttgaag accagctaaa aataaaatag tgttatatcc tacactaagc
    47341 caaatcatcg tgatgatcat tgatattcgt atccaaaaag gatttgttaa ccactgtaca
    47401 ccgttaattc ctagcgcatt caaaaatcca ttgaataacc cagtcggatt gaaaaaagtc
    47461 atgaatacgg aagctacagc caccgttgat gtaatggttg gaatataata gacagttcta
    47521 aaaaatcctt ttccttttaa ctgtgctgaa ttcaataaaa cagcaaagta aatagctaat
    47581 agcatcgtta ttggaattga tcctgccatg ataatcaaaa cattcgtgta agatttaata
    47641 aacaatggat ctcggaagat agctagataa ttgtcaaatc caacaaattc aaatgagtag
    47701 ccaattttca taaaactaaa agcaaacgag ataatgatgg gaatcaaaat cgtcacaata
    47761 aagaaaataa caaaaggtgt gaccaatcca taagcacttt ttttatgaga cgtatctgtt
    47821 gcttttttct tgctgttctt tattgtcttg cttggtaacg aagattgcat catcgttaag
    47881 tcctcctaat attttttaat caccagttaa ttttacgccg tacttatgag caaaattttc
    47941 tgcttctttt agatatgaat tttcaaaatc acctgtttgc caatagttat atgcagcggt
    48001 agtcaaataa gacgtagcat ctgaataagt ggctggtaaa acagtatttg atgtattttc
    48061 attgatttta ttcaatagtt catagtattt ttgtcctcct tagtagtcaa actttttctc
    48121 agctgcttct gtttgatagg ccaatgagtt agatgtagcc acgcccagct ccagtgcgtt
    48181 ggctaagcat tcttcatctg tcagcataaa tgtaagaaat tgttgtgcca cttgtggatt
    48241 tttggcatct ttagggacat aaaaacttga tcctcctgat actgcacctt ttcctggatt
    48301 ttcttttgaa aaaggaatca gtggagccat gcgccatttg tcagatgaat caggaaaatt
    48361 caaagacata ttcgtagcat accagccgcc agcaacaaac attgcagatt cttgaaatgc
    48421 tttctcagca tcttgtgcac cgtagaaatg tacaatatct gagtcgatca tttctttgat
    48481 taactttgca gcatgtttcg cttctttagt attcagacgg atatttcctt cttcatcaag
    48541 caaagattga ttctgctggg ctagcatgtt gataaacaat tcttggtccc cagattctgg
    48601 caaggcaatt ccaaaactac cagttttttc ttttaatgct tttgccacct ggatatattc
    48661 ttcccaagaa ctgatttctt caaaatctac acctatttca gcaaattttt ttggttgata
    48721 ataacaaagt gctggggtaa aatcactagc ccaaggaata atcgtttcat tactcgtttg
    48781 ttgacgcaaa atattcaatt tttgatcata aaactgatcg ccatatttgt cataaaatcc
    48841 atattcatca gcactataaa aagcatcttg aaatttttcc agcacacctg ctgctgaagt
    48901 gtctgccatg gagataatat ccggcatatc ttcgtgtgtt accaattttg gggtgatcat
    48961 ttgtactgtt gctgttcccg aagctacatt cacaattttt aagtcaatat ttgctccagt
    49021 tttgcggtta tagatttttg cagcattttc aacatagttt gtcagtttgc cgtttgcgac
    49081 atatacttct aatgtagtgg ctttttttat cattggatct gcgatgtttg ctgttacttc
    49141 ttcgttagtt tttccacatg cacttaagct gaataatagg gtaatgttta gcaaagctat
    49201 caccaatttg tatctttttt tcatgtctat cccttctttc ttttcaagtt cttatataaa
    49261 acgctttcac ttatgtatat taacggaatt agtaacacaa atatttatat aaaaaaatat
    49321 tttacttgtc gttttggctt ttcatttttg ttataatagt cattaattat aaatagaaag
    49381 ctttttatat aaaaagagaa aggaaagtgg ttcttttgtt aacaaacaac caatcctcca
    49441 atcttgtcaa aaaaacacta acctattcca tagataccca tcttcattta tctgctgctc
    49501 cctattttcg actactaata aatgaaagcg ctaatttata tgctgaattt gcacataaat
    49561 ctatagaact agataatcct tcccttttat tcattgataa acagcaagaa gtccaaatct
    49621 tttctactca aaaggaaaca caaactttta tcattatcca gttttatttt gatgagcaat
    49681 tattagcaca tattacggga caatcttttt ctatagatca agaaataaca tctatcatta
    49741 cttttctgaa aaatgaacag ttcaaaattg attatctaaa acatagtaag ccagctattt
    49801 cctatgatca ggaaatcaat tcttttttca tactatgcca acagatgagc attcatttgc
    49861 tttccttatt agttacttat tgtctgcaaa aaattcaatt gtcttcctta ccaaggcatt
    49921 atcaacagga acttgcttta ttgacaatac cttcaaatga gaaagtcatt cctaaaaaaa
    49981 agcatattct ttattcagat gaattagttg atccaatcat acgatattta gaagaacatc
    50041 tttcagaaaa ttttttgctt gaacaattag cccaaatttt ttttatcagt ccagccagct
    50101 taaaaaaaca ttttagaaaa gtgaatggaa aaagtgttat tagctatttt aaagaactaa
    50161 aaatggaaaa agcaaaacgt ctcgtgctag aaaatgaatt aacctataca gaaattgcag
    50221 atatattagg atttcattcg atccatcatt tttcttcagc ttttaaaaaa tatacagggt
    50281 tatccccttc aaagtattat ctatccttaa aatagaaaaa aatttttagg ttttatccac
    50341 aaattatttc tctatatttt ctgtctattc aaaataaaca ataattttcc ggcacaatta
    50401 tttgctgttt tttaccataa catagtacga tcaacgtaga aaaggagatg tttttatgaa
    50461 agttgttgtc attggcggtg tagctggcgg tccttcattt gccactcgtt tccgtcgatt
    50521 gaatgaagcg cacgaaatca ttatctatga acgcggagaa aatatttctt atgcaagttg
    50581 tgctttgcct tattatttag gaggtgtgat cactgaccgt gactcgctga tcgaacgtac
    50641 accagaaata ttgaaaacaa aaaacaacat cgatgtcttt actaaacacg aagtaacagc
    50701 aatcgatcct tctactaagc gattaacagt taaagaccta tccacaaatg aagaaataaa
    50761 aacagattac gataagttga tcatctcttc tggtgctaga ccagattatc cggatattcc
    50821 cggagttttt gaagcagaaa acggctttgt cctccgtagt gtgacggatg cggatcgaat
    50881 caaatcgttc cttgaagaaa aaaatccaca acatgtcgtc attcttggtg caggtgttat
    50941 gggtctggaa ttagctgaga atttcaagca tcgcggctta gacgtgactt taatcgatca
    51001 attgccacaa gtcgctttcc cttatgatcc agaaattgct aatttagttt atgacaaatt
    51061 gctgaaagaa ggattagccg ttcatttaga aacaagagtt actgagatcc gtgataaagg
    51121 tcgagaaata gtattatcag atggctccgt cctttctgct gatatgctaa tttttgctgt
    51181 tggtgtttct ccgaagaatg aagtggtgaa agcaaccggc atacaattat cagatacagg
    51241 tcagatcatt gttgatgacc agttacaaac caatctgccg gacatctatg cgattggcga
    51301 tattatcgaa acgactagtg tagtgactgg tcagccgatc caaagtatgc tttccagtgc
    51361 ggccaatcgt caaggacata tgctggcaga tattttaaat ggtacgccta tgcgctatcg
    51421 cggatatatt ggtgcaggtg tcgcaaaaat ctttgatcat acagcaagtt atgcgggaat
    51481 gacagaacat gaattaaaag catcaggcat aacaaattat aaaactgttt ttatcactcc
    51541 ctttgaccat gcctatttct atccagaagc tacaagatta aatctaaagc tgatttttga
    51601 tgcagatagc ggtcgtattt taggtggaca agcatttgga gaaaaaggtg tcgataaacg
    51661 gatgggagaa ctttctgtag caatcaccgg aaacttgaca gtctttgatt tgcctgattt
    51721 ggagttgcct tactctccac cgtattctac tacccgtgac ccgttgaata tagctggtta
    51781 tgtcgcaatc aatcaaatga cgaatatcgt agaaacgatc aaagcaagtg atatacccga
    51841 aaacgatttg aaagaagcgt tctttttaga tatacgtgaa cctaataaag caccttctgg
    51901 cagtatctcg gcaacaaaaa atattccaat gaatgagtta cgagatcgaa tcaatgaaat
    51961 cccaaaagat aaaaaagttt acattacttt cagaagagga ttgaatactt atacttctgc
    52021 ccgaatcttg gcaggtttgg gtatcaaagc gattttgatt gaagaataaa aaaacgaccg
    52081 cggtgggtag ccgcggtcta ggagttattt tt
//
LOCUS       DARBEC010000006        51219 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.006, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000006 DARBEC010000000
VERSION     DARBEC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.006"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            330..1175
                     /locus_tag="SCL80_000356"
     CDS             330..1175
                     /locus_tag="SCL80_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1106249.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="SCL80_000357"
     CDS             1456..2676
                     /locus_tag="SCL80_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEG1106250.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="SCL80_000358"
     CDS             2673..5393
                     /locus_tag="SCL80_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1106251.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="SCL80_000359"
     CDS             5377..6321
                     /locus_tag="SCL80_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1106252.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
     gene            complement(6390..7400)
                     /locus_tag="SCL80_000360"
     CDS             complement(6390..7400)
                     /locus_tag="SCL80_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1106253.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(7580..7924)
                     /locus_tag="SCL80_000361"
     CDS             complement(7580..7924)
                     /locus_tag="SCL80_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1106254.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(7917..8351)
                     /locus_tag="SCL80_000362"
     CDS             complement(7917..8351)
                     /locus_tag="SCL80_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEG1106255.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            8489..9226
                     /locus_tag="SCL80_000363"
     CDS             8489..9226
                     /locus_tag="SCL80_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106256.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            9219..10430
                     /locus_tag="SCL80_000364"
     CDS             9219..10430
                     /locus_tag="SCL80_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106257.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            10634..11416
                     /locus_tag="SCL80_000365"
     CDS             10634..11416
                     /locus_tag="SCL80_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEG1106258.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            11486..12148
                     /gene="trmB"
                     /locus_tag="SCL80_000366"
     CDS             11486..12148
                     /gene="trmB"
                     /locus_tag="SCL80_000366"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEG1106259.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCL80_000367"
     CDS             complement(12246..14000)
                     /gene="ade"
                     /locus_tag="SCL80_000367"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEG1106260.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNKQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            14132..15061
                     /gene="tadA"
                     /locus_tag="SCL80_000368"
     CDS             14132..15061
                     /gene="tadA"
                     /locus_tag="SCL80_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEG1106261.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGTVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLKLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            15018..16067
                     /locus_tag="SCL80_000369"
     CDS             15018..16067
                     /locus_tag="SCL80_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEG1106262.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            16064..16342
                     /locus_tag="SCL80_000370"
     CDS             16064..16342
                     /locus_tag="SCL80_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEG1106263.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            16317..16787
                     /locus_tag="SCL80_000371"
     CDS             16317..16787
                     /locus_tag="SCL80_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEG1106264.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            16804..17106
                     /locus_tag="SCL80_000372"
     CDS             16804..17106
                     /locus_tag="SCL80_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106265.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            17123..17494
                     /locus_tag="SCL80_000373"
     CDS             17123..17494
                     /locus_tag="SCL80_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEG1106266.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            17466..17825
                     /locus_tag="SCL80_000374"
     CDS             17466..17825
                     /locus_tag="SCL80_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106267.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            17997..19004
                     /locus_tag="SCL80_000375"
     CDS             17997..19004
                     /locus_tag="SCL80_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1106268.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            19029..20213
                     /locus_tag="SCL80_000376"
     CDS             19029..20213
                     /locus_tag="SCL80_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEG1106269.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(20650..22233)
                     /locus_tag="SCL80_000377"
     CDS             complement(20650..22233)
                     /locus_tag="SCL80_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1106270.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVDINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHCQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(22811..22954)
                     /locus_tag="SCL80_000378"
     CDS             complement(22811..22954)
                     /locus_tag="SCL80_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEG1106271.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(23176..23655)
                     /locus_tag="SCL80_000379"
     CDS             complement(23176..23655)
                     /locus_tag="SCL80_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1106272.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(23728..24012)
                     /locus_tag="SCL80_000380"
     CDS             complement(23728..24012)
                     /locus_tag="SCL80_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106273.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(24090..24284)
                     /gene="ssrS"
                     /locus_tag="SCL80_000381"
     ncRNA           complement(24090..24284)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="SCL80_000381"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24356..25642)
                     /locus_tag="SCL80_000382"
     CDS             complement(24356..25642)
                     /locus_tag="SCL80_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEG1106274.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            25780..26451
                     /locus_tag="SCL80_000383"
     CDS             25780..26451
                     /locus_tag="SCL80_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEG1106275.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            26417..26905
                     /locus_tag="SCL80_000384"
     CDS             26417..26905
                     /locus_tag="SCL80_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEG1106276.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            26918..27865
                     /gene="prmA"
                     /locus_tag="SCL80_000385"
     CDS             26918..27865
                     /gene="prmA"
                     /locus_tag="SCL80_000385"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEG1106277.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            27866..28630
                     /locus_tag="SCL80_000386"
     CDS             27866..28630
                     /locus_tag="SCL80_000386"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEG1106278.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            28863..31076
                     /locus_tag="SCL80_000387"
     CDS             28863..31076
                     /locus_tag="SCL80_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEG1106279.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            31087..31533
                     /locus_tag="SCL80_000388"
     CDS             31087..31533
                     /locus_tag="SCL80_000388"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEG1106280.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            31704..32963
                     /locus_tag="SCL80_000389"
     CDS             31704..32963
                     /locus_tag="SCL80_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEG1106281.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(33052..33999)
                     /locus_tag="SCL80_000390"
     CDS             complement(33052..33999)
                     /locus_tag="SCL80_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1106282.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            34393..35685
                     /locus_tag="SCL80_000391"
     CDS             34393..35685
                     /locus_tag="SCL80_000391"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEG1106283.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            35700..37469
                     /gene="aspS"
                     /locus_tag="SCL80_000392"
     CDS             35700..37469
                     /gene="aspS"
                     /locus_tag="SCL80_000392"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEG1106284.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            37578..38453
                     /locus_tag="SCL80_000393"
     CDS             37578..38453
                     /locus_tag="SCL80_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1106285.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            38719..40602
                     /locus_tag="SCL80_000394"
     CDS             38719..40602
                     /locus_tag="SCL80_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1106286.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(40677..40961)
                     /locus_tag="SCL80_000395"
     CDS             complement(40677..40961)
                     /locus_tag="SCL80_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1106287.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(40951..41685)
                     /locus_tag="SCL80_000396"
     CDS             complement(40951..41685)
                     /locus_tag="SCL80_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEG1106288.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            41814..42437
                     /locus_tag="SCL80_000397"
     CDS             41814..42437
                     /locus_tag="SCL80_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEG1106289.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            42629..43750
                     /locus_tag="SCL80_000398"
     CDS             42629..43750
                     /locus_tag="SCL80_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEG1106290.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            43757..46885
                     /locus_tag="SCL80_000399"
     CDS             43757..46885
                     /locus_tag="SCL80_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEG1106291.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANERIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            46904..50098
                     /locus_tag="SCL80_000400"
     CDS             46904..50098
                     /locus_tag="SCL80_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEG1106292.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(50244..50951)
                     /locus_tag="SCL80_000401"
     CDS             complement(50244..50951)
                     /locus_tag="SCL80_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1106293.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
BASE COUNT        16763 a         8942 c        10637 g        14877 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc ttgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgttttcttt tattttgatg aatcagttgt ttcttcagtt
     6421 gaagattctt ttgttgaatc agttgatttt gtgtcagaac tctttgttga agctgtagtt
     6481 tcacttgagt ctttcgtact tgatgaagtt gttgtgaatg cactcaacac attttcaaag
     6541 gcatcatctt tgatttttac atttgcatct tttaattctt caccgattac tttagttgtg
     6601 aatgcattgt cacttagttt agtttcagta gcgatatctt ttagttgatc tttgtattta
     6661 tccatgtcat tgcctttatt ttggtttttc accattttga caacgtagta ttctgtcgca
     6721 taagaagtag gatttgttgt agtaataaca tcggagatct caccgtcttt caatttgaaa
     6781 gctgcttctt ttacttctgc aggaatagtt gttgtcgttg agtcgaattt aactttaccg
     6841 ccatcttctt ttgtctctgt atcagttgat ttgtctttgg ctaatttaga gaaatcatct
     6901 ccatcatccg ctgatttttt cacatctttt gcttcatctt cacttgataa tttgatgatt
     6961 tgtgcttcta cttctggatg gaaagatttc caagcagttt tcaaatcgtc atctgtaata
     7021 tctacgtgag ctttcaagcc agcttcaaaa gctaaattgt tacgaatata ttctttataa
     7081 gtatctttcg tatatccagc agcttctaat tgtgattcaa aagtatcacc taatgatttc
     7141 gcttgtttgt cgtattctgc atccacttgc ttatcggtta ctttatcgcc atatttgtta
     7201 ttgaatactt tataaatgat catccgttgt actaaggatt ggttcgatga ttcaagtttt
     7261 gcttcatcat agaaatcaga tacagtgatc gttccgcctt tcatcgttgc gatatcttta
     7321 ttagtatcgc ctgaacaagc tgctagagtt aaaacagcca acgcactagt agctgccaaa
     7381 ataattgatt tttttttcat taaaatttgc actcctgtaa ttagttatta tttttatata
     7441 ttaactatac catatcccat ttcctttaaa aacgttgtcg tggtagtttc aaacaaaatt
     7501 caaaatttca tcacattcct aggaaatacg caaacttttc tcacttatat agttaaatct
     7561 gttgagaaaa taatcattat tatatttgtt caggatactc tggcagatca gcttttagtt
     7621 tttctacttg ttcattgatt tgtgtgatac gtggttcggc ttggaattta aaatcttcga
     7681 tatctttttt gattgcttct tggaaagcag gaatcacaga ttcagctgtt tcttttgttt
     7741 cgatcaatgc gcttttaaaa tgattcaggc tgttatttaa atcatttgtc agagttgttg
     7801 cttcttccaa ttcttcgatc agtttctttc ttgtttcatt cccgctcctg ggcgccaata
     7861 acagacctcc gacagtgcca gcagcagctc caaataacag tcctttgaaa aagtttttaa
     7921 tcatcgcagg acacctgctt tacgattgtt tcttgaatag cttttagttc ttccgatcca
     7981 taactatctt gatgattgac aaagtgaata gaaaaatcat cctctttgct gtagcgaggg
     8041 actaaatgga tatgtgaatg aaaaacagat tgataggcta gttcacggtt gttattcaag
     8101 atattaagac ctttcatttc tggaaaagct ttttccaatg cttgagcaat cttaggcaca
     8161 cgcgaaaaaa cctctcctgc aagttcggga tcatattcaa aaatatctgc tacatgacgt
     8221 ttaggcacaa ctaatgtgtg tcctttcgtt gcttgagaga tgtctaaaaa agcatagacc
     8281 ttgtcgtctt catatatttt atagcttggg atttcttgat tgatgatttt gcaaaaaata
     8341 cagttttcca tataaaacat cctttctata aagaataaat acttcagctt aactttacca
     8401 taagttaaag ctattgtaag gaaaaaaaca aaataccgtc attttcttcg tggtataata
     8461 aataaaaata aatggcggag gaaaaagaat gagtttaatt atcgatcatg tcacaggggg
     8521 gtacggacat ctccctgtgt taaaagatat cagtttcgat gtacagcctg gagagatagt
     8581 agggttgatt ggactgaacg gagcagggaa aagtacgacc atcaagcaca ttatcggtct
     8641 gctggaagct tcaaaaggaa cgatagaaat caatcatcta tccttaaaga aaaatacgga
     8701 agaatatcgc aaacaaattg ggtatattcc agagactcct tctttatatg aagaattgac
     8761 attgagagaa catatcgaag tcactgcgat ggcctacgat atacctatgg atatagcaat
     8821 gaaaagagca gaaaagttgt tggagacttt tcggttatct aatcgtcttg actggttccc
     8881 agctaatttt tcaaagggaa tgaaacagaa agtgatgatc ttatgcgcct ttttgatcga
     8941 gccttcttta tatatcatcg acgaaccatt tttaggcttg gatccactag ctatcaatgc
     9001 attgcttgaa ttgatggtag aaatgaaaaa agaaggagcc ggtattttga tgtctacgca
     9061 tattttagct actgcagaaa aatattgtga tcgttttgtg gtcttacata acggagagat
     9121 acgagcagac ggttctcttg agatgttgag aaaagaattt cagctgccgg attcttcttt
     9181 ggacgaaatc tatattgctt taacaaagga gcaggcacat gagtagtttt tttcaaaaac
     9241 gtttagcaag acatcaaaag aaaatgatga aatatatgcg ttatgtattg aacgatcatt
     9301 ttgttttagt ttgtcttttc ttgattggcg gtgtgggatt atattattct aattggttga
     9361 aaaccctgca gccaccgttt gcaattggag ggctcataat ctctgtattt tggatgctct
     9421 gtttattcgt gggtaagatg gctacttttg cagaggcagc agatattgtt tttttacttc
     9481 ctaaagaaaa agaaatgcaa acctatttgg aacgagcttt tcgctattct tgcatttttc
     9541 cgttgatctt tttgatattg gcatgcggat tggccatgcc attagtggtg gttagtacgg
     9601 agcaatcctt ttcgatgttt ttcgtctatc tgatcatatt atggagtttg aaattcagtc
     9661 atctgcaaat caagagagca gaattgtttc gactagatca acaagttatt cgttattgga
     9721 aattaggctg gtttttttct tctctgatct ttttgtttct atcattttat tggctaatat
     9781 gggcaggaat agcaggtgca ttagtacagg caagtctata ttactggttt ttgtggaagc
     9841 aacaagaaaa aggattggat tgggagaaaa tggtgcatgt agaagaacat aggttgcatc
     9901 agatctatca atttatcaat ttattcacag atgttcctga aattacggca aagatcaaaa
     9961 gaagacgcta tttggatcct atattgactc atattaaaag agaatcaaaa aacacttatc
    10021 tttacttgtt tgcaagaaga ttggcaagag gttctgattt tagtggtctt tatttccgat
    10081 tagtaattat aggtgggtta ttgattgccg gagtagtcga cttttatttt agtctgatta
    10141 tcggtttctt atttttatat ttgatcggtt tccaactgtt acctttagcg aaccagttcc
    10201 gctatatggt tctcgttcag ctttatccaa tccataagaa tcagaagaag caggcgattc
    10261 agtatctact cacatgggta ttaattatcg cgtcggttat ttttggtttg attgctgcag
    10321 tcgtattaga tgggcagcag cgatgggttc cgctagcggc ttatttgatt gttactatgc
    10381 tctttgttaa actatatgta ccaactcgtt tgaagaagat gtcagactga aaacaaagct
    10441 gtgtagggta aaaatgagaa tgaaaaatag agcgttgaca gtaagaatgc tgttcgttct
    10501 attttttttg agaaaagctg gttttttgaa tggcaattaa atgaaaaaga acgtatatgt
    10561 gcttgacgtg tagtgttcag gcgactagaa taataagtac tgcaaaacaa agagtaagag
    10621 gacgggagaa tgcatgatgg agtttcagtt agaccaagag tggcggttac gtcccatcaa
    10681 aggcgacaca ggaaaaacat acataggcat gcgtgatgag gacaaagtct ttattaaacg
    10741 aaacacgact ccgatgttgg ctgctctttc aagggaagga attacaccaa aacttgtttg
    10801 gacaaaacga acggggaatg gcgatacttt gacagcacaa gagtggctgg aaggtcgctt
    10861 gctgactcct aatgagattg gtcaaagaaa cgacgtgatt gatgttttgt accatttaca
    10921 tcattcaaat tctcttaaaa ccatgctgac ccgtatcggt ggtcgtgtca tgacgccgga
    10981 gaagatgctg caaaaatatg aaaaagaatt accggaagaa ctccagaaaa atcaatttct
    11041 gcaaattgtc catcgttttc ttcgtgagca gatcccgtca atagataagg aaaacttatc
    11101 agtagtacac ggagatgtaa accatcgtaa ttggttagta tgtaaaaatt atttatatct
    11161 agtcgattgg gattcaatca tgtttgcaga ccctgcggtg gatattgcaa cgattttagg
    11221 aaattatgtg ccactgtcta gctggaatca gtggctgatc agttatggca tacgcccgac
    11281 tgacgaaatg cttgaaaaat ttcattggta tgcggccatg aatttgctcc aagaaatcac
    11341 cagatattgc aaacgtgaag atcatcgacg gatgaacgaa gaaattttac aattaaaacg
    11401 aattttcagt ggctgaggtt tggcccaaac tctgcggaga tttggccaga cctttttaat
    11461 tagaggaaag gaatggagat aaataatgcg tgtccgaaat cgtccgggag cagcggaaat
    11521 gttagcggct cacccgaatt ttgtaattag tgatccaaca ttgtggaagg gaaaatggaa
    11581 tgaattgttt gaaaacgatc atccaatcca cattgagatt ggtatgggga aaggccagtt
    11641 catcactgga atggcaaaag cccatcctga aatcaattat attggagtag aaatgcaggt
    11701 cagtgttgtt tcgattgcac tagacaagtt gatcgaacaa ccgcttccta acctgaaact
    11761 tctacatgtg gatggatcag cgttgacaga gtatttcgct gacagcgaag tagatcaaat
    11821 ctatctgaat ttttccgatc catggccaaa aaaacgtcat gaaaaacggc gattgacata
    11881 taagactttt cttgctgtag atgaacagat tcttcgtcca aatggagaaa tccatttcaa
    11941 aacagataat caaggattgt ttgagtattc tctggcaagt ttttctcaat atggcatgat
    12001 cctcaaacag gtatggttag atttacatca aagtcaattt gaaggcaata ttatgacgga
    12061 gtatgaagaa aaattttctt caaaaggtca acgtatttat cgagtagaag ctcaatttca
    12121 agataagacc aaaaagaaaa gtctgtgaaa ctaactttct gctttcataa aaacattaat
    12181 atccataaat aaatctcaga aaatgaacat tacagatgta atgtattttc tgggatttat
    12241 ttgatttaaa atgtatttat ttcatcggtt tttccacaag gtggaatcaa ttggaagcga
    12301 gaaaatgaaa ataatccttt gtcggtgatc ttcaattcgg gtatgacaga taatgttaag
    12361 aaagaaagtg tcaaaaaagg atcaaaggtt ttagctgctc ctagttgatg tgctttagtt
    12421 attaattcat tcaactgttc atagacgatc gaaaaaggtt cctgtgtcat gattccgcct
    12481 ataggaagag aaacacaagc tatttcctgc tgtttgttga caacggtcat cccgccacct
    12541 ttttgaatca aaagattagc agcatacagc atatcctcgt cgtttgtccc aacgacaact
    12601 agattatgcg agtcatgggc aatcgttgtc gctatcgctc cttgtttcag ctgaaatcct
    12661 ttcactatgc ctaatccaat gtttcccgtt tgatggtgac gctcgatcac cgcaatcttt
    12721 aattggtctt tttcgattga aggaatgaat cgttcgcctt gtcggtccac tttttctacc
    12781 aaatctttag tcactagact attcgggata atttcgatca catgggcttg tgaagaagaa
    12841 agaagcagct ctaacgaatg tggatttaat ggttgagcgt ttagttttgg cagacaacac
    12901 gtatatgaat ttgaatgatc agcaaataaa tggtcttgga gttgcccaga ttgaacaact
    12961 aatgttccct tatagaaaac ttcatgaatt ggcagcttgg taagatcgtc tgttaagaag
    13021 aaatcagctt gatacccagg ggcaatcgca cctagtgttt tcaaatcaaa acattctgct
    13081 gcattcaatg ttgccatctg aatggcttgt aatggatcga ttccaagtgc aatagcttga
    13141 cggacgatat gatcgattga tccttctttt actaaatcat tgatcagttt atcatcggtg
    13201 acaaagaagc atcttcttga attttcaggt gtgatcgccg gtagtagcga tgccagatct
    13261 tttgcaacgg tcccttctcg aaccattaaa tacataccaa gatccaaacg ttctttagct
    13321 tcttttgcag ttgtagcttc atgatccgtc cgaatacctg ctgcaggata aacatttaac
    13381 tcttccatgc cgataccagc tgcatgcccg tcaatttttt tcttatgctg gtgcatcaat
    13441 tggatctttt ctaggatgtc tgtttcatta tttgctactg cttgatagtt catgacttca
    13501 gctaagccta aaacttcggg tttttgtaaa aaaggggcta atttttttgc atttaaattt
    13561 gcaccattgg tctcaaaagg ggttacaggt acacaagaag gtagcataaa aaagatattc
    13621 atgagtgttt gtcttgcatc ttctatcata aactcaagac ctgtactccc agcaatgttt
    13681 cctatttcat ggggatctgt cactacagta gtaactccat gaagcaagga tacttttgaa
    13741 aattcgcttg gtgataaaag tgagctttcg atatgcacat ggccatcgat gaatcctggc
    13801 actagatatt gcccattagc attatgtacg ctttttcctt cgtattcacc gattcctacg
    13861 atcattcctt cgcagattgc tacatctttt tccatgattt ctttcgtaaa gacgttgacg
    13921 atcttagcat gcttgatcac taggtcagca ggcttttttt tacttgctac ttcgatatat
    13981 ttccttaatg ttgtttccaa tggagtgcct cccaagataa atttttataa gtataacata
    14041 aattcggctt ttgccaatat ttttttgtaa attcatttta tttgaaacaa aaagatcaaa
    14101 aagacgtatg ttttttgtgg aggggagaat aatggaaata aaacgattat cggatcaaat
    14161 gataagtgaa agttttgcca aacatgccca agacgtatat atattgccag cttcagaagt
    14221 attttacatc tattttcgct atggaaaaaa acgaacattc tggaagaaag tctcttcggc
    14281 agatggacag caactgatct caagatttaa gtatttggga cagatggata tcggagaaaa
    14341 aagaaaagct cagttaggtg catttactta ttctttagaa aaacaggaag ttcgtctgcg
    14401 gttgtcttct gtaggagatt accttcaacg agagagttta gtgattcggc tattatacgg
    14461 tacttttgaa acaccccatc gttgctttag ttctcgagat tttgcaacta ttttacagaa
    14521 aaccgtcaga cgtggattat accttttcag cgggacagtc ggatcaggca agacttctct
    14581 aatgtatcgg ttggctttgg aagaaaaatt gcaagtgatt actatcgaag atcctgtaga
    14641 gatagaagaa ccgttttttc tgcagctaca gatcaatccg aaaatagcac agacttatga
    14701 tacacttttg aaactcgcat tacggcatcg tccagacttg ctaatcattg gcgagataag
    14761 agatgaatca actgcacatg ctgcggtaag agcagcacta acagggcacc gggtttttgc
    14821 aaccgtacat gcacgcaatc ttgaaggtac attggtcaga atccatgaac tgacaaagaa
    14881 aaaagaagag ctatctgaat gtttagctgg tgtcatttat caagaacttt tggaagatgc
    14941 caatcaccat ccttctgttt tatggagtta tcaatttatg gaggaagaat cagtaaaaaa
    15001 atcatgggag gaaagttatg aagaagctca ggaaaatatt tggcagtcag aactattcta
    15061 aaggattaac aagaagccag caaaaccagt ttgcacaaac catgggagaa ctgcttttat
    15121 ctggttttag tctgcaagaa tccctttatt tattactcac gatccgttcg tttccacctg
    15181 ttattttggc aaatatccaa catcagctaa aggaagggca agcgtttagt gaggtacttt
    15241 cgcaaatagg atatggagca gagcaactgg tacagatcga gttagctgaa cgtcatggga
    15301 atctgccgac aactttgttg gaaattgcca aacagtttcg gcttgttgaa ggatttcgca
    15361 atgatttgaa aaagctgctt gcatatccga tgctattgct cacctttttg ctaggtatct
    15421 tgtttagtat gcggttgttc gttttgcctc agcttattgg ttcaggaatg gtcatgcaag
    15481 agcattgggg tattcagctg atacaagtct ttcattggta tgttctacta ttcatcctag
    15541 cggttactct attggcttta ctagtaaggt atcgtctgat gaagttgtct cctgttgatc
    15601 agagtgaatg gataagcaga caattattga ttggaccgat atattccttg tatcaatcgg
    15661 cttatttgtc actagagatt ggaaagcttt ttcatgaagg tttcgaatta aggcaaatca
    15721 tagtctgctt aaaggaaacg aaacaagaaa gtctgattca gcatcttgcc tctaagttga
    15781 ttcaaggatt ggaggcaggt atgtcattgg ctgagcagtt ccagaattat cgttttctat
    15841 cagaagaatt cagtaaaatt gttttacagg gagaggcaaa gggaagttta ggtaaagaac
    15901 tgatttttta cagcgaatgg acaagaaaac agcttttcaa acgtcttcat cactggatgc
    15961 attttattca gccaatcatt tttttgggcg tagcggcttt gattttatta gtttatgccg
    16021 ctgttttatt acctgtttac ggaagcatag aggaggtttt gccatgaaaa agagaaaaat
    16081 atcttattat agcggtttta cattgatcga aatgctaatc gtcttactga tcatcagtgt
    16141 attagtgttg ttgttcgtcc ctaatttgtc gcgttatagg aatcatgtag accaagaaag
    16201 tcgtgaagcg atcatccagt tggtagacac acaaaaagag ctctatgcgc ttcaaaataa
    16261 tggaagagtt cctactgtgg aggaattgtt gaatgaaggc tatatcaaac gagaacatgc
    16321 agaaatctat cagcggcctt gaaggtaagg ttttagcatc aggatatacg ttgtttgaat
    16381 cactcttact gattttatta cttacaacga tcctttcttt tccagttctc gcattttctg
    16441 catggaagaa tgaattggca gtctatcaat ttttcggcca atttgaaaaa cgaatctatt
    16501 ctacgcaaaa aatcgctatt gtgaatcaga tccaaacagg cttttattgg aatagtgaag
    16561 agaatcaaat catttttcat gttccgaatc cagataaaat aaattgggaa gtattagata
    16621 ttcctgatga gattaccttg aaacgccatg cttctattac ttttgctgct agaaccggga
    16681 atgaaagttc actaaaagct tatcaatttt attgggaaga aaaagagcgg acgattacgt
    16741 atcaattcca gatagggagt ggtcgctata caaaaagaat tgaataaaac aggatttgtt
    16801 ttgttggagt cccttacagc gcttatgatc gcttctgtct gtgtctactt agtgagcttt
    16861 ggccagctgt ttttgcttca ggcagaaaag aaaagtgggg aggaactgct atttattcga
    16921 atgatttatg aagatatcaa gacgcagcgg atgtatcaca aaagaccaga gcgaagggaa
    16981 gaaatgatcc catacgatat ccaaattgtc gataataatg gagtaatcga aaaagtgttt
    17041 gtcaatatgg agaaagaagc gatcagtatt gaaaaaacaa gtaattccag catttacgct
    17101 gattgaatgt ctaattggac tgatgatatt gagcagtcta ttgctcacat tcaatctttt
    17161 gattcagcaa accatccaag taagtgaatt tttgcagcgt aaagatcaaa aagagtggct
    17221 aatattttta gcacaattag aagaagaact caagagcagt cacaatgtct ctgtgaaaaa
    17281 tcagcaactg cactacacga tcgaggataa acagtatatc atcgaaagat atcaggcaat
    17341 gatacgtaaa agaagaactt ccggcggaca tcaacctatg ttaacaagta tcactgagct
    17401 tcaattattg gaaaaagaac acacaatttc tttttatgta cactttgaaa atggagaaga
    17461 tggatatgcc atatggacaa aaaacgatca gtagaaaatg ttataagggc tctattttat
    17521 tttctgtctt gcttttattt cttttatgta cgtttttatt gatggcagca ttagaaagct
    17581 accgtttgtc aatggatctt aatatacgaa tcaaaaatta ttatatagca aaaataatga
    17641 agcagatggc tttatcggaa cagtctacgc ttgccgaaag ttcggaagga gttttttact
    17701 acacaactgg atctgtaaca tatcagtggg tacaacagtc cctattctta gaggtggaag
    17761 tctcaccgtt tatctttcgg ttcacagaag aagtaaaaaa tgatacagat actagtacag
    17821 aatgatggaa tgttcgtgaa aacgccctct tttctgttat aacactgttt ttaataaaaa
    17881 aatgtttgcc gtttcacaaa taaaaaacag tgaaaacgat tgaaattttg tcaattgttc
    17941 gatagaatta aacaggtgaa aaaattgtta taatatatct agaaatgagg tgcaccatgt
    18001 taccagaaaa aatggaacaa gggtttaatc aaaacctgga agctattcag cttttacaga
    18061 atgcattggg aacttccttt ttagaagcct atgtcgaaaa tgcagaaaat ttaatagatg
    18121 attatcaagt acgtgtggtg gatggagtgc cgacaaaaga gacaacgcaa cggatcacag
    18181 ctctttatga agagttgaag aaactttctt tcgagccaga agaatggcgc agactgtctc
    18241 aattactttt attgaagggc agccagacag aacatttgca ggcgaaccat caactcactc
    18301 ctgatagtat cggtttttta tttgtttttc ttattgaaca actatacaca aataaaaaag
    18361 caccggttaa gattctggat attgcggcag gaatgggcaa cttgttatta acagttcttt
    18421 taaacctatc gaatgcgggg taccaaacag aaggaattgg tgtggatatt gatgatacat
    18481 tgttggctgt cgcagcttct acaagtgatt tgacacaagc aaatgttcaa tactttcatc
    18541 aagacgggtt gcaggaatta ttaatagatc ctgtcgattt tgcaatcagc gatttgccaa
    18601 tcggttatta tccgaatgac gaaaaagcaa aagagttttt aacaagtacc gaagaaggcc
    18661 atagttatgc acatcattta ttgctagagc aatcaatgaa atatgtgaag ccggatggtt
    18721 ttggtttgtt tttgatgccg agtggatttt tagaaacgga tcaaagtgaa gaaatcaaaa
    18781 aatggtttaa agaagaaggt tatcttcaag gaatgattca attgccagat gaattgttcc
    18841 gtaataaaca atcgcaaaaa agtattttga tcttacagaa aaaaggacca caagcacaac
    18901 aagtaaaaga agtcttgctt gtgaaacttg cttcattaaa agaaccagag aaggtgacgg
    18961 aatttttcaa cgagtttaaa aactggaaat catctgctct gtaatcattc aacgagaagg
    19021 agagtcatat gtctaaaaca attgcaatca atgctgggag ttcaagtttg aaatggcaat
    19081 tatatcaaat gccagaagaa accgtagttg cgaaagggat cgtcgaacgt atcggtttac
    19141 aagattctat ttttaccatc aagtacggcg ataatcaaaa attcgaacaa atcatggata
    19201 tcgaaaacca cgaagtagca gtaaaaatgc tgttagatca gttgattgaa ctgcaaattt
    19261 taagctctta tgatgaaatc accggagtag ggcaccgtgt tgttcacgga ggagaaactt
    19321 atggggattc agtagtgatc gatgagaaag taatggaccg cattgctgaa ttagcagaat
    19381 ttgctccttt gcataatcct gcaaacttaa tggggatcaa agcgtttaaa aagattttac
    19441 ctgacatttt gagtgtggct gtattcgata catctttcca ctcaacaatg ccaaagcaaa
    19501 attatttata tagcttaccg gtgtcttatt atgaagattt caaagtaaga aaatatggct
    19561 tccatggcac aagtcatcgt tatgtgtcgg aacgtgcggc aaaattattg aacaaaccga
    19621 tcgaagaact aaaaatcatc acttgccact taggaaatgg tgtatcgatc acagcagtcg
    19681 atggaggtaa atccatggat acttctatgg gattcacacc attagctggg gtgacaatgg
    19741 gtacacgttc aggtgatatc gatccagctg ttctcccata tttaatggaa aaattagaga
    19801 ttgatattga tgaaatgatc aatgttttga acaagaaatc tggtctgcta ggcctaacag
    19861 gattgtctag tgatatgcgt gatcttgaaa agaactatga aaaagaacat attcgtttag
    19921 cttatgacat tttcgtggac cgcatccgaa aatacatcgg cggttatgtg acaacgatga
    19981 atggtgttga tgcaattgtc tttactgctg gtatcggcga gaatgacgga catgtacgta
    20041 gtgaaatcat caaaggtatg tcttggtttg gttgtgaaat cgatccagcg ttgaatgaat
    20101 tgcgtggtga agaagtagat atctctacaa aagaatcaaa agtaagagta ttagtgatcc
    20161 caacagatga ggaactaatg attgcccgtg atgtggagcg tctgcgcggc taagccaaag
    20221 cattcgttag gataaaagat gaaacgcatg caagagggaa ttcttgcatg tgtttttttt
    20281 gcggagaaga ccgcaaaatg gtaatttaac ttcaatttcc cttctttttt acaggatctt
    20341 cttagggaat tttgcttgga aaagtggtca aaagggaaaa gactggcatt ttatttattg
    20401 attttcagga aaatcccaat attttatttt cattcatctt tataagggac attacatatg
    20461 taaaaagaaa aaacattttt agatcagcct cgtgggctgt cgactttatt cttcgcttta
    20521 tgtttagcta gaaaaccgat ccgaaacatt cattgatcga ataataaaaa cttgattttc
    20581 cacctgtact ttgcctgaat cgtgaaagca caggtatgaa aatcaagttt tttgttgcga
    20641 aaaaacgtcc tagtcaatca gttggccgag cggctggcgg ctgatctgct cctgatcaat
    20701 aacgagtgct tcttggaagt gttgctgcaa aaagtagcgg tcatggctga ctgctaggag
    20761 cgttccttga tagtcttgca gcatttcttc aatttcttct cgagcatagc tgtcgaggtg
    20821 attggttggt tcatccaaaa tcaggacgtt gattttttct aaaaagagtt tcagcaacgc
    20881 caaacggatt ttttcaccac cggataaatc tttgatccgt ttacccacat cgctttgata
    20941 aaagccactt ttggctaatt gccgtcgagc gacttcttcg tttccagtca atgtgatggc
    21001 gtaagccagc aaacggctat cgggatcggc aaactgcagc tgctgcggca aataaccgat
    21061 ttgcaagcta ctacctaatt tgatggtgcc accgtaaggc ttttcctcac cgagtaataa
    21121 tttgagtaag gttgattttc ctgcaccatt gtcgccgatg atggctaaat gatctccacg
    21181 atagactgca aaactgctgt ctttgaatag tacttgatca gcgtactgct ggtgcaagtt
    21241 ttgaatgatg aaaacttctt tgccagattt tccaccatta gacaagcttt gcagccgatt
    21301 tttcggggga ggtggcggtt tgacgagtgt cagtttcgcc aggcggcgtt ccagttcttt
    21361 ggcttttttg aagaaggact cattgtctcc ttcatgtgcc cactgacgaa aacgacgaat
    21421 catgactttc aaccgctgga cttctttttg ctgcagttcg taattttttg tcagctcagc
    21481 caagcgagct tctttcaaga ctcgaaaacg gctgtagttt ccgggatact caatcaatgc
    21541 accttcttcc agttcccatg tttttgtggt gacttgatcg agaaattgcc gatcatgaga
    21601 aacgaccaga accgattttt ttgtattctg taaatagttt tccaaccagc gaatactgtt
    21661 aagatctaaa tgattggtcg gttcatcgag taaaagtaaa tcggcatcgg ctgctaaaat
    21721 tttggctaaa gcgactttaa ctcgttcgcc tccgctaagt tctgttaacc gtgcttcctg
    21781 acagtgtgga atccctaagc cttgcaagat cgctgcaatc cggtcttcta atacgtaacc
    21841 gcctagttct tcaaagcgat gctgcagctc accgtaacgg gccagacact tctctaagtt
    21901 tgtatctggt tctgtcatca atgcttcgta gcgactcatt tgctgtttga ggtcctgtat
    21961 ctcagcggca gaatggagca agtagtggtt gacggtcgct tcggaaactg taagttcttg
    22021 ttcgaccgtc ccgatcttta agcctttttt gcgactgata acaccgctgt cgacaccttc
    22081 tctgccggtc aagatttgta aaaaggttga tttgccagtt ccgttgatat cgactaaggc
    22141 aattttttca ccagcttcaa tttgacagga taaatcctca aataatggat tcccgccgat
    22201 tgttttttta atagaaattg cttgtatgat catgatctct ctccttgaga ccactcaaaa
    22261 aggccatggt tatccctggc ctgtgttatt taaactcaga ttatcatgag aaaaatacct
    22321 tgagatggcc attgatttta cgtgaattca actgttttgg acaaacttgt cccgtcaaca
    22381 gcaactgtaa aaccaattgc atggctaaat gcgatatggt atcgttggta attttcatta
    22441 cttgttattt tagtcatgcg catcatagta ttttccctcc ttcgtgtagt tgtcttttcc
    22501 agtgtacctg aaagtgtcag aaagttcaac tagcgctgac atttacttaa tatttatcga
    22561 tgtgtaattc tgtcagagag ctttttttat ctgactgagg gtaatgtctc gtggaccaca
    22621 tcattgtatt ctgagctaaa ccagcgttca ggcgaaatca tatggtgttt gataaaaaac
    22681 tttgtctaca tgacttcctt ttgccatttg cttaataccg ttgcattaaa cagctcttct
    22741 ctttggaaat ctcaccaaca tcgcgttcag gagtatcgtt gtggttgcaa tggcaagcgg
    22801 tagcctttca tcaattcttg aatgatttac cgaatgtagc acttgccttc tcgcggtacc
    22861 cgaacccgaa ataaaatttt agtttggcga taaaatcggt atccgccatc gatacttcat
    22921 attttttcgt atcagattca tcggaaactt ccacattgac gaaccaataa gctttggcat
    22981 gcttaggtcg ttttaaacca caccagggcg actaatcgcc ctggtgttca ctttttttat
    23041 tttacttgaa ctcattgact gttttccctt tattttaaac attataaaga ccgatgagtg
    23101 cttgccgcgt cttttgtttt tatcacggtt ttttgtgcaa aaaaacctgc cgatagttgg
    23161 caggtcgtct acgtgttaag ctttggtgtg gtctttagct ggcaattggt tttctaaatc
    23221 agttcggaca acaagcacat cgcaagctgc gtttctaata acatattcag aaacagaacc
    23281 aataaataaa cgttctacag cattcaatcc tgtggctcct aacataatca aatctacttg
    23341 attatcttct gggatttctc tggcaataat ttgttttggt gagccatatt ctacgacaga
    23401 ggttacatta ttcaagccag ctttcttcgc attgctttca taatctgcca atgtttgctt
    23461 tgccatttca gtagcttgtt ctgcaagcat tccatcaaag gatgaaacgg tttggaaaga
    23521 acgtgtgtca atgacatgtg ctaatagaag ttctccgtta tttcggatag ctacattgac
    23581 cgccttttta aaagctaatt cagcttcatc agaaccatct acggcaacca tgattttttt
    23641 gtattgttgt aacataagca ttcactcctc aagtcaatcg tattgtactc ttttattgta
    23701 gaacacttta gggaaaaaga gaattattta ctgctttggt tcagcaattt tttttgcaaa
    23761 tcggatactg aaggtggcag aaacaataat tacgatcagt aaaaagacca aggcaataaa
    23821 gcgttgattg aaaaaagctg ttaaaatacc cattacgcct aaaacagcat atgcagcacc
    23881 gtaaaattgt aggaatcctt gatttttatc atttttgttg atcaatacaa aaaagatatc
    23941 ctgtttatta aataaataat agctaacgaa taataatact aaagtggcga taatcattaa
    24001 aatcctaatc atgaacaaat cctccaaaat aagcaacaaa tagaaaaagc aatttgccaa
    24061 gagcaaattg ctttattctt taaaagatgg taatctccgt taatgccgtt gtttgtcctg
    24121 tagtattgat cccgcgaatt caagcaggtg ggtcccatgg tcgtagcttc gaactaccaa
    24181 atgataaggc atgttaccag cagtgacaca agacgggtcc caagatatca ataaaaatgt
    24241 tcggtcaaca cacaaagagg acaattcgaa catcacagat ttcccacctc tatagtagca
    24301 tatgaattta aaaagagcaa tgagagagct cgcagaaaca gaaccgtttt cttgtctagt
    24361 tttttggagg atgctttttc cactctttta tacggtaata ctgttgaccc agagcctgtt
    24421 catatttccc tgtatctttt ggctgataat actgagcatt cttcagttta tctggtaaat
    24481 attgctgatt gacccaagcc tgatcaaaat gatgaggata ctgataaccc acgccacggt
    24541 tgagtgattt cgcacctttg taatggctat ctctcaaatg atcaggaaca tctccagctt
    24601 tgccctctcg aatatcagca agtgctgcat ctaatgccat gtaggcagaa ttggattttg
    24661 gcgacaagca aagatcgacg acgacgtctg ctagtgggat acgtgcctca ggcaatccca
    24721 atttttcagc agctaataca gcgttcactg tacgagccgc agcagcagga ttacctaaac
    24781 ctatatcttc ataaccgata accatcagac gccggcaaat acttgccaga tcacctgcct
    24841 caactaaacg ggcgagataa tgaagtgcag catccacatc acttccacga atcgattttt
    24901 gaaaagccga aataacgtca taatgagcat ctccattttt gtcatgggta agagcttttc
    24961 tttggacaca ttcttcaatg atcgacagtg taagatgaat ccttccttct ttatctgaag
    25021 gagtcgatag tgtagccagt tccagcccat tcaatgcact ccgcaaatcg ccgttcgttg
    25081 cacgcgaaag gtgaagaaga gcatcttcgt ctaactggat tgcttgttgt ccaaggccgc
    25141 gttctttatc ccttaaagca tgttcgactg ccaactggat gtcttgttct gttaatggtt
    25201 tgacttcaaa gatctgtgta cggctgcgga tcgctggatt gatggtaata taaggattct
    25261 cagtagtcgc tccgatcaag atgattcggc cactttctaa atgaggaagc agaaaatcct
    25321 gttttgtttt atctaatcgg tgtacttcat ccaataagag gatcactgta ccactcattt
    25381 ttgcttcttc cgctacaact tgtagatcct tttttgaatc tgtagcggcg tttagcatgc
    25441 gaaatgcata attagtagag ccggcgatcg cacttgcgat actcgttttt ccagtaccag
    25501 gaggtccata tagaatcatg gaagagagca tgcgagcatc gaccatgcgg cgtatgattt
    25561 ttccttcccc gactagatgc tgctgaccaa cgacttcatc gatcgttcga ggtctcatgc
    25621 gataagccaa tggctgttgc ataggatttt cctccttctt gtttttacgt cccttaatta
    25681 tatcatattt agcaagatgc ctagaaagaa cagccttctg ttgttttgtt ttcatagctt
    25741 atccaactaa tattgtgtat gataaagtag gtgagaaaaa tgacggataa acaaaagata
    25801 gatgaatttt tttggaataa aacgacgcca gaagtggcac gggatttatt ggggatgtat
    25861 ttggagcatg acacaccaga aggaagatta gctggatata ttgtagacgc ggaagcatac
    25921 ctaggtccag aagacgaggc cgcccatagc tatggtttaa gaagaacacc gcgtgtgcgg
    25981 gcaatgtatg aaaaaccagg tacgatctac ctttacacga tgcatactca tcggattttg
    26041 aacatcatta ctcagccaga agggattcct caaggagtaa tgatacgtgc aatcgaaccc
    26101 gccgcaatga ttgatcaaat gagtaaaaat cgtggcggaa aaactggtcc agatatcagc
    26161 aacggtccgg gaaaattagt ggaagcatta gcaattcctc aagaacttta tggtcagtct
    26221 atagcagata gttcgttacg cttagttttt gagaaaaaaa agacaccaaa aaagatcaat
    26281 gctttgccta gaatcggtat cccaaacaaa ggtgtatgga cagaaaagcc gttgcgtttt
    26341 gttgtgtcgg gaaatcctta tatctcttta caaagaaaaa accaaatcga gaaagattgg
    26401 ggttggagga aagaaaatga aaaagaagga tctattaaca tatttagatg aaataattga
    26461 aaaacaagtg acagactatg atgtagcttt agattgggat acaaaaaatc atacgatcga
    26521 gatcgttgtt cgcttgtttg cagaaaataa agcacaattt gagattgatg atgcagaagg
    26581 gatcgtttcg caagaaccaa ttatcgaatt tgaagacggc gtacttcttt ttaatcctca
    26641 gaaatctgtt tttgatgaac gcaattatct agcagtaatc ccttatgaag gaaaaaaagg
    26701 gttggcaaaa tcagtagctg attctttagt ggaatatcta aatgaagtat tagctcaagg
    26761 gcaaagcgat ttgcttgatt ttttggatga agacgatgac gaagctgtat tcgagcttca
    26821 ctgggataat caacaactag cagaattagt agaagtaaaa cagcaaaatg aagcagatac
    26881 ttatttaccg tacccaagtt attaaggagt gacagatatg aaatggaatg aagtaaaagt
    26941 agaaactgaa agtgaagcag tcgaagcagt tgctaatatt ttgatggagg caggggcgag
    27001 tggtgtcgcg attgaagatt ctttagatgt tgaacatttt cagtcggatc aatatggtga
    27061 gattttagat aaaaatgatt ttacatcgct gaaagaaggc gcactagtca tggcttattt
    27121 tccggaaacg actttcttac cggaaatctt gccttttatc aaagatcgta tcacacaatt
    27181 acctgagttt ggtctagcta ttggaaaaaa taaagtaaca gttagtgaag tagaggaaag
    27241 caactgggca acagcctgga agaaatacta tcatccggtg cgtattacgc gaatgctgac
    27301 ggttgcgcca agctgggaaa actaccagac agaagatcct ctagaaaaaa ttatcacact
    27361 agatcctggt atggcatttg gcacaggcac tcatcccacg acttccttga cattgacagc
    27421 actagaaaca gtcttgcgtg gtggtgaaac attgctagat gtgggaactg ggtcgggcgt
    27481 gctaagtatt gcagcaaaat atcttggtgc aaaagatgtc tatgcctttg atttagacga
    27541 agtagccgtt cgttcggcga aagaaaatat ggatatgaat gaaatagcca aagatgtcca
    27601 tgtatcagct aacgatcttt tgacaggaat cgagattgaa agtgatgtaa tcgttgcgaa
    27661 cattttagca gatatcatct tattgatgat cccagatgct tggcgtttac tgaagcaaac
    27721 aggcacgctg atcgtttcag gaatcattga agagaagaag caaatggttc tggatgccat
    27781 gttagaacaa gggtttatcg tggatcaaat tttccagcaa aaagactggt atgcaattat
    27841 tttgaaaaaa ccagaggtgg aatagatgca gcggtatttt ttaaaagaat catatgaatc
    27901 aaaagactat tttgttttag aaggggaaaa ctatcaccac attgtacggg ttatgaggat
    27961 ggaacccgag gatgaagttt ttttggcatt caatgatcaa atagccattg ttgcagaaat
    28021 cacggagatt actgaagagg ctgtttatct gaaagaaatt aaaaaagaag aaaatcaaaa
    28081 ggaactgcca agttcgatta ccatagcgag tggctatcct aaaggggaaa agctggattg
    28141 gatcgttcag aaatccactg aattaggagc gcaagctttt cttggtttcc cggcatcagc
    28201 ctctgtcgtt aaatgggatg caaaaaaacg tatgaaacga caacaacggc ttgaaaagat
    28261 tgctcaagag gcagcggaac aatcccatcg gcagatcgtg ccatcagtag agttattgga
    28321 aacgaaacaa gatttatttg agcggctaga tgattatgac gtggtcttgg ttgcttatga
    28381 ggaatcagca aagcaaggcg aaaaatcaaa tgcagccaaa attcttagtg aaacaaaacc
    28441 agatagtcga atcttagctg tctttggacc agaaggtgga ttatcccgcg atgaagtcga
    28501 ttattttgta gaacacaaag ggatactttg tggattagga ccaagaatcc ttcgtacaga
    28561 aacagcacct ctttatttat taagtgctgc tagctatcaa tgggaattaa tgaatccttc
    28621 tttcagctaa cgttcttcat taataaaaga gtatcgacag aaagaattga aaaaatagtc
    28681 aacaatttgt ataatagaaa caattattta tacaatggat acgaaaaatc gtttgaaaaa
    28741 gcttttaaaa agcgaacatt tccgtaaaaa aacgtataac tgcacggatt ttgggacaat
    28801 tatcaaacag atatttgtcc cgattcccgt tttaccgaaa aaagaaaggg ggatcgattc
    28861 ctgtgacaaa agaagaaatt ttaacgggac ccggtgtcat aaagctcgtt agtcaataca
    28921 tgggtcccga acatgtagca tttgttgaga aagcatgcga atacgcgact gctgcacatg
    28981 acggacagtt tagaaaatca ggcgaaccct acattattca tcctatccaa gtcgcaggta
    29041 tattagcaga tttaaaaatg gatccccata cagtggctac aggcttctta catgatgttg
    29101 ttgaagatac agaaatcact ttagaagatc tgagagaaga atttggcgat gacgttgcta
    29161 tgttagtgga cggcgtaacc aaattaggga aaataaaata taaatcccac gaagagcagc
    29221 tagcagaaaa ccaccgaaag atgctgctag caatggctca agatttacga gttatcatgg
    29281 tcaaattagc tgatagacta cataacatgc gtacgttgaa gcacctgcga gaagataagc
    29341 aaagaaggat cgctcaggaa actttagaaa tctatgcgcc tcttgcacat cgtctaggga
    29401 tcagccggat caaatgggaa ttagaagata cagcacttcg ttatctaaat ccaaaacagt
    29461 attaccgtat cgtccacttg atgcagacga agagagaaga acgtgaaaaa tacgtaagtg
    29521 gtactgttga agatattcga atagcgacag aagagctggg gatttttgca gaaatctatg
    29581 gacggccaaa acacatttat tcaatctatc gtaaaatgaa agatcagaaa aagcagttca
    29641 acgaaattta tgacttgcta gctattcgag tgatcgtaga ttcaatcaag gattgttatg
    29701 ctgtattagg agcaatccac acaaaatgga aaccaatgcc cggcagattt aaggattata
    29761 ttgccatgcc aaaagctaat atgtaccaat ctttgcatac taccgtgatc ggtccggcag
    29821 gaaatccggt agaaattcaa atccgaacac aggaaatgca tgaaatcgct gaattcgggg
    29881 ttgctgcaca ctgggcctat aaagaaggaa aaaatgaaaa agtagaacca gatggtatga
    29941 cgaaacaatt aagctggttc catgagatac tcgaacttca agacgaaagc tatgatgctt
    30001 ctgaatttat ggaaggcgta aaaggagata tctttagtga taaagtctac gtcttcacac
    30061 caaaaggaga cgttactgag ttaccaaaag gatccggacc attggacttt gcatacagta
    30121 tccatacaga tatcggtaac aaaaccactg gtgcaaaagt aaatggcaaa atggtgcagc
    30181 ttgattacaa attgaaaaac ggagatatca ttgagatcat gacttctcca aattcatttg
    30241 gcccaagtcg cgactggttg aaattagttg ctactagcaa agcaagaaat aagatcaaac
    30301 gtttcttcaa agcccaagat cgagaagaaa atgtgatcaa aggccacgaa tccgtggtca
    30361 aatgtattac agatctagga tttacgccta aagatatttt gacgaagaac aaactgcaag
    30421 aagcactcga tcgttttaat tatcaaacag aagatgatct ctatgcagct gtagggtatg
    30481 gagaagttag ccccttgacg atggccaatc gtctgactga aaaagaacgt aaagaacaaa
    30541 aaatcgagca gcaaaagcaa gaagcagaag aaatcatgaa tcagccgaaa aaagaacctg
    30601 acaaaatgaa agtacgtcat gaaggtggcg ttgtcattca aggggtagaa aacttactaa
    30661 ttcgtatcag tcgctgctgt aatccgattc ctggtgatga tatcgttggt tatatcacta
    30721 aaggcagagg gatatccatt catcgtcgag attgtccgaa tgttcagcct gacaaaccaa
    30781 atgtagcaga acgtttgatt gaagtcgaat gggaagatac atcgaataca cgaaaagagt
    30841 atgatgcaga tttggaaatt tacggctata atcgttcagg cttattgaat gatgtacttc
    30901 aaacagtcaa tgcgctaacg aaaaatctca acagcgttga agcacggacg aataaagata
    30961 aaatggcgac gatccatttg acggttggta tccagaattt atcccaccta aagagtatcg
    31021 tggataaaat caaagcagta cctgatgtct acagtgtacg ccggacgaat ggataggagg
    31081 acagatatga gagcagttgt tcaacgggta agtaaagctg ctgtgtcagt cgatcagcaa
    31141 gaaattggaa aaattggcta cggcctactt gttttattag gcattcatga aaccgatacg
    31201 caaaaagatg tggactatct ggtaaaaaaa attgcacaaa tgcgtatttt tgaagatgaa
    31261 caaggaaaaa tgaatattag tgtagaagat gtgggaggac aaattttaag tatctctcaa
    31321 ttcacactgt ttgcggatac gaaaaaagga aatcgaccaa gctttgtgga agccgcacga
    31381 ccagaaacag ctattccttt atacgaatca ttcaatgaag gaatcagaag tcgcggcatc
    31441 acagtcgcta ccggacaatt cggagcagat atgtcggttt ctctcgtcaa tgatggacct
    31501 gtaacgatca ttattgattc taaaaacaaa taaagtaatc tggttttcac tagtaaaaac
    31561 tatgaaacac gtcgcttaag ttagactgat aaactctaac ttaagtggct tttttgtctg
    31621 caaaaagcgc ttcttttgtc agcagtcgga aaaaaagtta agcttaaatt atacaagtta
    31681 ttttttatag gaggttgctg aagatgaaag aagtaccagt agtagatatc aaaatcacag
    31741 atttaaaaaa attgtatcaa aaaggataca acatgttgga ggagttacgc catgaagcgg
    31801 acgctcccgt cgtcaaagca aagattttca ataaagaagc tatcacgatc tacggatcat
    31861 cagcagcaaa agtattttat gatccaagga atttcaaacg aaaaggtgct atgccaaaat
    31921 tagtattgaa gacacttttt ggtcaaggtg gagtacagac tttggatgga gctgctcatc
    31981 accatcggaa aaatattttt atggatctaa tgacaccgga aagaatggaa gattatcatc
    32041 gtatcttaga taagaatctg acacaagcac ttgaagcaca gcatggtcaa tttgaactat
    32101 ttgatttaag taagatggtt ttctttacat ctatttgcga gtgggcaggt atcaatttat
    32161 cagcgatttc taaagatgag gtcgaaaaat tagcagaata tcaaatctct atgatcagcg
    32221 gaacgtttac ttctccgatt gatcacataa aaggtgtcga gaatcgcaaa aaatcagaaa
    32281 aatgggcgca aggcttgatt gaagaagctc gacaaaatcc tgtggctgga aaagaaaatg
    32341 tggcattata tgcttttgcc aatgccacag atttagatgg tcagctttta cctctggaag
    32401 tggcagctgt tgaattattg aacatcatcc gtccaacggt tgctctaact gtgtgggcgg
    32461 cattgatggg acatgcactt ttcagtagac cagatttgta tcagcaatta aaaaatgact
    32521 tttcgacgtt acaagatccg tttattcaag aaatgagacg ctattatcct ttcttcccaa
    32581 tgctacctgc gatttcgctg aaagaagtag aagtagatgg ttatcgtata ccagaaggca
    32641 gttgggttat tttagattta tatggaacag accatgatga acgaacagtt gaagcgccag
    32701 attcctttat gatcaaacgg tatgtaggga aagcaaaaga tatttcctat aaagaagaat
    32761 atgagatgat tgcacaaggc gggggaaatt ttcgacaaat gcatcgctgt gcaggagaat
    32821 ggatcactct tcatagtcta cgtgtgtttt ccgatcagct agtaaataag tttgagttct
    32881 ctgtacctga acaagattgg acgattccat tcaatcagtt tccgacttat ccgaatagcc
    32941 gagcgctact ttacaagaat tagattagca taaacttatc gaaaaaaagg aagatcaaac
    33001 agtgctgatc ttcctttttt catattacta taaattgata aggtcaattg atcaaaatcc
    33061 acgaaatgct tcatcaatgg tttgataatc ttcattggat aaatgcaaat ctaaagcttg
    33121 gacattgtca gatacttgct ctggacgctt agctccaggt ataactacag acactcgtgg
    33181 attcttcatg taccaagcta aaacaagttg cgcaggagtg gcttggtatc ttttagcaat
    33241 ggggcgaagt acatcgacag cggttaaaat ttctctgaaa cgttctcctt ggaaatctgg
    33301 atttcttttt cgaggatctc cttcaccaaa ttgtttttcc tcaccaagtt catattttcc
    33361 tgtcagcaaa ccggaagcaa gaggaaaata gggaacaaaa ctgattttgt ttttttcaag
    33421 ataagggaat aattcttttt cagcctgccg atggatcaaa ctgtatttat cttctactac
    33481 atcgacatat ccatctgcat tcgcttcttt caattgttct aacgtgaagt ttgacacacc
    33541 gactgctcgg atttttcctg cttcttttaa ttcgtgcaat gtagcaactg attcattttt
    33601 tggtgtgctt tcatccggaa agtggatata aaaaatgtcg atgtaatcag tttgcagacg
    33661 ttttagtgca tcttccaccg attgtttgag aaactcagga gaattatcaa atgttcgccc
    33721 tttatttggt acatgagcag cttttgtagc gatgacgaca cgtgagcgat catattcttt
    33781 ttccttcagt acctcgccaa tcagttcttc ggaacggccg tttccgtaag cataagcggt
    33841 atcgatcaaa ttgatcccgc tattcaaagc tgtacgaact acttgttttc ctgtctcatc
    33901 ttccaaaccg tcaaaaagat tatgtcctcc aacggcattt gctcctaatc caagctgttc
    33961 tgcgtagact tgtgaatgtc cgattcttat ttctgccata gctgattcct cctttttgtc
    34021 tagtctaaaa caaaatacca aaaagctgaa ctaaaacgtt tttgtgaggg gaaatagtca
    34081 gatatctctt gactttgaga ctggttttta gtaggttata agtaatatcg aatgtcaatg
    34141 aaagagcgag taagtttttg aaactttaca agagaggatc atcgaggctg aaaatgatct
    34201 aaagtttcat tagcctaaag acactctgga gatggacgag tgaaaagccg tcccgcgtac
    34261 gagcgttatt cgtaagagga aagagaaatc tttcaaaatt aggtggtacc gtgtatgaat
    34321 ttcattcacc cttgttcatg atgaacaagg gtgatttttt tatttacata gctaagaaag
    34381 ggagagaaaa ctatgagttt ccaaagacca aaaggaacaa atgatatttt gccaggcgaa
    34441 tcagaaaaat ggcagtttgt cgaagaaaca gcacgtttat tattcagaga ttatcagtat
    34501 aatgaaatgc gtacaccgat ttttgaacac tatgaagtga tatcccgaag tgtaggggat
    34561 acaacggata tcgtttcaaa agagatgtat gatttttatg acaaagggga acgccatgtg
    34621 acgcttcgac cagaaggtac agcaccaatc gttcgttctt ttgttgagca taaattgttc
    34681 ggcccagagt atgcgaaacc gttcaaaact ttttatatgg gaccaatgtt tcgctacgaa
    34741 cgccctcaaa aaggacggtt acgtcaattc catcaaatcg gagtagaagc atttggatca
    34801 gaaaaccctg caactgatgt ggagagcatg gtgatggcac ttgatttctt caaacagttg
    34861 gggatcacgc aaatccggct agtcatcaat tcattaggtg ataaagaaac gcgtcaagcc
    34921 tatcgtcaag cattgatcga ctatctttta ccttttgaat cagagctgag tgaagactct
    34981 aaacgccgcc ttcatgaaaa tccattacgc gtattggaca gtaaagacaa acgagatcaa
    35041 aaatttgtgg cagaagctcc atctatctta gattatctaa gcgagcctgc tcaaaaacat
    35101 tttgataccg ttgtttccat gctggatgca ttggctgttc cttatgaaat cgacagtaat
    35161 atggttcgcg gactagatta ttacacacat acgatttttg aaatcatgag tgacgcacca
    35221 aaaatggggg cacaagcaac gatttgcgca ggcggacgtt atgacaatct agtggaagaa
    35281 ctaggtggtc cttcaacacc tggcttcgga tttgcaatgg gtatcgagcg attactcttg
    35341 actatggagg cagaggaagt agttatccct gcatttaatg agttggatgc ttacgtggtc
    35401 gcactcggag acgaaacaaa tatcgaagca ttgaaagtgg tccaagcgat tcgaaacttt
    35461 ggtttttcag ctgaccgcga cttcatgaac cgtaaagcaa aagcgcaatt taagacagca
    35521 gataaagcga atgccaaatt agtattggtg atcggtgaag atgagcttgc tagtcaaacg
    35581 attaatgtca aagcaatggc aagccgaaaa gaaaaaagtt ttccattaga agagatatat
    35641 gaacattttg atgaagtcta tgatgaaatg actttattcg agtaggagga aaaaaataaa
    35701 tggcacaaag aacgatctat tgcggattag tttccgaaca acaattagaa caaacagtta
    35761 cattaaaagg atgggtacaa aaaagacgag accttggggg agtaatcttt atcgacctac
    35821 gtgatcgtga aggaatcgtt caaatcgtct ttaatcctga aatcaacaaa gaagcatggg
    35881 aaatcgcgga taaatgtcgc agtgaatacg tgatcgaagt gactggggaa gttagaagac
    35941 gcggagaaaa tgcagtaaat cctaaaatgg ccactggtga attcgaagtg attgcaagtg
    36001 aaatcgtgat tttgaatcaa gcaaaaaccc cgccatttca aattgaagat gatcaagcaa
    36061 tcagtgacga aatacgtttg aaatatcgtt atttggatct gcgtcgtcca aaaatgacta
    36121 aaaacatcaa attaagaaat gcgacaacaa aagcgattcg tcattattta gatgataatg
    36181 actttctcga tatcgaaaca ccttacctag ggaaatctac accagaaggc gcaagagact
    36241 acctagttcc ttcacgtgtc catgctggac atttctatgc gttgccgcaa tctcctcaat
    36301 tgtttaaaca attgctgatg aatgctggat tcgaccgtta ttatcaaatc gttcgttgtt
    36361 tccgcgatga agatttacgt ggagaccgtc agcctgaatt tacgcaagta gatattgaaa
    36421 caacgttctt aacagcagaa gaaatccaaa cttacacaga gggattgatt gcgaaggtaa
    36481 tgaaagaagt tcgcggtatc gaagtcacat tgccatttcc tcgtatgacc tatgatgaag
    36541 caatggctcg ctacggcagc gacaaaccgg atacacgttt tgatatggaa ttgatcgatc
    36601 taagcgatac agtcaaagaa gtagaattca aagtcttcca aatggctctt gaaaacggcg
    36661 gtgtcgtgaa agcattgaat gctaaaggcg ctgcagatcg ctactcacga aaagacatgg
    36721 atcagttagg tcaatatgta ggacaattcg gagctaaggg gcttgcatgg ctgaaagtag
    36781 aagaagacgg attgaaaggt ccaattgcta aattcatggg agaagcaaca gaagcaatca
    36841 ttaaagcaac agatgcaaaa ccaggtgact tgctgatgtt tggcgcagat aaaagtgaaa
    36901 tcgtcgctgc tgcattaggt gctatccgga cacgtttagg aaaagaatta ggactaattg
    36961 atgaatcgaa attcaatttc ttatgggtaa ctgattggcc acaatttgaa ttttcagaag
    37021 aagaaggtcg atatgtctct gctcaccatc cattcacgat gccaaaagaa gaagatatcc
    37081 cgttattggc tgaagaccct gcaaaagtct atgcagaagc ttatgatatc gtcttgaacg
    37141 gttatgaatt aggcggaggt tctatccgta ttcatactcg tgagcttcaa gaaaaagtac
    37201 tagaaactct aggctttaca aaagaacaaa tggaagaaca atttggtttc ttgttaacag
    37261 ctttagatta cggattccct ccacatggcg gtattgcatt aggattggac cgttttgcaa
    37321 tgttattagc cggagaagat aatattcgtg aagtcattgc ctttcctaaa aacggaaaag
    37381 cagcagatgt gatgagtgat gcaccaagta tggtttcgcc tttacaattg ttcgaattga
    37441 atattgatgt aacgaatata gaagaatagc cgataaaaag gtaaaaagga tttgtcgaat
    37501 acgcttcttt tgacaacctt gatttaattg aaaaacactt tataatgaag ccataacata
    37561 taaagcgggg cttttccatg aaaaaaacat tcgaaaattg ggcttatcat gattacacga
    37621 ccaagctttc ggtatctatc gtttatgcgt tgatcgcatc agtcgcattg aatttctttt
    37681 atcagccagg acatatttat tcaagtggta ttactggtct agctcagatt ttgacgactt
    37741 tgtcaaaatc actgatagga ttcgaagtac cggtatcaac aactttgtat ttactgaata
    37801 tacctttgtt tgtattggcg tggttaaaaa tcgggaagaa gtttacaatt ttcactttta
    37861 tcacagtcac tttgacctcc ctttgtatcc atattttacc ggaaacagtc ctatctaccg
    37921 atccaatcat ctgtgcgttg tttggggggg ctgtgatggg actaggtatt gggctagcgt
    37981 tgagaaacgg cctatcatcg ggcggtctag atatcgtgag tatcacgatt cgtaaaaaaa
    38041 caggaaagtc gattggttct atttcgatct acttcaatgc attgatcgtt tttgttgccg
    38101 gaattctatt tggctggcaa tatatgtttt acagtacgtt gtctattttc gtcagtggaa
    38161 aagtaactga tgtcgtttat actaaacaaa agaagatgca ggtaatgatc gtaacgaaac
    38221 atccagaaaa agtagtaaat gaaatccaaa actgtatgcg tagagggatc acgatcatca
    38281 atgaagcaga aggtgcttat aagcatgata agcagacagt attgatgacg atcgttacac
    38341 gatacgaact gcctctactt agagaagcaa tgaaagcttc tgatccacaa gcttttgtaa
    38401 gtattgcaga aaacgtccag atacttggtc gtttctatga ggatggcatt tagatcgagt
    38461 ttgacaaaaa ttcatgaaat actgctgtag acagacgttc gtttgtctgc agtttttttc
    38521 taccatagta gaatcagcaa agaaaaactg cttttttatt tgaaattttc ttaaagttga
    38581 aaaaaagcat gtttattttt ttaaatttat gggatgatag aaaaaaatag tagagaaagg
    38641 acacgattct acttgctatt ttcacttaat acccgtatat tacgaatgaa aaaagtttat
    38701 gaaaagttgg aatattgaat ggaaaaacaa aaaaggttaa aaaacagccg ctacatcact
    38761 gggtttgatg ggctccgttc attagctgtg gtgggtgtta tcttctatca ccttctgcca
    38821 actagtatga aaggcggcta tttgggcgtt ccgatttttt tcgtcgtatc tggttatttg
    38881 atcacggatc tgctgcggca ggaatgggaa caaaatggaa aaatcaatat ttggcaattt
    38941 tatgtcagaa gaatgaagag actctatccg gcacttgttt ttatgcttgt gacagcttcg
    39001 gcttatatca ctttgtttca gcgagggctg ttaaacaatc ttcgaggagt tgtggtcagc
    39061 agcttactgt atgtcaacaa ctggtggcaa atcaataacg gtttatctta tttcgatcga
    39121 tttgctaatg agtcaccgtt tacgcatatc tggtctttag cagtagaagg tcaaaactat
    39181 ttggtttggc cgatcttgtt tgtattgttg atggtatttg ttcgtaaaag aaaatggatc
    39241 ttttatactg tgttaggtac atccttcctc tccgccatct tgatgatgat cttgtataca
    39301 ccaggaggcg atccaacccg tgtttattac ggtaccgaca ctcgtctgtt ttctatatgg
    39361 ttgggaagtg cgcttgcgtt tgtttggcca agtacgcgtt taaagaaaaa catcccgaaa
    39421 caagcgaaaa atattctgaa tatcgctgga ggaatctcgc ttttggcact gattctttgc
    39481 ttcttctttt tagatgatca ttacaatttc atctattacg gaggaatgct cctagtgagt
    39541 ctgttatgcg tagtgctagt tgcagtgaca gcacaccccg gagcaagttt gaatcgatgg
    39601 ctgacaaatc cagtatttac ttggattggc aaacgaagct atggcattta tctgtatcag
    39661 tttccagtta tgatttttta tgaagcaaag gtgactgata ttgccgatca tgttttgttg
    39721 catactttag tggaaatcac attgattcta atcatcagtg aactttctta tcgatatgtg
    39781 gagcgtccgc tagcacgttt tgattatggt cgtacattcc aagtgatgaa aggatggttc
    39841 agcaaaccga tcatcagtaa gagcaaacca tgggttgttc caggcacatt acttgtagca
    39901 attgcactcg ttggtttcgt tacggcaccg aaaaacgctg taacagccga tcaaaaacaa
    39961 ctgcaggcaa aaatcgctga aagtaaaaaa ttagcagaag aaaccagaaa caattcagaa
    40021 aacaccacta gttcggttga ccaagcgatc cttgataagt acgagttgtc caaggcacaa
    40081 gggaataagg cacaaaaatt agaactaacg gcatttggtg attcagttat gttggatgct
    40141 gcaacggatc tacaagaact ttatccaaaa gtagtggtag atggagatgt cggacgacaa
    40201 ttatatgcca gtgtaccgta cattgaaaaa ttaaaggaac aagatttgtt gaaagacact
    40261 gttctgattg gactaggaac gaatggctca ttctcggaag ctcagtttga tagtgtgatg
    40321 gatgcaatcg gtgatcgaaa agtatactgg gtcaatgtcc gagtaccgac gaagcgctgg
    40381 caaaatgacg tgaatgcgat gcttgaacag atgacgaaaa aatacgacaa tatgacgctg
    40441 attgattggt acagctacag taatgaccat agtgactggt tttatgatga tcaagtccat
    40501 ccaaatcctg aagggatgac acattacatt cacttagtca gcgaagcctt gctaggagga
    40561 agttcaagta cgaacagcag tgagacaaac gtgacagaat gatagctaca aaaaacttca
    40621 ggcaaaaaca tttgtttttg cctgaagttt ttttatattt accgtgtttt taacattcat
    40681 atagattcag gaagcaaaac ggttgagtgt gtctgtaaat aacgttcttt ttgttttcgg
    40741 gtgagttttt tgaatgcagc ttctgctttt gttgcttcgc ttcttgaaga aaatacttct
    40801 gcatggatca tcttaacagg tcttcttttt tgaagacgag tatatttagc accagtacca
    40861 ttgttatgtt cctctaatcg tctagaaaga tcggttgtat atccaccata aaatgtctga
    40921 tctttacaga ataatacgta aaaataatgt tcatttgcca tagagcatct cacgaatctc
    40981 tgcagtgtat tctccatcca tattgtaagt gatcaatggt ggagcgactt tgaatccgtc
    41041 taatttgcct tctttgattc cttcaactaa caaaatattt gcctcttttc caggttttgg
    41101 gtaaacaaat tggatccttt taggtgcaat tcgatagtct tccatgacct gaatgatttc
    41161 taagaaccga tcaggacgat ggaccatagt aaagcgtcca tttgttttta gtagcttact
    41221 tgaaatatcg atcacttctt ctaacgtcgt atgaatctca tgacgggcga ttgccaggtg
    41281 tttattaggg ttcgttttat ttgttggcag ccctttgaaa taaggtggat tacagacgac
    41341 taagtcacaa gaattatgtc gaacgacaga taagctgtct ttcagatcga tggtgtggac
    41401 agtcatttgt tcatttaaat ggttaagcgc gatactacgt cttgccatgt ctgctagacg
    41461 aggttgaagt tcgatagcgt cgatagccgc ctgggttcgt ttacttaaaa aaagtccaac
    41521 tgcgccattt cctgcacaaa ggtcaacaat cttccctttt ttaggtacac gggaaaagtg
    41581 agcaagcagc acagcatcta aagaaaaaga aaacactttg gaactttgga taatcttgat
    41641 atcttctgcg tataattggt cgattcgttc gtcttcgtga agcatggatt ccctccaaaa
    41701 tgaaaaaaat agtcgtctgt attatactat ttttattaaa aagaaccaag gtggcttgtc
    41761 aaacctctat tttttatgca tactatgacc ataagaaggg aaaggaagag actatgtttt
    41821 atcgatttat gcgcggggta gttcgagtac tattagcctt agttaatgga aatgcccgct
    41881 atgagaataa agaagtttta ccgcaagatg agaattacat actagttgct ccccatcgta
    41941 cttggtggga cccattatat ctggcggtag ctgcagcacc caaggaattc agctttatgg
    42001 caaaagaaga actgttcaaa aatccagttc tgcgatttat attaactcga tccaatgctt
    42061 ttccagttaa aagagaaaat ccaggaccaa gcgtcattaa aacacctgta aagattttga
    42121 aatcgacaaa tttgagtttg atcatgtttc ccagtgggac aagacatgct acagaactta
    42181 aaggcggaat ggcattgatt tccagaatgt ctaaagcgaa aatcgtgcca gctgtatatc
    42241 aaggtccgtt gacacttggg gatctgttca agcgtcagag agtgacagtg cgtttcggcg
    42301 aaccaatcga cttatcagat atcaagaaaa cggataaaga aggtatggcg gagatcgaac
    42361 gacgaacaca agcagctttt gacaaattgg acaaagaagt gaaccctgat ttcaaatatg
    42421 aagtcaaaaa aaaataaaat gataagttta tgcgtgggcg gtcttttgaa tctactgtcc
    42481 tgttcagttc agactttttc aatgaaaacg agggagagac gtcaagtctg ccctcttttt
    42541 gcgttccaat atgtaaaata gaagacaggt gtcatgtaat ggatcaaagt gttataataa
    42601 ttagaaggac attttttgag gtgattgaat gcgtttttta catacagccg attggcatat
    42661 cggaaaaaaa ctacaaggtt atgacttgat tgaagaccag cgtcatgtac tatcttctat
    42721 actagatatc gcgaaagaag aaaaagtaga ggcaatcgtg attgccggag acttatatga
    42781 taggagtgtc ccagctgttg atgccgtaga attatacaat gaattgatgg tcgattggaa
    42841 tttaaaggaa aagatcccga tttttgctat atcaggaaat cacgatagca gtacgagact
    42901 ttcagcgggt acgccatggt tcagttattc gaactatttt ttgcgaacac agctagcaga
    42961 agcatttgaa ccagtcgagt ttaaaaacgt acaatttttc tttcttcctt actttgaacc
    43021 aattgcagcg agattatatt ttgaagatga caatatccga acgatcaagc aagcggtagc
    43081 caaggtgata gataaaatgc aggagctatt tgattcagaa aaaaaacaag tattgattag
    43141 ccattttttt gtaatgggaa gtgtaaaatc tgattcggaa acgaaaatcg aagtaggcgg
    43201 gttggattcg atccctggtg aactacttac accgtttgat tatgtagcac taggacattt
    43261 gcataatcaa gctgctttga gacaggataa tgctcgctat agcggttccc cattgaaatt
    43321 ctctttatct gagatcaacc aaaaaaaagg cgtatggatc gttgacctga caacaggcct
    43381 ggagttgaat tttagggaga tcgaacccct gcatgatatt gaagaaataa cagcgagttt
    43441 caaagaattg ctggatcctg ccttttatca gtccgtcaat cgggagaatt acatctatat
    43501 ctatctcact gatagaacga tcatcccgaa tatgatgaac cagcttcggc aaatatatcc
    43561 tcgtattttg gggattgaac gggcaaacgg tcgagcagaa caaacaaagc gcaaacgaaa
    43621 agtactagaa atgaaagatc ctcaaaaatt agcagaagca ttttttagtg aaatgacgga
    43681 agagcagcta actgcaaacc aagaaaaatg gctcggagaa acgttgcaag aaatcatgga
    43741 aatggaatga tcaagaatga aaccgaaaaa attacaactt aaaaattttg gtccttttat
    43801 caatgagacc gtagattttt cacgtttgac agaagcaccg ctttttttga tcagcgggaa
    43861 gacaggagct ggcaaaacga cgatttttga tgggatcacc ttcgcacttt ttggtgaaac
    43921 ttccggaaga cttcgctctg gaaaagagat gcgttctttg tttgctacac cagacgaaga
    43981 aacaagcgtg actttttctt ttgaacatca gcagatgagc tatgaaatcg aacgaaaacc
    44041 agaacaagtt ttagcaaaac gaaaaggaaa cggaacaaaa aaacaggcag ccaaagtaac
    44101 gttgaccatt tttgatggga aaggaaaaga aataaaacaa atcacaaaac gtacagaagc
    44161 agatcagttg atcagagaac tgatgaatct cgatgcgaaa cagttttctc aaatcgtttt
    44221 actaccccaa ggagaattcc gtaatttttt ggtttcatca agcagtgata aagagactgt
    44281 tttacgtcat ttattcggta ctcaattttt ccaacagttc aatgagcgat taaaagaaaa
    44341 agcaaaacaa cagcaacaaa aattggatca tttagagcaa gagctgcatc ttctgcaaaa
    44401 aaggtttgtt ttagtcaaag aagaggaacc agcagcaggg tttgaagccg ttttagccca
    44461 atggacaaac catcaggcaa tactcgaaaa acaaatcacg gaagaaaaag aagctctaac
    44521 ggaacttcag gaaaaacaaa agcaactaga aaattcctac tatcgttttg aaagcttgaa
    44581 aaaaagttat gaagaaaaaa cgatattaat aaacaagcaa aaggaattag ctgctcaaaa
    44641 agacgaaata gaggaaaaga aacgatggat tgggtattat gaatttacgc aacgattagt
    44701 cgaaccaatc cagcgcctac aagaaataaa agttgaaaaa atagaactgt cacaaaaaag
    44761 tagtgagcaa acaaagaaac tagcgatgac tacgcagaat tatgaagagt ggcaaaaaaa
    44821 tgaagcaaac cgcgtacgcc gacaaacaaa aatcaaagaa gctgctcaaa aactgcaaaa
    44881 caaacagatg atggtgccaa ttgtcgaatc cttacgtagt aaaaaacaag acgcagaaca
    44941 attaacaatt tcattggata aagaagaatt gaatgccgag aagctgtatc aaaatctcgc
    45001 tatctatcag gaaagaactc gaattcttca agaaaattta ctggatcagg aaaaactgca
    45061 agaagatcgt cttgcttttg gacagttaaa atatctagat gaaaagcagc aagaaataga
    45121 aaaacaaaaa gcaaaagaca ctgggtcgct cgcacagata agagaagaac tcaaaaaagc
    45181 aactgaagaa ataacaaaaa atagtcagct attgaaagaa cagacagaac aagcagaaca
    45241 gctgaaaagc caatgggcaa agcagcaaat tgctcgtctt cagctttttt tgcgtccagg
    45301 ggagccatgt cttgtttgtg gatcaacgga acaccccata caacaattaa aacacgaaca
    45361 gccaaataag gaagatatct taaaagtaga acaacaactg gcagaagctg agtctgctgt
    45421 agaaagaact cagcaaatca ttgccaaaag tgaagcacat gaaaataatc ttcaacaaca
    45481 attatcagat ttagaacaaa aaatcatggc aacgaatata caattggctg aacaagaaga
    45541 acacttgcaa caggctttga aaaaggcatt tcctgagata acagaacaaa aaacaaaaac
    45601 aactcttgta gtaatcgaac aatctttaga tagacgaaaa agcaagatgg ccaatgaaag
    45661 aatagagctg gaagaaataa gacaacaagc agaaaagctc gaacaacaat tagctgtcta
    45721 caaacagcag cagacagaaa aaaataatca gctccagcaa ctgcaaggag aaatcaaaag
    45781 tttagaacaa caattgcaag atacggatag taactgttta gaagaggaga tacgacgact
    45841 tgaaaaacag ttagaagaag actcgaatca actagctgtt gagcagaaag caggagtgca
    45901 attgttgcaa gatcttcata cgcttcggac acagcttgat cttcaacatg aacaaataga
    45961 aaaaatagat gaaaaaatag aaaaggaaca agaaagaata gctaaaaagt tagaaagcca
    46021 gtcgtatttt tcaacaattg aagaaatctg tgcatttgca caacagcaag cacaatataa
    46081 agaaaaccaa cgtacagtta cttcttatca tgaagagcaa ttagtgacag ctgaccggct
    46141 gcgtcaatta gaaaatatcc agctaactga gacattccca gatacagaag agatcagcca
    46201 acagttagaa gaaataagac gtatgatcga aataaagcaa gaaaagatat acggtttaca
    46261 agaacaaaaa attagtaatc aaaaaatcta cgatgaatgt ctttcgattt atcacacaag
    46321 ccagacgaaa ttagatgagc ttacgcaatt acagcagctt tcgcaaacat tcaacggaga
    46381 aaatcctaag aagactagtt tggaacggta tgtgcttcag gtttatcttc aagaagtgtt
    46441 acaagtagca aatgatcatc tccaacgttt aacgaaaaat cgttatcaat ttgagctagc
    46501 ggataccatt ggcagttatc gagggaaaac cggattagaa atcaatatct atgatgatca
    46561 ggcaggtatg tctcgaagtg cgcataccct gtctggcggt gagagtttta ttgcagcact
    46621 ttctttagca ttagctcttg ccgatgtgat ccagacacaa gctggtggtg tagcaatcga
    46681 tgcattgttt attgatgaag gatttgggtc attagatgaa gaagctttgg aaatggcaat
    46741 ggaagcttta gaaacgatcg aaagtgaagg aagaatgatc ggcatcatta gtcatgtaag
    46801 agaattgaaa gaacgagtat tacaacaaat aagaattgat acagaaggta gcggacaaag
    46861 caaaatacgc tatcaacttg gataaaaagg gagattggta actatgaaat ggagtattcc
    46921 agaaaaagtc attgcacgtg gacgtcaata cttgcaagaa ggccgagtgt tatcggttgt
    46981 ccctgaccaa gaagaaaacg tgtggcatgc agaagtcttg ggcagtgaat tgtatatggt
    47041 tgatttggat ggtacagcaa aagagataga ttactgccaa tgtccctatt gggaggaaca
    47101 taaatattgt aagcatacag ttgctgtgga actttattta cggtcgaaaa atctatctcg
    47161 tatcatgaaa aaagaccagc caatgctaga accggtaaaa gcaacaagtg agggagaaat
    47221 cattgcaaaa ggtttttctc gtctaaaaac acaaaacaat cctcaagcgg tgcaaccttt
    47281 gattattgaa taccaagtag aaacgattga gactaatcag tatcacccag agctttctat
    47341 tttggcactt tacttgcgta tcggttcatt ggcatcaccg aaaaaaacat atatcgtaaa
    47401 aaacatctat gattttttac aagcttacac agaaaaagaa acttacactg ttaataaaca
    47461 gtaccagttt cgtctagata aacgggcgtt tcaaaaagaa gatcaaaaaa tactcacgca
    47521 tcttgcagga agtgctcaga cgcatcaatt attaggtacg aacggcttac aagtcaaagg
    47581 aaaactagat aaaagatatc tgcttctccc agtagaacaa gcccgcttcc tgttagagca
    47641 gatgaatcag actacacgtt tgcatctgca gataaatgaa caaaacgatc aaggaataca
    47701 attttctgat caaaacaaac ctttatcatt caaagtggaa aaatttggag aagattattt
    47761 gctgcaagca ttgaatgatt ttgatttttt ctttatgcat tatcagtggg gagttatcca
    47821 agggacattt tattctctga cgaaatatca acaaacggtt tatcaaactt ggaaacaatt
    47881 gattcgccga ttggagaaac cagaagtagt gttcaagaaa aaagaattac cgtctttgtt
    47941 taaagagatt atccctctgc tgtcagagat cggcgacgta gctattgccc ctgcagtaga
    48001 agaggaagta gcagatatcc ccgtcgaatt tattttcttt ttccgaaaag caaaaggaaa
    48061 aatccaagct cgagtcgatt tcgtctatgg tgaagtcatc tattcgactg acccgaagca
    48121 tgaggtctcg acagaacagg cttttcaggt gttacgtgac acagtagaag agaaacgagt
    48181 acttgatcta ttcagaatgt atcgctacca gaaaaatgaa actggttatg aacgtccatt
    48241 gccaagcggg gaagaactat atgccttttt ccgcacagaa ctatctgtct tccgtcagtt
    48301 tggtgaagtc agacttggaa gaaaattgcg agagttgtat ttagatgcgc ataaacatca
    48361 accgaaaatc gaagtaaccg agaccggttc atggctcgat attcgattcg atgtgacagg
    48421 gatagaggag caagaaatcg atcatgtcct gcaaagcttg ctgagaaatg atgcttttta
    48481 tacattagaa aatggtcagg ttctttcttt tgattcagaa gaatttcaac aaacaagcca
    48541 agtgttgcaa caattacggg aatcgatacg tacagaagaa ggaacgatcc atgtacctaa
    48601 aaatcaagga ttgatcattc aaaatcaatt ggaaaaaagc aatgctactt ttagtgaatc
    48661 gttccaaacg atggtacaag atctcattca tccagaaaga taccaggcac agcttcccaa
    48721 aggattgaat gcaacgatga gagattatca aaagcaaggt tttcgatggt tgaagatgtt
    48781 aggtcattat cagtttggcg gtattttagc cgatgaaatg ggacttggga aaacactaca
    48841 gacaattgcc tttttacttt cggaaaaaga agaacggaaa agcttctctg ctttgatcgt
    48901 agcgcctgcg agcttaattt acaactggca ggcagaggtg agaaaattcg ccccttcttt
    48961 aagtatccaa gtaatcaatg ggaataaaaa agaacgtgaa gaattactgg ctaaagacac
    49021 agatatccga gtgacttctt atgccagctt aagacaggat ctagctaatt atcagtcgca
    49081 aaaaattgat tatttgattc ttgatgaggc acaaatggtc aaaaatagca gtacgaaaac
    49141 tgcacaagca ttgagagaat tagctgtacc tcagcggttt gcactgagtg ggacaccaat
    49201 cgagaataat ttagaagaat tgtggtcatt gtttgcaacg attatgccag gatttttccc
    49261 gactaaaaca aaattcaggg aacttgccgc agaagaaatt gcgcaaatga ttcgaccgtt
    49321 tattttacgg cgagataaac aaactgtgtt gaaagacttg ccggaaaaaa cagagatgaa
    49381 tctatattct gcacttacag aagaacaaaa aactgtttat ttagcttact tgcgtcaaat
    49441 gcgtgaagaa atcacatcaa tggattctga agcattcaag aaaaaccgga tcgggatact
    49501 tgctggtctg acaagactgc gacaaatttg ctgcgatcct cgtctgttta ttgaagatta
    49561 ccaaggcggt tccggcaagc tcgaacaagt aaaagatcta ctggtagctg ccaaagagaa
    49621 caaacgaaga gttttgctgt tttctcaatt tacagggatg ctgactatct tgcaagaaga
    49681 gttagccgag ctagggatct cgacatttta tttaagaggc agtacgaaac cacaagatcg
    49741 gttaagtatg gtagacgcat ttaatgctgg cgaaaaagat gtttttctga tttccttaaa
    49801 ggctggggga actggattga atctcacggg agcagatacg gtcatcttgt atgacctttg
    49861 gtggaatcct gccattgaag aacaagctgc tggacgtgca catcggatcg gacaaaaaaa
    49921 tgtggtagaa gtgtggcgta tgatctctga aggaacgatt gaggaacgca tggattcact
    49981 ccagcaagaa aaacgcgaac tgttccaaaa agtgatccaa ggtaatgaag aacagttgac
    50041 gaaactgact gaagaagaca tccgcatgat tttaagtgta ggagaaatcg aggagtaaga
    50101 gactcgaaaa agcgttcagt aatgggtaag aaagcctttc taaagaagcg attgtttatt
    50161 tgatttaaaa aatagcaagg aggctgggat tccattttat cccagcttcc ttgctatcat
    50221 ttttttattt ctatttttaa aggttataca aagattgtct tcattgtctc taacagctga
    50281 tcaaaagaag aaatctcgat atcgcaaatt ggagtctgtt caattttgcg ttctcgatgg
    50341 ttgaaccaaa gcgcttgcca gttagcactt tttgcaccta gaacatcatt gtcgtaatta
    50401 tcgccaacgt ataaggtctc ttctggaagc atatgaaatt ctttctcagc cagctgaaaa
    50461 atctcttttt ctggtttttg gaaacctgtt gcttgagaaa tgagcatatt gcgggaagga
    50521 atccaattgt tcaattgaag ttgcttcagt ttttttgtct gatggtcagt cggcccattt
    50581 gtgataattc ctaatgggac attcttttcc ttcaagaaat cgagggtttt tttgacctcc
    50641 tcatgcaagc agatattatc aagttcttcc tcgtaaattt tttgaaataa gaggccatct
    50701 tcttccgtaa tatgagggta gtctaaatct tttagtgatt ggctgatccg atgtgctcgc
    50761 atatattcta gtgtccaatc tcctgccatt acttttggga agttttcatc actatgatgg
    50821 cggaaccgga tatatagtgg atgcatatct gcatctgaaa caagcggaac gactcttttg
    50881 accgcgtttc gaaatggctg ctgctgatca tacatcgtat catcaacatc aaacacaact
    50941 gatttaatca aaattttaca cgcctttcgg ttttcattga ttttttcaca aatccttgac
    51001 tattgtaaca gaaaaaattg tctaaaaaag ctgataaata aaggtttttc ttactatatt
    51061 tctatttcct tgacagaata aggtttttat atttcagttt ttttcactaa aaatcgtgaa
    51121 acacgtcaat aagtttgatg aacaaattaa tcattatggt agaagtcgat tgatttattt
    51181 taatgcaatc tctgtggaat tttcgaaaga tttattttt
//
LOCUS       DARBEC010000007        48683 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.007, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000007 DARBEC010000000
VERSION     DARBEC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.007"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(32..856)
                     /locus_tag="SCL80_000402"
     CDS             complement(32..856)
                     /locus_tag="SCL80_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1106294.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(829..1509)
                     /locus_tag="SCL80_000403"
     CDS             complement(829..1509)
                     /locus_tag="SCL80_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1106295.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(1532..2470)
                     /locus_tag="SCL80_000404"
     CDS             complement(1532..2470)
                     /locus_tag="SCL80_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEG1106296.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(2715..3578)
                     /locus_tag="SCL80_000405"
     CDS             complement(2715..3578)
                     /locus_tag="SCL80_000405"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEG1106297.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(3720..6293)
                     /gene="mprF"
                     /locus_tag="SCL80_000406"
     CDS             complement(3720..6293)
                     /gene="mprF"
                     /locus_tag="SCL80_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1106298.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFFEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            6434..7240
                     /gene="proC"
                     /locus_tag="SCL80_000407"
     CDS             6434..7240
                     /gene="proC"
                     /locus_tag="SCL80_000407"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEG1106299.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(7319..8875)
                     /gene="rny"
                     /locus_tag="SCL80_000408"
     CDS             complement(7319..8875)
                     /gene="rny"
                     /locus_tag="SCL80_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEG1106300.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(9286..10335)
                     /gene="recA"
                     /locus_tag="SCL80_000409"
     CDS             complement(9286..10335)
                     /gene="recA"
                     /locus_tag="SCL80_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEG1106301.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(10444..11685)
                     /locus_tag="SCL80_000410"
     CDS             complement(10444..11685)
                     /locus_tag="SCL80_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEG1106302.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(11925..12503)
                     /gene="pgsA"
                     /locus_tag="SCL80_000411"
     CDS             complement(11925..12503)
                     /gene="pgsA"
                     /locus_tag="SCL80_000411"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEG1106303.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(12712..13635)
                     /locus_tag="SCL80_000412"
     CDS             complement(12712..13635)
                     /locus_tag="SCL80_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106304.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(14374..15660)
                     /locus_tag="SCL80_000413"
     CDS             complement(14374..15660)
                     /locus_tag="SCL80_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1106305.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(15653..16921)
                     /locus_tag="SCL80_000414"
     CDS             complement(15653..16921)
                     /locus_tag="SCL80_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEG1106306.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            17173..17634
                     /gene="mscL"
                     /locus_tag="SCL80_000415"
     CDS             17173..17634
                     /gene="mscL"
                     /locus_tag="SCL80_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEG1106307.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(17694..18140)
                     /locus_tag="SCL80_000416"
     CDS             complement(17694..18140)
                     /locus_tag="SCL80_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106308.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
     gene            complement(18233..18478)
                     /locus_tag="SCL80_000417"
     CDS             complement(18233..18478)
                     /locus_tag="SCL80_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEG1106309.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(18593..19171)
                     /locus_tag="SCL80_000418"
     CDS             complement(18593..19171)
                     /locus_tag="SCL80_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1106310.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(19248..19778)
                     /locus_tag="SCL80_000419"
     CDS             complement(19248..19778)
                     /locus_tag="SCL80_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEG1106311.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(19782..20642)
                     /gene="rlmB"
                     /locus_tag="SCL80_000420"
     CDS             complement(19782..20642)
                     /gene="rlmB"
                     /locus_tag="SCL80_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEG1106312.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(20635..21030)
                     /locus_tag="SCL80_000421"
     CDS             complement(20635..21030)
                     /locus_tag="SCL80_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEG1106313.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(21027..22439)
                     /locus_tag="SCL80_000422"
     CDS             complement(21027..22439)
                     /locus_tag="SCL80_000422"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEG1106314.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(22436..22972)
                     /gene="cysE"
                     /locus_tag="SCL80_000423"
     CDS             complement(22436..22972)
                     /gene="cysE"
                     /locus_tag="SCL80_000423"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEG1106315.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(23058..23297)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(23294..24754)
                     /locus_tag="SCL80_000424"
     CDS             complement(23294..24754)
                     /locus_tag="SCL80_000424"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEG1106316.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(24924..26081)
                     /locus_tag="SCL80_000425"
     CDS             complement(24924..26081)
                     /locus_tag="SCL80_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEG1106317.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(26115..27485)
                     /gene="radA"
                     /locus_tag="SCL80_000426"
     CDS             complement(26115..27485)
                     /gene="radA"
                     /locus_tag="SCL80_000426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEG1106318.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(27548..28075)
                     /locus_tag="SCL80_000427"
     CDS             complement(27548..28075)
                     /locus_tag="SCL80_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEG1106319.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            28305..29111
                     /gene="proB"
                     /locus_tag="SCL80_000428"
     CDS             28305..29111
                     /gene="proB"
                     /locus_tag="SCL80_000428"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEG1106320.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            29108..30355
                     /locus_tag="SCL80_000429"
     CDS             29108..30355
                     /locus_tag="SCL80_000429"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEG1106321.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            30431..30775
                     /locus_tag="SCL80_000430"
     CDS             30431..30775
                     /locus_tag="SCL80_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106322.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(30892..31218)
                     /locus_tag="SCL80_000431"
     CDS             complement(30892..31218)
                     /locus_tag="SCL80_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106323.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(31833..33035)
                     /locus_tag="SCL80_000432"
     CDS             complement(31833..33035)
                     /locus_tag="SCL80_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1106324.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            33152..33667
                     /locus_tag="SCL80_000433"
     CDS             33152..33667
                     /locus_tag="SCL80_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1106325.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(33866..34312)
                     /locus_tag="SCL80_000434"
     CDS             complement(33866..34312)
                     /locus_tag="SCL80_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1106326.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(34359..35837)
                     /gene="msr(C)"
                     /locus_tag="SCL80_000435"
     CDS             complement(34359..35837)
                     /gene="msr(C)"
                     /locus_tag="SCL80_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1106327.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(36191..36820)
                     /locus_tag="SCL80_000436"
     CDS             complement(36191..36820)
                     /locus_tag="SCL80_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1106328.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(36804..37268)
                     /locus_tag="SCL80_000437"
     CDS             complement(36804..37268)
                     /locus_tag="SCL80_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1106329.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(37755..39023)
                     /locus_tag="SCL80_000438"
     CDS             complement(37755..39023)
                     /locus_tag="SCL80_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1106330.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(39254..39991)
                     /locus_tag="SCL80_000439"
     CDS             complement(39254..39991)
                     /locus_tag="SCL80_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1106331.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(40058..40627)
                     /locus_tag="SCL80_000440"
     CDS             complement(40058..40627)
                     /locus_tag="SCL80_000440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1106332.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            40755..41627
                     /locus_tag="SCL80_000441"
     CDS             40755..41627
                     /locus_tag="SCL80_000441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1106333.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            41969..42841
                     /locus_tag="SCL80_000442"
     CDS             41969..42841
                     /locus_tag="SCL80_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1106334.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            42838..44796
                     /locus_tag="SCL80_000443"
     CDS             42838..44796
                     /locus_tag="SCL80_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1106335.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(45128..45979)
                     /locus_tag="SCL80_000444"
     CDS             complement(45128..45979)
                     /locus_tag="SCL80_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1106336.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            46102..46569
                     /locus_tag="SCL80_000445"
     CDS             46102..46569
                     /locus_tag="SCL80_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEG1106337.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(46832..47314)
                     /locus_tag="SCL80_000446"
     CDS             complement(46832..47314)
                     /locus_tag="SCL80_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEG1106338.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(47414..48277)
                     /locus_tag="SCL80_000447"
     CDS             complement(47414..48277)
                     /locus_tag="SCL80_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1106339.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
BASE COUNT        13906 a        10426 c         8691 g        15660 t
ORIGIN      
        1 tcgtttttat aaggcttctt ttctttataa tttagttatt ttttcttctg aaggcaacca
       61 atattcgttt gatgatattt acaatcagga acaaagcaat aaagactaat gtgattgttg
      121 ctcctggagg tgtatctaga taaaacgagc tagttagtcc tgacagcatc cctagaaatc
      181 ctacacaaac actgatagca atcacgacat tgaagctttt gcctaatcgc ataccgattg
      241 ctgcgggaag aaccatgatt gcggatatca aaagtgcacc ggcaatcggg atcatcacag
      301 caatagccac accagtaatc acattgaaca tcatcgacat caaatgaacg ggtagcccat
      361 ccacgtgggc ggtatcttca tcaaaagtca gaacatacat cggccgtttg aataaaaaga
      421 acaatatacc gatggcaacg aataagattc ctaaaaagat gacttgctcc catgtaatgg
      481 tcacgatcga accaaacaga taggattgga tacttgttgc tgaattccct ccgctcaaat
      541 tcatcaatac taacgctagc gccaaaccac cagccattaa aatggcaatg gagatttctg
      601 aataggttcg gtagatcgtt cgcagatact ccaatatcac agctgctaat acaacgacta
      661 gcatggtcgt aagtgtagga ttaaggttca aaaagaatcc taacgctacc cctgctaaag
      721 agacatgaga aagcgtatca gccatcagcg attgtcttct gatcactaaa aacacaccta
      781 gcatcggggc gattccggca atgaagatag cggcaagaaa cgctcttctc atgaattcat
      841 atgaaagcaa cgccattgag aatcctcctt tctcaccagt cggatctgtc tgtccgcata
      901 ctgtttgata tcttcatgat catgggtgat cattagaact gatttcccat ggacatgcgc
      961 actatgtcgg agaagacgat aaaattcatt tctggattct tcatccatac ctgttgttgg
     1021 ttcatcaaga acaaataagt cagggtcagt agcaaaaata cgcgctaaac tgattcgctg
     1081 cttttgtccg ccagacaatt cacctattcg ccgatttcgc atatcccaca tccctacagc
     1141 ttctaaagca cgctgtacat gttgatgatc tttttcagag agacgcttga accagcggcc
     1201 tcttggatat cttcctgaac ggactaattc gatcaccgta ctaggaaaac ctgcattgaa
     1261 agatgcaact tgttgaggaa tataaccgat acttaatttt tttccttctt gatttttctc
     1321 agcaatcttt atcattcctt ttgaaggttt cagtaatcct aacgatgctt tcaccaaggt
     1381 tgacttggct gctccatttt ctccagttaa aatgacaaat tcacccgcat ctacatgata
     1441 agagacatct tctagtacag gttcatcatc atagtaaaag gtcaaatcag atatttcgat
     1501 ataacgcacc ttacaacctc cattctaaaa cttaattgat actttctttt aaagctttca
     1561 gatttttttc catgacagaa atataatttt cgcctgcttt gatttgttca ttagtcaagc
     1621 tttctaaagg attcaacact tctagcttca ctcctgtttc attcgacaat gtctcagcta
     1681 ccttcgaaga ggcattttct tcaaaataaa tcactttgat gttgtttttc ttcacaaatt
     1741 cttttagctc agctaagcga cttggtgtgg gttcctgatc tggtgaaacg cccgaaatcg
     1801 ctacttgttt taagtcgtat tgattcgcta aataagcaaa agcagcatgc tgagtgacaa
     1861 atgtgcggtt tgttgcatct tttagctcat cggagtattt ttgatccagt gcttcaagct
     1921 ttttgatata agcatcggca ttcttcgtaa aagtttcctt tttatctgga tatttttcgc
     1981 ctaattcttc agcaatcgtt ctgacttctt ttatagctaa taccggatcg agccaaacat
     2041 gtggatcgta ttcatgtgaa tgaccatgcc cttcttctga ttcttcatgt tcgtctgctt
     2101 cttgcgattc taaacggtcg attcctttag ctgcttcgat catcaaggtc tgtttgctat
     2161 ccactgaatc ctttaacgaa tccacaaaaa attccatgtc tgaactatta tacacgaaaa
     2221 catctgcatc ggaaatttct gctctttctt ttgcacttgg ttcgaaatca tgcggctccg
     2281 ttccagcagg gatcaaaagt ttcacgtttc cttcatcccc tacgatctct tttgtaaaat
     2341 catacatcgg ataaaaagtc gtaacgatcg tcaaatcctc ttttttgtct gcttccttgt
     2401 tcgtatttcc acatgctgca aacagtaaag cacctatcat taccgtagct gtcaaaagat
     2461 attttttcat ttatatgctc ctttcaatga acaaatcgga attttacgat ttattaacgt
     2521 gaactaatgt atcaaaataa aaaaacacag tcaatagaaa tccatcaatt tctgatttaa
     2581 gcatgtatat tcttgattaa atcgtaaaat ggatgagttg ctgctcatat ctagccattt
     2641 cactacaata aaaaactgaa gaataccatt ttaggattcc tcagtttttc atcacctatt
     2701 ttacttctta tattttattg cagggttcgt actaaataaa cttcctcaca aaaacctttt
     2761 aacccattat atactctctg cgctcttgaa taatgttgac acaaggcaaa aacggttggt
     2821 ccgcttccac tcatcaatgc agcatctgct ccgtacttga tcatacgctc tttgatttga
     2881 tggatgactg gatgccgctt tcccgttata tcttctaaac tgtttcccat ttttttgatc
     2941 atctgctgat aatcttgctc tcggatggct tcagcaagtc cgtggatgtc ttgatgttgg
     3001 atggcttgcg tatctacttc tctaaacact gtacgtgtag agacactcat tcttggtttg
     3061 accaacacaa cccagcactg tggcatagaa ggtaatactt ggatttcttc ccctttgcca
     3121 ccgacaaatg ccgtacttcc gtagacacag taaggaacat ctgtaccgac ttccaaaccg
     3181 atttctgcca gttcttctaa cgaacagccc agattccata attggttgat ccctcttaga
     3241 gcagctgcac agtctgtact tcctccgcct aatccagctg ctactggaat attcttctta
     3301 acaaagatct tgacaccggt tttgatattg tatttatttt ttagcaataa agcagcttga
     3361 taaacattgt tccgttcatc taccggtaaa aatgctttat ttgtttctac aatgattttg
     3421 tcttctgtta tttcttcaaa aaatagatga tctgccaagt ctacgctcga catcaccatt
     3481 tccaactcat gatacccatc ttgccgtcta tataatacat ccagtcctaa atttatcttg
     3541 gctggcgctt tttcaatcaa ttccatcctc tcacccattc gaaaagtcta agtcgcaagt
     3601 attctatcat agggaaaaat aaaagtatag cttatgtctg cctagtggtt gattttgtca
     3661 tcgtttcaac agctttcaca gaattttctc ttcataatcc aaacaagttc tagtgattct
     3721 tataattctg tgagcacttc ttctgccaaa ctcggaacat ttcctttttc ttttcctatc
     3781 agactgagta gctgcatggt agcaaatccg aaattacttc ctttcctata agcaaaataa
     3841 cgtccttccc aatgatcgac atattttaat ttcttttcat aagcagactg gaaggaatag
     3901 aaaaaattac cataattata tacgatatgc atgacttttt cttgaaagaa agaaaaatct
     3961 gtttctccaa cattagctaa tggagctaag cctagattgg cttccgagta gcccattttc
     4021 tcgtatacaa taaacagatt ggctaacaga tagtcagcta attcctcaga ttctttaggt
     4081 aagacccgca acaaatcata tcccgcctgc ttcttcgtca tcggctgagc ggtgatgaag
     4141 ccaacgactt tttgatcttt acggacaata ccgacaccgc tagataatac atatgattta
     4201 tcaaatcgtc ccacagagaa gtatttttct cgtgcacctt gaagccattt ttgtgaaact
     4261 tcatttacag cttgatgtaa ttcttccggc agttgatcgt accaagtaaa atggtatcct
     4321 tctttcatca aacgttgata ttcaagttgc tgactcagcc gagacaagtc ttctgttgct
     4381 agatcaacaa tccctgcttc acctactttc gtgaagtgaa aacccaattc atgaagcatc
     4441 actacgtagc tttctgatat cttataaaac gcaagctgat agcctaaaca atctgcttgt
     4501 ttcataaact gattcgttgc tgcccgcatc attttaggat tgccaactgg atctcctaaa
     4561 acaaagcatt tgtttccttt gatctgataa ccaaaaacga cttgatcttg gtcgtcttct
     4621 tggtaataaa aataactgta acctgacaag cgaagctgat ggcttgtagc tgtgcctccg
     4681 tatgtttcca ataaacggtc aaaacggtct ttttcgtact cactgccaag cttcgtagta
     4741 tcttcagcta aatattgata aagcgccact aaagctagca atgagatgcc caaaccaata
     4801 agccctgaga accacacatc atctgaagga aacaaaataa aatgatttcc tgttattcga
     4861 ccgactaaaa agcgtccgct gtgatagcct gcaattgcgt aaacgataaa caaaaagccg
     4921 aacaagctag catcaaagat tatagctccc caagaataaa cgaattttgt tcgataaaat
     4981 tcttttctgg ataaccacac agctaacaga atcaagacat ataccaatat caactgccaa
     5041 gataacgtac gtgtgattgt atttaaaata ccaaaagata gtaaagcaat cgtaggcaga
     5101 aatgcctttt tgacttttac tgaaagtgct ctagcaaggc cgagtaataa aaagccaacc
     5161 aatagattca ctgtctgatc taaaaaattg aatgagaacg gcaataatac ttgaaatagc
     5221 cgactgattg cagacagatt agtgattgtt gatagaagta ccatcatgat ccccgcaaaa
     5281 tagacagcac ttacgagcac gatatgcgca accttttgga aaaacagtcg cggaatgtta
     5341 tctaaaaatc gattgatttt gactcctgta tgtgtgatga acaatagaag tcccgtcata
     5401 aaaggtagaa cataataaaa caaccgataa tataatagcc aaactactgc tacatcttgc
     5461 cgaacagaca attgactcat tcctaagatc atcaaaacgt caaaagtccc cattccacct
     5521 gggaccatcg tcagcattcc tatcaatgta gcaatcatga acataggata gatcgaaagc
     5581 atcgaaatat ctattctcat caacttgccg atgatcaaaa ataccgcaag ggctcccgtc
     5641 cattgtccaa aggatgccag tagcagacca agcaagcctt ttggataaaa ctcgaagaac
     5701 aatgtctttc tctttagata agcaaaaaag aaaatgactg gtgctaaaaa actaccgccg
     5761 aaaagccaga tccaatagct tcgaaaaata ctttctgact gaataaaaaa gacatcaata
     5821 aatgtaagaa acgaccatac agacaaacca gtcagcataa atagtgcgac ttttgataca
     5881 gtggctaaaa ccatttttct atttgtctgc tttccataaa aactagcccg cagacttgct
     5941 cctaccacac caccaaaacc ggcgagattg ttgatcgtgt ttgttaccca cgcagcaaga
     6001 agaaactctt ttctttccat ttttggtttt ccttgttttt ctaagactct gatcgtcacc
     6061 caatcataca acaacatcgg agtgacaccc agcagtccag ccagaatcat gagcaggatc
     6121 gtcatgcggc tttgttgtcc catcctttgc caaatatctt gccaactcat gccattagca
     6181 atatttgcga cttgatttgc cacaaagact aacacggagc cgaaaaaaat cagttttaaa
     6241 atcagactat gttcctttat ccattgcagc atcaagtatc gtttattttt cacattttcc
     6301 cattcctaac ttgtatactt ttgtgtcata ccccaatgtt atcttattta ttttaatttt
     6361 tccactttag acctgttctt tgcttgcgat gatagaaaaa gaaagatatc ctaatgctaa
     6421 aggagaggat aacatgaaag taggatttat cggcacaggg aatatggcac aagctattat
     6481 tgatggttta ttaaaaaaag gaacagtctc gaaagaagag gtgttcgttt ccagtggaca
     6541 ttttgaaaat gcacaaaaat ttgctgctga aactggcgtc catgcatgca aaagtaatca
     6601 ggaagttgca tcacaatcag atatcgttct tttagccgtg aaaccaaaaa tcattgaaaa
     6661 agtattggaa gaactaagag aatatagcca gaaagtctta ttcatttcta ttgcttcagg
     6721 aaaatcatta atagatttag aaacagcgct tgaagatcct caggcctcca tcgtacgtgt
     6781 gatgccgaat gtcaatgtca gcgtaggagc aggcgtttct gcgatttgca aaaatgaagc
     6841 tgccacggag gaacagcttc agcaagctac gatgattttt gaagcaatcg gcagcgtcta
     6901 tcctttagca gaatcagatt tcagtacatt cattggcctt gctggcagct cacccgcatt
     6961 cgtttacatg ctgatcgatg cgatgggacg gactggtgta ctgaatggtt tgtccaaaca
     7021 gacagccact gagattgctg caaaagcaat tatgggttct tgtcaaaaat tcctttcaag
     7081 tgataaaaat ccatgggaac ttgtagacca agtttcttct cctggcggta caactgttgc
     7141 aggtgtagtt gccttagaag aagcaggatt catcccagca atcatcaaag gaatcaatac
     7201 gacgatcgag aaagatcttg agatgttgaa taaaaaataa tgatagcaat gttttttcgt
     7261 gccttacaaa aaaagaagag ctgtggcttt tgccgcggct cttcttttgt attttttatt
     7321 atttagcata atcagttgca cgagtttccc gaataacgat tactttgatg tgtccaggat
     7381 aatccagttc ttcttcgatt cgtttacgaa tatcacgtac taaacgaaca gcgtctaaat
     7441 ccgagatttc ttctggcttg accattaccc gaacttcgcg tccagcttga actgcgtaac
     7501 ttgattctac accggcgaag ctgttcgaaa tattttccag acttcttaaa cgacggatgt
     7561 agttttctaa tgattcactt cgcgcaccag gtcttgctgc tgagagcgca tctgctgcag
     7621 ctacaaggac agagataaca gaagtagctt ccacatctcc atgatgagaa gcgatcgcgt
     7681 tgattactac tgggttttct ttgtacttgg cagctaattc cgcaccgatc tcaacgtgag
     7741 atccttcaat ctcatggtca agtgctttcc caatatcatg gagcaacccg gcacgttttg
     7801 ctaattggat atcttcgcca agctctgctg ctaagatacc agctaatttt gcaacctcga
     7861 ccgaatgctt caagacgttt tgtccataac tagttctgaa tcgcagacgt ccgatgatct
     7921 tgattagatc tggatgaagt gtatgagcac cgacttcaaa cgctgcctct tccccgtatt
     7981 cgcgaatacg ttcatccatt tctttacgag atttctcgac catttcttcg atacgcgctg
     8041 gatggattcg tccatcttgt atcagttttt ccaaggtcat ccgtgcgatt tctcttcgga
     8101 ttggatcgaa tccggaaaga acaactgctt ccggtgtatc atcgatgatt aaatcgatac
     8161 cagtcagcgt ctcaagcgtt cgaatattac gaccttctcg tccaatgata cgacctttca
     8221 tttcatcatt cggaagggta acgactgaaa cagtcgtttc tgaaacttga tctgctgcgc
     8281 agcgttgaat cgctaatgat aataagtttt tcgctttgcg atctgcttct tctttggcac
     8341 gctgttcaga ttcttttacc attagagtta actcatgatt cagctcttct tcagtcgatt
     8401 tcatgatgac atctcgtgcg tcctcttttg ataaaccagc aattttttct agttcttttt
     8461 gttgatcttc aatcattttt tcaacgtctt tttctcgctc ttcaattaat tgctgttttg
     8521 aactaagtgc ttcttcttta ctttcaagtg agttctcgcg tttttctaac gagtcatctt
     8581 tacgattcag cgtttgttcg cgttgaatca aacgattctc ttgagatttt aattctaact
     8641 tactttctct cagctcactt tcaacttcag aacggtattt ctgattttct tccttagctt
     8701 ctaacaatgc ttcttttttt agagtttctg cttctttttt tgcactatcg atgattccag
     8761 cagctgatga ttgtgcacca gctatttctt tctcgtgacg tgactttgcg accataaacc
     8821 ctaaaccaag accgacaatt aaaccgatga tagcgaggag aatgttgaat accataaaat
     8881 ccacctccat actatctttt tcatttttat catctataca gcttctatga taaattattc
     8941 taaactcgtc aatatgcatc ttcgcatata tgtgtacaaa aaacatgcac aactttattc
     9001 taatgtttgt accccgtagt gtcaacttta aataaacaaa gaaacggctt tctacttatt
     9061 cattaaacga tggtttttaa aataaatgac actagtttca tataggtaaa cctttaaatt
     9121 gagaaacttt tctattaaat tattctttgt agatgaacag atatttttat tttatgtacc
     9181 cactcgtata attaaataac tcacgatttc tttattcaat aaaaaaacgg caggtcaagg
     9241 agagtaccta ctcttcagac cagccgtttt tgtttatcat aatttttatt cttctactaa
     9301 aggcaattct tcttgtcctt tttcatcttc tgggacagcg acttcttcac caataccata
     9361 agctgctcta actagagcag aaacttctgc catcatttca ggatgattag ccatatagat
     9421 tttagcgttt tcccgtcctt gaccaatccg atcttcttta taagaatacc aagcaccgct
     9481 tttatcaaca atgtcttttt ctactgccat gtctagaagt tctccttctt gtgagatccc
     9541 taaaccatac atgacatcta cttcagcgat tttgaaagga ggtgctactt tgttttttac
     9601 gactttgatc tttgtacggt taccaacaat atctgtacct tgtttcaatt gttctgcacg
     9661 acgtacttcc aaacggatcg ttgcgtagaa tttcaatgcg cgtcctccag gagtgatttc
     9721 tggattccca aacatcacgc ctactttttc acggatttgg ttgatgaaaa tagcgattgt
     9781 ttttgttttg ttgatcgaac cagagagttt acgcaatgct tgagacatca aacgtgcttg
     9841 taacccgaca tgtgagtcac ccatttcgcc gtcgatttct gctcgtggaa ctaaagcagc
     9901 aactgaatcg actactacga tatctacagc cccacttgat actaaagcat cagcgatctc
     9961 tagaccttgt tctcctgtgt caggctgtga aagaagtagt tcatcgatat tcacacctaa
    10021 tttttgtgca tattgcggat ctaacgcatg ctcagcatca atgaaagcgg ccgttccgcc
    10081 atttttttgt acttctgcaa tagcgtgtag tgcaactgtt gttttacctg aactttcagg
    10141 tccatatact tcgatgatac gtccacgggg gtaaccgccg acacctaaag cgacatctaa
    10201 cgcaagagag ccacttggaa ttgtagagat ttgttggtcg attttttcgc ccaatttcat
    10261 aatagagcct ttaccatagc tcttttcaat ttttttcagt gcagcatcta aggctgcttt
    10321 acgatcatct gccaatacga taatcctcct tatatttaca aatcagagca acatttcatt
    10381 atccttatca tatctttttt agtgaaaaaa agcaagaaaa aaccgaacaa atattcgcat
    10441 ttatcatttt cttaaaagct gttttctcaa aagatcaaat ccttgcataa ccgcattata
    10501 tctgatagca gcacgatctc tggcaaagtg atatttttcc gaaaccacgc cgtctttaga
    10561 tgaaatagcg atccatactg ttcctgcaga ttgaccttct aacgtgtccg gtccagccac
    10621 tccggtgaaa gctacactgt aatctgttct tgctatctca cgtgcttgaa tggccatctg
    10681 ctccacacat tctttgctga ctgttccata ttctgctaat agctccggat caatctccag
    10741 aaaaccagct tttgtttgga gcgagtaggt aacgaatccg cctggaaata cttcagatac
    10801 accagcaatg tcgcctaatg cggcttggaa ggcgccggct gtcaaacttt ccgctgctgt
    10861 tacagtctgc ttgttttctt tcagtagttc cactacgact tttgctagcg aattatcatc
    10921 gccgtatcca taaaaatatt cccctactcg ttcttggatt ttttcttcta atgctaaaag
    10981 agcttgattc cctgcctgaa catcatttgt tttcactgtc agtcgtaatg tcacttcatt
    11041 tggtttggca tatggtgcga tcgttggatt gatctgtgtc tcaatcagat cttttagctc
    11101 agtgaccagt cgcgattcac caatcccata aaagcgcaaa actttagaaa ttagtttttc
    11161 ttcagaagga aaacgttcct ctagtaaagg acgtacttgt tccacaaaca ttggtttcaa
    11221 ctcattaggc ggaccgggaa gaaggatata cgcatgtgta tctgtttgat aaaaagtccc
    11281 taaagcaagc cctgtacgat ttggtagagg aaccccgcct tcgataatct gggactgttg
    11341 cagattgttt ttggtcattt ttcgatgggt tgtttcaaaa tatgccaata attttttgta
    11401 tccttccgta ttttgtatga gagacttacc gaggtgagcc gcagttgctt cttttgtcag
    11461 gtcatcttct gtcggtccta atccgccaca taagagtatg agttcacttc ttgtttcagc
    11521 tagagcaatc aactcttcta aacgctgctg attatctcct acaactgttt gataataaac
    11581 ttcgatacct agatcagcta gctgttctga caagaaagtg gcattcgtat tcacgacttg
    11641 tcctagtaaa agttctgttc cgacagctat tatttctgct ttcattactt cccgctccct
    11701 tatatagtat acgctgtatt tttatatttt tttatttaaa aagagggcaa ggagctgtga
    11761 caaaaatctt gtcacagctc cttgttccaa ataaacagtg ttctcctgcc aaaacctcgg
    11821 tcataagtca caatatacat aaaacatgaa aagctgtttt ttgaaattat cccttgtgac
    11881 tcagagttag acggcaggct cacaacctct ccactaaacg atacttacat tgatcctttg
    11941 aacacaccgc tgtttttgat gaagtaatcc acacctgaat agatagtaaa gatcagacaa
    12001 gcataaagca tgatttgatc tactggaata ccgatcaggc tgaatgggat attattgata
    12061 aacagcaaga taatagcaac catttgtgta gccgtcttga cttttcccgg ccaagctgca
    12121 gccatgactt ctccatgttc gacaagtagt aaacgcaagc ctgtgacggc taattcacga
    12181 cagacaatga tcgctactac ccaaccaggg gcctttcctt ggtcaaccaa catgataaaa
    12241 gcagtcataa ctagcatttt atctgccaac gggtccgcaa acttgccaaa attagtgaca
    12301 aggcctcgcg cacgggcaat ttttccatct aaccagtctg tgatactagc taaagcaaaa
    12361 ataactgcac ctactagctg tgtgacagcc agtgtggtgt ctcctacgtt aaattgtccc
    12421 caatctaaag gtaaacctac aaccaaaatg aaaatcggga tcatacaaat tctaagtaca
    12481 gttaatttat ttggtaaatt cacactatcg actcctttct tccctattct acgtgactat
    12541 cagcgaagtt tccaccgata ttatttactt ttccaaatga atttattcaa aggagtattt
    12601 ttattgaaat tgatcttttt gaattaacga agaaggaccg cgacacagat ttgtcacgat
    12661 ccttcttttt tatacctaca agtaaacaga tgcgtaaact aatttatacg atcattctgc
    12721 gtattgaaga gtaaggttca cattttttgg actctgattg ttttcccctg gattaactgg
    12781 tacggattct ccattcgctt tgatcgttgc attgcttgct gcaccaatcg tgatggtcgc
    12841 atttgttgct gtttccggta acacggtact ctgtgtttca tttgctgcca gtgtcccttg
    12901 ataaacatag gcattgtcta ctagcacacc taaccataca cgattgacag cagtaaattc
    12961 aagggttaca ggcttggtag cgtctgtgat atttactgcg atggcactgc cagaatcttc
    13021 tcctgtagct attgccattt tcttttcttc acttgatttt gttgttgatt ctactgtaga
    13081 agactctttg gtctgcgtgc tttgctctga ggaagaggac tcctcgactg atccttctac
    13141 tttaagcgaa gaagctgtgt ctccgattat tggatcagct tgacgatctt gccaagtcat
    13201 atagcccacg acaacgacaa tcgccaaagc aaccaatcct aataaaatga caggaagact
    13261 tgtccaaaag cgacttggat gcgattcttc ctgatgcatt gcttttctag aagtacttac
    13321 tgtttcaggc tgcggacgtt cagggaccga tcctgtcaat ttgcttttcc catcaaaaac
    13381 atcgactaaa tggtctccat cttctcctac agcttcagcg tattgccgaa taaatgcccg
    13441 cacgtaaaat ttaccaggca attggtcaaa atcatcattg tcgattgctt ccaaataacg
    13501 tttttgaatt tttgtaatct gctgtaattc atctaaagat atttttttat tcaggcgtgc
    13561 ttgccgtaat ttttctccga tggtttcaaa agccactcgt tcatcttctc cagtctgttt
    13621 gattctccga agcatctgct gttgcaacga aagacgttat gtttgggtgc tgtgacaaaa
    13681 gtcatgtcac agctcctttt ttccgaataa acattgttct cctgccaact cctcggtcac
    13741 aagtcacaat atccaataaa tataagaagc tgttttttga acttgttcct tgtgactcgg
    13801 agttaaacgg caggctcacg acctctttct taaacggtgt tcaatcagct gttcctcggt
    13861 attagctcaa aaaccggcaa aatataagga gatatttttc cgtatgtttc tctaatactc
    13921 aggtcaaaac gctgatttca caacctcttc cttaaacggt gttcaaaagc ttgcatctgc
    13981 ggtaataagt ccaaccaagt taaaaacatt aagaagctgt ttttacttgg ctgttcttat
    14041 tacttgatgc agggcagctt tttcacaacc tcttggtaca cggtgttcca gaagtagctt
    14101 cttcggcaat aagccgaaat atcacaaaaa tttgagaagc aattttcgta aattccttct
    14161 tatttcttga agctgaccac ttctgtcaca acctctttga ttacttcgtt tgattttatt
    14221 ttaatataag ttgattaaaa gagagcatga tttgttttat tttctcacta atttttccaa
    14281 cacaactaag aataccataa aagctgattt tggaggaggt tcctactaca agtctaaaac
    14341 gaaatttaag acttttcctc ataaaatact ggtttatttt ggtctcatgt aaaactcgct
    14401 gaaggcctct tcacttataa atgcttcccc agctgccagt acgtctttca tttggatcga
    14461 atcgatgatt tcaggaagat caaataaagt tcgatcgcca aataaacttt gcgtaaattg
    14521 attggcaata tattcgatcg aatttaatga ttggaaatat tgtccaagca ttttcttctt
    14581 cagcaaatcg agatttgttt ctgacacttc cggatcatca gcgaatccaa gaatgatttc
    14641 tttgactttt tctgctgctt tctctggttc gtccgtatct ccgctgaagt ccgcaaagtg
    14701 aaattcacga tccaagctga attcaaaacc aaagctgtca tcgataattc cttgattgta
    14761 catcgctaga tagtttcgag aagtattgcc taaaatcatt tggaacaaca gattgattgc
    14821 tgttttatag cgtaacagtt ctgttccttc ttgcggcaat gtgtctaatc ctttaatacc
    14881 aagtacaaat ttatctcgtg taatggctgc ttccaatgca ctttgtttga tgatttcttt
    14941 tgtgttttca gggaaataac gtacaatttc ttgctttgga gggaaatttt ttgcttcttg
    15001 gttttcccga ataagtttca tcaatttctc aggctccatc ttaccaacta caaataaaac
    15061 catattgctt ggctgataaa acgtccgata gcacgtatac aagtcttgag ctgtgatttt
    15121 gtcgatactt tcaaccgtgc ccgcaatatc gatatgcaat gggtgtgtag gatacaagtt
    15181 atttagtatg ccaaaaaaca tccgccagtt agggtcgtct tcatacattt ggatttcctg
    15241 accgatgatt cctttttcct tgtttacagt ttcttctgtg aagtaaggtg cttgtacaaa
    15301 atcgatcaga gtcgtcagat tcttttctac ctgatccgtc gtagaaaaca gatagctggt
    15361 tttcgtaaaa cttgtgaaag cattggcgga agctccttgt ttcccaaata attgaaagac
    15421 atcaccatct tctttttcaa acaacttatg ttctaaaaaa tgggcaattc catctggaac
    15481 tttcaccttt tctttttcac catagggaat aaattcgtta tcgatcgaac cataattcgt
    15541 actgaacaaa ccatatgtct tgtgatagtc atttttaggc aacaaataca cggtcaaacc
    15601 gtttggcagt acttcatgat aaagtgtttc attgatttgc tcatattctg ttttattcat
    15661 ttgcatttcc tcctgctaaa aagaagattg cctgcaattc gatttgctca gctacttgtt
    15721 gtatctcttt gatcgttacg ctctccatac gtttcaacca ttcttcatca gataattttg
    15781 tttccggcag ccagctgtcc aaaaaagaag cttcgattgc cgcttgcgga gagtctagag
    15841 aaagcagata ttggtttctc agcattgctt ttgtctgaga cagttccaac tctgttattt
    15901 caccatttcg caaactttct aattgctcgt gaatcagatg aagtacttga tttcggttct
    15961 tttcatcgat accggtttgg acactcatga atcctcggaa cgtatccacg ctactagagg
    16021 cataataagc tagactttct ttctctctga cattcatgaa aagtttagag tgaggaaaac
    16081 cgccaaataa accgttaaag accattagcg caaaacgttc tggttcatcg taatacacat
    16141 ttgtctgata agctaggttc agctttgctt gaacgattgg ctcacgtact tgtcgttcct
    16201 tcacgatatt cacttgaggt tgggtataaa acagatctgg ataaactgtt tcccgcgact
    16261 caaatggtaa ttgcccaact gcttcagctg ctttgtccgg atcaacatcg ccgacaacaa
    16321 aaatgtcgat ttgatcattt gccatcattt cttgatagta ggacgctagc gaactagcag
    16381 tgatcttgtc caaatcttcg actacgccca accctgggat cttttgttcc gggctgtttt
    16441 caaaataaag ttcttgcaag gcataggaag caaaagtttg tttatcttcc ttcaaacttt
    16501 ccaaatatag acgcagattg ttcttttcca agtcaaacgt caactgatca aactgatgat
    16561 ttttaatatt tggataaaat aaaacctctt ttaaaaaatc taccgcttga gaaaaaaggt
    16621 ttggatcatt gacatattca ccattcacaa aactgatccc tgcgtttagc caatgtaaat
    16681 ttcctttttt tctaactgat aatccaaagc ttgcgccata aagctctgca agttgcgcac
    16741 ttaactttgt ctgatccgaa taattcaaac tatttgtttc caacatgctg ctgagaagtg
    16801 aacgtttcgt gatgacttct tgttgcagac gcgcagtaaa acggataaaa atccggatcg
    16861 ttttgtattg ttttgttggt aagacatgaa gattcacgcc ttctgccaat tgatacacca
    16921 tatttatatg ctccttttca cagttgattg cccgcttact cttttaatgg gtcaacctgc
    16981 cttttttact tattcctctg cttgaaattt ataagttcag gcaattcgcc gttttttcta
    17041 aatgatgtta cactttatag tatagcatag atgtttttta tcaaaagaaa cagacggcta
    17101 aaagcgtata ttgttttttt attttttatt tttcgatata ctaaagacaa ataaaagaag
    17161 ggagggtcat tcatgatcaa agaattcaaa gagttcatca tgcgtggaga tgtcttggat
    17221 cttgctgtcg gcgttgttat tggtagtgcg tttacaagca tcgtaaacca agtggtgcaa
    17281 ggcttgatca caccactaat aggttggttt gtcgcaatga tcacaggtac ttctgattta
    17341 gaaggagcac tttcaattct tgactggtcg ccgacaaaag gcgtcacatt tgcgtttggt
    17401 aacgttatta gtgctatcat tacatttctg atcactggtt ttgtcttatt cttagttgtc
    17461 aaagcggcaa accgtgcgaa gaatttacgc tccaaagaag agcaagtgga agtggaagaa
    17521 actcctactg cagaagatta tcttagtgac atacgcgatc ttcttgccaa acaagtggca
    17581 gaagacaata cacatccagt agagcaaaca aatctttccg atacttctaa ataataaaga
    17641 atctaaaaaa aaagaaagcc gattttcagt gtcggctttc tttttttaga ttcttaactt
    17701 gctacttgtt cctttttcat gtcgatatgt ggaatatcat cttccaaata cacatctgag
    17761 attgccttga atccaaacga ctcataaaaa gcttgtaggt attcttgtgc gccgatcacg
    17821 attggttttt ctggaaaatt tttctggatc atgttaatcg tttcttgaac aatctttctt
    17881 cctaaaccat ctttacggtt gtcctgatga acaaggacac gtccaaaatg gatactctcc
    17941 gcttcttcat atactcgcgt gtaggcaagg atcttcccat cagtatcttg aaaaaacgta
    18001 tgccaagctt ctccatcaat gtcatcaact tcttgataag gacactgttg ttccacaaca
    18061 aaaactgcga ttcgttcttt gacgatcgcg taaaactctg ctgttgacaa ttcttcaaat
    18121 ttctttacct gatattccac taagcaaacg ccccctgctt ttttattctt agcataccga
    18181 ttcgcacaca aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg
    18241 cttcagttaa aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata
    18301 ctcgttcaaa agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt
    18361 ctgttaaaac gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga
    18421 ttgggctgcc gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac
    18481 agttcacctc tactatcaac tataccacat atttttaaaa ttttcaagtt taccataaaa
    18541 aaaaaagaaa tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct
    18601 cattataaaa tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta
    18661 cttgcttgtg atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat
    18721 aataatgaaa aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt
    18781 cttttaataa aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt
    18841 ctggtccttc tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct
    18901 tcagcgcgtt ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat
    18961 aacttccaaa gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga
    19021 tccttgcttc ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca
    19081 aacgtagtaa aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat
    19141 tacgtgcagc gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg
    19201 gaggaatctt atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt
    19261 ctcattaaat cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa
    19321 ttacgtcgga aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt
    19381 ttttttatcg tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc
    19441 atccactgtt ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc
    19501 tgaccagcga ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca
    19561 tccaactggt atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca
    19621 acgatgactt ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct
    19681 cttgcatccg ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc
    19741 atgttgtagc cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg
    19801 gaaaaagcct gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc
    19861 ccggtcatcg ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct
    19921 ttgccttcat taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt
    19981 gtgccggtca tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct
    20041 tgtaccaaat tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt
    20101 gttacgatag gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca
    20161 cttgcgtctg ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa
    20221 atcaaaaaga aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat
    20281 aaatattcat aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa
    20341 gtttctaatt tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct
    20401 ttcagttctt ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc
    20461 ccttgttgta gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct
    20521 tcttcatttt gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta
    20581 ttttgttttt tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc
    20641 atttttttca ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg
    20701 ttttgtcaaa tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt
    20761 aacatcagca ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag
    20821 ttcttcttct tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc
    20881 attcgcagaa acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg
    20941 atcaatcagg tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc
    21001 taaaccgttc aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt
    21061 gtatcttcta ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca
    21121 aaatcacgat tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct
    21181 aataactctt ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct
    21241 tctccttgtg ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac
    21301 ttagcaagct catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct
    21361 tcgatgaatc gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa
    21421 ttctctgagg cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt
    21481 aaattggtgg ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt
    21541 gtggtcgcca tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca
    21601 gtgatgaaat tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag
    21661 ccattgtgca tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt
    21721 tcattttcat gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct
    21781 cctaaatgtt ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc
    21841 catggtgaat cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat
    21901 gggtcttctt tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca
    21961 tcgatggatt ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca
    22021 tcacctgaag attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata
    22081 tctggcatat gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc
    22141 gtatcttctt caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat
    22201 tcgtttgctg tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact
    22261 ttatagcctc gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca
    22321 tttccgatat ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc
    22381 ttttgtatag ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt
    22441 ctgatctcct cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg
    22501 tagcgtcgtc tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc
    22561 cgatcgttac cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag
    22621 ttgggtgtct tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa
    22681 tcacatcgtc ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga
    22741 acacgccagt tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga
    22801 attgagcatt gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac
    22861 gatgccaaaa caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg
    22921 cagccggatc gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc
    22981 actcctttcg taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt
    23041 ttaaataagt ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca
    23101 atgattatct ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa
    23161 acagtttttc ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca
    23221 ttcattgata ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt
    23281 ctgttcatcg tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa
    23341 gcaattcgat cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga
    23401 ttggcatgaa aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag
    23461 ctttgacagt tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga
    23521 gaacagtagg aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat
    23581 gttcattgaa aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt
    23641 gatacaggct aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta
    23701 cacgtccatc agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg
    23761 ctttgatgta atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt
    23821 tacttaggcg ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt
    23881 cgcccacagg tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc
    23941 ctaattcgcg atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt
    24001 tacctgtttc actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa
    24061 atgcttcata gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa
    24121 cgtgagtgat tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac
    24181 catcgcgttt ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc
    24241 ctttcaccat atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag
    24301 attctagacc ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc
    24361 ctgcgtaatg aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt
    24421 cttctgtgca atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt
    24481 gatagatttc tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag
    24541 attcatccca attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt
    24601 cgatattacg tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc
    24661 gggcaaacaa ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg
    24721 gacttggtgc atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac
    24781 tttgactaac agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc
    24841 actcagcaca aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct
    24901 taccagttgg ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt
    24961 ttattgtcga ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct
    25021 gtttgaaggg cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct
    25081 tctacgacaa ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg
    25141 gcttttacta ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt
    25201 gccaacgcat tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta
    25261 tagtcattgg ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct
    25321 acttcactga tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt
    25381 tgcaaggcat ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct
    25441 gagtccgcga tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct
    25501 aagaatccag tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag
    25561 attttatatt tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga
    25621 ctagctgttt cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg
    25681 attcgtgtcg ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc
    25741 aatacactat tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct
    25801 aaagtcaatc cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca
    25861 cttagttttt gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat
    25921 aataaaaaga aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg
    25981 tttgactgtt gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg
    26041 ccgacaatga tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga
    26101 caatcattta ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca
    26161 actatctcga ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact
    26221 tttgtgaagc cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg
    26281 atttctccag tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct
    26341 ttatagcttg aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt
    26401 accccacctg cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt
    26461 tccagcactg ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg
    26521 gcatttccga acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc
    26581 ccttccattg ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa
    26641 atctgtgaag gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc
    26701 tcattcgttg agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt
    26761 tcaccttcaa aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc
    26821 gagccttctt tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt
    26881 ttgagtagtt ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg
    26941 tctggctgcg tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg
    27001 atggcacgac tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata
    27061 cttccgaggc gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat
    27121 aagacttttc cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc
    27181 ccgatacctg gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac
    27241 acacggttca gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct
    27301 gcgatttttg ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt
    27361 gtatcttgtt cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc
    27421 aagaacttag gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt
    27481 gccattgtac cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt
    27541 taacgtttta tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt
    27601 gatctgcttt taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca
    27661 ctacatcctt gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat
    27721 tattgtaata atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca
    27781 acggattgct tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga
    27841 ttccagtagg cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca
    27901 atccatctgc gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca
    27961 cgacctcttc tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta
    28021 gattgatatt ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt
    28081 ttcctcccta aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt
    28141 acaaattctt caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa
    28201 acgactgcaa gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta
    28261 gaatagaacg aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta
    28321 aaaatgcaaa acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa
    28381 atatcaatct tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg
    28441 gaaaggaagt gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt
    28501 tgtcagaacg tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg
    28561 aactgatgaa tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc
    28621 tcgtgacaag agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg
    28681 aacagctttt gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg
    28741 atgaattaga tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa
    28801 aacttgtcgc ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata
    28861 atccgactac aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga
    28921 ttctggcaca agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat
    28981 taaaagccgc ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa
    29041 atccggcaat tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg
    29101 acttgaaatg actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact
    29161 agcacttatg cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa
    29221 aaagaatcaa caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa
    29281 caatatcagt gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat
    29341 ggctacagag atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat
    29401 gtggacgaac accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg
    29461 catcatttac gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc
    29521 agggaatgcc gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt
    29581 ccaattattg cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt
    29641 cgatgatact tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt
    29701 gctgattcct cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc
    29761 tatcattgaa acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc
    29821 tatggcagtg aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc
    29881 agaaacattg ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga
    29941 actggtagaa cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac
    30001 ggctgcttta gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt
    30061 caaagtggtc gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca
    30121 ttcagaaacg atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga
    30181 cgcggcggct gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt
    30241 tggtgcagag attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa
    30301 tgaattaact tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt
    30361 cgtgtgaaac attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa
    30421 aggtgtcaat atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat
    30481 cgctgctttt ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat
    30541 agggagtcaa cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt
    30601 tacacgcttg cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta
    30661 ttcttttttc gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca
    30721 cttatcttat aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat
    30781 atccgtacga taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg
    30841 ctgtgacaaa gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct
    30901 gcctggtaaa tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc
    30961 ataataacga agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc
    31021 tgattcagcc agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat
    31081 cgcttttcta gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata
    31141 aatcaaccga tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg
    31201 aaaatattct tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta
    31261 cctactgaaa attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg
    31321 tgtcacatcc cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc
    31381 acaatattca acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta
    31441 aacgacagtc tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc
    31501 tcaaaaaacg gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat
    31561 aaaacgctga ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag
    31621 ctcaaaaaac ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac
    31681 aaaacgctga ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat
    31741 aagcccaacc aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga
    31801 tgcaggacag ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa
    31861 ttacggtgat aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac
    31921 gcatagcttt caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca
    31981 ccgaaaaagc ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt
    32041 tccatagcaa tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca
    32101 atgctgatcg gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata
    32161 atgaataaga caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc
    32221 ggtgcaacga aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg
    32281 acttctaatt cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat
    32341 ccgaaattat ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct
    32401 ttgacacctt cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc
    32461 gctagggcta taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat
    32521 gaaccactgc ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct
    32581 gcttcataca tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct
    32641 aaagcagtag agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc
    32701 attccgatat tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct
    32761 gtcatcaatg tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc
    32821 acaccagtga ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt
    32881 aactgtgcag caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc
    32941 atacaggtag ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt
    33001 tcttgtatat ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac
    33061 atgatttatt ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa
    33121 taaagaaaat aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg
    33181 cgtgctttta tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg
    33241 ggaagcccat cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt
    33301 gaccgttttg gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg
    33361 acgtgctctt gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga
    33421 atttctagca aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct
    33481 attcactaga gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga
    33541 cctctcgcta gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat
    33601 gctttgtcag caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt
    33661 tgagtaaggg ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa
    33721 taaaagagca atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg
    33781 ttcggatcac tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg
    33841 ccttttttca aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc
    33901 ttgaaagcgg gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc
    33961 aagcattcat ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga
    34021 ataaagacat acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt
    34081 ggaccttgct ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga
    34141 tgatgaagac atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac
    34201 ttgataaatg aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca
    34261 aaaaacacat cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc
    34321 actcctttaa atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact
    34381 ttttttattt tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa
    34441 gaatcatgag aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct
    34501 tcgatcgttg ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc
    34561 ttagtaaaaa gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac
    34621 ggtttcgtca aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat
    34681 tctgaaaact ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga
    34741 taaccctttt gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct
    34801 tgtttgcgga tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg
    34861 atttttttac ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt
    34921 tccaattgga cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt
    34981 actggaaaac ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt
    35041 cttctctcta atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta
    35101 gaggaagaca accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc
    35161 aacttttccg cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc
    35221 atgatcgact ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag
    35281 gcactataat tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat
    35341 gctagctcat ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa
    35401 cgaagctctt caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct
    35461 agaagcatcc cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc
    35521 cctccgctcc atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat
    35581 tccaaattta ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg
    35641 atctctgttt ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa
    35701 gtcgatttac cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga
    35761 agagaatcaa tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt
    35821 actgctaaat tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata
    35881 ctaaaaaaga gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg
    35941 aacgaaataa ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg
    36001 ttcgaaaaaa tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt
    36061 atttagttgt tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc
    36121 tcctttttta ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac
    36181 tgaaaacctt ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac
    36241 cttcttctac cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca
    36301 aaccaaatgc ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc
    36361 catgatcacg cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta
    36421 catcgttcat ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt
    36481 gatctctgac ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc
    36541 catattcgta acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt
    36601 gtctcgtatt gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga
    36661 ccacaaagcg ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca
    36721 tttccagcat ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc
    36781 taccagtcat aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt
    36841 tctgttgtta tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc
    36901 atttctacat cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca
    36961 caatgcttga ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc
    37021 tgaggatttc tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta
    37081 actgctcctt gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct
    37141 tctttcagtc tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag
    37201 atctggacga ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct
    37261 aaagacataa tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata
    37321 gaataaaaaa agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag
    37381 aaaagtatag agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac
    37441 aacgctaaaa ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta
    37501 gataatttta caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat
    37561 tagaaattgg atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca
    37621 accttttttg agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac
    37681 aaaaaacatg aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt
    37741 ttcactacct ctttttaata accataaaat cctattcctt catatctcca gcccatcggt
    37801 gttagccaat ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt
    37861 ggattaaata gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa
    37921 cgccaaccga tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct
    37981 ccactgtaag gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca
    38041 atcccttcat attcccagcc tacagttggc agataatttt tttctccggc attcaatgta
    38101 tataaatgtt ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt
    38161 gctgtatcat actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa
    38221 ttaggcgcag tggcataacg acctgttagc caagccgtag catctttgta agagttcgta
    38281 ttgcttttcc atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat
    38341 accgttacgt tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc
    38401 accgtaatcc attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta
    38461 tagcttcctt taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc
    38521 cagccacttt caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt
    38581 gccaaacttt gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat
    38641 gagcgagcat aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg
    38701 gtatgtgtca atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga
    38761 ttgttctgag cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt
    38821 gttggttctt cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct
    38881 gtatcgtttt caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc
    38941 gattcttcag ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta
    39001 aataataaat tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat
    39061 aacagtgtaa ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc
    39121 tttttaataa atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat
    39181 tcacgcctta aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat
    39241 tatccgttat ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca
    39301 tccggtctgg cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc
    39361 cccattcttt ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat
    39421 ataattcagt ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt
    39481 gctcttgacg taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg
    39541 cggaatcagg ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt
    39601 tttgtttttc catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga
    39661 tatcaagcat attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga
    39721 ttcctgcatt attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa
    39781 ctgcttggac ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg
    39841 gtaaagattc ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag
    39901 cccctttttc tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga
    39961 tgacaatcac tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc
    40021 ttgcttatac tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt
    40081 tgacgatttt ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag
    40141 ttgcattcgc tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc
    40201 ctgctccgat ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt
    40261 cttttggatc ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg
    40321 aatcttctaa acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat
    40381 ttttccctat actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg
    40441 tatcatctac tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat
    40501 ggtacccagt attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca
    40561 cttcaaagag cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc
    40621 ttgtcatttt gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat
    40681 acttataaaa catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa
    40741 acaggaggga aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa
    40801 aaaatatcag ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac
    40861 tgaaagattt agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac
    40921 agttgacgga ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag
    40981 aaaaaacggc gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg
    41041 gaggcgaaac taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc
    41101 atcctgatat ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg
    41161 atagcggatt acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt
    41221 catttcgact gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag
    41281 caaagcaaac agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc
    41341 aaacgtcagt ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga
    41401 tcgtcggaac atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg
    41461 gtgcattagc tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac
    41521 tcgatccgcc attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc
    41581 ctgctgctcg taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt
    41641 ccttcgcttt cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa
    41701 cataagtttc ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa
    41761 ttataaaaaa agaattgaca aaacataact gtctatataa aattgattac gttaacaata
    41821 attcgtttca tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc
    41881 ctaggaggtt tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga
    41941 aaaccaaatc ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc
    42001 agtattggta gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga
    42061 cagaaaaaga aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc
    42121 agaaggtcaa atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc
    42181 agcagaaacg atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt
    42241 attgatcgct ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt
    42301 catccgcaac aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc
    42361 tcaatgggca gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc
    42421 aggatatatc gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag
    42481 tatccgtgaa gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac
    42541 agatatccat tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact
    42601 gcttctacaa cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat
    42661 ggtcaaacga aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac
    42721 caaagaatac cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga
    42781 gcgtttaaga agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg
    42841 aaagcatatc tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct
    42901 gctgctttac tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct
    42961 cattttttac aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt
    43021 gggttagcac tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc
    43081 gcatttgaag ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc
    43141 aaaccagaat cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt
    43201 gccggattga ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct
    43261 ttatctttct ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg
    43321 atcagtgcag tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct
    43381 aaattcattt tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta
    43441 ctgatcccta caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga
    43501 atcaatgata ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat
    43561 caagctggat ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac
    43621 agaaatgcac gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt
    43681 gcttcattct ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg
    43741 ccattatatg tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc
    43801 cattggacag caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa
    43861 aatccgattg cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt
    43921 tactactttg gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag
    43981 gaagcagatc tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca
    44041 caagcaagca aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac
    44101 tgtactacac gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc
    44161 aaagccactg gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta
    44221 ggtacagaag tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca
    44281 cgacctgcac aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa
    44341 caagatattt acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat
    44401 gtattcgcgg aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc
    44461 ttctctccaa tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc
    44521 caaacagatg ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa
    44581 ggcgaaccat tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt
    44641 gctttagttg atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta
    44701 ttcacaaatg gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca
    44761 aatgataaaa tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt
    44821 tatcgataaa caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa
    44881 aaacggcgtt ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga
    44941 gctatttttt atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg
    45001 ataaacggtg ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga
    45061 aagccatttt cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct
    45121 ttatacatta ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt
    45181 ttcccaatac tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat
    45241 gttggatcgc taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt
    45301 cgacaaacgc tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta
    45361 atgccttggc taattcttca tagtcagtta gattgccgga taattctaag atttctggag
    45421 agtcactttc tactagtact tttgctgcag cttctgcata ttcacgtttc aatgcccaac
    45481 ctgtttttcc atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga
    45541 taggtaattc attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat
    45601 ccttaatcat tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg
    45661 catctgcaaa acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta
    45721 cattcttatg ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta
    45781 ttcctttcaa cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa
    45841 atccagcttc tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat
    45901 tttgtggaac catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc
    45961 ctgttactaa ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag
    46021 ctgtacattt ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa
    46081 aaaatgaaag gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta
    46141 cttgcctata ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt
    46201 agtatcgaaa caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt
    46261 ttgatcttta caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt
    46321 tctttactag atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca
    46381 aaaacaaatc caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac
    46441 gctcggctgc agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata
    46501 cataaaatag ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga
    46561 tttttataat tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc
    46621 ttttattctc cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt
    46681 caaagcgctg aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt
    46741 agttctgaaa aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg
    46801 tcaaagcgct actttcacga cctctatatt atcatggttt attgactacc ggtatccaga
    46861 tttctcccat atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa
    46921 cataggcgta atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat
    46981 ttcttaattc ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga
    47041 atgggatgat tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca
    47101 tcattttccc gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt
    47161 tcaatgcatc tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag
    47221 ccaaaccgac aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa
    47281 aatctggttt ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa
    47341 gttcatctta cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat
    47401 ttttattttt taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac
    47461 tgaaatcctc tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg
    47521 aagaaggaag ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg
    47581 gccctttatt aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag
    47641 cgtacggatt ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt
    47701 ccaagcgggc tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc
    47761 ctaacttatg catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt
    47821 cttgattgac tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact
    47881 gttctttctt ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg
    47941 tcaagcgtgg aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg
    48001 accaatcacg aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg
    48061 tttctgtcac tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt
    48121 tgatatagtc cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt
    48181 cagagattcc tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt
    48241 cgatgtaatc gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc
    48301 tttttatcat attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag
    48361 atgtagaaaa tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa
    48421 ttggtattat ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca
    48481 ttctaagtaa acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc
    48541 tactgtacaa agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt
    48601 tgcgatacat catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact
    48661 tcatttttgt cagataaata aaa
//
LOCUS       DARBEC010000008        48000 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.008, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000008 DARBEC010000000
VERSION     DARBEC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.008"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            248..1021
                     /locus_tag="SCL80_000448"
     CDS             248..1021
                     /locus_tag="SCL80_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1106340.1"
                     /translation="MHGDEWQLVAKKNDGWLFFFTSIFIGAMLLIGGKQGLFALGSLI
                     INIVLLLLLLLIFTKTEGISLLNISILFVVIGMIISILALDGWNRSSIIKISAAVTSV
                     FLAFFICLLTMNHWKDQGLRYEELNYLTRPYRSVFLSSVLIGTAGGTLDTVITVIATL
                     EELTKRQPEITAKQLWLSGRQVGRDVIGSMTNILLFVYISGSVPSLLLYLGNQWSFKE
                     TIEQHLSLEFLRVIAGSLGIVLSIPISVLFFLTVRRLKR"
     gene            1018..1785
                     /locus_tag="SCL80_000449"
     CDS             1018..1785
                     /locus_tag="SCL80_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEG1106341.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            1885..3453
                     /gene="liaX"
                     /locus_tag="SCL80_000450"
     CDS             1885..3453
                     /gene="liaX"
                     /locus_tag="SCL80_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEG1106342.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEIAQVQL
                     STTTGSIYLKDTDK"
     gene            3750..4070
                     /locus_tag="SCL80_000451"
     CDS             3750..4070
                     /locus_tag="SCL80_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEG1106343.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            4070..4420
                     /locus_tag="SCL80_000452"
     CDS             4070..4420
                     /locus_tag="SCL80_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEG1106344.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            4587..5525
                     /locus_tag="SCL80_000453"
     CDS             4587..5525
                     /locus_tag="SCL80_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEG1106345.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            5540..6370
                     /locus_tag="SCL80_000454"
     CDS             5540..6370
                     /locus_tag="SCL80_000454"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEG1106346.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            6414..7439
                     /locus_tag="SCL80_000455"
     CDS             6414..7439
                     /locus_tag="SCL80_000455"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1106347.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGIGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQMMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            7454..8392
                     /gene="galU"
                     /locus_tag="SCL80_000456"
     CDS             7454..8392
                     /gene="galU"
                     /locus_tag="SCL80_000456"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1106348.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKTLEAEGSKIDHNPKGITEIKEEKKK"
     gene            8517..8957
                     /locus_tag="SCL80_000457"
     CDS             8517..8957
                     /locus_tag="SCL80_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEG1106349.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            8960..9562
                     /locus_tag="SCL80_000458"
     CDS             8960..9562
                     /locus_tag="SCL80_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEG1106350.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDSKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(9621..10727)
                     /locus_tag="SCL80_000459"
     CDS             complement(9621..10727)
                     /locus_tag="SCL80_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEG1106351.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILTGTDSYTDLTSIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            10937..11938
                     /gene="ccpA"
                     /locus_tag="SCL80_000460"
     CDS             10937..11938
                     /gene="ccpA"
                     /locus_tag="SCL80_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEG1106352.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(12275..14620)
                     /locus_tag="SCL80_000461"
     CDS             complement(12275..14620)
                     /locus_tag="SCL80_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEG1106353.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAK
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            14899..15801
                     /locus_tag="SCL80_000462"
     CDS             14899..15801
                     /locus_tag="SCL80_000462"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEG1106354.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAEKGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQTFDPEVFPELLAEEVIL"
     gene            16014..17789
                     /locus_tag="SCL80_000463"
     CDS             16014..17789
                     /locus_tag="SCL80_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEG1106355.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSIYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYGSSRKLLPWFLGLSVAFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEVKKLKKRLFLLSKREWLKLSLFLLPIVGFSIVYQWIEYKNETTYGIATIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALAASPTFHTVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYHKGT
                     IKKKKEFYLMGSGDGKTMSDLPIVFDFLQSGYAYAWRYKDFHQGGNYSFGTQEQVKQA
                     QELLNLPLMENWIDSNEPRTPNFQLTKSAKVANVIIKIYQVLAIPVLIASIAGILLMI
                     LGTVFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            complement(17855..19441)
                     /locus_tag="SCL80_000464"
     CDS             complement(17855..19441)
                     /locus_tag="SCL80_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEG1106356.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVSQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQNEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHAALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFIVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADHIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQLLQETVEEIE"
     gene            19692..19916
                     /locus_tag="SCL80_000465"
     CDS             19692..19916
                     /locus_tag="SCL80_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEG1106357.1"
                     /translation="MLSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            20058..21809
                     /locus_tag="SCL80_000466"
     CDS             20058..21809
                     /locus_tag="SCL80_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1106358.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGVFSLFAVALVQLVPPKVIGIIID
                     EIADKNLSIKIIFGWIFLLIIAAFLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGRSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     QMWEKQQLEAKIEGGEA"
     gene            21809..23593
                     /locus_tag="SCL80_000467"
     CDS             21809..23593
                     /locus_tag="SCL80_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106359.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYIRVKLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFLAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     VLTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSIFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQMITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPAITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLAKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGI"
     gene            23919..24770
                     /locus_tag="SCL80_000468"
     CDS             23919..24770
                     /locus_tag="SCL80_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1106360.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTITIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELFQVIQKKYKDTFGCVEKIADFLAETFNYQMSTDEKMYLTIHTARL
                     VHENRCK"
     gene            24824..26575
                     /locus_tag="SCL80_000469"
     CDS             24824..26575
                     /locus_tag="SCL80_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1106361.1"
                     /translation="MAKPSTNKLEGIEMGKYQELARKIVENVGGKENINGLTHCITRL
                     RFKLKNEEKANDEILKNMDGIVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAV
                     STEKMRPFDMLIDGISGCFQPFLAILAAGGMIRGLTAFLVFLGAFDRGSGTFVMFDNI
                     GDSVFQFMPVIIGLTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSG
                     TIFEAPIYQTVFGIPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFF
                     TVLITMPLGFLLVGPVFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWA
                     IVPMGLMQFSVNGWQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVG
                     ITEPAIYGFTLPRKKIFIFTCVGGALGGAYSGLMNLTSWNQGGLGIFTIPNYIRPDGD
                     LTDVINVLIGIAIAMVVSFTLTFFFWKDEAGETDDIQKKSGKEIVKTPIQGQIAPLNA
                     AKDAAFAQGTLGRGILIYPEKGEVRAPFDGTVMTLFPTKQAIGMVSETGLELLIHVGL
                     DTVQLEGKYFESLVQQG"
     gene            26579..26749
                     /locus_tag="SCL80_000470"
     CDS             26579..26749
                     /locus_tag="SCL80_000470"
                     /inference="COORDINATES: protein motif:HMM:NF012578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1106362.1"
                     /translation="MKQGDLLLRFDKKKIEKEGYSLETPVIVTNYTDYLDILENQGET
                     VGPDDALITALT"
     gene            complement(26789..27691)
                     /locus_tag="SCL80_000471"
     CDS             complement(26789..27691)
                     /locus_tag="SCL80_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1106363.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQAIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIHDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSAMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVE"
     gene            27922..29325
                     /locus_tag="SCL80_000472"
     CDS             27922..29325
                     /locus_tag="SCL80_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1106364.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPAMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEENGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMDELFIAHQQYLPQFKETISRLKEEGIEVQ
                     DELARELSGNTHIMEEV"
     gene            29325..30758
                     /locus_tag="SCL80_000473"
     CDS             29325..30758
                     /locus_tag="SCL80_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEG1106365.1"
                     /translation="MGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGKDRFPV
                     ITGKKKMFEFDEDHFYPAKIAIDMYHRYKEDIALFAEMGFKTYRMSIAWSRIFPMGDE
                     KEPNEAGLKFYEDAFKECQKHGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENLC
                     RVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEKGENEEQIKYQAAHHELVASAI
                     ATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPAY
                     ALKEMERNGIQIEMEPGDAALLKEHTVDFISFSYYASRVSTTDNELLEQTAGNIFASV
                     KNPYLQASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDYR
                     IDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNQGNG
                     TLARSKKKSFNWYKKVIDSNGENLSND"
     gene            30903..31829
                     /gene="trxB"
                     /locus_tag="SCL80_000474"
     CDS             30903..31829
                     /gene="trxB"
                     /locus_tag="SCL80_000474"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEG1106366.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYESVSRFGTENAYGIVQDIKDHGSHKEVICEDKSYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNMPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            32043..33770
                     /locus_tag="SCL80_000475"
     CDS             32043..33770
                     /locus_tag="SCL80_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEG1106367.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAKHSGLITIIGEEVDAAYLEEIKAV
                     TIDRELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPDGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYFAEKTISVTMSGQEGSAKISALMEKFRKEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGSSTEDAAEKVASYE
                     AIIKKMTEE"
     gene            33785..34135
                     /locus_tag="SCL80_000476"
     CDS             33785..34135
                     /locus_tag="SCL80_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEG1106368.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKSKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAITQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(34227..35087)
                     /locus_tag="SCL80_000477"
     CDS             complement(34227..35087)
                     /locus_tag="SCL80_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEG1106369.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPTVEFNLFRRT
                     FVTDEKTLRQALQDALEEKALVLHTLINQELIEITRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEKSD"
     gene            35179..35607
                     /locus_tag="SCL80_000478"
     CDS             35179..35607
                     /locus_tag="SCL80_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1106370.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            35769..35945
                     /locus_tag="SCL80_000479"
     CDS             35769..35945
                     /locus_tag="SCL80_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEG1106371.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            35972..36418
                     /locus_tag="SCL80_000480"
     CDS             35972..36418
                     /locus_tag="SCL80_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEG1106372.1"
                     /translation="MSLLSRLNDDMKAAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(36572..37951)
                     /gene="brnQ"
                     /locus_tag="SCL80_000481"
     CDS             complement(36572..37951)
                     /gene="brnQ"
                     /locus_tag="SCL80_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1106373.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGSNWI
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYDSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFQPMGNLNQSAADSYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMPLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKSPFVYQVTMFFSAVPAVLDM
                     LANSPALVSQQTVVASLLGFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            38657..39628
                     /locus_tag="SCL80_000482"
     CDS             38657..39628
                     /locus_tag="SCL80_000482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEG1106374.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            39652..41844
                     /locus_tag="SCL80_000483"
     CDS             39652..41844
                     /locus_tag="SCL80_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEG1106375.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIICGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQKRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDAINKSYDEKIDKAKEDENIPKPTTEERIAALKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEEQIQYLNVTPAQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            41861..42337
                     /gene="ybeY"
                     /locus_tag="SCL80_000484"
     CDS             41861..42337
                     /gene="ybeY"
                     /locus_tag="SCL80_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEG1106376.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            42321..42716
                     /locus_tag="SCL80_000485"
     CDS             42321..42716
                     /locus_tag="SCL80_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEG1106377.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            42731..43630
                     /gene="era"
                     /locus_tag="SCL80_000486"
     CDS             42731..43630
                     /gene="era"
                     /locus_tag="SCL80_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEG1106378.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            43773..44585
                     /gene="recO"
                     /locus_tag="SCL80_000487"
     CDS             43773..44585
                     /gene="recO"
                     /locus_tag="SCL80_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEG1106379.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            44969..45886
                     /gene="glyQ"
                     /locus_tag="SCL80_000488"
     CDS             44969..45886
                     /gene="glyQ"
                     /locus_tag="SCL80_000488"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEG1106380.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            45888..47963
                     /locus_tag="SCL80_000489"
     CDS             45888..47963
                     /locus_tag="SCL80_000489"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEG1106381.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
BASE COUNT        15651 a         8510 c        10168 g        13671 t
ORIGIN      
        1 aattagcatc atcactatat tttttatttg tgtgcttact tcatgggtta ttcagcatca
       61 agccgcttta tatgaagtac ctgtcatcga gatagaaaaa gtacgaataa aagaggatca
      121 tcaattgatt acaggaaaac tgatgaacac ggatcaaaaa ggagaatcga tttatttatc
      181 ggttccttat caaaaaaatc aaatagaaac agcaaatata aaagcgagac aaatgtatct
      241 agtccaaatg catggagatg aatggcagct agtagcaaaa aaaaatgatg gctggctttt
      301 cttcttcaca agtatcttca ttggcgccat gttgcttatt ggtggaaaac agggtctatt
      361 tgctttagga agtctcatca tcaatatcgt tttactttta cttttgttgc tcatctttac
      421 taaaacagaa gggatttctc tattaaatat atctatcctg ttcgtcgtta tagggatgat
      481 tattagcata ttggctttag atggatggaa tagaagcagt ataatcaaaa tttcagctgc
      541 agttacgagt gtttttttag cattttttat ttgtcttttg acaatgaatc attggaaaga
      601 ccaaggactg cgctatgagg aactgaatta tctgacacga ccgtatcgtt ctgtttttct
      661 ttctagcgta ctgattggaa cagctggagg aacactcgat accgttatca cagtcattgc
      721 gacattagaa gaattgacta agcgacaacc agaaataaca gcgaaacagt tatggctttc
      781 aggaaggcaa gtgggtcgag atgtgattgg ctcgatgacc aatatccttt tgttcgtcta
      841 tatcagtggg tcagttccat cgcttctttt atatttagga aatcaatggt cgttcaaaga
      901 aacgatagaa cagcatttat cattggaatt tttacgcgtc attgctggca gcttaggaat
      961 agtattatcg atccctatct cggtcctctt ttttctgact gtaaggaggc tgaaacgatg
     1021 aatgtactta ttatacttgt tgggatttta gcaatcagcg tgctacttat tggcggtacg
     1081 caaggtatgt atattctttt aggcttattc atcaatttag ggatcttttt cctgttgtta
     1141 ttcggttatc atcagaagtg gccgatactg gcattgtcgg tcataggatt tttattgatt
     1201 gctgtggtta tcttattttt tatcaacggt tataatctaa aaatgcgtgc agcttttgtg
     1261 agtatactga tctttttatt ttgtttccta ctattgcttc ctatcactga ttttttagca
     1321 atccaaggtt ttacatcggt agaactagaa gaattatctg gcttagataa gactttagcg
     1381 attgatttta gattacttac gagatcgttg cttctgatta gtcttagtgg tgctgtttta
     1441 gatgcgagtg tagcaatttc tagtgggaca ttcgaagtat atcaggcaaa tccgcaatta
     1501 tctttttacc aattgcgcca ttctagtttc gctattgcta aaaaaatatt ggcgtcaaca
     1561 gtaatgacac ttctttttgc attcatggga agttctttag cgttgatcct ctggtttatt
     1621 gatttagaca ttccttttca gcaagtgatc aatgaaaaat catttgttct agaatatacg
     1681 atggctattc ttaccacatt gtccgcacta cttgttcttc ctttaacctg cgtgactaca
     1741 gcatatctct ttacaaaaaa gaaagaacaa ctcaagatgg agtaaaagtt acaacttaag
     1801 tcctatgaca tttttactga atcagtgcat aatatggatg taaggaaaag cttgcaaaca
     1861 attttgctta aggaggcaat ttccatgaaa gaaagagaac gtattttaga tttagtgaaa
     1921 aaaggaatcc tttcgactga agaaggctta gatttattag aaagcatggc aactgaaaaa
     1981 gacgaaaagc aaatcaaaaa agaagcagac aaggtaactg ctagtcataa agaaaaagat
     2041 caggcaagtc aattgatcga taaacttgaa aacggcgaag aagaaatttc tgagccagtt
     2101 gatccaaagg aaagagaaag acaagatcaa gaaaacttag aaaagatctt agatgaacta
     2161 gcaactgaag cgaataaaac ttcggcgcgt ctagatgaag taaatgctca attcgctgac
     2221 aataaggcag cacgcaatga gaaacaggaa gcattgatgc agctgaatac aaaagaagaa
     2281 ttaggcgagt tgacagaaga agaattagca cagcgtcaga cgttagaagc agaaatcaaa
     2341 gaattagaag ctagtggcga tacactgtta gaagaacaat tgaaattaga agcagaatta
     2401 aaagatatcc gtaagaatca atggtctgag aaaaaagaaa cgtttactga taagtttgaa
     2461 ttgccagatg attggaaaga tcaagcaaca gatacactga atcaagtagg cgaaaaaatg
     2521 tcagaagctg gatcacagtt aggcaaattc ttgaagaaaa cattccaaac tgtttcagaa
     2581 acagtgaatg acaacatgga atggaaagat gtcagcttac gtgtccctgg gattgcgaca
     2641 acgaaatttg agcatgaatt ttactatgaa gctcctgcag caagtatcct tgatatcaag
     2701 gcagcaaacg gaaacgtgac gttaaaaacg tgggacagca acgatgtgaa agttgaagct
     2761 aagatcaaac tttacggaaa aatgggtgct gaaccatttg aagcattctc tgaacggagc
     2821 cagattgagg ttaatgaaga tcatatctcc ttccaaattc caaataaacg tgtgcgtgca
     2881 gaccttgttt tctacttacc taaacgagta tacgaccacg cagctattaa acttctaaat
     2941 ggcaacatca tgattgaaac attggaagct aaagatattt atacaaaatc tacgaatggt
     3001 aacatcatcg tggaccaatt aactgcaacg atgctggaag tagaaggtgt aaacggcaat
     3061 atcgatattc gaaacgggaa catcttagat tctattatcg aaacagtaaa tggtacagtg
     3121 acatttggtg cgacagcaga aaacctttct gtgtctttgg taaatggtga tgtacgtctt
     3181 acgattaaag aagataattt gaaaaaagta gaagcaagtt cagtgaacgg aaatgtcaaa
     3241 gtcgctttac cagatggcat cggtttggaa ggacatgcta aaacaagtct tggaagtatc
     3301 aacagccgtc tgtccaatta cgaagtagtt cgtgagaaaa aagaacggac aaaccaaatg
     3361 ttgcaattcc gtcgtgtttc agatggtgaa atcgcgcaag tgcaactttc aacgacaaca
     3421 ggaagtatct atttaaaaga tacagataaa taaagaggtt gtgaaagaga tgttcagcct
     3481 tgagcaataa gaagcgattt tgtaaaatag cttcccctat tttacaaaat tgtgccttat
     3541 tgccgatagt ctatctcttg aacaccgttt accaagagat tatgaacctg ccaattatcg
     3601 ccgagtcaca aggaataatt tcagaaaaag ctcgtcacgt tttatgaata tggtaatttg
     3661 tgaccgagaa ttttgcagaa agacaccgat taagaagaag gttgtcacaa cgattttctt
     3721 catttaaaat agtagaacag aggtgagaca tgatgaaaaa aaagttgaca aaatctaata
     3781 aaaatgttgt tttgacaggg actttagcag gaataggaga atacatcggg attgatccga
     3841 ctgttatccg tgtgctttat gtattcctga gcttagttgc ttttggaagt ccaattattt
     3901 tatacatttt attagctttg attatcccat ctgcgcgttc tgcacgaaca aactatggac
     3961 ataacaatgc atattatgaa aaaaacaatt atcgtgaaca aactcgtgaa caaacaaaaa
     4021 aacgcaaaga agcggaaaaa atcgatgatg acgaatggag tgacttttaa tgtcgtactt
     4081 tcaacgtatt ttagttaata cgttaacatt catatcattg gctgttcttt ttcctgcgat
     4141 ggtccatgtg agcagtattt ggacggccgt tcttgccagc ttcgtattgt ctgtgttgaa
     4201 tatgtttgtc aaaccgattt tgacgatact gtccctgccc tttacattag tcacattggg
     4261 attgtttacg tttgttatca atgcagcgat tcttaaaatg acctcctttt ttgtgggagc
     4321 agaacatttt agtttttctt cattcggtgc agcgttgtta gtagctatct tgatgtcgct
     4381 tgtcaatgta attgtcagcg aacataacag tagaaaataa aagaaagctg tgccgaacta
     4441 tcggcgcagt ttttttattt agaatcagga atgcgttttc tattacattg gtaattaaat
     4501 aaaaacgttt tttttaattg gtagtacttt tggctagaca atgataaaat gatagagaac
     4561 actctaatag aaatgaggta gcatgaatgt cagaagttgt aaaagttagc caattagtga
     4621 aaaaattatc tttggaaatc gtgacaggcg acgagaaaag cttagaccgt gtcatcgtaa
     4681 ctggggacat ctcacgccca ggactagagt tgactggtta ttttaattat tattcccata
     4741 atcgtttaca gctatttggc agtaaagaaa tcagttttgc ggaacggatg atcccagaag
     4801 agcgtttgat catcatgaga agattatgta gtgaagatat gcccgcattt attatttcaa
     4861 gaggattacc agctccagaa gaaatgatcc aggcagcaaa tgaaaatggg atagctgtac
     4921 tgcgttctcc catctcaact tctcgtttat taggagaatt atccagttat ttagatggtc
     4981 gccttgcccc tcgaacaagt gtccacggtg ttttagtgga tgtttacgga ttaggtgtac
     5041 tgattcaagg tgacagtggg ataggaaaaa gtgaaacagc gttggagttg atcaaacgcg
     5101 gccatcgatt gatcgcagac gatcgagtag atgtttatca gcaagatgaa ttaacggtga
     5161 tcggagaacc gcctaaaatc ctggaacatc tgattgaaat ccgaggaatc gggatcattg
     5221 atgtaatgaa tctttttggt gcgagtgcgg tacgtggctc tatgcaagtc cagttggctg
     5281 tttatctgga agcatgggca aaagataaga aatacgaccg tctgggctct gaagacacga
     5341 ccgttgaaat cgcggaagtg ggcattccac aaataaaaat ccctgtcaaa acaggacgaa
     5401 acgttgcaat catcattgaa gtagccgcga tgaatttccg cgcacgtaca atgggctttg
     5461 acgcaacgaa aacatttgaa gaaagattaa gtcgcttgat tgaagaaaac tcaggcaacg
     5521 actagcaagg agtgacaata tgttagcaca aatcaatcga gttgcttttg agcttgtagg
     5581 gattcctatc tactggtatg cattgatcat cgtatcaggt atcattatcg ctatgtggct
     5641 tagcagcaga gaggctgttc gggtaggatt aaaagcagat gatgtgacag attttatgtt
     5701 gtggggattg cctatctcca ttattggcgc tcgcttatat tatgttttat ttgatcttcc
     5761 ccagtatata gctgatccaa tccaaatatt caatatccgt tcaggaggct tagctattta
     5821 tggcggactg attgcaggcg cgatcacgct gtattttttt actaaatatc attttatttc
     5881 tttgtggact tttttagata tcgctgcacc aagtgtatta ttagcacagg caatcggtcg
     5941 ttggggaaat ttcatgaacc acgaagcata tggaccagac acgacgcgaa gcttcttaga
     6001 aagtctgcat ttaccgcaat tcatcattga taatatgtat atcgaaggga tctatcgtca
     6061 accaacattt ctttatgaat cactttggag tttgattgga tttattttgc ttttgttttt
     6121 acgaaagaaa ccacaccttt tgaaaaaagg cgaagtcctt ttatgttatc taatgtggta
     6181 tagtttcggg cgtttcttta ttgaaggatt acgtacagac agtctttact ttttaggtgt
     6241 gatccgtatt tctcaattat tatctgctgt gttatttgtc ggtacattga ttctatttat
     6301 ttggcgcaga aaaaaaggaa atttaccgga ttatgaccgc tcattaggaa aaaatcaaca
     6361 aattatctga tttttagagc gttaaaaatg aaaaaaattt caggagtggt aaaatgaatt
     6421 atagagtagc ggttttagga ccaggctctt ggggtactgc tttggcacaa gtcctcgccg
     6481 aaaacggaca tgacgttcgg atttggggac atcgtgcttc acaagtagaa gaaatcaaca
     6541 cccaacacac gaatcaacgt tatttaccag atcattatct acccgattcc atccaagcat
     6601 tcaccaatat ggaagatgca gtaaaagacg cagatgcggt gctattcgtc attccaacga
     6661 aagcaatacg ttctgttgca aaagaactag tgcctaaatt gaatacaaag ccagtgatca
     6721 tccatgcaag taaaggatta gaacagggaa cacataaaag gatctctgac atcttgttag
     6781 aggaaatccc agcagaaaaa agaaaagctg ttgttgttct ttctggacca agccatgcag
     6841 aagaagtagc aaaacatgac atcactacga tcacagctgc aagtacagat gcaaaagcag
     6901 caaaatatgt acaagagctg ttcatgaata attattttcg gatctatacc aatcctgatg
     6961 tgatcggtgt agagatgggg gcagctttga aaaatattat tgctattggt gctggcgcta
     7021 ttcacggctt agggtttggc gataatgcaa aggcagcaat catgacgcgc ggattagctg
     7081 agatcagccg tttaggtgta gcaatgggtg ccaacccgct gacctttatc ggattaagcg
     7141 gaataggcga tctagtcgtt acttgcacaa gtgttcattc taggaactgg cgcgcaggca
     7201 acttgttagg acaagggcag cccctagaag aggtgctagc caatatggga atggtcgttg
     7261 aaggtgtgaa tactacccaa gcagcgatgg aactagccca aatgatggaa atcgaaatgc
     7321 ccatcactca aacaatctat gaagtgctgt atgaacaaaa agaaatcaaa acagccgcaa
     7381 aagaaatcat gttacgtgat ggaaaaaaag aaaatgaatt ctcattaaca attttttgaa
     7441 ggagcgaatg tatatgaaag ttaaaaaagc agttattcca gcagcaggat taggaacaag
     7501 atttttacca gcaacaaaag ctatggcaaa ggaaatgttg ccaattgttg ataaaccaac
     7561 aatccagttc atcgttgaag aagcgttgaa atcaggtatc gaagacatct tgatcgttac
     7621 aggaaaagct aaacgaccaa tcgaagatca ctttgattca aatattgaat tagaaacaaa
     7681 cctaagtgaa aaaggaaaga ccgaattatt gaaattagta gaagaaacga cagatgtcaa
     7741 cttgcatttc atccgtcaat ctcatccaaa gggtttagga catgccgttc ttcaagcaag
     7801 agcctttatt ggaaatgaac catttgtcgt tatgctaggc gatgatataa tggaagacga
     7861 agtaccattg acacagcaat taatcgatga ttacgaacgt actcatgctt ctacgatcgc
     7921 cgttatgcaa gttcctcatg atgaaacttc aaaatacgga attatcaatc caggagaagt
     7981 attggaagat gggttataca atgtcaaaaa ctttgttgaa aaaccagatc caagcaaagc
     8041 accaagtgat ttagctatta ttggacgtta cttattgact ccagaaattt ttgatgtatt
     8101 gtccgagcaa caaccaggag ccggaaatga aatccaacta actgacgcaa tcgatacatt
     8161 gaataaaaca caacgcgtat ttgcccgtaa attcacaggt aaacgttacg atgtcggtga
     8221 caaatttggt tttatgaaaa caagtattga atatggtcta acacatccag aagtaggtgc
     8281 acctttacgt gagtacatcc tgtctttagg taaaacatta gaagcagaag gaagcaagat
     8341 cgatcataat ccaaaaggaa taacagaaat caaagaagaa aagaaaaaat aacccttttt
     8401 tctaacgagc ttcagctgct aattgaataa aaagtagttg aagctcgttt ttatttgttt
     8461 aaaaaacacg aaaatgctat ccttatagaa aagagagaca agaagggagt ttgatcatgt
     8521 caggaggaga aattgcagga ttgatcgcag caatcgcctt tgcgatactt gtcgttttta
     8581 tcgttcgtgt gttgttgcaa gtttctaaaa ctttagaaaa agtagatcag actgtagcag
     8641 aagcaaatac gacgatcgaa atcgtcacaa aagacgtaga tcttttgtct agacaagtag
     8701 aaggcttact ggtcaaaagt aatgaactgc tcttggatat caatggaaaa gtcgcaacga
     8761 tcgatccatt gttcacagca gttgctgatt taagtgaaag tgtatcagaa cttaatcatt
     8821 ctagtaaaaa catcgcgacg aaagtcggcg gaatcggtaa aacgacagct aaagcgacgg
     8881 ttgccggaaa agtcggtggg acagcgatga aattttttaa aaacaaaaaa catactgaaa
     8941 cgacaggagg aaaataaaaa tggctaaaaa aggtggattt ttattaggtg cagtagtcgg
     9001 aggcacagcc gcagcaatcg ccgcgttatt gttagcacca aaatctggta aagaacttcg
     9061 ggatgaatta gctgcccaag cggatgattt gatggatatg gcttcagatt acacagatat
     9121 ggcgaaagaa aaatcgactg agttaacagg tattgcaaaa gaaaaagcgc aagatctttc
     9181 tcaacaagca ggtgatctag caggtagtgt caaacaaaaa gtaagtgaat cagcagatga
     9241 agcaggaagt gaatctgacg atatcttgtt aaatcttaaa gaacaatcag ctgacttatc
     9301 taatcgtttc aaaaaaacgg cagaagatat gaaaaaacct gcagatgaat taggaagtat
     9361 tgctaaagat acttcagaag atatcatgat tgatgtaaaa gacgctgctg gtaaagcagg
     9421 agatactgtc aatgaaggaa tgtcagaagc aaaaccagtt gcagatgact caaaagaaat
     9481 gatgaaaatg gcaaaagaag atgcgaaaga agctaaaaaa gaaataaaag atacaatgac
     9541 agattcatct acaaatcact aaaatcacaa atttatagaa taaaaaggtg ggagcggact
     9601 agctctcacc ttttttactc ttatttttct aaaacaagta attcttttgg tgtagtcgtg
     9661 aatggcacac agccatcgct tgtcacatat acacagtctt cgatacgtac ccctactttt
     9721 tcaggaatat agattcctgg ttcaagagag aagcacatcc cttcttcgat tactaagtca
     9781 tttccttcta caagggaagg gaattcgtgg acggttgttc cgatcccgtg acctaaacgg
     9841 tgattgaaat attcgccgta gccatagctg ttgatcacgt ttcgtgcgat ttgatcaagt
     9901 tcacctgctg tcacacctgg acggacggct tcttgtgcgg caagctgtgc ttcaagagtg
     9961 atgttgtaga ttttttcttg gaagtcggtt ggtttttgat aagcaattgt acgagtagca
    10021 tcactgcaat agccattcca aacaacgcca agatcgaata acacaagttc attcggtgaa
    10081 atcttcgtat ttcctggtgt accatgaggg cttgcagcat ttgctcctgc aaggactaat
    10141 gtgtcgaaag acatttgtga aacaccttgt tttttcaatt ggtattcaat ttctgcaatg
    10201 atcgcttgtt cagtcacgcc ttctttcact gctttaaagc caatctcaaa tgcaacatcg
    10261 gcccatttac cagcttcgat cagtttgtct ttttcctcta gagttttgat cagttgtaga
    10321 cgttggatga tggaagttag atcagtgtaa gaatccgttc ccgtcaagat atcagagata
    10381 gcatcgaagc gctcgacagt caaggcgttc ttttcaatcc ctacattgcc aggctgacca
    10441 tagcgagcac gaagttcttt tccgatgatc tcccaagggt tttcgctgtc taaatagcca
    10501 cggacatcat acgcccaact actgttttga gcatcttcaa cttccaaagc aggagtgaaa
    10561 aggaaaggat cttgttcaag agggataaaa agagctagta cacgctcatg aggatcactt
    10621 tcgtatccgg aaaagtaagc gatgtggcca gggtcgctga tataagccaa ttcgacttgg
    10681 tgttgtgcca tccattgacg tagttcattg atttttacgt tattcatttg gcaacctact
    10741 ttctaaatta attttgtatt caataattgt accactgatt tattaaaaga ccaacgttct
    10801 aaaaactgaa aaaagtaaaa attttcatga aaacagagat aaaacggttg caattcttga
    10861 gattatttga tattctattt tagaaatgta agacgtattt tcagaaaaca tataaaatat
    10921 gaataggaga acacaaatgg aaaagcaaac aatcactatc tatgatgtag cgagagaggc
    10981 aaatgtttcg atggctactg tttcacgtgt cgtaaatggc aatccaaacg ttaaaccagc
    11041 aacaagaaaa aaagtcttgg aagttatcga tcgcttggat tatcgtccaa acgcagttgc
    11101 gcgtggcttg gcaagtaaga aaacaacaac agttggtgta atcatccctg atgtcagcaa
    11161 tatgttcttt gcttctttag cacgtggtat cgatgatgtg gcaacaatgt acaaatacaa
    11221 tattatctta gcaaattcag atggcaacga ccaaaaagaa gtcaatgtat tgaataactt
    11281 actggctaag caagtggacg gcgtgatctt catgggtcac catatcacag acgaaatccg
    11341 tggagaattt tcacgttcaa aaactcctgt tgtattagct ggctctattg atccagatga
    11401 acaagtaggt agcgtaaaca ttgattatgt atccgcaaca aaagatgcag taaacaaatt
    11461 ggcaaaaagc ggcaataaaa aaatcgcttt tgtcagtggt gcgttgatcg atcccatcaa
    11521 tggacaaaat cgtctgaaag gctataaaga agcattaagc gaaaataacc taccatatag
    11581 cgaaggattg atctttgaag cacaatacaa cttcaaagat ggtctatctc tagtcgatcg
    11641 gatccataat agcggtgcaa cagctgtcta tgtttcagat gatgaattag ccatcggtat
    11701 tttggatggt ttattagacc gtggcgtgaa agtaccggaa gaattcgaaa tcatcacaag
    11761 taacaattca ctactgacag aagtagctcg tcctcgttta acaagtatca cacaaccatt
    11821 atacgattta ggggcggtat ctatgcgcct attgacgaaa atgatgaata aagaagaagt
    11881 cgaagaaaaa acgattatct tgccttatgg aatcgaagaa aaaggatcta caaaataata
    11941 aaaggacaac cattcagagg ttgtgtgcct gccgtttaac tctgcgttac aaggagcaat
    12001 ttcaaaaaac agcatctcat gtttgatgaa tattgtgact cgtgattcgg agttggcagg
    12061 agaacaccgt ttatttggga aaaggggtta tgacaaaatt ttgttataat cctttttttg
    12121 tgtcttaaaa aagagagaaa gtggttgaaa ttacatttat cgtatataaa acaatccgtg
    12181 tatagatgct gcattttgtt caccaccatc aaaaaaataa gggactgtga caatactatt
    12241 gtcacaatcc cttattactg tgtattctat taaatcaatc atctttctca tctgattctt
    12301 tagttgcttg attcgctctt gcataagctg aagcggtgtt ccagtaatct gtatagtttt
    12361 catcggtacc gccaacgcca aatttaaagg cgcttttctt cggtccattt ttcgcccata
    12421 gcgattcaac tgtttttcct ggtgtttgga tggatttttt attgacagta acttttccag
    12481 gtgtttgacc agtaaatcca gatacttttt tcttcatcac atcagatgat aattcgaatt
    12541 tctcatcagt gccaaaaatg tcaggttttg cttggtaagc ttgattgatc agattggcaa
    12601 gataattagc cgaacgtatc cttgctttgc tgtccattcc agtattgtca tcgtgccctg
    12661 cccagctact gatggtaata gacggagtag agacaattag ccatgaatct cggtattcat
    12721 tggtagaacc agtttttccg acccaatctg cttttcctag atttccgttt aagcttgaga
    12781 tggcatcttt gaatggtgta gtgatttttg cattgatcac actacgcatc atgtcattca
    12841 tgatcgatgc cgttgcttcc gaatagatcc ggacagagtt gctttcgtgt ttataaatta
    12901 catttcctgc gttatctgtg atggaatcga tgatatagcc ttcttcatag acgccgccgt
    12961 ttgctaaagc ctgaaatccg ttggtctgtt gaagcgtcgt gacatctact gtacctaaag
    13021 gagcagattc aacgccccaa ttgtcatttg ccgggtagtt catctttgcc aagtagtgct
    13081 cgtaagcata ttgtttcgat ccaccggaat tcaacacatc ttgatataag tgataagccg
    13141 ggatattgtt ggaccattct agtgactcac ggacggtttg gaaggtgttc gatccttcgt
    13201 tagtagcatt gacgattttc tcgccagcat tttctccttc ttgccaagtt gttgcataat
    13261 ctgatacacg ggattctgat ccgataagcc ccatgtcgat tgcaggtcca taaacaagaa
    13321 ctggtttgat tgatgagcca gcttgacgtt ctgtatcgaa ggcatggtta ttttgattct
    13381 gactgtaatt ccgtccgcca acgaatccgt agatgcggcc agttctgtta tccatcaata
    13441 catttccggt ttctacttga ctgccagcac catcatctaa catgtatcca taattttcaa
    13501 cgccattttg caatgcagca tagacatctt tatcgattgt cgaatggatt gtatatcctt
    13561 tattttggat cgtttgctgt gccttttgat aataagcgtc actgaccgat gaatcagaca
    13621 tatcagcgtt gtctttttca gccaattgtt gtgcaatgat tcttgttgct tcattcatca
    13681 cagtataata caagaattct ctgtcagtct gttctgcgat ttgttgtggt agaaagtctt
    13741 ttgtaaggtc ataggctttt gcttcttcgt attgttcttt cgtaatttgg ccttcacgat
    13801 acatactgaa taaaacaaaa tcttttctgg caaggccggc agaaagatct tcttttagcg
    13861 cgcctgtgtt cgtgtatgga ctatacgtga tcgggctttg gggtaatcca gcgatataag
    13921 cagcttgcgg cagcgtcaca tcttttgcat ctacaccaaa gattcctttt gcagcttcct
    13981 ggactccagc aatgttttgt cccttattat tacgaccaaa tggtgagaca ttcaaatacg
    14041 tagcaatgat ctcatctttt gaaaagtatt tttctacttg tgcagaaaga atgatctcgt
    14101 ttgctttacg tttgaatgtt gtttcatctg taaggatctg ctgtttgact aattgctgtg
    14161 tcaaagttga tccaccagaa gagccaatac cagtagcttc agatactaat gctcgtaaaa
    14221 cagctttagg aacgtaacct tggtgtttat cgaagtattc atcctctgtg ctgataattg
    14281 cagttttcaa taaaggtgaa atcttatcag atgaaatagt cgtgcgcatc agatctgtct
    14341 ggatttttga tatctctgta ttatctgcat aaacaatctt tgacgtttct gtaatgtttc
    14401 ctagttctgt ctgcagtgct tttttagtcg gcaattgtgt gtcttcgacg agataggcaa
    14461 aatagccagc gcctattcca atacctagag ctgcgaacat caaaagaaga acagtaataa
    14521 aaacagtcaa tgattgaaaa actctaaaaa taatcttagg aacaaaccat ttttcttttt
    14581 ttcttctatt ctgttttttt cgagtatttt tgcgtggcaa aagggtcacc tcaactaatg
    14641 gttttatttc gctaattata gcaaataata gaacaaaaaa acagacaagg ctgtttttgg
    14701 caggaatttc ttttttaaaa gagttaaaac gtgagaaaat gatctttatt ataaaagagt
    14761 tcttaagctt ttctttagag aaaatttcag ttactatgga gaacagggca aagaaaaatc
    14821 aatcattttc tttgtatatc atcttaccaa tatataggaa ttgttagtaa caattgcctt
    14881 tcaaggagga atccatccat gttattaggc tcacacgtca gcatgagcgg aaagaaaatg
    14941 ctgttaggag cagcagaaga agcagccagt tatggatctt cgacttttat gatctataca
    15001 ggcgcccctc aaaacactag aagaaaagcc atcgaagaga tgaatattga tgccggaaaa
    15061 gcgtacatgg cagaaaaagg tctaacaaat atcgtcgttc atgcccctta tatcattaat
    15121 ttggggaata cgatcaaacc agaaatgttt ccttttgccg tccaatttct gagagaagag
    15181 attgttcgtg cggaagcttt aggcgctact caaattacac ttcacccagg tgctcatgta
    15241 ggggcaggaa cagaagccgg aattgctaga attattgaag gcttgaatga agttttgaca
    15301 aaagaacaga cggcacaaat cgctttggag acaatggcag gtaaaggtac ggaaatcggt
    15361 cgaacatttg aggaattagc gcaaatcatc gatggagtaa aattgaatga aaaattatcc
    15421 gttacttttg acacctgtca tacgaatgat gccggttata atattaaaga agacttttca
    15481 ggtgtaatgg aagagtttga taagatcatc gggttagatc ggcttaaagt actccatatc
    15541 aatgattcta aaaatccgca aggttcgcat aaagaccggc acgccaatat tggttttgga
    15601 acaatcggat tcgacacgct gaataagatc gtccatgatc caatgtttga caatatttcg
    15661 aaaatcttgg aaactcccta tgtaggcaaa gataaaaaga aagcttacgc accatatgga
    15721 aaagaaatcg ccatgttcaa agcgcaaaca ttcgatccag aagtgtttcc tgagttatta
    15781 gcagaagaag tgatactcta agattttcta tctgacagca gcaaaaaagg accaggcaca
    15841 ttgccttgtc cttttttgtt taagaaacaa aggagtactt tttgcagtct ttttcatcat
    15901 gcatcttctc ccccctcttt ttaaatttag acaaacaagg tatactggta gaaaagtaaa
    15961 aaaacgaaaa gtttcacaag caagtggcaa aacgtgattg gaaggacgaa aagatgctga
    16021 acactttaaa acccttgatc aaccaactaa aaaagaaaca tacatacttt attcttggca
    16081 tagccctatt atttacaatt attcgtttgt atttatatag taaagcaatc tatggaacag
    16141 acatgtcggc aacctatgat gaccagctgc tgattcgcta tgctcaaaat attttggacg
    16201 gtaaatggct aggggagtat acgactacaa cactttccaa aggaatctcc tacagcttat
    16261 ttttagtact tgcacataaa ctgcatcttt cttatgcagt tttgttcggc atgttcagta
    16321 tttttgccag tatattggtt gcactcgcgt ttaaaccgat tgtgaagaac aagtatcttc
    16381 ttttaagtat ctatttttat tttttgtttt ctccgattag ttttacacat gaatattcca
    16441 ctcgaacata tcgtaatgcc ctcgtgattc cggctgttga gatcattatc gcttgtttat
    16501 tagctattta ttaccgcaaa tacggttcta gcagaaaatt gctgccatgg tttttagggc
    16561 tttctgtcgc ttttcctttc ttttggttca ttcgcgaaga ttctatctgg cttcttcctt
    16621 ttactgtagt agctttgggt atcagtttcg tccagatctt gctagggcaa gacttttctt
    16681 tcaaagaagt aaagaagtta aaaaaacgac tctttcttct ctcgaaacgg gaatggctga
    16741 aactttcatt gtttttactt cctatagttg gcttttccat cgtctatcaa tggatcgaat
    16801 ataaaaatga gacaacatac ggtattgcaa ccatcaatga ccgttccagt ggagaatttg
    16861 gacgtttgat gaaacaactg atccgaatag atgatgaaac gaacttgaat aaagaaaaca
    16921 gtgagatatg ggtctcacat gctgcactaa ataaagcatt agccgcatct cctacattcc
    16981 atactgtttc tacccgtatt gacgaattat atacgactca cgcgtggact caaggtggaa
    17041 aagtaaaaga attacccgga gatatcatct tttgggcatt gcgagatgtg gtaagcgaat
    17101 ctggttatta ccaaaacaat gcaaaagaaa ccaatgattt ttggaaaaaa gtcaaccaag
    17161 aattagaaga agcctatcac aagggaacaa tcaaaaagaa aaaagagttc tatctgatgg
    17221 gatcaggaga tggaaagacg atgagtgatc tgccaatcgt attcgatttc cttcaatcag
    17281 gatacgctta tgcatggcga tataaagatt tccatcaagg aggaaattat agtttcggaa
    17341 cgcaagaaca agtcaaacag gctcaagaat tattaaacct tccgctaatg gagaattgga
    17401 tcgatagtaa tgaaccgaga acacctaatt tccaattaac aaaatcagca aaagtcgcga
    17461 atgtcatcat aaaaatttac caagttctgg ctattcccgt gctgattgct tcaatagcag
    17521 ggatattact tatgatacta ggaaccgtat ttgcaaaagc tgatcgtgcc tattatagtt
    17581 cctctttgat tgtggtcacc ggattaatac tgacacaatt ggtttttctt ttcggtgtct
    17641 catggttttg ttcttatgcg ccagaaaaaa aggactattt tttaagtgtc tacactggtg
    17701 caggcgtgcc attaacgcaa ttcatcgtag taacaagcat gctagttttc agtaaattaa
    17761 atctgaaaaa agctttatct tctaaataaa aagacccttg gtagctagat ttttaaccta
    17821 actatcaagg gtctttgtgt ggaaaaatca gtttttattc gatttcttct accgtttcct
    17881 gcaaaagctg ttcatatttt ttgtttcctt tatataaatc accaatcgtc catgccaaca
    17941 ggagaaggac aagaacaatg attacataag cgatgtgatc agccaatgct agttgagcgg
    18001 aagtggcttg ctcgccgaag aaactttcga tctgtcctgg taatcctcgt aagttcaagt
    18061 aagttagact aatcacagag aaccagccaa gaagttttac gatgaatgaa tttttaaatc
    18121 ggctacccat ctccgcttgg ctgttggtca tcatcagtaa aggaatcatc gaaaacggca
    18181 acgcaaaagc caagaacacc tgggattcat tcattaagtt attcaaagcg gcatgttctt
    18241 cgatcgttcc ttttgtactt gtaaacaaaa cacaaatcaa tacaggaatc acagaaagca
    18301 atcgtgtgac cagtcttctt agccaaatcg ggatacgcat atggatgaat ccttccatga
    18361 tgacttgacc ggtcaacgta ccagtaatag tagagttctg gccagaagca agaagtgcta
    18421 cagcaaataa agtggacaag ataccagatt ttgctacagc aatcaaaata ccattgctca
    18481 tagctgatgg atcagataaa gcttcataca gaccaaaaaa ggaaggatct tggacagaac
    18541 cggttttgaa gacagcgaca cccatgatca atagcagtgc attgactaga aaagccattg
    18601 acaactggat attagaatcc caagtagaaa aacgtacagc ttgtgctaca tcttcttcgt
    18661 tttggtgatc gatcttccga gtttgtgaaa ctgctgaatg aagatataag ttatgcggca
    18721 tgactgttgc accaataatc cctaaagctc cagtcagcgg agtttcgccg ccgatgaccg
    18781 gtgagtctga gaatgtatga ctgttaggga taaagccagc aaataaactg tgccaatctg
    18841 ggttagacaa gccaacttga tagacaaaaa cgaataaaat gacgaaaatt aagcagacaa
    18901 ccagtgcttc gatcttacga aacccgactt ttgtcagtag taagagaacg aaaacatcta
    18961 atacagtaat aaagacggca atcaccaatg gaatatcaaa aagtaagtag agggcgattg
    19021 ctgcaccaat gacttctgca atatctgttg ccataatggc taattcagtc aagatccata
    19081 aaatgacacc taatgtctta cttgtgcgtg ctcggattgc ctgtgccaaa tccatttggc
    19141 tcacgattcc taattttgct gccatatact gtaaaagcat agcaatcaga ctagacatca
    19201 aaataactga catcaataaa tattggaaat tttgtcctcc ggtgatcgaa gtagaccagt
    19261 tacccgggtc catataccca actgctacta acgcaccagg tccagagtaa gcaagaagtg
    19321 ttttaaaaaa acttttcccc tttggtacat caactgttcc atttacttca gctaaagata
    19381 aaccatttgt atgttcaata agtcgatgct tatgaaattg tttattggat tgattattca
    19441 agaaaaaacc acctttccta tttatctacg attacattct acacctcaaa aagaaaaaat
    19501 taaagaaaaa gattcggtac gcctaatttt ttttcatggc gcgtcttgct agatgacgaa
    19561 agaaattttg ttctcctcta aaaaaaccac tattttttgc atgaaactaa agaatacgcc
    19621 tttctttttt caataaactt tagtaaaata agtcattgag agcaattcgg aaatagaaag
    19681 gagagattta tatgttatca acaggtattg tcgtacttat tgctattatt gctttgctaa
    19741 tcggcgcagt aggtggcttt ttcctagcgc gtaaatatat gaaagactat ttagaaaaaa
    19801 atcctcccgt taatgaggac atgttgcgat caatgatgat gtcaatgggg caaaaaccat
    19861 ctgagaagaa aattcgccag atgatgcagc aaatgaagaa ccaagggaaa aaataggttc
    19921 tgacccaacg actttttacg ggctgatttt ttcagtccgt tttttatttc gtaatttgtt
    19981 agactattca gattattcag caaaaaagac aagcacagac aatcctaggc tcttgataaa
    20041 gacaaaggag gagtaaaatg tcgattttta gaaaattagg ctggtttttt cgtcaggaaa
    20101 agaaacatta tttaattggg gttttctcgc tattcgcagt ggccttggta cagttagttc
    20161 ctccaaaagt gattgggatc atcattgatg agatcgccga taaaaattta tcaataaaga
    20221 tcattttcgg gtggatcttc ctattgatta tcgctgcttt tttacaatat ctttttcgtt
    20281 atatctggcg gacgaatatt tggggaagtg cagctcgatt ggagaaagaa cttcggcagc
    20341 agttattcaa acattttaca gaaatggaca atctctttta ccaaaaatat cggactgggg
    20401 atttgatggc tcacgcaacg aatgatttga atgcgatcca gaatgtagca ggagctggta
    20461 tcttgacctt tgcagattca gtgattacgg gcggaacaac gatcattgcg atgattttgt
    20521 ttattgattg gcgcttaaca ctgattgcgc tgattcctct gcctttgctg gccgtcactt
    20581 cacgagtgct ggggtcaaaa ctccatgatg cttttcgtga ttcgcaagaa gctttttcta
    20641 gtatcaatga taagacacaa gaaagtatca ctgggatcaa agtcattaaa acatttggac
    20701 aagaaaaaga agatctagcg gattttgaag aaaagattga agatgcaatt attaaaaaca
    20761 aacgagtcaa ttttttagac tccttatttg atccattcat taccttgatt atcggtctgt
    20821 cttatgttgc aacaattatt ttaggtggaa agtatgtctt ggaaggcacg atctcgatcg
    20881 ggcagatggt ctcatttatt agttatatcg gtatgcttgt ttggccgatg tttgcgattg
    20941 gtcgattgtt caacgtattg gaaagaggaa atgcttctta tgatcgcgtc aatgaacttt
    21001 tgcatgaaaa gacgcatatc gtggaacaac tgggtgcagt gcagaccccg gcaaaaggcg
    21061 agctgaatgt agctatttcc tcttttaatt atccgaaaga agaatcacca gcattagaac
    21121 aaatcaagtt tgcgatccaa gaaggagaaa cattaggtat tgttgggaaa actggtgcgg
    21181 gaaaaacgac gatcatcaaa ttactgatgc gtgaatacga tgattatcaa gggaaaatcc
    21241 aatttggcgg acgttcgatc aaagaatatt cattgaatgc tttattagga tcaatcggct
    21301 atgtgcctca ggatcacttc ttgttttcga tgaccattcg agataatatc cgtttttcta
    21361 atccaaaact ttcagaagaa gaagtagagc atgcggcttc tttagcattt atcaacaatg
    21421 aaatcaagga attaccaaaa ggttatgata ccttagttgg agaacgtgga gtctctttat
    21481 ctggcggaca gaaacaaagg atttcgattg ctcgagcatt gatcaaaaat cctgaattat
    21541 taatattaga tgatgcactc tctgcagtag atgccaaaac agaagaagct atcttgcata
    21601 atttaaaaga aataagaaaa gagaaaacaa ctatcattgc agctcaccgt ttaagtagcg
    21661 tgatgcatgc aaaagaaatc attgttgtcg atcaagggaa aatcattgaa cgaggaaccc
    21721 atcatgagct gcttgcacta ggcggctggt atgaacaaat gtgggagaaa cagcaattag
    21781 aagcgaagat agaaggaggc gaggcctgat ggatgaaaat tatcaatcgg aatggacaaa
    21841 atcgattcca cttaaagaac agaaaaagat catcaaacga ctgatgaagt tcgccaaacc
    21901 ttttagaaga acgttcatcg ttgctatctt atttgcgttt gctttgtctg tgatcaatgt
    21961 cctattgcca aggatcatcc aaacttttat ggatgatcat ctggctaaac agtcggctac
    22021 gacacaagtt attttgtttt ttgctggtat gtacttattt ggagtcatag taaaaagtat
    22081 catttggttt ttccaatggt atttgtattc catggcctca ttgaagacct atcagtatat
    22141 tagggtaaaa ttatttgaaa agctgcatac actaggtatg cgttattttg atcagacacc
    22201 ggcaggatca accgtttctc gagtgacgaa tgacacagaa acgctatttg aattttggta
    22261 cgtctttttg atggtattga ctgggatctt tgcagtcgtt tcttcattcc ttgccatgtt
    22321 tcaaatcaat ggagaaattg ccttgtggac tttggtgttt ttgccgattt taggaatcgt
    22381 catctggtat taccagaaat tcagttctcg tatttaccga aatatgcggg aaaaattaag
    22441 tcagctgaac acgaaattga atgaatcgat ttccggcatg cgtattatcc agcagtttcg
    22501 ccaagaagct cgtttagcta aagaatttga acaagtgaac aatgagtatt tagacacgcg
    22561 ttatgcgatg atcaaaacga attcgattct gttaagtccg atcatcaatt ttctatatgc
    22621 attggcgatt gcgttggtgt tgactttgtt tgggatcgat gcgttgcatt ctcccgtaga
    22681 agttggactg atctatgcat ttacgaccta tgtccaggga ttttttaatc caatgtctca
    22741 aatgatggac tttttgagca tcttcacaga tgggatggtt gctggtagtc gtatcttgaa
    22801 gattatggat acggaagaat atgcaccaca acaaaaccct ggagcagatc aaatgattac
    22861 ttcaggtaag attgagtttc gcaatgtaag cttttcttat gatggagagc acaatgtatt
    22921 aaacaatatc tcttttgttg ctaatccggg agagaccgtt gcattagtgg gacacaccgg
    22981 aagcggaaaa agctcgatca tcaacgtatt gatgcggttt tacgaatttt atgaaggtca
    23041 gatattgatt gatgatcatg acattcgaga atatccaatg gaagagttac gtaagaaaat
    23101 ggggctggtc ttgcaagatg ctttcttgtt ttatggggat atcgcaggaa atattcggat
    23161 gatgaatcca gctatcacag acgaacagat acgccatgcg gctgagtttg ttcaagcaaa
    23221 caaattcatc gatgaattac caaaaggata tcactcaaaa gtgatcgaac atggtgccag
    23281 ctattctagt ggacaacgac agctgatcac ttttgcgcga acaatcgtga ctgatcctaa
    23341 aattctagta ttggatgaag caacagcaaa tatcgatact gaaacagaag gattgatcca
    23401 agaaggattg gcaaaaatgc gtcaaggacg gacaacgatt gcgattgccc atcggctgtc
    23461 aacgattcga gatgcagaat tgattttagt tttagacaaa ggtcatatag tagaacgcgg
    23521 gaatcatgaa acacttctag cgcaaaatgg tttgtatgca gatatgtaca aactgcaaag
    23581 cagtggtata taaaagatgt tgtgacagta gaattttgac ctaagcacta gagtgaaaat
    23641 aaaacatagc ttctcatatt tttcatctgt gagagttagt gtcgtagtca ccagatttaa
    23701 aaaaagattc gacagctagt cagaagtgcc taatagccac taagaaagcg ttgacaaaag
    23761 catttgcttt cgataatata aagaaaacga tacaacttgg atagtgattg cctgatgcaa
    23821 gctaaacctg atgagtctcc catggtttca tggacggctt tttgggtttt tctttttgtt
    23881 ttggatacag ccctatacat catggaaagg agcaaaacat gctgattgag aaagtactga
    23941 ataacaacgt cgtaataacc agggatgaaa gaaatcagga aatgatcgtg atgggaaaag
    24001 gtttggcatt caaaaagaaa gtgggtgacc cgatttttga ctcatgcatc gacaaaaaat
    24061 ttcagttgac aaatgacggg ctttcacaac agttccaaga attggcacaa gcgattcctc
    24121 ttgagtactt agaaatttct tgtgaaatca ttgaatatac aaaagaggct ctacaagtcc
    24181 agttgaatga ttctgtgtat attgcattga cggatcattt gtatacggct atcgaacgtc
    24241 acaaaatggg aatatttgta ccaaatgtat tgctgtatga tatcaaacaa ctatttccta
    24301 aagagtatgc catcgggaaa aaaacagtga agaaaatctc ggataaatac cacatcaagt
    24361 tatcagaaga tgaagccgga tttatcgcat tgcatttagt caatgcacaa ggcattcaaa
    24421 gccagcaaga caatacctac ctgtttacta taacaataca ggatattcta aatatcgtcc
    24481 gctattacta taagattaca tttgatgaag attcagtcca tttttttcga ttcgtcacac
    24541 acttacgttt ttttctgaca cgcgtcctag atggagaaaa atcagaagtg ggagaagttt
    24601 ctgaggaatt gtttcaggtt attcaaaaaa aatataagga tacgtttggt tgcgttgaga
    24661 aaatagcaga ctttttagcc gagacgttca actaccagat gtccacggac gaaaaaatgt
    24721 atttaacgat ccatactgcg cgtttagtgc atgagaatcg ttgtaaataa aaaaaggagg
    24781 ataaattaac aagaacgtat gaaataaggg tagtgacatt cagttggcta aaccttccac
    24841 taataaattg gagggaatag aaatgggaaa ataccaagaa ttagcgagaa aaatcgtaga
    24901 aaatgtcggc gggaaggaaa atatcaatgg tttgacacat tgtatcacaa ggcttcgttt
    24961 caaattaaaa aacgaagaaa aagcaaatga tgagatcttg aaaaatatgg acggtattgt
    25021 aacggtaatg cgagcgggag ggcagtatca agttgtcatt gggaatcatg tgccagtcgt
    25081 gtttgaagag gttataaaag caggaaacct tacttttgat gaggctgttt caacagaaaa
    25141 aatgagaccg tttgatatgc ttattgatgg aatatcgggc tgtttccagc cattccttgc
    25201 tatcctagct gctggcggga tgattcgtgg actgacagca ttccttgtct tcttaggcgc
    25261 gtttgaccgc ggttctggca catttgttat gtttgataat atcggtgatt ctgtattcca
    25321 atttatgcct gttattatcg gtttaacagc tgcccgtaaa tttaaagtaa atgaattcgt
    25381 agggatgttg cttggcgcag cattaatgaa tcctagcttg tcgctggaag ctttgagtgg
    25441 tgcagcagaa gcaccactaa cgaccatatt ttctggcact atttttgaag cacctattta
    25501 tcaaacagta ttcggcattc cctggattgc gcgtaactac gcttcttctg tcattcccat
    25561 tatctttatt gtgttgttag ctagccaaat tcagaaacca attaaaaagt tagtacctga
    25621 gatgatcgcg aatttcttcg ttccattctt caccgttctt attacaatgc ctttaggttt
    25681 tttactcgtt ggaccagtat tcacattcgc aactgatatt ttaatggctg gatttgaaac
    25741 acttttagca ctttcgccag ttatttatgg agcaatcgta ggcttcttct ggcaaattct
    25801 ggtgatgttt ggtctccatt gggcaatcgt accaatggga ttgatgcaat tttctgtgaa
    25861 tggttggcaa aatattatga cacctgttgc agtagtgtct tttggacaaa ctgcagcact
    25921 taccgcactc tactttaagc ttagaaatcc aaaagacaaa gcaattgcaa taccagccat
    25981 tgtttctggt attgtgggga ttacagagcc agctatttat gggtttacat taccacgtaa
    26041 aaaaatattt atttttacct gtgtcggtgg tgcgttaggg ggcgcgtata gtgggttaat
    26101 gaatttaact tcttggaatc aaggtggact aggcatcttt acaattccca actatattag
    26161 accagatggt gatttaacag atgtgattaa tgtattgatt gggatcgcca ttgcgatggt
    26221 ggtatccttt acgctgactt ttttcttttg gaaggatgaa gcaggtgaaa cagatgatat
    26281 acagaaaaaa tcaggtaaag aaattgtaaa aacaccgata caaggacaaa tcgctccact
    26341 aaatgcagca aaggatgcag cttttgcaca aggaacacta ggtcgaggaa tcttgatcta
    26401 ccctgaaaaa ggagaagtac gtgccccttt tgacggaaca gtcatgaccc tgttcccgac
    26461 gaaacaggca attggaatgg tttctgaaac agggctggaa ttgctgattc atgtcggatt
    26521 ggatactgtg cagttggaag ggaaatattt tgaaagctta gtgcagcaag gataaagagt
    26581 gaaacaaggc gatctgttgc tacgcttcga caaaaagaaa atcgaaaagg aaggatacag
    26641 tttagaaaca cctgtgatcg tcaccaatta cacggattat ttggatattt tggaaaacca
    26701 aggggagacg gtcgggccag acgatgcact tatcaccgca ctgacctgaa cggttaggtg
    26761 aagaatggac taggcttaac tattcttttt attccacttc tttattttca aaattgaaag
    26821 gctctttcat gatggctgag ctgatagggc gtttatcgaa tttttgaccg acagaaatta
    26881 gatggactaa attctgttga taattttctg caaaaacaca actatataac acgtctagaa
    26941 gataacaaat tgatgtattg atcgtaaaat taccaatttt tgaatacagt cgctcgcgtg
    27001 tcgtaagtcg caaagcggct gtactcattg cagatagcgt tgaatcaccg atactggtca
    27061 aagctaaaat tggaacatga ttttgtttca atatttctgc ggtacgaagg atcacattgt
    27121 tttcacccgt ataagaaatc agaatcccgc acatatcact cgtgcaattg tacgcttcat
    27181 agaatgattc accaaaagtg gtgacaatcg tgacatccat tttgatgcgc ttcattttca
    27241 gcatgaaatc ttgagaaatc aaagtattcg agttactggc gaaaatcacg attttttttg
    27301 cttgaagcat tagttgtttg gctttttgca gttggtcatg atgaatgaga gaaagtgtat
    27361 catgaatcgt catctgttct aaagaagcca ttttatttgc gatcgtcata ataccgtctg
    27421 tggtggaaaa aggaagattc gcatctacgt cttgaaaatt cccttcaaga taggctgttt
    27481 cagctaaaaa agcattacga aaactgaccc agccatcaaa acccattttt tttgctacac
    27541 ggatgagtgt agaggggtgg acataacatc gctcagcaat cgcttggatg gtcatttcgc
    27601 caagtgctgg agaatcgctt aagagaaact ggatcacttc attttctgaa ggagaaaagg
    27661 agatttcttt gattttttca cgtaataaca tgtagatacc ttcttcttat ttttttacgt
    27721 aaattataac ttttttgaaa atgatttgta aacggattta tactatataa cttaaaatag
    27781 acattttttg acaagatatt tacaaaaatc cactagaaaa cagtaaattt tgtaaatgtt
    27841 actggaaaga aacagatttc tattgtttgt tttgtttaca aaaaagataa tgaaagtgta
    27901 aaaaagtaaa ggagtgagaa catggcaaaa ggaatcaaaa tcgctacgat tggtggaggc
    27961 agcagttaca cccccgagtt gatggaagga tttatcaaaa gatatgagga attaccgata
    28021 cgagaaatct ggctcgtaga tgtagaagaa ggaaaagaaa agcttgagat cgttagtcag
    28081 atgtctcagc gtatgtggga tgcttctccg tatgaagtca agatctatgc aactctggat
    28141 cgggaagaag cattgaaaga tgcggatttt gttacgactc agtttcgtgt cggtcaatta
    28201 gatgcacgaa tcaaagacga gcggatccct agttattatg ggatgattgg gcaagaaaca
    28261 aatggtgctg gcgggatatt caaagctttt cgtaccgttc cagtcgtttt ggcaatcgtt
    28321 gaggacatga agcgtttatg tccaaatgct tggctgatca atttcgcgaa cccaagtggg
    28381 atggtgacag aagcagttat cagatatggc aaatgggata aagtgattgg attatgcaat
    28441 gtgccagtga tggcgatgat gacagaacca gcaatgcttg gcaagaaaaa agaagagctg
    28501 atctacaagt ttgccggtct aaatcatttc cattggcaca aagtagcaga taataaagga
    28561 aatgaccgca cggcagaatt gattgatcgt ttatacgatg aagagaatgg attaccgaaa
    28621 aatatctttg atatcccgtt tttcaaagaa caattaaaac aaatgaacat gatcccttgt
    28681 ggctaccacc gctattacta tcgtgaagaa gaaatgctgg aacatgcttt agaagaatat
    28741 caaacgattg gtacaagagc gcagcaagtg aaagaaacag aagcagagct atttgaatta
    28801 tacaaagatc cagaattaga ccataaacct gaacaattgc aaaaacgagg tggcgcttat
    28861 tattcagacg ctgcctgtga aacgattgct tcaatctatg gaaataaaga aacacagatc
    28921 gtggtatcta caaagaatga tggagctgtt cctgacctgc cggcagattg tgtagtagaa
    28981 gtcactgcct atgttggcgc acaaggagcg agaaatgtag cgtttggttc tcttcctgct
    29041 gctgaaagag gctggttgca agtaatgaag aatatggaat tgctgacgat cgaagcagct
    29101 gtgactggtg attatgggac agctttacag gcgtttacga tcaatccact tatacgatca
    29161 ggaaagacag caaaacggat catggatgag ttgtttattg cacaccagca gtatttaccg
    29221 caattcaagg agacaatcag ccgattaaaa gaagaaggaa tcgaagttca agatgaactg
    29281 gcaagagaat tatcaggtaa tacacatata atggaggaag tataatggga ttcagaaaag
    29341 attttttatg gggcggtgct acagcagcca atcaatgtga gggcggttat gatgaaggtg
    29401 gacgcggact agcaaacgta gatgttgtgc caatcggaaa agaccgtttt cctgttatca
    29461 ccggaaagaa aaaaatgttt gaatttgatg aggatcattt ttatccagca aaaattgcaa
    29521 tcgatatgta tcatcgttat aaagaagata ttgctttgtt tgcagaaatg gggttcaaga
    29581 cttatcgaat gtccatcgcc tggagtcgta tttttccaat gggggatgag aaagagccga
    29641 atgaagctgg cttgaaattt tatgaggatg cctttaaaga atgccaaaag cacggaattg
    29701 agccacttgt gacgattacg cattttgatt gtccaatgca tttgatcaaa gaatatggtg
    29761 cgtggcgttc cagagaactc gttggttttt atgaaaatct gtgtcgagtc atcttcaatc
    29821 gctataaagg acttgtcaaa tattggctga cattcaatga gatcaatatg atccttcatg
    29881 caccattcat gggcgcaggg ctgtattttg aaaaaggaga aaatgaagaa caaatcaaat
    29941 atcaagcggc acatcatgag ttggttgcaa gtgccatcgc aacgaagatt gctcatgaag
    30001 tagatccgga gaacaaagta ggctgtatgc tggcagcagg agcatattat ccatacactt
    30061 gcaatcctga agatgtttgg gcttctagaa aatcagatcg agaaaattat ttcttcattg
    30121 atgtccagtc acgaggagaa tatcccgctt atgcattgaa agaaatggag cgaaatggca
    30181 ttcagatcga aatggaacca ggggatgccg cattgctgaa agaacatact gttgatttta
    30241 tctctttttc ttattatgct tcccgagttt cgactaccga caacgaactc ttggaacaga
    30301 ctgcaggaaa tatctttgct tctgtcaaaa atccatatct gcaagcaagt gaatgggggt
    30361 ggcaaattga tccattaggc ttgagaataa cgatgaatga tatttatgat cgttatcaaa
    30421 aaccattgtt tattgtagaa aacggcttag gtgcagtcga tacgccagat gaaaatggtt
    30481 atgtggaaga tgattaccga atcgattacc tggcagcgca tatccaggcg atgaaggatg
    30541 cagtagaact agacggagtc gatctattag gctatacgac ttggggatgt attgatttag
    30601 tatcagctgg taccggtgag atgaagaaaa ggtacggatt catttatgtc gatcgtgata
    30661 accaaggaaa tggcacatta gcccgttcaa agaaaaaatc attcaactgg tataaaaaag
    30721 taattgattc aaatggagaa aatctttcga acgattgatc aatgatttat aaaagtctat
    30781 tgtttttcag cggatttttc gagtataata aagctattcg agaaacgcaa gcagaacaat
    30841 agactttttt tcgcattggc tgtaaagtat gcttacttga ttaagtaaag gagaatctac
    30901 atatgtatga tgtaatcgtt attggcgcag gtcctgctgg gatgactgct gctttatatg
    30961 cttcacgttc aaacttatcc gtattgatga tcgaacaagg tgcccctggc ggccaaatga
    31021 acaatacggc agaaatcgaa aattatcctg gattcgattc gatcatgggc cccgatctag
    31081 cttataagat gtatgaaagt gtctcaagat ttgggacaga aaatgcttat ggaatcgttc
    31141 aagatatcaa ggatcacggc agccataaag aagtcatctg tgaagataaa agttacgaag
    31201 gaaaaacagt cattatcgca acaggatgta tccaccgtaa attaggcgta cctggcgagg
    31261 aagaatttgc tggacgaggc gtttcttact gtgcagtctg tgatggtgcg tttttccgta
    31321 acaaacgtct agtagtcatc gggggtgggg actcagcagt agaagaagcc atctatttga
    31381 cacaatttgc ttctgaagta gtgatcgtcc accgtagaga tgaattacgt gcacaaaaga
    31441 tcatccaaga ccgtgcattt gcaaatgaaa aaatttcctt tatgtgggat acagtcgttg
    31501 acgaaatcgt tggaaacgaa atggtagtga caggtgtgaa agcacgtaat gtcaaaaccg
    31561 gcaatgaatt tgatttagca gcagacggag tattcatcta tgttggactg gatccattaa
    31621 ctgcaccttt tagagaagca ggtttgacga acgaagctgg ctggattcca acagacaacg
    31681 aaatgaaaac gaatatgcca ggcgtttttg ccatcggaga tgttcgtgaa aaagatttgc
    31741 gacaaatcac tacagctgtt ggtgacggcg gtattgctgg acaacaagtg tataaatact
    31801 tggagtcttt agaagaaaca acagcataag tttaaaaaga gtagaagaac gggttcaatt
    31861 gttcttttac tctttttttt cgcgtgaaat ttactgaatc tgatttatgc gaacacgtcc
    31921 aacacctttt gataaaagaa tcctttcttg atttaagcgt tttttggtcc tcatactttc
    31981 atatctaata agtgaatgct atacttatta agaaccatat ttcagaaaga ggtgttattt
    32041 tcatgtcttg ggaacaagtg tatgaacaat gggtacatga agaaaattta gaagaaaact
    32101 taaaaaaaca attaaatgat ctgggtgaag atcctgaaaa acttgaagat gctttttatg
    32161 caccgttgga attcggtact gcaggtatgc ggggaatctt aggacctggg atcaatcgaa
    32221 tgaacatcta tacggtaaga caagcaacag aaggactagc acgtttcatg gatacacaag
    32281 gtgaagaaac aaaacgccga ggtgtcgcta tcgcttatga ctcacgccat cagtcaccag
    32341 aatttgcaat ggaagcagcc aaaacactag cacatcacaa tatcccgtct tatgtattcg
    32401 aaagtctgcg tccaacacca gagctttctt ttgcagttcg tcatttgaag acgtttgcgg
    32461 gaatcatggt aaccgcatct cacaacccag cggcttacaa tggatataaa gtctacggag
    32521 aagatggcgg acaaatgcct cctgcagacg cagatgcatt gacgaaatat gtccgtgaag
    32581 tcagcaatcc gctaaaagtc gaagtattat ctgacgaaga agctaaacat agcgggttga
    32641 tcacgatcat cggagaagaa gtggacgctg cttatctaga agaaatcaaa gcagtgacga
    32701 ttgatcgtga attggttgaa acaatgggga aagatttgaa acttgtgtac acgccacttc
    32761 atggaacagg gaaaatgttg ggcgaacgtg cgttgaaaca agctggattc gaacaattcg
    32821 tactagttcc cgaacaagct gtggcagatc cagatttcac tacggtcaaa tcgccaaatc
    32881 cagaagaaca ttcagctttt gaatatgcta tacgacttgg agaaaaagaa ggagctgact
    32941 tgctgattgc aacagatcct gatgcagacc gtttaggcgc agcggttcgt ttgccagatg
    33001 gttcttatga agtattgact gggaaccaaa tcggagcatt gtttgctcaa tatattttgg
    33061 aagcacatca atcaagaggc actttaccag aaaacgcagc ggtattgaaa tcaatcgttt
    33121 ccagcgaatt gcctacagca attgcggaac actacggtgc taccatgttc aatgtgttga
    33181 cagggtttaa attcattgct gaaaaaatcc aacaatttga agaagaccat tcacatactt
    33241 ttatgtttgg attcgaagaa agttatggtt atttagttaa accttttgtt cgcgataaag
    33301 acgcgatcca agcattgctt ttattagcag aagtagcagc ttactataaa aaacaaggca
    33361 aaacgttgta tgacggcttg caaacgattt ttgaaaccta tggctatttc gcagaaaaaa
    33421 cgatttctgt gacaatgagc ggacaagaag gttctgcgaa aatctctgct ttgatggaaa
    33481 aattccgtaa agaagcaccg acagaatttg ccggagcaaa agttattcaa acagaagact
    33541 ttaaagaatt gacgagaaca gaccgtgaag gaaacgtaga aaaactaaca acaccgccat
    33601 ccgacgtatt gaagtataca ttggaagaaa atggctggat cgctatccgc ccttcaggaa
    33661 ctgagccgaa aatcaagttt tatattggtg taaaaggaag ctctacagaa gacgcagccg
    33721 aaaaagtagc ctcttatgaa gcgattatca aaaagatgac agaagaataa aaaacttgag
    33781 actgatggaa aaagtggtaa actttagaag tcagtctttt ttcaagttgg aggtgaatgg
    33841 attgccagac acgcaaaaat caaaagtaga gaaagtcagt caattattca aaatgttgag
    33901 tgatcccact cgtcttaaaa ttctgctata tctgaaagac ggggaacaaa atgtgacagc
    33961 aatcacccaa gcagtggaaa tggagcagtc tgctgtatcg catcaactac gtttattaag
    34021 agaaaatcat gtcgtcaagt ctcatcgcga gggaaaagcg attttgtata gtcttgatga
    34081 ttctcatgta ctggatattt tgaaccagac actcaaacat gtggaacatc aataacgata
    34141 ggtgaaaata gataaacact ggagtgtgac aacgaagaaa tttcgttgtc acactccagt
    34201 gttttttaag aaaaagacaa aaatgattaa tcgctttttt ctgttgtttc agttccataa
    34261 taactatgat cttctaggtc taaagcattc aaaataagcg aagccgtttc ttcaatagat
    34321 aaagatgcca cattgatcac tacacatcct agtttttcat aaagatcatt cgcaaaatcg
    34381 agttcagctc taattttttc gatatcggaa taagcagtat cagggtttaa tccgtaagag
    34441 cgcatccgtt ctttacggat attatttaaa atatctgggt tattcgttag tcccacgatt
    34501 tttttaggat cgatttccca cagctgtttg ggaatatgtg cttggggaat caaaggtaaa
    34561 ttagcaactt ttaaattttt atttgctaaa aacaagctca aaggcgtttt ggaagttcgg
    34621 gatacgccta gcaaaacgac atctgcttct aaaaaaccac gaggatcttt cccatcatcg
    34681 tattttacag caaattccat tgctttgatc cgtttgaaat agttctcatt caataaatgg
    34741 agggcaccag gttgccgaat tggcttgata ccagttaaac gttcaatttc tgatatcggt
    34801 ggcgtcatca catcgaaact aaataattga tgttcttcac aaaaagaacg tgttatctcg
    34861 atcagttctt gattgattaa tgtatgcagg acaagtgctt tttcttctaa tgcatcttgc
    34921 aatgcctgcc ttaatgtttt ttcatctgtc acgaacgttc tgcggaatag gttgaactca
    34981 acggtaggat actgagccat agtagcttgc gctagttttg tcgcagtttc tccagcagag
    35041 tcggagatga caaaaaaagt aacgatttct gtagaatcgg aagacatgga taaaacctca
    35101 cttgcatttt ttttatgttt attataccat actctagaat gcgtacttcg taatcatttt
    35161 gatttggagg aataagtcat gaaaaaacag acattgatcg aacaatcatt agaaacattg
    35221 aaaaataacg gttttaaata tactaaaaaa agagaagcat tgttaactta tttagtgaag
    35281 cgtaaccgct acgtatctgc taaagaagtt tttgactata tgaatcagga attcaaaggg
    35341 gtaagttatg atacgattta ccggaatctc catgattttg cagagatcga tttactggaa
    35401 gaaaccgatt taaatggtga aatgaagttt cgttttcatt gctgtcaggg ggaaatcggc
    35461 catcaccatc attttatctg tacagtgtgc ggaacaacga aagaaatcca tatgtgtcca
    35521 atgaattatt tcgaagaaca gctaggtgga tgcacgatcg aagggcatag attcgagatt
    35581 ttcggaagat gtgaaaattg tcaataagga tttaaataaa ataacatttg ataatttaaa
    35641 aaaaaagcaa gatttccttg acggtcaaaa cagcattaga tataatattt acaggacagt
    35701 cttgtttttt gagatatatt tcaataacaa ttacggttac tttttaagct cggagggagg
    35761 gaattaacat gtcaaaaaca gtcgttcgca aaaacgaatc acttgacgat gctcttcgtc
    35821 gcttcaaacg ttccgtgtca aaagcgggta ctttacaaga atctcgtaaa cgtgaattct
    35881 acgaaaaacc aagtgtaaga cgcaagaaaa aatctgaagc agctagaaaa cgcaaaaaat
    35941 tctagttctg tgatgaattg gagttggtag tgtgtcacta ctaagtagat taaacgatga
    36001 catgaaagca gcgatgaaag cgaaagataa ggaatcttta caagtcatcc gaatgatcaa
    36061 gtcatctata caaaacgagc agattaaaga aggtcacgac ttgacagaag aagaagaact
    36121 aacggtgttg tctcgcgaaa tgaaacagcg acgtgattct ttgcatgagt ttgaagaagc
    36181 aggacgtgac gatttagctg aaaaagtaaa aagtgaaatt gtaatagtcg aaaaatatat
    36241 gccggaacaa ctttccgacg aagaaattcg acaacttgtt caagaagcaa tcactcaaac
    36301 tggtgcttca tcaatgaaag aatttggcaa agttatgggt gcaattatgc ctaaagtcaa
    36361 aggaaaagca gatggaaacc aagtgaatgc gattgtgaaa gaactattac aatcttaatt
    36421 ttatcattta aactgcggac agtgattgtc cgcagttttt tgtgttcgta aaaaaagaac
    36481 taaaaaacag ctagaagaaa gaataagatt ctttcttcta gctgtttttt tcctgtttaa
    36541 tatctcttat gttaaattgt gtttgatttg gttattcttc tggtagttca tttgcttctt
    36601 gtttataccc tgataggcga taggcgtaat aaaagagtaa gctgccagcg taaccaagta
    36661 acgttgggac catccagcca agtcctaatg cagcaaaagg aacgtgatga tgataaaatc
    36721 ctagtaagct tgctacgaca gtttgctgac tcactaatgc aggcgaattt gccaacatat
    36781 ccaatacggc tggaacggca gaaaagaaca tcgttacttg atagacaaat ggactctttt
    36841 ggaagaattt tgctgttaag gaaagcaaga ttaacgcaag tgacaatgga tacaaaagca
    36901 tcaatactgg taaagaccat tggatgatat tatccaatcc ggcattcgca acgacaaaag
    36961 agacaaaagt cgttaaccgc agccatgtca tataactgac tttagggaac agtttatgga
    37021 aatcttgtgc aaaagaaaca acgagtccca tagcggtagt gaagacacct aatgtgacca
    37081 ttacccctaa gaagctagca ccgaagttac ctaaataaaa attagtgatc tgagaaagtg
    37141 cagttccgcc attttctgat aaaggcatct tattcaaact catcgcaccg agaaggacca
    37201 gaccaaaata aatcatgact tctaacgcaa cgctcaggct accagctttt gctgttaatt
    37261 ctgtgacttt tttatcttta aaaccaagat cacgaactgc atggacaaaa gtgacactta
    37321 aagcaagtaa agctactgca tcaaccgtgt tgtatccttc taaaacgcca tttgtcaaag
    37381 cgttcgtctg ataagaatct gcagcagatt ggtttagatt ccccatgggt tggaataaag
    37441 aaacgacaaa aatgatagct aataatgcaa gaaacaagga gtttaaatac tttccgacat
    37501 atttgaccaa acgtgttgga tgtacagtaa ggaaataagc aagtaaaaag aaaagagcag
    37561 agaataaaaa catacctaat gaatcatatt tttcaggcaa aaagggttta gcagccattt
    37621 caaaagcagt tgctgctgtc cgcggtgtac caaagaatgg tccgatcgtt aaatgaacaa
    37681 ggatcaagaa caaggcagca taccattttc cgacaggttt agctaagtca tacagaccat
    37741 cactttttgt cactactaca gctaagatag ctaataatgg gaaaagagcg cctgagatgg
    37801 caaatcctac accagcgctg atccagtttg atccagccat ttgacctaag tggacaggaa
    37861 agatcagatt acctgacccg aagaacatcc cgaagataag tgaactgacc atcaataatt
    37921 gcttccatga ataagttttt gtttcttcca taaatttttc ctccaataat tatcattttg
    37981 agtaacaaaa taaacgttgc agaaactaga aatgccacta atatcagatt ccatgaaaac
    38041 caccacctta aaatataaaa aaaagccctt ttccacttgg gaaaagggct ctagattcta
    38101 gaacggtacc accttttttc gcttaaaaaa gcttcttcat tcacggacgc ttttcgataa
    38161 agttatccgc tagcctgata atggaggcag ccacaatgat ctacgttttc gacattgact
    38221 cggaaatgat gttcgtctat tcgatttatt gtcatttctc acttacatga ctcactggga
    38281 ataaatcgct gaatagccta ctgttttcct cgacgctttg ctgtatattt atttgttgac
    38341 gttataatac acctgttttt tttgaaatgc aagaatattt caaatatttt gttttctttt
    38401 tttgaaacaa gaaaaatacc cagagaatac ttgctaagtt agacggaata cttgtcctct
    38461 atcttatgca tagcttacat gatcagcttt ttttattcaa acgagacctt tagagaatgg
    38521 aagcagctat tttttaacgt ttcttggtac ttatggttaa gcaactcttt tagaaccttt
    38581 tcttatttgc tatatagttt cttctgagta tggtaaaata ggaaagaatg aatcgaaaaa
    38641 aaggggatgt ctatttttga cggaagagtc tagttcttta gagattagat tgacggctgc
    38701 tgatgatgtc agcatgcttt taggtgcaca cgacaagcat ataaaactga ttgaagaaac
    38761 aacagaaaca accatccata cacgtggaga aatcattcag atcattggcg aacaatccca
    38821 gatttccttg gctgcctcag tcattcggac attgcaggaa ttgatcaaac gaggaatcca
    38881 tgtaagtaca ccagatgtcg tgacggcact gaaaatgggg caaaaaggca cattagatta
    38941 ttttgttgaa atgtatgagg aagagatcgt caaagaccgt aatggaaaac caatacgggt
    39001 aaaaaacagc ggccaaaaga aatatgtcga atcggtagcc aaacatgata tcgtatttgg
    39061 cgtaggacca gccgggacag gaaaaacttt tcttgcagta gtattagcaa tcgctgcctt
    39121 gaaaaaaggt gaagtgcaaa aaatcatttt gacgcggcct gctgtggaag caggggaaaa
    39181 tctaggcttt cttcccgggg atctaaaaga aaaagtagat ccttatctgc gtcctgttta
    39241 tgatgcttta taccaaattt ttggattaga tcatacgaac cgattgatgg aacgaggcgt
    39301 gatcgaaatt gctcctttag cttacatgcg ggggcggaca ttggatgatg cattcgttat
    39361 tttggatgaa gcacagaaca cgacagttgc tcagatgaaa atgttcctga cacgtcttgg
    39421 ttatcaatca aaaatgattg tcaatggaga tacgagtcag attgatctgc ctcgagggac
    39481 aacaagtggt cttgttcatg cagaacgaac attaaagcaa atcaagaaaa tcgattttgt
    39541 taattttgaa gcaagtgacg ttgttcggca ccctgtagtt gccgaaatca tcaaagctta
    39601 tgaaaaggca gatctccatc aagaataagc tttgcttagg aggataagca catgttgaat
    39661 aaaacactgc agaaactaca agaaaaactt ggaaaggcag cattgccttt cctgctgttc
    39721 ttgtttttta tcattatctg cggcgttaca tttagtagtg tcaaacaaaa ggccaatgat
    39781 ttcagagaag gccaagtagc agaagaaagt atccgtgcga acaaaacgat cgagaacacg
    39841 gaagaaaccg aacaaaaaag aaaactggca gcagaagcag tcactccaga atacacttac
    39901 caaaaagatc ttgcagatga ccagaacaat cggatcaagc aactatttga attgatcgat
    39961 aaaacgaatg atgccatcaa caaatcttat gacgaaaaga ttgataaggc aaaggaagat
    40021 gaaaatatcc caaagcctac tacagaagag cggatcgccg cgttgaagaa aaattttgaa
    40081 aatgtgaatg cagaaaatgt tgcgttttat caaaaactac cgaacaattt ttattcgacg
    40141 atatttgaaa tgactgaaac tgagatccat actgtgaggg atgagagtct gaagctcatt
    40201 gacgaacaga tgagcaaaca agttcgtgaa agtgaattgg aagcttttaa acaagaagca
    40261 gaagagcaaa tccaatattt gaatgtcaca ccagcacaac aacaaatgat tcgctactta
    40321 gtggatcaag gtatcgtagt gaatgacgta ttgaatgaga aaaagacaga agaattgaaa
    40381 caatccgcac gagaagctgt acaaccagtg atgatcttcc aaggagaaat cattgttcgt
    40441 gaaggtaatc aaatcgatgc ttcagctatg aaaaaattgg aattactagg actgactaat
    40501 caaacaactt cgatttttcc gctaattgcc atggttttag ctgttttgct tcaaatcggc
    40561 gtactgattt ataattcact tcaattccaa aatgcaggag aaagaacaaa atacgtgctt
    40621 ttctatgtga cagctatgtc agtcagcata gtgctgatga aatttttcca attattccaa
    40681 acggaacaag ctacttatat cccgctgttt tatcccgctg cttttgcacc acttgtttta
    40741 agctttttct tgaaccgaag agcagggatc atggctggta ttttccaagc agtatctgca
    40801 cttttcattt tttatgattc gattggcacg aactttctaa cgattatctt gatgtcttat
    40861 gttttctctg ggctcatggc aacgattgtc agacgcaaac ggatctcaga acaaggatta
    40921 tctgctgcca tgtgggtcat tatttttcct gtgatgatgg atgtcatttt gatcgtctat
    40981 caaggaatga gctttggtga tgcaacgact tggttaatgc ttgtctgcgg gttcactggt
    41041 gcactcttct cattcttgct gacgatcggt ctacacccat atattgaatt gatggtcaat
    41101 gatgacagtg taattgtatt aaatgaactc agcaatccaa atcatccttt attgaaacag
    41161 ttgctggaag aagcgccagg tacttatcat catagtatga tggtagctaa tctaagtgct
    41221 aacgctgtag cagaaatcgg cggacgttct ttgctaactc gagtagcttg ctattatcat
    41281 gatatcggca agatcaaaca tgctagcttt ttcgttgaga acttgccttc aggagcggaa
    41341 aacccgcaca atttcttatt gccagaagac agtaaacata ttatctttgg acatgtgaca
    41401 gatggagcca aaatattgga agattataat atgccgcaaa tggtgatcga tatttgcaaa
    41461 cagcatcatg gaaccacgct aatgcgttat ttctacatca aagcgaaaga acgcaatccg
    41521 gaagtaacag aagagcaatt ccgttatcct ggaccaaaac cgcaaagtcg tgaagcaggg
    41581 atcgtcaata ttgctgacag ttgtgaggct gcagtacggg caatggatca tccgacgagc
    41641 gaaaaaatcg aaagttttgt ccataatctt atcgaagagc gtatttccga tggtcaatta
    41701 gacgatagtg gactgacctt gaaagaaatc cgaaaagtgg aaaaatcatt gatcagcggt
    41761 ttaagtagca ccttccattc tcggatcaaa tatcctaaaa tgaaaacaga agttgagaag
    41821 ataaaggaag aacaagaaga atagaaagaa gcgatgtata atggatatta cctttatgga
    41881 tgaaacaaac caagtctcag aagagaaaat gaaagaaatc gatgacttgc tgcaatttgc
    41941 agcatcttat ttaaagctgc cagaggaaac agaaatgtct gttactttca tggacaatgc
    42001 ggcaatccaa gtaatcaatc gtgattaccg tggaaaagat gcaccgacag acgtcatcag
    42061 ttttgctctg gaagaagaag gcgaagacga aatcccagtg atttttgaag atggagagaa
    42121 tccattgcct cgtgtactag gagacatcat gatttctatt gaacgtgctg ctgaacaagc
    42181 agaagcttac ggccattcat atgatcggga acttggcttt ttagcggtac atggattctt
    42241 gcatatcaac gggtatgacc atatgacacc agaagatgag aaagaaatgt ttggtttaca
    42301 gaaagagatc ttggatgcct atggactcaa aagataaaaa cgtagagaaa aacaaacatt
    42361 tcctgatgtc tgtggaattt gctgtccaag gagtcaaaac cgtttttgag gaagaacgca
    42421 atatgaggaa acatgttgcg ttcggtatcc ttgctttatt tatgggcgtg attttccagc
    42481 ttgatcggat agagtggtta tggctgcttt tagctgtttt tctcgtttgg atagtagaga
    42541 tcatcaatac tgtttttgaa aatgtagtgg acatgtttac ggattttcat tttcatccga
    42601 ttgggaaaaa aatcaaagat atggcagctg gagcagtttt agtgaccgct tgttttgctg
    42661 tcatcattgg gttgatttta tttgtcccta aaatatatca tttgttgttt aattaaaaag
    42721 gagaacgtat atgccagaac ataaatcagg gtttgtcgca atcgtcggcc gcccgaatgt
    42781 aggaaaatcc acattattga accgaatcgt gggtcaaaaa attgccatta tgagtgacaa
    42841 ggcacagaca actagaaata aaatccaagg agtatatact acaccagaag ctcagctgat
    42901 ttttatcgat acaccaggaa tccataaacc aaagcatcgt ctaggagatt ttatggtaga
    42961 agcagcttac agtgcgttga aagaagtgga tgcagtctta tttatgatca gtgctgatca
    43021 aaaacgcggc cggggagacg attttattat tgaacgtcta aaaaacgtgc attctccagt
    43081 ttatctgatt atcaataaaa tcgataagat ccatccagac gagttgctgg gaatcatcga
    43141 agattattcg acgcagatgc cttttgcaca agtgattccg atctctgcta cagaaggaaa
    43201 caatgtcgaa cgcttaatgg atgtattagt cagtgaaatg ccagagggcc cacaatattt
    43261 tccagatgat caagtgactg atcatcctga atactttatc gtttctgaat tgatccgtga
    43321 gaaagtcttg tttttaacac gagacgaagt gcctcattct gtcgcagtcg ttgttgattc
    43381 gatgaagcga aacgagaatg ataaaatcca tatccaggcc acgatcatcg tcgaaagaga
    43441 tagccaaaaa ggaatcatta tcggcaaagg cggaaaaatg ctgaagcaag taggaacgaa
    43501 agcaagaaag gatatcgaaa atcttttagg agacaaagta tttctagaac tttgggtcaa
    43561 agtccaaaaa gactggcgag acaaaaaagt ctatctgcaa gactttggct accgaaaaga
    43621 cgaatactga tattctgaac ttcttatagg ttaagtatag aaataaactg gataaaaatc
    43681 cgcacgttga gaaaaacggc ggaagatcga aacataaata atctggatcg gcagaatctg
    43741 cttttccaga tttatttttt aggagggaga atatgagcca gcttgcagaa tcgaaaggct
    43801 tgattttatc tagccgtaat tacaaagaaa aagataaatt agtcaaaatc tttacggaat
    43861 cttctggcaa aatgatgttt tatatcaaag gaatccatcg gcaaaatcag ccattagctc
    43921 cggctttgct cccctttacc gaagcagtct atatcggtaa ttttcgaagt gaaggtttgt
    43981 cgtttttaag cagtgtcaaa gacgtccatg cgtttcggaa catccaagaa gatatctttt
    44041 tgagcggtta tggtacctat ttgctaaatc tagtagatgc agcaatcgaa gatcatcaat
    44101 atgacccgca tttattccaa tttacgcaac aagctctgca gcggatggat caaggagatg
    44161 atgcagaaat catcacgaat atctttgaag ttcaaatact gcaacgcttc ggaatcgcgc
    44221 caatttggac gcactgtgtc gtatgcgggg aaacaaaagg aaaatttgac tattcttcca
    44281 aatctggcgg tgtgatatgc gagaaacact ggccaatgga cgaacaccgt tatcatgcag
    44341 atcctcgcgc tgtttatttc gttcgaatgt tttcggctat ttcttatgat aagatcagtg
    44401 gaatccactt gaaagaagaa acgaagcagg caatccgtga cctcattgac cagttgtatg
    44461 atgaatatgt cggtattcat ctaaaaagca aaaagttcat tgatcaaatg aagtcttggg
    44521 cacacgtatt aaagacgcca gagaaaaata tgcctgaaaa agagatacct gaaaaagaag
    44581 aataaatgaa agatacaaca aaaaaatcaa aactatttga caaatacagg gacttccgtc
    44641 tattattaac cgtagattaa atagatcgtt cactgagagg atctattata taaacaagaa
    44701 aactgcaaaa tgaatacgta cattgaccaa gatcagtaaa aaacacgtgg tttagcgagt
    44761 tcgggagagt ggaagccgga cccaaacgat ttttgaagag gcacttgcga gtacgataaa
    44821 tgaagctgcc gaatctttat tcggaagtag ggtggaaccg cgaaattatt cgtccctatg
    44881 tctgtaatca gacataggga ctttttgtcg ttttctttcc tgtattctta ttgacagcaa
    44941 cgcttaaaaa ttgaaagggg caaaacagat gagtaaaaaa ttgaccgtcc aagatatgat
    45001 tttaacatta caaaaatttt ggtcatcaaa cggctgcatg ctgatgcaag cttatgacac
    45061 agaaaaagga gcaggtacga tgagcccata tacattctta cgggcaatcg gaccggaacc
    45121 atggaatgct gcttatgtag agccatcccg ccgaccagct gacgggcgtt atggagaaaa
    45181 cccaaaccgt ttgtatcagc atcatcaatt tcaagtcgta atgaaacctt cacctgaaaa
    45241 tatccaagag ctttatttag aaagcttgcg tcttttagga attgatccat tagaacacga
    45301 tattcgtttt gttgaagaca actgggagaa cccatcgatg gggtgtgccg gtgtgggttg
    45361 ggaagtatgg cttgatggga tggagatcac acaattcacg tacttccagc aagtcggagg
    45421 actaccttgt aaaccagtta cttctgagat cacttatgga ctagaacgtc ttgcttcata
    45481 tattcaagaa gttgaaagtg tctatgatct ggaatgggct gatggtgtga aatatggcga
    45541 gatcttcaaa caaccagaat atgaacattc caaatattct tttgaagtaa gtgaccaaga
    45601 cttgctacta agcaactttg accgttttga aaaagaagct aaacgctgta tcgatgaaaa
    45661 tcttgttcat ccagcgtatg attatatttt gaaatgcagc catacattca atttattaga
    45721 tgctcgcggt gcagtatccg taacagaacg agcaggttat ctttcccgta tccgcaacat
    45781 ggctcgtgct gtagccaaaa tctttgtggc agaacgcgaa aaattaggat tccctttatt
    45841 gaataaaaac acagcaaatc cagaaaacat gaaggaggct gaataagatg gcaaaagact
    45901 tattacttga aatcgggctg gaagaaatgc ctgctcatgt tgtcacacca agcatgaaac
    45961 aattagaaga aaaaacagca aaatttttag atgaaaacca attgacttat gatacgatcg
    46021 aaacgttttc tacaccgcgc cgcttagcaa tcaaagtaac aaacattcca gaacgtcaag
    46081 cagacagtga agaagaagtc aaaggaccag ccaaaaaaat tgctttggac gcagatggaa
    46141 actggacgaa agcagctgaa ggatttgtcc gcggacaagg tctgacgaca gatgctatca
    46201 cttttagaga attaaaaggc gtggaatatg tctatgtaac gaaatacact cacggcaaag
    46261 aagcgaaaga agtattgaca ggtttagcag atgtcattaa aagcctcact ttcccagtaa
    46321 cgatgcattg ggcagaatac gattttgaat atatccgccc tattcactgg atcgtggcat
    46381 tgttagatga cgaagtaatc ccgtttgaga tcttagatgt caaaacaggc agagcctctc
    46441 gaggacatcg tttcttaggc gaagatgtga catttgcgca tgcaaaagaa tatgaagaaa
    46501 aattgaatga acaattcgtt attgcttcac cgcaaaaaag acaagaaatg atcgttacac
    46561 aagcaaatga attagccgaa aaaaatcact gggttattga tctagacgaa gatttactag
    46621 aagaagtcaa caatttagtc gaatatccaa ctgtcttttc tggcgacttc gaagaaaaat
    46681 atctttctgt tcctgaagaa gtactagtca cttctatgaa agaacatcaa cgttatttcg
    46741 aggtacggaa cgatcaaggt atgctgcttc ctcattttat ttctgctcgt aatggtgatc
    46801 gggtaaaatt ggaaaatgtg gttcgaggca atcaaaaagt attgacggct cgtttagaag
    46861 atgcggaatt cttttataat gaagacaaga aagcttcaat cgaccaatac gttgaaaaac
    46921 taaaaactgt tactttccat gaaaaaatcg gtagtatcta tgagaaaatg cagcgggtac
    46981 aagtcattgc ccgtatcatt ggcgaacaag taggactatc tgaggaagaa ttgatggatc
    47041 tgcaacgagc tgctgaaatc tataaatttg atctagtaac caacatggta ggggaatttc
    47101 ctgaactaca agggatcatg ggcgaaaaat atgctctgct gaaaggcgag aaacctgctg
    47161 tagcaacagc gattcgtgag cattacatgc ctatatccag tgaaggagag ctacctcagt
    47221 ctacagtagg ttctgtttta gcgattgcag ataaattaga tagcattttt tctttctttg
    47281 cagttgggat gattccaagc gggtcaaacg atccttatgc cttacgtcgc caagcatacg
    47341 gtgtcgttcg gatcatcgaa tcgaaaaaat gggcctttcc tttgtctgtt ctgcaagaaa
    47401 cgattagtga tgttatcagt aaagatacgg atcgtttcgg cattggttta tcagaagggc
    47461 aacaacaggt gatcgacttt atcaaaggcc gtctgcgcca attattgaca acgaagaata
    47521 ttcgtcatga tgtcatcgaa gctgttttga atgcagaaca aaaagatctg acaaaagtat
    47581 ttgcagcagc acaattgttc aagcagcatc ttgcagatga agacttcaaa ccatcgatgg
    47641 aagctttgac acgtgtcgtg aacttagcga aaaaagcgga gcttgaaaaa caatcagaag
    47701 tagaccctga actgttcgaa aatgaagcgg agaaagaact gcacaaagca gtagaggcaa
    47761 tgaaaaaaag ctttgctgac caaacgatca acgaaaatta tgatcagctt gtaagccttc
    47821 gcccactgat cgaaaattat tttgatcaga cgatggtcat ggcagaagaa ccagcagtac
    47881 gtaagaaccg attaacgcaa ttaagtcaaa ttgctgccat ggctttgtcg ctagcaagtt
    47941 tagatcaaat catcacaaaa taatttctaa tatgtaaata aaccagagaa taggcacaaa
//
LOCUS       DARBEC010000009        47952 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.009, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000009 DARBEC010000000
VERSION     DARBEC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47952)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47952)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47952
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.009"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..1189)
                     /locus_tag="SCL80_000490"
     CDS             complement(38..1189)
                     /locus_tag="SCL80_000490"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEG1106382.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(1202..1639)
                     /locus_tag="SCL80_000491"
     CDS             complement(1202..1639)
                     /locus_tag="SCL80_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1106383.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            complement(1654..3420)
                     /locus_tag="SCL80_000492"
     CDS             complement(1654..3420)
                     /locus_tag="SCL80_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEG1106384.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEALATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            3684..4361
                     /locus_tag="SCL80_000493"
     CDS             3684..4361
                     /locus_tag="SCL80_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106385.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            4364..5491
                     /locus_tag="SCL80_000494"
     CDS             4364..5491
                     /locus_tag="SCL80_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106386.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLPLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLPDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(5691..6131)
                     /locus_tag="SCL80_000495"
     CDS             complement(5691..6131)
                     /locus_tag="SCL80_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1106387.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAKTIEEVQMIFEHGHI"
     gene            complement(6142..8175)
                     /locus_tag="SCL80_000496"
     CDS             complement(6142..8175)
                     /locus_tag="SCL80_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1106388.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            complement(8177..9880)
                     /locus_tag="SCL80_000497"
     CDS             complement(8177..9880)
                     /locus_tag="SCL80_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1106389.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKKSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCL80_000498"
     CDS             complement(9974..10924)
                     /gene="manA"
                     /locus_tag="SCL80_000498"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEG1106390.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIATEPGK"
     gene            complement(10963..11988)
                     /locus_tag="SCL80_000499"
     CDS             complement(10963..11988)
                     /locus_tag="SCL80_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1106391.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            complement(12197..13066)
                     /locus_tag="SCL80_000500"
     CDS             complement(12197..13066)
                     /locus_tag="SCL80_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extradiol dioxygenase"
                     /protein_id="HEG1106392.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMIDFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSYFEKIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLTEASGGAIDL
                     PTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAIGIEWWFVSE
                     PKEKNKTDIRCDA"
     gene            complement(13525..15417)
                     /locus_tag="SCL80_000501"
     CDS             complement(13525..15417)
                     /locus_tag="SCL80_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106393.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(15414..17141)
                     /locus_tag="SCL80_000502"
     CDS             complement(15414..17141)
                     /locus_tag="SCL80_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106394.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(17157..17807)
                     /locus_tag="SCL80_000503"
     CDS             complement(17157..17807)
                     /locus_tag="SCL80_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1106395.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            18011..18307
                     /locus_tag="SCL80_000504"
     CDS             18011..18307
                     /locus_tag="SCL80_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1106396.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            18479..19099
                     /locus_tag="SCL80_000505"
     CDS             18479..19099
                     /locus_tag="SCL80_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEG1106397.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(19191..20357)
                     /locus_tag="SCL80_000506"
     CDS             complement(19191..20357)
                     /locus_tag="SCL80_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1106398.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(20369..20722)
                     /locus_tag="SCL80_000507"
     CDS             complement(20369..20722)
                     /locus_tag="SCL80_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1106399.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLETVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            20850..21872
                     /locus_tag="SCL80_000508"
     CDS             20850..21872
                     /locus_tag="SCL80_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEG1106400.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            21891..22052
                     /locus_tag="SCL80_000509"
     CDS             21891..22052
                     /locus_tag="SCL80_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106401.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(22311..22745)
                     /locus_tag="SCL80_000510"
     CDS             complement(22311..22745)
                     /locus_tag="SCL80_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106402.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLKKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(22855..24714)
                     /locus_tag="SCL80_000511"
     CDS             complement(22855..24714)
                     /locus_tag="SCL80_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1106403.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(24832..25992)
                     /locus_tag="SCL80_000512"
     CDS             complement(24832..25992)
                     /locus_tag="SCL80_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEG1106404.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCL80_000513"
     CDS             complement(26299..27723)
                     /gene="araA"
                     /locus_tag="SCL80_000513"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEG1106405.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(27739..28437)
                     /locus_tag="SCL80_000514"
     CDS             complement(27739..28437)
                     /locus_tag="SCL80_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1106406.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(28394..30055)
                     /locus_tag="SCL80_000515"
     CDS             complement(28394..30055)
                     /locus_tag="SCL80_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEG1106407.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKR
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            30273..31673
                     /locus_tag="SCL80_000516"
     CDS             30273..31673
                     /locus_tag="SCL80_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEG1106408.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKVLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIIGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(31925..33004)
                     /locus_tag="SCL80_000517"
     CDS             complement(31925..33004)
                     /locus_tag="SCL80_000517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1106409.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(33090..34049)
                     /locus_tag="SCL80_000518"
     CDS             complement(33090..34049)
                     /locus_tag="SCL80_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1106410.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(34061..35572)
                     /locus_tag="SCL80_000519"
     CDS             complement(34061..35572)
                     /locus_tag="SCL80_000519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1106411.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(35594..36157)
                     /locus_tag="SCL80_000520"
     CDS             complement(35594..36157)
                     /locus_tag="SCL80_000520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
                     /protein_id="HEG1106412.1"
                     /translation="MKIVETKFYGQLIVVSDYILLGILWVIVCLPVITIVPATCAVFY
                     VMAQWKGEGSGNIIYFFFQGIKQHFGKNLFLSLLTIIIYFLVNQLLEEQTIVLSISGY
                     VVLLIYSMFLLSWIYVISENKDITFLQIFEQSAWKVFFDFLRNILCCVLLLGFVLLIF
                     LFPPFIFIFAGGVWKLVSIILTIGNER"
     gene            complement(36163..37026)
                     /locus_tag="SCL80_000521"
     CDS             complement(36163..37026)
                     /locus_tag="SCL80_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1106413.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(37040..37936)
                     /locus_tag="SCL80_000522"
     CDS             complement(37040..37936)
                     /locus_tag="SCL80_000522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106414.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(38111..39400)
                     /locus_tag="SCL80_000523"
     CDS             complement(38111..39400)
                     /locus_tag="SCL80_000523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1106415.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(39372..40889)
                     /locus_tag="SCL80_000524"
     CDS             complement(39372..40889)
                     /locus_tag="SCL80_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEG1106416.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            41087..41977
                     /locus_tag="SCL80_000525"
     CDS             41087..41977
                     /locus_tag="SCL80_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEG1106417.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRGKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCL80_000526"
     CDS             complement(42050..42874)
                     /gene="nadE"
                     /locus_tag="SCL80_000526"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEG1106418.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(42886..44358)
                     /locus_tag="SCL80_000527"
     CDS             complement(42886..44358)
                     /locus_tag="SCL80_000527"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEG1106419.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(44995..45357)
                     /locus_tag="SCL80_000528"
     CDS             complement(44995..45357)
                     /locus_tag="SCL80_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1106420.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            45580..46332
                     /locus_tag="SCL80_000529"
     CDS             45580..46332
                     /locus_tag="SCL80_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1106421.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWLSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(46472..47395)
                     /locus_tag="SCL80_000530"
     CDS             complement(46472..47395)
                     /locus_tag="SCL80_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1106422.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYQKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(47410..47682)
                     /locus_tag="SCL80_000531"
     CDS             complement(47410..47682)
                     /locus_tag="SCL80_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106423.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        13979 a         9954 c         8268 g        15751 t
ORIGIN      
        1 aaaaatttcg tcacattccc ctttttcgta tcctatttta agcgacttgc ttggcataac
       61 ggttcacgtt ttgcttgata ttcccgattg ttttcggatc attgattcct gtgacttctt
      121 gaatcaattg ttccaagttt tcatggatct tcatttcatg caattgacgg ctttgttcat
      181 cttcaggatc atagtaatta aaaataatcc ccattgttgc cgtcaaatgt gggcaagtca
      241 agttatgttc ttgtagttct ctcactggac gtgtaaatct ttcttgataa cctaacttac
      301 gtaatggtgt ccgagcgaca cgtgtgatcg catcagagat atttttgttt tggaagcgat
      361 gaatgatttt ttcaatatac gcatgatgct gctctgcatc aaacccccat ttggcaatca
      421 gcaaacttcc tgtttcctgc aaaactgatt ttaattgtgc cacaactaac gcatcttgca
      481 ttgcttcatc aatggtctgg taacctaaca aggcacctgt ataagcgaca gtcgcatgac
      541 cagtattgac actaaatagt ttgcgttcga tatatggttc tagatcatct acatataaaa
      601 ccccttttaa ttggattttt ttattttttc tttggctatc gtcaatgacc cattctttga
      661 aaggttccac ttggacgaat aaaggatctt catggtgttg taatgggacg attcgatcga
      721 ctgctgcatt tggaaagccg atatatttgt cagtaaatga tgtttccgga agatatttat
      781 acacttcttt ttctagaaat gctgaaccgc caatcatatt ttcacaggca atgatatcta
      841 aagatgttgt gcttccttcc ttttctcttt cttggatacc ttttgcaatc aactcagcaa
      901 taaaaggtaa gatattcggg ccaatggcgg tagtaactaa atctgcttca gcgatttctt
      961 ggattacttt ttctggattt gtttgattgt tgattccttt gacatttttt acagtgattt
     1021 tttcttttga ttcatctgct aattcgattt cgtattcttt tctttcattt aaagcatcaa
     1081 tgatcgtgtg attcacatct acgaaagtaa tctcaaatcc attagctgcc aaaacctctc
     1141 caataaatcc tcgaccgata tttcctgcac caaaatgtac tgctctcatt tttctctctc
     1201 cttaagcaat tgctagattt tccgttactt cttctttgct gagcgcgtct gctagctgga
     1261 caacattgtc catatcactg caataaagtg cgatttgctg tacaagttgc aaatgctcat
     1321 ctccgactcc tgcaataccg aacaaaacag tcgcaatctt ttcttcttct tcggtaccaa
     1381 aatctacacc atctggtact tgaacgacac agatgccgga ttttttcaca tatttttttg
     1441 cttcatctgt tccatgagga atcgcgataa aattccccat ataaacagat aacattttgt
     1501 ctctatctac catggcatcg atatattcct catcaacgca ccctgcttct acaagcatct
     1561 gaccgcaata gcggatcgct tcttctttgg ttttaaaaga tcgattgagc tcgatcatgt
     1621 cgaatggcaa actttgcatg ctgttttcct cccttaagct gtctgtttca aatattgtgc
     1681 aatcgattcg tatttttgcg gattgaccat gctgttgact gataaatgaa tggattgagg
     1741 tgcagccatc tgtacttttt ccgctaaact ttctttggct acgatcagtg aatcaggttc
     1801 atcttttaaa tcttggatat tgacttttct taacgggata gttaaatggt tcttgtctaa
     1861 tatatctttg agcacggtca tccccattgt tgctgatccg gaacgatcat cgtggacaag
     1921 tagaatctct ttgatttgtt tgatgtttgc tgaaagaaac tcttctgctg gctcattctc
     1981 aatttcagaa actgatgttt tctgcttgct gcttaattct tgaatgattt catcatatcg
     2041 aggtgaattc aaaaagtttt ctactgagac aaaacgcgcg ctcggtgctt tttgttttgc
     2101 tctttcttgt agttcgacct gagtaacgat caatgtattc gagtcatctg tcagttggct
     2161 gattgcttga ttggtcaccg tttgaggcaa gtctgcttcc tgtacttttt ttctcaagat
     2221 cgaagcgccc atcgcacttg atcccattcc tgcatcacaa gcaaaaatga ttttttgtac
     2281 gccagagaat gtagctcctt gaggaatacc ttgggcacct ttggaagctt gcttagctgc
     2341 ctttgtctct tgaacagatt tttcgaagtc ttcatttgta tctttcgcat ccgctttcaa
     2401 aattactgca gaaataccaa acgaaacagc tgcggcaaca atcacgccca aaataacagg
     2461 aaggtaagaa ttgaacggtg tcatcgctag aatcgcaatg atcgaccctg gagaagctgg
     2521 cgctcttagt ccagcatcca tcaattggaa aatgaaactt cctgacattc ctccgcctat
     2581 aactgataag aataataacg gtttcatcat aacgtaagga aaatagattt catggatccc
     2641 gcctaaaaag tgaatgatca tggctcctgg tgctgaagat tttgctgctc ctttgccaaa
     2701 caaagtaaaa gcaagtaata cgccaagtcc tggaccagga tttgcttcaa gcaagaatag
     2761 aatagatttt ccagcctctg ctacttgttc agttcccaat ggtgtcaaaa tcccatgatt
     2821 gattgcatta ttcaaaaata agatttttgc tggttcaata aaaatacttg tcaatggaat
     2881 caaatgggca gataaaatga tctctacacc ttttgccatt gcttgagtca atgcatcgac
     2941 aactggacca atcgcagaaa aacctaataa cgctaatcca aaaccgataa gacctgctga
     3001 aaaattattg actagcattt caaaaccgga tttgatccgt ttttggaata gctgatcaaa
     3061 tttcttgatg gcatatccac caagaggtcc catgatcatc gcgcctagca tcattggaat
     3121 atctgttccg acaatcacac ccatcgttgc aattgcccca acaactgatc cgcggtcacc
     3181 agcaattact tttccgccag tataaccgat caataaagga atcagataag ttagcattgg
     3241 gcttaccatg gtagccaaag cctcatttgg taaataacca tctgggataa atagagccgt
     3301 taatacaccc caggcaatca gtgctccaat atttggcatg accatgcttg ataaagtact
     3361 gcctaatttt tgtaccctcg cttttagcga aatgttgttt ttgggaatcg tccgttccat
     3421 tttgtctctc cctctctcct tttgatactc taattgtatc cgcttactaa aaagagaaat
     3481 agtcgtactt tggcacgaaa ctttgtgcca aaggtttgag tatgtcagca aaataaaaag
     3541 atttcggtat cagaaaactt caattttaaa taaaactgtt attttaattt ttttataata
     3601 tgcaaacaat aacttttgtt agcatttaat ttagatgttt tccattctat tatgttatta
     3661 aattttatat tggaggccta tctatgacat tactgatcaa aaacatgaac atgaaacgtg
     3721 gtaaaaaacg tgtgattcga caattgaatc ttttagtaga atcaggagaa gtagctgctt
     3781 tgattgcccc gaacggattt ggcaaaacaa ctttgctgaa tggcattgcc caactgctgc
     3841 caacaactta tgatcaattg tcccttcaca acatctctcc tactgcttat acagattatc
     3901 gccggtcttt cttttttcta gaatcaacga atcagttatc cgactatcta tcggctgttg
     3961 attatttgac tttgatcaaa tactattggc agagcaaagt agaatgccaa actgttttcc
     4021 aacgattgaa gatgactgag taccttactt tgccgattgg aaaaatgtct ctaggaatga
     4081 aacaacatgt cttgcttgct gcttacttga tcagtgattc atctgtcatg ttattcgatg
     4141 aaccgttgaa cggattagac ccaggaagta ttgatatctt gaatagcctc atcagacaat
     4201 ggcagaaaga ggggaagacg attcttcttt cttcccacgc tatcgcgaat atccaatcga
     4261 tttgttctaa agcatgtttt ctaaaaaatg gagaaatcgt cacagaatcc tcaaatatgg
     4321 aagagatttt agctatttat cgtaaattat atgaggtgta actatgtctt tacttacatt
     4381 tgaacgcaat ctcttactaa aaaacaaagt aaacttttta tttcctctat tgttggtcgt
     4441 gctctttgca ttcccacttt tttttgatca taaacttgct tatacagaat tcgatgaact
     4501 gaatcataac tatgaagaga tgcaacgttt gattgaaacg ttgaaagaag atgagaatga
     4561 gaaagaattt gtggaaagtt tagagaaaag caacaaactg atcgaagcaa tcttacatgc
     4621 aaaaaacaca gggaatgtcc agcaaatggt cgaagcaact tatcattatg aaaaagatat
     4681 actggatcga ctgatttctg gtcaaagaca aggaattcca atcattgaac aacaaaaacg
     4741 tgtcgagttg ctccgttata tgaaagaaca tcagatccaa agatattcta tttttgactt
     4801 gccagcgcat ctccctttag caaattacta cgaaaatatt ttttcaggaa tgatttctag
     4861 ttttttgatt ttatgtataa ccgccttgtt tctttcttcc atcatctctt atgaaaaaag
     4921 aaagcaggta atctctctag tcaatctatt accggatagt atggtaaaga aacatagcat
     4981 ccgttttacc atttattatg gtgcagctat gctaagtctc gtgatgccat ttttgatcgt
     5041 ctctatacta gtaataatca aaaatggttt gggagacttc cgttatcctg taggaacgat
     5101 cattggtcag gagatacgaa tccttccgat gtatgagtat cttttccaga gctttctgtt
     5161 cctgttgttg tgggtgctct ttttaagtac aatcagtttt cttctatctg ctttatttga
     5221 acattctcta gtcaatcttt taggtacact tctttgtctt ttcttagcag agtatcgctt
     5281 gttttcttcg attggatgga tagaatcaat cagtcattac ttgccaactt cttatgttga
     5341 ttttcaaaat gtgatcatag gaggagatat tttctcgcca ctagcttcag aacaggttac
     5401 tttcatgaat ggtatcttga cacttggtat atggagtatc gttttattat tcataggtat
     5461 gggcactatc tatataaaaa agagctatta aaacacaaaa ataatagggg cggtgacaaa
     5521 aaccttgtca ccgtccccta tttcaaataa acggttttct agaataaata gttttcaaaa
     5581 gcttgcatcc gcagtaataa gaacctccca acaaaaatag ctcctcttat ttttatcttg
     5641 gttgaagtta ttacttgatg caaggcagct ttttttactg tgaacattgg ttatatatgt
     5701 ccatgttcaa aaatcatttg gacttcttca atggttttcg catcactgag cgccattact
     5761 tggtcgatgt ctgaacagaa aaaggctaaa tcttggagca ccgctaactg atgttctttt
     5821 tttaaagcca ctacaaataa gagtgtcgcg atttttggtt cctcttttgt tccaaagtcc
     5881 acaccatcag gcacttggac taaatagatt ccttcttcta ctacttcttc atctttgtca
     5941 gtcccatgtg gcaaggctac gaaattcccg atatagacac tcgctttttc ttgtctttcg
     6001 atcattgatt ggatatatgc cggtttgatt cccccttgtt gggtcatcca ctggccgatc
     6061 tcggtcaacg cttctttcca attttgatag gattgatcga gcaatatttt ttctgctggt
     6121 agctgtatca tcttttcacc ttcatttctt tatttcttca atcaggattt tcgctaacaa
     6181 ctgataaacg atttctttat ttcctgagcg gaagatctcg gtgttcaagt cattcatgat
     6241 gattgctcca ctgatctttc ctaaaagaat cccgtctttt tcttggattg gcgaaggcgc
     6301 cagcataact aacatacggg acaaatcgac tggctgtcta tccatccctt caatggtcaa
     6361 cggatgtttc aaattgaaaa tacaaaagat cggtttagaa atcaatgagc tggatgtatg
     6421 gaataacccc atatttgtat gaggaatacc gataggtgct tgttcatatc gcttaagcag
     6481 ccggcgatga accacctctt catccgttac tagtccttca ggaaaataag gaaataagct
     6541 gcgaatcgtt tcggaaatcg tctcaggatt atccagttct tggataaaaa aacgaccgag
     6601 taattgattg atttgatcca tgaaatgcat tgtttcctca taagtatctt ctatcatcca
     6661 ctcgttattg ctgttatacg taattttttg cctttttttc ttctcaatat gcgaaaaagc
     6721 ttcttttagc tgcttgactt catcttctag cagtagcggg gagactaaaa tatattttcc
     6781 accgtatcct ggtaaaatag atgtagaaat aacaacatca tattcttctt gtaaatcgat
     6841 tttttttaaa tcagcaatcc gatagaaatg aatttgttgg atgaagggaa aatacctttt
     6901 caatcgtagt tccaacatgc tagtagaagc taatccactt ggcgaaaatc ctgcaatact
     6961 gatattgctt tctttcggac ttcgttccaa tgaattcgca aaatgaagga ccatataagc
     7021 gatctcttct tcggataatt ttttttcttg aaaaatccgc tctactgctt tacggattgc
     7081 attcgcaact tcctcgtatt gaaccatgat tttttctaaa atcggattgg tcagtgtctc
     7141 ttcttgtaaa acggctcttg aaaacacacc agataaatga gtcaacagca ttttgtacaa
     7201 agtttcatct tcataaaaat ggacattcgt ctcttcactt acttcttgaa tcagttgttt
     7261 tactaaataa gcaagctgtg tatcgaacgt ctcttcaaag aaattctgat caaatgagtt
     7321 cgtgaagtca tttaataatc ctgcaaaaaa gacgatctca tttactggat aaagttgttt
     7381 tgtctcagaa gcgatccgtg aaaacaatcg tttagctgtc tgtagtaatt ctttgtttgc
     7441 cagactatta tatgtctcat ttgtcagtaa atgattttta gtcacacgct ccatcgtcaa
     7501 tgtcagcatc aaaaccaaat gttcaagctt tctatcactt agattggtct ttttttggat
     7561 caattcttcc ccgattactt tgcgtgcaag agatagatgt tgcggatcaa cgaatcgaaa
     7621 aaaataattc agtgatgcat cgaaagaaat actatgaaaa agttcatatt cattgatttc
     7681 taattctaat acattcgctg tcagaagccg gcggtatttt tccggtcctt cgatctcgta
     7741 tccctggttc cgaatgattt ccaaaggcag ttggcgaata ctttcttcca actgtttgat
     7801 atctgcataa aaagtggtat tactgatgcg atatgattcc atgaaatgag caagactcaa
     7861 cggctctgtc gtcagtaaca actcggctaa aatcgcgtgt tgtcgtttag cagtggataa
     7921 gtcattactt ttcccatctg tattttcttt taattgctct aatacttctt ctgatgcctc
     7981 aatgacaaat gagcctctag ctgctttttt caaagaagca tttaccgatt tcaatgagac
     8041 ttcaagatta tctaattcgc ggtaaatagt ccgtttactg accttcaata aattcatcat
     8101 tttttggata gatacaggtg acgggctact gagaaattct gctaataatt gtttttctct
     8161 gggcgataaa tacatcttat atttctccct tcacgagttt ttcgtattca ttcgtagcta
     8221 cccaatgttc aactagaact agccgaggga tcttttcttc tagctgttca gttacagcta
     8281 gttctctttg tgcgatataa atcgtttctt cttggatttt gattttttct attgattctt
     8341 ttgagataac aaactgtttt ttcgcttttt ttgctaattg acttaaaatc gtcatgccca
     8401 tagtttgtga gccgcgtctg ttgtttttat acaaaaatac aatattagca ggcgactttt
     8461 tttgttctgt gatatctgct atctcttttt tttcttcccg ctttggaaat tggatcgctt
     8521 cttttattcg tgacagcaag atatctgttt ccaaaaagtg atccagtaca tagatttgac
     8581 tttgaggtac tttttctctt gcaagacccg ctagttcatg ttgcacgata accagctgat
     8641 ttacttggtt attcaattca tagatagagc tgtatccgac agaaagatcg attttttctg
     8701 ctgctaattt ctttttcagc agggcggctc ccatagcact tgatcccata cctgagtcgc
     8761 aagcaaaaat gacggaatga atgatagatg gttccaagat ttctcccctt tctttgaccg
     8821 ttttgttctc tttttcttta tttggtaatt tcaatagtat cgctgcacaa atgaaggtca
     8881 caaatgtact gattgctatc cctatactta ccccagctag ttgtggataa ggcgcattgg
     8941 caaaaatcac gagtagtgac ccaggcgata caggtgcagc taatcccgta tctaaccatt
     9001 gaaatatcaa cgtgccgctt gccccaccag ccattacagc taaaaataga tacggtcgca
     9061 gcaagacaaa tggaaaatag atttcgtgaa tcccgccaat cgcatggatc atcaatgctc
     9121 cactcgcatt tactttttcc tgtttttctg caaaacaaag gatagaaagt aagatcccta
     9181 aaccaggtcc aggatttgcc tccatcaaaa ataaggaaga gtacccgact gtttgtgaga
     9241 attcgattcc taaaggtgtc aatacaccat gattgattgc attattgaaa aatagtactt
     9301 ttagaggttc taagatcaga ctgacaagtg gtaacagatt cattgtgact agccaagaca
     9361 caacggaggc gagcaacaag tgaacactat ttaaaactgg ttcaataaaa acgattccaa
     9421 ttatacaaat aacgctacca gtcagtccta ctagtaggtt gctgacaagc atttcatatc
     9481 cagccttgaa tttcacgtgg aatctagaaa caaattgttc atatacgatg ccggcaagcg
     9541 gcccgataat catcgctcct acgatttgtg gagcattgga acgagcgatc aaccctaaag
     9601 aagcaattgc accggcgact gccccttttt gtccccctac cgatttcccg cctgaataac
     9661 tgatcaagat gggtaaaagc agctggatca tccaatggca tacctcttct aatgtggtgt
     9721 gcaaatcgcc agacacacct tttgttgcta atgacaagat tccccaagcg ataaacagac
     9781 ttagatttgg catgatcatt tcatttaagt gatgtccagc ttttctcagt tgtttcatag
     9841 acacgcgact gctgttgttt gctttccatt tgtattccat ctttttcacc atacttctat
     9901 ttattgatgg acggatttta tgtagaaaaa gcagcaaacg aaaaaccgtc tgctgctttc
     9961 agctatctgg gaactatttg cctggttcag tcgcaatgat tgctagttca ccttggaccg
    10021 cccactcaga aacgccattt ggaataatac agcttgttcc tttttctaat ggatatgttt
    10081 ttccgtcaac gacaaggtct cctgctcctt cgattaccgt catcaatgta taaggggctt
    10141 gttttttgaa agatgtgatt cctttaatat cccactcata tacattgaaa aactcagttt
    10201 ctaaataagt gacgatcgat gaattccctt ttcggacttc tttgatttgt aattctggtg
    10261 ttttcgcagg gacagttgtt acatcgatcg attgttggat atgcagctct cttgtttgtc
    10321 cttgcgcatc ttttctgtcg tagtcataca cgcggtaagt cgtgtcagaa ctttgctgtg
    10381 tttctaaaat catgattcct ttaccaatag catggatcgt tccgcttggt acataaaaga
    10441 aatcaccttt tttaactggt actttcttca aaaggtcatc ccagcgccca tctttgatca
    10501 tctcagctag ttcttctctt gtctttgcat gatgaccata gatgatttct gcaccaggtt
    10561 cagcatcgat gatataccag cattcagttt ttcctaattc accttcatgt ttcatcccat
    10621 aggcatcatc tggatgtact tgtacagata gatcatcttc tgcgtctaag atcttgatca
    10681 ataatggaaa cactggttct gaaggatggc caaacaattc cttgtgttct gaccatagtt
    10741 catctaatct tttccctttg aattcgccat tttctactac actgacacca tgaggatgcg
    10801 cactgatcgc ccaatcttct ccgattttat cgttagggat atcaaaacca aagactgaac
    10861 gtaaacgact tcctccccaa attttttctt gaaaaactgg ttttaaaaac ataggttcct
    10921 gcataattca aactcctctc atttttcata gctattatag cattatttcg gtaataccgc
    10981 tttctctttc aaaaagtttt caacaatttc atctcgacat atgagatact ccgcacgatg
    11041 atcgacagga tgtacgcgat ttgacaaaaa tatcaacgct tcttgtgttt ttatatcaat
    11101 caagaaaaac gtacctgtat aaccagtatg aaaaagaatc gcatgaccgt cttctacatc
    11161 cttttttaag tcccatccta aagaacgata gccatttttt ttcggtgttt gatcttgcag
    11221 taaagaatca atcgtctctt cttctaaaaa acgaccatta cggatttttc ccttgttcaa
    11281 atacatttgg acaaaagtaa tgctgtcttt caaatcaata aagagacccg cgttacctgc
    11341 atgttcagcc aaaactcttg ctttcggatc atgagtttgt ccacgcaaaa cctcacccga
    11401 aggcagttgt tctgtgggaa cgattttttt ggaaggaaaa ggaagaaata agctgttcat
    11461 cattcctaat ggttccaata cttgttcttg aaagatttcc actgcagttt cttggtaaat
    11521 ttcctctagc atcaacccaa gaagaatcgt accggcatct gtatatttta cttcttttcc
    11581 tagttgctca cctggttcta gcttcaaata agcggcgatc aattcttcct gagatagctt
    11641 atctctgttt ggtatccatg ttttgatatc agaagtatgt gtcagtaaat gacgcaaagt
    11701 gattgtctga ttttcaaatt caggaagata tttatgaagc ggttgatcga gttctatctt
    11761 tccatcttct aatagacgta aaactaatga agtggttgca acgactttcg tcagtgaggc
    11821 tgcatcaaac aacagatctt ctgtcatcag ctgcttttca ggaacgactt gggcaaagcc
    11881 ggctgtaaat gtttctactt gattttcttt tatgaaggcg actgctacac ctggataaaa
    11941 tccttcttcc atctttttca aaataagctg tttcgtgtgt ggatacatct ttttctcctg
    12001 cttttctatc agattcttac agtccaagct taaagcttaa tctatgagga atcaaccttt
    12061 tttacacaaa aaaactgcgc gaaaaaccgc gcagtttccc ccggtatact attttctctt
    12121 tctatctcta gaataagcga tagtatgacc gttcataaaa tgacgagaca tgcgacaatc
    12181 gttaagaaag aaaaagttaa gcgtcgcatc gaatgtcagt tttattcttt tcttttggct
    12241 cactgacgaa ccaccattcg attcctatag catcatagat tgtgagtact gtgtgctttt
    12301 tgtctaagaa gaaatcaact gatagttgct gcaagtgagc aatcagtcct tccatgcttt
    12361 cagtatcgat catgaatttc agaaaatcaa gtccaatcac ttcatctgta ggcaaatcaa
    12421 ttgcgcctcc gctagcttct gtcaaaccaa catggaaatc tccttcattc aatacatgga
    12481 tgccctcttc ttcattatgt accttcaaac caagtacatg attaagaaat tggcgttctt
    12541 ttgttaagtt agtaacattt aaatggattt tttcaaaata agaacctgtt gatagtttac
    12601 tgaatttttc ttcagatttt cttaataatg cttcttgatc cattgtttct ggaatttctc
    12661 tttggtcgtt cttattctct tcatggtaaa tttccaattc attctcttct gggtctacga
    12721 gcaaaatccc catacgtcca tgatcttcta aagcttcttt gattggataa ttcattttac
    12781 ggatacgaca aaggatatct gccatttctt ctgtgctagg gataatcaat gaaaaacggt
    12841 tcagcttttt gatcaccccc atgtgatcat tcgctctagg actttcttcc agcagcaaca
    12901 tttcacttcc agcttcttct gttcctaaaa tggctaattc gttttcttct cttttcaaat
    12961 caaagccaac aacgttcttg taaaaatcaa tcattttatc tcggtctttc acccgaatgg
    13021 ctacactttt taaataggtg gcagccccca attgaaaatc tttcatattt cttgctcccc
    13081 tctacgtcaa gctttatttt aacgatggat tcttcttctt tcaagcaata actttgattt
    13141 tgttaaaaaa aatgtgagaa aattcgcatt ttgcttgttt tttgtaaatt aatgagtaaa
    13201 aactcttata aaaagaggtt gtgaaaaagc tgttctgcat caagtaataa gaacagccaa
    13261 acaaaaatag ctcctcctat ttttcgtttg gttgggctta ttaccgcaga tgcaagcttt
    13321 tgaacaccgt ttaaggaaga ggttgtaaaa tcagcggttt gacctgagta ttagagatac
    13381 aaaacggaaa aatagcttct catattttgc cgttttttga gctaataccg aaggacagct
    13441 gattgaacac cgtttattcg gaaaaaggga ctgtgacacg acttttgtca cagcccctaa
    13501 aaaaaataat aaatttgtac tattctatga agcttcttct tcagaaaact gactgttata
    13561 aagatctgca tagaatccgt ctttttccat caatgtatcg tggtttcctg tttctacgat
    13621 cgaaccatga ttcatcacaa taatgttgtc tgcatcgcgt attgtagaca agcggtgagc
    13681 aacaacgaaa cttgtccggt tttccaatag gcggttcatc gctttttgaa tcagtatttc
    13741 tgttcgtgta tctacactag atgttgcttc atcaaggatc aggacatctg gatttgctaa
    13801 gaatgcccgc gcaattgtga ttagctgacg ctgcccttgc gaaatattgc ttgcatcctc
    13861 gtttaataca gtgttatatc cttctggcaa tttacgaaca aaatcgtcta cgtgagcagc
    13921 tttagcagcc tcaatgattt gttcgtctgt tgcatcgtcg ttcccatatt tgatattgtc
    13981 atagatactt cctgtaaaca gccaagtatc ttgcagtacc attgaaaact gtgcgcgcaa
    14041 ttcatcacgt gaaagatcac gggtatctac accatcgtaa cgaatactac ctccagaaac
    14101 atcgtagaac cgttcaagaa ggttgatcag tgtcgtcttc cctgcaccag tcggacctac
    14161 aatagcgacc atctcaccag gtttgacatc caagttaaaa tcagtcatta gcaagttatc
    14221 tggtgtgtag ccgaattgaa cattttcaaa acgaactttg tatggtgtat ctttttcttc
    14281 tggaaggcca gatttttcat tggacatctc ttcttcatcc aacacttcaa atacccgttc
    14341 ggctgaagca actgttgatt ggatagtgtt catcaaactt gcaagttgag tgattggttg
    14401 agagaactgt gttgtatatt gcaagaacgc ttgaacatcc cctaatgtca tgttcccatt
    14461 tgcaactttt actccgccta gtacagcgac gaacacatag cctagatttt taacgaaatt
    14521 catcaacggc atgatgattg ccgaaataaa ttgagctttc caaccagcgg catacagatt
    14581 ctgattttct ttttcaaata cttcctggtc tttttcagta tgattgaatg ttttaacaat
    14641 ggtatgacca ccatatgttt cttctacttg gttattcaat aaacctaaac ttttttgctg
    14701 atctgcgaaa aacacttgtg atttcggcgc tacgatcatc acgatgatca agcttaaagg
    14761 gatcatcaat aacgcgacta acgtcaattg ccagctgatt gaaagcatca tcacgatgac
    14821 cccaatcaat gtgactgcac tagttacaaa ttgagtcaaa ttttgctgca gcgtgcttgc
    14881 gatgttatcc atatcattga ttgctcgaga catgatatcc ccatttgtat gtgtatcata
    14941 ataggaaatc ggtacacgat tcattttggc ttccaaatca tgacgtaatt cataaactgt
    15001 tcgttgggaa acacgtgtca taataaattg ctgtaaaaag ttgaatacag cagaaatcaa
    15061 atacatcacg attactgtcg caatgatctg cgcgatttta tcaaagtcta tcggaaattt
    15121 tgttacttgt tggccttgtt gcatctgttg agcgccagcc attacacctt tgaatatttc
    15181 tgtggttgct ttccccaaga cctttggtgt ttggatttgg aagatcacgg ctgctattgc
    15241 tagtacaaat acagcgatga tcgcaaccat acgtttagac atatagccaa acaatcggcg
    15301 taccgttccc cagaaatttt taggcttttc accttttaca ccgagatttc tgccaggtcc
    15361 accatgtcct ggaccgcctg ctggtccatg agagggacgt gtgtttgttt tactcatgct
    15421 aagtcctcct ctcttagttg tgaatgaacg atttcttgat aagtttcgtt tgttgctaat
    15481 agttcttcat gtgttccttt gccaaccagt tttccttcat ctagcacaag gatcatatct
    15541 gcatccatta ctgtacttac tcgttgagca actaaaacgg tgatactttc tttcatattc
    15601 gttttcaatg cttggcgaag attggcatcc gttttgaaat ccaacgctga gaaggaatca
    15661 tcaaacacat agatatctgc tgttttgact aatgcccgtg caattgccag acgctgtctt
    15721 tgtccaccag agaagtttcc tcctccttgt tccacatggc tatctaagcc atcttccatc
    15781 tctgaaacaa aatctttagc ttgtgcgatt tctaatgctt tccaaatcat ttcatctgtt
    15841 gcatccggct taccatactg catattgtct cggatcgttc cagagaacag aactgctttt
    15901 tgaggaacaa agcccattaa ttctcgcaaa ttatattgag tcatgtcact aatgtccgtt
    15961 ccatttattt ggatcgaacc agattcgatg tcatataatc gagggatcaa attgactaag
    16021 gtcgttttac ctgaaccagt cccccctatg attgcgacga tctcgcccga cttggcttgg
    16081 aaatcaatat cttccaatgc aagtttttct gctccatgat atcgataatt gacatgattg
    16141 aaggccaatg ttgcgttttt tccagcaaaa gaaactgttt taggattttc agggtcatgg
    16201 ataccgattt tttcatccaa cacttcatta atacggtcag ctgaagcttg tgcacgaggc
    16261 accatgataa aaatagcgga aagcatcata aagctgatca agatctgcat cgcataagtc
    16321 ataaatgcaa tcagattacc gacttccaaa gtcatatctg ctatgtagtg tccaccaaac
    16381 caagtgattg ctacgtttgt accgctcatg atcaatgtca tcattggcat catcaatgcg
    16441 acgatcgtat taactttgat tgccgtattt gtataatctt tattcgctaa atcaaaacga
    16501 ttttcttcaa agcgtgtctt atcgaatgct cgaatcactc tgacccctgt caatccttct
    16561 cgaaatacca ggtttaaacg gtcagttttc ttttgcatac ttttgaataa tggaacagca
    16621 aagtacatga ttccgccgat caaaataatc attactggga tgacaactaa gaagattttt
    16681 gtcaactgat gatctttttg ataagcaagt atgctcgcgc caatcaacgt gattggtgca
    16741 ttgatcatca tacgtaaaaa catttgtgta accatttgga tttgagtcac atcattggtc
    16801 gttcttgtga tcaaagaggc cgttccatat ttatcaaacg catcttttgt cagactctct
    16861 gattttttat agacatccgt acgcaattgc ttacccagtt tctgtgattc acgagtagca
    16921 aaaaatgtat ttgcgatagc agctaagata ctgatcaaag aaaagccgat catcacaaaa
    16981 cctgtatgcc aaatataatc gacatctcct tgcgctaccc ctttatcaat aatattggag
    17041 gtaagtgtcg gcaaatataa atctgccagt acttggatca ccatgaaaac tgcagctgca
    17101 attgctgaag tcaaggagat ccgttttacg atttttagca tctagttccc ttctttctat
    17161 ttatatactc cgtttttcag ccaagaaagt ttcaaattga attcactgat tgcttgctct
    17221 actgagtaat cttcagatgt tgaaaataag cgatacgaat gcgcaattgt tgtaaataca
    17281 gtattcatca ctaattggat cagtaattcc aattcatgat cattttttac tttcagcttc
    17341 gtccgatcga tcaattcaag caactctttt gcatcttgtt tgtgttcttt atgtggatcg
    17401 tgaggcgttt tttggcgact cgtatgagga gcgacttttc ttgaggcacg gtaatccatg
    17461 ttcataaaca agttacgata aaaagaagca ttctcgcctt ccaaaatctc ataaatcatt
    17521 ttagaaaaat aggattcgaa tcctttgaat agatcaccat cggattcttt cattagttgt
    17581 aacatatctt tagagctgtc acgtctcatt gtctcaaagt aataaaaata aagatcctct
    17641 ttatcttcaa aatactgata gaaactgcct ctagggatat ccgctaattt gacgatatta
    17701 gcaatcgaag cttcttttaa gggtactcta gaaaactcga ttctcgcagc ctcgattagt
    17761 cttttttgtt tttctttagg caaatggaaa aatgtctgtg tcggcatatg gatcctcctt
    17821 tctttcaaga tgacacattg tcatatgacg ctgtgtcatt ataacacctc ctacctgttt
    17881 tgcaagacat ttgcatagtt tttttcatta tcatccgata cattaaaaaa gtgataagag
    17941 gaatccttcc atttgaatta atgagagttg cccattaaaa tagccacata ctaaacaaag
    18001 gatgagataa atggcccaat caattagtgt ccaagacttc aataaattgc ctctcgatgc
    18061 tgacagcatc gttttagata caagaaatac cttagattac acaaaaaacc acttaagcca
    18121 ctcgctttct atccccgctg ctatcttgcc aagacaatta aaaaatctag atccaaagaa
    18181 actttattat attctaagct atacaggcag gcgatcagaa gcattagccg atcaacttac
    18241 tgctaatggt tttcgtgctg tttatatcat tggtgggatg gaagctcttc agtcgagtgt
    18301 tgcttgaata ttgcttgtta gattcaaaag aggctgtgac aaaaaatcgt gtcacagcct
    18361 cttttatacc ctgtcttttt ttagttattt attcttcaac cttatcaata tgacttgata
    18421 gccttttctt tttctcatag ttataacaat catagcaata aaaggagaag gtaagcatat
    18481 ggcatctgtt ttagtagtca aaggtcatcc cctaactgca gaagaatcgc gcacagttaa
    18541 ggcattgacg tcttttttaa caagttataa agaaaatcat ccagatgatg aagtgactgt
    18601 tttggaactt tatcgcgatg atattccgga aatcgatgaa gaattattat caggttggga
    18661 agcattacgt gctggtgcag aattcacttc cttatcagaa agtcaacaac aaaaaattgc
    18721 tcgtttcaat gaattgactg atcagttcct tgctgctgat aaaatcgtta tcgcaaacgc
    18781 cttatggaat ctaaatatcc caacaaaatt aaaagcttgg ttcgataccg tcaatgtagc
    18841 tggaaaaacg ttccgctata cagaaaacgg gccagaagga ttagtgacag gtaaaaaagc
    18901 actgcacatc caatctaatg gcggtgtata caacggacaa gactttgcct ctcaatatgt
    18961 aaaaggtatt ttaaactttg taggcatcga tcaagtagat cagctattca tcgaaggaat
    19021 cgattatgac cctgaccgtg cagatgagtt gatgcaaaat gcattagata aagctgctgc
    19081 tttaggtaaa tcattttaaa aattgagata aatgcataca aaagagacag aggaaaaaat
    19141 cttcaaagca gcatttgtac cttggatatt ttcccatctg tctctttttg ttattctttc
    19201 tgctttttca acggattttc ttcataaaga gaagagattg ttgtgtacca ttcaaaaaca
    19261 tgagtaatca ctactttagc tgctgcataa acaggaatcc ccaagatcac accgatgatt
    19321 ccgaacaatt tcccagaagt cagcagaact aataaaatcg ttactggatg gattgctaac
    19381 tgattcccta gtactaatgg agagatcaag cgtccttcta ttgtctgctc tactgcaaaa
    19441 acgatcagta ctttgatgat catgaatgga cctgctacaa tggctaaaaa gatagccgga
    19501 atcatcgcta aaaatgatcc taagtaaggg atcaggttca aaaaacccgc aataatcccc
    19561 aacgtgacag catactctaa accaatgacc gcaaaaccaa tcataaacat gattgccaca
    19621 gcaaaagcca cagttagttg tcctcttata taagaagaaa cttgactatt catttcactc
    19681 agcacattca acgtcggttt ttgcatgcgt gtcggtaaaa atttgacaaa ataaggtgct
    19741 aattgtttac cgtctttcaa tagataaaat aagataaacg gcatcgtcag cagagccact
    19801 aaaatagtcg ctaccgcgcc gacaaaactt ccaagactct gaactgtact cttagatata
    19861 tcttggatca tatcgcctgc cgaattcatg aacttatcac ctgctgtttc caactgttcg
    19921 cgaaattggt tgaacagcgg gtcccgcaaa atctcggtta atttattatc aatcgtatct
    19981 acatattgag ggaagttact gatgaagctc actgtttgtt cctgaatctt agggatgatc
    20041 actacgctcc cccatgcgat tagtgcaaca accaaaacaa acagcccaat gatactgtaa
    20101 agccttggta cttttcgctt ttcaaagtaa tcaactaccg gattcatcaa gtaatacaag
    20161 attcctgcta aaatgatagg cagacctacg attgctaaaa attgccagat tggtgcaaac
    20221 aaatatgaaa cttttgtaaa taaaaagacg atcagcaata ttaacaatac aacaagtaag
    20281 gctgtgacta cttgattatt taaaaaccag cgccaaaacc acgataccgg tctgttcttc
    20341 ttattttttc cttccataat tcctcctgtt agttataaaa aatttggctt cccaaaccaa
    20401 tagcaggcag tctatgcgga tgaagataga ttccatttgc aatttgtcct tcttctggac
    20461 aagcatcaaa aatcaatgat acgtgagcta ctgtctctaa attcccattg gccatcggac
    20521 cgacataatc gatcgtatat acctgatcat caaaagcaat tgttccgcct gctttcaaag
    20581 aaaaaggttc gtcttttgat acttcttgaa tcaccgaata ttttcgtaag gcagctgttg
    20641 ctgttttatc gaacaaaatc agcatgggtt cttccttgtt tattgcttct gtcccaattt
    20701 ctgtaatctt tgcctgaatc attgcactct ctcctttaat tgctttcaaa aacagtataa
    20761 cacattctca ttctttagcc gtgtggaata attatgtgat agaataaaaa cgaaaactat
    20821 ttcattacaa ttaggaggaa tcagctcata tgatcgaaaa gataatttta ggaacctata
    20881 cgcgtcgcga aagtaaagga atctatacga tcgaccttga tacagaaaaa gaagaattag
    20941 ccaacctttc attagctgtt gaagaaaata gccctaccta tgtagcaaaa agtaaagctg
    21001 gcaatcttta cacagttact agtgtggatg gattaggtgg tgccggtgcg tacgataagg
    21061 attttcattt tttgaatgcg gtaacagagt ctggcgctcc tttgtgttat gtagcggttg
    21121 atgaagcacg tcaattacta tacggggcga actatcataa aggtgaagta aatgtctacc
    21181 atatattagg agatggcgaa ctgaaagcag cagattcaat attccatcaa gaagcgacag
    21241 gtcctcataa aaaccaagat catgcgcatg tccactatac ggatctgact cctgaccaac
    21301 gattagtcgt atgtgatctt ggtacagatc gtgtgtatac ttatgatgtg tccgaaaacg
    21361 gcaaattgaa tcttgtaact accttcactg cagagccagg aacaggccct cgccatcttg
    21421 tctttcatcc aaatggacaa ttcgcttatt tatttggtga attagacagc acagtccgcg
    21481 tgctttctta taataaagaa acaggtgctt ttgaagaatt acagaaaata tcgaccttgc
    21541 cagaagaatt cgacggtgaa aatggagggg cagctatccg catttcaaat gatggcaagt
    21601 tcctttatgc ttctaaccga ggacataact cgattgctgt attcgcagtt tttgaagatg
    21661 gttcccacat tcaaaacaag caaatcatct caacagaagg ggattttccc cgcgatttcg
    21721 cactagatcc aagcaataat tttgtcgtgt gtgccaatca gaacaccgat aatctgacat
    21781 tatatcgccg tcatcctgaa acaggattac tggaattact gaaaaaagat gtctatgtac
    21841 cagaatgtgt ctgtgtttat tttgatcgtt agtaagaaaa ggagaatgaa atgaatcagc
    21901 aagacgtatt agacagacta cgggaagaac taaatattcc tttctttgat gggaaaatcg
    21961 aagataaaga gtatagcgaa gaggaatatc agaaactcaa agccgattta cagaactact
    22021 ttgaacaata tgtacagaat gtagaaaact aatcagcttt ccatactatc tgaaaagaac
    22081 atcaggacaa gacttttccg tctatcctga tgttcttttc tacaaataaa aaaccgccga
    22141 gatggttaga ctcgacggaa aaggagtatt tactcgattt tgagtaagta caaggttgtt
    22201 gttggttata ggtatgtcat tatcataaag gattttgatc actctatcat aaaggtaata
    22261 ttacatttat attagataac tctagttttt attgttatca aagatttctt ttattttttc
    22321 aacaacaaat tagtcatttt ttggatggca gctgtcaatt ggtctgtcgc ttggtcttgt
    22381 tttgcttcag atgtattgaa tagatcttca tctacccctg attcaagatc gatactttgg
    22441 atcatttctt cacagcctgc tacatatgaa agataagctt tttcaaaatt tttatgaatg
    22501 cccattgctt ttgctggtgg acgcaaagta ctgactttct taagcattgc ccgatatttt
    22561 tttgttcctt cttcaaataa caggatgatc tcttcaagag tcgatttgtc cagttcactt
    22621 gtttttcctt catcaattgc gtttctgatc acttcatatt tcggatgcat tccttctccg
    22681 attgcttctg tttccttgac gatttgatcg atggactgca cataatagcc aatattcggt
    22741 aacatgttcg ttttcctact ttctccattg ttgttattat ttattctact tgtttttatc
    22801 ctcaaatgac aagtacttga aaaaatttaa tggtttctat acagctcgcc attcttattc
    22861 gtgctcaaag aaaccatctg ctaaactata ttgtttccct gcgacaatgt catattggat
    22921 caattgatat tcgtcgtaaa tctcttgttg cgagcgattg aattgcgctt ctttatacca
    22981 ctgtccgttt tgatagtaca gttctcgttc aaacgccgga agttcctgtt ctaatgataa
    23041 aagcagctga taaaagccat ttgttgtctg attcgtctgt tccattagat tcgctgcaaa
    23101 ataaaacgga ctggtgattg catcgtgagt cgtttgcttt tctaattctc ctttactgtc
    23161 gaacataaga aattgtgttt catgcaatgt ttgtttgtca ttttttgctt ggatcgtatc
    23221 tgaatagatc cctggaagat gatcccccca aaaaacaact aaagtccttc tagaaagatt
    23281 cttcaattct tccgtgaact gcttcagtgc cgtgctgcta taggaaatat cttgcagata
    23341 attctctaag tcaagtgtcc cacttccatc tgaagttttt gcaggatagt ctagattatc
    23401 atactttcca tcgtagggca tatgtgtctg catcgtgact aaatgaatga actgaggtgt
    23461 tttttcttcc ttgagaagtg tcattacttc tttgtaagct gactcatcag aaatatatgg
    23521 attattctct atcgtatccg tataggtcat cgtacgctcg ctgatgaact ggtcaaatcc
    23581 taatgtctga taaacatcct ctcttttata catcgaagta ttgtatggat ggatcgctgt
    23641 cgtctggtat gattgtgcgt ttaacgctga aacgatagaa ggaagctgat ccatttttgg
    23701 tacaagcatc gtgtaagggg tggtgagttg cgcattaaat aacgccatag aaaaaccagt
    23761 caatgcttca aattcgatat ttgctgttcc accgccatag ttttgagaaa gcatattgcc
    23821 actatatgtt tgatcagcta cttcataata atctgccaat ggctcaccag atacttctac
    23881 gccgtttaat cgactaggat ctgagaaact ttcactcatc acgaacacga tgttcggcga
    23941 ctcttcctct acagttttat ttttttcatc ggctagcttc tggtattttt cagtgatttc
    24001 tttgatcttt tctttcgaat atccttcagg ttcatccatt ggttctacct tcagattgta
    24061 caaaaaccca ccgataaaac cagtgttata gtagttcatt ttctggctgt aaggtatcca
    24121 tagtgccgtt ttattatatg cttttcgcaa caaattatcg ggattgttga aattactgat
    24181 ataccctagc agtccaattg ccacaagcag tgtcgttaat cggatcaatt ggaatcgcct
    24241 tccttttttc aggcttcgaa acatgtacca gcaaaacaat cctatcacgg ttcctgcggc
    24301 agctaagatc actgtaaaca gcatagtgcc ggtcatatct ttcagcaatc cgatttcagt
    24361 gatcatcttc aaatcgtctg gataaatcgg ttcttgccga taactcattt tcatataatt
    24421 tgcaaaacct aatacaccga tggctacgac atacgtaagg gaacctaaag ggacagatcc
    24481 tgctagtgca atcagaaaaa taagaataat cagcaagacg agacaggcta agaagaattt
    24541 ttccgtatgc caagaaaaag caaatttcaa cgcaaggtct acggatagat cattttgaca
    24601 ccactgcaaa aataaattac taaaaacaac aagaaaaaag atactgatga ttgctgctgt
    24661 tccttttaaa atctttttct gtttggtcga aagtctgctt atatatcttt tcaaatgctt
    24721 gctccttcat ttctatcaat aaaagaatgt tgcttctctt attttagttg tacgaaattc
    24781 gaaagtaaag aacggaaaga agaattaatg aaatccaaat attctctttc tttatggcct
    24841 ttctggtttc tttaataagc ggacctttcg tactccagaa attttttcta gctgttgttt
    24901 cacttgattg agctgttcaa aatcagtact gtctatgtca atcaatatgt agacatgctc
    24961 attcttatgg ttacgcgata tatctgcgat cgctagctcg ttctcattca gcagagaaaa
    25021 tatctctgct aatatattgg ttttcttttg ataaaatata gtgaatcgaa acggcgcttg
    25081 gaaaaacatt cgtgcggatg gataattgac cgcttctcta accgtaccga acaaaagata
    25141 atcttttaag gcttcaactg ccaaacgtcc gctgtctgcc aaagcttcct gagtcgatcc
    25201 ccctagatga ggcaacataa gtatacggtc attttctaag aactcttctt ctggaaaatc
    25261 agtcaagtat ttagccagat gttgtttttc caatgcttgc ataacagcat atttatcaac
    25321 aatttcactt cttcctacat tgataagtac agcatctttt ttcatttttt ctatattcgc
    25381 ctgatcgatc aatccttttg tatcttctgt caacggcagc atgactacga tgtaatccga
    25441 ggtagaaaga actgtttcta aatctgcttg ccgaacataa tccaactggg catcgcggat
    25501 agaataaccc aagacatcca tccctaagct atagcaactt agcgctactt ttgtgcctat
    25561 cgctccaaga ccaagaagac cgatggtttt gccttgcaat tctcgtccca cataagcact
    25621 tcttttgttt tctgcctgtt ctaaaatatt aggacctgtt agtgtttgta ccatacgact
    25681 tgcttcgatt atcggacggg aagataagag caaacagcac aaaacaagct cttttactgc
    25741 attagcattt acacctggtg tattcatgac gatcgtcccg ttttcagaag ctttttcaac
    25801 gttgatcgtg ttcaccccaa caccagctct tgaaattgct agcaaatctg gtgtaagcca
    25861 ttcatctttc actttcgtac tacgaataac tattccttcg gctggcaaat catctattat
    25921 tttgaaatat tccccttgta acttttctat ctcttgttct ggaatagcat caatcaaatg
    25981 aacgtgatac ataaaacccc ctccttacta acaagtatct cgaaatttct gcaattagac
    26041 aactgtttac catttgactt cctagaagca aaaagagcag ttagagcaga gtaggctgag
    26101 tgataaaaat aaggctatga caagttactg ccataagcct tattttcgag agcaaaatat
    26161 tgagggtgag atatcttgat aaatggtgtt ccgttagctg acattgataa taagactaaa
    26221 tttcaaaaaa gttgaaaagc aaatttcgtc aatctcttct tatttcttaa aactaagtgg
    26281 ctttttcaca acctcttttt atctaataac cactgtttcc aactcaacta attttgccca
    26341 cgtttcaatt tgatctgttg ttagatttag cgagacaacg gtatgatgac cgccacctgc
    26401 ctgaatccat gaatggacac cttcatggaa attcggttta gttttccata acacacgtgc
    26461 aacaggtaaa ttaggtgctt cagtcgttgg ttcaaaggct tctacttcat tgatcagcag
    26521 tttataatgc gtaccgaaat ctgccatcga tacgactacc ccttctcctg ctttcccatc
    26581 aaatacaaga cgcgccggat cttctctatc tcccattgat aaaggagaaa caacgattct
    26641 tggtttcgtc gcagcaagcg tcggatctac ttccatcata tgggattcca agattgcttc
    26701 ttggccacta gctagctcgt aagtataatc ttccatgaat cctgtatctt tccctcgagc
    26761 catgattttc agcaaccgat caatcgctgc agttttccag tcgccttccc cagcaaaacc
    26821 gtatccttct gccatcaaac gttgtacagc tagtccaggt agttgtttca ttccatacag
    26881 gtcttcaaaa ttagtcgtaa aagcgttata gcctccttct tctaagaaac ggcgaatacc
    26941 gatttcctgc tgagcttgta cttttacatg ttcctcccat gtcttttctt cataatcacc
    27001 atagtcgaag gtatacaatt ccttgtactc ttcaaataat tgatcgactt cttcgtctga
    27061 tacgcgatcg attacttgta caagatctcc gataccaaaa taatcaacag tccaaccaaa
    27121 ttgaatctgt gcttccactt tgtctccttc tgtgacaccc acgtttctca tgttgtcacc
    27181 aaaacgagcc acttttattc caaaactttc attataggct actgcaacat ccatccaatc
    27241 agcaatctct ttttggatct caggtcgttt ccagtagcca acgacgattt tattttgttt
    27301 atttaatcga gcattgatga acccatattc gcggtcacca tgagcggatt gatttaaatt
    27361 catgaaatcc atatcgatcg ttttccaagg aatgctttcg ttatattgcg tagctaaatg
    27421 aagcaatggt ttttgtagaa gctttgtccc gcggatccac atttttgctg gtgagaaagt
    27481 atgcatccaa gtgatcacac ctgctacttc ttctcgataa ttgacctctt tcatgatttt
    27541 tgtgatcgta tcagcagtaa ctgctaattc ttgaaggcgg attggataag gcaattggcc
    27601 attttcattc agttcatcga ccatttcttg tgcatgtttc tttacttctc gcagtgcttc
    27661 ttccccatat aaatgctgtg atcctacaac aaaccaaaat tctttttctc caatattcaa
    27721 cataattagc cactcctttt atttgttttg tccgtaatac gcattttttc cgtgttttcg
    27781 taaataatgt ttatctaaaa tccgttgagg cagctcttct gaaaatgaat tgagctgtct
    27841 ggtaaataaa ttcatcttgc atacttcatc tagtacgact gcgttcatta ccgcactttg
    27901 cgcatctttc ccccaagtaa atggaccgtg tccgtgtaaa agtacccctg gaattgctaa
    27961 gggatcaatt ttgcgttctt tgaatgtttc tatgataact tttcctgttt catattcgta
    28021 cccgctgtcg atttcctgtt gtgtcaaaaa tcgagcacaa ggtaccgatc cataaaaagt
    28081 atctgcatgc gtcgtcccca tcgcaggaac atccaatcct gcttgtgccc agatcgtcgc
    28141 ccatgtcgag tgagtatgga cgattcctcc gatttgagga aatgaacgat ataaaacagc
    28201 atgagtaggc gtatccgaag agggattgag tttaccttca atgatatgat tgtccaagtc
    28261 cacaacgacc atgtcgtctg ctgtcatctc ttcataactg actccgctag gtttgataac
    28321 gaaatatcgt gtttcaggat caaaagcgct cacatttccc catgtatatt tgacgagtcc
    28381 gtgtttaggc aagtcaagat ttgcttggta tacttcttct ttcagttgtt ccagcatttt
    28441 ctatcactcc tttttcttgc aattgatgaa tcgctgtttt ttcaatgtcg atcccctcac
    28501 ggtaacgtct cataaatatc tgatagcctg ctatttcttc tgctgaaggt gtgatggtga
    28561 ttccttggtc ttgtccaaac acgtcttctg ccaagaagtt ttctaaagac ttcccatgtc
    28621 gatccatcat ataggctgct aataacgcga ttccccacgc tccaccttct ccggctgtgt
    28681 ccatcactgt aactggtgcc tccatagcag atgctagaat ccgctgtcca acttctggtg
    28741 ttttaaaaat ccccccatgc cccactaaac gatcaatttg gacattctca gatttcaata
    28801 tatccattcc gattctcatt gcaccaaaag cactggctaa atggatccgc atgaaattcg
    28861 ctaaatcaaa acggctacct ggtgtacgta caaataatgg ccttccttca tttactcctg
    28921 taatgttttc accggaaaaa taaccataag aaagtaatcc cccgcaatcc gtatcgcctt
    28981 ctagcgcttt gttgaataat accgtgaata atttatctct gcttatctcc atacccaaag
    29041 aattggtaaa ttcttcaaaa atactgaccc aagcattgat atccgaagag cagttattcg
    29101 tatgaaccat gcctactagt tctcctgaag gtgtagtgac catgtcgatt tccggatgga
    29161 cattttctag ttctttttct aatacgatca tagcgaaggc ggaagtacca gccgaaatgt
    29221 tccctgtccg tttggctaca ctgttcgtag cgaccatacc ggttcctgca tccccttctg
    29281 gcggacaaac cgggattcct ggtctcaaat ttcccgtagg gtcgatcaga taagcccctg
    29341 attcgcttaa cttgccagct tcttctcctg ctactagaac tttaggcaaa atgtgatcca
    29401 agtcaatcgc tacgtgcgct ttttcgatta actgaccaaa aatatttttc atttcttgat
    29461 ggtaggtttt cgttgaagga tcgataggaa acatgcctga tgcatctcct acgcctagta
    29521 ctttctcacc agtaagctgc caatggacat aacctgcaag cgttgtcaaa aaattaattt
    29581 ctttcacatg ttcttcttta ttcaaaatag attgatacaa atgagcgata ctccatctct
    29641 gcggaatatt gtattgaaat ttttccgtta atattcgttc tgcttcttca gtgattgtat
    29701 ttcgccaagt acgaaatggt actagtaact gttccttgct atcaaatgct agataaccgt
    29761 gcatcattgc actgaaccca atcgacccga ttgtcgtaag attctcgcca tagatttcga
    29821 atacttctgc tgccattttt cggtaactga cgcgcagtcc tttccaaata tcttctaatg
    29881 aataggtcca gataccattt tctagtttgt tttcccactc ataactacca gcggcaatag
    29941 gagtaaaaga tgaatcaatc aatacagctt tgatcctagt tgatccaaac tcaatcccta
    30001 aagatgtacg accatctatg acatctaaca tactttgcgc atttacttcc ttcataatga
    30061 tttaactccc tccttctcgt caaacctgat caaaccgtct ttttagtagc gcttacaatg
    30121 attattgtat acttttgtac gtacatgtca agcggttatt tacaagaata caataaaaaa
    30181 cacttgtttt gtacgtacat aagttataaa atgaaattgc ttacaaaatc tgatcaaccg
    30241 ccttctcgaa attctagaag gagagaatac ttatgaaaaa agaaaaaaaa gtttcctcat
    30301 cgtttattta tttttttggc gcatttggcg gcattctatt tggatatgat attggtgtta
    30361 tgaccggcgc cttacctttt ttacagcatg attggggttt ggcaggtaaa gcttctttga
    30421 taggatggat cacgtcttcg gtcatgttag gtgcgatcct tggcggctct ttatctggtc
    30481 ttctttctga taagcttgga cgacgaaaaa tgattcttct ttctgcactt atttttatgg
    30541 ctggttctgt cctttctgca tctgcccctc acaatggttc ttattttctg attgcagcaa
    30601 ggattcttct aggtttagct gttggcgctg catctgccct agttcctgct tatatgtctg
    30661 aaatggctcc tgctagacta aggggaagat tgtcagggat caatcaagtg atgattgctt
    30721 ctggcatgct cctttcctat gttgcagatt atttgctaaa agtgttgcct gaaacgatgg
    30781 cttggcgcgt gatgttaggg ctcgctgctg tccccgcgct gattttattt ttcggtgttt
    30841 tagctctacc agagtcaccg cgttttctta tgcaatctgg ccgcttggaa gaggcaaaaa
    30901 gagtattgaa ctatattcgg acaccaaaag aagctgaaca ggaattcgaa caaatccagc
    30961 tgaatgtcaa acaagaaaag acaaccggaa cttcttggca tactttgttt ttagaaaaat
    31021 atcgttcctt agtcattgcc ggtattggtg tagcagtatt ccagcaattt caaggagcta
    31081 atgctatttt ttactatatt ccgctgatcg tagaaaaagc gactgggcat gcagcgagcg
    31141 atgcattgat gtggccgatc atccaaggaa tcattttagt tgctggttct ttgctctttt
    31201 tggttattgc agacaagttc aatcggcgaa ctttattgaa aataggcggt agcgtgatgg
    31261 gactttcatt catcctacca gctgttttag gaactgtact ggatgctcat acaaattcgt
    31321 tgttgattct cttgttttta tgtatttatg tcgcatttta ttcttgcaca tgggctccac
    31381 tgacatgggt aatcataggc gaaatattcc cactagctgt ccgcgggcga gcatctggcc
    31441 tagcctcttc ttttaattgg attggttctt tcctagttgg cttgcttttt cctgtgatga
    31501 ctgcttctat gtctcaagaa atcgttttcg gcatttttgg gatcatctgc tttttagggg
    31561 ttttatttat tcaagagatc gtaccagaaa ctcgtgggaa atctttagag gaaatcgaac
    31621 aaagtgcatc taaaaaaact tatcctaaac gtatttctat ccatcattct taaagaggtt
    31681 gtgacagaag tggtcagctt caagaaataa gaaggaaccg cttagtttcc agtcacaagg
    31741 aacaatttca aaaaacagct tctcatgttt gatgaatatt gtgacttgtg accagttggt
    31801 aggagaacac cgtttagtgg agaggtggtg aaagaaacgt tcagccttga gcgatgacaa
    31861 aagctctcac aaacattgcg acgagtaacc gtaggagcaa taagaggcgg taaaaacccg
    31921 tctcttattt ttcgttttct ttcactgttt cccgttcaac tagtacaggt tcatagagaa
    31981 tatttggctg tgcctgtcct ttttcaatcg ttgtgacgat ccaccgtgcg gcatcttgtc
    32041 ccaacttttc ttttggatgc gtcaatgttg tcaatttttt attccctaaa tggcttaaag
    32101 aagaatcgtc tgttcctacg atcgataggt catctggaat atgatagcca ttttctgtca
    32161 acttatctag cattaaacta gctatttgat cgttatagca aatgatgcca tctaaagctt
    32221 gttcttcttt caaccgttgt actgtttttt cagcgattgt ttctcgtgtt tccgtggtga
    32281 aagtaatgat atcttccgtc aaaaaacgca cattatactg ttcacacgcg ctgataaaac
    32341 ctttcatcct gtattttcct tgaagatcgt cgatttttgt gatgaacaac agacgctgat
    32401 gtcctttttt tatcaaatat tctgctgcca taaaaccgac tttcacatca tctaaacaaa
    32461 tataagaggc atttaactct tcataagcag cattgatcat caccatcgga atgccttgtt
    32521 ctctcaaagc aacataaagt gctaagtttg ggttatactg attacttttc gtaggctcaa
    32581 cgatcaaacc atctacccca taagcaatca ttttcttcaa acactctttc tccaattcgt
    32641 gatcgttatt cgtactggct aaaagcaatg aatacccaga ttctcgtaat tcattttcga
    32701 ttcctcggat aatcgacgga aaaatataat cagagagata tgtggtgatg acaccgatcg
    32761 tttttgttct gttcaatggt gttttttctt tcttatagct gtcatctaca tatgtgcccg
    32821 accctttttc tttacgcaga tagccctcat ttaccaacac agcgattgct tgccgaatcg
    32881 tatgtctgct gacgtcatat atttcttgca gcttgaattc tgagggaatg gttgtattta
    32941 cttcgtattc ctcacgaagg atcttttgtt ttatctcttc tgcaatatct tgatattttg
    33001 ccatttgcct tgcccctttt tatttgtacg tacatatatc ataacgagtc tgcaaggttt
    33061 attcaagcaa aaatcaaggt ttcttgtgct cataaggcat acggaacagg ttcaccaaat
    33121 tctggaaatc cttgctcatt ccacgtaaag cattgcgcgt tcgcgtgccg attaggattg
    33181 tctaacggat cccctttttg gttcttctct ggtcgagcat gatagatcaa aatatcaaag
    33241 cacccgtctt ctgaaaccgt aaacgagttg tgaccaggac caaattgctt atttttttca
    33301 gaagaaacga atactggctc cgataattta tgccatgatt cttttaataa aagattcttg
    33361 tgctcatcag cccacagcag gcccatggca tagttttcat ctgttgcact accagaatac
    33421 gtaatgaata ttttcccatt gcgagtaagg actgctggac cttcatttac tgaaaaaccc
    33481 cgtttttccc attcatactc aggtatagaa aggaggatct gcttcccctt taacgtccaa
    33541 ggattatcca tttcggatag ataaagatta gaattcccag taatttcagg atcttgttgg
    33601 gcccacacat aatatagctt tccttgagaa gagaaagttg ttccgtctaa actgaaagtt
    33661 tcccttttcg tttttatttg tcccttttct atccaatctg tattcattgg atctatatgc
    33721 tgattttcaa gtacgaacat gcggtgatga tgagattcat cacgtatagt tttatgattg
    33781 cttgcagcaa agtaaatata ccattttcca ttgatataat ggacttcagg tgcccagatc
    33841 aattcactca tcggtccttc ttcatgagcc tgccatacaa tcaggcttgc agcatgttct
    33901 aattcattca gtgactttgc ccttcttaat tcaatcactt ggtagcctgg aaccgatcct
    33961 gtaaaataat aataaccatc tgtatgttta tatacccaag gatctgcccg ttgccgaacg
    34021 ataggattat tataaagtgt cttcatcata acaaatttcc tcacattttc tttattctaa
    34081 ttacattcca agagtatttt tctatcccta catttaattc gtcatgtttt tctattgaaa
    34141 aatagtttat ttcaggtttt attttttctg gttcttcacg tgtatttttg tccaacagat
    34201 cgctactggt taatgtgtag tgctctcctt gatctagcaa attgaaatca tgttcaatcg
    34261 ttatcttttg ttgttcattt gtgcgattga tcaagaaaat agtcagctga ttgccgttat
    34321 ctacgatcac actgtccaca taaggaacat tttttatctt ttcacaatca taaacagggc
    34381 aaatgatttg ttgtttcaat acttgacctt tagcatattt cgataagtgt tgcatcacat
    34441 aataagttgg attcctccat gcttgtcctt tagcatctgt cagtattaaa ggtattgtgt
    34501 ttactaacaa tgattgacaa ctaatcttta cgcgatctgc gtgtcgtaaa atagccagcc
    34561 ctaatgaacc aaataataat gtatcttcca tagtaaagta acaccaatcg atgggactac
    34621 cgacttcaaa atccctatgt cttttttccg gttgggcatg tacattccat tcatcaaacg
    34681 ctagatacat tgttttctca cttctcaaat atccttttac ataatcacaa gtagcaacga
    34741 tttcttcaat ttgacgatcg aatcgataag cttttgcaag gtatgtcgct gtatcatcac
    34801 gctcattttt aggaggttta gtcaagtctt cttcatcata cttatcaata tagttatgca
    34861 aagctaaata atctacattc tcataagctt cttctaatgc gatacgatcc cattcagggt
    34921 acgatggtaa acgtggcgta gagcttccaa ctaagattgt ttcaattgaa tcatccacta
    34981 acttcatgac ttttgaggct tcatttgcta acctaccata ttcataagca ctcttagccc
    35041 ccacttgcca tggaccgtct agctcattcc ctaaacacca taatttaacg ccaaaaggct
    35101 ttttttgccc attttttatt cttaaatcac tccagaaggt tccttttgga aaattacaat
    35161 attctactaa attttttgct tcatcaattc cttttgttcc taaattcacc gtgaatatcg
    35221 gtttcgcttg tacatgactg atccatttaa aaaattcgtg gagtccaaaa gaatttggtt
    35281 ctatacctcg ccatgctaaa tcaatttttt ttggtcgttg ttctttccta cctacagtat
    35341 ccacccaatc ataaccagat gtaaagtttc ctccaggata gcgaatgaca cctaattcta
    35401 attcctttac ataagagagg acatcttgtc taaacccgtc accatcagct gtttcatggc
    35461 ccggttcata aatccctgta tagacgacac ttcccatatg ctcaacaaat gagccaaaca
    35521 gccgagaact gatctctcct accgtaaaat cctctctgat ttttactttc acttcttcgt
    35581 ccaacagctt tccctacctt tcatttccta tagtcaagat aatagataca agtttccata
    35641 cgccaccagc aaagataaaa ataaaaggag ggaaaagaaa tatgagcagt acaaatccta
    35701 ataacaatac gcaacaaaga atatttctca aaaaatcaaa aaatactttc catgcacttt
    35761 gttcaaaaat ttgtaaaaat gtgatatctt tgttttcaga aatcacatat atccaggata
    35821 aaagaaacat ggaataaata agtaaaacta catatccact aattgaaagt acgattgtct
    35881 gttcttctaa taattgatta acaagaaaat aaataatgat cgttaacaga ctaaggaaca
    35941 agttcttacc aaaatgttgc tttattcctt gaaagaaaaa ataaatgata tttcctgatc
    36001 cctcaccttt ccattgcgcc attacataaa aaacagcaca tgtagcaggc acgatagtaa
    36061 taacaggcaa acacacgatt acccagagaa tacctaataa gatgtaatca gaaaccacaa
    36121 tcaactgtcc ataaaattta gtctcaacta ttttcatctt cctcactctt tcattccaga
    36181 catagctgtt ccttgaacaa aatatttctg tgcaaaaata taaaccacta gtaatggcag
    36241 cattgaaata accgttccag ccattacagg tccataaaaa gaaatgttct gccctgtaaa
    36301 taaagctaac cctgctggta aagttctcat ttttgaacta cttgtcaata tcaatggata
    36361 aagcaagtca ttccaactat tcatcagcgt gaaaatccct aatgataaaa gagtaggttt
    36421 gctcaatggc aacatgattt tcaaaaaaat attaaactca cttaatccat caatgcgtgc
    36481 tgcctcttcc aaagaatctg gtaaagtaat aaaaaaggaa cgcatcatga agataccaaa
    36541 tgcagttgtc atacgaggaa tgatcaaacc tgcataagta tcaagcatgc caagcaaatt
    36601 gacttcaata aatagaggaa tcatgaagac ttggaatggg atcatcattg tcagcaaaac
    36661 gaaatagaac aaaactgatt ttcctttgaa acgcatacga gcaaatgcat accccgcaag
    36721 agaatcaaag aatacagaag tgatcactgt ccctccagca aaaatcactg tatttttgat
    36781 gtaatctaat aaagggatcg ttgtccaaac cttaatatag ttatctaggg tatagctatc
    36841 tgcaaatatt ctaggcggaa atgagataat gtctttttcg tgtttaaaag aagaaataat
    36901 cagccacaaa aaaggaaacg caataaccaa caatataact agcatactaa tcgttcgaat
    36961 gattttttta ccactaactg cttttcgact attcacagta actgtaatcg agctttctgg
    37021 tgccatacag gtttccctcc tatttcattt gtttttcttt ccgtgacatg tacacattag
    37081 tggagatagc tatagttgca acaatgataa acaaaaccga agacagtgct gatgcatagc
    37141 ccaattcata aggtgctgta aatcctcgac tgtaaatata ttgaacagct gtttctgtct
    37201 taaattgtgg gccaccagcg gttgcaacat atacttggtc aaacacttgc atcgaaccaa
    37261 tcagattcgt taaaatacaa aaacctaagg agggaacgat ctgaggtagc gtaatgttaa
    37321 aaaactgctg tcgatttgtt gcaccatcca tttgagcaga ttcatacaaa gagggagaga
    37381 ttccctgaat agcagtcaag agaatcgtca tagtaacacc aaaattcttc caaacagtca
    37441 tgatggctac cgtcggcata gcaagggttg gatcgcgaaa aaattgaggg atcggcaagc
    37501 cgacttgtga aaacaagtaa ggaatcgccc ctacattcgg atcaagcaac atagaaaaaa
    37561 taatcgcaat tgacgtcaat gaacaaataa ctggaatgta aaatgtcgaa cgacaaaaac
    37621 gattgaatct ggtattttta gataatgcta ctgctgctaa taaaccaacg ataatttgtg
    37681 ttggcgtttc gagtacggtg aaatataaag aatgaatcaa cgaattaact gctcgttcat
    37741 cactaaatat acgtgtgaaa ttttcaaaac ttacaaaatt tcttgatgta aagaaaatat
    37801 tcaaatctgt aaaactaata gcaaatgttc ctaaaagcgg tatgacagta aataataata
    37861 aaactaaaaa agatggagtt aaaaacaaat acgcagcacg atcaggacga ttccaatatt
    37921 tggtgaatcc gttcatatag tccacctcat ctttcatttt gataaaatat acaatcttgg
    37981 tttaaacgaa tctgaataag atatccgaac aatcgctgtg atttttgcaa taacacctat
    38041 ctgcaaatac taggtcgatc attcagatat ctgtatcttg ccaacagtct atcctgtata
    38101 aagttctgta ctatttttgc agtaattgat tgatggcatc atctgcatcg ttcatgagtg
    38161 tctcaggatc ttctcctgct aataattttt caatcagtgg attgatgata tcgttattga
    38221 tcgcagagat ttgggtaaaa cctggtaaat atggctctgc atagtccatt tgtttcgata
    38281 attcagatac aataggatca ttttttactt cttcatcttt tgctactgaa tccaagtagg
    38341 caggaaaacc attttcgata ctccattttt taccaatttc agttgtattc caatatttca
    38401 aaaactcata gacagcttct tttttattat tatctgtact tgccggaatg gcatatccta
    38461 cgccatctaa aatagctgtt tttttgtctg aatcactccc ttgtggtaag gtagtcacat
    38521 tataatcaat ctcgtttgct ttcaatccgt tatttaacca tggcccattg atttcaatgg
    38581 ctagttgacc agcattcatt aaattgtcca tttcggcgcc agaaatcgat tctggaccag
    38641 tcttgtctac atggatcatt ttttggatcg cttttaacgt tttaaggttc tcaggagacg
    38701 caaattcaga cttagtataa tcatcgttca ctaatttacc gccatttgct aacatccagt
    38761 tatataacgg tgcagttcca tccttattga agacaaaacc tgatacattt ttactactat
    38821 cagttaattt tggtgccatt tctgtcaatt cttcccaagt agtaggtggt tgttcagtat
    38881 ctaatccagc ttgtttaaac aaatcattat tccaatacat atacatgcct tgtacctgca
    38941 tggggacaaa atactgttta ccttctactt cacctaattc aatagccgtt tctttaaaat
    39001 cagatttatc tactccatca tattcccaga aatcgtctaa tggttgtacc gctccgtttt
    39061 ctacatactg tgcaaaatct ggataattta atgcaataaa gtcaggtgct tttttagaag
    39121 aaatcgctgt aggaagtttt tcattcaaat tggcccaagt catgatatcc atttcaattt
    39181 tgatgttttt atcatttgtt ttattaaatt cattaacgat ttcttgcaat atttcaccat
    39241 cagatgcagt aaacccattc caaaaagtca aagtcacttc tttatcagaa tcagattctg
    39301 ctgaacttcc gccaccgcaa gctcctaaaa tacctgttgc agctaccaac aaagagccaa
    39361 ttaaccattt tttacctttc attttttgtt cctccttcat ctgataaacg acatctgacc
    39421 acattccaag atagcggttt caataaacaa ttgattttcc catcttcagc ttttacattt
    39481 gttgaatgac ttggttttac ttgttcactt ttaggaagat tagttgcttt tgtatcaaat
    39541 ccactcattt cagaaaaatc aatcactccc tctaatgata attcagacaa gtgaacagtt
    39601 agattcattt cttcttctcc acggttaact gcaaatatga caagttcttt gtcatttatt
    39661 acggctatac tttctaaata cggcacttct tcaaaatctt ccgtcgcata gtttgctgaa
    39721 cgaatttgtg ggcttaacac ctctccttgt ccatacacag cagcttgcat aaatggataa
    39781 tagatcgtct gtctccaagc ttctccgttt gtttctgtcc tgattggtgc aataacgttt
    39841 accaattgag caagacaagc aatctttact ctatctgcat gtttcaataa agtgattaat
    39901 agacagccta atagtaatgc atcttcaaaa ttatagttat cttctagtaa aggaggagca
    39961 atctgccaag gcgctgcttg ctcatcttgt tccgctgaat gataccaaat attccattca
    40021 tcaaaggaca ggttgatctg cttcttacta tgtttcttcg ccttgactgt atcgcacatc
    40081 gctgtcactt cagaaatgaa ccgatccatg tctaaggaac gtgcaagata atgagaaaga
    40141 tcattttttt ggttgccata atattgatgc agagacaaat aatctatttg atcgtacgct
    40201 tgatcaagaa ctgttaactc ccaatcacca aatgtaggca tttgccgatt cgaacttccg
    40261 cataaaacca cctcaattga atcatctacg agtttcatcg ccttagccgt ttcctctgct
    40321 aaccgtccat attcttccgc tgttttatgt ccaatctgcc aaggaccgtc catttcattc
    40381 cctaaacacc atgtcttaat tccaaaaggg ctctcttgac cattcgaacg ccttaaatca
    40441 ctccaataag ttcctttagg aaaattacaa tattcgacta aattacgagc tgcatctact
    40501 ccacgagtac ccagattgac agccatattg atttcggccc ccacatgctg acaccagtta
    40561 taaaattcat gaatcccaac ttcattcgtt tcaattgtcc gccaagctaa atccaacctt
    40621 ctaggtctat ttgctatggg cccaatactg tcttcccaac gaaatcctga cacaaaattt
    40681 ccaccaggat atctaatcat gggaatgttc aattctttta ccaagtgaat gacgtcttgg
    40741 cgaaatccac ttttattagc tgatggatga tctggctgat aaatcccttc atatactgct
    40801 cgtcccatat gttcaataaa tgaactgtac aaccgcggat caactttgcc tacacgcttg
    40861 acactactaa tcaacattgt ggcttccata actgacctcc tttaatttat acatgaataa
    40921 tacacctcgt tgaaagcgtt gtcaataata taaatgttaa acttacttat tttttatcga
    40981 ttttaattaa ccaaaatatt aataatgatt aaaacaaata atttaatata acttttatta
    41041 acaaatatgt taatatataa gaaaaacctt ttaagaggtg atatatatgc aactacaaat
    41101 aaacgaagaa tccttaccgg tatatgaagc attagctagt aaaacaagga tcaagatcat
    41161 ccagctactt tccaaaaaaa agatgaatgt caaagatttg gcaaaagaac ttggagtgag
    41221 cagtgctatc acgacgatgc acgtgaaaaa gctagaggaa gcaaacatca tcaaaacaga
    41281 aaaagtagga caacaaaaaa tttcgagcct tcgtgtcgat aaaatcgata ttagttttcc
    41341 agaaaaaata ttcaatgctt ttgatacgaa agaaacatcg atacctattg gtcactatac
    41401 aaattatgca attgaaccaa catgcggttt agctacgatc catgatttta tcgggaaagt
    41461 ggatgaacca cgctatttta tggatcctcg aaggatggat gcccgcattc tctggtttac
    41521 cagcggattt gtcgagtatc aggcacctaa ttttttaaat acagaggata cattggagat
    41581 gttggaagtt tctgtggaaa tttcttctga atttcctttt tcaaatgaca attggccttc
    41641 agatatcact ttcagcctaa atggtgtgga gctaggaaca tggacgagcc ctggagattt
    41701 tgctgatatc cgcggtaaat atacgcctga ttggtatcca gataatctta atcaatacgg
    41761 cttgctgaag acgatccgaa ttaccaaaca cttgacgaat atgaatggag aacccttgtc
    41821 aaacatcacg atcaacgaca tccctaaaga gcaagacact tggcatttgc gaatcgaagt
    41881 gaaagacgat gccaaacatg tggggggctg tactttattt ggtaaaggat tcggtaatta
    41941 cgatcaggat atcaaattaa aagtttacta cagctgatat ttttcttttc tattttataa
    42001 aacaaaaaca gccaatccct atgaagggac ggctgttttt gctttattgt tatttccaga
    42061 aatcatccaa aacggaaatt ggcaaatgtc gtttatgttg tgtcttgttc caccatgctt
    42121 cgattttttc ttgtgcttga gcgggaactt cttttccttc tagataatca tcaatttcat
    42181 tatatgtgac acctagtgcg acttcatctg caatcattgg tttcccatct tcaagatctg
    42241 cagtcggaat ttttgtgtat aatttttccg gtgcattcaa tgcttgaagc aattgtttcc
    42301 cttgtcgttt gtccaaacgg aataatggca aaatgtcggc tccaccatca ccaaatttcg
    42361 tgaagaatcc agtaatgttt tctgcagcat gatctgtccc taatacggct cctgctcttt
    42421 ctccagctac ggcatattgc gtaatcatac gttgacgtgc tttgatattt cctttgttaa
    42481 aatcgctgat tcccacacct gcttcgctta atacttgtac ttgtgcatca acagctggtt
    42541 tgatatttac tcgcagacta acatcgggct ggataaagtc aagagcagct tttgcatctt
    42601 cttcatctgc ttgttcacca taaggaagac gaacagcgat aaattgatag ttttcatctt
    42661 ttgtttcttc acgcatttct tccatcgtta gttgcgccaa acggcctgct aaagtagaat
    42721 cttggccgcc gctgatacct agtacaaaag ttttaagaaa cggatatttt ttcaaatatg
    42781 ctttcataaa gtcaatactt ttacgtattt cttcttttgg atcaattgtt ggtttgacac
    42841 ctaattcatt gataatttct gtctgcaggc tcatttattc tcctcctatg cttctgtatg
    42901 aagttctgct acgctttttt tcattcgttc catgatagcc attttatggt tccagcattc
    42961 agtagacaga tcgactgggt atttttgcgg attcaagtct cgtttatact cttcccatag
    43021 tgaatctaga ttttcttttg tatattcttt gatttcgttt agtgcaggta attcgtaaac
    43081 tctttttcct tcgataaaga tatcttgtaa aactgggcga gctgtaaagt ctcgaacagt
    43141 tttattgata aacgtatgca ctggatggaa catgaagata gcttcttctt ctcgagggtc
    43201 ttcatcccat aatgtcacgt agtctccttc agatttccca tcaaaatttc gagtgatgcg
    43261 ccacacttgt tttttccctg gagtagttac tttttctgca ttgcttgata acttgatcgt
    43321 atccatcatt tttccttcat catcttcgat cgatactagc ttgaatactg caccaagggc
    43381 tggctgatca taggctgtga tcaattttgt cccaacaccc catacatcaa ttttagcttt
    43441 ttgcatcttt aaattcaaaa tcgttgcttc atccaagtca ttcgaagcat agatttttgc
    43501 ttccgtaaat ccggctgcat ctaattgttc ccgaacacgc ttagatatat aggccatatc
    43561 tccactgtcg atgcgtacgc cttggaagtt gattttgtca cctaattctt tcgccacgcg
    43621 aatcgcactt ggcacacctg atttcaacgt atcataagtg tcgactaaga acacgcagtc
    43681 tttatgggtt ttagcgtagg ccataaatgc ttcataatca tttccataag attggactaa
    43741 cgaatgagca tgggtcccgc tagctggtat cccaaatatc ttcccagcac gtacattgct
    43801 tgttgcatct gctccgccga tatatgccgc tctagttccc cagactgctg catccagttc
    43861 ttgtgcccgt ctagttccaa attccaatag tggatcttct ccgatgacag atttgatacg
    43921 agctgctttt gtcgcgatca acgtttggaa attgaccata ttcaacagtg ctgtttctac
    43981 taattggcag tgcgctaaag gcccttccac ttggatcaat ggttcatttg caaaaactag
    44041 ttctccttca cgagcagaac gaactgttgc tttaaattca aaattttgta aatactccaa
    44101 aaaaccttca ggatagactt ctaatccacg caagtaagca atatcggaat cattgaacgt
    44161 cagattctct aaataattga ctaaccgttc caatcctgca aaaaccgcgt atccgtggtt
    44221 aaaaggcatt tctcgaaagt aacattcaaa aacagcatgt aaatcagctc gacctaattc
    44281 ccagtatgtc tgcatcatat tgatttgata tagatccgta tgcagcgtca aactgtcgtc
    44341 tgggtaagta ttattcatct tgtattactc ctcttggcaa gttttcattt cctacccatt
    44401 ataacaaaaa aaattaatag gaaaagaaat cttccatttt ttctgaaaaa gcacatgttt
    44461 tacggatatt tcttgctaaa aatgaactcc atctgctaaa actatccttt cttattggta
    44521 tttaacctaa acgagaaaga atacttattt tattcaatgt atccgagaca cgaagaaatt
    44581 agcgttttct atttttctta cttgataaaa taagtagatt ttcctactac aatcgtagcc
    44641 gttaagatac cgatgattga acaaatataa ccaaatgtct ctacaacata atcaatggca
    44701 aaagtttttg aagcaataat acgtctttcc ttttttgctt cttatcccca aagaaacgga
    44761 tataacttat tatgtattga atttttttgg aaaggcgaat tatgacaaga tataccttgc
    44821 gttgaaaaaa ctcgcggaaa aaatatcaca cgtttttaaa aagatacaaa gtaagaaagc
    44881 agctgtcaat agaatggaaa caaaaatggc gagacatcaa tcagtctcgc cttgtttttt
    44941 attgtataca ttagatatgg agcaggttac cataaagatg attattagtc gttattaaat
    45001 agaatccttc gtattttttt gttctatcac tacaatatct ttatttggac gtttgaattg
    45061 tattccggca cgttctagct cctgtttcac tcctacatgg actttgacat cttctcgtgt
    45121 catttttaaa acgtgatgaa tcgcatagtc gatttcttgc cttgtgattt ttttcttcgt
    45181 attgatcatc acgacttgct ttttatcata tgtgtctacc aatagcagtt gtgttcgatc
    45241 taacgaaaat aatttgtaat aattaatagc cgtattccca aaaaagaatt ccactacttt
    45301 aggataaaat gtcttaatat ttaaattact attcacaggt gtttcgatta gcttcatctc
    45361 attcctcttt tctatatagt cgatttagat ccaatcttaa gttatgttac catctttcct
    45421 agcattaagg aatcggttac aattgaacct ttaaagacaa aaggatttac cttttatcac
    45481 ttaatagtat aacataattt catgagaaaa aaatacaatt ttttcttgac catctcgtta
    45541 cgtaaaggat tatgcttaca tttgtcagga ggaaaaataa tggagtacac aataaagaaa
    45601 atgtcagaga tatcaggagt cagtccgaga accttgcgct tttatgatga gattggactg
    45661 ctaaaaccag ctaggatcaa ttcttcaggt tatcggattt atggcaaaaa agaagtcgat
    45721 cgtctgcaac acatcttatt ttatcgaaca atggcattca agttggatga tatccaagag
    45781 gtcttagaca atccatcttt tgaccaccag aaagctttaa tcaagcatca gcaaatgttg
    45841 ctggaaaaac gggcacaaat cgataccctc ttgacaactg tccaacagac acttgatatg
    45901 tacgaaggag gaagaaaaat gagtgacaaa gaaaaatttg aagggtttaa acaacaaaag
    45961 ttgaaagaaa atgaagaatc ctatggacaa gagattcgac aaaaatacgg aaaaaaaaag
    46021 gttacagcat ccaatgaaaa atggatgaat atggataaag aaacgtatga agaaatgcaa
    46081 actgttgaaa agcagatgtt atttgatctc actctttact taaaagaacc agaagatgaa
    46141 agcttagcag accgtatctt ccaagctcat aagaagtggt tatcttacag ctggcctgaa
    46201 tatcagccaa aagcgcataa aggactagga atgatgtacg aagccgatga acggttcact
    46261 gcatattatg acgagcgaag tggtcatggt gcagcaaaag ctttaaatga aattatccaa
    46321 aaaaaggcgt aatcttttac atgatccaac aaaaaagtac acgtaaaatg acctaataac
    46381 aaaacagcag acacccaagt tagattttta tgctaacttt tggtgtctgc tccattcttt
    46441 cacaagtttt ttcatcctac tttaaatata ttcatcgttg aataaacgta taaggtattt
    46501 tcactttatc ctttttttct gtaagaaaca attgaaatgc catttctccg caagcttcca
    46561 gatggtggtc gatggttggg aaaccaagaa gctcgctgat cagcaaatta tcgcgtccaa
    46621 tcaccactgg tgtcttatct tgcagatagc tttgcaagat gcctgctgca accatatccg
    46681 aatttgtaaa gacaccatca actccaagct ctttaaaaaa gaagcctgct tgttttcctt
    46741 tctctgcatc tatacaatcc caaaaaacaa gtgattcatc aaaagatgga aaaagttgat
    46801 gacatagctg caaagtgatt tttgagttat aacttagcct gccacttcgc cccaacgtga
    46861 tgcccaattg ctttatacct tgctgcttca aataagtaag accttctgta attgattgtt
    46921 ctcgatcaat aaagacacag cttgcctgtt ttcccttgat ttcttcacaa aaaactagag
    46981 gtccatattt ttgatatgcc aataaatcat ctagtgtatt ggcacgagac gtaatgatca
    47041 atccatcgaa agctttagct gcaaattctt caagatacct tcgctctttt tctttctgat
    47101 agcccgtcgg taaaagtgtc actttgtaat cataatcaaa cgctgcttca atgatcccac
    47161 tgatcaactg atcaaaatac ggatgattgg tataaggcag aatcactcca atgcttttcg
    47221 tttttccata acttaactgt ctggcatttg cattagggat ataatcgact tctttcatga
    47281 ccgcaagtat ttctttcctt ttttcctcat ctacatatgg atgtccattg attacacgtg
    47341 aaatagtaga aactgaataa cctgtgagct ttgcgatatc tcgaatgttt gccactgctg
    47401 cttcgccacc tatacaaaaa gattttcttg taaatagtct accacagctg gaccgcattc
    47461 aaaaaactga tacatttttc cttcatgcga aaaactgatc cgctgcccgc cccaatatat
    47521 atcttttgtc aaattttcga tttcattcat tggtatttct tttactatat tcctattcaa
    47581 cccttgaaaa ctgtacaacg ttaatacgga tcccttgaac tcgacaaacg cataaaaata
    47641 attgttcgtt gtgccatctg ttatctctac ataagtattc attgcaagcc ctccttttga
    47701 tactttgagt atagtgtatg aaacgcgtct catagcaagg agtttgtttt gccaaagaaa
    47761 agttttattc ctctttcatt attaccaaag tctcttattt taaattgaat acattataat
    47821 gatactgtaa atatatcttt attattagtg aatggatatt tacttttaat agataaaata
    47881 ttataactat taaaaataac tattttcatt ataattacac acaaaaaaga gcgtgagaca
    47941 aaagtaaaga ac
//
LOCUS       DARBEC010000010        47493 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.010, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000010 DARBEC010000000
VERSION     DARBEC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47493)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47493)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47493
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.010"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            23..211
                     /locus_tag="SCL80_000532"
     CDS             23..211
                     /locus_tag="SCL80_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011988843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine-rich KTR domain-containing protein"
                     /protein_id="HEG1106424.1"
                     /translation="MINYKWILCPICGNKTRLRIRVDTILKNFPLYCPKCKNETLINV
                     QKMNIITIKEPDAKTQSR"
     gene            complement(269..622)
                     /locus_tag="SCL80_000533"
     CDS             complement(269..622)
                     /locus_tag="SCL80_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001227349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106425.1"
                     /translation="MRKKEDKYDFRAVGLAIKEARMKRGLTREQVGTMIEIDPRYLTN
                     IENKGQHPSTQVLYDLVSLLHVSIDEFFLPTDNLIKSTRRLQVEKYMDSFTDKELSLM
                     EALAKGINEARNIEG"
     gene            1153..1575
                     /locus_tag="SCL80_000534"
     CDS             1153..1575
                     /locus_tag="SCL80_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006733729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1106426.1"
                     /translation="MKPSSFQETIENQFDYICKQVIEDERKDYFKHLDRLSAKEVSFS
                     DIDNYLVNSFSTVDTYVTDFQLFKLFGLRINVESDLLSEALRHLSEKKRNIILLHYFM
                     DMSDVEIAELLNLNRSTVYRHRISGLAMIKEFMKECKE"
     gene            1572..1802
                     /locus_tag="SCL80_000535"
     CDS             1572..1802
                     /locus_tag="SCL80_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106427.1"
                     /translation="MKTAYPRVPFPLIVKATDGDVEAINQIVKHYRGYTSKRSLRRMT
                     DEYGNSHMVIDETLRGRMETKLISKILSFEIK"
     gene            2300..2500
                     /locus_tag="SCL80_000536"
     CDS             2300..2500
                     /locus_tag="SCL80_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008475635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1106428.1"
                     /translation="MITVTKKDLVELGYGTSFSADIIKKAKELMITKGHIYYQSRKLD
                     RVPREAVEEILGIRFSNENRSE"
     gene            2527..3720
                     /locus_tag="SCL80_000537"
     CDS             2527..3720
                     /locus_tag="SCL80_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1106429.1"
                     /translation="MVKDPIKKAKNGTYYFRANLGYDSKGKKIQKYRSGFTTKKEARE
                     EYSKLLLMKPEELSENKDKMTFKHYIFEIFLPWYKTQVKLRTYENRLPTIKKHFSYFD
                     KMAVSDIEPIDVQNWQLKLSKKCKSSYVRAVQGLFSVAMDRAIVLGITTTNPSKIIGN
                     VKKQKSKIEFWTKEEFEKVISLIYKEDYYQHFLFISLWFLFMTGMRIGEATAIQWEDI
                     DFDSGVLTINKTLYYKNQSNYRFVEPKTKASVRHIVLDKCTLTYLSEWKEVQQNLIQT
                     DFVMSYNGIPTQKHTLSYAIERYAKLAGIHRIRIHGLRHSHASLLISMGENPLIIKDR
                     LGHEDIETTLGTYGHLYPNSNFEVANKLNGAISFKEAKQNYDTSPKNQFTVDYLRNKE
                     MKKVQ"
     gene            complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="SCL80_000538"
     CDS             complement(3788..5353)
                     /gene="guaA"
                     /locus_tag="SCL80_000538"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1106430.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVDARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(5412..6335)
                     /locus_tag="SCL80_000539"
     CDS             complement(5412..6335)
                     /locus_tag="SCL80_000539"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEG1106431.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            6531..7136
                     /locus_tag="SCL80_000540"
     CDS             6531..7136
                     /locus_tag="SCL80_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1106432.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            7444..8460
                     /locus_tag="SCL80_000541"
     CDS             7444..8460
                     /locus_tag="SCL80_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1106433.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            8601..9902
                     /locus_tag="SCL80_000542"
     CDS             8601..9902
                     /locus_tag="SCL80_000542"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEG1106434.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            9948..10610
                     /gene="deoC"
                     /locus_tag="SCL80_000543"
     CDS             9948..10610
                     /gene="deoC"
                     /locus_tag="SCL80_000543"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEG1106435.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            10631..11029
                     /locus_tag="SCL80_000544"
     CDS             10631..11029
                     /locus_tag="SCL80_000544"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEG1106436.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            11095..12177
                     /locus_tag="SCL80_000545"
     CDS             11095..12177
                     /locus_tag="SCL80_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1106437.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            12389..13951
                     /locus_tag="SCL80_000546"
     CDS             12389..13951
                     /locus_tag="SCL80_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106438.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            13944..15077
                     /locus_tag="SCL80_000547"
     CDS             13944..15077
                     /locus_tag="SCL80_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106439.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            15077..16033
                     /locus_tag="SCL80_000548"
     CDS             15077..16033
                     /locus_tag="SCL80_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106440.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            16132..17298
                     /gene="deoB"
                     /locus_tag="SCL80_000549"
     CDS             16132..17298
                     /gene="deoB"
                     /locus_tag="SCL80_000549"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEG1106441.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            17322..18140
                     /locus_tag="SCL80_000550"
     CDS             17322..18140
                     /locus_tag="SCL80_000550"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1106442.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            18157..18864
                     /gene="deoD"
                     /locus_tag="SCL80_000551"
     CDS             18157..18864
                     /gene="deoD"
                     /locus_tag="SCL80_000551"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEG1106443.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(19162..19848)
                     /gene="gpmA"
                     /locus_tag="SCL80_000552"
     CDS             complement(19162..19848)
                     /gene="gpmA"
                     /locus_tag="SCL80_000552"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1106444.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(20020..20601)
                     /locus_tag="SCL80_000553"
     CDS             complement(20020..20601)
                     /locus_tag="SCL80_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1106445.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(20734..21417)
                     /gene="rpiA"
                     /locus_tag="SCL80_000554"
     CDS             complement(20734..21417)
                     /gene="rpiA"
                     /locus_tag="SCL80_000554"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEG1106446.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            21736..22149
                     /gene="rpsL"
                     /locus_tag="SCL80_000555"
     CDS             21736..22149
                     /gene="rpsL"
                     /locus_tag="SCL80_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEG1106447.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            22233..22703
                     /gene="rpsG"
                     /locus_tag="SCL80_000556"
     CDS             22233..22703
                     /gene="rpsG"
                     /locus_tag="SCL80_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEG1106448.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            22797..24884
                     /gene="fusA"
                     /locus_tag="SCL80_000557"
     CDS             22797..24884
                     /gene="fusA"
                     /locus_tag="SCL80_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1106449.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            25056..26243
                     /gene="tuf"
                     /locus_tag="SCL80_000558"
     CDS             25056..26243
                     /gene="tuf"
                     /locus_tag="SCL80_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1106450.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            26650..26958
                     /gene="rpsJ"
                     /locus_tag="SCL80_000559"
     CDS             26650..26958
                     /gene="rpsJ"
                     /locus_tag="SCL80_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEG1106451.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            26989..27618
                     /gene="rplC"
                     /locus_tag="SCL80_000560"
     CDS             26989..27618
                     /gene="rplC"
                     /locus_tag="SCL80_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEG1106452.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            27646..28269
                     /gene="rplD"
                     /locus_tag="SCL80_000561"
     CDS             27646..28269
                     /gene="rplD"
                     /locus_tag="SCL80_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEG1106453.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            28269..28559
                     /gene="rplW"
                     /locus_tag="SCL80_000562"
     CDS             28269..28559
                     /gene="rplW"
                     /locus_tag="SCL80_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEG1106454.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            28598..29431
                     /gene="rplB"
                     /locus_tag="SCL80_000563"
     CDS             28598..29431
                     /gene="rplB"
                     /locus_tag="SCL80_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEG1106455.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            29474..29752
                     /gene="rpsS"
                     /locus_tag="SCL80_000564"
     CDS             29474..29752
                     /gene="rpsS"
                     /locus_tag="SCL80_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEG1106456.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            29775..30122
                     /gene="rplV"
                     /locus_tag="SCL80_000565"
     CDS             29775..30122
                     /gene="rplV"
                     /locus_tag="SCL80_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEG1106457.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            30136..30792
                     /gene="rpsC"
                     /locus_tag="SCL80_000566"
     CDS             30136..30792
                     /gene="rpsC"
                     /locus_tag="SCL80_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEG1106458.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            30795..31229
                     /gene="rplP"
                     /locus_tag="SCL80_000567"
     CDS             30795..31229
                     /gene="rplP"
                     /locus_tag="SCL80_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEG1106459.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            31219..31407
                     /gene="rpmC"
                     /locus_tag="SCL80_000568"
     CDS             31219..31407
                     /gene="rpmC"
                     /locus_tag="SCL80_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEG1106460.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            31432..31698
                     /gene="rpsQ"
                     /locus_tag="SCL80_000569"
     CDS             31432..31698
                     /gene="rpsQ"
                     /locus_tag="SCL80_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEG1106461.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            31756..32124
                     /gene="rplN"
                     /locus_tag="SCL80_000570"
     CDS             31756..32124
                     /gene="rplN"
                     /locus_tag="SCL80_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEG1106462.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            32161..32469
                     /gene="rplX"
                     /locus_tag="SCL80_000571"
     CDS             32161..32469
                     /gene="rplX"
                     /locus_tag="SCL80_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEG1106463.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            32497..33036
                     /gene="rplE"
                     /locus_tag="SCL80_000572"
     CDS             32497..33036
                     /gene="rplE"
                     /locus_tag="SCL80_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEG1106464.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            33055..33240
                     /locus_tag="SCL80_000573"
     CDS             33055..33240
                     /locus_tag="SCL80_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEG1106465.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            33276..33674
                     /gene="rpsH"
                     /locus_tag="SCL80_000574"
     CDS             33276..33674
                     /gene="rpsH"
                     /locus_tag="SCL80_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEG1106466.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            33706..34242
                     /gene="rplF"
                     /locus_tag="SCL80_000575"
     CDS             33706..34242
                     /gene="rplF"
                     /locus_tag="SCL80_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEG1106467.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            34403..34759
                     /gene="rplR"
                     /locus_tag="SCL80_000576"
     CDS             34403..34759
                     /gene="rplR"
                     /locus_tag="SCL80_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEG1106468.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            34780..35280
                     /gene="rpsE"
                     /locus_tag="SCL80_000577"
     CDS             34780..35280
                     /gene="rpsE"
                     /locus_tag="SCL80_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEG1106469.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            35295..35474
                     /gene="rpmD"
                     /locus_tag="SCL80_000578"
     CDS             35295..35474
                     /gene="rpmD"
                     /locus_tag="SCL80_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEG1106470.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            35518..35958
                     /gene="rplO"
                     /locus_tag="SCL80_000579"
     CDS             35518..35958
                     /gene="rplO"
                     /locus_tag="SCL80_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEG1106471.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            35958..37253
                     /gene="secY"
                     /locus_tag="SCL80_000580"
     CDS             35958..37253
                     /gene="secY"
                     /locus_tag="SCL80_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEG1106472.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            37315..37962
                     /locus_tag="SCL80_000581"
     CDS             37315..37962
                     /locus_tag="SCL80_000581"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEG1106473.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            38153..38371
                     /gene="infA"
                     /locus_tag="SCL80_000582"
     CDS             38153..38371
                     /gene="infA"
                     /locus_tag="SCL80_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEG1106474.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            38404..38520
                     /gene="rpmJ"
                     /locus_tag="SCL80_000583"
     CDS             38404..38520
                     /gene="rpmJ"
                     /locus_tag="SCL80_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEG1106475.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            38538..38903
                     /gene="rpsM"
                     /locus_tag="SCL80_000584"
     CDS             38538..38903
                     /gene="rpsM"
                     /locus_tag="SCL80_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEG1106476.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            38931..39320
                     /gene="rpsK"
                     /locus_tag="SCL80_000585"
     CDS             38931..39320
                     /gene="rpsK"
                     /locus_tag="SCL80_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEG1106477.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            39400..40338
                     /locus_tag="SCL80_000586"
     CDS             39400..40338
                     /locus_tag="SCL80_000586"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEG1106478.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            40366..40746
                     /gene="rplQ"
                     /locus_tag="SCL80_000587"
     CDS             40366..40746
                     /gene="rplQ"
                     /locus_tag="SCL80_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEG1106479.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            41018..41857
                     /locus_tag="SCL80_000588"
     CDS             41018..41857
                     /locus_tag="SCL80_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1106480.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMIFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            41833..42702
                     /locus_tag="SCL80_000589"
     CDS             41833..42702
                     /locus_tag="SCL80_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1106481.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            42692..43489
                     /locus_tag="SCL80_000590"
     CDS             42692..43489
                     /locus_tag="SCL80_000590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1106482.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            43692..44438
                     /gene="truA"
                     /locus_tag="SCL80_000591"
     CDS             43692..44438
                     /gene="truA"
                     /locus_tag="SCL80_000591"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1106483.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKIRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            complement(44771..47440)
                     /locus_tag="SCL80_000592"
     CDS             complement(44771..47440)
                     /locus_tag="SCL80_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEG1106484.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDGGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEISFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEQPRNTHRRRRRK"
BASE COUNT        15313 a         8742 c        10161 g        13277 t
ORIGIN      
        1 aagtggaaag gaggagattg ttattataaa ctacaagtgg atattgtgtc ctatttgtgg
       61 aaataaaaca agactacgaa tacgagtgga tactatactt aaaaatttcc ctttatactg
      121 ccccaaatgt aagaacgaaa ctttaattaa tgttcaaaaa atgaatataa taacaatcaa
      181 agagccagac gccaagacgc agagccgata atttgagaaa tgaaactctc atcttatcgg
      241 ctctttttgt ttatctgaat tttactgact agccttcaat atttcttgct tcattgatac
      301 cttttgctag tgcttccatt aaggatagtt ctttgtctgt aaagctatcc atgtattttt
      361 ctacctgtaa ccttcgagtg cttttaatca aattatctgt aggtaagaaa aattcatcaa
      421 tagatacatg gagtaatgat acaagatcat acagcacttg tgtacttggg tgttgtcctt
      481 tgttttctat atttgttaaa taacgtgggt caatttcaat cattgttccc acttgttcac
      541 gagtgagacc ccgtttcatt cgagcctctt taatcgctaa accaacggct ctaaaatcgt
      601 atttatcttc ttttttacgc atactaaatc acctctatac attttactgt tcctatataa
      661 ccataaacag gtatagaaaa acatgttata tagtttatga gttcctatag aacactgtgg
      721 aatttattaa tgcgatgaat ccaatttcaa atagaaaggt cgctgatttt atttacctat
      781 acaactttat aaaatttagt catactccta gaaagtagat aaaattggcg acttaatgga
      841 gggaaaagtc gccatgagaa agataatctg tcatctagtt ctcttaatta agttcttatc
      901 aaaaaaactt atccacctgt actggataga tatgaccatc aatgtggttt tgcagattac
      961 atagatagaa aaatcaaagc atatgaacag agaaaacaat ctgtttatgt gcttttttgg
     1021 tcacttaata aatatctttt aggatttctc ttcatttatg caacaaatcc cccgttcgtg
     1081 tcggggttta gtatgaaggg agataaagaa gcacatgcca ctccctttca cgacagaaag
     1141 ggggtgaaaa gcatgaaacc atcttctttt caagaaacca tagaaaatca gtttgactat
     1201 atctgcaaac aagttattga agatgaacga aaagattact tcaaacatct agacagacta
     1261 tcagcaaaag aagtctcgtt ttctgatata gataactacc ttgtcaactc tttttctact
     1321 gttgatacgt acgtcactga tttccaacta tttaaattat ttggattaag aatcaatgtt
     1381 gaaagtgatt tattaagtga agccctgcgt catctttcag agaaaaaacg gaacattatt
     1441 ttacttcatt actttatgga tatgagtgat gtggagattg cggagttact aaatttgaat
     1501 cgttcgactg tttatcgtca tcgaataagt ggactagcca tgattaaaga attcatgaag
     1561 gagtgtaaag aatgaaaaca gcttatccaa gggttccctt ccccctcatt gtaaaggcga
     1621 cagatggcga cgtggaagca attaatcaga ttgttaaaca ttatcgaggg tatacctcaa
     1681 aacgttcatt acgtcgcatg acagacgaat atggaaattc tcatatggtt attgatgaaa
     1741 ccctacgtgg cagaatggaa acgaaactca tctctaagat tttatcgttc gaaattaaat
     1801 agtcccattt tttcgtggaa gcgtgtcgaa tcgtatccac gcttcccgat ttgggaggtt
     1861 tgttattcca tcaaagcata ttgtgtgttc agtaggtttt gataggctaa caagccaaac
     1921 gttctttgac aaccaaataa aggcaatcga atacgtttcg gtaagaaaag agctgatgaa
     1981 ctaacacgcc atgacctaca aaattagtga gcgattcatg ttagtaattc gaaagtaatc
     2041 tttagcagga ttgctgccat gactttctta tcgtgataat gatactcccg tatagccata
     2101 gtccgagcgt tagaagcgtc acaggcaatg ggtacggcta catgagaacc atgcagaagt
     2161 gaaattcctg tgagctttgc tagagctgtt cgattgctaa gatattcttc tcttgtcagt
     2221 gtattattat aaaactattt gatataatta taggtgaata ttggagtttt cctagcctat
     2281 aattgggaaa ggagtaataa tgataacggt cacaaaaaag gatttagttg agctaggata
     2341 tggaacttct ttttcagcag acatcataaa aaaagcaaaa gaattgatga ttacaaaagg
     2401 gcatatctac tatcaatctc gaaagctaga tagagtccct cgtgaagctg ttgaagaaat
     2461 tctaggaatt cggttttcaa atgaaaacag gtctgagtag tttttgcaca gtaaggagta
     2521 aaaactatgg ttaaagaccc aataaaaaaa gcaaagaatg gaacctatta ttttcgtgca
     2581 aatctaggtt atgattctaa agggaaaaaa attcaaaaat atcgtagtgg attcactact
     2641 aaaaaagaag caagagaaga atattcgaaa ctgctattga tgaaaccaga agaattaagt
     2701 gagaataagg ataaaatgac gtttaaacat tatatctttg aaattttcct tccatggtat
     2761 aagacacagg taaagctcag aacatatgaa aatcgcttac caactattaa gaagcatttc
     2821 tcttattttg ataaaatggc tgtttccgat attgagccta ttgatgtgca aaattggcaa
     2881 ctaaaattat caaagaagtg taagtcttct tatgtaagag ctgttcaagg actattttca
     2941 gttgctatgg atcgagcaat tgtccttggg ataaccacca caaatccatc taagattatt
     3001 ggcaatgtga aaaagcaaaa gtctaaaatt gaattttgga caaaagaaga atttgaaaaa
     3061 gtaatctctc taatttacaa agaagattac tatcaacact ttttatttat ctctctgtgg
     3121 tttttattta tgactggaat gcgaattggt gaagctactg ccattcaatg ggaagatatt
     3181 gattttgact caggagtact aaccatcaac aaaacactct attacaagaa tcaatcgaat
     3241 tatagatttg ttgaaccaaa aacaaaggcg agcgttcgtc atattgtatt agataaatgc
     3301 acattaactt atttatccga atggaaggaa gtacaacaaa atctaattca gacagacttt
     3361 gtaatgagct ataacggtat tcctacccaa aaacatacac tatcatatgc tattgaacgc
     3421 tatgctaagc tggctggtat ccatcgaatc agaatccatg ggttacggca ttctcatgct
     3481 tccttgttaa tcagtatggg agaaaatcca cttatcatta aagatcgtct aggacacgaa
     3541 gatattgaga cgactttagg aacgtatgga catctttatc ctaacagtaa tttcgaggta
     3601 gctaacaaat taaatggagc catttccttt aaagaagcaa aacaaaatta tgatacttca
     3661 ccaaagaatc aatttactgt agactacttg cgaaacaaag aaatgaaaaa agtgcaataa
     3721 aagtgcagta aaaagataag aagagccaga aacccttggt ataagaggat ttctagctct
     3781 cctacaacta ttcccactcc actgttgctg gtggtttaga agtgatgtca tacacgatac
     3841 ggttgacgtg ggctacttcg tttacgatac gtacagagat tttttgcaag acttcccatg
     3901 ggatacgggc aaagtcagcg gtcattccgt cgattgaagt tacagcacgg ataccgactg
     3961 tgtagtcgta agtacgtccg tcacccataa caccgacaga gcggatccct ggaagaacag
     4021 tgaaatattg ccagatatca cggtcaagtc cagcagctgc gatttcttca cgcaagatag
     4081 catcagattc gcgtacgatt tctagcttgt cttcagtgat ttcgccaaga acacgaatac
     4141 caagaccagg accagggaat ggctgacgcc aaacgattga atcaggcatg cctaactgag
     4201 tccctaattc acggacttcg tctttgaata atgtgtttaa tggttcgatc aattcaaatt
     4261 gcatatcttc tggcagtcca cctacattat ggtgagattt gatcgtctgt gctgtttctg
     4321 taccagattc gataacgtca gtatacaacg ttccttgtgc taggaaagag acgccttctt
     4381 caccagcaag ttttgttgct tcgtcatcaa aaacatatac gaattcattc ccaatgattt
     4441 tacgtttttg ttctgggtca gatacacctt tcaacttgtc taagaagcga tctcttgcat
     4501 cgactttgat gatgttcaag ccgaattttc cgcctagact ttccattact tgttccgctt
     4561 cgcctttgcg tagcagaccg tggtcaacaa agatagatgt caattggtca ccgatcgctt
     4621 tttgtaaaag aaccccaacg acactagaat caacgccacc tgaaagacca agcaagactt
     4681 ttttgtcacc aacttgttca cggatttttt ctacttgcat atcgataaag ttatccattg
     4741 tccagttacc ttcacagtga caaacatcaa aggcaaagtg gcgcagcaat tcgattccgt
     4801 attcggaatg acgtacttct gcatggaatt ggataccata aaaattacgt gttgtgtctt
     4861 ggatagaagc aatcggacaa tctttgcttg ttccgactgt ttcaaagcct tcaggaactt
     4921 tagttaccaa gtcaccatgg ctcatccata ctgtttgttg ttttggtgtt tctttaaaca
     4981 agacagcttg atcgcttgtt acttctaaca aggctttccc atattcgcgg tttttagcag
     5041 gctctacgga accgccaagt ttgtacgtga tcaattgcat accatagcaa atccctaata
     5101 caggaatacc taaattgaag atctcaggat cgatcccaaa tgcattctca tcatatacac
     5161 tgtttggtcc gccagaaaga atgattccct ttggattcat cgcttttact tcttctgcag
     5221 taatgcggtg gctcaataat tcggaaaaga ctccaagatc acgaatacgt cttgtgatca
     5281 attggttata ctgactaccg tagtctaata cgataatctt ttcaacgttc gtcatatcgg
     5341 caacgtttgt caaatttatt acccctatcc ctttaaattt tatctcaaaa tagtcaaact
     5401 attttaaaat gctaatattt tcgcatgaaa atcgaatcga tgatatgatt ttcagtttta
     5461 tggagaatta tatcggctct tcctcgtgta ggcaagatat attcctgtaa atttttcaaa
     5521 ttcacatttt tccatacatc ttttgccatt ttaaaggctt cttcacgatc tccgatagca
     5581 aaacgataat aataattctc tggattttgg aaagccgtat ccagtaacgc accaaaacga
     5641 tccaaatacc atttttcgat cagcacggga tcggcatcga catagacaga aaaatcaaaa
     5701 aagtcgctga cgtagatttg ctgattcgca ggtaattgaa gcgtattgat cccctcaacg
     5761 atcaagatat caggctgctg gatcaattcg tacttgtctg gaatgatatc atacacatca
     5821 tgtgagtaaa caggtacttt tatctcttcc tgaccagatt ttacacggtt caagaaatcg
     5881 atcagcatct ccatatcata actttcagga aatcctttac ggtccatgat tccacgctct
     5941 tttaatatac gatttggata aagaaatccg tcagtcgtga tcagctgaac gtttcttcgt
     6001 ttgaaggtac gagacaaaat cgtctgcagc agtctggctg ttgtactttt tccaacggct
     6061 acacttccgg cgatcccgat aataaaaggc ggtacttcta cgtatttatg aagaaataaa
     6121 cctttactca agaacaaaga ttcaaattct ttcatataaa gataaatcag atgagcaaga
     6181 ggtacataga tatctcgaac atcctgcatg gaaatgcggt cattcaaact tttgatatta
     6241 tctagttcat cttgtgtcaa aggtgcttgc ccattacgat aaaattctcg ccattcttct
     6301 cttgccacac ggtagtagtt cattggatca ttcatattag cctcctgcgt tctcccatca
     6361 atggaactat tttaacacaa aataaacgca aaccccacca cttaagagag attgccgatc
     6421 tttttgtata atctttataa tagaaagaaa cggagtctta ttcttgactt gcgcacggat
     6481 acataaaagg atagactgtt gtcatgaaag aaatgagaag gatggaagac atgacaaatc
     6541 attactattc tgaacgccca gatacggcgc acgattttga acaatgggct tttgagttaa
     6601 aaggcagaac ctttcaattt gtaacagatt ctggtgtatt ttcacgtgaa acagtggatt
     6661 atggttcacg tgtcctgatt gatgctttcg attgggaaga gttacctgat gaaggaaaga
     6721 tcttagatgt gggatgcggg tatggaccga ttgggttagc tttggcattt gcgacacagc
     6781 ggtttgttga aatggtcgac attaatgaac gagcggtaag tctagcgcaa ggaaacgcaa
     6841 agcgtaacca aatcgaacat gttgatatcc atcaatcgaa tatctatgaa gcagttcatg
     6901 aagaaaccta tgcagctatc gtaagcaatc cgccgatccg tgctggaaaa aaagttgtac
     6961 atgaaatttt gacaggcgca tatcctcggt tgaagaaggg cggtacactg acaattgtga
     7021 tccagaagaa acaaggagcg ccgagtgcgc aaaagaaaat ggaagaaaca tttggtaatg
     7081 cagaaatcgt cacaaaagat aaaggatatt atatcatcaa aagtgtgaaa gaataactag
     7141 ctgaaaaata gtggagcgac gaaagagtgg aactgggcgt gcatgtacgg gtacgtatca
     7201 gttctttttt tgttgagaag tttttaaaaa caaagaacta ttttttcttc aaaaagaaat
     7261 agtaggaaca gagtcgttgt tttttacgat aaaataaata agatacagaa cttgtataga
     7321 aggctgccaa gtcggtagat aaaggaggtt tttatcaaaa aacatacttt ccttttttct
     7381 ctattctttt cttgggggtt ttggtacact gatagggaat ttagatagaa gggagccaat
     7441 aggatgaagc tgaccatcag agatatcgca gaaatggcag gcgtatcagt aacaactgtc
     7501 tcacagattt tgaataacaa aggaagcaga tttagtgata agacaagaaa aaaagtgttg
     7561 tcgatcgtga atgaacatca ttataagccc gattatttcg cttcaaatgt gatcaatcga
     7621 cattcaaaga ccatcggaat gatcgtccct gatgtcactg atttcttctt ctccaaactg
     7681 atcgaaggtg tagagagcta tttgaatcct ttgggctatg tgatcatgtt atgcaattct
     7741 cgtcatagcc aagaaaatga gatcaagtat ttacaagagc tttcccaccg ctcagtggac
     7801 ggaatcttgc tggccacacc aaatatttta cctgagaaat atgcattaga cagcggtttt
     7861 tatcaaaaaa tgccaatcat tttaatcgat cgcggattga atcgtcggga taacggacgg
     7921 cttatcgtaa aggaatatga gggcgcctat caagctgttt cgctgtttat tgaaaatggc
     7981 catacgaaga tcggcatgct gaaggaaacc actggatact atcaattaga agaacgtttc
     8041 aacggatatc gtcatgcgct gaaagatcat ggcctgcctt ttaatgggaa atttgttgaa
     8101 catggagagt tgactgtcca aggtggatat actgcatcaa aaaaattact caaacataaa
     8161 gatatcacgg ctattttttg cgggaatgat gccatggcga tcggatgcta tcaggcgatc
     8221 gatgaattgg gcaaaaaaat cccagaagat atctcagtca taggattcga tggattaaag
     8281 ctgtcagaat acatgattcc gcaattgacg acagtgcagc agccaagttt tgatattgga
     8341 ttttatgctg cacgtttcct gatcgatacg atcgaatttc ctcaaagaaa agttccgaac
     8401 aaagtgttcg aaacaaaatt aattattcgt gagagcgtaa aagtattgac aaagggctaa
     8461 ctcggcggta tattgcttat gtaagcattt acaaaggcta gttagtgctt tttttatttt
     8521 tagtaaacca gtttactaaa gtggtttacc tttcctaaaa ataatgtata ataccgaatg
     8581 aagacaagga ggatcaggca atgcgaatgg tggatctgat tgaaaaaaag agggatggaa
     8641 acgagctgtc gaaagaggaa atcgagtaca tcgtcacgaa ctatacgaat ggaaagattc
     8701 cagattatca agtcagtgca ttattgatgg caatttttta tcaagacatg acaaatgaag
     8761 aaatcacgaa cttaacattg gcgattgcta attctggtga tgtcatcgat ttgagttctc
     8821 ttgaaggaat aaaggtagat aagcattcga caggtggcgt tggtgacacg accacattga
     8881 ttttagcccc cctagtagct agtgtcggag tgacagtggc taaaatgtct ggacgcggtc
     8941 taggttacac aggtggtaca ctagacaagc tagaagcaat tcccggattt cagatcgagc
     9001 tttcggatga agaatttgta cgcattgtta atgaaagtaa agtagcggta attggtcaat
     9061 caggaaactt ggcaccggcg gacaaaaaat tgtatgcctt gcgtgacgtc acagcgaccg
     9121 ttgattctct tccgctgatt gccagttcga ttatgagtaa aaaaatcgcg gcaggagcag
     9181 atgcaatcgt tttggatgtg accactggag acggtgcttt catgaagaac atcgaagatg
     9241 caagacgtct ggcaaagacc atgacgagca ttgggaaact ggctaaccga gaaacagtgg
     9301 cggttatctc agatatgtca gaacctcttg gcgaagctat cggaaatagt ctagaagttg
     9361 tcgaggcaat cgaaacatta caaggaaatg gtccagaaga tctagtagaa atgtgctacg
     9421 ccttaggaag ccaaatggtt gttcttgctg gcaaagctaa gacaaccgat gaagcaagaa
     9481 cgttgcttca agaagctctg gaatcaggta aagcgttagc aaaattcaaa gaaatgatcc
     9541 aaaatcaagg gggagaccca gcgatcgtgg aacatccaga acgaattttg actgcacgct
     9601 acacaatgga gcttcctgct aaacaaagcg gagttgtgtc aaaaatcgtg gcaaatgaat
     9661 taggaattgc tgcaatgatg ctaggtgctg ggcgtaaaac caaagaagaa gatattgatc
     9721 acgctgttgg attgaaactg cataaaaaaa ttggtgacac tgtgacaaaa ggtgagtcac
     9781 tattgacgat ctatagtaac gacgaagaaa tcacttctgt cattgaatta ttatataaaa
     9841 atattgaaat tggtgaatcg gcaatgaagc caacgttgat tcatgacatc atcacagaat
     9901 aaaggaaaaa agcggacttt accgttttaa agaaaagggg aaaagaaatg gaactaaacc
     9961 gaatgatcga ccatactatt ttgaaagcag acgcatctaa agaagctgtg atgcaaatta
    10021 tcgaagaagc aaaaaaatac catttttatt ctgtctgcat caatccagca tgggttgcgc
    10081 tagcgaaaga acaattacaa ggtgagccag tagccgtatg tacagtaatc gggtttcctc
    10141 taggagcaaa cacacccgag acaaaagcat ttgaaacaac agatgccatc aataacggtg
    10201 cagatgaagt cgatatggtc atcaatatcg gtgctttgaa atcaaaggat gatcaacaag
    10261 tccaaaaaga tatcgaagca gttgttgaag cagcaaaaga tcgtgcgctt gtcaaagtta
    10321 ttattgaaac atcgttatta gatagagaag aaatcatccg agcttgcgaa atagcaaaag
    10381 ctgcaggcgc tgattttgtc aaaacatcaa ctggtttctc aacaggcgga gcaaaggtag
    10441 aggatgtccg tttgatgcgt gaaactgtag gaccagaaat gggtgtcaaa gcttccggcg
    10501 gtatccataa cgaagaagaa gcaatggcaa tgatcgaagc tggtgctaca agaatcggaa
    10561 caagcgcagg agtagcaatc gtatctggtt caactggtga aggctattaa ttttttcaaa
    10621 ggagaagctc atggcagaac tcaaacaaga atggatcaac acagctattg aagcgttaga
    10681 taaagcctat gttccttatt cccattttcc ggtaggcgct tgtctcgtaa cggaatcagg
    10741 caagatttat caaggcatca acattgagaa tgcatcattt ggattaacga attgtgccga
    10801 gcgcacagct tttttcaaag cagtatctga aggagaaaaa acatttacgc atctagtcgt
    10861 agcggggcac acccccgatc ctatctctcc ttgtggtgca tgtcgacaag taatggcaga
    10921 attctgttcc cctgatatgc cggttacgct agtaggagac aatggtgtga cgaaggcaac
    10981 gaccgttcgt gcactgctgc cttatgcatt tactgaaaaa gatctataac tcatgttcat
    11041 tcagtcagct gactgttgaa aaataaaaca attattttag gaggctttac agaaatgaaa
    11101 aaagcaaaat tatttggttt aggtgctgtc gcactagcag cagggttatt tttaggtgca
    11161 tgcggcaaca acggctctac tgattcatcc ggtggcaaaa gctcatcaga cacaacaact
    11221 gcagcattga tcacagacac aggtggtgta gacgaccgtt cattcaacca gtcagcttgg
    11281 gaaggactag aaaaatgggg gaaagatcaa ggactttcac gaggaaatga tggcttccaa
    11341 tatttccaat cttcaaatga atcagattac atcccaaata ttgaccaagc attgaatgct
    11401 ggattcaaaa cgatttttgg tattggttat aaactaaaac cagcgatcca agaacaagca
    11461 acaaacaata caggtacgaa ctttgttatt atcgatgatg tcatcgatgg attagataac
    11521 gttgtatctg caactttcaa agacaacgaa gcttcttatc ttgcaggtgt tgcagcagct
    11581 tatacgactg aaacaaacgt agtcggcttt atcggcggtg taaaaggtga agtaatcgat
    11641 cgttttgatg ctggattcaa agccggtgtt gatgcaggtg cgaaagaatt aggaaaagaa
    11701 atcaaagtat tgaatcaata tgctggtgac ttctctgctc cagataaagg acgttcaatt
    11761 gctcaaggta tgtatgcaca aaacgcagat atcatcttcc atgcttcagg cggtacaggt
    11821 aacggtgtat tccaagaagc aaaatcattg aacgaatcag gcgacaaaaa agtttgggtt
    11881 atcggtgttg accgcgacca atcagacgaa ggcgaataca cattaaacgg cgaaaagaaa
    11941 aacttcacat tgacatctac actgaaagca gttggaacag tagtagaaga tttggcacaa
    12001 aaatcagcgg acggtaaatt ccctggcggc gaacacactg tttatggatt gaaagaagac
    12061 ggtgttggtc taacagaagg acaactttct gacgaagcga aaaaagcagt tgatgaagca
    12121 aaagaaaaaa tcatttcagg tgacgtaaaa gttccagaaa ctcctgaaga aaactaaaac
    12181 catttctata gccttgttag acacatgttt gacaaggcta tggtttgatt agaacaagaa
    12241 ttgttcattt atccaagctg ccttgtgaca gtttggataa gggaacaact ccctgaagta
    12301 atgtttgttt caaaagaaga acgctcacta agcgtgaatc ttagtcaatc gttgccctag
    12361 atagaaatcg ttaggatgtg aaaaaaaggt ggttcaagaa aatattgtca tagaaatgca
    12421 gaatattacc aaagaatttg gaacattcaa ggcaaatgac aacatcaatt tacaagtaaa
    12481 ggcaggagaa attcatgcgt tgcttggaga aaacggtgct ggcaaatcta cattgatgaa
    12541 cgtgctttcc ggattattag agccgacatc agggaaaatt ttgatgcgtg ggaaagaagt
    12601 aaagatcaca agtccgacca aagccaacca attagggatt gggatggtcc atcagcactt
    12661 tatgcttgtt gatgccttta ctgtaacaga aaacatcgtg ttgggaagcg agcctagtcg
    12721 cgcagggatg cttgaccata aaaaagcacg aaaagagatc caaaaagttt ctgaacagta
    12781 tggattgtca gtcaacccag atgcttacgt tcgtgatatt tcagttggaa tggaacaacg
    12841 ggtagaaatt ttaaaaacac tttaccgagg agcagatgta ctgatttttg atgagccgac
    12901 agctgtattg acccctcaag aaattgatga attaatcgtg atcatgaagg aattagtcaa
    12961 agaaggcaag tcgatcattt tgattacgca taagttagat gaaatcaaag cagtagctga
    13021 ccgctgtaca gttatccgcc gtggaaaagg aatcggtaca gtcaacgtta aagacgtaac
    13081 ctcacaacaa ttagccgata tgatggttgg aagaacggtt tcattcaaaa cgatgaaaaa
    13141 agaagcgaag cctcaagaag tcgttttgtc tattgaaaat ctagtggtga aagaaaatcg
    13201 tggattagaa gccgtgaaaa acttgaactt agaggttcgt gctggcgaag tacttggtat
    13261 cgctggaatc gatggaaacg ggcagtcgga gttgatccaa gctttgactg gtttgcgaaa
    13321 ggcagaaagc ggacatatca agctaaaagg ggaagacatc actaataaaa aaccccgaaa
    13381 aatcactgaa catggtgtag gacacgtgcc agaagaccgt cataaatacg ggttagtcct
    13441 agatatgaca ttatctgaaa atatcgcact acaaacctat catcaaaaac cttacagtaa
    13501 aaacggcatg ctgaattatt cagtaatgaa tgaacatgcc agagaattga tcgaagaata
    13561 tgatgttcga acaacgaatg aacttgttcc cgcaaaagct ttatcaggcg gaaatcagca
    13621 aaaagcaatt atcgctcgtg aagtcgaccg agatcctgat ctgttgattg ttgcaaatcc
    13681 aactcgtgga ctggatgtag gtgcgatcga atttattcat aaacgtctga tcgaacaaag
    13741 ggacaaatac aaagcagtgt tactgatcag tttcgaatta gaagaaattt taaatgtttc
    13801 ggatcgtatt gctgttatcc atgaaggaga aattgtcggt atcgttgatc cgaaagaaac
    13861 atctgaaaat gaattaggtc tattgatggc gggctatacc ttagaagaag cacgaaaaga
    13921 gttagaaaag ggggctggcg cacatgaatg atcgcagcga aaaattccgt aatttattag
    13981 tgcctgtact ttcagttgtt ttaggattga tcttaggcgc gatattgatg gctgcttttg
    14041 gttttcagcc catccaaggt tattcttcta tgctagatgc agcccttggt tcgcaaagag
    14101 ggataggtga aacgttaagg caagcaacgc cgttgatttt tacagcatta ggtttttctg
    14161 tagctaactc agctgggttt ttcaatattg ggctttctgg acaagcactt tgcgggtgga
    14221 tcgtcagtgt ctgggtggca cttgcgtttc cagatctgcc aaaagttgtt ttgcttccgt
    14281 tatgcgtgat cttaggtgcc ttagctggtg cgatggcagc ggctgttcca ggtttacttc
    14341 gagcattttt tggaacaagt gaagtgatcg taacaatcat gatgaactat atcttattat
    14401 acgtaagcac atttgcttta catgaagtga tgcctgaaag ctatcgctca agtttggact
    14461 cttccaatat gatcagtcaa aatgcctcac tgcggttacc atggttaaca caagcttttg
    14521 gcggctctcg cgtaaactca ggattatttt tagcaatcat cgcgcttatt ttcgtttggc
    14581 tgctgatgaa gaaaacgact ttaggatttg aaatccgttc tgttggattg aatccttttg
    14641 cttctgagta tgcaggaatg agtagtaaac gtacgatcgt tttgtcgatg gtcatttctg
    14701 gcgctttggc agggcttggc ggtgtggttg aaggattagg aacgtatcaa aacttctttg
    14761 ttcaaacgac ttcgctatct attggatttg acggaatggc tgtttccttg cttggttctg
    14821 gatcagcaat cggtattctt ttatcagcga tcttgttcag tattttgaag atcggtggac
    14881 ttggcatgca gacaggagca ggtgttccgt ttgagatcgt caatgtatcg attgctttga
    14941 ttatcttctt tgtcgggatc aacttcctga ttcgtttcgt catggctaaa gtcttccaag
    15001 ggaaaaaaca agaagagatc gttgcgacaa tagaatcgaa accaacgaat caaaaccatg
    15061 aagggggcga actataatgt cgactatcga attaattgcc ttgatcctta cttctacact
    15121 ggtttattca acaccgctaa ttctaacgtc actaggcgga actttttctg aacgaagcgg
    15181 gatcgtaaac gtagggctag agggaatcat ggtcatgggt gcttttagtt ccatcgtttt
    15241 caacttgacg atgagtccat cacttggttc tatgacacca tggatcgcta ttttagtggg
    15301 tggcgtcgta ggtatcctat tttctctgat tcacgcagta gcaacagtca gtttgcgtgc
    15361 tgaccatatt atcagcggga cagtaattaa tctgatggca cctgctttag gtgttttctt
    15421 gatcaaagca tggtatggta aaggtcaaac tgataacatc acgcagaatc tggggtattt
    15481 ttctttccca ggattagcag atattccggt cattggacca attttcttta aaaatacatt
    15541 cttacctgct tattgtgcta ttttgattgc tgtcctagct tgggtgatca tcttcaagac
    15601 gaaattcggc ttgcgcctcc gttcagttgg tgaaaatcct caagctgccg acacattagg
    15661 tatcaatgta tatgctatgc gttattcagg cgtactgatt tctggcttgt taggcggtat
    15721 gggcggtgct gtttttgctc agtcgatttc cggtaacttt tcagctggta cgatcgttgg
    15781 acaagggttc atctccatgg ctgctatgat ctttggtaag tggaatcctt taggggcaat
    15841 gggtgcctca ttgttctttg gttttgctca aagcttgagt atcattggcg cacaattacc
    15901 agttatttct tcgattccgc cagtattgct gcaaattgct ccatatttgc tgacaatcat
    15961 tgtgctggtt gttttcttag ggaaagcttc tggtccaaaa gcaaacggga agaactatat
    16021 caaatcaaaa taatctttta gcgaataaaa agggtgggtt acgggaagtg tttttcgtct
    16081 cgcccttttt gttttaaagg caataaaaat aaaaagaaag aaggaataat catgtttaaa
    16141 cgcgtacatc tgatcgtcat ggattcagta ggaattggag aagcaccaga tgccgataag
    16201 ttcggtgatg ttggttctga tacattagga cacattgcga aagaagcagg attgaacgtt
    16261 ccacatttag agcagcttgg tttaggaacg atccgtcctc ttgaaggtgt ggaatcagtg
    16321 aaagatcatc aaggctatgc gacgaaatta gaagaagttt ctgtagggaa agatacgatg
    16381 acaggccact gggaaatcat gggattgaat atcaaaactc catttcgtgt gtttcctaat
    16441 ggattccctg atgatctgat caaacaaatc gaagaatttt ctggccgtaa agtcgtatgt
    16501 aacaaacctt atagcggaac agctgttatt gacgattacg gtgaacacca aatgaaaaca
    16561 ggcgatttaa ttgtatatac ttctgccgat cctgtccttc agatcgcggc acatgaagaa
    16621 atcattcctt tagaagaatt atatcggatt tgccaattcg ttcgtgatat aacaaaagat
    16681 gatccgtata tgattggccg aatcattgca cgtccgtacg tgggtgaacc aggagacttc
    16741 acacgaacaa gcaatcgcca tgattatgcg ttgaatccat tcggacacac agtccttgat
    16801 tctttgaaag aagcaggaaa ggacgtgatt gctgttggaa agatcaacga tattttcaac
    16861 ggacaaggaa tcactgaatc tgtccgcacg aaaagcaata tggatggcgt tgaccagctg
    16921 ctgaaagtta tgaaaaacga tttttcagga atcagcttta ccaatttagt agattttgat
    16981 gcattatacg gtcatcgtcg ggatgtagtc ggctatgcgc atgcgattga agaatttgac
    17041 ttacgaattc cagaattgct tgaagcgatg gaagaagacg acttgctaat gattacagcc
    17101 gatcacggaa acgatccgac gtttactggt accgatcata cgcgagaata tgtaccgtta
    17161 ttggtttaca gtaaaaaaat gaaagaacaa ggggcattgc ctcaaggata ttattcagat
    17221 atttcagcaa cgattgctga gaactttagt gtttcttcga ctgaaaatgg cgaaagcttc
    17281 ttgaaattat taaaataatc tttttaaata ggagaaagca aatgaccaag ttaagcgaga
    17341 tgttacaaga aacgacccag tttatccttg ataaaggagt aaaagaaatt gattttggtt
    17401 tgattctagg ttcaggatta ggtgaattag cagatgaaat cgaagatgct gtaatgattt
    17461 cttatgatga gatcccgttc tttcctgttt cgacagtagt tgggcatgca ggaaagctag
    17521 tttatgggtc tctggcaggc aagaaagtgt tagccatgca agggagattc cactactatg
    17581 aaggtcattc catgcaaaca gtgacctatc cagtacgtgt gatggccgga ctaggggccc
    17641 attcagtcat tgtgactaat gcatgtggtg gtgtcaacga atcatttgta ccaggagatt
    17701 tgatgctgat taccgatcat atcaatttta ctggtgaaaa tcctttgatc ggtccgaatg
    17761 aagatgcaat gggccctaga tttccagata tgagtcaagc ttatacgcct gactatcaag
    17821 caacagcaaa agcagcagca gaaaaactag gtttggatct taaagaaggc gtatatatgg
    17881 gctattctgg accaacttat gaaacgcctg cggagatccg aatgtctcgg acaataggag
    17941 ccgatgcagt agggatgtcg actgttccag aagtcatcgt agcagctcac agcggattaa
    18001 aagtgttagg aatctcttgt attaccaact tagcagcagg tatgcaagcg aatctgagcc
    18061 atgaagaagt tgtagaaaca actcagagag tcaaacaatc ttttaaagca ttggttaaag
    18121 aaacattgac attattgtaa taggaggaac aataaaatga gtgtgcatat tgaagcgaaa
    18181 gaaggcgaga tcgccgataa gattttactt ccgggagatc ctttgcgagc taaatatatt
    18241 gcggagactt ttttggaaga tccagtttgt tacaatcagg tacgcggtat gttaggcttt
    18301 accggcaaat ataaaggaga acgtatttct gttcaaggaa caggaatggg aatgccatca
    18361 gccaccatct atgcccatga attgatccag tcgtatggtg taaagaaatt gattcgagta
    18421 ggtacatgcg gtgcattgtc aaaagacgtc catgttcgtg atttagtact agcacaaggt
    18481 gcagcgacca gttcttcgat gatcgaaaaa aatttccaag cctttcattt tcctccaatc
    18541 agtgatttca atctattact gaaagcatat gagatagcta aagaaaaggg ctatacggtt
    18601 catgtaggaa atgttctttc ggaagattct ttctacaaag atgatttgac agagacgttc
    18661 caattagctg aacttggcgt tttaggagtg gaaatggaag cagcggctct ttattactta
    18721 ggcgcgaaat accatgtgca gacattaagt ttgatgaccg ttagcgacca tttgatcacc
    18781 ggtgaagaaa caacagctgc agaacgtcaa tctacgttca atgaaatgat cgaagtagga
    18841 ttggaaacag cgatagcaga ataagaagga aatgaaaaag ctgccctgca ttaagtaata
    18901 agaacatcca agcaaaaata gctcctcatg tttttgactt ggctggactt attattgcaa
    18961 atgtaagctt ttgaacaccg tttaccaaga ggttgtgaat ctgccgttta actctgagtc
    19021 acaaggaaca atttcaaaaa acagcttttc atgtttgatg aatattgtga cttgtgaccg
    19081 agaagttggc aggagaaaac cgtttatacg aaaaaaggga ctgtgacaaa tcgtgtcaca
    19141 gtcccttttt tatagtgttt tttataggta gtatttttta gcaactgtta agtcatcatt
    19201 taattcataa acaagtggtt gacctgttgg aatttcaaga tccatgatgt cttcgtcaga
    19261 gataccttcg atatgtttag ctagggcgcg taatgagtta ccatgtgcag ctactaaaac
    19321 agttttgttg tctagtaatg ctggagcaat ttcatcttgc cagaatggca aagcgcgttc
    19381 taatgtcact ttcaagtttt ctccaccagg aatatcgcgt ttgtctaaca ttgcgtaacg
    19441 acggtcattt gctgcagaac cttcgtcagt ttcttccatt aatggaggaa gagtatcgta
    19501 agaacgacgc cagatgtgca cttgttcgtc gccgtatttt tcagctgttt cttttttgtt
    19561 caaaccttgt aatttaccat agtgacgttc gtttaagcgc catgatttga tttgtggtac
    19621 ccaaagttga tctgaatatt ctaagatcaa gttacatgtt ttgattgcac gagttaaaac
    19681 agaagtgtaa gcaacatcaa attcgattcc agcttctttg attttgcgtc cgccttcttt
    19741 tgcttcttcg attccttctg gtgctagatt tacatcagcc caaccagtaa attggttcaa
    19801 tgcattccat tcactaagac cgtgacgaga aaaaactaat tttggcatgt ttagctctcc
    19861 ttttaagata ggtttatatt tctgaaattt tcagttctgt tttattgtac aattttttgg
    19921 aagagatttc catcgaaaaa ttagataaga ttctatgaga aaacaagtag agaatataaa
    19981 aaaacgaaca gtgcccacgc aactgttcgt ttttaggtat tagatgcata attgctcgat
    20041 ttctttttca ggtaaattga tatatttata acatgttcca actgaaacac cgcattttga
    20101 agaaataaat tggatcgttt cttttttttc ataatagaga cgtcggattt ttttgatcgt
    20161 ttttgcatcg atttttggtc ttccgccgat ttttcctttt ttacgagctt catctaatcc
    20221 aactaatgta cgttctttga tcaatgcgca ctccatgttt gctaggtttt ccattaattg
    20281 gaaatagatt tttcccattt cgcgcgtatc gattccttca tcaagactaa tcaagtgcag
    20341 cccgcgatct ttgaataatt gcgtgagttc tgttaattgc cgcgtagatt tgcctaatcg
    20401 atgaagttgg caaatgacta gtgcatcgcc ttgttgcatg tcagcgatca ctgactccaa
    20461 atgaacagca gtagaatcat tttgttccac ttcatatgtc tcatggaaaa tttcttgaca
    20521 accaaagtca gataattgat caacctgagt tgttaaatca acgtcattta tggttgtgcg
    20581 tgcataacca ataattttca tggtgattcc tttctagcag tgattttcta actggatata
    20641 acagtaaaaa tgtagcataa agtacgtcaa aaagatagtt ttttcgcaaa ttttccgctg
    20701 tcttttttta ttggatgatc aagcagtttt tctttattta cgacggattg cgtcaagaat
    20761 ctcaacgcct ttttctgagc cgacaactac tttagaaacc atgtttagga aaagcccatg
    20821 ttctactacc ccaacaagtt catctaaata ggtagctaag ctttctggct gttcgatgac
    20881 ttctagatgc agatcaatga tgtaatgacc accatctgtg gttaagactt ctccgttttc
    20941 gtctgtacgc aagacgggtt ggaatctttt ttcttcaaaa atatgaagta gctgttggga
    21001 gccataaggg atcacttcaa ctgggagtgg gaacttccca agtttatcga ctaatttaga
    21061 agaatcaacg atccaaatcg tttcttttga gtacgtagcg actattttct cgaaaagcaa
    21121 agcagcgccg ccgcctttga tcccttgaaa atcagcactg atttcatcag caccgtctat
    21181 tgtcaaatcg actacgggga cttcatcaat actctttaac ggtattccta atttttccgc
    21241 ttgttctttt gtcgcatttg aagtagtaac tcctgtgata cgtaaacctt cttcacgcat
    21301 acggcgacca atctcttcta ccatataata ggcggttgaa ccggtcccga gacctacgat
    21361 catcccgtcc tttacgaatt tcgcggcttc aatgccagct aattgtttta aattcatctt
    21421 tgcgtccacc tttttttagt ttgagcatcg tgctttcctc actattttag cgtgatttac
    21481 ggtaaaaaga taccattctt ttttattcca ataaagcttt ttcaaaaaaa attgcatctc
    21541 ttgttgacat gttttttcat tcgtgatatt ctaacaaagg tgctttatct acaggtttct
    21601 gtgaagaaac gtgctgactg tttatcaaag catatttgat aaggatgcag acattgcatt
    21661 tattttttat cacaaaaaaa gaaccacctg gatgtgtgga actagacgca aatcgtggga
    21721 aggaggaaac aaggaatgcc tacaattaac caattagtac gtaaacctcg taaatcaaag
    21781 gtggaaaaat ctaattcacc tgctttgaac aaaggatata acagctttaa gaaaactcaa
    21841 actaacgtaa actcacctca aaaacgtggg gtagcaactc gtgtgggtac aatgacacct
    21901 aaaaaaccga actcagcttt acgtaaatat gcacgtgttc gtttgtcaaa cttaattgaa
    21961 gttactgctt atatcccagg gattggtcat aacttacaag aacacagtgt tgtattatta
    22021 cgcggtggtc gtgtaaaaga cttaccaggg gtacgttacc atatcgttcg cggtgcttta
    22081 gatactgctg gtgttaacga ccgtaaacaa agccgttcta aatacggaac taaaaaacct
    22141 aaagcttaat tttaattatt aaacaaatga aacttgcttc tactatatag aagtaattac
    22201 tatgaacaat atttcggaag gaggagttac ggatgccacg taaaggtcct gttgcaaaac
    22261 gtgatgtttt accagatcct atttataact caaaattagt aactcgcttg atcaaccgtg
    22321 taatggttga cggaaaacgc gggattgcag ctaacattat ctataatgcc tttgacttga
    22381 tcaaagaatc tacaggtaac gatccattag aagtgtttga acaagcaatg aaaaacgttc
    22441 aacctgttct tgaagttaaa gcacgtcgtg ttgggggttc aaactaccaa gtaccagttg
    22501 aagttcgccc tgaacgccgc gttactctag gtcttcgttg ggtagtaaac tacgctcgct
    22561 tgcgcggtga acacacaatg gaagaacgct tagcaaaaga aatcatggat gctgcaaaca
    22621 acacaggtgc ttctgttaaa aaacgtgaag acactcataa aatggctgat gccaaccgtg
    22681 cgtttgctca ctatcgctgg taagattctc tctgtttggt cggcttttat aagtcgacct
    22741 cagatagtgt ccttattttt agcaactaga tagtaaagag aggagaaaca aggagaatga
    22801 ccagagaatt ttctttacaa aacactcgta atatcggtat catggcccac atcgatgctg
    22861 gtaaaacgac tgctactgaa cgtatcttgt actatactgg taaaatccat aaaattggtg
    22921 aaactcatga aggagcttca caaatggact ggatggaaca agaacaagaa cgtggtatta
    22981 cgatcacttc tgctgctact acagcacaat ggaagggtca ccgaatcaac atcatcgaca
    23041 caccaggaca cgtagacttc acagttgaag ttgaacgttc attgcgtgta cttgatggtg
    23101 ctgtaactgt tcttgatgca caatcaggtg ttgaacctca aactgaaaca gtttggcgtc
    23161 aagcaacaac ttatggtgta cctcgtatcg tatttgcaaa caaaatggac aagatcggtg
    23221 ctgacttctt atattcagta tcaactttac atgaccgttt gcaagcaaac gctcacccaa
    23281 tccaattacc aattggtgcg gaagacgact tcacaggaat tatcgacctt gtaacaatga
    23341 aggctgaaat gtatacaaac gatcttggaa cagagatcga agaaacagaa attcctgaag
    23401 aataccgcga attagctgaa gaatggcgcg aaaaattggt tgaagctgta gctgaaactg
    23461 atgaagaact tactttgaaa tacttagaag gtgaagaaat cactgaagct gagttaaaag
    23521 aaggtatccg ccgcgcaaca gttaacgtag aattctaccc agtactatgt ggttcagcat
    23581 ttaagaacaa aggggttcaa ttactattgg acgctgttct tgattacttg ccatcaccgt
    23641 tagatatccc agctatcaaa gggattgacc ctaaaactga tgaagaagta gaacgtcctg
    23701 cagacgacag cgctcctttc tcagctttag catttaaagt tatgactgac ccattcgtag
    23761 gtcgtttaac tttcttccgt gtgtactctg gtgttttaca atcaggttca tacgtacaaa
    23821 acgcaacaaa aggcaaacgt gaacgtgttg gtcgtatcct acagatgcat gccaactctc
    23881 gttcagaaat ctctgaagtt tacgctggag atatcgctgc tgctgttggt ttgaaagaca
    23941 caactactgg ggacacacta tgtgatgaaa agagtctagt tattcttgaa tccatggaat
    24001 tcccagaacc agttatccaa gtcgctattg aacctaaatc aaaagctgac caagataaaa
    24061 tgggtgtggc tttacaaaaa ctatctgaag aagatcctac tttccgtgct gaaacaaacg
    24121 tggaaacagg cgaaacaatc atcgctggta tgggtgaact tcacttagat atcatcgttg
    24181 accgtatgcg tcgtgaattc aaagttgaag ctaacgtagg tgcgcctcaa gtatcttacc
    24241 gtgaaacttt ccgtgctggc actcaagccg aaggtaagtt tgtacgtcag tctggtggta
    24301 aaggtcaata cggtcacgta tggattgaat ttacaccaaa tgaagaaggt gctggtttcg
    24361 aatttgaaaa tgcgatcgtc ggtggggttg ttcctcgtga atacattcca gctgttgaaa
    24421 ctggtttgaa agatgctatg gaaaatggtg tcttagctgg ctatccatta gtagatatca
    24481 aagctaaact ttacgatggt tcataccatg atgtcgattc aaatgaaact gccttccgtg
    24541 ttgctgcttc tatggcatta cgtgcagcag ctaagaaagc taatccagta atccttgaac
    24601 caatcatggc tgtagaagtt gttatccctg aagattattt aggtgatgtt atgggtcacg
    24661 taactgctcg tcgtggacgt gttgaaggta tggaagctcg cgctggcggc caacaagttg
    24721 ttcgtgcgat ggttccactt gctgaaatgt ttggttatgc tacaactctt cgttctgcta
    24781 ctcaaggtcg tggtacattt acaatgacat ttgaccacta cgaagatgta ccaaaatcag
    24841 tacaagaaga aatcattaag aaaaacggcg gaaaagctga ataatttctt aaaaccggat
    24901 agtcctgcaa agagattatc caaagagact atatttttaa tatagtttcg tgtaaaataa
    24961 ttatcgatga tatgggcatg tggtgtacct tgtgtacatc atatacctat cagacaaata
    25021 aaaaatatat tgtattttta ggaggacatt ttaaaatggc aaaagaaaaa tttgaccgtt
    25081 ctaaaccaca cgttaacatt ggtactatcg gacacgttga ccatggtaaa actacattaa
    25141 cagctgcaat cacaactgta ctatctaaga aaaacggcgg ccaagctatg gcttacgatc
    25201 aaatcgatgg tgctccagaa gaacgcgaac gcggaatcac aatctcaaca gctcacgttg
    25261 agtatgaaac agatacacgc cactacgctc acgttgactg cccaggacac gcggattacg
    25321 ttaaaaacat gatcactggt gctgctcaaa tggacggagc tatcttggta gtttctgctg
    25381 ctgacggccc aatgcctcaa actcgtgaac acatcctatt gtctcgtcaa gttggtgttc
    25441 cttacatcgt tgtattcttg aacaaagtag acatggttga tgacgaagaa ttactagaat
    25501 tagttgaaat ggaagttcgt gacctattaa cagaatacga attccctggt gacgatgttc
    25561 ctgtagttgc tggatcagct ttgaaagctc tagaaggcga cgcttcatac gaagaaaaaa
    25621 ttcttgaatt aatggctgca gttgacgaat acatcccaac tccagaacgt gacaacgaca
    25681 aaccattcat gatgccagtt gaagacgtgt tctcaattac tggacgtggt actgttgcta
    25741 caggtcgtgt tgaacgtgga caagttcgcg ttggtgacga agttgaagtt gttggtattg
    25801 ctgaagaaac ttcaaaaaca acagttactg gtgttgaaat gttccgtaaa ttgttagact
    25861 acgctgaagc tggagacaac attggtgctt tactacgtgg tgttgcacgt gaagacatcc
    25921 aacgtggaca agttttagct aaaccaggta caatcacacc tcatacaaaa ttctctgcag
    25981 aagtatacgt gttgacaaaa gaagaaggtg gacgtcatac tccattcttc actaactacc
    26041 gtccacaatt ctacttccgt acaactgacg taacaggtgt tgttgaatta ccagaaggaa
    26101 ctgaaatggt tatgcctggc gacaacgtaa caatggaagt tgaattaatc cacccaatcg
    26161 ctatcgaaaa cggtactaaa ttctcaatcc gtgaaggcgg acgtacagtt ggtgccggtg
    26221 tcgttacaga aatcaaagct taattcactt aagttttaaa gtaaagcaac atttattcgg
    26281 acgtaatcgc tcggaacgaa ggtcttagga tctttgttcc gagctttttt atttattgca
    26341 ggatcaggaa aaaagacagg agtattataa atagaagaaa cacgaaataa aagagtcagt
    26401 attttctaaa tgaaacttta tgaaaacaaa atgtttttta caagaaaaag ccttgctttg
    26461 tgagtttata tatattataa tgacaaaggt gcgtttactt tagtgaattg cacagacgta
    26521 atcagaatga tttcgtcggc taagaaacga gaggttgcga cacgcccgga cgctttgcca
    26581 cgaacgagcg tgacggaaat tttcgtggag ctatgtctac ttttcaaaat aggcgaagga
    26641 gggaacaaga tggcaaaaca aaaaattcgt atccgtttaa aagcgtatga acaccgtatt
    26701 ttagatcaat cagcggataa aatcgtggaa actgcaaaaa gaactggagc tagcgtgtct
    26761 ggtccaattc cattaccaac tgaacgcagt ctttacacaa tcatccgtgc gactcataaa
    26821 tacaaagatt cacgcgaaca attcgaaatg cgtacacaca aacgtcttat cgacattgtg
    26881 aacccaacac caaaaactgt tgatgctttg atgaagctag acttaccatc tggtgtaaac
    26941 atcgaaatca aattataaaa ctaaaacaaa aataatggag gtgtactcat gaccaaagga
    27001 atcttaggga aaaaagtggg aatgacacaa atcttcactg aatctggtga attaattcca
    27061 gtaactgttg tagaagctac tccaaacgta gtactacaag tgaaaacaat ggaaacggat
    27121 ggctacgaag ctattcaagt tggttaccaa gatatgcgtg aagttttatc aaacaaacct
    27181 gcgaaaggtc atgttgcaaa agcaaacacg gctcctaagc gcttcattcg tgaattcaaa
    27241 aatgttgagc taggagaata tgaagtagga aaagaaatta cagtagatgt tttccaagca
    27301 ggagacatta ttgatgtaac aggtactacg aaaggtaaag gattccaagg ggttatcaaa
    27361 cgccacggac aaagccgcgg accaatggct cacggttctc gttaccatcg tcgtcctggg
    27421 tcaatgggtc cagttgcacc taaccgtgta tttaaaaaca aacgtcttgc tggccgtatg
    27481 ggtggcaacc gcgtaactat ccaaaacttg gaagttgtac gtgtcgatgc agaaagaaat
    27541 gttatcttga tcaaaggtaa tgttcctgga gctaaaaaat ctttaattac tatcaaatca
    27601 gctgtgaaag ctaaataatt gtaggaagaa aggaggaact aaggaatgcc gaatgtagca
    27661 ttattcaaac aagatggaag ccaaaacggt gaaatcacac ttaacgaaga aatcttcgga
    27721 attgaaccta atgaaagtgt tgtttacgat gcaatcgtaa tgcaacgtgc gtcattaaga
    27781 caaggaacac acgcggttaa aaaccgtagc gctgttcgcg gcggcggacg taaaccatgg
    27841 cgtcaaaaag gaacaggtcg tgcacgtcaa ggatctatcc gctcaccaca atggcgtgga
    27901 ggtggcgtag tctttggacc aacaccacgt tcttacagct acaaacttcc taaaaaagtt
    27961 cgtcgtttag caatgaaatc tgtattgtca gaaaaagttg ctgaaaacaa cttggtagca
    28021 atcgaaggct tgaacttcga cgcaccaaaa acaaaagaat tcaaacaagt tcttgctaac
    28081 ttatctatcg actctaaagt attggttgtt ctagaaaaag gtaacgactt tgctgcttta
    28141 tcagcacgta acttaccaaa cgtttctgta gtaacttctg ataatgtgag tgtgctagac
    28201 gttgtatcaa atacaaaagt gttagcaaca caaactgctc ttactcaaat tgaggaggtg
    28261 cttgcataat gaacttacta gacgtaatca aacgcccagt gatcactgaa aaatcaatgc
    28321 ttgccatgga tgacaagaaa tacacttttg aagtggacac tcgcgcaaac aaaactttag
    28381 taaaacaagc ggtagaagct gcttttgacg ttaaagtcaa aaacgtgaac atcgtgaacg
    28441 tacgtgcgaa attcaaacgt atgggcaaat atgcaggata tacaaaaaaa cgtcgcaaag
    28501 cgatcgttac attgactgaa gattcaaaag aaatccaatt attcgaagct gctgaataag
    28561 cactaataga taactcatta aattaggagg gaaaaacgtg gcgattaaaa agtacaaacc
    28621 tacctcaaat ggtcgtcgta acatgacagg ttctgatttt gctgaaatca caacatcaac
    28681 acctgaaaaa tcattgttac aaccattgaa aaaccatgct ggacgtaaca acaacggccg
    28741 catcactgta cgtcatcaag gtggcggaca caaacgccaa tatcgcgtga tcgacttcaa
    28801 acgtaacaaa gataacgttg ttgcaactgt tcaaacgatc gaatatgatc caaaccgttc
    28861 agctaacatc gcgttagtac actatgaaga tggagtgaaa gcttacatct tagcacctaa
    28921 aggactagaa gtgggtatga aacttgtttc aggtccagaa gcagatatca aagtcggaaa
    28981 tgctcttcca ttagaaaaca ttccagttgg tactgttatc cacaacattg aaatgaaacc
    29041 tggtaaaggt ggacaattga tccgttctgc tggtacaagt gctcaagtac ttggtaaaga
    29101 aggcaaatac gtattgatcc gcttgaactc tggtgaagtt cgtatgatct tagctgcatg
    29161 ccgtgctaca atcggttctg taggtaacga acaacacgaa ttaatcaaca tcggtaaagc
    29221 tggacgctct cgttggatgc gtaaacgccc aactgtacgt ggtagcgtaa tgaaccctaa
    29281 tgatcaccca cacggtggtg gtgaaggtaa agctcctatc ggacgtaaag caccagtttc
    29341 tccttggggt caaccagctc ttggttacaa aactcgtaac aaaaaagcta aatcagacaa
    29401 acttattgtt cgtcgtcgca aaaataaata attactttga ccatgtgtcg cacattttga
    29461 gaggaggttc accatgggtc gtagtttaaa gaaaggacct tttgtcgatg agcatttaat
    29521 gaaaaaagtt gaagctcaag caggggttga aaagaaaaaa gttatcaaaa cttggtcacg
    29581 ccgttcaaca gttttcccaa gttttgttgg atataccatc gcagtttatg atggacgtaa
    29641 acatgtacca gtttacatcc aagaagacat ggtaggacac aaattaggtg aatttgcacc
    29701 tacaagaact tatcgtggac atgctgctga cgataagaaa acgaaacgct aatttgaggg
    29761 gaggataaat ccaaatggca gaacaaatca catcagctaa agcaactgca aaaacagttc
    29821 gcacttcacc tcgaaaagcc cgtttagtaa ttgaccttat cagaggtaaa agtgtcgcag
    29881 atgcaatttc aatcttgaaa ttcacaccaa acaaatctgc tggaatcatt gaaaaagtat
    29941 tgatgtcagc agttgcgaat gcagaaaata actttgactt agatgttgaa aacttggtag
    30001 taagcgaagc ttttgttaac gaaggaccta caatgaaacg tttccgtcct cgtgcaaaag
    30061 gatcagcttc tccaatcaac aaacgtacaa gtcatatcac ggtagtcgta tcagaaaaat
    30121 aaggagggat aatcagtggg tcaaaaagta catccaattg gaatgcgtgt aggcatcatc
    30181 cgcgactggg atgctaaatg gtatgctgaa aaagagtatg ctgagttctt acacgaagat
    30241 ttaagaatcc gtaaatttat ttcaactaaa cttgctgatg ctgctgtatc aacagttgaa
    30301 atcgaacgcg caacaggtcg cgtaaacatt tcaattcaca cagctaaacc aggtatggtt
    30361 attggtaaag gcggttctga agtcgaaagc ctaagaaaag aattaaacaa attaactggt
    30421 aaaagaatcc atatcaatat cgtggaaatc aaaaaaccag atctagatgc aaaattagta
    30481 ggtgaaggaa tcgcacgtca attagaaaac cgtgttgctt tccgtcgcgc tcaaaaacaa
    30541 gccatccaac gctcaatgcg tgctggcgct caagggatca aaactcaagt atctggtcgt
    30601 ctaaacggag cagatatcgc tcgttcagaa ggatactcag aaggaactgt tcctcttcat
    30661 actttacgtg cggacattga ttacgcatgg gaagaagcag atacaacata cggaaaacta
    30721 ggagttaaag tgtggatcta tcgtggagaa attcttccaa ctaaaaaaaa cactgagaaa
    30781 ggagggaaat aatcatgtta gtacctaaac gtgtaaaaca ccgtcgtgaa ttccgcggaa
    30841 aaatgcgcgg ggaagctaaa ggcggaaaag aagtagcatt cggtgaatac ggtttgcaag
    30901 ctgttgattc acattggatc acaaaccgcc aaatcgaagc tgctcgtatc gcaatgactc
    30961 gttacatgaa acgtggtggg aaagtatgga ttaaaatttt ccctcacaaa tcttatactg
    31021 ccaaagcaat tggggtacgt atgggttctg gtaaaggggc acctgaagga tgggttgcac
    31081 cagtaaaacg tggtaaaatc atgtttgaaa tcgcaggcgt tcctgaagaa gtagctcgtg
    31141 aagcgttacg tctagcttct cacaaattac caatgaaaac taagatcgta aaacgtgagg
    31201 aaatgggtgg tgaatcgaat gaaggttaaa gaaatcagag aattaaccac tgccgaaatg
    31261 cttgatcaag aaaaacaatt aaaagaagaa ttgtttaatc ttagattcca attagcaaca
    31321 ggtcaattag aaaacactgc acgtattaaa gaagtacgta aatcgattgc acgcatcaaa
    31381 actgtgttgc gtgaacaagc taagtaatta tggaaggagg ccattaagcg tatgactgaa
    31441 ggaagaaatc aacgcaaagt ttatcaaggt cgcgtggttt cagacaaaat ggataaaaca
    31501 attaccgttg ctgtcgaaac aaagaaaaac caccctatct atggcaaacg catgaactat
    31561 tctaagaaat acaaagtgca tgatgaaaac aacacagcaa aagttggcga catcgtgaga
    31621 attatggaaa ctcgtccatt atcagctaca aaacgtttcc gtttgttgga agtagtcgaa
    31681 gaagcagtta ttatctaata acacgagatt ttcctatata gattgaatgc aaaatctgaa
    31741 aggaggatac acaacgtgat ccaagcagaa agtcgtttaa aagtcgctga taactcaggc
    31801 gcacgtgaaa ttttgacgat caaagtcctt ggtggatctg gtcgtaaaac tgctaacatc
    31861 ggtgatgtga tcgttgctac ggttaaacaa gcaacgccag gtggggttgt caaaaaaggt
    31921 gacgtcgtta aagccgttat cgttcgaaca aaatcaggag ctcgtcgtac agacggttca
    31981 tatattaaat ttgatgaaaa tgctgcagtg attattcgtg atgataaaag ccctcgtgga
    32041 acacgtatct ttgggccagt tgcacgtgaa ctacgtgaaa acaacttcat gaagatcgtt
    32101 tcactagcac cagaagtttt ataatcttga gacacttatc aaaggaggtg cgaaacagtt
    32161 atgtttgtta aaaaaggcga taaagttaaa gttatcactg gaaaagacaa aaacaaagaa
    32221 ggcgttgtac tagcagcgtt cccgaaaaaa gataaagtta ttgtcgaagg tgtaaatgtc
    32281 gttaaaaaac accaaaaacc ttctcaagct gctcctcaag gtggaattgt tgagatggaa
    32341 gcgccaattc atgtttctaa cgtgatggtg gttgactcta ctggtgtagc tggccgagtt
    32401 ggctacaaag aagtagacgg caaaaaagtc cgtgtttcta aaaaaaccgg tgaagtttta
    32461 gataaataaa agtttaagga aggagggtct tactgaatga accgcctaaa agaaaaatat
    32521 cttaaagaag taactccatc attgatggaa aaatttaact atagctctgt tatgcaaaca
    32581 cctaaagttg aaaaaatcgt catcaacatg ggtgttggtg atgcggtatc aaacgctaaa
    32641 aacttagata aagcagttga agaattaact ttgatcactg gtcaaaaacc aatgattaca
    32701 aaagctaaaa aatcaatcgc tggattccgt ttacgtgaag gaatgccaat tggtgcaaaa
    32761 gttacattgc gcggagaaag aatgtacgaa ttcttagaca aattggtttc tgtttcacta
    32821 ccacgtgtac gtgacttcca tggtgtaagt aaaaaagcct ttgacggtcg tggaaactat
    32881 actttaggta ttaaagaaca attgatcttc ccagaagtag attatgattt agtagataaa
    32941 gtacgtggta tggacatcgt tattgtaaca acagcaaact ctgatgaaga atctcgtgag
    33001 ttgttgacac aattaggcat gccattccaa aaataattaa aggaggcgaa ctacgtggct
    33061 aaaaaatcaa tgattgctaa aaacaaacgt cctgcaaaat tttcaacaca agcttatact
    33121 cgttgcgaac gttgcggacg tccacattca gtttatcgta agttccatct ttgccgtatt
    33181 tgcttccgcg aacttgccta taaaggtcaa attcccggcg tgaagaaagc tagctggtaa
    33241 aaagtaaatt cgcataagaa ggaggtaaaa gatcaatggt catgacagat ccaattgcag
    33301 attttctaac tcgtatccgt aacgcaaaca tggttaaaca tgagtcttta gaagtacctg
    33361 catcaaaaat taaacgtgac atcgctgaaa tcttgaaacg tgaaggtttc gttcgtgatg
    33421 ttgaatacat cgaagatgac aaacaaggcg tgatccgtgt tttcctaaaa tttggtaaaa
    33481 acggcgaacg tgttatcaca aacttaaaac gtatctctaa accaggtttg cgtgcttatg
    33541 tcaaagctga cgaagtacca aaagtattga acggtttagg tatcgcaatc atttcgactt
    33601 ctgaaggtgt tgtcactgat aaagaagctc gtgaaaaaaa tatcggcggc gaagttatcg
    33661 cttacgtatg gtaatcaagt agaatctaca aggaggtgtc tttaagtgag ccgtatcgga
    33721 aataaagtcg ttgttatccc tgaaggagta acagttactc aagacggaaa caacgttaca
    33781 gttaaaggac caaaaggtga attgactcgt actttttcag cagatatcaa aatgaatatc
    33841 gaaggaaacg aagtaacttt cactcgtcca aacgacagta aagaaatgaa aacaattcat
    33901 ggaacaactc gtgcgaactt caacaacatg gttgtaggtg tcagcgaagg tttccaaaaa
    33961 gcgttagaac taatcggggt tgggtaccgt gcacaattac aaggatcaaa acttgtattg
    34021 aacgttggtt actcacatcc agtagaaatc acaccaccag ctggagtaac tgttgaagta
    34081 ccagctaaca cacaagtaat tgttaaaggt gcgaacaaag aagaagttgg cgaattagct
    34141 gcaaacattc gtggaactcg tcctccagaa ccttataaag gtaaaggtat tcgttatgtt
    34201 ggcgaatatg tacgccgtaa agaaggtaaa actggtaaat aatcgtttag aaaaattgca
    34261 tagaagccag cagcaggaaa aaggcatttg gctgaagttt accttcaaaa aaatacattt
    34321 ttacttgtgc cgcagcttct atttttcgaa acgtatttac tgcttgaaat agtagtacaa
    34381 taaaaataaa gaggtgacaa ttgtgattac aaaaccagat aagaacaaaa cacgtcaaaa
    34441 gagacatcgt cgtgtacgta acaatatctc tggtactgct gagcgcccac gcttgaacgt
    34501 ttttcgttct aacaaaaaca tctacgctca aattattgat gacgtagcgg gtgtaacgct
    34561 agcaagtgcc tctaccttgg ataaagaaat ttcaggtgga acaaaaacag aagcagcaca
    34621 agctgtcggt aaactagttg ctgaacgtgc agctgaaaaa ggcattaaag tagtagtctt
    34681 tgaccgtggt ggataccttt accatggccg tgtgcaagct ttagctgaag ctgctcgcga
    34741 aaatggacta gaattttaga agaaggagga ataccattca tggtttatat cgatccaaaa
    34801 catttggaat tagaagaccg cgttgttgca attaaccgtg taacaaaagt tgttaaaggt
    34861 ggacgtcgtc tacgttttgc tgctttagtt gttgtcggtg acaaaaacgg acacgtaggg
    34921 tttggtacag gtaaagctca agaagttcct gaagcaatcc gtaaagcaat tgaagatgcg
    34981 aagaaaaact tagttgaagt acctatggtt ggttcaacaa tcccacacga agtaatcggt
    35041 gcttttagtg gtggtagaat cctaatgaaa cctgctgtag aaggttctgg ggttgctgct
    35101 ggtggaccag ttcgtgccgt attagaatta gctggggtag cagatatcac ttctaaatct
    35161 ttaggctcaa atacaccaat caacgttgtt cgcgcaacag ttgaaggttt gaaacaattg
    35221 aaacgtgcag aagaagttgc agaacttcgc ggcaaatcag ttgaagaatt aatcggataa
    35281 ggaggacaac aataatggct gaattaaaag ttactttaaa acgcagtatt atcggacgtc
    35341 ctcaaaacca acgcgataca gttaaagcgt taggtcttac aaagattaac agttcagtgg
    35401 ttaagccttc taatgaagca attaaaggca tgatcaatac tgtttctcac ctagtggacg
    35461 ttgaagaaat ttaatttaga aaaatcataa ctagaacatt aaggaggtgc caaacctatg
    35521 aaacttcatg aattaaaacc tgctgaaggt tcacgccaag tacgtaaccg tgttggtcgt
    35581 ggtacttcat ctggtaacgg aaaaactgcc ggacgcggac aaaaaggaca aaaagctcgt
    35641 tcaggcggcg gtgtgcgttt aggatttgaa gggggacaaa caccattgtt ccgtcgttta
    35701 ccaaaacgag gattcacaaa cgtgaaccgt aaagactatg cagtcattaa tcttgatgta
    35761 ttgaaccgct ttgaagacgg agctgaagta actcctgttg ctttagttga agctggaatc
    35821 gtgaaaaacg aaaaagctgg aatcaaagta ttagctaatg gggaactaaa caagaaatta
    35881 actgtgaaag cagcaaaatt ctctaaagca gctcaagaag caattgaagc ggctggtgga
    35941 tcaatcgagg tgatctaatg tttagactat taaaagatgc ttttaaagta aaagacatta
    36001 gatcgaaaat cctatttact gtatttgtgt tgtttgtatt tcgcctaggt gcacatatta
    36061 ctgtaccagg cgtaaatgct gcttcattgc aaaacctttc ttctttgcca tttttcaaca
    36121 tgttgaactt ggtaagtggt agtgcgatgc aaaacttttc cattttctca atgggggttt
    36181 caccatacat tactgcttct atcgtcattc agcttcttca aatggacatc gtgcctcgat
    36241 ttgttgaatg gtcgaaacaa ggggaagttg gtcgtaaaaa actgaatcaa gcgactcgat
    36301 atctgacatt agtattggca tttatccaat ctgtcggtat cactgctgga ttcaatcaat
    36361 acgctcaatt aggtttcgtg aataatccta acgtagcaac ttacgtaacg atcggtttga
    36421 ttctaacagc cggtacgatg tttgttactt ggctaggaga acagatcaca gaaaaaggta
    36481 tcggaaatgg cgtatcaatg atcatttttg caggtatcat ttctagattg cctgaaagta
    36541 tcagagaaat ttatgaagat tactttataa atattgatcc ttcagacata tggaaatctg
    36601 ctatttttgt ggccatttta ctagtagcgg tcttgttgat cgtgacattc gtaacatttt
    36661 tccaacaagc tgaacgcaag atcccgatcc aatatacgaa acgcgtagct ggcgcgccta
    36721 caagcagcta tctgccgttg aaagtaaatg cagctggagt tattccagtt atctttgcta
    36781 gttcgtttat tgctacgcca aatgctgttt tacaagcatt gagcggtaga ttcggttctg
    36841 aaaactggta caaagtgatg atggagatct ttaactataa tacagcacct ggtgctacga
    36901 tttatacgat tctgatcgtt gccttcactt tcttctacgc ctttgttcaa gtaaaccctg
    36961 agaaattagc ggaaaacttg caaaagcaag gaagttatat accaagcgta cgccctggta
    37021 aggggactga agagtatgtc tcaagattac tgatgcgttt aagtacagtt ggtgcagtct
    37081 tccttggttt agtggcattg cttccaatca ttgctcaaat ggtttgggat cttccacaat
    37141 caatcggatt aggcggtaca agcttactga tcgttatcgg tgtagcttta gaaactgcga
    37201 aacagttaga aggcttaatg ctgaaacgta aatatgttgg attcattgag taagaaagcg
    37261 tgttgaggtt tcccctcaac acacgttctc acacatttat taggagggaa aacaatgaac
    37321 ctcattttaa tggggctgcc aggcgcaggt aaaggaacac aagctgaaaa aatcattgat
    37381 gcttatggta ttccgcacat ctcaacagga gatatgttcc gcgcagcgat gcaaaacgaa
    37441 acagctcttg gcctggaagc aaagtcttat atggataaag gtgcattagt tcctgatgaa
    37501 gtaacaaatg gaatcgtgaa agagcgacta gctgaaccag atacagagaa aggcttctta
    37561 ttagatggct ttccacgtac gctagatcaa gcaaaagctc tggacgcaat gctaaaagat
    37621 ttgaataaaa aaattgatgc tgtcatcgat atccatgttg gtgaagaaat cttggtcgaa
    37681 cgtctagccg gccgatttat ctgcagcaat tgtggagcaa cttaccataa aattttcaac
    37741 cctacaaaag ttgaagacac atgtgatcgt tgcggtgggc atgaattcta tcaaagagaa
    37801 gatgataaac ctgagacggt taaaaatcgt ctggctatca acatcaaaaa tagtgaacca
    37861 atcttggctt attataaaga acaaggtttg ctaaacacta ttgatggtgc tcgtgaaatc
    37921 gatgctgtat ttgcagatgt acaaaaaatc attgaaaaat agcacgattg cccgtagccg
    37981 acttgcatta tgcagttact cttgtcaagt tcaataaatt atgatagaat atgtcagtcc
    38041 gacttctaga gaatcctcta gatgatccat tttgagaatt ttgaggtggg aactgcagtt
    38101 tccgccattt tatcgtgtga aaatgcgaaa caagtacaag gaggtactgt gcgtggcaaa
    38161 agaagatatg attgaagtcg aaggtacagt cgtcgaaact ttgccgaatg caatgtttaa
    38221 agttgaacta gaaaacggac accaagttct tgctactgtt tcaggtaaaa ttcgtatgca
    38281 ttacatccgt atcttacctg gtgacaaagt aacggttgaa ttgtctccat acgatcttaa
    38341 ccgtggccgt attacttatc gctttaaata attgtactcc gtaaaactac aggaggtatt
    38401 atcatgaaag taagaccatc agtaaaacca atgtgcgaac attgtaaagt aattcgtcgt
    38461 aaaggacgcg ttatggtgat ttgcccagca aatccaaaac ataaacaacg tcaaggataa
    38521 ttggaggtgc tataaatatg gctcgtattg caggagtaga tattcctcgt gataaacgtg
    38581 tagtagtttc acttacttat atctatggta ttggtaacac tactgctaaa aagatcttag
    38641 ctgatgctgg tgtatctgaa gatgttcgcg ttcgtgaatt aacgaatgaa caaacagatg
    38701 ctatccgtgc agaagtagat aaattaaaag ttgaagggga tcttcgtcgt gaagtgaact
    38761 tgaacatcaa acgtttgatg gaaatcggtt cttaccgtgg aatccgtcat cgccgtggat
    38821 tgcctgttcg tggacaaaac acgaaaaaca atgcacgtac tcgtaaaggc ccagctcgcg
    38881 ctatcgctgg caagaaaaaa taattcttaa gtgaaggagg ttaaaacttc atggcagcaa
    38941 aaaaagtgaa tcgtaaacgc cgtgtgaaga agaatataga agcaggtatt gcacatatcc
    39001 attctacatt caataacact atcatcatga tcactgatac tcacggaaat gcattagcgt
    39061 ggtcatcagc tggttcatta ggtttcaaag gaagcaaaaa atcaactcct tttgccgctc
    39121 aaatggcagc agaaactgct acaaaagcag caatggaaca cggattaaag actgttgaag
    39181 taacagttaa aggacctggt tctggacgtg aagcagcaat tcgttcatta caagctacag
    39241 gtttagaagt gactgcaatt cgtgacgtga ctccagttcc tcataatggt gcccgccctc
    39301 caaaacgccg tcgtgtttaa tgagtgccgc tcattttgag ttttatcagt aacgtcattg
    39361 aacaagacac ttttcgtttt gaaaggggta aagataagaa tgattgagtt cgaaaaacca
    39421 agaatcgcaa aaattgatga agaaaaagat tatggcaagt tcatcgttga acctcttgaa
    39481 agagggtatg ggactacttt agggaattcc ctacgtcgta ttttattatc ttctttgcca
    39541 ggtgctgcca tcactaatat tcaaatcgat ggtgtactgc atgaattctc taccatcaaa
    39601 ggtgttagag aagacgtcac tcaaatcatc ttgaacatta aaggcttagc gctgaaaatg
    39661 tatggccaag aagaaaaaac ccttgaaatc gatattaccg gacctgctac agtaactgct
    39721 ggcgatatta tcgttgatag tgatgtagaa attcttaaca aagatatgta catctgtagt
    39781 gtctctgaag gagctacgtt ccatgctcgc ttaacagtga aacctggacg tggttatgtt
    39841 caagcagatg aaaataaaaa agaagatatg ccaattggcg ttcttccagt cgattcaatc
    39901 tacacacctg ttcgtcgtgt gaactatcaa gttgagaaca cacgtgttgg acaccgtgaa
    39961 gattttgata aattgacgat ggagatttgg acagatggtt caattgaacc gttggaagca
    40021 atgagtttgt ctgcgaaaat catgactgaa catttggata tttttgtgaa cttaactgat
    40081 gaagcaaaaa atgctgaaat catggtcgaa aaagaagaaa cacaaaaaga aaaaatgttg
    40141 gaaatgacga tcgaagagtt agacttgtct gttcgctctt acaattgttt gaaacgtgca
    40201 ggaatcaata ctgtacaaga acttacaaat aaatctgagc ctgaaatgat caaagtccgt
    40261 aacttaggac gtaaatcatt agaagaagtt aaagcaaaat tgcacgactt aggtttaggt
    40321 ttacgtaaag aagattaaaa ccttttacga aggagggaaa ccaacgtgag ttatcgtaaa
    40381 ctaggacgca catctagcca acgtaaagcg atgttgcgtg atttaacaac tgatttatta
    40441 atcaacgaac gtattgtgac tactgaagct cgtgcgaaag aagttcgctc aactgcagaa
    40501 aaaatgatca ctttaggtaa acgcggagat ttgcatgcac gtcgtcaagc tgcggctttc
    40561 gtacgtaatg aagttgccag cgtgcgtgaa gaaaatgagg acatcgtcat tgaatcagct
    40621 ttacaaaaac tgttcaacga tattgcacct cgttacgcag aacgtcaagg cggatacaca
    40681 cgcattttga agacagaacc aagacgcggt gatggtgcac caatggttgt cttagaactt
    40741 gtctaaaatc acactaataa catcacttta tagatgattc ttttagagtg ttatgatgtt
    40801 ggaagatatt tttccgagtc tagctcagcg ctgctcctca agcgaatatc gcttggggag
    40861 taagcactca tcataaagtg tttttttata ccttcaatta cctgtcatga cagatatgtt
    40921 tgaaggcata aaaaaacatt ttaagcgaga aataattgaa aattttgtct ttttttgata
    40981 gtgttagtag gaactttagg atgaagaagg agtaatcatg gagccaatta ttgaattaga
    41041 aaagataaat tataaatacc agccagatga tcttcgcccc gcattgaaag atgtctcctt
    41101 tacaattgat aaaggtgagt ggatcgctat tattggacac aatggttcag gaaaatcgac
    41161 acttgccaaa acgatcaatg gattgcttct gccagaatca gggatcgtga aagtgggaaa
    41221 tcaaatattg gatgaagaaa atatttggac gatccgacag atggttggaa tgatttttca
    41281 aaatccagac aatcagtttg ttggttcgac agtagaagat gacgttgctt ttggtcttga
    41341 aaatcaaggg attcccagag aagaaatgct agtgcgtgtg aaagatgcac ttgagaaagt
    41401 tagaatggct gagtttgctt cacgcgagcc tgctcgttta tcaggagggc aaaaacaacg
    41461 tgttgccatt gccggcgtgg ttgccttacg acctgatatc attattttag atgaagcaac
    41521 cagtatgcta gatccagaag gtcgtgaaga agtgatctct acgatcaaga agatcaaaga
    41581 agaaagtcaa ttgaccgtta tatctatcac tcatgatatt gatgaagcag ctaatgccaa
    41641 tcggatacta gtgatgagac aaggcgaact tgtccgtgaa ggtacaccaa aagaaatttt
    41701 ttctgctggt cctgaactga tcgacttagg tctagatcta ccatttcctg aaaaactgaa
    41761 gagcgcactg aaagaacgtg gggttgatgt gcccagtgaa tatatgacag aagaaaggat
    41821 ggtggattgg ttatggacat ccgttttgaa caagtagact tcacttatca gccgaataca
    41881 ccatttgaac aaagagcctt atttgacatc aatatgacga tcaaagaaaa cagttatact
    41941 gcattagtag ggcataccgg aagcgggaaa tcaaccttac ttcagcattt gaatgcgctt
    42001 gttaagccaa caagcggtac agtccatatt ggagaacgag atattcagcc ggatacggat
    42061 aataagaatt taaagcccat ccgaaaaaaa gttggcatcg tctttcagtt tccagaagca
    42121 cagctattcg aagaaacggt agcaaaagac attgctttcg gtcctaaaaa ctttggtgtc
    42181 agcgaagaag aagcactagt cctagcaaaa gaaacattag aacaagttgg gctggatgaa
    42241 agctatttgg aacgttcgcc atttgaactt tcaggagggc aaatgcgtag agtagcaatc
    42301 gctggtgtgc ttgccatgag gccggaagta cttgtattgg atgaaccaac ggcaggactt
    42361 gatccccagg gcagaaaaga aatgatggag atgttttggc gtcttcataa ggaacagcag
    42421 atcactattg tattagttac gcatttaatg gatgatgtag ccaattttgc agattatgtc
    42481 tatgtgttgg aaaagggacg aatcgttaat agcggggagc cgcaagaggt ctttcaaaac
    42541 attgaatggc taaaagaaaa acaactaggc gtacctactg ccaccgagtt tgcagaagaa
    42601 ttaatggcta aagggatgaa ctttgctacc ttgcctttga cagctgaaga attagcagac
    42661 gctatcgtcc attatgctgg aggaaatgcc catgatgaat aaattgatat ttggtcgtta
    42721 tatgcctgga gattccttga tccatcgatt ggatccccga gctaaattat tagccagttt
    42781 ttattttatc ggaatcattt ttttagcaaa taattgggaa acgtaccttt tgttggcatt
    42841 tttcacatta ctttctgtct tcttgtcaaa agtagatttg aaatttttta tccgaggcat
    42901 tcgcccattg atctggctaa tcctcttcac tgtagctttg caggtcttgt ttacaagtgg
    42961 cggagaaact tattggagtt ggggtgtttt ccatatcact gaatttggtg tgatgaacgg
    43021 cctctttatt ttctgtcgat tcgtgttgat catttttatg tcgactctac tcacgcttac
    43081 tacaccgcct ttggaattat cggatgcgat tgagtacttg cttcgtccat tgaaagccgt
    43141 acggttccca gtccacgaaa tttcattgat gctgtcgatt gctcttcgtt ttgtgcctac
    43201 attgatggac gaaacagaaa agatcatgaa cgctcagcgc gctcgcggtg ttgattttgg
    43261 tgaaggaaac ctagttcaga aaatgaaagc agtcattcct ttgctgattc ctttatttgt
    43321 cagcagcttc aaccgtgcgg aagatcttgc gactgctatg gaagcaagag gctacaaagg
    43381 tggggaagga agaacgaaat atcgtgtatt gcattggcat aatagagaca cgatggttat
    43441 cattgctttt gtattattaa ctgtgctact gatcttttta agaggataaa cgttcagtgg
    43501 gccgctatct attcttttct tgctgatctg tttgcaagaa aggatgcaag cagtgtatga
    43561 caagaaatat tagggtctgt ggcagactaa ggtcacaggc ctctttcttt tatctgaaaa
    43621 gaaagaacga aaaaatttta tagaaaaatc attattccag ataacctttt aggaacaaag
    43681 gagagaaaaa catggtacgt tacaaagcaa tcattgcgta cgacggaaca aattttaatg
    43741 gattccaaaa acagccaaat gggcgtactg ttcaagaaga agtagaaaaa acattacaaa
    43801 aaatggcaaa tggaaaagaa atcacggtct ttggttcggg aaggacagat gccggagtac
    43861 atgcgatcgg gcaagtaatc cattttgatt atccagagga gcgtccgtta gaacgaatgc
    43921 gtttcgcgct agatacccag tcaccagagg atattgctgt aagacaagta gaaattgttt
    43981 cagaagactt ccacgcaaga tatttggtca aagaaaagac gtatcaattt cgagtggata
    44041 tagggaaacc acgcagtcct tttcgacgcc attatgcaag ttattttcct tacccacttg
    44101 atttggaaaa aattcagcgg gcgcttcctg atttattagg gactcacgat ttcacctctt
    44161 tttgtgcatc aggaagctcg atagaggata aaatacgtac gatctacgag gctaagatgg
    44221 aggtaaatga aacaaaagac gaattgttgt ttacatttag gggaaatggc ttcttgtata
    44281 agatgatacg gatcttagta ggaacgcttt tgaaaattgg caatggccga ttgcctgaag
    44341 atagtatccc agatattatt gcgaaaaaag atcgtaatgc agcaggacct acagcacacc
    44401 ctgaaggact ttatttgtat gaagtgaaat atgaataaga ggttatgata gaagtggtca
    44461 gcttcaagaa ataagaaggt atttacgaaa attacttctc aaaattttgc aaaatttcgg
    44521 cttattttcg aagaagctac ttctggaaca ccgtttaaga aagaggtcgt gagcctgtca
    44581 tttaactctg agtcacaaag agcaatttca aaaaacagct tctcatcttt gatgaatatc
    44641 gtgacttgtg gacgaggcgt tgataagtga atcatctttt tgttacctat tttttctgta
    44701 accaaaaaac gatggaacgt tgttttgatt gcccttggtg cggacagtct gaacaggttc
    44761 catcgtttct ttattttcta cgtcttcttc tgtgtgtgtt tcgtggttgt tcttctattg
    44821 ctggatcaat cgtgccataa aaggggcgat tgcggtcaag gtaccagaca taaagaataa
    44881 acaatgcaat acataggaca aaaaggattg cacctatcgt aaatccttca ggcaagtaac
    44941 tgaaagtgac agtatgttta cctgctggaa cttttatcat caagaatgcg tctttgaaat
    45001 tgctgacctt gactggtttc ccatcaattt tagctttcca tcctttgtca taaggaatag
    45061 ttgtcaccaa catggaatct tcagcggtat ttacagtccc agaagctttt ctgccagtgg
    45121 cttccatttc gacacctttt tctttgatct gattcattgc ggcttgatag gctacaacat
    45181 ctagaccaac gactttaggc tccatgaaac ttatttcttt cgtcccataa aaacttgcag
    45241 taaatgtcac tgttgtctct tgaggataat agccaagatc atagtattga ccagaaatgt
    45301 tgatttgcgt ttttcgcgat gtaccattga cagtgatcgt cgcactagaa ctttctaatt
    45361 gcgcaaaatt ggttgggaac aaacttaagt aagcttgtgt atttgcaggc acatggactt
    45421 cccaagtgag ctcttttggc tggttatctg ctgtttctga gtaagtgacg ccagtcgttg
    45481 tctttgtgat cgtcgtattc aactgattct tcaaagtagg tgtatagaat gtaaaatact
    45541 gttcatttag accagacaat gtattgatca agtttgtctg actggtcaaa ttatctgtca
    45601 ctggctgctc aacatcatta acggacaacg gtgcagtaaa tgctagtggc aatgcattga
    45661 cattctgata cagtgtatag tcgccgcttt gaccagcttg gcggaagcca tatttactga
    45721 acgtgctttc gtttttagaa atgttatatt tgattcctgt aaacccatcc atcagcaaag
    45781 tattattgtt ataacggata ttcaaatttg tccctcttga acggaaacct aatttatcca
    45841 ggtaagagga agaattacga tttcttatag aagaaaacaa actgatacca ctgtaaccat
    45901 agttgatcga atcgtttgca gatactggat ccagattttc caagcggtaa aacgagtcgg
    45961 aatctttttt tgtttcattt acaagagctt tcaagtcagg ataaggatca gtatataaac
    46021 tgcgggaagc ataattccaa tcatcaagaa tgccatggat catactgttc gtattgacca
    46081 gtgcttcagc agacatgatc aataataaca atatactcag atgattgaat gaaagttttt
    46141 tatgctgata aaatcccaca gcaaaagcat aaatccctaa gaaaaccact gtcaatacaa
    46201 aggaagtcat cgtcacatag gtgtagcttc cttcaggttt gatgccccat gccaccgcaa
    46261 agagagcagc tacgaccaaa atgattccta acaacagccc accatcgtct tgttctatct
    46321 tttcccatcc ttttcctgca agcaggagca ctaagaaaga gaacagatag gcataacgga
    46381 ataaaaacat attcggtgca tgcataccat gccagaacaa attcaatgga acaagataaa
    46441 aacttgcgat aagtatggcg aaaagactgc cgtacaaaaa tttatttttc aacggtactt
    46501 tccgactggc aaaataaaac agacagagga ctaatggtag cagacctgcg taaacgaacg
    46561 gaatcgatcc gtattttgtc gtgtcataaa caccaatcat atttttcatg attagatcca
    46621 gataagaagt cgcttctgtt ttcaacgtag tgatctgact cagtgcctcg ccatttgacc
    46681 gaagatccag cactgctggc aatacaatga tcattgaagc gccgcctgcc agtaaagaag
    46741 tgataccata aggaacaatc gtttttttat attctgacca gtttgtcaat agacgggcga
    46801 taaaatataa gaaagaaaat acgccaatca taaaacccat ataaaaactg gataaaaaca
    46861 agagtaaata actgataaat aaaagtttcg gccgtttaag atccataaca cgatgaatcc
    46921 ccaaaatcac taatggaaga taaacaaatg catccagcca catgatgatc tccgaatgtg
    46981 cagtgataaa tgagatcaat gcgtaactga cgctaagtgc aacatgcccc cagcgaggaa
    47041 tcttgaacgt acgtgcaaga aaccaaaaag ccaatccggc acagccgacc ttaagcaaag
    47101 tcagaacata cagtgcatct ggcatcatct ggtttggaaa aaagaacacc agtggcgtga
    47161 agatcccgcc tagatagtaa gaaattaaag ataaataatt caatcctaga gaagcattcc
    47221 atgtataaaa aatactttgc tttccaagaa gaacatttcg gaagctggca tgaaaattag
    47281 aaaactgtga aaaggcatca cttgccaaaa tacttctgct gcttccagga tagattccga
    47341 tacttagata aacaagtgct aaaatcaaaa acggaatcag gaaactagct gctgcgtagc
    47401 gtccgttttc tctaaagaat gactttaaat tttttttcat atttattttc caccaaattt
    47461 ctatgaagtt gtattcagtt tatcatatcc ttg
//
LOCUS       DARBEC010000011        45524 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.011, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000011 DARBEC010000000
VERSION     DARBEC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.011"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCL80_000593"
     CDS             complement(26..1522)
                     /gene="lysS"
                     /locus_tag="SCL80_000593"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEG1106485.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCL80_000594"
     CDS             complement(1633..2637)
                     /gene="dusB"
                     /locus_tag="SCL80_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEG1106486.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCL80_000595"
     CDS             complement(2638..3525)
                     /gene="hslO"
                     /locus_tag="SCL80_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEG1106487.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCL80_000596"
     CDS             complement(3742..5853)
                     /gene="ftsH"
                     /locus_tag="SCL80_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEG1106488.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCL80_000597"
     CDS             complement(5943..6488)
                     /gene="hpt"
                     /locus_tag="SCL80_000597"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEG1106489.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCL80_000598"
     CDS             complement(6488..7933)
                     /gene="tilS"
                     /locus_tag="SCL80_000598"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEG1106490.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8053..8523)
                     /locus_tag="SCL80_000599"
     CDS             complement(8053..8523)
                     /locus_tag="SCL80_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEG1106491.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(8569..8994)
                     /locus_tag="SCL80_000600"
     CDS             complement(8569..8994)
                     /locus_tag="SCL80_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEG1106492.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9061..9330)
                     /locus_tag="SCL80_000601"
     CDS             complement(9061..9330)
                     /locus_tag="SCL80_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEG1106493.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9341..10936)
                     /locus_tag="SCL80_000602"
     CDS             complement(9341..10936)
                     /locus_tag="SCL80_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1106494.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCL80_000603"
     CDS             complement(10951..14472)
                     /gene="mfd"
                     /locus_tag="SCL80_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEG1106495.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14489..15049)
                     /locus_tag="SCL80_000604"
     CDS             complement(14489..15049)
                     /locus_tag="SCL80_000604"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEG1106496.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15402..16376
                     /locus_tag="SCL80_000605"
     CDS             15402..16376
                     /locus_tag="SCL80_000605"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1106497.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            16546..17946
                     /locus_tag="SCL80_000606"
     CDS             16546..17946
                     /locus_tag="SCL80_000606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEG1106498.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18024..18746)
                     /locus_tag="SCL80_000607"
     CDS             complement(18024..18746)
                     /locus_tag="SCL80_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1106499.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(18825..19331)
                     /locus_tag="SCL80_000608"
     CDS             complement(18825..19331)
                     /locus_tag="SCL80_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1106500.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19454..21253
                     /locus_tag="SCL80_000609"
     CDS             19454..21253
                     /locus_tag="SCL80_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEG1106501.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21321..22895)
                     /locus_tag="SCL80_000610"
     CDS             complement(21321..22895)
                     /locus_tag="SCL80_000610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEG1106502.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCL80_000611"
     CDS             complement(23150..23659)
                     /gene="mreD"
                     /locus_tag="SCL80_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEG1106503.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCL80_000612"
     CDS             complement(23663..24517)
                     /gene="mreC"
                     /locus_tag="SCL80_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEG1106504.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(24672..25310)
                     /locus_tag="SCL80_000613"
     CDS             complement(24672..25310)
                     /locus_tag="SCL80_000613"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEG1106505.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25311..25961)
                     /locus_tag="SCL80_000614"
     CDS             complement(25311..25961)
                     /locus_tag="SCL80_000614"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEG1106506.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCL80_000615"
     CDS             complement(25974..26873)
                     /gene="rsgA"
                     /locus_tag="SCL80_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEG1106507.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCL80_000616"
     CDS             complement(27038..29107)
                     /gene="pknB"
                     /locus_tag="SCL80_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEG1106508.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29104..29844)
                     /locus_tag="SCL80_000617"
     CDS             complement(29104..29844)
                     /locus_tag="SCL80_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEG1106509.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCL80_000618"
     CDS             complement(29857..31215)
                     /gene="rsmB"
                     /locus_tag="SCL80_000618"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEG1106510.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31215..32153)
                     /locus_tag="SCL80_000619"
     CDS             complement(31215..32153)
                     /locus_tag="SCL80_000619"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEG1106511.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCL80_000620"
     CDS             complement(32146..32637)
                     /gene="def"
                     /locus_tag="SCL80_000620"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1106512.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCL80_000621"
     CDS             complement(32652..35060)
                     /gene="priA"
                     /locus_tag="SCL80_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEG1106513.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35313..35618)
                     /locus_tag="SCL80_000622"
     CDS             complement(35313..35618)
                     /locus_tag="SCL80_000622"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1106514.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCL80_000623"
     CDS             complement(35623..36237)
                     /gene="gmk"
                     /locus_tag="SCL80_000623"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEG1106515.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(36724..37008)
                     /locus_tag="SCL80_000624"
     CDS             complement(36724..37008)
                     /locus_tag="SCL80_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106516.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37026..37916)
                     /locus_tag="SCL80_000625"
     CDS             complement(37026..37916)
                     /locus_tag="SCL80_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEG1106517.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(37936..38154)
                     /locus_tag="SCL80_000626"
     CDS             complement(37936..38154)
                     /locus_tag="SCL80_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEG1106518.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38124..39824)
                     /locus_tag="SCL80_000627"
     CDS             complement(38124..39824)
                     /locus_tag="SCL80_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEG1106519.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40091..40864
                     /locus_tag="SCL80_000628"
     CDS             40091..40864
                     /locus_tag="SCL80_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106520.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCL80_000629"
     CDS             complement(40945..41514)
                     /gene="thiT"
                     /locus_tag="SCL80_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEG1106521.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(41580..41682)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(41688..42659)
                     /locus_tag="SCL80_000630"
     CDS             complement(41688..42659)
                     /locus_tag="SCL80_000630"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1106522.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCL80_000631"
     CDS             complement(42753..44126)
                     /gene="glmU"
                     /locus_tag="SCL80_000631"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEG1106523.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCL80_000632"
     CDS             complement(44629..45447)
                     /gene="purR"
                     /locus_tag="SCL80_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEG1106524.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
BASE COUNT        12400 a         9876 c         8237 g        15011 t
ORIGIN      
        1 aattccttag acagctttta ttcgattatc tcattgttgg gaatagcaaa acatcccgaa
       61 ttgattgtgc atcagttagc aacattacta atctgtcgat cccgatgcct agaccaccag
      121 ttggcggcat accgtattct aaggcttcca agaagtcttc gtctacaccg tgagcttcgt
      181 catttcctag ttcacgttct ttttcttgcg cttcgaatct ttctctttga tcaattggat
      241 cgttcagttc tgtaaaggca ttagcaaatt cgcgccccac tatgaatagt tcgaatcgat
      301 ctgtgaatcg aggatcttct ggatttttct tcgctaaagg cgaaacttcg actggatgtc
      361 cgtatacaaa cgtcggttgt gtcaatgttt cttcaacaaa tgtttcaaag aattcattga
      421 taacatgtcc gacacccata gcttcggtta cttctacatt gtgtttttcg gctaaagcta
      481 atgcttcttc caatgtcatt tcttgccaga agtctacacc agtttgttct ttgattgcat
      541 ctaccatatg gatgcgttta aagtcacttt ctaagtcgac agcttgtcca tcatatgtga
      601 tttttgctgt tcctagtact ttttcagctg cgttacggat aataccttct gtcagattca
      661 ttacatcatt gaaatcagta tatgctgtgt atgcttctaa catcgtaaat tctggattat
      721 gtgtagtatc aattccttca ttacggaaaa cgcggccgat ttcataaact ttttccattc
      781 cgccaacaat tagtcgtttt aaatgaagtt ctagcgcaat acgcaaatat aaatcaatgt
      841 ccagtgcatt atgatgcgtg ataaatgggc gtgcggcagc tccacctgcc tcattgtgca
      901 atactggtgt ttctacttca atatagccat tgccatctaa atagcgacgg atttcgctga
      961 taatttggct tcgtttcata aatcggtcaa agctttcgcg attactgatt aaatccaagt
     1021 agcgttgtct gtagcgttgt tcaatattcg ttaagccatg gtatttatct ggcagtggac
     1081 gaagtgcttt tgacaaaatc gtgatttctg aagctttgat agaaacttcg cctgtgtctg
     1141 ttttcatgat ttgtccagtt acaccaaaga aatctccaag gtctgcatgt ttgaatactt
     1201 cataggcctc atcacctact tggtcttttc ttacataaat ttgaatttgg ccttcacgat
     1261 cttgtagatg cgcaaatcca gctttccctt ttcctcggcg agtcatcata cgaccagcta
     1321 cactagcggt caatcccatt tctgctagtt cttcttttgt tcttggatca aatagttcat
     1381 gcaattcttc tgagttgtgc gtgcgttcaa atcgttttcc aaatggatcg attccatctt
     1441 cacgcaattg ttccattttt tgacgacgta caagcatttg atcatttaaa tcttctactt
     1501 gatgttgttg ttcctgtgtc acaaaagttc ctccattctc atgttaatct ctttcctata
     1561 atgccaatga aacacaaaaa aagcaagccg aacttggaat ttcggcttga aaattcacga
     1621 aaaacttaga aatcacaacg tttgtttcaa tagtactttt tccgcttttt ctacaaattg
     1681 atccagcagg tcattgattt cttgctgttt ttctgtctga ttgattgcta ctttcacttt
     1741 cgctgctcga ggaatccctt ttaaataata agccgcatgt gttctgaatt ctctacaagc
     1801 tactttttct cctttgagat ctgctaagcg ttgtaaatga agtttagctg tagcaatttt
     1861 ttcggctggt gttggttcag gaatcagttc ccctgtttcg agataatgct gtgtgcggtg
     1921 aatcatccat ggattgccca gcgctgcacg accaatcatc acaccgtctg caccgacata
     1981 gtcaagcatc cattttgcat cttctggcgt tttgacatcc ccgttcccca taaatggaat
     2041 cgtcaaatgt tttttcactt ctttcaatac ttcccagtcc gcttttcctt catacatctg
     2101 aacccgtgta cgtccatgca tagcgatggc acttgctcca gctgattgtg ctgctaatgc
     2161 attttctaca gcaaagacgt gctcttcatc ccaaccaata cgcatcttga ttgttactgg
     2221 gatatttact gctgaagaaa cagcttggac catttcgtat actttctcag gatctaaaag
     2281 ccattttgct cctgcttctg ctttgattac tttatttact ggacagccca tattgatatc
     2341 tattatatcg gcagctgtgt tttcttcgac aaattgagca gcttcaacta gactatcttt
     2401 atttccgcca aatatctgta aacttaatgg atgttctgtt tcttcaatgt ggagcatctc
     2461 taaagttttt ttgtttctga aatgaatacc ttggtcactg atcatttcac atacgactaa
     2521 gcccgcacca aattctttga cagtgactcg aaatgctgaa tttgttattc cagccatcgg
     2581 tgcaacaacg acacgattag ggatttccac attgccaatt ttccatgtgt gttccactta
     2641 aatccctcct aaggcttctt tcttcaatcc atctaattct tcttcgctat acttgtattt
     2701 gttattacag aaagaacaaa ccgcttctgc accgtgatct tcgtctatca ttgcttggat
     2761 ttcttctgca cctaacgcga taattgcttc tgcaaatttt tcttttgaac agtcacattt
     2821 gaattgaaca ggcatttttt ccaaaatgtt caattcttct gtaccaagca atcctttcaa
     2881 tatttcttct ggtgtctgtc cttcatctaa gagggtagag attcttggtg tcgtttttaa
     2941 ttgttcttcg atcttgctga ttgtttcttc atcggcacct ggcatgattt ggatcatgaa
     3001 tcctcccgcc gtcttgatgc tgtcgtcagt attgaccaat acactcagtc caacagctga
     3061 agggatttgt tctgagatag caagatagta agtaaaatct tcagcgattt ctccagaaac
     3121 aatcggcgtt tgcccagaaa aaggttcttt taatcctaag tcttttgtga cagtaaacat
     3181 tccttgtgtt ccaactgctc cccgcacatc gattttacct tcactattta gaggaagact
     3241 gagatgcgga tttttgatgt aacctttcac atctcccttt ccatttgcat ctactacaat
     3301 tcctgttgct ggaccatcgc cttggattcg aacagttagt ttgtcttctc ctttgagcgt
     3361 cgcaccaagt agcagactac cgaccaatgt ccgtcccaaa gcagctgagg aggtgtgcca
     3421 agtatcatga cgtctttgtg cctccgctac ggtatttgtt gcttgaacgg cgtatgctcg
     3481 gacgaatccg tcgtgagcaa gtgccttaac caaataatct ttcatttgtt atgtactcct
     3541 ttttccgttt ttaaactcat agtgaatcat actagcaact actgcttttt tcaactttgg
     3601 aatgcttctt ttaaaaataa ttcagaatca tctagaaaac aggacatcac cctatttaca
     3661 acttaaataa agaaatctcc cgaaaaccgg tttttgttcc ggttttcggg agtcatatca
     3721 gatcaattga cctatatttt tttatttgta ttgatcgtct gaatgattat cttctgattg
     3781 ttgagaatct gaatcatgtt ctgctttatt ttcctcattc agttcatgtt ttgcttcttc
     3841 aaaatcacgt ttttcttccg cttgtttttc agcgtctttt tcttccaaag cacgttttgc
     3901 ttcttcaaaa gttttcgctt ctttttcgct tggataatca tttgactcag taccttcagg
     3961 catcacaccg tgttcaaaca atgacttgat tgctttggca tccaatgttt cgaattccaa
     4021 aagtttttca gcaatcagtt tatgctgtgc acgatgagct tcgatgattt ctcgagcttt
     4081 atcatgagct tccatcaaga tttttcgtac ttcttgatca atctcgaaag cgacttgttc
     4141 cgagtaagct tttgtttgac catagtcgcg gccaacaaac acttgatggt ttccttcata
     4201 ttgaacagga cctaaacgat cactcattcc atattctgtt accatgcttc gtgctagagc
     4261 tgtcgcttgc tcaaagtcat tggaagcacc agttgattgg acattgaaga tgatttcttc
     4321 agctgtacgt ccaccaagta agcctacgat ttgttcaaac atatcttctt tcgtcatcaa
     4381 gaactgatct tcttttggta aagcgatcat atatccgccg gcgcgtccgc gagggatgat
     4441 cgttactttg tggacgaccc gagcgcgact caatactaat ccgacaatcg tatgaccagc
     4501 ttcgtgataa gcaaccattt cgcgttcttt tttgctgatt acgcgatctt ttttagctgg
     4561 acctgcaatc acgcgatctt ccgcttcatc gatatcagaa gcatcgattt tctttttatt
     4621 tcttctagca gctactaacg ctgcttcatt caagacattt tctaaatctg ctccggcaaa
     4681 acctggtgtc tgttgagcca caactttcaa gtcaacgtca tctgccatcg gtttattccg
     4741 tgcatggact cgcaagattg cttcacgacc ttttacatct ggacgtccaa ctaaaatctg
     4801 gcggtcaaaa cggcctggac gtagtaacgc tgggtctagt acatcagaac ggttggttgc
     4861 agcaatgacg attactcctt catttccgtc gaagccatcc atttctacaa gcaattggtt
     4921 caatgtttgt tcgcgttcat catgtccgcc gcccataccg gcaccacgtt gacgacctac
     4981 cgcatcaatt tcatcaatga aaatgatagc tggtgcgttt ttcttcgctg tttcaaacaa
     5041 atcacgaaca cgacttgctc caacaccgac aaacatttct acgaaatcag aacccgagat
     5101 cgagtaaaac ggtactccag cttctccggc aacagctttt gctaataagg ttttacctgt
     5161 tcctggaggt ccttctaaaa gtacacctgc cgggatacgg gcacctaatt caacaaatcg
     5221 gcgaggatct ttcagaaatt caacaacttc aactagttct tgtttttctt cttccgcacc
     5281 cgctacatca gaaaaacgta cgcgattagc ttttttgtct gcttctttgg cttttgattt
     5341 accaaagttc atgacacggc cgccaccgcc accgccgccg ccttgttgtc ccatcatcat
     5401 gtaaaagaaa aagatgatga ttacgattgg caagaagcta agcaataggg aaacccatat
     5461 accgctgctt gattcttctt ttactgttgt agcaactttg ttttcttttg ccagttctgt
     5521 tacttggctc aatgttgtat catttggcaa aatgattgtt gtgaaatggg tagtagtcgt
     5581 atctgttgaa cctaagatag ctaacccacc gctgttagcg acggtctgct gttctttgta
     5641 ttcaccagtg atcttatata caccatttgc aggctgcact tcaaattctt tgatcttgcc
     5701 tgcttgtagt tgttctgtga attttgagta ctcgatatct gacgatcgct ggccgttatt
     5761 accaaaaatg aaataaacga ccattaccat cgctaagatt acgaggacat aatacaaggc
     5821 atttttcatc atgccgttgt tctttttgtt catagttgcc ctccctaagc gtcaatttaa
     5881 tttaacagtt ttttatcaat cctgaccatt ctattgtacc ataatttaat tgagatacag
     5941 cattaatttg attgataaac actaggtttc aatacaccga tgtacggaag gttacgatat
     6001 gcttctgcat aatccaagcc ataaccgacc acaaattcat ttggtacgtt gaagccaaca
     6061 taatctgctt cgatatttac tactcttcct tcaggtttgt caagtagtgt aacaattttg
     6121 actgattttg ctttgcggta tttgaaaaga tccactaaat aagcaagtgt tcttccgctg
     6181 tcaatgatat cttcaacgat caacagatcg cgaccttcta cattcgtatc taagtctttg
     6241 acgattttta cttcccctga cgagactgtt gcattcccgt agcttgaaac atccatgaaa
     6301 tccaattcca aatacgtatc gatagaacga acgatatctg ccatgaatgg taccgctcct
     6361 tttaagacac ctactactag gggattggtg ttttggtaaa tttctgttaa ctctttgcct
     6421 agttctttgc aacgaacttg tatctcttct tgtgagatca aaattctctc aatatccttt
     6481 tccaacatca ggttctcctt ccaattgctt ctttttgata tttgtaaaga agtatgtagt
     6541 gtattttatc agtttcttca cgaatactca aatatgaaaa ggcaaaaggt atcaagctat
     6601 atatatttct tttggcatct gtaatgatcc aagcatgctg cctttgtgaa ttaggtactt
     6661 tctgatcgat aaaatatcta gatacttttt ttgtgagatt ctctgttaat ttaatgcggt
     6721 ctcctgcttt tcttttttcg acatacagtt gcacatcagc aggaatccaa atgtcatgag
     6781 aaaatagaga ccatgaatct gttgctttga tgtcccctgc attttcaggt ttgaccagac
     6841 caatccattc tgtttcattc aaatatattc cttgttctgg aatcagcaac aagcgctggt
     6901 cttctgtctg gctagttgct tgtttttctt ttttagagag tacaacaaaa tgatactctc
     6961 ttttcagtat ccaattattg ccaatactga gttgccattg gctttttttc tgttgccact
     7021 ggtctaaaat cgactccatt tgttgttctt tcaaaataat tcctgttttt gagaatgctt
     7081 tgtcgaaaaa ggagtaaaaa aaatagtatc gttcagcatc tgtcatttct tcaatcattt
     7141 ctgcagaaaa tcgaaattgt tcctcccact gttcgacttc cttttcgatc aactgtccca
     7201 tatatttttg aatgacttgg tctgcccatt ggatctgttg cgaaaattgc tggaaatgtc
     7261 tcaacgcctg tgtattttct tgtttcaatt gaggaactac caaatgacga agccgatttc
     7321 tctggatatc taacaattgg tttgtctgat cctcaaagta aacaagctgc gattccgctg
     7381 cataagtata taggtcttct ttgctaaaac tcagcaacgg acgaatcagt ctgcccgtag
     7441 caaatggttg tacttctttg attccagaaa aagtatttaa ctgtccccca cgaacgattt
     7501 tcattaacac cgtctcgatt tgatcatctc cgtgatgagc agtcatcaac gtgtcatatt
     7561 gtccttcttc catcacgcga gtgaattctt gataacgaaa gttacgagct tctgtttcta
     7621 tggcggattt ggctggatct tcccaaatac ttacatataa agggagattc ttctcttcac
     7681 aatatgagcg aagataggca gcttcttctg ctgattcttc tcttaattgg tgattgatgt
     7741 ggacgatacc taaagaaaaa ccgatttggc gttgcacttg taaaagtaaa tggagcaaaa
     7801 ccatcgaatc aacaccacct gataccgctg ctaaaattct cgatccttgt tgccagaatc
     7861 cttgttcatt cccgtgccga ataaatgact gctgtaatag cggtgctttt ttcccgcctt
     7921 tttcaacgtt catcctttcc acaccctttc ttcctaaaaa aaagagagcg ggaaaacgtc
     7981 taaaacgaca attaacctgc cctctgttga tactatgacc caaatagtcg cactatcatt
     8041 atttgcggct ttttagctgc gacgtccgcc acggccaccg cgctttcctt ctgtgtttcg
     8101 cttcaatgaa gataagcggt catcgctatc tttcaaaaat gaactcatca atgaatcgaa
     8161 gtcttctttt tgagctgaag gttgactgcg agaaaaagat tttttctgtg ctggacgatt
     8221 tcggttgtca cggggatctt tttgttgata ttttttagga cggctttctt ctccagattc
     8281 agcaggttgt tcctgcgctt tccggataga tagtccgatt tttccatcat catttactga
     8341 aataacttta actgttactt catctcctac acttaaaaca tcatggatgt cttttacata
     8401 gccatttgac acttcgctaa tatgaactag acccgttttt ccagcaccta gatcaataaa
     8461 cgcaccaaaa tttgtgattc ctgatacttt tccttgcagt tttgctccta cctcgattga
     8521 cataaaaaaa atgttcctcc taattatttc ccttttttat attcctgctt attttgcagt
     8581 tgtagaacta gttgattgat ctgtttcatt tgtcgagctg tctgtatttg attctaatat
     8641 aggataaact ttttcaccgt cttttgagta aaagaatttg cttctggcaa gtttagctac
     8701 atactcatca tctttcagca atgaaacatc ttggtgcagc tgatcaacgt ctgcgttgac
     8761 ttctttcatt tcagtatttg cttcgactct caattcgttc aattgatgca tcctttgcat
     8821 gtcatggaaa atctgcacac ctattatcgc gaaaatgacc aaagcaccaa caaaaattgc
     8881 agccagtctg cgtcttcgaa aaatcagttg acgttgttgt tgctgaaatt cagcatactt
     8941 tttctttgca tattccgtat ctagcgcagc gattttattg ctttttttcg tcacttggat
     9001 tcgctccaat ctttttgatt gtctttttca ttataccaat atcgtgaaag cttgtctaat
     9061 ctatttctct agtgtttctg tgcgattttc agaaataatt tcgtacattt tttgtgcatc
     9121 ttcttttttg gttgattcat gtaattccgt aattttcact tccaacgttt tgttgccaaa
     9181 ctggactttc aaaatatcgc ctactttgac atcactggaa gatttcgcca atttaccgtt
     9241 gatttgaatt cttcctttat cagctacttc ttttgctact gatcgtcttt tgatgattcg
     9301 ggaaattttt aaaaatttat ctaatcgcat atcttattcc tcacttttta atttttttat
     9361 ttaatcgcag gattttctta ccaaaaggca acattagcca ttctcgtact gtaaacagac
     9421 gaagacgaat cactgcataa atgaacacag aagccccgat cccaactcct atcacgctga
     9481 caaaaaaagc agtggaacga tgagttacag gaccaaatac tatattcaat acaccataat
     9541 acagcagcag tactactgtc atacaaagaa ggctgaacat caacttccgt ccaaaatgcc
     9601 gttctttcca aaaagcattt agctcttttg tttcaattcg aacaaaatac caaagcgtca
     9661 cacctaatcc taaaagggta gacaggcttg cacctgctgt tcctaataaa ccggtcagaa
     9721 aagaagtagt caatcctttt accaatagtc cccatcctgc gccttttaaa gaagggcgaa
     9781 acgagttctt gctttgagca atactttgat aagcttggat gactgcagtg aaagcaatgg
     9841 aaaaaacaaa taatacaagc gctgcatttc ccgcataatc tttaaacaac gcataattga
     9901 tataagggag cagcaaggct aatcccaaag acgcagctaa tgctaaagcg gtcgtcaatc
     9961 taagatagat tttggctgtc tgtaaaaact gacgatagat ccggttggtc aaatgacgag
    10021 tcaacgcagg caggaatgtc gcacttaatg cagtggcaat cactaaacct aattgtacta
    10081 aaggctgacc tcgatcatag accccttttg ctatcttcgc accgtattct gacagtccgt
    10141 aaacttccaa agcatttttt acaaagaaag agtcgatcag ctggaaaaag ataagcaatc
    10201 cactataaat cgaaaccagt ccgccttcta ccagaaaacg ccggatcaag ggctttgacg
    10261 gctgtttgaa caatggaaac cgtcggaagc ttaacgctcc tccatgaatc ttttgttcat
    10321 agtagtgaag gatcccatat gcgcataaac cgccaaaaac agctccactc attgctgccg
    10381 tccctgtttg gtacaccgtc catccaagct ttctaaaagc aaacgcacta cagacaatca
    10441 caaaaacacg aacgaactgc tcaactactt gagaaacagc agttggttcc atcaagaatc
    10501 ttccttggaa attccctcga taaaaagata gaccaggcat cagcaaaaag gtaaaggaaa
    10561 ctacttggat cagcggtttc aattgtatgt ctcccatcat ccaagcaatc cagctgctaa
    10621 atgcaaagac aaacaaccac aaaaagacac ctgtccaaaa tactaaggga taaagatttt
    10681 gcaaagcttg ctttcgctcg aaaggattct ttctttctgc aacgtacttt gatatgaact
    10741 gcggaagacc ggataaagct aaagtcattg ctatcccata aattggataa acttgctgat
    10801 aaacataaaa accttcgtcg ccgactaagt tttggagggg aattcggtaa agggcactta
    10861 atacttttgc tatgaaagaa gcaatcgtca atacaaatgc tccttgcatg acgcgccgca
    10921 tttctttatg atccaaatca ttgctccttc ctacgctgac agatattttt gctcacgcaa
    10981 cgctttgaca aacttttgga tttcctgaag ccatactgct tcttccattt ggttggggat
    11041 cgtcaagcgt atcaccatct gttctttttc aacgcctaga ctggctttta gttttgtcgc
    11101 tgtcagtgct tcgaataact gttccacact atagcgtttt gttccaactt tacttagagt
    11161 aaagactata tgcgcctcct gtttccttat cgtttctagg agcgctcgat cgccatccat
    11221 tttgatctgc ccaattgtca agagatgtgc tacttcttct ggatattctc caaaacggtc
    11281 gagcagatca tcttctagtt catctagcat ttcttgcgaa tcaagttcac gaattcgttt
    11341 atagatctcg attttctgcc gttgatctgc cacgtatgtt tcaggtagat acgcatcgac
    11401 accgaggtcg atctcaacag tggtcttctc cacttgacta ttctttcctt gtttacgaga
    11461 aacagcttcg cttagcattt gagaatacat atcgaaaccg actgcatcaa taaatccatg
    11521 ctgctgagca cctaagagat tacctgcacc gcgaatggaa agatcccgca tcgcaatctt
    11581 gaatccagat cccagttctg taaaatcttt gattgcctga agacgttttt cactgacttc
    11641 gttaaggatc ttctgctgct catacataaa gtaagcatat gcgacacggc tgcttcttcc
    11701 tacccgccct cgcaattggt ataacgtgga taatcccata taatcagcgt tctcaacgaa
    11761 caacgtattt gcgttcggga tatctactcc tgtttcaatg atggttgtag tgacaagaac
    11821 atcatattga ccttcaataa attcaaaaag cgtattttct aattgcgcct ctgtcatttg
    11881 cccatgagca taaccgatcc gtgcttccgg aacgagttct tggatttctt ctactttttg
    11941 ttcgatcgtt tctacccgat tatataaata gaatacttgt ccacctcgtc ccatttctcg
    12001 atggattgct tcgcgaatag ccccaggatt tttttccata acatacgttt ggatcggata
    12061 acgattcgca ggcggtgttt cgattactga aagatcccgc acgcctaaca tcgacatatg
    12121 caaagttctc ggaatcggtg ttgcagtcag agtcaaaaca tccacttgtg cacgtaactg
    12181 tttcaatcgt tccttatgtt ttacaccaaa acgttgttct tcgtcaatga tcagtagacc
    12241 caaatcgcta aattcaatat cttttgacaa aattcggtgg gttcctacaa caatatcaac
    12301 ttgacctgta cgcagttgtt cgatggtttc tttttgttgc tttcttgttc tgaaacgact
    12361 taatagcccg atatttacgg ggaaaccttc aaatctttcc agcatcgttt catagtgctg
    12421 ctgtgccaag atcgttgttg gaactaaaaa ggctacttgt ttgctttcct ttatcgcttt
    12481 aaaggccgct cgaagcgcta cttctgtttt cccataacca acatcaccta cgagtagtcg
    12541 atccattggt ttttcttttt ccatatcccg tttgatctcc gctgcacttc tcaactggtc
    12601 atcagtttca gagtagggga aggcattttc gaattctttt tggtaggcgt catcaggtcc
    12661 aaaagcatat cctttttctg actctcttgc ggcatataat ttgattagat cgtcagcgat
    12721 atcctcgatt tttgaagaga cttttcgctt ggttttcgtc cattcacttc cgcctagctt
    12781 attgatgcga ggcgctttgg cttcagaggc gacatatttt tggatcagat tcagttgagt
    12841 aacaggaata aacagtttgt cgtcgttttg atacaaaata gtcatatagt cttggtgaac
    12901 gccatctact tctagcgttt ccatcccaat gtatttcccg ataccatgat tggcatggac
    12961 aacgtaatct cctgctttta actcattata gcttttcaat cgctctgcgt ttgttactgt
    13021 ttgacgtctc gtttgttttt tcgtcgtttt ttggaaaatt tctttttctg tgatagctac
    13081 tattttttct tgaggaagtt caaaaccagc ctgcaaagct ccttcaacta attgtacctg
    13141 tcctctgatc aatgcatttt tttctgtttc tactactaat atgtcttcat ctcgaagcat
    13201 ttgttctgct ttgcgtattc tgtcctttgt cggaataaag atcacaacag tctgtttttg
    13261 cttgcgccaa cggtccattt ctgttttcag caatggcatt tgtccgaaga actgttgcat
    13321 cgtccgatat tgaaaattat ggattgcttg gaaacgaaga tttcccatcc ctttttggaa
    13381 gagtgagaaa aaggttgtgg tgaacttttc cttctgcacg attttttgta tatctgctga
    13441 aaatgtctgc tcagaaaaca cccgcaattc gctgattttt tgagtctgcc attctgccgc
    13501 ttccctttcg atttcccgat ttgtttccat gattcgctga taatcatcaa cgactagtaa
    13561 actatttgaa gggaaataat ccaagatcgt cacagattct tggtaaagaa gatctgagta
    13621 gaattttgct tcttctgtcg ggacacctgc ttgccagctt gaagtcagct gaccgaagta
    13681 atcttgcaaa aactcgcggt ctgcttcttc tgtggtcgca gcaagccgtt tttctagcat
    13741 tttttgcatt tgctctattc catgttctag atcctctggt ccgtagactt gttcactcgt
    13801 tggggaaacc cagatttctt ccgttttttc gattgagcgt tgtgtttctg cttcaaaata
    13861 ccgcatagaa tctatctcta catcaaatag ttctacacga acaggatagg ttgtcgttaa
    13921 cgggtaaata tcgataatgc ttcctcgaat actgaattct cccggttttg cgaccatctc
    13981 ttctcgtaca tagcccatca aagtcaattg gcgaggaaga ttttccacgt ctatctcatc
    14041 accaatatgc caattcaatt gcgcctgtag ccaagttttc ttattcggca gtcgtttgtg
    14101 caaagatgcg actggcaaca aatagatacc tgcttttttt tcttgaagtg cgttaagcgt
    14161 tgcaactctc tcgcctcgcg cttctggaga agaaaaggcc atttcagcag aaagcacttc
    14221 atccactgga aataaatgga tctgatcttg cggaatcaca tgttgcagat cttctgctaa
    14281 ctgattcgca taatacaaat ttggcatcag aataatcatg tttttatttg ttttcttaaa
    14341 cgctcgagca aatagaagtg ttttcgctga gcccgctaat cctgtgatta gttgtctagg
    14401 cattttttct tctaaattgc tgacccattc ttttacttgc ggatcttttc cgactaattg
    14461 aataatatcc attccttttc ctccttcctc aattgaatcg attcatcgca tccacgaacg
    14521 aatgcccttc tatgagatat tcgactgctt taacagattg acacatgctt tcttctatca
    14581 aaggctgatt ttctttgctg aaaggactaa gcacatgctg gactactgtt tttttccctt
    14641 ctggcctgcc gattcccacc ttgattcgat caaagacatt cgtattcaaa tgggaaataa
    14701 tacttttgat accgttatgc ccgccggcac ttcccttttg acggagccgg atttttccca
    14761 ctgccagatc caaatcatcg tagattacga ctaattcctc tggataaatc ccaaaataag
    14821 tcattagtgg gccgactgca cgaccagatt cattcatgaa tgtttgtggt ttgactaaaa
    14881 ggatcttttc tccattatga aaaaattcgg caacttctgc ttcgaacgga ttttttttga
    14941 aagtagcttg atgctcgcgt gcaattttat ccacgaccat aaagccgata ttgtgttttg
    15001 tatattggta tttggtacct gggtttccta acccgacgat cattttcatt tttgttttcc
    15061 acctaattct aaaaagcagc acaaaaggct ggactgctaa ttagcggtcc agtccatcga
    15121 gcttttttct tttttacact tgtcaatgca tagtataaca caaaacaagg cgtttcggct
    15181 aaaagagaat cgttaataaa tgataaagaa attaaaaatt tcttcaaagt ttaatgaaat
    15241 aattgatata taaaaatagt gaaataacgc acatgtaagc ttttacatat aatacaacct
    15301 tgttatctgt tatattttat agccaaatag cgtgacatgg ggcttgagaa atgttagcat
    15361 aagcatggga aaaaacaaat caatggaagg attgatacta aatgactgca gctgcagaaa
    15421 aaaaagatca tcaaaaagtt attcttgttg gagacggcgc cgtaggttct agctatgcct
    15481 ttgctctagt tactcaaaat atcgctcaag aagtaggtat tgtggatatc aatactgcta
    15541 aaactgaagg agacgcaatt gacttgtctc acgctttggc atttacttca cctaaaaaaa
    15601 tctattctgc tacttacgca gacgcacatg atgctgactt agttgttatt acagctggcg
    15661 caccacaaaa accaggtgaa actcgtttag acttagtaaa caaaaaccta aaaatcaacc
    15721 gcgatgttgt cacacaaatc gttgaatcag gatttaacgg tatcttttta gttgccgcta
    15781 acccagtaga tattttgact tactcaactt ggaaattctc aggtttccct aaagaacgcg
    15841 taatcggttc aggtacctct cttgactcag ctcgtttccg tcaagcactt gcggaattag
    15901 ttgacgttga tgcacgtaac gtccatgctt acatcttagg tgaacacgga gattcagaat
    15961 tcccagtttg gtcacatgca aatgttgccg gcttgcaaat ctatgaatgg gtaaaaaaca
    16021 acccagaaat cgatgaagaa gctatggtta acctattctt tggcgtacgt gacgctgctt
    16081 acacaattat cgagaaaaaa ggcgctactt tctatggtat cgcagtagca ttagcacgta
    16141 tcactcgtgc catcttagat gatgaaaatg ctgttcttcc tttatcagta ttcatgaatg
    16201 gcgaatatgg attgaacgac atctacattg gtgcacctgc tgttatcaac cgtcaaggta
    16261 tccaaaaagt aatcgaaatt cctttaagtg acagcgagca agaccgtatg gctgcttctg
    16321 ctaagcaatt aaaagaaatt ctagatgaag cttttgctaa actagacgct gaataatctt
    16381 tgaacatgac ggaaaacatc tattcggtgt tttccgtttt tttattttat cacccttatg
    16441 gtttctaaat tttttcataa agtagagata accttcgtct tttcctctaa attatgctat
    16501 catgagaccg ttccacccta aacttttact taaggagttg tcgatatgac aagatcttcg
    16561 catcgaaaaa aatcgaaaca tgttgctttg ctttcagttc ttggtgttct cttagtgctg
    16621 atcagctttt atacctatcg cagtacatac tacacaaagc attttttgcc gaatacagaa
    16681 ataaatggaa taaatgtcag caatctaaca gtcgaaaaag caaatgaaaa attaaaagaa
    16741 gcttattctg ataaaaaact ggctattaaa gaagatggaa aagtctggca ggaagtacca
    16801 aaatccgagc tcggctacaa agatgatttc acttcggagc tttctccgat cctaaatgaa
    16861 caaaacggat ggacttgggg aatgacttat gtctcagcgg cagaaaaaca agaaatagat
    16921 cctgtcagtc aaaaccacca aaaactagac actaccgttc agacattgac aactaagctg
    16981 actgatttga acaaagaccg gacacctact actgatgcaa cgatcgaaaa agcaggtgac
    17041 tctttcgtta tcaagcctga agtcaatgga aatacgatcg acgtcgatgc tgcggtcaaa
    17101 caattaaaaa gtgcggtaaa ctctggtaaa gatacaattg aattgactga attcaaagaa
    17161 aaaccaaaag taacatctga agacagctca ctgaaagaac aactcgcatc catgaatgct
    17221 atcgcaaatg taaaagctac ttacagtata aacggtgaga ctttccagat cccatcaagt
    17281 gatattatga gttggctgac ctacaatgat ggaaaagtcg atttagatac agaacaagta
    17341 cgtcaatatg taacagattt aggcacaaag tataacacaa gtacgaatga cacaaaattt
    17401 aaaagtacaa aaagaggaga agttacggtc ccagttggca cttacagttg gacgatccaa
    17461 acagacagtg agacagaagc attgaaaaaa gcgattctgg caggccaaga ttttactcgt
    17521 tcacccattg tgcaaggtgg cacaaccgct gatcatccat tgatcgaaga tacgtatatc
    17581 gaagttgatc tggagaacca gcatatgtgg tattacaaag acggaaaagt ggcccttgaa
    17641 acagatatcg tttccggaaa gccaactaca cctacacctg ctggagtctt ctatgtttgg
    17701 aataaagaag aagatgcaac tttaaaagga acaaatgacg atggaacacc ttacgagagt
    17761 ccggtaaact actggatgcc gattgactgg acaggtgttg gtatccacga ttcagactgg
    17821 caacctgaat acggtggaga cttatggaaa acccgcggtt cacacggctg catcaacacc
    17881 ccaccaagtg taatgaagga attattcggg atggtcgaaa aaggaacgcc tgtattggtc
    17941 ttctaacaaa aataatatat aaaaagaaag cacccgtacg attggtacag gtgctttctt
    18001 tttattatct aacaatgtgc tacttacatt tcagcagcag taaagacttc tgaaacatca
    18061 tcatcgtctt ctagcttatc tactagacgt tccaattgct ctttttgttc ctcatttaaa
    18121 tcaatcgtcg tttgtggaat catcgtcaat tctgcttgcg ccaaagtgaa ctctttttcc
    18181 aactcatctc gtacagcagc aaaatcttct ggtgcagtat aaatctcgaa tacttcagga
    18241 gaagtttgca gatcttctgc ccccgcctct aatacatcct caaacatcgt ctcttcatca
    18301 acagtcaacc catcgcgttc aatagcgata tatcctttgc ggtcaaacaa gtaactgact
    18361 gaacccgttt ctcccagtga tccaccgttt cgagtaaaag ctactcggac atttgtcgct
    18421 gtccgattac ggttatctgt caatgcatgg actaagattc ctactccacc cggaccatat
    18481 ccttcatacg tgatttcatc gtaattcgtt tcatctacgg cactcgtagc ctttttaatc
    18541 gcccgttcca cattgtcgtt aggcatattt gcagcttttg ctttgtccac aacaagccgc
    18601 aaagctggat tcatagatgg atcaggaccg cctgctttag ctgccatata aatttcacga
    18661 gaaagtttct gaaagacttt ccctcgtttt gcatcttggg cattttttct tccttggata
    18721 ttatgccatt tactatgtcc tgacatgatt tttcctccct tgttcttttg agaatacact
    18781 tctattctag tactatttca caaaaaaaca aagccacagc caatctataa acgcttcttg
    18841 aatgccgcaa aaagcacaca taatatcaca gcagtcagag caccgatcga atccaacatg
    18901 acatcttgga acataggtgt tcgtccaccg gtcatcatct gatggaattc gtccaaacct
    18961 gcgtaccctg ttgctgaaag ccaagcaagg atcgctgtca gccaccaaat ttttagcttg
    19021 gggaaaacac ctaaaaacaa gctgcctccc aaaataaaat aggtaccgaa atgtgcgccc
    19081 ttccttaaaa agaactctaa aaatttggcg tagcccagat ggttgatact caccatactt
    19141 ccaccataat taaaacgtat acccgctaat gtgtctttaa atggctgatt aggcagccag
    19201 ctttctaatc gcgaaacttg tgattgctgt ccgtaagttt gagaagaact gtaaaataag
    19261 attataacga ctatcagtgc gattactaaa taaatatttc cgtttttcat ttgttttttt
    19321 gaagaattca taagtcacct ctgtatttta tcttagactt tttttgaaga aacaacaatc
    19381 attttcatag agacttcttc cataaaaaag tgctatactt aattttgtat tacaatatga
    19441 agggagtttt ttcatgagtt attcattaaa ttggaatcta gattctattt tttccggagg
    19501 cagtcattct gatgctttga accaacggat gaagcaattg gaagaccaaa tgaacgagta
    19561 ttatcaccgc gtaacaaaat ggtcacctag ttctgacaat gcagagcagt taaacgctat
    19621 tcttcaattg caagaaacga ttacgaatgg ttttacccaa tgcagcagct atattactgc
    19681 attgctttct gccaatgtca atgattcaga tgcaaaggtc ttatcaggca agctttacgc
    19741 aatgctgcct cgtctgcaat cagccgaaac tgtattgtca aagaaatttg cagaaatatc
    19801 tgacaatgat tggaatcaga tgctttctca atcttcgttt gaaacaattg cttttcgttt
    19861 aaacgagatt cgtcgtgatg gctcacaatt attatcagaa gctgaagaaa acattataaa
    19921 tacgctttct ttagatggtc taaatgcttg gagcagtcat tatgatacga ttgtagccag
    19981 catttcgatt cccttcgaac aagatggaca agtagtcgaa ttatctgctg gacaagcctt
    20041 caataagatg atgggcgatc cagatccaaa agtcagagaa acacttttcg cagcatggga
    20101 agaagcctgg aaagaaaagg cacccctctt cacggatact ttgaatcatt tagatggttt
    20161 tagactttct gactatgaac ttcatggtgt cacagatttt ctacaaaaac cattagagta
    20221 caatcggttg aagaaagaaa cattgtctgt catgtgggat acaatccaga aaaacaaaca
    20281 accccttgtt aattacttga ctagaaaagc taatcttttc ggtaaagaaa aaatggaatg
    20341 gcaagatcaa gatgcaccta ttattttagg cgatttgaaa gaaaaaacct ttagttttga
    20401 cgaagcagca gcatttatta tcgaaaactt taacaaattc agtccaaaaa tggctaaatt
    20461 tgcccaatct gcttttgaaa agagctggat cgaagcagaa gatcgtcctg ggaaacgtcc
    20521 tggtggttat tgtactgagt taccagaaac aaaagaatca aggatcttta tgacctacag
    20581 taattctgtt aatgaggttg cgacacttgc tcatgaacta ggacatgctt tccatagcag
    20641 cacgatgtgg gatttacctt ctttaaatcg cgaatatgcc atgaatgtcg cagaaacagc
    20701 aagtacattc gcagaactga tcgtagccga tgctacatta aaagtcgcaa aaacaaaaga
    20761 agaaaaaatc aatttgctag atacaaaact gcaaaatgct ttagctatgt tcatgaatat
    20821 ccattcccga ttcatttttg aaaatcgatt ctatgaagca cggcaagaag gattagtctc
    20881 agaagataag atcacagaga tgatggttga ggcgcaaaaa gaaggctatc aaggagcttt
    20941 agctacttac catccatatt tctgggctgc aaaattgcat ttctttattg atgacgtacc
    21001 gttttataac ttcccataca catttggcta cctctttagt ttgggcatct atgcttacgc
    21061 caaccaaaag ggagcaaatt ttgaagaaga atatatccat ttactacgcg atactgcctc
    21121 aatgacgacg gaagatcttg cgaagaaaca tttgaatgtc gatcttaccc aaccagattt
    21181 ttggcaagca ggtatcgaca tggtgactga agatatccaa acgttcatgg aattgacaga
    21241 agaatttatc tgatttctat tttatcacaa taaaaaaaga gttagtccag aatgggctaa
    21301 ctctttttga ttaatcattc ttacatgctg acagcaaagt caggggcaaa ccattgaact
    21361 gaaccaactt ttacgctttc gcctggttga ggagcatgga tatattgtcc tccgcctaaa
    21421 gcaatcgcta cgtggtaagt tccgccttgt gacccccaga ataacaagtc tccagctttt
    21481 gcttgagaaa ctgaaatttt tgttccagct gattcttgag gaactgtcca accaccgatg
    21541 tcacgaccag ttacttgcat gtaaacatag cgtgtgaacc ctgagcagtc aaagccactt
    21601 ggatctttac cgccccaaac atatggagta ccaatatatt tgtaagcttc tgctacgatt
    21661 gctgcaccat tcacacttcc agaaggagct ggagcaggtg ttacaggttt tggttgttca
    21721 ggtgttggtg ttggcgttgg agccggagta ctacttcctg tgccgtttcc tgtatcaaca
    21781 ctttgatctg ttgatggtgt tgtaggcgca ggtgttgttg attcttctgt tgttgaagat
    21841 tcaggcactg ttgttgattc ttctgttgct gaagattcag gcactgttgt tgattcttct
    21901 gttgctgaag attcaggcgc tgttgttgat tcttctgttg ctgaagattc aggcgctgtt
    21961 gattcttctg ttgaagattc aggtgttgtt gattcttctg ttgctgaaga ttgtgtagct
    22021 gatgatgatt cttcagtcat tgaagattgt gtagctgatg attcttctgt tgctgagctt
    22081 tgtgcagtag atgaagcttg agtagcttgt gcttcagcct ctgcttgttc gcgtgcttct
    22141 ttttcagctt tttcttgtgc agcttgttga cgagcttgtt ctgctagacg ttgttgttca
    22201 cgaatacgag cttgctctgc ttcagcttct gctttttgac ggttcaagtc agctttcttg
    22261 tcttcagcag ttgcttgttc agcagctaat gaagttttca gtacgttcaa atcagcttgt
    22321 ttagataaaa gatcgccttt ttgagattcc aaagcagctt ggttttcagc taattctttt
    22381 agtttagcat cgttttcagc ttttttgtct tctacagctt ttttatcttg tttttgttgt
    22441 tccatcaagt cgttgtttgc ttttaccatc gttgtcattg cttgaacgcg tccaatagca
    22501 tctgctaatg aatcagcatt caatacagca tcgatgtagt ttgagcttgt gttgcttact
    22561 tgtgcttcgc gtgcttgttt ttggattgta tcttcacgtt tttcaatacg ttcttgtaaa
    22621 tctgcaatgt cttttactaa ttgtgcagat tcttgacgca atgtatcttg ttttgctaat
    22681 aggtcttgtg cttgtgtgtt gatttcagaa acttggcttt ctaaggcatc gatttgtgat
    22741 tgtgcatctg cttgttgatt tttcaagtct gcaatctttt gatcttgctg ttgtatctga
    22801 gaatcaaaat cgtccgccgc ggcagcgatc ggtgatgcta cggcagtcaa ggtcattgaa
    22861 cataccatta ctgctgatat taaactcttt ttcactcttc attcctccga ctggcttaat
    22921 ttaataaata tctaaatata tttaagtctt tcttaacgat ataaaaattg taccatagca
    22981 gtgtgtcaac gatataatag ttatgttaca aaaatgtttc aaaacaactt ttaaaaatga
    23041 caaaactgtt cgtttcagaa aatgatcatg aaaaagccgc gttaagtcta gcatttctta
    23101 agataacaac gattcttttt cgctttcgac ctaaaaataa aaaaagaaat tattcatcac
    23161 tgaataattt cttaaaggga aaaatgaata atacaaatat aatcatgttc aacagcagtg
    23221 taggtcctaa aaaccttgta ataaaatacg tgttatttac tactgctaat ttaaaaatca
    23281 actggaccac catcgtaaac aattcataac ccgtgacaaa gatgatcata ctgaaaaatt
    23341 cagtaaagat gttgacatgg ataacgtctt tcattacata catcaaccat acaattaaag
    23401 ggagagctac tgcatagata ccgataacgc caatatagta agcgtcatag atagccccga
    23461 gaacaatcgt ggtaatcagt aaataacgtt tttcaaacgc catactgcaa cataataatg
    23521 ccaatatcag aaagtgggca tttgacatat aggtgccttt cgaccactcc cctagcattc
    23581 ttgttaaatg accatctatc aacatcaaaa agaacaaaac gattggcaga tagtacttca
    23641 ttgtttcttt cttcaacatg gactaatcct ccgctagccg ttggacaatc gtcacaactg
    23701 acacgtcata catttctgca taaggtttca cataaacttc ccgatccagc ccataactat
    23761 ctggtttcgt tttaataacc gtaccgatcg gcaagttagc tggagaattc cctccaagac
    23821 cggatgtctg gacaacatcc ccttctttga tatccgtatc accagttaat tgggtcacca
    23881 ctaaggcatg gagcttttca tcatagtttt taagcaaacc aaacgcttcg ccattagccg
    23941 aagatacccg tactggaaaa tgattggagc tttcattaga ggatgacagt aattcgattt
    24001 tagacgaagc cgtattgacc tcgatcactc ggccaatcaa tcctttttgc gacatgactg
    24061 ccatatttgc ttcgataccg tctttagaac ccttgtctac gatcagcatg tcctgccaac
    24121 tgtcaggcga acgagtaatg acatttgccg ttactttttc gtaactagtc agcgtctgat
    24181 tcaactcaag ttcttttttc aacgcttcga tttctctctc gtaattcttg ttttgctgtg
    24241 aaacgtcatc atagctatcg attttttctt ttagccgctc attttcggca taagttgaaa
    24301 aaagattatg gacagaatct actccatttc gtacccaccg tactggggca gagattgttt
    24361 tatcaaccat tgcaacactg tcattaacga tggattggaa gaaattgggc tttccttcat
    24421 tcgctcgctg cgcagcggtc acacttagta tcgtcaccac gatgataaca ataaccaagg
    24481 taataataat atttttatta ggattgaact tcttcacaaa gacacctcga attaattaga
    24541 ctcttgtgtt caattttatc actaaatgcc gtgaaacaaa aggtaagttg ctcctcttaa
    24601 gctttttttt agcaaataat agattctgtc atttttagcc tgaaagacaa atttttttat
    24661 ttgtttatgg atcaatcttt gctttgaata actgcaatca taccatctga aaaagaaaat
    24721 tctgcggtat ctgaaagaaa ttcgttactt gcataagatg tcggatattc tacttcttta
    24781 ttttccaata catacttgct gccttttaat gtaagattcg agacgggagt caagcagcaa
    24841 taagctaaat acttcatatc tggtatctgt cttactatat gctctccagg caaataataa
    24901 gacaaataat tttgacaatc tctgataata agctgatggg caaagggttt gaagcgaggt
    24961 tccataccta accagagatt ggctaaaaga tgatccaatc ttccgcccgt agctccaatc
    25021 aaagtgactt ccgcttgcgg gtaacgttca aatgttttta agatacctaa ctgtgtatct
    25081 gtatcgtctt tttctgcagg ggaacgggta tactctttgg cttttgcaaa tacttccttc
    25141 tgctcttcgt tcgacaatga atcaaaatca cccactgcca gatctactgg gtagcctgct
    25201 tgaagcaggt acagcgcacc acggtctatc ccgacatagg catcgtattt ggacagcaga
    25261 gactgcggcc agttttgcgg attacctcca gcagctaata aaattttcat ttagtctaat
    25321 gcctctttta atcgttcgat ttgtccgact ggattttcgg ctccatagat atatgatcct
    25381 gcaacgaaga catccgcccc tgcatcttta cagatttttg ctgtttctgg aacgattccg
    25441 ccatctactt cgattgcata agtccaattg ttctctctac gtagatcagc gagttccgta
    25501 attttttcta ctgtttcttc aataaagctt tgtccgccga acccaggatt gacagtcatg
    25561 actagtactt gatcagccaa aggcaaaaca tgtttgatca tggagactgg tgttcctgga
    25621 ttgataacta ctccagcttt aactcctaag ctttttgtca tttgaagtgc ccgatgaata
    25681 tgaggagttg cttcgacgtg gacagtaatg atatctgctc cagcttttgc aaatgctgga
    25741 atgtagtttt ctggatcaac gatcatcaga tggacgtcta acggtaattt agtaacagga
    25801 cggattgcct gaacaatatt tggccctaat gtgatatttg ggacaaatcg gccatccatt
    25861 acatctacgt ggatgtaatc tgcgcctcct ttttccacta actcaatgtc tctttgtaaa
    25921 ttggcaaaat ctgcactcaa aatggatggt gctatcttca ttcttgtttc ctcctatttt
    25981 cgtttacctt ctttttttgc atacattggt tttctttttt cgatttcttg taagaattgc
    26041 aaataattgt cgtaacgtgt ctgtgcgata ccacctgttt ctactctatg ttttacttcg
    26101 cagccaggtt ctttagcatg catgcactct ctaaatttgc aatggacaga agcttctaca
    26161 aattcaggaa attgtctcgg cagttctcct gcttcaattg tcaaaaagtc aatcgagcta
    26221 aatcctggtg tatctgctac taacccgcca tagataggaa tcagttccac atgacgtgtt
    26281 gtatgccgtc ctcttccaag tgcatcagaa atctctcctg ttgctaaatt cagttccggt
    26341 gatatttgat tcagtagcgt cgattttcct gctccagact gacccataaa gaccgatagc
    26401 cgttctggga agtaacggat cagttctgtc gtatcacccg tatgattggg tacaatcacc
    26461 ggatagccta ttttagtata ggtttgcttg atctcttcga ttttttctgg gtcgtctgac
    26521 aagtcggtct ttgtcaaaaa aataatcggt tcgatcgact cgtattccaa ggtaactaga
    26581 aagcggtcaa gtagattata agaaaatgca ggctctacga tacttgtgac aatgattcca
    26641 agatcaacat ttgtgactgg tgggcgaact aattgatttt ttctcggcaa gatttccaga
    26701 agatagccgt ctgtttgatt ttcactctca aagatcacct gatctccgac aagcggcgtg
    26761 attttccgat tccggaaatt tcctcttcct cttgtttgat aggtctctcc gtttgcgtat
    26821 acatagtaaa aaccgctgat tgcttttctg atttgtcctt tgataagtgc cacctccgtt
    26881 tttgtttatt ttaccatacg aagaggaaag taggtgttat tccatgcaaa cttctcaaag
    26941 tatatggctt gaaaattgat caaaagagat agaaacacaa aaaagagaat gcaacaaatc
    27001 aatgtcgcat tcccttctga tatattttat tcagcactta ttttgaactt tctgaggtgc
    27061 tagctgatga acttgtggag ctggaagatt cttcttttga agagttgctt gtgctactgg
    27121 agctacttga gctgctagaa ctgtctgaag agtctttttc agaactagaa gacgctggat
    27181 ctggtccttt agagtaaatt acgttcacaa ccattccttt ggtaattgtc gttcctgccc
    27241 ctggtactgt acgaatcact ttatctttat ccaccgtgtt tgaatattcg gaagtctcat
    27301 gcccgatata ttctgcaccg acactagcta aataactttg tgcttcgctt tttgtatagc
    27361 cggagatatc attcaattta ggctctgttc cttggcttac gacaaaattg atgtaagttt
    27421 cttctgctac aacttcaccc gaggctggat cctgactgat gatcgaacct gatggtacag
    27481 tgtcgctata gtcataagta gcactaatat tattgatatt gaatccttgt gtaacaagcc
    27541 aattacgaac agtttcgtag ttttctccta catgactact caaagtcact gtttctggtc
    27601 ctttgctgac tgtgaaagta atcgtgtctg ttttaggatc gacttctgta tcttctgatg
    27661 gagattgcga aatgatcatt ccgctttcta cctctgaact gaattcctct tctttttgaa
    27721 tcaagtcagc agaaaaccct aattctttca aacttgcttc ggcatcttca taacttttac
    27781 cactgtaatc atctaatttg atttttttcg tgccgctact aatatacaga gtgacttcgc
    27841 gtttttgctt gactgtcgtt cccgctgccg ggtctgtctt cactacatta ccttcttcga
    27901 ttttgtcatc tgcaatttct tctgtttttg ttgctacttc taatttcgct tttgcgagtg
    27961 tttctctagc agaagcttca gaaagatcag aaacatcagg aacaacaact tctcctcgcc
    28021 cgccagaagc aaagaataca gctgttccga ttcctaaaac agcaagaata gctaatagga
    28081 tcagccacca ttttttctgt cctttctttt taggatcagg cagaacttct tcctcctctg
    28141 gtaaggtttc gttttctggc ttgtcttttg gcagaggcat agatttgaat gaatcaggca
    28201 ttggtgcatc ttccgtaagg ggtgtgataa cttttgtttc attatccatc acatgaggct
    28261 gccatttttc ttcgtttgca cgagctggtg ataatactgt agaaagatca tcagacattt
    28321 cctcggctga cttataacgg tctgcaggtt ctttagccgt tgcccgtaaa atcacgtttt
    28381 ccagtgcttg tggcacattt ggatcaagtg cttttataga tggaagatca tcttgaaaat
    28441 gttttaaagc aattgttaca gcagattcac catcaaacgg tacgctaccc gtcaacatct
    28501 cataaaggat gatccctaaa gcataaatat ctgactgctt cgttgccata cttcctctgg
    28561 cctgctcagg ggacagataa tgaacagatc ctagcatcgt attcgtctgc gtgatcgatg
    28621 tttcagataa agcgatagca atcccgaaat ccgtgatttt tacaacacct tcattatcga
    28681 tcagtacgtt ttggggtttc aaatctcggt ggatgatttg atggttatga gctaaagaaa
    28741 tagcagaaag gatctgctgc ataatattta ctgctgtttc ataaggaaca ggataatgtg
    28801 tttggatata tcgtttcaag tccattcctt tgacgtattc catcaccaga tactgcatgt
    28861 tgtcttcttc accaacatcg taaacactaa caatgtttgg atgaacgagt tctgttgctg
    28921 ctaaagcttc tctctgaaat cgtcgaatcg cagtctggtc attttgaaag tcaaatcgta
    28981 agactttaac tgctacatca cgatccaaga ttaaatcgtg cgccaaaaat acatttgcca
    29041 taccaccgct accgatattt ccagtgattt gataacggcc atttaatttt ttgcccaact
    29101 cgatcataca ctgtcctcct tatattcgat aagaagaacg gtgatattat ccgctccgcc
    29161 tgcttcattg gcacgatcaa tcagctcagt tactttatga gacaatgggc cttcttgatt
    29221 gatgatcgtt cctatttctt cttcagacag catgttcgtc aagccatctg aacataacag
    29281 cagtcgatct tttagttgcc aaagatgagt agaaacatct acttcgactg ttccaggcat
    29341 gccgacagaa cgtgttaaaa tatttttcct tggatggttc actgccattt cttcgctgat
    29401 ttctcctgat ttgactagtt cattgactaa agaatgatcc tccgtcaatt gcatgatttt
    29461 atcttcacga atcaaataag ctctgctatc cccaacattg gcaatcgtga accagtcatc
    29521 tgacagagcg gccgctacga ttgttgttcc cataccgtta tattccggct gttcttgtcc
    29581 acgctgataa atcagcatat tttcttcttg aatcgattga atgaaccatt gggcgatttt
    29641 ttcatcgtca acagcttctt gtttttccca agcttcgcct agattcgtta ccgccatctg
    29701 actggcaatg tctccggcac gatgaccacc catcccgtct gctaatacag caagcttgag
    29761 acctgaacgg ctggtaaata cactggcata gtcttgattt gtgttacgtc gtcttcctac
    29821 atctgattga tattcaatct gcattttttc acctcattac tttttgcgca aacaacaaat
    29881 gaaaaatcca tctgtgtaaa actgatgcgg atacaaagtc aacattttct cttcgattga
    29941 tgattgtagc ataggattgg tcaaaacatc aatcctctca aaatcagggt gtctttccaa
    30001 gaaagcagaa acaacgtctt ggttttcttc ttttaagatc gtacaagtgc tataagtcag
    30061 aataccggaa gactttaaag ttggtgcgca gctttctaaa atttccaatt ggatttttgg
    30121 taaattcgct aattcatccg gctgtttttt ataacgaata tctggttttc gccgaagcag
    30181 cccaagacct gaacaaggcg cgtctactaa aatccgatca aactgctctg cttcgaactg
    30241 tttactgact tctctagcat ccatcttttg agcgtagaca gcagaagaga cacctagacg
    30301 tgctgcatta tcttcaatga gtttgacttt gtgttcgtgt acatctaaag cgaccacttc
    30361 tccaccgcat gacggatcta aaaaagaagc aatatgcgtc gtcttacctc cgggtgctgc
    30421 acaggcatct aagacatgat gttcaggctc gatttgcata gacggcgcta caagcatcga
    30481 actttcatct tggatcgtca tgacgccatc gtggaacaat gagctagaag ctaaatgccc
    30541 ttttttcgct actataccgt atggactgac ttggcttttt tcagctgtga tctcttcttc
    30601 ttgcaaaaca gtgatggcct gatctctgga aagtcggcgt gtatctactc gggcactagc
    30661 atggctaggc tgatatagcg ataaaccgag cttttcagtt tcttcgtatc cgatttgatc
    30721 caccagtcgt ttcgtcatcc atagaggcag gctgattttg acagaaagtc tttcgatagg
    30781 atcagcaatg ccttgcagat ctggtgctcc ttgacgctga taattcctca agacaccatt
    30841 tacaaatttc cctgtcccag gatttccttt tacttttgcg atttcgaccg cctcgtttaa
    30901 aatagcatgt gcagggactt tatctaaaaa ttctaattgg tatacagaaa gataaagaag
    30961 catcttgacc cagtcatcca ctttttttgc tttagcgatc aatggtgcaa cgtaaaattc
    31021 caatagtcgc tgatggctaa tcgtaccata gatcaattct gtaaataaac ccacatcttt
    31081 ttgatttagc tcgctttgtt tgatcatctc attcaacaaa agattcgagt aggcgccacc
    31141 cttgttcact cgctctaatg tcagtaatgc catatatcgt actgattttt tcattttttt
    31201 agggattttt ttcattattt cacctgctga ccaatctgaa cttgctgacc gcttccgtta
    31261 aggaaatcat gtacggcctg ttttccttta ccagctggct gtaattcatt gatggctaag
    31321 acggtctgtt taccgcaagc aatccataga ttttttttgt ctttccggat gatcgtacca
    31381 ggtgctttgt ctgtagtttc tgccaatact tcaaccgata ataatttcca gcgtatgccc
    31441 tcataggtcg tgaatgcaat tggccaagga cgcattccgc gtacttggtt gtcaatttct
    31501 tctgcagttt tatgccaatc gattgcttct tgttcccgtg tgatgtttgg cgaaaaagtt
    31561 gcttctgact cgtcttgagg tactggtttt tgtccatcca aaatattggg caatgtttct
    31621 agcaatagct ttcttcctaa aaggctaagc ttttcaaaca tcgtccctac atcatcttgt
    31681 ttagtgattg gtatgctttc ttgcgcatag attccgcctg catccatttt tttgatcatt
    31741 tccatgatcg ttacaccagt ttctttctcc ccattcatga tggcataatg aacaggcgca
    31801 cctccccgat atttaggaag taaagaagca tggacattga ttgcccctaa ttttggtact
    31861 tgcaataact tttcaggcaa gaattggcca aatgccgcag tgacgatcac atcaggcacc
    31921 aattcaatga tttcttccat ttctggcgaa ccggagattt tctctggctg cagcacacgg
    31981 ataccgtgct tcagagccgc tttttttacc ggtgtcggcg taattactcg tttccgccca
    32041 actggacggt cgggctgtgt taccactgcc tggatctcgt aacctgattc tatcagccct
    32101 tcaagaatcg gcacagaaaa agcaggtgtg cccataaata ctagcttagt catcaagatg
    32161 ttcctccata tattcttcaa gttcttcttc cgggatctgc tgtatcattt tctcaatgaa
    32221 caagataccg tctaagtgat cgatttcatg ctggaaggca cgggctaaat agccaaaagc
    32281 cgtcacttct atctcttctc cgtctcgatc ataataacgc acggtcactt catctgctcg
    32341 cttgaccgtt ccatagacat gcggaatact gagacaacct tctacgtcaa ggctttcacc
    32401 ttttgcttcg atgatttctg ggttaatcag ttcaaatttc tcgccttcat ctacttcgat
    32461 cacagcaatt ctcttgtttt gtcctacttg aggagcagcg atccctattc cgtcattcgc
    32521 aatcattgtc tcatataaat tgtctaataa agcgattgtc tcgtccgtga ttacatcgat
    32581 tggctgagca gttcttttta atttatcatt agggtggatc aatataggat agcgcataat
    32641 ttggcccttt cttaaataaa gtttaatggt tcgttgtcaa ttgccacaaa aagaccttgt
    32701 cgctgttctt tctggcttcc attcaaaatt tcatccaaaa catgaggaag attcggttca
    32761 tgtttatatt tgatgattag ttgataataa tatcggtttt tcactctcaa aatcatgctg
    32821 ggcgttggcc ctaatagcaa gctttgaggg cttaacactt cttttagctg attggcaatc
    32881 tgaaagattt tctttgccgc tacttgttct tcaggatggc tggcggttat tttcacagta
    32941 aagtaataag gaggatagcc tccacgatgc cgtacatgca tttcctgctg ataaaactgc
    33001 tcataatttt gttttttagc caattctatg gcgtaatgtt gaggattgaa tgtttggatc
    33061 acgacttcgc ctgctttttc cgctcgccct gctcgtcctg aaacttgggt aagcaattgg
    33121 aatgtatgct cactagaacg aaaatccggt aaattcaggg aggtatcagc attcaacacc
    33181 ccaactaatg tcacttctgg aaagtccaaa cctttagcaa tcatttgtgt ccctaataat
    33241 atatctgctt cttttgcacc gaatttttgt aaaatctgtt cgtgtgcccc ttttcttcga
    33301 gtggtatcta catccatacg caaaatccta gcttgcggat aaagttctcg gagttcctct
    33361 tctacttttt gagtacctgt tccatagtag cggattttat tgccgccaca attaggacag
    33421 cgattgggaa taggttcttc atgtccgcaa taatggcagc gcattgaacg tgtatccata
    33481 tgcagtgtaa gagagatatc gcaattagga catggaagaa caaatccaca gtccctacac
    33541 atcacaaaag acgaatatcc tcgacgattg agcagtagga cagtttgttc ttttttgtct
    33601 aatcgatttt gtattttttc ctgcaaatta gcagaaaaag tagaggtccg atgattctga
    33661 aactcttcac gcatatctac gatctcaata gctggaagct gtgcagctgc tttggctcgg
    33721 tggtttaact gcaataactg ataaacgcct ttttgagctc gtgctcttga ttctaaagag
    33781 ggcgtcgcgc ttcctagaac tattgggcaa tgatgatatt tactgcgcca aattgctaga
    33841 tctctcgcat gataacgcgg tgtctcatct tgtttgtaac ttgattcatg ttcttcatcg
    33901 ataatgatca gcccgatatt tttcagcggg gcaaaaatag cagatcgtgc gcctactact
    33961 acttgcgctt cttcccgctc aatcttgcgc cattcatcat atttttcacc ttgggaaagt
    34021 ccgctatgta agaccgcaac tgattctccg aagcgtcctt tgaatcgctc gaccatttgc
    34081 ggagtcaatg cgatttcagg cactaacata atagccgttt tattttctga caaaacatca
    34141 gcgattgcct gaagatatac ttctgttttc ccgctgccag tgattccctc cagcaaaaag
    34201 acctgatctt tttgctgctg acctgctgtg atgatttgtt cgacagcatt tttttgttct
    34261 gcattcagtt ccaatgccgt cgtttggtca aatactcggt ctttataagg gtcgcgataa
    34321 cgttctgatt ctataaactc cagccagccc ttttcttttc cttgattcaa gacagcagta
    34381 ctgattcctt tatttttgaa ataagcaatt ggttcttctt ggctcagttc agataaacac
    34441 aaaagaagtt ctttttgttt cttggcattt tgccgtaatt ttgcccattc ttcttcgatt
    34501 tgcttttcag ttaacaaaga acggaccaaa cgaatcgttt ttactttatt tcgtgtatgg
    34561 acttcatatt tgatatctac taattgctct tggcgccagc gaatcaattg agaaaggctg
    34621 ccttcttctt ctgcttcctt ccaggaaatc acttctcgct gatcaaacaa tacctgaact
    34681 tctggtgtgt ctgccaaagg atagatcaat ttatcatatt ctgctttcat cacactcgga
    34741 agcatcgttt gtagacaagt gattttgaat gcgtaagtaa tttttttcat ataatcagct
    34801 aatgaaagta attcttcatt gaccaccgga gcaaggtcta gcaaacgaat aactgccttc
    34861 aaagatggat tagctgattc ttctgatttt tggatagcca taacaaatcc ttggacatga
    34921 cgattgccat taccaaaagg gacttctact cgcatcccga cttgcaaagc tgtttccatt
    34981 tcagagggaa ccaaataagt gaatggctga tcggtctgca tagtaggtac gtccacaatg
    35041 acttcagctg ttaatgtcat ggttcgtttc ctcctttttt tgtcttttct tatcaagcga
    35101 caaaaagttc cttgcctatt ttactagaga tcgatggatt tgtcttatct tctgccaaat
    35161 cctaacaaaa aattaacagc agataagaaa aaaaagaact gtgtcaaaaa gttcagacac
    35221 agccttttct accagacaat aagtggcgaa tgtttgaaaa ggaaacagct gggtttgctt
    35281 ttctttttag atgttcgctc tatttttctt tattacattt tttgctcttc acggatacgt
    35341 gattccaatt cgtgttgttc acgctctttt tgtgcttgac gttcctcttc ttccatcttc
    35401 aaacgttcac gttttaattc aggatgtgga tcattgacta catttcctgc atcgatttct
    35461 tctaaagctt gaccgacgct ttttactgaa tcaaaagaat ctagagtagg ctgtgcactt
    35521 gcatccaatt catgcgcgcg tttacttgct aaaatcacta gagaatattt tgaatttaca
    35581 cgatctaata gtgagtcaat tgaaggtttt aacatcataa gactacatct cctttaacat
    35641 tttaatatat ttaccgatta cgcgatctac tcgaaaatgt tcactggcaa taatgttttt
    35701 gattcgttca actgctagcg gcacttcatc atttacaaca gcatagtcgt aaagagccat
    35761 catttcgatt tcttcttttg caactttcat tcgtttttcg attacttcat ctgcatctgt
    35821 tcctcggcct acgatccgtg attttaattc cgctaaatct ggcggagtca aaaagatgaa
    35881 tacaccatcg ggcacttttt ctttgacttg ttttgctcct tggacttcaa tctccaagaa
    35941 aacatctttt ccttcatcca atgtcttgtt gacatatgac aaaggagttc cataataatt
    36001 cccgacatat tctgcgtatt ccagcatctc tccagcttcg atcattgatt caaactcttc
    36061 tttgcttcgg aagaaataat caacgccttc gacctcccct tcacgcattt tacgggtagt
    36121 cattgagatc gaatactgaa aatcattatc ctcactctca aaaattgctt ttcgtactgt
    36181 gccttttcct acaccggatg gaccagacaa cacgattaat aatccacgct ctgacatgac
    36241 gacgtccttt caactagtct ttttgtactt atcgtttatt ttgcactttt tttctgcaac
    36301 tttcaaggtt atctttaatt atagctagaa attggtaaaa aagctaccct gtaccaagag
    36361 gtcgtaaaaa agctgtcctg catcaagtaa taagaacagc caaacataaa cagcttctca
    36421 tgtttttcgt ttggttgggg ttattatcgc agatgcaagc ttttgaacgc cgtgtataag
    36481 aggtcgtgaa gaagccgatt agtttcgagt atgaagaaaa attttgtgaa aatagtttct
    36541 cacatttttg cataaatttg gcttaatacc gagaaaatgg tttttgaaca tcacttattc
    36601 aaaataaaaa aagattgtga cacgaatttt gtcacaacct ttaaatgtat tcctagagca
    36661 aagaagctaa gcgaagaaat ccaattctgt ttatttattg tatttctttg ttcagcatcc
    36721 ttgctaaaag cccatattgg acaaagtatt cactacaccc atgatctcat cttttcgaga
    36781 acgtgctttt tctgtcggat cttctacctc ttctgtgtca attcgaacaa taaatttccg
    36841 attcatcgtc acgtaagcac cgtcgttgat caaaataaac agttcgttct cattatagtc
    36901 tgcacaggca tctttccata cgtcaaagcc gtttttttca gaagggatat cccgtttaac
    36961 cacagttttt ccacttaatt cgtggaacgt tacgtcataa tatttcatta tctctacctc
    37021 tttcgtcatt caatattttg tacttgttct ctgatttttt ctaagatcgt cttagactgg
    37081 acgacttgat ttttaatttc gattgcattt gatttggaac cgatcgtgtt gatttcccga
    37141 ttcatttctt ggatcaggaa atctaattcc cgacctacag gacattgtgc ttcgataagt
    37201 tcatctagtt tttgtacatg gatcgccaga cgatccagtt cctcgtgaat atctccgcgt
    37261 tccaacaaga tggctagctc cgtcaacaac cgttgctcat cgactttcgc tccaagaaat
    37321 tcctccagct ttttttgata ccttatttga aattcctttt catagatttc tacaaaacta
    37381 tcgagttttt ttagtacttc tttgaattct tcagtattct ttttcaacac taaagctaaa
    37441 gcttgacctt ctttttctcg actcgcctca atagaatcaa gtgcgctttt caatgtatct
    37501 agagctaact gtgccaattc ttccatgctc tcttccggtt tttcttctac cgttacgaag
    37561 ttttcgtcca gcacgaagcg ttccgccatc cgactaattg acacttgatt ttgactaccg
    37621 tatcgttgtt gaattccgtc tgtcaattga gtgataagtg aatcaatcaa ttcccagttg
    37681 atgacgatct gtttttcgtt tttgccaata taagctagat tcacaaatac ttcgattcga
    37741 ccgcggctta actgctgttt aataagctga cgaaaatcgt tttcaacaaa gttcaggatt
    37801 ttaggacttc gtatttgaac atctagaaaa cgctggttga cactttttat ttcaatatct
    37861 aactgatacg cttttgtttc gcggatggct ttgccaaaac cagtcatact tttcattcga
    37921 cagggtcctt cctcattaca tattttctat tgctttttgc agttgttcaa actcttcgtt
    37981 cgtcaatgtc aggcctttcc ccattttttc atggtctggc gcccaatctc gaagatcgaa
    38041 ttttggcggg cgaccattcc agctgactag gttcagttct ttacgccacc ccttgttgtt
    38101 ttctgacaat atagcgattt cttctacgat ttcatatgaa aattcttgtg ccatggttgt
    38161 tcctccttga gataatataa atataggatc agtaatgtgt caactggttg cttaagcaat
    38221 tcatgctgca aacgatcctt gcgttttcct tgtagcagat aatcaaaata aacagttttc
    38281 gtgcgctcat tttgtatttt ttgctggtat tttcgatacc ttcgccattc ttcagtactt
    38341 aattgaccaa tttgtgccaa taaatcatcc ggcatacttt ttatatcttg ttggcagatc
    38401 atttgaatca tcatgtctac ttgatagatt tcattgggca gtaaaaaatg tttttttgct
    38461 aaaagttcta gcatcgccaa tccgcatgtc tctggtacga ccctgttctt atataaatta
    38521 cgcagttttt gccaaaattt tatgcttttg aaaaaagata attctaactg aatttctttt
    38581 gttaagtaag acaaaaatcc tcgccagtat gttcttgcag gtttaacaga atgaaatgta
    38641 taccagttcc catagtaata gcctaatcgt tttttcattt ccagatagcc taattgtaaa
    38701 tttaattggt tcctttggct atcaaaaagc agaaagctgc ctagtgtcca caatgtttga
    38761 catttccaat ggataaaaat gtctggcata cgaatttttt tcgctgggcc tggcccttgc
    38821 acatctacga caaatgcctc tgttgctcct ttgttaatcg caatatcaat aggtattttg
    38881 ttgcgatacc caccgtctgc atatttattt tttccgatct ttcgataagc catagcggga
    38941 taaaatgaag cagaggcgag tatccaatct ggtatttctt ctgtttttgt ctgttggata
    39001 tcagtcacta cttccgctag ttctggcagt ttagtagaca ccgtatataa acgtattttg
    39061 ggattatgtt tgagtaagga caaatccaaa ttgtctttga ccatcttttc caatggagca
    39121 gaagaaaccc cgccttcaat tagtgctgtt ttcgtcattt ggcgtctttc tcgatagaag
    39181 cgttcacgaa ggtcttcaac tgcagccatt tctggtaacg caagcacttg tcttgtcgac
    39241 agctttttcc ataaattcac agcttttttc atatctcctt gtaaaatcaa tgcgccgttc
    39301 aacgcaccta ccgaggtacc tgtgataatc tcaaagttga tatcatgttc cttcagcgcc
    39361 tgccacacac caatctgata agcaccgtgc gctcctcctc cacctaaaac aagtgctgta
    39421 tgatagacca gctttttatt taattgtgtg gttccttgat agccgttttt atgaaaaata
    39481 cgcaaccatt cttctgatag aggagattga aataaaaaac aacagttttc tttaataaaa
    39541 aaacgagcgc aagcttccat ataccgaaaa aattttttcc attcaaaagt ttcaccttca
    39601 atcaacagat tattaaaaat aacatctcct ctacttttag caatagtaaa atagacggta
    39661 tattttttcg atactgcttt aaaagcacat tgcttttttg cagaacgcca aaaagcgtct
    39721 ctactttctt gatgactttg aaaaaatgga aggggttgtt ttgcttgctg gataggcgaa
    39781 tacagcgaca ttttctgtga aaagtaaact ttttttatct ccatcgcgct ctcctttttc
    39841 ttcaagtata ccagtacgtc ataaaaaggt catgagtctc gtattcgact ttgtagattc
    39901 cataagaaaa caattcataa aacctccttt aaataaaaaa atacaaaaat cacaaaaatt
    39961 aattgaaaat gagagtgttt tcaatgaaaa cgtgattttg taatggaatt ttaaaataaa
    40021 agaaacaagc ttttttaatg caaatgttgt aaaataaaag agattaagag gaaataagag
    40081 gaggaacatg atgactgagt cagtttatgt acatattgat accacaagta atgctgtttt
    40141 aacaaaaggg ctgactgcta ctgactttgc aaacagtatc gtgcattttc ctcaaaactt
    40201 gctcttgctt gatccatctg cttctgctgg agaatatgaa tcccatacag ggctgaaagt
    40261 aatccgtggt acagaaaata ttcagcgttt cttttcctct tctcgtaaca ggcgtggatt
    40321 cgatgaattg aagtggatcg actttactga tttaacgatg ctgaaagaat tgacaccttt
    40381 agaaatttca gaattattat attttggtca tatgaaaaca catcttcatt cgccgttttt
    40441 ttataaacta cagaataatt ttgtatactt cgatcttaat gatcagctga atcggattta
    40501 ttatcgctat ttagatgaat tttataggat ttttgctagt aaattaagtt cgatcttgtc
    40561 agataagatc aatgtaaaaa aatctttttt caaaaaagaa acacctgttg aaaaaatcag
    40621 tgtggaatta ttgaaagaaa tgcgtaattt gatgcaggaa gggatcattt tttcttttga
    40681 tcaagttggt ttgatcgatg gtaaatatcg tatcccaatc tatgtgattg aagattatat
    40741 ttggaaatta aaaggcaccc ggtatcgaga agaggaaatc atcgctactt taatctactc
    40801 tgtcaccgat cgacactggc aattggtaga agatgccgat ctttcttatc tgccattgaa
    40861 gtagatctat ttcttatcaa aaaataaaaa ggaaacaaga aactaggaca tgattctgtc
    40921 tcaagtttct tgcttctttt ttatttacag ctcctgtcgt ttatctggca caaaaatcga
    40981 aggagtcgtt tgttggatcc ctaataaaac gatgatcgtt acaatagctg tactcaaccc
    41041 gctggcacca ttcattacga aagaaaagag ccaaggattc atcccccata gtgcatacgc
    41101 tccccagaaa accactcctg caataaaatg ccaaaaatat ctggaaagtg cgccaatcaa
    41161 tgcaccaaac acgatgcttt ttctaatttg tttcgtatta tcgtctacat agcctttttg
    41221 gacatttgaa gccatgagcc ctgctgcgcc tgcaaaagta taggcaatcg ggtactcaat
    41281 caacacttga acgacactta aataataaac ttgtcccaac ggaaaatgta ggatgcccca
    41341 aatgaaacct gccagcatcc ctgcctttgt ccctcttctt aacgcaaaaa gagtcaatgg
    41401 aatctgccct aatgaaatag aaaaactgct gccgatctgg atcggaatca acgacaacac
    41461 catcgctaac gctgcaacaa tcgttccttc tacccaaact ctattttttc ccataaaaaa
    41521 acttcctcct ttgaataaaa aagtcacaaa ggaggaagca gctttcgaaa atagtcaaac
    41581 agctgtttat tcacaattcc tacgctcgca taaccgatac aggtacgaag ggtttagaaa
    41641 tagattcaat ctcagcccaa ttggactccc ctttgtgagt ttatttatta tttatttttt
    41701 gtttcaaaca atggactaac tggtttgttt tcatggatac gtttgattgc atctcccatt
    41761 agtccgccaa cacttacttc atcgattttg tcgattttac gatcttctgg aagatagatc
    41821 gagtctgtaa caactagacg ctcgatagct gaatcttcta ttctttgtaa agcagggccg
    41881 gataatactg gatgtgtaca tgaagcatac acactagttg caccagcttc tttcaaagca
    41941 ttggcagcca atgtgatcgt acctgctgta tcgatcatat catcgatcag tacacaagtt
    42001 ttgcctttga cttgtccaat gatattcatt acttctgcaa catttgcttt tggacggcgt
    42061 ttgtctatga tcgcaatcgg agatttcaaa aattccgcta atttacgtgc ccgtgtgaca
    42121 ccaccatggt ctggtgaaac aacgacaaca tcatcgcctt tgattccttt ttctaagaaa
    42181 taattggcaa taagtggcgc acccatcaaa tgatcgactg gaatatcaaa gaaaccttgg
    42241 atttggactg catgaaggtc caaagtcagc attcttgttg caccagcttt ttgaatcatg
    42301 tttgctacta atttcgctgt gattggttcc cgtgcacgtg ctttacggtc ttgacgcgca
    42361 tagccataat aaggcatgac tacgttgatt gtttttgcac ttgctcgttt cagtgcgtca
    42421 atcatgatca gcaactccat caagttatca ttgactgggc tgctcgttga ttgaataaca
    42481 tagacatgcg atccacgaat actttcttca atgtttactt gaatttctcc atcactaaat
    42541 tgagtaacag atgattttcc taactcgacg ccgactgctg ctgcaatttt ctctgctaat
    42601 gggcgattgg aatttaacgc aaaaattttt aatcttgggt caaagtaatg ttttgacatg
    42661 gggacctcca ctttcttttt cacaactcta gaattctacc taataaatag tagtcatatt
    42721 ttttaaataa atcaagtgat tttgcaaaag tcttagttta gataagggag tttttttgca
    42781 tatccttctt tattgacttg tcttgctctc gcaattgcca gcgcatattc tggaatattg
    42841 tctgtaattg ttgacccggc agcaatcgaa ctgtttttag ctacctcgac aggtccgatg
    42901 atattcgtgc tagaaccaat aaagctgtgg tcgccgacag tcgtatgatg tttgttcttt
    42961 ccatcatagt tgacgaagac aacaccacat ccgacattga tttcttctcc cagcgtagca
    43021 tcaccaacat aagtcaagtg accgacttta gttcctttgc cgatttgagc atttttcact
    43081 tccacaaagt ttccgatatg gacgttttcg ccaatttcag cttttggtcg taaatgtgca
    43141 taaggtccca catctgcatg tttcttgaca tggctgcttt caatgactga gttttcaatg
    43201 acgacgtgat cctcaatcct actgtctacg atttttgaat gtgacccgat gacacaatca
    43261 gaaccaatga ctgtatttcc ttggatttgt acacctgctt cgatcaatgt gtctggaccg
    43321 atttctacgc ctgcatcgat gtatgtcgtt gctgaatcga caaagcttac gccattgacc
    43381 atatgcatct ggttaattcg ctttctcatg atctcatttg ctttagctaa cgcgatccga
    43441 tcattgactc ccattgattc ttcaaaatca tccgtttgat aagcagcaac tgtatggcct
    43501 tcttctttta agatttcgat gatatctgtt agatagtatt caccttgagc attgtttgtt
    43561 ccaactttgc ttaacgcatc gaacaaagct tcgttgtcaa aacagtaagt tcctgtattg
    43621 atttcttgta caagagcttc ttctggtgtt gcatcttttt gttccacgat tttttctacg
    43681 atcccaatat ggtcacggat gattcgtcca tatcctgttg ggttttctgc ttgtgccgtc
    43741 aagatcgttg cacttgcatt ttttccttgg tgatattcaa aaagattgtt cagcgtttca
    43801 gtagtcaata atggtgtatc gccactgata actaacgttg ttcctttttt tccttccaag
    43861 aaagaagcag cttgcaaaac agcatgtcct gttcccaatt gttctgcttg taacgcatat
    43921 ttactgcgtt ctcctagttg tgctttaact tgttctgctc cgtgtccaac gatcgtaatc
    43981 acttcatctg gtttcgtttc actgacccga ttaatgatat gttctaccat aggtttacca
    44041 gaaacgggat gtaaaacttt atataatttt gacttcatgc gggtaccttt ccctgccgcc
    44101 aaaataattg cataacgtgc gtccaactgc gtcactccta aatatagttt gctgatttca
    44161 gtttatccca gtatacccat tttttcaaca aaacaagtga ttttctttta tcccagcgtt
    44221 ttctggataa aaaaatagat agaccataac gggcctatct atttggtaac cggtgctctc
    44281 ctgccaactc ctcggtcaca agtcacacta ctcataaaac atgaaaagct gttttttgaa
    44341 atcgtttctt gtgacttaga gttaaacggt aggcttacaa cctcttcatt aaacgttttt
    44401 caatcagctg accttcggta ttagctcaat aaacggcaaa atatgaggag caatttttcc
    44461 gtttattgag ctaatactca ggacaggacg ctgatttcac aacctctaga taaacggtgt
    44521 tcaaaagcca gtttctcggt attaagccaa atttatgaaa aaatgtggaa agccattttc
    44581 acaaaatttt tcttactact cgaaactaag cggctttttc acaacctctt attctgaaaa
    44641 ataatttcct ggcacaaccg taatattttt cgtttgagta tctacatctt tgacataaag
    44701 cagtgacgta taatcatcga tggcgcgatg accgttgaat tttgattcgg caaatacagt
    44761 gatgccaaca agttctgatt cgaattcttc aatcaagctc ttcattccgt tgactgttcc
    44821 gccgcctttc atgaaatcat ccactacaag gacacgtgaa ccacgtttca aacttctctt
    44881 agacagttcc atcttttcaa ttctttcaga agatccagac acatagttga cacttactgt
    44941 tgatccttct gttatttttg agtcccggcg tacaataacg aatggcgcat tcagataata
    45001 ggacacagct tgggcaatag gtacgccttt tgtggctaca gtcattacag catcgatttt
    45061 ttcaccaagg tatttagaag caatgattct tccaacttga cgcagcagtt caggttctcc
    45121 taacaaatca gagagataaa catatcctcc tggaagcaga cggtcttgtt ctgatagacg
    45181 ttctgctaga gattcaatat attttttcgc ctcttcatat gaaatttcag ggatgaacaa
    45241 tacaccacct gcagcacccg ggattgtttc caaggttcca taccctcgtt ctttaaatgt
    45301 tttcttgatg attcccaaat cttcactgat agaagattta gcagattcat aacgttcggc
    45361 aaagcttgtt aaggaaatca attcatgcgg gtgattcaat aaatactgtg tcatgtcaac
    45421 tagacgttca ctgcgacgta ctttcaccta ttccaactcc ttttaagaaa ttttatcgtt
    45481 ttacattata taagtttttc ttcaaaaatt cgagcatttt atga
//
LOCUS       DARBEC010000012        44875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.012, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000012 DARBEC010000000
VERSION     DARBEC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.012"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            212..604
                     /locus_tag="SCL80_000633"
     CDS             212..604
                     /locus_tag="SCL80_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1106525.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="SCL80_000634"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="SCL80_000634"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEG1106526.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3165..4775
                     /locus_tag="SCL80_000635"
     CDS             3165..4775
                     /locus_tag="SCL80_000635"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEG1106527.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5067..5936
                     /locus_tag="SCL80_000636"
     CDS             5067..5936
                     /locus_tag="SCL80_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEG1106528.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6101..7372
                     /locus_tag="SCL80_000637"
     CDS             6101..7372
                     /locus_tag="SCL80_000637"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1106529.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7369..8664
                     /gene="rho"
                     /locus_tag="SCL80_000638"
     CDS             7369..8664
                     /gene="rho"
                     /locus_tag="SCL80_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEG1106530.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8763..9026
                     /locus_tag="SCL80_000639"
     CDS             8763..9026
                     /locus_tag="SCL80_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEG1106531.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9471..9920
                     /locus_tag="SCL80_000640"
     CDS             9471..9920
                     /locus_tag="SCL80_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1106532.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9977..11395
                     /locus_tag="SCL80_000641"
     CDS             9977..11395
                     /locus_tag="SCL80_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1106533.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11395..12255
                     /locus_tag="SCL80_000642"
     CDS             11395..12255
                     /locus_tag="SCL80_000642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEG1106534.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12429..13202
                     /locus_tag="SCL80_000643"
     CDS             12429..13202
                     /locus_tag="SCL80_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1106535.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13349..13714
                     /locus_tag="SCL80_000644"
     CDS             13349..13714
                     /locus_tag="SCL80_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEG1106536.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13862..15070)
                     /locus_tag="SCL80_000645"
     CDS             complement(13862..15070)
                     /locus_tag="SCL80_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1106537.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15089..15367)
                     /locus_tag="SCL80_000646"
     CDS             complement(15089..15367)
                     /locus_tag="SCL80_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106538.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15521..16216
                     /locus_tag="SCL80_000647"
     CDS             15521..16216
                     /locus_tag="SCL80_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1106539.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16658..17455)
                     /locus_tag="SCL80_000648"
     CDS             complement(16658..17455)
                     /locus_tag="SCL80_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEG1106540.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17452..18636)
                     /locus_tag="SCL80_000649"
     CDS             complement(17452..18636)
                     /locus_tag="SCL80_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1106541.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18661..19908)
                     /locus_tag="SCL80_000650"
     CDS             complement(18661..19908)
                     /locus_tag="SCL80_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEG1106542.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20123..21868
                     /gene="dnaX"
                     /locus_tag="SCL80_000651"
     CDS             20123..21868
                     /gene="dnaX"
                     /locus_tag="SCL80_000651"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEG1106543.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21888..22202
                     /locus_tag="SCL80_000652"
     CDS             21888..22202
                     /locus_tag="SCL80_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEG1106544.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22227..22823
                     /gene="recR"
                     /locus_tag="SCL80_000653"
     CDS             22227..22823
                     /gene="recR"
                     /locus_tag="SCL80_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEG1106545.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22887..23531
                     /locus_tag="SCL80_000654"
     CDS             22887..23531
                     /locus_tag="SCL80_000654"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEG1106546.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23546..23875
                     /locus_tag="SCL80_000655"
     CDS             23546..23875
                     /locus_tag="SCL80_000655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEG1106547.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23889..24827
                     /gene="holB"
                     /locus_tag="SCL80_000656"
     CDS             23889..24827
                     /gene="holB"
                     /locus_tag="SCL80_000656"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEG1106548.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24863..25687
                     /locus_tag="SCL80_000657"
     CDS             24863..25687
                     /locus_tag="SCL80_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEG1106549.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25680..26027
                     /locus_tag="SCL80_000658"
     CDS             25680..26027
                     /locus_tag="SCL80_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEG1106550.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26096..26968
                     /gene="rsmI"
                     /locus_tag="SCL80_000659"
     CDS             26096..26968
                     /gene="rsmI"
                     /locus_tag="SCL80_000659"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEG1106551.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27077..28198
                     /gene="dinB"
                     /locus_tag="SCL80_000660"
     CDS             27077..28198
                     /gene="dinB"
                     /locus_tag="SCL80_000660"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEG1106552.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCL80_000661"
     CDS             complement(28252..28854)
                     /gene="nrdG"
                     /locus_tag="SCL80_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEG1106553.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYAFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCL80_000662"
     CDS             complement(29169..31358)
                     /gene="nrdD"
                     /locus_tag="SCL80_000662"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEG1106554.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGSFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31657..32451
                     /locus_tag="SCL80_000663"
     CDS             31657..32451
                     /locus_tag="SCL80_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106555.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32441..34483
                     /locus_tag="SCL80_000664"
     CDS             32441..34483
                     /locus_tag="SCL80_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106556.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34764..34913
                     /locus_tag="SCL80_000665"
     CDS             34764..34913
                     /locus_tag="SCL80_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEG1106557.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34924..36438
                     /gene="dltA"
                     /locus_tag="SCL80_000666"
     CDS             34924..36438
                     /gene="dltA"
                     /locus_tag="SCL80_000666"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEG1106558.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36435..37640
                     /gene="dltB"
                     /locus_tag="SCL80_000667"
     CDS             36435..37640
                     /gene="dltB"
                     /locus_tag="SCL80_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEG1106559.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37685..37918
                     /gene="dltC"
                     /locus_tag="SCL80_000668"
     CDS             37685..37918
                     /gene="dltC"
                     /locus_tag="SCL80_000668"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEG1106560.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWSTPNKIIQQATALKG"
     gene            37921..39189
                     /gene="dltD"
                     /locus_tag="SCL80_000669"
     CDS             37921..39189
                     /gene="dltD"
                     /locus_tag="SCL80_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEG1106561.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39330..40412
                     /locus_tag="SCL80_000670"
     CDS             39330..40412
                     /locus_tag="SCL80_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEG1106562.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40412..41215
                     /locus_tag="SCL80_000671"
     CDS             40412..41215
                     /locus_tag="SCL80_000671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1106563.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41285..42025
                     /locus_tag="SCL80_000672"
     CDS             41285..42025
                     /locus_tag="SCL80_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1106564.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42036..43040
                     /locus_tag="SCL80_000673"
     CDS             42036..43040
                     /locus_tag="SCL80_000673"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEG1106565.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43085..44782)
                     /locus_tag="SCL80_000674"
     CDS             complement(43085..44782)
                     /locus_tag="SCL80_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106566.1"
                     /translation="MKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLE
                     KPLDEANLLIKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTL
                     TVSSPYQNYAGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMY
                     KQKRLEYSLILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYY
                     WSRMFYYKKWYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLAC
                     IGEAYKNNRFYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISY
                     NLIDHILNTDKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLM
                     HTDQTKAKNYVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLP
                     EELPINDDDLAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYD
                     TNLFSRGITEELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
BASE COUNT        14425 a         7831 c         9343 g        13276 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacctg agtattagag aaactaacgg
     2041 aaaaatagtt cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa
     2101 caccgtttag tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa
     2161 gcaaaaacag cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt
     2221 tgaacaccgt ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat
     2281 tttgtgaaaa tggctttcta catttttgca taaatttggc ttaataccga gaaactggct
     2341 tttgaatacc gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat
     2401 aatttcaaaa acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc
     2461 aggagaacac cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt
     2521 ttacttatgt gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag
     2581 gtcatctttg aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat
     2641 atgccaaaaa tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg
     2701 aaagaaaaag attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt
     2761 agttctttgt taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta
     2821 tttcacttaa aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg
     2881 tttatatcaa gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa
     2941 taatttctca aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta
     3001 cttgactttc ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga
     3061 agtttacaga aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc
     3121 tgagactttt cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt
     3181 ttgtgaccgg cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac
     3241 gcttattgaa aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg
     3301 tggatccggg gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg
     3361 ctgaaacaga tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat
     3421 actccaacgt gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg
     3481 aatacttggg agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa
     3541 tcatgcgagc agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag
     3601 taggggatat agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg
     3661 gtacagataa tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag
     3721 aaatgaaaac taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac
     3781 cgaatatttt ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg
     3841 cacaattttg tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt
     3901 actccatccc attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa
     3961 aactagatac accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga
     4021 acttgaagaa aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat
     4081 atatctcagt agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga
     4141 tcgattggat caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg
     4201 cagatggaat cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag
     4261 cgattcgtta tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga
     4321 tggcttgtgt cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa
     4381 cgaatccaga tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga
     4441 atttgggtgg aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga
     4501 cggctgaagc atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt
     4561 tcaataacaa atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac
     4621 cagataaccg tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc
     4681 aattccaccc agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg
     4741 tcggagcatc attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa
     4801 agtagcaaga gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta
     4861 ttatttgtag gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat
     4921 attttatttt ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa
     4981 ctaccgaaat gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt
     5041 ttacaaaact taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag
     5101 ctgctcgtaa aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc
     5161 aagcaatcct tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg
     5221 gtgcagctaa atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg
     5281 aagcaatgaa catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg
     5341 ctttggaatg tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat
     5401 ttgaagaaaa cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct
     5461 cagttgaatg tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg
     5521 aattagctga tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt
     5581 gcggtatcgg taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc
     5641 acttacaagc aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat
     5701 caggtattcc tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg
     5761 ttaacactga attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta
     5821 aagataaaga aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa
     5881 tcgttgaaga tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat
     5941 ccacataaca gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct
     6001 tttttactta aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag
     6061 catatccatg aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa
     6121 aggaaaccga cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc
     6181 attgattcca gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat
     6241 acaagatgtt cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc
     6301 caacaacata ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa
     6361 aatcaatagt ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga
     6421 agcggtagta ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt
     6481 caaaggattt gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg
     6541 gacagaaaat aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc
     6601 aacgatcaat gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc
     6661 tgcacgagaa ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt
     6721 tcgaggagcg ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg
     6781 gcatttcatg attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt
     6841 aggaaatggt atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa
     6901 attgcaggaa attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc
     6961 gaaagaatta aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct
     7021 gcagcagcca cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac
     7081 gatctatgcg aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac
     7141 cgtggaagga aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt
     7201 ggcttctgat cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac
     7261 aacgacgatt tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact
     7321 aacagcctta ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat
     7381 ttgacaatgg cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa
     7441 tttaaaattc cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt
     7501 gcacaagcag agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa
     7561 gatggttacg gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt
     7621 tctgcctcac aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc
     7681 cgtccaccta aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga
     7741 aaagatccgg aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg
     7801 caaagacagc ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc
     7861 ttttctccaa tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa
     7921 acttctgtat taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta
     7981 atcgttctac tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa
     8041 ggcgacgtgg tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa
     8101 ttagtattgg aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg
     8161 gatagtatta ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca
     8221 ttgagtggcg ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca
     8281 agaaacatcg aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga
     8341 agccgtatgg atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa
     8401 ctttcacgtg aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt
     8461 acccgaaaag aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga
     8521 aaaaatatga ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa
     8581 acaaaaaaca atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat
     8641 ccgaccagaa atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg
     8701 ttgtaaatcg acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa
     8761 tcatgaaaga aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg
     8821 gtttcaaatt cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg
     8881 gtaatacata cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg
     8941 gacgtcaaaa attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc
     9001 tcaaagacgc aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta
     9061 tctgataggg atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct
     9121 gaaaaagtcg taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta
     9181 tccacttgat aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa
     9241 accatttacc taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta
     9301 tagacttgcg attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga
     9361 tgggtctttt tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg
     9421 acctcagttc caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg
     9481 atcaattgat cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat
     9541 tatttgagcg aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag
     9601 tcgttaaggc actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg
     9661 ggattcccca tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag
     9721 ccaaattaac ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg
     9781 tagttcctga aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat
     9841 tgatgaatgc agattttaga aagggaataa aagaagccac tacgccagca gatatcatca
     9901 gacagattag tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa
     9961 aaggaggaaa ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca
    10021 tacttatatg tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa
    10081 attcgaaaca caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc
    10141 agatgcaatc attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa
    10201 agtaaagcgt gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc
    10261 attgagagat attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa
    10321 aaagccgaca atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat
    10381 cgcaggcgga atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa
    10441 ctcagcagac tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt
    10501 tgccatgatg gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg
    10561 ttttgcccca ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg
    10621 cggaatgatt ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag
    10681 cgtaaaaaaa gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt
    10741 aattattggg cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc
    10801 tttgacaaat tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat
    10861 cgggatcatg atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc
    10921 gatcggtttg atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat
    10981 gacacctccc ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga
    11041 agaacggact tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc
    11101 tataccgttt gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc
    11161 agtcgctgca gggatttcca tggcagtagg agttacttca atggcacctc atggagggat
    11221 ttgggtgatg ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc
    11281 atcaggtaca gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa
    11341 aaaacaagac aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta
    11401 tatacaatga aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc
    11461 aatatttgca gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca
    11521 ccagtaatct tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg
    11581 gcagtaagag cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc
    11641 ggtacgatag aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat
    11701 gcgtctttga aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct
    11761 catgcggtaa atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct
    11821 gaaggaggtg ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa
    11881 gaaacaggaa tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa
    11941 aacagaactc caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt
    12001 ccatttgttt tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa
    12061 tacggagttg ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg
    12121 agagaaatat tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct
    12181 aataaaaagg tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag
    12241 gctagtttat actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat
    12301 accctcttaa ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc
    12361 tcgaagccaa tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg
    12421 gtgattttat gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa
    12481 acaaccaatt tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga
    12541 ttaaacgtga ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg
    12601 ccatgcttat tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag
    12661 aagaagaaac agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata
    12721 tggtcttatt cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat
    12781 ttgatggttt gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata
    12841 caacggcaaa agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg
    12901 gttcaaaagc ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc
    12961 gcgggctaga tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa
    13021 aagcttttag aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac
    13081 ataaattcat gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag
    13141 aactgacaaa agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat
    13201 aaattattac gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc
    13261 tcgcttctct cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt
    13321 ttttatccag gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc
    13381 aatcatgatc tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat
    13441 ggattcaacg attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt
    13501 gatcttacgt aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt
    13561 tatcttgtgg ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt
    13621 gattcctgct ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca
    13681 aggcatcgta ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga
    13741 taaatgaaag ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta
    13801 ctcaaaaaac cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag
    13861 atcaaatttc atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc
    13921 ggccattaac aagatagagt gctgtacata tcaatggaat gactccggca atcacataca
    13981 atcctctaaa tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc
    14041 ctaggtctaa accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga
    14101 tttttaaaca aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg
    14161 ctccagagat aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg
    14221 ttaagaataa atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc
    14281 cagtcaatgg acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag
    14341 cagcaactaa gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag
    14401 aaagacccat caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat
    14461 tgatattcca tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag
    14521 gaaagacaag gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga
    14581 aacgaaaatc agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta
    14641 aactagtact taatccataa taattgatgc cttccccttt tttagatgca gggatatatg
    14701 cagtaacgat agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc
    14761 gaactaaata caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat
    14821 agaagatcgc gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca
    14881 tgaataagcg tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc
    14941 tcaagctggc gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat
    15001 aaatcaggta gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt
    15061 tcttttccat gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt
    15121 gaacttctac atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat
    15181 ctcttgtaga cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca
    15241 ctgctttgat caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt
    15301 tcatatcaaa tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt
    15361 aattcatgtt ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa
    15421 aaaaggaaaa aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta
    15481 gtaaaatacc tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag
    15541 tttggatggg ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga
    15601 ttttccgatt atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc
    15661 ccatacgtat atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag
    15721 agaatttaac cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata
    15781 ttcacaattc atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta
    15841 tcgaatcaat cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta
    15901 tgtaaaagag tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct
    15961 attaaacgga aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg
    16021 tgtgcggaca gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa
    16081 attttgtggc atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga
    16141 tggtattatc accgttaaag accatcatat cgttattttg aatatggact tgttagaaga
    16201 caatattatc ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag
    16261 agaaacaaac ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt
    16321 cagctgattg aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca
    16381 aggaacaatt tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg
    16441 agttggtagg agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa
    16501 taagaagagc caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt
    16561 agatgtaagc ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt
    16621 cacagccctt tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc
    16681 caaaagtccc gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg
    16741 ggcttttggg gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag
    16801 ttaatttgat actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat
    16861 aacgcgaaac agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa
    16921 tgatgatttc tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta
    16981 cacgataatg tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag
    17041 cttttgcagc attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg
    17101 aatcgattct gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt
    17161 cacgctgtcc catatctaat agcaatactg agccattttg atcatttccg actgcgtctc
    17221 ttaagtaatt atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca
    17281 agactcgtcc tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt
    17341 gatctgttgt gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca
    17401 ccggccagcc aacacttact agcaaaagaa agaataaaaa aaactttttc atcatatcaa
    17461 gtaacctccg atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc
    17521 caattttttc tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc
    17581 gtagtagtag acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag
    17641 taatacgtaa ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt
    17701 tgtatatcca tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg
    17761 aatctgtgat ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa
    17821 atactggctt ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat
    17881 cttttgttgc gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc
    17941 tgctcttgaa acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat
    18001 gcggataact gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata
    18061 agggaaatac acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac
    18121 aaaccatttt ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc
    18181 aaaaggaagt actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg
    18241 attatggcag tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc
    18301 acagttggga caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt
    18361 atctgaaaaa ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag
    18421 ctgttcttgg gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc
    18481 gccagccata cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata
    18541 gatattcagg cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc
    18601 gccgcaatta ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa
    18661 ttaattaacg gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc
    18721 gttggaaaga tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac
    18781 gttttctaca ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc
    18841 gcaaactgga catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc
    18901 tgcttgttgt ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt
    18961 tgcattcatt cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc
    19021 ggctgcttcc ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc
    19081 acctagcatc gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga
    19141 aatgctggct acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc
    19201 caatgcagta tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg
    19261 ttgaccatct gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa
    19321 atactgctca ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt
    19381 ataaaataaa atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa
    19441 ttctgcgtta tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc
    19501 ctttatttct tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa
    19561 tttgaatcgg tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg
    19621 agctgcgtca tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc
    19681 accatcgacc aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa
    19741 aacatcttct gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc
    19801 ttttgtcatg accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc
    19861 tgccagaccg ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc
    19921 ctctgattca gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga
    19981 aacttctcgg actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa
    20041 aaagccgaga aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat
    20101 tttttaaaaa agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca
    20161 gcgtttcgaa gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt
    20221 ttcccacaaa acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag
    20281 tgcagcaaag attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg
    20341 caacgaatgc gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat
    20401 cgatgctgcc agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta
    20461 tgcgcctaca aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac
    20521 aggagctttc aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat
    20581 cttagcaacg accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt
    20641 tgattttaaa cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga
    20701 atcgactatc agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg
    20761 gatgcgggat gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac
    20821 tttagatgat gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt
    20881 gagtgcttgc gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc
    20941 agccggcaaa gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct
    21001 gatgtatcag caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt
    21061 tacagaattg gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag
    21121 cgatactcaa aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac
    21181 agtcaaactt gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga
    21241 tgatcagata gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga
    21301 actacgcagt aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca
    21361 taaaaaacag acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga
    21421 agcgacaaga cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct
    21481 atctgtcact cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg
    21541 actagtgatt gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt
    21601 gcagctttcg gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt
    21661 ttatattaca agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga
    21721 agagggaaca gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc
    21781 tcaagaacaa gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc
    21841 cgaagctgtt acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa
    21901 tgggcaatat gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag
    21961 cacaagagct gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag
    22021 cggtttttac aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc
    22081 ctgatgatat cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac
    22141 aaattgatgt ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt
    22201 agttttaaac tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg
    22261 acagctacat gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata
    22321 cgatcgatat gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac
    22381 gcgatttgca tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat
    22441 gtagaaatcc ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa
    22501 tggcaatgga aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat
    22561 caccgatgga agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc
    22621 atgatgcaga tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa
    22681 cagctatgta tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac
    22741 atggtctgtc tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg
    22801 tcgaaggaag acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa
    22861 gcagcacaag agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg
    22921 atggtgcagg aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa
    22981 aaacagaaat cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag
    23041 cagtgatcct tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg
    23101 cagcagcaag aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa
    23161 tcgttttatg cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca
    23221 tcggcgttac cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt
    23281 ttacccttta tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac
    23341 aaaatcaaat cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg
    23401 cttacttaaa gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa
    23461 gctttgaaga agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt
    23521 ttgaaaacta ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga
    23581 cagcaaccgt ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc
    23641 gacaggcggc ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg
    23701 tgtagaagaa gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc
    23761 tacacctgtt acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga
    23821 agtaggcggt gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga
    23881 gctgagctat gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc
    23941 ttcaacggag ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag
    24001 gaacaggaaa acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga
    24061 aagacaatct gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc
    24121 cagatgtttt ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt
    24181 tgcagacgga attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag
    24241 aagctgaaaa aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc
    24301 ctggagattt tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc
    24361 aatctcgttg tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat
    24421 tgcaagaaga gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt
    24481 taggaaaagc agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt
    24541 atcaatggtt cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa
    24601 aattggtaaa atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt
    24661 tttattatca ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac
    24721 agataaacca gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca
    24781 gttttcaggc tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg
    24841 atgatgatca aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg
    24901 tcatatctat tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt
    24961 cgaatctcag caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc
    25021 caatgacctt cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga
    25081 aaaagacaaa aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt
    25141 gcagttgcgt gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa
    25201 attgattttt tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct
    25261 ggccgctgta tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa
    25321 gataataggc ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga
    25381 tttcgtgcct gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa
    25441 aatttccggt ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga
    25501 gacagcgaaa aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg
    25561 gaaagtcgta gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga
    25621 aaatcctgtc gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa
    25681 tggataagag atcattatac gacggattga atcaattaga atcagagttg agaaatacat
    25741 taacagaact tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt
    25801 tggagatgga aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag
    25861 ctccagctga aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt
    25921 atgaagaagg attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc
    25981 cttgtgcgtt ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag
    26041 gagactggag cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta
    26101 taatcaaaaa agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc
    26161 aattggaaat ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct
    26221 tatcgcaagt gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac
    26281 accccaaaaa agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt
    26341 gttaaaagaa ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga
    26401 tcctggacat gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc
    26461 aggacctaca gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt
    26521 attttatggc ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa
    26581 agaacagaca gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac
    26641 gaatttttta gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt
    26701 ccatgaagaa taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc
    26761 agtaaagggt gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga
    26821 agttgatgaa gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga
    26881 aaaaccaaat gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt
    26941 ctaccgtcag taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa
    27001 tgaggaaatc agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa
    27061 tgaataagag gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa
    27121 aatcattcat attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga
    27181 gtttcgcgga catccattag taattgccag acaccccagt gacactggtg ggcgtggagt
    27241 agtgactaca gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca
    27301 aaaagcgtat gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag
    27361 cgatatttcc aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt
    27421 tagtatcgat gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat
    27481 caaaatcgcc cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc
    27541 gggagtaagt tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg
    27601 aatcacggta gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa
    27661 atttcatggg gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg
    27721 aaaagaccta tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta
    27781 ttctctttat cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg
    27841 aaaatctgtg ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct
    27901 gactcagttg cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca
    27961 tgggaaaaca gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt
    28021 gacattgcca gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg
    28081 ggaggaaatc ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct
    28141 ggacccgatg acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa
    28201 acagctttga gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa
    28261 gtcttgcttt ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac
    28321 aacttgattg gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg
    28381 aaattgtagt tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat
    28441 ttgcgaaagg agttctaatt tatcttctga atcttgtagc aattcatcaa aggcatagcc
    28501 tgtccatgac cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg
    28561 cagacaaaca tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta
    28621 atcgttgcgc aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata
    28681 tcggaaattt tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac
    28741 gtaaagactg ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat
    28801 atagttttgg ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa
    28861 acttctttca tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat
    28921 aaataagaac aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga
    28981 gggcgttcaa agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct
    29041 cttattctga ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg
    29101 aaaaatatga ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt
    29161 tcacaacctc atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc
    29221 atcggacgag cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt
    29281 tttggatcat gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca
    29341 ccttcgaaac cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt
    29401 ttgtcatatg caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg
    29461 tattcacagt agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc
    29521 aatttttcaa atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat
    29581 tctttatctg tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg
    29641 tcagttagac tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc
    29701 catgcatctg cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct
    29761 gggtttgttt cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag
    29821 cctaacgata cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa
    29881 agacgtttcc cgaaagaacc atacatatat aaaattggcg catttgctgg aatggcttct
    29941 ttacaacgtt ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt
    30001 aactgccaga atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat
    30061 gtaacaacac ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac
    30121 ccttgcaaga atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt
    30181 ttatcgtaat tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt
    30241 ttgatgtcgt aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc
    30301 agtttaggga agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa
    30361 attgcttttt gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg
    30421 aaaggagttt gtccatttga agtaaataag gtattgattt catattccaa actttgcata
    30481 gcatcgaaga tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa
    30541 tcgatccatt cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca
    30601 agcaattcat ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca
    30661 ataatctgtg agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca
    30721 ttgccgattt tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc
    30781 atcggtgtat aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca
    30841 tttgctatat gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc
    30901 agatctcgct gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt
    30961 tggtccttgt tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca
    31021 aaatctcgtt tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct
    31081 agtaatatat gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg
    31141 cttttgattt cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt
    31201 gataatgagc cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca
    31261 tcgaaatcaa catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc
    31321 tgagtaacga cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt
    31381 gttcttgtct atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag
    31441 tattcaatgt gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa
    31501 aaaaggtgaa aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa
    31561 aataattgaa taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa
    31621 ttattcgtag atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa
    31681 gaattggaaa agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct
    31741 cccgtcaaag ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt
    31801 atgggcccaa gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg
    31861 cctacgatgg gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag
    31921 ctcagtgact ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat
    31981 accctaaatg caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag
    32041 gaaatggaaa agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa
    32101 cggtatcccg atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt
    32161 gttgttcagc cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg
    32221 gctacagaat tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg
    32281 ttagtcaccc atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat
    32341 ggtgtgattt tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta
    32401 atagacatgc aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt
    32461 taaaagtttt tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc
    32521 cgtaatgatc tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc
    32581 aagtcaagat acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt
    32641 tttgcttttt ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat
    32701 aggcatctat cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga
    32761 aatcatgata attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc
    32821 caaactcttt tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt
    32881 tatctccatt ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt
    32941 atctgtatat tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa
    33001 agaacaagca gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat
    33061 cgggttacta cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc
    33121 atggatgatc agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt
    33181 tttcgtttgt gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat
    33241 catattgagt aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac
    33301 gcaagttcat ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat
    33361 ttctttaatg acaattggcg gcacggctag tatctctact attttatcct accaggagaa
    33421 agtttcctcc ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat
    33481 tctagcagag gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg
    33541 gagtatctat agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga
    33601 aacacaatat caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc
    33661 acctggtcat actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata
    33721 tggatctagt attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag
    33781 tgtattaggt gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt
    33841 ttttgaaaaa gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa
    33901 taatgaaaaa atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt
    33961 ttattattct tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact
    34021 tccggatgac caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc
    34081 ttccagctat tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac
    34141 actatttgtc gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt
    34201 agcagaagca gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca
    34261 aagaaggatc agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat
    34321 attagggatc actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc
    34381 cgattactgg ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta
    34441 tgtcattacg agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa
    34501 gataataaat agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga
    34561 tttttgtcac aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa
    34621 aaagcagtga tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa
    34681 aacggtttct gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg
    34741 aggttatatg aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt
    34801 ggggaaaatt catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc
    34861 tgtatcacta taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag
    34921 agtatggaaa tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat
    34981 cgagttgctt atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac
    35041 gcacttgctt attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc
    35101 aatttggagt ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat
    35161 ttgccgattg aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa
    35221 ccatctatgg tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata
    35281 acaaaaaaag aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc
    35341 gtaaaaggta cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa
    35401 ggcgtacaaa tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt
    35461 gggatcaagc ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta
    35521 ttttctattt atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt
    35581 gttcaagact ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt
    35641 tctactccat catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg
    35701 ccggaattgg acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt
    35761 ctacttgatc gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact
    35821 gtagctattt caagtattca gattgaccag catgtactag atagttatga ccgactaccg
    35881 attggctacg taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca
    35941 aaaggagaaa cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac
    36001 aatcccgaaa agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca
    36061 ggggatgcag gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag
    36121 caggtcaaat tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa
    36181 gacaaccgaa tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag
    36241 ctggttgctt ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa
    36301 tctatcaaag aacaattact agaagttgta atggattata tgattcctca gaagttccat
    36361 tatgtcgatc tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct
    36421 gaggtgaatc ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt
    36481 ttcttttagc cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat
    36541 taccagttta tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt
    36601 attggcaaca gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg
    36661 gctattttgt ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt
    36721 tagcgataat tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat
    36781 cgttacttgg ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg
    36841 aaataagaga tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt
    36901 tctttccaac aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc
    36961 tccatccgcc tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt
    37021 ttgtcggttt tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg
    37081 tagtgggacg cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt
    37141 acgtgtacag tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta
    37201 caagctacat gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga
    37261 atatcaaaga attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg
    37321 tatatatgcg attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa
    37381 tgtcaaatat cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt
    37441 ggtactacat cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga
    37501 tccgatttaa gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag
    37561 ccgtattttt gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag
    37621 ataaactttt ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag
    37681 gaaaatggaa gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag
    37741 aaaaaatcgt gatattgatc tatttgatga agggttgctt gattctttag catctgttca
    37801 attattagtc gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg
    37861 agaggattgg agcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt
    37921 atgaaaaaga aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt
    37981 gtgtttttta cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc
    38041 tcttcttcca tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct
    38101 gaaaaacaat atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat
    38161 ccgtctgttc tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc
    38221 gggacacaat cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac
    38281 aagaaagtcg tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat
    38341 tactttaata cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa
    38401 gtaacgcctg ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag
    38461 aatgatacgt tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa
    38521 tctttgaatc agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc
    38581 aatattgggt tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa
    38641 caataccaag aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg
    38701 aataatccat tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca
    38761 gagctcaaac agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt
    38821 cagctggtct tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg
    38881 gttaacgaga aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc
    38941 aaaaagatca aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat
    39001 caagcgggta ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg
    39061 gctgcagatc aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat
    39121 catcttgacg atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa
    39181 ttaaaataaa gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg
    39241 aataaaagat aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac
    39301 aatatgaata tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg
    39361 atttaacttc tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag
    39421 aatatgccaa accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca
    39481 aacatggtgc tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt
    39541 ttatggtaac aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt
    39601 gggggcaagt catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc
    39661 acggtgtgat tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt
    39721 atgaaataaa ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt
    39781 ggggcatacg tcctggagat atggtcactc catatattga atacaaacga atgaatggca
    39841 gtaaatattt actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag
    39901 tattagagaa tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc
    39961 aagaagaagt aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg
    40021 cttttgcttt agatactggt acagcaggag acactccagg aatgacacca aaagaagcag
    40081 attcgatttt aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata
    40141 aaaaattatt gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata
    40201 cagtgatttc tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc
    40261 cgtctgtagc cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg
    40321 aagatgatta tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg
    40381 aaacagttga aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata
    40441 tcggaaaaaa gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt
    40501 cttatctata atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc
    40561 ggaagaaagc atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc
    40621 attcaactat gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag
    40681 taacctttta ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt
    40741 gatcaattca gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat
    40801 cagcggagtg agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt
    40861 catgccagaa gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt
    40921 ttcagcactc atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt
    40981 cgactatcct gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga
    41041 tagaattggt acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc
    41101 aatcgctttg ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga
    41161 attttatgaa caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt
    41221 aagcagttta ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg
    41281 tacgatgaaa ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac
    41341 aacaggttat ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac
    41401 gctgatcaaa ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca
    41461 ttatcatcat gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc
    41521 taagagaaat ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt
    41581 tgaagaattg acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt
    41641 aaaaattggt ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc
    41701 catgcgtatc gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta
    41761 tttggaaagt ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct
    41821 ttttgaagga aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc
    41881 gaaatcagct aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga
    41941 acaattgaaa aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc
    42001 agttgacaaa gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc
    42061 acgacccaag aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg
    42121 aacctatttt gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata
    42181 cgcttgaaga aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct
    42241 taagtggagg cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc
    42301 ctgatgatgg ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac
    42361 ttcatgaatt gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg
    42421 gcatgaaatt ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa
    42481 caattatgtt cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca
    42541 aaatcgaatc aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc
    42601 ttccagaaga aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa
    42661 ttttcggcgt agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga
    42721 aaaaaatcaa tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt
    42781 ctccagattt caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt
    42841 tgaatgtctc agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag
    42901 gagatatctc tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat
    42961 caaaaatagt cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat
    43021 tgcttacctt actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta
    43081 ttttttatac aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag
    43141 tatctttttc ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta
    43201 agtcaaaacg ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat
    43261 cgtaatgtgc actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca
    43321 aataaatttt tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta
    43381 aagcgtggat caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat
    43441 ctgcttggaa ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga
    43501 taagatttgt cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt
    43561 ctaatatctt ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca
    43621 gattctccat ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc
    43681 gaagctgctc ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc
    43741 tccgatcttt gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta
    43801 gaatgataat atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg
    43861 tttggatgta aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta
    43921 cataaaaacg gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc
    43981 ttataacact agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag
    44041 ttgcataaag cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc
    44101 attttttata gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga
    44161 taataaataa atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa
    44221 tatcttctga tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg
    44281 agtattcaag acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag
    44341 cgataaaaac agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac
    44401 taatcaatgg tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat
    44461 aaggagatga gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca
    44521 cagccaatga gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct
    44581 tttcgcctag atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt
    44641 ccaacggttt ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa
    44701 tcgtacgagt gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc
    44761 acgaatcttt tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga
    44821 aataagcaat tcctgttaaa aataacaatg ataatataat gattatgctc cactt
//
LOCUS       DARBEC010000013        43535 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.013, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000013 DARBEC010000000
VERSION     DARBEC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 43535)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 43535)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..43535
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.013"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(174..2555)
                     /locus_tag="SCL80_000675"
     CDS             complement(174..2555)
                     /locus_tag="SCL80_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1106567.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(2545..3192)
                     /gene="recU"
                     /locus_tag="SCL80_000676"
     CDS             complement(2545..3192)
                     /gene="recU"
                     /locus_tag="SCL80_000676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEG1106568.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            3256..3795
                     /locus_tag="SCL80_000677"
     CDS             3256..3795
                     /locus_tag="SCL80_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEG1106569.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            3885..4304
                     /gene="gpsB"
                     /locus_tag="SCL80_000678"
     CDS             3885..4304
                     /gene="gpsB"
                     /locus_tag="SCL80_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEG1106570.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYEAYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            4326..4693
                     /gene="rnpB"
                     /locus_tag="SCL80_000679"
     ncRNA           4326..4693
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="SCL80_000679"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            4902..6068
                     /locus_tag="SCL80_000680"
     CDS             4902..6068
                     /locus_tag="SCL80_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEG1106571.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            6114..7610
                     /locus_tag="SCL80_000681"
     CDS             6114..7610
                     /locus_tag="SCL80_000681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEG1106572.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            7691..8374
                     /locus_tag="SCL80_000682"
     CDS             7691..8374
                     /locus_tag="SCL80_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1106573.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
     gene            9019..10209
                     /locus_tag="SCL80_000683"
     CDS             9019..10209
                     /locus_tag="SCL80_000683"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEG1106574.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
     gene            10311..11264
                     /locus_tag="SCL80_000684"
     CDS             10311..11264
                     /locus_tag="SCL80_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEG1106575.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            11333..12682
                     /locus_tag="SCL80_000685"
     CDS             11333..12682
                     /locus_tag="SCL80_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1106576.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(12865..13188)
                     /locus_tag="SCL80_000686"
     CDS             complement(12865..13188)
                     /locus_tag="SCL80_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106577.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            13317..14480
                     /locus_tag="SCL80_000687"
     CDS             13317..14480
                     /locus_tag="SCL80_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1106578.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            14550..15899
                     /locus_tag="SCL80_000688"
     CDS             14550..15899
                     /locus_tag="SCL80_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1106579.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(15944..16375)
                     /locus_tag="SCL80_000689"
     CDS             complement(15944..16375)
                     /locus_tag="SCL80_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106580.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            16763..19405
                     /gene="alaS"
                     /locus_tag="SCL80_000690"
     CDS             16763..19405
                     /gene="alaS"
                     /locus_tag="SCL80_000690"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEG1106581.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            19605..20201
                     /locus_tag="SCL80_000691"
     CDS             19605..20201
                     /locus_tag="SCL80_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1106582.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            20323..21471
                     /locus_tag="SCL80_000692"
     CDS             20323..21471
                     /locus_tag="SCL80_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1106583.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(21509..21922)
                     /locus_tag="SCL80_000693"
     CDS             complement(21509..21922)
                     /locus_tag="SCL80_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEG1106584.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGADNLARQALHKQLKEKKTL"
     gene            22001..22432
                     /locus_tag="SCL80_000694"
     CDS             22001..22432
                     /locus_tag="SCL80_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEG1106585.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            complement(22531..22764)
                     /locus_tag="SCL80_000695"
     CDS             complement(22531..22764)
                     /locus_tag="SCL80_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEG1106586.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            22947..24617
                     /locus_tag="SCL80_000696"
     CDS             22947..24617
                     /locus_tag="SCL80_000696"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEG1106587.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            24764..25150
                     /locus_tag="SCL80_000697"
     CDS             24764..25150
                     /locus_tag="SCL80_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106588.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            25137..25835
                     /locus_tag="SCL80_000698"
     CDS             25137..25835
                     /locus_tag="SCL80_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1106589.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            25840..26304
                     /locus_tag="SCL80_000699"
     CDS             25840..26304
                     /locus_tag="SCL80_000699"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEG1106590.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            26301..27212
                     /locus_tag="SCL80_000700"
     CDS             26301..27212
                     /locus_tag="SCL80_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1106591.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            27447..27986
                     /gene="pyrR"
                     /locus_tag="SCL80_000701"
     CDS             27447..27986
                     /gene="pyrR"
                     /locus_tag="SCL80_000701"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEG1106592.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            28000..29283
                     /locus_tag="SCL80_000702"
     CDS             28000..29283
                     /locus_tag="SCL80_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEG1106593.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            29296..30222
                     /locus_tag="SCL80_000703"
     CDS             29296..30222
                     /locus_tag="SCL80_000703"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEG1106594.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            30241..31521
                     /locus_tag="SCL80_000704"
     CDS             30241..31521
                     /locus_tag="SCL80_000704"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEG1106595.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            31526..32602
                     /gene="carA"
                     /locus_tag="SCL80_000705"
     CDS             31526..32602
                     /gene="carA"
                     /locus_tag="SCL80_000705"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEG1106596.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            32607..35792
                     /gene="carB"
                     /locus_tag="SCL80_000706"
     CDS             32607..35792
                     /gene="carB"
                     /locus_tag="SCL80_000706"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEG1106597.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            35846..36622
                     /locus_tag="SCL80_000707"
     CDS             35846..36622
                     /locus_tag="SCL80_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEG1106598.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            36619..37551
                     /locus_tag="SCL80_000708"
     CDS             36619..37551
                     /locus_tag="SCL80_000708"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEG1106599.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            37548..38264
                     /gene="pyrF"
                     /locus_tag="SCL80_000709"
     CDS             37548..38264
                     /gene="pyrF"
                     /locus_tag="SCL80_000709"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HEG1106600.1"
                     /translation="MNQLDQRPIIALDFSTRQEVEDFLRFFPKEEKLFVKIGMELFYQ
                     EGPEIVRYLKEAGHNVFLDLKLHDIPNTVEKAMRGLAKLGVDLTCVHAAGGIRMMEAA
                     MRGLEEGTPEGGKRPLLLAITQLTSTSEEEMHADQLIEVSLEKSVIHYASCAKKAGLD
                     GVVSSAWEVEAIKEMAGDEFVCLTPGIRPEGTVAGDQTRVVTPSQAKKIGSTFIVIGR
                     PITQSTNPYKAYQTIQTEWS"
     gene            38271..38960
                     /locus_tag="SCL80_000710"
     CDS             38271..38960
                     /locus_tag="SCL80_000710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HEG1106601.1"
                     /translation="MNVEQSIAKDLLEIEAVFLNPSNPFTWASGIKSPIYCDNRITMS
                     YPKVRKEIAKGLASKIKEAYPEVQVIAGTATAGIPHAAWVAEILDLPMVYIRSKAKDH
                     GKGNQIEGRIFEGQKMVVIEDLISTGGSVLEAAEAAKREGADILGVAAIFTYELPKGK
                     ANFEKAEIPLMTLTNYSVLIEAALEDRYIDEQELTLLKEWKKDPENWQIEARLMKIEK
                     SDLCLQNHQLE"
     gene            38933..39202
                     /locus_tag="SCL80_000711"
     CDS             38933..39202
                     /locus_tag="SCL80_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106602.1"
                     /translation="MSPESSIGIAKIQKLIARSIFSSGMKRNFYRDQFLIFLKFIFLR
                     LVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYLMI"
     gene            39240..39881
                     /locus_tag="SCL80_000712"
     CDS             39240..39881
                     /locus_tag="SCL80_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1106603.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            39924..40616
                     /locus_tag="SCL80_000713"
     CDS             39924..40616
                     /locus_tag="SCL80_000713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEG1106604.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            40613..41515
                     /locus_tag="SCL80_000714"
     CDS             40613..41515
                     /locus_tag="SCL80_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1106605.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(41673..43379)
                     /gene="efbA"
                     /locus_tag="SCL80_000715"
     CDS             complement(41673..43379)
                     /gene="efbA"
                     /locus_tag="SCL80_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEG1106606.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
BASE COUNT        13935 a         7684 c         9467 g        12449 t
ORIGIN      
        1 aaaagaaaaa tggattttct aatttttaag ctaaatgtta gatcacacat gtatatgctg
       61 gatagtctta taaaaaatca tgtaatacag cttggtacat gatttttatt tgtagctgtt
      121 ttttacatag acacaaaaaa gaaaccacgc ttgtagacgt ggtttctttt ttactatctg
      181 tttccagaag ctgaggtgct gctggatggt gtatggtcat ttgcagcatc cgctccgcta
      241 gttgctggct cactggaaga agcaggagga gcagatgaag acggtgtcga gctttcttcg
      301 gatgaagaag aaggaaccgt tgttgaagac gctggctcgc ttgtttctgc tgtcgtgctt
      361 tcttttgagg actcagcagt tgattcgctt tgactagtag agctagagct ttgagtcgtt
      421 gtctctgttg ttgagcttcc aggagtctgg acatagctgg aagatggaat agttgttgat
      481 ggtgtaattg cattcgatct agctgtatat tgatctttgt agtacagttc accgcccaca
      541 cgaattaatc cgcttggcat ctcccagtct gtatttgtca cgttagctga aacatactgc
      601 ataagctcgc gataaacatc agaggctacg tgtgacgatt ctgaagtgac aggtgtcatt
      661 tttttattgt aacctgtcca cacggaaatc gaataatttg gcgtatagcc cgcaaatagg
      721 atatctggat acaccccaga agaaatacca agttttgcat actcgtcatc tgtgtaattg
      781 gatgttccgg tcttaccggc ttgatataac ccagcgattt gcgcattcgt accagttcct
      841 tcagtgatcg tatcttttaa aatgtctgtg atcatataag ctgtttctgg agacatggct
      901 gtttttccat ctggctcata agtttcttct gttccgtctt ggaagacgat tttattaaca
      961 tattgaggtt tgtaataagt ccctccattg gcgaaagccg cataggctgc tgccattttt
     1021 aaggaagaag ctccgtattt tgtcccatct tgttcttccg tattactaga gatcgcattc
     1081 gattgatgga tcgtactgta ttcgatccca agatttttta agaaagaagc cactttatct
     1141 gaaccgactt cattgaataa tttgactgct ggaacattac gggataagta aagtgcttgg
     1201 cgtaaagtaa tggtgcccat atattgatta tcccagtttc ctacaggagt cgaagtccct
     1261 tcatagttat acggtgcgtc agtaatagtt tttccagtag aatatttcaa atattcaaaa
     1321 gctggtccgt aatctgtgac aggtttcatc gtagaaccaa aatcacggga agtattcact
     1381 gctaagttgt tgcctaaagt tacgtcttct gcgatgtgac gtccgccaat ctgggctttt
     1441 actttcccgg tattggtgtc gataagggtg gaggcaactt gcatctcgtc gtctgggtaa
     1501 gagacatact gatccgtgtt gacgatatca tatagtttct tttgggcgtc cagatccaaa
     1561 ttcgtataaa tctctaaacc gtctgtgtag acattcttat ctgttttttc ttgtacttca
     1621 ttgatgactt ctttgacata attatcgacg atcttagtat tgtcatcgga ttgagttagt
     1681 tcttgcagac catccgttac cggtacatta acagcttgat catattctgt ttgagagatt
     1741 ttttcgtttt gaagcatcgt atacaaaaca gtatctcgtc ttttcttcgc ctgatctgga
     1801 taaacataag gatcataggc ggaaggggct tgaggcatcc cagcaagcaa tgctgtttgc
     1861 ggtaaagaaa gttctgataa tttcttacca aagtacatct cagaagctgt ctccatcccg
     1921 tacaagccgt ttgacatata cactttattt acgtagtagg tcaagatctc ttgtttcgat
     1981 tttttttgtt ccaatcttac agccatccac gcttcttggg ccttgcgttt cagagtctga
     2041 tcctcggcac tggtggagaa gaaggaaagt ttgatcaatt gctgtgtcaa agtacttccg
     2101 ccttgcagtc cgccagaggt aaagttagag agggcagaac cgatgatccg aatagggtca
     2161 accccgatgt gtttataaaa acggcgatct tctaccgaca cgatcgcatc ttctaatgtt
     2221 ttcggtaatt cattcgcaga aattttttca cgcttttctg cgcctaaatc ttcgaatagt
     2281 tcgccatctt gtgtatatag tttggaagat acggtagcgt ctaatttttt atccgtcaat
     2341 tcgggcgcat cttttgcgta ataccagaaa agtcctacac ctgctaaaaa gaggatacag
     2401 aaaaatgaga gtattcctaa aaggatcttg ataaataatc cttttttatg tgttccagag
     2461 gactttccag aacctttgcc agaattcttc ttcacactct ttttaggagt aggctgtttc
     2521 tgtcgtctag agcttctagt ttgttcattt gccattcttg ctttctcctt caatagataa
     2581 tgttgataaa tagaagtcaa ccgcgtccaa ataaggaatc cgcggagaaa taccagtttt
     2641 gatctctatt cctagttctt cgatcaaaga taaaggtagt gatttgcgtc ctttttcctt
     2701 ttgggcgtcc cagtaagcaa tcaaatgaac actattcaaa taaaaacagc gcttcaaaga
     2761 cgaaaaccat aacaagacaa agcagacccc attttgttcg aggcatgctt tcatatgttc
     2821 gatttgatgg gcatggaagt ttttaaaggg aaaagatgtt ttgttcttgg tttcttttgc
     2881 ttcaaagtcc aagtaaaagc ctcgatagac gccattatag tctgtagttg aggcttgggt
     2941 aaagtaggct tccttgatca cagcagcact tctcttagga tagtctactt tcacgatttg
     3001 gataggggta ggctttttat ggataaccgc caacccgcgg gaaagataat atttattact
     3061 ttcattgatt gcttcttcaa agcgcatccc ccgattgccg aattcagtga tctgatttct
     3121 tttttcttga attttttcga cgggagaggc attcttataa taaggttggc catttggata
     3181 gcgcaatacc atctttaatc acactcctag gccacattat agcaaaaaag cggatagaaa
     3241 gaaaaggaat ctgagatgga taagtcaaaa gtaatctatg tgtctgggta tcgaagtttc
     3301 gaactcggtg tttttaaaga aaatgatcct aaaatccaag ttgttaaaaa tgttttaaaa
     3361 aaagaactcc atcagttagc agaagaaggg ctggaatggg tacttgtcag tgggaatttg
     3421 ggcgtcgaac tatgggctgt gcaaatagta gcggaattga aagaagaata tccggaacta
     3481 tctcttggta ttttgtatcc tttttctgat tttggtaata actggaatga agggaaccaa
     3541 gaaaaaaaac aattagcaga acaattagca gattatgtcg aagctgttag ccatcaaccc
     3601 taccaatcac ctagtcagct aaagaatcac actcgtttta tacttgagca tacagtgggc
     3661 tgtctgctga tctatgatga agaattccct ggaaaaacga agtatttttt tgaagatgca
     3721 cagaattacc aagcagatca tccctacgaa attcggctga tcagtatgga cgatcttcaa
     3781 aattttagtg aataatcgat attgtgtttg atttatcaac ttttttccta tacaatgaaa
     3841 aaggtagaaa tgttattacg taaatgaaaa ctagaggtgt aaatatggcg aatttggttt
     3901 atagtcctaa agacatttta caacaagaat tcaaaacaaa aatgcgcggc tatgatcccg
     3961 ttgaagttga tgaattctta gacaatatca tcaaagatta tgaagcttac agcaaagaac
     4021 tccttgcttt acaagaagaa aacgatcgat tgagtgcaaa agtagctcaa ttatctaaaa
     4081 ctcaaggagc agctcaaaca cgcgtgcaac aaacagaggc accaaagagc gcagcagtga
     4141 ccaattttga tatcttaaaa cgcttgtcaa atcttgaacg tgaagtattt ggtaaaaagc
     4201 ttgatcaaca agcaagtgca gtcaaaccag cacaacctaa tccaaataac tacacaaatg
     4261 cagacacaag cttagatgat aatgaaaaaa cacgtcaatt ttaatttttg attaatgaat
     4321 ttgcggtttt cgggtaatcg cggtctgact tgatcaggct gaggaaagtc catgctcgca
     4381 caggctgtga tgcctgtagt gttcgtgctt agcgaaacca taagctaagg tactctttgg
     4441 agtaacggca ggaaaacaag ctaaggcttc ggctatgctt aagtatcctt gaaagtgcca
     4501 cagtgacgaa gcggctgggg aaacccatcc ggtggaacgc ggtaaacccc tcgagcgagc
     4561 aacccaaaca atggtagggg cgctttttgt ctggaagaag aacaggacaa aaaggcagta
     4621 taactgcaga tagatggtta ccgctttgtt ttttctccct ggaaaagcaa agtacagaac
     4681 atggcttata gaaaaccgca aacatcaggg aattaggtcg tgacaaaagt aatgcgttat
     4741 aagaaacaag aaaaacattt gaaagctatt tttcaatact tcagatgaat cgttcttatt
     4801 acaaaacaaa cttttgtatc accggttatt cggaatttga gtaaaagtgt ggggtaggac
     4861 tgatgtccca cctccttttt ttataaataa ggagattgtg tatggaaaaa aacaggaagt
     4921 tcaacctcgt cgcaactgcg gcaagcgggc tagaagcact ggttggtaaa gaattaagag
     4981 atcttggaat cgaatgccaa gttgagaacg gacgtgcacg ttttgaagga acaatggaaa
     5041 cgatcgctac agctaattta tggttacgta cagcagatcg agtaaaaatc gtggttgggg
     5101 aattcgatgc gtatacgttt gatgaattgt ttgaaaacgt aaaagcattg ccttgggaag
     5161 attttcttcc actagatgca gcttttcctg tagctggaaa atcgatcaag tcgaaactat
     5221 atagcacacc tgattgccaa gcgatcacaa aaaaagcaat cgttgaacgt ctccgtacct
     5281 attatcaccg tccagcttct gtaccactga cggaaactgg cgcacatttt cagttggaag
     5341 tagctttgct gaaagaccat gtgatggtaa ctctggatac gacaggacca agtttattca
     5401 aacgtggtta tcgcttagaa aaaggtggag caccattgaa agaaaacatg gcagcagcac
     5461 ttattatgtt gacaaattgg cgaaaagacc gtccatttta tgatcctgta tgtggttcag
     5521 gtacgctttg tattgaagca gccttaatag gccataatat cgcaccagga ttcaatcgtg
     5581 agtttgcttg cgaatcttgg gattggttca gtcaagaaat catggaaaat gttcgatcag
     5641 cagccgaaga aaaagcagat tatgatattg aattagatat tactggttca gatatcaatg
     5701 gacggatgat tgaaattgcc aaagccaatg cagaagaaat cggattaggc tcttcgatta
     5761 cctttaagca acaagcagtc aaagacttca aaacggataa agaatacggg gtcatcgtgg
     5821 ctaatccgcc ttacggagaa cgtctaggag aagaagaaac tgttcggaaa ttatataaag
     5881 aaatgggcga tgtattccgt ccattaaaaa catggagtaa atatatcttg actagcgact
     5941 tagcgtttga ggaattttac ggacaaaaag caaccaaaaa aagaaaattg tacaatggtg
     6001 cattgcgtac ggatctattc cagtattggg gaactcgtcc gcctagaaaa aaagagaaag
     6061 acgaataatc ttactaattg attcattcaa ttggatgttt ggaggaatag ggaatgaaag
     6121 aaaaagagtt tttacaagaa gtaaaagaaa tcaatcttct acaacaagct ttaggaatcc
     6181 tcgattggga tacacaaaca ggtatgcctg aaaaagctag tgcataccga agtgaagtag
     6241 acagctatct ttacagcatt tatttttcaa aaaaaatcgg tccaaaaatc aaagaagcca
     6301 ttcgctattt ttctgaacat ccggaagaat tgtcggaagt tggaactgcc gtttttgcca
     6361 aagtaaaaga agagtatgat ttggaacatc gcgtaccgga agatcttatg agaaaatata
     6421 cagctgccgt ttcttcagca cacggacaat ggcaaaaagc tagagaaaca caacaatttt
     6481 ctgattttca acacgcattg aatcaaaata ttgaattaac aaaacagttg ataccttact
     6541 ggagaaaaga tgaaaagacg aattatgatg ttttgctgaa tcaatacgaa cccgggatga
     6601 ctgtcgaaat ccttgataag gtatttgatc agcttaaaga aggaattctt tcgatccgcc
     6661 gtacgctcca agaaaaaggg actgaacctg aaacagactt tttaaatcgg agaatgacga
     6721 aaaaacaaca gaagagattt gtagtgaaag taatcgaaca acttggttat gattttagtc
     6781 gtggtcgttt agatgacact gtccacccat ttatgactgg aattaatccg aatgatgcac
     6841 gaatcacgac acgatggaac gaaacagatt tcaaaatggc tgtttttgga gtgatccacg
     6901 aggctggtca tggtatgtat gaacaagaca tcgatgataa atatgcgtat acaccggttt
     6961 atgaaggtac atcaatgggt atccatgaat cacaatcgtt gtttaacgag atcattattg
     7021 gaagtagtcg cagcttctgg aaaaagcaat atcctttctt tcaagaatgt gcagaaggaa
     7081 cattcgatga tattcctttt gacaaatttt atcgttcatt gaaccataca aaatcaagtt
     7141 tggttcgtat cgaggcagac agtttgactt accctctcca tatcatcatt cgttatgaaa
     7201 tcgaaaaaat gattttcaat gaaggggtag atgttgctga actacctgaa atatggaatc
     7261 aaaaatatga agagtatctg ggaatacgac caacaaatga tgtggaaggc atcctacaag
     7321 acgttcattg gtcaggtgca agctttggtt attttccctc ttatgcatta gggtacatgt
     7381 atgcggcaca attacttcat tctttgcagc aagaaataga tgtggaaaat gttctagcgt
     7441 cagacgatta ttctccaatc aaaaactgga tgtcagagca tatccatcaa tatggtgcat
     7501 ctagaaaacc aaatcaatta atcatggatg caacacatga accgcttaat ccgcaatacc
     7561 tgatcgacta tatgaaagac ctgtatttta atgtatatca ggtaaaataa aatacaattt
     7621 acaaagatag ctgaaattca tctaatctat agatgttttc agctatttac ttaagaaaga
     7681 agtggaacga atggataaat tacaagtgat cgctgattat agtatggacc aattagcgca
     7741 tgatcaaaca ggacatggga gcgatcatac caaaagagtt gtcaagttag ctgaaaaaat
     7801 tttggagacg gaaccgcagg cagaccgctt tgtgacgctt gcagcagctt acctacatga
     7861 tacgattgat gataaagtcg ttaaaaatga gaacgaggca aagcaacagc ttcgtgtatt
     7921 ccttcgctct ttgccaatag tagaggaaca gatctcgatg attttttcca ttatcgaaaa
     7981 tatgtccttt tctaaaaatc tctctgaagc ggccgaacta tctctggaag gtaagatcgt
     8041 tcaagacgct gatcgtatcg aagcattagg agcaatcggc attttgcgta ctgcttattt
     8101 cggcggtgga catgggcacc cgatttttga tcctgaactg tatccgcaaa cattcaagga
     8161 caaagaaaat taccgaaaag gcacgacagt gatcaatcat ttttatgaaa aactgttctt
     8221 gctgtctgat aaaatgaata ctgattacgg atatgaagaa gccaaacgaa gagaaacctt
     8281 catgcgtgaa ttcttagaag aattttttct ggaatggtct attgacgatg aaaattttac
     8341 tccagacaat tggatcaaaa ataacaaaaa atgatggagg ttgtgacaga agtggtcagc
     8401 tccaagatat ttcggatcat tttccgtaaa agttacttct ggaacactgt ttaccaagag
     8461 gtcgtgagcc tgccgttcag ctccgagtca caaggaacaa tttcaaaaaa tagcttctcg
     8521 tgtttgatga atattgtaac ttgtgattcg gaattggcag gagaacaccg tttattcgga
     8581 agaataggac tgtgacaaga cttttgtcac agtccttttt tcgaattcaa caagtaaaac
     8641 ttttaagaaa tattcagaga gtaaaattaa aaattgctga taaacgaaga tggaaatttt
     8701 atgtattttt attggtctat gcaataaata aattaaatat tcagaataca attgatttta
     8761 acgaaagggc ttctatcaat ggttgtgttg tatttatctg cccaggtaga gtaaaataga
     8821 tcattatcta aaaggtaatt gttcatacta tataagtcat ttgaaagtaa aaagatactg
     8881 taaataaaaa atgtggttaa ataattctca taatcagtgt aagcgttatc aaaaatatag
     8941 catacttgct ttgtagatga atgacacgat atatgtttgg tatgttctgc aagacagatg
     9001 aatatggagg tggagacaat gtcttccaaa gaactaacaa tgtatttaga gaaagaattg
     9061 gatcaactga aagaaaaggg gttatacaat acgatcgaca tactagaaag tgaaaatggt
     9121 gcttgtatca tcgtagacgg aaaaaagatg atcaatctag catccaacaa ttatcttgga
     9181 tttgccaatc gcgaagaatt gaaaaaagca tgtattgaag caaccgaaac ttatggtgta
     9241 ggagcaggag cagtccgaac gatcaatggt tcgctgaaaa tccatcagca attagaagaa
     9301 aaaatcgcag aattcaaagg gacagaagca gcaatcgcct ttcaatctgg attcaactgc
     9361 aatatgggcg ctatctctgc agtaatgaca aaagaagatg ctatcttatc ggatgaatta
     9421 aaccatgcat ctattattga cggttgccgc ttatccggag caaaaatcat ccgaattaaa
     9481 catcaagata tgaaagacct tgagaaaaaa gcaaaggaag cgatagaaag taagaaatat
     9541 aaaaaaatca tgtatatcac agatggtgtt ttctccatgg atggagacat tgcacgattg
     9601 ccggaaatta ttccgattgt tgaaaaatat ggactgatca cctacgtcga tgatgcgcac
     9661 ggctcaggcg taactggaaa aggtgcagga accgtcaagc attttggatt aagcgacaaa
     9721 atcgatatgc aaatgggcac gttatcgaaa gctataggtg ttgttggggg ttatgtagct
     9781 ggatcgaaaa cactaatcga ctggttgaaa gctagaagtc ggccattcct attttctact
     9841 tcgctaactc caggtgcagc tgcagctgca ttggcttcga ttactttgat gcaagaacat
     9901 ccggaacttg tcgaaaaagt ttgggaaaat gctaattatt ttaaagaaga attgaaaaaa
     9961 gtaggctata acatcggtat gtctgaaaca cctattactc cagtcatttt gggagacgaa
    10021 aaagtgactc agactttctc taagaagctg atcgaacatg ggatttatgc taaaccaatc
    10081 gtttatccaa ctgtgcctct tgggacaggc aggatcagaa atatgccgac tgcggaacat
    10141 acgagggaaa tgcttgatga agcagtagca gtttatcaaa aaataggaaa agaaatggaa
    10201 attatttaac taaatttagt tttttcgtct atttaaaatt agagaacatg ttcacgcata
    10261 tattgttaat catctgaaga tagtagtaaa aaagatcagg gaggatcatc atgaaaaaag
    10321 tattggtgac aggatgttta ggacaaattg gttcagagtt gatcacacgg ctacgtcaag
    10381 aactaggcag tgaaaatgtc attgcgacag atatacgcat gcctgaagtc attgagaaaa
    10441 acgatatctt tgagatccta gatgtaatgg attacgaaaa aatgcgcagc atagcagaac
    10501 aatatcaagt ggacacattg attcatttgg ctgctctttt gtcagctgtt gcagaagcaa
    10561 aaccacaatt agcctggaat ttgaacgtga ctgggctggt caatgcattg gaagtcgcta
    10621 gagaattcca gctgaaattt tttacgccaa gttcaatcgg cgcgtttggt ccaaatactc
    10681 caaaagatca taccccacaa gatactcttc agcgtcctac aaccatgtat ggggtaacga
    10741 aggtagctgg agaactacta tgtgattatt attatgtgaa atatggcgta gatactcgtg
    10801 gtgtccgttt ccctggattg atttcttatg caaccttgcc aggaggcggt acgacagact
    10861 acgcagtcga tatttattat tcagcaatca aagaaggaaa gtacactagt ttcattgatc
    10921 aaggtacatc aatggatatg atgtatatgc cagatgcgat cgatgccatt ataaaactca
    10981 tgaatgcaga tcctgatcga ttggttcatc gaaatgcctt caatatatcg gcgatgtctt
    11041 ttgacccgga agagatcaaa gcatctattc aaaaaatcat accagatttt gaaatggatt
    11101 atgatgtgga tcctgtacgt cagtcaatag cagaatcttg gccaaattca ctagatgttt
    11161 cttgtgcgca agaggaatgg ggcttttcgc cgaaatacga cttagatgtg atgacaaagg
    11221 atatgttgga aaaactgaaa gaaaaattgg tagagacggt ataaaacaag aaccacatcc
    11281 aaaaagtttt ttatactttt gggattggct atggtatgaa aggggacaag ggatggaaga
    11341 atatcaagcc acgccagtaa aagaagtaaa aatagaaaat gaattaaaac gtacgatggg
    11401 attttttact gctttatcga ctgttatggg cacagtgatc ggtgcagggg tattttttaa
    11461 agccgcaagc gtagcagaag ttacaggatc cgccagtttg catatgtttt cttggtttct
    11521 cggtggtatg atttctgtct gtgcaggact aacaggagct gaactggcag ctgctattcc
    11581 agaaacagga gggatgatta aatatatcga acggatttac ggaaatacag cagctttttt
    11641 attgggctgg gcgcaagtcg tgatttattt tccagcaaat gtagcagcct tgtcgatcat
    11701 tttcggtaca cagttcgtca atctatttgg tctttcccag tcaatgattg ttcccgtagc
    11761 tgttacagct gctgtatcga tcctgttgat caatttttta ggttctaaag ctggaggggc
    11821 ttttcagtcg attacgcttg tctgcaagct tattcctttg tttgtgatcg tcattttcgg
    11881 tttgttccga caagaaggag tagacttcca actgtttccg atccaagcag gagaaaatct
    11941 ttcatttttc tcagccttgg gagcgggctt gctagcaacg atgttcgcat acgacggttg
    12001 gatacacgta ggaaatattt cgggagaatt gaaaaagccg gcgaaagatt tgccaaaagc
    12061 aatttcttta ggaatcatcg gaatcatgat cgtttattta ttagtaaatg cggtgttctt
    12121 aagaaccgca tctatcgacg gagtcgcagg aaacagcaat gcggcaagtg acgttgcaaa
    12181 aatgattttt ggcggcttcg gtggaagact ggtcacagtt ggtatcttga tttctgttta
    12241 tggaaccatc aatggctata cccttactgg aatgcgtctc ccttatgtta tggcaaaaga
    12301 aaacaacctg ccttttagca agctctttgc taaacttcat gataaaacta aagttcctgt
    12361 agccgcaggt atcttagagc tagtcatcgc tattggcatg atgatgatcg gtggttttga
    12421 tacactaacg gatatgctga tctttgtcat ttggattttt tatacgatgg tctttgttgg
    12481 tgtcattctt cttcgtaaaa aagaacctga tttgtttcga ccatacaaag ttccaatgta
    12541 tccgtttatt ccactagtag caatcattgg agggactttt atcgtatcaa gcacgcttat
    12601 cactcagacc tttttagctt cgatggggat agtattgaca cttgctggta tcccgattta
    12661 tctctattta aaacgtagat agctaaaaca ttcgaatttt attagaactg cacttctctg
    12721 ccaaacaaaa tattttattt aaatgaggag tacgtggaag aaatgtcaaa agtactcgtt
    12781 aattttgatc acgtaaatgt cgttttgtct acgatgaaat agcaaagtaa aagacagaaa
    12841 tcgggctcaa ccgatttctg tcttttactt tgatttgcgt aactgaacta ttcggcgtac
    12901 gccgcgataa atatttaata aaaccaagca accagaaatg aaaatgggaa gccaaatcag
    12961 atagacaggg tagccaagca ctttagacaa agcatgtgga gtcattttat ccatttcaag
    13021 aagtgcttgc tgccaagtct gaagattgaa gactagcatg aaacccgaaa taatcaggag
    13081 aataaatcct aaataatgaa ttgtagttct ttttcttaca taagcgatcg tagcataaag
    13141 gaacagtaaa aaaataggta cgccaaaaac aaagtacata ttcagcatct cgacaccccc
    13201 tttgtgttta tcttacttta ctttgaaaga aatgagaaat aatagcctcg ttacttgctt
    13261 gaaacagcaa aggactgcta agataagaga gatacaaaga atcaaggagg agtcatatgg
    13321 caagttatat cgaaccgatt cgttttgcgc tgattgtttt tccattttta gccttagcga
    13381 tctcatctgt tttttttatt tatgaatatc gaaagtatgg aacatttcta gtaacaaggg
    13441 cagttatttt gtattctttc gttttttatt tattgtgtgc gtattttttg gtgatcttac
    13501 cacttccggc aaaggagacg gttgcccaaa tgacagggcc aaagatggag ctgcatttat
    13561 ttgcttcatg gcaacatttc agagacaaca cggtattggt cttgtctgaa cctagtacct
    13621 acctgccggc aatgaagcag agtgtatttt tggaaccagc ttttaatgtt ttattactca
    13681 taccatttgg catttattta agatattatt tccggctttc ttttatgaaa acgatgattg
    13741 ctagtttctg cctttctcta ttttttgaat tgacgcagct aagtggactt tattttatct
    13801 acccaagacc ataccgctta tttgatgtga atgatttagt gacaaataca ttgggtggaa
    13861 tcattggttt tgtcgttacg ccgttattta catttatgct tcctacaaga agacgaatgg
    13921 atgaactttc ttatgaaaaa ggaaagactg tctcattgac tcgacgcttg gtcgcttatt
    13981 tgatcgactg gacattttta tctcttatta atttggtagc ggcgattttc tttaaaattg
    14041 cgttcgattc aggggcagtt acttctcata tctggtggct tttattagaa gtaggggtat
    14101 actttatgat tgtaccgttt ataaccaatg gatggacact tggcaaaaaa attgtgcgta
    14161 tccgtttggt ggaagaagac cgcgaacgaa tttctttaaa agcattgatg atccgctacg
    14221 ggtatctgta ctttcttttt tatggtttaa gctggttggg gcagttgctt cagcaaatca
    14281 tgaattcgac aacgaatacg gtgttgcaga tgagtgcagt attcatttct ctcggtgtct
    14341 tgttactaca aggtattttt attttaaata tcttgctatc atttttgaga aaaaaacgga
    14401 ttctgtttta tgaaaaagct agtcacacat atactgttag cacgattggc caaacaaagg
    14461 atgatgattc tcgaaattag ctgagcaaaa acgctttgtc tgtgttataa tgccaaagtc
    14521 gtgataattt aaagagaaga ggtgcacgaa tgaataattt tgatatattc cgcttcaaac
    14581 cgtttatcaa tgaagcgtta accgcaaagg gcttcaaaca gcccacagag gtccaggaac
    14641 gtttgattcc tttaattgca aaaggaaaaa atatcattgg gcaatctcaa actggtacag
    14701 gaaaaacaca tacgtttttg ttgcctttgt ttgaaaaaat caatgcagaa aaagaggaag
    14761 tacaagttgt tattacagct ccaagtaggg aactagctgc acagatctat gaagcagcta
    14821 cacaaatcgc taaattttct acaccggaga tccgtgtctc gaattttgtc ggcggtacag
    14881 acaaacagcg tcaattaaat cgtctgcatc accaacagcc acaggttgta gtaggcacgc
    14941 ctggtagaat attagacatg atgaatgaac aagcgctgaa gattcacacc gcatttgcat
    15001 ttgttgtcga tgaagcggat atgacattgg atatgggttt tttaaatgaa gtagaccaaa
    15061 ttgcaagtcg attaccagaa aagctgcagt ttttagtttt ttctgccact attccagaaa
    15121 aacttcgtcc attcttacga aaatacatgg aaaatccaat cattgaagaa atcaaaccaa
    15181 aagcagttat ttctgaagac attgaaaatt ggctgatttc aacaaaaggt aaagatgaga
    15241 atcaaatgat ttatcaactg ctgacaattg gtcatcctta tttagcaatc gtatttgcca
    15301 atacaaaaca gcatgtggat gaaattgccg attacttgaa aaaccaaggc ttgaaagtag
    15361 caaagattca tggagacatc accccaagag agagaaaacg agtgatgcga caagtacaga
    15421 atctggagta tcaatatgtt gtagcgactg atttagctgc tagagggatc gatatcgaag
    15481 gtgtctctca tgttattaat gcagaaattc cagaagatct agacttcttt atccatcgtg
    15541 tcggacgtac tggacgtaat ggcttaaaag gaacggccat tacgttatat tcgccaaatg
    15601 atgagcaagc cattgatgcg atagaaaaat tgggcgtctc atttgaacca aaagaaatca
    15661 aaaacgggga aatcgtcaaa acatacgatc gtaatagacg tgccaaacga gaaaaatcaa
    15721 aagatgtatt agatcccacg ctgattggtc ttgtgaagaa gaaaaagaaa aagatcaagc
    15781 ctggttataa gaaaaaaatc gattgggcaa ttgcagaaaa aaataaaaaa gaacgcaaaa
    15841 tcgaacgcag acagcaaagc cgtactgctc gtaaaaataa gaaaaattca cataaataaa
    15901 aaacaagagg ttgtgacaaa aggcgtgtca caatctcttg tttttaagct agactttttt
    15961 tcattccgat acaaagtact tcatcttcaa taaaaggttc tgtgcaagta tgataccctt
    16021 gtttttcata aaaatctaca gccgtcattt cagctactac ataactagtc tgacaacctt
    16081 ctgaaaccgc ccgtttttca gcagcttcaa gcaactgacc gcctaaccct tttcctcggt
    16141 attcttttgc tacgcaaaat cgttccggct tgaagcaagt aggtgttttc ttttcataac
    16201 gcaaagtagc tactggtctt ttttgatcaa gaaatagata ataggtttgt tgcgggatat
    16261 cttttccgtc gaattctagt tctgcaggaa tcccttgttc taatacaaaa acttgatatc
    16321 tcaaaaagca gctagctgcg cgtatccatt catccgctcc aactattttt ttcatcatat
    16381 tcctccttgt catgaatagc agagatgtat tgattccttt attcttatcg taccaaaaat
    16441 cgtggagtat gaccaaaagg aaatgaaaat aaaaattgag atattgacag tgatcaatag
    16501 aatgcgtata ctaattaagt acgaaaagga tatgatttgt tttttaagac tcacagaaaa
    16561 ttctttcttg gctgaaaaaa gaatagtcac taatcaaatt gctccctttt tctattttgg
    16621 tagaaatacc gacgcagctc agcgttatcg agtttaagag gtaatgatta cacatcattg
    16681 caatcaaggt ggtaccgcga gttttcgtcc ttggctgtga tggtgttttt ttgtatttaa
    16741 tttatagaaa gcaggtaatg acatgaaaca attaagtagt gcacaagttc gacaaatgtt
    16801 tcttgatttc ttccaatcta aaggacatac gattgaacct agtgcgtctt tgatccccgt
    16861 gaacgatcca accttattgt ggatcaactc tggtgttgca acattgaaaa aatattttga
    16921 tggttcagtt gtgccagaaa atccaagaat cacaaatgcc caaaaatcta ttcgaacaaa
    16981 tgatatcgaa aatgtcggta aaacagcacg gcaccatacg atgtttgaga tgctgggtaa
    17041 tttttcgatt ggtgactatt tcaaaaatga agccatccac tgggcatggg aatttttgac
    17101 tgatccaaaa tggatggcat tcgatccaga aaaactttat gtaacagttt atccaaaaga
    17161 tacagaagca aaacgcatct ggcatgaaga agttggcctg ccactagatc acattattga
    17221 tgtagaagac aacttctggg atatcggtgc aggcccatgt ggtccggata cggaaatttt
    17281 ctatgaccgt ggagaagctt ataacgattt agcagaagac gatcctgaaa actatccagg
    17341 aggagaaaat gaacgttatt tagaaatctg gaacttagta ttttcagaat tcaaccataa
    17401 gcctgataac acatttgaac cacttcctca taaaaacatt gatacgggga tgggattgga
    17461 acgcatggtc tcaatcgttc aagatgcgtc aacgaacttt gagacagact tgtttatgcc
    17521 tattatccac gccgtagaaa agctaagcaa acaagtaaaa tatggtgaaa acccagtaac
    17581 agatatttct tacaaagtaa tcgcagatca tattcgcgcc ctttcatttg ctattggcga
    17641 cggtgcattg ccatcgaatg aagggagagg gtatgtctta cgccgtttgc ttcgtcgtgc
    17701 tgtcatgcat gggaaaaaat taggtattga acaagcattc ttatatcgtt tagtccctgt
    17761 tgttggggaa atcatggtaa gttattatcc tgaagtactt caacaaaaag aatttattga
    17821 aaaagttatc cgtacagaag aagaacgttt caatgaaaca atcaatgaag gtttggaaat
    17881 cttaaatcaa gtgatccaag aagtaaaaaa cgaaaacggg acgactctag caggaaaaga
    17941 catcttcaaa ctatacgaca catatggctt cccggtagaa ttaacggaag aggtggcaga
    18001 agatgaagga ctgaaagttg atcatgctgg ttttgaaaaa gaaatggatg cacaacgcga
    18061 acgtgctcgc tctgcccgca gtactgaaaa atcaatgggg gtacaatctg ctttattgac
    18121 agatatcaaa gtagaaagta aattcattgg atacacagaa caagaatcag atagtcaatt
    18181 attggtcatc gttaaaggtg agtctctttt gaatgaagtc gcagaaggtg aagctcaaat
    18241 gatctttgat caaacacctt tctatgcaga aatgggtggg caagtagcgg atcaaggatg
    18301 gatcatgaat gaagtaggcg aaatcgtagc agttgttaca gatgttcaaa aagcgcctaa
    18361 tggtcaattt atgcacaccg tagaagtaaa agcaccaatg agtgaaggac aaacctatca
    18421 tctattgatc gatcaatcga tgcgcaatcg gatcatcaaa aaccatacag caacccatct
    18481 actgcacaag gcattgaaag aagtacttgg cgagcatgca aatcaagctg gttctttagt
    18541 cgctcctgga catttacgat tcgactttac ccactttggt caaatcactt cagatgaatt
    18601 agctgaaatg gaaagaatcg tcaacgaaaa aatctgggaa gctatcccag ttgaaacaat
    18661 tgaaacagat atcgatacag ccaaaaacat gggtgcaatg gctttgtttg gtgaaaaata
    18721 cggacatgac gttcgagtag taaatattgg cggctggtca atcgaactgt gcggaggcac
    18781 acatgtagca aatacagaaa acatcggaat cttcaaaatc gtttctgaat caggaattgg
    18841 tgcaggcgtg cgacgaatcg aggctgtaac aagtaaagaa gcgtacgaac tacttgaaga
    18901 agaagaaaaa caactaaaac aagtagcaac aattgttaaa tcacctcaat tgaaagaagt
    18961 tgtttcaaaa gcggaacaat tgcaacagca agtaagagag ctacaaaagg aaaatgaaca
    19021 attagctagc aagttagcga atcaacaagc tggtgatgta ttcaaagatc cagaaacgat
    19081 caatggaaaa acaatcattg cagcacaagt aaaagtgaaa gatatgaatc agctgcgtca
    19141 attagctgac caatggaaac aaaaaaatat ttcagatatt cttgttttag caaacgaaca
    19201 agaaggaaaa gtcagtctgc tagcggcaat gacgaaagaa tcaaatgaag ctggactaaa
    19261 agctggcgac ttgattaaag caatcgcacc gaaagtaggc ggtggcggtg gtggacgtcc
    19321 agacatggca caagctggcg ggaaaaatcc tgctggtatt ccagatgcac ttaaagctgc
    19381 gaaagaatgg gttgcgcaac aatagggcgt ttacgtttga cagaaaaaca aaagttcaaa
    19441 cattgaacga aacatgatta tgggggcgaa gacaaacaag tcttgccctc atactttttt
    19501 caattggatg gcgcatctaa tcaaatggtc ggattcggat ggtctttagt tgacgaaagt
    19561 gacagaaatt tttacgatta gtataaataa aaaggggtga aaaaatgaat agtagaaata
    19621 agacctttcg actagtctta cgtgcgatct tgttagcaat catcatcgta caagcgatgg
    19681 ttccttggct ggggtttatt ccattaggat tcattagttt aacaattatc catatcacag
    19741 taattattgc tgcagtagta ttaggcccaa aagatggcat ggtgattggc ttgttttggg
    19801 gaatcgctac aattgttcgt gcctatgcca tgccaacaac gccatttgat acattagtgt
    19861 tcacgaatcc gattatctcg gtagttccca gagtacttgt tggcttagta gcaggcttgg
    19921 ttttccactg gatttatcaa aaaaataaat caataacgat cagctcagcc tttgcagggg
    19981 tactgggttc attggtcaat acagtcttag tgttaggatt catgggatta ttttatacag
    20041 gtgcaacggc agatgcctat ggtgtagact ctgctctttt atttaaaacc ttagcagggg
    20101 ttgcagcaat caatgggata ccagaagcaa taggtgcggg aatcataaca ccattactag
    20161 caaaagcgtt atttgcagca acaccgttga aaccagaata atgaccatct tttaatagta
    20221 ataggacgca cgatagatag aaaaaggaag agaggagagg aaaacaatcg tcctctccat
    20281 ctttttacct gagcaacaag caaactagaa ggggtaaaaa acttggctaa gaaacaagaa
    20341 gaaaaaaaga aacaaatacc gcaacagcgg acaaaaaaag gagtactagc gggtatctta
    20401 ggattggtta cgaatctgat cttgtttgtc tctaagctaa tgatcgggct tttttcagga
    20461 agtgtctcca ttatggctga tgcagtgaac agtctatcag atactgcttc atccatattg
    20521 actttgatcg gatttaaaat tgctgcaaag ccagcagatc aggaacatcc ttatggacac
    20581 gaacgcttcg aatatatcag tgggttattt gtttcgatca tcattactta tgtaggattt
    20641 caatttttag agtcttctgc tgaaaaaatc atccatccag aaaatgtctc tttaacaccc
    20701 atcgtctttt ttgtcctaat catttccatc tttttgaaat ttgcacaagg caaaatgtat
    20761 caggttattg cgcaatccat tgattctgaa acattgagag ctacagcaac agatagttat
    20821 aatgatgtct ttactacact ggctgtattg ctttcagcag caatcgaatg ggtaactgga
    20881 tggcgtattg atggatatat aggttttgct ttagcattat atatccttta tagtggattg
    20941 acgatgcttc gaaattttgt atacgaacta ttaggaagtc gcccaacgcc agcagagatt
    21001 gaagaaatgg aagagcattt aaatgcttgc gatgatatac ttggctatca tgatttattg
    21061 gtgcataact atggacccaa taaacgcttt gcatcagtcc acatcgaagt agatgaatca
    21121 atgaatttga atgtggctca taagattatt gatctgatag aaaaagactt taaaaagtct
    21181 ttggatgtgg aacttgtctg tcacttggat ccaatgcctg ttgataacga aaaatactat
    21241 aatatcttgc ggcagttaaa gcaaattctt cttgaaacag gtacagggct taatattcat
    21301 gattttagag taattcatca aggaaaaacg ctgcaatttg atgtagtggt ccctgagaaa
    21361 ttccagtttg ttgatacaga actagaaaga atcttacgta gtcaaataga agaaaaaatc
    21421 ggagattacc aattggaaat tacgtttgat cacaattatc ttttatatta aatgagaaac
    21481 tataaaaaga ctccttaaag tttttaattt aaagagtctt tttttctttt aattgtttat
    21541 ggagagcctg acgtgcaaga ttatctgctc ctttattttt gttttcgggc aaccattgca
    21601 ggatcaataa agaaaattcg gatagaagtt cttggattct ttgaaggtag ggaaggaaga
    21661 gctcgttctt cgttgcgttt ttgtcgaccg tagaaactag tgttttgctg tctgaaaaac
    21721 agaaaatcgt ttcattctgt aagtcgtttt tctttagaaa ttctaaagtt tttaaaaaga
    21781 cttcgaactc tgcttgatgg tttgtcccta tagaaagcgg gatagatagc tgttcttgtt
    21841 taccttgata gacaatcaag attccacctc cactaggacc agggttgcct tttgtagagg
    21901 catcaatgta tacttttaac ataaaaagag attctccttt ttgttcaaac taatgttatg
    21961 gtaagattat atcattactg aatcggtgag aggttagaga atgaaaaaaa atattcgatg
    22021 gcagcctgaa ctggcacata cgatcatcta ttggtcattg accttgatca ttctgttttt
    22081 tagtctgatt ctttctttag aaaacacagg tccttattgg aaaagcaatt ttgttttagg
    22141 tatttttctt gtttttctat ttctaggttt gcggcgttct tttattttaa aatcaaactc
    22201 tgtgattata cgttatgctg ctttttggaa agaccgagaa atatctgtcg gacagattcg
    22261 atctttgacg ccaactaata ctggggtgca aatgatgttg caagatgaga aagccccatc
    22321 tgttttcatg atgcgaaaaa aagatcaagt agtccttttt gatcattttg aaaaaaacgg
    22381 acccaaatca gaatcaattc cttatcctta tacgaaaaaa agaaaaagct aattttaaaa
    22441 atcagaacct aaaaaaacga atttttacag gaaaagaaaa gagtgagacg tacggttcat
    22501 cccgtttagc gtctcactct atttgtttat ctattcatgc atactagtat cggtagtctg
    22561 aacgttgcct ttcttgacat gggcagcttg tgttcctcga ttcccttcga tgatctcaaa
    22621 ttcgaccact tgattttctt ccaaagtttt gaatccctct tcttcaatcg cagtaaaatg
    22681 tacaaaaatt tcttctgtgt catcataact gataaagccg tatccttttt tgttgtcaaa
    22741 ccattttaca attcctgtgg tcaaagcact cacgaccttt ctaaagtctg atcatagcct
    22801 tcattataaa gagtatttat aagtacgtca aataaattaa aaacctgtaa ttttatgttt
    22861 tttttctgaa taaaaaaagg tataattggc atatcttaac aaagaagata ttttgttgaa
    22921 taaagttcgt gaaaaggggt aatcaaatga agaatgatct gcaaatttca caagaaacag
    22981 agctagaacc gattgttgaa gtggctagta aattagatct taaggaagac gatttggaac
    23041 tgtatgggaa atataaggcg aaaatcaatt tttctggaat caatcgatca aaggataacg
    23101 ctgaggggca tttgatctta gttacttcca tcaatccaac accagctggc gaaggaaagt
    23161 caacgatcac tgtagggtta ggagacgcgc tcaataaaat caataaaaaa tctgttattg
    23221 cattgcgaga accctcactt ggtcctgtga tgggaatcaa aggtggtgct gcaggcggag
    23281 gatatgcaca ggtattgcct atggaagata tcaacttgca ttttactggt gacatgcatg
    23341 ctatcacaac agctaataat gcattatctg ctcttttaga taatcatatt catcaaggaa
    23401 atgaattgaa catcgatgcc cgccgagtga tttggaaacg ggtagtcgat ctcaatgatc
    23461 gtgaactgcg taaagtaatt gttggcttgg gtgggccaat ccaaggtgtt ccaagagaag
    23521 atgggtttga tatcacagtt gccagcgaaa tcatggctat cttgtgtctt gcggcagacc
    23581 ttgaagattt aaaggctcgt cttgcaagga tcgttgtcgg ctatacattt gaccgtcagc
    23641 ctgtgactgc tggagacttg aaagcagaag gcgctctggc attactgctg aaagatgcaa
    23701 tcaagccgaa tcttgtacaa acaatttacg gaacaccagc ttttgtccac gggggaccgt
    23761 ttgccaatat cgctcatggc tgcaatagta ttttagctac caagacagca ttgagattag
    23821 cagattacgt tgtcacagag gcaggatttg gtgcagatct tggcggagag aaatttttag
    23881 atatcaaagt acctaacttg aaaaaagcgc cggatgctgt ggtgattgtc gccactgtcc
    23941 gtgcattgaa aatgcatggc ggaatgaaaa aagatgaact taaaaatgag aatcttgatg
    24001 ctttgaaagt aggttttgca aatttaaagc gccatatacg taatatggaa caatatcagc
    24061 tacctgtgat cgtcgcaatc aatgaattcg taacagatac agacaatgaa ctcacactgt
    24121 tggagcactt gtgtgaaaat caggggattc ttgccaagag agcgagtgta tgggcagatg
    24181 gagcagaagg cggcgttgat ttagcaaaag ctgtagtacg attgattgat agaaaagaag
    24241 ctaattacaa gccattatac cgcctagaag aaacgataca agaaaagaca gaaacaatcg
    24301 tcaagaaaat ttacggagga aatggtgttg ttttcagcaa aaaagcgcaa aaacaaatag
    24361 aagagtttac gaagaatgga tgggacaaac tgccgatttg tatggcaaaa acacagtatt
    24421 cattttctga tgatccttca ctgctaggtg caccggaaga tttcaccatc acgatccgcg
    24481 aaatcatccc aaaattaggg gcaggatttt tagtagcctt gactggagat gttatgacga
    24541 tgcctggttt gcctaaaaaa cctgctgcat tgaatatgga cgtgacaaat gacggagaag
    24601 tgttgggatt gttttaagct tgattataat ctaccatcga aggagctgta ttttgactac
    24661 agcttctttt tttagacgat agggaattaa ttttgtttac actaggatca ttctttttct
    24721 ttattgtatt ttgagctata ctaaagcata ataggagggt atgatgaaaa cgacatttat
    24781 aagaaaaacc ggtttttacg gtatgggtag ctcgattcag ctaagaatca acaatgaaaa
    24841 acgcagtgtt ctcagtcatg aacaaaagat aacgcttgat ctggaagtgc cctttacgat
    24901 gcaggtcacc ttttattggc taaaaagccc cgtttataca gtgaaagaag caaagcctgc
    24961 ttatgtgatc acaatgaatt acttgattct tcaaatctat ccgtttttat ttctagcaac
    25021 tgggttgggg gccgttttcc tacaaaattt atttttcagt ttttttgcag ttattgtgtt
    25081 gattgcgttc tttttctata taaaaaaccg ggcatatttg atcaaggagg aaaattatgg
    25141 caaactctga tcttttttta atcagtttca atcgaatcga aaaatggatg agagacgaga
    25201 tgggaaatgc acgaaatatg ggatttaccg aacttgtcag acgcctagct caaagaaaac
    25261 aattaatgat cagaaaatac gaagatgatc ttttacagct tgctcagtta aggaatgcga
    25321 tcgtccatga tcggatcgca gtcgattttg tcattgcaga gccaaatgat tggacagtca
    25381 agcgaatcca gctgatcgaa caagaactga ttcagcctga aaccgtgctg cctcgatttg
    25441 caaaacgtgt aacaggtttt gaacaagatc ttccgttact tgaactgtta aagatagtag
    25501 cagaaaaaag gtattcacaa tttccgttat acaataaagg aaaatttgta gcattgatta
    25561 ctttacgtaa tatcgggttt tggctagcaa aagaaagcca aaaaggtccg gttgatttaa
    25621 caaataaacg agcgttggat ttgatcatcc aaaacggaaa gtacacaaat tatcattttg
    25681 ttccagctaa aacacatatt tacgaggttg aagcgatgtt tcgtgagcaa gggactttgg
    25741 aagccgtact tatcactaaa gatgggaatc ccgatggtaa tctgctcggg atcgtacgac
    25801 ctcgtgatat ttataaacaa atagaaaagg attgattttt tgttaattat ttattggata
    25861 ttcagcgctt tgctagtagg agtggatcag ttggtcaaat ggtggatcac tgataatcta
    25921 gcgcttggag agacgaaaaa tcttatacca ggtattttgt cactgaatca tattcgaaac
    25981 actggagccg catggagtat gctagaaggg aagatgtggt tctttactat agtgacactc
    26041 attgcagtcg ttgtgattct cacattgatg attaaaaatc gtgaaaaagg taatcgctgg
    26101 ttcatgattg gattgagttt gattctagct ggtgcgattg gcaattttat cgaccgtatt
    26161 cgtcttggct atgtagtaga tatgttccag acagatttta tgagctttcc gatttttaac
    26221 gtagcagatg tgacacttgt atgtggagtg atttgtatgt taatttatat tattttagat
    26281 gaaaaggatc aaagaaaaaa atgacagaac ttaaaacgac gattcaccat gaatctggta
    26341 ggatcgataa agtattgact aatttattca ccgattacag tcgttcgcaa attcagctgt
    26401 ggctgaaaaa tggagcggtt tctgtcaatg gacagccagt caaagcaaat tacaaagtaa
    26461 aacaaggaga cgagattact gtcttgatcc ccgaaccaga aacattagat gtggtcccag
    26521 aaaatatccc gttgacgatc gtttacgaag atgaagatgt agcggtgata aacaaacctc
    26581 aaggaatggt cgttcatcct tcagcaggtc atacgcatgg cacaatggtc aatgcgctga
    26641 tgtaccatat caaggatctt tctacgatca acggcgttat tcgtccaggt attgttcacc
    26701 gaatcgacaa agatacttcc ggactgctaa tggttgccaa aaatgaccag gcacacgaat
    26761 cattagctca gcaattaaaa gataagacct ctttgcgcaa atatgttgct ttagtccatg
    26821 gagagattcc tcacgaaaaa ggaaccatca atgcgccaat cggccgttcg aaggaaaacc
    26881 ggaaaatgca ggcgataaga gaagatggga agtcggctgt cacacatttt actgtattgg
    26941 aacgtttcga aggatttact ctggttgaat taaaactaga aaccggaaga acacaccaga
    27001 tccgtgtgca tatgaagtat atcggttatc cgttagctgg agatccagta tacggaccga
    27061 agaaaacttt gaaaggcaat ggtcagtttc tccatgcgca aatgttaggt tttacgcacc
    27121 cgactacaaa cgaaaagatg atctttgaag cacctcttcc agaagttttt gaaaaaactt
    27181 tagaaaaatt aagaaaaaac attgcatttt aatttagata acggtatagt aattaacaac
    27241 gaaagcataa aacttaatag aattccttta agtttagtcc cgtgaggcta agaagggtgg
    27301 gagcaaggat acgtatggtg cgccataagt agattgctca ttgacatagt atagctatca
    27361 gtgtatcagt atacgctcag tctaaacatt cctcctgtcc tcttagggca ggagtttttt
    27421 tatgcaaaaa agtaggggga atgaaaatgc aagcaaaaga agtagtagat caggtaacca
    27481 tgaagcgagc attgactcga attacttatg agattatcga gagaaaccat agtatccaag
    27541 atatcgtatt ggtaggtatc aaaacacgag gaatctacat tgcatcaaga attgccgaac
    27601 gcctgaagca gctagaagat atcgatattc ctgtaggaga actggatatc actttatacc
    27661 gagatgacaa aaaagaaaat ccagaagaac cggaacttca ttcttctgac atcccagttt
    27721 ccttagaagg aaaagaagtt attttgattg atgatgtttt gtatacagga cgaacgattc
    27781 gagcagcaat ggacgcagta atggattttg gcagaccaag aaaaatttca ttagcagtcc
    27841 tggtcgatcg tgggcatcga gaattgccaa tccgtgcaga ttatgttggg aagaacattc
    27901 caacatctcg ggcggaagag attttagtag agatgcaaga gcttgatgga caagatcgca
    27961 tcatgatttt gaaagaggaa gattagagga gagagaaaca tgtcggagaa aaaaggattt
    28021 cataatgatg acgtggtgtt agatatccac gatcgcccaa ccccaggaca ttgggtagga
    28081 ttaagtttac aacacttgtt cacgatgttc ggagcaaccg tgttagtccc catcttagta
    28141 ggtatcgatc caggtattgc gctagtcagt tcaggactag gtacattggt gtacttattt
    28201 actactaaag gaaagatacc tgcgtactta ggaagcagct tcgctttcat tgcagcgatg
    28261 cagcttttga tgaaaacaga tggttatcca gcgattgcac aaggagcagt cacaactgga
    28321 ttagtctatt tgattgttgc gctgatcatc aaaaagatcg gttcagcatg gctggataaa
    28381 atcttgcctc caatcgtcgt cggtccagtc atcatggtta tcgggttggg acttgcggca
    28441 aatgcagcaa caaatgctat gtataatcag ggcacgtatg atttcaagta tattgctgta
    28501 gcgctgatca cattaggatt aacaattttc ttcaacatgt ggctaagagg ttttctcgga
    28561 ttgattccga ttttactagg tattattagc ggatacatcg tagccttgct gtttggaatc
    28621 gtggatacta agccaatcgt agacgctgcc tggtttgctg ttcctaactt tgagattccc
    28681 tttgtacaat atgaaccaaa gctatatctt ggagcaatca caacaatggc acccatcgct
    28741 tttgtgacga tgacagagca tatcggccat cttatggtat tgaataaact aactaaacgt
    28801 aatttctttg aagaaccggg tcttcataag acgttgacag gtgatggatt agcacaaatc
    28861 gtggcagcct ttgtcggagg tcctcctgta acaagttacg gtgaaaacat tggtgttctg
    28921 gctatcacac gtgtccatag cgtgtttgtg atcggtggag ctgcagtatt tgcagtcatt
    28981 ttaggattcg taggaaaatt aagcgctttg attttaagta tccctggacc agtcatctca
    29041 ggaatcagtt ttatcttatt tggagtaatt gccgcaagtg gaatgaagat tttaatcgag
    29101 aacaaaatcg attttgataa aaagaaaaat ctgttgattg cttcagttat cttagtagta
    29161 ggaattggcg gactcgtctt tgaagtaggc acatttacat tgtcggcaat ggcattagca
    29221 actgtacttg gcatcatttt aaatcttatc ttaccagaaa cttcacgaag cgaagaaaac
    29281 taaggaggaa acattatgat catcaaatca gaacgtatca gcttaaagca tttattgacc
    29341 gttgaggcac ttactgatca agaagttatg ggcttgatcc gcagagggca ggaattcaag
    29401 caaggtgcta attggactcc gcaaaagcgc cagtactttg ctacaaacct ctttttcgaa
    29461 aatagtactc gcacacataa aagcttcgat gtagcggaga aaaaactagg gctagaagtg
    29521 attgaatttg aagcaagcac aagctctgtc caaaaagggg aaacactata cgatacagta
    29581 ctgacgatgt cagcattagg cgtagacgta gcggttatcc gtcatggtga tgagaactat
    29641 tatgacgaac tgatacaaag taaaacaatc cagtgttcga ttatcaatgg aggagacgga
    29701 agcggacagc atccaaccca atgcttgctt gatttgatga ccatttatga agaatttggt
    29761 cattttgaag gtctgaaagt tgccatcgta ggtgatatca ctcactctcg cgtagctaaa
    29821 tccaatatgc agatgttaaa acgtctaggc gcacaagtat tcttctcagg tcctagagaa
    29881 tggtacgacg atgaattcga agtgtacgga cattatatgc cattggatga actactagat
    29941 caagttgatg tcatgatgat gcttcgggtg caacacgaac gccatgatgg aaaagaaagt
    30001 ttctcaaaag aaggctacca tcaggaacat gggttaacag ttgagcgtgc gaagaagttg
    30061 caaaaacatg caatcattat gcacccagca ccagttaacc gtgatgtaga attagctgat
    30121 tcattagtgg aaggattaca gtcgcggatc gttcagcaaa tgagcaatgg agtattcgtc
    30181 agaatggcaa tcttagaagc agtgctaaat ggaaaagcgt aattatagga ggatgaaaaa
    30241 atgaaaacac tcattaaaaa cggtcagatc aatacacgaa aaaatatgac gacaccagca
    30301 gaaatttgga tagaagatgg gcgaatcaaa gcaatcggta caggattctc agaagccgaa
    30361 tttgatgaag tgtttgatgc aaagggacaa ttgatcacac ctggtttagt ggacgtccat
    30421 gttcatttga gagagccagg atttacatac aaggagacga tcgaagctgg gactcgctca
    30481 gctgctcgtg gcggtttcac tactgtatgc gcgatgccga atttaaatcc tgtacctgat
    30541 acggcagaaa agctgagaca agtatatgac atcattagaa aagatgcagt tgtgaaggta
    30601 ttgcagtatg cgccaatcac agaaaatctg cgaagtgaga aattagttga tcaagaagca
    30661 ctgattgaag aaggtgcttt tgctttcaca aatgacggtg taggtgtcca aacagctggg
    30721 acgatgtatc tggctatgaa agaagctgca aaaaacaata aagccctagt agcccataca
    30781 gaagatgaat ctttattatt tggcggcgtt atgcacgctg gcaagaaggc agaagaactt
    30841 ggtcttcccg ggatcttaag cgtaaccgag tcttctcaaa ttgctagaga tctgttgcta
    30901 gctgaagcaa ctggtgttca ttaccatgtt tgccatgttt ctactaaaga aagtgtccgt
    30961 gtaatcagag acgcaaaaaa agcaggaatc cacgtaacag cagaagtttc accccatcat
    31021 cttattttga tcgacgaaga tattcccgaa gatttcggct tttggaaaat gaacccgccg
    31081 ctaagaggac gggaggacag agaagctttg atcgaaggct tgcttgacgg tacgatcgat
    31141 tgcatcgcta cagaccatgc cccccatgga ttagaagaaa aaagccaaag tttcatgaaa
    31201 tcaccatttg gtatcgtagg aagcgaaaca gcgtttcaac tgatctatac tcattttgta
    31261 gaaactggtc gattcacgtt ggaacaagtg atcaattggt tggcagttaa accggctgag
    31321 atttttggtt tgaatgcagg aacgctaacg gttggtgccc ccgctgatgt agcagttttt
    31381 gatattacac aaacttgcac gatcgataaa gaagactttt tatcaaaagg ggagaataca
    31441 ccttttatcg gctggaaagt aaaaggcgaa acacaaatga cttttgtcaa cggaaaactt
    31501 gtgtggcaga agggagaata aaagtatgga acgtttgctt atattagaag atggaacagt
    31561 tttcgaagga aaggcttttg gtgcagatgc tagtgtcttc ggagaaatcg tttttacaac
    31621 aagtatgact ggttatcaag aaactatcac tgatcaaagt tttaacggtc aaatcattac
    31681 atttacctat cctatggtgg gtaattatgg ggtaaataga gatgattatg aatcgatttc
    31741 tccaacatgc aaaggagtag tagtaaaaga acatgcacgt gtagcttcaa actggcgaaa
    31801 ccaaatgacg ctagattcat ttttgaaaca aaaaggaatt cctggaatat caggtatcga
    31861 tacgcgcgcc cttactcgca aaatccgtga agtcggaacg atgaaagcta gtatagttga
    31921 tgctggagat tccttcgaac atgcatttga tcagctgaaa gcaaccgttc tagcaaccaa
    31981 tcaagtgcag caagtctcaa ctaacaaacc ttatccaagt ccaggaacag gtcggaaagt
    32041 cattgtcatt gacttcggct tgaaacatag tattttacga gaattgagta aaagaaactg
    32101 taacctcaca gtacttccct ataacacaga tgccgagacg attttagcgc tatcaccaga
    32161 cggagtcatg ctgacgaatg gacctggtga tccaaaagat gtgccagaag caatcgaaat
    32221 gatcaaaaaa atccaaggaa aagtaccgat ttttggtatt tgtctcggtc accagttatt
    32281 tgctttagcc aacggtgcag atacctataa aatgaaattc ggacatcgag gattaaatca
    32341 cccggtacga gaagtagcga ccggaagaat tgattttact tctcaaaatc atggctatgc
    32401 agtcgatgaa gccacgattg atccagaaaa actcatcgta actcatgtag aagtaaatga
    32461 cggaacagta gaaggagtca aacacagaga ttatcccgct ttcagtgtgc agtttcatcc
    32521 agatgctgcc cctggtccgc atgatgcact acatttattt gatgaattta tggaaatgat
    32581 ggacgcagga aaggagcagt aaacagatgc cgaaacgtac agatatcaag aaaattatgg
    32641 tgattggatc tggtccgatc atcattggtc aagccgcaga atttgactac gcaggaacac
    32701 aagcctgcct tgccttaaaa gaagaaggat atgaagtggt tttagtaaat tctaatcctg
    32761 cgacgatcat gacggataaa gaagtggcag atcaagtata tatcgaaccg atcacacttg
    32821 aatttgtatc aaggattctt cgaaaagagc gtccagatgc tctccttcca actttaggcg
    32881 gacagacagg cctcaatatg gcaatggagc tggcagcatc aggtatttta gaagaattga
    32941 acatagaact tctaggaaca aagctttctg cgatcgatca agcagaagat cgtgatctat
    33001 tcaaacagtt gatgaaagaa ttgaatcagc caatccccga gtctgaaatc gtcaatacag
    33061 tagaagaagc tgtagatttt gcaaataaaa tcggctatcc tgtcatcgtt cgtccagcct
    33121 ttactttagg cggaactggt ggaggtatgt gtgaaaatga agaagaatta cgtatcgtat
    33181 cagaaaatgg attaagtctt tcccccgcta cccaatgttt gattgaacga agtattgcag
    33241 gttttaaaga aattgaatat gaagtgatga gagattcggc tgataatgca atcgttgtct
    33301 gcaatatgga aaattttgac ccggtgggta tccatacagg tgattctatc gtatttgccc
    33361 ctagtcaaac attgtctgac catgaatatc aaatgcttcg tgatgcttct ctttcgatta
    33421 ttcgagcact taagatcgag ggaggctgta atgtccaact cgctcttgat ccacatagtt
    33481 ttaattatta tgtgattgaa gtcaatccgc gtgtttctcg ttcatctgcg ttagcaagta
    33541 aggcgactgg ctatccgatc gctaagctag ctgctaaaat agcgcttgga ttaacattag
    33601 acgaaatgaa aaatcctgtt acaggaacga cttttgcaga atttgaacct gcattagatt
    33661 atgttgtagc taaaattcct cgctggccgt ttgataagtt tgaaaacgga gaacggcttt
    33721 taggcacgca gatgaaagca actggtgaag tgatggctat cggtcggaat atcgaagagt
    33781 ctttattgaa agctgtgcgt tctttagaaa tcggtacaca ccatatcgaa cttcctgagt
    33841 tggcagacat cacggacgat gacctaatcc aaaaagtggt tcgagcacaa gatgatcgcc
    33901 tgttttatgt agctgaagct attagaagag ggtacaccat agaagaatta gcggatttga
    33961 caaaaatcga tttattcttt ttagataaac tgttgcatat cgtagaaatc gaacagcagt
    34021 tgaaacagca gccgttggat gacaagatat tgaaaacagc gaaacaaaac ggttttacag
    34081 acaaaaaagt ggcggagctg tgggaaataa acgaagaagc cgttcgccag atgagaaaag
    34141 aaaatgattt gattcctgta tataagatgg ttgacacttg tgcagcggaa ttcgaatcaa
    34201 aaacacctta tttttatagt tcctatgaat atgaaaatga aagttcacga tcagaaaagc
    34261 cctcaattct agtactggga tctggtccta tccgtatcgg acaaggggtt gaatttgact
    34321 atgcgactgt gcattctgtc aaagcaatcc agcaggcagg atacgaagca atcatcatga
    34381 acagcaatcc tgaaacagtt tcgactgact tttctatttc ggataaatta tattttgagc
    34441 cattgacctt cgaagatgtg atgaatgtga tcgagctaga acagccgata ggagtaatcg
    34501 ttcaatttgg tggtcagaca gcaatcaact tagccgaacc tttgactgct gctggtgtaa
    34561 aaatcttagg gacaacgatc gaagacttgg atcgtgcgga aaatcgtgat ttattcgagc
    34621 aagcgctgaa agagttggat attcctcaac ctttagggga tactgctaca agtaaagaag
    34681 aagccgtggt tatcgcttcg agaattggtt atccagttct tgttcgtcca agctatgtat
    34741 taggcggacg tgcgatggaa atcgttgaaa accagcgaga tttagaagat tacatggaac
    34801 atgctgtgaa agcatctcca gaacatccag tactagtgga ccgttacttg cttggtggtg
    34861 aatgcgaagt ggatgcgatt tgtgacggtg aaacagtact catcccaggg atcatggagc
    34921 atattgagcg tgctggtgtc cattcagggg attcaatggc tgtgtatcca cctcaaacat
    34981 tatctgaatc gatcaagaaa acgatcgaag attacacgat ccgcttagca ttaggtttga
    35041 attgtgtcgg gatgatgaat atccagtttg tcatccatga aaatgaggtt tatgtgatcg
    35101 aagtcaatcc tcgagcaagt cggactgttc cattcttaag taaggtgaca ggaattccaa
    35161 tggcacaaat tgcgacgaaa gctattttgg gagagaaact aaagacactt ggttatgaaa
    35221 acggacttta tccagaaaca aaagaagttc atgttaaagc acctgtcttt tcttttacga
    35281 aattgcagaa agttgatact tacttagggc ctgaaatgaa atcgactgga gaagtaatgg
    35341 gatctgacag acaattagac aaagctttgt acaaggcatt cgaagcttct ggattacact
    35401 tgcctgaata tggagccgta ttatttacga ttgcggacga aacaaaagaa gaagcactag
    35461 aattagctaa acgttttaca gaaattggct atagtctgat aggaacgaaa catacagctg
    35521 cttatttcga aaaaaatggt ctggtcgttg aaacagtagc gaaaatttct gaggaagcag
    35581 cagaaaaaaa tgtagtcgac ttaataagag atggtaaaac acaagtggtt gtcaatacga
    35641 ttgataaaga tcggggaaat gcttctaaag acggtttcat catccgaaga gaagcagttg
    35701 aacatggtat tccattgttt acttctttag acacagcaga tgcgatcgtt cgagtgatgg
    35761 aatcccgtgc tttttcaact aaccctattt aacggcgagc acatgttaag acaaaaagac
    35821 gaaaaaccgt tgcaggaggg gacagatgaa acaagaaatg atggaaatcg ttcagcaaaa
    35881 acagctggca ccaaagattt ttgaaatgac attgaaaggt gatctagtaa aagaaatgca
    35941 gcaaccagga cagttcttgc atattctcgt gccgcgtgca gacttgctat tacgccgacc
    36001 gattagcttg aatcatatcg accacgaaac aaatacttgc cgtatcattt atcgggtgga
    36061 aggtgagggg actagagtct tctctactct ttctgccgga gaccatttag atgtcatggg
    36121 tcctttaggc aatggttttg atttgtccaa tcttcataca ggagacgaag tatttataat
    36181 cggtggagga atcggtgtcc ctcctctata tgagttatca aaacagttag tcgaaaaagg
    36241 aattaagcca acgcattttt taggttttgc cacacatgaa gtcgtttatt atgaaaaaga
    36301 atttaatcgt ctaggagaaa caagaatcgc tactgacgat ggaacatacg gtatgcacgg
    36361 aaatgtcggt aatttattgt taggaacgaa gacggaacct acagctgttt ttgcatgtgg
    36421 ttcaaacggg ttattgaaga cagtggaaca gctgttttct tcgcatcaaa atgtccagtt
    36481 atcattagaa tcccgcatgg catgtgggat aggggcttgc tatgcttgcg tttgtcatac
    36541 gccggaagat gaaacaggaa caaaaagtgt gaaagtttgt gacgaaggac cgatttttaa
    36601 aataggagag gtaattatat gaatgagaat cctttagcgg tcaaacttcc tggcctagaa
    36661 ttaaaaaatc ctatcatgcc ggcaagcggc tgtttcggtt ttggcaaaga atacggaaaa
    36721 tactatgatt tgaatcagct tggatcgatc atggtcaagg caacgactcc aaatgcccgc
    36781 tttggcaatc ccacgccaag agtggcggaa acaccatctg gcatgttgaa tgcaattggt
    36841 ttgcaaaatc ctggtctaga cgttgttatg cataaacttc ttccagaatt agaggagtac
    36901 aatgagttac ccatcatcgc aaatgttgct ggtgcttgtg aagaagacta tgtagaagtt
    36961 tgtagcaaaa ttggcgaagc accaaatgtg aaagcaatcg agctgaacat ttcttgccca
    37021 aatgtcaaac atggcggaat tgcttttgga acagattctg atgttgcttt tcaattaaca
    37081 caagcagtaa agaaagtttc ttctgttcct gtctatgtca aattgtcccc aaatgtaaca
    37141 gacatcgttc caatcgctca ggcaatcgaa gctggagggg cagatgggtt tacgatgatc
    37201 aatactcttc ttggtatgcg gattgacctt aagacaagaa aaccgatttt agccaatcaa
    37261 acaggtggac tctccggacc agcaatcaag cctgtcgcta ttcgtttgat tcaccaagta
    37321 gcaagtatat ccagccttcc aatcattggg atgggcggcg tgcagacagt ggatgatgtt
    37381 cttgagatgt ttatggctgg agcaagtgct gtagctatag ggaccgcgaa tttcaccgat
    37441 ccgtatatct gtcctaaact aattaaagag ctccctgtgc gaatgagcga actaggcatc
    37501 gaatcgctgg aaagattaat caaagaagtg agagaggaac gtgaacgatg aatcaactag
    37561 accaacgacc aattattgca ttagattttt caactaggca agaggtagaa gactttcttc
    37621 gatttttccc gaaagaagaa aaattgtttg tgaaaatcgg aatggaattg ttctaccaag
    37681 aaggacctga aatcgtacgt tatctaaaag aggctggtca taatgtattt ttagatctga
    37741 aacttcacga tattccgaat acggtcgaaa aagcaatgcg gggattggca aagcttggtg
    37801 ttgatctcac ctgtgttcat gccgctggcg gtattcgtat gatggaggcg gcaatgcgag
    37861 gattagaaga ggggactcca gaaggtggaa aacgtccgct acttctagct atcactcagc
    37921 ttacttctac aagtgaagaa gaaatgcatg ccgatcaact aatcgaagtt tctttagaaa
    37981 aaagtgtgat ccattatgct agctgtgcca aaaaagcagg attagatggt gtggtttctt
    38041 ctgcttggga agtagaagcg atcaaagaaa tggcaggaga cgaatttgtt tgcttaactc
    38101 cgggtataag accagaagga acagtcgcag gagatcagac aagagtagtc acaccttctc
    38161 aggcaaaaaa gatcggctca acttttatcg ttataggtcg accaatcact cagtcaacta
    38221 atccttataa agcatatcaa acgattcaaa cagaatggag ctaacctaaa atgaatgttg
    38281 aacaaagtat cgcaaaagac ttattagaaa ttgaagcagt atttttaaat ccaagtaatc
    38341 cttttacatg ggcaagtgga atcaaaagcc caatttactg tgataaccga atcacgatga
    38401 gctatccaaa agtccgaaaa gagatcgcaa aaggattagc aagcaaaatc aaagaagctt
    38461 accctgaagt acaagtgatt gctggaactg caacagcagg aatccctcat gccgcatggg
    38521 tagcggaaat actggatttg ccaatggtct atattcgaag taaggcgaaa gatcatggga
    38581 aaggtaacca aatcgaagga cgtatatttg aaggtcagaa gatggttgtg atcgaggatt
    38641 tgatttctac aggcggaagt gtcttggaag cagctgaagc agcaaaaaga gaaggtgcgg
    38701 atatccttgg tgttgcggct atctttacgt atgagttgcc aaaaggaaaa gcaaatttcg
    38761 aaaaagccga aatccctctt atgacgttga ccaattattc tgtattgatt gaagcagctt
    38821 tagaagatcg ttatatcgat gagcaagaat taactctgct aaaagaatgg aaaaaagatc
    38881 ctgaaaattg gcagattgaa gcgcgcttga tgaagataga gaaatccgat ttgtgtctcc
    38941 agaatcatca attggaatag caaaaatcca aaaactgatt gcgagaagca tcttttcatc
    39001 aggtatgaaa agaaacttct atcgtgatca gtttttgatt tttttaaaat tcatttttct
    39061 aagactggtt agcagaaaga agagaataat ctcttctctt ttttctatta tatatagatt
    39121 ttctctattt tttggtaagg gagctgtttc aaaaatggta aagagcggaa atgttgcgaa
    39181 tatcagttat ttaatgatat gatattcgga aaaagaaatc tcaaagggag gtaactttta
    39241 tggggttgaa tgaatttatt ttaggattga acaatttaga gacgatcaca agggcacctg
    39301 gattttttaa atatacagag catacagttg cagcacattc gtatcgtgta gcatctattg
    39361 cgcaagttct aggagacatt gaagaagcga atggacaacc gatcgattgg aaactgctct
    39421 atgaaaaatc attgaatcat gactacacag agcgttttat tggtgatatc aaaacgccgg
    39481 tgaaatacgc aagcaaagag ttaagaggga tgctgcaaac agttgaagaa aagatgacgg
    39541 acgaattcat caaccaagag attccagctg agttccaaga agtttatcgc cgtcggttgt
    39601 tcgaaggaaa agatgaaacc gtagaaggag aactattagc catttcagat aaggttgacc
    39661 ttctttatga atcttttgat gaaatcatca aaaataaccc ggaaaaagtt tataaggaga
    39721 tgttcattga ggcagtgact acgattaaaa gctttgaaca tcggccttct gttcagtatt
    39781 tctttaaaga gatatttccg gaactattga accaggaatt ttatggaaaa gaggcgtttt
    39841 tagaaaccgt ctcaacgatt gtaaagaaag aagaaaaata aaaatgatat gagatgcagg
    39901 gggaagtata gttgaaaaag aatatggatg tcgaatgggg ctattctgga gacaaaggtc
    39961 cggaaaattg ggcgagttta tgtgattggt ttgcgagagg tgcggaattt ccattgcagt
    40021 cgcctattca tttgaccaag ggggaagaaa ccaaaataga aggagacacg ctttcctttc
    40081 attatcaaaa gcaacggttt actgacaagg aattcaaaaa cacgattcat ttagtacctt
    40141 ttgaccaact cagttatgta gagttcaaga aagaacgcta ttatttgaca gatattcatt
    40201 tccacttgcc ttctgaacat atcatcaacg gaaaacagga agatattgaa tttcattttg
    40261 ttcatatgaa cagcaaaaaa gagaatctag ttgtgggtgt catgtaccag ctaatggtac
    40321 aggaaggttg gctgtataat caagaaaatg gggaaagttg gaacttagaa aaacatgagc
    40381 attgggtaaa tccggatgtg ttctttccgg agcaaaaaag ccatttccat tatattggct
    40441 cattgacaac tccgcctacc agcgggccaa tccaatggtt tgtaatggat catgtaggaa
    40501 aactgaacca agagtttctt ctccccttcg atggtcagta tgctcgaccg aacaaccgac
    40561 caatccagcc cttgaatgga agagaaattt attatttcga ggagtttgag caatgaatat
    40621 tcagcagatg aaatacgtgg tcgctgtagc aaacaatggt agtttcagag aagcggcaaa
    40681 gaaaatgttt gtctcccagc caagtctttc agctggtatc aaagaattag aaaccgagtt
    40741 agggatcacg ctgtttactc gaacgaatcg aggggcatat ttaacagaag aagggcaaga
    40801 cttcttaaat cgtgccgaga aaatattggt ccagcttgat acattagaaa ctcactatct
    40861 aacgagtgaa aagagagaca atttttcgat cgctgcccag cattatgatt tcttaggtcc
    40921 tttgaccgca cagctactcg aggaattcaa agacgagatc aaacaatttc ggatcatgga
    40981 gacgacaaca gcgagagtga tcgaagaagt caaagagcag cacagtgaga tcggcattat
    41041 ttatatgaat gagcataatc aagctggtat cagaagatat ttacaacaag gagagctaag
    41101 tggccgcagc attggaaaat tcaaaacgca tattttttta aggcaaggac atccattagc
    41161 tgaacacgca gagatcacca aagaagaatt gttatcttat cctcaagttc gttttacaca
    41221 ggatggaaat aatttcccgt atttttatga agaccttatc gagatccctg atcaagaaac
    41281 agtgatttat acgagtgatc gaggtacttt gatgaatatc gttcttgaaa ctgatgctta
    41341 cgcttcagga tctggtattg tgattggagg gattcgcgag cacttgcggt taatcccttt
    41401 ggcagaaggc gaactaaatg aattttatat catttattcg gcgaaacgtc cgctcagtga
    41461 aatcgctcaa agatttatca aagagcttac tcggttactg gaccagcaga gctaagaaaa
    41521 gcagatgaga acgctgttta ttgaacaccg ttgatcaaaa aagaggggtt gtgacagaat
    41581 tttgtcacaa ctcctctttt tgtagcaaac aatttataac taattgctgt tgtgggaaag
    41641 tacgcaaata tgattctaga aacactcatt gattacctgt tttgtttcat agctgtaatt
    41701 ttttcttctt ctggtgtcac gataatcgtc ttttggtttt cataaataac ataaccaggc
    41761 ttggcgccat ttggttttcg gatatgcttt acctgtacaa gatcaaccgg cacttgggca
    41821 gagtatcggt attttgaaaa ataggcagct aactcggcag cttctgtaat tgtttcatct
    41881 gagggcttgt cacttttaat gatgacgtgc gaaccgggta tattttttgc atgaagccaa
    41941 tagtcggttt ttttagctgt cttcattgtc agctggtcat tttgaagatt gtttttacca
    42001 acaagaatga gcgttccgtc agaagaaaag tattgatcag gctgagatgg tttcttacgt
    42061 ttttgttttt ttaagttctt ctttttcagg tatccttctg aagtcaattc ttctttaatt
    42121 gcttcgatat ccattgggcc ggcaatttcc agttgagata agacagattc aagatattgg
    42181 atttcatctt tggcttcttg gatttgttcg ccaatcagct taacagcgtt cttcagcttt
    42241 tggtatcgat ggaaatattt ctgtgcattt tgatttggtg tcaatgccgg atctagtgct
    42301 attttgattg gccgatcttc ttcataataa ttaggtaaaa caacttcttt tgcgccgcgt
    42361 ggtacttgtg tcagaaaagt agtaagtagt tcgccatttc tacggtaatt ctcagcgttt
    42421 tcggattctt tgagtgtttg ttcacgtttt ttcaatttat ttttatttcg ttttagttca
    42481 ttttcgattt ttcgaatcag ttctccgcct tgttgttttg cccgatcttt ctctgctttt
    42541 tcgtgataat agctatctaa taattgactg agtgttggat aagctgttac agcagatgcc
    42601 tgttcggaca atacctgata agcaatcggt gtgaaaaaat ctttattttc cgtttcgtaa
    42661 aaagtaggaa taggctgatg gctgatggct gagaaaaatt cgtcccatac aaccatcttt
    42721 tcattaggac gttccagtaa tcttttggtc agctcttgag cgatatcgaa accgattccc
    42781 tgaaactgac gttggattgc ttttggatct agttcagttt gggaaagccg ctgaaagatt
    42841 ttttctttct cggagctaaa tgggttcaag acctcttgtt ttggcggagc aacatattct
    42901 acaccaggaa gcaaagaacg ataagtgttt tgagagcttc caatgtgttt gatcgcatcc
    42961 agtatttttc cagtttctcg attaactaag acgatcgtac tatgacgtcc cattaattca
    43021 acgatcaata tgatattttg tagatcaccc aactcgtttc gtttagcaaa gtggaaatgg
    43081 atcactcgat cattttcgat ttgttcgatg tcttctaaaa tagcgccatc caaatatttt
    43141 cgaagcatca tcacgaagtt aggcggatta tcaggattct gataatcgat ttgggtaatc
    43201 tgtacacgag cataacttgg gtgcgcagat aataataatc gatggttttt gccgcgtgaa
    43261 cgaatcacca gcacgatttc attttcataa ggctgatgga ttttcgagat ccggccagat
    43321 agtaacgttt cacgcaattc gttgaccatc gcgtgggtaa atacaccatc aaaggacatg
    43381 gttcattcct catttctttt cataagtata ttcattataa cgaaaaaaag aagaaaaacc
    43441 aatttggccg aatagtaaag caaaagtcta gaaaaatctt ggactaatta cataaaaaac
    43501 agtgtttcta tcgttgaaat ccaaattttt aggta
//
LOCUS       DARBEC010000014        41675 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.014, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000014 DARBEC010000000
VERSION     DARBEC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41675
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.014"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..283)
                     /locus_tag="SCL80_000716"
     CDS             complement(<1..283)
                     /locus_tag="SCL80_000716"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEG1106607.1"
                     /translation="MSISFSNNGRKSFSGKEVQIQYAALDIYWNIFSSTATEFYDEDP
                     QVVAFMMNTFFKKEVLDRLNSGMIDKLYFLLKICKENFPATSVKDVQKEF"
     gene            complement(290..724)
                     /locus_tag="SCL80_000717"
     CDS             complement(290..724)
                     /locus_tag="SCL80_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106608.1"
                     /translation="MQTQEAIKTFILKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISFSQQTNYSASYLYTKMKKINAFLA"
     gene            872..2013
                     /locus_tag="SCL80_000718"
                     /pseudo
     CDS             872..2013
                     /locus_tag="SCL80_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(2139..3218)
                     /locus_tag="SCL80_000719"
     CDS             complement(2139..3218)
                     /locus_tag="SCL80_000719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1106609.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(3321..3761)
                     /locus_tag="SCL80_000720"
     CDS             complement(3321..3761)
                     /locus_tag="SCL80_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1106610.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(3818..4228)
                     /locus_tag="SCL80_000721"
     CDS             complement(3818..4228)
                     /locus_tag="SCL80_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1106611.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(4408..4842)
                     /locus_tag="SCL80_000722"
     CDS             complement(4408..4842)
                     /locus_tag="SCL80_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1106612.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(4942..5790)
                     /locus_tag="SCL80_000723"
     CDS             complement(4942..5790)
                     /locus_tag="SCL80_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1106613.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            6179..7945
                     /locus_tag="SCL80_000724"
     CDS             6179..7945
                     /locus_tag="SCL80_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEG1106614.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            8047..9306
                     /locus_tag="SCL80_000725"
     CDS             8047..9306
                     /locus_tag="SCL80_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1106615.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            9453..10754
                     /locus_tag="SCL80_000726"
     CDS             9453..10754
                     /locus_tag="SCL80_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106616.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            10757..11608
                     /locus_tag="SCL80_000727"
     CDS             10757..11608
                     /locus_tag="SCL80_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106617.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            11621..12436
                     /locus_tag="SCL80_000728"
     CDS             11621..12436
                     /locus_tag="SCL80_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEG1106618.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            12587..13753
                     /locus_tag="SCL80_000729"
     CDS             12587..13753
                     /locus_tag="SCL80_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1106619.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            13983..14630
                     /locus_tag="SCL80_000730"
     CDS             13983..14630
                     /locus_tag="SCL80_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1106620.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            14714..15658
                     /locus_tag="SCL80_000731"
     CDS             14714..15658
                     /locus_tag="SCL80_000731"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEG1106621.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            15793..17244
                     /locus_tag="SCL80_000732"
     CDS             15793..17244
                     /locus_tag="SCL80_000732"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEG1106622.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            17265..18101
                     /locus_tag="SCL80_000733"
     CDS             17265..18101
                     /locus_tag="SCL80_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1106623.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            18101..18760
                     /locus_tag="SCL80_000734"
     CDS             18101..18760
                     /locus_tag="SCL80_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1106624.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            18886..19575
                     /locus_tag="SCL80_000735"
     CDS             18886..19575
                     /locus_tag="SCL80_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106625.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            19581..20537
                     /locus_tag="SCL80_000736"
     CDS             19581..20537
                     /locus_tag="SCL80_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEG1106626.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHRIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            20581..21093
                     /locus_tag="SCL80_000737"
     CDS             20581..21093
                     /locus_tag="SCL80_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106627.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(21349..21897)
                     /locus_tag="SCL80_000738"
     CDS             complement(21349..21897)
                     /locus_tag="SCL80_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1106628.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(21986..23166)
                     /locus_tag="SCL80_000739"
                     /pseudo
     CDS             complement(21986..23166)
                     /locus_tag="SCL80_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
     gene            complement(23402..24910)
                     /locus_tag="SCL80_000740"
     CDS             complement(23402..24910)
                     /locus_tag="SCL80_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEG1106629.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            25070..25261
                     /locus_tag="SCL80_000741"
     CDS             25070..25261
                     /locus_tag="SCL80_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106630.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            25464..26381
                     /locus_tag="SCL80_000742"
     CDS             25464..26381
                     /locus_tag="SCL80_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEG1106631.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            26374..27447
                     /locus_tag="SCL80_000743"
     CDS             26374..27447
                     /locus_tag="SCL80_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106632.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            27595..28113
                     /locus_tag="SCL80_000744"
     CDS             27595..28113
                     /locus_tag="SCL80_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106633.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            28094..28486
                     /locus_tag="SCL80_000745"
     CDS             28094..28486
                     /locus_tag="SCL80_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEG1106634.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVFFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            28562..29497
                     /locus_tag="SCL80_000746"
     CDS             28562..29497
                     /locus_tag="SCL80_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEG1106635.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            29630..30409
                     /locus_tag="SCL80_000747"
     CDS             29630..30409
                     /locus_tag="SCL80_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1106636.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            30402..30719
                     /locus_tag="SCL80_000748"
     CDS             30402..30719
                     /locus_tag="SCL80_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1106637.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            30963..32573
                     /locus_tag="SCL80_000749"
     CDS             30963..32573
                     /locus_tag="SCL80_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1106638.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            32730..33578
                     /locus_tag="SCL80_000750"
     CDS             32730..33578
                     /locus_tag="SCL80_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1106639.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
     gene            33809..34288
                     /locus_tag="SCL80_000751"
     CDS             33809..34288
                     /locus_tag="SCL80_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106640.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNTDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
     gene            34321..36549
                     /locus_tag="SCL80_000752"
     CDS             34321..36549
                     /locus_tag="SCL80_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1106641.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKGRTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            36678..38321
                     /locus_tag="SCL80_000753"
     CDS             36678..38321
                     /locus_tag="SCL80_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEG1106642.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKLALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(38394..38939)
                     /locus_tag="SCL80_000754"
     CDS             complement(38394..38939)
                     /locus_tag="SCL80_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEG1106643.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(38962..39447)
                     /locus_tag="SCL80_000755"
     CDS             complement(38962..39447)
                     /locus_tag="SCL80_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1106644.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            complement(39437..40249)
                     /locus_tag="SCL80_000756"
     CDS             complement(39437..40249)
                     /locus_tag="SCL80_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEG1106645.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            complement(40388..41500)
                     /locus_tag="SCL80_000757"
                     /pseudo
     CDS             complement(40388..41500)
                     /locus_tag="SCL80_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
     gene            complement(41521..>41675)
                     /locus_tag="SCL80_000758"
     CDS             complement(41521..>41675)
                     /locus_tag="SCL80_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEG1106646.1"
                     /translation="SLEKLGCQNFNSRSVKGLESRFESILLAYSVLLSRAQRRFEGTL
                     RYSLGY"
BASE COUNT        13845 a         7534 c         8109 g        12187 t
ORIGIN      
        1 tgaattcttt ttgtacgtct ttcacagaag ttgcaggaaa attctcttta catattttta
       61 atagaaaata taatttatct atcataccgc tatttagtcg gtcaagtact tcttttttaa
      121 aaaaagtatt catcataaaa gccactactt gcggatcttc atcataaaat tctgtagcag
      181 tacttgaaaa gatgttccaa taaatatcta atgctgcata ttgaatttgt acctcttttc
      241 cagaaaaaga ctttctgcca ttattagaaa aggagataga caaaccgtac tatgctaaaa
      301 atgcattaat ctttttcatt ttcgtataca aataagaggc actatagttt gtttgctgac
      361 tgaaactgat aatcgaattt gtctgacagc tgaacaaaga agtcaagatc tgataactga
      421 ctgattcgtt gcagtatctt ccaaatagac gataatagca gtaagccgga tcaattcctt
      481 caaaattcaa ctttaaaaaa ggtttttgct tatcaatatg cagttctgtt agattgagac
      541 gctcaaagtc ttgttctaat tctttgatgt atagtaaaaa atttgattca gacatatgga
      601 aatgatctgc taaatattgt gtagtaataa agaaacgatg ttcagatagg aaaagaaaaa
      661 tatctaattt tgtattgctt tttttcttca agataaacgt tttaatcgct tcttgagttt
      721 gcatgaaaga ggctcctttt tacttaaatt gttaaaactt gaaaatatta ctgttttttt
      781 cataaattta gacaaaaaaa cagtaaacat ataggtagaa tctctactat cttatttctt
      841 gcatacaaaa aaatcaagga gtgagagagt tatgagatat tcaaccatct taatatcaac
      901 atttgcagcc ttaagcttat cgcttactat aaatcagaca gtccacacag aggagacaga
      961 aacagccaaa tggacagaaa ccaagtgggc caacgtcaat cgaattacct ttcaggatga
     1021 attttcatcc ttcaattcag gagtcgcaaa attaagaaaa ggacaaacag cgaccatcaa
     1081 agcaaaaatt gactattcgg gagacttagc acctatacta caatcagctg ttacatttga
     1141 gaatgttgat cccactttgt cgattggcta tgacgagaac actttgacta tccaacacaa
     1201 gcaagcgata tttaccttca ccgttacatt aaatcaggac ttgttaaaac cagcgctttt
     1261 tactgtcaag gtatcagatg gactaccgaa cgatcatcat ttaacgccgt atagtcagcg
     1321 gcagacaatt gagcaagatt cagccatcga ctcaggagga gatcttgttg aagaaccaac
     1381 agataaacca gaaaatgaaa ataagccaga agtaccgcct acagaaaatc cagacggaga
     1441 acagaaaccg gaaatagaac ctggagaaga acccgataca gaaactcaac cagagccaga
     1501 caataaatca aaacctgaaa taacccctgg agaaaaacca gatgtaactc cagaaccaga
     1561 tacggattct ggaaaccaaa cagtaccgga aacaaatcca gacacagata atgaaactga
     1621 aaatccagaa aaaccagagg tagatccaga agaaaaacca gatgtgactc cagaaccaga
     1681 tacggacgca agagaccaag gaataccaga aaaaataaac aaaaaaacaa ttcaagagga
     1741 tggaaaaaaa gagagtaaaa aatcaaatct agcaattcta aaaataaatg aagagcagct
     1801 gaataaaaaa tcacgaatat ttgatagcgc ccaatcagct gaaaccttaa aaagtagcaa
     1861 ggatacaacc tttgctgtcc tgaaacgaaa aataaacagc taccaaaatc cggagagtca
     1921 caaaacaaag ttatactgtg gagcggaatc attctattaa gtatagcaac aatgctttct
     1981 gcaaaaagat ttaaacaaaa cagatctctc taacatttga ataatgtctg tgaacgcatt
     2041 tttcaaataa aaagaaatat ccagaaaatg accccaaaat accagattga aatctgatat
     2101 ttggggtcgt tcatacttta tctttcttca tatttatttt aagggatatc tgtttctttt
     2161 ggaagatggt tcgggatctt tttcactttc aatacatcta aaaagaaggt aaagatcgga
     2221 atgccgacaa tcaagcccca aacgccaaag aaccgttcgc taacaagtaa gaccacaaat
     2281 gtataaaaaa tcggcagttc tgttttgctg gacatcaact tcggattcag cacataagac
     2341 tcaattagat ggacaatcaa aatcgtaatc aggatataaa caacatctcg gatgcctcct
     2401 tgggaataag ctatgaaaga tagaggaata caagaaatga taacacctgc tactgggatc
     2461 agacttaaaa tgaaaatcat gatcgctaaa ctcagcagct gatgaaagcc aaatacagcc
     2521 aaggcgattg tagtgagcaa cgtatttaaa agagcgacaa caaattgagc ttctaatacc
     2581 aaaccaaatg tattgacaaa cttatcggca aaatagtaaa tatcttgaaa gaaccaagaa
     2641 aattcacttt ttaaaaacaa tcttgagaat acaattgttt ttttcttttc aatcataaaa
     2701 aagaaactta agatgaatga catagcaaac gccactgcca tttttcctat atcctgtaaa
     2761 tagccaagcg caatcgaagc gccattttga agctgttcaa aaaaatcgtt tcgctcaata
     2821 taagtatgga tatattgtaa aacaggatct gcatcttttg gctgatgctc gtaaaaatta
     2881 ataacggact ggatcatgtc aaatgtttga tgaataagca tcgggatata ctttgtgaat
     2941 gccagataga taaggtaaac gatcagtgta taaatcatga tagctaaaac acctgtaggg
     3001 attcggacaa atcgctgaat gatttgaaca agatgaatca tcaaataagt aaagataaat
     3061 gtgagcaaaa tggttgtaat catacttcta actaagaaaa gaacaaaaac gacaaataat
     3121 aaaacagtga gacggcgtat tttttcattc gccaaaaaac gctgataaac ggtcaaatcg
     3181 acaacctcca aataacaaaa aataaacacg ttattcatta tatgatggaa tatgaaaata
     3241 agaaagagca aaaagaaaaa ccgctaaatg acgttatctt atgagttggt tttcctcata
     3301 aaatcgttca tctagcagtt tcagcccttt ttctggtctt gatgaaaatt atctagcatt
     3361 tttcgggtca gatgttgcag tgtatccaat tcttcctgtt caagtggaag ataagacttg
     3421 atcttttgtg gaataccttc tgcttgtttt tgcaacgcag caccttgagc agtcagcgta
     3481 acatttacga ttctttcatc acccgtttca cgctgacgat taatataatt tttttctgct
     3541 aactttttta gtaagggagt cagcgtacca gaatccagac acaaatcatt tcctagctct
     3601 ttcactgaag tctttttctt ttcccataaa actaacatca caagatactg ggtataggta
     3661 agattcagcg gcttgaggag gggagtgtag tgtctaatga tttctttaga ggcggcatat
     3721 aaaggaaaac atacttgatt aactaaatat agatcttcca tttttccacc tccgctgttt
     3781 tactatagtg tatcatagcg gatttacaca aaaaagtcta ttcactaact gctttcagag
     3841 taacttctat atttccagct gtagcttttg aatacggaca cactttatgc gctgcctcta
     3901 ataattcttg cgtttcttcc agagaaagac cttcgataga tccttcaatc tctacaccta
     3961 agacaaagct cgtttcactt tctgaataaa gggaaacttg tgcactgaca gtagaagccc
     4021 cagagatatt tttttgctgt ttgatcaatt ctaatgcact gttaaaacat gcactgaaac
     4081 cagctgcaaa caattgttca gggttggttg cattttcgac gcgtttacca ggagctgcaa
     4141 tattcaattc gaatgtacga tcgggattat gcacttctcc agagcgtccg cctgtgttga
     4201 tcattttcgt agaatacatt tttttcataa aattaccacc tttaattata ttttatacaa
     4261 ttgaatttta cacaatataa ttgtggaaag caaagaaatg gattggcata agataaataa
     4321 gagaaagcac atgaaacaga cgcgctttct cgaatagcaa acatatacaa aaaatatcag
     4381 agacaaaaaa taatctcatc ataaacatta catatatttc tcaaaaagtt gttttctttc
     4441 ttcaaaaatt acttcacgat ggacctgatg aagcatccat aaataaccaa aaggatctct
     4501 gaacattgag ttagtcacac caaagtccat catttctgtc atttcttgaa tctctacaca
     4561 gccatttcgt aatgctttat tatgtgtgtc ctcgatatca gagacgatga tattgaacca
     4621 gatcgtattt ggatgttcag cagtaggagc agtcaaacca agagacgtgt tttcatccag
     4681 catatggaat ctcgtaccat acaaggtaaa aatgacttca ttattgcctc tagggtaatc
     4741 tgttgcctca atcagttgta tttcatcaaa gattgtttga tacagttcta gtgctttcaa
     4801 actatcattt aatacaaaat cgatttccgt tcctttatgc ataaagatct cctctccttt
     4861 tcagtcgaac agttactaga ttcttttatt ataccagcta aagaaaacgc attcaaaaaa
     4921 atagcggatt tacttaccaa ctcattcgat tggatgataa gtaaatccgc gtcccattac
     4981 ttctgctacg ctgctgacaa tgacaaaggc acttggatcg atttcttcta tcaatggttt
     5041 cattcgggaa aattcatgat cagtcacaac gatcaataaa acctcttttg actttcgatt
     5101 atacccgcct tttgcctcta gcagggtaag gccacgtcca atctcagcac ttaagcgaag
     5161 gcggatgtct tccaaataat gttcactcat gaccataaca gatttctttc gattgatacc
     5221 tgtttcgata taagtcatga caattgaagt gattgcaata gataaaatag cataaagaaa
     5281 ctcttctatt ccaaaaataa acagattaag agaaacaacg aatgcgtctg taaaaaataa
     5341 gccaatcgaa gtattcaagt ggaaatattt tttaaaaatg aggggaggga tcgtcgtacc
     5401 gccgctagaa gagttgtttt tatataaaat cgcaactcct aaagcaaaaa tcgcacttcc
     5461 aaaaatgaca gacaacaacc gatctgaagt aatcattgtt tctggaacta ttgcaatgat
     5521 taaaggaaaa accaagcttc cgaaaagtac tttaaaaaag ggttttttac ctaaaaatag
     5581 aagagctagt accaacataa cgatattcaa cactaaaata actatagctc ggtcaaagcc
     5641 taaagcagct tccagtaata ttcctagacc gctgacacct ccagcagcaa tatgatgagg
     5701 ccctaaaaac atattgatag cgactcctaa tatgaagagg gcaaaaataa tcttaatgta
     5761 tgtccatata aattgtctcg tttctttcat ttattcactt ccttaattca ttaataatac
     5821 tcttatctta ttataactta cttttcacct taagttaact ttaattaata aaaaaattag
     5881 gctagtctaa attattatgt ttcattcttc tagaatatac ttctacgcca aaaactgtta
     5941 aaaaactttt actttagaaa attggagaaa ttgatcgctt aagataaatt caggacacta
     6001 aatagtacag gaagtagact aaaactttta aaagttttaa gtctatctat ttggcaagtt
     6061 taaaaaataa aaattcggta tcaaaaaact gttaccgata acatttttga aagcgttgtc
     6121 ttttgcttct ctatcactta tagtgatgac gtcatattaa aacacaagga ggaaatcaat
     6181 ggatacagct gcaatttatc atcgaccgga aagtgaatat gcatttctat atacgaatac
     6241 aagatttcgt attcggcttc gtactaggaa aaatgatatc aaaaaagtat atgtgctatg
     6301 tggagatcca tataccataa caactgaaaa atggtatcaa aaacaacgac caatgaaaaa
     6361 atgtctaagt accaatgttc atgattattg ggaaattgaa gtgacgagcg aaacaagtcg
     6421 cttacagtat gcgttccatg ttgttgggga agataacacg gactgttttt acggtgatca
     6481 aggaattttt ccttatcgag aaaatgtgat tactgaacca aatttttatt ttcgaatacc
     6541 ttattttcat caaatcgatc gattcactat tccagaatgg gtaaaagaaa cagtatggta
     6601 tcaaattttt cccgaaagat ttgcaaacgg ggacaaagca aatgaccccg aacacacttt
     6661 accttgggga agtaaagatc cagaccgaga agactttttc gggggagatt tgcaaggtgt
     6721 gatggatcat ttggattact tgacggacct tggcatcaat ggcatctatt tttgtcccat
     6781 atttaaagcc acatccaatc ataaatacga tacgattgat tattatgagg tggatcccgc
     6841 ttttggcgat aaaaaacttt taaaaaactt gattgatgaa gcacataaaa gagggatacg
     6901 tatcatgttc gatgctgtat tcaatcatat gggtgtccat tcaccacagt ggcaagacgt
     6961 tctcaaaaac ggagaaaaat cgatctataa agactggttc cacatccgtt ttttcccagt
     7021 agattcttat cagatgactg acttaccaga aactgctgaa aatatcccat atgatacttt
     7081 tgcttttact ccattcatgc cgaaactaaa tacagcaaat ccagaagtgc aaaaatattt
     7141 gctggatatc gcaacttatt ggataaaaga attcgatatt gatggatggc gtcttgatgt
     7201 tgctaatgaa gtcgatcatc atttttggaa aaaattcaga gaagcagtta cagaaatcaa
     7261 accagacatc tatatccttg gagagatttg gcattcctct caagcttggc tgcaagggga
     7321 tgaattccat gcagtgatga attatgcatt tacagattcg attaaagatt attttgcaaa
     7381 aaagaaaatc acagcttcac aaatggtcag tggaatgaat catcagcaaa tgctttacag
     7441 agatcaggtg aacgaaggaa cattcaactt attagattct catgatactg ccaggatctt
     7501 gaccctttgc caaggtaaca aagaattgat gaagtctgtg atggccttta tgtttcttca
     7561 aaaagggtct ccttgtatct attacgggac agaaataggg atgactggtg aagatgaccc
     7621 ggattgccgc aaatgtatga tatgggagaa ggaggaacaa gatctagagc tattcggttt
     7681 tataaaagaa cttgtttctc taaggaaaca gttgtctaag attatttctg aaggttctac
     7741 tcagtggcta atagtaaatg atcgtgaaga taagctctat ttcacacgga aactagaggg
     7801 acagatcatc tatgtttact ttaatcagtc aaaagaacct tgggtagtag aacaggaaaa
     7861 tgaggtgatt cttagtcaaa attgtcagtt gttagaagac gggaaagcag agatacgaca
     7921 atacggattt ttgatcatgt gttgaaaact gttaccggta acaacaagaa agcgtttgca
     7981 aatgaagatt tgtgcccatt ataataaggg tgtaacagca cgttgaatac aaaggaggaa
     8041 atgaagatga aaaaaagaag tgtgaaacag ttgggtttag ggttaatctc tttaggaatc
     8101 ttaggcggat tagcggcttg tggcaatggt aactcttcag cagaagggga agccgatgaa
     8161 ggaaaaacgt taacagtttc cgttgattct ggatataaag attatataaa cgaaataaaa
     8221 aacactttcg aaaaagaaaa cgatgtgaaa atcaagctag tagaaaagga catgttcgat
     8281 cagttggaat cattagcgtt agatgggcct gctggaaagg gacctgacgt tatgatggca
     8341 gcatatgacc ggatcggtgc gttaggacag caaggacatc tggcggaagt aaaactggga
     8401 aatgaagcag cttacgatga aacagacaaa gcccaagtga cttatgatgg aaaaatctac
     8461 ggagaacctg cagtaatcga gacccttgtt ttgtactata acaaagattt agtcgataca
     8521 gcaccatcaa cgtttaaaga tttggagaat ctatccaaag acagtcgttt tgcttttgag
     8581 tcagaagcag gaaaaaatac aggattttta gctaaatgga ctgactttta ttattcctat
     8641 ggtttaattg caggttacgg tggctatgta tttggtgatg atggcacaaa tccttcagat
     8701 atcggtttga ataatgcagg tgctgtagaa ggaatctctt atgcaacaga ttggttcaaa
     8761 aatgtttggc caaagggaat gcaagacata aaaagtgcag gagacttcgc cagccagcaa
     8821 ttcatgtcca ataaaactgc agcaatcatt gacggtccat ggcaagcgca aacatacaaa
     8881 gaaaacaata tcaattatgg tgtagccaaa attcctactc taaataatgg acagccttat
     8941 caaccgtttg gcggaggaaa aggatgggta gtcagcaatt atgctaaaaa caaagacctc
     9001 tctcaaaaat ggcttgatta tgtaacgaat caggaaaatc aagaaaaatt ctttgaaatg
     9061 gtaaatgaaa tccctgcaaa ccaacaagct agggaaacag caaaggctaa aaacgatgaa
     9121 ttaacaacag ctgtgattga acaatatgaa acagctcaag cgatgccaaa catcccagaa
     9181 atgggcgaag tttggacagg tgcagaaaac ttgatgttcg atgcagcatc aggttcaaaa
     9241 acaccaaaag aatccgcaga tgaagcagta aaaacaatca gtgaagccat agaacaaaaa
     9301 tactgattaa aatgataacc gtccattttt ataaaaaatg gaacaggtgc ttcagataaa
     9361 agtggcaaga gcgagatgga aaattatctt gctcttgttg ccattatctt aaaagcagtt
     9421 tgcggtttag caagtttaga gggggcattc agatgcagca agataaaaca agcaatatca
     9481 acgaaaaaaa aatcacgctt tgttcactta ttccagggat ggggcagttt gtaaatggcc
     9541 aagcttttaa aggtatcgtt tttttcgcca tattcgtcct attcattggt cagatgcttc
     9601 tgggcggatt tcatgcattg gtaggattgg ttactttggg aagtatacca atggaagatc
     9661 attcattatt tattttgatt gaaggcactt tgcaattcat cattacaatc attttcttga
     9721 ttttttacgg tttgaatctt ttagacgctt atcgagtagc gaagatgcgt caagagggca
     9781 aaaaaatcaa tcggacgatg aaggaagtca tacataatat aggtgatgaa ggttttcctt
     9841 atctattgac acttcccgct tatcttctca tgatttttac gatcattttc ccagtcctag
     9901 ttacattgtt cactgcattt actaattatg acttcaatca tattccacca gccagtttaa
     9961 ttgattgggt aggggctaaa aactttttta gtatcttctt tttaagctca tatcgtaata
    10021 ctttttttgc agtcttttcg tggaccgtga tttggacttt atgtgcaaca actttgcaaa
    10081 tcacccttgg tattctcaca gcggttgttg ctcaccagaa atttattaaa ttcaaacgta
    10141 ttttcggtat cattttctta ttaccatggg cggtcccagc ttttattact atcatgagtt
    10201 tttcgaatat attcaacgat agcgtaggtg ccatcaatac tcaagtgatt cctttcataa
    10261 accagcttcc ttttgtagat atcagtgcaa tctcttggaa aacggacccg aactggacga
    10321 aagtagcaat tatcttagtg cagggctggt taggatttcc ctatatctat gtgatggtta
    10381 caggaatctt acaatcgatt tcagaagact tatatgaagc agcaaaaatc gacggagcaa
    10441 atgcatggca gcgcttttca aacatcacgc ttcctgctgt cttcctagtg gcagcgccaa
    10501 catttattac acagtatacg gggaatttca ataatttctc catgatctat ctgtttaaca
    10561 atggcggtcc cggaagcgta ggcggaggag ctggttctac tgatattctt atttcctgga
    10621 tctataaact tacgactgga acttctccgc agtattctat ggcttcagca atcacgttga
    10681 ttatctccgt catcgttatc tgtgtgtcat tgctggtatt taaaaagaca aatgcgttca
    10741 atatggggga ttaacaatga aaaaatttca ttcgagtgct cgattccaaa aacgactttc
    10801 tcactttttt acttactttt ttcttttaat actgtcagtc attatcatct atccgctttt
    10861 gatcacagca agtactgcct tcaagtcagg aaatattgca gcttttagtt tagatctttc
    10921 aagtgactgg actttaggaa actttactcg gctattcagt gaaacacttt acgggacttg
    10981 gtataaaaat acattgatca tttctgtcct aacaatgatc gtacaagtga ctgtagtaac
    11041 tttggcgggc tatacttata gtcgctatcg ttttatcggc cgcaaatcaa gtttgatgtt
    11101 ctttttagtg attcagatgg taccgactat ggcagcattg actgcctttt atgtcatggc
    11161 tttcttatta gaagcgctcg ataagtactg gttcttgaca atgatctata taggcggtgg
    11221 aattccaatg aatacttggc tgatgaaagg atactttgac actgtaccaa tagatttaga
    11281 tgaatctgct aaacttgatg gggcagggca tttcaggatt tttttccaga ttgtattacc
    11341 gcttgtcaaa ccgatgctcg ccgttcaagc attatgggct tttatgggac cttttgggga
    11401 tttcatgtta gctaagttcc tgcttcgaac acctgaaaat cagactgttg ctgtcggctt
    11461 acaaacattt atttctgatg tgacaaacca aagagtctct ctttttgctg ctggggcaat
    11521 tttgattgca ctaccgattt gtctattatt cttcttttta caaaagaact ttgttaccgg
    11581 cctgacatct ggcggaacaa aaggatagga gcagagtatc atgcaaacat ttccgttaaa
    11641 ctatttttcc gcattacgta cgccaactca agtgtttgcg ggacgcaaac tattatcatg
    11701 gcctaaattt ttcttgatat ttgtattttt agtttccttg atggtcatgc ctgtgactct
    11761 tttttatgcc aatcagatcc aagcgattcc attagaacaa tttctatccg ttcattcgct
    11821 gatcgatgaa caaggaaccc aaaaattttc tgaacttgaa ttatctgaaa ctgggttaca
    11881 agcatcgcaa caaacgatag ctgtgacccc tgaaattttg gtaggtgtgt ctttatctga
    11941 gaaacaacaa tccgatcatg gaacttttat tgattttgag aaggaacaat gggtgatcca
    12001 acaaaaggat aaatctggta tacgccgcta tacgatgaat tatagcccat ctttccaacc
    12061 agattctgta cgtacgcccg aagatttcca acgattttta gaaagagaat tttatgccag
    12121 taatcgtcca acaatcattt tatcttatag tttaagtctt ggtcttgtcc tatttgtcat
    12181 gacgagtctc attttattcg gggcttcctt tttcttgtgg atgacaagaa aaagccaact
    12241 ttcatccatt catacattca aagagagtgc aaatttgatg ctgaatgtta tgggaatagg
    12301 ttccatggca gcagcagttg tagggtttat ccactttgac tttatcttga tgctaagtgt
    12361 ccaaacaacg ataactgtct tgctgctttt atgggttttt gtcaaaacaa gattcaaaga
    12421 taagcgtgtc gaatgacaag tttaagcgat ttatgcgaca atgaatgtga aaaaaatgaa
    12481 aaataaagta gtttttgaaa aatttctgaa aaaattaaac ttcttagttg attcttttct
    12541 tttttagtat taacgtaata gggtagtttt tttaaggagg aaaatcatgt tagagaaaac
    12601 tttatacaat caattatttt cacgttcttt ctcgctccct gtcgaagtaa attattggga
    12661 cggtacaacg aaacaatacg gagatacgga cactcctccc caaatcaaaa tcaatatcca
    12721 tgaagaaatc ccaatgaaag agattacaag taacgcatct ttggcactag gagaagccta
    12781 tatggatcat cgaatcgaga tcgagggaag tatccaagca ttgatcaacg atgcttatca
    12841 gcaagcaaac agctttttac gaggaaaaga ttatctaaaa tggctgccaa agaaagaaaa
    12901 acatacgaaa caaaaaaaca aagaagatat ccatgcgcat tatgacttag gtaacgactt
    12961 ttataaactt tggctagatc caactttgac ttattcttgt gcttatttca atacacctga
    13021 agatacgttg gaacaagcac aaatcaataa agtccatcat atcttagaca aactctttat
    13081 caaagaaggc gaaacgttgc ttgatatcgg ttgtggctgg ggaacattga tgttcactgc
    13141 taccaaagag tataatgtca aggcaactgg gatcacacta agtgaagaac aatacgattt
    13201 gattaccaaa agaataaaag aagaacattt agaagataaa tgccgtgtat tattaatgga
    13261 ttaccgcgaa ctaaaaggcg aaacgtttga tcatatcaca agtgtaggta tgttcgaaca
    13321 tgtaggttct gaaaatcttg aaggttattt caaagtggta aaagatttat tgaaaccaaa
    13381 aggaacagct ttgatccatg gaatcagccg acaacaaggc ggtgcgacaa atgcttggat
    13441 caataaatat attttccctg gcggttatgt ccctggtgtt actgaattag ttgataatat
    13501 tacaaaaaac gatttgcaac tgattgattt ggaaagctta cgtcgtgact accaattgac
    13561 attagaacat tggacaaaga attttcatca agtccaagat caagtcacga aagaaatggg
    13621 tgattccttc tatcgaatgt gggatctata tttacaagct tgtgcagctt catttgaatc
    13681 aagtaatatc gatgtgattc aatatttgct tgttcaacct ggaaacaatg acatcccaat
    13741 gcatcgtgca taattgaggc aaaagtactg tttaaagact atttaaaaag aagctaagtt
    13801 ttatgatgct ccagaagtga acttttttgt ctaacttcta ggattgtctc taaagcttag
    13861 tttctttttc tttatatttt cataataaaa gtttggttct ttaattttta aacatgacag
    13921 aaaatttttt cttcgttata cttgtaatag aatactattt tataacaaag gggatcgaat
    13981 caatgaaaaa aactgttatt tatactagtt tatttgcagc tttgttttta gctgcttgtc
    14041 aatcaaatac caatcaaggc acatctggga atacgtcttc cagtagcgaa cagcgctctt
    14101 cacaggtatc ggactcctca acaaaagcaa gcacatcttc gtctagccag acaaaaagtt
    14161 ctagtgaagc aagtactcag tcagatatca aagtgtctgc agctcgtgcg atcgaattgt
    14221 tccaagataa atacccagat actgcaatta ctagtttgga attagatagt gattggggat
    14281 cttacttcta caagatagaa ggtgtcgatg atcaaaaaga atacgaagca agaattgatg
    14341 ctcaaaatga aaaaatggag acagagaatc cagaaactct agatcgagat gaacaaaatg
    14401 gtcaaaagaa aaaagaagat ggtctggatg ttgataaatt gatcacgatt gaggaagcag
    14461 caaaaatcgc cgtcgacaaa gtaggaagcg gtacggcaac agattgggat ttggataaag
    14521 atttaggtac tacttattgg gaagtcaaag taaaagacgg caatcaaagt acatcagtca
    14581 agatcaatag ccaaacaggc gaagtactgt caactgaaca agatgattaa gaatttcttc
    14641 ggaaaacaag agttgatttt tcttttagtc atgatacact agacaaaata acttgtttga
    14701 aggagcgatt tacttgagtt tagaaacgac ctttgccaat catatctttg ccaatccttt
    14761 gatgaatgca tctggtgtcc actgcatgac tacccaggaa ttagatgaac tggctcattc
    14821 agaagcagga gcatttatca ctaagagctg tacgatcaat gaacgaaaag gaaatcccga
    14881 accacgctat tttgatgttc ctttagggag tatcaattct atgggacttc caaacttagg
    14941 tttttcttat tatctagagt atgctcttgc gtatgaaaaa gtgcaggaaa accaaaatca
    15001 accgttattt ttttccattg ccgggatgag tgtccaggaa aacttggaaa tgcttgagaa
    15061 aatagaaaaa agtgggttta atgggatcac tgaattaaat ttatcttgtc ccaatgtgcc
    15121 tggaaaacca caactagctt acgattttga ggccacttat gaaacactga aagaagtatt
    15181 cagcatcttt tctaagccgc taggaatcaa attgcctcct tattttgatt ttgctcattt
    15241 tgatcagatg gctgatatct tgaatcagtt tccattgacc tatgtcaatg ctatcaacag
    15301 tgtgggcaat ggtttgtata tcgatacaga acaagaagca gtagtcatca aaccaaaaga
    15361 agggttcggc ggcattggcg gagagtatat caagccgact gctttggcga atgttcgcgc
    15421 tttttataca cgtttgaaac cagaaatcca aatcattggt acaggtggga ttcggacagg
    15481 acaagatgca tttgaacatc tgctatgtgg cgcaagcatg ttacagattg gtaccgagct
    15541 tcataaagaa gggccggaaa ttttttctag aatcataaaa gaattaacgc agattatgtc
    15601 agaaaaagga tatacgtcaa ttgacgaatt taaaggcaaa ttacgcacca tttcatagca
    15661 aagagacgaa ggtcatgatt gatcttcgtc tttttttata aaaaacactc gtctgttcat
    15721 tcctgtgcaa gcgtactcat tgtatgttag acgaacttgt gggaaaataa cagaggcaaa
    15781 aaggaggata atatgaatga tgcaacagaa ttagcaaaag agcttaaaga aaaagaattt
    15841 agttttcaag aactagttac ggatatacag aaaaaaataa agaagcaaaa tcatgaattg
    15901 aatgcgtttg tcgcgcttga accattcgag agtaattccc acgagctatt gattccccag
    15961 tcagaaaaag ggccattggc tggtatccca tttcctttaa aaatgctcgg tcaagaaaaa
    16021 aagggatggc tagctacttc aggatctcgt ttatttgaaa cacaccgagc ttctcataca
    16081 tctaattatg tgcggcaagc agaagcgata ggtctagttc cttttggaca aaccaatgcg
    16141 ccagagtttg gtttcaaaaa tattacagac ccagtcatct atggcccagc aagaaaccct
    16201 tggaaccttg aatgtacacc gggcggatca agtggaggag ctgctgctgc agtttcatca
    16261 ggaatcgttc ctttagctgg tgctagtgat ggcggtggtt ctattagaat acccgcttcg
    16321 ttttgcggat tgattgggct gaaaccttcg cgagggacga tgccagttgg tccatatgct
    16381 tggagaggat ggcaaggagc tgctattgat ttcggcttga cagtttctat gagagacacg
    16441 gaggctttat tttatgggat gagaagtatc cattccggtg cgccatatca agtatcgcca
    16501 gttgagtggc aaacacatcc aagaaaaaaa cggttgaaga tagctttttg tgttgattca
    16561 ccgatccact cacagattag tgaagaggca gtacttgctg taaaaaaagc tgtagatttc
    16621 cttggaaaag aagggcatga tttgacagag attccttacc cactagatgg tcgtaaactg
    16681 atcacgtctt attatcagat gaatggcgca gaaacagcag caatgatccg ctcgattgaa
    16741 acaggcatcc agcgagctgt cagaaaagaa gaacttgaac ccatttcttg gactttgttc
    16801 caatatggtc agaagctctc tgccgctact tacatcgatt ctctgcatgt atgggatgaa
    16861 gcagcaattg tcatggaaaa cctacatcaa gaatacgatc tattcctttc accaacgact
    16921 gcatttaccg cccctaaaat cagcgaagat ttgcaaagtg atgcgataag agcacagatg
    16981 gaacacatca gtgattgttc tgaagtagaa ggtttagacg ttgtttcttc catgtttgag
    17041 gagagcttga agatcacacc ttatacacag ttggctaatc tgactggaca accagcaatc
    17101 agcttgccta ctcatttgac aaaagaaaac atgcctctag gaatccaatt tatggctgcc
    17161 agaggaagag aagatttgct atttcaaatc ggctatctat ttgaagcaaa tcatcagttc
    17221 catttaccaa atccatggaa atagcagaaa caaggagaag aagaatgaag aaaaaaagat
    17281 tgtttgctct aataactagt gcctttttta ttttaagcgc ttgcggtaca gctgccgcta
    17341 atcaggaaga taacagctgg agtaatgtag aaaaaaagaa agaattagta gtagcgactt
    17401 ctggcacact gtttcccgca tcttattata atgatcaaaa tgaattagtt ggttatgatg
    17461 tagatttggc aaaagaggtg gctaaacggt taaatgtcaa aatctccttt aaagaataca
    17521 acgtggatgg acaaatcact tctgtttcaa aaagagaatc agattttgct gcgaacgatt
    17581 ttggtatcac aaaagaaaga gaaaaaaaat tctccctttc agagactttc aaatattcat
    17641 ttgacagtat gatcgtacga aaaaaagatg attcaggaat ccactcactt gaagatctta
    17701 aagggaaaaa agcagctggc gaacccaata ctagctatat gcgtttagcg gaaaaacacg
    17761 gagctaaatt agtcacatat gacaatgcca cgaatgatca gtatttaagc gatgtagcaa
    17821 atggtcgaac agatgttatt ctaaatgatt attatctgca gaaaatggcc gtatctgctt
    17881 taccagatat ccctgtaaaa atactggaga acgtttattt caatgaaaac gaaagtggtt
    17941 ttttattgaa agaaggaaat gatgctctga aaaagaaaat cgatggcgta ttaaaagaga
    18001 tgaaagaaga tgggacgaca aagaaaatat ctgaaaaata ttttcaaact gatgtttcag
    18061 tcaaaccgaa agaaacgatg gctgagtcgg tgagtgagtg atgtactttc ctctaattga
    18121 tttttccctc atgtggactt cacttccttt cgtactgcaa ggcttggtat acacattagg
    18181 gattggcttc gtgtcgtttc ttttaggaaa tctggtaggg ttgcttttga cagttttagg
    18241 tcttttggat tggctgcctc tgaatgtatt tattcgtttt tatttatcgt tctttagagg
    18301 cattcccgca ctagtcctgc tgtttctgtt atattttggg ttaccttatc aattaagtgc
    18361 cttaacggct tcagtcatct gctttacgat taccagcagt gcatttattg gtgaaatcta
    18421 tcgaggatca ctagcaggcg tttcttcagg tcagtgggac gctggtttag cttctggttt
    18481 ttctttcttt ggggtattga aatatatcgt tttgccgcaa gcttttcgaa tctcagtacc
    18541 ggcactcagt aacgtagcta tggatctagt gaaaggtact tcgttagcag caacaatcac
    18601 agtgccagaa atatttcaaa aagcaaagat cataggcggt cggacattcg attatttttc
    18661 tctctatatt ttagttgcac tgatttattg ggtcatttgt atcgggatcg aaaaagtgca
    18721 acagtattta gaaaaacgtt ctatgcatca gtcaatctga aaaatagaaa tggtgctagc
    18781 gtggctggca ccatttctat tttttgtttc ttgtatattc tgcctagtat tttaaaacca
    18841 tttcatgtaa aatgtaaatc gaaaatatgt tcggaggtag aaaacatgaa gaccccgcta
    18901 accatcgaaa tagaatcctc gttataccaa tattgcatcg agcaaggcgg cttggttgta
    18961 gaagaagtca cgatgcctga tgaccaagga attgtagata cgcttagctg cttgacaaaa
    19021 tttgatggaa gccgagaatg gcgatgctat gagctgaaag tgacaaaagc agattttcgc
    19081 tctacagcca aactgtcatt tgttggacat tataattatt ttgttttacc aagtaaacta
    19141 tatgaagaag taaaaactga aatccctaca ggaataggtg ttttagtgta tcgtccgtac
    19201 acggttgaag aggagatgcc tgcctctggt acatttctga ttgcgaaaaa agcagcaaag
    19261 aaagagcttg ctgtaccgga agaagctttg acaaaccgct tcatggcttc tttattcagg
    19321 gaagtaagaa aagcaaagca aatggagcga ggcgtaaaat attttccaac agatcaattg
    19381 tacaaagaat tgaaaagacg ttcagaaaat caagatccat tttctaacga acgatattat
    19441 gaacggtttt tagaggaaat agctagtcag gcaatcaatg agttgcaaga agaagttatc
    19501 gcgcttcagc atgattatga atatttgagg caacaacagc agatccgtcg gttgcctaca
    19561 gagccattgg agtgaaaaat atgtactatc cttatttaag aggaaaacaa tttgacctat
    19621 tagcattaaa agaagcactg tcgcgaggat tgctctccaa taaaatccaa ccagtgatcg
    19681 aaccagtacg tgattccgct acgcttaaaa atgtgatcga attatttcaa aaaaagcacc
    19741 atcgaatcgc tgtgatccaa aatccccaag taggacaatt caaattattc gatcagcatg
    19801 tgcacagttg gacagtcaaa gaaaatagct ctgtcgtccc tgcacagatc ttaacgccag
    19861 aaaatctcag tgaagtactg gattcacctc cagcgtttct tgttttcgat ggacagcact
    19921 acccaaaaga tacagaggtg tggaaacagt tggcaggagt tgattcgaaa tttttgatac
    19981 cagatactag tcgctttcgc atctggctgc ccgaaaacaa aattgtgatc cgtgactctt
    20041 ttcaaacgag aaagcatgtt gaaagttatg cagataaaac agatgacttt tttagtgatg
    20101 actatctttt tttccacgca gacggatata ttggattttc tgatttcacg atcgaaggta
    20161 gccgttattt cgataaaggg ttccctagcc gagcgcttgc tatccatcta acgtatattg
    20221 atgcttacgg caatatacgc gtgaaacatt ttgtctctga cagtaacgac tctgctaaag
    20281 accaagcatt gaagtttctt gaagcaggag ataaaatgaa aaaatggatc atgcgtcacc
    20341 atcatcaact cttgattact tcaggaatga ttgaattatt ggctctttac cagcaacaaa
    20401 aattccctgg actgggagtg ttgaaaaagt ggtcacttat gcatcatttg gaacttatta
    20461 gtcacctgct agatcatcca aaagattggc ttaaatatta ttcaaaagtt caggaactat
    20521 atgaagtgat ccaatgatta gataatgtaa atgaaaaagg aatagaaaag aggaaactgg
    20581 atgtatattc ggcaaacaag gaaagaagaa gtagaagaag tggtagaaat cattaatgcc
    20641 gcagcggatt tcattgcttc gcaaggctct cctcaatggc aaaacgggca gaaaccaaca
    20701 aaagaaacga ttttgaacga tattgaaaga aaagaaagct atgtattaat agtggaagat
    20761 caaattgctg gaacagctgc attagtctca agcgtggatc ccgtttacac aaaaatagat
    20821 ggtagttgga aagaatcaac tggtccttat ttatcgattc atcgtgtcgc tttagcagaa
    20881 agtactcgag gaaaaaaact agcaaaaccg ttcttagaac atttgttgac gattgcagcc
    20941 agcaaaaata tccaagatat ccgtatcgat acttatccag ctaatgaacc gatggaaaag
    21001 accatctatt ctgctggatt cacttatcaa gggatgattg aatttccttt tgaacacggc
    21061 gaaagaaaag catatcaaat aatcatcaat tgactataaa atcatctgag caaagataaa
    21121 gacattaaca ctactgaaaa aaacaatcat atgatagcca agaggttgta acagaagtgg
    21181 ccagcttcaa aaaacaagaa agaatttccg aaaatgcttc tcaaattttt gtggaatttt
    21241 agcgtatttt acgaaatagc tgctttctga aactcccttc atcgagaggt cgtgacatag
    21301 cttttgtcac gacctctcgg cttatcctac gagtattatt cgatacattt atatcaataa
    21361 tccttttttt attttatctg cagcttcttg gaacgctcca tcgtttgaca cgactaaaag
    21421 tgaggggata gctttcaaac gtttcaactg tgctaaatct tctacataaa ccgccagtct
    21481 ggctgaaatc tgttcagatg aatctccacg ttttttcagg cgcgattgca atgtttcttt
    21541 agagatcgta ataaagatag gacggactac cgtcccgaaa tactcatata aatgccaaaa
    21601 accatttgct gtcaaaacgg cgtaacaatt tcctactgtc agtttctttt taatttctgc
    21661 tttactggaa ccatatttat gttgagcata ttggtcccac tctgcaaact catttccctt
    21721 gatcatctga ttgaatgtat tgtcagaaac aaaataataa tcctcttgat ctttttcccc
    21781 aggtcttggt gatcgattcg tgtaagtaat gatcttttgt tccggctgaa aaatagcttc
    21841 tgcgagtgaa gtttttccag aaccagaagg accgataaat acatagcaat aattcattgt
    21901 catttctcct ttgatgattg aacaagtgaa aaagctttgg aaaatatttc caaagctttt
    21961 aaaagtaact ttgctttttt gtatattata ccagcaagcc agtcacaata gcacttaaaa
    22021 agctgactaa agtcgctgtt agcaaaattt tcaatccgtg ttttgcgaca agatttcctt
    22081 tttcttcatt taatcccttc atcgcaccag aaatgatccc gatagaagag aagttggcaa
    22141 atgaaacgag gaagacagat ataattgctg ttgtacgctc agtaaaatgg aaattaccat
    22201 ttgctaattc tgtcatagca acaaattcat ttgtaaccag tttagtcgcc atgatactac
    22261 cggcatccac tgcttctttc catgggattc cgctgatgaa ggcaagaggg gcaaagatgt
    22321 aacctaggat ctcttggaaa ggatgccgaa tatcccgtgg aagatagcat tgatcatggc
    22381 aatcaaagca acaaaaccga taagcatagc agcaaccgtg atcgccacat ggaaaccatc
    22441 aagaatgtat tctcccaaca tttggaaaaa tgtctgttct ttgttttctt caatgatcag
    22501 ctcatcttct ttttcttcta atacataggg atttacaatt gaagccagaa tataaccgcc
    22561 aaataagttc aaaacaaggg cagtgatcac atatttagaa tcgatcagtg ccatataaga
    22621 tccgacgatc gacatagaaa ctgtagacat agccgataca ctcatggtga acaaacgttt
    22681 ctcagatagg aagggtaatt ccttttttat ggaaataaaa acttccgatt gtcctaaaat
    22741 agcagaagct actccattat aagattccaa tttgcccatc ccgttgattt tacttaatcc
    22801 tagaccaaca gctttcataa ataatggaag aatcttgata tagcgtagaa taccgataat
    22861 agcagaaata aatacaattg gcatcaatac ggaaagaaag aaaggtgcac ttcctttatt
    22921 tgctacaccg gcaaaaacaa agtcaacacc ttgcccagca aaagcaagaa gatgatcaaa
    22981 catttttgcc aatacagaaa caaccgctgt accaaaagtt gtacgtaata atacgaaacc
    23041 aagaacaagc tgtaaaacga acatcacaat tagtggtttc aatttgattg cttttttgtt
    23101 actgctgatc aaaaaagcaa gccctaatgt gacaaataaa ccaataatac ctaataaata
    23161 actcaaataa tcactcctat tccaaattcc gttttatttt acctgttttg caaataaaat
    23221 actaatcttt agtgaaattt tcattacttt tttagagtga aacgcattca ttttccatta
    23281 gaaaataaag cgaacaaaat aaaaggttta aaaagtgtaa cgtcttagta aagtctataa
    23341 aaaaacgaaa ctatcgatta agctaatagt ttcgttttca acatttataa gtaaactttt
    23401 tctattggta atatagattt tcggaagggt agacaggtgc gcaggtaata tcgattccta
    23461 gttttttcaa tgtttgttca tcatttttat tgaccatcac agtagagtgg gcttgtgctc
    23521 cctcaagttc agctagttta tcataagcaa cttgagcgat tggattcgtt actgcactga
    23581 ttgataaagc gatcaagact tcattgatgc ttagtgcatc caaagaggaa tgaagtgtct
    23641 ttgatttcaa tccttgtatt gtttcaagaa ctaatggtga caataaagga atgtcatctg
    23701 cgatatgggc taaatgtttt actgcattta agataactgc cgctgacgca tccatcaatg
    23761 aacttgtacg cccggtaatg atgacttcat ctggtaattc gattgcaatc acagcagaag
    23821 gatcttttgt aggactttgc gcttgaatct tatctgcata gtcatgggca gggactacca
    23881 cgtttcggtc ttcaggacgt aaaccgactt cttccataat cagtttgatt cgattaactg
    23941 cttcttcgtc gatcaatcct tttttgaagt cgcattctgt ttggaaataa cgacggatga
    24001 tttcttgttt agaggcttct tggacaacgg tatcatccac gatcccaaat cctacacgat
    24061 tcacacccat gtcagtagga gattgataaa cagattcttt accagtaatt ttttcaataa
    24121 tacgtttgat tacaggaaat gtctcaacat cccgattata gttcacagct actttattgt
    24181 aggcatcaaa gtggaaggaa tcgatcatat tgacatcttt tagatcgact gtggctgctt
    24241 cataagcaat gttcagcgga tgtttcaacg gaacattcca tactgggaaa gtttcgaact
    24301 tggagtaacc agctgcatgt cctttttggc tttcatgata cagttggttc aaggaagtag
    24361 cgagctttcc acttcctggg ccaggagctg tcactacaac gattggttta gttgtttcaa
    24421 tatactcatt ttttccaaaa ccatcttctg agacgatttt ttctacgttg actggatagt
    24481 cttcgatttc tgagtgttta tagaccttga tgtttcgtct ttctagttta ttgataaaga
    24541 ctttcgtggc aggctgtcca ctatagcgag tgataacgac actgttgatt aataagccat
    24601 aaccacgtaa ttcatcgatt aaccgcaaga tatccatatc ataagtaatc ccataatcac
    24661 cacggatctt attacgttca atatctccag catagacgca aatcagtatt tcagcttgat
    24721 ctttcaattt ttgcagcaat ttgattttcg catcttcttc aaatcctgga agtacacgtt
    24781 tcgcatgctt gtcgtcaact agcttcccac caaattctaa atataatttg tcatagtggt
    24841 tcactcgttc caaaatatac gctgactgct cttcaatata tttttctgaa tcaaaaccaa
    24901 tttttttcac taggttccct ccttaagtac taaagcattt tttcattttt ttgaggattc
    24961 gtcaactgga agagcgaaat tcctcaaaaa aaacaaagaa aatggtttaa accaacttgt
    25021 ctagatagtt attcattggt atgatagttt ttgataagga aggtgctaca tgaaaaaaag
    25081 acagaaaaag aaaaacgcat ataaacacta tatccgttcg atttttaccg gttatgaaag
    25141 aatgcttgaa gatcccgaat tggaacaatt gacgtttact tatctaaacg aagaaacgca
    25201 gctaactcgt gatgagcatc agcgcattca tttcacaaca agagatctgc ctagtaaata
    25261 aaaaataaga taaaggtcat cggattcgat tctgtcctat cagatggaaa gactagttga
    25321 tcgaaaaaaa ggctgtacca aattttggtg cagcctgttt tatggagcat tgcccgctct
    25381 tttttataac aattttttac agaaaatggt actattgaag tattaataag tgaaaaacag
    25441 atgagtaaga ggaggcaagt agtatgaaag agccagatac aaatgcaaat tatgagccag
    25501 acgaactaga ggagacgaat gcggatcagg atacaggaca agaatcgtct tctttttacg
    25561 atatcaaaga aaaagcaagc atgaatcttt cccaattatt agaagactta caggcttttg
    25621 aaaaagctgt ccgcgaagaa gatatccctg agatctaccg tttatacaat ggccgattga
    25681 atgaagaatt aaaaagaagc tcaaatgaaa atcacgagat cgatcaattg ctgatgcgta
    25741 agatccatga tcgtttttta caggaatttc cttttatgga acaagtagaa aacatctcgc
    25801 caacgatgag ctattacaaa atcggcactt attaccatga tcggcccacg gtcggtattg
    25861 atgcaagtct gccagaaatt tttgttttgc ctaagatcga tgaagagtgg gagaaatatt
    25921 cccaaccgga gtcaacggac tatgacaaaa aaatcaatga gctagacgca aaggtgatca
    25981 ctgctcagac agaaattgaa cgtttagatg aacaaataaa agaaatcaat cgacaaatga
    26041 ctgatttgaa cgacaataaa ggattcttga atcgtaaaaa aatcgaggaa gaaattcaag
    26101 aattagagaa gaagaagcaa gtactatcta atgaaaaatt aggctggctt ccttatattg
    26161 agacgcctga aacaatccag cagcaaaaag aagcattgaa gcaagaagca agagcagacc
    26221 agcttcgtgc agccatcgta gaaaaagaac aaaggcaaat cagacgctat ttcggtagca
    26281 aagagggatt cggtcaagca atccatgaat tcttgatgaa ttatcttggc aatgagaatc
    26341 ctaataatca atctaatgaa ggaggttccg agtatgaatg aacacgatga agcagaatgg
    26401 acaaaaaatg aagaacaaac agaagatcat gaagaaacga acacttcatt ggaagaacaa
    26461 acaagtaatc ctctggatga cacagaagaa atccctatgc tagaggaaac aaaaatacac
    26521 gaaaaagaaa aagtaactcc gacgaatacg aatactgcag aaaacaccca tgcttacgaa
    26581 ataactgaaa atcccgggta tttagaagga aaagagcaag atcatcaaga tatcgtcagt
    26641 ttgattgaaa cagcggaagc agaattagcg gatttacgta tgagaaaaga tttgatcgaa
    26701 tcagattttc cacaattatt agaatcatat gatcgtttta tattgaatga agaaccaatc
    26761 tccacgatcg gaaagacaag cctgatcgaa tacttcacgt atgaattact gaaaccgttg
    26821 aatgatattg gtctggaacg gtcaaattca gcttatgaca catggaaaac aagacatttt
    26881 tcgatcaatt atacgttgaa tggacaaaac gaattagtct tttcctttgt attaccgacc
    26941 atcgatcaac gataccaagt ggaatcgatg agtctcctaa acgtatcacc agagacaatg
    27001 gaaatcaatg tccaagatga ccgggtgctt tctttgatcc gacactggtc agtggatcga
    27061 gtattctctg cagggcagat cacgatattc aatcacaaat tgaatcaatt attagcccat
    27121 gcaagaacac ttggttttac cgttaatcag acattactag acaatacgaa gccacttcgt
    27181 ttgaatcttc agagcgaatt tgaattgacg aatgaagtat tggatgatat ttttatcgtg
    27241 gcaatgaaaa atccatccta cgatatggaa aagatcaacg aaactactta tcaagtacga
    27301 ttagataaag gacagtcttt aacgataaca acaaacgaaa aagatcaaac agtcttgtcg
    27361 atctcatctg gagactaccc acgatctgtc attgatttct ttatcaatta cgaattcttg
    27421 gtcccattaa tggtgagaaa gtcctgattg gataagttgg attaccagat gtcattcaga
    27481 taattctgtt ccaaaggact tgcggcagtt atccagaata attgtcacaa gtcttttctg
    27541 tatgtactaa atggacgtga ataacttgct gatgaaggag ggatttcatt aggaatgtcc
    27601 acaaataaaa tagcacagct tagcttattg tctgctgctt gtgtagcagg taggatcgct
    27661 tttcaattta ttccgaattt ccagccaatg actgctattt ttttatttat agtactttac
    27721 ttaagcttaa aagatgcgtt aattgtcatg agtctttcta ttgcgatcag cagtttttat
    27781 tttggtgttg gaccatgggt catcggccaa tggatcagct atactgttgt tctgagtttg
    27841 ttttcaatcg tctgcttgag aaaaacagtg caaaggaatc tatggttaaa gggagtctgt
    27901 ttttttctgg cgggcatggt ttacggaata ggtatgaccg tttttgatac gctgttatat
    27961 cgtttgcctc aaccttggat ctattacctt caaggggtgt cgtttgattt gatgcacagt
    28021 ataggcaatg ttgtgttctt tcttgttttt ctgccagtcg tcaaacgttt ttacaatcat
    28081 tcaggaggaa aaaatgaaaa aaataattta tagtttgctt gcaatgagtg tcagtatact
    28141 cattttgaca agttgcagta caacctatac acaatcggct tccaaccaaa aggaggaagt
    28201 aaaaacatcg caaaaagcaa caatcacgtt acaagaaaat ggtcaaaact tttcggaaaa
    28261 agaagtgttc ttcaaaaaag gcgatgattt actgagtatt ttgaaacgaa attttgaagt
    28321 caaagaagat aacggcttca tcacttcgct tgaagggcac tcacaggatg agaaaaatca
    28381 actctattgg atgtttactg tggacgggaa aagtgctact actggtgcca aagagatcaa
    28441 attgaatgat ggacaatcgg tcattttcaa tttgtctaaa ctataagttt cataaaaaac
    28501 tgttaaaggg cgaatattgc cataattcag tgagttcagt tgatgaaaga aggaaaataa
    28561 catggaaaat tttaaaaaaa gcggaatgtt ttccgtaatt gctgctctag gggctaatat
    28621 tctagttgct atcagcaaat ttgtcggcta tgcaatcagt ggcagtgccg ccatgctaaa
    28681 cgaaagcatc cacagtatcg ttgattgcag caatcagatt ttgctgttat tcggtgataa
    28741 acaagccaat aaaggacaga gcgagttgca tcaatttggt gaaggacgag caaaatactt
    28801 tttcagtaca atcgtcgcaa tgatgctttt cttcggcggt ggggcacttg gggtgatgga
    28861 agcttttgaa aagttgacgc atccatccca tgaagtgggt aatccgatca ttgttatagc
    28921 aatactgtta tttggtttgg taatcgaagg aagttcgtta cgcgtggcaa tgaaagaaat
    28981 caaagaattg aatactgaaa aactttccac gatgcgcttt ttgcgcgaaa gtcgacacag
    29041 tgaaatactg attattttta cggaagactt gtgtgctgta atcggtttgg tcttggcttt
    29101 agcaggaaca atattgactt atgcaaccgg gattgctgca tttgatgcga tcagcggatt
    29161 gctgatcggt ttcttgctga tgggagccgc cattttccta gcaaaagaat tttatagtct
    29221 gatcatcggt gaaagcgtga cagcaagcga tttatcaaaa atcaagttag cttttgaacg
    29281 tcctgagatc agtcggctga tcgatataaa aacagttcat ttaagtccaa cggagatttt
    29341 agttgcagca aaagcagata tcgttggtaa caaagagaat gaagcttact cggtcatcaa
    29401 tgatattgaa aaaaatatgc gtcagtctct tcctgacaag aagatgtata tatacattga
    29461 aacagataaa tatgacccaa attattctcg taaataaaac gtttattcat gaattgaagc
    29521 aaaaatgaga aaaatataat cttgttttta aataaaacgg ctacaattta gcgaatttat
    29581 gttacaatga gtccgctgtt tatttgttgt aattaaagga gatattttaa tgaacaaaaa
    29641 gaaaagtgcg ccatggcttc aagctttgca tgcagtgatg ccactttgta taagctatat
    29701 accagtagga ttagcttgcg gagtattgtt gcaaaaagtt ggatttgatc ctctcttatc
    29761 aggactgtta tcgattctgg tcttttccgg tggcgcgcag tttttagttg catctatgct
    29821 tacgacgcaa gcatcatttg caacgacatt attgatggtt ttctttttag aattacgcta
    29881 tacattatta ggagcaagtc ttgccggttt tatgaaaaaa gaaaagcgga cgtttttggc
    29941 tgtcttttct caatcactga atgatgaaaa ctatgcggtg aactacttaa agttttcaac
    30001 tgacccttcc tggaataaaa gaaaggcttt atatgtcaat tggttctcaa tgactgcatg
    30061 ggccggtagc aatatgatcg gtaatttttt cggctcggtg attcgagtag atgctgacct
    30121 tgtccatttt gctttgacgg ccatgttcat tttcatgttt atcatgcaga tgaaaagcgc
    30181 actactgatc tttacaggtt tattctcagg cgtattaggg gtcgtattca tggtattatt
    30241 ccaaaatact ttcggattga ttgcagcaac agtcattgct tcatttgccg gatttatgct
    30301 ggagaaggca ttcaaaaaag aaaaagctaa gaaactaaga gaccgcggta aagatgtcaa
    30361 tgagccgctc ttcaacttcc cggaacagga ggccaatcac catgactaaa ctatatttgc
    30421 tacttttagt tgcttgtctg ttcatcgtgg catatatccc gcgcttgttc ccgatgcttt
    30481 attttactca tcgaaaagtg ccatcctggt tcagtgactg gatgaaatat gtaccggtgg
    30541 cattatttgc ggcgttagct tttaaagatg ttttcattac tcatgaacat ctggatattg
    30601 cttggaatat caaaatagct gcaatgattc tagtagcagg cgtggcttac aaaacacgtt
    30661 cgatggcgtt gtcagttttg acaggacttg cgtctgtctt tttgctgtct atgctttaaa
    30721 ctgttttatt ttattaaaaa cagagattgc gacataagtt gttcaaactg atctatccga
    30781 actgcatttg caacagtcaa ttctggatgc aagtttattc gtagggataa aaaagattag
    30841 tgttggctta tgatgcaagc tcttttttat tttaggaatt agagagggat ttgtccttct
    30901 atgatttttt gctataatag ggaagatgtt tattttatga aagaatagtt gagggaattt
    30961 aaatgaataa aaaattgatg caaggaacat tttggctaac ctttgctaat cttctatgta
    31021 aagttttagg ggtagtctat ttgattccct ggctaagtat gatggggaac aatcaagatg
    31081 gtatgcttgc tacgacattg tataatgttg gctatcttcc ttatggattg ttcctgatgc
    31141 ttggtacagt cggatttcca aatgcgattg caaaaaaagt agctgtagcc acaaaagaag
    31201 gagataacgc tgcttgtcgg ctgattttta gaagtacgat caatatcatg tttgtcatcg
    31261 gtatcgcctc tgctgcactg atgtatttat ttgctccttt actagccgga atcagtccga
    31321 ttgctaacgt taataatggg atactagcta tcagaagttt atgcccatct ttgattgcta
    31381 tcccaattct tagtgcaatg agaggatatt tccaaggaaa aaatgatctg cggccttatg
    31441 gaacttcttt gattatcgaa caggctgtgc gagtgatcgt tatcttggct ggtacttatt
    31501 acttgcgcgt attgactgac ggaaccatat tagaagctgt attgatcagt acagtcgctt
    31561 ctttcttcgg tgggttagct gcgattatcc atatgtatat cgtcggacgc agaaaagact
    31621 tcttcgaact taaagatttt ttgatttcca gccgttattt cgaaagagaa aaccgatctg
    31681 cctctgtctc tatcattcgg gaaacactac cgtttatttt tgtcggctca gtcattacta
    31741 tcgttcaaat gatcgaccaa gtaacgatga aaccactgct tcactttttc cgtccagaga
    31801 tcgcagatca gcaactggaa ttcttattca gccgtgcttc tgtcaatcca aataaattga
    31861 cattgatcct gatctccatg gtaggaacag tagctataag cagcttgcca atattaagca
    31921 cgatgaagaa aaaagatcgg atacaaatcg aaaaaacagt aggagatagt ttttcgattg
    31981 cgttattgat tcttcttcct tcattgactg gaatgtcttt attagctgga ccactataca
    32041 cattgttttt tggctatgat cctgaaagtg tcggatattt ccaaatggct ttgctagctt
    32101 ctttgttttt ctctttattc acgatcctat ctacgatgct tcaatctttg aatcaccatc
    32161 gttttgcgat ccgattaact gtagaagcaa ttatcctgaa agtcatcttc caagtaatcg
    32221 gattaggatt agttggcgga tacgggatga gcttatcaaa tggcttagca ttcggtattg
    32281 tctttgttcg cggctatcac tttatgtgca aagaataccg aatagcacct ttagcaaaga
    32341 tcagcaattt tttcttaaaa actttccgct ccaccctgat tatgcttgca gtatgttttg
    32401 ccgttttctt tttgctgaat cagcgattgt caatggaatc aaaaagctat gcaatgattt
    32461 attgtgtggc tgtaggaagt attggcggct tagtattttt attttctcaa ttcggaaaaa
    32521 atgggtttcg aatgttgaaa aatgttaaac atcatagcag aagcaaagaa taggttaact
    32581 aatcgttttt tgctgtcact tgaaaaacgg caactcgtat gattacgctt gtcgtttttc
    32641 tattttccaa ttttagtttc atatctaatc ttacttatga tatagttata aggtctggaa
    32701 aaattctttg aaacgaggaa attaaacaca tgaaacttgc agtagttaca gatagttcgg
    32761 catatctgcc ggaacgtatc aagaaccatc ccgacttatt cgttattcca atccctttga
    32821 ttatggatgg gaaaatttat aacgaaggaa tcgatatcga agcggatgaa tattatggac
    32881 ttttaaaaaa cagtaaagat tttcctacta cttcacaacc tgtcgtaggg gaagttttgg
    32941 gtttatatga ggaattgaaa gagaaagggt atgacacgat tttaagtatc catctttctt
    33001 cagggatttc tggctttgtc aacacattat ttgcgatgaa agatgacatc agcggagtaa
    33061 cggtcattcc ttatgactct aagatcacta gtatgccgat gggacacatg gtagaagctg
    33121 ccttagactt aaatgatgaa ggtcagtccc ttgatgaaat acttgcacat atcgatcgaa
    33181 tccgagataa tacctacgct tatctgattg tagatgattt gaacaattta gtacgaggag
    33241 gacgtttgac aaatggtgca gcgctgattg gcagcttatt aaaaatcaaa ccgatcctga
    33301 cattttctga agggaaaatt gttttattcg aaaaaatccg ttcaagtaaa aaagcatttg
    33361 ctcgagctga agaaattatt ggtagaagaa ataacgaaat acaaccgcca gtcaaacttt
    33421 acgtgataca tgcaaataat ttagaagtag cgattgcgga gaaagagaaa ttgcagaaaa
    33481 aatatccaaa tgcagaaatc gaaattggtc atttcggacc agtcattggt acacatttag
    33541 gtgaaaaagc catcggcatt gccatttcag cgcaataatt ccttttattc aaaaaacata
    33601 ccaaaaatat tccaacatca tcttggactt tctacatgtt actaatgagc gtgtaaaaga
    33661 acgagatggt gtttttattt ctatcaatga agctcttttt ctttgcaatc ctcacttcaa
    33721 tcctctataa tgtcgcatat agtttagtaa atgagtaaac tactaattta cttatttact
    33781 aaaaataaaa ggatgaggag ttaaaataat ggaaatacca acaaatttaa aacataaacc
    33841 agtaatcgtt gtagaagact atgataaagt tgatggaaga aatgcattaa atacggatgc
    33901 aaaagggctt tcactgggcc ttgctcagtg gaatgatcgt ggaaaagtcg atatctcagg
    33961 caaggtatgg cgacatactg gagaaaaatg gtcacgacag tcggaagaat taccaatcac
    34021 acgtatttta gatttagcga tcttagctgc acaaggaagt ttatacttcc aagatgctta
    34081 tcgttatgaa aaattttatg atccagaaaa tccagtcgta gacatcattg gattacaagg
    34141 gaaccgaatg acggtagaag tagataccaa aaatccaaag atcgatgaag acatcatttt
    34201 gtattacgat acactacaaa aagacggaga actgattggg gaacgattcc gtattttaaa
    34261 acgtctttta gatgagctag gatattaata gacagagatg atcaaaaggg gaagagaagc
    34321 atggagaatt tctcactaac agaaaaccga accttttcac ggctagaaaa gcaaatgatc
    34381 tggaagaaac cgaaaagcca tcggacaagt gcagaagaat ggcggatcgc ctccgaaatc
    34441 aaagggaatt ggcaagatcc tgaaatgaaa atcagtaacg tacttttgga aggagatgcc
    34501 gggtcgggga agacacaatt agccaaagca ctatctgctg atctgcaact tccctataca
    34561 aaagtgacct gttttgcaga tatggacaaa tcagatgttt tcggagcctt attacctgta
    34621 gtagattcta ataaggaaca agaccaagaa ttactagaag ctatttacca aacagataca
    34681 cttgaagctg tactgacact gatccagaac cattacggca ttgatccttt ttctgctaaa
    34741 gaaaaattag cagagctagt tcaaaggatc gaagctgatt atccagatgc aatccattac
    34801 agctattacc cttctgaaat cgttcgagcg attgaaaaag gatatttatt agagattcaa
    34861 gagccgactg tcatccgaga tgcctctgtt cttgtggcac ttaactcagc gctcgaacca
    34921 aatgggatgt tgaatttacc gacaggaatc attcgtcgcc atccagattg cgtcattgtg
    34981 atcactacaa atcgaaatta ccaaggaaat cgcccattaa atgaatcttt aagagatcgg
    35041 atgcaacatg cggaaaaaat ggatcttcct gccttagaag tcatggcaga acgtgcgatg
    35101 gcgaaaacta tgatccaaaa acaagaactt ctcttaaaga tggcagagat tatccgtttg
    35161 ttggatgaaa ctgcaaaagc taacgccatc aaaggtgtag caggaatgcg ttcttatttt
    35221 tattgggcaa atacctataa gcaaggacaa gatttactcc agtcgattta tccaaaagtt
    35281 ttgtataaat tatccactga ttcagatgaa ttggcaattc ttgaacaagc tttgtctgac
    35341 agtggattgc tactggaact tcaggacata ttgagaaaag aaaaatgggg tcatcctcaa
    35401 tctgagcata caaaaggaag aaccattagc gccgaagaag caaatgaacg aaacatcact
    35461 gaaaatgctg aagaaattct gcgcttagat gaaaagaaat cgttgtctgc tgaagaacca
    35521 aatgagggag aagagaaaca agaagaaaca atagaaaaaa acgagattcc tcctcaaatg
    35581 caaaaagaac gcagtagcga tatcgaagga aaaaatcaaa aagaacaaca aacaggagat
    35641 gattctcaag aaatgtctgc ttctgacatg gaagcacatg ataaggaaat caagaagcaa
    35701 ctgaataaag aagcgcgaca aatcatgaag gaaacgattc atgaaaaaga aggtctcatc
    35761 gttcaccgac cagctttcca aggaaaaacg ccggaagcac gtgagctaca catgcaagta
    35821 atgccaatag tagaatcact tagccggcaa attttagagt tactggataa tgaacaaaca
    35881 gagacgtacc aaaaaggaaa atacgaaggt cagcggttca atgcaagcaa agtagcatat
    35941 ggtgatttga gaaattttga caaaaagaac ccgccacatg agcaaccttc tttagcggta
    36001 gctgtacgga tcgatgaatc tgggtcaatg atacgggatg atcggatcga agcggcaaaa
    36061 aaagcagcga ttgccattgc cgaattttct aagaaagtcg atatcccatt gttgatttac
    36121 ggagatacag cagaccgttc gtcaagagaa aagacttctt tgttttctta taaagaattt
    36181 gaagacggat ttcaatggtt aaatgaaaaa ttagtcacta tgaaaccaag gcaaaacaat
    36241 cgtgatggtg ctgcacttcg tttagcagca gaaaaattaa acagacaatc cgcaacaacc
    36301 aaactgttgc ttaatattag tgacggtcag ccaaaagcgt tgccggatta tacaggcaaa
    36361 aaagcaaaag aagatatcca gtctgttttg aaagaatatg aacgtcaagg aatcctattt
    36421 gtctccgcag ctattggcca agacaaagaa gaaatcaagt cgatctacgg tgaaagtcgt
    36481 tttgtagata ttactgactt gaatgaattt cccaagcaaa tgattcaact gattgcccgc
    36541 tatctatgat aaaataaagc tcaacgaacg cacttagcag attcgttgag cttttcgttt
    36601 ctagttaccg gtaacaagga tacttctaaa atagaatcgg ttataatcaa gagggaccgt
    36661 taaaggagtg aaacgatatg atacaagaac aaaaatggtg gaaagaagca gtaggctatc
    36721 aaatctatcc tgctagtttc aaagatagca atggcgatgg tcaaggagac atcaatggga
    36781 tccgtgaaaa acttaattat ttgaaagatc ttggtatcgg ctttatctgg atcaatccga
    36841 tctatcaatc tccatttgtc gataatggtt atgacgtaag tgactatcaa gatatagaag
    36901 agagattcgg aacgatggaa gattttgatt tattgctaaa agaagcacat gaattaggga
    36961 tcaaagtgat tatggatctt gtcatcaacc atagttcgga tcagcatcaa tggtttgaag
    37021 aatcgaaaaa atctaaagaa agcccgtatc gcgattatta catttgggtc gacggggtag
    37081 atggcaaaga accgaataat tggacatcga ttttcggcgg ttctgcttgg gagtattctc
    37141 atgaaactgg acagtattat ctgcatgttt ttgcaaaaga acaaccagac ttaaattggg
    37201 aaagcgagaa attgaaagaa gaactattca atatgatccg atggtggttg gataaaggga
    37261 tagatggatt tcgtttagat gcgatttctc atgtaaaaaa agatgaatat tcagtaaaag
    37321 ccacagagaa ccctttctca ccctttcaaa atgtctcagg aatcgaagaa catttgaccg
    37381 atttgaaaca tgtttttgaa gagtacgata tcatgactgt cggagaagca agtggtgtca
    37441 ctgcagaaga aggaccacaa tgggtaggga aagacggtta tttcgatatg atattcgaat
    37501 ttgatcatat ccatatctgg cagcaagaaa aagaagggca attagatgtc cttaaattga
    37561 aacttgcatt aagcgcttgg caaacttcat tggatggtat cggatggaac gctttatata
    37621 tggaaaacca tgatgttcct cgtgccgttt cagtgtttgg agatactcgt cccgattttt
    37681 gggctatgtc tgcaaaagca atcgctatga tgtatttctt tttgcaaggt acgccattta
    37741 tttatcaagg acaggaaatc ggaatgacga atatgccctt tgaatcgatc gatcaagttg
    37801 atgcagtaga ttcgaaaaga ctttataaaa ggctgcttgc ggaaggtaag acaagagaag
    37861 aagcattgga tatcattcgt gaaacaacga gagacaacag caggacaccc atgcaatgga
    37921 cttcggaaca atatgctggt ttttctacac atgaaccttg gctgatcact aatccaaata
    37981 ccaaaacgat caatgtagaa caacaagaat atgaaccaga gtcggtcctg caattctata
    38041 agaacatgat ccgcattaga caaaccaata aaggtctgat atacggttct tataaagaat
    38101 atcttcatga acatccacaa ctttatgtct atgaaagata tttagaagat gaacaatatc
    38161 ttattatggt aaacttaaca gaatcacttg ctgactatga acttccaaaa gaagcagatc
    38221 agtcctggac attactcttg tcaaattcct ctagcggaga atttgaagct aaaggcatac
    38281 tagcacctta tgaagctcgg ttatataaaa caaacaagta aaacttcctc gttaaagttc
    38341 attagttgga ctttgcgagg aagtttttca gattgctctg tcaattactc atttcaataa
    38401 ttcgctctag gaccaccgat atatttagga cgagagcgaa gggctgtcac gtgagcttgt
    38461 ccaagggttt tcaaacgagg tctgacaggt acttggacat gtttgatatg cataccgatt
    38521 gatgtgtcac cgatatcgat accggcttgt ccaactacgt gttccacttc gacaggatca
    38581 ttgaactggt caaaagcagc aacagaacaa gcacctccag catgtagtgc aggaacaaca
    38641 gagacaattt cgaaattttt tgcttctgcc actgttcgtt ctactactaa tgctcggttc
    38701 aaatgttcac aaccttggac agctagcgca atttctttag gatccaacaa ctcttttaac
    38761 gtacggatga tccattgacc aacttccgca ctggaatttt ttccaatatg tcctccaacg
    38821 acttcgcttg tgctgcatcc taaaacaaaa agatcacctt ttttcaagtg tgcctcagcc
    38881 aatatgtcat taactatttc agtcaattgg tcttttaatt ctttttctga caacttcatt
    38941 ttctaaaacc ttctttttca attaatattg cttccgtgct tgaatacgtt gaagacttgc
    39001 agttatagga aaggcaagtc ccattccaat cagattttgg aagatgtttc ctggtataga
    39061 agccactcca gccggccaat catataaaat ccaaccggcg ataccatagc cgatgatcat
    39121 aactaagctt cctaataaaa gccctaatcc ccacatacga gtagaagatt tgctgaaaga
    39181 accagcaaca tatccttgta acccgtggat cactaatgaa aaaactgccc actgcggata
    39241 tcctgaaatc aaatcgatca atagaccact ggaagcacca acaactaatc cacccgttgg
    39301 accataaaga aaagcagcgg tatagatccc ggcctcgcat aatgtcacaa atccattcgt
    39361 agccggtaca gggatcaaaa ttgttaatga aagggcaact gtcaacgcaa tcagcatagc
    39421 atagatcgtt aattgcttag ttttcatttc tcattcctcc tttttgaata ccgccagacc
    39481 agacacttcc ggtatgatcg ttcatcagca cgccattaga aatacaatgg gcaacaaatt
    39541 ttttactttc tgctactgct ttggtaaaat ctgcccctaa cacaagattc gctgttattg
    39601 cagaagaaaa ggcacaaccg gccccgttta ctgttgtagt gtcgattttt ttatgagaaa
    39661 atacctcata atgatcaatt gaatagagaa gatccacagc ttcatctcca gccatccttg
    39721 tgccgccttt gatcacaatg ttttttgttc ctgaagcagt gagttcttta gcgagatgga
    39781 tcatttctgt taacgaagag ggaagagctt gaccagaaag taaagcagct tctgccaaat
    39841 taggcgtaat catcgtaaca agtggaaaaa gttcttcgat gagctgcgaa atatacaatt
    39901 gattgatcac agcttcagtt tctttgaatg ccaaaacagg gtctaaaatg attgggatat
    39961 tttgttttgt ttttatgaag tctcgaacaa ccatgattgc ttcacttgaa ttcaataacc
    40021 caattttgat accatctaat gaagtcattt tatcaatcga aactaactgt cgtttaagca
    40081 attccaaaga tatagattcc agcataaatc gctcattaat atctagtgcc cctatagaag
    40141 tcaaggcgct tagtccgaat agaccaaaat tctcaaaagt ttttaaatct gtttggattc
    40201 ctccaccagc aaatggatca gaaccgccaa ttgtcaagat tcttttcata acattttcct
    40261 ccatgaataa gagtataacg aaattttcaa aaaagaaaaa cagccaattg gcatattttt
    40321 atctcatcca atttaaaaaa acaaaaagaa aagaaagacc aaaatggatc tttctttcct
    40381 taaatagtta ggacttttgt gattttaatt ctttctcgat ttgaagccgt tgcactttca
    40441 ttgtagctgt tcgcgggatt gcatcaaaat cacggatgat agggacgttc aagcgaggaa
    40501 gttcatggac ttgctcccac catcgattcc aatccatttc tttatcagga gctaaagcaa
    40561 taatcggttg tggagaatga tttttatccc ttacaatcac tacttctgcc aaaaaatcta
    40621 acgaatctaa taaaaagtct tcaatagcta atgtactatt gatcgtttcg atcaaatcca
    40681 cttgccgatc tttcaaaaat aggtggccat gcttatccat aaaaccgtaa tctccgctat
    40741 cccaccaatc gccataaaca ttttcctgga accgtgcatc ttccttgtaa taagtcaaag
    40801 cccgtccttt agacagtaat tgtatatgac catctgtgtt ttcaggtagg acattcccaa
    40861 ctgaatctgt aatccgagcg cttgtcatgt cttccagtcc aacgcccata tctcttgcat
    40921 cacttgtttt caaactctct agtgtatgag ccttaagaat cattggtccg cattcacttt
    40981 ggccatatac ttgaagaaat accgcatcct gtgccatcga cgttcgcaaa aatgtcttca
    41041 ttgtctggtt gtttatagca tcaaaagtgg agtgatagta tcggataccc gaaaaagctt
    41101 ctggatgctc cttagcagta aaacgccact gaacaaagtt attcggatgg gtctctagtg
    41161 caattggccg gtgagcttcc aacatatgaa caacttttga actttgtgcg tttgctaaag
    41221 gcatcatagg aaatcccatg gacatcaaag aagagatacc aatattgaat ctggaatgca
    41281 ctggagagat gtgaaaagcg attaatttct tttccgaaat ttttgtgaaa actgtttttt
    41341 gccatttcgt ccgccatccc atagaatgag cagaatgaca aataagttta gggataccag
    41401 ttgtaccaga agtatgggtc atataagaaa tttcattttt gtctaaaaat gattgagaaa
    41461 cagacaaagc aggttgttcc attaaataaa gaataggaat acccgtggtg ctagttaatt
    41521 ttaatatcct aaagaatatc tcaaagttcc ttcaaaacgt cgttgtgctc gacttaatag
    41581 aacactgtaa gccagtaata tgctttcaaa tctactctct agccctttaa cagaacgtga
    41641 gttgaaattt tgacaaccta atttttctaa ggaag
//
LOCUS       DARBEC010000015        41166 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.015, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000015 DARBEC010000000
VERSION     DARBEC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 41166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 41166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..41166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.015"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(451..1233)
                     /locus_tag="SCL80_000759"
     CDS             complement(451..1233)
                     /locus_tag="SCL80_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106647.1"
                     /translation="MENSSNSAEVNVSTSTSEVVKCSQETTSLPEELLGDWQINNKDA
                     GIEISDSKAVFRIGRSQVESKDYYVDVNNKMLLHVYFSFEYDSVKYIEVSIERTGYSE
                     FISKSVGILTMHTKDGQYISLGVKRVSGENNETEAVTEQTMDERIDSSKSNETEVDTG
                     KTIDERIDSFIETYTAIPPTWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNA
                     VTEQVGKDIPLRLLENGKKDYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(1298..2134)
                     /locus_tag="SCL80_000760"
     CDS             complement(1298..2134)
                     /locus_tag="SCL80_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1106648.1"
                     /translation="MRVIKTLNNSLILCLDKQGKEVILMGKGIGFQLKKGDLVPADKI
                     QKVFKLDSLSDALKKDYIKLFEAAPDDLIICVKRIIDYANAKFDFKLNKSLFFTLVDH
                     LKHAIERSQKGIVFQNRLLIEIQKYYPKEFEIGCYAIQLINKKMNVQLPEEEAGNIAF
                     HIVNAQSEQANMEETMLSVRALKDILAMLGYLFPEKKVDKDSMLYLRFVTHLQFFIAR
                     MIKKEELPEKDDFLLASVKQRFPKEYRAALKIKEYVQEEMGASANQDELLYLTLHISR
                     VY"
     gene            complement(2152..3561)
                     /locus_tag="SCL80_000761"
     CDS             complement(2152..3561)
                     /locus_tag="SCL80_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010757186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1106649.1"
                     /translation="MQYKQLSAFPEKFLWGASISAYQAEGAYQEDGKQPSIIDKYQHT
                     EGVANFEVASDFYHHYKEDIQLFKELGLKAFRFSIAWTRIMDIENNKTNQKGISFYHQ
                     VIDECLNQGIEPIVTMYHFDLPYFLEEQGGWLNRATIDYFVDYVQVLLTEYGDKVNYW
                     LTINEQNTMILHPGAIGLPPSGELPSKKELFQINHHVLLAQARVIHLYHQLNLKGKIG
                     PAINLTAMYQATSSPEDAIAAHNWETLRGWSFLDVAVRGKYNYLFENYLNDRGLYPKI
                     EAEDQAILSSGKPDFIAINYYSTATIAASKNDGSDVSARAGDQQIMLGEEGVYRAAEN
                     PYVDKTPYGWVVDPTGLRLTLRKLYDRYDLPILITENGYGAPDIVEEDRRINDQDRID
                     YLAKHILAIQAALTDGVDVFGYLPWSAIDVVSTHQGFNKRYGFIYVDRTDENLKELKR
                     IKKESFFWYQKVIARNGLA"
     gene            complement(3575..5398)
                     /locus_tag="SCL80_000762"
     CDS             complement(3575..5398)
                     /locus_tag="SCL80_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1106650.1"
                     /translation="MDYREVGRNVLQQVGGKENVVSLTHCATRLRFELKDKRKADTKA
                     LEKTLGVISVVDSGGQYQVVIGNEVQTAFKEIQKEVGTSIKKGPSSDNQESIISRLIS
                     VISTTFTPMIPAITGAGMVKAILAVLTLTGVLSDSSQTYIILNTIADAAFYFMPVLLA
                     YGAAIKFDVNPILAITVAGILLHPNISQLFSTGDAVSFLGIPVLAADYAGSVLPIIFT
                     VWIMSYVEPFADRISPSFIKFFTKPMLVFLIMGPLALVVIGPFGTLLNDLIAMGAEWI
                     NGKAAWLIPFLMGGLQPFLVVTGTAWAMTPIATMQLSSTGSEMINGPGMLASNIAQSG
                     ATFAVAAKTKNKELKQLAVSTGITALMGITEPSLYGVTLKLKKPLVASMIGGAIAGLY
                     AGLSGLVRYAFVSPGLAALPAFIGENPMNIVHAIITCVIAFVATFIIAYLIGFDDTSD
                     ESEQTAIAEAVIVENPVKGTVCPISEVNDAVFSKGLLGKGVAIHPEENSIYSPVEGEV
                     ITILESKHAIGLRSKEGIELLIHVGIDTVNLKGEPFEVFVKAGDQVSAGQLLINADFA
                     AIRNAGYDDTVIVVVTNSENYAGISEASGSLKEHEPLMQIS"
     gene            complement(6241..6567)
                     /locus_tag="SCL80_000763"
     CDS             complement(6241..6567)
                     /locus_tag="SCL80_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106651.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSKMTGTSGLEKSSKPLVTQ
                     ILQEQYNLNRSCDDVTITHENGDNTYRAKAILDNGSAININIEYYPKKDRIYVEIPYA
                     EVLMLN"
     gene            complement(6613..6975)
                     /locus_tag="SCL80_000764"
     CDS             complement(6613..6975)
                     /locus_tag="SCL80_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010717431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEG1106652.1"
                     /translation="MYCRKCGKEITDESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEKMGTILM
                     IAGVAGAIFGVLLGMSMY"
     gene            complement(7012..7227)
                     /locus_tag="SCL80_000765"
     CDS             complement(7012..7227)
                     /locus_tag="SCL80_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1106653.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPMNELFSDVFNKVNS"
     gene            7354..7689
                     /locus_tag="SCL80_000766"
     CDS             7354..7689
                     /locus_tag="SCL80_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1106654.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAEKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVVVDYEEEFLALYRSLSTEDAVRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(7765..9762)
                     /locus_tag="SCL80_000767"
     CDS             complement(7765..9762)
                     /locus_tag="SCL80_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106655.1"
                     /translation="METSMINIPIYVDQEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSVEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFNHKRSNVYRLDVFNATSVIQKLNEDILPLLEKIHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVGDNSVKNALIKNRGYFVKQGFTLERASKDIKKLYNVKASSSFLIYLAKGAGI
                     DETIKELILSGAGIVPLYQFTSVTRDDGRRVRKRKDPIVLN"
     gene            complement(9860..10135)
                     /locus_tag="SCL80_000768"
     CDS             complement(9860..10135)
                     /locus_tag="SCL80_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106656.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            10367..11101
                     /locus_tag="SCL80_000769"
     CDS             10367..11101
                     /locus_tag="SCL80_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1106657.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            11140..12282
                     /locus_tag="SCL80_000770"
     CDS             11140..12282
                     /locus_tag="SCL80_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1106658.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SCL80_000771"
     CDS             complement(12377..13768)
                     /gene="sufB"
                     /locus_tag="SCL80_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEG1106659.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKGEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(13773..14243)
                     /locus_tag="SCL80_000772"
     CDS             complement(13773..14243)
                     /locus_tag="SCL80_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEG1106660.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(14230..15465)
                     /locus_tag="SCL80_000773"
     CDS             complement(14230..15465)
                     /locus_tag="SCL80_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1106661.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SCL80_000774"
     CDS             complement(15462..16748)
                     /gene="sufD"
                     /locus_tag="SCL80_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEG1106662.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SCL80_000775"
     CDS             complement(16761..17534)
                     /gene="sufC"
                     /locus_tag="SCL80_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEG1106663.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(17731..18579)
                     /locus_tag="SCL80_000776"
     CDS             complement(17731..18579)
                     /locus_tag="SCL80_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEG1106664.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(18603..19289)
                     /locus_tag="SCL80_000777"
     CDS             complement(18603..19289)
                     /locus_tag="SCL80_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106665.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(19282..20316)
                     /locus_tag="SCL80_000778"
     CDS             complement(19282..20316)
                     /locus_tag="SCL80_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1106666.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(20379..20635)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(20711..21049)
                     /locus_tag="SCL80_000779"
     CDS             complement(20711..21049)
                     /locus_tag="SCL80_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEG1106667.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(21051..21407)
                     /locus_tag="SCL80_000780"
     CDS             complement(21051..21407)
                     /locus_tag="SCL80_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEG1106668.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(21413..22600)
                     /locus_tag="SCL80_000781"
     CDS             complement(21413..22600)
                     /locus_tag="SCL80_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1106669.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(22791..23636)
                     /locus_tag="SCL80_000782"
     CDS             complement(22791..23636)
                     /locus_tag="SCL80_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1106670.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            23870..24835
                     /locus_tag="SCL80_000783"
     CDS             23870..24835
                     /locus_tag="SCL80_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEG1106671.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            25212..26255
                     /locus_tag="SCL80_000784"
     CDS             25212..26255
                     /locus_tag="SCL80_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEG1106672.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(26317..26781)
                     /locus_tag="SCL80_000785"
     CDS             complement(26317..26781)
                     /locus_tag="SCL80_000785"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEG1106673.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            27027..27974
                     /locus_tag="SCL80_000786"
     CDS             27027..27974
                     /locus_tag="SCL80_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1106674.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(28185..30257)
                     /locus_tag="SCL80_000787"
     CDS             complement(28185..30257)
                     /locus_tag="SCL80_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1106675.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(30190..30567)
                     /locus_tag="SCL80_000788"
     CDS             complement(30190..30567)
                     /locus_tag="SCL80_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEG1106676.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(30696..31274)
                     /locus_tag="SCL80_000789"
     CDS             complement(30696..31274)
                     /locus_tag="SCL80_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1106677.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            31406..32698
                     /locus_tag="SCL80_000790"
     CDS             31406..32698
                     /locus_tag="SCL80_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1106678.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(32758..33876)
                     /locus_tag="SCL80_000791"
     CDS             complement(32758..33876)
                     /locus_tag="SCL80_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEG1106679.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="SCL80_000792"
     CDS             complement(33890..35311)
                     /gene="arcD"
                     /locus_tag="SCL80_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEG1106680.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLVTDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="SCL80_000793"
     CDS             complement(35557..36678)
                     /gene="mnmA"
                     /locus_tag="SCL80_000793"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEG1106681.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            37082..37681
                     /locus_tag="SCL80_000794"
     CDS             37082..37681
                     /locus_tag="SCL80_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEG1106682.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="SCL80_000795"
     CDS             complement(37819..39105)
                     /gene="efmA"
                     /locus_tag="SCL80_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEG1106683.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLTGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(39376..39720)
                     /locus_tag="SCL80_000796"
     CDS             complement(39376..39720)
                     /locus_tag="SCL80_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEG1106684.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(39857..41002)
                     /locus_tag="SCL80_000797"
     CDS             complement(39857..41002)
                     /locus_tag="SCL80_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1106685.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
BASE COUNT        11848 a         8652 c         7193 g        13473 t
ORIGIN      
        1 cccttttata aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg
       61 aaaggagtct aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt
      121 ctacatagtt atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt
      181 cataaaaaaa cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca
      241 ctaaaatcac tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc
      301 tccgtttcat ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc
      361 ccagtagctt atcctaagct actggagatg tataagtacg gtcatataac aacggggtag
      421 ggtaaataaa tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt
      481 gtaatcagtt acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag
      541 taaccgaagt ggaatgtctt taccaacttg ctctgtcact gcattactga tacctgttag
      601 attagctaat aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat
      661 cgtgattcct ttatcttcat ttacaagctt ccaagtaggt ggtatagctg tgtatgtttc
      721 gatgaatgaa tcaattcttt catctatcgt cttcccagta tctacttctg tctcattact
      781 cttagatgaa tcgattcttt catccatcgt ctgctccgta actgcttctg tctcattatt
      841 ctcaccggaa actctcttca cacccaaact tatatattgt ccatctttag tgtgcatagt
      901 taaaattccc acgcttttac taataaattc actataacca gttctttcaa tagagacttc
      961 aatgtatttc acactatcat actcaaaaga aaaatagaca tgtaaaagca ttttgttgtt
     1021 tacatctaca taataatcct ttgactctac ttgagacctt cctatacgga atacagcttt
     1081 actgtctgat atctctatac cagcatcctt attattaatc tgccaatctc caagtagctc
     1141 ttctggcaag ctagtagttt cttgtgaaca ctttaccact tcactggtag aggtcgaaac
     1201 attcacctcc gcactgttag aagaattttc caaggaatca ggagccttag cacaaccaac
     1261 aagtctttat atattgtgca gccgatattt tcttcattca gtacaccctt gaaatatgca
     1321 gtgttagata aagcagttcg tcctgatttg ctgaggcacc catctcttct tgaacgtact
     1381 ccttgatttt caaggccgcc cgatactctt tcggaaaacg ctgcttcact gaagccagca
     1441 aaaaatcatc cttctccggc aactcttcct tcttaatcat tcgggcaata aaaaactgta
     1501 aatgcgtcac aaatcttaag taaagcatcg aatctttatc aacctttttt tcgggaaaca
     1561 gatatcctag catagccaag atatccttta gtgctcttac cgagagcatg gtttcttcca
     1621 tatttgcttg ttcactttgg gcatttacaa tgtgaaacgc aatgtttcca gcctcctctt
     1681 ctggcaactg aacattcatt tttttgttga tcaattggat agcatagcag ccgatctcaa
     1741 attcttttgg gtaatacttt tgaatttcaa tcagcaggcg attttgaaaa acaatgcctt
     1801 tttgactgcg ttcaatggca tgcttcaagt gatcaaccaa ggtaaaaaat agacttttat
     1861 tcagcttaaa gtcaaacttg gcgttggcat aatcgataat tcgcttgacg caaataatta
     1921 gatcatccgg agcagcctca aacagtttaa tgtaatcctt ttttagagca tcagaaagtg
     1981 aatctaattt aaatactttt tggattttgt cagccggaac caaatcgcct ttctttagtt
     2041 gaaatccaat ccctttcccc atcaaaatta cttctttgcc ttgcttatct aagcaaagaa
     2101 ttaaactgtt gtttaacgtt ttaattactc tcattagatt caccgttctc tctaagctaa
     2161 gccatttctt gcgattactt tttgatacca aaagaagctt tcttttttta ttcgttttaa
     2221 ttctttaaga ttctcatcgg ttcggtcaac atatataaaa ccataacgct tattgaagcc
     2281 ttgatgggta ctaacaacat caatcgctga ccaaggtaaa taaccaaaaa catctacacc
     2341 atctgttaaa gctgcttgaa tagccagaat atgtttggct agataatcaa ttcgatcctg
     2401 gtcattaatt cgtctatctt cctcaactat gtcgggtgcc ccatagccat tttcagtaat
     2461 taaaataggt aaatcatagc gatcgtacaa tttcctcaat gttaatcgta gtccagtagg
     2521 gtcaactacc cagccatatg gcgttttatc tacataagga ttttcagctg cccggtagac
     2581 accttcttct cccaacatga tctgttgatc ccccgctcta gcggaaacat ctgatccgtc
     2641 atttttgctg gcagcgatgg tggcagttga ataataatta atcgcaataa aatcgggttt
     2701 accggaggat aaaattgcct gatcctctgc ctcaatttta ggatataatc cacgatcatt
     2761 caaatagttt tcaaacaaat aattgtattt tccccgaact gctacatcta agaaagacca
     2821 gcctctcaat gtttcccaat tatgggctgc aattgcatct tctggactgg aggtagcttg
     2881 atacatagcc gttaaattga ttgctggacc gatttttcct ttaagattca actgatgata
     2941 caagtgaatt actctagctt gagctaataa aacatggtga ttaatttgaa agagttcttt
     3001 ttttgaaggc agctctccac ttggtggcaa accaatcgct ccaggatgaa ggatcatcgt
     3061 gttttgctca ttaattgtca gccaatagtt caccttgtca ccatactcag taagcaatac
     3121 ctgtacatag tccacaaaat aatcgatagt tgctcgattt aaccaaccgc cctgctcctc
     3181 tagaaaataa ggtaaatcga aatggtacat ggtgacaatc ggttcgattc cctgattcag
     3241 gcattcatca attacttgat gataaaaaga aattcccttt tgattggttt tattattttc
     3301 aatatccata attcgcgtcc aagcgatgga aaagcgaaaa gcttttaagc ctaattcttt
     3361 aaacagctgt atatcttctt tatagtgatg gtaaaaatcc gaggcaactt caaaattagc
     3421 taccccttct gtatgctgat acttgtcaat aatagacggt tgtttcccat cctcctgata
     3481 ggcaccctct gcctggtagg cactaatgga ggcaccccat aaaaacttct ctggaaatgc
     3541 tgaaagctgt ttgtattgca caaaaatcct ctccttaact aatttgcatc aatggttcgt
     3601 gttcctttaa gcttccgctt gcttcactaa ttcctgcgta attttcactg ttggtcacta
     3661 ccacaatcac tgtatcatcg tagccagcgt ttctaatggc agcgaaatcg gcgttgatta
     3721 ataattgtcc tgctgacacc tgatctcctg cttttacaaa tacttcaaag ggttcccctt
     3781 ttaaattcac ggtgtcgatc cccacatgga tcaataattc aataccttct ttagaacgta
     3841 gaccaatcgc atgtttagat tctaaaatag taatgacttc tccctcaaca ggtgaataaa
     3901 tgctgttttc ttctggatga atggctacgc cctttcccaa tagtccctta gaaaagacag
     3961 catcgttcac ttcagaaata ggacacacgg ttccttttac cggattttca acaatgactg
     4021 cttcggctat tgccgtttgc tctgattcat cagaagtatc gtcaaatcca attagataag
     4081 caatgataaa ggttgcaaca aaggcgatta cacacgtaat aattgcgtga acaatattca
     4141 ttggattttc tccaataaag gctggtaagg ctgctaagcc aggggaaaca aaggcatatc
     4201 tgactaaacc gctcaagcca gcatacaaac cagcaattgc accaccaatc atggaagcaa
     4261 ctaatggttt tttcagcttc aaggttactc catagagtga aggctcagta attcccatta
     4321 aagcagtgat cccagtggac acagctagct gcttcaactc tttgttttta gttttcgcgg
     4381 ctacagcaaa ggtagcacct gattgagcaa tattagaagc cagcattcct ggcccattga
     4441 tcatttcaga acccgtactg ctcaattgca tagtagcgat tggcgtcatt gcccaagctg
     4501 tacccgtaac caccaaaaat ggctgcaaac cacccatcaa aaatggaatt aaccaagctg
     4561 ctttgccgtt aatccattca gcccccattg ctataagatc gttcagcaac gttccaaacg
     4621 gtccaatgac aaccaacgct aaaggcccca taattaaaaa taccagcatc ggcttagtaa
     4681 agaatttaat aaacgaaggc gaaattcgat ccgcaaaggg ctctacatag gacatgatcc
     4741 aaacagtaaa gataatcggt aaaactgatc ccgcataatc tgcagctaat accggaattc
     4801 ctagaaaaga aacggcatcg ccagtagaaa aaagctgtga aatatttgga tgaagcaaaa
     4861 tgccagcaac agtaatcgcc aaaattgggt tgacatcaaa ctttatggct gccccatagg
     4921 ctaatagtac tggcataaaa taaaaggctg catcagcaat agtattcaaa atgatgtaag
     4981 tttgactact gtctgatagt acaccagtca aagtcaagac ggccaaaatg gcttttacca
     5041 tcccggcacc tgttatagct ggaatcatcg gtgtaaaggt ggtagaaatg acactaatca
     5101 atcttgaaat aatactttct tgattgtctg aagaggggcc ctttttaatt gaagttccca
     5161 cttccttttg aatttccttg aaggctgttt ggacttcatt gccaatcacc acttggtatt
     5221 gtccaccgct atccaccaca ctaattacac caagtgtttt ttctaaggct ttcgtatcgg
     5281 ctttcctttt atctttgagt tcaaaacgca gtctggtggc acaatgggtt aagctgacca
     5341 cattttcttt tcccccaacc tgttgcaata catttcttcc tacttctctg taatccatta
     5401 ttctctctcc ttaaagcagt aaaaaagacc aagtcaaacc acgccccaat caacattgaa
     5461 gtgttagttt gtcttggtct tgcctgcata atcagtaaca tgccaataac gttattttgc
     5521 tgtaatccaa taatagcaca ttatgatagc gcaatcaaca gggaatttaa ttttttatct
     5581 tttcgtttga ccgtataggg taatataaca gttagatcac atcaactagg gtatatctgc
     5641 gtatacttac tgctaaaata agtatatcta atattaacta taccagtaaa accattccta
     5701 cataaccagc gggaaattcc caaaatcgtg aaagcatcaa aaatgcatat aacggatcac
     5761 acaacttcaa caattctgct cttgggaaaa attgatgaat ttggtgagta aaggatggaa
     5821 taaacagcat tccaacaata taggcaacaa tagtgatact tgtttttttg gtaaaaatca
     5881 gtaagcaata gccaatttca atcattccga gatataaagg tatcgataga atgatttggg
     5941 ctagtattct taaaaattaa ctagcaaatg tttaaactga tttatttcta atctacatgt
     6001 agtaagtatt tcaattccac caattatcaa taaaaatacc aaacatatcc ataataattt
     6061 tttctttttc atttctttta tattgttata acagtgaata acataaaaat tcccgacaat
     6121 cattgtaata tttttttgtc acctgtatta gtatttaatt tataggaaac tataaaaact
     6181 ccctagtgaa tttacacctt tgggagttcc tgtttggacg gtatgcataa tacatttaaa
     6241 tcaatttaac attaacacct cagcatatgg tatctccaca tatatacggt ctttctttgg
     6301 atagtactca atatttatgt tgatagcact tccattgtct aatatagctt tagcccggta
     6361 tgtgttatct ccattttcat gagtaatcgt cacatcatca catgatcgat ttaagttgta
     6421 ctgttcttgt aaaatctgtg ttaccaatgg ttttgatgac ttctctaatc cagatgtacc
     6481 tgtcattttt gaaaaaataa tacctataat agccaagaca acaataccta taatgacaaa
     6541 cttcttgttc atatttactt tattcaactt tttatctcct atctccaaaa taatttgctc
     6601 ttatccctct tatcagtaca ttgacatccc taaaagtaca ccaaagattg ctccagctac
     6661 ccctgctatc atcaaaatcg tacccatttt ctcatctttt tctcgatata agtagcccat
     6721 aataacagca acagcaccaa atacaacagg aataaatgct aacgatacta ccatagaaat
     6781 ccaaccaata atcaaataca gcttccctgt ctcttgtcca tgtacgattt gttccttcat
     6841 ttcattaatt gtctgctcag tacttttctt tactgtgtct gttccggttg tctcatctgt
     6901 ttcttgtagt gctccacaaa actggcaata cttactttca tctgttattt ctttgccaca
     6961 ttttctacaa tacatgtctt ctcctcctaa aatcattcaa ttgcttatta gttaactgtt
     7021 tactttatta aatacatcag aaaacagctc attcattggt acacttaata tctcagaaag
     7081 catcttcatt tgatagggtt taggaatcgt tctccctgtt tgccacgaac ttacacattg
     7141 ctgtgtaaca ttcatttttt ttgctaactc cgtttgagaa agtccttttt cttttctgta
     7201 tcggctgaga ttacttactt ttttcatgat tctcccctcc tgttttatta tatacaacaa
     7261 tatgatgtat ttcaactcat aaaatataaa aaacttcaaa taccattaca aatacaacaa
     7321 cttgttgtac tatactgaga tgggggtgta ttaatgaaac agaaacaata tgcaaaggtc
     7381 attagagaaa agcgaaaagc tctcgggtgg acacaaaaag aattagctga aaaaatcttc
     7441 tctacacaac aggcagtagc aagatgggaa aattctgtaa cagagcctaa cctcgatagc
     7501 ttaacagctc tttctagagc cctaggaacc cctgtaagcc attttctaga caatgttgtc
     7561 gtagattatg aggaagagtt tttggctctc tatcgttctc ttagcactga agatgcagtt
     7621 cgaacaattg actatatgaa gctattaaaa aggcaagaaa atgaacgcaa tcagctattg
     7681 aaagattgaa aaataaataa cccaccgaac tttaacggtg ggtgtattga gatgaaataa
     7741 gattaagagc tatggataac ctgtctagtt taatacaatt gggtcttttc tcttccttac
     7801 tctccgtcca tcatctcttg ttacacttgt aaattgatac agaggaacta tcccagctcc
     7861 tgataatatc aattctttta ttgtttcatc tatccctgcc cctttcgcta aatatattaa
     7921 aaaactacta cttgctttca cattgtatag tttcttaata tccttagaag ctcgttcaag
     7981 agtgaatcct tgtttaacaa agtagcctct gttttttatt aaagcatttt tcacgctgtt
     8041 atccccaaca ttacgtaggc gttcacaaac agtgttaata agttctttct ctgtcattag
     8101 atatgtccct tgccacgata agccctgtaa aacttcatgt gcttcatcta aaatcaattg
     8161 tttttccttg ctgagttctg atcgggaatt ttgaggaaat actcttttag cctctttttc
     8221 gttaaaacat atcttaaccc attcataaga aaaatattga tatgtaaaat gaattttttc
     8281 cagaagtgga aggatatctt cattcaattt ttgaatcaca gaagttgcat taaaaacgtc
     8341 taatcgatag acattgcttc tcttatgatt aaagtttcct aacgctttct cttcctttaa
     8401 cctatttagg tattcgagca tcttcaaagg aacatcttca tcatctaatt tttttatcaa
     8461 tcctataaaa gtcataagtg atagtaactt attacattta tctatgtcat aagttttacc
     8521 atagtaagaa ctcatttttt tagctaattg ttcccccgac aaaatacaag tcacactttc
     8581 cttactatca tcaaaaaaat taacatcaga aagcaagaca tcatagacac gtcgcatttc
     8641 ctctagataa ttccttattg tcctgaaaag tttgtctttc attgattcaa gagtgtcaaa
     8701 aagtactttt gattcctctt tcaattttcg aaaatgtgta ggatacaagt ctaggttctc
     8761 taatagccaa ttaaatgttt ctacccgact gtctttcttt aaaattagca tcacaactcc
     8821 aataaaattt tcactgatat cacaattact ggaatgacaa taaaaaatgg aatgcccagt
     8881 tttactggta aaaatactag cagagggatt atgatcgttg tgaaacagac atcttaagtt
     8941 tttacttgac ttaagtaatt tattcatatc aatggttaac aatctttcat aaatctcatt
     9001 aaaagacatg tcggaggaat caaacaggca gtctttaaac cactgtttca tttcttcttt
     9061 gtcgtcattt tttatcaact taacaaaatc ggtttcattg actagttcat actctctccc
     9121 tgaatcactt tgacaatttt tagaacatgt catggtaaaa tcaatcggaa gctcagtaat
     9181 atctaacatg ttgtcttcat taattattat cactgcatct gacttatatc ctccaaaaaa
     9241 cattctattc gaagcattta cattgtcatc acacccgggg atgttctttt tcaagtattc
     9301 gtaaacagca accattttac catagtcagt aattgcttct tttaatagaa agacaatctt
     9361 gtatttttct agattttcag agtaccgaat acttttttgt atcgcaaacg cagaattatg
     9421 aactaaattg atagataaaa ccttttctac gcttaagtat ctactatcat ctttactcaa
     9481 atctactacc ttccccttct catctttaac agtgttatcg acatccagaa taattactct
     9541 ggtttccaca aaactctcct tgtcattgcc tttattcttt gtcttgcttg gaataaatga
     9601 ttgtgatgaa tttagtatct ctacaaactc atctcttgta acttctctat agttatctaa
     9661 acacctatct cttacatcag ttttttcttt ttttgtaact ggcttctcta ataatttttc
     9721 ttgatctaca taaattggaa tattaatcat tgatgtttcc atttgtgttt cctccttata
     9781 aaattctgtt aaagactaaa acatacagat aataaaaaca aactctttat tactatatgt
     9841 tttagtctta tttgtttact caacctttat accgttccat gaaactatca atatcagatt
     9901 ttttaaatct aatgaccgta tcaattttta taacaggtaa cccgtattga gaaacatatt
     9961 ttctaatcgt tcctactgat acacctagat attcacttgc ctgctgatag tccatatact
    10021 ctagagtgac tccagaaata atgtcttcta gcataggctt tagcactgta attatctctt
    10081 tcacttgttg ttcaacttgt atagaaacaa tgctatcaat gatttgtaga gacatatttc
    10141 cccctccttt cgtgattgct tgaacaaatt ttctaagaat tatcttcgta ttttttgctc
    10201 aaaataaaag tattttgtta ttaaccgatt aatcaaacaa aaaatgatta ccagttcatc
    10261 gacatacaca taatacctcg ataaaaaagg ggtgtcaaca gctttgtaaa atttatttgc
    10321 aacacccctt ttttgaggta tactaatttt aggaggtgtg gtcaatatga taaaacgtcc
    10381 accaataaat tatttagaac gaaaaaagat actcggaaca aaaataaaag ctataagaaa
    10441 gagtaaaaaa ttaacacaac cagcatttgg tcttatgatt aacaacggtc aattaattga
    10501 caaaaaaact atctacgaat gggagaaggg aacctattta ccaataccag agcgattatc
    10561 ccgaattgct gatttgggaa atatgagtat agaggagtta gtttgtggca atgtagaaga
    10621 atatattttg ggaattattc tttatagaga tagcattgta ctagatggta taacatttcc
    10681 agataaaaac ctgttccagc atcttcgtca acagttccca ccagtacact ctaatttaga
    10741 tacatggctg gatagatatt ccaaattaga accagagatg caagaattta ttgctaacaa
    10801 gacttgtaat aaagttaaaa atgaaaaaat tagtcttttc aatatcttaa aaatagaaga
    10861 attattcatc aatgctattg tagaagaatt tgacaataac attcttttcc tcactagcag
    10921 tatagaagaa ctactagaaa gaatggtaga tgaatggcta cctattcaat tgaaagacat
    10981 gtcttatccc gaagaagctg tgagagaaat tactgataat ataaacaagc tagaacaaac
    11041 gatttcatca attgggaaaa aatacactaa gaaaaaaatg aagggaggtg ataccatcta
    11101 gtggtaagta ctccttgtaa actaagagga gatgatttaa tggcaaaaat atcaaagtac
    11161 aaaaagaaaa acggtcaagt ggcgtggatg tataaaggac acattgccac cgatccacga
    11221 actggtgaaa aaataaatac tacgagaaga ggttttagta gcaaacaaga agctcaaaaa
    11281 gattatgacg agtacagaca tcaaatatta tacggtgtta aaaagaaagc acctgatatg
    11341 acatttgaag acctgtataa tgaatggata actcatcagc gtaccagtgt taaagcttca
    11401 acaatcgcaa tctcagtaag atacgcaaat aatcaaatac ttccagcatt tggtaagctt
    11461 aaactatcga atatctctgt cccatattgt caaaaagtgg tagatgaatg gcacagtaaa
    11521 tatgaaagtt acgattatat gagaaaacag actgcacaaa ttttacgcta cggggttgca
    11581 atgcaatata ttgacaataa tcctatggaa aaaacacttc ttccacgaaa aaaagagtat
    11641 gagaaaaatc gtaaatttta ctcaaaagag gagcttaata atttactgga tgcttttaaa
    11701 gattttggta acatgaaaca atacgccttc ttcagattat tggcatacac aggtatgaga
    11761 aagagtgaag tactcgcttt acagtggaaa gacatagaca cgtttaataa agaactacat
    11821 gtcaataaaa ctcttgctgt agatgaattt ggtaaagtta taattcaatc tccgaaaaca
    11881 agagcttcta gacgagttat ctctttagat actgagactc tttctatcct caacaactgg
    11941 aaattacagc aaaaagaaga gtatttaaaa cttggataca atactagctc taaagagcaa
    12001 catgttttta caacagttaa aaacactcta tacattccaa acactgttaa tgattggtta
    12061 agatatattt tgaaaaaata taacttgcct cgtattacac ctcatgggtt tagacataca
    12121 catgctagtt tattattaga agcaggagaa tctgtcaagg tagttcaaca acgattaggg
    12181 catgaaaata gtaaagtgac attagacatc tatgcacata ttacaaataa tgcacctaag
    12241 aaaacaggtc aagactttgc tgatatgatg gctcatcagt aaaagagctg gtcaaaatct
    12301 ggtcatttct agtatacagg caaaacaaaa aagctagaac ccttatatat caagggttct
    12361 agctcctata acaggtttac ccaacactgc cttccatctc ataactgatc aatcgattga
    12421 gttcgactgc atattccatt ggaagttctt tggtgaatgg ttcaacaaag cccatgacga
    12481 tcatttcagt tgcttcagat tcggtcaggc ctcggctcat cagatagtag agttgttctt
    12541 ctgagatttt tgagactttg gcttcgtgtt ctaaagagac ttggctgttg tggatctcat
    12601 tgaatgggat cgtatcggat ttcgaccatt cgtccatgat gatcgtgtca cattcgatat
    12661 gagagatcga acccgcactg tttttggcaa aggttacttg tcctcgatag tttacttctc
    12721 cgccgtcttt tgaaattgat ttagagacga tagagctaga tgtatttggt gcattgtgga
    12781 tcatttttgc acccgtatct tggatttggt ttgctcctgc aaaggcgatg gagagcatgg
    12841 tgcctcgtgc accttttcca tctaagtaaa cacttgggta tttcatcgtt gtttttgcac
    12901 ctaggtttcc gtcgatccat tcaacggttg cgccttcgta tgcttttgct cgtttggtta
    12961 ctaagttgta cacattgtct gaccagtttt ggatcgttgt gtaacggcaa tacgcatctt
    13021 ttcttgtaaa gatttctacg atggcggcat gcaggctgtt acttgaataa gtaggtgccg
    13081 tacaaccttc gacatagtgg atgcttgctc cttcgtcaac aatgatcagc gtacgttcga
    13141 attgccccgt gttttccgca ttgatccgga agtacgtttg taatggaaca tcgactttga
    13201 cgccttttgg cacgtaaata aaggtcccac ctgaccatac ggctgaattc agcgctgcta
    13261 atttgttgtc ggttggcggt acaagttttg aaaagtactc tttgaataat tcagggtatt
    13321 ctttcaaagc agagtcagtg tcagtaaaga cgattcctaa cttttcgaat tcatctttca
    13381 tattatggta cacgacttct gattcatatt gtgcggaagc accggctaga taagctcgtt
    13441 cagcttctgg tataccaatc ttttcaaatg tatctttgat tttttctggt acgtcttccc
    13501 agtcacgagc gggtttgtca cttggttttt ggtaatattt gatggcatcg aaatcgatgt
    13561 ctgaaaggtc ggggccccat gtttgcattg gcattttgtt aaatgtttct agagatttca
    13621 gacggaattc aagcatccat tctggttcgc ctttgattct tgacatctca cggatcactt
    13681 cttctgtcaa gccttctcct gtactgaaaa ccggttcgac atcatcgtga aacccgaatt
    13741 tatattcttc taattctggt acgcccatgt tctcactcct cttccttgtg gtaatgatgg
    13801 aaagctgcgg taccatcgtt ttccattgct tttcctagtg ctttccaggc aagtgtcgca
    13861 catttgatcc gagcgggaaa tttagctaca ccaccaagca tagcagcatc gcctaattgg
    13921 tcgggatctt tgacttcttt tccttggaca agctgtagaa agtctacagt taaatgttct
    13981 gcttcttcta ttgattttcc gatgacggcg tctgtcatca tcgaggcact tgccgtactg
    14041 atggagcagc cactgccgtc aaaggcgata tttttgatga ttccgtcttt tacatcgact
    14101 tgcaattcga tcacatctcc acatgtgggg ttgttcaatt cgatcttgct tgtcgagtca
    14161 gtcaagcttc cacgatgatg cggatgggaa gaatgatcca agatcacttg acggtataaa
    14221 ttgtctaatt tagatagtcc catgttggaa aaactccttt gttgccatga ttgcttcaac
    14281 taaacggtcg gcgtcttgcg ttgtgttgta gaaataaaag ctcgcgcgag cagtcgctga
    14341 tacttcaagg tacttcaata gcggttgcgc acaatgatga cctgctctga cggctacccc
    14401 ttccatgtct aaagctgtcg cgacatcatg gggatgtaac ccatccaaat tgaatgcaac
    14461 gactcctgtc cgaaaattcg ggtcttgtgg accataaaca gtcaatccat caattgcttg
    14521 taattttggc aacacataag cggctagttc ttcttcatat cgatggatat tgtccatacc
    14581 gagtttttgc aaataatcga ttgcatggcc taatgcgata gcgccggcga tatttggtgt
    14641 ccctgcttcg aatttccaag gtagttcttt ccatgtactg tcgtataatt cgacaaaatc
    14701 gatcatttcg ccaccaaatt caacaggttc catttcttct aaccattttc gtttaccata
    14761 taaaacacca atacctgttg gtccgcacat tttgtgtccg ctgaaagcaa agaaatcaca
    14821 gtctagtgct tggacatcaa tggccatgtg cggggcagat tgagcaccat ctactacgat
    14881 gaccccgccg ttttcatgga caagttcagc taattcttga acaggattga agacacctaa
    14941 gacattggat acatgagcaa tggagacgat tttggttttg tccgtaatct ttttcgctgc
    15001 atcctccata tccaggaagc cttcttctgt tagttcgata tatttcaaga cagcacctgt
    15061 tcgttttgcc aactgctgcc acgggataac attagaatga tgttccatgt aagagagcac
    15121 tatctcgtct cctgctttga tatagcggtc gccgaaggtt ctggctaccc aattcaagct
    15181 tgtcgtcgta ccacgagtaa acagcacttc tgctgtttct tttgcatgga tgaacgcccg
    15241 tactttttct cttgcttgtt cataatcatg ggtcgcgcgt tctgctaacg tatggacacc
    15301 gcgatgaaca ttggcgttat cgtgttcgta atagtacgct aatgcctgga gtacttgttt
    15361 tggcttttgg gtcgtcgctg cattatccag ataaactaaa ggttcatcat tgacgtcttg
    15421 aaataagata ggaaaatcat tgcgtagctt tttagcatcg atcatccatt caacttccct
    15481 tcgattactt cgactaattc tttccgaact tgttctacag ggattgctgt taagacagaa
    15541 ccgaggaatc ctcgaatcac taagcgctct gcttcttctt tatgcaatcc tctactcatc
    15601 agatagtaca tttcttctgg atcaactcgt cctacactgg cagcatgtcc agcagttact
    15661 tcgttttcat cgatcaaaag aataggattc gcatcaccac gtgctttatc agaaagcatc
    15721 aagacacgac tttcttgttg ggcatctgca ccttttgccc ctttcaagat gtggccgata
    15781 ccattaaagg tcaatgttcc tttttctcgg atcactccgt gttgcaaaat atgcccgatc
    15841 gtatgagggg ctttattggt tactcgtgta tcgattcctt ggatttgtct gcccgagctg
    15901 attgccacga ttttgacttc tgcgtgtgca ccttctcctg ctaaatctga gtcaaagtca
    15961 gcgatcacat gaccgtcatt catgacacca attgcccagt cgacagaagc atctcgtaaa
    16021 atgtgcccac ggcgattcat ataagaagta atattttctc ctaattgatc tacggctgaa
    16081 tatttgattt ttgaaccagc tttagcgatg acttccacga tgatattacc tgatgatttt
    16141 gctacttgtt ctttggttgt ctggaaacgt tccagataag aaaattcact atgttcatct
    16201 gccacgatca acacatgctt gaagaagtgc tcatcggatg cgccatcttg aatgaataag
    16261 gactcgagtg gttcatcgat cacgacattt ttaggcacat acaggaaaat accgctgttc
    16321 ataaaagcta catggagtgc agtcaattgg tcttcgttca tgtttactgc ttttttcata
    16381 taatattctt ggactagttc tggatattcg atcattgcag tgaacaagtc ggtaaaaatg
    16441 actccttttt ctgctaattc cacaggcagt tgttcaaaaa tcgtccatga accttgttgg
    16501 acaagcacag gattgtcttt cattgcatca aatgccggaa tcgtgcctgt ttcactgatg
    16561 gtttgtgttt ctttcacatc aaataacgac cagcgatgga atttcacccg atcgatatgc
    16621 ggcaatggca gttcatctgc tttcgctagt gcgtcttgac ggaaggttct catccaatca
    16681 ggttcgcctt tacttaaaga aaaatcattt accgcgtcaa gataatcgag ccagtttttt
    16741 tctttcattc ttgctccccc ttacgcttct tcttctttat agtcgattcc taattcttta
    16801 ctgataccag cgtagccttc tgcttccaga cgtttagcta ggtcagcatt ccctgtcatg
    16861 actactcggc cttccatcat gatgtgaacg acatcaggtg tgatatagtt caataatcgt
    16921 tgatagtggg tgatgatcaa tgcaccgaaa ttgtctccgc gcatttcatt tactccttta
    16981 gcaacgactt tcaaagcatc gatatccaga cctgaatcga tttcgtctaa aattgcaaat
    17041 gtaggttcta acatcaacaa ttgcaggatc tcattccgtt tcttttctcc gccagaaaaa
    17101 ccttcgttca gataacgttc agccatttct tctggcatat ttagtaaagc cattttttca
    17161 tctaactttt tcaaaaatgc cataacagaa attttgtcgt tttcatcacg tttcgcattg
    17221 atcgctgcac gcatgaattc cgcattcgtg atacctggta tctcacttgg atattgcata
    17281 gctaaaaata agccaagtcg cgcacgttca tctacttcca agtctaaaac atttttcccg
    17341 tcaaacaaaa tttcgccttc tgttacttca tagtttggat tgcccatgat agcggcagat
    17401 aaggtagatt ttcctgtgcc gttaggtccc atgatggcat ggatttctcc ggttttcatt
    17461 gttaggttta cgcctttaag gatttctttt tcctcgatcg acacatgtag attttttatt
    17521 tctaagacag acatgcatta tacccccaaa ttctctttcg ttctcactca ttttagacta
    17581 attgagaatg aagcgcaatg accttcctta ctaaaatcaa atatctttta tttattatta
    17641 aacaattttg atttctaaat tagaataggt ataaaaaaga tgagaaataa gccattacag
    17701 acttatttct catctttttt gttccatacc ttattttgca tcagccttta ctggtttaac
    17761 agagccgccc catttgtctg tgatccattc ttggacttct ttgcggtgaa gcacttcaat
    17821 cagttttttg attttgtcac tgtcttcatc acctttacga gtcgcaataa tattcacgta
    17881 aggagagttg tccttttcaa gcaatacggc atcttctaat ggattcaacc ctgcgccgta
    17941 agcgaagttt gcgttgattg caactaggtc accttcatca ttttggtacg tagaagctaa
    18001 aagtgatggg tcgatcgtat ggttgaactt caagttctta gggttttctt caatatcatc
    18061 gaatgttgct gtttccagat ctactccatc tttgactttg actaagccag catcttgtag
    18121 aattgtgatg atgcgtcccc agtctgattc agaatttgaa gtaatgattg ttgcaccatc
    18181 ttttaattcg gaaacatctt tgactttttt agagtacaag cccattggtt cgatgtggac
    18241 agcaccggca ttgacaaagt catatccttt ttctttgatt tctttgttca aataaggaat
    18301 atgctggaag taattggcat caatgtcgcc ttcttccaaa gattggttcg gtaatacata
    18361 gtcatcgaat ttgacgattt caagatcgat tccttcatct ttcaataatg gtttcacatg
    18421 ttccaaaatc tctgcgtgcg gtgtcggtga agcgccgact ttcaatgtcg tgtcgtctgc
    18481 tttatttgct gaatctttgc tgtcgctgtt gttaccgcat gctgctaatc ctacagtcag
    18541 caaaagcgct gctgcaaatc caaaaatttt ctttttcata tcctacatcc ccctatatcc
    18601 tattatcttt tgtctgtttt cttagttaag aaatcaccga ttccttgaac gataaatacg
    18661 attagtaaaa taattaaagt cgcaacgaat gtcactgtta gattaccacg ctggtagcct
    18721 tcctgccaag caagacctcc aagtccacct gcgccgatcg cgccggccat cgcagtaaat
    18781 ccgatcatcg agatcgttgt aactgttaat ccagaaatca atgcgggaat actttcaggt
    18841 atcaagacct tccagatgat ttcccagtaa cttgctccca ttgcatctgc tgcttccagt
    18901 acaccagggc tgacttcacg aaaagcaatc tctactagac gggcataaaa tggtgctgcg
    18961 gataaaacaa gagctggaat ggctgcttgc gcacctagca tagtgcctac gattgcttta
    19021 gtaaatggaa tgatcaaaac catcaggatc ataaatggcg ttgaccggaa aatattactg
    19081 atgatcgata cgattccata aatgattcga gcatacggtt tgtttttgcg tccaatagag
    19141 taaaggatca atcccaaaac taatccgatg atcgtcacta agaccattga gattaatgtc
    19201 atatatagtg tatcaaggat agcctgattc atcgtttccg ggttgatttg tttaaaatct
    19261 agatatgttt ctgaaaagct tttatccacg atggatcacc tcaattccta ctccttgttc
    19321 tttgatgaac acttctgctt gatctaattc tttggtttca ccagtcatca agacagtcaa
    19381 tgaaccaaag gaaccttctt tcacttgttt gatctttcca tacacaacgt tgacatcgat
    19441 gttatagcgt cgaatcgctt gggaaatgac tggctcgttg gcatttcttt cgctaaaggt
    19501 caaagttgcc aaacggccgc ttggattttc tttgatgagt tcagctaata gttctgtcga
    19561 atcagcttct ggttcgatgt cttgttgtac aaagcgtttg gttacttctt ttttcggttg
    19621 tctgaagaca gtcaatacgt ctccttcttc tacgactttc cccaactcca tcacagcgac
    19681 tttgttgcag attttgcgga tcacattcat ttcatgtgtg atcaagacga tggtcaaatt
    19741 caatttctta ttgatcgcta acaataagtc taatacttct tctgttgttt gcgggtccaa
    19801 tgcgctagta gcctcatcac aaagcaggat ctcaggatca tttgccaatg ctcgtgcgat
    19861 accgacgcgt tgtttctgtc cgccggaaag ttgtgaagga tatgcttgtc cgcgtccttc
    19921 taacccgact aaacgcaaca gttcttctgc ccgttcttta cgtttgctct ttggtacgcc
    19981 agctaattcc agcggcagct ggatattttc aagtacagtt cttgaccata acaggttgaa
    20041 atgctggaag atcataccga ttttctttcg gaatgtacgt aattctttgt tttttaattt
    20101 tgtaatatct tgatcgttta cgatcacttc gccgctcgtg ggaagctcaa gtccattcag
    20161 gagacggact aatgtacttt ttcctgcgcc agaatagccg acgattccat aaatatcccc
    20221 tttttcaacg tttagtgaaa cgtcattgac ggcttggatg tttccgtttt tcccattgaa
    20281 tgtctttttg acattcgtta gttggattaa tgacatgcca ttcacatctt tctcttatga
    20341 tttttcaaac aaaaaagcct tcgccctctt gccttaagca aaaggacgaa agcttgaact
    20401 tccgtgttac caccttgatt taccgttact tcgcaataac agcctcttcc agtacttgga
    20461 cttagcttct ttccgtatac tgtcgcgctg taacgggcac tcccgtagta agtttgctta
    20521 cgctcgctta ctctgctcta agaccatctt ccatctcatt ttcgttaccc attttcagca
    20581 aacagggctc tcttcaaacg tccgtgacat gtactcttct tttcaaagct ttctcgtttc
    20641 atataggatt gcttatgatt atagcaactg gaagaatcag tgtcaacttt tttcaagtga
    20701 ttttctgatt ttataattcg tcaaattctt ttggatcaac atctttgaaa cttaagatcc
    20761 acgcattcat ttcatctgaa tcattcaaga tattgatatc ttcaccgatt ttatcattaa
    20821 cagaagaaac tgtacccgtt agcgggctgg caaattcatt gaccgctttt tccgcttcaa
    20881 cttcgatcag aggttcatct ttttttagcg attgtcctgc ttttggcaga ttggcaaacg
    20941 tgatgtctcc aagttcttct tgcgcttcat ttgtcaaacc aacacagtat tcttttccat
    21001 taaataagat ccaaaggttg tcctttttct tcaaatgctt gcctgccatt ttatatcagt
    21061 tctccttcat aaattttttc attgaatcca attgcaacaa gctggtcctc tcgtaagatc
    21121 agtggtcgct tgatcagcat accatctgtc gataagactt ggctcgcttt tgaagccgta
    21181 aactcgccta cttgatcttt cagtccaagt tcccgatatt tcatcccgct tgtgttgaac
    21241 aggcgacgga ttggtacttg actcgtttct agccaagtct caatcgttga ggccttcggt
    21301 acttctttga tcatatcaac ggtttgataa ctaacgccat gctggtctaa ccacgctttg
    21361 gcttttttgc atgttgaaca tttcgggtac cagtaaaagg tgtacaattt tcttacctcc
    21421 ttgaagatct tgcaggtgct tcattttggt atctcatcga taatatcaac ccgataccga
    21481 tcatgttccc taaaatcgag gatcctccct gactaatgaa aggcaatggg atccctgtta
    21541 aaggaagtaa tccgatattt gctccaatat tctcaaagac atggaacagc agcatcatga
    21601 tcaaaccgct tgcgatatag gcgtaaaatt cattattcgt atcgaaacac acccgaatca
    21661 tacgatagat taaaataaaa tacaagaaaa tgacgaatgc accaccgata aagccgaagt
    21721 tttcaccgat aacggtaaaa atcatatcgg actcacgaac aggcacatag atattgctta
    21781 cgttgaatcc tttaccaaac aagccaccgg taccaattgc caaaagtcct tgggccggct
    21841 gataactcat tcctgacgta tcatggaaag ggtccagcca tgaatcaata cgtgcaaact
    21901 gataagattt gaatccaact ttataaagca agtctcttcc gccttcagtc gtaaccaaga
    21961 agatcgttcc tgctccaacg actactgcca agattactac aggtgcgatg atgcgccaag
    22021 agattccaga catcaggaaa atcccgccaa agatcgcaag aaagacaagc atcgttccaa
    22081 agtcatcttg ggccatgatc aatgctaaaa ccggtattgt cacgatcaag agcttcccaa
    22141 tcagccaaaa gtccgatttc atcgtccgtt cttttacttg cgtattatgt ttagttacga
    22201 tcagtgccat catcaagatg tacgcaatct tcatcaactc tgctggctgg aaagtaaacg
    22261 caccgaatcg aaaccagttt ttagaccctg ttgaagcagc taaattctga tcatagaatt
    22321 tgagcaagag tcccattacc actaacccca aaccataaag gtaaggagtc agtttccaga
    22381 tccatttgga attcaaatgc atgatgacaa cgattgctgc cgctccaact aaataccaga
    22441 caacttgcat ccctacgcct ttcaagactg ctggattgtt cggatcatgg ctaagtgcta
    22501 catataatga gagcagacca atcaaacata acagaaagac tggtaaaatc acgccataat
    22561 caatacgatt atcgttcgaa gcttttaatt ttttttccat gtttcatccc ttttcttaaa
    22621 aaccatactg ctgattatta tagcggtcca gttagataat ggaaagtctt tccatagaca
    22681 tttgggaaaa attaactttc cgtttgtgca gttgcttgag caaatgcagt acaaaagcct
    22741 actgcttatc tttagtgtat catagaactt tttttgatac atgcaattat ctaccagctg
    22801 agattctcct cgctgtgaga agtttctgtg ctttcattgt atcgaaggat ctcttcggca
    22861 atctctagca gctgatcaga aaccagattg gttcgttgga tgaaataaaa atatcgatgg
    22921 aaattcaacg gagtcattgg cacattttct ggaacagagc gcttagacaa aatcgagcga
    22981 ccgattcctt ccttcagcaa cgcaacaatt acatcgttac ttttcacgat catcttctta
    23041 tcttggatat tcatctcttc aaaataacgc tgcgtgtaat gaaaaacacc agagtttgct
    23101 tctcttacaa gccacaattt gctttttggg tccccagcga tgactagctg gtcttccatc
    23161 aaagagtatc tttgtacgcc ggaagtctct aatggctttt cgataaaacc aaaatcggat
    23221 tcatgtttcg ctacttgttc taatgattca tgagaattcg tgatttcgat ctcaaaatgc
    23281 actttaggaa accttttgat cagatgtttc atcaactgag gcaaaagata gacagcaaag
    23341 gtgtgggaag caacgatccg acatacttct tcttcatttt gttcattgag caatttttgc
    23401 tgagtttctt cccattcttc taacaaaacc agtgtccgtt catataataa gtcggcttgc
    23461 ttagttggaa gcatctcttt tcttccgtta cgttgaaaaa gatcgatatt caattcccgc
    23521 tccaactgtt tgatttgatt cgagactgct ggttgagata aaaacagtac ttctgctgct
    23581 ctggaaaaat ttcttgtttc atatactgct cgaaaagtcg ttaataactt aaacatggca
    23641 ccacttccat aactttttat gataattact ataaaaacta tttgatttaa atatacaaaa
    23701 aaccatctat aatagaaatg tacaacaaaa cagttgctga tccaactagt aacttagatg
    23761 aagacaatca gctgtctgct tgctcgtaat catacattcc tattcatttt acaatgtatt
    23821 cgttcatctt ggtactaaaa tctattaaat aaaaaggaga atattatata tgaagaactt
    23881 tttgaaaatt attccgatcc cgatctgcgg gttgatcctt gggttagctt cccttggaaa
    23941 tctgtttaaa gactatcacc atgttgcttt aggcaatcta actggcggta tctctatgat
    24001 tcttatgata ttcatttttg ttaaattgtt tatcttattc gaacacacga aacaaacgtt
    24061 gcatgatcca atcatcgcct ccgtttcacc aacttttacg atgtcactca tggttatttg
    24121 tacttatttt gtttcatatg aaagtattgc accaattgtc aaattcattt ggctgtgcgc
    24181 tgttatcttc caagtgatcc ttgtctttta tttcaattat caccatgtta tcaaagctga
    24241 tctttcaatt gaagcaattt atccaagctg gtttatcatt tacgttggat ttggggtcat
    24301 cactgtaact gccgggaact tcttcccact tattggaaaa attttctttt ggatttcatt
    24361 gatctgctat gcgttcttat tgccgatcat catcaaacgt gtctttttcg taagaaatat
    24421 ggctcatccg acacgtccgc tgattacgat cgttgcagca cctggttctc tttgcctaac
    24481 aggttatctt aaaaactttg ctgaacctaa tttttatctt gtcgtagcat tgttcgcttt
    24541 atctcaagta ttgtatttcg ttgtgctgtc tatgcttcca cgtttgttca agcttcgttt
    24601 ttatccaagc tatgcagcct ttacattccc gcttgttatc tctgcaacag ctatatttac
    24661 tactgttcat tacttcaaca gtttaggtat ctatagtagc gtattggatt tcttaaaagt
    24721 tgcggaatgt ctgtttgcaa gtgtagttgt cttctacgtt cttgctcatt acgtacgttt
    24781 cttaattaaa gaacatagca aacatggaaa atttgtttct aaaaaaatcg cttaataatc
    24841 gattcaatag gttgtgaaaa agctgttctg catcaagtaa taagaacggc caaacaaaaa
    24901 cagctgttca tgtttttgta ctggttgggc ttattaccgc agatgcaagc ttttgaacac
    24961 cgtttaccaa gaggtcgtaa aagaagtggt cagcttcaag aaataagaag gaatttacga
    25021 aaattgcttc tcaaattttt gtgaaatttc ggcttatttc cgtagaagct acttctggaa
    25081 caccgtttat tcgaaacaag aggtcgtaca agacttttgt cacgacctct tgttcttttg
    25141 tcaaaatact ggtttagtgc tactatgttc gagtatgctt ttagtattta ttgacgaaag
    25201 gaaccgatca aatgaaatca ttgtcacatc caataaaatc tgtaaaggat tcagcggtcc
    25261 gcgaaatctt acccggttta ctgctttctt ttgccatcgc tgccttcagc aggcttcttg
    25321 ctatttatct gccacagtta ggtggagcga ctattgctat tttattgggt attttattag
    25381 gaaatacttt ttttaaacag cctgttctag caatcggcac aaaatttgct gaaagcaaac
    25441 tattagaatt ctctgttttt ttactaggtg caactgtcac agctcaaacg attgctcaaa
    25501 ttggtgtaaa aggaattgtt tttgtgttac tgcaaatgtc agtcactatc atcggtgctt
    25561 acttgattgg taaaaaactg caattcagcg atacttcttc tttgatgatg gctggaggaa
    25621 acgctgtttg tggttcttct gctattgctt caatcgcccc ttccatccat gcgaaagagg
    25681 aagaaaaagg acagatcatc acactagtca atctactggg tactgtgatg atgttgactc
    25741 tcccttttct tggattacag ttgtttggtg gaaatctcat tgaaaaaagt gcacttttgg
    25801 gtggtacact ccaatctgtt ggtcaagtag tcgctggggc aagcttactt gacaattctg
    25861 ttgtcgaatt cgccatgcta tttaagattc tacgtattat gatgctcgtt gttgttgtct
    25921 atctgtttga aaaattcatg cacaagaaac aaccagctct gtttgaaacg ccaactgata
    25981 cgcagaaaaa acgaaaatta ccgcttcctt ggtatgtatt agggtttgtg atcttttgtt
    26041 tggtcaacag cttgttttct ttacctgtag ttattgacca atcggcacat tttatcagca
    26101 gctggtttga gatcactgct ttagcggcaa ttggtctgcg tttagacttt gcaaaattca
    26161 tgaaagaagg tacacgcttc ttatcttatg gattagctgt cgggcttctc caaacagtga
    26221 tggctttact atttatttgg ctgatcaaag tttagaatga acatgaaaaa acagttgtga
    26281 ttctcgcatg gaacaatgtc cgaatcacaa ctgtttttaa ttttgcagat atccttctaa
    26341 caacaacaat tggcgtttga tttcaacccc atctattgcg tttcccatat atcctgtcag
    26401 ttttccattt tgcccgatca ctcgatgaca aggaataacg attggcaatg gattggttcg
    26461 atttgcctgc ccaatcgctc gcacagcttt cggactatca acagcaacgg ctacttcttt
    26521 atacgttcgg atttctccat aaggaatggt cgacaaagct ttccagacat tttgctgaaa
    26581 aggcgtacct ctctttatag ataaaggaac atcaaattct tttctttttc ccgcaaaata
    26641 ttcttcaaac tgttcttgtg tgcgcgttaa aattcgctga ttcgctgatt cgctttctga
    26701 aattggctga aaagatacag cagtcaatcc ggattcatct gcatctaacc ataaaaagcc
    26761 gataggtgtg tgtattttca ttttgtttgc tcctagtcaa tagactggaa cttcctcctt
    26821 tttctctatc caaaactact tgccaactaa taaaaaatgt tcttaaaagt ttgataaacc
    26881 gaggaaacat ggtaaactaa atacgttagt ttacaaaaag aatacctgtt cttttacgta
    26941 aaatcaaatt ttaaaggtgt tatttaaggt ttattaaatt ttgaatgtaa accatatata
    27001 aacatacgat tgaaaggata gaaacgatga aagtatcaat tgttgtccca tgttttaatg
    27061 aagaaaaaac tgttcctctt ttttttgatg aggttgaaaa attcaaaggt gcccatgagt
    27121 tcgaatattt gtttattgac gacggatcaa aagacggtac gttagcagca atcaagaagc
    27181 tagcctcagc ttatccttat gtccgctttg tctcattttc cagaaatttt gggaaagaag
    27241 cggctctgta cgctggattg caagcttcta caggagattt agtgaccgtg atggatgttg
    27301 atctgcaaga tccgcccgaa ctgcttcctg agatgatcaa ccggatcact acttctgata
    27361 tcgattgtgt cggcacccgg cgttcgactc gtgaaggcga gcctgcgata cgcagtttct
    27421 ttgcgaaaaa attctaccag ttgatcaata aaatcagcga tacagaaatg gtcgatggcg
    27481 ctcgtgattt ccgcttgatg actcgtcaaa tggtggatgc cgtcctagaa ttaacagaat
    27541 acaaccgatt ttcaaaagga ttatttagct gggtcggatt taaaacagaa tatatttctt
    27601 atgaaaaccg cgaacgtgtg gctggcgaaa catcttggtc cttctggaaa ttattcaact
    27661 actcgattga tggtatcgtg aacttctcgg atgcaccgtt aacgattgct tcatttatcg
    27721 gcgctttgtc ttgtgtcggt tctggtattg caattttgtt cattattttg cgggcattgt
    27781 tgtttggtga tcctacatca ggatggcctt ccatggtctc gatcttcctg ttcattggtg
    27841 gaatccaatt gttatgtctt ggaatcatcg ggaaatatat tgggaaaatc ttcttagaaa
    27901 cgaaacagcg tcctgtttat atcataagag aaacagaaaa agtgaagcca aatgaattga
    27961 agcctcacca ataatatacg tcaaatgaag taaacatttt tttaaatgaa aaaggactgt
    28021 gacaaaagtc ttgtcacagt ccttttttcc gaataaatgg tgttccagaa gtagcttctt
    28081 cggaaataag ccgaaatttc acaaaaattt gagaagcaat tttcgtaaat tccttcttat
    28141 ttcttgaagc tgaccacttc tatcacaacc tctttcatat cttattactt cttcattgct
    28201 gcttttagtg ctgctgctaa tgggctttct tctggttctt ctgcttgtgc atattttttc
    28261 agcaagcgtc gttcttcatg tttggtcatt ttcttcttct gctctttttt atcaagcaat
    28321 ttttctgtcg ttccatcgta tttacaacgg aaataagcac cgtttttacc ttcgatgatt
    28381 tccattttcc ggtgacattg cgggcatcga tgattagaga cttttggatc ttttcttctg
    28441 cggtagttgc aatctgaatt ggtgcagaca tagattttgc cgtcacgtgt gtttttctcg
    28501 cgcagattgg ccccacattc tgggcatttc ttttgggtaa tcgagaaatc ttgatattct
    28561 tgtttgcttg atttgatctc attcaccaag cgttttgttt ctttttcgat tccattcaag
    28621 aaggttcctg cttgtttctt tcctgcagca atagcttcaa ggtctttttc ccagctttct
    28681 gtcaaatctg gtgttactaa agagggattt accaatacta ataattgctt acctttagga
    28741 gatacttgca gtccttgagg ggtacgttcc atcagttcag atttgattag tttctcgata
    28801 atctctgcac gcgtagctgg ggttcccaga ctgtattttt ccatttgacc aagcaagctt
    28861 gcttctgaca atggtttagg cggtgcagat agttcttttt tgatggcgaa atcgggattg
    28921 attctcattc ctttttccca tttgactgtt tccgtctcag tttcagtttc gcctttccat
    28981 cctgcttgga cgacacgatt ttgccgaaaa atgaatcgtt ctttaccaaa ttcaaccgta
    29041 acttttgttt ggctgacttt atggggttga gcaaacagtc ctaaaaaacg agagacaatc
    29101 atttgataga tttttcgctc ttcattactt aatttttcat aacgtgctct gttttcagtt
    29161 ggcaaaagtg catggtgatc ggtcacttta ctgtcttgga atacttttgt ctgttggacg
    29221 atggctccgt ttttcaaata ctgcttgacc tcaggagcaa agtcgctgac tgcttgcaat
    29281 cgttctttca tcgtactttt catgtcattt gtcagatatt tgctgtctgt acgcggataa
    29341 gtaacgatct tatgggtctc atacaaactt tgtacaagac ttaatgtttt ctttgcagaa
    29401 tagccatagc gttgattggc ttctctttgg atctctgtta aatcataagg caatggcgca
    29461 gattccgttt ttaatttttc ttggacttct gtgacagttc ctttgcttgt actgatcaat
    29521 tgaacaagtt tttgtgcttc ttcttgtgtt tttaaagcaa attgattttt ttgaagcatt
    29581 ttggcttgtt cttttccagc ttgcaacgtg atcgtgaaat acgtttgtgg acggaactgc
    29641 tcgattttct tttcttggtc tctcactaat gctagtgtcg gtgtctgcac acggcctgct
    29701 gataaattat ccgcgtactt cacagttaag gctctggtca cattcaatcc taccagccag
    29761 tccgcttttg cccgggcaag agcagattca tacaatgcat catattcttt cgcaggtctt
    29821 agttgcttga atccagtttt gattgcttta tctgtttgag aagagatcca taaccgcttc
    29881 actggtttat tgaaatgcac atactctaag atccagcgag caacaagttc cccttctctt
    29941 ccggcatctg tcgcaatgac tgcttctgag acatcttttc gctgagccag ctgcttgatt
    30001 gcttttaatt gatgtcctgt tttaggaagt ggtttgattc ctagtttttt agggatcatt
    30061 ggcaacgtgt ccatttgcca gctctgccat tccttattca gatcttccgg cattttcaat
    30121 cctaataaat gaccaagggc ccaagtaacg atcaccttgg gtccttcata ataatttttg
    30181 tgcttttgat tagcaccaag caccttgctt aggtctttcg caacgcttgg tttttccgct
    30241 agaattagtt gtttcataaa attcctttct gctttctact gtgatgggct gatgattttt
    30301 caacaacgca agtcccttgt attgctgtaa atcatcgtca cactcttcac accagcgctc
    30361 atctccgtca ttccaaaagg cgctgagaaa gttacttttt gcattgatat attcataatt
    30421 ttgatggatt tcgttccatt gtttttcgta cgcttgtcgt gcttcttcga aagactcgaa
    30481 tgtttcttct tcttcaatat cttcttccca gtcttcaaaa aaccaccaag gctcattgtc
    30541 cccgaacatg gtaattactt gatacatact cgtgctcctt ctttcagctt ttctagactc
    30601 atcttagtca cttttcaaga aagttgcaag aattttctct tataaaaggg tcatttaatg
    30661 aacatcttaa aattgttaca aatgaatctg attgtttaag gcaaagtagc gataaagtta
    30721 ttgatcatcc aaaaatagct gtcttctgga tcgattgttg gttcttttgt aaagtatcct
    30781 aaaaaacgta aggaaagata accgtggatc atactacgaa gttccctgct tcgatgaact
    30841 ttttcttctt tcgttaattg aaaagcctca aatatctgca aaatgatctg attcgtttca
    30901 tggattccat ctaataaaac agcttcattg gtatgcggta cactgatcag taactcatac
    30961 actgcttgat ttgaaaaagc aaaggatcga taagtttctg catagatctg cagtgcttcc
    31021 tgaccacttt ttcccacaag agcccgtcgc agctggtcat taagttcctg cagaagtttc
    31081 gctgttagcg ctgttttgac atcacgaata tttttgaaat ggttatacag cgagggagcc
    31141 ttgatatcca agcgtttggc taaatgctga aaggttattt gctgaacatc catctcttct
    31201 gctagttctc gaaagcagtc gatgattttc tcttgagaca gatttctttt tttcatttcc
    31261 atgtttcctc tcataaaata aatccttcac tctttcagtt taccacgaaa aagaaagaga
    31321 aaaaagagac tttcattgat aaactacata ttgtagttta taataaagag ctgtagatga
    31381 aaattttcag aaagaaggag tctaattggc tgcaagaata aaggaaaaat tatttaggaa
    31441 aagagaaact tgggaacaaa acttgtttgt cttatggttt ggaacattta tggctggtat
    31501 cggatttagt ttagtcatgc cttttatgtc actttacata gatacgttag gtaattatag
    31561 tacgtcacaa ttgaactttt ggagcggatt gactttttca tctactttct tggtgactac
    31621 gcttatctct ccttggtggg gaaaattagc cgaccaaaaa ggaagaaaat tgatgctgtt
    31681 gcgtgcttca ttaggaatgg caatcgtcat ttctttgatg ggttgcgtga ctagtgtgta
    31741 tcaattagtc gcattgcgtc tgcttcaagg gattttttct ggttatatca gtaatgccac
    31801 tgcccttgtc gcaactggga cgccaaaaga aaaaagtggt caagttcttg gaaccttagc
    31861 aactggttct gttacaggaa cattacttgg accgcttctt ggcgggatct ctgcttcagc
    31921 gtttggctat cgccctacct ttttcattac agggatcatc ttatttttcg tcttcttgtt
    31981 gagtctgttt ttcgtccatg aaaaatttgt cccagtgaaa aaagaaaaca tggtttctgc
    32041 caaacagatt ttcaaagact tgaaatatcc acacgtcgtc atcggtatgt tcgtcacaac
    32101 gatgatcatc caagcatcca acaattccat tagtccaatc atcagtctgt acattcgcca
    32161 attgctgcat ggacatggga atgttacgtt gatcagcgga atcattgctt cgatacctgg
    32221 gattgccact ttgatagccg ctccaagatt tggtcgctta ggtgacaaga ttgggagtga
    32281 aagaatactt gcgattggtt tgggttttgc gattctggtt tatatcccaa tggcattcgt
    32341 cacgaatgtt tggcaattag ctgccttacg ttttttgatt ggtatctctg atgcttgtct
    32401 gctccctgct gtccaagcat tgatcacaaa atattcgcct catcaagctg ctggacggat
    32461 cttcagttat aaccaatctt tccaagcaac tggtaacgta gtagggccta tgatcggctc
    32521 cagtgtttcc agtgcatttg gttatcgtgg cgtctttctt tcgacttctc ttcttgtttt
    32581 ggtcaacttc ttgcttgtac gacacaatac acaagagatc catcagcaag aaactgcaga
    32641 agaaaaagct tcaggagtct ccatagcttc agggcactct ccttctcata gacaataagc
    32701 gtaaatgaaa aacactgctt gaacagttgt cacagactgt ccttgcagtg tttttgttta
    32761 ggcctgtttt ttcaagaact cagataagag gaataatccc ttttccaagg tttctttttt
    32821 acaacagtaa cctagtcggg catgcttcgg taaatcaaag gcacttcctg gtactaatag
    32881 tactcctgtt tcttctaata aattgataca aaatgtctgg tcatcttctt cgatggctaa
    32941 tttaatgaaa gaagtagaga cgtaattggg agcgacaagt tcaactttcg gttcattggc
    33001 tacccattgt tttaaaatca caaggttctc tgtaatgatc tttttgtttc gcgccaatat
    33061 tttttcttta ttcttcaatg catgtacagc aagatcatct gacaggactc cgccgcaaat
    33121 catcgtgtag tctcgatact ttcgaaaaac ttctgccaac tcaggtccag tagctgtcca
    33181 gccaatacga atacctggaa tggagtaggt tttggatagg gaattggttg ctataccttt
    33241 ttcatagaca tctacgatcg acaagaattc acctttatcc gttaaaggcg catatacttc
    33301 atctatcaat acatatgcat ctactgtacg agcaatttct gcgatttttt ctaaagtttt
    33361 ccgatctagc aatgtgcctg taggattgtt tgcactattc aaacagatga tttttgtttc
    33421 aggtttgact aaggcttcta gctgttctaa atcaaactgc caatcttctt cttccttcaa
    33481 atgaacaaag tcaacagcgg cacctaaaga tttggggatg tcatataatt gctgataagt
    33541 aggaagcatc gaaacaacat ggtctcctgc ttccactaac gcgtatagag ctaaaagatt
    33601 agcaccagtc gctccattcg tttgtaagat gttttccgga tcgacggttt gataaagaga
    33661 agcgacttct tgtttgaaaa catctgatcc ttcgatccag ccatagtcca atggttcgtt
    33721 tttgcgtttt tcgaaaaatt tacttacgct agtcccatct aagccaacga gttcgtctaa
    33781 tgtcaatgct tcgatcgagc tttgtgcaat atcatatacc gctttctttt cccatttatt
    33841 tagccattct tctacaccga aatttgcgat tttcatgttt ctttccccct tatatcttga
    33901 tccagcctac agcaagcaac ccgattgaaa gcagtgcggc cacacaaatc agcccaatca
    33961 ttactttttc ttttcccgta atttgatgtt tggtttcacg acaagcgatc cagtagaagc
    34021 aaaaaccagg aagataggca atactacata aaaggacata agaaaatcct gctaaaatga
    34081 tgcccatcaa ttcaaaaact acagcaatcc caccgatcac tacttgtttt ttgattcctt
    34141 cttttaaaga aaactggact tgatagatcc cgacaagtag ataacaaatc aaaattgccg
    34201 ctgtacataa agaataggca aattgatagg cataatcagt aactaagaaa gtcaataaga
    34261 atagattagt caagattgca gtaacaacta acgaataagt tggggcattt tttttattcg
    34321 tttttcccca acctttcggc aacagaccgt ctcgagccat caacatcgtt gtttctgctg
    34381 ggagcatcgt ccaagacaac caagaaccaa tgatcgaaat aatcaagccg atattgatca
    34441 gcgctgctcc ccaatttccc acgattcctt ttaaaatatt tgccatagca ggttgtgaaa
    34501 tagttgctaa ctcttcttga gataagacgc cgtatggcaa gatcgaagca aggatataga
    34561 taaacaataa acaaagcaga ccaatgatcg atgcttgctg ggcttctgaa cgtttcttcg
    34621 cgcggttggc taaaacactt gcgccttcga tccctacaaa gacccacatc ataaccatca
    34681 aacaaccttt aatctgcaac cagatcgttc cttgctgccc tgttccattc actagattat
    34741 cagatacgtt accccaaaaa tcagcagtga atatattcac tttaaatgcg ataaatacaa
    34801 atattaaaaa taggaataat ggcactaatt tacagatcgt cacgattgta ttgatgacgc
    34861 tcgctccttc aactccattg tttactaata aagtcagacc ccaaataaaa atacttgcaa
    34921 ataaaataga aggaacattt tgtccacctt tgaatatcgg gaaaaattct cccatggcgc
    34981 tcatcagcat cgttgcaaaa gcaacatttc ccagccacgc agacaaccag taaccccaac
    35041 cactgatgaa gccacctaat ttaccaaatg aagcttccgc gtaaccgaaa ataccaccat
    35101 ctagatctgg acgcttttct cctaaatgat tcagcgataa taccaaagct aaaataccaa
    35161 tcccaacgat tatccatgaa aggatcgctg gccctggagc tgcagaacta gccagatcac
    35221 ttgtgatccc aaacacaccg cttccgattg acgaactgac aaccagagca accagtcccc
    35281 atttgctaat acccgtcgat ttcttttcca tttccatcac cctcacttat aaaattgaac
    35341 aaatcacttg caaaagattc ccttctattt tgcaactgct cttgcttaac aaaaagcaac
    35401 tttattcata ttattggata ttatgaactt tttacacaag tatttcaagt ataaaatgaa
    35461 taaaaattaa ttttattgca aaaaaacagc tatgctatcc aaaagaaaag gtgtttcttt
    35521 tgttagccat agctgttttg attcatttaa tatcgcttaa acgtattgta aaacttttgt
    35581 ttcttgatat gcgtggtcga tcaaaccacc acccagacat tccattccat cataaaatac
    35641 aactgcttgt ccaggagtga ttgcacgtac aggttcgtcg aatacgactt ctgcacgatt
    35701 atcatccaat aagcgaactg tcacaggaac gtcttgctga cgataacgga atttcgctgt
    35761 acacttgaat tcttttggca ttggttcatt cgtcgtaaag tggatttcac ttgcatccaa
    35821 gcttgtggca tacaatgcag gatgatggaa cccttgcccc acatataatg tgtttgtcgc
    35881 tagatctttt cctacaacaa accagggttc ttgcgaatct cctccgccac cgatccctag
    35941 tccttgacgt tgaccaatcg tataatacat caagccagca tgttgtcctt tgacttcacc
    36001 atcaagagtg accatatttc ctttttttgc tggcagatag ttgctcaaaa attgtttgaa
    36061 gttcttttca ccaataaagc agattcccgt tgaatctttt ttcttagcag tcgcaaggcc
    36121 tgcgcgttca gcaattgccc gtacttctga tttttccatg ccgcctagag ggaacatcgt
    36181 ttttgaaagc tgttcttgtg ataattggct aaggaagtaa gtttgatctt tattattgtc
    36241 tactccacga agcatgtgga ccacgccgtt ttcatcgcga gtgacttgcg cataatgtcc
    36301 agtagctaca taatcggcgc caagatccat tgcataatcc aagaatgctt tgaatttgat
    36361 ttctttgttg cacatcacgt ctgggttagg tgtacgtcct gcgcggtatt cggctaggaa
    36421 atattcaaat acacgatccc aatattcttt ttcaaaattc acagagtaat aaggaatccc
    36481 gatttgagca gctactttcg ctacatcctt ataatcttcc gtggccgtgc atacaccgtt
    36541 ttcgtccgtg tcatcccaat ttttcatgaa tattccgatc acgtcgtatc cttgttcctt
    36601 taacaaaagt gctgttacag atgagtcgac accgccgctc atccctacga caacgcgtgt
    36661 tttgctgttg tctgtcatga tatcaccatc atttcaatta gattctgaaa catctacgag
    36721 ttgaaggtcg caacttttca gtattttaca ttatacgtca tatttctgaa aatttcatcc
    36781 cttaattatc aagtcgtaaa aatactgttc tactggacct attcagtaca caaacatgat
    36841 agtcaaccaa aaaatcactc atcttttgag cattcgatat aaaaaattgg catactcata
    36901 aaaagggcca gcctttcggc aataaggaat aattttataa attgtgaaaa actatttttc
    36961 aaaattgctt cttatcgctt aaagctgcac gtctcattta caaccttttc tttttagatg
    37021 ttatgagttg ctcagataag tgattttgtt tactctaaga aaaaagggag gcaaaagata
    37081 catgaattac tttatcagtg atatgcactt tttccatgaa cgcttattag gaaaaaacga
    37141 ctttgctccg cggctctttg caaatgtgga tgaaatgaac caacagttga tcactagctg
    37201 gaatgcagta ataaaagaaa ccgaccatgt ttatcatcta ggcgaccttg ctatgcatcc
    37261 gcaatatgaa aaagcaaatg cagaaatttt ggaactggtc actcaactgc aaggaacaat
    37321 ccacttgatc aaagggaacc atgacagtcg cgcttttttc cgttatttag aaaaaaatga
    37381 tcctaggctt actgacggaa gtcccaaatt ttatttttat gatgtaggtg ccatcgttaa
    37441 attcgaccat caacaatatt acttgactca ttatccgatg ttgctcgggc ctaacgacac
    37501 tatccgcaat cttcatggac atatccatca ttacagcgta cctatcgaga acgatgtcaa
    37561 tgtaggcgta gacgcaccgg aaagagaact cttaaaggaa aaactaccat ttggcactcc
    37621 gttatctgag caacaaatca aagaaatcta tgaagcaaag aggaagtacg ataggtcgtg
    37681 aaaaaactgc cctatatcaa gtattaaaca aataaagata attactcata tttttcattt
    37741 gagtaggctt attgccacag gaacattagg atcagaaatg gagttgtaac aagacttctg
    37801 tcacaactcc attccatact attcgtcttt ttgctcatgg tgttctttta ccagtttttt
    37861 ctgtaattct ttatcataat ttcgggcagc tgacgttaaa aataaaacga ttccgcataa
    37921 taaaatcccg attccggaaa acaagaacat tttttctacc cctaaacgat cagctaatgg
    37981 tcctgcaaat aataatccaa ttggacctgt caggttcatc aaagagttga agttccctaa
    38041 gacacgacct aaaacattcg attcataact ctgctggatc attgccatca gcagtgtatt
    38101 gaaatatggc gttgcacaac ctgctcctgc attcaagata agaaagtaca agaacccttg
    38161 actatcgttt ggaaccaatc cgcttgcacc gatcgtcagg cccataatga tgtaggctat
    38221 aataataggt ttcattcggt ctttccatgt tcctataaaa ctgatcagtg caccgcctaa
    38281 gagcattccg actgcgtaga ctacttccac tgctcccgcc tgtccaacgg aaccaccaaa
    38341 gtaatctagc gtcatcaatg gatacagact gattgctggc atgaacaaca gcatcacaaa
    38401 tgcaccattg atcgttatgt accataaccc tttattatcc atcaattgct gcagcccaaa
    38461 ttttgcatct ttaaaaatag taagcggaac ttccaatatt tcaggagaaa cttttgggat
    38521 cttcacaaaa atcaataagc cgacaccaaa aactgccccc aatacatcta ataaaataag
    38581 gtaattgaca ggaacgactg aaaacaaagc cgcacctaga gctggcgcaa tgatgaaatt
    38641 cgctgattgg accattccta actgtccatt cgtccttgtt atgtgagaag atggaacgat
    38701 tgtcggcata atcgactgga tcgttggcat ttggaatgtt tgtgcgaccg aacgcataaa
    38761 caaagacaca aaaactagcc aaatcgggaa ggattctgaa atcgttccga ctactgctaa
    38821 aataagcgcg aaaatcgcta caataatatc cgtgacaatc aaaagagctt ttttatccca
    38881 gcgatccact agcggtccta caaatgggct tagtaaaacc atgggaatca ttcctagtaa
    38941 agtagcaaaa ctcaaaattg tcgccgaacc ggtttctctt gtcaaatacc agatgatcgc
    39001 atattggacg accatactag tgattcctga caaaaactgt cccgataaaa agaacaaata
    39061 atttctcttc caattcgtta aaacgaccga ctgttcattt tccataaaca tccctctttt
    39121 ctttttcatt ttgtatagcg tttgcacaac aatcattata cgggattctt ttttcaaaaa
    39181 ataccttttt ccctaaataa caatcgtttc aatttaaatt tctttactta gaaacaataa
    39241 atcagcaaaa tattaaaaat aacaacatgt gttaaattta aaaataaaaa agacaactaa
    39301 tttgcgtcgt ccttacaaag ttagtcatct aacaataggc cgtttcaaaa ctagttgtct
    39361 ttttatatcc acagatcatt cgactttttc gaataaccca cggctaatca tcccatccat
    39421 cagaaaataa aatttatctt ggctcatttt ctgttttgga tctttgaaaa tattgactgc
    39481 tttcaatcca tttgctgtag tgatcgacat tttcaatgtt tgaatatctt ttgatacagt
    39541 gatttttaat ttgaatcctt ctttgctttg cggcgtggca tctaatggtc ggatcaattg
    39601 ataattgcct ccgtttgttt ctgtaaatcc attatcgcgc aatgtatatc tttttgcatc
    39661 aggatgcaaa cgatagctaa ttggtgcgcc cagtaccgtt gctgttgttt caaatgccat
    39721 gattttcacc tcgataaaat tagtttctct ttattataaa agtttatcct gatttttact
    39781 aggtttttca gttaagttcc ctaaaaaatg aaggcgttct ctcagacatc tgttcatata
    39841 agaatgactt cactagttat tttttgacag cacgaactaa ttgatcaagg aaatactgga
    39901 tgtcgtcttc ggtatttccc aaaccaaagc tgatacggat ggattctttt agtatcggtg
    39961 tatcttctcc atacattgct tccaatacgt gtgaaggctc gatatttcct gctgtacaag
    40021 ctgaaccagt agaaatagcg accccattaa gatcaagctt catcagtagc aaatcatttg
    40081 cttcgccaat taagcgaaga ttcaacacat gagggagttt attcgttaaa tcaccattta
    40141 cttgataagg gatttcgttt gcttctaatg tatcaagaat caattgctgg aatcctttat
    40201 atttgttgtt acgtgcttca cgttcttcag gtgacagcag ttcaactgct tttgccatac
    40261 cccagatccc tgctaaattt tctgtacctg cacgccgctt ttcttcctgc tccccgccta
    40321 aaagcaatgc agggatattt actccgtctc gtttatataa gaaaccgacg ccttttggcc
    40381 cattgatttt atgagcagag atacttaata aatcgattcc taattcttct ggatggatcg
    40441 tttggtttcc gtacgcttgt accgcatctg tatggaaata agctggatga tccgctaaaa
    40501 gttcaccgat ttctttgatt ggcatcaaat ttccgatttc attgttcccg tacatcacag
    40561 agaccaagat cgtatcttct cgtaacgctt ttttgacctc ttcgatcgac agattccctt
    40621 tttcatctac aggcagatat gtaacgtcaa acccctttgt ctctaaatat ttcatggtat
    40681 taagaacagc aggatgttcg atagctgtag tgattagatg tctgccttct ttttgacgcg
    40741 agaacgctgt ttctaagata gctgtattat ccccttctgt tcccccactg ttaaagatga
    40801 tctcgtgagg ttttgcttgc agactgtctg caatgatttg tcttgctgtt tccaattttt
    40861 catgcgcgtg gcgaccaaat ccatgtatgc ttgaaggatt tccaaacgtg tcttgcatga
    40921 ttgccgtcat cgcttcaatt acttgtggat gcatgggggt agttgccgca tgatccagat
    40981 aaatagtttt cacttcattc atcccttcca aattattgct aggcacattc tagcataaaa
    41041 tagcgaacaa accaaagaag agctcatttt tgtctagagg acgtttcatg ggcatagaaa
    41101 aaaggagctg tgacaaaagt cttgtcgcag ctccttgaag ctgctcactt ctgtcacaac
    41161 ttcttt
//
LOCUS       DARBEC010000016        40994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.016, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000016 DARBEC010000000
VERSION     DARBEC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 40994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 40994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.016"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1415)
                     /locus_tag="SCL80_000798"
     CDS             complement(<1..1415)
                     /locus_tag="SCL80_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1106686.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(1426..2094)
                     /locus_tag="SCL80_000799"
     CDS             complement(1426..2094)
                     /locus_tag="SCL80_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEG1106687.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(2224..2808)
                     /locus_tag="SCL80_000800"
     CDS             complement(2224..2808)
                     /locus_tag="SCL80_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1106688.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(3139..3372)
                     /locus_tag="SCL80_000801"
     CDS             complement(3139..3372)
                     /locus_tag="SCL80_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEG1106689.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(3387..4337)
                     /locus_tag="SCL80_000802"
     CDS             complement(3387..4337)
                     /locus_tag="SCL80_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1106690.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(4334..5176)
                     /locus_tag="SCL80_000803"
     CDS             complement(4334..5176)
                     /locus_tag="SCL80_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1106691.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(5178..5897)
                     /locus_tag="SCL80_000804"
     CDS             complement(5178..5897)
                     /locus_tag="SCL80_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1106692.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            6589..6747
                     /locus_tag="SCL80_000805"
     CDS             6589..6747
                     /locus_tag="SCL80_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1106693.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            6819..8045
                     /locus_tag="SCL80_000806"
     CDS             6819..8045
                     /locus_tag="SCL80_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1106694.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(8133..8525)
                     /gene="rpsI"
                     /locus_tag="SCL80_000807"
     CDS             complement(8133..8525)
                     /gene="rpsI"
                     /locus_tag="SCL80_000807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1106695.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(8541..8984)
                     /gene="rplM"
                     /locus_tag="SCL80_000808"
     CDS             complement(8541..8984)
                     /gene="rplM"
                     /locus_tag="SCL80_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEG1106696.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(9503..9970)
                     /locus_tag="SCL80_000809"
     CDS             complement(9503..9970)
                     /locus_tag="SCL80_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEG1106697.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(10090..11628)
                     /locus_tag="SCL80_000810"
     CDS             complement(10090..11628)
                     /locus_tag="SCL80_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEG1106698.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            11708..12394
                     /locus_tag="SCL80_000811"
     CDS             11708..12394
                     /locus_tag="SCL80_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEG1106699.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(12595..13122)
                     /gene="msrA"
                     /locus_tag="SCL80_000812"
     CDS             complement(12595..13122)
                     /gene="msrA"
                     /locus_tag="SCL80_000812"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1106700.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(13206..13883)
                     /gene="pgmB"
                     /locus_tag="SCL80_000813"
     CDS             complement(13206..13883)
                     /gene="pgmB"
                     /locus_tag="SCL80_000813"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEG1106701.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(13880..15010)
                     /locus_tag="SCL80_000814"
     CDS             complement(13880..15010)
                     /locus_tag="SCL80_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1106702.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(15010..17193)
                     /locus_tag="SCL80_000815"
     CDS             complement(15010..17193)
                     /locus_tag="SCL80_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEG1106703.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(17190..18215)
                     /locus_tag="SCL80_000816"
     CDS             complement(17190..18215)
                     /locus_tag="SCL80_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1106704.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(18260..19087)
                     /locus_tag="SCL80_000817"
     CDS             complement(18260..19087)
                     /locus_tag="SCL80_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1106705.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(19084..20151)
                     /locus_tag="SCL80_000818"
     CDS             complement(19084..20151)
                     /locus_tag="SCL80_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEG1106706.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKHGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLIGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(20177..21013)
                     /locus_tag="SCL80_000819"
     CDS             complement(20177..21013)
                     /locus_tag="SCL80_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1106707.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(21010..21879)
                     /locus_tag="SCL80_000820"
     CDS             complement(21010..21879)
                     /locus_tag="SCL80_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106708.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(21949..23232)
                     /locus_tag="SCL80_000821"
     CDS             complement(21949..23232)
                     /locus_tag="SCL80_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1106709.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(23246..24931)
                     /locus_tag="SCL80_000822"
     CDS             complement(23246..24931)
                     /locus_tag="SCL80_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEG1106710.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            25206..26231
                     /locus_tag="SCL80_000823"
     CDS             25206..26231
                     /locus_tag="SCL80_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1106711.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(26826..30500)
                     /gene="rpoC"
                     /locus_tag="SCL80_000824"
     CDS             complement(26826..30500)
                     /gene="rpoC"
                     /locus_tag="SCL80_000824"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEG1106712.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVTETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(30558..34175)
                     /gene="rpoB"
                     /locus_tag="SCL80_000825"
     CDS             complement(30558..34175)
                     /gene="rpoB"
                     /locus_tag="SCL80_000825"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1106713.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLSALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            34903..35871
                     /locus_tag="SCL80_000826"
     CDS             34903..35871
                     /locus_tag="SCL80_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1106714.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            35889..36794
                     /locus_tag="SCL80_000827"
     CDS             35889..36794
                     /locus_tag="SCL80_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106715.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGGAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            36791..37597
                     /locus_tag="SCL80_000828"
     CDS             36791..37597
                     /locus_tag="SCL80_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1106716.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            37733..38722
                     /locus_tag="SCL80_000829"
     CDS             37733..38722
                     /locus_tag="SCL80_000829"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEG1106717.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMLTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(39039..39563)
                     /locus_tag="SCL80_000830"
     CDS             complement(39039..39563)
                     /locus_tag="SCL80_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEG1106718.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            39850..>40994
                     /gene="scm"
                     /locus_tag="SCL80_000831"
     CDS             39850..>40994
                     /gene="scm"
                     /locus_tag="SCL80_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1106719.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDT"
BASE COUNT        12068 a         8467 c         7266 g        13193 t
ORIGIN      
        1 tatttcgtta tgtcattaaa aatctgttct atcaaagttg aatcttctac tctaagtttt
       61 atatcttttt ttattccacc attttttaaa taaataattc tattgcatgt tttataaaga
      121 aattccggat catgagtagc tataactact gcaccctttt cttttaagtc tttaataagg
      181 tcagcaatcc tttccatatt aatataatcc aacccacttg taggttcatc aaatagaaat
      241 aaattagttt ttgacaatga agctatagaa accaataatc tttgtttttc tccaccggat
      301 aaatcaaacg gtaattgatc tttcatttta taaagttcca ttttttttaa aattttttct
      361 tgctgatctt tactaactat ttcattcccc agggctaact cgctcctcac tgattctgtg
      421 aaaaattggt aatccatttc ttgcattact aaaaacggcg atgacaaatt ttttttattt
      481 tcgattatct ttcctttggt agggcgaata atcttcatga gtattcttag taaggttgtt
      541 ttccccacac cgtttttccc taccaaacca ataacatctc caactttgac atcaaaattt
      601 atgtctttta gaatattttc ataagtaatt tcttcaactt ttaatacatc tgcacttacc
      661 ttttcgatat agggaacttc aatcgaacta ttaaaaatgt caaatttcct taatgcgtta
      721 ttgttttcag tttttaacaa ctgaatagtt gtagttttta cagttctatt tttaacaaat
      781 agaacttgat caattaattc tttcaaatag taaaaacgat gttcagctat aacaatagta
      841 aatcctttat tttttaaatc acaaagtaag tcagcaagtt tcttagtcgc tttgaagtcc
      901 aaatttgaag acggttcatc caatataatt aatttagagt ttagcgataa actacatcca
      961 atagaaattt tctgccgttc accacttgat agcgaaaaaa tgtttttatc taatatattt
     1021 gaaagatcta ataattcagc taattcattg attcttttat ccatatgctc ttttgtaaat
     1081 ccaaagtttt ccatcgaaaa tactaactca ctcgttgtat ttgttgtaaa gaattgtcct
     1141 ctaggatctt gacttacgta gccaatattt tttgatatac tttcttgtgt taaggattgt
     1201 agcgagttat tttcaaataa aatttcccca accaattttc cgtcataaag tcttggaata
     1261 agtccattca ttaactttaa tagtgtggat ttcccactcc cagaagaacc agtaatcaat
     1321 gtaagactgc ctttttttat tgaaaaatta aggtcaacaa gcgcttgttc cttaacatcc
     1381 ttatatgaaa aagaaacatt gttaaattca aacaattctt tcacctcaaa tcaaaatact
     1441 taagaaaaaa atagtaacgg aaaacaggat gatatagtct aataagctca tgtttgtttt
     1501 atagtaagaa cttcttttca caggagattc tatccccttt gttattgcat tagcggtcat
     1561 ttcttcagca atcatagttg cacgtataag aacgggaact aacagaaatt ccaaatattg
     1621 cagaggctga aaaaaagaca agcgaatgtt tctcattttt aaagattctt taatcattct
     1681 tatttcagaa cgataagtcg gaaagaatct gatcactact gtaatactta acattatttt
     1741 tttcggtaaa tgtatttttt ctaaagagga taataattca ttgctagtat agacactaac
     1801 caacaaccat gagatcatta cgaccggcaa cattcgaaat acaatatata aggatgtcgc
     1861 aaaaaatata gtaaaaaact ctatcttgat agcatcaata attttcatta ctaaaaacag
     1921 aactgaataa atcaagatga aataaataca tgtctttatt ttacgaaaca tcaatactaa
     1981 aactgtagca ataatgaaac accccacttg aaatatattt ccctttccta aaaaaataat
     2041 gaccggaaaa aaaatattta aagtaatcaa ggttaacgga ttgatttttg acactctttc
     2101 acctccaaaa aataagatcg cactgttatc ttaagctgat aaaaatgcac cgttattcag
     2161 ataacagtgc gatcttatca aaaagcataa ctgagaacaa ttctcaatgc aagctttttt
     2221 taattaactc agtaattcaa tccacccagg aacagaaaat tgatacaatc ttctggttaa
     2281 tagcatagtc ttttcttttg aatagctatg cttcaatata tcatagaatg tagcgtagaa
     2341 tgagtaaatg tagaagtgga tttcttcttc attaaagact aaattttgat tgatcattcc
     2401 gatagtcttt aacttatcta gacaacagac tgtctttttc gtcacaaatg atgtaatttt
     2461 ttcaacaata tcttgatatt tggttccagc tgattggaat aaaatcaaat cgaatatacg
     2521 tttattctca aaaatataat taataaatgg atctaattca ttgaagttct ctctccaact
     2581 tttctctata tctatatttt ctaagtttaa gaggtatttg gtaaactctt cattatacat
     2641 attaataagc gcattaatat gcggctgaat aatttcttca aataacgctt ccttagaagt
     2701 aaaatgttta taaaaagcac ccgtagttac gcctgcggtc aggcacagtt gccttatctt
     2761 agtttgctga aatccttctt tttcaaaatg gcttagtcca ctttctatta acttttggtg
     2821 agttataata taatctctgg tttctatggg tacttcctcc taataagatg aattaacttt
     2881 attatcttac acaggacttg aagtttcaat cgttaacgtt tattactgaa ctcattcctt
     2941 agagctaaac taatgagaaa gagcagaata actgatacaa aaagcaatcc tacaagaatt
     3001 aatagactca tctaaatcat tcaccttttc taaaataatc cgagaaagat ttaaaaaaat
     3061 tatatccagc tattaggata agtcctatca taatcactcc aatagccatt ttcttatcct
     3121 ccctaaaaat aaagtctttt acttaaaacc ttttttgtaa tcgtcaataa attttttccc
     3181 tttatcagtt aattgaaatc ctatatctaa atagtcctca tatctaagtt tagccagcat
     3241 ctcacttaca gatgctggac taacacttag aacttttgct aaatcattat tttttaattt
     3301 cttttttctt tttgataatt cctgaattgc tagtaaataa attagtatag tagtcgtttt
     3361 aatttgtacc atacctaccc tccaaattat ttcgaaaggc cttcagcaat tttatccagg
     3421 ttccatttca tcatcgcata gtaactatca ccatcttgtc ctttttctgc aattgaatca
     3481 gtaaaaatcg ttgaatagat aggaatattg gtatcttttg atactgtttt cattggtcta
     3541 tcatccacac tgctttctac gaataaggag ggaacttttg ttgtgcgtaa tttttcaact
     3601 aagtgtttta tttgatctgg tgttccttct tcttcagtgt tgatttccca aatgtaagca
     3661 gaaggcacat tatacgcttt cgagaaatat ttaaagcatc cttcacttgt aacaatcatt
     3721 tttttatcat tcggaattga agcaaattta gatttagctt ctttgtctag agaatccaat
     3781 ttttcaatat acttatctag attttcttta tagaattttt tattatcagg atctttttcc
     3841 gctaactgtt tttcaatatt tttagcgtaa ataataccgt tttctaaatt taaccaagca
     3901 tgaggatctt ccttcccttt ctcactctga ccctctaagt aaataacatc tatgccatca
     3961 cttgctgcaa aatagtcttt gttttcctct ttgttcgcat ttttaactaa ttttgtaaac
     4021 caagcatttc ctccagtttc caagttaaca ccgttataaa aaatcaaatc tgcttttgaa
     4081 gttttttgaa catcttcagg caaaggttca tattcgtggg gatctttccc aataggtacg
     4141 atactgtgta aatcaatttt atcttttgct atattttcgg taatatctgc taaaatcgaa
     4201 ttagtaacta ctacttttaa tttttcgttc gaagccactt tatctttgtc tttactattt
     4261 attgaacatg ccccgaatat aaaaagagca atcaaaatcc ccagacttcc cagtactgct
     4321 ttccattttc tcatcataac gatctctcct tctttcttaa aaatttttgt tttggtgaga
     4381 ttagaaaact aattacaaat aagaatgccg ctgttaaaac aatacttgat cctgctgcta
     4441 tgttaaaact ataaccaata aacaatccaa gcaatgatga acttgctccg actgttgcag
     4501 aaataaatat catagttttc aagctatttg catataaata tgctgtagct gctggtgtaa
     4561 ttaacattgc aacaattaat atagttccca cactttgcat agctgttact gctactaaag
     4621 ttaatagaac catcaataga taatgataaa aactgacatt catccctatt gcttttgcca
     4681 ttaatggatc aaaagaagtg attaataatt ctttaaaaaa tagaactact ataaccaaca
     4741 ctaaaaccga aacgccaatt gtaatccact tatcaatatc ttgtacagct aaaatatttc
     4801 caaataaaat atgaaataaa tccgttgagc tatttgcaac tcctataagg ataactccta
     4861 gtgctaaaaa agaactgaaa gtaataccaa ttgctgtatc tccttttatt acactattgc
     4921 tttttataaa agtaataatg atagaagcta atagaccaaa tacaatcgca ccaataaaaa
     4981 aattaatacc taaaatataa gaaattgcta ctcctggcaa aacagcatga gaaatagcat
     5041 cacccataag agacattcct cttaaaataa taaaacaacc tactgttcca gaaacaatcc
     5101 ctataactac agatgtaatt agagcattct gtaaaaagtg aaagtcatac agaccattaa
     5161 taaaattttg aagcatatta ttccccctct ccaataaata tagtatctcc ataggctttt
     5221 tttaaatttt cttcattaaa tacagattct acacttccgt gagcaattag atttcgattc
     5281 aaaagaataa tattatcaaa atacttcttt accttgctta aatcatgatg cacaattagc
     5341 actgtttttc cctgatgttt caattttttt aacgtatcca taattattcg ttcactaact
     5401 aagtctatcc ctacaaatgg ttcatctaaa aatatgaaat ccgcattttg agcaagacat
     5461 cttgctaaaa gtacacgttg aaattgtcct ccagataatt ctcctatttg atgattagaa
     5521 tattttccca tatcgacctt ttccaaagcc tttgaaactc tttgccattc tgcattttta
     5581 attctttgaa aaactttcat tccggcatat gttcccatag atacacattc ttttactttt
     5641 attggaaaag taaaatcaat atcagctttt tgctctacat atgcaatctt caccagtttt
     5701 ttattgatat cttcattatt aatggagacc tctccctgat gagggataat atttaacatt
     5761 gctttaagca atgtggattt tccagcacca tttggaccaa taatacctgt taaagcacct
     5821 tcttgtattt ttaaagaaat ctcgttcaat gctaaaaaat cgttataaca aacattaagc
     5881 tttttcactt ctaacataac tttctccttc taaatgataa tgattatcac gatcatatgg
     5941 tatacaaaaa ttatatttta gtcaacccta aattttatag tttagatttt tttctgttgc
     6001 tgaagattag aatgacaaaa ctatttgaaa ctacgcgtat agtaagtagc tttcaaaata
     6061 tataaaaaag gagcgttctc aaatgagaac atgctcctaa aactacttta ttggcgtatg
     6121 gaaaaattat acttgaagtg gatagcgggt tgaataagtt gataagcatc aaaacattta
     6181 taaaaattat tcaagactat gtctgttcca tatacaaata tgaaaatcag taaactccat
     6241 ttaacttttt tcgtagtgtg tctttttata catgtttatc gttattaata gtgaatagga
     6301 aaaaaactat ttttgatcat tgacaattaa atacaatttt tgaataacga tccctgctat
     6361 tcgagctaga aactcaagta cgccatgacc tgattagcga caaatactta gagatttctt
     6421 tttgttctgg gaaaacaaaa tgccatgaca atagcagcaa ataatgatac ttccgtaagg
     6481 ctcgccgtaa actggggaga ggtgaaattc ctatgtgctt acccaaagca attcaaaaat
     6541 ttcataacta ctattttagg agatgatttt atagaatcca gagtctacct gaataaaaaa
     6601 caaacctgtg catttctagg aatcgataat aatactctag ataagtggat tcgtatatat
     6661 aatttcccag taataagagt tggtggtgta gttcgctttg acgtagaaga tattaaagac
     6721 tttatgaata catttaaaat aacttgaaat tttcttctcg ccgcatatac taaaattagt
     6781 gaaatatact gccttacttt ttttaaggag tgaggatttt gcctacaaag ctttcaaatg
     6841 ggaaatataa aactaatctg agatatccaa aaaggtttaa agaaataaca ggaattgcat
     6901 cagaaaagta tcaaaaaaca tttccaaata ggcaattggc gattaaagcc gaaaatgata
     6961 tgaagaagaa aatagaaaaa gttttgagag aggagaatgc aaactcatta gaattaaaag
     7021 gaaaaataac gtttaaaaaa ttttacgaat ctaaatggct tcctagatat gaattaggtc
     7081 aaacaattag atcaaacagg cctcctagtg atataaccat tagtaataca aaagatatat
     7141 ttcggttgca tattttacct atgtttggtg aatacgcaat gaattatcta aatatcaata
     7201 ctgagataat atcagatgaa ttaaccaaaa aatcgaaaga atatgcaaat ataaaaatca
     7261 taaaaggata tgttcgttcc atgttcgata ttgcagaaat actaaattat atcgaattta
     7321 atcgaactac taaaattatt caaagtatca ctgcacctaa aaaaaatgct ttagaagaaa
     7381 aaagaataca agaaggtaag caagcactat catcaaaaga acttactaat tggatagaag
     7441 cagtaaatga tgattttaat aatcatttac ttacttttca tgattacaca ctttttatga
     7501 ttaccctgta tcttggtgat agaaaaagtg agacttacgc tttgcaatgg aaatatattg
     7561 attttgaaaa acaaacggtc cgtcttaaac atactttaga taaatatcaa agaaaaaagt
     7621 ttacaaaagg tcgaaaagat accgtcattc aagtaccaga agttgtaatg acattgctaa
     7681 gtgaatggaa atcggtacaa gctgatcaat tgttgaaatt aaaaattaag caaacgttag
     7741 atcagtatct ctttacttat actaaaccat ctggagaagt aaattgtcct gttcatgccg
     7801 attacctaaa ctatcgaatc aattcaatta aaagaaggca tcctgactta gttcatttat
     7861 ctccacacaa actccgtcat acttatgcaa ctatagctag acaaggtgga gcagatatga
     7921 atcaaatttc aaatgcttta acacactcag atatttctac aacaaagata tatgtgaaca
     7981 caccagatat agttgataag gctgtttttg aagcttttca gagaggattg aataagtgtg
     8041 attgatatgt gttcttaaat gtgttcttaa aaacaaaaga agcactttcc aaataattga
     8101 aaagtgccag aaacgttgat atataaagga tattaacgtt ttgagaattg tgaagcttta
     8161 cgtgctttct taagacctgg ttttttacgt tcgaccatac gtgcgtcacg agtaagtaag
     8221 ccagcacgtt taagagctaa acggaagtca ggatcaactt ctagcaatgc acgagcgata
     8281 ccatgacgga tagctcctga ttgtccagcg tatcctccgc cgtttacgtt aacaaatacg
     8341 tcatatgcgc cttttgtttc agtaacagcg aaaggttgat tgattacttc acgcaagtca
     8401 gcatgtggaa tgtattcttc aacatctttt ttatttacag taattttgcc agttcctggt
     8461 actagacgta cgcgagcaac tgcgttttta cgacggcctg tgccgatata ttgaacttgt
     8521 gccaatgaaa ttccctccta ttagattaag tttgtgatgt ctaaaacttc tggttgttgt
     8581 gctgcatgtg gatgttctgc tccaccgtat acgttcaatt ttgtaaattg tttgcggcct
     8641 aaagtgtttt taggaagcat accttttaca gaagtttcga tcaaacgacg agaattttta
     8701 gcacgcaatt cgccagcagt gattgatttc aatcctcctg ggtagttgct gtgacggtaa
     8761 taaattttgt cagtcgcttt tttacctgtt aatttcactt tgtctgcatt gatgacgatg
     8821 acgaaatcgc cagtatccaa atgaggtgtg aaagttggtt tatttttacc acgtagtaca
     8881 gatgcaacaa ctgttgaaag acgtcccaat ggaacatcag ttgcgtctac tacataccat
     8941 ttacgttcta cttcgcctgg tttggccata tatgttgtac gcacgttatt ttcctccaat
     9001 ttaagttctg atgtttgaca tacacttcga gtttccgggg ctctttgtgg ggcaaacaat
     9061 accatttaac atattacccg ctaatctagt caatgtcaac gtttttctgc ttgattgaaa
     9121 aatttatatg aaactttact tcttttcttt cttttatata aaagggtcgt ttcctgtcaa
     9181 aatgaaaaac aaaaagaggc tgtgacaaaa gtcttgtcac aaccccttat tttgaataaa
     9241 cggtgttctc ctgccaactc ctcggtcaca agtcacaata tccataaaac ataagaagct
     9301 gttttttaaa attgtttctt gtgactcgga gctgaacggc aggcttacaa cctcttggta
     9361 aacggtgttc aatcagctga ccctcggtat tagctcaaaa aacggcaaaa tatgaggaac
     9421 tattttgacg tttgtttctc taatactcag gtcaaaacgc tgatttcaca acctcttttt
     9481 cggtcaagtt caacaacagt ttttacgcgt cgatctctgg cgctttgccc aaatgagctt
     9541 ggatcatcca gatacgtttt tctgtggctg ttttgaattc gataaagata tcttgtgttg
     9601 gatcgtcgcc ttcttctcca gaaacttcga ttcctttttg gtaaagatct gctagataac
     9661 gatagcctgc aacaagtgtt tccaaacgtt ctggaatagt acgatcccag cggccaattt
     9721 catcagggat ttttgtgtta tctgcaaatt cacgcaatgt agaataaggt gaaccgtcta
     9781 atgtgatcaa gcgttcggat acttcatcta actgagcgtt caaatcatcc atgaactcat
     9841 ccatcattgg gtgcaatgta aggaaacctg gtcctctcat gtaccaatgt gtttggtgaa
     9901 caacaacaga catttggctt agatctgcaa ctaattgatt taatacttct ttggtttgtt
     9961 ggaatttcat ctttcttgcc tcctaacatt ttcttacact taaaataata gtagactttc
    10021 aatgaaaagt acaacaaaat catttacctg agaacgattt tcagttcgct tttttatccg
    10081 taaagccaat catttttcag aatcagctaa ttcttctcgc agttgagcaa attgctgata
    10141 agtctgtgct aaatgctttt gtgtcgaaac aaataatgga tagattttct gatatttttt
    10201 atgatcagtt tcttgtactc caacttgctc tttcaattct atcaattcct gtgcttcact
    10261 gatactttgg atctttccga tgcttctcca gcctaaaatc acagctccta atgcggatgc
    10321 ttctgtgaca gtagggaaaa caagcgggta acccagtaca tcagccatca tctgacgaaa
    10381 aatcggagat tgagcaaacc cgcctgtagc acggatctct gtgagctgat tttcctggca
    10441 aactcctttt aaaatccgat aaaggttgag acagatccct tctagaatcg ccttcatcat
    10501 ttctttttct gtatgttgcc gctgtaaacc gataaaactg ccaaacgcat ctgcgtccca
    10561 aagcggtgct cgctcaccaa gtaaataggg atgaaaaatc agaccattgg cacccgctgg
    10621 cacaaaagcg atagccgaaa gcagtgcatc atatggattt tctccttttt ctttagcaga
    10681 ttgaacgact tcctgtaaaa atgtatgccc tgcccaatca aagatgccag caccattgct
    10741 agtcgctcct cccactaccc aatgtttctg atccagaaca taacaaaacg tctccatttc
    10801 tggatggaac tgaggctgag tagtaataaa acgaagtgct ccgctcgttc ctacggtcaa
    10861 agcggcgatc gtatcaccaa aagcacctaa ccctagatta gaaagcggcc cgtctgcccc
    10921 tcccaaaaca aatgatatat catttggcaa acctagttct gctgctgttt ttgatggtag
    10981 acctgttact tgataggtgg aaggaaagac ttgcggcaat tgctctattc gaatgttcaa
    11041 gtagtcaagc acttcttctg cccattggaa ttcatggata tcaaaatatc cagttcctga
    11101 agctgttccg tagtcgctga tcagttcgcc aaacaatcta taaaagatat agtctttgat
    11161 accgacaaaa taagcagttt ttttgaataa ttcaggttgg ctgttttgca gccacttgat
    11221 tttgttcaat ggtgtcattg gatgaatcgg cgtcccagtc tgccgataaa attctttgcc
    11281 aagcacggta tatttcagtt tttctgcttc gtccgctgct cggttatctg cccaagtgat
    11341 cattcgagat aacggtgttt tctcttcatc caacaatatc aggccatgca tcgcagagga
    11401 gaaggagaca aattgaatga ttgtgtctga aggcaactgt ttaattactt cttttataac
    11461 ggtgcagaca ccttgaaaga tcaattcctg atcttgttct gccatccctt gtgtttcttg
    11521 gatcaaaggg tagcgaaaat aagcggatgc tgcttcttgg gcttcatctg tgaaagcaac
    11581 tgctttggtt tgtgtggttc cgatatctaa tccgattgaa acgttcattt tttcacctca
    11641 aattttatgt atgtacaaaa atttagttta agcttgcgta tcttggtttt gaaaaggggg
    11701 agatggcgtg ttactttatt ttatcattgg ttgctgtatc ggatcatttt tatgtttaac
    11761 cgcccagcgg attccgttag gtcactctat tatttatcct agatctcatt gtgtcaaatg
    11821 ctgtcgttcg ctttcttggt atgaattgat tccgattctc tcgatcatcg tccaacgatt
    11881 tcgctgccgc tattgcaggt gtcggttgcc cttctactat ttgttggcag aagccttatg
    11941 tggtggtctt tttgcctggt tctttacttt ttcttccgca agaaattttt cgacgttcat
    12001 atggatgctt tcggctttgc tattttcgtt gatggattta ttttacttca tggtccattc
    12061 tcacacgcta ttctgttctt gggcaatttt atggattttt tggttgcagg ccggcgtctt
    12121 ccaatggcaa agtgtgctgt taacgtttat cgtaggcggt gtgtgccttc gctatggtca
    12181 atcctatctc ggagctggcg atactctgtt gatgctttct tggagtggtg gcttgtctct
    12241 agaagagttg cttcagatct tgtttcttgc tagtctgttt ggaatatccg ttttttctgt
    12301 ttactttatc tgtcataaaa aaaagttgga aaagctgcct ttcattcctt ttttgagcag
    12361 tgcgttgctg attgtccttc atcttaggta ttgaaaaaag gttgtgacaa acgacttgtc
    12421 acaacctctg atatatagtg ttctcttacc aaatccttgt gacttggagc taggcgacag
    12481 gttcacaacc tctccactaa atggtgttca atcagctgac cctcagtatt agctcaaaat
    12541 gctggtttta caacctctga tatacggtgt tccataagta gctttttcgg cttcttattt
    12601 cttgacgccc caatgtcgac gaaggaaacc tgcacgtcca gaaccttctc gataaagatt
    12661 gtaatggagc ttgtttttct tgtaataatc ctgatggtaa tcttctgcag gataaaattc
    12721 gcttcttggt tcgatttttg tcacgattgg cttatcgaat cgcccgctgt cttgcaatgc
    12781 ttgttttgaa cgctctgcaa tatttcgttc ttcttcatca ttatagtaga tcactggacg
    12841 ataagaatca ccgcgatcag cgaactgtcc taatgcgtca gtcggatcgg tttgctgcca
    12901 atagatttcc accaactgat cataagaaat aacttctgga tcatattcga tctctactgc
    12961 ttctgtatgc cctgttgtgc ctgttgttac ttgttcataa gttggatttg gtacatgccc
    13021 gccagtataa ccggatgtca cagaaatgat ccctggctgc tcttcaaatg gtttgaccat
    13081 acaccaaaag catccgcctg cgaaaactgc tttttttgtc atataaaatc cccctttttt
    13141 ctcatcatac cttgatttcc aaaaggagga taacgttttg cttgtgacag aaaaaacgga
    13201 aactattata tacgtgatac ggcttctgat aaatgccaaa acgaacgtac ttgatcaaaa
    13261 acggttaaat caggaatcac atattcagca gaaggaagat ttatgttttc acctattccc
    13321 aatacatgca tgttaccagc caaagctgct tgacaaccag cttctgaatc ttcaatgacc
    13381 aagcatgcat ttggcggtac attcaattgc tgtgctcctt tgagaaaaac ttctggatca
    13441 ggttttgctt ttgatacttg cgtgccgtca ataaggacat caaaataact tgtcaaattc
    13501 gttttttcta aaatgaggcg tgcatttttg cttgctgatc ccaatccgat cttgatgttt
    13561 gtttttttca agtactcaag aacagctatt gcaccaggca aaacggatgt ttcatccatt
    13621 ttttgaatcg cttgaagata tagattgttt ttttgctcag caaaggctga tttctctttt
    13681 tctgagaatt tctgttccat ttttccaaat gacagcaatc gctctagtga ctcggttctg
    13741 gatattcctt tcagttgctc gttttctttc tcagtgaatg gaatggacag ctggttagct
    13801 aaaaccagcc acgcttcgta atgataacga gctgtgtcta caagaacgcc atctaaatcg
    13861 aatagtgctg ctttgatttt catcgattac ctccttgaaa atgaacgact tgaagtaaag
    13921 atgcgatgtt tttagctgtg cgatgaagat tatcagtggc ttgctgtaaa gcttcttgta
    13981 aaggcacagc tgcaggaagg atcggaaaaa cggccgaaat gccaacatct gctgcttttt
    14041 cgcttccttt accaacactt ccgcaaatag ctatcacagg aacgccctct ggtactcttc
    14101 tagcaatccc aactggcgct tttcctttta gactttgtcc atctagacgt ccttcgccta
    14161 cgattacgag gtctgcttgc tgtacccgtt tatcgaactc cagtaaatcc aatacgtagt
    14221 cgatgccaga gactattttc cctgaagcaa atgctaacag tcctgcaccc attccaccac
    14281 cagcactact gcccgatgcc tcgattactt gaggggcaaa tctcatataa aaatcatgca
    14341 tggcttggtc agccactgct aattcatttt ctttcaagcc tttttgtggt ccgaaaacaa
    14401 aagaagcgcc ttcttttccg cataaaagat tagtaacatc agtaatgaca gtgattttgg
    14461 ctttaatcag ctcaataggc acgttcgttt tatcaactgt agcaatttta cctagatttt
    14521 ctcccagtgc ttctactttt tgattatctt gatcgtaaaa agcataacct agaccttctg
    14581 ccatgccgat gccgccatcg ttggtactac ttccgccaac gccgatgatg atgtgtcgaa
    14641 caccttgttg caacaattgt cgtatcagtt ctcctactcc tcgcgtggat aacctaagtg
    14701 gctgttgttg gtttagtggg acatctttca atccgacgat ttctgccatt tcaaaaatcg
    14761 cagtttgacc gtctgaagcg tactggattt ctttgttttt gccaaatgca ttagtaacga
    14821 ctgctgtttg ccgcttcaac cttaaactgt ctgctaatga tgaaaccgtt ccttctcctc
    14881 catcgcctat aggtagcaga tcatattcag cctgaacaaa aacttcttga aaacctgatt
    14941 gaatggcttg tgctgcctct aaggctgtca gactttcttt gaaagaatct ggcgcaatca
    15001 gaatacgcat tattttcccc tccgttccaa gatcagttct ttttccagct ggtattggtc
    15061 tccattgaca aaaataggta atgtttcttt cgtctctttt tttattacta tttgtttttt
    15121 tgtcaaaaca aatagcaacc tgtgattttt ccaaataaaa ggaaaagaaa ttttttccca
    15181 tgctgaagga agtcttggag aaatagaaag agttccattc gttactgtaa tacctgaaaa
    15241 accaaatata actgctagcc atagtgctcc taatgaagca gcatgaattc cgtcatctga
    15301 tgaatgcgga tctggtccta aatcaatcag gcacgcttct tggaataaat gatacgccca
    15361 ttccatttct tggatacggc aagcctctat ggcgtgaatc gctttactca acgatgagtc
    15421 gtggattgtt ttgttttcat agtaatgcaa attcttagaa acgattggtt gagaaaaata
    15481 ctcaggtaat aagtataaca gcatgacaag atctgcttgc ttgctgactt ggagttcatt
    15541 tacttcgcta cgggcataat caagtaaaat agactggctg ccaggacgtt ctttgtaagt
    15601 ggtcaaatca ataactggct tactgagata cgtgtcatct tgcggaatca gctgttcttt
    15661 gttaggcact ggcaaatata acttatctaa aaaattttga cattctttca aaaaaagcgg
    15721 atcgccagct tgatatttaa cagctaaggc cacattataa tgcgccagat agttcgtata
    15781 tgcattattg tcgatatatt ctgtgtattc atctggacca atgactctgt gtatctctaa
    15841 atgatcatcg acttttacag ctctgctcag ccaaaaagct gctgtttcat gaatgagcgt
    15901 ttttcccttt gtttctaaaa acgattgatc ctctgttgct tggacatact cttgaacggc
    15961 ataagcaagg tctgcactga tatgatgttc tgcggcagct gaagcaactt cttgtcttgt
    16021 tccggttttg atattcaatg cagcaaattt gggtgtctct tcctgaccag tgtgtgcgct
    16081 ttcccatggg aaaagtgcac cttgatagtg ataatctctt gctttttgcc gtgcgccttc
    16141 taatcgatca taacggtaca gcagcaaatt ctttgctact tcaggaaact gatataaaaa
    16201 gaaaggcagg ataaaaattt ccgtatccca gaaaacatgc cctttataac cttcgccagt
    16261 tagtcccttc gcccctacac taacacttgg atcattgcgg ggagtcataa tctgtaaatg
    16321 gtaagcagcg aagtccatcg ctaactgatc gaaagaagaa tcgccttgga tcgttatccg
    16381 atgctgatgc caaaaatctg tccaataaga catcgactgc tctaaaattt ctgtataact
    16441 aacgcttttc tgggcttggg caatactttt cttaaacggg tctgtttgct gaagagaggt
    16501 agagagcacg atgatcttct ccagcgtaaa ggacgtattt tttctgatct tttctgaaaa
    16561 ggaagcggtg atccggcgat tttttgcttg gaatacacct gctttattga atttagctgc
    16621 aatcccaata tcaattttac tttctgttgt ctggtaggat gcactgatca aatcgtcacc
    16681 aaatactcgc agttcttttt caaccaactg ctgtgtacca aaattggttt gctgtccatc
    16741 gatacccgtt ttcacatcta cttggcagtc ttcggtcaac ggcttgatga ccactttcag
    16801 ggctgcctga tggagattat tttgatcagc aacacgacaa gtaatgactt ccactttttt
    16861 tccttgagca gtttcaatga gcgcatagcg tttaagctct cccatttcta aatctagtgc
    16921 ccgctcgtat gcaagaactt tcatcgtctc aagagaaaaa tgttcaccgt cgatttttat
    16981 ttcccatctg atgacatctg ggagattcac taactcggct gattctgctc ctaaaggtcg
    17041 gttatataca ccagctgcaa acattcccct tacttgattc cgatagtctt cctcctggct
    17101 tcctctgata ccaaaaaatc catttccaat ggtcatctga ctcgcatact tattgatata
    17161 ctctaaatca aactgttttt cttctacttt cataaggtaa taacttctcc tgtttctgcc
    17221 gaacgatata aattttccac cagattctgg atgacatatc cttgccagcc atctgcaatc
    17281 atctgagtct ggtcaccgag cgcccgattc acaaaatgag ccatacttag ttcatgtggg
    17341 tcattaactg gcgtggtctt ttgtaaggaa accagctttc catctacatc ttcgaatagt
    17401 tcgcctggat acagattagc acctgcctga tcgccacaaa aagccacatt cagtttgtct
    17461 tctctgatat tcaaagcaaa cgaagtattc agttggatga tcttgccgtt ttctaattcc
    17521 agtgtgccaa aaattgcatc ttctactgtg aatttgcttg gatcccattt cccgaatttc
    17581 cctgaatttt ttttcgtacc aattttttgg aagctgtacg cccgaacgct ttttacttta
    17641 ggaaatccag taatatacat cgctgtatca agcatgtgaa tgccataatc gatcaaaggc
    17701 ccgcctcctt gaagttcttt gtcgataaac gttccccagc ctggaacacc agaccgacga
    17761 agcgcattcg cttctacgta ataaatatcc ccgagaacat caagatgatt cttgatcatt
    17821 gttgcttcgt cagaaaaacg atgctggaat ccataagcca aaatctgccc gtttttatcg
    17881 gctgtctctg ccatctttct tgcttgatca gctgtcatag ctggtggttt ctcgcacatc
    17941 actgaagcat tagcttcgag ggcagccaat acatgttcga agtgaaagcg attgggtgta
    18001 cagacactca cgatatcggg agaaatctca ttcagcattt gtgtaactga tgtatacgca
    18061 tgagaaaatc ctgtacgtcg gcaaaagtct tctgctcgct ggctgactgg atcaaccaca
    18121 gcggccaatt ctaagacatc tgatcttttt tgataataag cagcatgtac tttttctgct
    18181 acttgtccag cgcctatgat tgctactttc ttcatttatt tctctctcct ttgcttattt
    18241 acttattgtt attctttctc tattctttct ctaaatcatt taaatgacgc gctgtttcca
    18301 gacagttttt caagtattgc aatgattcct gataaatttt cacttcattt tctcctcgca
    18361 aacgacattc atataccata taaccttcat actcattttg aagtagcgtt tgaaaatggc
    18421 gagtaaaatc aattgcgcca gatcctggtt ggtagcgatg attatctgct aaatggatat
    18481 gacccacata ctctttttgc gtagtcaatg cttttgaaat atcatcttct tcaatattca
    18541 tatgataaaa atcagcgatg atctgcacat gtttcaattg attagagtca atgtaatgac
    18601 gcgcatccgc taacaaattg atcatatggt cttggtatcg gttcaaaggc tccaaataaa
    18661 taaccgtacc tgtttcttcg gctactttat ctaaataacg aagtgattcg ctgactgctt
    18721 tatagtctcc ttctttgctt cggggtgaaa ccatcggcgg taatcgataa gtgaacattc
    18781 cccaagctgc tgggataacg attcctttgc ctccaacctc gcttagtgca cgcaaaatct
    18841 cagcaatttg acgtacacca ttaagacgcc gttcttcgat aaagtcaccg atccacccat
    18901 cgtagccgtt acaagctgtc actactggga tccctgtttc tttgattgcc tgtttgactg
    18961 tgtcgatatt ttctattaac aacttaccgt cgatttcgta ttcttcaaat ccacagtctt
    19021 tgataaatct aaatttttct aaaatgtctc ttgggaaaaa tgcttgatct tgcgtaccga
    19081 atttcattgt tctcctcctc ggacttgaac gcccattttg atactttctt ctgggtgctg
    19141 gtctacataa cgcatgtaag cttctgcgct ttctgcaaat ggcacaactg gatcaatgat
    19201 ttcttcacaa tccaagtaac cgttcatcaa taattcccaa catgtgtcct cgattcgttt
    19261 acggttccat cttgggtaat ctgggtttgg ttcactagat gctcgagcaa agacgatttt
    19321 tgcattattg aaatgtgctt cgcgaccaag attaaaccca gctggaaaag gtttagcaaa
    19381 ggcaacataa gaaatgatcc cgccatagcc gatccctatt aaagaagctt gtaatgcggc
    19441 agcactgcca ctcgtttcaa taactgaatc tactccacgt ttatctgtca attcctttag
    19501 acgaaaaccg acatctgctt tcataggatc tagcgtgtaa tcggcaccgt gtttttctgc
    19561 taattctcta cgctgcgcta tgggatcgat cccaacgatg gtagtagcac ctgctttttt
    19621 agctaattga atcgcaattt gcccgatagc gcctaaaccg aagattgcta cagcatcacc
    19681 tgctcgaaca tgtgcttccc ggataccacc taaagcgaat tgagcaggat cgtaacatac
    19741 tgcatttttc cattgatcca accgatcaag tttacgtagt ttataattat caacaccgtt
    19801 tgtaatcagc gtttcacgaa tgggtccata agtgcagact aactctcctt tttgatactc
    19861 tgtcaccaaa ctgcccgttt cttcgatcgt accgacaatc atatttccta attgaaattc
    19921 gccaaatacc acaccgcgtt ggcttccttc ttctcttggc aagaataggc gccattcttc
    19981 tgaaaattct tcatcgataa atggacttaa tcctctaaag tcgacaattt ctgttcctgg
    20041 ttttggagta gcgaaaatta ctcggatacg tacttcgttc ttttcgatct cacggtcttc
    20101 gtaatctttg aatccggcta ttcttggttc taatgcaact aattttttca tcttttcttt
    20161 cctctttctt ttctttttat ccttttacgc cgccttcgac taaatcacct ttaataaatc
    20221 gttcagataa cgcatacata atgatgactg gtaaagcggt caccaatgaa gcggccatca
    20281 ttcgtcccca aatgtagtct ggtgtactga ataatgtgtt caaaccgatt ggtaaagtaa
    20341 atttttcgga ggtcgacagg aaaatcgaag caaataaata atcattccac gaaaccataa
    20401 agcaataaac gaagacagaa atgattccag aaatagccaa tggtataatg atccgaataa
    20461 tgatctggat ccggctaaga ccatcgatca ttgctgcttc ttcaatatca gtcggaatcg
    20521 tgtcaaaata actttttagc atgaatatcg cagtaggaag tgtttgaacg accatcgtga
    20581 taatcagtgc tgtattggta tcatacaaac ctagaccgga aatgatttta aataatggaa
    20641 caacgatcaa aataccggaa aacatataaa cggtgtaaaa gctagcattg attttcgagc
    20701 gagctttgaa gcgtagtttt gacaaagcgt atgccccgct gatcccaatc actactgcag
    20761 ttacggaggc tatcaaggag atcactaaac tatttttgaa atacatgatg aatggaaaaa
    20821 tctcagcatt gaaaatatcc tgataatgtt caaaagtcca ttctttcgga aaaatagttt
    20881 gtgtcgttgc aattgcttct tgtgaatcct tgaatgatgt catcagcatg atcaaaaatg
    20941 gaaacaaatt gacagctaat agcaacaaaa tcgtcgcgta aaataagatt ttcctgctct
    21001 ttttattttt catttcaagt ccaccttctt tcgtgagagc aagacgaccg ctgcaataat
    21061 gatgaacaat agaatcgata tagctgctgc ttttccaaga tcattgaacg caaaggctgt
    21121 ctgatacaaa taaacgccta agatattgac tttattcgtc agtagataga catctgtaaa
    21181 catatagaac atccagatag atcgtaaaac aatcacagtt gctagtactg gtttgattgc
    21241 cggcagtgtc acgatcttga acttctgcca agcatttgca ccgtccattt cagctgcttc
    21301 ataaagcgtt ttgtcgactg tttgcaaaat agccaaaaat gaaataaacg catacggata
    21361 gtatctccag atagcaaaca aaacgaccat aaagaagcta gcaactggct ggtcgaacca
    21421 cagcggtgct tcgttgaaaa gctgcaacct atctactaac agatcattga cgattccata
    21481 actattattg aacatatatt tccaagcaaa aatcaaagaa atagatggcg taacataact
    21541 taataaaatc agtgaacgca ccgttttcct aaatctgaac ttgcggttaa gcaaaatagc
    21601 tacggctaat cctaaagctg tactgcctgc tacaactaag aatgtgtaag caactgttaa
    21661 acctaaagaa cgataaaatt cactatcact taaaatttca agataattgc ttaatccgat
    21721 aaaaacggcg tctgagttag gattcagcgg catatcgaaa aaactgatct gaatattgga
    21781 aagcattgga atcagcacta aaacagctaa taaaagcatg cttggtgcta aaagtcccat
    21841 ggctaatttt aagtcagaat gtttccttgg acgattcatt gaaacctccc cctgataatc
    21901 tgccaatagc tatctacttt tttcgaacac cattggcaga acttcttgtt atttcgtatt
    21961 tttttgtgcg tcagctaaag cttgatcgac atcttcgttt ccgacagtta cttggttcac
    22021 tgcttttccg atcacacctg agcttgtgat atctcccatt ttcgtgaaat tcttttcccc
    22081 gactagtcca aagacttgta cttcatcaaa agcactagca atctcagatg gcagctcgcc
    22141 aaaagacttg atgacttcat tttctttgta accatccagt tcgactactt gtttattcac
    22201 tggttgagca ccaccaggag acatcaagac ccacttttcc atgtttttcg gttgagataa
    22261 gaaagctgtg aatgcttcag cagcctcttt ttgtttctcg tctagaccag aagaaatcgt
    22321 caatccagat actgtgccat agacagcttg atctttttgt gttgggatag caaaaccgat
    22381 attttctgag ttccccgctt cataaacaga aggcaaaatg taagttgaat agattgccat
    22441 tggtgctgtt ccattcatga aagcatcttt gatttctgtc acatcattag agcctggcat
    22501 cgtgtattgg gataagtttt gataaaacga caatgcttct ctcatctttt ccgtatcgat
    22561 cgttacctcg ccttcatcat ccaaaacatt tgctccattt gacaaagcaa attgagagaa
    22621 cgcttgctca ctcattgttc cttcagcagt aggcatagca attccatatt gtttattctc
    22681 tttatctgtg aaatgttgag caatttttaa aatatcatcc caattttctg gttctgaaaa
    22741 accagcttct gataattttt ctttgtcata ccaaatacct tgaacccagc cgctaattgg
    22801 tgcagcaata tagctttttc catcttctga acgaacaagg tttttggctc cgtcataata
    22861 attatcttct ccagcttcct cgataacgtt ttggactgcc gtctgatcaa tcagttcatc
    22921 tttatccatc actttagcaa aatcctgact gacttccatt acttctggta atttaccgga
    22981 acgagctaac gtaacgattt ttgtattgaa cgctgattct tctaccggca cttgcttgat
    23041 tttgatgtct ggattttctt tttcaaaatc agcaaccagt tcattgatca cacttaatcg
    23101 ttcctgttcg acagaagagt gcatgaattg aatcgttgtc tgttgtgatt cgttagagct
    23161 ttgactttca ccacaagcag taagaagaaa tccagtagct agcagacagg tagtaacaat
    23221 aatttttttc atttttttcc acctcttatt ttattatttt cagccacata tattcatacg
    23281 gctggaatat caactttcgt tcttgactgc aatggataat ttgtccagaa aacaggtcta
    23341 ggtaatctcc ttttggtaaa agataacctg ctgattgatt ggaaaaatta tggataacta
    23401 agactttctc ttccttcgct tcgcgaataa aactgataaa agaatctgct gagtccagta
    23461 agtaaatcaa actattcgga tggaaggctt tttctttttt tcgaatagat agcatctggg
    23521 tcaattcacc atatactgct gttcttaatg gatctgtctg caactctttc tcaatatctg
    23581 ctaacaaaaa tttttgccga ttgatttttc gattctctcc tgttttttct acgcctttgt
    23641 aatcatttcg acttcccagc acactttgaa tataaattgc cggcacgcct ggtaacgtta
    23701 ccaaaaatga atgagcagct aaaaagcgtt tgcttctcca ttccgtcgta tctgttggag
    23761 aacttaacgc atctagatac gtagcgttta tctcatatgg actggtcgtt ccatctgggt
    23821 ttttcttgta gtttactcgg gcaccctctt tcttcagctg ttccactaat tcaaggatcg
    23881 ttttttccgg cagaatacct cgtaccggat tcaatccgat accgtcatgg gaggctaaaa
    23941 aattaaaaaa agttgttttt ttcgatgcga aatcgatggt ttttaaccat tctttcaact
    24001 tacttgtatt acccgaacga atcgcttgaa gggtcaaagg tggtagcgaa aactgataga
    24061 ccatctgtgc ttcattctcg ccattcccaa aataactgat attatctttg tgtggcacat
    24121 ttgtctcagt gatcaatacc gttcctggag ctgcttcatc agcgattgca cggaataatt
    24181 ggatgatttt atgcgtttct ggcaggtgta tacaagaagt tcctagttct ttccataaaa
    24241 atcccacagc gtccaagcga ataaatgaag cgcctttttc taagtaaaac aaaagaatat
    24301 cgatcatttt caataaaact tctggatttt taaaattcaa gtctacttga tcttcgctaa
    24361 aagtcgtcca tacgtgtttt tctttcccgt ttttgtcaat gaacgttgta agtaacgggc
    24421 tggtccttgg tctagtcacg ctgcttgtat ctgtatccag tggcaaatca ataaaaaaat
    24481 ctttgtacgt atcattcaag tttaaatatt cctgaaacca agtactttcg gcagacatat
    24541 gattgcagac aaagtcgaac attaaccgtg tttcgcttct catttcttct atatcttccc
    24601 atgtgcccaa acggtcgttg attgcctgat aatttacaac ggagaaacca tcatcagatg
    24661 tatatgggaa aaatggcaag aaatgtacaa ctctaaatgt atctgccaaa tacttttgat
    24721 aaaatttctt aaatgtacgc aaagttggtt gctgctcttc aaaaaattga tccccataag
    24781 taatcaagaa aatatcttct tcgtcccaat gcagtttgcc tgcctggcct ttcttctgct
    24841 gataaacact gactttttgt tcaatcatag aggcggtttt ctcagacaaa tctgagtcgc
    24901 gataaatcgc ttgtaaaagt tttgtataca tcggattccc ctttcttttt tgggagcgtt
    24961 cccaacgatt tgatcatatt atggaaaacg cttactgtca atagcatttt tttaaataat
    25021 tttgatatac acaaaaatac aaataagaaa gcttatgtat caataattaa tagcttctta
    25081 aaaaaatatg gtctctcatg atcatgcaag ctattcctaa attttcaaaa tcatagacaa
    25141 aaaagaatgt ctattgtact atggaattat tctaaaacgc tcccaaaaag gaggtgcata
    25201 taaaattgaa acctactatt tatgacattg cgaaaaaagc taatgtctca aaatcaactg
    25261 tctccagagt attaaataac cagccaaata tctctgataa agcaaaagca agtgtacttc
    25321 aagcaatcaa agaattgaat tatgaaccga gcaaaattgc aagaggactg tcaggaaatg
    25381 gttttgacgc gattctagca ttatctcatc gtacttctca tacgacctat ggcaatcctt
    25441 ttttttcaga tgttattcaa tccatttctg ccgtttctga acaaaataac ttcgacttga
    25501 ttctacaaac ggcaaaaaca ccagaagaag agctagaaaa atgcgcgaaa aagatccaag
    25561 aaaaaatgat ccgaggaatt attattttaa gctctacgat ggatgaaaca cttctttttg
    25621 aattggatca atataatatt cctattgtgg taatcggaaa agtcggaaac gactacacga
    25681 acatctactc agtggacacg gataatttcc aggatagtta cgatttggtg gaaacactga
    25741 tcaagaatgg acaccgtcat atcggctgtc tccatgctcc gacagaagtt tatgtatcca
    25801 atgatcgtct caatggttac cgtcaatgct tatttgatca ccacctagat atccgtaatg
    25861 aatggattat agacggcggc tttcgcttag aagatagcat ggcagcaatg aatgatttaa
    25921 tgaagctacg agaaagacca actgcgattt tttctaccga tgcattaaaa tcattgagta
    25981 tttacaaagt ggcagaacaa tctggaaagc agatacctga tgatctttcg atcgcaggat
    26041 tcagtgatcc cttattgtcc tccctatttt cgccacagtt gactcggata gacattccga
    26101 caaatcagtt aggaatcaaa gccacagaac ttttattcca actaatccac caccaatacc
    26161 ctgaagaaaa aagaatcatt ctttcaaccg agattactct cacagattcg atccaacaac
    26221 aagaaatgta gaacagaatt ataaaagaac agaaacactg aggataagtc agttactctg
    26281 ttcttttttc aatcactcat ccctcgttac cccctcccgg tattttagcg aatttttctt
    26341 attttccatc tgttttagat agctgtggat aactataatg tttgtgttaa actatttttt
    26401 ccacagggaa gaaaaaacta tttttaacca cgttgtgtat aagtttttat agttcgtttt
    26461 tttaaacaca tttaccctta tttcaatcca tgttatttgg ggatacttat ctctaaaaca
    26521 taaatagaat aacagacatg tctactggtt ttgttttatt cgaggaatta ttcaataata
    26581 gctagcaaaa tattaaaaat aaccgctcta tcttcccatc aatttatttt gcattcaaaa
    26641 aatgatctat ctttactttt gttgattgac ttctcttgga agatgatcgt tccgatacct
    26701 taattggata cgaaccgatg ttgttaagat cctttcccac ttttcttcta gttagacaaa
    26761 ttattttaga caaaccaaaa aagaggttgt gacaataatt tgtcacaacc tcttttttaa
    26821 aatatctatt ttgtttgatt gttcattgcg tcttctgcag ccatttgcgc ttcaatatct
    26881 gaaatactgt agacattttc gcttgctaca ccgacttctt ttggttccat gtcacgataa
    26941 cgttgcatac cagtaccagc tgggatgatc ttacctataa taacattttc tttcaaacca
    27001 agaagaggat ctttcttacc gcggatcgca gcatccgtca agacacgagt tgtttcttgg
    27061 aatgatgcag cagataagaa actatttgtt tctaatgatg cttttgtgat acctagtaag
    27121 acaggtcgag aagttgcagg aacgcctcct gagactagcg tattgtagtt ttgatcttta
    27181 aagtctgcga tgtctaataa tgtacctggt aagatatctg tatcacctgg atccattaca
    27241 cgaactttac gcagcatttg acgaaccatt acttcgatat gcttgtcgcc gatttctacc
    27301 ccttgcatac ggtaaacacg ttgtacttca cgaagcaagt agttttcaac agataataca
    27361 tcgcgaactt gcaacaattg ttttggatcg attgatcctt ctgtcaatgg tgcaccacgg
    27421 tggatcatat cgccttctgc tactttcata cgtgctgtat aaggaacagt atacgtacgt
    27481 gtatctgttt ttcctttgat tgttacttct ttggtacgtg ttgcaggatc ttctgagata
    27541 tcgatgacat cccctgttac ttctgtgatc actgcttgcc ctttcggatt acgtgcttca
    27601 aagatttctt ggacacgagg aagaccttga gtgatatcgt ctccggcaac cccacctgta
    27661 tggaatgtac gcatcgtcaa ctgagtacca ggttcaccga ttgattgagc ggcgatcgta
    27721 ccaactgctt ctccgacttc aacgtcagag ccagttgcaa ggttgcgtcc gtaacaatgt
    27781 ttgcagacac catgttttgt attacatgtg aagacagaac ggattgtcac tgtttcgaca
    27841 cctgcatcaa cgatttgttt tgcgatgtct tcggaaatca attggtttcc tgcaatgatc
    27901 aattcatttg tttcaggatg acgtacttct ttacgggtgt aacgtcccac taaacgatct
    27961 tctaatgatt cgatgatttc atttccctcg cggatggctt ggatttcaag acctcggtca
    28021 gtaccgcaat cttcttcacg gatgattaca tcttgggcca cgtcaaccag acgacgagtc
    28081 aagtaacctg aatcggcagt cttcaaggct gtatcggtca tccctttacg agccccgtga
    28141 gttgagataa acatttccaa gacggatagt ccttcacgga agttcgaaat gattggcaat
    28201 tccatgattc gaccattagg agcggccatc aaaccacgca ttccggcaag ctgtgtaaag
    28261 ttggaaatgt taccacgagc tccagaatca gacatcatga agattggatt tttcgcttcc
    28321 aaaccttcca tcagtttttg ttggatgctg tcttttgcag cattccaaac agcaatgaca
    28381 cgttcataac gttcgtcatc tgtgatcaga ccacgacgga attgttttgt aatagtttca
    28441 acttgtttat gggcattttc aatgatttct tgtttttcat tcagtacact gatgtcagcg
    28501 atccctactg tgataccggc atgtgtcgaa tgtttgtacc ctaagtcttt catacggtca
    28561 agcatcttag aagtttctgt tactttgaat cgtttgaaga cttctgcgat gatgtttcct
    28621 aagtttttct tcttgaacgg taatactaat tcttgctctt tgatatgtgc aggaatatca
    28681 gtaccagctt caacaaagta tttgtccggt gtttggacgg tcaagttgaa gtctgttggt
    28741 tcattcagat aagggaattc aggaggcatg atctcgttga agatgatctt accaactgtt
    28801 gtgatcatcg tacgttcttt ttgccattca gtaaatggtt tttcacctaa tgagttaggg
    28861 ttcacaccga tacgtgaatg taaatgaaca tatccgttgc gccatgcgat aacggcttcg
    28921 ttttgatctc tgaagaccat tccttcgcct tcgcgtcctt cttcttccat tgtcaagtag
    28981 tagttaccta gaaccatatc ttgagaagga gtaacaactg gtttaccatc tttagggttc
    29041 aagatgtttt gagcagctaa catcaacata cgtgcttctg cttgtgcttc ttcattcaat
    29101 ggaacgtgga cagccatttg gtctccatcg aaatcggcat tataagcttc acatacaagt
    29161 ggatgcaaac ggattgcgcg tccttgtact aaaactggtt cgaatgcttg gataccaagt
    29221 cggtgaagcg taggtgcccg gttcagcaat actgggtgtt ccttgattac gtcttctaaa
    29281 acatcccata cttcgtcttc ttgacgttcg attttacgtt tcgcattttt gatgtttgaa
    29341 gctagttcgc gttgaacaag ctcacgcatc acgaatggtt tgaataattc aatggccatt
    29401 tcttttggaa gtccacattg gtacatcttc aagaaaggtc caacaacgat aacggaacga
    29461 cctgagtagt cgacacgttt acctagcaag ttttgacgga aacgaccttg tttccctttc
    29521 aacatatgag aaagagattt caatgggcgg ttacctggtc cagtaactgg acgtccgcga
    29581 cgaccgttat cgatcaatgc atctactgct tcttgcagca tacgtttttc attttgaacg
    29641 atgattccag gtgcattcaa gtctaacaaa cgtttcaaac ggttattacg gttgattaca
    29701 cgacggtaaa gatcattcaa gtcacttgtt gcaaaacgtc caccttctaa ttgaaccatt
    29761 gggcgtaaat ctggtggaat aacagggata acgtccataa ccatccagct tggttgattt
    29821 cctgaagcgc ggaatgcttc taaaatatcc aaacggcgga ttgcacgtgt acgtttttgt
    29881 ccttgtgcag atttcaattc ttctttcaat tcggctgctt ctgcatctag atcaacatta
    29941 tcaagtaatt gtttaatcgc ttctgcaccc atagcagctt ggaattcttg tccatattgt
    30001 tcacgttttt cgcgatattc gcgttctgtc aacaattgtt ttttctctaa attcgtattt
    30061 cctggttcga tgactacata tgaagcaaaa taaatgattt cttccaaagc gcgtgggctc
    30121 atgtctaata caagacccat acgtgaagga atccctttaa agtaccagat atgtgaaaca
    30181 ggagctgcta attcaatatg tcccatgcgt tcacggcgaa ctttcgaacg agtaacttcc
    30241 actccgcatc gatcacaaac gattccttta taacggatac gtttgtattt tccgcaggca
    30301 cattcccagt cttttgtagg accaaaaatc cgctcacaga acaacccttc acgttcaggt
    30361 tttaaggtac gatagttaat cgtctcaggt ttttttactt caccgtaaga ccagcttctg
    30421 attttttcgg gagaagccaa acctatttgc atactttcga atttatttac atcgatcaaa
    30481 aggggttccc tccatttcat ttaatggcaa accgcaagac ggctgatctt cgaccgcctt
    30541 gcagctcata gaccggatta gtctaaatta tcttctgcgg tttcaaagtt ttggatgaca
    30601 tcgtcttttt catcttccac ttgttcagca gctttctttt ctaattcttt ggcagtttgc
    30661 tgttcggcaa attttgtcaa agcatcaaca gtgatcaaat cgtcgtcttc atcgtccatg
    30721 tcgcgaagtt cgatctctgt ttcttcgata tccaagacac gcatgtccag acctagtgat
    30781 tgcaattctt tcaccaatac gcggaatgat tccggtacac ctggttttgg aattggttca
    30841 cccttgacga ttgcttcgta tgttttcaca cgtccgacta cgtcatctga tttgtatgtc
    30901 aagatttctt gtaaagtata agccgcaccg taagcttcca gtgcccatac ttccatttca
    30961 ccaaaacgtt gtccaccaaa ttgagcttta cctcccaatg gttgttgtgt aaccaatgag
    31021 taaggtccga ttgaacgtgc atgcaatttg tcatcgacca tgtgagcaag tttgatcatg
    31081 tacatcacac cgacagacac acgtccatca aatggttcac ctgtacgtcc gtcgtacaga
    31141 acagttttcg catcgctggc cataccggct tcacgaactg tttcccatac gtcttcatca
    31201 cttgcaccat caaatactgg cgttgctacg tggataccta actgacgtgc agccataccc
    31261 aagtgtaatt ccaatacttg cccgatgttc atacgtgaag gcacccctaa aggattcagc
    31321 atgatatcga ttggtgttcc atcaggtagg aatggcatat cttcttccgg cataatacgg
    31381 gatacaaccc ctttatttcc gtgacgaccg gccattttat ccccttcatg gattttacgt
    31441 ttttgaacga tataaacacg aactaacatg tttacgcctg gtgacaattc atctccagct
    31501 tcacgagtaa agattttcac atcgtgaacg ataccgccgc cgccatgtgg tacacgtaat
    31561 gatgtatcgc ggacttcacg agctttttcg ccaaagatcg catgcaatag acgttcttct
    31621 gcagataatt ctgttacccc ttttggcgtg actttcccta caagcaaatc gccatcttgg
    31681 acttctgcac caatacggat gatacccatt tcgtctaaat cttttaatgc gtcttccccg
    31741 acattaggga tttcgcgtgt gatttcttca ggtcctaatt ttgtatcgcg ggcttctgat
    31801 tcatattctt caatatggat agaagtgtat acgtcatctt ttaccaaacg acggctcatg
    31861 atgatcgcat cttcatagtt gtacccttcc caagtcatga aaccaactaa aacgttttgg
    31921 cctagagcca tttcgccttg ttccatagat ggtccgtctg ccagagtatc ccctttttcg
    31981 actttctcac ccaaatgaac gattggacgt tggttgtagc ttgttccgga gtttgaacga
    32041 cggaattttg ttacatcgta tttatctaat gcaccgttgt cgcggcgtac gcggatttca
    32101 gaagcatcaa cgtattcaac gacaccgtca tgtttacata acaaagcagc tcctgagtca
    32161 tgcgctgatt tgtattccat accagtacca acccatggtg cttgaggatt gatcaacggc
    32221 actgcttgac gctgcatgtt ggcacccatc aaggcacggt tggagtcatc gttttccaag
    32281 aaaggaatac atgctgtcgc aacggcaact acttgtttag gtgatacgtc catgtagtct
    32341 actttatcaa tagatacttc taagttttca ctttgagcac gagccataac aacgtcttga
    32401 gcaaatgttc catcttcatt caatgggctg tttgcttggg ctacgatgta atggtcttcg
    32461 atatcagctg tcaagtagtc gatttggtca gtaacacgac ctgtttcacg gtctacacga
    32521 cgataaggtg tttcgataaa tccgaattta tttactttcg cataactgga caaactattg
    32581 atcaacccga tgtttgggcc ttcaggagtt tcgatcggac acatacggcc atagtgggag
    32641 tagtgaacgt cacgaacttc ataaccggcg cggtcacgag tcaatccgcc aggtcctaag
    32701 gctgaaagac gacgtttgtg tgtcaattcg ccaagagggt ttgtttgatc catgaactgt
    32761 gacaactgag aagaaccaaa gaattctttg atacttgcca caactggacg gatattgatc
    32821 aattgttgag gtgtcaatgt ttcagtatct tggatcgaca ttctttcacg aaccacacgt
    32881 tccatacgtg ctagcccgat acggaattgg ttttgtaaaa gttcaccgac tgaacggata
    32941 cgacgatttc ctaagtggtc gatatcatcg acattgccga taccttccat caagttcaag
    33001 aaatagctca ttgaagcaat gatgtcggct ggacgaaccg tttttacagc tgcttctgga
    33061 tagccgtttc cgatcacgtt tacttcacgt tcagggtctc ttggtgaaaa tactttgatg
    33121 acttggacag tcattggatc tgtgaccaca ccgtcttctg atggatagta agtcactgaa
    33181 tttaggccat tatcaatgaa aggagctagt gtttccatga cttggtgagt cagaactgtt
    33241 cctttttcaa cgatgatctc gcctgtttct ggatcaacta atgtttcagc taatgtcaga
    33301 ttcaatagac gtgttttcaa atctaatttt ttgtttactt tgtaacgacc aacgtttgct
    33361 aagtcgtaac gttttggatc gaagaaacga gcattcagca agttacgtga gctgtctgct
    33421 gtttttggtt cgcctggacg caaacgttcg taaacgtctt tcaaaccttc ttctgttctt
    33481 gagtcgcttg cgtttttatg caagtctttt tcgactgtat tgcgtaatgt ttcgctgtcg
    33541 ccgaagattt caaagatcgt atcatcggaa ccaaatccta acgcacgaac taatacggtt
    33601 aaaggaatct tacgtgtacg gtcgatacgt acataagaga tgtcttttgc atcagtttcc
    33661 atttctagcc aagctccacg gttagggata acagtagaac cgaatccttc ttttccgttt
    33721 ttgtcgactt ttccatggaa atagacacct ggtgaacgga ctaactgaga aacgattact
    33781 cgctctgccc cgttgatgat gaatgttcct tgttctgtca ttaatgggaa atcaccgaag
    33841 aatacttctt gtgctttgat ttcgccagtc tcgcggtttg tcaaacgcaa tgttacatgt
    33901 aatggtgcag agtagtttgc atcatgcgca cgagcttctg ctactgtata ctttggttct
    33961 ttcaattcat aatctacaaa ttccaaagat agattgccat tgaagtcatc gattggcaaa
    34021 atgtcttcaa acatttctct aagtccctca tctaagaacc attggtaaga gtctgtttga
    34081 atttcgatca aatttggtaa ttccaacact tcactgattc ttgcgaaact tctacgttcg
    34141 cgatgttttc cgtattttac tacgtgtcca gccaagctct tcacccctct aaaatgtcat
    34201 tcaaaaagac tacccgatca attgttacaa taatatttca aatattattt ttaggtaaca
    34261 acagacattt tttgcggctt ttaggcaaaa aaaaaccaaa aatagaacat tctcttgaaa
    34321 ccttacttcg tgagcttcta cttttgtttc cttatattaa agccgaaacg gacaatattt
    34381 cgtttctgcc ttttcttcat cagatagttt ttgcaacttc atatactaca cttattttct
    34441 ataaaagtca acacttcctg gcgtttcttc tgctttttcg caacattttc ttaatgtttt
    34501 caattttatg ttttcaatta tctgacaatt cttaattgac agaccttttt atctcggtta
    34561 tactttagaa aattcatttt gtggaggaac ttctatgcga accaacatca accaaatcgc
    34621 accaacacaa ctgaataact tttactttag ctactttaga taggtttgta ttcatcatga
    34681 tggatacaaa cgttaaacga cagtttgtat ctatgatggc taaagaactt tgtattgcag
    34741 caaaaagcca cgtagacaaa atgctaagtg gctgagcagt gaaaatacaa agtaaaagta
    34801 tcaagcattc tactttttca atttattttc cgacttttca atccatttgg acattttgtc
    34861 tgaatggatt ttttgtttcc caaaattaag gaggaaaaag aaatgaaaaa aaatataatc
    34921 atcggattag gcgctgccct gctactagct ttaggaggtt gcgcacaaaa agacaattcc
    34981 gcagaggcat cagatggaca aaaaacaatt ggcgtattgc aacttgtaca acataactcg
    35041 ttagatgctg cctataaagg attcaaagaa ggattagcag aagaagggta taaagaaggc
    35101 gataatctaa caatcgatta tcaaaatgcc caaaacaatc aagacaacct gaaaagtatg
    35161 agtgagaaac tcgtcaaaga agatcctgat ttgctacttg gaatcgccac tccagcagca
    35221 caatctttat taaatgaaac aaccactatc ccgatgactg tcacagcggt cacagacttg
    35281 gaacaagccc atttagtcac atccaatgaa aaacctggcg gcaatgtgac tggcacgaca
    35341 gatatggtgc caatcgaaaa acaaatcggc ttactgttaa atatcgtgcc agacgccaaa
    35401 acaatcggca tcatgtataa caacggtgaa gcaaactcta agatccaagc tgatttggca
    35461 gaaaaagaat tgaaaaaagc gggcgtcaaa gtcaaagtac tgacagcaaa ttccacaaat
    35521 gatgtccaac aagtgacgaa aagtctagct aaagatgtag atggaatcta tattcctact
    35581 gataacacgt tcgcaagtgc ggcaagtgtt gtcggtgaag tcgcaaaaga aacaaaaaca
    35641 cctgtcgttg ctggcgcagt tgatcaagtc aaagacggcg gtctagcaac agtaggaatc
    35701 gactacgaaa gcttgggcaa acaaacaggg atcatggcag caaaaatctt agacggcaag
    35761 aatccaagtg aacttcctgt cgaaacagcg aatgatttgg aactgtatgt caataaagac
    35821 atggccaaag cattaggcat caatccagac agcatcaaag aagctgaata aaacacagga
    35881 ggattctcat gacaaatttg ctctctcttt tcttatcatc tgcatcgcaa ggggtcttat
    35941 gggccctact tgcgattggt gtatacgtca ctttccgtat cttggacatt gccgacttga
    36001 ctgctgaagg aagctttcct ttaggtgcag ggatcgcggc agtaagtatt acaaatggct
    36061 atcatccatt gacagcttgt cttctaggct ttttaggggg tgcggcagca gggcttgttt
    36121 ccggactgct tcacacaaaa ttgaaaattc ccgcccttct tgcagggatc atcaccatga
    36181 ctggtttata ttctgtcaca tcccgtgtga tgggtgcgcc gaacatctct ttattgggtc
    36241 agccaacgat ctttcattgg gtcgagtcat tcggcataac aaaagacaac gctgtactga
    36301 tcgtcggtct gctttttgct ttgatcgttg tatgtttact tgttttgttc ttacgtacag
    36361 aaacaggact tgctattcgt gcaactggcg ataatctggc aatgagcgaa gcaaacggga
    36421 tcaacacaga taatatgaag atcatcggtt atatgatttc taatggatgc attgctcttt
    36481 ctggctcctt acttgcgcaa aacaacggct ttgctgattt aaattctggt attggaacga
    36541 tcgtaattgg acttgcttct attatcatcg cagaagtttt gttacgtaac cagccactat
    36601 ttctccgtct cttgactgtc gttttaggtg cggtcattta tcgttttatt ttagcgttag
    36661 tcttcgaact gaatgttgaa ccaagtgatt cgaaacttgc ttctgctttc gtgctagtga
    36721 tctgtctttc tttcccgcag atccagcaaa aattccattt acctcaaaaa aagaaaggcg
    36781 gtagtgtcaa atgaatgacg tcttaacatt ggaaaatctc catcatacct ttgaaaaagg
    36841 aacgatcaac gaaaatcatg tcctgaaagg cattaatctt tccttagatc ccggcgactt
    36901 cgtgacgatc atcggtggaa atggggctgg taaatctact ttgctgaaca gcatcgccgg
    36961 tacattttct gtcgatcagg gtcaaatttt attgaatgga aaaaatatca cgaaaaaatc
    37021 tgttgtcgaa cgttcgaaat ccatcagtcg cgtattccaa gatcctaaac tagggacagc
    37081 cgttcgttta acggtagaag aaaatcttgc tcttgctatg aaacgaggaa aaaaacgcgg
    37141 cttcttccgt ggtgtcaaac cacaagaccg aacatttttc aaagaccagc tggttcggct
    37201 aaatttgggg ttggaaaatc gtttatcctc agaaatcggt ttgctgtctg gaggacaacg
    37261 tcaagctatt acgttattga tggccacatt acagcgtcca gaactgatct tattagatga
    37321 acacactgca gcgcttgacc ctgctacttc caaaacggtg atggaattga cagaaaagct
    37381 gatcactgag cagaatctca ctgcttttat ggtcactcat gacatggaag acgcgatccg
    37441 ctatgggagt cgactgatca tgctgcatca aggaaggatc gtcgtagata tccagcaaga
    37501 ggaaaaacag cagctcacag tgccccaatt attagcgttg ttcaaacaaa acagcggcac
    37561 ggcattaaaa gacgatcaag tacttttatc ttcataatca gaaagaggat gggacaaaaa
    37621 atgaacgccc accctcttct tttttctctt tccttctttc gtttttgctt tatgtatcaa
    37681 ccgttgtttc ttcgaaaact attttgttct ctccttcttc ctgatagaat ggatgggaaa
    37741 ggagcttgat gccatgacaa cgaaagacaa ggtacttcaa ttattgaaaa agacaccaga
    37801 ttttctttcc ggagaaaagt tggcacagca actggaagtt tcccggacaa gtatttggaa
    37861 agccatcaaa gaattggaaa aggcaggcta tcgttttgag cacgggccta cagggtatcg
    37921 ttacctgccc tctgacgtac tagatgcaaa agaaatccag caagagctac ggtcgtttat
    37981 tcctgatttg aaagtacaaa cgatgttgac ttctgaatct acgatgaaag atgccaaact
    38041 ggcagcagct caaggagatt ctgtttcgaa gttatttgtt gctgatacac aagaacaagc
    38101 tcgtggacgt tttggacggc cttttttcgc tgaacctgga cgaggaatct acatgagtct
    38161 cttgctccat ccagacaaac ggtttgatga attgcctcaa tatacgatca tcgcagccag
    38221 tgcctgtgtg aaagccattg aatctgtaac cggtcgttct gcttcgatca aatgggtcaa
    38281 tgatatctac atggatggaa aaaaagtctg tgggatttta tctgaagcag tcagtgatat
    38341 ggaaagcggc aggatcagtc atatcatcat tgggatcggg cttaattttt ctgttcctca
    38401 aaatcatttt ccagctgatc tccaagaaaa agccatctct gtctttcccg acggacaagc
    38461 tagtgtcaca aggaatcagc taatcagcaa aatctggcaa gaattgttca cttatctttc
    38521 tcacccagaa aaagcgttgg acttctataa agaaaaatca ttcgtattgg gaaaaaccgt
    38581 gtcgttcaat caaaaaggaa cgacttatgt cggtcttgca aaagaaatcg gtgatcatgg
    38641 cgaactgatc gtccaaacaa atgaacagaa gatgagtttg tcttctggcg aaatcagtct
    38701 cacatctatc cgtacgcaat aattatcaga tttcacaaaa ggacacctga tctcatacag
    38761 tgtccttttt tctgaattct cttcaaaaga ctctccttat tctatcagct gacagtttct
    38821 ctatcctcta gaaaaaggta ttctaaaaat atctataaag catggtacac tttctttaaa
    38881 tgaatcaatg caaaaaacaa gagagtcatc agtttacacg atcaaaagag atagcaggcc
    38941 gcttcctttt tcaatcttat agatagacca tgggtaacga taaaaacaaa aaagaacttg
    39001 cgacttattt tgccgtaagt tcttttttag ctagtgcctt aatctcttaa tcccaacaaa
    39061 cgtctcgcat agatcacctg accagagtgc atagcagcat catcaataat agaaacgaga
    39121 cgcattcctc gtgtgactgg tggtgtccag ttctcgtcaa ttacttcatc tagctcggaa
    39181 ggttccagcc ttcttaagta agaaatagca aaatcagcag ctcctcgcaa gtatcctagc
    39241 agatcttcta tttgattagc ttggatctgc tttgcttgtt ccagactatg cttccaatct
    39301 tgttcgttat ctgctatcgt catcggaaac cggctttccc agtcttgact atgccaaatt
    39361 ggagaagttc ctgctaaatc agcaatctgg aaatctaatt ctcgagcggt atgccaagcc
    39421 agccaagtca ttgatttgat ctgcggggct ctatcttcca ctggaaaacg attggcctcc
    39481 tcaatagaga cgccttcaaa caaacgttca aaccgatcgt atgcccgatc cacactctcg
    39541 catagcatat ctaaataatc catttatttt cctccttttt atttatccta cttacttttt
    39601 cctaattcta ggatacctac ttatatgtta ctacacaccc ttatttttta cactttaaca
    39661 gtcaccatca tcaatagttt ggagattgaa agaaaaagat agtgtcgaac tatcaattga
    39721 acaagaagca acaatatgaa cgtttcatta atattcttta aataaacaat gatttttcaa
    39781 acgaacagtt taagactagg agaaaaagag tataatagga aaatgtttga aaggaaagga
    39841 aggggaaaaa tgaaaaaaag ggcaagttta ctagtcctag ttgctttgtt gttcaatatc
    39901 ttttcgtatg gtgtggccgt ttcagcggaa acatacattc ctaaagaccc tgggaatatt
    39961 tataatgatc taactggtaa ctatggcttg ttaggaattg ctagtcaatt ccacgtattt
    40021 gctaaaaata agacaactgt gaatgcacat acagacggaa atatagcaac acatgaactt
    40081 gatgcaaaaa ataattttgg tacagagctt catgaaggtt tattgaacca agaaataaat
    40141 tatatacaaa aaactgattc tttcgctaat tcttcaggca tacctacagg tagcacaaga
    40201 atgaataaat ttgtcgttgg cagtaacgtg gatgtagcac ttcaagatgg tcatcctaca
    40261 gtcaatggat ccaggcttga tcacttaact ccagatgatt tttataaaga tcgtgctggg
    40321 catgaatata ttgatttcga gcaggaattt gctaaattga atgtagcagc aaatgatctt
    40381 gcaacgatca ctccagctaa aacatatact gctgcagatt ttccagacat gaacaatcgg
    40441 acaatcgatt taactgattt atcagatacc gggtttttat tggtaaatat cgatgcagaa
    40501 gtgttaacga tgaatacgcc acttcaaatc attaatccaa atgatcaagt agttgtattt
    40561 aatgtcatca acagttcttc tgcattgaac gtacagtcac caatcaaata caatgatcgt
    40621 tcaaatcatg aaacagaaga tttttcggat gcgaatatct cttggaattt tggaaatgag
    40681 atgactgatt tgacaatcag tgctcctttt caaggaacaa tccttgcgcc taatgctact
    40741 atccgcatta ctcaaaacca agacggaacg attataggaa caaatgtgat tttagatgca
    40801 gctaccaatc gttgggatcc taacgaaata tttatcagcg acaatggtac agatacgaca
    40861 gacacaagcg acagtacaga tacatctacc acaagtgata gctcagattc atcaacttca
    40921 agcgacagta cagatactac ctctaccaca agtgatagca cggatacatc agcttcaagc
    40981 gacagtacag atac
//
LOCUS       DARBEC010000017        38904 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.017, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000017 DARBEC010000000
VERSION     DARBEC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38904)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38904)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38904
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.017"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(59..187)
                     /locus_tag="SCL80_000832"
     CDS             complement(59..187)
                     /locus_tag="SCL80_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEG1106720.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCL80_000833"
     CDS             complement(190..1563)
                     /gene="rlmD"
                     /locus_tag="SCL80_000833"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1106721.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(1674..2729)
                     /locus_tag="SCL80_000834"
     CDS             complement(1674..2729)
                     /locus_tag="SCL80_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1106722.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCL80_000835"
     CDS             complement(2739..4169)
                     /gene="gatB"
                     /locus_tag="SCL80_000835"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEG1106723.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCL80_000836"
     CDS             complement(4166..5638)
                     /gene="gatA"
                     /locus_tag="SCL80_000836"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEG1106724.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKTTFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCL80_000837"
     CDS             complement(5638..5943)
                     /gene="gatC"
                     /locus_tag="SCL80_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEG1106725.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCL80_000838"
     CDS             complement(6049..8085)
                     /gene="ligA"
                     /locus_tag="SCL80_000838"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEG1106726.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTIKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCL80_000839"
     CDS             complement(8102..10336)
                     /gene="pcrA"
                     /locus_tag="SCL80_000839"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEG1106727.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLVNPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            10491..12050
                     /locus_tag="SCL80_000840"
     CDS             10491..12050
                     /locus_tag="SCL80_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEG1106728.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQNVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(12161..14092)
                     /locus_tag="SCL80_000841"
     CDS             complement(12161..14092)
                     /locus_tag="SCL80_000841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1106729.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAVAEA"
     gene            complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCL80_000842"
     CDS             complement(14110..15024)
                     /gene="pfkB"
                     /locus_tag="SCL80_000842"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1106730.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(15021..15773)
                     /locus_tag="SCL80_000843"
     CDS             complement(15021..15773)
                     /locus_tag="SCL80_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1106731.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLLNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(15932..16141)
                     /locus_tag="SCL80_000844"
     CDS             complement(15932..16141)
                     /locus_tag="SCL80_000844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106732.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(16152..17480)
                     /locus_tag="SCL80_000845"
     CDS             complement(16152..17480)
                     /locus_tag="SCL80_000845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106733.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     KQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            17616..17771
                     /locus_tag="SCL80_000846"
     CDS             17616..17771
                     /locus_tag="SCL80_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106734.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(17845..18432)
                     /locus_tag="SCL80_000847"
     CDS             complement(17845..18432)
                     /locus_tag="SCL80_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEG1106735.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCL80_000848"
     CDS             complement(18515..19765)
                     /gene="clpX"
                     /locus_tag="SCL80_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEG1106736.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVFNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVELAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(20012..21301)
                     /locus_tag="SCL80_000849"
     CDS             complement(20012..21301)
                     /locus_tag="SCL80_000849"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEG1106737.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(21455..22387)
                     /locus_tag="SCL80_000850"
     CDS             complement(21455..22387)
                     /locus_tag="SCL80_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106738.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLKEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(22544..23203)
                     /locus_tag="SCL80_000851"
     CDS             complement(22544..23203)
                     /locus_tag="SCL80_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1106739.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSLYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAVFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCL80_000852"
     CDS             complement(23206..24231)
                     /gene="sppA"
                     /locus_tag="SCL80_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1106740.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELAPNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAIR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(24244..24534)
                     /locus_tag="SCL80_000853"
     CDS             complement(24244..24534)
                     /locus_tag="SCL80_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106741.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAQAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            24943..26748
                     /gene="glmS"
                     /locus_tag="SCL80_000854"
     CDS             24943..26748
                     /gene="glmS"
                     /locus_tag="SCL80_000854"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEG1106742.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDASDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(26820..28175)
                     /locus_tag="SCL80_000855"
     CDS             complement(26820..28175)
                     /locus_tag="SCL80_000855"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEG1106743.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(28199..29368)
                     /locus_tag="SCL80_000856"
     CDS             complement(28199..29368)
                     /locus_tag="SCL80_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106744.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(29365..30252)
                     /locus_tag="SCL80_000857"
     CDS             complement(29365..30252)
                     /locus_tag="SCL80_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEG1106745.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCL80_000858"
     CDS             complement(30441..34133)
                     /gene="nifJ"
                     /locus_tag="SCL80_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEG1106746.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIFPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPTMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLDNANPN
                     LAKALFEKTVHDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARRAARAERRKKMEQATKEKKEK"
     gene            34332..34853
                     /locus_tag="SCL80_000859"
     CDS             34332..34853
                     /locus_tag="SCL80_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1106747.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLACPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(35552..36109)
                     /locus_tag="SCL80_000860"
     CDS             complement(35552..36109)
                     /locus_tag="SCL80_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106748.1"
                     /translation="MRNLSNRNKILIIIVVIAVFHLGTNAVLSRIILGPKPPRPEITR
                     GEFDFRLEYEVDGERIVIEDTIVALFDGFSADAGSMAWYRTWRLHLASDRRSRNILLD
                     ELEDGRRIYYVPESANYSMGDVQKKREPNPNWYPFNGVTIEYPRNKTPEIGAKFISGL
                     EDLYDRFGIRLISWEHDPPIENRFE"
     gene            complement(36288..36899)
                     /locus_tag="SCL80_000861"
     CDS             complement(36288..36899)
                     /locus_tag="SCL80_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106749.1"
                     /translation="MNSNRGNKMEYNGSMRIVQIIFWITSGIVIIGGVFLMLPSLIFP
                     FFALISPKIPEPEITYGEFPFRIEYELDGQINIIEDTVIAEFNGCEFSAGSMKRERRW
                     RSRLASGREDLPFGDDLGIYFSRGSAQYYMGENVQSMSLKPHIALRNLEEGFKREVDF
                     ILGESGYIRTVLNFGEAQEVLTQYGITLINWEISEPIVNNFGD"
     gene            complement(37012..37269)
                     /locus_tag="SCL80_000862"
     CDS             complement(37012..37269)
                     /locus_tag="SCL80_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106750.1"
                     /translation="MRAVHVNPTIEITMIGHSKGGAEAANSVAINTNAIIFNPATVNL
                     SAYELDSNTYTANMVAYVVEGDALNVVFGGVYTYWRINHVR"
     gene            complement(37866..38144)
                     /locus_tag="SCL80_000863"
     CDS             complement(37866..38144)
                     /locus_tag="SCL80_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106751.1"
                     /translation="MEKHNITGSMYVDDQWFISTDEVKIAAIRRDYLQKIHKIKDPNG
                     KKARLIITLITSNGKKLKLAGSYDNVKKFEHWYLQKDSISLNKQFEKN"
     gene            complement(38178..38429)
                     /locus_tag="SCL80_000864"
     CDS             complement(38178..38429)
                     /locus_tag="SCL80_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106752.1"
                     /translation="MKEKPINKWSLYKNSMFGVVIFGVCAGLFLFILLQTVTLFSSNP
                     TYGWRDSIALGMFAGGYGFLLLFSILTLSPIHGLIQLWK"
BASE COUNT        10628 a         8397 c         6824 g        13055 t
ORIGIN      
        1 taaaaagact aagaaacaat aacaaaagtt aacacttctt agtctttctt tagcacgtct
       61 atttattaaa gattttaggg ccttttctca tgtttttacg atcattctta ggtgtttcca
      121 tttgtttgga tcggcctcca ccttgtttct ttttgatcaa ttctttcatt ttttcttttt
      181 ggtccatttt tattctcctt taacgaatga agcgactaat tcgatatgat gggtttgcgg
      241 gaaaagatct acaggctgaa ctttcttcac ggcataacct gcttctgcaa ataacttagc
      301 atctcttgca aaagttgccg gattacatga gatatagacg attcgttctg gtgacatttc
      361 gattgctgat tcaataaaat gatgatctaa tccttttcgt ggagggtcaa ctacgatcac
      421 agatggtttg atcccttttt cttgccattt tttcataact ttttctgctt ttccgacttc
      481 ataatgaaca ttttcgattt tattatttaa agcattgaac tgtgcatctt caattgcttc
      541 tggaataact tccatcccat acaccttatc cactctgtct gctaaagaca agccaatcgt
      601 acctattcct gaataagcgt cgatcacaat gtctttttta tctaactcgg caaactcgat
      661 ggctgtttgg tacagatact ctgcttgttg agtatttact tgataaaatg actttgctga
      721 gattcgatag gtttttccta atagctgatc ttcgatatag tctctgccat ataaaacttt
      781 ttctttgtct cctaaaatca catttgtttt ttcttcattg atattttgga tgaccgaaac
      841 cacttctggc aattcttcat gaatagcttc agcaatttcc ttggatttaa agaatttttc
      901 tttacgagta actaaaacga ccatcatttc atgggaataa tgaccacgac ggataatgat
      961 gtgacgaagc tgtccttcat ttttttcttc attatatcct cgtacttgga accgttgcaa
     1021 gatatctctg actactttga ttgcttgatc gatgacagga tcttggatat aaaaatcttc
     1081 gatagggact agatcatggc tattcttttt gtaaaaaccg gtggctaact gtccatcgat
     1141 acggcgaaca gggatctgtg ctttattacg gtagccgaat ggttctgcca ttccgaccgt
     1201 tggaagtaca ggaacttctg gcatcttagc aatcttcgcc attacatgct ctacttgttt
     1261 ttgtttgaag acaagctgtt gttcatactt caaatgggct aaaggtgcga tacctatgcg
     1321 aagtaatgca ttgttttcta caggctgacg atcaggtgat tgtgtaataa atgtttcgac
     1381 ttttccatat ccaaacttat tgcctgcttt tactactagt atttctactt cttctccagg
     1441 gagtgcattt tcgataaata atggataacc atcgattttt gctaccccca ttccttcata
     1501 actcaagtcg acgatttcga ctgtatatcg ttcgtttttc tttactattt gctgttctgt
     1561 catattttgc tcctttaagc ctatttttca atcctactga actctactat aaatcagcta
     1621 attacacaac taaaaccggc actgacctta ctacgttcag caccggcttt tgattaattc
     1681 ttttgattag gtgtttcgtc tgctattttt ccgtcaccgt cgatatcttc atccgttaaa
     1741 cgttcgactt cttttacgaa ttctttgctg acaacctcta attcttcttc atctgtacct
     1801 gtgattgctt catcaggaat ctgatcggta ttggcaaaga actcaatgtg ttgatgaaga
     1861 ttttggaaat gcatcggtgc ttccccgccg tattcaccat ctaagttgat cattactggt
     1921 cgattgatat catcttctgt ttcaacgaac aaactgcttg ttttggtata gattattctt
     1981 ggatcatcga catgtcgacc gccattcagc atgagtgctg caatatgcaa gatctcaaag
     2041 atatttgctg tcttcacaat gattaaagaa aacttcccat catccaattt ggcatctggc
     2101 gcgatctgtt cgaatcctcc aacagagttc gtcagcccta ggaaaaacat cgaagcattt
     2161 ccgatatatt ccccttcatc atacttcaag cgcattttga ttggctttac tcgagggagc
     2221 atctctgctc ctttcgcaag ataagcaaga taaccaaaaa tactctttag ttctgatgga
     2281 acttcataag tcaattctgt caaatagcca cctgcagcga tattgatgaa gtaactgtta
     2341 tcagaaaggc cgatgtccat ttttacggtt tggtgttttt ttatcacttc tgctgctgct
     2401 ttaatattat ctctaggaat ttttaatgct cgagcatagt cgttcgttgt accagctggg
     2461 atgatggcca ttttaggacg gtgttcaagc ggagcgattc cattaaccac ttcgttgatt
     2521 gtgccatcgc caccagctgc cactactaga tcaaaaccta gttctgcgac tctacatgct
     2581 tcattcttgg ctgaatctgg ttctggtgta gttgcatagg cgctggcttc aaatcctgct
     2641 tcttctaatg cctgtaggat atccgccaaa ttttttttca ataattcttt tcccgaggtg
     2701 gggttataaa ttacgcgtgc ttttttcatt ttcagcctct atcgtttcgc taattcatct
     2761 ttaagtagct tattcacgac accaggattt gcttgtcctt tagttgcttt catgatttga
     2821 ccgactagga agccaaccgc acgatctttt ccatttttga aatcatcgac agattgttgg
     2881 ttgttatcca aaatttcatt aatgattggc agtagtttgt tcggatcaga taattgcacc
     2941 caaccatttt tttcaactac ttcttgggct tctccgccat ttgtgatcaa ttcacggaag
     3001 acttttttgg cgatttttga gctgatcgtt ccatcggcga tcaaacgaat cattcctgct
     3061 aagttctcag gtgtcaattt tgtctcagct aattccagtt tctcactgtt caagtaagca
     3121 gaaacttcac ccatcaacca gttagatgct tgcttggcat ctgcaccatt ttccaaaaca
     3181 gcttcaaaaa aatcagacat ttcttttgta agtgtcaata ccattgcgtc atactctgga
     3241 agtccaaact cacggatata gcgttcgcga cgagaagctg gcatctcagg caaactagct
     3301 tttacttttt gaatccattc gtcatcgatc acgaatttcg ggatatcagg ctctgggaag
     3361 taacgataat cactagaccc ttctttgaca cgcatcaaaa ctgttttatt cgttgtttcg
     3421 tcaaaacggc gtgtttcttg ttggatttct cctcctgaaa gaagaacttt tgcttgacgt
     3481 ttttcttcaa aagctaaacc ttttttcacg aaagataaag agttcaagtt tttcaattct
     3541 gctttcgtac cgaactcttc ttgtccataa gggcgaagag aaatattggc atcacagcgc
     3601 atagagcctt cttccatttt cacatcgctg acttctgtaa attggataat tgatcgcaat
     3661 gcttctagat aagcgtatgc ttcttctggt gaacgcatat cagcttcaga aacgatttcg
     3721 atcaaaggcg tgccttgtcg gttcaaatca acgtaagaat aaccgccaat accatgcatg
     3781 ttttttccag catcttcttc taaatggaca cgttcgatcc ggattttttt ctttttacct
     3841 tctacttcga tttcgatcca tccgtcatga ccgattggtt gatcaaattg ggaaatttga
     3901 tatgcttttg gattatctgg gtaaaagtaa tttttccggt caaaatgagt atctttagag
     3961 atttcacagt ttaatgccaa agctgctttc atcccgaatt ccagcgcagc tttattcatt
     4021 actggcagca cgcctggata accccagtcg atcacgtttg tattgctgtt tggttctgca
     4081 ccaaaatgag ctggcgctgg agagaaaatt tttgagttgg tttttaactc gacgtggact
     4141 tctagtccaa tgactgtttc aaaattcatt cgtctttccc tcctaaaata gctggttgtt
     4201 gtttatggaa ttctgttgct tgttcaaacg catatgccgc ttgatacatc gttttttcat
     4261 caaaataatt accgatgatc tgcaatccaa ctggtaatcc ttctgataaa cctgccggta
     4321 cagacatacc tggaagtcca gccaagttga cagggatcgt taagatgtca ctcatgtaca
     4381 ttgtgattgg atcgttaatg ttctcaccga ttccgaatgc aactgttggt gaagatggcc
     4441 cgatgatcaa gtcataatct gcaaagactt tttcaaaatc tcgtttgatc aatgtacgga
     4501 cttgtcctgc ttttttgaag taagcgtcat aataacccgc gcttaaggag aatgtaccta
     4561 acatgatacg gcgttttact tcttcaccga atccttctga acgtgagttg acatatacat
     4621 catctaatga tttcacattt tcagagcgat aaccgtagcg gataccatcg aatcgttgca
     4681 aattggaact tgcttctgat gaggcaatga tataataaac agccacaccg tattttgaat
     4741 gaggcaagct gacttcttcc acaacagcac ctaatgaacg gaatgtttct gctgctttca
     4801 atacagcttc ttttacgcca ggggccacac cttcgcctaa gtattcttta ggtaaagcaa
     4861 tcttcattcc tttgatgtcg cctgtcagtc cctctgcaaa atcaggaact gaaacgcctg
     4921 aagaagtacc gtctttttcg tcataaccgc tgatagctgt caatgctaat gcattatctt
     4981 tcaccgtacg ggtcattggt ccgatttggt caagagatga agcaaaggca atcaaaccaa
     5041 aacgtgagac acggccataa gtaggtttca tccctacgat gccgttaaaa gctgctggtt
     5101 ggcggatact tcccccagta tcactaccta aagaaactgg aatttgtcca gctgcaactg
     5161 ctgctgcaga accaccagaa gagccaccag gtacttttgt tgggtcccaa gcattttttg
     5221 ttttcttgaa ataagaagtt tctgtacttc cacccatagc aaattcatcc atgtttagct
     5281 taccaacagg gatcatatct gcttgataga ttttatccat gaccgttgca tcataaattg
     5341 gttcaaaatt gtaaaggatc ttagatgcag cggttgtcaa gatatctttt gttacgatat
     5401 tatccttgat accaattgga ataccagcaa ggacgttgct ttcatcaatc cctttttcat
     5461 ctaaagcttt tgcctgttcc aatgcttttt cttcgttcaa tgtaatgaat gcctcgacct
     5521 caggctctgt ttctttgatt cgttcaaatg ttgtttttgt caaatcttgt acagtgattt
     5581 ctttcgatac aagcatatga tgcagttctt ctagagaacg attgtgtaat tctgtcatta
     5641 tgcgccagcc tccccattat cgatgattgc gggtactttg ataaagccgt cttctgtttc
     5701 cggcacattt ttcaataatt catcgcggtt ccatccagct tgcgcccgat cttgtcgcat
     5761 cacgtttact gtttcaacca cattcgacgt aaatggaaca ccttccgtat ctacttcttc
     5821 cagcatttca accatatcga tgattttacc taattggtcg gtaaaacttg caagttcttc
     5881 atctgcgaac gcaagttttg ctaatttggc aacgtgtttc acttgttctt cactaatcgc
     5941 catactaccc cttctttcta aacgagttaa aaactttgct ctctatttta acacaagagt
     6001 gccgtgtctg aaattgttta ttgaaatttg tctgaaaaaa ctcatttttc attcttctac
     6061 tgagcgactt ttagccaatg catctgccat ttgatcttct gtccaaactt cgattcctaa
     6121 ttcttgtgct ttggtcagct tgctgccagc atcttctcct gctaccacga tatccgtttt
     6181 tttcgatacg ctaccagtca ctttcccgcc aagattttcg atcgtctctt ttgcttcttc
     6241 acgagtaaat cgtgttagtt taccagtaag gactactgtt ttttctttaa aaggagactc
     6301 gacttcttgc aattgggcat ttcgaagccc tttgtattcg aaattgacgc ctgcttgttt
     6361 taattcattc atcaattcat ggacttcttc gttactgaaa taagtgacta cactgtcggc
     6421 aatgatatca ccgatcgtat caagcgcaat aatcgactca tagtcacttt tactcaatgt
     6481 ttctagatca ccgaaatgtt ctgctaaaat ttttgctgct ttcgctccga catgtcggat
     6541 tcctaacccg aaaatcaacc gctcgaccga attatctttg ctgttgtcga ttgctgtcaa
     6601 aatattattt gctgattttt cttttatttt gtccaacgtc aataactctt cttctgtcaa
     6661 tttgtaaaga tcagcaacat ctgctactag ttttttatca tacatctgtt ctagtacgcg
     6721 cggccccaac ccatcgatat tcatcgcatt tcttgaaaca aagtggttca aaccttcttt
     6781 catttgagca gggcatttag gattgatgca ccgtaaggct acttcttcat ccaaatggac
     6841 aagttcactt ccacaaactg gacaatgtgt aggcaattgg tattcttcgc tgtctttagg
     6901 tcgtttatct aacacgactt gcgaaacttc tgggatgatg tctcccgctt tataaatcag
     6961 aactgtatct ttcaagcgga tatccttcaa cttaatataa tcaccgttat gcaggctggc
     7021 acggcttact gtcgttcctg ctactcggac tggtgtcatg atagctgttg gcgttactac
     7081 ccctgttcga ccgactgtcc attcgatgtt ttcgattagc gtttcaactt cttctggcgg
     7141 aaacttgtaa gctgctgccc atctcggtgc tttgatagta aaaccaagtt gatcttgtag
     7201 cgagaattca ttcactttga tgacgattcc atcgatttca tagggcaaat ccacacgttt
     7261 gtcatgatat tcttcaatgt atgaccacac ttcatcaatc gaatgacata gacgtttttc
     7321 ccgattcgtg tggaatccaa ttttttctag ctcttctagc gcatcatact gggtttttgc
     7381 ttgcatagga ccgaaatcag ctactgtgta taaaaaagtg tccagatttc ttttcgctgt
     7441 gattttagaa tctaactgac gcaaagaacc agctgcggcg ttccgaggat tcgcaaagat
     7501 atcttttccc tctgcttctc tgtcttggtt caattggaca aatgaccgtt ttggcatgaa
     7561 acattcgcct cttacttcga tgttcattgg ttcttttaat ttgattggga ccgaccggac
     7621 agtttttagg ttttcagtga tgttttctcc tacagttcca tccccgcgag tcgcacctct
     7681 cacgaaattt ccatcttcat aacgcaaaga aacggaaagt ccatctattt ttagttcaca
     7741 gcaatagtca actacacgtc ccactgcttt ttgtacacgt tgatcaaaag ctatcagctc
     7801 ttctttgctg aaaacgtcgt tcaaactgta aagcggaata tcatgagtga ctttttcaaa
     7861 accttctaat acttttccgc ctactcgttg agtgggagaa tcagacgtga tcaaatcagg
     7921 atattgttcc tcgattgcta ctaattctgc gtattcttta tcataaacat agtcttccac
     7981 tgttggttta tcttctacgt agtattcgcg agaccattgg ttcagtcttg tacgaagttc
     8041 ttctgctcgt ttagcagctt cgtcaatcgt catttcatgt tccatcattt accctccact
     8101 actaagcttt ttcaattggc gcaaaggcag ccaaaagacg ttttactcct tgacttggga
     8161 aagccacatc tagttctaga tccttcgacg tcccgcttac tcggacgact gttcctagac
     8221 cccatttttt gtgtttgact ttatctccag gtttccagct gttattttcc cctccagtag
     8281 cacttttaga gggaacaacc gctgtctctg gctgtttata gctatatgct gtttttttag
     8341 cgaatacagg cgttttttta ggcgtaggac gcatgccttc gatttccagt aactcctgat
     8401 cgatttcttc tacaaaacga gaaggtcggt tatattgcgt ccgtccatat aatgttctgg
     8461 agaaagcatt tgttaaatac aacgcttctt ctgctcgcgt gatccccaca tatgcgagac
     8521 ggcgttcttc ctccagttcg ctttcttcca tcaatgctcg agataaagga aaaaccccct
     8581 cttctagacc aatcaggaat acaactggaa attctaatcc tttggctgca tgcaaagtca
     8641 tcaatgtcac ttgggaagca tcttcttcta aattgtcaat gtcggaaaca agtgccagat
     8701 catttaagaa gaccgttagt ttttcttctg gtgcatcagc gtcttcttca ttttgctgtt
     8761 cgaattgttt gtcgaattct tgtgtgaccg ttaaaaattc ctccaagttt tcgatgcgtg
     8821 cttgtgcttc taatgtattt tggattttta agtcttctag atatccgcta cgatccaaaa
     8881 cctctttcgt caattctgtc actgtcaagt atgggatcat ctgagtgact tcttggatca
     8941 tctctccaaa tgcacctaat tgctgtcctg cttttccacc gatatttgcc agatccacat
     9001 tttgcgctgc ttcaagcagt ggccattcat gaagagaagc aaaactgcgt agtttttcga
     9061 tagatcctgg tccgatgcct cgtttgggcg aattaacgat acgctcaaaa ctgattgaat
     9121 cttgaggatt taccagcaca ttcaaatacg ctaaaatatc tttgatttct ttacgatcgt
     9181 agaacttgtg ccctccaacc attttataag gaatatttgc tttcaacaga gtttcttcca
     9241 ttacacgaga ttgtgcgttc gtccggtaca agatggcaaa gtcgccgtaa ttccgatgat
     9301 tgcttcgaat ctcttcttgc atccggtcta cgataaaacg ggtttcgtct cgttcattat
     9361 ctgcacggta gtaagtgatc ttgtttcctt ctttgttttc tgtccacaag ttttttggtt
     9421 ttcgattgct attgttttca atgacttggt ttgctgcgct aaggatgttt ttcgtggagc
     9481 gatagttttg ttctagcaaa attactgcag catctggata atctttttca aaatccaaga
     9541 tgttctgcat atctgcaccg cgccagccgt aaatactttg atctgcatct cccacgacgc
     9601 acaggttcct aaatctacct gccaataaat tgactagcgt atactgtgcg tgatttgtat
     9661 cttggtactc atctacatga atataatgga atttgttttg ataatacgtg agtgaatcag
     9721 gatgttcttc aaataatcga atcgtattca tgatcaggtc gtcaaagtcc atacattgat
     9781 tatattgaag ctctttttgg taagcagcat aacattttgc cgcaatctct tcgtaataac
     9841 tgccttgcat ttttgcaaaa tcctgagggg tctgcaggct gttttttgct tgagagattg
     9901 ttcctaatat acttcgagga tcatattttt tcggatcaat gttcagttct tttagtatcc
     9961 gtttcatcaa ggtcagctgt tcagaagaat cgatgattgt aaagttccgg ttatacccga
    10021 taaaatcaac atctcttctt aaaatacgca cacacatcga gtgaaaagtc gatacccaga
    10081 catcttcgcc tccagatgcg agaatcgcat tgacacgttc tttcatttct tttgcagcct
    10141 tattggtaaa agtaatcgct aggatgttcc aaggattgac ttctttttcc tcaatcaaat
    10201 aggcaatgcg gtgtgtcagc actctcgtct ttccacttcc tgcacctgcc attagcagta
    10261 atggtccgtc agtgtgaaga acagcttctt tctgacgggg gttcatcccg tttaataatt
    10321 cagctttgga tgacattaac aacatccttt cttttatcaa tcttgttaat tatagcattt
    10381 tcttggcttt cattaaacta gctagttcaa aatcatttgt ttctttttat ggatttttcc
    10441 ttgtcaattc gttgatttcg cgctaaatta aaagaaagcg aggcgtttga atggaaaaaa
    10501 aaggaaaaat caagaaatta gataagactc agatatattt tattctaaaa ggaatatttg
    10561 ttggactgct gacaggaatc gtcgttagcc tgtttcgatt atctatcgaa aagctaagcg
    10621 atttagttcg aagtatctat gaaatgagtc gggaacagcc gatttactta attggcctag
    10681 ctatttgctg catccttgca gcatttttcg tgggttatct ggtcaaaaat gaaccggata
    10741 tcaagggaag cgggattcca caagtagagg gacagcttcg cggggaatta tcaatgaact
    10801 ggttctctgt tttatggaaa aagttcattg gcggtgtact ttcagttggt gcgggccttt
    10861 ttctaggtag agaaggacct tctatacagc ttggcgcatc cattggacaa ggagctggac
    10921 aattgtttcg gagtccatct tcagaagaaa agattttaat ttctagcggt gcaagtagcg
    10981 gacttgcagc agcattcaat gctcctatcg caggattact ctttgtttta gaagaaatcc
    11041 atcactcctt ttctccactt gtttggctga catcatttgc ttcagcaatc actgctaact
    11101 tcgtctctct ttactttttc ggcttgagac ctgttttata tctaggtgac cttccttctt
    11161 tgccgttgaa atattatggt tccttagtga tcttaggagt acttctaggc atcttaggtt
    11221 ttatttatca gaatgtcttg ctggctttgc caaaatttta caaccgattg cctttaccag
    11281 cttattttca cgggttcgtt cctttcttgc tgattttgcc aattggttat ttatggccta
    11341 gtactctagg cggaggcaat caaatcgttt tagcttttgg acatgcttcc ttgccaatca
    11401 ttatgattat cggtttgttt gttcttcgat tcgttttttc catgatttct tacggtgggg
    11461 gtcttccagg ggggattttc cttccgatat tgacattggg ggctttaatc ggtagctttt
    11521 atggaaacat tatggttgat ttcgggatgg atccaatcta catcaaggat tttgtcgtta
    11581 ttgccatggc tggttatttc acagcgattg gaaaagcacc gctgacagca attattttag
    11641 tgacagaaat ggttggctct cttaatcatc tgatgccttt aggattggct gctcttgttg
    11701 cttatatcgt caatgacctt ctcggcggta atccaattta cgaatcatta ttggaacgtc
    11761 tacttggaaa tactcagacc gcctcactaa aaggtgcaag gacaactttt gattttccag
    11821 taacggcaga gagttcttta gacggattaa tggtacgaga cttcaattgg cctaaaaata
    11881 tgctgctaat ctctatacgc agaggaaatc aggaaatctt gactcacggt gatacgatca
    11941 tgaatgtagg agatattttg gttatcttga ctgatgaagc aaacttgcca agaatcaagc
    12001 aggaaatcga ggctaaatct tctatcacaa atttgaagca atcattttaa cgaacatgga
    12061 aaaactattt tacaacaact attcatctac actacttgat tctatatgat tctataaaaa
    12121 aagaagaggc tgggaaaacc cagcctcttc ttttttattt ctatgcttct gctactgcag
    12181 gagcttttct caataaacca taaatcacac cagatacgac tgctccgata gcaataaaca
    12241 ccaaatacat gaccgggtga ctcagtaaga atacaacgaa aatcccgccg tgaggagcca
    12301 gtaatttgat tccgaaagct ccgactaatc cgccagttaa agcagaacca ataataaaac
    12361 ttggaatggc acgaatcggg tcagctgctg caaatggaat cgatccttcc gtgacaaacg
    12421 atagtcccat cacgatgttc gtcaaaccag catcttgatc tgttttgctg aatttattct
    12481 taaacaaacg tgtagcaacg aaaattgcca atggaggtac cattcctcca gccataacag
    12541 aagccatcac gacacttccg ccagttgcaa cagtcgtagc taaagttgct gtaccaaaca
    12601 cataagcggc tttgttgatt ggaccgccta aatctgccgc catcattcca gctaataatg
    12661 caccaagtaa agcagcattc gaaccactca tacttgtcaa gaaaccgttc aatgcatcat
    12721 tgattgcttt catagggaca ttgaccagca acatcaagaa accagtaatc agtaagccaa
    12781 acactggata aaagaggatc gttttaattc catccaatga ttttggtaag ttcttgaata
    12841 atttacgtaa aaatacgatg acataaccag ctaagaatcc accaatcagt gcacctaaga
    12901 atcctgcacc gcctgtatta gctaatgcac cagcagcaaa tccaacgatc aaaccagggc
    12961 ggtcaccgat acttgaagca ataaatcctg ctaataccgg cagcatgaaa ccaaatgcag
    13021 cttgtccgat ttggttgaac cagctagcag cttgattgta attccctaga tttgataatt
    13081 gatcatgtgg cacgccaata aattggtcga tcatgaaaga aagcgcaata gcgattcctc
    13141 caccaataac gaaaggtaac atgtgtgata cgccgttcat caaatctttg taaattcttt
    13201 gtccgatagt accatccgca tttccttctt ctgaactgct ttcttttcca tcaccatgga
    13261 atactggtgc agtaccacta agggctagat tgatcaactc ttctggttta cggataccat
    13321 cactgacagg acgattgatc agttcttttc cgtcaaagcg attcatctct acttttttgt
    13381 ctgctgcaac gattacacct tctgcacggg cgatgtcttc agcagtcaat cggtttttga
    13441 ttccttctga accgttcgtt tcgactttga tctccacacc catttctttc gcttttttct
    13501 tcaaagcatc ttctgccatg taagtgtgag cgatccctgt tggacaagct gtaacggcaa
    13561 caacgaattt tctttctgga tgagcagaag cttctgcagc ttgtgtttct tgttctgctt
    13621 tttcttctgc ttctttttgc tcttcagctg tttggaacaa gtcttggact tcttctggtg
    13681 tatttgcttc ttttaatttt cctacgaaat ctgcatcaat cagcaatcga gatagtgctg
    13741 ctaatgcttg caaatgtgtg tcgtttgcgc cttctggagc tgcgatcatg aaaaacagat
    13801 aagttggctg tccgtctaag gcttcataat ctactccttt ttggcttttt gcgaataaaa
    13861 ctgttgcttc tttaactgca ctgtttttcg cgtgaggcat ggcgattcca tcaccaagac
    13921 ctgtagaagt ttgtgcttcg cgggctagaa ttccttcttt atatgtttcg atatcggaaa
    13981 tccggccggc atcaaacatt ttttggacca tttcatcgat tgcgcctttt ttatcagtcg
    14041 cttgcaaatc catgatcatc gcttctttga ccattaaatc cttgatattc atgattatcc
    14101 tccttgaaac taatcaattt ttgtgatttc aacttctggc agcaactcat caatcaattc
    14161 gcgagctgcc aagtcatcag aaaaagctgt tgcgcttccg caagcaactc cccatttaaa
    14221 ggcttcgatt ggatctttcg tacggctgta agccccaata aagccagcaa tcattgaatc
    14281 ccctgcacct actgaatttt tcaatgggcg aactaataca ttcgttttat aagttccttg
    14341 ttccgtgaag agcaaagcac cgtctccagc cattgagatc agcacattct gtgccccttc
    14401 agctagtaat tttttcccat acggaatgat ttcttctaca gattggaagg ttgtgttgta
    14461 aagctcagct aattcgtgat tgtttggttt gatcaacaac ggtttcttaa caagtgcatt
    14521 ttttaggtca tcacctgttg tatcaatcac gaaatctgca cctttttctc gaatgatatc
    14581 aatcagttct tgataaaatc cttttcgcag gctagctggc gcgcttcctg acaatacgac
    14641 gatatctcct tgtcctactg ttcctaatgc ttgcttcaac tcatcgattt cttcatctga
    14701 aatagatggg cctaagccat tgatctcagt ttcagtgtct gattttagtt tgatattgat
    14761 tcttgtatct tcttttacgg ttgtgaacaa agactggatt tgttcttttt caagccactt
    14821 cgaaataaat tcgccagtga accctcctaa aaaccctagt gctgtagatt gagaatcgat
    14881 ccgtttcaaa atacgggaaa cattgatccc ttttcctcca ggtaacttga agtcttcctt
    14941 cattcggttc aaatcgccta gttttaaccc atctacgcgt acgatataat caattgatgg
    15001 attaagtgtt actgtgtaaa tcattgtatt gcctccttaa ttgttgtctt ttcacggaat
    15061 tcattaacga attctaatgg acaacgctcg gttatgatcg tggctgcttc caaaggtgca
    15121 acagaggtga aggacaactg gttgaatttg gtatgatcaa tcaatacaaa tgtctcttcg
    15181 gaatttttca tggctaaacg tttaacagct gcctcttctg gatccggagt cgtgaatcct
    15241 tgttcaagat gggcaccgtt cattcccata aatgctttat taaaatgaaa attttttagt
    15301 tgttctatcg cattagatcc taaaacagca ttagttgcca gctttatcgt tccacctaaa
    15361 ataatggtag caagctccaa atcaactaaa tttgccgcat gttgaacgga atttgtcact
    15421 actgtgatct gttttccttg cagaaaagga atcatttcta acgtagtaga gccagcgtct
    15481 aaataaatga catcttcgtc attaatggtt tcagctgccg atttggcgat ttgctgtttt
    15541 tcgtgaacgt ttttgagtga tttttcactc atattctgct caaaattcaa taattgctct
    15601 ttcttagctc cgccatgaac tctttctaga aacccactat tctccagctc ttgtaaatca
    15661 cgtcttatcg ttgattcaga agcattcaaa aggtttacaa gatcttgtga tttcacgatt
    15721 ttctgctgtt caagcaagtc taggattttt tgtctgcgtt cctctgtaag cattttcacg
    15781 atcacctcaa tacaaataat agcatatttt ttttctacta gcaatataaa tcagtcaaaa
    15841 acgttcataa aaattataaa tcagtcaaaa aaaaataaaa aaccagtcag aaagactggt
    15901 tttttatttt tttgtctatc atcaagccgt tttatcgttt tttattttcg cttcttgttt
    15961 ttttcatata aacagaagca ggttttcttt ccttatgaag aagataataa taaagtccca
    16021 tcccgccaaa catgaccaaa aaaccgatga ctgatcgaat aggaaaatta ggaacaaaaa
    16081 agagacagac ggataataat gatgccggaa ccattattcg ataatagagt tgtttatcca
    16141 ttttcttcac ctcatatttt ttgtaaagta atcaattgtt ctaattctcg gtaatctaaa
    16201 aattgcacac gcagatgctg ctcacctttc ttgacatcga attggatact tcgccgcttt
    16261 ttctttattc gtttgaaggt aacatcagag agtctatgtt tgatttcctt taacggtaat
    16321 tctgacaggg attctgaagg aacatagatt cttaaggata aaagatactc ttgatatttt
    16381 tcatctgtta cctctacttc tagatattcc ctaatataat aacaataagc ttcgattcca
    16441 ttatccaaag caacagtcgg atcgataact ttatagcaca tcatgcgtga tcgattctcg
    16501 tcaacccaag gaaaatacgc ctgagcattt agtgttccgc gaacacgcct ttctttataa
    16561 tctccttgtt ggatatcata actttttgtt acaaaaaatg aagcattctt tttaggaaca
    16621 ggttgcattt gtgcattctg atactggaca gattggatcg tatggtcagt gtaaatgtat
    16681 tcaacgaatg gcggtttcaa taaaccgaca ccattgtccc aagcaagatg atcaaaccca
    16741 agctggtccc taaaatagga ttgaaacatt tgattgtttg gcttttgcca gttaaagggg
    16801 tctcgataaa gctggataca tatgacttcg ttttctaatt ccttcacttt ttctacttgt
    16861 tggatgtcga gaaaaatcgg tttagggatg atttgatgat aatagcgact ataatacatt
    16921 tcccaacacg aagtgaaaca taaaccttgg taaaaaagat cgtatcctgg aaattggttg
    16981 caatcgacta ctacttcccc gttttgttgt ttcattttag gtaaatgttc gatttcatcc
    17041 tttttctcaa acaatagccg ttgagagatc tgttttgtat tgtgatagat aaactcgcta
    17101 taagctcgca tataggcgaa caccccatga gcaatcattt ttttctgaat gaagtctcga
    17161 atcaaaaatt gcttttcttt gaaaacagag atagacaata cattttttat caataattga
    17221 ttatcttcca agcgaaaact acattcgttc tcgtcatttc ctacaactaa ccaactttcg
    17281 tctcttaccg atgctagcat tattttttct tgaagctttc gaagatcata cggatccgct
    17341 tgttgtgtga aagagcggat aaatgttggc tggatcaaca gacgtgtcaa aatagcccaa
    17401 tcgcttattt cctgtttttt ttcttcttca tttaagtctg tgagctggat agtcatcaat
    17461 aatttatctt cgccaatcat tttctcacct ctttcaaatc tttttataat ttaattataa
    17521 ccaatgataa agaaaaacta aaactttatg tcttagaaac caatttggac gattacatga
    17581 tagactaagt aaaaagaaac aaagaggtgt ttacaatggg gttgaaattt gaacgtacag
    17641 ataatttaga taaacttttc gaatcatttg ctgtcgatcc ggaaaaaaca gttgcaccaa
    17701 aagaagagat cgatcgattt ctaaaaccac agccacaaaa tgaaaagaaa aaagacaaga
    17761 aacaaaagta agttaagctc aaaaaaagac ttaccagcca aacttttatg aaagagtttg
    17821 actggtaagt cttttttatt ctttttatga ttcaattgct tgttcgattg ctgcccacgc
    17881 ttcttctttt ccctgttttg taacggaaga aaagagaata aacgtgtcat tgcgatcaaa
    17941 atccaacgct tttttgattt cgctctcatg cttgttccat ttaccacgag ggatcttatc
    18001 tgccttagtg gcaacaacaa ttacagggat atcataatac ttcaaaaatt gatacatctg
    18061 gatatcttct tgagaaggct tatgacgcaa atctactaaa gaaacgaccg cacgtagctg
    18121 gtctctagtc gttaaatatg tctcgatcat ctttccccat tttgctcgct cagttttgga
    18181 tacttttgca tacccatatc ccggaacatc gacaaaatgt aatgcgttct cgatcaaata
    18241 aaaattcaat gtctgggtct ttcctggctt accggaagta cgggctaaat tctttcgatc
    18301 aatcaatgtg ttgataaaag aagatttccc tacatttgat cgtcctgcca gagctatttc
    18361 tggcaattgt gtatctggat attgttttgg tgccactgca cttatgataa tttcagcttg
    18421 atggactttc atggtctctc tccttaacaa ggtgatgaat aaagcaagcg gctgtcgcaa
    18481 aagccaaaat gacttttacg gcagcctctt ttcatcaacc cgcttttttc tctgaatctt
    18541 tatagattac tgttggctca ccagacaatt cagctgcttc ttttgtgata atcacttttt
    18601 cgatgttttc atttgaaggg atatcaaaca tcacatccat catgatttct tcaatgatgg
    18661 aacgaagtcc tcgagcaccg gtattgcgtt cgattgcttt tttcgcaatg gctctcaacg
    18721 cttcaggttc gaattcaagt tcggtattat ctagagaaag aagtttttga tattgtttga
    18781 ctagcgcatt tttcggctca gtcaagatcc gtaccagatc atctgttgtc aatttctcta
    18841 aagctgccat aacaggtaat cgtccgataa actcagggat caatccgaat tttagtaaat
    18901 cttcaggaat gatatgctgc atgacacttt cgtcttcaga aagtttttgg ttttgtgtac
    18961 caaatccaat cgtcttttcg cccatacgat tcttcacaat cgtttcgatg ccatcaaatg
    19021 caccacctac gatgaacaat atgttcgtcg tatcaatctg aataaattct tgatgtggat
    19081 gtttacgtcc gccctgaggt ggcacacttg caacagttcc ttctagaatt ttcagcaagg
    19141 cttgttggac accttcgccg gaaacgtcac gagtaataga tacattttcg cttttacgcg
    19201 caattttatc gatctcatca atatagatga tccctttttc tgcaagttcc acattgtaat
    19261 cagcagattg aagcaattta agcaaaatgt tttctacatc ttcccccaca taaccagctt
    19321 ctgtcaaact tgtcgcatct gcgattgcaa aaggtacatt caatgttttt gctaatgttt
    19381 gtgctaaaaa tgttttacca gaccctgtag gtccgatcaa gcagatattg cttttttgca
    19441 gttcaacatc atctgtgttt tcttgtgctt gctgattgac acgtttatag tgattgtata
    19501 cggcaacagc taacgagcgt tttgcgcgat cttgtccaat cacgtagtta ttaaatactt
    19561 caagaatttc tagtggttta gggacttcag tgaattcgcg tacagcttct tcataaaatt
    19621 cttcatcaat gatttcttta cataggtcga tacattcatt acagatgtag acccctggac
    19681 cagctacgat ctttttcact tcttcttggg ttttcccgca aaaagaacag cgtacggtct
    19741 cattgctact tggattgtca tacatggtct tcacccctta acttgcaaaa agtatgcaag
    19801 aaactatcaa agactttaca gtcttttact atcataacat agtttagtaa aaagaaaaag
    19861 tgtttaacaa aggatcaact gacctatacc tttgcatttt ttactcttaa caaaaaataa
    19921 ggaactgtga caaaatcgtg tcacagtccc ttatttcgaa taaacaattt tctagaagta
    19981 gcttcttcag aaatgattta ttacttgatt tctattcttt cgcttctgtc gctgtacctg
    20041 tgatcaattc gattgctttt ttcattgtca catcatgttc cagcatatct tctgtcaaca
    20101 cgcgtttgat ttgttcgatt ggcatgttgt atgtttctga taattcttgg atttctgcat
    20161 tgatttcttc ttctgttgct tgaagatttt cagcagctgc aattgcttcg atcacaagat
    20221 ttgttcttgt gcgcatttct gcttcacctt cgaattgttt atgcaagtct tcttctgtag
    20281 aaccagttaa ttgatagtac atttcaggag agataccttg acgttgcatg ttgttcaaga
    20341 attcgtccat tgaacgatgt acttcatcat gaaccatcac gtgtggcaat tcaacgattt
    20401 ctgcgttttc aacagccata cggatcgctg cttcatcttt tgcatcgtcc gctgcttttt
    20461 cttttgtttc tactaattct ttgcgatatt tttctttcaa ttcgtctaat gttgatactt
    20521 cgtcatcaac atcttttgcg aattcgtcat ctaattcagg cacttctttt gcttttactt
    20581 catggattgt cactttgaag actgcttctt ttccagcaag atcttctgct tgataatctt
    20641 ctgggaaagt tacatttaca tctagacttt cacctgattt atgaccaact aattgatctt
    20701 caaaacctgg aatgaatgag cctgaaccta attcaagtga gtaattttcg ccttttccgc
    20761 catcaaacgc ttcgccatct acaaagcctt cgaagtcgat tactactgta tcgccatttt
    20821 cagctgcagc atcttctttg ataactaatt cagcttgtgc ttcttgttca cgtttgatac
    20881 gtgcatccac gtcagcatct gttacttcac ggtcttgttt ttcaacaact agatctttat
    20941 attgtcctaa ttttacttct ggtttcactg ttacttcagc agtgattacc cagtcttgac
    21001 ctttttccat gctttcaaca tcgatttttg gttgagaaac aggatcgatg ccagcttctt
    21061 tgacagcagc ttcataagct tctggtaata cagcattcaa tgcatcttca tataaagctt
    21121 cttcaccgta catacggttg aatacttgac gagaaacttt ccctttacgg aatccaggta
    21181 cgttcaagct ttttttcact ttattaaacg cagttgttaa tcctttttgg atttcgtctt
    21241 gcgcaatcgt aaattttaaa acaccatcat tcgtgccttt tttttcaaat gttgcagtca
    21301 tttaattccc tccgagttta gaaattcatt ttatacatca aaaacgtttc atgcatacct
    21361 ttaaatagta cactaaccct ttcaaattgt aaacatattg aatggataga atccttgttt
    21421 ttcataacat ttttatcttt ttcactgata aaatctaatg gaagaagatc atttgctgca
    21481 aaattttttc tctttttttt aaaaaatctt gtttttcatc ttctgtacct tcatacatcg
    21541 tttttcctgt acacgcctct acagtcagtt gaaaccagcg cttcgggtct tcgatgtatg
    21601 tatcatagac aggataaaca attgcagatt gcaacgtgaa ttcttctttt aacacggaac
    21661 taagcagcgc atcttcattc ccgtaacgat ctgaaaatac acataattgc tggtaacttg
    21721 cctgctgctg cggctttggc agatcttttg gtatgacttt gactaacttt tcttcaatcg
    21781 tcaaatagaa aacaggatgg cattcgttca attgcctaag tgtctccagt aattttgccc
    21841 ttgctaaaat atggacctct ggcgatacaa taagtttact cgctgtttgg ataaactcat
    21901 gataaggaag ctgttcgatc tcatcaatag acgcaagctg acgtgcaggt tccaacgaag
    21961 gaagttcctc cgatttattg tggagtgcct tgatcttctg cctctccatt tgtccggata
    22021 attcttctgc aaattggatt ttttcaatga cccgctgctc gaaagcttca taaaaatctg
    22081 tacttgctat caattttcta gcatataaaa aatcttgtgt actgattaac aagtcaaaat
    22141 aaaattcagc aaactcctca ttttgaagat actgtttttc atgcaagagt gcttgcttca
    22201 atgcttcttt tttctcgtcc aactcgtaaa gacaaaaaac aagcaggcta ttagcctcaa
    22261 agctttcttt caactcgtaa gctcgttgaa ataattcttt tgcctgctga tattgctggt
    22321 ttgctagtgc ttgttgtgcc actcgcatta aattctcata tttttctgga aaatcaatcg
    22381 gcttcacgca gtgtcctcct cttgctcata ttcagctcat gttcaatagt agcaaaacaa
    22441 aaaaagcatg acaattaaaa ataactgaag caccctctaa aagctagatt ttagatctga
    22501 cttttagaag gtgctttcaa ctgttactgc tttttattgc tcatcagctg ttgacttcct
    22561 ggttgaacgc tttgatcaga ttgatcgtaa caacacttgt attagtgaaa aaatcgccca
    22621 gatgttgctt tttctgatta aagactgctg gaagataccc taacatcagc aaaggaaatt
    22681 ttagaatgaa ccggcaagca ccttctctaa ctaaaacggt tgaccagcta agttcttctt
    22741 catcaaagct gatgactcga ataccaaaga tcattttccc aatcgtttgg ccatgattga
    22801 atttcgtcaa taaaataaaa tacgctagat aaataagtaa ggctgtgaat ccgtagagac
    22861 tcagataaga atctgaaaaa ttaagccaac ctagattttt ggcaatacca agtgttaaac
    22921 gcgtaatagc tccgatacac aaaagatcga ccaagaaggc aaacattcgg ataaaaaaac
    22981 ctgcatagaa atacttagga aaatcatttg cttttggcgg ttcatttctt gtgtccgtat
    23041 actttttcca tttacgttgt ttctttttga tttcttcggg cgtcaaaggc tctctttcaa
    23101 aagagctcag taccttttgg gaaaagttgg agtcaggtcc tacagcaggc tgctgttgct
    23161 tatctttttc ttgttcctta ttttcatttg aaagctctga catgcttact cacctccgta
    23221 ataatacatt gcttttggtg attccgctgt ccctagattc tcaagaacgg ataagatctg
    23281 actagtctca gaaggcttca atccctggaa ttctgctagt ttacttccta accatgtact
    23341 agcaaaccca gatggcgtgg aagaatattc tactagttcc gcatcttcaa gctgttgttc
    23401 ctttcgtata gcttttaaag catcttcagg aaatcccaat tcatcgacta aacctacttc
    23461 ttttgcttgg acaccatcat aaatccttcc atcagcaatt tttttgactt cttcttcaga
    23521 tttattccgt ccctcgctga cgattgctac aaaacgacta tatgcgctgt cgatatatgc
    23581 ttgaagcacc gcttcttctt cttttgtctc tggtcgcgtt cctgaaccca tatctttcaa
    23641 cgctccgctt ttaactgttg tatcttccac acccagtttt tctagaagtc ctgaataatt
    23701 caatcctgac atgatcacac caattgagcc ggtcactgtt tcttctgttg caaagatttt
    23761 atctgcttga gcagaaatat agtagccacc gctagctgcc atgtttttca tgctgacata
    23821 cattgggata tcatgttctt tacgaatcgt atccaatttc ttagcgattt ccgcactttc
    23881 gtagacaccg ccaccaggag aatttacttc taaaaagata cctttgactg ttggatcttc
    23941 ttcaatcgcc ttcagttgtt ccataaaatt tttatgattg taaccttcag aagcaaataa
    24001 acctgaagac cctcccgcag caatcgttcc atctactgat aattttacga ttttttgcgt
    24061 actgtctccc tcttccaaca catttggagc tagttcttca tccccatata accaactgtt
    24121 caatccctca atttgatcgt tttcctgagg cttagtcaaa ctagctgaaa ctaacgaaac
    24181 cacaaataat cctgcagcaa tcagaactgc tgcccaacgt cttttattca ttctttctcc
    24241 tttctaatac aagtttttta tgacgatcac agtttctgac tgctcagaag tgtcgaaccg
    24301 ctctgctttt tcttcccgcg catacccgga aaacgactct tttgttaatt catcttctac
    24361 cgtaaactca cgagcttgag caacttccaa ggcatcaggt gcgatttctt ggatgctatc
    24421 ccagccagta tctaaaaata ttttcgtatc atctgataca ccagctgatg cttcgaattt
    24481 ggacaattgt ttttttaaca ttcccagtgt catgatctca atttttgaat ccatttttac
    24541 tcctcctcta tccgattact ttctattgta ccgaactcct gaaaaagatg atagataaaa
    24601 acccattatt ttttgacata aaaaggacat ttgacaaaga atttgatata taccaattta
    24661 ttttttattc tttttttgtt gacaagaaca ttcgcaaaag gttatgattt taaatgtttc
    24721 cagataaaaa acaaaaagga ggatagttat accttttagg atagcgccag gtctgtgtaa
    24781 acagatgacg aggagagggt ttatcgaaag attcggcgga tggccctagg gactgcactc
    24841 tacagaaaat ccacaaaaaa tgtctgcaaa gataaaacaa aaggattttc ctgcagctgg
    24901 aagcattcat acgaattcat tatagaaagt aggatgtttt ttatgtgcgg aattgttgga
    24961 atgattggat taaaaaatgt aacaccagga ttgattgacg gtctagaaaa attagaatac
    25021 cgtggttatg attcagctgg tatctttgtc tcagatggca caactgatta tctggtcaaa
    25081 gcacaaggca gaattcaaaa tttgaaaaac aaaataaata ttgatactac aggaaatata
    25141 ggtattggtc atacacgatg ggcaactcac gggcaacctt ctgaagaaaa cgcccatcca
    25201 catacttccc aaagtggacg cttcgtgctc gttcacaacg gcgtaatcga aaactttgaa
    25261 gaacttaaaa ttgcttattt agcaaatgac cactttattg gtgagaccga tactgagatt
    25321 atcgctcatc taattgaaac tttcgcgaag gatactacaa cgcaagaggc gtttttaaaa
    25381 gctttgcggg tcatcaaagg ttcttacgct ttcgctttga ttgatcggac agcacctgat
    25441 gttatttacg tcgctaaaaa taaaagtcca ctattaatcg gactcggcga tggattcaat
    25501 gtgattgcca gcgatgcaat ggctatgctt gctcatacga aggaattcgt tgagattgaa
    25561 gatgaagaga tggttaccgt cacatcagaa aaagttatta ttcaaaattt tgcaggtgac
    25621 atcgtagaac gttcttcatt cgaagcacaa gtagatgctt cagatatcga gaaaggtacc
    25681 tatccttttt atatgctgaa agaaatcgac gaacagccta ttatcatgcg tcgtatcgct
    25741 caaaaatacg taacagaaga caatcgtgtc tctcttgatc aaaagctggt cgatacattg
    25801 tctgatagcg atcgaatcta tatcgttgct tgcggaacta gttaccatgc ggggttagca
    25861 ggtaaacaga ctttggaaaa attgacacag attcctgttg aggttcattt agccagtgaa
    25921 tttggctata acacacctct tctttcaaaa aaaccattct ttattttctt gagtcaaagt
    25981 ggcgaaacag cagacagccg ccaagtgctg gtaaaaatca accgtctcgg ctacccttca
    26041 cttacgatta caaatgttgc tggttctact ctttcgcggg aagcatcctt tacactgcta
    26101 ttgcatgctg gacccgaaat cgcagtagca tcaacgaaag cttatactgc acaaatagct
    26161 gtactaactt tattagccaa agctatcggt gataaaaaag aatttgaaac ttctcttgta
    26221 ttcgatatca ctcatgaatt gagtttagtt gctaatgcga tggaatcggt catcgcccaa
    26281 aaagattatt tagaagagtt ggcagaagta tatctcagcg atacaagaaa tgccttttac
    26341 attggacgtg gtgctgacta caacgtttct cttgaagctg ctttgaaatt gaaagaaatc
    26401 agttatatcc aggcagaagg ttttgccgct ggcgaattga aacatggaac gattgctttg
    26461 atcgaagaag gaacaccagt gatcggaatc atcagtgaag aagtgactgg tgcgcatact
    26521 cgaggaaacc tgaaagaagt cgaaagccgt ggcgctaaaa cactagttat tgtctcagag
    26581 ggattagaaa aggaaagtga ccagctggtg ctaccaactg tccaccctta tctaactact
    26641 ctaaccacag tcgtaccgac tcaattactt gcatattacg caacgctatt acggggattc
    26701 gacgttgaca aaccacgtaa tttagcgaaa tcagtgacag tagaataata aaagaacaag
    26761 gaccagtgtc agacatctga cactggtcct tgttctttta ttttttatga aaatatctct
    26821 taatccagtc cgatttcttc tttgactact gccgcgattt tatcgacata gtaattaact
    26881 ttctcatcag taggcgcttc tgccatcact cgaagtaatg gttctgtacc ggaaggacga
    26941 actaggattc ttccttctga tcccatttca gcttctgcct cttcaatcac ttttttgatt
    27001 gccggaacat ccatcgcacc gtttttatca cttacgcgga tattcactaa tttttgcgga
    27061 tagattgtta cctctgctgc aagctctgat aatttcttac ctgtctgttt catgatattc
    27121 agtaactgaa ttcctgaaag catcccatct ccagttgtgt taaaatctaa gaaaatcatg
    27181 tgcccagact gctcgccgcc aaagttataa tcgtttttgc gcatttcttc tacaacgtaa
    27241 cgatcaccaa cttgtgtaac gacatctttc aaaccgattc cttccaccgc tttgtggaat
    27301 cctaagttac tcatgacagt cgtaacgatc gtatcttttt tcaatcgatt tttttctgcc
    27361 aagtatttag cacagataaa catgatttta tcaccgtcga caatgtttcc aagttcatcc
    27421 accgcaataa tacgatcgcc atcgccatcg aaagctagtc cagcatctgc tcctttttca
    27481 acgaccatct cagctaaacg ttctggatgg gtagagccta cgccgtcatt gatatttaac
    27541 ccgtctggat ttgtgcccat tgtataaaaa tctgtttcaa gatcggcaaa caaacgatta
    27601 acacttgttg aagttgctcc gtttgctgca tcgacacaaa tggtcaaacc ggaaaggtca
    27661 ccgttgatcg tttgtacaag gaattgagaa tatttcaata agccttctgg aaattcttct
    27721 acagttccca atccttcagc tgatggacga ggcaattcat ccacttcagc atctaataat
    27781 gcttcaattt ctagctcttg gtcatctact agtttaaaac catctgcacc gaaaaatttg
    27841 attccattat cttgtgctgg attatgagaa gcagaaatca tgacccctgc gcttgctttt
    27901 tgcaaacgtg tcagataagc aacacctggt gtagagatca cacctagctg gaacacttcg
    27961 atacccaccg ataaaagtcc tgaaatcagc gcttcttcta acatttgacc tgagattctt
    28021 gtatcccgcc ctactaacac gcgtgggcgt tttccttctt cttcatgctg gcttaggaca
    28081 taacctccgc accgtcccag cttaaatgca agttctggcg taagttcctt gttggcttca
    28141 ccgcgtacgc cgtctgttcc aaaatattta cccatagttg taaaattact cctttaactt
    28201 aattttctga ggaatttgtt ctgctcattt gtgttgattc gcttgtttct gatgtttctt
    28261 ctgtacttgt ggtcgagctg cttgtcccag attccgaact ggaagaaatg gttgttgtcg
    28321 atggttccgt tcctgaatca gcagatgaaa tacttctact cgtacttgta ttttcttgtg
    28381 tccgactgct cgtcgaagca gagctactgc ttgacgtttg caacacgggt gtaatctcca
    28441 cactaactga ttcgggcttg atacttaacc cctcttctac tggtatttca attttacgag
    28501 ttgttcgatg attgatacct gagacattga tcggtacagc aatactgtca ccgatcgatg
    28561 caagcaaatc gctagagccg cttacttctg cttccagttt tgaaaaactg aaatcatagc
    28621 tttctacccc ggcagctggt gttccttgct gtatcccata aagtcgtacc gacttgcttg
    28681 gcttgatcga atcagctctc actgttactt gcactggatc agagacgata cttaatactt
    28741 cccctgctgc attcagcgcc tgtattggga cctcactatt aatcccatca tctgtgacca
    28801 tcgaagcgtc cacatcagct actactcgtt tgatttctgc caatgttttg tcacctgtag
    28861 tgatagagac ttttttagga ctaacggaag tatcatcaag ttcatatcca gatggtgtga
    28921 acgttgaagg aatcaacgtt tccacatcga aatcttttat tacctttttc tcaaccgtta
    28981 ctgtaatagt cgatggttcg atctttgctg tgacagcact gctcaaattt tgaattttta
    29041 gcggaacttc atgtgttcct tcgcccagct tagtgatatc tgctgtgacg cggaacatcc
    29101 gtgtatcttc gtttgcctct gcattcaact gaactcggtt tgcactggaa agtttgacag
    29161 tcactgcatt ttcatatcca tggatatagt agcgctttga atcataagaa atcttgatcg
    29221 gcacccccgt aactgtttga ttatacgctt catttccaga aagcgtattt ggaacggact
    29281 ggccatttgc attgaagtac agcgcaatac tgaacagcaa tgcaataaaa ctgtaaagaa
    29341 tgcttgtccg tttctctttt gagatcattt gcgatcccct cccttagtca taccgtctag
    29401 gaaagattga atcacgtttt tcttgttttt tgttttctct tcagcaatta gttctctacg
    29461 caagattgcc aaatattctt gctgggataa gccagccatc agttcattat ctaacgtgat
    29521 gctgacgtcc ccggtttctt cagagacaac gatggtaatc gcgtcactga cttcgcttat
    29581 accgacagct gcccggtgtc gagtcccgaa ttctttaggg atcaacatac tttctgatag
    29641 aggaagatag gcgcttgcta ctgcgatctt tccttctttt acaataactg caccgtcatg
    29701 cagcggtgta ttaggaataa agatattgat cagcagctcc ccagtgatat ctgcatctaa
    29761 agcaatccct gtctcaatat actcatctaa tccagtatga cgttcgatag tgatcaacgc
    29821 accgattttt cgcttggaca tatattggat ggctttgtca aaagacaaga tcatcttctc
    29881 atcttcctgc tgttcgcttt ttgattgttt gaaaagcgaa ctgcgtccaa gatgttccag
    29941 tcctctccgg atttccggct gaaaaatcac gatagcagct atcaccccgt acgtaatgac
    30001 ttggttcatc agccaagaca aggtgtgcag accaatgatt tccgctacga ttcggataac
    30061 aataaaaata gctacacctt tcagaagctg aatcgctttt gtgcctcgca gcagttggat
    30121 cagtttgtat acaagatacc agacaaccag aatatcaagt atgttcacca aaaaattcag
    30181 agagaacaag tcaccggaaa acatttgctg ccaatagtcc aaattaaaaa gttccccgat
    30241 ttgaaatgac ataaaaagcc tctcttctct tttgagattt cctctctagt atatcatatg
    30301 ttttaatttt agaggctgat atgctcgtat ttttcttgag aaaaacgcat aattttcaag
    30361 aaaattgcaa gtatgcgttt tatttttctc tgcaataaat atttttaaac ttcagagaat
    30421 tattcattat attttttctg ttatttttcc tttttttctt ttgtagcttg ttccattttt
    30481 ttacgtcgtt ctgctctggc agctcttcta gcagctcttc tttcttctgc ttcaaggtcc
    30541 gtcgtttttt cttttctagt cttaacgggt acatcgatag attcctctgg ttcaagtcgt
    30601 ttcttgattt tttctaattc tcccgtcagt gcagcatagt tgtaaaaacg tgttttagcg
    30661 tcgtggacag tcttttcaaa taaagctttt gcaagatttg gatttgcgtt atctagcgag
    30721 gaaaagcgaa cttgtttcct catgaaatca ggcatagcag agaaatcagg ttttttgtag
    30781 tccaaagtca ttgggttctt gtttttttcg accattgtag ggttatatcg atataatgac
    30841 caataaccag aagaaacagc ttcttttgct tctttaagcg attctttcat tccgccaagc
    30901 aagccatgtg tgatacaagg cgtataggca ataatcagag aaggaccagg atacttttct
    30961 gcttcttcaa atgccttgat cgtttgcatt tgattggcac ctgaagcaat ttgcgccaca
    31021 taaacatttc catatgtcat cgccatcatt cctagatctt tctttgaagc atactttcca
    31081 ctagccgaaa atttagcgat tgccgaagct ggtgttgctt ttgaggtttg accgccagta
    31141 ttggaataga cttcgttatc caaaacaagg atattcacgt ctgctccgct ggcaagtaca
    31201 tgatcgatcc cgccaaaacc aatgtcgtaa gcccatccgt ctccaccaat catccattgg
    31261 ctgtttttta cgaatagatc ctttctcttg tatatatcct ccagttcagg gaaaattgat
    31321 cgttcgcttt cgatcgcagc ttgcagtttt gctgctcttt gctgcgttcc ttcactctca
    31381 aacatgtgta ctaaccagtc ttccatcaat tctcgtagtt cttctgatac ttttggcaat
    31441 acttctttga ttgaacttgc caaagcctcg cgacgagtct gtgccgctag aagcatacca
    31501 taaccaaatt cagcattatc ttccaataaa gaatttgacc atgcaggccc ttgaccacat
    31561 tcattggtcg tataaggaga agcaggagaa gcaccgcccc aaatagaaga acagcctgtc
    31621 gcattcgcga tcatcattcg atcaccgaac atttgtgtca ataattttac ataaggtgtt
    31681 tcgccacagc ctgagcaagc gcccgagaat tcaagaagcg gttgattgaa ttgtgtagac
    31741 aagacagtat tctttccttt gactggattt gctttttgtc ttaatgtcat tgcaaaagcc
    31801 caattcacag cttgttcttt ctgttcttca taaggcttca tcactaaagc tttttctttt
    31861 gcgggacaag cctctacaca cagaccacaa cctgtacagt cttccactga aacttggata
    31921 cggtatttta cgccgtctgc gcctcgcatt tctctcacga taaatccttc tggcgcttct
    31981 tccatttcct cttcatctgc taaaaatgga cggatggcag catgaggaca aacaaaggca
    32041 cattcattac acatcgtaca gcgatcacta atccactcag gtacttctaa agcgacgccc
    32101 cgcttttcca tagaagccgt tcctaaaggc atttcgccag ttgtcattcc atttgctaca
    32161 agtgtaccaa cagagagttg atttccttcc tgtgcattca ctggttcaag tatttcttgt
    32221 atataaccag tgttcctttt attgattttt tcaggcatct ccaacgttct ccaatggtct
    32281 gggatcgcga cttgatgaag cagttcggtc gtccgatcga ttgcttgtat gtttttttct
    32341 acgatagcca ttgatttatg tccatagctt ttcagggctt cttcttttag cagcggaagc
    32401 acctcatcaa aaggaatgat gtcagctaat ttaaaaaaag ccgtttccat tgccgtattg
    32461 atccggcgtc ccaaaccgac ttcttgggcc aatctcatgg cattgatcgt atagaatcta
    32521 atgttatttt cagccaagta acgttttaat tttaagggca gatgcgtttc taattgttcg
    32581 tcgctccaaa gagtattcaa taaaaaaatg ccacctggct tcaatccttt gaccaagtca
    32641 tagctatgca ggtaagctgg tgtgtgacag gcaacaaggt ccgcatgctc gacaagatac
    32701 gctgaacgta tcggcgtatc tccaaaacga agatgagaga cagtcagacc gccagatttt
    32761 ttcgaatcat aataaaaata gccttgcgca tatttatcgg tgtgatctcc aataatcttg
    32821 atagctgatt tatttgctcc aactgttccg tcagatccaa atccccaaaa cttggcctgg
    32881 aatgtttgcg gttctgtcaa atccagactt tcctttggtt ccaaagattg ataagtcaca
    32941 tcatccacga tccctatggt aaaccgtttt ttgcgtatgc ttgggtcttt tttcaattca
    33001 tcaaaaactg ctgagatttg gtcgggagtc acatctttag accccaatcc gtagcgacca
    33061 ccaataacag ccgggcgaag ttcagaatca tataacgcac tttgaacgtc taataagaga
    33121 ggttctccac ctgcccctgg ttctttactt cgatcaagaa cagcaattgt ttttactgta
    33181 tgaggcaact tttgcacgaa tgtttccact ggaaaaggac gataaagatg gatattcaaa
    33241 aaacctgttt tccgtccgtt tgcattcaag taatcaatcg tttgttcaat cgtctgtgca
    33301 actgatccca tagaaacaat gacttcttcc gcatcgtctg ctccgtaata agtcaccaag
    33361 tcataagaag ttcctcgtaa tcggttgatc tctttcatat atttttgaac gatttcaggt
    33421 aatttatcat aataggggtt gatcgtttcc cgttgttgaa aatggatgtc tggattctgg
    33481 ttcgtaccac taacagtcgg atgattggga ttcatccctc gtttacgaaa tgaagctaat
    33541 ttctcctgat tgatcagagg agctaattct tcatattcca aaacttcgat tttttgaatc
    33601 tcgtgactcg tacggaatcc atcaaagaaa ttcaaaaaag ggacttctcc ttcaatagaa
    33661 gcaagatggg caacaggtgc taaatccatc acttcttgta cactgctttc agctaacata
    33721 gcaaatcctg tttgtcttgc agccatcacg tctccgtgat ctccgaaaat attcaacgca
    33781 tttgtcgtca ctgcccgact agcaacgtgg aaaacacttg gcagtagttc cccagcaatc
    33841 ttgtacatat tcggtatcat caaaagcagc ccttgtgaag cggtatatgt agttgtcagt
    33901 gcaccagttt tcaatgatcc atggaccaca ccagctgctc ctgcttcgga ttgcatttct
    33961 actactttta ctggttgacc gaatacgttt tcttttcctg ctgctgacca ttgatctact
    34021 aattcagcca tggtcgaact gggtgttatt ggatatattg cagcaatctc ggtaaatgca
    34081 taagaaatat atgcggctgc cgtattgcca tccattgttt tcatttttcg catgattcat
    34141 catccttgtt catattctct cttattatct gttccattat aacgtaaaac atgccatttt
    34201 tttcttttat ttataaaaat attttacatg tatctatttg aattggtcat ctaattttta
    34261 gacaaacagt ctaaatcgtt gccttttttt gtcagacatg tataaactat aagcgaaaga
    34321 ggtgttttaa catggatata tcagtgattg atgcaacgaa aacaaataca caaaaaggaa
    34381 tcctttatgg ttcatcgaat gctcctaaaa aaatggtcga attcatcaac ttagcttgtc
    34441 cgtattgccg acaatggttt gaagaatctt atgagctatt agaagaagca gtccaaagcg
    34501 ggcaattgca gcgagtgatc aaattgttcg ataaagaaaa agaaagctta cagcgaggga
    34561 acgtgatgca ccggtatctc acaatcagcg atggccaaaa agcgatcaaa gagatcaaac
    34621 aaatattcga tactcaagac gagtggaaac atctttctct ccaagaagta gcggattttg
    34681 cagtagataa actaaagctg acagaacaaa aagacgaaca gctgagccaa gctgtcatca
    34741 acgaagctga gcaagcacat attcgatttg ttccaactgt catcttgggt aaagaaatct
    34801 ttgatgaatc tatttcaata gaggaattaa aagaattgat ccaaacaaaa taaaaaaata
    34861 aggagcgtgg gacaaaagta aagaacactt ttgtctcacg ctctaaacac gtataaacgg
    34921 cggaagcaga agcaactcct tcttatttct tggagttaaa cgcttctgtc ccagcctcct
    34981 tattccgaat aaatggtgtc caaaagcttg catctgccag attatctcat ttcttatgac
    35041 aagctaagca aattatctcc accaaataat ttattcatat ttttgacttg cgcatactaa
    35101 ctaattctta aattttcata caaaatttct caatttccct taacgagtaa tatttatgta
    35161 cggtgattct cactagtatc ccaatcactt tttaagttat cccgatttca attctacttc
    35221 aaaaatagtt gcttttatgt atgctgttag ttgattagat ggtcatttgt tgagctaggt
    35281 cgtttatttt aaacaagttg tttctttagc ttatacctat ctcgtctacc aaacgcaaga
    35341 tggcaaacac aaatgcatac gcatctgtat ttgccacttt gattcgtata aagctaacaa
    35401 ctattaactc ctctcagcta atagttgctc tcttttttat tcagattcat ttttgctgat
    35461 cacagcctag tatttcaatg gttttgaaca ctaaaccaaa ttgttccatt acaacattca
    35521 ctggctgatg aaatggatta gtagatttcg attattcaaa gcgattttct attggtggat
    35581 cgtgttccca actaataaga cggattccaa acctatcgta cagatcttct aaaccactta
    35641 taaattttgc acctatttca ggtgttttat tgcgtggata ttctattgtt acgccattaa
    35701 atggatacca attaggatta ggctctcttt ttttctgtac atctcccata gaatagttag
    35761 cactttctgg gacataatat attcttcgac catcctctaa ttcatctaat aagatatttc
    35821 gtgatcgtct atcacttgct aaatgtagac gccacgtgcg ataccatgcc atgctacctg
    35881 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
    35941 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatttct ggtcttggtg
    36001 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
    36061 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcacat
    36121 cacctctaca ttctcacata ttagttcgta atactccttt aatatcaaaa tatctactgg
    36181 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
    36241 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
    36301 ttgttaacaa tcggttcaga tatttcccag ttgattaagg taattccgta ttgtgtcaaa
    36361 acttcttgcg cttctccgaa attaagtaca gttcttatgt atccactttc tcctaatata
    36421 aagtctacct ctcttttaaa accttcctcg agatttctaa gggctatatg tggttttaat
    36481 gacattgatt gtacattttc ccccatataa tattgcgcac ttcctcgact aaaatagatt
    36541 cctagatcgt ccccaaaagg tagatcttct ctaccactag ctaatctact tctccatctt
    36601 cgttctcttt tcatagaacc tgcgctgaac tcacacccgt taaactcagc tatgacagta
    36661 tcttcaatta tgttaatctg tccatctagt tcgtattcaa tcctaaatgg aaattctcca
    36721 taggtgattt ccggttcggg tattttaggt gagattaatg caaagaaagg aaatattaag
    36781 cttggcaaca tcaaaaaaac tcctcctatg atcacaattc ccgatgtaat ccaaaatata
    36841 atttgtacaa ttctcattga tccattgtat tccattttat tccccctatt actattcata
    36901 tccgcctatc tatatcgatc ttccaatgca gagataattg cttccatacc ttgatttttt
    36961 actgagttac ctatgcgaga tatcattctc cactaaggtg catcatattg tctatctaac
    37021 atgattaatt ctccaatagg tgtatactcc tccaaaaaca acatttaatg catctccctc
    37081 gacaacataa gctaccatgt ttgctgtata tgtgtttgag tctaattcat aagcagataa
    37141 atttactgtc gctggattaa atataatcgc attcgtattt atagctactg aatttgctgc
    37201 ttcagcacca ccttttgaat gtccgatcat tgtgatttca atagtaggat ttacgtggac
    37261 agccctcacc gtttaccctg accattatag aaatcgctta cttttttgaa ctacgcaaca
    37321 aaggatacaa tgctcctaac ttcactgtct ccatttttgt ccactaagga atataaaaaa
    37381 taggcattaa ttcatttaat tcggtcacaa cacgttacct tttaaatcat tatactgtat
    37441 ctcatacatt attatcaata aactattata aaaatgcaaa aaatgcttta tcaatctatt
    37501 ttgtaattgg tcataatatc attgaaatca taccctgtat cgatttttcc tatgaatctc
    37561 gtctatttgt gattgacgaa aaagaggcaa gacagacact ggaatggtaa ttttacccac
    37621 tattttgctc caactatttg taatcgtacc cacttttgaa ataccgagat gatcatttta
    37681 attgatatgc tgattcccaa gcaaaacccc aagaaattaa atgctctatc gcatctagct
    37741 tcttgggttt tgcttatata aattccaaca tttaaatttt ttatgctgtt cggtctcaga
    37801 aatgccgttt ttgcttatat cacaatatca agatgtcaaa ttattcgtgt tgccgatgac
    37861 tttgattaat ttttttcaaa ttgtttatta agactaatgc tgtctttttg cagataccaa
    37921 tgttcgaact ttttaacatt atcatatgat cctgcaagtt ttaacttctt accattagat
    37981 gttatcaaag ttataattaa ccttgccttt ttcccattag gatcttttat tttatgaatt
    38041 ttctgcaggt aatctctcct aatggcagct atttttacct catcagtgct gataaaccat
    38101 tgatcatcca catacatact gcctgttatg ttgtgttttt ccatttcttc cgagaacgaa
    38161 aagtttaagg ctcgttctta cttccaaagt tgaatgagtc catggattgg acttagtgtg
    38221 agaattgaga acaacaataa gaatccatat cctcccgcaa acatccctaa ggctatactg
    38281 tctctccatc cataagtagg attagaagag aaaagagtca ccgtttgtaa taatatgaaa
    38341 aggaataatc cagcacaaac accaaagata acaactccaa acattgaatt tttatacaat
    38401 gaccatttat ttataggctt ttctttcatt ttacttcccc ctactgttct gttcacacgg
    38461 aatgaattca aagttcataa gccctcctct ttgaacatat gaatacgact tgctaactat
    38521 actttaaaaa agcttgtttt aagcggttat aggcgagttg taatctctat tcatctcatt
    38581 agcaaataac ttgccgaaaa gtctactgaa attaaagcag atagtggaag aattatttag
    38641 ccaggataga cactatttgt gaaacacatg tctttacttt tgaaattcta tgatgaaaag
    38701 ccttggaata cagtagctct taattggtaa tactttttcc attataaata acctcttttc
    38761 attttatatt tatcccctct aaaaataata tctaatctaa tattttaaac ttatatttcc
    38821 ctcgctgtgc taatagagca ttactaaata aatagaaact agaaaaaagc attacgcctg
    38881 ccagaataaa ccaattattt gtat
//
LOCUS       DARBEC010000018        37214 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.018, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000018 DARBEC010000000
VERSION     DARBEC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37214)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37214)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37214
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.018"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            59..808
                     /locus_tag="SCL80_000865"
     CDS             59..808
                     /locus_tag="SCL80_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1106753.1"
                     /translation="MVELTKENRLTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQ
                     SGLTTGADGKLAIDGLAPGVYQLVETQAPTGYELDATPIEFEIERSQTAVVELTKENR
                     LTPGGVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAIDGLAPGAYQL
                     VETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNPTSSGNKHLPK
                     TGETIISQFILSILGVLLVFISIKFRKKRNI"
     gene            complement(1232..1402)
                     /locus_tag="SCL80_000866"
     CDS             complement(1232..1402)
                     /locus_tag="SCL80_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1106754.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFSTPFKEA"
     gene            complement(1596..1775)
                     /locus_tag="SCL80_000867"
     CDS             complement(1596..1775)
                     /locus_tag="SCL80_000867"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106755.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            2162..3274
                     /locus_tag="SCL80_000868"
     CDS             2162..3274
                     /locus_tag="SCL80_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1106756.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            complement(3352..3645)
                     /locus_tag="SCL80_000869"
     CDS             complement(3352..3645)
                     /locus_tag="SCL80_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEG1106757.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            3813..4127
                     /locus_tag="SCL80_000870"
     CDS             3813..4127
                     /locus_tag="SCL80_000870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEG1106758.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            4148..6127
                     /locus_tag="SCL80_000871"
     CDS             4148..6127
                     /locus_tag="SCL80_000871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1106759.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            6140..6466
                     /locus_tag="SCL80_000872"
     CDS             6140..6466
                     /locus_tag="SCL80_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEG1106760.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            6482..7000
                     /locus_tag="SCL80_000873"
     CDS             6482..7000
                     /locus_tag="SCL80_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106761.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            7000..8352
                     /gene="celB"
                     /locus_tag="SCL80_000874"
     CDS             7000..8352
                     /gene="celB"
                     /locus_tag="SCL80_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1106762.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(8436..9941)
                     /locus_tag="SCL80_000875"
     CDS             complement(8436..9941)
                     /locus_tag="SCL80_000875"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEG1106763.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(10033..10623)
                     /locus_tag="SCL80_000876"
     CDS             complement(10033..10623)
                     /locus_tag="SCL80_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1106764.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            10802..12448
                     /locus_tag="SCL80_000877"
     CDS             10802..12448
                     /locus_tag="SCL80_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1106765.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            12497..13216
                     /locus_tag="SCL80_000878"
     CDS             12497..13216
                     /locus_tag="SCL80_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1106766.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            13695..14642
                     /locus_tag="SCL80_000879"
     CDS             13695..14642
                     /locus_tag="SCL80_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106767.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            14654..15574
                     /locus_tag="SCL80_000880"
     CDS             14654..15574
                     /locus_tag="SCL80_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1106768.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            15615..17072
                     /locus_tag="SCL80_000881"
     CDS             15615..17072
                     /locus_tag="SCL80_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1106769.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            17417..18031
                     /locus_tag="SCL80_000882"
     CDS             17417..18031
                     /locus_tag="SCL80_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1106770.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            18024..19748
                     /locus_tag="SCL80_000883"
     CDS             18024..19748
                     /locus_tag="SCL80_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1106771.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELRELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            19745..21196
                     /locus_tag="SCL80_000884"
     CDS             19745..21196
                     /locus_tag="SCL80_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1106772.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(21669..23822)
                     /locus_tag="SCL80_000885"
     CDS             complement(21669..23822)
                     /locus_tag="SCL80_000885"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEG1106773.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDREYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(23824..24870)
                     /locus_tag="SCL80_000886"
     CDS             complement(23824..24870)
                     /locus_tag="SCL80_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1106774.1"
                     /translation="MTQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            25020..26309
                     /locus_tag="SCL80_000887"
     CDS             25020..26309
                     /locus_tag="SCL80_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEG1106775.1"
                     /translation="MVYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDACRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            26322..29024
                     /locus_tag="SCL80_000888"
     CDS             26322..29024
                     /locus_tag="SCL80_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1106776.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            29009..29866
                     /locus_tag="SCL80_000889"
     CDS             29009..29866
                     /locus_tag="SCL80_000889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1106777.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(29937..30836)
                     /locus_tag="SCL80_000890"
     CDS             complement(29937..30836)
                     /locus_tag="SCL80_000890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1106778.1"
                     /translation="MVQLVCNCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            31005..33176
                     /locus_tag="SCL80_000891"
     CDS             31005..33176
                     /locus_tag="SCL80_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEG1106779.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPAVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            33244..33585
                     /locus_tag="SCL80_000892"
     CDS             33244..33585
                     /locus_tag="SCL80_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1106780.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(33861..35615)
                     /locus_tag="SCL80_000893"
     CDS             complement(33861..35615)
                     /locus_tag="SCL80_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1106781.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQCISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(35626..36462)
                     /locus_tag="SCL80_000894"
     CDS             complement(35626..36462)
                     /locus_tag="SCL80_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1106782.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            complement(36464..37147)
                     /locus_tag="SCL80_000895"
     CDS             complement(36464..37147)
                     /locus_tag="SCL80_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEG1106783.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLMPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
BASE COUNT        12005 a         6542 c         7633 g        11034 t
ORIGIN      
        1 ggggtatgaa ttagatgcga caccgatcga gtttgagatc gaaagaagtc agacagcggt
       61 ggtagaattg acgaaagaaa accgacttac accaggcggt gtcgtgttga caaagatcga
      121 tgaccaaagc ggggaagtcc tgcaaggagc tgtttttgag ttacaagatg ccaatggaac
      181 cgttctgcag tcaggtctaa ccacaggagc agatggaaaa ctggcgattg acggcttagc
      241 accaggagtg tatcagctgg tagaaaccca agcgccaacg gggtatgaat tggatgcgac
      301 accaatcgag tttgagatcg aaagaagtca gacagcggtg gtagaattga cgaaagaaaa
      361 tcgacttaca ccaggcggtg tcgtgttgac aaagatcgat gaccaaagcg gagaagtcct
      421 gcaaggagct gtttttgagt tacaagatgc caatggaacc gttctgcagt caggtctaac
      481 cacaggagca gatggaaaac tggcgataga tggcttagca ccaggagcat atcagctggt
      541 agaaactcaa gcgccaacgg ggtatgaatt ggatgcgaca ccagtaactt tcaagatcga
      601 aaaagaacaa gaagtggcga tctttttaac aaaagagaat agaaaagttg ctgatagctc
      661 agatataaga ggaaacaatc caacaagcag tggaaataag catcttccaa aaactggaga
      721 aaccatcatt agccagttta tattgtcaat attgggtgtg ctacttgttt tcatcagtat
      781 aaaatttaga aagaaacgaa atatatgagc taaaataaaa aattgaataa gcatagtttt
      841 taaaatcaac tagtacccat caattgaatg gaaactaccg attttagaag gggtatacaa
      901 acaaatatta gtatcggagt agagaaggtt gaacccaacc agtaaataag taaagacaat
      961 tttgtgctgc gttgagaagt gaaaaataaa cctttgtagt aatactagtt aataaaaaat
     1021 atacaaaaac actgcgaaaa tcattcttga gtttcgcagt gttttttgcc taatgactca
     1081 agtttattgg gtaatacttt gattttcttt ctacaaataa tcagcgatca gttgtaattt
     1141 tttctctagt tttaatggct ctttgtcaat aaggagaaaa acaagtcggg tcttttgtct
     1201 cggaaagctt caaccaactc gtgaataaag gttacgcctc cttgaaaggc gtggaaaaac
     1261 caagtaatcg atcaatcacc aatttcgcat ttggtagaac atgtttggtg agctgaaagt
     1321 aggtggcgtt catgtctgta actaagaatt ctatttcttt aggattagtg cagctaagga
     1381 aatagtttgt taatcgagga attttttgtg taggtaaatt ttctcgatta atttttctgt
     1441 ctcgccatcc gcggaaataa aactcagttt atcctcaacg gaaacgtgag aacgaaattc
     1501 acaaccctca acactcaaga cagaattctt ttggaagact ttggtaaata gcttctcaac
     1561 tctttcaatg tgcgaataac ggttgtcaaa gatacctaac aacttttcga aataaaaaat
     1621 aacgacactt tttcagtcag taaagaagtg attttatgtc tgacatgatt tgaaatcgag
     1681 tgccttggac ggataaaata actttgagcg ttccaatggc ttcgacagtt tttacaggta
     1741 tagcgctgct tttttaggcg tataaccatt ggcatatgat tatattattc aaagtggaca
     1801 atggtttcct tcttcttgtt tttcaccacc attaatttcc tatttccatc taccacagca
     1861 gaaccacaat ttctacagac acatggagca gggaagagaa ctgcagcaac gatcaatgtt
     1921 tttttcttct gtaaggtttc gtaagagacc tctgtaatca tcaaattttt gtctattgat
     1981 ctcagaattt ttttgataga accattcata aagcatatcg ttctctcagt tgtttatttt
     2041 gtggtgattt aatcatacaa gaaaacgata tgttttttta taaaaacatg aataggaaaa
     2101 atagttgaca agaaaatgac aacgccatat gatgttccat agtatttaca acctgtcata
     2161 gatgactgta aaaaataaaa gtaaaaagaa aggacgacaa caggtggatt tttatcagtc
     2221 tctacaacta gatccgttta ttttaaaaca aaaaatacga gaagcagctt ctaaaaaaga
     2281 aaagtgcatg tatatttgtt cattgttttt acgatcatta ttcatcgtat tgtttgccat
     2341 ttgttttatc attataatta caacattatt tgaatcgaag cataaaccat acgctgtggt
     2401 tttattctgt atgttgatga gtattcgttt tgttgacttt ggttataaga tttcccattc
     2461 gatcataggt ttagcaatcg tcatgttctc actattagta gcaccgtatg tccagctgat
     2521 caaatggtca gttatgggca tgttgattca ttttatttta ctaagcagca tattaatggc
     2581 aaccgctagt gatcccaaaa tggggaatgc ttctttgtac ggcttttcat atctctttat
     2641 tgtttattcg ttacctaaag attcgttgaa caagaatttc tttacacaaa caagtagctt
     2701 attatttttg tttttttgtt ggttttcagt tctcctttac agaaaacatc gagaaaagaa
     2761 caaagacaaa agcttgttta aagagatttt tttgaaaggc atgtattcgc aggaaaaaat
     2821 ttggatgctc atctatgcat ttggcattag tcttttgatc gttgcaggag agtatgtccc
     2881 ttttcagcga ttgatgtggg cagggtttgc attttcttct attgtatcta gctatggatt
     2941 gatgtcgatt ggtttcaaag aacgagcagt agaccgaatc attggctcat tgattggatg
     3001 tgttttattt attggtatca gccaatttat tccttttaac tgggtcggta tattaggtgg
     3061 tctagcttta ggtgtttgtt ctacctatag atataaaaca gtttttaatt cttttggtgc
     3121 tttggcaatc actgccagtt tattcggggt tccaggagca gtaacgatcc gcatttttga
     3181 gaatatcctt ggggtatgtt taggaatcat gtatattggg gtgactgaaa tacttatccg
     3241 aaaaataaga gaaaaacacg gtttgaatca ttagatgaga tcgaataaaa aataaggaaa
     3301 aagtgaagat acacatggat gataaattgc tatcattcca tgtgtatctt tttacggtaa
     3361 atcagtgaaa cgttcgactt tcatttttaa tttatacttt ttacggagtt cgtttatttg
     3421 agccatcatt tcggatttgt gatgcttttc gattgcttct tcatctttcc aacggtcgat
     3481 cagtaaaaga gattctggat cttctagtgg aaagtaatag tcatatttct cgtttccttc
     3541 ttctgcacga atcgctttga caatgtttct ttcgaccatt tcttctgcaa attttctggc
     3601 ttgtccattt gtacttgtat acaaaatatt cactgtgata ctcactaaat cccctcctga
     3661 aaaaattcta tcactagtat atcacggtgc cagaaattat tattcaagat atgatacaca
     3721 ttttttccgc aatgctacgg aaatatatat tttgaataga aaacgcttac aagatatgct
     3781 tggtttataa aaataaaagg agtgattgat ttatgaaaaa agcattgatt atttgtgcgg
     3841 caggaatgtc atcctcgatg atggcctcaa agacaacgga tttctttaag caaaaaagag
     3901 aagaggtact cgtagatgca gtttcagcta cagaaggcga taatatgatt aagaatagtg
     3961 attttgattt atttctgatc agcccgcaaa cgacgatgtt tttggataaa ttcaaaaaat
     4021 taggagaatc tgttggaaag cctgttgtta gtatcccttt ccaagcgtat gtcccaatcc
     4081 ctacaggcat ccagaagtta gcagaggtga tcgaagagaa catatgatgg aataggagtg
     4141 gatgataatg aaacagaagg aaaaattgct gttgagatat ttgattgatc acaagaagga
     4201 gtatgtcacc agtcaacgtt tagcttctga attgttatta tctgatcgga caatccgtaa
     4261 ttacttgcag cggatcaaag agttagtaga aaaaaacgga ggtaagatca ttgctaaacc
     4321 aggttatggc taccaactgc atattttaca aagactgaca tttgacttgt ttttgagtcg
     4381 gcaagaaata gtacctggat atgctagaga accgcaagga ttttatgagt ccgaagatcg
     4441 acaaaagtat atattgaacc aattgctttt ggaagatgcc attcttttta tggatgattt
     4501 ggcagaagag ctttatatca gtcgttccag tttgacaaag gacatgcaag aaatcaagga
     4561 gcgattgatt ccatattcac taaaaatcgt ttctaagcat ggtcaaggga catggatcga
     4621 aggggaagaa agaaatcgac ggcattttat catggatact ttttttggaa accattatgg
     4681 aaattcattg aaagaatatt tagggcaaag ccgttttttc ccagacatca gttttgaaga
     4741 gctagtcatt atcattctcg atgaaacaag agaagctaag ttaaaagttt cggattttat
     4801 catacagaat ctgactttac acttggcttt ggggatcaaa cggctccgag aagggtttga
     4861 aatcaaggaa ttaggcatcg aacaagaagt ttctaagcga gtagagtatc aagtggcgca
     4921 gcggatcgtt caacgtattg aggtaatcgc aggtgtatcg tttcccgaag aagaaattgc
     4981 ttatttgaca ttacatctca tggctaagtc gaatcacaaa gaaaatcaaa atgatcagga
     5041 actatcaaag gaattagcga aagttctgaa aagattgtct caggaatttc atttttcatt
     5101 agtagaggat ctccagcttt tcaatggact actcgatcat ttgtcaccga tgatgattcg
     5161 attgagccga ggtattcgca ttgagaaccc attgactgag gagataaaga aagaaaatcc
     5221 caaagtcttt gacgcagtaa aaaggcattt cagcaacatg ccggctttga aaaactatac
     5281 catcaatgaa gatgaatggg cctatttggc tttgcatctc atggcggcat tggaaaaaga
     5341 aagagctgcg cataaactac atgcattgat tatctgtgca acgggttacg gcagtgccca
     5401 actattaaag aatcgcgtag tcagtgaatt cggtaaaaat attacagtgg taagtgtaaa
     5461 aggctattat gagatcaatg aaacaacttt aaaaggaatc gacctgatta tttcttctat
     5521 tgatctttcg acaatgtttt tcaaaattcc agttttacat gtcagtattt ttttgaatga
     5581 agatgatgtt cgtaaaatac gaaaggtagt tggagaatct ataccctatc attcctcaga
     5641 tagaccgata ccagcatttt ctctacagca aaaaaagcag atttacaccg aacagttgtc
     5701 tgaaaaattt ttcaagactt atacgtacga accgaaaaag gaagaaattc ttcatgattt
     5761 gctgcaaaag ctgagcataa atgaagaaga aggatatata agagaaatgc gaaagcagat
     5821 ccagcaacgt gaaaatatgg gacaaatcat attcagtgat acagtggcag tcccacatcc
     5881 cgctgttcca gttggaatcg aaacaaaggt agccgttgca ttgattcctt ctgggatgca
     5941 gtgggatcaa tatcaagaaa ttcattttgt ttttttagtt tcgccttctt atgtcgaaaa
     6001 tgaaggaatt acggtcgtga ctaaagcaat cgttaggtta gttgaccagc taaaagtaca
     6061 gcaggaaatc ttagctgaac ctatttttga gaatttcagc agtaaattta tcaaactgat
     6121 cgaataagga ggaaaacaga tggctgaaaa aataaacagt gaaacactac aagtgacttc
     6181 ttttgatatc attttccata gcggaaatgc acggacactg atacatgaag catttcgctc
     6241 gatgcgtaaa gagatgtttg aagaagtaga agagaagttg cgacaagcaa atgaagagat
     6301 tcttcatgcg catcagtcac aaactcaact tctgaaagaa tatgccagcg ggacaaaaat
     6361 agaaatggaa attatcatgg tccatgcaca agatcatctg atgacgacga tgacattatt
     6421 ggaagttgca caggaaatga attatttgta tcaaaaaaat aaatagaaag gaaggatcat
     6481 tgtgcaggat acagtaaaaa ggaaaatcga attagaaaaa aagcaactta gtttgaaaaa
     6541 catgtatttc aatcgttatc taatcgtacg ttacctgact gcctttttct ttttcatgaa
     6601 catgcaatgg atgattctgc ttctatcagc taaaagttta ggaagtagtt taccgatgat
     6661 tctgatttta gctattttgc ctgcagtagg agaacaagta aagctttatc gaaaacacca
     6721 aacgaacgtt ccatggacaa agtggtattt tttgttccag agtgtcttta atttattagt
     6781 gattccagtg ttgtttacct caggatttac attcttgtat ccttttatgg caaataacaa
     6841 tggaggacag ttatttgttt tcattcttgt tgtaagcggt atttttgcca gtgcatttat
     6901 acagtatcgt ttaaagaaaa tttcactcaa ccaagaccaa cagtatatcc gtattaaaca
     6961 atatgaaaaa gcgttatatt taggaaagga gaataactaa tgtttgattt tttgcagaaa
     7021 tacctgatgg gacctatggg gaaaattgcg caacttaaaa tcgttcgggc tgtcatggca
     7081 gcggggatgg cttctattcc tttcacgatt gtaggctcga tgtttttggt tttaaatatt
     7141 ttgccattac cgttcccatt tttagaagga ttttttaatg caactttttt caaagttagt
     7201 gacttgtaca tgatcgtcaa tacaatgacg atggggattt tatctattta ctttgccatt
     7261 gtgtttgctt atgaattaac gtcaattgaa cgagatgaac aaaaattaaa tgttaatccg
     7321 ctcacggggg cattactatc tgtttttgca ttctttatgt gtatccctga attgatcatg
     7381 actgatggta aaatcagctt gattgcttct atcaatgaga cagaaactgt agtaaatggt
     7441 attaggatgg gctcttttgt cgaacgttta ggaacttcag gaatctttac agctattatt
     7501 atgtcgatta ttgcggtcga attgtattgt atgtgtgtca agagaaattg ggtcataaaa
     7561 atgccagatg ttgttccgcc aggagtttct cgttctttca ctgctctgat cccaacattt
     7621 gtcattgcgt ttgtggtgat gatcatcaat ggattattag ttgcactagg aacggatatc
     7681 ttcaaaatga ttgctatccc atttgggttt gttacgcagt tgacgaatac atggcttggt
     7741 gtcatggtga tttatttctt gatccatgca ttgtggatcg tagggatcca tggagcgaat
     7801 atcatcactt ctttcttgac cccgatcgta ttagctaata tggcagctaa cgcacaaggc
     7861 gcaaattatc cgttggcagg agaattcaac aattcttatg taaccgttgg tggatcaggt
     7921 gcgactttag gattgatcat ctttattgca ttcctcgcga aatctgatca gctgaaagtg
     7981 ttaggaaaag cctcacttgt accaggatta ttcaatatca atgaaccaat cattttcggg
     8041 atgccaatcg tctacaatcc ctatttagcg gttccgtttt tcttagctcc gatggcttct
     8101 gctacactcg cttattttgc cattcggctg gaaatcgtca aaccgatgct tgcacagatg
     8161 ccgtggcctt caccagtcgg gatcggagca tttgttggta gtggtggtga ttggaaagct
     8221 gctgtgttag cagtcatttg tgcaatcatc gcattcatta tctggttccc atttatcaaa
     8281 ttctatgata ataaattatt aaatgaagaa agagaaaagg cagcagaatt agctagtgaa
     8341 aagttaaaat gatttctaag aaaaaaggaa ctgtgacaaa aaatcacagt cccttttttt
     8401 cttagaaatg ggaaaccact agtatataaa taccattatt cttcaatcaa ccgcttcacg
     8461 attgcataat ccccttcata aggttcatct acaccattga ttttttgata gggatccacg
     8521 ccttttcccg caataacgac tgaatcttgc ggagcgcttt ctgctaaagc gcgtcgaatc
     8581 gcttcaggac gatccatttc ataatgaacg atcaaatctg gattagtaat tgcttcattg
     8641 atttcttccg cgattttttt cggatcttca aatgctgggt catctgccgt caaaaaggcg
     8701 atgtcagctg tttctgatag cacttttcca aaatcatgtc gacgggagat tgctttgttt
     8761 cctgggctgc ctaatacaac aattacacgg ccatctggat gttcattttt ggcaaatttc
     8821 agcaatgttt tcaagcttag ataattatgt gcatagtcca catagacatg cgctccattg
     8881 gattggatca attgatccat tcggcctggt acacgagctt cttttaatcc gtcttgtgca
     8941 tcttgactgg aggcacctgt tagtgctgcg gcaatgatcg cactggctgc attttcttgg
     9001 ttgaatcctc cagccagcaa aatatcataa gcagtatcgc agatagctaa gcgatcttca
     9061 tgagaagata aagtaaaacc gtgagtacct ttatctgaac ggatcacttg ataattgctt
     9121 tcttccgaac gaccataagt gatcgttgga atctggaata gatcggctgt ttccctaagt
     9181 aaatcaaaat agtcactttc atgatttaaa acaactgttt ttgaattcaa gatcaattgt
     9241 cgcttgcaat agaaatagtc atcaaatgtc ggatgttcga tcggactgat atggtcaggc
     9301 gagatattca agaagattcc gacatctaaa gtcaagccgt atacgcgttg tgttttgtaa
     9361 gcttgtgaag aaacttccat cactagatga gtcataccat tttcaactgc ttctgacatc
     9421 atacgatata aatctaatga ctcaggcgtt gtcaaatggg atttgaaata tgttttgcca
     9481 tctaacgttg tattcatagt agaaagcaat gctgtttttt tatttgttgt gtgatccaag
     9541 attgcttttg taaagtacgc agcagttgtt ttaccttttg ttccagtgaa tccgatcact
     9601 ttcaatttat tttgcggata gtcgtaaaaa gccatagcaa gaagcgccat tgctttacgg
     9661 atatccgtta cgatgattcc tactgctgaa ttttcgtatg gttgttctga tacataatat
     9721 tgaataccct gttcgattgc tgaatccaaa tattcttctt tgaaattcat tcctttacag
     9781 aaaaacaaag tagaagcatc agcttttctt gaatcataac ttaactgaag aaattcagct
     9841 ttttctggta catcaagatg ccagcctctt gttgaaacaa attcttttaa tagattttct
     9901 tttaatagta aatgacggat gtcttctaaa gagaagctca ttgattgatc cctcgcttta
     9961 ttcaatttct tggtttatta tagcacactc tttttaatat atcgtttgtt tccgctatga
    10021 caaaatccag ctttattcaa attttcttga cacaggggac aagcggatga gtattttcat
    10081 aaacaatgaa ctgactgtaa tagataggag aacgaatagt gtctccaaca atccaaaaac
    10141 ataataattc aaaaagacga taagactatt ggtaagtaaa agtccttgcg gcattggaag
    10201 ccgaaaaaat ttgtggaaaa taatcggcgg tatcgttata ccgccatttg aggccccgac
    10261 tgtgtacaat gtgtaaaccc caagactgaa caaaagactt cctgaaatca gtgaaaagaa
    10321 atgtgaggag atcatttcca cttttggaac aatctctaaa acaacaggaa aaagcagact
    10381 tcccgctaca gtcttaaaaa atactgattt tcctaatagg acagcggcta gaagcagcat
    10441 cataagattg atgctccaaa cagtaactga gcgttggata gaaaaggccg tttcaactaa
    10501 atgtccgatg gcgcccactc cggcggaagc tatatcgtga gggagcaaga accaattcaa
    10561 tccaactgtg ataaagaaaa gacctaaaaa tataacgggt agttttttta tttcttgtgt
    10621 cattgatatc tcctataaat gttagccata aaaggctgta tatttcttta ctttaccaaa
    10681 aaaacgaaac cgaattcaaa aaaacaattg ttcttataaa agttatataa aatcgattgc
    10741 cagtttctgt tttaccaatt aaaatgatag taatgttttg tagaaagttg gaatctgacc
    10801 gatggataat caaagaaaac cagcattaga tcaattaaca ccgcaagaaa aaatggcacg
    10861 aggctctgct tggatgaccg ccagtaatat catttcaaga ttgctaggcg ctgtctatat
    10921 cattccctgg tatgcttgga tgggcgaaaa tgctaaggct gccaatggat tattcaatat
    10981 gggatacaat atatatgcat tgtttctact gatctctaca gcaggaatac ctgctgcgat
    11041 tgcaaaacaa actgcacgct ataattcatt gaatgaatat ggcacttcac gacgattatt
    11101 tatccgtgca ttacagatga tgggcgggtt aggcctgttg tttgctttgt tcatgtacat
    11161 cgcctctcct tggctagctc atgcttctgg tggcggagaa gaactgattc caacgatgcg
    11221 ttctttgagt atcgctgtat tggtttttcc ttgtatgagt gtgatacgag ggtatttgca
    11281 aggcaatcaa gaaatgatgc cttatgcgct gtcgcagatt gtagaacagg tggcacgtat
    11341 tttttatatg ctattggcta cattcatcat catgaaagtc atggatggtg actatgtgac
    11401 ggcggtcacg caatcaactt tcgcggcatt tatcggtatg ctagcaagta ttcttgtcct
    11461 tttgtatttt ttgaagaaac atcaagcgta tacatcggcg tttattcact acagcgaaaa
    11521 caaaataaat attgcaacga aggaattgtt gttagatacc gttaaagaag cgattccgtt
    11581 tattattgtg ggttcagggg taacgatctt caaattagtc gatcagttca cttttatgaa
    11641 gatcatgtct gcatccaccg agtattccaa tgcacagtta ctggatcttt tttcgatttt
    11701 tagtgccaat ccagataagt tgacgatggt agttattgca ttggcaacat cgatcgcgtc
    11761 tactgggtta ccgttgatta cagaagctgt tacgttgaaa gatcgagtag gtctggcaaa
    11821 attgacaagc ggaaatttac agttgttttc attttttatg tttccggcga catttggtgt
    11881 tatgttactt gcctatccgc taaatacatt attttataca ccggattctc taggaagtaa
    11941 tgtactgatc caagcaagtt ttgtaggact cttcctagga ctatatatgc tagtttccaa
    12001 catgctgcaa ggaatgtttg aaaacaaggc ggcaattaat tatttggtta ttgggtttct
    12061 agtgaaactc cttcttcaat acccagccat tcgtttattt gaagtatacg gccctttatt
    12121 ggcgacaatg atcggatttg ctgtgtcttg taccttgatc ttaaagagaa tccatgaagt
    12181 agcccgcttt aaaccaagtt ttgtctggcg caggacattg ctgatcttta ttcttacctt
    12241 gattatgctt ttggcttcgt ggatcacaaa gatgattttt ggtgcttttt taaacgaaga
    12301 tagcaaattc cagtctctat tgttgattct tgtagtagca ggtatagggg ctttagtgta
    12361 tggttattta gctttgaaaa tccgcttggc agaaagacta ctaggaccag gaatagcgcg
    12421 tttacgacat aaattaaaaa taaaataagt gaatcagaag agctagattg atgctctttt
    12481 gaaatggggg aaacgcatgc gtttagataa gtttttagca gatatgggac ttggcagccg
    12541 caaagaagtc aaaggattaa tcaaaaaagg aacgatcgaa gtcaatggac aagtggtgaa
    12601 aagcgataaa gcgcaagtaa atgaattcga agatgcggtt acttatttag gagaaatcat
    12661 tacgtatcaa aaagattttt actacatttt gcataaacct gcaggagtca tttctgctac
    12721 tcaggataat catgatcaaa ccgtaatcga tttattggaa gaccaggatt atcgggaaga
    12781 tctttttcca gtcggccgat tagataaaga cacagagggc ctcttgattt taacgaatga
    12841 cggggtgttt gcccatcagt tactatcacc taaaaaacat gtggagaaag aatatctagc
    12901 tgaggttagc ggtataatga cagaagaaga tatccgttta tttgcagaag gactgattat
    12961 tgatggcgat gagcagacat tgccggcaaa actccaaatt gatcaagtag atcaagagac
    13021 agaaacttca attatccgtc tgattttgca tgaaggaaag tttcaccaag ttaagcgaat
    13081 ggtcaaagca gtaggaaaag aagtcttata tttaaaaaga atccgtatgg gaaaattttg
    13141 gctgcctaaa gatttgaata aagggcaata tcgacccatg accaaagaag agctagaaca
    13201 agtgagagaa aaataattga atggatagct aaaagaataa gcagagtagt tgtagcgcca
    13261 tctgaaaagc agacttgtcc gtctgacttt cggatggctt ttttgtgaaa tttcggctta
    13321 tttccgtaga agctacttct ggaacaccgt ttgttcggaa taagggactg tgacaagact
    13381 tttgttacag tccccattgt tccttgtgac tcagagttag atagtaagga cacgatctcc
    13441 ctaatactca ggccaaaacg ctgaattcac aacctctgat aaacggtgtt caatcagctg
    13501 accctcggta ttagctcaaa aaacggcaaa atatgaggaa ctattttccc gtttttttct
    13561 ctaatactca ggccaaaacg ctgatttcac aacctctcct atactttgtt tcattttccc
    13621 tatactttgt ttatttattc tcattattga aagcgctata attacgttgc gaatgaatcg
    13681 aaaggggttt ttagatgaaa aaacgaaagg ggttcaatca tttttttatg aatctatggc
    13741 gttataaagc acttgtcctg atggctgtac cgggtatgct ttggatgatt tttttctttt
    13801 acatacctgt aatggcgaat gtcgtagcat tcaaaaattt tcatatttca gcagatgggt
    13861 tcttggctag cttgagggaa agtccttggg tgggttggga aaatttcaaa tttttatttt
    13921 cttcggatca agcatttctc atcactaaaa atacaatttt atataatgta gcatttatcc
    13981 ttttgaatct gctgatctca gtcgtatttg caatcattat gagcgagttg agaaataaac
    14041 gactcgttaa agtttatcaa acgatgtcct tactacctta ttttctatct tgggtcatta
    14101 tcagttactt tgtttacgct tttttaagtc ctgacaaagg aatattcaat cagtggatca
    14161 ctggacatgg aggagaagct gtcaattggt ataatgaacc gaaatattgg ccgtttatct
    14221 tggtgttcat tggtacatgg aaagggattg gttataacag cattatttac tttgcatctg
    14281 ttatgggaat cgatccgact tattatgaag ctgctatggt tgatggagct agcaaatggc
    14341 agcagatcaa acatgtgacg ataccacaac ttattccact aatgacgatt ttaacgattt
    14401 tagccgtagg aaacattttc cgagctgatt ttggcctgtt ttataacatt cctaggaatt
    14461 ccggtgcatt atatgatgtg actcaagttt tggatacgta tatctacaat ggcctgacat
    14521 ccacaggaga ttttggtatg acggctgctg caggacttta ccaatcagtt gtcggctttg
    14581 tcttgttgat gatcacaaat acgatcgccc gacgttttga tagcgattct gcattattct
    14641 aaggaggaaa aaaatgaaaa aaaagaaagt ggagagagtc caagtccgct ctttcaataa
    14701 gacggctaat tttttctcta atataatgat tgcactcttt gctatttcct gtattttgcc
    14761 atttatattt gttatcgcca tctcttttac cgatgagtcc atattgtcga ctcacgggta
    14821 cagtttttgg ccagaaaagt tcagtatatt tggttatacg tacttgttcg accagatgca
    14881 ggataaaatt ttccaagcgc ttttcgtcac tgtacttgtt actgttttag gtacactgat
    14941 caatagcacg gctacttctt tatatgctta tgcgatttca cggtcaaact ttccattccg
    15001 tcgttttttt accgtttttt gtttgattac gatgttgttc tcaccaggga tggtggccaa
    15061 ttatcttgtt atgacaaatc ttcttcagtt aaaagatacg atttgggcat tgatcttgcc
    15121 aatggctgtc agtcccttca atattattgt gatgcgcaca ttctttaaac gttcggtctc
    15181 tgattcgatt attgaatcgg cacggatcga tggagctagc gaactgcgaa tatttgttca
    15241 gatcgtttta ccgttagctg ttccaggtat tgcgacaatc agtttatttg ccgcattggg
    15301 ttactggaat gactggttca atgctttact atacatccaa gatgataagc tagttccact
    15361 acagtatctt ttgatgaaaa tccaaagtaa tattcaatat ctgacacaaa attcaggagc
    15421 aggaagccaa ttgacaggcg gattagcctc agttccagga gaatctgcca gaatggcgat
    15481 cgtagtcatc tcgacgttgc ctattgcgat cagctatcca tttttccaga agcattttgt
    15541 caaaggtcta acaatcggcg gagtaaaaga ataaagaaac aaaaatataa atcataaaat
    15601 taggaggaag aaaaatgaag ttatggaaga aactagcctt tacaggtgtt tcagcgttgt
    15661 tgttgggaac attagcagca tgcggaggtt ctggttctaa agaccaggca gaagctagca
    15721 attcggacac tttgcagatg tatcaaatcg gagataaacc agataatttc gatcaattga
    15781 tggaagtagc aaacaaacga atcgaaaaag agatcggtgt aaaagtcaat atcaattaca
    15841 ttggctgggg cgattacgaa aagaaaatga acgtcatcat ttcttccggg gaaaattatg
    15901 atatcgcctt tgcgaacaac tatgtttcca atgcccaaaa aggagccttt gctgatttga
    15961 ctgaattagc gcctaagtac gctgaaaaag cttataatga tttagatgaa gcatatatta
    16021 aaggaaatct ggtcaatgga aaattatatg ctttcccagt caatggaaat gtctttgctc
    16081 aacaagtact tacttttaac aaagctttac tagataaata cgatctttca attgatggaa
    16141 tccaatctta cgctgatgca gaaaaagtct tgaaggaatt ccatgaaaaa gaaccaaaca
    16201 cggcagcttt tgccattgga caaggcttca aagtccaagg gaactttgat tatcctttag
    16261 ggaatacgtt accatttgct attgatctgg atggagatga ttcgaaaatc atcaaccaat
    16321 acgataatga aacgtatatc ggactgttac gtacaatgca tagctggtac aaacaaggac
    16381 tcattccaag tgacgctgca acgagcaata aggattatcc actagaagga aatacttggc
    16441 taatgcgtgg agaaacacaa ggaccgtatg attatggcga tacaattttg acaaatgcag
    16501 caaaacaaga acttgtctca aaagcaatta ctgttccact aaaatctaca gctcaagcac
    16561 aaatggcaaa ttttgtcgta tcaaatactt caaaaaataa agaaaaatca gtagaacttc
    16621 tagggctgct taacagtgat ccagaattat tgaatgggct tgtttggggg atcgagggag
    16681 aagcatggga aaaagtcgat ggttcagatg ataaaatcaa attgctagat ggttatcagc
    16741 caaatacaca tatgtctgct tggaatacag gaaacaacaa gattctatat acacaagagt
    16801 ctattacgga tgacatgatt gccaaacgtg atcaatcgat tgctgatgca gaaacttcac
    16861 cgattttagg attcagcttc aacactgaca gtgtcaaaac agaattaagt aatatttcta
    16921 atgtcatgaa tcaatattta gatggattga atacagggac tgttgatcca gatgaaacat
    16981 taccgaaatt gaaagacgct ttggacaagg ctggctatga taaggtattg aaagaaatgc
    17041 aaaaacaata tgatgagttc cgacaagaat aataatgaat aaagacaaag gggttgtgac
    17101 aaaagacgtg tcacaacctc ttgttccgaa taaacggtgt tcaaaagcca gtttctcggt
    17161 attaagctaa atatatgtaa aaatgtggaa agccatattc acaaaatttt tcttactact
    17221 cgaaactaag cggctatttc acaaactctt ggtaaacggt gttcaatcag ctaaccctcg
    17281 gtattcgctc acaaaacgac aaaatatgag gaactatttt tccgtttgtt tctctaataa
    17341 tcaggtcaag gtgctgattg cccaaccttt ttttgtataa tgagaaagta taaacttaat
    17401 gtttggagga gaagatatgt tagggaaggt tttggaagag ttatttatcc ggatctgggt
    17461 cattataaaa ctaaccttgt atttctggat atatacattt gccggcggga tcatctttgg
    17521 attaggtgcg gcatggaaaa cagtcaatga actattttat ttgtacggat tcgagtataa
    17581 agaaatcaca ataaaaagag gctggaatat atataaaaga aattttttaa gaggcaatct
    17641 gctatttagt ttatttttat ctggtactgc tttattgagt tacaatttat atttatctgt
    17701 tcaaatcaaa gggctcattt ttttagctat tgattttatt ttactttttg ccttgctctg
    17761 cctatttgct acttatcaat acagtatgat tttagatagt gaatatacga tctctgttcc
    17821 taatctgcta aagctaagtt ttatttcggt tttttccagc ttttcgtctt ttctaaaaat
    17881 aattattggt agcggtataa ttttaggcgt gacttggcaa ttcaaaggat tgatcctgtt
    17941 tggtgtcatt ggtcttttga cggtttggaa tggtacgatg acaacacatt ggcgtgaaga
    18001 attagataaa cagttggaaa gttatgaata agtttcggat tctattttca aaaggaacct
    18061 tactaaatcg tctgttgcgg aaatacgcgg ttatcatgat cggtgtaacg atgactgcta
    18121 ccgttatttt tagtatccat acttgggaac aaaatcaaaa acaggcagaa aacatgacaa
    18181 gtgatgccgt tcaaagcaca agcagaatgc tgaatgataa aacaaccttg agtaggatca
    18241 tcaaaaacca gcttgtaggg aattccgaaa agatcgaaaa tgtgacgacc tacttaacaa
    18301 aacccataga tcagtatctg atgtatgtat acgaacagca aaacagtacc gatgaattag
    18361 tttctttccc aaatcaaatc aaagatctct acgcaaatta tgaagaacta agtgcgatat
    18421 atatcgtgtt gaatcaacta ccggagtatt atgaatcttc tagagaaaac aaaggtgggg
    18481 aaataaaaaa ggggacacca aaattactag acgcctttta tatcaagttg cctatcacac
    18541 aaggaggagc tgagtcagga agtattttta ttggttttca aaagaaagaa ctagatatga
    18601 ttttagaaaa tctaacgtca ttcaatggac tctctttata catgatttct ggaacgacaa
    18661 atcgactata cacattccat gataataaaa tagctaaaga aacgtttgaa agacaagaaa
    18721 tgatcatcac agagagtctg aagaaaaatt cacaattgcc gattgacgat ctggaaaaaa
    18781 acaattttgt tcaacaccaa gaactgtctg atgattatcg tatattagct gtactagata
    18841 aaaaaagtgt aaaaatcgaa acgggaaaaa atcttgcccc gctgatcatt ggtatcatta
    18901 ttttagacaa tatactgctt atctttcttt ataaaacatt caaacgatat tcacagcaag
    18961 tatctatcgt catgcaggcc atggatcaaa cagcagcagg gaatttagag acgcggatcg
    19021 acataacaaa cacggaatat gaactgaggg aactttcaat cggtatcaac gaaatgttgg
    19081 atagcatcaa tcagtttgta gaagacatct acaaattaga aatcaaacag caagacgcac
    19141 atatgcgggc gcttcaagca cagatcaatc cacacttttt atataataca ttagaatata
    19201 tccgaatgta tgcaatcagc gaaggaagtg aagaattagc agatgttgtg tatgcgtttt
    19261 ccgctttgct tcgaaacaat acgaatcaag agaaaacgat cactttaaaa gaagaactgg
    19321 acttttgcga aaaatatgtc tatttgtatc aaatgcgtta tcctaatcga gtagcctatc
    19381 attttatgat cgatcctgat ttggaaaaga tcgaagtacc taaatttgtg atccagccat
    19441 tagttgaaaa ttattttaaa cacggtattg attttactcg attcgataat gcgctaagtg
    19501 tcaaagtctt gcaggaaggc aaacgggtaa ggatcatcat caaagataat ggaaaaggga
    19561 tgactgaaaa acggctaaaa cagattgaag aaaaactgtc tcatcctaaa gtggaacttc
    19621 acggttctat cggtctgcaa aacgtgaatg aacgtttacg tgccagcttc ggcagcagtt
    19681 attatatgtc gcttgagaat aatgagacag gtggactgac cgtttctatt acattcaagg
    19741 agggatgagc gtgtatcaag tgttattggt agatgatgaa tacatgattt tgaatgggtt
    19801 aaaaaagata attgattggc aaagtttagg ctttcaaata gtagcaacag cagagaatgc
    19861 caaagaagga ctagctgtac ttgaacagcg acagattgat ttagtagtaa cggatgtgac
    19921 aatgccagaa ataaatggac tggaatttat tgaagcggct caaaaagaac gtcataattt
    19981 tgaatttatg attttatcag gataccaaga attcgattat ttgaaaggag gtatgcaatt
    20041 aggcgccgtg aattatttga tgaaacccgt gaataagttc gaattggttg aaagcctcaa
    20101 gaaaatcaaa acacggttag atcagcaaaa tgaacaaaaa aatcaacaag aaatctatca
    20161 agaactgtta ttcagtcaat ggttgaatga agaattggat gaaataagtg aagaagaaat
    20221 aataggacaa ataagtgaga agcagcatag gatagtattg attcaactgg caagaactca
    20281 tgatactctt atcaatagat ggttgcaaga acggcaacaa atcttttact atcaacgaaa
    20341 ttacggagac cgtgtattga tcacgttgtt gttggaggca gatgaagttg atggcttctg
    20401 ttgttttgtt caagaaaact ttcctgatca ggaatggctg atcagtatcg gggaagagac
    20461 agattctttg gacaaaatcc ctgaaacatt ccaacaagcg aaagacaatc tacagttgca
    20521 tcaattttat ggagataaag acgagcatgt tttttacgca gtacaagcag aaacaaaaga
    20581 tcaaaccatc gatttttcaa gtttcagacg cgtgctgcaa aataaacaat tggaagtcgc
    20641 tcaaaaaatg atcactgatt tttttgaaca gtttcagtta gcagccatga tgccggaaga
    20701 tatacgatat tccagttttt tactgtttat ggagattcaa cgggaattga ttgatctgga
    20761 agatgaggaa tatttacaag gaatcgaaaa aatccatcag gcaaaaacgg tgcaagaact
    20821 tcagcagcta cttctgtcat tcatcaaaaa gcatcagaga cagaaaaagt acagtgacaa
    20881 tgtggaaaaa gtaatcgtaa tcctgcacca gcattatcaa gaaccactta cactcaaaga
    20941 agtgtcagaa aacctgcatt taaatgtcat gtatctagga cagctgttca aaaaagaaac
    21001 gaaaaaaagt ttttcagctt atttgaacca tttacgtatg gaaaaagcga aacaactgct
    21061 tttgcacagt aatcaaaaca tcaatgagat agcaagtgag attggctaca acaacaccac
    21121 gtatttctct aaattattca agaaaatcgt cggcagatcc cctaaagagt accgagaaaa
    21181 cagtggagaa tcataaaaga aaaagccaat gtaatgaaat aacttataaa aaattgtcta
    21241 gaaatctaat tttgggctgt tcttattact tgaggcaagg cagctttttc atgacctctc
    21301 gataaacgga gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtgg
    21361 aaagccattt tcacaaaatt tttcttaata ctcgaaacta aacagctttt tcacgacctc
    21421 tccactaaac ggtgttcaat cagctgaccc tcggtattag ctcaaaaaat ggcaaaatat
    21481 gaggaactat tttgccattt gtctctctaa tactcaggtc agaacgctga tttcacaacc
    21541 tctcaataaa cggtgttcaa aagcttgcat ctgcggtaat aagaccaacc aaacgaaaaa
    21601 tatgaggatc tatttttgct tggctgttct tattacttga tgcaggacag ctttttcaca
    21661 acctcttgtt aattatagtt tgataaggaa aaaggcaaat cttttgcttg atatttctta
    21721 gggtgaggga tgatccctaa ttggtaagag atcgtgccgc ctttcagcaa atcttgatgg
    21781 gtaaaatata atttcgtata ttctctgtcc atccgtttga ttttatggat aaattgttgc
    21841 tgcggccgat taggcgttgc ttcaatcgtc aattcctttc catttgacaa aagcaaaaca
    21901 gccttgtcaa ttaatggcat accgataaca tattcaccgg ttccaggcgt gacaggataa
    21961 aatcccagac tattgaagat ataccaagcg gccatacttc cattgtcctc atctccaggg
    22021 taacccattg gagaatcgtc gaaggtctga gtcatcaact gtttaagcag tggctgtgcc
    22081 atttcaggtt gtcctatata actgaacaag taaggatagt gaaaacttgg ctggtttgaa
    22141 atagctaatt gtccaaattc tatcgcggcc atctcactca tttcgtggat ctcaaaacca
    22201 tatcctcctg ggtcaaacgt tgggggctga ttgcacagct caatgagttt ttctttatat
    22261 ctctcactgc caccatattt tccgatcagt cctgaaaaat cgtgataaac agcaaagctg
    22321 ctttgccaag cacttccttc tgcataatct tgtccccaac gattagctga aaatggttct
    22381 ctgaacttgc catctttatc ttttgaacgc ataaatcctg actcatagtc aaagatattt
    22441 tgatagttca atgattgctg ctgatatttc ttcgtcattt cctcttcttc tagtaccttg
    22501 gcaacttggc taatacaaaa atcgctataa gcatagtcta atgtgtgatt gactgattcg
    22561 tggtagtgat taggaacata gccatatgtc aagtaatctt ctgttccttg gcgtccataa
    22621 tttggatgtt tgctttgaac cgtagcacct ttttccattg ccgttagaaa ttcaggcatc
    22681 aagtctcgtc ggatatcttt tacagcagca tctgcaatga ccgcatcgat caatgtccca
    22741 ggcataagtc cacgttcatc aggagacagc cattttggca aatagccact ttctctatag
    22801 ctgttcaaaa aaccttccag catttcttcg tatttttcta cagcaatcaa agaataaaga
    22861 ggataaaccg ttttatacgt atcccaaaac ccattatttg tatagagaac accaggtttt
    22921 atagatcgac tagtcgtatc atagtggatt ttttgcccac taggatcaat ttcataaaat
    22981 gtttgtggaa ataaaaacac ccgatataaa ttatgataaa acgtccgcaa ttgttgttga
    23041 tcatggtgtg tgagctgtat acgatcaaaa taggactgcc attgttcttg actgtgagca
    23101 aggtaagtct ctttgctttg gttttgttct agttccaggt tataagctgc ttgttccgag
    23161 ctgataaaag atgtcccaaa tcggatagtt tgtttttttg tctgttcaaa atacaacggg
    23221 atcgagtcgc tatttttgtt ggaaacagct aactcatcaa ttgttaaagg atattcaaat
    23281 ctcaatataa aataaaaaga aaaatcagga tcttctgaac cagcaaaatg tgtgattttt
    23341 ccttccactg tgtaaggatc tataacaaag agctgatggc gtcccggaaa ggataaaagt
    23401 aaaccgctgt catttttgct gtaatcaata gataagattc cgccatacat gctaggtatc
    23461 aatgtagaac agatgccata acgtaattga cagatcgaca gatgagtcgg gcaaaatata
    23521 ctttcttctg ggcggtaaga actttgagca tggaaaaggg tgttctcctg tagttttcca
    23581 ttgatcggcg taaacagcat atgactgaaa tcacccatcc aaggacttgg ttgatgggta
    23641 agacggtagc cttgaaaaac gcggtcatca ggatgaaacc accagctgcc gttttgatcc
    23701 gtagtttgcg gtgcaaaata attcatccca aaaggaacgc cagtataagg ctggcagttc
    23761 ccattagaga aatttggctg attcgctgtt gcatgtctag tatctatttg accgatattc
    23821 attttatttc tcctttgttg attgtctttt gatgaatgaa actggaaatt tactatcgga
    23881 tggtcgatct tttgtctcta accaagtgac caaagcatct cccgcaagct tacctaactg
    23941 ttcaaaatct tgtgcgatgg ttgtcagtgg cgggtcaaca agagctgctg cttggatatc
    24001 atcaaatccg ataattgaaa aatcttcagg gatatgataa ccatgctgtc tcaagagatt
    24061 catggtctgg atagcgacaa catcgttttc acaaacaaaa gcagttactt catacttagt
    24121 gatataagaa agcaattcat ctaatgtcgc gcttttgtca tccagtgaag tgtggaaagt
    24181 caaaccagct ttgtccaatg cttcaaggta accaaggtaa cgctgtcgaa cggattgtgg
    24241 atgagtctgc gtaccaaaca aataagcgat ttttgaatgt ccctgttcga tcaaataaga
    24301 ggcagcaagc gcaccgcctt ggaaattatc ggaaagaacg gtaggaaaag gtaactcata
    24361 gattttttta tctaatatga cgacagggaa ttctttcaat gaaagctcaa aaagtaagtc
    24421 aagatgttgt tctgtatcaa gagcataata aatcaatccg tcaaattcat tgattagctc
    24481 ttttgctgtt ttattttgta gaaagtcaag cgtcgtcatc atgacatcaa tctgtttatc
    24541 ttgcatcaca gggttcaatc ccttgctaaa atcaccaaca gacaaatcag cgacatatgg
    24601 aagaagaaac agaatccgtc gtgtttttgt aggagaagca agaatctgtt ccggagaagc
    24661 ggatttaacg aaactgcctt tcccgcgaac gcgataaata agtcctaatt gctctaattc
    24721 agtcaatgca cgtttggaag tgattctgct tactttatag gtacgtgaaa gttctttttc
    24781 agttggtagt tggctgtcta caggtaattc accagttaaa atagccgttt gcaaatcatt
    24841 aaatattgtc tggtacaatg gttgagtcat ataaacacct cttaatgata tatcatattc
    24901 aaataatacc ataatggatg aaaaagtaaa taaaaagata ttttttgaaa gcgtagacat
    24961 aattggctgt atgaagtata atctttaaag atatatcaaa tagaaagaag gttttattag
    25021 tggtttataa agatatacca aaatcggtgc agaaatttat ggaagatatc acacaaaaat
    25081 gtggggatat ccatgcggac tgggcaaaga attttacagc agcatttgcc aatactctgt
    25141 tgacaacagt caaaaagcaa aatgacggaa ccaccttttt attgacggga gacattccag
    25201 cgatgtggtt aagagactct actgcacaag ttcgtcctta tttagtgatt gcaaaagaag
    25261 atgaggaatt agctgacatg atcagcgggt tagtcaaaaa gcaatttttt tatatcaata
    25321 tcgatccata tgcgaatgcc ttcaacgaag aagctaacgg tgctggccac caaacagatc
    25381 atacaaaaat gaacgattgg atctgggaaa gaaaatatga aattgattct ttatgctacc
    25441 caatacagtt ggcctatctt ctttacaaaa atacaggaaa aactgatcaa ttcaatcaat
    25501 cttttattga aggtatcaaa aaaatcttgg acgtgttcaa aacagagcaa gatcatgaac
    25561 actctcctta tacatttgta cgagacacga caagaaaaga agatacgctg atcaatgacg
    25621 ggcgaggtgc aaaaacagct tttacaggaa tgacatggtc tggtttccgt cctagcgatg
    25681 atgcttgccg ttatggatat ctggtaccat ccaatatgtt tgcggtggtc gtgttaggtt
    25741 atgtggaaga aatttttcaa acgatactcg atgataaaga aattgtacag caaacacaga
    25801 aattgaagaa agagatccaa gaggggattc agaatcacgg acgaacggtt aatcaaaaag
    25861 ggcaaacgat ttatgcatat gaagtagatg gaatgggaaa cgccacagtc atggatgaca
    25921 gtaatgtgcc caatctgatc gccgcacctt acttgggata ttgttctgaa caagaggaag
    25981 attatctgca aacaagaaga acattgttga gtaaagaaaa tccatatttc tatgaaggaa
    26041 agtacgcaaa aggaattgga tcctcccata cgccagaaaa atacgtttgg ccgattgcat
    26101 tagctatgga aggaatgaca acgtcagaca aatctgaaaa agagcggata ttgaatttac
    26161 ttgtagcaac tgacgcagga acacacctta tgcacgaagg attcgatgtg aacaatcctg
    26221 aaaattatac gagagaatgg ttttcttggg caaatatgat gttttgcgaa ttagtcatgg
    26281 attactttga tattagagta gaaaagtagg aggtatcgta aatgaaaaag aaagtatata
    26341 tcatttctca tagccactgg gatcgtgaat ggtacatggc ttacgaacag catcacatgc
    26401 gactgattga attgatagac gatctactag agctatttga agttgatccg tccttcaaca
    26461 gttttcattt ggatgggcaa acgattattt tagatgacta tcttcaagta aggccagaaa
    26521 aaagacaggc aatccaacaa gcaatcaatg aaggaaaatt gagaattgga ccattttata
    26581 tcttgcaaga tgattttctt atcagctcgg aatcgaacgt gagaaatatg ctgattggca
    26641 tggaagaaag ccgtaaatgg ggaacccctg tcatgttagg ctattttccc gatacatttg
    26701 gaaatatggg acagacgcct caattaatga aacaagctgg tatttcagca gcagcgtttg
    26761 gccgtggtgt aaaaccaatt ggctttgata atcaagtttt ggaagcagaa aactattctt
    26821 cccaatactc agagatgtgg tggaaaggcc ctgatcaaac agcaatcttt ggtctgctat
    26881 ttgccaattg gtatagcaac gggaatgaga ttccagtaga aaaagaagca gctcttgcct
    26941 tttggaagca aaaattagcg gatgcagaac aatatgcctc tacgaaccac ttattgatga
    27001 tgaatggtgt tgatcatcaa ccggtacaaa aagatatatc aaaagcaatc catctcgcaa
    27061 atgaactgtt tccagattac gagtttattc attctaattt tactgactat ctggaagcag
    27121 ttcaaaaaga tgtgccagaa gacttaggct cagtagaagg agaattgaca agtcaagaaa
    27181 cggatggctg gtatacttta gccaatacag catctgctcg ggtttactta aaacaatgga
    27241 ataccaaggt ccagcgacag ctagaaaata ttacagaacc acttgcgacg atggcttatg
    27301 aagttagtgg aaactatccc catgatcaat tagattatgc ttggaagaca ttaatgcaaa
    27361 atcatcctca cgacagtatt tgcggttgtt cagtcgattc tgtccatcgg gaaatgatac
    27421 ctcgttttga aaaagcagat gaagttggaa agtatcttgc acaagattcc ttagaacagc
    27481 tgaccgcagc aatcgatacg accggattcc caaaagacag ctttccattt gtcattgtaa
    27541 atactgcagg aatggataaa actggtgaag cagaaataac catcgaatta gaaagaaaaa
    27601 gattcgctga aggaatacct gaacaactat atcaagagtt agaaaatttg cctaaacgta
    27661 agtatcatgt agaaacaaaa agtggggcca ctataccagc tattttatct gaagaaactg
    27721 tccaatttgg ttatgatttg ccgaaagacc gtttccgtgt gccatatatg gcaagaatga
    27781 tcaaagtgac tttaccatta gaaaacatgc cagcattttc ttgggaaaca ttcgcgcttg
    27841 tagaagggga agcgaaagca gaagaaaagg aaacaatgat tcatcagagt ggacgaatca
    27901 tcgaaaacgg ccctctacat ttgacgattg aaaaaaatgg taccattaca atggaagatc
    27961 gcaaaaacaa acgaaagttg aataatctgc atatttttga agatatcggc gatataggaa
    28021 atgagtatat ctttaaacaa cctttctgtg atcagccaat cctatccagt aacaaagaaa
    28081 actctgaagt aaaagtatta gtagatacac cggaaatagc aaaaataagt attctacagg
    28141 aaatggaaat ccctgtttct gcagacgagc gtttagaaaa agaacagcag atggttgtgg
    28201 aatttcgtta tcgaaaagca gaacgatcca aagaaaaacg aatccttcaa ataaaaacaa
    28261 tcatgacaat cagaaaagat agtaaaaaaa tcgactttga aacgactctc gacaatcaga
    28321 tgaaagatca tcgcttacgt gtcttgtttc ctacaaagtt acatgtggaa catcacgaag
    28381 cagatagtat ttttgaagtg gtgaagcgcc caaaccatgt gtctaaaagt tgggaaaacc
    28441 ccacgaatcc gcaacatcag caagcatttg tcaatattca tgatgaagag tacggtgtaa
    28501 cagtcggaaa ctttggtttg aatgaatatg aagtaacaga agacggacaa atcgcagtga
    28561 cgctacttcg cagtgtggga gaactaggcg attggggata ttttccaaca ccagaagcac
    28621 agtgtctagg tgagcatcgg ttcaattaca gcattgaact acatggacca gaagaaaaat
    28681 tctctactta tttgcatgca tacgcagcac agattccttt ttccactcaa caaatcaaac
    28741 atcatgaagg aacattgatt tcaaaacaac aatatttaac gataaaaagt gaaacgtttg
    28801 caattacagc tttgaaacgc agcaagttta gtgataaagt agtagtaaga ggtttcaata
    28861 tgtctagtca tttagaaaaa ttggaaataa ctaaagacaa tggaaaaact gtcatattga
    28921 atctgcttga agaaccaacg aagcaagcgg ttgtcccaat aatccaacct tatgagattc
    28981 ggacaatcgg attcgaggag gaaaacgaat gtatggcgga atagaagcag gcggaacaaa
    29041 atttgtgtgt gcggtttcag atggtggaga aattttagaa aaattaagta tccctaccac
    29101 aacaccagaa gaaacattgg atcaggtatt tgaatttttt gatcgttatg aattgaaggg
    29161 aatcgggatt ggctcattcg gcccgattgg tatcgacaaa acaaaagata attacggata
    29221 cgttttggca actcccaaaa aagggtgggc acaattcgat tttcttggca caatcaagga
    29281 acgctacaat attcctgctg cttggacaac ggatgtcaat gctgctgcat atggggaatt
    29341 gaagcaagga gcagcacaag gaaaaaatag ttgtatttac cttaccgtgg gaacaggaat
    29401 cggtgcaggt gtcgtgatca atgaagaaat tttttcaggg atcgcacatc ctgaaatggg
    29461 acatatttgg gtgaaacgcc atccagatga ccattatgaa ggtacttgtc cttatcataa
    29521 agattgttta gaaggattag cagcaggacc gtctattgag gcacgcacag ggatcaaagg
    29581 gcaagatctg ccgcaagatc atcctgtttg ggctattcag gcttattata ttgctcaagc
    29641 actgatcaac tatacgttga cgttagcacc tgaaaagatt attttaggcg gtggggtaat
    29701 gaatcaggat catctccttc aaaaaatccg tcagcaattt gttgaattga tgggtggtta
    29761 tatggaaaca cctcaagtaa gtgaatatat cgttcgttgg ggactgccga atgaaagtgg
    29821 gattatcggt agtttgctgt tagcagaaca cgaacatcaa aagtaagcag acaaattaca
    29881 caaaaagaac agagtcttat cattatctgc tgataagact ctgttctttt ttatttctat
    29941 tcccataatc caggtaaagc tttaccgact attttttctt gagtaaatgg atggatcatg
    30001 tgcagttcta atgcatggag catcagtcgt tcagctgttc tattttggta gagagggtct
    30061 ccaacgatcg gatgtccctt atgagctaaa tgaactctga tctggtgtgt acgtccggtt
    30121 tccaaaacac aatagacttg tgtttgttca gaaagtactt tgttgacagt gacatgtgtc
    30181 aaggcgctct gaccattttt atcatctacg accctttttc tccgatcatg gcgatctcgt
    30241 ccaattttat tggtgatggt catatctttg gcaatttctc cccatacgat ggcttgatat
    30301 ctccgataga tcattttttg ttccaacatt cttccaagta tcggcaagac aaatggattt
    30361 tttgcaaata aaacggcacc gcttgtttct ttgtccaagc ggtggacaac gtaaggtcgt
    30421 tgctgtttag tgtcgagata agcagctaaa tgattgagta aggtactgtt ttctgtcggt
    30481 tcatttggat gagtcttcat tcctgcaggc ttatccacta taatcaaatg ctcatcttca
    30541 tataaaacac ggatgtgctt cgcatctcct aattgaactt cttgataatt ataatctgtt
    30601 tcttcgaata acaatgtgat ctggtcattg ccctttactt catgatggaa caaagcaggt
    30661 tgttgattga tccaaacgtt tttacgtata cgcaaaaagt gtcgaacctt tctaggaact
    30721 aaccattcct tttcaagcaa gtcgcgaatt gttgtttcag gatggttcat aggtaaagtg
    30781 atcgtgattt tcacaagtgt actcctttca gtgcaattgc agactaattg taccattttt
    30841 aaaacatatt taagaaaaaa cttacatttt tctgttcatt cttatgttac tctacttgca
    30901 ctaacgtaaa aaaacatatt gaactacaac ttaagcagga agaaaacact ctttcactat
    30961 ggtaaaataa gacgattgaa tcaaatagac aggagaagaa gcaaatggat tttcaaagca
    31021 ttttagaaaa gataaaagct gctttgatca gtttttggcg ttggatcaga ccatatctcg
    31081 gacaattcca tcgatggcga aagcgcatct ggaaaaaata tcatgtgaac aaattgattt
    31141 tgctgattgg tttgatctgt gtcttagtga caagtatcta cctctttatt ttagcaaaac
    31201 aagcaaatgt cgaaacgtta aaatcaggat taagccaatc gacagtcatt tatgataaga
    31261 aaaacgaaga agctggcaca ctgtacgccc aaaaaggaac atatgtagaa ttaaacgcga
    31321 tttctcccta tatccaagat gcagtaatct cgacagaaga taggaatttt tacgagcatc
    31381 acgggtttga tatcaaaggg attgcccgtg cagcagtccg aatggtcttg tctggcggaa
    31441 catcaggggg cggaggaagt acgatcactc agcagcttgc caaaaatgcg tatctgacat
    31501 tagaccaaac gtttgatcga aaagcgaagg aattattttt ggcaatcgag atcgaaaaga
    31561 agtacagcaa agaagaaatc ttgaccatgt atcttaacaa tgcctatttt ggcaacgggg
    31621 tatggggtgt ccaagacgct tccagaaagt attttggagt agatgcaacg aatgttacgc
    31681 tgtctgaggc tgctactttg gcaggaatgc tgaaaggtcc tggtatttat aatccgatcg
    31741 atcacccaaa aaatgcgaat gatcggaaaa acacagtact aagtgtaatg gaagaaaacg
    31801 gaaaaatcac aaaagaacaa gaacaagcag aaagccagac agatatcagc gcgtatctta
    31861 atgacacata cgataattca gattctggtt atcgttatcc ttattatttt gatgcagtga
    31921 tcgatgaagc agttaacgag tatggtcttg atgaagaaga tgtcatgaat aaaggctata
    31981 aaatctatac agctttagat caggattatc aaaagcaatt agaagctact tatcaaaaca
    32041 gtgcattatt cccagcaaat gcttcagatg gtgccatggt ccaatctgct tcagtagctt
    32101 tagatccgaa tacaggtggt gtacaagcac tagtaggacg aagaggagat catgtcttta
    32161 gaggattcag ctttgctaca cagatgcgtc gttcaccagg atcaacgatc aaaccattaa
    32221 gcgtatatac accagcactg gaagctggat acaagccttc cagtatcttg aaagacgaac
    32281 cgcaaagtta ttacgatgcg cataattttg acgggacata ccaaggagaa gtaccgatgt
    32341 atcaagctgt tgcacaaagt ttgaatttgc ctgctgtatg gcttttgcat gagatcggtt
    32401 tacaaaaagg atatgataaa gcagaagaat tcggcctgcc tttagcaaaa tctgataagt
    32461 attatggctt agcccttggt ggactggaaa aaggagtatc tccattgatc atggcgggtg
    32521 cttacagtgc atttgccaat gatggtcaga tgtatacgcc gcatcttatc acgaaaatcg
    32581 ttgattcgac tggagcggtg atcgtcgata acacaaacaa aaaaccaaaa caggtcatat
    32641 caaaggaaac agccgatgaa atgaccagca tgcttcttgg tacattttca aatggaacag
    32701 cggctgctgc gaatcctgca ggttatacga tagcagggaa aacaggaacc acagaaacta
    32761 atttcgatgc cacaaaagca aatgaccaat ggatgatcgg ttacacgcca gacgtagtga
    32821 tttcgacatg gatgggttac gaagaatcca gcaagctgca ctatttggaa gggacaagtg
    32881 gaaatgttgt cgggaaagtc ttcaaatcag cggcagaagg catccttccg tatacttcaa
    32941 aaacaggatt tagtgtagca gatgcctatg ccacaggagg acaagtggta gctgcagatc
    33001 aagttggaaa cacaggaaat tctaatggag agtctggaaa ttggcaagat aacttgaatc
    33061 aatatggtga acaagcaaaa gaagggctga aaaacttcgg cgatatggtg aaagaaggcg
    33121 ttcagggaat tggtgaagcg gctaaagacc tctggcggaa atatcaagga gagtagcccc
    33181 tttgaaaacg aataacccca tgttacaata gagagtaact actaaaagaa aagagggcat
    33241 aacatgtcaa acatctacga tagcgcgaat caaatcgaac gtgagatccg tgaattacca
    33301 gaattcaaag cgttgaaaga agcgtacgag aaagtacaag caaacgaaga agcacacaaa
    33361 ctgttcaaag acttccaagc gatgcaaatc gaattacaag aaaaacaaat gagtggacaa
    33421 gagttcagcg acgaagatgc aatgaaagca caagaaatgg ctgtgaaaat ccaatcagaa
    33481 gaagtaatca atgacttgat gcaaaaagag caaggcttca gtacaatcat caatgacttg
    33541 aaccgtatca tcatgacacc agttcgcgac ctttacagcg aataaaacta ttttatgaaa
    33601 caacagaggg tgtgaaagag acgttcggcc ttgagcgatg acaaaattgc ttcttattgc
    33661 cgataggctg tctcttgaac accatttacc aagaggtcgt gagcctaccg tttaactctg
    33721 agttacaagg aacaatttca aaaaacagct tctcatgttt gatgactatt gtgacttgtg
    33781 accgaggagt tggcaggaga acaccgttta ttcagaaata ggagctacga caaaagtctt
    33841 gtcatagctc ctattttttg tcattcattt ttcaagcggt agaaatagcc tttttgagct
    33901 aacagttttt cgtaagtacc tatttcaacg atttttcctt catcgatcac aacgatttta
    33961 tctgcctctt tgatcgttga aagccgatga gccacaataa aagtagtggg tgtttctgtc
    34021 aggtaattca gggcttgctg gatttttttc tctgactggt tatctaaagc tgaggttgct
    34081 tcatctagca aaatgatttt tggctgacgt atcaacgctc gtgcaatgga gatacattgc
    34141 ttttggccac cagacagctt gttgccatgt tcgcctacta aggtatccag accatcagga
    34201 agctggtcaa tcagtgaaga aagttgcgca gcttcgatga cttcatctaa acgttctttt
    34261 gatacgtttt tcaagccata agtgatgttc tctttgatcg aagcggagaa caacaaagtg
    34321 gtttgaggaa caacggctaa ataatttcgc acacttcgca tatccagttc atccaaaggc
    34381 tgatcatcta gaaaaatttt tcctgaagtc ggctggataa atccaatcaa taagttaagg
    34441 agtgtagatt ttccagagcc agatggccca acaaaagcaa ttgtttctcc agctttcacc
    34501 tctagagaaa aatgatcgat aatcggtttg agtgagtccg gataaagaaa agaaacatcc
    34561 tcaaaacgaa aatttccatc caaatgttgg atttttttct taccgtgata agtttctaca
    34621 tcgccagcaa tcaacagttc gccaatagaa gtgatagatt ccatgccttt tgaaatcgta
    34681 ggaatcagcg tgatcattcc agaaacggaa ttgacgatcg tcgtgaaata cgtctgatac
    34741 atcacgacat cgccgggttg gatgcgccct tgtaaagcta atatcccagt gaaaatcaaa
    34801 caaatcagct gaaatatctg aaaactagcc cagctgactg atccgaaaac agattgcagg
    34861 atatctaagt ggaatccttt ttcatagatt tgagtaaaat gcttatttaa gcgagttgtt
    34921 tcgtgttttt ctaacccatg agctcgagca atcggtgcta attcgatcac ttcgatcact
    34981 tttgcagaag tctcttccat ttcttgcctg aagtctgagt tggactgtct gatcctttta
    35041 cggaaaaaga gagtaacgaa tgcggcgaga ggaacagtca ataagaaaaa catgaaaaca
    35101 acaggacttt tataaagcgt gatacctaaa gctacgaata aattgacagc gatatttaat
    35161 ccgctgacaa atacttgaga ggacaacgtt tggatagctt ctacatcacg gatgatcttt
    35221 gactggattc gtccagattg caattctttt tgataaggaa tagatagttc ttggattttc
    35281 ttgataaggg tcgctcttag tccagcttct acttgacgga tcgaagtgct atggtatttt
    35341 acatgaaaat aattcgtcaa tatattttga ccaatcagac aagccatcaa aaaagcgttg
    35401 aaataaatgg cctgcaactg agaagaatct ttcgatgaag ctgcattgat tacattggca
    35461 atagcgatcg gcattaacca agatggaaga tgtttgataa taaaaaagag tcctgacatg
    35521 gcaaaacgcc aataatttcc ctgataaaga tgcagcaatg tctgaaaagc atttgcatga
    35581 tatttttgat aacttttctg tattcgttcc tccataatct tctccttaac aatcttggta
    35641 aataaactgc aagtgttcta ttgcttcagg aacataaatc tcaggagtag cttcaagagt
    35701 ggcgtacatt agaggacgct catatttgat ataatgcagt accgggcgga aatctaaaca
    35761 gcccttgcca acaggtacca tgcggatctt tccattttcc atgatatagt ctttgagatg
    35821 aagcgctgta atataaccat gtaaattgtc caaggcacct cggatcactt tttcctgatc
    35881 agcatgattt tctttatgca tcaaattcgc acaatctaaa atgattttta aatttggtga
    35941 agctacttca tcgattaaac gtttagctaa ctgatagtta tagattggat gattgatccc
    36001 tgcttcaaca gcaacagtta cgccaaaatt ttctgcatga gcgactaatt caatgataga
    36061 atttctggcg atctgataag cttcttcagt aaaattttct tttgtgtaac cgacagagac
    36121 actaccggtt tccgtagcaa tcatcttggc ttgataggca gagcacagag caatttgctg
    36181 tttgaaagtt gccaatgctt gtgcacgaac ttcttgattt ttgctggata ggttgatata
    36241 gcatcctaaa atggaaagtt cgatattgtt tttttggaag tattcaccaa aataggcagc
    36301 actcccaagt gtcatgtcag ttaatttaag ttgttcagga aatgcttttt gtggtgcaaa
    36361 ttggatatgg gacaagtgaa gctgtgatac tttttgaacg atttcttcac gatttcgaac
    36421 atctaaatcg tgggcacgga tgcctaaatt taatggaaac atctcaaact cctttttgta
    36481 aataattagc aagaggcaga cgggattctt ttatcaattc aacaattatt cgggcaactt
    36541 tatcagcgcc ttctggacta aagtgggtat tgtcttttag tccttctggg taatttttac
    36601 aagtatcagg cataagatgc aggtagaatt tttttgtctc ttcttttgag aaggtctgga
    36661 acaattcttg tgatcttgag aacacatcta aaagaggaat atggtgtttc tcagcaaatg
    36721 cacgcatcgc ttttggatag tctccaagta tatctggatt caatgttccg ttttctaagt
    36781 aatctcgtcg cgtcacagat gtcagtaaga ccatttggac gttcgctttt tttgcgcatt
    36841 caaaaaatat ctgcagattt tttaaatagc taccgaaggg aagcgtacct ctttcttctt
    36901 ggattttctg gtcgttatgt ccaaattgta caaataagaa atcattcggc tgaaactgtg
    36961 aagctagacg ggctaacctt ccttcctgga taaatgattt ggtgctgcgt ccattctttg
    37021 cttggttatt gatctggatc tcgggtgaaa aataagcaga gattttttct ccccaacctg
    37081 tctcgggacg cttgttttca tctttttctg ccgcagtaga atcaccggca atatggatag
    37141 taggcatgtt atctcttctt tcataaaggt ttataggcaa tgaagcagaa cagcaagaga
    37201 agaagacgga gcaa
//
LOCUS       DARBEC010000019        35398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.019, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000019 DARBEC010000000
VERSION     DARBEC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 35398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.019"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..649
                     /locus_tag="SCL80_000896"
     CDS             8..649
                     /locus_tag="SCL80_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106784.1"
                     /translation="MIERSTFLYVQLQENKTLLHFSPEFHLEGLTLGEIYQAEGLSAV
                     LHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTAAELQKQMRYQ
                     IDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSIQFPQLITMIH
                     RFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVAMEKAN"
     gene            708..1148
                     /locus_tag="SCL80_000897"
     CDS             708..1148
                     /locus_tag="SCL80_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106785.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            1160..1828
                     /locus_tag="SCL80_000898"
     CDS             1160..1828
                     /locus_tag="SCL80_000898"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEG1106786.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            1892..2515
                     /locus_tag="SCL80_000899"
     CDS             1892..2515
                     /locus_tag="SCL80_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEG1106787.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            2571..2957
                     /locus_tag="SCL80_000900"
     CDS             2571..2957
                     /locus_tag="SCL80_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEG1106788.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            3170..4561
                     /locus_tag="SCL80_000901"
     CDS             3170..4561
                     /locus_tag="SCL80_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEG1106789.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            5172..5540
                     /locus_tag="SCL80_000902"
     CDS             5172..5540
                     /locus_tag="SCL80_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEG1106790.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            5720..6661
                     /locus_tag="SCL80_000903"
     CDS             5720..6661
                     /locus_tag="SCL80_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEG1106791.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            6730..7461
                     /locus_tag="SCL80_000904"
     CDS             6730..7461
                     /locus_tag="SCL80_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEG1106792.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            7605..9785
                     /locus_tag="SCL80_000905"
     CDS             7605..9785
                     /locus_tag="SCL80_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEG1106793.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            9805..10287
                     /locus_tag="SCL80_000906"
     CDS             9805..10287
                     /locus_tag="SCL80_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEG1106794.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            10303..11064
                     /locus_tag="SCL80_000907"
     CDS             10303..11064
                     /locus_tag="SCL80_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1106795.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            11195..12529
                     /locus_tag="SCL80_000908"
     CDS             11195..12529
                     /locus_tag="SCL80_000908"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEG1106796.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
     gene            12836..13321
                     /locus_tag="SCL80_000909"
     CDS             12836..13321
                     /locus_tag="SCL80_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEG1106797.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            13314..13997
                     /locus_tag="SCL80_000910"
     CDS             13314..13997
                     /locus_tag="SCL80_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEG1106798.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGIILAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            14123..16768
                     /gene="polA"
                     /locus_tag="SCL80_000911"
     CDS             14123..16768
                     /gene="polA"
                     /locus_tag="SCL80_000911"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEG1106799.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            16813..17649
                     /gene="mutM"
                     /locus_tag="SCL80_000912"
     CDS             16813..17649
                     /gene="mutM"
                     /locus_tag="SCL80_000912"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEG1106800.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            17613..18242
                     /locus_tag="SCL80_000913"
     CDS             17613..18242
                     /locus_tag="SCL80_000913"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEG1106801.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEILVGQIENWLFANRFL"
     gene            18294..18626
                     /locus_tag="SCL80_000914"
     CDS             18294..18626
                     /locus_tag="SCL80_000914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1106802.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            18938..19429
                     /gene="nrdR"
                     /locus_tag="SCL80_000915"
     CDS             18938..19429
                     /gene="nrdR"
                     /locus_tag="SCL80_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEG1106803.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            19452..20825
                     /locus_tag="SCL80_000916"
     CDS             19452..20825
                     /locus_tag="SCL80_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1106804.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            20825..21751
                     /gene="dnaI"
                     /locus_tag="SCL80_000917"
     CDS             20825..21751
                     /gene="dnaI"
                     /locus_tag="SCL80_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEG1106805.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            21880..22320
                     /locus_tag="SCL80_000918"
     CDS             21880..22320
                     /locus_tag="SCL80_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1106806.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            22464..23165
                     /locus_tag="SCL80_000919"
     CDS             22464..23165
                     /locus_tag="SCL80_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEG1106807.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            23162..24283
                     /locus_tag="SCL80_000920"
     CDS             23162..24283
                     /locus_tag="SCL80_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEG1106808.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            24298..25530
                     /gene="pepT"
                     /locus_tag="SCL80_000921"
     CDS             24298..25530
                     /gene="pepT"
                     /locus_tag="SCL80_000921"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEG1106809.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            25662..26570
                     /locus_tag="SCL80_000922"
     CDS             25662..26570
                     /locus_tag="SCL80_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEG1106810.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            26605..26877
                     /locus_tag="SCL80_000923"
     CDS             26605..26877
                     /locus_tag="SCL80_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEG1106811.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            26888..27934
                     /locus_tag="SCL80_000924"
     CDS             26888..27934
                     /locus_tag="SCL80_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEG1106812.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            28185..30794
                     /gene="clpB"
                     /locus_tag="SCL80_000925"
     CDS             28185..30794
                     /gene="clpB"
                     /locus_tag="SCL80_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEG1106813.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(30945..31631)
                     /locus_tag="SCL80_000926"
     CDS             complement(30945..31631)
                     /locus_tag="SCL80_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106814.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(31609..32103)
                     /locus_tag="SCL80_000927"
     CDS             complement(31609..32103)
                     /locus_tag="SCL80_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEG1106815.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(32123..33343)
                     /locus_tag="SCL80_000928"
     CDS             complement(32123..33343)
                     /locus_tag="SCL80_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106816.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            33490..35325
                     /gene="lepA"
                     /locus_tag="SCL80_000929"
     CDS             33490..35325
                     /gene="lepA"
                     /locus_tag="SCL80_000929"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEG1106817.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT        11847 a         6250 c         7612 g         9689 t
ORIGIN      
        1 agatactctg attgagcgat ctacatttct atatgttcaa ttgcaggaaa ataaaacatt
       61 gctgcatttt tcaccagagt tccatctaga ggggctaaca ttaggagaaa tctatcaagc
      121 agaagggctg tcggctgttt tgcacttttt taaggtagaa ttggaattac cagtaaaaga
      181 ttatatcgag ctttctcttg aatcttggga tcgagcagtg gttgaactgt ttccagaagg
      241 tttaatgatc gatacaaacg atgggaagat ccatctgact gcagcagaac tgcaaaaaca
      301 aatgcgttat caaatagatg acgaaaattc tttttctatt tttagacgtc agcaaaagat
      361 tctaaaaagc tttttaaaag tgatcagtaa aaaacggaat ctgatcaaaa cgacacagct
      421 gttgaaaaaa tatccaggtc tgcttcacac gtctattcaa tttcctcaat tgattacgat
      481 gatccatcgt tttatacgta tgcaacagct accatctaag aagctgtttc ttccattgac
      541 ggacacattt cgagtagtca acagagaaga taaaaagaaa gtgattgtga ttgactttac
      601 cagaaatcgg aatgtccttc accaagttgc aatggaaaag gccaattaag gctttttctt
      661 tttggatttt tttgtcataa taaatattag atgatggagg agataatatg atgcaaacat
      721 ggttagaaag gctaatacga tttcagcaga actatcacaa acttatgcag ggaagatatg
      781 cgaaatttga tcagttgaat cgcggtctat tgatcgctgc gcttgtcttt agtttgctaa
      841 gtcgttggtt gccctataga atcggacaat tgttgttcat cgtctttttc ggatggatga
      901 tatttcgttt cctatcaaaa aaaatctacc ccagactaaa tgagaatcaa cgttatttga
      961 aatatcttga tagagtcaaa gaaaaatatc atcagataaa aaacaaaaag caagtcaaaa
     1021 tggatcgaaa aagttatact ttttttgagt gcccgaattg tcatcaaaag cagcgtgcac
     1081 ctaaaggaaa aggacgaata cgtgtgacat gcaaaacatg tggtataaaa ttcgaaacga
     1141 atgtttaagg gagaaaagaa tgaaattagt attattacgt catggcgaaa gcgaagcaaa
     1201 ttttgaaaat tactggacag gatggctaga tgtcgcctta acagaaaaag gaaaagaaca
     1261 agcagcagtt gccggtcaaa aaatgcttga agccggactt caattcgatg ccgtttatac
     1321 ctctgttttg aaacgtgcga ttcttaccgg acaagtcgca ctagaagaaa tgggtcagtt
     1381 atggcttcct ataataaaaa catggcgtct aaatgaacgg cattatgggg atcttgtggg
     1441 taaaaacaaa gataagatga aagaaatata cggaaaagat caagtaaaaa catggcgcag
     1501 aggatatacg gaagttccgc cgttaaccgc agaaaataga tttgaccgca gatatgatca
     1561 cttggatccg catttgatcc catacggaga aagtctggaa atgacagtga atcggatcat
     1621 tcccttatgg caagatcatc tagcaccaaa actaaaagat cggcaagatt tactagttgt
     1681 gggtcatggt aatagcatcc gagcactgac aaaatattta gaagatgttc ctgaagatca
     1741 aatggatacg atcgatatcc caaatgcaca accaatccag tatacattag cagatgattt
     1801 gacaattctg gacaaaaaaa tactttaaag aacaccttta tctaaaagtg agagatagaa
     1861 aaaagaagca aaggaatgga acaaaaggaa catgaaaaat tatatatggg actttgatgg
     1921 tacattgttt gatacgtatc ccgcaatggt agatggtgca tggcaagcac taaaagattt
     1981 tggtatcagt atggacaaaa aagaaatcta cttcaaaatg aaaaagtatt ctactagtta
     2041 tttgatcaat gaatcaaact tgaatgccag agagtttaat gaattatttc accgatatga
     2101 aaaagaaagt acggaagttt cccgtccatt tccagaaacg aaacaagtac tggaaatgct
     2161 aaaagacaac ggcggtcgtc atttcatcct gacccaccgt ttgacagaat caacatgggg
     2221 cctgttaaaa gaacatcgat tggcacatct gattgaggaa gtagtaggga tcgatcagga
     2281 ctttcctcgt aaacctgatc cagcatcttt aaattatttg atcgatactt ttcatttaga
     2341 aagaacagac acgatgatga tcggcgatcg tcgtttagat atcgaagcag ggaaaaatgc
     2401 aggagtcgct acttgtcttt acgacatcga tcattttctg ggtgagatcc cggcagatta
     2461 tgtggtagga aatctgaatg aaatccttac cttgcagagc atcattaaaa agtagtatcc
     2521 cctttcaaag gagatatgtt ataattttca taaaggagtg ataaaattat atggaaatca
     2581 gaagacaaaa agaagttgta gaagcatttc attatgatat gagattgccg aaccaagaag
     2641 tagaaacaga tttgaaagta ggcttctcgc ctttaaaaga aacgatcgaa aactatccga
     2701 aagaacatag catcgtagga gcacgcttag agttccgttt agtgtttgat acatatgttt
     2761 tatctggaag tgtgagtcag atcaatcata tcctcaatcg gaaaattgaa ggtcaaaatg
     2821 atgtcacaca agaagaagtg gacgagctag tagcaccgtt attcgatatc gtacgccgaa
     2881 tgtcttatga ggtgacagag atcgccttag atcagcctgg ggtaaaattg aattttcaat
     2941 cagaaaaaca agaatagaca gtaaaaacat acctgcgata gattaaagct atcacaggta
     3001 tgttttttta ttactaattg taagcaaaaa gaatgtcatt tcgataaaaa tcgtctaaaa
     3061 tataaaagtc actgaaaaaa attttttcag gaggatggtt gaagaatcat tctcgggaac
     3121 ctttacttcc ttcgatggac ttgtcattaa taatatgagg aggagaaaaa tgacaacact
     3181 catcacagtt ttagtggttc tagtttttgc ggcattatta tatgtgttct atcgtatgca
     3241 aaagcgccat acaaaatttt caacacgcgt ctttacagca ttaggagttg gtatcgtctt
     3301 tggcggtctg cttcaattga tctttggtgt agacgacaaa gtcgttactc aatcaatgga
     3361 atggatcggg attgtaggtc aaggatatgt atcattcttg caaatgctag ttatgccttt
     3421 agtgtttatt tcgatcattg gtgcattcac aaaaatgaaa gtttctgaaa agctagggaa
     3481 aattagtgcg actgtattga cttctttact tggaacaaca gctattgcag cattgatcgg
     3541 aattggttca acaatgctgt tcggactcca aggagctaaa ttcacacaag gagctgcaga
     3601 aacagcacga atctctgaac tggcaacacg tcaggaaaca gttgaaaact tatcgattcc
     3661 acagcagatt ttatccttta tccctaaaaa cgtttttgct gatttagcag gtagtcgtgc
     3721 aacaagtacg attggagtcg ttattttcgc ggcatttgtc ggaatcgctt atctaggcgt
     3781 gaaaagaaaa gcgccaaaag aagcagcatt cttttcagat ttagtcgaca gtctgtataa
     3841 aatcattatg cggatcgtga cgcttgtttt gcgtttgaca ccttatggag tatttgcttt
     3901 gatgacaaac gttttggcaa caagtgattt tgcagctatc gtcaatttag gtaaatttgt
     3961 cgttgcttct tacgtagcat tgcttgctgt tttgattgtc cactcactga ttttgattgg
     4021 tgcaaaagtc aatccaatca actatttcaa aaaagccttt ccggtattga gctttgcgtt
     4081 tacttcacgt tcaagtgctg gagctttgcc attgaatatc gagactcaga caaaagcttt
     4141 aggagtagat gatgcaacag ctaatttctc agcaagtttt ggtttgtcta ttggacaaaa
     4201 cggctgtgct ggcgtttatc cagctatgtt ggcaacgatc gtcgcaccag cagctggagt
     4261 gaatgtcttt agtttggaat ttattttgat gcttgtggca attgtaacaa tcagttcttt
     4321 tggtgtagct ggagtcggtg ggggcgcaac atttgcttca ctgatcgttt taggggcaat
     4381 gaacttgcca gtagcaatcg ttgggttagt tatctctgtt gaaccattaa tcgatatggc
     4441 acgtacagca gtaaacgtca gcggtagcat ggtagctggt atcgtgacaa gtgcacgcat
     4501 caaagattta gatcgcgatg tcatcaatga tgattctaaa gtgatcgatg tgaatgcata
     4561 gtcaaaaaga ggttgtgata gaagtggtca gctttaagaa ataagaagaa gtttcctaaa
     4621 aatagtttct caaatttttg tgaaatttcg gcttatttcc gagaagctac ttctgaaaca
     4681 ccgtgaataa gaggttgtga aaaagctgcc ctgcatcaag taataagaac agccaaacaa
     4741 aaatagttcc tcatatttta cgtttggttg agcttattac cgcagatgca agcttttgaa
     4801 caccgtttaa gaaagaggtc gtgagcctgc cgtttcactc tgagtcacaa agagcaattt
     4861 caaaaaatag cttctcatgt ttgttgaata ttgtgtcttg tgaccgagga gttgtcagga
     4921 gaacaccgtt taccagaggt tgtgacttgt ttttagtcac gacctctttt tttatttatt
     4981 ccatataaat acacattatt tattccgaaa agtgaaagcg caaataacaa aaacaaccaa
     5041 cttatttatt attaaagaaa agcgtattta agtaggacga ttctgttgag agctttcgta
     5101 gattttccca ttttcctatg gtaaactgac aaagttgaga atgttagaac agatcgttta
     5161 ggaggaaaca gatgtttacg aatgaaataa aaatcatgct ttatgtggac gatgtagaaa
     5221 aaaatgcaac gttttggaga gcagttggct ttgcggaaaa agatcgtcaa gaaatggatg
     5281 ggacgcttat aattgaagta ggagttgagg acagtcaaac ctcatttctg ttatatgatc
     5341 gagcatttgt tgagtcccat tcacctggca cagctactag tgccccatct ttgatgtttt
     5401 acagtaaaga tatctttgga ctatataaaa aaatgcagac gcaatcagtc acattaggtg
     5461 atatggtaca gcttggagaa gaatatgtct ttaactttgc tgatcccgat ggcaactact
     5521 ttgcggttac aggtaaataa tagaaataaa gactttgttt ttctgtgcga aacaggaaag
     5581 cgaagtcttt ttaattcaaa taataatatt atgtaaacaa aaaactttcc atgcgtaatt
     5641 atacacaagc aaataaatcg tcaaatgcga acgccttctt tatactgttt aggaacgaaa
     5701 agaaggaagg aagaaataca tggattatca agtactgctt tattacaatt atacaaaaat
     5761 cgaagaacca gaggaatttg ccaaaaatca tttagccttt tgcaaatctt tggatcttaa
     5821 aggtcgtatt ctagtagctg aagaaggaat aaatggtact gtgtctggaa ccgttgaaaa
     5881 cacgactgcc tatatgaaag caatgaaagc tgatcctcga tttgctgata cgatgttcaa
     5941 gatcgatcca agcgaaggac atgcctttaa gaagatgttt gtaagatacc gtccagagct
     6001 tgtaagttta aagctagaag aggacattga tccaaaccaa gtgacaggtg cctatctttc
     6061 accgcaagaa tttaaagaag ctattttaga tgaacagaca gtggtgattg atgcacggaa
     6121 tgattatgaa tatgacttgg gacattttcg aggggctgta cgcccagata tacgcagttt
     6181 tcgagagctt ccagactgga tccgcgagca caaagaagaa tttatggaaa aacgggtagt
     6241 gacctactgt actggtggca ttcgttgcga gaagttttct ggttggcttg tccgtgaagg
     6301 attcaaagat gtcggacagc tgcatggcgg tattgctact tatggtaagg atcctgaagt
     6361 ccaaggagaa ttatgggacg gcaagatgta tgtgtttgat gaacggatcg ctgtcgatat
     6421 caaccaagta gataaacaaa tcgtcggtcg cgactggttt gacggaacac catgtgaacg
     6481 atatatcaat tgtagcaatc ctgagtgcaa tcgtcagatt cttgcttcca ttgaaaatga
     6541 agaaaaacat ttaggagcat gtagcataga gtgtgcgaaa catccgcgca atcgctatgt
     6601 tcaagaccat ggaatctctc cagaagaagc agaactgcgg tttacagctt taattaaata
     6661 gaaaacaata gttttgattg tcgaccattt ccaatatgcg ctatactagc gttgaggatg
     6721 gtgatccaca tgaaatcatt gatttatgct attggggatg tccatggcaa acatgagctt
     6781 tttgaaaaac ttattcacta ttatgatcca aagatccatc agcttgtttt attaggggat
     6841 ctcaatgatc gaggtcctcg tacaaaagaa tgttttttat taggaaagca attagtcgaa
     6901 gaaacaggag ccatttacct ccggggaaac catgaagaat attttttgca atttctgcaa
     6961 tcaccggagg actggtatgc gccttatatg agaaatggtg gaaaagaaac gtttgaaagc
     7021 ctgattcacc caggggcatc ggaggaatac tccccaactg aaatctcgat gatgatccaa
     7081 tcgagatatt cagaactgat taaattttta gtccagcgcc ccttgtatta tgaatgggaa
     7141 aattatcttt ttgtccatgc cggtgtggat ttgacaaaga aagactggcg taagacaagt
     7201 ctaagagact ttatttggat aagggaacca tttcatgaag ggaaaaacaa tacaggtaag
     7261 acaattgtat ttggacatac gattacacca atgctccatg gagatatgca gacaacagac
     7321 ctctggattc aggatcataa aatcggaatg gacggtgggg ctgtattcgg agggtcattg
     7381 catggggttg tttttgacaa ggaaggtatt cttcaagata ttgaatatca gaacaacgaa
     7441 ggaccttggc atggaatctg actaatggaa agaatgaagg agctgtgact ttgttgcggc
     7501 tctttttatt tttatagaga atattgtttc tttcagtagt ttaacccagg ataaaaagta
     7561 tgatagaata aagaagcaat ttttagcaga aaagaggcat tttaatgacg gaaaatttga
     7621 accagacgat cattcagctt gtacaaaaag aactttcaga ctatcgtcct aagcagttga
     7681 ctactgtact taatttgtta aatgaaggaa acactgttcc ttttatcgcg cgttaccgaa
     7741 aagaaatgac tggaagtctt gatgaagtcc agattcgtga aatcgaagag cggtatgcct
     7801 atcttgaaaa cttagaaaaa cgtaaaactg aagtgattcg gctaatcgat gaacaaggga
     7861 aactgactcc cgagttagaa gcagaaatca cccaagccgt gaaaatgcaa caagtggaag
     7921 atttgtatcg tccatacaaa caaaaacgac ggacaaaagc aacgattgct aaagaaaaag
     7981 gacttgaacc actcgcgaag tggctgatgc agcttacaga tggagaggtg caatctgaag
     8041 cagaaaaata tattgacaaa gaaaaagaag tctcttctgc cgaagaagcg ttgcacggtg
     8101 ctcatgagat tattgctgaa caagtcagcg ataatgcaaa atttcggact tggattcggt
     8161 cttatacgta taacaaaggt atgtatgtca gcaatgtcaa ggatgagcaa gcagatgaaa
     8221 aaggcgtata tgaaatgtat tatgactttg cagaacccgt ccataaaatg gtttctcatc
     8281 gaatattagc tactaatcgt ggagaaaaag aagatattct gaaagtattc tttcaagtag
     8341 atgaagcagc aattttagct tacttggatc gccaaatagt caaaaatcct gcctctcctt
     8401 cttcttcatt cgtcagagaa gcctatcagg atagttataa acgctttatt cagccagcaa
     8461 tcgagcgaga actgcgcaat gaattgactg aaaaggcaga tgagcaagca atcgctattt
     8521 tcggcgaaaa cctccgtaat cttttgttgc aaccgccact aaaaggtaaa gttgtcctgg
     8581 gatttgatcc ggcttatcgt actggatgta aactagcagt cgtagatgcg acagggaaag
     8641 ttttagcaat cgaagttatt tatcctcata aacctgcagc gcaagcaaaa agagaagctg
     8701 ctggaccagc ttttatccaa ttaattaata aatatcaagt agatatggta gctatcggaa
     8761 atggtacagc cagtcgggaa tctgaacttt ttgtggctga gcagttgaaa tccgccgacc
     8821 ataaaaccta ttacgctatc gtcaacgaag caggcgcttc tgtttactcc gctagcgaaa
     8881 ttgcacgaaa agaatttccg catctacaag ttgaagaaag aagtgctgtt tctattgccc
     8941 gtcgcctgca agatccttta gctgaattag tcaaaatcga tcctaaagct gtcggagtag
     9001 gccagtacca acatgacgtt tctcaaaaac gtttggctga acaattggat tttgttgtgg
     9061 aaacagcggt taaccaagta ggtgtggatg tgaatacagc aagcccgcag ctcttgcagc
     9121 atatatcagg attgaataaa acaactgccc aaaacattgt catttatcgt gaagaaaacg
     9181 gagaattcac cgcacgaact caattgaaaa aagtgccacg cttaggacca aaagcatatg
     9241 aacaagcaat cggattcttg cgagtccctg gtgggaaaaa cattttggac aacacaggta
     9301 tccatcccga aagttattct attgcaaaag aaatattaac ttcagtccaa ctttcagaaa
     9361 aagagcttgg tacagaagaa gcagtagaaa aattgacacg attatccgta gagaaactgg
     9421 ctgaaagcct atcagtcgga gaagaaacgt tagccgatat tttagctggg ctgacacaac
     9481 ctggacgaga tatgcgtgat gagatgccag cgcccttatt acgtacagat gtattgagta
     9541 tggaagactt gaaaccaggg atggaattga ctggaacggt ccgtaatgtc attgacttcg
     9601 gcgcatttgt tgatatcggt gtgaagcaag acggacttgt ccatatatcg aaactcagca
     9661 aaaaattcgt caagcatcct acagatgtcg tgtcagtagg agatatcgta acagtttgga
     9721 tagaacaagt cgatacgaaa aaaggcagaa tcagtttgac gatgctttcc ccttatgaag
     9781 agtaacaatg aggagtaaca aaaattgaca caagaagaac tgcagcagtt agtagaagag
     9841 atatcattaa aaagcttcca gcttcctttc aagcaccaag caatgttcaa tccacgctta
     9901 cgtacaactg gcgggcgcta tcatttaaac gatcatcatt tagattttaa tgcgaagcta
     9961 tttgaagagt cagacgaaga gacgattgca ggaatcatca aacatgaact ttgccattat
    10021 catcttcatc tagctggaag agggtatcag cataaagata tggatttcaa gtatcttttg
    10081 aaaaaaacag gagggttgcg ctatgcacca gccttgacga agaaacagat aaagatggag
    10141 tattatcgat gccaagattg ttctgccacg atctgccgca agagaagaat cgatgtacgc
    10201 aaatatcgtt gtggaagatg tcatggagaa atcaaatgga ttgagacaaa aagagaagac
    10261 tttaatgaag cacaaaaaat cagatgaaag gatagaaggc ctttggaaaa aagtgagcag
    10321 aggaatctaa aagactggaa tcagctatta aattttttag ccgcacctga agagggaaat
    10381 tttgaggaac tgacggcacc tacacttatt ttagctggaa actgtcttcc tatcttagct
    10441 gataaagcag cacaaatgta tttaaatggc caagtagatc aattgttttt ggtcggaggg
    10501 gtggggcatg cgactagaat cctttatgag aattttgaaa agcaaggatt tcattttgaa
    10561 gatggcatga gtgaaagtga aatctgtcgt cagtatctaa aagaagtcta tgatttgccc
    10621 gataaggctt ttctgataga atcgaagtct acaaattctg gagagaacgc cactttttca
    10681 ctggagattc ttcattcctt aggagctgta ccagaaaaaa tcttattgat gaatgatccg
    10741 acactgcaac gcaggactcg agcaactttt gagaaagtat ggcaaaatga acaaactatt
    10801 ttcgcgaatg ctgtgccatt tgtgccggaa atccttcact tttctgaaga gatcatcttt
    10861 acagcaaaag aactgaatca tcaatggcct aaggaatatt tccatgcgct agtattagga
    10921 gaaatggaac gtctccatga cgatgagaac ggctatggtc cgaaaggaaa agattttatt
    10981 cctcacattg atatatcaga ggaagtttgg tcttcttaca ccagaattaa agagtcaata
    11041 aaaacggatt tttctcgtac ttgaccattt tagcaagcga atcctccttt ccaaataaat
    11101 ttggttgaaa ttttcaatcg tttataagat aatgaggagc gtgttgaatg taatagctaa
    11161 aagaatggac gctagataag agaagaggat tcaaatggaa aatcaaaata aattgtatca
    11221 ttttgttggt atcaaaggtt caggaatgag ctccttggct cttgttttac atgaacaagg
    11281 tctaaatgtc caaggatcag acattgaaaa atatttcttt acacaaagag atttagaaaa
    11341 agcaaatatc acgattctgc catttaacgc agataatgta aaaccaggga tgacgatcat
    11401 tgcaggaaat gcatttcctg attcacatga agaaatccag cgagcaaaag aattaggttt
    11461 ggaagtgatt cgctatcacg actttattgg tcattttatc caaaattata cgagtatcgc
    11521 tgtaacaggc tctcacggaa aaacaagtac gactggactg ctctctcatg tattgagtgg
    11581 tgtccgtcca acaagttatc taattggaga tggaacagga catggtgatc cgcaagcgga
    11641 attcttttca tttgaggcct gtgaatatcg ccgtcatttc cttgcctatt caccagacta
    11701 tgcaatcatg accaatatcg attttgatca tccggattat tatacaagta tcgacgatgt
    11761 atttacagct ttccaaacaa tggctggaca agtgaaaaaa gctatttttg cgtatggtga
    11821 cgatgcttat ttacgcaaat taaaagcaaa tgtgccgatt tattattatg gtgtcacaga
    11881 aaacgatgat atccaagcac gaaacatcga acgcacaact tctggttctg cttttgatgt
    11941 ctatcatggg gatgaatttg ttgggcactt cactgtacca gcattcggta aacacaatat
    12001 cttaaatgct ttaggtgtta ttgcagtagc ttatttcgaa aaactggatc tgaaagaagt
    12061 tgcagaagaa atgctgactt tcccaggagt aaaacgtcga ttcagtgaaa agatcgtagc
    12121 agacatgacc gtagtcgatg attatgcgca tcaccctgca gagatcaaag caacgattga
    12181 tggcgcacgc caaaaatatc cagataaaga aataattgca gttttccaac cccatacgtt
    12241 tacgcgtacg attgcattga tggatgaatt tgcagaagca cttgatttag ctgataaagt
    12301 ttacttatgc gatatttttg gttcggctcg ggaagaacaa ggaaacgtta agattgaaga
    12361 ccttggagcg aaaatcaaaa aaggtggaga agtcatcaaa gaaaataatg tatctccatt
    12421 gttagattat catgatgcag tagtaatctt catgggtgct ggagatgttc aaaaatttga
    12481 acaagcgtat gaaaaacttt taagcagcac gacgaaaaat gtactgtaat acaattactg
    12541 cagtgtcctt ataaaaatag aaagaggggg agtggcaaaa atcttgccaa gccccctttt
    12601 ttgcaactta tgataaacgg tgttcaaaag cttgcatctg cggtaataag cccaatcaaa
    12661 tcaaaaacat gagaagctgt tttttcttgg ctgttcttat tatttgatgc aggacagctt
    12721 tttcacgacc tctgtaaact ataggaatct gttaaatcaa gggagcagaa cttgcctttt
    12781 acgctacttt tggtacaata atgtatgatt aaaaataaac tggagggttt ctatcttgaa
    12841 cataggtaag ccgagaaaac taggaaaaac aataaatgaa atcgaggaag gcgactcgtt
    12901 atccttaaca gaatctattg aagataacca gcttttgctg tatttgggat tgacgaatga
    12961 tgcaaatcct ttgtatatcc aacatgactt tgcacaaaaa acagaatatc aacagccaat
    13021 cgttccatca gtgatgctga tggggatcat cacaagtgca atttcaaagc atcttcctgg
    13081 accaggatca catgttgtca atttttctgt caattttatt gaaccggttt tccattatga
    13141 aacactgact tttcagtttg aggtcataaa agtagataag atgaaggatg tggtgacgat
    13201 ttctgtagaa gcggagaata cgaacgaaga tcgtgtgttg gatgctgtcg ttatggtcca
    13261 accgccacaa gttcctgaat tggaaatagc aatcgaaagt gaaggtgaat tgaatgaata
    13321 atccaagtac atcaatgtca ggtatcaatc gtttttatgc aaaagtttat ggatttttag
    13381 gtatgggact tgcccttagt gcagtcactt cttacttggc tctttgggta tttcctttgc
    13441 aattagcaac ttttatcaat aattttccac taggctttgt gggtctgtgg atcgttgaac
    13501 tgattttagt aatggttcta ggcgtaaaag cacaaaagaa tccgagctta gcaatcggtg
    13561 gatttattgc ctactctctt ttgaatggca taatattagc ggttactctt gcatactacg
    13621 atattggaac agtaacgcaa gctttcttga cagctacttt gatgttcatg gggatgtctg
    13681 ttgtcggagt aacaacaaaa agagatctta gcggggttgg aagagcagga caggcagctt
    13741 taatcggagt gatcattgca ctagtcttga acatcttcat tttgagaagt agcgtagttg
    13801 agctgttcat ctccctactg ttagtcgtca tctttgcagg gattacagct tatgataacc
    13861 aaagcatccg taatatctat gtccagacaa acggaacagc acatagtgga attgctgtat
    13921 tcatggcatt gaagctgtat ttggacttta tcaacttgtt cttggcgttt ctgagaatct
    13981 ttggacggaa caactaaaaa agaaaaaaat tcatcagttg gggctatacc gaattatttg
    14041 gtatggtccc gctttttctt ttcgcattcc gcaggattct ttgttagaat gaataagaac
    14101 tttacagtaa ggagttaaga agatgacaaa aaataaatta ttactcgttg atgggaacag
    14161 tgtggctttt cgcgcattct ttgcgcttca caattctctt gaacgtttca aaaatagaaa
    14221 cggtcttcat acaaatgcaa tctatgcatt caatacgatg tttgaaaatg ttatgcaaaa
    14281 agagcaaccg actcatgttc tggttgcgtt cgatgcagga aaaacgactt ttcggacaga
    14341 aatgtatgcg gagtacaaag gtggaagatc caaaacacct ggcgaattca aagaacaaat
    14401 gccatatata cgtgatttgt tgacaggact aggggtacag tattacgaat tgcccaatta
    14461 tgaagcagat gatattatcg gtactttggc agaaaaagta gacaaagatc aatttgatgt
    14521 cgtcgtgctt tcaggtgatc gggatttgac tcaattagct agtaaagaag taaaagtgga
    14581 tatcactgta aaaggtgtca gcgatatcga aagctataca ccagaacatg tagcggaaaa
    14641 atatgatggg ttgacgccaa aacagattat tgacatgaag ggactagccg gagatgcctc
    14701 tgacaatatc cccggtgtga ctaaaatcgg tgaaaaaaca gctatcaagc tattgaaaca
    14761 atatggctca gtcgaaggaa tttatgaaca tatcgatgaa atgaagcaaa gcaaaatgaa
    14821 agaaaatctg ataaatgaca aagagcaggc tttcttatcg aaaaaattgg cgaccatcaa
    14881 tacttcagcg cctgtggatg tctctatcga ctcattgaag tatgaaggga aaaatctcga
    14941 taaactcgtt ccgttttata aagaaatgga tttcaatcag ttcctttcta aattgaatat
    15001 cgtagaagaa gaagtaaaaa tggatgatat tttgtttgaa gtcgttcatg aattcaaaga
    15061 ggaaatgttt actacggata tggcgttgta cgtggaaatg atgggcgaca attatcatac
    15121 agaggaaatc gtcggagtag cgtgggggac agaaaagaaa atttatgtca cgaacgatct
    15181 atctgtattc gaaaaccgag cgttccattc ttggatcact gatccgactc ggctgaaaaa
    15241 agtatatgat gcgaaacgaa cttatgttgc tttaaaccgc tatactggaa aaacgaaagg
    15301 aatcgatttt gatgtcttgc taggtgcata ccttctcgat acgaatgaaa aaagcacaga
    15361 tatcgaagga atagctgctc attacggata caacgatatc caatcagatg aagctgtata
    15421 cggcaaaggg gcgaaaaaag gtttaccaga agaagaggaa ctcttttttg ctcatttagc
    15481 gcgaaaagta gctgcaatca atgcgttgac accaaaattg agccaagaac tggcagaaaa
    15541 aaaccaagaa gatcttttcc ggaaaatgga actgccattg tctatcattt tggcagagat
    15601 ggaaataagc gggatcaaag tagatgcgac tcgcttacaa gaaatgaagg gcgagttctc
    15661 tgctcgctta agagagatcg aacaaaaaat ctacgaagaa gccggcgaag aattcaattt
    15721 gaattcacca aagcaattag gagtgatctt atttgaaaaa atgggattac ccgttatcaa
    15781 aaaaacaaaa actggatatt ccacagccgt agatgtactg gaacagcttc gtgaacaagc
    15841 accaatcgta gaggatatcc tcacctatcg tcaaattgca aaaattcaat ctacctatgt
    15901 cgagggactg ctgaaagtga tccaatcaga tggaaaagtg catacccgtt atgtccagac
    15961 attgacccaa acaggacgat taagctctgt tgatcctaat ttgcagaaca tcccgattcg
    16021 attagatgaa gggagaaaga tacgtcaggc atttgttcct agagaaaaag actggctgat
    16081 ttattcttct gactattcac aaatcgagtt acgcgtactt gcgcatatct caaatgatga
    16141 acatttgaaa gctgccttcc tagaaggtca agatatccat gccagcacag cgatgcgtgt
    16201 atttggaatc gaaaaggcag aggatgtcac accaaatatg cgtaggcagg caaaagcagt
    16261 gaattttggt atcgtgtatg gcatctctga ttacggactt tcacaaaatc taggaatcag
    16321 ccgaaaagca gcacagcaat acatcgatac gtactttgaa aaatacccag gtgtcaaaga
    16381 atatatggaa agaatcgtac gcgaagcaaa agaccaaggc tacgtcgaaa cgctgtatca
    16441 ccgccgtcga tatctgccag acatcaattc aagaaattac aatatccgtt cttttgcgga
    16501 acgaacagct atcaatacac caatccaagg aagtgctgct gatattttga aaatcgcgat
    16561 gatcgaattg gataaacgat taaaagaaac aggtcttcaa gctacgatgc tgttacaagt
    16621 tcatgatgaa cttgtcttcg aggtaccgga aaaagaactt gaatcattag ataaattggt
    16681 gaaggaagtt atggaacaag ctgtttctct tcatgtacca ctgatcactg acagcagttg
    16741 gggaaaaacg tggtacgaag caaaataaac attgtttaag acagagcaat ataatgaagg
    16801 tgaaaagaaa aaatgccaga attaccagaa gtagaaaccg ttcgtaaagg attggaacgg
    16861 ttagtagtag gaaaaacgat tcaaaaagtt caggtcttgt ggcccaagat catcgagcaa
    16921 ccagagacac ccatctttga ggcttctctt gttggcgaaa ccatccaatc cattggacgt
    16981 agagggaagt ttttaatttt tcatctcgat cattgtgaac tgatctcaca cttgcgaatg
    17041 gaaggaaaat atcaatttac gaaagaaaat acaccaatcg ataaacatac gcatgtactt
    17101 ttcttttttg aggatggaag ccaacttcgc tacaacgatg tacgaaaatt tggtcgtatg
    17161 acaatcgtcg aaaaaggagc aagtgcaact taccgaggaa tcatgaaatt gggacctgag
    17221 cctttgccag atagcttctt attagcagat ttcgcaaatg gactgaaaaa gtcccacaaa
    17281 gcaatcaagc cattgctgct agaccaacgt ctagtgactg gtctaggtaa tatttatgta
    17341 gatgaagcat tatgggaagc gaagatccat ccagaacaac cggcgaacac actgcgcccc
    17401 aaagaggtag aacaattacg tctatcgatc attgatgttt tggatcgtgc aatagaagcc
    17461 ggaggtacca cgattcgcag ttatttgaat gcattaggag aatcaggcgg atttcaagta
    17521 gcactgcatg tctatcagca aactggcaaa ccatgtatac gttgtggaac gccgatcgtg
    17581 aaaacaaaag tggcacaaag ggggacgcac tattgtccga attgtcaaag gctaaaaatc
    17641 gcaaagtagg atttgtttta ggtttgacgg gtggaatagc taccggtaaa agtacggcag
    17701 ccaaagtctt tcagtctcat ggatttcctt tgatcgatgg cgatgtgatt gccagagaaa
    17761 ctgttgagcc tggaacgctt gctctgagga aaattgtatc agtgtttggc caagaggtat
    17821 tgcagcctga tgggcaacta gatcgaggga aacttgggat gattgttttt ccatcaaagg
    17881 aaaaacggca aaaacttgat cagctattag atccgtttat ccgaaaagcg ataaaagatc
    17941 aaatcgcctc tctaagttcc aatcatcctt tagtaattgt agatatccca ttgctctacg
    18001 aaggtcatta tgaccactat atggatgcag tagctgtcgt ttatactacg ccagaaactc
    18061 agcttcagcg tttgatgaaa cgtaatcaac tgacgctaga acaagcgcag cagagaatta
    18121 gtagtcaact tccaatcgaa gaaaaaaagc agcgtgcgga tattttgttc gacaacaatg
    18181 gaacaaaaga aatacttgtg ggacaaatcg aaaattggct ttttgctaat cgctttcttt
    18241 gagtatctgt gtcaattttg ctatcctgag tatatagaaa gtgagggata gcgatgggat
    18301 cattaaaaaa tcaaaaggat aaagtcgtag gtaacgcaaa agaaaaaatc ggcgagatgc
    18361 tcaataaaga aaatctaatc gatgaaggtc gtacgcaatc agaaacagca gaagaaaaag
    18421 aaaagcagta ccaagcaaga atcgatgaaa tagaagacca gcaggcagaa gaagaagcga
    18481 agcggcaaga tttgactcaa gaacgaaaaa atccgacacc tgatcgtagt aaaacaccgc
    18541 tcccagaaag tgaccacatt cccgatgagc aaaaaatcga ggaacagcga atgaaacact
    18601 atacaggaat cgaagagaag ttataaaata gtaaaaaatc gagatgtgac ttgaagcaga
    18661 tgagaaggtg ttcatctgct tcaagttttt tgtgctagat gactaaatca gcttttgtgt
    18721 aagttatttc tcagtaatca aggtgatcag tattggaaaa ttgaaattta gtaaggacag
    18781 gggacacaat tacgcatgaa agtagaaaaa attgttctta tatccgtgtt ttttgggtag
    18841 gacagtcgcg tttgtgattt gtttcgtgtt ataatacaac tagttgtaaa gaaagacaca
    18901 tcctttatat aaaatgcgat gcaagaggtg agacaaaatg agatgtccca gatgtcaaca
    18961 taataattca cgagtaatcg acagccgtca agcagatgat ggacgtgcga tccgtcgccg
    19021 tcgcgaatgt gagaattgtt cgttccgatt cactacattt gaacgaattg aagctgctcc
    19081 gttattagta atcaagaaaa acggggaacg cgaagagttc aatcgcgata aaattttaag
    19141 aggactcatc cgttcagcag aaaaaagacc tgtagctatg gaacaaatgg aacaaatcgt
    19201 caaccatgtc gaaaaccgcg tacgcagtct cggagaaaac gaagtatcga cgaatctaat
    19261 tggtgaatat gtgatggaag atttagttga tttagacgaa atcgcttata ttcgttttgc
    19321 aagtgtttat cgccagttca aagatatgtc tgttttctta aaagagttgc aagatatcgt
    19381 ggataaagca aaagaaaata ataaagagaa taataaagaa aacaattaag aaacaaaaag
    19441 gaggcatttc cttggaaaat gcttggaaag agcttcaacc aaaaaatatt taccaaggaa
    19501 gaaaagcggc tccgctgaga gaagacagca agaacgcctt gctgtatctt tatcagccac
    19561 taatcggcgg agaagccttg agtatctatt tcaccctgtt atcagaaatc tctcaagaat
    19621 ctggcaaagg tcctgaaggg atgcatgcag atctgcttag tagtctaggc tgcggtatcc
    19681 cgcaatttta tgatgcaaga aaaaagctag aaggaatcgg cttactggat gtttatttta
    19741 aagaagatag tgatctggga cctcgtttta tttatgagct gttagaacca ctcattccta
    19801 aagcattttt caaagactct gttctggctt ttcttcttct ggaaaaagtt ggtgaacgac
    19861 gattccagaa gcttacccaa ttctttgaac ctaaaaggca tacagcagag ggatatcaaa
    19921 aaatcacaaa aaaattttta gatgtttatc gcttcaatga agcagtgtac gctgctaatc
    19981 aagaaaacat cgaatcgctg aataaatctt ttgaagaatc tgaatcagta gcattacttg
    20041 aagcagattc gacattcgat tggcagtttt tgaatgattg gctggcaaaa aaacatattg
    20101 caagtcttga tgatccaatc atcaaacaag tcgacatgta tcatcagctt tacggtttcg
    20161 atgaattgac acttggcgaa ctgatcagtc aatcttatga ctttagtgaa caaaaagtat
    20221 ctgaaaaaga actgcaaaaa gccattttgg caatgcagag acgtccggtg aagcaaacaa
    20281 aagaaacagt tgcggtttct gaaaacagta atgaaccctc gtcaaacgaa catttgacaa
    20341 acgcagcgct tcagctgata aaagaagcac agagcattcc accgatgaaa tatctggaag
    20401 caatcaagtc agaaaaaggc ggatatgttt ctaaacaaga aaactggtta ctgcaagatc
    20461 ttgtttctca atcaggtctt tccagtagcg tgatcaatgt tttgctgaat tatgtactag
    20521 tgatcaagaa taatgctagc ttaaatgcca gttatgtaaa tacagtggca aatgaatggg
    20581 cacaaaagaa aatcgccaca gcagaagaag cgatccagca cattcggcaa ataagtaatc
    20641 aagcaaaaca atcaaaacaa aagaagcaaa caaattatca aactggcaag cgaaatgttc
    20701 gacgggaaaa gcttcctgat tgggtcaacc agccaaaaga tgaaaaacag atcagcagcg
    20761 agaaaaaagc agagattgac cgccgtttta aagaatacct ggcaaaaaag gagggtgata
    20821 tctagtggaa gacgtaggca aagaattgaa gaggatcatc agcaaacgca attatcaaga
    20881 aaagtatgct gaaatgatcg ctgaagtctt aaatgatgcg gatgttcaag cttttctaag
    20941 cgcacaccaa gaagaattga cacaagcgga tattgaaaaa agttacgcta aattgtatga
    21001 attcgtgcaa gaaaagcgta aatatcaaaa aaacgatcca acgatgatcg ctcccggcta
    21061 tgagccgaag ctgacgttga attttcatta tgtggatgtc acttatgtac caacggaagc
    21121 attgttagca cggcaaagac aagaagaaat cagaagccga gtaaaagcaa tggatatgcc
    21181 aaaagatatt caatcggctt cttttgaaaa ctttgaacgt acagatggac gaggatatgc
    21241 gagcttagaa gccctagatt ttgtagagaa atatactgct gatccaaaag ggtatcataa
    21301 aggactgtat ctagtaggaa gttttggtat tggaaaaaca tatttattag gagcaacagc
    21361 gagagaatta gcgattgctg gattcaccac gacgctagtc cattttccga catttgccgt
    21421 tgagatgaaa caggcaatcg gaaaagatca agtggcagaa aagttagatg cagtgaaacg
    21481 ttcacctatt ttgatgatcg atgacattgg tgcagatgcg atgagtagtt ggatacgaga
    21541 cgaagtattc ggtgttatcc ttcaatatcg gatgcaggaa cagttaccaa ccttcttctc
    21601 atcgaatttt acaatggatg aattggaaca gcatttgtcc gtcactcaac gtggagatga
    21661 agagccgcta aaagcgaaac ggataatgga acgcatacgt tatttgacaa aagaaatcga
    21721 aatgactggt cgcaaccgac gcaatggttg aacagttgac aagtccaact gacatttgac
    21781 ctagtagtaa aagcaggtca gcgaatcatt atatggacag aaatgaccgg agacaatcac
    21841 cgcaacaagt aatgcagaaa cttaaacgga ggatttttta tgagggctat tttttctagt
    21901 atatctgtag ttggtctatt ttttctttta gcaggctgcg gtagcgcaga tagcaacaca
    21961 ggttctgttg attcgattga tgcgggtcaa acagtaaaaa caagcagtac gaaaacggag
    22021 agacaaagca tatttgtcgg aacgattgaa agtatcactc aagtagaagc agaagaagaa
    22081 acgcttcagt tgaaactcgt cgatgtcatg gccaaagaag atccagcgaa tatgggaacg
    22141 tcattcagca atgacggagt gatcttaaat gctactgctg aaaaaataag cgtggacata
    22201 aatgaattga aaatcggtga caaagtccaa tttacattag ttgagatgcc gatcatgacg
    22261 atgtctatcc caccgcaagt tcctggagat tcgattattg atgtttcatt gaacaaataa
    22321 ccaataacat tttttcaaaa ataagaaaat gttgttttat agaacgaaaa ggtatatttt
    22381 ttaaggagat atgggaattt ttttattcct agtctcctta ttttatttgg aaaatgctat
    22441 aattatctag aaggagtgaa gctatgaacc atttagattt atctcagcgc ttagccgcag
    22501 tgggtgactt tgtccctgaa tccgctcgtt tagcagacat tggatcagat cacgcttact
    22561 tacccgttgc attgatatta agaggaaaaa tcgaatatgc agttgctggt gaagtcgttc
    22621 aaggtcctta tgaatcagca cgtaagcaag tcaaaaaaga tggattagaa gaccgtatca
    22681 ccgtacgcct tgcaaatggt ctagatgcta tccagccaca agatggtatt acagctgtca
    22741 cgattgcggg tatgggaggt tcactgatcc gagatatcct agaagctggc aagttgaatc
    22801 aacgcttaag cggacaagaa cgtttgatcc ttcagccaaa catcggagag cgtacattac
    22861 gatcttggct gcaagaaaat cagtataaaa tccaatcaga aaaaatactt gaagaaaata
    22921 aaaaaatata tgaaatcatt gtagccgaaa aagaacaaaa accagttact tacaatgaaa
    22981 tggagctgat gttcgggcca tttctgcttg aagagaaaag tgagatcttc aaatcaaaat
    23041 ggacaagaga gcttcaacaa agaaaagcta tccgtgaaca gctcaaacaa gcagctaatc
    23101 aagaggcacg tcaaaaacaa atcgagcagg aaatccaaat gattgaggag gttttaaagc
    23161 catgacaatc agcggacgca ctttcatcca aaagtttgaa agctattgtc ctcagtggct
    23221 agctgaagaa ggagacccag tcggactgca tatcggaaca ttggataagc caatccaacg
    23281 agtaatgatg acattagacg ttcgtccaga agtggtcgaa gaagcaatca agaaaaaaat
    23341 cgatttgctg attgctaaac atcctccaat ctttcgtcct gtaaaacggc tggttactga
    23401 tcagccacaa gaaaaaatgt atgcagattt gttgaaacat gatattgccg tctatgcagc
    23461 acacacgaac atggatatta tttgggatgg actgaatgac tggttttgtg aacttctcgg
    23521 tattgaagtg gagagttatt tagtaaagac tcatgagatt cattataaaa aactggccgt
    23581 ttatgtacct gtggatcatg cacagaaaat gcgtgaagta ttagctgcag ctggtgctgg
    23641 tacacaaggt gattataccg gtacaagttt tacctcgatt ggacacggtc gatttactcc
    23701 ggaagctggt gcacaaccag cgattgggaa agtagggaag acagaacaag ttcaagaggc
    23761 aaaagttgag gttattttgc cggaaactat cgaaaaacaa gtgattcaag ccatgcggtc
    23821 agctcatcct tatgaggaac ctgcttatga tcttttcgct atagatgaac cagtcgaaat
    23881 gtttgggtta ggacgagtag gtgaattgcc gcaagaaatt tctatagagg catttgtaga
    23941 acaagtaaaa gaagcgttcc aactagatgg actgagaata gtccagccaa aaaatgcaaa
    24001 atcctctgtg aagcgcattg cgatttgcgg tggaagcggt gaaaaatttt atccgcaagc
    24061 aatcgcccaa agagcagatg tctatatcac gggagatatc tattatcata cggctcatga
    24121 tatgcaaagc gctgggttga ttgcaattga tcccggtcat tatattgaat cgttatgcaa
    24181 gcaacgattt atcgagaaat ttgaatcttg gaaacaagaa gaaaattggg atattaactt
    24241 tttcgtttct gaaacaaata caaacccatt tcaatttaat taaaaggaga gaatactatg
    24301 tacgaaaatc ttttgccacg ttttttacga tatgtcaaaa cagaaacacg ctccgatgca
    24361 acaagcaaaa cgactccatc gactcagaca caagttgctt ttgcgcaaga attgaaaaaa
    24421 gaattagaag agttaggctt agctgatgtt ttctataatg aagataacgg attcgttatg
    24481 ggaacattac caagcaatgt tgatcatgaa gtgcgttcca ttggatttat ttcccacatg
    24541 gatacagcag attttaatgc agtaggagtc aatccacaaa tcattgaaaa ctatgatggc
    24601 gagtctacga tcgtactaga tgcggaagaa cgctatacat tgaatacaaa agattttcca
    24661 aatttgaaaa attatgaagg ccacacattg atcacaacag atggcagtac attactggga
    24721 gcagatgaca aatcagggat tgcggaaatc atgactgcaa tggagatctt gatcaataat
    24781 ccttctatcc ctcacggtac catcaaagtg gcttttggtc ctgatgaaga aatcggtgtc
    24841 ggagcggata aattcgatgt tgagcaattc gatgtagatt ttgcttatac agtcgatggt
    24901 ggtcctttag gtgagttgca gtatgaaacg tttagtgctg ctcaagcaaa tattacgatc
    24961 caagggaaaa atgtacatcc agggacggca aaagatacaa tgatcaatgc tctacagtta
    25021 gcaatcgatt ttcataatca gcttccagcc gatgaagttc cagaaaaaac ggaaggatac
    25081 gaaggtttct tccatttgat ggcattggat gggaatcctg aagaagcttc tatgagttat
    25141 atcattcgcg atcatgatcg tgaaaaattc gaagcacgca aagcacaaat cacacatatc
    25201 caagaagagt tgaatcagcg atttgatcaa gaacgaatca aagtagatat gtatgatcaa
    25261 tattacaaca tgaaagaaat cattgaaaaa gatatgagca tcgtggaatt agcgaaacaa
    25321 gcaatgatcg agcttggtat ctctccagtg atcgaaccag ttcgtggagg aacagatggt
    25381 tcaaaaatct cttacatggg tattccgacg ccaaatctat ttgccggagg agaaaatatg
    25441 cacggacgat tcgaatttgt ttctttacaa gtaatggaaa aagcaacaga cgtgattgta
    25501 aaaattgcag aattgaacac aaagagttaa aatcgaaaaa gaacagctta tgacaaggcg
    25561 ttcgtcccaa tgatggaatg tcatctgtct aaggctaaag ggcaggacaa tggaattggt
    25621 tgtcccgccc tcttttttga agagtaaata ggaggtaaag aatggaaaag ctgctagttt
    25681 tttataacaa aagttcaggg aaagatgagg gtgagaaatt agcacgctgg tttcaagatt
    25741 atgtaaaaga aaagcagcca gaattatatg tagaactagc tgaaactggc ccatcgattg
    25801 atgatgcgga attagtcaag aaagcagaag ccatagaagc tgatacttta gtaattatag
    25861 gtggagacgg cacgattcat catatcgtcc aagcgttcca aaaaacatta tctcaatacc
    25921 atgtcggatt acttccaggt ggaactgtca acaaccttgc acgtgttctt gagattcccc
    25981 tcaaaaaaga agaagctgct gacgtgatcc tggaaggcaa tgtacggaaa atagactatg
    26041 gtcaagtcaa tgatgaagtc attatctcta cactgacaat cggaatctta gcagatacag
    26101 cagtttggat cagtcaagaa gaaaaacagc gttacggctc gtggatcttt atcaagaagt
    26161 tttttaagtt attgctgaag aagaaaaaat atcatttgga tttagaaaca gacgaggagc
    26221 attggaaagg aaaatcccaa ctgttgacag tcacgatgtc taactccgtg ggcggtttta
    26281 caaattttga cgactcagcg accctagatg atggaaaatt tcatgtaacg atcgtacctt
    26341 ccttagatat tttccgattt attttttatt tgcctagaat catgagaggc aaattctata
    26401 cgatccccgg cattacttat ttcacagcca aacgcatgaa aatccaagcg caggaaaaat
    26461 ctgtgggcac tcgaacggat ggagacacga cagatgatct tccagttgaa ttatctgttg
    26521 tatcaaaagg attaacagtt tattcaccaa agaaagaaga aaaagcttag aaatagtagt
    26581 taacagaaag ttggaagaag agtaatggat gtattgttac acaatccttt gtggttttac
    26641 cttttcattg tgaattttta cttattttgt ctgatggggt atgataaaca tcaggctaga
    26701 actagcggct ggcgtgttcc tgagaataat ttgatatttg tcagcctgtt aggcggtggt
    26761 ttgggaggat tgatctctcg aaaagttttc catcataaaa ctcggaaaaa gaagtttact
    26821 ataggattcg ctgcaggtgt acttgttgat ttgattctca tttatttttt ccattaagga
    26881 gcaggagatg tcgtctaaag gaacgaaaac taaaatcatt ctgaatctgc ttttgctgat
    26941 tgtgattttt gccattattt attatttaat caatcaatca tttgcagata tctttgccga
    27001 attgctgtct accagtgttg ttgttttggc agcggctgtt atattaggga cagtttacca
    27061 aatcgctgaa gggcgctcaa tcaaagaaat tgctgaacct ttcgcgccga attttacaac
    27121 gaaagatggg ttcttgacct ctagctatat tgcgttttac cgaatcgttt cgtttggtac
    27181 aggaacgctg atctcggaga tttattttta taacaaaaaa ggaatgaagc tatcccaggg
    27241 agcaggggtg acagcactcc atatgattat gtacaaactg gcagtgatca cttatgcatt
    27301 agctggcttg gtcatccaat tctctctttt ttattctaat ggtccatcaa tgatctggtt
    27361 tatcatcgct ggtatccttt tgacgatcgt gattgttgcc tttttattgg cgatgtccgt
    27421 cagcttgaat gttcaaatat tttttgtcag cttatcgaat aagttattta aaagtgagcg
    27481 tttgcggaaa tgggtggata catgcaatca gcagatctac tctttaaggg aaacggtcca
    27541 aaccattatc caagacagga ctgcgctatt gcggatttat ctatggaata tgttaaaact
    27601 tgtattttgg tatgtacttc cttatttgat tctgattgaa aaccatccta aaatagattt
    27661 tctactgacc atttcgttta tcagctttgc agtagtgctt tcaggggtta ttcctacgcc
    27721 tgctggtatt ggttcctttg aatttgtcta tttgttatta ttcagaccag ttgttggaac
    27781 agttgacgca gtttcttcgc ttttactgta tcgtttcagt tcctttatct tgccatttct
    27841 ctatggattt gtgtatgtga ttgctgaacg gcgaagagtg atcaaagaag aaataataga
    27901 agtaaaaaaa gagaataaag aaaatcaagg gtaggtcttt tataagatct acccttttta
    27961 tcaaatcaaa gaaaaacgtt atggataaga taattttacg tttgtttggc tcttttttta
    28021 tatacataca atcaattttg tttttttaag atttatataa gtcgagaaaa aaagtcaaaa
    28081 aactatgtgt atattttgac caaaattgac caaagcgata taatagtttt tgtaaagatc
    28141 aaagaaataa gtgatcttac ctttaattag gagggtatac ctgtatgaat attgagaaaa
    28201 tgactacgac gcttcaagaa gcgattgctg aagctcaaca gattgcggtc actcgaaaac
    28261 atcaagatat cgacattgcc catgtatgga aaatcttttt acaatctaat cattttggtc
    28321 gaaatttcta tacggatgcc ggattggatg tcgaatcttt tgaacatgaa attgatcgtc
    28381 tactggatga gtatccagtt gtttctggtg gaaacgtcca atatggacaa aacctcagcc
    28441 agaatttctt tcgattattg aatgaagcag accaaatacg tgaaagcttc ggcgacgagt
    28501 ttctttcaac ggaagtcgtt attttagcgt tgatgaaatt aaaaaattat ccgttaactg
    28561 tttatttgaa taaaaacgga ttatctgaaa aagaattgcg taaaaacatc gaagagatga
    28621 gaggaggaga aagagtgacc tcacaaaacc aagaagaaca atacaaagca ttagaaaaat
    28681 acggcgttga tttagtacag caagtcaaaa gcggaaaaat ggatccaatc atcggtcgtg
    28741 atgaagaaat ccgtgatgtc atccgtattc tttcacgaaa aacaaaaaat aatcctgttc
    28801 tgataggaga acccggtgta gggaaaacgg caatcgttga agggcttgct caacgtatcg
    28861 tacgcaaaga tgtgcctgaa aatttaaaag ataaaacgat tttttcatta gatatgggtg
    28921 cattgatcgc tggagcaaaa ttccgtggtg aatttgaaga aagactaaaa gcagtcttga
    28981 aagaagtcaa aaaaagtgat ggacgtatcc tcttgttcat cgatgaaatc cacaatatcg
    29041 taggggcagg aaaaacagaa ggaagtatgg atgccggaaa tctgttgaaa ccaatgcttg
    29101 ctcgcggtga attacatttg atcggtgcca caactttaga tgagtaccgt caatatatgg
    29161 aaaaagacaa agcgttggaa cgtcgctttc aaaaggtatt agtcaaagaa ccaacagtgg
    29221 aagatacgat cagtattctg cgtggattaa aagaacgttt tgagatccac catggtgtta
    29281 acatccatga caacgcatta gtagcagcag ctactttatc tgaccgttat attactgatc
    29341 ggttcttacc ggacaaagca atcgatttga tcgatgaagc cagtgcaacg attcgggtag
    29401 aaatgaactc tatgccaacg gaattagatc aagttactag acgtttgatg cagttggaaa
    29461 tcgaagaagc tgcactgaaa aaagaatcag atgatgcaag taaaaaacgc ttgaaaaatt
    29521 tgcaagaaga attagctgaa cttcgtgaag aagcaaatgc gatgaagatg cagtgggaaa
    29581 cagaaaaaga agaagtcaat tctgtttcag caaaacgtgc tgaaatcgat aaagcaaaac
    29641 acgaattaga agatgcagaa aataactatg atttagaacg cgctgctgta ttgcgtcatg
    29701 gtacgatacc tcaattagaa aaagaattga aggaattaga agctaaagca aaagacagcg
    29761 agattaagat ggttcaagag tccgttaccg aaaatgagat tgctcaagtc gttggcagat
    29821 taacgggtat ccccgtaacg aaacttgtag aaggtgaacg agaaaaacta attaaattga
    29881 atgaaacact tcataaacga gtgattggtc aagatgaagc agttgatgcg gtcagtgacg
    29941 cggtaattcg ttcacgggct ggattgcaag acccaaatcg tcctctaggt tctttccttt
    30001 tcctaggacc aactggtgtc ggaaaaaccg aattagcgaa agcgttagct gagaacttgt
    30061 ttgactcaga agatcatatg gtacggatcg atatgagtga atatatggaa aaacatgctg
    30121 tgtctcgtct agtcggagct cctccaggct atgtaggtta cgaagaaggt ggtcaattaa
    30181 cggaagctgt cagacgaaat ccttatacaa tcgtcttgtt ggatgaaatc gaaaaagcgc
    30241 atcccgatgt cttcaatatc ttgttacaag tcctagatga cggacgcttg actgattcaa
    30301 aagggcgagt agtagatttc aaaaatacgg ttctgatcat gaccagcaat atcggttcac
    30361 agttactgct ggaaggcgtg actgctgatg gaacgattcc agaagcagtg gctgaacaag
    30421 tcaacacgct attacgtgga aacttcaaac cagaattctt gaaccgaatc gacgacacga
    30481 ttttatttac accattgagc ctagacaacg tgaaaggaat cgttgacaaa atggtggcgc
    30541 aattggcaca acgtctggaa catcaagaaa tcctgctgac aatcagtgat gaagccaaaa
    30601 cgtggattgc tgaaaatgct tatgaaccag catatggtgc aagaccattg aaacgattta
    30661 tcactaagga agtagaaaca ccactagcta aagaaatcgt tgcaggacat gttatgccta
    30721 aatcaaaagt aacgattact ttacttgatg gacaacttca tttcaaaaca gaagagctag
    30781 aagaaatcgt ctaatcttcc attgagacta taaaagaaaa aaggggtctg acacaactag
    30841 tgtcagacct ccttttttca aataaacagt gttccagaag tagtttcttc gaaaaataag
    30901 ccgaaatttc ttgaagctgc ccgcttctgt cacaacctct tttattaggt atttttcact
    30961 ttttctaatt tataactttc aaagtaacgc tcatcttttt tactttgaga ccatgcaaac
    31021 aatcccatta aggcagcacc tatcgtattt gtcagcaagt cgcccatcgt atccatcaat
    31081 gctgctcgtc cgacaaacag tgtgccatca gaagcagcaa aacgttgaag attcatgcct
    31141 aagaactggt cgcataagaa ttcccagaac tcccaaaaca cgccgcaaag tccagcaaac
    31201 gcaaagccga ataagaggaa caaccaagga ctggtttttg atatctcagc atctttcatc
    31261 aagtaaccaa tcaatccata gccaagagct gtcaggacca ttggactcac cgcatgtaag
    31321 attttgtccc aaaaagagat gatactgatc aaatgcatcc ctgtgcctaa aaagacagag
    31381 atgaacaaga aaaaccagta aaacaaaacg attgctgctg gtattttaag tttgaaaact
    31441 ttcaaaatga cttgtggcat gaaaatcaat gcaatcccta ataaagattc cacgataaat
    31501 gtgattcctt gagaagtcga atatttttgt gtgataaatt cataaaaatt atagcagaca
    31561 acgaatacac caaaagcaag taaagctatt tgcacctttt tgatttcttt aatcactgaa
    31621 agtccctcca tctaaaaatc tttttacttc tgaaagcaac tcaatttctg aagaaaccac
    31681 ttttccgttc aatttgatca aacctattgt atataagttc aggtagtgaa attgattttc
    31741 agctgtttct tgtagtgcag cgattttttt cgggtgggtt ccgccttgct gtcgagtatc
    31801 agtatataag ccgatcactg ggatgccttt cgcataagcc acaccgattt ccgatgctac
    31861 cccggcatcg atcgtcagcc catctagtaa agcaatcatt aaatcactag ccaacacttt
    31921 ttctgtgtca gcaagagcaa tcattcgact gtctgcataa gcagttttgt cattgattgc
    31981 ttcattttct tgaggaagat agatcgaaag ctcagaagag agctctctga tttctttaac
    32041 taactgttgg ttatataaca aatcactttt agcaaacatt ggtgcggcaa aataaatttt
    32101 catctaaaca tcatcctttt tatcaaaaat aatcgctaaa gtaagcttcc atgtttggaa
    32161 tgatacgctc tagtgacttc agtgtttctt catctgtttc taaccgttcg attcttgcca
    32221 aataagaagc acggcgataa ttcatcatca agcaagtcaa tgtttggata tccagtcgaa
    32281 cggattttcc tataggttca tttaagacac ggacttgtcc ctgttcatcc catgttaaag
    32341 cgaagacacc attattccaa ggagcgacag gatcttcaat gatgaaatga aaagcatcag
    32401 ctgttccaac aaacggaaac cgttgaagga attcttttac atcaacgatc cgagccataa
    32461 aatatggttc gatctgctct ttgatctgac tatcttcaat caaaaatgct aaaggctcat
    32521 ttttataaat atctccatgt acccagtaga tcatggaaaa atgtgcggaa ataaagttcc
    32581 ataagccatt tcgagcttct tgatcaagat agaacatttc ttttacgtga aatatttctt
    32641 cagctaccca gtagaacaga acacctcttg gttcatggtt gctatcgtag tagacagcgg
    32701 ctgtgcgctc atcttcattt tcgaaacgcc aatactcttc ccaatgaaaa tcactgcgga
    32761 ataaagcacc atggttttgt tgggcaaatt tgtcataaac ggaaaatacg tctggatgat
    32821 caactggtaa tcgctcaacc ataccactta ctgggatttg tttaggtagc tgtgtatctc
    32881 tgattttaaa ggaaagttta tcagacatga tttcccatcc tttacgccga tagtagggga
    32941 tactgtaagg atacaaataa gagatccatt gcttattctt gcgcatatta tccaatgcaa
    33001 cgataattag atcttgcatc aatccatggt tagcatattc tggataagtt ccaactccag
    33061 tcacaccacc cattttataa cgtgcaccgt gaatattgac ttcacaagga tagatagcga
    33121 tttgagagat cagtttattt tcgtggaacc atccgaacac tttcgatact tccaaaatcg
    33181 gttttttgga acggataaaa gctcgtttat tctcaaaccc gctttcctcg atgtctgctt
    33241 cagtcacttg gaacacatag gaaagcaatt cattgaattg atcgatatgt tcttgagtga
    33301 ccggttttaa ttcaagattt tctctaaaat ctttttgatc cattttttca tctccattta
    33361 ttagcttgat aacagtatag caaaattcta caaaaaaaac ttctatagct gactaattaa
    33421 aaactcttta aattactgct tttcgttggt ataatggaaa gtgctgatta tatgaaaaga
    33481 ggagacgcta tggatataaa gaaaatgaaa gaacgccaag agcagattcg taatttttcg
    33541 atcatcgctc atatcgacca tgggaaatct acattggccg atcgtatttt agaaatgacg
    33601 cacactgtga cgtctcgcga aatgcaagat cagctgttag actcgatgga tttggaacgt
    33661 gaacgtggaa tcacgattaa attaaatgct gtagaactca attacacagc gaaaaatgga
    33721 gaaacatata tctttcactt gattgacacc ccagggcacg tcgacttcac ctatgaagtc
    33781 tctcgaagcc ttgctgcctg tgagggtgcc gttttagtgg tcgatgcagc acaaggaatc
    33841 gaagcacaaa cattggccaa tgtttattta gcgttagaca atgatttgga gatcctgcca
    33901 gtcatcaata aaatcgacct acccgcagca gatcccgaac gtgtccgtca agaaatcgaa
    33961 gacgtaatcg gaatcgatgc tagtgaagct gtattagcca gtgccaaatc agggattggg
    34021 attgaagata ttctcgagca gatcgtggaa tatgttcctg caccaagtgg agacttagat
    34081 gcaccgctaa aagctttgat ctttgactct atttatgata gttaccgcgg tgttgttcta
    34141 aatgtccgga tcacggacgg tatggtcaaa cccggagaca aaatcaaact gatgagtaat
    34201 ggcaaaacat ttgatgttac agaggtcggg gtcttctcgc caaaagcagt tgctagagac
    34261 tttttgatgg tcggtgatgt cggttatatc acagcaagta tcaaaactgt ccaagataca
    34321 cgagtagggg atacagtaac gttagcagac aatcctgccg ctgaagcttt accaggttac
    34381 cgaaaaatga atccgatggt ttattgtggt ctttatccaa ttgatacttc tcgttacaac
    34441 gatttgcggg aagctttaga aaaactgcaa ttgaatgatg ctgctttaca attcgaacca
    34501 gaaacctctc aagcattagg ttttgggttc cgctgtggat tcctcggact tcttcatatg
    34561 gatgtcgtcc aagaacgctt ggaacgggaa ttcaatctag aattgatcac gactgcacca
    34621 tccgttattt accatgttaa taaaacggat ggtacgactg ctacagtaga taacccggca
    34681 gattttcctg aacctgtaac gattcaggat gtagaagaac cattcgtcaa agcacagatc
    34741 atggtcccaa atgattttgt cggtgcagta atggaacttt ctcaacgaaa acggggagaa
    34801 ttcattacga tggattattt agatgattac cgagtaaatg tcgtctataa tattccgctt
    34861 tctgaaattg tctttgactt tttcgacaaa ttgaaatcaa gcacgaaagg ctatgcctca
    34921 ttggattatg aaatgtctgg ttatcaaaaa agcaagttag tgaagatgga catcttgctg
    34981 aatggagaaa aagtcgatgc attaagcttt atcgttcacc gagactttgc ctatgaacgt
    35041 ggaaaagcaa tcgttgaaaa actgaaaaaa ctaatcccac gccaacaatt tgaggtgcca
    35101 atccaagcag ctattggaca aaaaatcgtt gcccgttcag acatcaaagc cttgcgtaaa
    35161 aacgtcttgg ctaaatgtta tggtggagat gtttctcgta aacgtaaact cttagagaaa
    35221 caaaaagaag ggaagaagcg gatgaaacaa atcggctccg tcgaagttcc gcaagaagcc
    35281 ttcatggccg ttctgaaaat ggacgaggac gaaccgaaga aatagcaagg gtttgtttga
    35341 aaacagtgta aaaaataaac catgtttaca tggatttgcc accggtttgc caccggtg
//
LOCUS       DARBEC010000020        34636 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.020, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000020 DARBEC010000000
VERSION     DARBEC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34636
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.020"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            591..1280
                     /locus_tag="SCL80_000930"
     CDS             591..1280
                     /locus_tag="SCL80_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1106818.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1267..2475
                     /locus_tag="SCL80_000931"
     CDS             1267..2475
                     /locus_tag="SCL80_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1106819.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2769..4040
                     /gene="serS"
                     /locus_tag="SCL80_000932"
     CDS             2769..4040
                     /gene="serS"
                     /locus_tag="SCL80_000932"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEG1106820.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCL80_000933"
     CDS             complement(4589..6073)
                     /gene="guaB"
                     /locus_tag="SCL80_000933"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEG1106821.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6578..7273)
                     /locus_tag="SCL80_000934"
     CDS             complement(6578..7273)
                     /locus_tag="SCL80_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEG1106822.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCL80_000935"
     CDS             complement(7363..8463)
                     /gene="ychF"
                     /locus_tag="SCL80_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEG1106823.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8472..8663)
                     /locus_tag="SCL80_000936"
     CDS             complement(8472..8663)
                     /locus_tag="SCL80_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEG1106824.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8684..9574)
                     /locus_tag="SCL80_000937"
     CDS             complement(8684..9574)
                     /locus_tag="SCL80_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEG1106825.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9561..10328)
                     /locus_tag="SCL80_000938"
     CDS             complement(9561..10328)
                     /locus_tag="SCL80_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1106826.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCL80_000939"
     CDS             complement(10340..11053)
                     /gene="rsmG"
                     /locus_tag="SCL80_000939"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEG1106827.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCL80_000940"
     CDS             complement(11285..13186)
                     /gene="mnmG"
                     /locus_tag="SCL80_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEG1106828.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCL80_000941"
     CDS             complement(13203..14600)
                     /gene="mnmE"
                     /locus_tag="SCL80_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEG1106829.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14899..15282
                     /locus_tag="SCL80_000942"
     CDS             14899..15282
                     /locus_tag="SCL80_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106830.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15450..16208)
                     /locus_tag="SCL80_000943"
     CDS             complement(15450..16208)
                     /locus_tag="SCL80_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEG1106831.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16226..17044)
                     /locus_tag="SCL80_000944"
     CDS             complement(16226..17044)
                     /locus_tag="SCL80_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1106832.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCL80_000945"
     CDS             complement(17041..17397)
                     /gene="rnpA"
                     /locus_tag="SCL80_000945"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEG1106833.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCL80_000946"
     CDS             complement(17515..17649)
                     /gene="rpmH"
                     /locus_tag="SCL80_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEG1106834.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
     gene            18172..19506
                     /gene="dnaA"
                     /locus_tag="SCL80_000947"
     CDS             18172..19506
                     /gene="dnaA"
                     /locus_tag="SCL80_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEG1106835.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDLKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            19704..20834
                     /gene="dnaN"
                     /locus_tag="SCL80_000948"
     CDS             19704..20834
                     /gene="dnaN"
                     /locus_tag="SCL80_000948"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEG1106836.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            21063..21305
                     /gene="yaaA"
                     /locus_tag="SCL80_000949"
     CDS             21063..21305
                     /gene="yaaA"
                     /locus_tag="SCL80_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEG1106837.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            21292..22416
                     /gene="recF"
                     /locus_tag="SCL80_000950"
     CDS             21292..22416
                     /gene="recF"
                     /locus_tag="SCL80_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEG1106838.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQTRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDNRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            22413..24359
                     /gene="gyrB"
                     /locus_tag="SCL80_000951"
     CDS             22413..24359
                     /gene="gyrB"
                     /locus_tag="SCL80_000951"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEG1106839.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            24379..26850
                     /gene="gyrA"
                     /locus_tag="SCL80_000952"
     CDS             24379..26850
                     /gene="gyrA"
                     /locus_tag="SCL80_000952"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEG1106840.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
     gene            27086..27385
                     /locus_tag="SCL80_000953"
     CDS             27086..27385
                     /locus_tag="SCL80_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEG1106841.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
     gene            27435..27947
                     /gene="ssb"
                     /locus_tag="SCL80_000954"
     CDS             27435..27947
                     /gene="ssb"
                     /locus_tag="SCL80_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1106842.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            27973..28209
                     /locus_tag="SCL80_000955"
     CDS             27973..28209
                     /locus_tag="SCL80_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEG1106843.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            28352..30325
                     /locus_tag="SCL80_000956"
     CDS             28352..30325
                     /locus_tag="SCL80_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEG1106844.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            30331..30783
                     /gene="rplI"
                     /locus_tag="SCL80_000957"
     CDS             30331..30783
                     /gene="rplI"
                     /locus_tag="SCL80_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEG1106845.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            31057..32424
                     /gene="dnaB"
                     /locus_tag="SCL80_000958"
     CDS             31057..32424
                     /gene="dnaB"
                     /locus_tag="SCL80_000958"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEG1106846.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            33204..34496
                     /locus_tag="SCL80_000959"
     CDS             33204..34496
                     /locus_tag="SCL80_000959"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEG1106847.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
BASE COUNT        10554 a         6733 c         6844 g        10505 t
ORIGIN      
        1 ggagtgtttc ccgattcgtt tttggctgcc gtagcaactt ttatatcata tcagagagac
       61 aagtaattgt caactctttt ttaaaagttt tttcgatttc gtttttcgaa atcttccagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacgact cagttatctt atcatgtcat
      241 ttccaacatg tcaacaactt attttttatt atttaataag ttattggtgt tcttttgaac
      301 aaataataat atatcaacat cacatgtgaa agtcaataag aaaatgctta tttatcaaca
      361 tttctagctt acatgatcat tggttagttc tggtcctttt tcgttcgtct ttataactcc
      421 gagagacgta aaatcctttt gttttcagtt taatgttcat gttcttgtct tattcacacc
      481 aactagatgt ctcaccttct aaaattttcc ttaacaaagt gctgctttta tctatttctg
      541 cttttttttg ctagaataat agtaacaata tgactctgga ggatttattt atgaaaattt
      601 tgattgctga cgatgataaa gaaatcgttg aattattaag tatctatgtt cacaatgagg
      661 ggtatgaagc agtaaaggct tatgatggga aagaagcatt atctaaaatc agaacaatcc
      721 ctgatattga attacttatt ttagatatca tgatgcctga gatggatggg atgcaagtag
      781 tgaaagagct acgaaaagaa tcccaaatac caatcattat gctgacagca aaaactactg
      841 atatggataa gatcaaagga ctggtcgctg gtgcagatga ttatgtgacc aagccattta
      901 atccgttaga agtaatggct agagtaaagt ctttattacg gcgtacaaaa atgcagattg
      961 ccccagatca gcctgatgta ttagaagtaa atgcattaat catcaataaa gactctcatg
     1021 aagttaaaac aatcgatggg aaagaaatcc agttaacagc tttggaattc ggtatcctct
     1081 acttgcttgc tagtcatcct aatcgagtat ttagcgcgga tgaaattttt gagcgtgttt
     1141 ggaaacaaga aagtattgtc tctgctaaaa ctgtcatggt tcacgttagt catttaagag
     1201 ataagattga agaagcaact ggtggtgaaa aagtcattca aaccgtttgg ggagtgggct
     1261 ataaaattga cgcaagatag taaacgagca actgaaaaac gccgtcggat caatttgact
     1321 tcaaaagaaa tcagcgaatt gttagcggaa ggaatcatca cgattatcct tttgcttcta
     1381 ttaaatgttt ctattctggt tgttatcagt tcggtgatca acagttcacc ttctttgacg
     1441 aatgcgattt gggattcaaa gaatattttt gcagaacgat tgaacactga tttgttctgg
     1501 aacggccgga attttattat tccctttttc tttctattag atattggtgt tttgtattgg
     1561 cgcttgattc gaagatatcg ccaaatgcaa ttgcggcata tcatcagcga acttcactac
     1621 atcgcaaacg gaaattatga tcatcgtatc ccttttgaac tgagtggtga cttaagccga
     1681 gtggtaacaa gtatcaatgg attggtggat agtaccgtag cagcgattga agacgaacgt
     1741 aaaatcgaaa aatcaaaaga tgaattgatc acgaatgtca gtcatgatat ccgtacccca
     1801 ctcacgtcca tcattggata cctaggactg atagaagatg gccagtatca ctcagaagaa
     1861 gatcttttaa agtatacgca tactgcttat atcaaagcaa agcagatgaa atctttagtg
     1921 gacgacttat ttgaatacac taaggtacgc cagcctgctg tcccggtgaa tttttctgcc
     1981 tttgacatga tccagttgat cgaacagtta gcagctgatt ttgagttgga agcttctaaa
     2041 aaaaatatcc aaatacttgt tcaatcaaaa gtagactccc ttatcatgga tggcgatacc
     2101 gaaaaacttg tacgtgtctt caataatctt ttgacgaatg cgttgaaata cggaaaagga
     2161 gcaacgaaga tcgtaatcga agtagaacgt atcggttcag aagttgttgc aacagtcaaa
     2221 aataacggag caatgatccc acaacaggca atcgataatc tattcgaccg tttttatcgt
     2281 gtagaagaat ctcgttcaca agcaactgga ggaactggac ttggtttagc aatcgctcaa
     2341 agcattgtgg ctttgcacgg cggatacatt tatgcaaaat cagataagca atggacttct
     2401 ttcatcatgc accttccaat caagaaaaat gaaaaattac ctattgatac acaggaagtt
     2461 attgacgaaa gctgaaatat tcgctattct tattacaatt attagaataa atgaaaaaca
     2521 cagaacccag tacactttat tctttttcag agagctgatg gcaggtgtaa atcagcaaag
     2581 gataatttgg aagttccagt tcgtttacgt gccctttcga aataagaaag gtcggttcaa
     2641 cagccgttat gtgttcaaca agtgcagctg acgctgaact tgggtggtac cgcgaatttc
     2701 agactgattc gtcccaagaa taatgggatg gatcagtctt tttttatttt caaaaaggag
     2761 gaaatcatat gttagatgta aaaatgatgc gacaaaatat tgaagaagta caagaaaaat
     2821 tgatgactcg gggcgtaaaa aaagaaacat tagctgaatt catggaacta gatgaaaatc
     2881 gtcgtaagct actagtcaaa agtgaagaat taaaaaaata tcgaaatgat gtttctgctg
     2941 agatcgccca attgaagcgt gcaaaagaag atgcttctgc taaaatcgct gaaatgaaag
     3001 aagtcggtgg caacatcaaa gcattggata ctgaaattgc tgaagtggat gaaaaattga
     3061 aacaaattgc aactactttg ccaaaccttc cccatgattc tgtgccagtt ggaacagatg
     3121 aagaggacaa tgtagaaatc cgtcgttgga gcgaaccacg tacatttgct tttgaaccaa
     3181 aaccacactg ggaaattgca gaaaatcttg atattttaga ttttgaacgt ggcgcaaaag
     3241 tagccggtag ccgttttgtc tattacaaag gattaggtgc tcgattagaa cgtgctgtct
     3301 acaacttcat gcttgatcag catatttacg aacacggcta tactgaaatg atcactcctt
     3361 atatcgtcaa cagtaaagcg atgtttggta cgggacaatt tccaaaattc aaagaagatg
     3421 ttttccaatt ggagaatact gatctaacat taatcccaac agctgaagta ccattaacta
     3481 actattataa tgatgaaatt ttagatggtg cagatttacc tatttatttc actgcactaa
     3541 gccctgcttt tcgttctgaa gccggaagtg ctggtcggga tacacgagga ctgattcgtc
     3601 ttcaccaatt caataaagta gaaatggtca aattttcgga tgtagagcat tcttacgaag
     3661 aattggaaaa aatgacagct gatgcagaag atatcttgaa aaaattgcat ttgccttatc
     3721 gtgtgatggc cttatcaact ggagacatgg gcttttctgc agctaaaact tatgacttag
     3781 aagtatggat acctgcacaa gatacttatc gtgaaatcag ttcttgttct aactgtgaag
     3841 atttccaagc acgccgcgca atgatccgtt atcgcgataa tgatggaaaa atccactata
     3901 ctcatactct gaatggttca ggacttgcag ttggccgtac agtagctgcc attttggaaa
     3961 actaccaaaa tgaagatggt tctgtcacag ttccagaagc gctcgttcct tatatgggga
     4021 atctgaaagt tattaaataa agaggctgtg aaaaagccgc ttagtttcga gtattaagaa
     4081 aaattttgtg aaaatggctt tccacatttt tgcataaatt ggacttaata ccgagaaact
     4141 ggcttttgaa caccatttat cgagaggttg tgaaatcagc gaattaacct gagtattaga
     4201 gaaaaaaatg gaaaaatagt ccctcatatt ttaccgtttg ttgaactaat accgagggtc
     4261 agctgattga acaccgttta gtggagaggt cgtaagccta ccccccagct cctagttaca
     4321 aggacaaatt caaaaaacag ctcttcatat tttatgaatg ttgtacttgt gattcggagg
     4381 tggcaaggga acactgttta ttcaaaaaga ggctgggaca gaagcgttta actccaagaa
     4441 ataagaagaa attcacgaaa attgcttttc aaatttttgt gaatttcttc ttatttccga
     4501 aggagttgct tctacttccg ccgtttatac gtgtttagag cgtgagacaa aagtgttctt
     4561 tacttttgtc ccacgctctt taatgttttt aagattcaac tgaatagttt ggtgcttctt
     4621 ttgtgatttg tacatcatga gggtgtgatt cttttaatcc actgccgctc atttggataa
     4681 attgcgcttc atcacgtaat tgctgtagat tggctgcccc aacataaccc attcctgaac
     4741 gcaaaccgcc gatcatttgg aaaataatat ctgcaacact tcctttataa gcaacccgac
     4801 cttcgattcc ttctggtact aatttatttg cttcattgac actaccttgg aagtaacggt
     4861 cttttgaacc tttttccatc gcgcctaatg agcccatacc tcggtatgtt ttgaatcgac
     4921 gtccttggaa gatttcgaat tcacctggtg attcatctgt ccctgccaac atacttccta
     4981 gcatcactgc atgtccgccg gcagctaatg ctttgacgat gtcaccagaa tatttgatcc
     5041 ctccgtctgc aataatcgct ttgccatatt gacgagcaac tgatgctgca tcataaatag
     5101 ctgtgagctg aggtacacct acaccggcta ccacacgtgt cgtacaaatc gatcctggtc
     5161 cgataccaac ttttacgaca tctacacctg catcatataa agcttttgtc gcttcagccg
     5221 tcgcaacgtt tcctgcaatc aatgttgcat ctgcaaaagt tgaacggatt tcttgaattt
     5281 ttcggatcac acctgcacta tgtccatgag ctgtatcgat gatgattgca tctgcccctg
     5341 cttctaataa agcattcgca cgctcaaatg tatcgcttgt tacaccaacg gctgctgcta
     5401 caagcaatcg accatgttca tcttttgctg catttggaaa ttcgatcact ttctcgatgt
     5461 ctttgatcgt gatcaatccg cttaaacgac cttcgttatc tacgattggt aatttttcga
     5521 ttttatgttt ttgcaaaatc ttttctgcat cttttagaga ggtacctact ggggcagtca
     5581 ctaagttgtc ttttgtcatc acatcagcaa tcggcatgtg ataatctgtt acaaaacgca
     5641 tgtcgcggtt tgtaattatc cctaccaatt ttctgttttc aagtgtttca acgattggca
     5701 caccactgat acgatatttg ctcatcaatt cttctgcgtc tgctacaaga tgctgtggcg
     5761 ttaagaagaa cggatcaatg attacgccac tttcagaacg tttgactttt cttacttcat
     5821 ccgcttgctg gctgatcgtc atgtttttat ggatcacacc tagtccgcct tgtcttgcca
     5881 tagcaatggc catcttgctg tcggtaacag tatccatact tgcactgatg attggaatat
     5941 tcaacttaat atttttagct aattgtacgc tcatatccac ttcgtttggt aacacgtgac
     6001 tttctgctgg tatcaataag acatcatcaa aagtaaatcc ttttttcgtg aactttgttt
     6061 cccagttgga catttttagt accactcctc taatttagtt tatctattaa aataattgat
     6121 tttttcattt aagtcaatga tctacttgga atcaagttag ctttctcata gaataccaat
     6181 ataaagcgtt tacatctgtg tatattaaaa gagattgtaa aaaagctgtc ctgcatcaag
     6241 taataagaac agccaatcaa aaacagcttc tcatgttttt gaattggttg ggtttattac
     6301 cgcagatgca aacttttgaa caccgtttag tggagaggct gtgaaaaagc tgcttagttt
     6361 cgagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa attggactta
     6421 ataccgagaa actggctttt gaacaccgtt tatcgtaaaa agggactgtg acaagacttt
     6481 tgtcacagtc ccttactatt atttaactat ggataaacaa aatgaggttc tttactaccc
     6541 ctagatatct agcaaaatag ctgatctttt tgctagttta tcttcttggg acggataaac
     6601 ttccaataat cccgtatttt ttcttcaagt agtaacggat tgctaaagca gttgccccga
     6661 taatgataac tactactgga tctaatactg gattgataac tggtggcaat agtgttgtcg
     6721 cagaaaacag tacgacccaa agaagtgtcg ttccacctag gataagcaat gtcttaccca
     6781 ttcctggttt cttgcttcga tctgctccgg gatattcata ttgatagatg tatttgtaca
     6841 tcatataaaa gacccagcca ccgattgcag aacctagaac gagtgtagaa aagccataag
     6901 ctggcgttgt tccacgtgaa aacaatccgg taatcgcaat catgatactt aatagaccaa
     6961 gcaacagcaa cgtattatct aaccacatca ataaaggggt ggatgaattt tttacttctg
     7021 ctggcttatt gatgattgct tccgttcttt cagagactgt cccaaacaat tgacgggctg
     7081 ttcttccgcc tttttgttct gcaactaaag taggcagaat gtcatgaagg gcttttactt
     7141 tttcttcttc agacaaattc gctgcttcta aagatttttt caaatcaaaa atgtactgtt
     7201 gatttctttt ggttaatttt gtttctaaac tattgttttc ttttacgatt gctcgtaatt
     7261 gttctggttc cattatctaa tagtcctcct tagccgttcc ttttctcaac ggtcgtcaca
     7321 aaagacattc taccatattc tacagctagt ttgatagccc ttttacacat taaagcggaa
     7381 taacattacg tcgccgtctt gtacgacata atcttttcct tctaatcgaa cacgtccagc
     7441 ttcttttgct gcatgcatgt ttccgtaatg atccaagtct tcaaaagaga cagtttctgc
     7501 acggataaat ccgcgttcga aatctgaatg gatgattcct gctgcttgtg gtgctttgat
     7561 tccttttttg aatgtccaag cacgtacttc ttgttcccca gcagtaaagt aagtagctaa
     7621 gcctaacaaa tcataagcag cacggatcaa ctgatccaaa cctgattctt cgattcctaa
     7681 agcttctaaa aattccgctt tgtcttcttc atctaattct gcgatttctt cttctgcacg
     7741 agcactgacc acgatcactt ctgcattttc ttctgcagca aagttgcgca cttcttgtac
     7801 ataatgattc gcgtctgcat ctgctacgtc atcttctgag acattggcta cataaagaat
     7861 tggttttgtc gtcaataaaa atagtgattt aacgattttt tgttcttctt cagtaaattc
     7921 gatcgttcgg gcagattttc cttcttccag tacaggtttg attttatcca gtacagctag
     7981 ttctgccact gcatctttat ctttggtttt agcaactttt gctacacgag tatagcgttt
     8041 gttgatagat tctaagtctg ctaacactag ttccaagttg atcgtatcaa tatcttctag
     8101 tggatcaaca cgtccttcta cgtgagtaat attatcatca tcaaaacagc gaacaacgtg
     8161 acaaatagcg tctacttggc ggatatggct taagaattga tttcccaaac cttctccttt
     8221 acttgcgcct tttacgattc cagcaatatc tgtaaattca aaagtcgtcg gaacagtttt
     8281 tttcggatgt acaagttctg ttaagcgttg caaacgccag tcaggtactt ctaccatccc
     8341 tacgttgggg tcgatcgtcg caaagggata gtttgctgct tctgctcctg cttttgtgat
     8401 cgcattaaac aaagttgatt ttcctacgtt gggcaaccca acaattccag cagtcaatgc
     8461 cataaattta ttcactcttt ctctgcattt ttttcaatta ctttttttaa tttcttttca
     8521 aaatctcgac gggtcatcat gacgatatgt ccgcaattca tacatctgat tttgatatct
     8581 gcgcccattc gaatgacttc ccagcgattc gtctggcaag cgtgaggttt tttcatttca
     8641 acgatatcgt gcaagtcgta cataaaaata gcctcctttt tgtttattct tcatcaaatt
     8701 gaatattcaa gatatcaaga attcgagtca aatctttttg agaaagatat tcaatctcga
     8761 tcttaccttt cccatttttt tcttggatag ctacacttgt accaaattta tccatcaaac
     8821 gatcttcgct ttctcggata taatatggtt tttcctttgc tagccttggg atttttttct
     8881 tttcttttcc atcattttga tttaactcag aaacaagctg ttccagttgg cggacagtta
     8941 aattttcagt tactgcacga ttggctaatt tcaagatcaa tgttttattt tttaaaccta
     9001 acagcgtacg ggcttgtccc atagataaac gctgatcttg taccatttcc ttgacaagat
     9061 ccggtaaaga aagcaagcgc aaataattgg cgatataagg tctgctcttt cctaaacgac
     9121 tagctacttc tgcttgtgtt agtttcaggt ttttcatcag catctcatat gcttctgctt
     9181 cttctaacgg attcagatct tctcgctgca agttttccag cactgctact tgcatcatcg
     9241 cttcttcgtc aaagtctcta acgatagcag ggatcgtttc tttttcagct agtttggacg
     9301 ctcgaaaacg tcgctcccct gcaataatct catatccttt tactgcggaa cggcgtacga
     9361 tgatgggttg aaagacacct gattgttcaa tagaattagc tagttcctgc aaagatactt
     9421 cttcaaatgt tttacggggc tgatacggat ttggtcgcag ctcattcaat ggtagctgga
     9481 caacggtatc atcttttaca tccaccgtct ctaagttttc taaatcttga aacagtgcat
     9541 cgattcctcg gcctaatcct ttacttttct tcacgactta ccacttcctt tgccaatgct
     9601 tggtagactt cagctcctcg agaacgtgga tcgtagtcga tgatcggttt tccatgacta
     9661 ggtgcttctg ataaacgaat atttcttgga atgatcgttt cgtaaacttt ttcgcggaag
     9721 tatttacgta cttcttctac gacttcattt cccagatttg ttcgtgcatc atacattgtc
     9781 agtaagactc cctcgatctc taattctgga ttaaagtgtt tttggactaa acgaaccgta
     9841 ttcagtaatt ggcttaatcc ttccaatgcg taatactcac actgaacagg tatcaaaatc
     9901 gaatcacttg ctgtaaatga attgattgtc agatggccaa gagaaggcgg gcaatcgatc
     9961 aaaatataat catactgact gcttacttct gctaaagatc cttttaatct tgattctctt
    10021 gccatcattg acgtcaattc gatctctgct cctgctaatt ggagagtcgc aggcacaata
    10081 ctcaaatttt cgtgttcagt gatcaaagtt gcctcatcaa ttggcaattc attgactaga
    10141 acatcataga tgtctcttgt cacatcaggt tttctgatcc cgacaccact ggtagcattt
    10201 ccttgtgcat ccatatccac tagcaatact tttttgccaa ggctagcaag acaagcacct
    10261 agattcaccg tcgtcgttgt tttgccgacg ccaccttttt ggttcgcaac agatattatt
    10321 tgtgccatct cttctcctcc tatttgattg gctgcttatt aggcagtccg ggctttcttg
    10381 ggtatttctt tggggttggt ttcttttttt gaataacgat gatatgccgt tgatcagaga
    10441 cattcggcaa ggtaaccgcc tcttctttga ggaatttacc acctaataaa gcaatcgctt
    10501 gttttgcttc tgtcaattcc tcttcacttt ttgctgcttt caatgcataa aaatagcctt
    10561 cttgtttagc caagggcaaa cacaattccg ctaacacatt caaacgagca accgcacgag
    10621 cagtgacaag gtcaaatgat gcacgaaagt gaggattttg cccaaatgtt tctgcccggt
    10681 catgatataa ggaagcatcg attcctaata atcgggttaa ctcagataag aaggttattc
    10741 gtttattcag agaatcaacg atcgttatct gcagttctgg aaaggcaatt tttaaaggta
    10801 tgctaggaaa ccctgccccc gaaccaacat cgcataaaga taaaggttgc gtaaagtcta
    10861 cttcaaatgc taatgtaagt gaatcgtaaa aatgcttcag atatacgtct ttttcatccg
    10921 taatagctgt aaggttcatc ttttcgttcc attcgactaa tgtccgataa taaatagcga
    10981 attgattcat ttgatgatcg gtaagctcta tccctttttc tgccaaggat tggcgaaatt
    11041 cttctggtat cataaatatt cctttcattg tgaaacagca attgtttcac agattctctc
    11101 cctactttaa cgcaaaatct ctagacatgc aatccttcca tatggattct taaaaatttg
    11161 aaaagtaaga aaagccgata gactttttct ttctagtatg actcataaaa tatacacaga
    11221 agagagaact agctttgagc aatttctgca aaaactagtt ctctcttcta tttattatta
    11281 cgtattacgc tggttcactg actttcgcaa tcttcccttg ttcgatatag accattagga
    11341 tacttaggtc tgcagggttt acgccactga ttcggctagc ttgggcaatt gtttctggtt
    11401 ggattttctg caatttttgt tttgcttctg ttgctaatcc gttgattgct gcgtagtcga
    11461 tgttttctgg aatctttttc gcttccatgc gttttaattt ttctactttt tccatggctt
    11521 ttttgatgta gccttcatat ttgatttgga tttctacttg ttcgatttct tttcgtgtca
    11581 attcttcatt ttctggaata aaccgtagaa gttctgcata agtaatctca ggacgtctta
    11641 aaaagtcgct tgctagtaca ccatctttta atggtgccgc attaatcgtt gctaaaaagt
    11701 cttggacttc ttctgttggt ttgattcgta cattcatcaa acgtttgatt tcttgttcaa
    11761 ctgcttcttt tttcttcaaa taattcttgt attgatcttc ttttactaag cctacacggt
    11821 gacccatttc tgttaatcgc aaatctgcat tatcatgacg taaaagcaga cggtattcag
    11881 cacgtgaagt cagcaaacga taaggttcgt tcgttccttt tgttaccaaa tcatcgatca
    11941 tgacaccgat gtaaccgtca cttcgtttga tcactaaagg ttcttttccc tgaactttca
    12001 aagctgcatt gataccggca atcagccctt gtccagccgc ttcttcataa ccgcttgtac
    12061 catttgtttg tccagctgta taaagatttt caacgacttt agtttctaat gtcggacgga
    12121 gctggtgtgg ctctactacg tcatattcga tagcatatcc tgtacgcatc atttctactt
    12181 tttctaatcc agcaatagaa tgaagcatgt tgacttgaac atcctctggt aaagaagtag
    12241 ataaaccttg aacatagact tcttccgtat ttaagccttc tggttccaaa aataattgat
    12301 gacgtggttt gtctgcaaaa cgcacgatct tatcttcgat ggatggacaa tagcgagcgc
    12361 ctaccccttc aacgatccct gtaaacattg gggcacgatg taagttgtct cgaataattt
    12421 ggtgagtcgt ctcacctgta taggtcagcc agcaagattc ctgatctagt ttataggcac
    12481 tatctggtgt gctgaagcta aaatgattcg gctctttgtc accaggctgc tcttctgtaa
    12541 cagaatagtc gatcgtactt gattttactc gaggcggtgt tcctgttttg aaccgttcga
    12601 tagcaaagcc aagttctttt agatgattcg ctagtccaat agaaggttga gagttattcg
    12661 gacctgatga atatttcaat tcaccgataa tgatttctcc gcgaagtgct gttccggctg
    12721 taataacaac tgctttggcc cggtagctcg ctcctgtgga cgtcactact ccatgacaaa
    12781 caccatcttc gacgatcagt tcttcgacga ttccttgtct tagcgtcaaa tttggttctt
    12841 tttcgatcgt atgtttcatt tctgttgaat aagcatgttt gtctgcttgt gctcgcaatg
    12901 ctcggacagc tgggcctttt cctgtgttca acatacgcat ttggatatag gttttatcga
    12961 tattcttacc catttctccg cctaacgcat cgatttctcg aacgacaacg cctttagctg
    13021 gtccgcctac agaaggatta catggcatga atgctaccat gtccaaattt attgtcagta
    13081 ataatgtttt tgatcccata cgtgcagcag caagtgcagc ttctgaaccg gcgtgtcctg
    13141 cgccaacaac gatcacgtca tacgcatctg cttcataatg ttccaatcgt ttgttcctcc
    13201 ttttattttc ctaaacaaaa ttgactgaat aattgagtga ttagctcatc ttgtacactg
    13261 tcgccgacaa cttctcctag atagtcccag caacgcgtca tatctatttg aacgagatcc
    13321 acaggcattc ctgaatcaat acctgcaatg acttcttcaa gtgagaggga agctttttct
    13381 aacaacgcga tatggcgggt attagaaaca taagtagcgt ctctttctcc tgtttctccg
    13441 ctgaagaata aatcagaaat agctgattcc aaacgatcta gtccgtcgtt tttagctacg
    13501 gaaattgaaa aaactggttc attttcgatc aatttcttca atttttcttg ttccagctgt
    13561 gctggcaaat ctgttttatt tagaagaatg atccgtttca atccacttgt cgcttccaat
    13621 aattgttcat cttctgctgt cagtggttcg ctttggttta gaacaagtaa gatcaagtct
    13681 gcttctgcta atgcttttcg gcttctttcg accccgatcc gttccaccac atcttctgtt
    13741 tcccgaattc cagctgtatc gattagcttg agcggtaccc cccgaacatt gacatactct
    13801 tcgattacat ctcgagtcgt tcctgcaata tccgtaacga ttgctttttc ttctcttaac
    13861 aaatggttta acaaacttga tttgccgaca tttggccggc cgatgattgc cgtacttaac
    13921 ccttcccgca gaaccttccc ttgcttggat gtagcaagta gcgcttggat tctttgttgg
    13981 atcatctgcg ctttttcaag caataacttc gtagtcaatt cttcgacatc atcatattct
    14041 ggataatcaa tatttacttc tacttgtgct aatgtctcta aaatctcttg tctcaatgag
    14101 cggatcaaag cagacagatt gccgtctaac tgattaagag ctagtcccat tgctttgtct
    14161 gtttttgccc gaatcaaatc catcacagct tctgcctgtg aaaggtcaac acgaccattc
    14221 aagaatgctc gttttgtgaa ttcgcctggc tcagctaatc ttgcaccttc tcttaacaat
    14281 agctgcaaaa tttgattgac tacaacgatc ccaccgtgac aattgatttc tactacatct
    14341 tcccgcgtaa atgtctttgg tgctctcatc acggagacca tgacttcatc gacaagctga
    14401 ttgctcttgg gatcaacgat atgtccataa tggatcgtat gacttggtac ttctgataat
    14461 cgtttgtttc cagattgata aactttatct gctagttgca cagcttgatc gccacttaaa
    14521 cgaacgatac tgattgcacc ttctccaggt ggggtagaaa ttgccgcaat cgtatcaaat
    14581 tctaaagtaa tttcagccat tttttcactt cctttttcat tttgcgcaca aaaaaagtgc
    14641 ccactccacc ctttatgcac aataaacggt gaacttcgca ctttgactgc ttgttcttga
    14701 ttaaattctg gtcatagatt atcacacttg ctataaggaa tcaagtgttt aaaacaatta
    14761 gatgaagttg agcctgttct gcttccatta ccttatattt aattgtcctt ggatgtctct
    14821 ttgcgttctt tggtttattc gaagagaaga tactgtataa acaaggcttt atctttctta
    14881 aaaaaaacgg tacactaaat ggtagcgcta tctaaactat acacttttga aaggagaaaa
    14941 tatgtgagcc cgttcagatc tgctgcattg gaagaagaaa gtgaccctcg gatcaacgat
    15001 gtcgttttat ctacagggaa tatcaaccag aaatatatcg tccgcgatat cgtttttgtt
    15061 gtggaatgct tagaagctga tttgtttgac ccatcttttc ccaaagatga ccttttctta
    15121 acaacgaaga ggaaattaaa gacgaaggta cttgaatatg gtgcgaatgc agtaatcaac
    15181 tgtcattttg agtatcagca aaaaaaacgc gacggaaagt attttctgga actttttgct
    15241 tatggaacag tgatccaatt tatccaaaca acgattggtt aaatgaaaca gacaaaaaaa
    15301 gcggatcaaa actgaagaca gtttcgatcc gcttttttct atcaaagtta gtgactactt
    15361 catcgtatac tctgataaga atgatttcta gtaaaatata cttggtttga aaaactttgt
    15421 caacgtgatt tattcaaaaa gtaatggaat caataatatt ttttagcagg ttctactact
    15481 aaataacgat aaggttcctc accttccgaa tgtgttttta catgttcatc tttgcttaat
    15541 gcagcatgga tttgtttacg ttcaaatgca ggcattggtt ctaagaaaac aggctgccct
    15601 gtccgtttta ctttttcagc cgtgcgatca gctaaacgct gaatgatctc ttggcgcttt
    15661 tcacggtaat tgcctacatt gactactaca gacagcttat ttcttgctaa acgatggatg
    15721 aagacttgtg ccagatattg caatgcattc agtgtttttc catgtttccc aatcagtatg
    15781 ccttgctttt gtgtttctaa atggaaaaca accatattat ttgattctct ttccattttg
    15841 acaagagctg gagcatcaag ctgttttgaa atttcagtaa gataaactgc caattccgtt
    15901 aaagctgctt catcacctag ctcacttact tcttctgttt tttcttcaag aatcgtttcg
    15961 ttgactacct cgatctcagc tgttggttct tcttccaaaa tttcttctac agtctgttct
    16021 actgtttcag aaactttttc gctgatactt ggctggatcg aaagccgtgc attctttcga
    16081 cccattccta ggaaaccttt ttttccttct tccagtattt cgatttcgac ttggtctttg
    16141 gccaaaccca aatcagccag acctagttgg acagcttcgt ctattgttgc agcttcataa
    16201 atcggcatta gcattacctc ccctattatt tccgtttttt cttaggattc attgctttct
    16261 taagagcacg ttctctttcg cggattctcc gtgcttcttc ttcacgctct tttcgaattt
    16321 tgaaagggtt gttgatcagc agcgtttgta caacttggaa ggcattggaa actacccagt
    16381 ataatgaaag accgctggct aaattgacac ccatcactaa aatcattaca ggcatcacga
    16441 aattcatgat cttcattgat gcattcgatt caatttgact catgctagat aaatacgtac
    16501 ttgcaaaagt aaagattgct gctaaaatcg gcaaaataaa tgttggatcc gcgttcccta
    16561 agttcaacca taagaaatgc ccttgtttca actctggtac acgtgaaata gattgccata
    16621 acgccatcaa gattggcatt tgtactaata aaggaagaca accagcatat ggattgacgc
    16681 cattttctgc atataaacgc tgctgctctt ctcttaactt actttgtgtt tccggatctc
    16741 ttgaactata ttgttgctgc agtgctttta atttcggttg taattcttgc gttttacgca
    16801 tgctctttgt ttggaaatgc atcaacggca ataggatcac gcggatgatc agtgtaaata
    16861 aaatgatccc tatccctgca ttaccaaatg acaacgcctt gatcgcctca gcaaagtaat
    16921 aaacaatata acgatcccaa attcctgtac tctgtgcgct gacttcccca gttccgcaac
    16981 cggataagac gatgaccaaa ctaacgatac cggccatcaa aagtaaacgt ttgtatttct
    17041 tcacttactc ttttcctctc tgtcgattat ttttgcaagt tttaagacgt ggactaaatt
    17101 ggagcgaacc tcatcataag tcaacccctt cacacttggt ctagcaatca caataaaatc
    17161 caactccgga tcaatttctt cttttataga ataaactgct gctcggattt ttcgtttgac
    17221 ttcattgcgg tttaccgcgt tgccgacctt ttttcctaca gataaaccaa ctcgaaaatg
    17281 aggctgtccc ggtttttcca aacggtaaac gacgaatttg cggttagcaa aagaatctcc
    17341 cgtatggaac acggcttgaa actctcgttc tttttttact cgatacgctt ttctcattag
    17401 ttttctttcc ttttctatct ctcacttacg ctcataatca taccataatt acgattgtcc
    17461 tttcatcatt tccctaaaaa agagtataaa aaaaaccact gaaaccccag tggcctaagc
    17521 tgaaagaact tttcttcctt tgcgacgacg gctagctaac acacgacgtc catttttagt
    17581 actcatacgt ttacggaatc cgtgaacttt ttgacgttta cgtttatttg gttggtatgt
    17641 tcttttcata tattccacct ccaaaatgtt cttatctatt ttataaacac agacatactt
    17701 tgatagtata acgacaaagt agtgattttg tcaatataaa atagaagatc gattgttaaa
    17761 acttatccac aagattattt ttttctatgg gaatcctatg tggaaaaagt tatgcacagg
    17821 gattatccac agataataaa aggctggttt tacacagaaa acacacgtgt aaaaaaagta
    17881 atccacaatc aggcgatttc ctgtgtattc cgtctcctaa ctattggtat atctgtattt
    17941 tttatccaca gacgcaagag gaaatctatt ggtttttacg agttttccac ctgtgcatta
    18001 gtggtgtgga aaactttttt tgacaactgt gtttttctct tttttctttt tgtcgattgt
    18061 gtggataatt gtaattaaaa aatttttttc ttcggtttct gtggaaaact attgaaaaaa
    18121 atgctaaatt agtactagtt attcactttt taatgaagga ggaggcctta tatggtatcc
    18181 ctcgatgctt tatggaatga attaaaagca acataccaaa aggatttatc gcctgctagc
    18241 tataatacat ggatcgaaac agcgaaccct cgaacccttg atcaaaacca gctcgttgtt
    18301 gaggttccta gcaagattca taaagaatat tgggaaaaaa acctggcgac aaaaatcgtt
    18361 gaagtcggtt atatgttatc aggaaatgag attatccctc gctttattac tggcgaagaa
    18421 gcagagcaag aagaagttat agaagaaaaa aatccaaaag ttgtggcacc tagtccgcta
    18481 aaaaaagcca tgctgaaccc taaatatacc tttgatacgt ttgtcattgg taaaggaaac
    18541 cagatggctc atgcagcagc gctcgttgta gcagaagatc caggttctat ttataacccg
    18601 ctcttctttt atggaggcgt gggacttggt aagactcact tgatgcatgc aattggtcat
    18661 caaatgctgc aaagtcagcc taatgccaaa gtaaaatatg taagcagtga aacttttgct
    18721 aatgatttca tcaattcgat ccaaaacaaa acagcagaag agttccgtca agaatatcga
    18781 aatgttgatt tactgctagt ggatgacatt caattttttg cagaaaaaga agctacgcaa
    18841 gaagagtttt tccatacttt caatgccttg tacaatgaag ggaaacaaat cgtactaacc
    18901 agtgatcggt tgccaaacga gattccgaaa ctgcaagaac gccttgtttc tcgctttgca
    18961 tgggggctgt ctgttgatat tacaccgccg gatcttgaaa caagaacagc gattttacgt
    19021 aaaaaagcgg cggctgaacg tctagagatc ccagatgaca cactaagcta catcgcagga
    19081 caaattgatt ctaacattcg tgaattagaa ggtgcgcttg ttcgagtgca agcttttgct
    19141 gcgatgaata gtgaagatat ttcgactagt ctagctgcag acgcactgaa aacgttaaaa
    19201 tcaggaaaag gacatcctca attgtcaatc ctgcagatcc aagaagaagt ggcaaagtat
    19261 tatcacatcc aattgaaaga tctcaaagga aagaaacgcg tcaaatctat tgttgttcct
    19321 agacaaatcg ctatgtattt ggcacgtgag ctcacagata attctttacc gaaaatcgga
    19381 gctgaattcg gtggcaagga tcatacaacg gttatccatg ctcatgaaaa aatccagcag
    19441 ctgatggatt ctagtgtcag tatgcaaaat gaagtatcag aaatcaaaaa ctttttacta
    19501 aattaatttg tcgattctat caactgaaaa tagcatgtgg ataaaagaaa aaaacgttca
    19561 aaagttatac acactttttc cacagctgtt ttttcgtttc tctcttacgt tttttgagtt
    19621 ttccacagaa tcaacatgcc ctattacttt tattattctt tttaactaat aataaataaa
    19681 caaacattca aaggagatac atcatgaaag ttactttaaa ccgagctagc tttatgcagg
    19741 aattgcaaac tgttcaacga gctatttcaa gcaaaaccac gatccctatt ttgacaggtg
    19801 taaaaatcac actgacacaa gaaggtttga ctttgacggg gagcaacgct gatatatcaa
    19861 ttgaaacttt tttgtctgtt gaaaacgaaa aagcaaatat gcaaatcgaa tctactggtt
    19921 ccattgtttt acaagcacgt ttctttagcg aaatcattcg gagacttcct gaagaaacat
    19981 ttactttaga agttttagaa aataaacaag tagcgatcac ttctggaaaa gcgaatttta
    20041 tcgtaaatgg attagatgca gataactatc ctcatcttcc tgttgtcgaa agccataacc
    20101 agatgaaatt acctgtacac gtattgacta aactaatcaa cgaaacagtt tttgctgtct
    20161 cacaacatga gagtcgtcca atcttgacag gtgtgcattt cattttatct gataattctt
    20221 tattagctgt agctactgat tctcaccgtc taagtcaacg cgtgattcca gtagaacaag
    20281 cggctgatca ttttgatatt gttattcctg gaaaaagttt gatcgaatta tctcgctcat
    20341 taacaaatga agaagaaatc gtcgaaatca gcattatgga aaaccaagtg ttgttcaaaa
    20401 cagaaacgat gtatttctat tcccgtttgc tagaaggaaa ctatccagat accaatcgtt
    20461 tgattccttc aagttttaat acagaggttg aattttctgt tcctagcttt ttagcggcga
    20521 tcgaacgggc ttctttactt tctcatgaag gccgtaacaa catcgttcgt ttatctattc
    20581 gtccagatgc ggttgtttta tacggaaatt cgccagaaat cgggaaagta gaagaaagct
    20641 tgagttatac agcaagcagt ggcgatccat tagatatttc tttcaatccg gattatatga
    20701 aagcagcgtt gcgtgcattt ggagatatga gcatcaaagt gaaatttatc tctgctattc
    20761 gtccatttac attggaacca acggaagatg gcgttcaatt tattcagctg attactcctg
    20821 tacgtacaaa ttaacaatga ttttcaatgt tcttgaaaat ttaattgaaa agaaatagaa
    20881 aaaataaaat tttaaacaga aggccctaaa atctctttta aggctttctg tttatttttg
    20941 tttaaatcac cttttttaga ttaaatcgtc ttatatggaa aaatatatgg ataaatttgt
    21001 tttattgcga aaaaaaaggt ataatagagt atacttttaa taatgaaaat gagggatggg
    21061 cattgaaaca acaagtcatt ctaaatacgg aattcatgac gttgggccaa atgttgaaag
    21121 aagtcacagt tatcggtagt ggcggacaag caaaatggta tttggcagaa aacactgtgt
    21181 tagtcgatgg cgaaccagaa aatcgtcgtg gacgcaaact ttatcccggc atgatggttg
    21241 aagttccaga agtaggaact ttttttatgg taaaaaaagg aaacgacgat aatgaggctg
    21301 aataagctgt atttaaaaaa ttatcgaaat tacgaagagc tgaacatcga attttccaag
    21361 aacctcgtca tctttttagg agaaaatgcg caaggaaaaa cgaatctttt ggaaagtatc
    21421 tacgtattag ctatgacgcg aagtcatcgg acaagtagtg agcaggaatt gattggctgg
    21481 actgacgatc aggcgatgat tcaaggagag atcacaaaag gttcatccac tcttccatta
    21541 gaaatcttat tatctaaaaa aggccgaaaa accaagatca accatatcga acagaaaaag
    21601 ttaagtactt acgtgggaca aatgaatgtc attttatttg caccagaaga tctttcttta
    21661 gtaaaaggca gcccgcagac caggcggaag tttttagata tggaattagg ccagatcaat
    21721 ccaatctatc tttatgatct tgtccaatac caagcaattt tgaaacaaag aaaccagtac
    21781 ttgaagcaat tgaatgaaaa aaaacaaact gatttgcttt atttggatat tttgtccgaa
    21841 caactagcag cgtttggcag caaagtattg aaagcaagag cacagttcgt caagcgattg
    21901 gagttttggg ctaattcttt gcatcagcaa atcacccatc aaaaagagca gctggaaatc
    21961 gagtatctca ccgcagtcga ttctttagaa acacatacgc aagagcaaat ccaagaacaa
    22021 tttctcgcac ttttgaacca aaataaaaag aaagaccttt ttcggggaac aacgacagtt
    22081 ggtcctcatc gtgacgatct ttcctttttt attaatcaga aaaatgtgca gacttatgga
    22141 tcgcaaggac agcaaagaac aactgcacta agtgtcaaat tagcagaaat cgatttgatt
    22201 aaagaagaga caggggaata tccaatcctt ttgttagatg atgtgatgag cgagctggat
    22261 gataatcgtc aactccatct gctagaaaca atcgaaggaa aagttcaaac gttcttaaca
    22321 acaacgacac ttgaccatgt aaaaaataaa atgactgttg aacctgagat tttttatgtt
    22381 caacaaggga aactaaaagg agaagaaact gaatgacaga agaaagaagt ttagtagaac
    22441 gcgctaaaga gtatgatgcc agtcagattc aagtacttga aggacttgaa gccgttcgta
    22501 aacgacctgg catgtatatc ggttcgacta gttctgaggg tctgcatcac cttgtttggg
    22561 aaattgtcga taattcgatc gatgaagtat tagcaggttt tgcaacgaaa atccatgtga
    22621 ttattgaaaa agacaacagt atcaccgtta ttgacgacgg tcgggggata cctgtagata
    22681 tccaggcaaa aactggacgt cctgcagttg aaacggtctt tactgtttta catgctggag
    22741 gaaaattcgg cggtggtggg tacaaagttt ctggtggact tcacggagta ggatcttctg
    22801 ttgttaacgc gctttctaca cagcttgatg tcaaagtata caaagaagga aatgtgtact
    22861 atcaagaata tcgtcgtgga gctgtagtgg acgacttaaa gattatcgaa caaacggatc
    22921 gccatggaac aacggttcac tttacgccag accctgaaat ttttacagag acaactgagt
    22981 ttgatttttc aaaattggct acccggatcc gtgaacttgc tttcttgaac cgcgggatgc
    23041 gtatatcgat tgaagacaaa cgagaagaag aaccagtcat caatgaatac cattatgacg
    23101 gcgggattaa aagttatgtc gaatacttga atgcaaacaa aaccgtgatt tttccagaac
    23161 ctgtatattt ggaaggtgaa cagcaagata tcgcagttga agtctctatg caatatacag
    23221 acggctacca ttccaatatc atgagttttg ccaacaatat ccatacgtat gaaggcggaa
    23281 cacatgaatc aggattcaag actgctttaa ctcgtgtgat aaatgattat gcccgaaaac
    23341 aaaagctgat gaaagaaaat gacgacaact tgaccggtga agacgtgcga gaaggattga
    23401 cagctgttat ttctatcaag catcctgacc ctcaattcga aggacaaaca aaaacaaaat
    23461 tgggcaattc cgaagtacgg acagtcactg atcggttatt tagcgaacat ttcatgaaat
    23521 tcttgctgga aaacccaagt gtgggccgtc aaattgttga aaaaggatta ctagcttcca
    23581 gagctcgtct agcagcgaaa cgtgcgcgtg aagtaacgag acgtaaaggt gctttagaga
    23641 ttagtaatct gccaggaaaa ttagcggact gctctagcaa cgatcctgaa aaatgcgaat
    23701 tgttcatcgt cgaaggagat tcggccggcg gatcagctaa gcaaggtcgt aatcgagagt
    23761 ttcaagccat cttgcctatt cgtgggaaaa tcttgaatgt tgaaaaagca agcatggaca
    23821 agatcttggc aaatgaagag atccggtctt tatttactgc tatgggtaca gggtttggag
    23881 aagattttga tgtatcgaaa gctcgctacc acaaattagt tatcatgact gatgccgatg
    23941 tcgacggcgc gcatatccgt acattgctgc tgactctttt ttatcgttac atgcgtccta
    24001 ttgttgaagc tggatacgtc tatattgctc agccaccttt atacggggta aaacaaggga
    24061 agaatattac gtatgtacaa ccaggaaaaa atgcagaaga agagttgaaa caagtcgttg
    24121 cttcacttcc tgcctcacca aaaccaagcg ttcagcgtta taaaggactt ggagaaatgg
    24181 atgatcatca actatgggaa acaactatgg acccaagcaa tcggatgatg gcccgcgtca
    24241 gtgtggacga tgctattgca gcagatcaga tatttgaaat gttgatgggg gatcgagtag
    24301 aaccccgtcg tgcattcatc gaggaaaatg cacactatgt gaagaactta gatatttaat
    24361 catgaaggag gaaaaagaat gagtgaagaa atcagagaaa acatccatga tgtcaatcta
    24421 accagcgaaa tgaaagactc cttcatcgat tatgcgatga gtgttattgt cgcacgtgca
    24481 cttcctgatg tcagagatgg tctgaaaccc gttcatcgcc gtattttata cggcatgaac
    24541 gaattaggtg taacaccgga taaagcccat aaaaaatctg ccagaatcgt cggagatgtc
    24601 atgggtaaat accatcctca tggagactat gcgatctatg agtcaatggt acggatggca
    24661 cagccattca gctaccgtta tatgctagtg gatggtcatg gaaactttgg ttctgttgac
    24721 ggtgacggtg ccgccgctat gcgttatacc gaagcacgga tgagcaaaat cgctacagaa
    24781 atgctgcgtg atatcaataa gaacactgtt gatttccaaa gcaactatga tgatacagaa
    24841 aaagaaccag ttgtactacc tgctcgcttc ccgaatcttt tagtgaacgg aacaacgggg
    24901 atcgccgtag gtatggccac gaatatccca cctcataact tgtctgaggt agtagcggct
    24961 atcgatttgc tgatggataa tcctgatgtg acgaccaatg aattgatgga agtattacct
    25021 ggaccagatt ttccaacagg tggtttggtt atgggaaaat caggaatccg aagagcgtat
    25081 gaaacaggta agggttctat taccgtacgt gcaaaagttg agattactga gatgccaaat
    25141 ggtaaagaac ggatcatcgt gacagaattg ccttatatgg tcaataaagc aaaattgatc
    25201 gaacggattt ctgaactgca cagagacaag cgtatcgaag ggatcacaga tttgcgggac
    25261 gaatcttccc gtgaaggtat gcggatcgtc attgacatcc gtcgtgacgc cagcgcatct
    25321 gttatcttga ataacttata taaattgaca tccttgcaaa attcatttgg attcaatatg
    25381 ttggcgatcg aaaaaggtgt gccgaaagtt ttgagcttga aacaaattct tgaaaactat
    25441 atcgaacatc agcgcgaagt catcacaaga cggacagtct ttgaaaaaga aaaagcagaa
    25501 gcaagggcac acattttaga aggtttgcgt attgctttgg atcatatcga tgaaatcatt
    25561 gcgattatcc gcgggtctaa atcagatgac gaagcaaaag cgacgatgat cgaacggttc
    25621 gatttatccg atcgccagtc tcaagctatt ttagatatgc gtttacgccg attgaccggt
    25681 ttggaacggg aaaaaatcga aaatgaatac caagaactct taaaactgat tgaagattta
    25741 actgacattc tggcaagacc agaacgtgtt acagaaatca tcaagaatga attagcagac
    25801 ttgaatcaac gatttggtga taaacgacgc acagaattac tagtcggtga ggtcttaagt
    25861 ctggaagatg aagatttaat cgaagaagaa gaaatcgtta ttacattgac aaacaatggt
    25921 tacatcaaac gtttggcaaa taatgaattc agagctcaac gaaggggtgg acgtggtgtc
    25981 caaggaatgg gtatccacaa tgacgacttc gtcaaaaatc tggtttcttg ttctacccat
    26041 gatacattgt tattctttac gaataacgga aaagtgtatc gggcaaaagg atacgaaatt
    26101 ccagaatacg ggcgcacagc caaaggaata ccaattatca atttactagg tatcgattcg
    26161 agtgaaagca tccaggcaat cattgcggtg acgggagaag cagaagaagg ccactatctc
    26221 ttctttacga cgcgtcaagg tacagtaaaa cgtacttctg tcactgcttt tgctaacatc
    26281 cgaagtaatg gtctgatcgc aattggtttg aaagaagacg atgagctcgt caatgtgcta
    26341 ctgacagatg gtcaatctaa tatcattatt ggaacacata acggctactc tgtcactttc
    26401 aaagaagaag cagtccgcga tatgggacgt acagcttccg gggttagagg aatacgcctg
    26461 cgtgaggaag attatgtcgt tggggcagcc ttaatgaagg aagatcaaga agtattagtg
    26521 atcacagaaa aaggttacgg aaaacggaca aaagtatcgg aatatccagt gaaaggccgc
    26581 ggaggtaaag ggatcaaaac ggcgaacatc actgaaaaaa atggtccact agctggattg
    26641 accactgttt ctggagatga agacatcatg ctgattacag ataaaggtgt cattatccgt
    26701 ttcaacgtat cgactgtttc tcaaactgga cgttcaacat taggcgtacg tttgatgaag
    26761 atggaagctg atacgaaagt tgtcacgatg gcagcagtcg aaccagaagc tgcagaacaa
    26821 acagttgaag aacaaggatc agaagaatag ccatcaagac taattgtgta agctagcaag
    26881 aatgaccgat taaaaaatct ggttattcct gctggctttt ttgattagtt gaatttctct
    26941 taaaatattc gaaactgttc attctgtatt gcttttgacc aaaatagagt gtataatcag
    27001 agcttgtgag tagtctaatt aattagataa ctctccttgc tcttaggaac ctaagagccg
    27061 aaagtccaca gggaggtgaa tatcaatgga aaacacgaaa tatgaaatca tgtacattat
    27121 tcgtccaaac attgatgaag aagcaaaaac tgctttaatc gaacgtttcg atgcaatctt
    27181 gaaagataac ggagcagaag ttatcgaatc aaaagattgg gaaaaacgcc gtttcgctta
    27241 tgaaatgaac ggttaccgtg aaggtatcta tcacatcgtt aaagtatctt ctccatcatc
    27301 agcaaatgct gtcaatgaat ttgaccgtct tgctaaaatc aacgacgata tcattcgtca
    27361 catgattgtt aaagaagaag cttaattgtt gtttcatgtg aaacaataga aaacgatgag
    27421 aggagatgac tattttgatt aataatgttg tattagttgg acgtctgacg aaagatccag
    27481 atttgcgtta cacagcaagt ggtacggcag tcgcaacatt tactttggct gtgaatcgta
    27541 attttacaaa ccaaagcggc aatcgcgagg ctgattttat caactgtgtg atttggcgca
    27601 aatcagcaga aactttagca aactatgctc gtaaaggaac acttttaggt gttactggac
    27661 gtatccaaac tcgttcttat gataatcagc aaggacaacg tgtatacgtg acagaagtag
    27721 tagctgacaa cttccaattg ctggaaagtc gttctgcatc tgaaaatcgc caacaaagcg
    27781 gcggattcca aagttctggt caatcagctg gcggatttgg cggaaacaat aactctaatc
    27841 aaacatctca atcatccaac ggtatgcctg atttcgaccg tgatacttct gacccattcg
    27901 gctcaagttc aacgatcgac atttcagacg atgatttacc attctaaacg aatttgatag
    27961 gagggaaata gaatggcaca acaaagaaga ggcggacgta aacgtcgtaa agtagactac
    28021 attgctgcta accatattga atatatcgat tataaagata tcgaattact aaaaagattt
    28081 atttcagaac gcggtaaaat cttgccacgt cgtgtaactg gtacaggcgc taaaaaccaa
    28141 cgtaaaatta ctgttgcaat caaacgcgct cgtatcatgg gcttgctacc tttcgttagc
    28201 gaagactaat aaatcctagg aattgaatga ctgatccttt tgatcagcta ttcaattcct
    28261 ttttttctgt aaaaataacc tctattcgtt tagtcgctcg aatgacgaaa gttatggtaa
    28321 aatcaaatta gtcatatatg ggaggaaaat tatggaaaat aaaaacaatc atttatcccc
    28381 gaaacctctc ttccttatcc tgtttatatt aatcgaagta ttggtagtct taattttacc
    28441 atttcgtttt gttagcgctt cggtcattct attgatgaat ttatggggtg ttcgctattg
    28501 gttgaaaatg aaacgttggc ttattttaaa taaacgtgaa aaaatccagc aggcttcgac
    28561 tgtagctgaa accaatctag cttatgtctc agaagtcatg cctgtaggag tgattgctta
    28621 tcagccatca agccaaaaaa tcgaatggct caatccatta gcattgaaaa tcaaagatca
    28681 aagtggatta acagaggaag aattgctaga tacttttttt actgcaaaag aaaaaaagaa
    28741 aaaccaagtc tatctagcag ataaaacatt tcagtttgat atggatacag taaagggtgt
    28801 tgtttatttt aatgatatta cccaaaacca gttgttaaaa ctccatcaga cgaacagtca
    28861 gccggtgatt ggtattttat ctattgataa ctatgcagat gccattgata aattggatga
    28921 caaagagatt tcttatttga actctttcgt cactacacta gtttctgact ggatgaatga
    28981 ataccatgtc tttttcaaaa gaatcaacgc agaacggttt ttcttcgtag cacagatgat
    29041 cgatctgaaa aagatgatgg ataaaaaatt tgatttgctg gatcgctttc gagcagaagc
    29101 tgaaaaacag agctcgccta tcacccttag tatggggatt gcttatggct caagaaccct
    29161 cgatgaaatc ggtggagaag ctcagaataa tttagatgtt gctttagtcc gtggaggcga
    29221 ccaagttgtc gtgaaagagg taaaagaagg agcaaaacct gtttattatg gggggaaatc
    29281 tgctaagaat gtcaaacgca cgcgtgttcg ttctcgagcg atgagtaccg ctttgcgtcg
    29341 gatttttgat gaaacaggcg atgtgtttat catggggcac aagtttccgg atatggatgc
    29401 tttaggctct gcttttggtg tcagttgtct ggcacgcttc aaccagaaaa aagcttacat
    29461 cgttatcaac gagaacgaga tcattccaga tgtagaacgg tgtcttgaag aaatccataa
    29521 gcatcctgaa ttagaaatgc aactaatctc tccagaacgt gccatagagc tgaaaaaaga
    29581 agaggacctc ctcgtgatgg tggattacca taaaccttct ctatcgatct ctcagggcct
    29641 atatgagcaa tttgataaga ttgtgatcat tgatcaccat cgtcgtgggg aagagtttcc
    29701 aagtaaacct ctgctgactt atatcgaatc ttcagcttct tcagcttcag agctagtttc
    29761 tgagctgatc caataccaaa gcagcaaaac aaatcgctta gatcgttttg aggccacatt
    29821 attgcttgca gggatggttg tagatacaaa tagtttcaat gtccgtacaa ctgcacgaac
    29881 ttttgacgtt gccagctatt taaggtcttg tggagcagac gcatccttag tacaatattt
    29941 attgagctcg gatttagcga catacttgga gatcagcaat ttgattgctg gaagcgacta
    30001 tgtgacttct gatattgttg tggcggcagg gacagacgaa aaagagtata atagtgttat
    30061 agcagcaaaa acagcagata cattgctttc gatgatcaat atcaatgcag ctttcgtcat
    30121 tacaaagcgg acagacggct tgattgggat cagtgccaga agtaccggca cgatcaacgt
    30181 ccaaatcatc atggaatcct taggtggcgg aggacacttc acaaacgcag ctactcaact
    30241 ggaaaatacg acagttgagg aaacaaaaca gaagctattt gctgcaatca aagaaaatat
    30301 gaatcaaata tatgataagg agtaatccaa atgaaagtaa tttttttaca agatgtaaaa
    30361 ggaaaaggta aaaaagggga catcaaagaa gttccagcag gttatgcaca aaattatcta
    30421 atcaaaaata acttggcaaa agaagcaacg aaaagcagta tcagtcagtt agcaggacaa
    30481 aagaaagcac aagaaaaaca cgaagctgaa atcttggaag aagctaaaaa gttaaaagaa
    30541 ttcttggaaa aagaagatac tgtagtcgaa ttgaaggcga aagcagggga agacagccgt
    30601 ttgtttggtt cgatcccttc aaaacaaatt gctgaagggt taaacaaaca atacaaaatc
    30661 aaattagata aacgaaaaat cgaattagaa aatccgatcc gttcactagg ctacacaaac
    30721 gtacctgtta agcttcatca tgaggtgacg gctacgatca aagttcatgt cgtacaagaa
    30781 taacaaatct tgatagcgat gttcagctgg taagtataac cagaaggaaa aaagacaaac
    30841 cgaaatttat ctgagaaaac agctgcagca atgaaagcaa aagctgcaaa tcgatttcag
    30901 attgatttcg gtttcttttt tgggaacagc aaaatagcat aaagaaaacg agtataataa
    30961 aagagaacta tttttttatt acactttcgg tacgaatgat gacttttctc tcattttctt
    31021 gtaaaatgaa gcaattgaag acaaggagcg atacgtatga atgaagtgtg gcaggatcga
    31081 atcccaccgc aaaatattga agctgaacaa gcggttttag gctctgtttt tcttgacgca
    31141 gaaacgatca ttgatgccat ggagtacata gagccaaaag acttttatcg tcgtggccat
    31201 caaattattt ttgagacaat gatcgagtta aatgatcgta atgaagcgat tgacgtggtt
    31261 actgtcaaag accggctgga acaagccaat ttgttagaag atgccggtgg tttaagttat
    31321 ctctcagact tggcattagc agttccgaca gcagcaaata tcgtctacta tgcgaaaatc
    31381 gttgaagaaa aatcattgct gcgtacattg atccagacag caacaggaat cgttacgaaa
    31441 ggattcgagc aaggagaaga agttcaaaca attttagatg atgccgaacg cagtatcttg
    31501 gaggtttcgg aaaaaagaaa tcgaagcggc tttttatcta ttgctgaagt actgaactcc
    31561 acgattgaaa atatcgatcg actagctcaa aacaatgaag aaatcacagg tctaccaact
    31621 ggctatcaag gtctggataa aatgacagca ggtttccagc ccgaagaact aatcattctt
    31681 gctgcacgtc cagcggtagg taaaacagcg tttgcattga atattgctca aaatgtagga
    31741 acaaaaacag atcgttctgt tgcgatattt agtttggaaa tgggggcaga atctcttgta
    31801 aatcggatgc tttgtgcaga aggttcgatt gaggccagcc atttacgtac aggtcagctt
    31861 tcggaagaag aatggtcgaa tctgatcgtt gcgatgggga gtctttctcg agcaaatatt
    31921 tttatcgatg atacaccagg gatcaaaatc tctgaaatcc gtgcgaaatg tcggaaacta
    31981 gcgcaagaaa aaggcaatct aggcttgata ttgatcgatt atttgcagtt gattgaaggt
    32041 aacggtaaag agaaccgtca acaagaagta tcagatatct ctcgccaact gaaaaaactg
    32101 gcgaaagagc tgaaagtacc tgtcattgct ctttcacagt tatctcgtgg agtagaacag
    32161 cgacaagaca aacgaccagt gctaagtgat attcgtgaat ccggatcaat tgaacaagat
    32221 gcggacatcg tagccttctt gtatcgagat gattattatg aaagagaagg cggagatgac
    32281 gatgatggaa acgagccaaa aaatgacaac gtcatcgaag tcatcatcga gaagaaccga
    32341 agcggtgcac gaggaacagt tgaactactg tttatcaaag aatacaataa gttttcgtcg
    32401 ctttcgccaa gagaagaatt ttaaagaaga ggttgtgagc cttgagcaat cccgagagct
    32461 ctcgtaagaa gatgcaagga gtaaccgcag gaacgactag agtacttaat ttaaacagat
    32521 ctccaaaggg attgtgacaa aagtcttgtc acagtccctt tttccgaata aacggtgttc
    32581 aatcagctga cccttggtat tagctcagca aacgtcaaaa tatgaggaac tattttgacg
    32641 tttgtttctc taatactcag gtcaaaacgc tgatcttacg acctcttttc cttaaacggt
    32701 gttctcctgc caactcctcg gtcacaagtc acaatatcat caaacatgag aagcagtttt
    32761 ttgaaattgc tccttgtgac tcagagttaa acggcaggct cacaacctct ttcttaaacg
    32821 gtgttcaaaa gcttgcatct gcggtaatac gcccaatcaa acgaaaaata tgagaagcta
    32881 tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac ctctgattca
    32941 cggtgttcca aaagtagctt cttcgaaaaa taagtcgaaa tttcacaaaa atttgagaag
    33001 caattttcgt acattccttc ttatttcttg aagctgacca cttctgttac aacctctttt
    33061 ttaataacca ttctaaaaag ataaagttcg ttttttaagc gtttttataa aataaaaata
    33121 aaaatcattc gattttattt gaaatatgta tctattattg atacaatggt tcaggattaa
    33181 ataatataga acgaggtgtt cacatgtcat ctgtagtagt agtaggtacg caatggggcg
    33241 atgaagggaa aggaaaaatc acagactttc tcagtgaaaa tgctgaagtg attgcacggt
    33301 accaaggtgg cgacaatgca gggcacacaa ttaagtttaa cggtgtgact tataaactgc
    33361 atttgattcc ttctgggatt ttttataaag acaagatcag tgtgatcgga aacggcgttg
    33421 tagtcaatcc taaatcattg gttaaagaat tagcttattt acatgaaaaa ggtgtagaaa
    33481 caagtaattt gcgtatttct gaccgcgcgc acgtaatctt gccttatcat atcaaattgg
    33541 accaattaca agaagatgct aagggcgaaa ataagattgg tacaacgatt aaaggaatcg
    33601 gacctgccta tatggataaa gcagctcgtg taggtatccg tattgcagac ttattagata
    33661 aagaaatttt tgaagaacgt cttcgtttga atctagatga caaaaaccgt caatttgtaa
    33721 aaatgtttga ttcagaacca cttgattttg acgatatttt tgaagaatat tatgaatacg
    33781 gacaacaaat caaacaatac gtcacagata cttccgttat cttgaatgat gctttggatg
    33841 caggcaaacg cgtgttgttt gaaggcgcac aaggtgtaat gctggatatc gaccagggaa
    33901 cttatccttt tgttacttct tctaacccag tagccggtgg ggtaacaatc ggaagcggtg
    33961 tagggccttc aaaaatcaat aaagttgtcg gcgtctgcaa agcctatacg tcacgtgtcg
    34021 gtgatggacc atttccaact gaattatttg atgaaacagg agaacaaatc cgcgaagtag
    34081 gacgagaata cggtacgaca actggtcgtc cgcgtcgtgt aggctggttt gattctgtag
    34141 ttatgcgcca ttctaaacga gtttcaggta ttaccaacct atctttaaac tcgatcgatg
    34201 tattaagtgg attggaaaca gtaaaaattt gtacggctta tgaattagat ggcgaactaa
    34261 tctaccacta tccagcaagc ttgaaagagt tgaaccgctg caaaccaatc tatgaagaat
    34321 tgccaggctg gtcagaagac attacaggat gcaaaacact tgcagaactg ccagaaaacg
    34381 cacgtaacta tgtacgtcgt atctcagagc ttgttggtgt acgtatctca acattttctg
    34441 tcggacctga tagaacacaa acaaacatct tagaaagcgt ctgggcgcaa atctaaggat
    34501 aaaaacgacg actgaatcag gacgttctta ccagagcgag tcgaagtgtt tatctatacg
    34561 ataataataa cttgaacact gtttattcag aataagggac tgtgacaaaa accgtgtcac
    34621 agtccctttt tccgaa
//
LOCUS       DARBEC010000021        32502 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.021, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000021 DARBEC010000000
VERSION     DARBEC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32502
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.021"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..1664
                     /locus_tag="SCL80_000960"
     CDS             27..1664
                     /locus_tag="SCL80_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106848.1"
                     /translation="MKKNKNQIIDIIFMLFLSFAYVIPLFMSKGIYHSVNQDTYFHLS
                     RIIGLDNVWSSPVNFNNFDHHGTMMNIFYPWLTLYPAFLFYKMMGNLVLSYNIYYFFI
                     TFLTMVVSYFSMKQIKNNRYISLLFSIIYTFSAYRAIDIFHRASLGEAVALTFLPLIL
                     MGCYEIYIRDYQKWYWLSIGMTLVVYTHLLSVAMVSVFIGGTLFLSFYFWDQKIARLL
                     SLLKATVLTFFLSAGFLIPFIQQSRAQELKVPLGKELSGMAPSDMLTHILNNNYNNYT
                     IGLFLFLGLIGAFIFIKKLTADDLFIFFLGVFVLFCSTNLFPWQLFNHTPVKSLQFVW
                     RLNGFSSLFIAYTMSIVIYYIFSTKNNSWKIMVFTFLALILHLSGVSNSIHANKDSLT
                     LIAPEDAVAIAENYNHTDYANKESIYHPDMINNDQYLLGNQEINPTTHFMSNKLTIEL
                     DNSNNKNTVLTTPIYRYKGQVASINGKLVQTKLSKFGTTELTIPPGINKVVITYQYTK
                     LAIASRYLSIVTLILFLLYRFTFSKQKQPRREVQHSH"
     gene            complement(1809..2549)
                     /gene="truA"
                     /locus_tag="SCL80_000961"
     CDS             complement(1809..2549)
                     /gene="truA"
                     /locus_tag="SCL80_000961"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEG1106849.1"
                     /translation="MRNIKLTIEYDGKRYSGWQRLGDDDKTIQGKIEKVLHQMTNEEI
                     EIIGSGRTDAGTHARGQVANFKTNTEIGLAEMIDFMNRYLPRDIVIKRIEEMPERFHS
                     RYNAVGKKYSYYVWNNVIPSAFERNHSFYFPQELDMDKLNAACEKLVGTHDFIGFSAL
                     KKSKKSTVRTIDELSIERKGEMLHFTFVGDGFLYKMVRIIMGTLLEIGTGAMPIETID
                     EIFESKVRQHAGETVPAQGLFLDEVYYR"
     gene            complement(2740..3375)
                     /locus_tag="SCL80_000962"
     CDS             complement(2740..3375)
                     /locus_tag="SCL80_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1106850.1"
                     /translation="MDFLRKFGFFFLLLGVLSDFLTPYVLGLYYPELDQMKSVISVFG
                     EVSSPVRKAFLIWSVVSGIFFILSLPALYAAFVSTSRVLATLVTAAIAFYGIGDCIFT
                     GLFSINTHESSWNVSTWVHNIGSGLGYSGFLLFPVLLFLIYRKNGQMDVSHLYLGLSV
                     ISLLFAAIYGLARIPSINSWPLLNKIGFCQRISFFFNYVPIARLSILHLKQ"
     gene            complement(3522..4763)
                     /gene="sstT"
                     /locus_tag="SCL80_000963"
     CDS             complement(3522..4763)
                     /gene="sstT"
                     /locus_tag="SCL80_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HEG1106851.1"
                     /translation="MLEKFLDMSLVQRIVVGILVGILFGIFFPTWTFISILGTLFVSC
                     LKAIAPILVFFLTMASIAKHKIGNKTFVKPILILYLVGTLLSALVAVIVSSIFTVPIT
                     LQETVSEKAPQSLESVLSTMLTNVTQNPVQSLIDANYLGVLFWAVLLGLAFRARSEST
                     KELVDQISIALSQVVQVIIAFAPIGILGLVYQSIATTGIAGLAEYLQLLLVLIGTMLF
                     VALVVYPFLTFLFIKENPYPLIFFCLKESALPAFFTRSSAANIPINMMLAERLKLTKE
                     SYSISIPLGATINMGGAAITISLMTLTAVHSLGIEAPFVLKVLLCILSALAACGASGV
                     AGGSLLLIPLACSLFGISNDVAMQIVGIGFIIGVIQDSMETALNSSSDLLFTAIGELG
                     ARKRSGEKIKLKDCLDGLSER"
     gene            4961..5959
                     /locus_tag="SCL80_000964"
     CDS             4961..5959
                     /locus_tag="SCL80_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106852.1"
                     /translation="MFLSNFFVRCLFLKKIKKESYGFLTIFVACIMMVLQRILISAVT
                     IPTYATNPGRFYIYTFLQTSVVIGANLIPLYMGYQYQKIARLKVLHYLSRFAFIFLIA
                     TLVCNIFFYVQAGSLNIRDYWLILTPISQNYFTYAVSCTLLFCSIPYTVGFLNKLSKT
                     TIKYLLLFLTIGLVGCSTLFNKDPFALQNGQSILWIFYLFLMGYGLKEWNWKEKVRYK
                     LPQVFISLIVLFGLIILMTQISLIIRGNTDTALRFSKPYSLFAMYYTFSSFLFLEMLS
                     DKLGIKGRGNFLAVFLIVTQVICNFPLVSYRVSTFMKREFPNSGKAWLINIGQFFI"
     gene            5972..7990
                     /locus_tag="SCL80_000965"
     CDS             5972..7990
                     /locus_tag="SCL80_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HEG1106853.1"
                     /translation="MSLLVIFLFYIQKIRFIHRFIEYFAFDSIEQLTQRWKKRIHWLF
                     VHKSYFLVAAFFYCFTFVQIFVLEPKEGWKETIQVALKIFTQRQAPMILTIIIIMGFF
                     FLLLLISNRFWYALTATLIINLLLTISTVIKMEMREEPVFPSDLKMLTGLSELLSMVS
                     PVLLIVGGLILLLLLITSIIVQRRLQKQYSLKIHWKRRFISIAVLLGCFSGVFFINHK
                     NSPSFLLFNLFKVNKTFFNQQGAVKDNGPVIQFLNNIDIKIMEEPAGYSKEKINGIMK
                     KYNTKADEINQTRSDWLNNQTIVLNLSESFSDPARVPNLTVPTDPIPYIKSIITEGTG
                     GLMLSVGYGGGTANMEWEGLTGLSISNLSASLVTPYTQLVERQKISPNITDLFDEKIA
                     IHPFTAALYRRKQVFDKFGFDRFYYLDSPDKLTYTDKIEQSPRISDDSAYKETLKALQ
                     SNTETSQFIQLSTMQNHMPYDDYYSELNYTAEGTAVIPNRRKELQTYMQGLHYTDTAV
                     KKFIQEIDKIDKPITFVFYGDHLPAIYSGNDSKKYGLEQHQTDYFIYNNKYSREQAAT
                     LNKEVVAPNNFGAMALEHANIKVTPYYALLTEVSNHLPALTIDPTESLSNRFNGKQVF
                     VTEDSQIIQEKNLTEKQKEILEDYRLIQYDLVAGNQYSATWATQKIKD"
     gene            complement(8050..8766)
                     /locus_tag="SCL80_000966"
     CDS             complement(8050..8766)
                     /locus_tag="SCL80_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEG1106854.1"
                     /translation="MKKRILVTIGLAFLLAGCTNGNESKKESTKETAKATVATTTTTS
                     TESKSTAASTETADTSTSSESSKTTETTQTSTSSSSVEASSSSSESSEQVEQTLWNAD
                     KSAQLEAFMTKWGQTMGQQYKSYTNQMSVDLYGLKVPQAILNGEWKMAIGGVPVSAEW
                     SESGTGQADYQITAVYSDAETEPYLKKHVYLFGFQQNQPKVLVTQQNQGNPDNYLYFN
                     ETANNELKNGFNQIVYGQAN"
     gene            complement(8993..9535)
                     /locus_tag="SCL80_000967"
     CDS             complement(8993..9535)
                     /locus_tag="SCL80_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1106855.1"
                     /translation="MNIVEEKFVQETKQADIDHHKPTAGAMVGHVLANFKLEEMKLIQ
                     AQLYVLGTSSQELSTLFSEYADQEARWFNKISHELVIENEIVPSTLEEVMRYGKIYEH
                     GQNKYLPASTMLENFAKDFDFQNVFITRAIKLAEKEEKYGLQQLLVELLSFNKEIIYQ
                     VQRLQGKTVRQDLDEEDDDD"
     gene            complement(9540..9767)
                     /locus_tag="SCL80_000968"
     CDS             complement(9540..9767)
                     /locus_tag="SCL80_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1106856.1"
                     /translation="MKKIIMQLDTLACPSCMQKIEHAVSQQPGVSNTKVLFNASKIKA
                     NFDPDEVHPEQLAAVVEKLGYEVQSAKVKEA"
     gene            complement(9846..11726)
                     /locus_tag="SCL80_000969"
     CDS             complement(9846..11726)
                     /locus_tag="SCL80_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1106857.1"
                     /translation="MKLQKWIQEHRNHITALSAVLIIFGFIGKFTGFVLFYNIALAIA
                     SIIAVIPIMIHAYQAVKVKVISIDLLVTIAVLGAFMIGEYKESAIVTFLFLFGSYLEQ
                     KTLTKTRESIKALTEMAPSTASVVQEDGTTEEVEIDDVEEGDIVLVKTGGAIPVDGRV
                     IDGKGYADEAAVTGESRQVVKISGDEVFSGTILADGYVTIEATKVGDDTTFAKIIELV
                     EEAQDTKSHAEKFIDRFSQYYTPTVLLIALVVGLISRDFRLAITVLVLGCPGALVIGA
                     PVSNVAGIGNGAKRGILVKGGEAMDTFSKIDTFVFDKTGTLTKGTTAVSTIKNYTEDE
                     GDSLALVARLETLSDHPLGRAVIHYAQEKDIDFQQLAVKDNQTIKGQGITAEIDGHTV
                     CAGNTKLIAAHKLSLTPKQVQDLHQLQQTGSSVIIVAIDKKITQIIGVSDVIRPEVAE
                     QLAKLKQAGAKQLVMLTGDNQMTADYVADMLGIDEVHAELLPDEKVQFVKKYQEQGLR
                     VAFIGDGINDSPSLAAADIGIAMGSGTDVAIETSDVVLMQSNFESLVHAYRLAKKTVL
                     NTRENVVIAIGVVLFLLNGLFAGFIYMASGMFVHEASILIVIFNAMRLLYFGHESKEE
                     KS"
     gene            complement(11915..12529)
                     /locus_tag="SCL80_000970"
     CDS             complement(11915..12529)
                     /locus_tag="SCL80_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106858.1"
                     /translation="MKLTFKPLEKKDYKKAIYFAVEGMNFERYMENELLLNLYGRYFL
                     YMELNRATQILALYAEDELAGVLLAEIKGEKKKHRSFGQQIYVKIFDFLQNIFAKESK
                     GAYETANEQMLAAYLKKHSPDGEIIFLAANPDLKTKGIGTKLLTEFEKREKGKEIFLF
                     TDSGCTYQFYERRGFERLKEKISKIKILDKEIDLRCMLYRKIIQ"
     gene            12671..13231
                     /locus_tag="SCL80_000971"
     CDS             12671..13231
                     /locus_tag="SCL80_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1106859.1"
                     /translation="MKKTDRRVKKTEKALAETLSTLLVNKKIQAITIRELTETADVHR
                     STFYTHYKDIYDLYDQLESNFFRDLNEILSYDPAHSYEELYTRLIDYVYANTFLYRLF
                     ILNHGDISFQKKVKQVIEENYLNIWLYEDKKTIITEEMRFFTTYHVQGCLSVLNRWVE
                     SNYKYSKKELLELLQKLNDHIEKIMP"
     gene            complement(13536..13850)
                     /locus_tag="SCL80_000972"
                     /pseudo
     CDS             complement(13536..13850)
                     /locus_tag="SCL80_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
     gene            complement(14104..15739)
                     /locus_tag="SCL80_000973"
                     /pseudo
     CDS             complement(14104..15739)
                     /locus_tag="SCL80_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294751.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(16294..16857)
                     /locus_tag="SCL80_000974"
     CDS             complement(16294..16857)
                     /locus_tag="SCL80_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1106860.1"
                     /translation="MITVVHKERQEKSSSDVRLWVYLGSISTKIQYGYTDSAKKQGNV
                     KFLRITDIQEGRVNWSSVPYCDISNSKLVDLRLEENDILIARTGGTMGKSFLVKEISE
                     ESVFASYLIRIRLVEKLLSEYVDCFLDSPLYWKLLEKISYGTGQPNVNGTNLSKLLIP
                     LPPLEEQQRMTTKIEMIRRSIRRINFE"
     gene            complement(16872..17915)
                     /locus_tag="SCL80_000975"
     CDS             complement(16872..17915)
                     /locus_tag="SCL80_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106861.1"
                     /translation="MTQRVAIMILVLLVIGLLVYYVLKFKHWKQQRIHQDIEKKLKRY
                     PIVQAAWEKAEAKQYNIPGLTETRMVVPETGENEVCQWMTPQGLAFSQDFVFISAYCY
                     DHQHHSIIHVLDRETGQPIKLLILPKRPHVGGLVYDTKRELLWLTITGSATGRVAALR
                     LIDILADTSEETGQPIAYWLTTDLSEIPQASYLTQNNDQLVSGNFTLKGEGQLTFYLL
                     PTIAEMKTAIRRKDKIQPTVTMSRKTSDKIQGVAFYGHYLLASRSYGPYTSELLVSER
                     DSPSRILKRIKFPPYLEQIVVVEDRLAVLFESGAAAYREKANPVLANVLLLDLATLLH
                     ANKRPKKIAATKK"
     gene            complement(18048..18452)
                     /locus_tag="SCL80_000976"
     CDS             complement(18048..18452)
                     /locus_tag="SCL80_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1106862.1"
                     /translation="MKKMYETAMINRGGRDGEVEAPNGSMHMKIDRPGIHSEGTNPEQ
                     LFAAGYASCFNGAVQHMLEENNLESDSEVKARVSLFQLEDGGYQIGVVLEVSLPGLEK
                     DQAEKIAEEAHAFCPYSKATRGNIDVEVTIVE"
     gene            complement(18685..19320)
                     /locus_tag="SCL80_000977"
     CDS             complement(18685..19320)
                     /locus_tag="SCL80_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HEG1106863.1"
                     /translation="MIFLQKYGFYFLLLGVMSDLSTPYILGLFYPKLNQMTTVISVFG
                     DVDSPVRRAFLVWSVVSGLFFVLSLPALYHLFVGTSKTLAILVVATVGLYGIGDCIFT
                     GLFSINTNESSCNLSTWIHNIGSGLGYAGFLLFPLLLVLLYRQSGQGTLSHFYLVLTV
                     ISLLIAGLYGLARIPSISQFAFFKQLGFFQRLSFFFNYLPMICLSFFQLRK"
     gene            complement(19586..20638)
                     /locus_tag="SCL80_000978"
     CDS             complement(19586..20638)
                     /locus_tag="SCL80_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106864.1"
                     /translation="METSQKISPKIFLNKVLAGTATGIIIGLIPNAVLGAILKYFSAY
                     PFAVTIAQLAVIFQLATPLIIGGLIALQFGFDPMRMMVVAGASFVGSGVVKFTPDLGE
                     TGVYVGAGTGDIINTMLTASIAVGLILLIGNSFGSVSIVLTPIVVGVGAGLLGFYLFP
                     FVTAITSAIGSLINNFTTLQPLLMSILIGCSFAFLIISPISTVAIGMAIQLDGVSAGA
                     AAMGVAATTFVLVVNSWKVNKSGVTIAIALGAMKMMMPNLFRKPVILIPCLVSAVITA
                     IPVALFSISGTPASAGFGVVGLVGPLASLDAGLNGFLVVLCWLVIPVFAALITQFLCE
                     KVFKLYDREEVFKFLG"
     gene            complement(20651..21589)
                     /locus_tag="SCL80_000979"
     CDS             complement(20651..21589)
                     /locus_tag="SCL80_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015081805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1106865.1"
                     /translation="MKIAIAGAGAMGSRFGLMLHQSGNEVLLIDGWAEHVQQIKEHGL
                     QANFNGKEVEAKLPIVLQSEVEKEDQVDLIILFTKAMQLEKMLQDIQSLIKKDTEVLC
                     LLNGIGHEDIIEKFVPMENIYIGNTMWTAGLEGPGQVKLFGSGSVELQNLGDGKEAAA
                     KKLADKLSESGLNAHFSDNIHYSIYRKACVNGTMNGLCTILDVNMAELGKTSTAHKMV
                     ATIVNEFAKVAAVEKIELDVPEVIAHCESCFDPETIGLHYPSMYQDLIKNHRLTEIDY
                     INGAISRKGKKYGVATPYCDFLTELVHAKEDSLNVK"
     gene            complement(22040..22690)
                     /locus_tag="SCL80_000980"
     CDS             complement(22040..22690)
                     /locus_tag="SCL80_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106866.1"
                     /translation="MNDEYKNDEDKMLFEEIENRCRLNFELRGKMSLIQQKKYLANKS
                     EFTLGHVEKLISDWISSRSEFTKIKQPIKFDMKKLLLNKSEIGNRDQYIRAKGQEIID
                     SLGEMRSYNYLYVTHRADGMVITVGKSSSNDIFLDGDLFYQLNINHLSGTENIILRTE
                     YGNEIFAKYDEILKNYLDWAWIIPVESGDAKKLERLLGDELINKKVPILNYYSHRQ"
     gene            complement(22790..23299)
                     /locus_tag="SCL80_000981"
     CDS             complement(22790..23299)
                     /locus_tag="SCL80_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1456 family protein"
                     /protein_id="HEG1106867.1"
                     /translation="MNNNDRLLRLRYAIDLKDTDLIKAFELGGVPLTKEEAQAVLTKV
                     QDKNKDNAENNVYEKTINNQVFDAFLNGLILLARGPQKDKPIVDTPQKSKEIKYINNV
                     LLKKLKIALSMTTDDILDVFAEAEIYPSKGEIGAFLRKEGQRNFKPCGDKYMRNFLKG
                     LGIYNRRKV"
     gene            complement(23500..24369)
                     /locus_tag="SCL80_000982"
     CDS             complement(23500..24369)
                     /locus_tag="SCL80_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1106868.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            complement(24389..25510)
                     /locus_tag="SCL80_000983"
     CDS             complement(24389..25510)
                     /locus_tag="SCL80_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1106869.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            complement(25512..25907)
                     /locus_tag="SCL80_000984"
     CDS             complement(25512..25907)
                     /locus_tag="SCL80_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1106870.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            complement(25926..26732)
                     /locus_tag="SCL80_000985"
     CDS             complement(25926..26732)
                     /locus_tag="SCL80_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1106871.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            complement(26725..27513)
                     /locus_tag="SCL80_000986"
     CDS             complement(26725..27513)
                     /locus_tag="SCL80_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106872.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            complement(27527..28003)
                     /locus_tag="SCL80_000987"
     CDS             complement(27527..28003)
                     /locus_tag="SCL80_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1106873.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            complement(28075..30732)
                     /locus_tag="SCL80_000988"
     CDS             complement(28075..30732)
                     /locus_tag="SCL80_000988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1106874.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            complement(30751..31155)
                     /locus_tag="SCL80_000989"
     CDS             complement(30751..31155)
                     /locus_tag="SCL80_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1106875.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            complement(31387..31644)
                     /locus_tag="SCL80_000990"
     CDS             complement(31387..31644)
                     /locus_tag="SCL80_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEG1106876.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            complement(31769..32101)
                     /locus_tag="SCL80_000991"
     CDS             complement(31769..32101)
                     /locus_tag="SCL80_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106877.1"
                     /translation="MPLDLSTGRKIRFSEIQYEGIGFTLIDLKNSTFEIKEGLDLKNL
                     LCADDKIKKIQLYKVPILTPTVLTMLEMENNINYIDSGSDLLIRWQVITNFLMELKKY
                     SNTQTKFS"
BASE COUNT        10239 a         6444 c         4914 g        10905 t
ORIGIN      
        1 ttattacttt taaataggag aaaaaaatga aaaaaaacaa aaaccaaata atagatatca
       61 tttttatgtt atttctaagt tttgcttacg tcatccctct attcatgtct aagggaatct
      121 atcatagtgt caatcaggat acttactttc atttatctcg aatcatagga ctagataatg
      181 tatggagttc tccagtaaac ttcaataact tcgatcatca tggaacaatg atgaatattt
      241 tttatccatg gcttactctt tatccagctt ttttatttta taaaatgatg ggaaatcttg
      301 ttttaagtta taatatctac tattttttta tcactttttt aacaatggtc gtttcttatt
      361 tttctatgaa acaaataaaa aacaatcgtt atatttcgct gttattttcg attatttata
      421 cattttccgc ctatcgtgcc attgacatat tccatcgtgc ttcattggga gaagctgtcg
      481 ctttaacatt tttgcctttg atactaatgg ggtgttacga aatttatatt agagattacc
      541 aaaaatggta ctggttgtct attggcatga cgctagtagt gtatactcat ttactttctg
      601 tcgctatggt ttctgtattt attggaggaa cgttgtttct ttctttttat ttttgggacc
      661 aaaaaatcgc tagactctta agtcttttaa aagctaccgt cctcaccttt tttttaagtg
      721 cgggattctt aattcctttt attcaacaat ctagagcaca agaattgaaa gtcccactgg
      781 gaaaagaatt aagtggtatg gctcctagtg atatgctgac acatatttta aataataact
      841 ataacaacta tactattgga ttattcttat ttctcggatt gattggtgct tttattttta
      901 taaaaaaatt aactgctgat gatttattta ttttctttct tggtgtattt gttctttttt
      961 gttcaaccaa tttatttcct tggcaacttt ttaaccatac accagttaag agtctacaat
     1021 ttgtctggcg gttaaatggt ttttcttctt tatttatagc ctatacgatg agtatagtga
     1081 tctattatat tttttctact aaaaataatt catggaaaat aatggtcttt acgtttcttg
     1141 cactaatatt gcatctctca ggagtaagca attctatcca tgctaacaaa gattcattaa
     1201 cacttatagc acctgaagac gctgttgcta tagctgaaaa ttacaatcat actgattatg
     1261 caaacaaaga gtcaatctat catcctgata tgattaataa tgatcaatat ttactaggta
     1321 accaggaaat aaatccaaca actcatttta tgagtaacaa acttacaatt gaattagata
     1381 attcgaataa taaaaatact gtcttgacga cacctattta tcgttataaa gggcaagtgg
     1441 cttctattaa tggcaaacta gtacaaacaa aattatctaa gtttggcaca actgaattaa
     1501 ctattcctcc aggtataaat aaagtcgtta ttacttatca gtacacaaaa ctagcaattg
     1561 cttcaagata tttatctatc gttactttga ttttattttt actttatcga tttacttttt
     1621 ctaaacaaaa acaaccgaga agagaagtac aacattctca ctagattttt atttaagaaa
     1681 gactttttag ctgtacacat aaacaggcta aaaagtcttt ttttatattt ctttaatttg
     1741 cttcatacat tatttaacat cgatatttat actgtgaaac atgcgtttca tccattatca
     1801 aagtattttt atcgataata aacttcatcc aaaaacagac cttgtgcagg gacggtttct
     1861 cctgcatgtt gccgcacttt gctttcaaat atttcatcga ttgtttctat cggcatggca
     1921 cctgtgccga tttctagtaa tgtccccatg ataatccgca ccattttata taagaatccg
     1981 tctccgacaa aagtaaaatg aagcatttct ccctttcgtt cgatagataa ttcatcaatc
     2041 gtacgcactg tggatttttt tgattttttc aatgcagaaa aaccaatgaa atcatgtgta
     2101 cccactaatt tttcgcaagc tgcattcagc ttgtccatat ccaactcttg agggaaataa
     2161 aagctgtggt ttcgctcaaa tgctgaagga atcacgttgt tccaaacata atagctgtat
     2221 tttttgccca ccgcattata tcttgaatgg aagcgctcag gcatttcttc gattcttttg
     2281 atgacaatat cccgcggaag gtaacgattc ataaagtcga tcatttctgc cagtccaatt
     2341 tctgtgtttg ttttaaaatt tgctacttgt cctcgcgcat gggtgccagc atctgttctt
     2401 cctgatccaa taatttctat ctcttcattt gtcatctgat gcaatacttt ttcgattttt
     2461 ccttgaatgg ttttatcgtc atctccaagt ctttgccatc ctgaatatct ttttccgtca
     2521 tattcgattg tcaacttgat gtttctcata ttttagtggc tcctctgtct tccatttggt
     2581 cttctctcta cctttttaag attgtagcac aaaatatagc tccttgcttc tctaagacag
     2641 aagctataca agaaaactgt ggaaatatat atttcagaaa aacaaaaaac agccaccgga
     2701 tgaagtatcc gctgactgtt caatttgacg gctcaagttt tactgtttga gatgcaagat
     2761 actcaacctg gcaatgggca cataattaaa gaaaaaactg attctttggc aaaaacctat
     2821 tttattcagt aatggccaag aatttataga aggaattctt gccagtccat aaatcgccgc
     2881 aaaaagcaaa ctgatcacag acagtccaag gtataagtgg cttacgtcca tttgcccatt
     2941 ttttcgatag atcaagaaaa gaagaactgg aaacaataaa aaaccactgt aacctaaacc
     3001 tgatccgata ttatggaccc atgtcgagac attccacgaa gattcatggg tattgatact
     3061 gaacaaacct gtaaagatac aatcacctat accatagaac gcgatggctg ctgtaactag
     3121 ggtagctaac acacgagatg tactaacaaa tgcagcatac aatgcaggca atgacaggat
     3181 gaaaaagatc cctgaaacca cagaccaaat aagaaacgct tttcttacag gactggaaac
     3241 ctctccaaat acactgatca cgctcttcat ttgatctaat tcaggataat acaatcctaa
     3301 tacgtacgga gtcaaaaaat cactcagcac gcctaataat aaaaagaaaa aaccaaattt
     3361 acgtaaaaaa tccatgatac gcctccaata acaattacct atgcttttaa ttgttattat
     3421 aaacgacttt atcttgattt aacagaaacc tctatttaca cgaacatcta tataactcat
     3481 taaaaactcg aatcatttta aaaatactaa atatctaacg ttcaacgttc acttaatcca
     3541 tctaaacaat ctttcaattt gattttttcc ccacttcttt tacgcgcgcc tagctctcct
     3601 attgcagtaa acaacagatc acttgacgaa tttaaggcag tttccattga atcctggata
     3661 acaccgataa taaatccaat accgacgatt tgcatcgcga catcattaga gataccaaac
     3721 aaactacatg ctaatggaat caataacagt gaaccgcccg caacacctga agctccacaa
     3781 gcagctaaag cagacaaaat acataaaagg actttcaaaa caaaaggtgc ttcaatccct
     3841 aaagaatgta cagcagtcaa cgtcatcaag gaaatggtaa tcgcggcacc gcccatattg
     3901 atcgtagcac ctaacgggat cgatatggaa taagactctt ttgtcaattt caatctttca
     3961 gccaacatca tgtttatcgg gatatttgcg gcagaacttc gggtaaagaa agcgggaagt
     4021 gcgctttctt tcaaacaaaa gaaaatcaat ggatagggat tttctttaat aaacaaaaac
     4081 gtgaggaatg ggtacacaac taatgcgaca aacagcatcg taccgatcaa aacaagtaaa
     4141 agctgcagat actctgccaa tccagcgatt ccagtcgtag caattgattg ataaaccaat
     4201 cctaaaatcc ctatcggagc aaatgcaata atcacttgta cgacttgtga caacgctata
     4261 gatatctgat ccaccaactc ttttgtcgat tctgaacgtg ctcgaaaagc tagtcccaac
     4321 aatacagccc agaataagac gccaaggtaa ttcgcatcaa taagagattg aacaggattt
     4381 tgtgtgacat tcgtcaacat agtagataaa acactttcca aactttgcgg tgctttttca
     4441 gatactgttt cttgtaaagt aataggaaca gtaaaaattg aacttacaat aactgctaca
     4501 agtgcagaaa gcaatgttcc cactaaatat aagatcaaaa taggcttcac gaatgtctta
     4561 ttcccaattt tatgcttggc aattgacgcc atcgtcaaga aaaaaaccag aataggtgcg
     4621 attgccttca agcaactgac aaacaacgtt cccaatattg aaataaatgt ccatgtgggg
     4681 aaaaagatac caaacaatat tccgactaaa ataccaacaa caatacgttg aactaacgac
     4741 atatccaaaa acttctctaa caaatgacca ccctttcaca attttaacaa tcattgtatc
     4801 attttatgca aagaaactaa aggtataatt ttctaaaaat tctaacaata caaacaacca
     4861 tttcttgcta attttgtact ttttctgtct acacgttgtt ttttgagata ttctaagtta
     4921 ggatgttatt aatgggcaac ttatcaggaa acgttttctt atgtttttgt ccaatttttt
     4981 tgtgaggtgt ttattcttga aaaaaataaa gaaggagagt tatggcttcc tgacaatctt
     5041 tgtcgcttgt ataatgatgg ttttacagcg tatattgatt tcagcagtca ctattcctac
     5101 atatgcaaca aaccctggtc gattctacat ctatacattt ttacaaactt ccgttgttat
     5161 tggtgcaaat ttgatcccac tttacatggg ttaccaatat caaaaaatcg cccgtttaaa
     5221 agttttacat tatttaagtc gttttgcctt tatattccta atagccacac tagtttgcaa
     5281 tatctttttt tacgtacaag ctggcagctt gaatattcgt gattattggc ttatcctaac
     5341 acctatcagt cagaattact ttacttatgc tgtttcttgt actttgcttt tttgtagtat
     5401 tccttataca gttggatttt taaataaatt atcgaaaaca acaattaaat atctactatt
     5461 atttttaaca attggattag ttggctgctc tactctattt aataaagacc cgtttgcatt
     5521 acagaatgga cagagtattt tatggatttt ttatttattt ttaatgggtt atggtttgaa
     5581 agaatggaac tggaaagaaa aggtccgcta taagcttcct caagtattta tcagcttgat
     5641 tgttttattc ggtttgatta ttttgatgac acaaatttca ctcatcatca gaggaaatac
     5701 agatacagcg ctgaggtttt ctaaaccata ttctctcttc gcaatgtatt atactttctc
     5761 ttcgttttta tttttagaaa tgctatctga taaattaggt ataaaaggaa gaggaaattt
     5821 tcttgctgta tttttgattg tcactcaagt tatatgtaat ttccctttag tttcttatcg
     5881 cgtatctaca tttatgaaac gtgaatttcc aaactcaggg aaggcatggc tgataaatat
     5941 cgggcagttt tttatttgat atttattagc agtgtccctc ctagttatct ttttatttta
     6001 tatacaaaaa atccgtttca ttcatcgttt catcgaatac ttcgcttttg attcgattga
     6061 acaattaact cagcgttgga aaaaaagaat tcattggctt tttgttcata aaagttattt
     6121 tttagttgca gcattttttt actgctttac cttcgtgcag attttcgttt tagaaccaaa
     6181 agaaggttgg aaagaaacta tccaggttgc cttaaaaata tttactcagc gacaagcacc
     6241 catgatcctg acaatcatca ttattatggg atttttcttc ttactattgt tgatttccaa
     6301 tcgtttttgg tacgccttga ctgctacctt gatcattaac ttactgctca ctatttcgac
     6361 agtaatcaaa atggaaatgc gggaagagcc tgtctttcct tcagatttaa aaatgctgac
     6421 cggattgtca gaactattat cgatggttag cccagtctta ttaatcgtgg gcggattgat
     6481 ccttctccta ttattgatta ctagtattat tgtacaacgt agactacaaa aacagtattc
     6541 cttgaagatt cattggaaac gtcgatttat cagtatcgct gtattattag gatgtttttc
     6601 gggtgtcttt ttcattaatc acaaaaattc gccttctttc ttgctgttta atttatttaa
     6661 agtaaacaag actttcttta accagcaagg cgccgtaaaa gataacggac cagtcattca
     6721 attcctaaac aatatcgaca ttaaaatcat ggaagaacct gctggctatt cgaaagaaaa
     6781 aattaatgga ataatgaaaa agtacaacac aaaggcagat gaaatcaacc aaacaagaag
     6841 tgactggtta aacaatcaga cgatcgttct caatttaagt gaaagcttca gtgatcctgc
     6901 acgtgtccct aatctgacag tacctacaga ccctattcct tacatcaaaa gcattataac
     6961 agaaggaaca ggaggattga tgttatccgt cggttatggt ggcggaacag ctaacatgga
     7021 atgggaagga ctgacaggct tgagtatcag taacctgtct gcttcgttag ttacacctta
     7081 tacccagcta gtggagcgtc agaaaataag tccaaatatt accgatctat tcgatgaaaa
     7141 aatagccatc catcctttta cagctgctct ttatcgcaga aaacaagtgt ttgataaatt
     7201 tggttttgac aggttctatt atttagacag tccggataaa ttaacttata cggataaaat
     7261 agaacaaagc ccacgcatat cggatgattc agcatacaaa gaaaccttga aagctttaca
     7321 aagcaatacg gaaacgtcac aattcatcca attatccact atgcaaaacc atatgcctta
     7381 tgatgattac tatagtgaat tgaactacac tgctgaagga actgctgtca ttcctaatag
     7441 aagaaaagaa ttacaaactt atatgcaagg ccttcactat accgatacag cagtaaaaaa
     7501 attcatccaa gaaatcgaca aaatcgataa accgattacc tttgtttttt atggtgacca
     7561 tttacccgca atctatagtg ggaatgattc gaaaaaatat ggattagaac agcaccagac
     7621 agattatttc atttataaca acaagtatag tcgtgaacaa gcagctacgt taaataaaga
     7681 agttgttgct cctaataact ttggtgctat ggctcttgag catgcgaata tcaaagtaac
     7741 tccttactat gcgttgttga cagaagtatc taatcatcta cctgcattaa caattgatcc
     7801 tacagaaagc ttgtctaatc gttttaatgg aaaacaggtc tttgttacag aggatagtca
     7861 aattatccaa gaaaaaaatt taactgagaa acaaaaagaa attctagaag attatcgtct
     7921 gatacaatat gatcttgttg caggtaacca gtacagtgca acatgggcga cacaaaaaat
     7981 aaaagactaa caaataaact gagcaagggg ttgtgataaa agacatgtca caaccccttg
     8041 tttatctcat taatttgctt gtccataaac gatttgatta aagccatttt tcaattcatt
     8101 attcgctgtc tcgttgaagt aaagataatt atcgggattc ccctgatttt gttgtgtcac
     8161 caatactttt ggctgatttt gctgaaaccc gaatagatag acgtgctttt tcagatatgg
     8221 ttctgtctct gcatcggaat agacggctgt gatttgatag tctgcttgac ctgtcccact
     8281 ttctgaccat tctgcagaaa caggcactcc accaatagcc atcttccact ccccgttcaa
     8341 aatagcttga gggactttaa gaccatataa gtccacactc atttgattcg tataactttt
     8401 gtattgttgt cccattgttt ggccccattt ggtcataaat gcctctaatt gtgcggattt
     8461 gtctgcattc cacagtgttt gttcaacttg ttcgctgctt tctgaagagg aagatgaagc
     8521 ctctacagaa gaacttgaag tagatgtttg agttgtttca gtcgttttgg aagactctga
     8581 cgaagtagag gtatctgctg tttctgtact tgctgcagtt gacttgctct ctgtgctagt
     8641 tgttgtagta gttgcaactg ttgctttcgc tgtttctttt gttgattctt ttttcgattc
     8701 attcccgttg gtacaaccag ctaacaaaaa agctagacca attgttacta aaatcctttt
     8761 tttcaattaa aaaactcctt tcgattactc ccacttcttt attctatcaa ataagcaatg
     8821 tgattttatt gatttttcgt attttctcat caatctttca aatcttctaa aaactgagaa
     8881 gcacccgctg atgttgtctg taatacatca gcgggtgctt cttttatttt aatcttcgtg
     8941 attttccaat ttacctcacg ctttatattt gtatttgaag aaacatagac ttttagtcat
     9001 catcgtcttc ctcatccaaa tcttgtcgga cagtttttcc ttggagtctt tgtacttggt
     9061 agatgatttc tttattgaaa cttaaaagtt caacaagcaa ttgttgcaag ccatattttt
     9121 cttctttttc agctaatttg attgcccgtg tgataaagac attttggaaa tcaaaatctt
     9181 ttgcaaagtt ttccaacatt gttgaggctg gtaaatattt gttttgacca tgttcgtaaa
     9241 tttttccata acgcatgact tcttctaaag tagacggaac gatttcattt tcaatcacta
     9301 actcatgaga gattttattg aaccatctcg cttcttgatc agcatattct gaaaaaaggg
     9361 tacttaattc ttgcgaagat gtacccaaaa catacaattg tgcttggatc aatttcattt
     9421 cttctaattt aaaatttgct aatacatgcc ccaccatggc gccagcagtt ggtttatgat
     9481 ggtcgatatc tgcttgtttt gtttcttgaa caaatttttc ttccactata ttcattgatt
     9541 tacgcctctt ttacttttgc tgattggact tcataaccta atttttccac aactgcagct
     9601 aattgttcag ggtggacttc atctggatca aagtttgctt tgattttgct cgcattaaac
     9661 aaaactttcg tattgcttac cccaggctgt tgagaaaccg catgttcgat tttttgcata
     9721 cagcttggac aagctagtgt atctaattgc ataataattt ttttcatcgt agttcttcct
     9781 tcctttttct tgatagtctt attctagatt acagcttcca taaaaaaatt gattcttatc
     9841 aagttttacg atttttcttc tttgctttcg tgtccaaaat acaataaacg catcgcgtta
     9901 aagataacga tcaagatact agcttcgtga acgaacatcc cactagccat gtaaataaac
     9961 ccggcaaata agccgttgag taaaaataag acgacaccaa tggcaatcac tacattttca
    10021 cgggtattta aaacagtttt cttagccaag cggtaggcat gaaccaacga ttcaaaatta
    10081 gattgcatca agacaacgtc actcgtttcg atagcaacat ccgttcctga tcccatagca
    10141 atcccgatat cagccgcagc caaagaggga gaatcattga ttccatctcc aataaaagca
    10201 acgcgcaatc cttgttcttg gtattttttc acaaattgaa ccttttcgtc tggtaataat
    10261 tcagcatgga cttcatcaat gcctaacata tcagctacat aatcagcagt catctgatta
    10321 tcacctgtca gcatcactaa ctgttttgct cctgcttgtt tcaacttagc taattgttca
    10381 gcgacttctg gtcgaatcac atctgacaca cctatgattt gagtgatttt tttgtcgatt
    10441 gctacaataa tgaccgagct gcctgtttgt tgtaattgat gtaaatcttg tacttgcttt
    10501 ggtgtgagag aaagtttgtg agcggcaatc aatttggtgt tgcccgcaca aactgtatga
    10561 ccatcaatct cagctgtgat tccttgtccc ttgattgtct gattatcttt gactgctaat
    10621 tgttgaaaat caatgtcttt ctcctgagca taatggatca cggcgcgccc cagaggatga
    10681 tcggataacg tttctaatcg agccactaaa gctaagctgt ccccttcatc ctctgtatag
    10741 ttcttgatag tggatacagc agtagttcct ttggtcaatg taccagtttt atcaaagaca
    10801 aaagtatcga ttttggaaaa agtatccatt gcttcaccgc ctttgactaa gatgccccgt
    10861 ttcgctccat taccgattcc tgctacattc gaaacaggcg caccaatgac taaagcgcca
    10921 ggacaaccaa gaaccaaaac agtgattgct aaacgaaaat cacgactaat cagaccaaca
    10981 accaacgcaa taagtaaaac ggttggagta taatactgag aaaatcgatc aatgaatttt
    11041 tccgcatggg atttggtatc ttgtgcttct tctactaatt cgataatttt ggcaaaggtc
    11101 gtatcatctc cgaccttagt tgcttcaatc gtcacgtagc catctgctaa aatcgttcca
    11161 gagaagactt catctccact gattttgacg acttgtcgtg attcaccagt cactgcggct
    11221 tcatccgcat accctttccc gtcaatgact cgtccgtcca caggaatagc tccaccagtt
    11281 tttactaaga cgatatcgcc ttcttccacg tcatcgattt ccacttcttc agtcgttcca
    11341 tcttcttgga caacactagc agtagatggg gccatctcgg tcaaagcctt gattgactcc
    11401 cgcgttttgg tcaatgtttt ttgttccaga tagctaccaa acaaaaacaa gaatgtaacg
    11461 atagctgact ccttgtattc tccgatcata aaggcaccaa gcacagcaat agtaacgagt
    11521 aaatcaattg agataacttt gactttcact gcctgatacg catggatcat aataggtatg
    11581 accgcaataa tagaagctat ggctagagca atgttataga acaagacaaa accagtaaat
    11641 tttccgataa aaccaaaaat aatcaacaca gctgataagg cggtgatatg atttcgatgc
    11701 tcttggatcc acttttgtaa tttcatcttt ctccctcttt tcctttcatt cttacgagac
    11761 taagtataga tacttatcca tcataaaaaa ttgaccacta tcaagtttag tcaaaaaggc
    11821 ggttttcttt tggtagaata cactacaaaa aatagttagc aagaagactc actaaaagtg
    11881 atcttgccaa ctattgtcgt ttctatcgaa attttcattg aatgattttc ctgtacagca
    11941 tacatcggag atctatttct ttatctaaga tttttatctt gctaattttt tcctttagtc
    12001 tttcaaatcc acgtcgttca tagaattgat acgtacagcc actatctgta aaaaggaata
    12061 tttcttttcc tttctctctt ttttcaaatt ctgttagcaa cttggtaccg atgccttttg
    12121 ttttcaaatc aggatttgca gctaaaaaaa tgatctctcc atctggcgaa tgcttcttta
    12181 aatatgctgc aagcatttgt tcatttgctg tctcgtaagc tcctttactt tctttagcaa
    12241 atatattttg taaaaaatcg aatatcttaa catatatttg ctgtccaaaa gaacgatgtt
    12301 tctttttttc tccttttatc tccgccagca aaacacctgc caactcgtcc tctgcataca
    12361 aagcaagaat ttgagttgct cgattcaact ccatatacaa aaaatatctt ccgtaaagat
    12421 tcagcagaag ttcattttcc atatatctct caaagttcat tccttcaaca gcaaaataga
    12481 tagctttttt gtaatctttc ttttctaagg gtttaaaagt tagtttcatc atttcgcctc
    12541 ctcaacttca atgattgtgt tatacttaca ttataataga cacagtgtct actatctata
    12601 atcaatcatc tataagtttg tcgattaagc gacagctttg ctttgatatg tcggataagg
    12661 agataggtaa atgaaaaaga cagatagacg cgtcaaaaaa acagaaaaag ctttggctga
    12721 aacactatcc acattattag taaataaaaa gattcaagca atcactatac gcgaactaac
    12781 agaaacggca gatgtacatc gttcgacttt ttatacacat tataaggata tctatgatct
    12841 atatgatcaa ttagaaagca acttttttcg ggatctaaat gaaattctct cttatgatcc
    12901 agcccatagc tatgaggaac tatacactcg cttgattgac tatgtttacg caaatacttt
    12961 tttatatcgc ttatttatcc tcaaccatgg tgatatttcg tttcagaaga aagttaaaca
    13021 agttattgaa gaaaactatc tgaacatatg gctatatgaa gacaagaaaa cgattatcac
    13081 agaagaaatg agattcttta ctacgtacca cgtccaaggt tgtctatccg ttctcaatcg
    13141 gtgggtagaa agcaattaca agtactccaa aaaagaatta ttagaactct tacagaaact
    13201 gaatgatcac atagagaaaa tcatgccttg aataaaaaca tactaagctt agttaagaag
    13261 aaccgtaacc ttctcgtata acaactgaat cttatttagc caaattgttt actgaagatt
    13321 ttttgagtaa aatttctata attcctactt attgaaaagt agtttgtagt gctgcttctc
    13381 cctctctaag tgatatatct tgttgtacca atcaaattca cctcatcatt aataaattac
    13441 ttttaggcta atttattaca acttataggt tgaagctgta accaatttat agtcatatta
    13501 tatttttatc atccagttta ttgaattctt ctcgtttacc tcaattcatt ttcaagggga
    13561 atcagctcat catctaacat tttacgccat gctcgcttca tttcttgtgg gtatttaaat
    13621 ggtttcgctt ctggatgttt ggctttatat ccttcgtaat ccttactgtt gataattgct
    13681 ttcatattag gaattggatc ttcacctggc atatattgca gattagaagc atgtaatttt
    13741 ttctcagaaa caaaccactc attggaaaag acatgaatca aatcatcact tacttgtgta
    13801 aaatagcgac gtttaatgac aaagatatct tcattgctat ctagtgtacc ttcatttctt
    13861 tgtgcttaaa ttcaaagata attttctcaa taaagatttt tgattccagt aagattcctt
    13921 tactcctttt acaacatagc gttgtccttt gttttgaaac atcttcttcc cctctttgcc
    13981 attaaaaata gcccaattct ctataaagaa gattaagctt cattattata atataaagtt
    14041 taaatttggt ttattactta ctttttctta ttctctagta agctaatatt gaaaaaaatg
    14101 taatcagatc attattttta tatgttgtaa cttcctgata agaaaactaa ttaaaagaaa
    14161 gaggtaacta cataaactaa ttaaaaaagc taacttcata gttggaggat aaatggaaga
    14221 aacttcaatg attgtttctc cttgtgttcc ttcaatcgac actagtccta ttatcaatct
    14281 ctcttttttg tccatttact tttgcgactg ttccccaata atcaaataca ggaagcgtta
    14341 ttttttgtcc tttagattca tgtactgtat agactatttt cgaatccatc acttgataag
    14401 gcatagtatg ccattccttg ccaattctta cttcatgatt ctgcaaatgt tctttgtatt
    14461 gtaatgcttc cgtgggtgca tagtcataaa gtcctccggg aaggagtgtc gtgtaacgaa
    14521 taagattttc ttgattcatt accaagtgat ctctctgagt gacaacttta tgttcatagt
    14581 ttgcttttgt tgcagcagat acagccaaca gtaataaaac aattgccgtt atattaacta
    14641 cttttctttg cttagctgat gtgctcttca tgaaaacgaa cgataaaatc aacgagccaa
    14701 acagtacttg aaagcctaag attcgccaag gaaattgaat catttgaatg ggtgtatttt
    14761 gtaataaaga ccatggaaaa aatttggtgg ataagagcca agagactacc gcactaaggt
    14821 aaatcacttt ttctttgcga ttaaatcctt taaaaaagaa gagtggacag atcaaagaaa
    14881 taataccaat gattcctacc gtaaaggttg tcaggtcatt attcatcgca ccaatcaaac
    14941 tatccgagac atttaatgct tggtgttgta aggtcgtttt atctggctca ttaatcgatt
    15001 gaaccatgat ttgtcggaac atcggatacc aaaagtatgc tgttaaaaga atcgtcattc
    15061 ctactgcctt tgacaactcc acaatcaacc ggatgctttc tttttttctt gtttcactta
    15121 ctacaaagcc gcctaagact agtaagccaa cggctattac actcataaaa gtggaaagaa
    15181 catgtgtata aatgagtaag ctcatactga ctgctaatgg aattgcacat ggctttttct
    15241 cataaatcag tgcgtaagca taatacataa ctatcggtat aaccgtaagt gcaatcgctt
    15301 cagcaatagc agaacgatga taaatatcaa tcgtacggta cccataaaat gtatataaac
    15361 agctaaatat aaaagccgca tcgattcttt ttaaaaattt tagtccataa tgaaacatca
    15421 gcatgatagt acaaacattc aatagccaaa catataagat ataactaacg attaaattat
    15481 tacttatcca ataaaaaatg actgccggaa aaaaggtcaa gtagggataa aaataattca
    15541 ctccgctccc ataatgatta aacgtcgtaa aattgattgg accagaaaaa attgaagata
    15601 accctttgac tctagcaaaa tggaaggcaa tatcccccga tgtagaaata gtatgaacat
    15661 ttctaaaata attgattgaa taaagtccag caataattgc taggagaatg gacaatatta
    15721 tttttttctt tacttgcatt acttaattat atactccttt catggattgc tttatctagt
    15781 ataacttaaa ttttaaataa atcgttattt atttttactc tcacttaaaa aagcactaaa
    15841 attataagag agggaggtaa tggtctgaac accaagaatt gttccaacat cgaattgctt
    15901 aactaatgag ttcaaagctt aattatgtgg ctcttatatt taccgagcgt ggattgcgat
    15961 ctctttagcg aaacaaacat ctttcataaa aaaaacagcc atatccgctt tcttggatat
    16021 gactatttac tactacttta ctatctttca ttcttcttgt ctcacttatg ataaggcgct
    16081 gcgtatcatg agtgaatgat attaggaaaa cttcataaaa taaatctttt atacatttcc
    16141 acttctagat ggtcattcat gttagaagaa tcaaactatt tttttactgt cgtaataata
    16201 ttaaaacact agaacaaatc aattaaattc cataaaatat aaataataga cctagcatca
    16261 tctggtataa aatggatgct aggtccgtta tatttactca aaattgattc tacgaataga
    16321 tcttcttatc atttctattt tagtagtcat gcgttgttgt tcttctaaag gtggtaatgg
    16381 tataagtagt ttagataaat ttgtaccatt tacatttggt tgcccagttc cgtaagaaat
    16441 tttttctaat aacttccaat ataatggcga gtctaagaag cagtctacgt attctgataa
    16501 taatttctca actaacctaa ttcgaatcaa gtaagaagca aaaacagatt cctcacttat
    16561 ttccttaacc aagaaacttt ttcccatagt tcctcctgtc cttgctatca aaatgtcatt
    16621 ttcttccaat cttaaatcaa ctagtttaga attactaata tcacagtaag gcacactgga
    16681 ccaatttaca cgtccctctt gtatatctgt gattctcaaa aacttaacat tgccttgttt
    16741 tttcgccgaa tctgtatagc catattgaat cttagtagaa atacttccta aataaaccca
    16801 tagtcgaaca tcgctagatg atttttcttg tcgttcttta tgaacaacag tgatcatctt
    16861 gagttgtccg actatttttt agttgcagca atctttttgg gacgcttatt tgcatgaagc
    16921 aacgttgcta agtctagtaa taaaacattg gctaacacag gattggcttt ctcgcgatac
    16981 gctgcggcgc cactttcaaa caatactgct agacgatctt ctacaacaac gatttgttcc
    17041 aaatacggtg gaaatttgat tcgttttagg atccggctag gagagtcccg ctcggacact
    17101 aagagttccg aggtataagg accataagaa cgactggcta agagataatg gccataaaaa
    17161 gccacacctt gaatcttatc agatgttttt ctgctcattg tcacggttgg ttggattttg
    17221 tctttgcggc gaattgctgt tttcatttca gctatcgtcg gcaatagata gaaagtcaac
    17281 tgtccttcac cttttagtgt gaagttgcca gataccagtt gatcattgtt ttgagtcaaa
    17341 tacgatgctt gtggaatctc tgagagatca gttgtcagcc aataagcgat tggctgtccc
    17401 gtctcttcag atgtatctgc taagatatcg atcaaacgta aagcggctac tcgtccagta
    17461 gcagatcctg tgattgtcaa ccataaaagt tcccgttttg tatcatatac taagccccct
    17521 acatgtggcc gtttaggtaa aatcagcagt ttgatcggct gtcctgtttc tctatccagt
    17581 acatgaataa tagaatgatg ctgatggtca tagcagtagg cagaaataaa cacaaaatct
    17641 tgagaaaaag ccaacccttg cggcgtcatc cattgacaaa cctcattttc gccggtttcc
    17701 ggcaccacca ttcttgtttc tgttaatcca ggaatgttat attgttttgc ttcagccttt
    17761 tcccaagctg cttgaacaat aggataccgt tttaactttt tttcaatatc ctgatgtatc
    17821 cgttgctgct tccaatgctt aaactttagg acatagtaaa caagaaggcc aatgaccagc
    17881 aacactaaaa tcattattgc tacccgctgt gtcatttttt tgcctccttg ttctattcga
    17941 tcaatcgtcc atataccgct attaattacc gtaactacat gtttctttta ctagcgtttc
    18001 tttataggat ctttcataat ctcatttata gtaacattaa gaatccctta ttctactata
    18061 gtcacttcca catcaatatt gcctcttgtt gctttagagt atgggcaaaa tgcgtgggct
    18121 tcttctgcga ttttctctgc ttggtccttc tcaagtcctg gcaaagaaac ttctaaaacg
    18181 accccgattt ggtagccccc atcttctaat tgaaataaag atacccgtgc tttgacttca
    18241 gaatcagact caagattatt ttcttccagc atgtgttgaa ccgcgccgtt gaagcaagat
    18301 gcgtatccag ccgcaaaaag ttgttccgga tttgtccctt cactatggat tcctgggcgg
    18361 tcgatcttca tgtgcatact tccgtttggt gcctcaactt cgccatcacg gcctccacga
    18421 ttgatcattg ctgtttcgta catttttttc attgagatca tcctttcttt cgtcgttcta
    18481 tattaagtgt aacgagtatt ttttaatctg acaaaggtaa tgcttgcatt cggcgacatc
    18541 agttagacaa catcgatgat cagtgataga agcagtttat ctatacaaaa aaagaagggt
    18601 tctatgatat ttggtgtcct tactcgcctt gagtaacaac accaaatatc atagaaccac
    18661 ttaatcagct tcacactgct ttttctactt tcgtagttga aaaaaactta aacaaatcat
    18721 cggcaaatag ttaaagaaaa aacttaatcg ttgaaagaag cccaattgct taaagaaggc
    18781 aaactgacta atcgacggaa tccgtgctaa tccgtaaaga cctgcaatta gtaaactaat
    18841 aactgttaaa acaagataaa aatggctaag cgtaccttga ccactttgac gataaagcag
    18901 aacaagtaac aatgggaaaa gtagaaagcc tgcatagcct agcccagaac ctatattatg
    18961 gatccaagtc gatagattac aagagctttc attcgtattg atactgaata agccagtaaa
    19021 aatacaatca cctatcccat ataaaccaac tgttgcaaca acaagtattg caagtgtttt
    19081 tgatgttcca acaaataggt gatacaacgc aggcaacgaa agaacaaaaa aaagtccaga
    19141 tacaactgac cagaccaaga aagcacgtct gactggacta tctacgtcac caaacacact
    19201 aattacggta gtcatttgat tcagtttggg ataaaaaagg ccaaggatat acggtgtcga
    19261 taaatcactc atcacaccta ataataaaaa ataaaaacca tatttttgca aaaaaatcat
    19321 tttcttcccc tactttttag tgattattcg atacatccaa caatcaaatt ttttctcatt
    19381 ttaacacata tttttattct agttgtcttt tgaacaatca ataacataaa ggtgagtttc
    19441 ttctacattt taaaaaatcg aatggcactg aagagttcag ttgtcagcat caatctccta
    19501 ctcaaaaagg caaaaaaacg taagtcagat gttgttgaca tctcacttac gcatccattg
    19561 attgaaaatt tcttagtaaa atctactagc ctaaaaattt gaagacttct tctcgatcat
    19621 acagtttaaa tactttctcg cataaaaatt gagtaattag tgccgcaaaa acagggatga
    19681 ctaaccaaca aagaacaaca agaaagccat ttagccctgc atctaaagaa gccaacggac
    19741 caacaagtcc gaccacgccg aaaccagcag aagcaggcgt acctgaaata gaaaatagag
    19801 cgacaggaat agctgtaatc actgcactaa ctaaacaagg aataagaatc acaggttttc
    19861 taaatagatt aggcatcatc atcttcatag cgcctaaagc aatcgcaatt gtcacacctg
    19921 acttattaac cttccaagag ttcactacta aaacaaaagt ggttgcagct acgcccatcg
    19981 cagccgcacc agcagaaaca ccatctagtt ggattgccat tccgattgct actgttgaaa
    20041 taggagagat aatcaaaaag gcaaaggaac aaccaatcaa gatagacatt aacagcggtt
    20101 gtaaggtagt gaagttatta atcaatgatc caattgcaga agtaattgct gttacaaaag
    20161 ggaataaata aaaaccaagt aaacctgcac ctacacctac aacaatcggt gttaacacaa
    20221 tcgacactga cccaaaacta tttccaatca gtaaaatcag cccgacagca atcgatgctg
    20281 tcagcatcgt attaataata tcgcctgtac cagcaccaac gtaaacaccc gtttcaccca
    20341 aatcaggtgt gaatttgact acaccagaac caacaaaaga agctccggct acaaccatca
    20401 ttctcatcgg atcaaatcca aattgtaagg caatcaaacc accaatgatc aaaggtgttg
    20461 ctagttgaaa aataactgct aactgtgcga tcgttaccgc aaagggatac gcactaaaat
    20521 attttaaaat ggcacctaat actgcattag gaatcaatcc aataatgatt cctgttgctg
    20581 taccagcgag gactttattc agaaaaattt tcggtgatat tttttgtgaa gtttccattc
    20641 aatctctcct ttattttacg ttcaaactat cttcttttgc atggacaagc tcagtcaaga
    20701 aatcacaata aggcgtcgca acaccatatt tcttcccttt tctagaaatt gcgccattga
    20761 tataatcgat ttctgttaat cgatggttct taatcaagtc ttgatacatt gaaggataat
    20821 gtaacccaat tgtttccggg tcaaaacaag attcacaatg tgcaatgact tctgggacat
    20881 ctagttcaat cttctctact gctgctactt tggcaaactc attgacaatc gtcgccacca
    20941 ttttatgagc agtcgatgtt tttcctagct cagccatatt aacgtctaag attgtgcata
    21001 gtccgttcat tgttccgttc acacatgctt tgcgataaat agaatagtga atattgtcag
    21061 aaaaatgagc atttaatcct gattcagaca atttgtctgc taattttttt gcagctgctt
    21121 cttttccatc acctaaattt tgtaattcta ctgaaccact tccaaataat ttgacctgac
    21181 caggaccttc aagacctgcc gtccacatgg tatttccaat atagatattt tccattggta
    21241 caaatttctc aataatatct tcatgcccga taccatttaa tagacataag acctctgtat
    21301 cttttttgat taatgattgg atatcctgca gcattttttc cagctgcatc gctttggtaa
    21361 atagaataat cagatcaact tgatcttctt tttctacttc ggattgaaga acgattggta
    21421 gttttgcttc tacctcttta ccattaaaat ttgcttgtaa tccatgttct ttgatttgtt
    21481 ggacatgttc tgcccagcca tcgattaata atacttcgtt cccactttgg tgaagcatca
    21541 acccaaagcg acttcccatt gcgccagcgc ctgcaattgc tattttcatt gattcttcct
    21601 cctttttctt tctattatac tcatttgaga atcttttcac aacctcgttt tgttattctt
    21661 ctcttattta tcatgtttat atccaccatt caagttattt tatcagcaaa tcaataaaaa
    21721 ctggaataga atcccaattt catcgtaaga gaaactagac tagcaataag aaaatagaaa
    21781 cattcataga aaaaatctca ctcctctgat tataaaagag ggggcattaa cctcgtgtat
    21841 actaaaacct tccattcatt cgatggatga ttttcttttt gtgaaaaaat attcattgta
    21901 gtacgatgtt gcgcaacaca gacggatggt attgcttatt atttattaaa atcgaacaat
    21961 acacttattt aaaaacttct ttcccagatt ttcttccatt tttacagtta taactagtat
    22021 tatcatgaaa aatagccttt tattgtctat gggaataata atttaaaatt ggtacttttt
    22081 tattaattag ttcatcgccc aataatctct ccagtttctt agcatctcct gactcaaccg
    22141 gaattatcca agcccagtct aaataatttt ttaaaatctc atcatatttt gcaaatattt
    22201 cattgccata ttccgtcctt aaaattatat tttcagtgcc agacaagtga tttatattga
    22261 gttgataaaa aagatctcca tctaaaaaaa tatcattaga agaagatttc cccacagtaa
    22321 tgaccattcc atcagctcta tgtgttacat acaaataatt gtatgacctc atttcaccta
    22381 atgaatcaat tatttcttgc cctttagctc taatatattg atctctatta cctatctcac
    22441 tcttatttaa caaaagtttt ttcatgtcaa atttaatcgg ctgttttatt tttgtaaatt
    22501 cgcttctact actaatccaa tccgagatta atttttcaac atgtcccaga gtaaactctg
    22561 atttattcgc taaatacttt ttttgttgta ttaaagacat ctttccccga agctcaaaat
    22621 tgagcctaca tcgattctct atctcttcaa aaagcatctt atcttcatca ttcttatatt
    22681 catcattcat aaaaccaatc cagctcctcg attaagataa tattttaatc tcatacaaaa
    22741 actagctctc atcaaaacaa ccattgtttt gatttctcat gaaatttctt tatacttttc
    22801 tccgattata aattcctaac cccttgagaa aattacgcat atatttatct ccgcaaggct
    22861 taaaatttcg ttggccttct tttctcaaaa atgccccgat ttcgcctttt gatggataga
    22921 tttctgcctc agcaaaaacg tctaaaatat catcagtggt catagacaag gcgattttta
    22981 atttttttag caacacattg ttgatatatt tgatttcctt gcttttttgc ggcgtgtcta
    23041 ctatcggctt atctttttgc ggaccacgcg ccaagagaat caggccattt aggaatgcgt
    23101 caaaaacttg gttgttgatg gttttttcat acacattatt ttctgcatta tctttgttct
    23161 tatcctgtac tttggtcagt accgcctgag cttcttcttt agtcagtggt acaccgccta
    23221 gctcaaaagc tttaatcaaa tccgtatctt tcaaatcaat cgcataacgc aatcgcaata
    23281 acctatcatt gttattcatt tgttcctccg aagtatttag tctttacttt cttacgtact
    23341 ttttgtcttg ctaagatttt taggatcaat gataaaaacc gcatcactta tgataaggcg
    23401 ctgggtatca tgagtgaatg atattgctga atacaaatag tccgaccaca ttaagttgat
    23461 cggacttcat aagcatttta ttgactaata cttttttgct taagaattga ttctaacatc
    23521 ttttgctaaa gttaagcatc ctatagttcc tgcattatct gctaacttag gtgtgacaat
    23581 gtattcttcg atatttggcg tatcgacata gccgttaatt agttctaaaa aacgtctttg
    23641 tactttcttt ttcagatgtt cttgttgcat gacaccacca ccaaaaatta taatatctgg
    23701 tgaaagcatc agggtcgtat tacaggcaca ttgtgcaata taatccgcct cgatttccca
    23761 gaacggatct tctatagaaa gatcttgtcc cttggttccc gtacgcatct cgatagcggg
    23821 tccagatgcc aacccttcga gacaatcatg atgaaaagga caagtaccag agaaagtatc
    23881 tcctgaatgt ctcttaacta acgtatgccc catttctgga tggctaaacc cttcaacaaa
    23941 aatcccatct tgtatagctc ctccaccaat tccagttcca acagtgtaat aaacaacact
    24001 ggataaacct tttccatgac ctgaaacata ctccccgtaa caagcagcat tcacatcggt
    24061 tgtccaggca attggaattt cgaaatgctt attcaacgtt ccaacaaaat caaaattttt
    24121 ccaggcagtt tttggagtag aggtgatatg tccataggtc tttgattcac gatggatatc
    24181 aatcggtcca aatgacccta ctccaatgct ttctaattgt tctttatagt gattaaagaa
    24241 atcaattacc aaactcattg tttcattcgg tgtagttgta ggaaaactca ctctttctaa
    24301 aacctttaaa tcatcattgc caatggcaca aacaaattta gtcccacctg cttctattga
    24361 gccatacatt tcttactcca ccgtcctttt atctaggaat tcgttcttcc ctgtctaata
    24421 gtttttcgaa aaactcatgt ggttcctttt ggtaccaata ggcaacagaa gaaatatcgt
    24481 cttgacgctc ataaagcttg atgtcatcat ttcctagggt ttggaaagta acacgtaaat
    24541 tttctttgaa actaatgggg tcgggtaaat gccagcggta caacccatgc atcggtaaag
    24601 catcatcgcc aaaaccatga actggatttg gattaggttt tccagcacta aaataatccc
    24661 gggtgtgatc tcttttagat tgaaaaggat agcccataaa tagcgtatta aaattttgag
    24721 catatgccct ttctccaaag ttctttttat ggaaagccca agcgccgcca aaataatctt
    24781 ctgctcctgt agatgttatt gtagggaaat cttcatctcc atctatataa aacttaaatt
    24841 ctccttcccc ccaccagtat ctttccaatg cgcacaaagc aaagtaggtt cctacataat
    24901 acccagtgcc tttgattcca tcaataaggg tgtaatcttt tccttttgtc gtgatgggtt
    24961 cacggttcca tttagcatga aaatacatcg catcatccgg tacttcagtc aatgcataat
    25021 taaatgtata aaaaatgtgc ttaataaact caggatgttc attcgttaaa gtaatcttag
    25081 ctttttttcg aaatggcatc tcaaaatagc aattcatccc tgatgttgga ttcactacga
    25141 ttggtaagga attcacttcg catcttgtcc caaacccatt acaaaaaaaa tctcctaatg
    25201 gactttctac agaaggagtg gattcatcat cccaataaat acgcaaaatt aaatcacgta
    25261 agacgaaact cccagcgatt gttttgtctg tcactgtcat ccaaaaatga cgtaaaattc
    25321 cagggccttc catttctgct aaagtaattg tctctccagc atttatcgga acagaaggtg
    25381 acccttttct cccaattcct agatgactcg ctgtcattgc cgctttccct ttttcaccat
    25441 ttttattttc tggtgaaata gttctacttt cctctccctt gtggagccat atattattta
    25501 aacaattcat tttattcgtc ctctatcctt tcttcacttt tttgattatt catatagatg
    25561 atttgttgtt gtgcctccgc aataattgct ccaatatcta aattatcatt tcctaactgc
    25621 aaagcgattg acattgccaa gggaagattc attcctgcaa ttacgtgcac tctgtcagaa
    25681 atataggtaa aaaacttttg atttacactt cctccagcca tgtcagttaa aacaattatt
    25741 tcgtctgtag tatccactaa ctctttaatt tgttcatcta tatcatcaat ttcttcgaca
    25801 tatgcacata agctactaat ttgattatgc atacctgtta aaaactctag tgtctcttta
    25861 aaaccctcag ctagtattga atggctagca atgatcaatc ttctcataat tcaaatcctt
    25921 ttcctttacg ctaatattcc aaatgcagcc ataaccatcg caaaaacaat aacgagtaga
    25981 atcaaaatgg tcatactgat ttttttctta ctgagtagat aatatgctgc agcagtaata
    26041 attactggaa ttaatgttgg taaaattttg tccaacattg gttggatttt catactaact
    26101 tcaccaaaag tgaatttaag tggcgttgaa acactgataa ctgaagcaat taatgatcca
    26161 actactgtta gccccaatac tgaagcagct tcagttaaat aattaatctt ttcaccgtat
    26221 tcagtaacaa tcttcattcc ctgcttataa ccaaattcaa agcctctcat tcggaaaaag
    26281 aagattacca tgattaaaat gaaccataaa aagattccta caggattatt ttctaaagcc
    26341 atataagccg caattgatcc catgatggtt gggatcatcg tcatcaaaag agagtcccca
    26401 ataccagcaa ttggacccat cgtaccaact ttaaagtcat taatcgcatc ttttgcttct
    26461 aatccttgct tttcctccat tgcaacacaa gcacctaaaa taatggctgc cagccaaggc
    26521 tgtgtattgt aatactgaaa gtgattattt aaagcttcga tgtattcatc atcatctttg
    26581 tataattttc ttaaaatagg tgacaatgcg taagtcacag atgcaccttg ctgtgtttga
    26641 taattaaagg tacaaactga catcaaccaa cgatagttcg ctttcctaag atcctttttc
    26701 gttacttgat atttttcgct cattttattc atcctcccca ataaacccaa catcagaagc
    26761 tcccgcagaa gcagtgattt gtgcatcttt ttcagctcta tgatacacat taaatgctaa
    26821 agcgaatcct atgatcgccg ctccaataat aggtaccttc aaaaaagcgg ctaagaaaaa
    26881 tccaacaagt aaataactaa tgtatcgacc tgtaggcata aagcgtaaca acatcgcaat
    26941 accaacagca ggcaacattt ttccggctaa tgttagccca tccataaacc aattaggtaa
    27001 aacatccaaa acgtaattaa cagcagactg tcctaaagta acggcaataa atactggaat
    27061 tgctgcagat aaaccaaaga aaattggccc caaccacaaa acactattca ttttcttaaa
    27121 ttctcgttta ttcgcatatt cctgtgattt gcgaacaaca aaaccattta agattttgac
    27181 aattacatct agttgcaccc ctaacatacc tacaggtagt cctattgcta atccggcttc
    27241 tactccaagc tggctaccaa atgccgttgc aataattgcg cacgttccat agtccggtac
    27301 agatgagcca ccaaaaccgg caactcctaa tgacatcaat tgaactgtac cgccaactat
    27361 caaacctgtt tttatatcac ccatcaaaat tcctgtgaat aatccccaaa aaactgcgta
    27421 atttacaaag atcatcggaa tcccataata atcaacatgt ttaataaatg ctaatatagt
    27481 cagcataata atttgaaaaa tagtaaaatg cattatcctt actcctttat cctaattttg
    27541 attgaatttc ctgtgcctta tccgccggaa caaattgtgt aacaatttct acaccagaat
    27601 ccaacaattt tttatacgtg ctgatatttt catctgatgc ataggctcta gaagatagct
    27661 gtaatgcatt atctttaaca acagtaccgc caacaattag tcggtcaatt ctaataccgt
    27721 tatctactaa atgaaggatt gtttccggct ctttcacgat gacaaatact ttttcctgat
    27781 cgtatttccc ctttttaaaa ttcgcaatcg ctgtttcttc agtgataatt gatacagcca
    27841 ttccagcagg tctagctaaa cgcatacttg atttttggat gtcgttattt gcgacagaat
    27901 catcgatcac catcactctg tttgctttag acattggtgc ccattgcgtg acaataatcc
    27961 catgcaataa acggttatca actctcgaca taacaatact cataaatact tcctccttta
    28021 tctttcattt tcttacaagc aattaccatg ccaaaacaaa aagaacttga taaatcaagc
    28081 tctttttaca taatcataaa cgtatttaac ctcatgaatc gggatgtcga cactataata
    28141 atgttcaacg acactgaaag attgcttaaa atgtgtctga aatttttgaa attcctcact
    28201 cgtttcattg aaataaattt catcttccaa agtattttgt tttactaatc gttcaattaa
    28261 gcaactaatg tgaacatata atccgaaaca cgtattatta tcaaatgaaa ttcctagatc
    28321 attttgtaag atgtatatag catctgaaac ccgttctaat aatttagtcg gatttaatat
    28381 ggtcaattgc tccattaaat tcgttagact aaaattcttc agaatattat tgttcaaaac
    28441 tttcagttca ttatcattaa agtgtctatt caattgattg gagaatttac ttatattcat
    28501 tcctaaaatc atatcttcaa ttgaaaagaa ttctacatct tctagatgtg gatttagtgt
    28561 tccaacaata aaaagaatat tgttctcttt acgaagctcc gataaatagt tttcattcac
    28621 taaagagtaa tagtcacacg tttctatgac aagatcagta tcccttggaa agctatcttc
    28681 aagtatcttt tttaatttaa acgaagttcc taatccgctt gcacaagaac agacaatcat
    28741 atccttctta atatttgatg attcataaat ataatccaca ttttgatttt cttttacttg
    28801 ttcaagaatc tgtttaagcg gtaaatctaa taatatctga tttgcaacat caatagccat
    28861 tcttgttgaa acattgttta gtagtccgaa ttttttttga gcgtctagtt ccaaatttag
    28921 ataaattttc tccagtgaac ccatatcaac aagtaaaata atttcttcat atccggtaca
    28981 ctcttgtaag tatctattaa ttttgtctgc aatctcacta cttgaagtca ttagtggcat
    29041 atcaatggcg tcgtatatat atctctcaac gatcctatta gcagtacttg ctatacaggt
    29101 tgccgttaga tctccatgag ccaaaatcag tgctaaacgt ttttgctgaa attctttact
    29161 acacattaac ttaaataaaa taaccaaaac atttttttga atttttgtta aataaatatc
    29221 ccctatttga ataaaacgat tgactaattg aacttctcga ctacaccaac tattaatatc
    29281 attgatgaag gaatataatt tatcgtccga tgttaattgg atttgataaa tccaagaatt
    29341 caaaaagata gacgttattt gaagataata atagtcaaat tctatttgaa aatctctctc
    29401 tatttcctta acatactgtc taaatcgaat ctcttgatag gtgggatatg ttaaagctga
    29461 attattttgg atatcaacat tcttttgtct aaaatatcta tcatatttct caaaaagctg
    29521 ctgcttatca gtgtctttct tccacgtttg ataaagatac tctataagtt cgttgatatt
    29581 aaaaacttcg tctacttttt ccaaatcatc catttgaata tattttaatt cattctgtct
    29641 tttcataacc ttcatgccgt tgaaattcac ccattgtggt aaatgataca tctgaatata
    29701 aagttcttcc aaatcatcta agaaataaaa ggcattggca caggtcaatt gaatgatatt
    29761 tttcaaattg ccaacatttt cactaaagcg atagtttaat aaaaaatcta ataattgttg
    29821 agatatcagt attttctttg ctagtctttc ttcttctgat ttaaaaaata tcttgattaa
    29881 ctgttttctt tcataaagag aacgactgtc taatgttgga agtgttagct ttacaggtat
    29941 tcttctcaac aaagttgata atagtttatc ctcaggtggt tctgatgttg caaatataat
    30001 ccagcaactt gatgtgtacc agttttcatt atcacccatc cgatgaaatt tcctcgtatc
    30061 cataaactga aaaagtttct cctgacattc attgctcaat gcatgaattt catctaaaaa
    30121 taagactcca ttattagcta attctgctaa accctttctt ccatcttcgg ctcctgtata
    30181 tgcacctttt acatgcccaa aaaaattgga caagaaaagt tctggattat tggcatattc
    30241 actacaatta actgaaatga aagatgcctt ctcttctata taattattgg ccaacaaatc
    30301 cttatacaca taatttgcta aatagctttt tccagttcca gtctcgccat gaagtaaaat
    30361 gggtaagcca tttggcggat agtagattgc tgctttaatt tgagcaatta catttcgcag
    30421 acttccttga cttccgataa ccctttctat tccaacccta aaagagctat cataccattt
    30481 ttccgcttcc tctgaagagc taaaaatttg ttgatttgga actttctccg gtaaagaaaa
    30541 gaagattact ggacgagtat tgatttttcc aaattctcca tcttttacga attcattcaa
    30601 atattgagag acaatattac gactaacatt taatttttca gatattttct gtgctgtatt
    30661 gttcccccat ttatttttaa tctcagagtt ctctatgaca taattgacaa tgcgttcttt
    30721 taattcttcc atcacagcac ctcctcgaca ctattcgaat tttgccgaca gtatttttcc
    30781 ataaatatga cttgctaaaa aggctttgtt tagatcattc aattggaacg ttgcttgttt
    30841 tgcttgatcc gctaagacgt tgaaaagata ctctggcgaa atcgcatacc ccaaaatatc
    30901 aacgattgtt gcaatcagat cttcttttga ttcttcatct gaaagtaatt cacgatacaa
    30961 catcgtttgt ttgcttactg tgtcatattc ctctaaggat tcatctgcta aaagaaccac
    31021 ttgttccaac aaacggtcgg ataaatattt atagaaaatc agccccaata aataattttt
    31081 atattctgac gcatccattt tactacggag attatctgcc gcactaaata acttctgatt
    31141 taattctgca cccaaaaaga tacactccta tctcttattt aaagttgcta tataccctct
    31201 tattttagca tactcagttt tagaaaaagc aaaaaaataa agaaacttat tcttgataac
    31261 aagctacgtg acgtatatcc ctaacacaaa aatagccata tcctttggtt tggatacggc
    31321 tatttgatat tactcattca attatcaaat cacagtaggt gtgaatctca tcgggatcgg
    31381 caatatctac ttctgattag gatcaaaggt gattttctca ataaaggtgt gatacaaagc
    31441 tttaacttgc ttcttctcac tctggataag aaagcaatcc aaactagtca atatctgttg
    31501 gacatctttg acttgaatcg gttccccttg ttgttgaaag atccctaaga tttcattctc
    31561 tcgaatttgt aactgtcttc gtttttcagt caattcattt aaacgatctt ttaacatcgg
    31621 aaacaattcg ggactatctt ccaaagcagc ttcctgtttt tgctctaata gttagatttt
    31681 gcgtaccatt ttcttctttt attttccttc gattgctgat tttaatatag tctaaaggaa
    31741 atttatattt attgtattct ttataacttc aagagaactt cgtttgagta ttactgtatt
    31801 tttttaattc cataagaaaa ttagttatta cctgccacct aatcagcaaa tctgaacctg
    31861 agtcaatata attaatatta ttttccatct ccaacattgt aagtactgtt ggtgttaata
    31921 tagggacttt gtacaattgt atttttttga ttttatcatc tgcacaaagg agatttttta
    31981 aatctaatcc ttcttttatt tcaaaagttg aattttttaa atctataaga gtaaaaccaa
    32041 ttccttcata ctgaatttct gaaaatcgaa tttttctccc agttgataga tcaaggggca
    32101 gaaaaccttt tattacattg tgagtgtcac catcatttac aatgtcatta ggaattttga
    32161 ttttagaatt ctgtggagca cctattggaa gataataatt cattataatt ttgtagtctt
    32221 catgaggaaa taaagtactt ttaggattgc taagatattc tcgtaccgaa tctaagaatg
    32281 gcttatccat tgaatcttca tccccattta aactaaaaaa cattcctaca attgcttttg
    32341 caactctaag tgaattcatt ttctttattg aatgtgttaa atcagactta ccattcttta
    32401 aacttgtatt gttataaagg aaatttttat tgtcttctgc aaatctaata tattcctcaa
    32461 catactcact accaaaaaaa ctattacatt tactacataa cg
//
LOCUS       DARBEC010000022        32183 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.022, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000022 DARBEC010000000
VERSION     DARBEC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32183
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.022"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(121..501)
                     /gene="yajC"
                     /locus_tag="SCL80_000992"
     CDS             complement(121..501)
                     /gene="yajC"
                     /locus_tag="SCL80_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEG1106878.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(598..1743)
                     /gene="tgt"
                     /locus_tag="SCL80_000993"
     CDS             complement(598..1743)
                     /gene="tgt"
                     /locus_tag="SCL80_000993"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEG1106879.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            1961..2659
                     /locus_tag="SCL80_000994"
     CDS             1961..2659
                     /locus_tag="SCL80_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEG1106880.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(2827..5466)
                     /locus_tag="SCL80_000995"
     CDS             complement(2827..5466)
                     /locus_tag="SCL80_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1106881.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(6085..7116)
                     /gene="queA"
                     /locus_tag="SCL80_000996"
     CDS             complement(6085..7116)
                     /gene="queA"
                     /locus_tag="SCL80_000996"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEG1106882.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            7309..8484
                     /locus_tag="SCL80_000997"
     CDS             7309..8484
                     /locus_tag="SCL80_000997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1106883.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            8488..9129
                     /locus_tag="SCL80_000998"
     CDS             8488..9129
                     /locus_tag="SCL80_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106884.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            9126..10043
                     /locus_tag="SCL80_000999"
     CDS             9126..10043
                     /locus_tag="SCL80_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1106885.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYSFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            10040..10711
                     /locus_tag="SCL80_001000"
     CDS             10040..10711
                     /locus_tag="SCL80_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106886.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(11252..11740)
                     /locus_tag="SCL80_001001"
     CDS             complement(11252..11740)
                     /locus_tag="SCL80_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEG1106887.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(11941..12207)
                     /locus_tag="SCL80_001002"
     CDS             complement(11941..12207)
                     /locus_tag="SCL80_001002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1106888.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(12207..12470)
                     /locus_tag="SCL80_001003"
     CDS             complement(12207..12470)
                     /locus_tag="SCL80_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1106889.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            12946..13131
                     /locus_tag="SCL80_001004"
     CDS             12946..13131
                     /locus_tag="SCL80_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1106890.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(13273..15096)
                     /locus_tag="SCL80_001005"
     CDS             complement(13273..15096)
                     /locus_tag="SCL80_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEG1106891.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(15694..18399)
                     /locus_tag="SCL80_001006"
     CDS             complement(15694..18399)
                     /locus_tag="SCL80_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEG1106892.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(18705..19652)
                     /locus_tag="SCL80_001007"
     CDS             complement(18705..19652)
                     /locus_tag="SCL80_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEG1106893.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            19842..20111
                     /gene="rpsN"
                     /locus_tag="SCL80_001008"
     CDS             19842..20111
                     /gene="rpsN"
                     /locus_tag="SCL80_001008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1106894.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(20287..20649)
                     /locus_tag="SCL80_001009"
     CDS             complement(20287..20649)
                     /locus_tag="SCL80_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1106895.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(20660..21781)
                     /locus_tag="SCL80_001010"
     CDS             complement(20660..21781)
                     /locus_tag="SCL80_001010"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEG1106896.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(21797..22147)
                     /locus_tag="SCL80_001011"
     CDS             complement(21797..22147)
                     /locus_tag="SCL80_001011"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEG1106897.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(22277..23788)
                     /locus_tag="SCL80_001012"
     CDS             complement(22277..23788)
                     /locus_tag="SCL80_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEG1106898.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(24110..25462)
                     /locus_tag="SCL80_001013"
     CDS             complement(24110..25462)
                     /locus_tag="SCL80_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEG1106899.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYFEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(25615..26691)
                     /locus_tag="SCL80_001014"
     CDS             complement(25615..26691)
                     /locus_tag="SCL80_001014"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEG1106900.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            26887..28632
                     /locus_tag="SCL80_001015"
     CDS             26887..28632
                     /locus_tag="SCL80_001015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEG1106901.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(28912..29349)
                     /locus_tag="SCL80_001016"
     CDS             complement(28912..29349)
                     /locus_tag="SCL80_001016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEG1106902.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(29333..30706)
                     /locus_tag="SCL80_001017"
     CDS             complement(29333..30706)
                     /locus_tag="SCL80_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106903.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(31028..31516)
                     /locus_tag="SCL80_001018"
     CDS             complement(31028..31516)
                     /locus_tag="SCL80_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1106904.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEVFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(31538..32140)
                     /locus_tag="SCL80_001019"
     CDS             complement(31538..32140)
                     /locus_tag="SCL80_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1106905.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT         9200 a         6883 c         5941 g        10159 t
ORIGIN      
        1 acacacccga ttattcaata ttgagcaata tatcccatgc tcttatctct ttcttttttg
       61 aataaaaaat gaggtaggac atctttctgc cctacctcgt ctttaaataa gctttgatac
      121 ttatttttct tccttctctt ctgtctcttc cagcgtttct gttgtttcag tttttacttc
      181 aactgtctca gctggttctt ctgttgtttc aacagtagtc gttgcatcat ttgttacgac
      241 agtccctggt ttgaccgttt tgattgctgc acgatcgtac tcaaggaaga tcccttcgca
      301 gtctaaaaca actgtccgtt tgtcattgtc gatttctgaa acaactccat gtaatccgcc
      361 gatcgttacg actgaatcac ctggtttcat gctatctaac aagttttgac gttcttgctg
      421 ttgtttcttt tgcgaacgtg acataaaaaa ccacattgct aatagcgcga taaacatcac
      481 aagcattggt aatgttccca tttttttcac ctcactaatt ctgtatctac aaaaaacact
      541 ttagcagaaa aatgctgata acactagttt tttacttaat ttttaaaaga ttttccttta
      601 gaagcttttt gcgttttctt tattgaaccc gtattcttca aaaaaggctt ctctaaattc
      661 aagcaaatta tcatccatga ttgcttgacg tacttgtttc attagattca acaagaagta
      721 taaattgtgg taagaagtca aacgaatccc aaatgtttca tcacatttga tcaaatggcg
      781 gatataggca cgtgtatagt ttttacatgt ataacaatca catttctcat caattggacg
      841 gaaatcatgg gcatatttcg catttttcac tactagacgg ccttgagaag tcatacatgt
      901 gccatttcga gcgattcgag taggcaagac acagtcaaac atgtctactc cacggataac
      961 accatcgatc aatgaatcag cagcaccgac acccatcaag taacgtggtt tgttttcggg
     1021 gatcaacggt gtagtgaatt ctaatacacg attcatttct gatttaggtt ccccgacaga
     1081 taacccgccg attgagtaac cagggaaatc catcccgact agatctttcg cgctttgacg
     1141 acgaagatct tcaaaccctg ctccttggat gattccaaat aatccttgac ggtctggatt
     1201 agcatgagct ttcaatcctc gttctgccca tcgagacgta cgttctactg attttttcac
     1261 gtagtcgtaa ctttcatcga atggtggaca ttcgtcaaaa ctcatcatga tatcagagcc
     1321 caatttattt tggatgttga ttgctttttc tggtgataaa aacatacgcg aaccgttcaa
     1381 gtgatttttg aaatgcacgc cttcttcttc gatattccgc atatcactta atgaaaaaac
     1441 ttgaaagccg cctgaatctg ttaaaatcgg ttgatcccag ttcataaatt tatgcagtcc
     1501 gccagcttct tctactaagt cttcaccagg gcgtagccat aaatgatatg tattacttaa
     1561 aataacgcca gcacccattt ctttcaactc ttctggggac attgttttta ctgtagctaa
     1621 tgttcctact ggcatgaaca ttggtgtggg gaaagttccg tgtggcgtga tcaattcccc
     1681 taaacgcgcg ccagtatgtt tttctttctt gattaaacga tagcgaatgg caggttgcgt
     1741 cattcatttc cctactttct atataaaata tcttgacaac tcccaccatc ataatcgttt
     1801 caagtgcttt ttgcaagttc cctcggcttc cttctacttt atttaatatt ctttttttta
     1861 tccttttttt atgaataaca aaaaaaagaa aaacattatg acagaaatat tttttgatac
     1921 atggtatatt ggtgttatta aagaaaagga gggaaaaatc atgcaatctt ctgcagaatt
     1981 aaaacgccaa gcaaaagaga gtctaaaagg acgttgggga caagctgttt tactaaatct
     2041 aatccctaca ttgattggta ttgctactat tcttcttatt atgattccta ttatcttgtt
     2101 tgccaccacc atatatgatg gatcagcgag cgtctcagaa gcgacaactt caggaggcgg
     2161 tagttcagga ggcggtggcg gaattatttc cagcatcatt agcgctttgt tcatgagcgg
     2221 tatttcttgg acttatcttg atattttacg tggacaaaaa gatcagatcg aaccattcaa
     2281 agatgcattt cgtggattca aaggtttgtt tatcggcgga gtgatcagct tagctgtgct
     2341 gatctcgatc tttacaacat tatggggttt gctattagtt attcctggaa tcattaaaag
     2401 ctattcttat tctcaaagct attttattta ctacgatatc gtccaagaaa caggacaaac
     2461 acctaaagta ctggatacga tcactgcaag tcgaaaattg atggatggtt ataaaggaca
     2521 acttttctgg ttagatcttt catttatcgg ctggcatatc ttagcattag caactgctgg
     2581 tattggttat ctatggctca gcccatacat ttctgcaaca aaagcagcat tttatgaaca
     2641 attgccaaaa gatatttaaa agagagtgtg acttgtgacc gagggggtgg caggagaaca
     2701 ccgtttatcg agaggcagta aaagaaacat tcagcccatt agcaaaaagg aggtgtgaca
     2761 acattcatgt cacacctcct ttttgtatag ttccagtaaa aagaaaatca acttagaaac
     2821 agttaatcat tcctcttctt ctgcttccgg taatcgatgg aacttgatca gttcattcac
     2881 cgcgattggt acgatagata aaatccctac caagaaccag tgattcaaac tgatgtgtac
     2941 taggccgaat agttcttgcg taaatggcag caaagcaaca gccgttgtaa tagctaatgc
     3001 cagtaacgcc atttctacca aagctttatt ggtcgctaaa ttgactttga agatcgattg
     3061 gctgctgcgt acattgaaca catgcagaat cgatgaataa gctaagatca agaatgcaac
     3121 agtttgaccg acatgctgac ttgcgtctac ccatgggctg atagtatcga cataactacc
     3181 taaataataa ccaaacaagg ttacgatggt gaagacccct gcatttatgg caattttttg
     3241 caataaacct cgagcaaaga tcccttcatt tttcggaatg ggtggctgat ccatgatgcc
     3301 gtgttccgct ggctctcttc ctaaagcaaa tcctggaagt ccatcagcaa ctacattgac
     3361 aaacagtagt tgcacagctg taaatggtgc tccccatcct aacagcatcg caatcaaaac
     3421 aataaagatc tccgaaatat tacagcttag caaaaagttg atagctttac gaatattctg
     3481 ataaaccgaa cgtccttgac tgacagcgtc tacgatcgtc gcaaagttgt catccgtcaa
     3541 aatcatatct gcggcacttt gcgccacatc tgttcctgtg atccccatgg cacacccaac
     3601 gttacttgct ttcaacgctg gtgcatcatt cacaccatcc ccagtcatcg ctacgactgc
     3661 acccgtacgt tgccaagatt tgacgatccg gatcttgtct tctggcgtta cccgagcata
     3721 gacagagaga gctttgacac gcgcatctaa ttcttcgtct gataattgat gcagttctga
     3781 tcctgaaagt gcttctgacg gatcattcaa gatacctaat tctttcgcta tggcactggc
     3841 agtcaccaca tgatctccag tgatcatgac tgttttgatg ccagcctttt tcgctcgctt
     3901 gattgctcct ttactttctg gacgtggtgg atcgatcatt ccgatcagac caatcaaacg
     3961 taagtctttt tccagtgctt cagaagaaat atcttgcggt tcttcgtcat aagtagcata
     4021 accaacagca atcactctca gtgcccgttt accaaagcga tcattaacga tcgccgcttg
     4081 ttctacatcg cctgaactaa agcgcggagc aagaacatcg aatgctcctt tggtgattga
     4141 gatgtatttt tcgcccattt ggtgaactgt tgtcatcatt tttcgttcag aatcaaaagg
     4201 aagctcgttt actcgtggat atttttcttc taattctgcc tttgtatgat aattctcttc
     4261 aacagccctt acgatggcta cttctgttgg attacctttg ataacaaggt cgtcatcttc
     4321 ttgctcgacc gtcacatctg tacatagtgc agccattttc aatatttcca tcgcgctgtt
     4381 cgtcattgaa tcttcgatat tcgttacttc atcttctttg ctccataccc ggcggacgcg
     4441 catttcattt tgtgtcagcg ttcctgtttt gtctgagcag ataacattag cggttcctag
     4501 cgtttcaact gctggcaact gtcggatgat cgcatgtttc ctcgccatct tctgcacacc
     4561 gtatgcaagt gtcaacgtaa caataacagt caaggtttcc ggtacagctg caacagccaa
     4621 ggaaatcgaa gtcatgaaca tctcaaggag tggttctcct tgcagttcac caatcacaaa
     4681 tactaaagcc gcagccgcta aagcgatcat actgattcgt ttgcctagct gattcaatcg
     4741 gacctgtaaa ggtgtttttt gcatagtatt ttcgttcaaa agatccgcga tcttacccat
     4801 ttctgtagcc atgccaatgg cagtgacgat cgctttccca cgacctgcag caaccgtaca
     4861 tcctttaaat accatattaa gttggtcacc taatgactca tcttcttcag caataaatgc
     4921 tgcatctttt tcaactgctt cactttctcc agtcaacgcc gattcttcta cacgtaattg
     4981 tgatgcttcg atcaaacggg catcggcaga aatagcatca ccggactcta aaacaagaat
     5041 atcacccact accagttgct ctgactcaac agtggtcact ttgccgtctc gtatgacagt
     5101 tgccgtttgt ttattcatat cttgcaagga ttccagtgct ttttctgcgt ttccttcttg
     5161 gacaatcgcc aataccgagt tgatcacaac gatagcgatg atcagcagtc cttcaaataa
     5221 atccccatgt tctgtagcaa atgcggtata aaaggaaatc accgctgcta caagaaggat
     5281 gatcgttgta aaatcggaca agcttcgaaa gaattttttg atcatcgatt ctttcggcgc
     5341 ctcatcgaat ttattcaatc cctgcttttt tcgccgttca tccacttctt gtgaagacaa
     5401 cccagtatct ggatctgctt ctgttacctt ctttacttct tctattgtct gcttgtattc
     5461 ttccatccta ttcctccttt aaaatcttcg tacaaaaaaa ggtacttatt ttactataac
     5521 atcctttttt tatttacttc aaataaaggg cttatcgatt atttgttaag aaagcgtaaa
     5581 atttaaagtg cttatatttg caaacgctac caaaggatgc tattatatag atgtaaggaa
     5641 ggaggatacc atcaaataga cagaagaatt tctcgataga acagtgtaag caaacaaagt
     5701 tgttagaagt acaggtttca cccagacatg cgtgtagagt acaaacctat aaatagtgaa
     5761 tgcgtatgga tgtttggatc acgaagtatc cgaaacaaga agtaatgcga tacgtaacaa
     5821 ttgaataacg taaacgtctg aaaattgaat aggaacaaga gatgtttcaa atgttgaaga
     5881 tgtaacgaaa acaaaatttt tgtttaacat gaagaaattc aacaccgtgc tttgcatgta
     5941 tgatggaaag ttacaatcat ttctgtttat ttgaatcctt ccgaggtaca agaaagccga
     6001 gcttatcgag agctcggctt tttctttgtc ataaaatctt tgtaaagctt ttttgttatt
     6061 caaaactagc ggcttttcat aaccttattt cacgaacatc gcatctccaa aactaaagaa
     6121 acgatagcgc tcttcaatcg catgctggta agcagataaa gtcaattctc ttcctgcaaa
     6181 ggcactaact aacatgacta aagtcgattt tggcaaatgg aaattagttg agaaagcttc
     6241 taccactttg aattcatagc caggcgtaat gaaaatatca gtccatccgc tgtctgcttt
     6301 gatttctcca ttaaatttcg ttccaatcgt ttctaatgtt cgaattgaag ttgttccgac
     6361 agccacgatt ttcccgccat ttttacgtac ttggttcaat cgctcagccg cttcttccgt
     6421 caaacggtag aattcactat gcatatggtg ttcttcaatt ttatctacgc tgacaggtcg
     6481 gaatgtacct aaccctacat gtagtgtcag atagactaat tcaacaccct ttgcttcgat
     6541 ctcatcaagt aattctttcg taaaatgcaa tccagctgtt ggcgctgcag cagaaccatt
     6601 ttctttcgca tagacagttt gatagcgatc tgggtcttcc agacgttctt tgatatatgg
     6661 aggcaatggc atttctccca atgattcaag aatctctaga aaaattcctt catactcaaa
     6721 gtcaatgatc cgtccgccgt gttccaattc ttctttcact actgcttgca atctgccatc
     6781 accaaaagag attcttgtcc caactttggc acgtttcgca ggtttgatca atgtctccca
     6841 cgtatctcct tgtgtattcg ttagaagaag gacttctaaa tgcgcaccag tatctggttt
     6901 ttctccatat aaacgagcag gcagaactcg tgtattattc ataactagcg catcaccagg
     6961 ctgtaactct tcaatgattt catggaaatg tttatcttcc atctctccag ttttccgatc
     7021 caaaacaagt aaacgagaat gatcgcggtc ttttaatggt gtttgtgcga ttaattcttc
     7081 cggcaagtca aaatcaaaat cttctgttgt taacatatcg tcactcactt tctatcatgt
     7141 gcttcatcat tctatcatgt ttttcgttct gttggcaaaa tcagcacttt ttcacttcta
     7201 ttttttgttt tcctaaataa aaaaactacc ctatttgtat tttttcattg ttttaggcat
     7261 actagtaata gatagttctt cattgaaaat agaaaaagag gtggcaccat gattgaattt
     7321 caaaatgtgt caaaaattta taaaggtgga aaagtcgctg tagaggacgt caatctttca
     7381 tttgaaaagg gtgagttcat ttgttttatc ggtacgagcg gaagcggaaa aacgacgacg
     7441 atgcggatga tcaaccgaat gactgatcca acacaaggga aaattttgat tgatggaaaa
     7501 tccatccaag aaattgatcc agtggaattg cgacgtcaga tcggctatgt tatccaaagt
     7561 attggattga tgcctcacat gacgatccgt gaaaatatca ctcttgtcca aaaattgttg
     7621 aaagtcagtc aagaagaacg gaataaaaca gctgaaaaaa tgattgatct tgtggaattg
     7681 ccccgtgaga tgttagatcg ctacccacat gaattatctg gtggtcagca gcagcgtatt
     7741 ggagtcgttc gtgcccttgc agcgaatcaa gatatcatct tgatggacga gccattcggt
     7801 gcgcttgatc caatcactcg tgattccttg caagaccttg tcaaagattt acaagagcgt
     7861 ttgggaaaaa cgatcgtatt tgtaacccat gacatggatg aagcgattaa actagcaagc
     7921 cgtatcgcca tcatgagtga aggccgtgtg atccaatttg atacaccaga aaatatttta
     7981 cgtcatcctg caaacgattt cgtagaagag ttgattggag aagatcgcct actacaagcc
     8041 aagccaaatg cgacaagagt cggcgaagtt atgctaaata cagcaatcac gattacaccg
     8101 gaaaaatcat tacaagaagc aatcaaactg atgagggaaa aacgtgtaga tacgttgtta
     8161 gttaccgata attcgaatgt attaaaagga tttatcgatg tagaaacgtt gaataaaaaa
     8221 cgaggaaaag tatccagtgt cggcgacatt ttgaataaac atgtttttta cgtaaaagaa
     8281 tccgcctatc ttcgtgatac cttgcaacgc attttaaaac gcgggctgaa atacgtgccg
     8341 gtcgttgatg aacagaacaa agtcgtaggt atcctgactc gtgcatcatt agtggatatc
     8401 gtctacgatg tgatttgggg agaagatccc gcaactactg atgttgcaga gtcagctact
     8461 cctgaagaat ctaaggaggt ctgacctatg cagcagtttt ttcatcagca cggttcagaa
     8521 attctctcaa aaggttttga acatctttat atttcaggac ttgctttgat cattggtatc
     8581 atcattgctg taccgcttgg tgtcttactg acacgttttc caaaagctgc cacgattgtg
     8641 attggcttaa ccagtgcctt gcagacaatc ccttctcttg ctttattggc attgatgatc
     8701 ccgctatttg gcgtaggtaa attacctgcg attatcgcac tctttattta ttcattattg
     8761 ccgattttac gtaatacgta cattgggatg aaaaatgtcg attggaatta tcgggatgtg
     8821 gcaaaaggca tggggatgac tgagttccaa tccatttttt ccgttgaatt gcccattgct
     8881 atgccaacga tcatggctgg tattcgttta gctgccgttt acgtgattgc atgggctaca
     8941 cttgcctctt atatcggcgc tggtggttta ggcgatttga tttttagtgg actgaataat
     9001 taccaaccag atctcatttt ggcaggtacg atccctgtaa caattttagc actattggct
     9061 gattggcttc taggtctctt ggaacaccgg ctgacaccga tcgcacttag agaggagaat
     9121 gaagaatgaa acgtatacta aaaatgacca tggttctttt ctctcttctt atacttgcga
     9181 gttgttcctt ccctggtctt gccagcaata cagatgaaga caccatttcg atcacaggtg
     9241 gaatcacttc agaagcacag atcttagcaa gtctagttgc cggaatggtt gaacattata
     9301 cagataaaaa taccgcaatc atcaacaatc tggctactac aactatcaac catcaagcga
     9361 tgatgaacgg agatgcttca atttcagcgg ctcgctacac aggaacagat ttaacgacta
     9421 cattgaacct tcctcctgaa aaagatccga aaaaagcgtt tgctaccgtg aaagatgaat
     9481 ttgagaaaag atacggtcaa acgtggttcc cgtcctatag ttttgaaaat acgtatgtct
     9541 ttcttgttcg aaaagacacc gcacaaaaat accatctatc taaagtcagc gatctgaaaa
     9601 atgtagcgga tgaattagta gctggtgtag atacttcttg gatcaatcgt aaaggagacg
     9661 gttatgacgg cttccaagaa acttatggct tctccttcaa ttcgatatta cctatgcaga
     9721 ttggacttgt ttatgacgca gtcgaagcag gaaaaatgga tattgttctt ggctactcta
     9781 cggatggacg gatcgccagt tatgatctag tcatgttgga agatgatcgt caattcttcc
     9841 caccatatga tgctgctcca gttgtagaca acaagatatt agctgatacg cctcatttaa
     9901 aagaagcctt aaataagtta ggtcatacca tcagtacgga aaaaatgcag gaattgaact
     9961 acgaagcaga taataatcta gtggaaccat cagtagtagc acaacgtttc ttacaagaaa
    10021 accactattt tgaagggaag tgaagcaatt ggataaaatg aatgtctttc aacaattttt
    10081 ctattatttc caggaaaatg gctcctatat ctttgcacaa tttatccgtc acttcctgat
    10141 ttctatctat ggtgtcctct ttgcagcagt tgtcgggatt ccggttggga tcatgatttc
    10201 taggagacgt aaattagcaa actgggtgat tcgtttggcg aatatcatcc agacgatccc
    10261 ttctctagcc atgatctcca ttttaatcat tggattaggc ctgggagtca atgtcgtgat
    10321 tgttactgta tttttgtatt ccctcttacc gatcatcaag aatacgtata ccgggatgat
    10381 ccaagtagac aaaaacatcc ttgatgtcgg caaaggcatg gggatgactg ctagacaacg
    10441 actattcatg gtagagttac cactctctgt ttctgttatc atggcaggca taagaaacgc
    10501 gttagttgtt gccatcggga taaccgcgat cggtgcattc gtcggtgctg gtggtcttgg
    10561 agatatcatt atccgaggaa ccaatgcaac agagggcaca tcgattattt tagcaggtgc
    10621 attgccaact gcattaatgg cgatcatcac ggactggctt ttaggtattt tggaaagacg
    10681 gctagatcca gccagccgta catcacgctg atgttattgt aaaaaacaaa agcttctgcg
    10741 accatttgtt ggtcgcaaag gcttttttag ttctgtcatt tttaaggcaa taatccactc
    10801 tgattcttcc ctccttatgg gtaaagagtt gcttttctta gctatttatc cattccgtat
    10861 actccaactt catactgcca acgggttcat atctgcatcc accgactaag tttttttctt
    10921 atccgctaag gcattttgcc aatccatttt cccatcactc gtcatggaat tcataccaca
    10981 tgatctctaa atactcgtct gcttgttctt tgaattgggc atcaattctt acatcctttg
    11041 gcgtggcttt catttaagcc ttttccatca gtcctcgatt gttcgatgcc ttcagctgca
    11101 tcatcaaaac tggtgactgc cgtatcggta acttcgatca tcttttatac aatcgggaac
    11161 ttgccatcct catccttggc taaaaacata tctagccatt ccagcttctc gccatgggag
    11221 gtttgcggtt cctaaaaccg gtacacgatt cttatggact gcatccgtcg caactagact
    11281 tggcgatacg atgatccttc accagaagag cacccccgta aaccaattga tcgatatatt
    11341 gccaataaga aaagacattg gcgtaaaaga tatcgatccc tcgtgatgca ttgccgttga
    11401 tactgctatt catggccgaa agcgccacga ctttaacttc ttccgtttga gtgacattca
    11461 tgttcggcaa tgtcgctttg gatacccgct ctgctaaagg aacgacactg gtattgtatt
    11521 tcgtatctgt atcttttcca aaagaccatg ccaacgaatc ctctgggaac caataagacg
    11581 actccggttg attgtccatt gcttttttca acatactttt ggctgtttct cgataaacga
    11641 catttccaat tgtcttcgcc tcactttggc gataatcgat ggtcgttgcc actgccaaca
    11701 ctaacaacga tgctccaatt ttactgattt tccttgtcac acttcttcct ccttgtttta
    11761 cataaacccg tcaacattcc attcctaaac tcaaacaatg acccttcaaa ggtggagata
    11821 taggaacaaa ttaaagaaat catgcaataa atccaagaag gttggtttta gaacaaaaaa
    11881 agagtctcct ttttggggag actctttgtt aactatctaa aaaggttggc tcttctcact
    11941 tcactcataa tgtgtccagg cagaataaat ttctacaact ttattttcct catcaacttt
    12001 gtaaaccact cgatgctgtc tattcagcct gcgtgagtat ctgccttcat gtgtaggtct
    12061 cagcttttca aaagactgcg tgggcatata tggatcttcc ttcaatgtct gaatcacttc
    12121 ttcaaattgt ttctttaaat tcgtttgctt gatttttctt aaatccactt tcgcagagtt
    12181 tttgattgcg actgtatagt tactcatcaa agagcttccc aatcaatatc atcgatattc
    12241 gtaaagctac tgtcgtcttt ttcgcggtct cgtactttat ctaaagtacc tgtttgctca
    12301 agaaaaaggg tttcttgaat cgctctccag tcttccagcc caatcaatac agcattgttt
    12361 ccactacgat cactaataat ctcgatttca gtatgatttt catttacctc tttcaataac
    12421 tgataaaaat ttttacgagc agtgctagga ttcatcgttg tattagccat tgtctctcct
    12481 cctttttctg atataattat agcgtacttg attaagtacg tcaaaaaaat acaataaagc
    12541 acttaaattc agcaaacttt tcctcttttt tacatagaga aaaagacccc tttttaggga
    12601 ggatctttgt ccacgctcac ttcttactcc tttacgacta gcggtagcca aacgtaaagt
    12661 aattggtaac aacgtatcat ctttagcaaa ggtcgaatcc aaagcctgtg agtctatgca
    12721 acaacaccaa tacacgaagg ttcctcgttg cagtgtgact tgattgatat cttgaataac
    12781 ctgaggatac gcacgaaaga tttgtaacgg ttattcaaaa ctgattttgg ccgtcaagtc
    12841 ttctactgcc tcaaaaatga ttgcctcttt cgtctcctgt cattcatgtt cactatcgat
    12901 ttgatacttg cttagaccgt tgggtttgtg aatcaccaat taaaaatggc actgagttat
    12961 ggtaacaaaa aatggtcact tgacgaggct gagattccta cttatgaaga cacatggcac
    13021 ttaagagtcg aggtaaagga agaagctgaa aatataggag gctgtacatt atttggcaaa
    13081 ggattcggaa attatgatca agacatcaaa atcaaaatgt tttacagcta aataggaaga
    13141 ataataacat ttcatacgct gttcccatct ttaatggctt atagagggca cctttagctg
    13201 agaataagac aaaacagacc ctctagcttc aattaagagc tagagggtct gtttttgatt
    13261 ttaaaacttg gtttatttct tcaaatggta gctataggtg ctgacaacga tattttcacg
    13321 ccaagacaat cgcaatctta accgcaaact tgtttggtta tggagaatat tctgccagcg
    13381 tctgtttggt acaaattgag gaatcaacac agtagttgta tagttttgtt tcgctgcatt
    13441 tttacttacg atatctacat aacggataat tggattttca atcgagcgat aagaagagtg
    13501 gacgattgaa aaacggacat ctgggaaatg ctttttaaat tctgcttcga tttctttttc
    13561 tttttcgaca tcttcgtcta gcgaaacatg catcgcaacg acataatcac cgatcgaacg
    13621 tgcgtaattc aacgcaccga tatttacccg tgtcacattc ccgactaata caagaacggt
    13681 attcccgtca taatcatgta gttttgcatc attttctaaa cgaagctgtt ctgctacttt
    13741 tttataatga tcatggatct tataaaaaat gaacataacg attggcataa tgatgaagaa
    13801 aggccaaatg tcccctaacc gatagacaaa caaaatcgca atgattgcat atgagatgaa
    13861 cgcacccgta atattggcaa tcgatttgct taaccatctt tttccttctt ttttccattt
    13921 gacgaccatc cctgtttgag ataaagcaaa tggaatgaac acgccgatag agtataacgg
    13981 aatcaagcgt tctgttgagc catggaaaat aaacagcaat acgatcgaac ctagcgctag
    14041 tgtgataatc ccattggaat agcctagtcg atccccacga tcttgataca tgtgaggcat
    14101 gaatttatct tttgccagat tgtatgcaag aactggaaaa gcagaaaaac cggtgttagc
    14161 agcaactgct aaaatcagcg ctgtagcaaa ttgaaggacg taatacaaaa taccatgtcc
    14221 aaagacagct ttaccaattt gagacaaaac agtgacttct ttttcaggaa cgatcccata
    14281 ccaatagttg ataaatgtga tccccacgaa gaagaaacct aagatcaatg ccatcatcgt
    14341 caatgtagca gcggcatttt ttgctcttgg cttcttaaag aatggtacag cattactaat
    14401 cgcctccacc ccagttaaag aggaagaacc agaagaaaat gctcgtaaaa tcaaagcgat
    14461 agaaacaccc ggcacagccg ctcctggaag tgcagtagct tgcaatggca cagcacctgt
    14521 cacgatatta tataaaccaa caacgatcag taacgagatc acaaggataa atgtgtacac
    14581 cggaaataat aggaacgatg cagattccct cagcccacga agattcagca tcatcagtag
    14641 caaaacgatt gtcacagaaa tagcgacttg atgaccgtag agtgctggca ccgctgatgt
    14701 gatcgcttca gcacctgctg agacagatac tgctactgtc aacatgtagt cgatcaataa
    14761 agagccaccg gaaatcaagc cagcattctt tccaagattc tcactgctga cgacatatgc
    14821 tcccccgcca tgaggataag catggatgat ttgacgatat gatagcgtta atgaaatcaa
    14881 taaaataata acaaaagcgg caatgggtag cgaataccag atggctgcag cagacaaagc
    14941 gactaaaacg acaacgattt gttccgtacc atatgcaata gatgataacg catcagacga
    15001 caataatgcc aaagcagcaa atcgggttaa tttgtgctca tcattttcag cagattttaa
    15061 aggcttacct actaacaacc tttttaagta atccaatttc taaactcctt tacacacaat
    15121 tttacttcaa gttttatagt cgaaaattta tagtaaacaa gatgttatag tacgctcact
    15181 gaccttaaaa gtcaataaac tttaagaaaa taattggaaa aaaacagaaa aatatcaagc
    15241 gctttttcaa acgctttaat acagcaaaaa tctaacattt ttttatgggc tttttctttg
    15301 aattctataa taaaaggata gaacttgact tagaaaatca agttctatcc tttttgatat
    15361 cttaactttt ggaagagaga tgtaaggctg ctttctaccc atcattctta ctactcgaag
    15421 catagcgcag ttgtcacaac ctcttgataa acggtgttta aaagcttgca tctgcggtaa
    15481 taagcccaac caaattaaaa acataagaag ctgtttttat ttggtcgttc ttattacttg
    15541 atgcagagca gcttttttac aacctcttgg tacacggtgt tcaatcagct gaccctcggt
    15601 attagctcaa aaaaacggca aaatatgagg aactattttt ccattttttt ctctaatact
    15661 caggtcaaaa cactgatttc acaacctctt tttttaatga aaaatatggc tctcatgcgc
    15721 cgcttctttg aatttcgtca ttttttcttc gatgaacgga tagattgccg ttcctacgat
    15781 tccaaagaca acaaacaaac cgatcatgat ccagattgct ttcgtagcat ttggccaata
    15841 aattcctcct gcagattccc gtaacaagtt taccgcatga gtaaatggca agaacggatt
    15901 gatcatttgg aagaatttcc ctgagacttg gatcggatag ttcccgcctc cgccagagat
    15961 cgacagtact aagatgataa tagcgatccc tttaccgaca ttatcgaata atgcagccaa
    16021 gacatagacg atcatcatga acgctaaggc gatcaagaca gcaaatgaca cgctgtagac
    16081 cggattttgg acatctactc ctagtaagaa aatattccct aatgtaacga tcaagctttg
    16141 cgcaatcgcc attgtcaaaa atgtcagcat acgagcaacg aattgttctc gtttcgtaaa
    16201 attgtttcgg tctttctttc ctaaatagta ttctgtcgta gcaacacttg aaagaagaac
    16261 agcgccgacc cacagacata gtgcagtata aaacggggtg ctagctgaac cgttatttgc
    16321 gattggataa agcgcattgg tttgtagctc aacaggttgt gtaaagaagt cactttcttt
    16381 tgttgcgtct agtttcaata atttgatgac ttcgcctaga tcgacttctt gttcgccttt
    16441 tcggatcgcg tctgctgctt tttggatacc agctttgata tttggccagt cattgttgat
    16501 caagtccgca gcagcgttca cagctgtttc gacttctggc attttttcat ttactgttgt
    16561 caatgtaccg gtgatttcat tttttagtgc tggccaatca ttttggataa aatcagcggc
    16621 caatcctaat ttttgctgaa tgatcggtaa ttcattgttg tataggtctg ccccttcatt
    16681 gatgccattg acgatggtat ccatatttcc attcagcatt gtattggcat catggatttc
    16741 ttgtccaatt gcaggcattt ctttttgata tttttctaaa attgccactg cattcgttac
    16801 agttgctttc gttgagctga gaagcgaatc aaagtcgatc tggtccgctt tgctcagtgc
    16861 gccttgtgca gtcccaatcg tatcgatgac ttggtctagt agagaagtga cagtggaact
    16921 gatttgacct gtattgatac cagcaatgat gccattgata ttattaacaa cggcatcaat
    16981 ttgatctagc aattcatcaa tagccttcgt gtcttcattt tgaacagctg tgttcaaagc
    17041 agataggcgg ttttgcagat tgcttagcaa gggttttaat tgatttagct gatcaataat
    17101 tggctgtaat ttcgtatttc cagcacttgt ttgcaattta gtcaggaact cgaccagttt
    17161 atcgattgca ttttgttgtt gagatattcc gtcaataaag ccgttcaata cattgctgat
    17221 gtttttcttt tcatcatcag tcagcttacc atcttcaata gctgttctta cagtatctat
    17281 taacgaacct gtcgttgttg ccacttgtcc aattgacttc aatgtaacat ctacactgtt
    17341 tgtgatgctt ggtatcgcat ctttcagctg agaggcacct gattttgttt ggtttgctag
    17401 atcacttgcc tgattttcta aatttttcac atctggcaag atatcttgta cttgttggat
    17461 gatcgtcaag ccttgttttg cttcatcgat tccttggttc attgtctctt ggatcgaagc
    17521 aaaatcgccg tcgatctctg ctagttgctt accggcattt tggatctctg ggattttttc
    17581 ttgcaaatca aggatgattt ttgcttgatc tttcaactca ggcattttgt cattcaatgc
    17641 aacgactttt tcacccatct catctacttt agggatgtaa tccacgaatt catttgcttt
    17701 attcagtttt tccttgattt caggtagttg actttgtaat tcgaggactt gttttgtgta
    17761 gccgtcgatc gtgtccaaat tttcatctgt tgataaaatc atatctttaa ctttattgat
    17821 acttactagg ttcgtatcga tgtcgtaacc tatctcattg aatactttca ataaagtcga
    17881 actggctgtt ttgataaatt catttgtgat ttgcgattgg atcgaacttg ctcctttatc
    17941 ggtgattttc ggtgcaatcg cattgatttt ttcatttacc gtatattcga tttttggttt
    18001 cttgatatca ccactagtaa aactcagcag atcttctgag aaatcctttg gcaagtaaat
    18061 acctgcgtaa tactttccgg aacggactcc gtcttctaat tgttcttttg aatcaacgaa
    18121 ttgccatcct aattgtttat ttttatgaag cgattcgatg acttgctccc caatggccac
    18181 ttctttacct tgaaactctg ctggcttgtc tgcactgtaa acagcaattg gcagctctcc
    18241 agtatttcca tatggatccc ataatgcttt gatgttaaac cacgcatata gcgaagggat
    18301 gatcatgatc gcgatgatca gaagtgttgc aatcgggttt ttgaaaatcc gtttccagtc
    18361 aagcaaatat aatcgaagcg tatttttgat tgactgcata gttctccccc ttcttctttt
    18421 ttcagctgaa ttactttacc atgtatttca tacttttttc aatcggcttt tttcaaaaaa
    18481 gtcgaatttt tggacatatc acggcaattg tctcattgta atctcttaca tattgagaaa
    18541 taattaagga ttacagcctt tactttatca tgaaataaaa gggaaagata attttatgcc
    18601 aaagttttta tctattatac gtatacgcaa aaaagctaaa acattttttg tctttgcccc
    18661 tacaaaaatg tttcagcttt tttcgttttt tattcctgta tttcttattt ttcgaatgac
    18721 aggatgattt tcccagcagg aggatgtcct tcaactagct gatgtgcttg gatgacatgc
    18781 ttaagtgaag cagacagatt cattgaaata aaaacatgaa aggctccttt ttggtaggct
    18841 ttgattatgc cagcaaaggc ttctgcatct agatattcct ttcttggaac aaacgattca
    18901 taaaagcctt cctttttttg ccgcaaatct agatctggga ggtcatttaa tgcaacatac
    18961 ctgccgcctg gtttcatgat ctggatgccg gtttctcctt tgatacttcc tttcgtcgca
    19021 tcgattacaa ggtctgcctg atcagcaaat tgcagacctg gatttgtttt atcataagca
    19081 gcaaatgctg aagctcctaa tttttttacc ttttcctcat ttttactgga tgcactggtt
    19141 aagattcgga ttcccttttc atggagaagc tggatcagac ttgacccgac cgcacctgat
    19201 gctcctagta tcattacagt atctgtcggc tggatttccg tcaaatggtt gattagatta
    19261 taagccgtaa ttcctggcgt gaccattccg gctgcttctt cccaactcat tttatctgga
    19321 atttttgcta cttttgctga tggcaacacg acttcttctc cgtaagtccc gccgactgca
    19381 tggacagcta ctcgatcacc tacatgaaca gttgtgacat ctgaaccaat ctctgtcaca
    19441 ataccagcgc catcattacc tggtacataa gggaatttca atgttcgtat ctctttcatt
    19501 tctcctaaac gcaaggcaac atcataagga ttcacactga atgcttttat ttctacacga
    19561 acatgtccag aaccgacttc tctgggatga gcatcgattt cttcaaaaac atcttgtgct
    19621 tctccgtaac cgttaattgc aaatcttcgc atggtcacca tctccctttt tctttaagtt
    19681 tacgtttatg cctttatttt gacaaaaaaa atgtcttttc gttttggcgc atgattcaaa
    19741 acaaaatagt tgtctttttt cgcgtaacgt gttaaattat ttatctaaaa gcgtaaagat
    19801 tacgatttac gctaataaaa aataattgga ggaatttgtt gatggctaaa aaatcaaaga
    19861 ttgccaaagc aaaaaaacag caagaaacga ttgaacgcta tgctgcttta cgccaggaat
    19921 taaaagcgca aaaagattac gaaggcttgc gtaaacttcc tttagatgct cgtccagaac
    19981 gattgaaaaa tcgtgattta atcgatggac gccctcgcgg atacatgcgt aaattcggga
    20041 tgtcccgtat aaaattccgt gaattagcac ataaaggttt gattccagga gtaaaaaaag
    20101 ccagctggta atccaagtct ggctgtaatg taaaggagga gggcgcatgg accactcctt
    20161 ggatcatcaa aaaagatacg ttcgcttctt ctagcagaaa tgctggtact agaagcagac
    20221 gtatcttttt ttacgcacag ctggtccttt tttatatgta aaggctgcat ggttacttcg
    20281 ctatatttat ttagtaacag gagtcagtgc ttcgataccc acactaattt tcaatgctcg
    20341 gtcaacttct gccataatgg gtcgatccaa atggcagacc ttttctttca atcttgattt
    20401 atcgatcgtt ctgatctgct ccaacaggat aacggagtct ctttctattc ctgttccagt
    20461 agctttgatc ccaatgtgag tcggaagctt aggtttagcc atttttgccg ttatggccgc
    20521 aacaatgatc gtcgggctaa aatggttacc tagattattt tgaataacca aaactgggcg
    20581 tgttccgcct tgctccgatc ccacgactgg agaaagatca gcaaaataaa tatctcctct
    20641 ttttaccata ggatcctcct tattcttgat attctcttgg tatccgctgt cctaatccgc
    20701 aagccacttc atagtggatc gtacctaatt gatctgctat atcctgcata gtgatttctt
    20761 tgtctgcatt tttgccgata agcgtcactt ttgttccgac aggaaattct tgaggcaatc
    20821 ggatcatcag ttgatccata catacacgcc caatcgtttc acagtagttc ccttctacca
    20881 atagagaaaa accctgcatc tttcgtggcc agccatctgc ataaccgatc ggtatcgttc
    20941 cgatccactc tgcttcagga gtgatatatg tttctccata gccgattcct tcacctgctg
    21001 ggagtttttt gacttgaatc aattcagaaa ccagctcaag tgctggctgc aatggataaa
    21061 cttccggtag tgcgtgccca gagggattaa gtccgtacat tgctactcca taacgaatca
    21121 tattgccgat tgtttcatca tgccataaag ccgttgcact attgctgaca tgtacatatc
    21181 gaggaagttc gggaagtttt ttcagcactt ctataaagcg ttctttttgc agattccaat
    21241 atgtatcatc tgcctgatct gctgtagaaa aatgggtaaa taaaccttcc cattctaata
    21301 cacgactttc tttgatgaat gcagctgcct cttttacttc ttcaggtgta caaaatccga
    21361 tacgacccat cccagtgtct gcttttagat gaatcgaaag aggcgtctct agcttacttt
    21421 tctctaaaac agcagctgct gcttgcaacc attcccgtgt acctgcagtt actgataaat
    21481 catgagcaac agctacagtc agtgcgtcgt aaggaaccat attcaagata aggatgggct
    21541 gagtaaatcc agcttctctc aactcgactc cttcatctaa atttgaaaca caaaagcccg
    21601 aagctccacc tgcaactgct gcctcagctg tttcaatagc tccatgaccg tatccgttcg
    21661 cttttactac tgcaaacaat tcttttcctt gcggaagtct tgcaacttca tttgctacat
    21721 tttcagttat agctttttta tgtatcactg ctttcgttgg gcggtgccaa gcgacaacca
    21781 tcgtttttcc ttctttctag tcttccaaaa taatttgtgc aaaagctgtt tcatttgtat
    21841 gagtaatact gacccataca ttgccttcat gaggtgaacg cgtcacaaca ggctgaccat
    21901 tttcattttt taaaatctcg acatcttgaa aggtaacttt tccgatccca gttccccaag
    21961 ctttagagaa agcctctttg caagcgtaac gcccgcctaa aaattctact tggcgatgaa
    22021 aaggcaagga ctggaatagt ttcatttcat cagaggtcaa gattcgtgca atgaattttg
    22081 gtttttcttc gatcagccga gtgatcctag gaagttcgac tgcatcgatc ccaattcctt
    22141 ttatcatatg cttttctctt ttcaataaat ttagaacact ctcattctac caaattataa
    22201 ccattttgcc acaaataagt ccacaaataa caaaaatgaa agtttctctt aaaaaagaga
    22261 aactttcatt ttatgtttaa tcgttatttg aacggattac aaatccgcgt tttttatcat
    22321 tgtgacgttt accgccatct ttttggctgt tattattttt gctataaccg ccttttgatt
    22381 tgtttccacg ataattaccg ccgtcacgac gattacggtt gtttccgcca ccacgtttgc
    22441 catttttatt gtatcctttt ttctgcattg gcaatgggcg ttctggtgtg atcttcactg
    22501 gcactgcatc agctggatct tttgctgtcg tttttagcaa aagtgcaact aaatcttgtg
    22561 cagaatattc ttctaataat ttatctgctg tttgcaggta tttatctaga ccattttctg
    22621 caagttttgt ttcgatttgt tcaactgcag cacctaattg acctttgaat gcttcttttt
    22681 cggttggtgg acgtaaagtc gtcatccgtt ttttcgtcaa gttttcaatc acatgcaagt
    22741 agtccatttc atttggtgta acgaaagtca cagacatacc gccttttccg gcacggcctg
    22801 tacgtccgat acggtgaacg tagctttctg gatcttgagg gatatcatag ttgtacacat
    22861 gagtgacacc tgaaatatct aatccacgtg cagcaacgtc tgtcgctaca aggatatcta
    22921 aatgaccgct cttaaatgaa cgcaatacgc tcattcgttt ttgttgagac aaatcgccat
    22981 ggattccttc tgcacggtaa ccgcgtgctt ctaatccgcg tgccaattcg tctacacggc
    23041 gttttgtacg tccgaagacg attgttaatt caggtgtttg aacatcaaac aagcgagtca
    23101 tgatatcgaa tttttcgtat tcttttgcgc ggacatagta ttgatcgatc aagtcagctg
    23161 tcatttcttt cgctttgatc ttcacatgat gtggattttt catgaattta acgccaatat
    23221 tcttgatagc tggcggcatc gttgctgaga aaagtaatgt ttgtctttga tcaggaactt
    23281 gtgaaatgat tttttcgata tcttccaaga agcccatatt caacatttca tctgcttcat
    23341 ccaaaactaa tgtttgaacg gttccaagtt tcaatgtgtg gcggttaatg tggtcaagca
    23401 tacgtcctgg tgtacccaca acgatatgtg gacgatcttt caagccacgg atctgacggc
    23461 caatatctgc tccgccgtaa acggcttgta cacgtacttt tttgtcgcgc cctaaacgat
    23521 ataattcttc ttgtgtttgg atagctaatt cacgtgttgg tgcgataact aaaccttgta
    23581 attcgtgacg atctggatcg attttttcta acattggtaa tccaaatgca gctgttttcc
    23641 ctgtacctgt ctgtgcttgt ccaataacgt cttttccagc taatgctagt ggaatcgttt
    23701 ctgcttggat aggcgttgct tcttcaaaac cagcgcgttc gactgatttc aacaattctg
    23761 gtgataattc tagttcttta aatttcaaat gaatcctcct atatggttgt taactgagga
    23821 tgtagctttt ttgctacccc gtcgatttgt tcggtcaaag tgtattgttt ctttttgaca
    23881 gtggccacgc acgtttttat ccattcttag cccctgtggc accaccgaaa aatcactttg
    23941 cgaagtttag gcattagaaa agccaaacgg gtcgcgttca gctttatcat tattatgaca
    24001 tctggcttca acaagttgtt tctatggcaa aaccagtcca atttctcttc atctttcggc
    24061 aataggttat tctaacatag tttttcgttt tctgcaagtt tacagatttt tatttcattt
    24121 gctgaagttg ttctaccact tcaaataagc ccatgctgtt gctgcctttt agcataatac
    24181 tatctgttgc ttcaagattt tgtttgatag catcaatcaa tttattcttt tctttttcaa
    24241 aatagaacac aggtagttgg gggtattttt ggttcaattc ttcttttagt gcatgcattt
    24301 gttccccata aaggaaaatc atggcgaatt tctcaggatc gatatgttca ctcatttgcg
    24361 catgcatatt taaagactct ttccctaatt ccagcatatc tgctaacaca gcgattctgc
    24421 gtccttttgt tggcagattt ttaaaagtat ctaataccag tcccatggct gttggatttg
    24481 cattgtagac atcgctcaaa atagctgcac catttcctgc cgttagccat tcggttctgt
    24541 tttgagtgat agaaacatgg gcgagccctt ggcgtatctc aggtatggac agtccaaaga
    24601 agcgcccgat cgtatacgca atcaaagcat tcgttacatt ataacttcca ggcagcggga
    24661 tcgtaaatgt ttctccttct actacaaaat cggtttgtgc ttttcctttt gctgtcactt
    24721 ccgcatgaac atcgccattt ccaataccga atgttgtgac tgtttgtact aatgttgccg
    24781 ttaaaggtgt cagcaacggt tcatctgcgg gcacaacgag taatccgtct gatacaagtc
    24841 cgtcagtgat ttccattttg gctttagcga ttccttctcg cgacccaaga ttttctatgt
    24901 gtgcttcacc gatcattgtg attgctgcag cttttggttg agccataagt gaaagttccg
    24961 tgatttcatt ggcgtgatcc atgcccattt ccaaaacgat cttttctgta tcttggggca
    25021 tgtgcaaaat cgtgtaagga agaccaatat tattgttgta atttccttgc gtcttatatg
    25081 ttttgtattt ttcagacaag acagacgcgg ttagatcttt cgtggtggtt ttaccattgc
    25141 ttcctgtaat tgcaacgact tctgcgccta ctttttccaa gtaataggtt gccaattgct
    25201 gcatggcttt caaaggatct gctacaggaa taactggaaa attttttgga gcttgttcaa
    25261 caggcaagct ccaaaatgaa gcagatgctc cgtttgtgat cgcttgctgg ataaattcat
    25321 gtccatctcg cgtaccagca agaggaacga ataaacttcc ttcggttatc ttccggctgt
    25381 caaattcgac attggtaatc tttgtcagtg cagagcattc gctgcccacg gcttgtgcaa
    25441 tttcttgtat tgttagattc attgagatct ctcctaaaaa aggttgtgaa aaaagcgttt
    25501 ggcctaaacg gcgggttcgc atgactctca cttccaagta aatttctcta ttcgtattta
    25561 gaagctagaa tgaacgcggt tacgcaatca ctccagcctc ttctttattt ctctttattc
    25621 atttttttca aaaaaagatt gacgctgatg gtatcgattc attcctaact ggatcagttc
    25681 ttcaatcaaa tcaccgtatt tcaagcccat attttcccat aagagtgggt acatactgaa
    25741 ctccgtaaat cctggcatag agtttaattc attcaggaat aattcatttt tatttgtcaa
    25801 aaagaaatcg caccggctca atccgcttcc acctaacatc gtgtaagcta acttcgcgta
    25861 ctcttgcgct ttttgataaa cttcttctgg cacttcggct ggaatctgca tttcgatttt
    25921 attattgata tattttgctt cataatcata gaatgctacg tcttttacga cttcaccagg
    25981 caaagtcgtc cgaacgtctt catttcctaa tacagcaact tcgatttcgc gcgcttcaat
    26041 tccttgttca acgattgctc gagaatcata ctgataggct gttgctaaag cattttgcag
    26101 ctcttctcgg ttttctgctt ttgtaatgcc gacactagaa cccatattcg ccggtttgac
    26161 aaacatcgga taaagcaaag aaccttcaca ttgatcaaat acttttttag gattttcttt
    26221 ccattgattc ttaagtactg gtacataagg aacttgcggc acaccagcag cttgtaaaat
    26281 atacttggtc atgattttat ccatggcaca tgcactggtc aatacgcctg cgccgacata
    26341 aggcatattc aatgtctcta agaagccttg gatcgttcca tcttccccgt ttggcccatg
    26401 taaaactgga aaaacgatgg ctccttcttc tttgatttcg cccggattga tcacttttcc
    26461 tgtaaagcct tcctctgtct gtccacttgg gtcccatgaa agatgcaaga catctttgct
    26521 ggcaggtttt tctgttagta atggaccttt gacccattgt ccttcttttg taataaatac
    26581 gagttgaact tggtaataat tataataaat ggcatttaaa actgaaaatg cggaaagaat
    26641 ggacacttca tgctctgcgc tgcgtccgcc atatagtaaa gtaatcttca aaagattgtc
    26701 ctcctgtatt ctaaatcgtt cagtgattta ctctcatatt ttagcacaaa acaaaaataa
    26761 acgaataact tatttataaa aaaacgagcc agcttctaaa agaacgagcg tcatttattt
    26821 tttgtcagct agattatttg tcccgttttt tataaaaagt tatgataaag aaaaggaggg
    26881 atgatgatgg cgcaaacaaa tccacgcagc cgcttggcag aaatcattcg agtgttcatc
    26941 aattacaatg tagtgccaaa ctttgtacag caaaaaaatc ccgaacaggt aaaaaaagcc
    27001 tttgaagaac taggccccac cttcatcaag ataggacaga tgctttctgt tcgagaagat
    27061 ttgctttctt ctgccttcac tcaaacattc aaaacattac aagatagtgt acctagcgac
    27121 acattttcaa cggtcaaaaa gacgatcgaa acagaacttt ctctttcatt atcagatata
    27181 ttcgatgatt tttcaaaatc gccttttgcc tctgcttcta tggggcaggc tcatcgtgcc
    27241 aagctgaaga acggtgacgc ggtagtcgtt aagatccaac accctaacat cgccgaagag
    27301 atccgacttg atcttcaact atttgaacga gcgatccctt tgatcaaata cattcctgaa
    27361 actagcgtag ttgatctaaa aggggtactc caagaagtaa aacgttcctt gatcaatgaa
    27421 atggacttct taaaagaaag tcaaaatggc gaacaattct atcaaaagaa caatggctgg
    27481 aaagagatcc gcagccccaa gatttatgac gctttttgtt cgaaaaaagt gatcgtgatg
    27541 gaagaaatgt ctggtaaaaa cttgaatcat ttaatgaacg ccgaaaacaa aactgaaact
    27601 tttatcactg gaattcaaaa caagcagctg aaacaagaag ttgcaaagct tttagttgag
    27661 aactttatga aacaggtgtt tgatgatggt tttttccatg ctgatcctca tccagggaat
    27721 ctgctattcc atgtactaac aaaagaagaa cagacacaag ctagccgaaa aactgaaaca
    27781 gtccatgaaa aagagttcgg ttcctttgct tttcgagcct ccacttctgc agaagaccct
    27841 gttgcgccat ataccatcaa ctacatcgat tttggtatga tggggcatct ttctgcagga
    27901 cttaggcaaa aactcacaca agccgtttta gcgctgtata cgaaagatgc ttatcgaatc
    27961 gaaaaagcgg tccttcgact ttgtcagcaa gaaggctcgt ttgatgaaag tcggttccac
    28021 caagaactga caagttttct tgaacagtat tatgattctc ctattgatga aatcaacttg
    28081 caagaagtat tcacacatgt cgtgacgatt tgtcatcaaa ataatctgca gttcgatcgt
    28141 gatatcacgc ttttgctgaa agcatttggg acattagaag gggtgatccg cgtcctcgat
    28201 cctgaagttt ccctcatgga agtcgcttcg ccttttgctc aacattattt tctcactcat
    28261 ctggatgtag aagatacatt gaaacaaagc ggattagact tattggaagg gatgaaggct
    28321 gcgcctaaga tccctcaaca gctccaccat ctattagaaa tgtggacttc gggacaagga
    28381 aaagtcaatt tggaattgaa aaagcaagac aaattattga gcaggatcga aagtatgatc
    28441 aatcggcttg ttttcggtat gattttagct gcattgatcg tagggtcctc cctgcttgtc
    28501 caagcagcac ctgtagaaaa cgcagaggtc gtttccttat tagggatatt tacgtatgct
    28561 attgccgcat tcgtcattat ttttttggca atcgatgcat tgatccaaat gtataagaag
    28621 aggaaaaagt agaaaaaggt tgtgacagaa agaatcagct tcaagaaata agaaggggtt
    28681 ttacataaat tgtttctcaa atttttgtga aaattcggct tatttttcga agaggctact
    28741 tctgaaacac cgagaataaa aaatgtgaaa aagctgttct gcacccagta ataagaacaa
    28801 tcaaaaaggg aacatggaac acatttatcc tgtttccctg ttcccttttt gatgtattcg
    28861 agcataaata aaataccttc gttacctact atcttctgta cttggtaaac cttagtcaat
    28921 tgcctttttt aacgcttgac gaaaagacat atgatctttc tccgtaaagg gttttggacc
    28981 ctttgtcctt atgccagctt ttcttaactt tccactatga taacgttgtt ctagaagcgt
    29041 gccgatattc gcttcgtcaa attcttttcc agagtgatgg aacactcgca cacccttcgg
    29101 caatacaagt gaagctaaac cataatcttg cgtgatcaaa aagtcattct tcttaaccat
    29161 accgacgatt ttataatccg cactatcagc tccgcggtct acgtagacga tttggacatg
    29221 attagcgtat tctttggtcg tataatggtc cacactggtc acgataacca cgtcaagacc
    29281 ataggattct cctaatttta tgacatcttc ttttactgga gaaccatctc catcaatgaa
    29341 tagcctcatt ctttttcgcc ttcttccata aatgataaac agttactaat ccgctcagta
    29401 aaagaccaac attgatccat tttgccaaga taagcagttg tacactttct atcggcactg
    29461 attcatggtt cagcgtgatc aataatagat ggattggctc tcccatttct tttgctaaaa
    29521 caaacaaaag cgtcatcaat aaaaactggt ggatcacact ccctttattt tgagtagtat
    29581 acttgacaac gatccggcga tccattggaa tagctgtatc agtttgataa tattccttat
    29641 ttagccagct gcctgttact acgtgccaga aactaagcag aaagacacct atcgcaaaac
    29701 catttggaat caagaggacc aataaagaag aactcagtcc aagcaattgc atagccaatg
    29761 gagaccatgt taaacgtttc aaaatccatg gtccgactat ttttgctgca atcaagcctg
    29821 ctgcataagg caaatacata aaaatcgaaa gtttttctaa tccgtacagt atgccaacat
    29881 aaaaactgct aaataaaaaa aggaaattcc ctagcattcc atttagaaaa gtaaacagat
    29941 ttagccagct tggcaacttc cattccttct tctggtgtcc taaaacaaac acgaacaaga
    30001 tgaacagcag caagaaacca taaatagcaa gatcaaaaag cctttcatcg aataacaatc
    30061 gggcatttct caaaagaaac aataagccaa agaatatcaa aaataaaaca agctctcgcg
    30121 tagctaccag atttcttttg atgtctttga aatccagttc gtaacgcgga taatgactaa
    30181 ccgtatggta ggccatgacg taaaaaagtg tataaatagt aaagcttgct gcaatctgag
    30241 tcggtgcagg caactcaatc gcttgataca ggagaaatag ccagatagct gcaaataagt
    30301 actttttccc atccatattg ataaaacctt gcgtcttttc gtgaaaattg accgtggtat
    30361 tagcaggtac aagccatgct gcactcagcc ctagcaaaac acttgaaaaa taaaataaag
    30421 gaaaataaaa tacgcctaat attccaagca atgaaccagc accaccaatc agcagagcga
    30481 tcatcagcaa cgtgtaacta tctagaccaa atcgtagact gttgactaaa aatagtccag
    30541 tcatccgaaa ggcataaaac agcgtaaatg gcaaaatcgt cagccaatta tgcgagtctg
    30601 ttagtcccaa aaataaaaga taaggaataa aaataaccgt gttacttaag ataaagggcg
    30661 ttgtttgtgt aaaattacgc ccaaatcgtt tgagtttttc gaacatgaca atggctcctt
    30721 aaacagtctt ataactaatc tgctcttcga ttttagcatt aaaggggaaa taaaccaatt
    30781 ttacagaata tattcttgta aaaaaaaata cagaaagaag cataagcttc tttctgcatt
    30841 tttacggtac ttgattactc aagcagacaa gcccttctcc acaaaagtct tgcccgacac
    30901 ccctgcatcc ggcaggtgat aaagatagaa atcaattatc ctgtgcctcc aaaatcaatt
    30961 aggagctctg taaaccagag tccttctcga cccgtcgcat aaatttactt taacacagtt
    31021 cctatgttta tgcgagagct tttttcattt tataatcgac catttgaaat ccttgaccga
    31081 tatctgaaat aagttcttct gtaatctcaa atccttcatg cttataaacc tcgattgctt
    31141 gatgattccc ctgattcact cgcaaaaaga tctctctttc ctttgtttgc gcagcaagtt
    31201 cttcatacca gcgaaaaact gaagaagtca atccttgacc acgtacttta tttagcaaat
    31261 ataatttact taaataaatt tttccattta tctcttcata agcagtgtaa ccgacagtct
    31321 tttcttcgct ttttaataaa aagtaggaca caccttctgt cagttctttt tgaatctgtt
    31381 tttttgactg ataagttttc agcatgtaat ccacttgttt ttgaccaatg atcggcacaa
    31441 aaacttccgg ccatacttct tgtatgactg ccatcagctc atccagttca ttttctgtta
    31501 cttttatttt ttccatacac ccacgtcctt ttttacatta taaatgacct tgcgcttgat
    31561 ggaataaaaa gagcatgatc atgctataca cacccaccga ccacggtttc aagaaaatca
    31621 caatctgcat aaattcccta aaagataatt tcgtcaaacc agctactaga cagatcaagt
    31681 cgtctggcgc aaagggaagc agcaaggtaa caactagtac ttttttaata tctttcgttt
    31741 ttcttgtcag caattcatca aatttctgat acttttttgg cgataaaata agctggacaa
    31801 aggctacgcc atagtaacgg acaagcaaaa atccgattgc ttccccaatc actaggccaa
    31861 tacaactata taaaagccca aatccattgc caaacatcag catgcccgct actgaggaga
    31921 tccctcccgg caaaatagga atgatcactt ggatcacttg caacagaaca aatatcatca
    31981 ctgcatactt gccaaattgc tggataaacg cttgcaaaga cgtgctcgat cgaaaaatcc
    32041 catgatgata agcataaaga atcaacacaa agaaaaaaaa cagaccaata atgggcatga
    32101 tctgaagaca tttttgtaaa actgatcctt ttttagacat ctatattccc cacatctttt
    32161 ttcatttatt aaaactgcct cct
//
LOCUS       DARBEC010000023        32178 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.023, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000023 DARBEC010000000
VERSION     DARBEC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 32178)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 32178)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..32178
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.023"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(407..766)
                     /locus_tag="SCL80_001020"
     CDS             complement(407..766)
                     /locus_tag="SCL80_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEG1106906.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            complement(796..1137)
                     /locus_tag="SCL80_001021"
     CDS             complement(796..1137)
                     /locus_tag="SCL80_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106907.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            complement(1134..1808)
                     /locus_tag="SCL80_001022"
     CDS             complement(1134..1808)
                     /locus_tag="SCL80_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1106908.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCL80_001023"
     CDS             complement(2984..4282)
                     /gene="asnS"
                     /locus_tag="SCL80_001023"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEG1106909.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            complement(4322..5512)
                     /locus_tag="SCL80_001024"
     CDS             complement(4322..5512)
                     /locus_tag="SCL80_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1106910.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            complement(5533..6060)
                     /locus_tag="SCL80_001025"
     CDS             complement(5533..6060)
                     /locus_tag="SCL80_001025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEG1106911.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            complement(6107..8896)
                     /locus_tag="SCL80_001026"
     CDS             complement(6107..8896)
                     /locus_tag="SCL80_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1106912.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNGKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            complement(9043..9243)
                     /locus_tag="SCL80_001027"
     CDS             complement(9043..9243)
                     /locus_tag="SCL80_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1106913.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(9696..10016)
                     /locus_tag="SCL80_001028"
     CDS             complement(9696..10016)
                     /locus_tag="SCL80_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106914.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(10519..11637)
                     /locus_tag="SCL80_001029"
     CDS             complement(10519..11637)
                     /locus_tag="SCL80_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106915.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            12108..13415
                     /locus_tag="SCL80_001030"
     CDS             12108..13415
                     /locus_tag="SCL80_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEG1106916.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            13536..14735
                     /locus_tag="SCL80_001031"
     CDS             13536..14735
                     /locus_tag="SCL80_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEG1106917.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(14762..16030)
                     /locus_tag="SCL80_001032"
     CDS             complement(14762..16030)
                     /locus_tag="SCL80_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEG1106918.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGIAFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(16200..16841)
                     /locus_tag="SCL80_001033"
     CDS             complement(16200..16841)
                     /locus_tag="SCL80_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEG1106919.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(17250..17753)
                     /locus_tag="SCL80_001034"
     CDS             complement(17250..17753)
                     /locus_tag="SCL80_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1106920.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(17876..18640)
                     /locus_tag="SCL80_001035"
     CDS             complement(17876..18640)
                     /locus_tag="SCL80_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEG1106921.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            18748..19023
                     /locus_tag="SCL80_001036"
     CDS             18748..19023
                     /locus_tag="SCL80_001036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEG1106922.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            19583..20533
                     /gene="yidC"
                     /locus_tag="SCL80_001037"
     CDS             19583..20533
                     /gene="yidC"
                     /locus_tag="SCL80_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEG1106923.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPANNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(20579..20980)
                     /locus_tag="SCL80_001038"
     CDS             complement(20579..20980)
                     /locus_tag="SCL80_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1106924.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(20994..21803)
                     /locus_tag="SCL80_001039"
     CDS             complement(20994..21803)
                     /locus_tag="SCL80_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1106925.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(21790..22680)
                     /locus_tag="SCL80_001040"
     CDS             complement(21790..22680)
                     /locus_tag="SCL80_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1106926.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(22693..23172)
                     /locus_tag="SCL80_001041"
                     /pseudo
     CDS             complement(22693..23172)
                     /locus_tag="SCL80_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(23348..24289)
                     /locus_tag="SCL80_001042"
     CDS             complement(23348..24289)
                     /locus_tag="SCL80_001042"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEG1106927.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCL80_001043"
     CDS             complement(24286..25281)
                     /gene="lacD"
                     /locus_tag="SCL80_001043"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1106928.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(25274..26470)
                     /locus_tag="SCL80_001044"
     CDS             complement(25274..26470)
                     /locus_tag="SCL80_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1106929.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(26488..27216)
                     /locus_tag="SCL80_001045"
     CDS             complement(26488..27216)
                     /locus_tag="SCL80_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1106930.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCL80_001046"
     CDS             complement(27657..28631)
                     /gene="lacD"
                     /locus_tag="SCL80_001046"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEG1106931.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(28628..29086)
                     /locus_tag="SCL80_001047"
     CDS             complement(28628..29086)
                     /locus_tag="SCL80_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1106932.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(29083..30546)
                     /locus_tag="SCL80_001048"
     CDS             complement(29083..30546)
                     /locus_tag="SCL80_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1106933.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCL80_001049"
     CDS             complement(30528..31460)
                     /gene="pfkB"
                     /locus_tag="SCL80_001049"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEG1106934.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            31602..>32178
                     /locus_tag="SCL80_001050"
     CDS             31602..>32178
                     /locus_tag="SCL80_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1106935.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         9385 a         6921 c         5511 g        10361 t
ORIGIN      
        1 ttcttccata cagatgtttt ggcttattct ttctttttct cttcgatcat tgcccctgct
       61 cttatccaac ccgcacttgc cttcttgatc ttgacaggat agcaactaag tttgaagcct
      121 gttgctggca atttgtctaa attcattaat ttttcgatag gacaatatct tttttcaatc
      181 cctgcaaaat gtccttccca gatattcgaa gctccactac gctttcaaaa atatagaaag
      241 agttctttaa tgaaaattat ttcaactttc cataaacagt aatcccatat aaataaaaga
      301 cgtaaaagtc acatgggaga acttatacag gaaaacacat agagtcaggt gaaatacatg
      361 tatcccacct gactctatgc gttttctaac ctttattatt ctctttttat acaatttctg
      421 caccgaatgg catcaacgcc aatcgagcaa gtttgaaaga ttgcttggca aaaggtatcc
      481 ctatgatcgt gatacataga aggatgccgc tgacgacatg tgcgagagca attggtaggc
      541 ctgaaacgat cagccagatg atattaacga tcaatgacat gccagactgg tgataaatta
      601 cttctttgcc aaaaggcgca aaagaaaggg aggcgatctt aaaacattgc ataccgattg
      661 ggatacctat gatcgtgata caccaaagta tccccgctgc taaccagcta agaccaccga
      721 ttagtccgcc gaatataaac cagatgacat ttcctaaaca actcattgta tttctccttc
      781 tattactgtt tttgattaaa actgggcaaa aattgatttt attcttcttt ttttagcttc
      841 cagcatcaag tattctgcaa attttttttg gatttcttcc gtattttctt ttgttactaa
      901 tgtgtgttgg attagttttc ctgatggtcg ctgatccacc cgttcttctg actgttgcac
      961 gtccgcttgc gcaaatccga taatccgttt ttcttttgtt acagcaacga gcaatagttt
     1021 attcttatca tttaggtcag ctgccgtttt tgcttttacg aatgattctg gatgatcagt
     1081 aatcagctga ttgatttgag gtaaatcttc ttttactgcc caccgtattt ccatcatttc
     1141 tcacttttct ttcgctcatt tttatctcca ctaaaaaaat cgactgcggc aattgcatct
     1201 atccagcttt gtgcaatgat ctcatgtccg ccaatcgttg ggtggacccc atcttctctt
     1261 gtatatttcg aaaaaccata agcaatccct aaagcattga atatgccatc taaaggaacc
     1321 agcaatgcgc catattcctt agcaagttta cgaacaattt ggattttagg gtctaagtct
     1381 ttacgccagc catgacggtc gtcggttata ggaagtacaa acggctccat caagatgatg
     1441 ttgcagattt ccgctgtttg aagttcattt agtaattttc gataaacgga ttcaaattgc
     1501 tgcgcagatt tctcagttcc gaatatagcc tgatcgctag tattatgcca ggtatcattg
     1561 attccgatca aaatcgatac atagtcaggt tgtaaagcta aacaatcttc ttgccagcgt
     1621 acttgcagat ctgcaatttt gtctcctcct atcccacggt tcatgcattc aaatttcatc
     1681 gttggatatt gggagattag ttttccggca atcaaaagtg gataaccttt acctagatca
     1741 gttgcttctg ctcggtctct tcctgcgtct gtaatgctgt caccgataaa taatatacgt
     1801 tttttcattt gtcaaaaact cctttttgaa taaaacgatg attttcttcc ttatattatc
     1861 ccataagccc tatcaagacg aaagagaaaa tatatgtata aaaaatgaca ccagcctagt
     1921 cccttcaatc aattgaatga acgctcatca agtgtctagt tttttatact ttttttctta
     1981 acgtgccgga ttccccttga ttccttcgtt gtttggtcca gagaaatcga tcacacctaa
     2041 gcttccgtca atcagaccac tgatgacgcc atcagaacat atatttatag gctcatctaa
     2101 gacttccagt aaatgttctt ttactaatat ctcttctaga ttgcttggga tagattggtc
     2161 ataactttat ctggtccgat gatttcaagg atgaattaaa cttacatatg gatcattgca
     2221 tgctgaaaat acggacctac tccatcttcg aatgttacta ttagtttgtt ttcaggtact
     2281 agatgctgct ttatttcaac agcagaagca tcatcaaatc agatttccat ttgaataacc
     2341 ttctttgtat atgtttgatt gattgcgatt tcgttataga accttgtgaa tcaaatagca
     2401 atttcttttg ctttcttctc ttgtctgctg gaaacttcct ttatcccgta ttttaaaaaa
     2461 agaactgcga gagttcgcag ttcttttttt taaataaacg ttgctctccg gccaactcct
     2521 cggtcacaag tcacaatagt catcaaacat gaaaaactgt tttttgaaat tactccttgt
     2581 gactcggagc tgaacggcag gcgcacaacc tcttgatata aacggtgttc aatcagctga
     2641 ccctcgatat tagctaaaaa aacggtaaaa tatgagaagc tatttttccg ttttgtttct
     2701 ctaatactca ggtcaaggca ctgatttcac aacctcttcc ttaaacggtg ttcaaaagcc
     2761 agtttctcgg tattaagcca aatttatgca aaaatgtgga aagccatttc cacaaaattt
     2821 ttcttaatac tcgaaactaa acagcttttt cacaacctct ccactaaacg gtattcaaaa
     2881 gcttacatct gcggtaataa gtcccgccaa ttcaaaaaca tgaacagctg tttttgcttg
     2941 gctgttctaa ttacttgatg cagaacagct ttttcatcac ctcttatgga taaattcggt
     3001 tcaacaaacg tgggaatgga atagcttcac gtacatggtc gataccagca atccaagtga
     3061 ctgttcgttc aaggcctaga ccaaaaccag agtgcggaac agaaccgtat ttacgtagat
     3121 caagatacca tgaatattct ttttcatcca aaccgtgatt acggatttgt tctagcaaga
     3181 aatcatagtc tgttgcccgt tcgcttccgc caataatttc accgtaacct tctggtgcga
     3241 tcatatctgc acaaattacc acgtcttctc ttgttggatg tggtttcatg tagaatggct
     3301 tgatggcttt tggatagttt aggataaaga caggctgttc gaatgaattc gcaatgaacg
     3361 tttcatgagg tgaaccaaag tcttcgcccc attcgatgtc atcaaatcca tttttcttca
     3421 gtaattcgat tgcatcatcg taagtaatac gagggaatgg caattttgta taagttttta
     3481 agacttcttt gtctcgtcct agtacatcta atgcatattc gcaatgatct aaaacatttt
     3541 gtactaagaa tgctacatat tgttcctgta cttctaaact ttcttcttga tgagtgaaag
     3601 ccatttctgg ttcgatcatc cagaattcga tcaaatgacg tcgtgttttt gatttttcag
     3661 cacggaaagt cggaccgaat gaaaagactt ttccgaaagc catagcagca gcttccatgt
     3721 ataattggcc actttgagat aaataagctt tttgatcaaa gtaatttgtt tcgaatagat
     3781 ccgtagtgcc ttctggtgca gaaccagtca agatcggcgg atcaattttg acaaaatgat
     3841 gttgattaaa gaattcatac gttgcacgaa tgatttcatt tctgatctgc ataatcgcat
     3901 gttgtcttga agaacgcagc cataaatgac gatgatccat caagaagtct gttccgtgtt
     3961 cttttggtgt gatcggataa tcgtggctct cgcttaccac ttccacattt gtaattccga
     4021 tttcataacc aaattttgaa cgactgtctt cacggatttc tccagttact aacacggatg
     4081 tttcttggtg tagattttta gctgtttgga aaacttcttc cgacacttcg ctttttacaa
     4141 ctacaccttg gaaaaaggct gttccgtctc ttaattgtaa aaatgcgatt tttccgcttg
     4201 atcgcttatt tgcgacccaa gcgccgattg ttactgtttc tcccacatgg tttttcgaat
     4261 cgatgatttg aatttgttcc acgtatgact ctccttttat attccttgaa tttcttcagg
     4321 attaattttg acttttactc tcaataaatt gcttgattct gcgtaccgct tcttctaacg
     4381 tagcgcgatc agtggcataa ctcatgcgga cattttctgg tgccccaaag ccttcgccag
     4441 tcacaagcgc tacccctgtt tcttctaaaa gatcttctac ccattgtgtg acattttcat
     4501 acttacacat gcgtagcgtt tcttttacat taggaaaaag atagaaagct ccttgcggct
     4561 tttcaagaga aacacccggc agttctgcca caagcggata taacgtattc aggcgttctt
     4621 caaaggcgtt ccgcatttct tctactgtat cttgctcacc tttcaatgct tcaacagctg
     4681 catattgact gactgctgtc ggattgctag ttgattggga agcaacagcg atcattccat
     4741 tgatgatcac aggatttccc accgcataac caatgcgcca gcctgtcatc gcatatgttt
     4801 tagacacgcc gttgatgatg atggtctgat ttttgattgc ttctgaaatc gttgcaattg
     4861 gagtaaactc attgccatta taaactaaac ggccataaat atcatcagca actatcaaaa
     4921 tatcattttt tactgcccat tcaccgattg cctgcagttc attcttgcta taaatcattc
     4981 cagtcggatt cgatggagaa ttgatgatta cggcttttgt tttttctgaa cgtgcttgtt
     5041 ctagctgttc aacagtaact ttccagctat tggattcttc ccctttcaca aagacaggaa
     5101 gtccttctgc cagttttact tgctcaccgt aactgaccca gtaaggcaca ggaatgatga
     5161 cttcatcttg cgggtcaagg atcgtttgga aaagggtata caaagcaaat ttcgctccgt
     5221 ctgttacgac tgtttgtgaa ggttcataga caagtccata gtttttcttc agataatcaa
     5281 cgattgcctg tcgaagctct gggattcctg ctgttggtgt ataatagcta gctttcccat
     5341 tgcggattgc ttcgattgct gcttcttgga tgttttctgg tgtcgcaaaa tccggttctc
     5401 ccacagtcaa actcagcaca tctctgcctt ttgcttttaa tgccttggcc tttgccgcag
     5461 ctgccaaagt gacagacggt tctaatcgtt ttgttctttt tgataactcc atcccattca
     5521 ttcctttacc ttttaaattc ccttgattgt cttgacttca tctccagatt caaagccaat
     5581 caggtaataa ttcagtttcc catttgtttc ttttcctact acttcccaca caggctgatc
     5641 atcatacatc cctaaagcag ctttttcgat ttggatttca ggatgggccg cagcaattgt
     5701 ctgttttgct tcgttttcag tcagtccctt tgtttgatcc aatactttta ctttttcacc
     5761 agatttagga atgataacgg cgatttcttt tcctttatca tttgtacctg tcagtgaaaa
     5821 gtatgtcttc tttcgagtaa accaataaaa atgatcggtt tcctcaagat ttgcatattt
     5881 ctttgcgatt tctactgctt ctttcttcgc ctgcaccgcc ggacgactgg aacgcaagta
     5941 aaaaatcgag ctgatcacga tgaccgctaa taaaaatacg atcaagccaa gtaacacgcg
     6001 attaatcgtt ttttcctttg tctgtttttt cttcaagggt cgtcgctcct ttatcttcat
     6061 tccttttcat tatagcagat tttaattatg aaaaaagaca gctcctctaa cttccttctt
     6121 catttttctt taagaattca tggatatcct gatagatttc ctccatagct agctctttga
     6181 tcggtacttt tttcggtaat gcatggatga tccgtgcgcc atattttgca gtgactagtc
     6241 ttcgatccaa aataagcaat acgccacgat ctgtctccgt ccttatcaag cgacctaatg
     6301 cttggcgtaa cttcaaagct gctttaggta cggattcctg gtaaaaagga ttgatcccct
     6361 ctgattctaa ataagcgttc ctagctttga ccatcgggcg aagcggatta tcgaagggta
     6421 aacgtgtgac tacaacgatc tgcaaagcat ctccaggcaa atctacacct tcccaaaagc
     6481 tgtcagcgcc aaacaatacg cttttttctg acaaaatgaa gcgtttcaaa agcttctccc
     6541 ggcttccacc aaacccttga gctagtattt ctcgtccttt ttctagcata gacagatgca
     6601 ttttctgata gaccttctgc aaaacatcat gagaagtgaa taacacaagt attggccgat
     6661 cttcttgttc gatcagacct gtaagtgctg tctctaaata atcgatatat gcttgattgc
     6721 tgacttgatg gacggcaggt gcctcttttg gcacatagat ccttgcctgt ttctcataat
     6781 cataaggaga ggcaatcact ttaagaggtg ctgcttcaat gccaagcctc ttagagagat
     6841 agtgtcgatt agggccgatc ttcagcgttc ctccaatata aagaatacga tcatatcgag
     6901 gataccaaac agtcgacggc aacatagccg cttgaaaatc atggagatga aaaatcatct
     6961 gctgcgctgt gttcccagga acgaaccaat ggatcaaatg atctcgccag tcttccagcc
     7021 attgatccat cagcaagtat tgcttgtcta attgatcaaa taggctatat aaacttgcta
     7081 aatcgatccg catctggttt gtccagcttt cttggataat ctcaaaagtc agacgcattt
     7141 gtgactgtag ctgctggatc tcctgataaa aaagctgcag gtgatcaatg gctgtcccag
     7201 attccagtgg cagctgatcc aacatctctt ttgtcatcat gacttcttct ggatactgtc
     7261 cgttaggttg cttgccgctc actaaactca ttaatccttc tgccatacaa atctgtgctt
     7321 cgcatagcgc agaaagttct tctcgataaa tcgataacca gcgtaaggct tcatgttctt
     7381 tcggtaaata gctttgtatt cggtcaaaca gctgatttgg ttcttctaat tgctggattt
     7441 gtttatggaa atagttaata cttaatcgtt tgccatttac tttttcagcg atttccggca
     7501 aatgatgtac ttcatcgatc aacaaaaatg gactagctgg aagcaatggt tcttttcgct
     7561 gtgtttcttg tactaaaaac gcatgattga caatcaaaaa attactttgc ctgattttct
     7621 tttgcaagaa acgaacaaag tcttgttcgt aaaatgactg atttcttgct aaaaatccaa
     7681 tacctctgtg cgcgatatct gcaaagaatg aatggttcaa atttgttaaa tgtaattcat
     7741 ccaaatcacc agtcttcgtt tgcgtcagcc aaactaagac cgccatttga tacaaagcat
     7801 attgtttttg ttctggcggt tccacaagtg tgcctttaaa acgctgcaaa tcaatatagt
     7861 ggcgatagct tttgataatt acaggttgta tgggctgatc tagcaattga ttcagcaatg
     7921 gaatgtcttt ttctatgatc tgctgctgca aaactagcga aacggtactg atgacagcgg
     7981 gtttttcagg tgtagccaaa taactcaatg gcagcagata accgattgtt ttacccattc
     8041 ctgtagctgc ttcgatcatc aaatcttttg attcatcttt tgtgaaatgg tcgtagaccg
     8101 cattcatcag tctgttctgt tcttttcgat acatcagtgt tttaccaaac attttttctt
     8161 ttgcttgttt ttttcttgga tatgtccgct cgccataatg gacggatgtg aatagttcca
     8221 cttctttttt ctgcaaagct agcccatcga cgatttctaa tgagggatca agtggctctg
     8281 gtttttcttt catttcttca atcacatgat aaatgaaacg actcgtatcc atacctgtgt
     8341 gtctacttaa tcgcgcaatc tgctctaacg tgatgattgg caattttctc atccgttctt
     8401 cgatcagcaa aagaagttga cctgtcactt cggcatcact atctgcctga tgaggattct
     8461 catgaataaa tccaaggctt tctgacaaat cagctaagcg aaagcttggt tccgtcggca
     8521 agaaaatttg tgcaagttct actgtatcga tgcctggtat cttcaaacta ggaagtccgc
     8581 atcgcatgag ttcatggttc aaaaaattat aatcaaaata aatattatgc gcgacaaata
     8641 tggtgtctgc caaaagattg taaatcgttt gggcgacatc ttcgaaataa ggcgcttttt
     8701 ggacgcgttg attcgtgatc cctgttaaat gctggatttg ttttgaaatc ttccgccctg
     8761 gatgaatatc gatcgaaaaa cgtgaagtga tccgacctga ttcaatcatg acgcagccaa
     8821 attggatgat ccgatcttct tttggattcg tcccagtcgt ttcgatatca acgactgcat
     8881 aagttcttgg tttcaaaata tcttcctctt ctcctgcctc taatgttctt ttgaaattat
     8941 actacaaagt gcagaaaaat actctcatcg ctttttaaag aaaatagaca aaaacgccca
     9001 gacacgcaaa tgtctgggcg tttatttttt agtataggtt aattaagctt tagtaacgtt
     9061 tgttgcttga ggtccgcgtt ggccttcttc aacatcgaaa gttactgctt ggccttcttc
     9121 taaagttttg aatccgtcgc cttggatagc tgagaaatga acgaatacat cgtttccgtt
     9181 ttctgcagtg atgaatccaa atcctttttc tgagttaaac cattttactg tacctgtttc
     9241 cataataaaa atcctccttg tgtatcaaca catattttgc aattacttga atggtgtata
     9301 tctggaagat gtttatttga aacaagctac cacagattac cgaacaaaaa ctactcctat
     9361 agtatatcat accgtttaca aaaaagctag agtttttcga ctttttttat taaaaaaagt
     9421 tttctcagcg attacataaa gtgaaaaacc gaactttttt ttaaaataaa cgacatattg
     9481 ttcagttggt gtttttttgc tctgatgttc ttttttaaac taaaaaaatt attctttctg
     9541 actacttatc ggttctgttt caacggtcga ggctggggaa gattctttag taggtctgat
     9601 gttctcatgg tatttctgat agcctatgac agttaccaga agaacaaggc aaaccaatac
     9661 gaccaacgag attagttttt cttttttgct catatctata cctcataaat aacaggatga
     9721 tgtccatcat atttctgagt atgagtaaaa gcaaaagctg cagctgtaga atgtaagaaa
     9781 tgccagcaac tttcataatc acaggtcgga cagtaccaaa agtaaccaaa tcttggctgc
     9841 aaagaccgtt cttctgtttc aggaatcgca gaatcaagat ctggtgtctg tacataaaca
     9901 tttttgtaca gacgtgtgcc tgtaatagga aaccgctgtg tttcttcagc aaaaactggc
     9961 tgacttgtta aacttaggga tagagacagc aaacaccata acagtctttt tttcataaag
    10021 acctccttat cgataagttt ttcccaatta aaatcatatc tcaagaacgc ccgttattca
    10081 cattttgttc ataaatgatg ttttttgtat cataatcgtc aaaaagaggt cgtgaccacg
    10141 tcacaacctc ttttttaaac ggtgttccag aagtagcttc ttcggaaata agccgaaatt
    10201 tcacaaaaat ttgagaaata atttttgtca atctcttctt atttcttgaa gctgaccact
    10261 tctgtcacaa cctcttcctt aaacggtgtt caaaagcttg catctgcggt aataagccca
    10321 ccaaacaaaa aatatgaaga gctatttttg cttgctgttc ttattacttg atgcagaaca
    10381 gctttttcac aacctctcat acacggtgtt taatcagctg accttcggta ttagctcaaa
    10441 aaacggcaaa atatgtgaaa ctatttttcc gtttgtttct ctaatactca ggtcaaaaca
    10501 ctgatttcac aacctctttt aataacgaag aatcgctgtc agactctttt catctggcgc
    10561 atctttttga tccaaaataa acacttttcc gccattaatg atcacatcat ctgctaatgc
    10621 attcaacact tgacgccgat catattctgt ttcaggatct tcaccgaaac catcagctaa
    10681 gtttgacgta gcgataaata aatgtgacac ttttccttct ttcgcagctg gaacgatatc
    10741 aactaactga tcgatgaatt tcttatctaa gagtttattg tattcttttg attcttgagc
    10801 tgcgagttca cttgacagct tcttcgcccc ttcttcgata tctctaaagg atagctgcgc
    10861 aggagaagca gaaatcgcaa cgtctgcaca ataataggga tttttagcaa tttttttgaa
    10921 catcgtctga ttttctggta gagcaaataa ataaatcggc cattgttctg gattttcgaa
    10981 agaatccttc aagtacgtat caactgcttg gtaatagttg acccaatcga tctctacttc
    11041 ttcatctttt ggatttacgc catggaaagt tactccatct cgggctccgc ctcctccatt
    11101 agaagagaag ttcaaaccgc cacctgtcag ttcagatccc aatgcagtaa cgacatcaac
    11161 cggtgcccct tctggtagag atacttcttt tacttgtttg ttgctgactt catacaattt
    11221 catggagtcg cggttcaatc ccaataaata ataagaatag ttgaactgcc catttttgat
    11281 aatagccagt aaataaggaa gatcacttac atagtattga tcgtctaccg ggatactcaa
    11341 acgatggata taggtttggt cagcagttat aatgaccgct acactcgtag ttgcactacg
    11401 ccaaaacgaa ggatctccta acaaattttc aattttttct tggaacgggg accagtcatg
    11461 ttcggaatat tttttctcaa aacgtttctt tgcttctttc gcaaaatttt tgaatttgat
    11521 ctgatctttt tccacatctt gatgggcgac atgcgtattc agcatgatcg tgacgaatgg
    11581 tcctttagcg ccttctgaag tcaattgtga caataattct ttatcctttt ttgccataaa
    11641 cgtacccctc ctaaaaaaat cattcctttg tttctatcta aagtctacca caacactatt
    11701 tttccataca ataaaacgta tctaaaaaaa taaaacggtt tctacggttt taaatttagc
    11761 tattttttta agttatcttc ataatttcct caattattta gccccagtct tcaactattc
    11821 ttcatgaaat gctgataaac taagaaaaag aataaaagag gtggatctta tgcatttgat
    11881 tatgattttc agtttattaa gtattatcag tttatttgct ttcagcgctt cccgcttatt
    11941 agatgcggaa acacagccta ttcctgtaaa agtacgtaaa aacgaccgct aaccttccat
    12001 aacgtgcgtt cttatcctaa agttttttca gaaacattga aactctctcc cctctctgat
    12061 aaaattttaa tttgtagcga aacaaattaa ttacaaggag atcggatatg tccataaaaa
    12121 aaacacgtct agtcctatta ttggctagtc tcattctttg ccttgcttct tttctaccag
    12181 ctgttgctgt gcaagctgaa gatactttca aagtaaatgc aaaagcagcc tttgctgtag
    12241 atgcgcaaag cggtaagatt ctttatgatc aagatggaga aaaaaccatg gggatcgctt
    12301 caatcacaaa aatcatcggt ctttacatcg tccttgatca agtaaaagaa gggaaactat
    12361 catgggatga taaagtttca atttcagatt atgcagaaaa tttgagtgtc accccagatt
    12421 tatcaaatgt tcctttgcat aaagaaaaca cctacaccgt taaagaatta ttcgactctg
    12481 caatcatcca gtcagccaac gcttcaatgg ttgccttggc agaaaaaatc tctggcagcg
    12541 aagcaaaatt cactgaacga atgaaagaac aattgaaaga ttggggtatc aaagatgcaa
    12601 cgatcgttaa tgcatcagga ctgaataact cttatcttgg cgaaaaccga ccagaaggta
    12661 ctggagaaaa cgacgaaaat caaatgtctg ctcaagatgt ggctatcgtt gcccgtcatt
    12721 tgatcttgga ttttccagaa atactggatg tatctagcac tacgacacaa atgtttggcg
    12781 aaaacacgca atcaccagta gaaatggtca actggaactg gatgctccca ggattcgtga
    12841 attataaaga aggcgtggac ggattaaaaa ctggtacgac tgaacttgca ggagcctgtt
    12901 tcgtcggtac gatcactaaa aacgatcaac gaatcatcac tgtcgtattg aatgcagacg
    12961 gccatgcgga aaacccaagt gcccgtttca atgaaacagc tcgcttaatg gattattgct
    13021 acgacaattg gtcagaaaaa gagcttggaa aagccaatgc aagtatccct agtctaaaaa
    13081 ccattgatgt aaaagacgga aaagaatcaa cagtgcccgt tgtgcttaaa agtcctgtaa
    13141 aagtatgggt acggaacgat atggatactg gaaaattaac aatcacacca actcttgaca
    13201 aaaaacaagt cactgacaat gagatccaag cacctatgga taaaggaaca aaaatcggta
    13261 ccgcaacgat caccttggca gaagataaat taggttatct tgaagacaac aacgcgccat
    13321 ccacagaaat catcacggat aaaaccgtag aaaaagcaaa tattttcgtt attggctggc
    13381 gacgcgtcgc agaatttttc ggtaacttgt tttaattgaa caaaaagaaa aaagcatctt
    13441 tccatgtgga agatgttttt ttgctttttc tagtttaaat aacagctttt ttcatctatt
    13501 ttcgttatac tgtataagaa aaaggagttg aatttatgaa tctaaccaga gatctgattt
    13561 ttttggttct ctatcttatt ggggtattct ccatcgttta ttggcgaaaa cagaaaaagg
    13621 atccttcctt gttcttttac agcagtacat ggacgtttgc ggtaatcttg acttattttg
    13681 tcgtgttgga aattttttat tcgacctttt attttttgat tcccttagtg tttttcagta
    13741 tctttgcctt ttcctatttt tcagaaaagc gaaggctgtt gaatggtctt ttattcaata
    13801 ttttcctcat tagttttggt atctacttgt ttgtcttgct atacgaaaca caaaatattt
    13861 ttcttggcgg actgattgcc ttgatcacga tcccgttact gcttgtcttt ctattcggca
    13921 tttatggatt gatcgttttt cttttttgga acggtgtcac agttttacgt agagagtcgc
    13981 attcattagc taatctttta acgttgatac tagccatttt tcttacattg tttcttgttt
    14041 ttgacttttt cttgttaaaa tatttaccac aatggataaa tgctttgttt ttctgcatcc
    14101 cattgatctt gatctatcta tttatcgtat tctacaactt tttgactgtc tcattcttgt
    14161 atcagttcaa ccgccctcga tacaaccaag acttcatcgt tgtgttaggc gccgggttga
    14221 tcaatggaga aacagtttct cctcttcttg ccaagcgaat caataaggcg attgcttttt
    14281 attgggcaca aagccgtgcg acattaaatc cgcctatcct attaatgtcg ggtggacagg
    14341 gagcagacga aaaagtacca gaagcaatgg cgatgaaaca atatgccatg gaacaaggaa
    14401 ttcctgaaag agatattctt gtcgaaacca attcgacaac cactttggag aatatgcgct
    14461 attcaaagga aatcatggat cagcaaatga aaggtcccta tcgcgcgatc ttcagttcca
    14521 acaattacca cattttcaga gctggtctct atgcacgaca agcgaaactg aaggcaaatg
    14581 gcattggggc aaaaacagct ttctattact tgcctaacgc gtttttaaga gaatacatcg
    14641 ccatcttgat gatgaacaaa aaaagacata tgatcatttg cggattgctt ttcgcaggtt
    14701 ccgcattctt atcactgatc actttacttt tctaaaaaaa caaggtgtga caaaacatat
    14761 gtcacacctt gtttttttct tttattctct tgacgattgg caagaatagt attccttttt
    14821 tgacggctac ttgctgtgca gaataaatac cgctgtttcc tgaacacata aagctgatca
    14881 agcagatcat aaagaagtaa gcagcagctt gtgaaccaaa taattcgatt cccatcacaa
    14941 aacaggcaat cggtgtattt gtcgcaccag aaaatacccc gataaaacct aatccagcaa
    15001 gaaaaggaac agaaacatgg agcaaaggcg ctagtactgc gccaagcgtt gcaccaatct
    15061 caaatagtgg tgtcacttct cccccttggt accccgcacc taaagacaag accgtgaaga
    15121 ataatttccc aataaaatca taagcatgtg cctgtccatt aaatgattcc tgcagtaacg
    15181 gaagactcaa cccaaggtaa cgttgtgttt gcaaaatcaa cgctgcggct acaactactg
    15241 ctccacctat cgtatttcgc caaacaggat gaggaatcca ttgcgcatac cgcgctttta
    15301 aaaagacgat cgaacggctg aatacccaac caatcaaacc aaaagcaatc ccagcaacaa
    15361 aaactttgaa gaacagcaac atactccatt caggaatcac acccatttgg taatgtagat
    15421 gagaaacacc atagctttct gtcatgaaat ttgcaaaaag cgcagcaaag aagctaggaa
    15481 atattgcttc tgttcggact ttaccgattg ccaacacttc taatccaaat aatgttccag
    15541 ctaaaggtgt gccaaataca gaactaaaac ccgcactgat tccactgata accaaaatct
    15601 cccgttctgc tttatccaat cggaataaat gcgctatatt atcagctaat gctcccccca
    15661 tttgaactgc tgttccttca cgaccaacag atcccccaaa taaatgggta gtgatggtcc
    15721 caaacaaggt caatggaatg agccgcaaag ggatcttctc ttctcctcca ttcccttgat
    15781 cgatcactaa gttattccca cgactagaaa gaccgccatg atacgcataa agataagcaa
    15841 aaagcgcgcc gctgactggc aatagataaa gtagccaagg atgtgctagt cggatatctg
    15901 tcacgaacgt caggctattt aagaaaaaag cagacaagct ccccatgaat actccgagaa
    15961 tcgctgtgac caccagccac ttcaacatgt acatcaaaat acgcactgga ctcggtaatt
    16021 gtttttccat aaaaaattcc tcctcaaata aaaaaacgcc tactaaagaa aggggctggc
    16081 cccagacttt agtaggcgtc attagctatc ttacgttagc atttaaaggc gaacaccatc
    16141 acctattcac ttccattaaa agatagcata tttttctgta aaaaacaata aaattatcgt
    16201 tatttcttta cacgttcagc aaattcatag aaaggagcca atttcgttaa ctgttgatta
    16261 cattgatcac tatcatgaca aatataattc ccctttttcg tatacgtgcc atcagaaccg
    16321 gctttcgttg tagaaagaaa gagcgagaca ttcgatgttt tctgacagat cgcacagatt
    16381 cccttattaa tcgttggtga taaagtaccg ctgatccctt ttaattttcc atcttcgtaa
    16441 taaaccatga attttttttg cgtccctgga tcattccaac cgatgtacgt atgtgcttgc
    16501 aagtcgattt cattccaagc gggttgtttg agttttttca cttttcggaa taatttctcg
    16561 atttgtggtt tgctcggctg cataaacggc tggacgattt gcttcagctc ctccaatgct
    16621 cgttctgctt gcgtttttgt cagttttaca tctaaaactg tcgttaaaaa cgtatcgatt
    16681 tctgatggtt cgtaacctaa tacatcgcga atcttttcag ctgctagagc ctgaaccgtt
    16741 tgtaccgttt ttgagtcatt gacagatgag tacgcattta ataactgctc tacttctctt
    16801 tttataaaaa aatattgata tggtttcatt gtttgttcca tgattttaaa gtcctttctt
    16861 ctaaccaaag tgcatacttt gtagaaaaaa gtgatcttct gcaaagtatg tgttacctga
    16921 agatcccgga ctcttttaaa taagatacgt tttgcatctt atcatcaccc cctttaatgt
    16981 tcattcttct atgtacatga ttcattcgat aaataaaagt aaaccgtatc ttgagcagat
    17041 agtcaactgc aaacagaaaa aggactgtga caaaagtcgt gtcaaagtcc ctttctacaa
    17101 acaaacggtg ttccagaagt agcttcttca aaaaataagc cggaatttca caaaaatttg
    17161 agaagcaatt ttcgtaaatt ccttcttatt tcttgaagct gacccacttc tgtcacaacc
    17221 tcaatatggt ctattattgg ttttacctgt taaatcgagg attcttttcc gaagtaacgt
    17281 tgccatctct tacgcatact tgctgacata ggctgagcgg cttctttgac tgcgcagtat
    17341 ttgtctacga atgttacgat atatccttct ttgtacttag gcggtgcaat cgttgcgccc
    17401 cacatgtgct tcaaaatgat atctcgctca agatccgaca aatcggtgat cttttctgca
    17461 ttctttacag cgattcttgg atggatatac gcatgtgtgc cttcatcaaa tttcgtcgta
    17521 cgccaatcat agtagaacag atcatgcaaa agtccagcac gcgctgttgc tttagcattg
    17581 ccaccccatt tttttgcgag cagataacta tagtaagaaa cactgatgga atgctccaaa
    17641 cgagtggaat gaacatgctg tgtgtaattc gctaattttt tcactgcttc tgtttctaaa
    17701 agatcttcta cataagacat atattcttcg tcttcacgcc atttttctgt cataattatc
    17761 gatctgccac ctttttgaat ttcattttct tctgaaattg atctaagtat accaccagtg
    17821 ttcggattat tctagtcgcc ttggataaaa tagccaaagc ttaatagttc cttaatcata
    17881 aagaaaaatg ataaagaaca atccctgccg caaccccaac attcaacgat tccgcatttc
    17941 ctttcatcgg gatatatacg ttctgatcag tcaaagacaa gatttcctga cttaccccct
    18001 gaccttcgtt ccccatgatt attgcgaagt tatctgattt ttctactttg tctaaggcgt
    18061 gtgcttcttc atttaattct gtgccataca ctgtaaaccc tttgtctttc attaaaggaa
    18121 tcacttctgt caaatctttt tgcaacacag gcaaatgata attgcttccc tgcatgctgc
    18181 gcagcacctt tgtactataa atatctgccg ttcctttgcc taataaaaca ccagccatat
    18241 caaatgcatc tgccgtacgg atcatcgtcc ctacatttcc aggatcttgt acattatcta
    18301 ataacagcca tcctcctgaa aaatctggcg tgccttcagc cgttaacgcc atcactgcta
    18361 agattccttg tggtgtaggt agatccgata cagcatccat gacctcatcc gaaactagaa
    18421 aagtagaaat ctctttgttt gcggccagcc agtctcccca ttctttcatg cctcgctgtg
    18481 tcacaaaaag ttcttttatt tctgcatcag acgacgcagc ttcttcaatc agatggaacc
    18541 cttccaaaag atattcttcc ttttcttccc gatatttctt tttatgtagt ttttttaatt
    18601 ctttgattct tggatttttt gctgattgga tctctttcac attcctcacc tccccatgat
    18661 tatatcatgg aataggtgct tgagcttgtt tcatcctaaa aaaatcggta tcattaattt
    18721 ataaaatagg aaagaaaggt gacgaacatg cgcaaagtta aaatgaacgt ccaaggacgc
    18781 gtccaaggcg taggatttcg ctacatgacc aagatggtcg ctgatcaact aggcgtgact
    18841 ggaacggtaa aaaacgaaga tgacggctca gtcacgatcg aagcaatcgg caatgacgac
    18901 atcatccaga aatttattga agaagtcaag aaatctccga gcccaagtgg acgagtgcag
    18961 tatgtggatg tccaagaaga cccgatgatt gaggaacgaa agaaatttga tgtggtggga
    19021 taaggttgtg aaaaagctgg tctgcatcaa gcattaagga caggttgtga aaaatagctc
    19081 tccatatttt tcaacaggct ggacttaatg ccgtagatgc aagcttttga acaccgtcaa
    19141 taaggttgtg agaaagtcgc ttagttttga gtagtaagga gaattttgtg aaaatggctt
    19201 tccacatttt tgcataaaat ttgacttaat accgagaaac tgacttttga acaccgtcga
    19261 taaggttgtt tttgagacgc tttgcctcaa gtattaaaga taggtagtaa aaaagctgat
    19321 ctataacttg caataaattt gcttaaccca tattttccag acagaacaaa aaagttttta
    19381 gttttttaaa taagaaagaa acacttggaa tcactctttt ctttccaagt atttctttct
    19441 tgattttttg tctttttttc aaaatttttc gctacgtaaa agaacgaata tttatatgaa
    19501 atttctatga agttgattga attccatgcg cttttttagt atagttaagt ttgtgtaaaa
    19561 attatagaag aggaagtatt ctatgaataa atggaaaaac tggttattgg gatccggact
    19621 tgtcagtatc ctgctctttt tatctggctg tgtgcggatg gattcaaacg gaaaccctga
    19681 cacttcggga atcgtttatc gagtactcgt ccatcctatg ggccaagcga ttacatatct
    19741 agttgagaat ttcaattggt cttatggctg ggcagttatt gcaatgactg tgatcgtccg
    19801 tatcatcatc ttgccactag gtattagtca atcgaaaaaa acaatgatcc aatctgaaaa
    19861 aatgcaagcg ttaaaaccac aagttgaagc agcccaacaa aaattgaaag ctgcaacaac
    19921 acgtgaagaa caaatggcag cacaagcaga aatgcagcaa gtataccgcg aaaacggatt
    19981 gagtatgacc ggcggaatcg gctgtctgcc tctattgatc caaatgccga tcttctctgc
    20041 cttgtacttt acggcacgtt atacagaagg tattcgggaa tcatctttct acggcatcga
    20101 tttaggctca ccaagtatgg tacttgttgt cattgcaggg gtcgcctatc tgcttcaagg
    20161 atacatctca acgatcggta ttcctgaaga acagaaaaag acgatgcgta cgatgctaat
    20221 cgtctctcct gctatgatcg tcttcatgtc gatcagtgcg ccagccggtg ttacacttta
    20281 ctgggtagtc ggtggtgtgt tcagctgtct gcaaacattc atcacaaacg ttatgatgaa
    20341 accacgtttg aaagcacaag tggcagaaga gttgaaacaa aacccgccaa aacaagtcgt
    20401 cactccaaga aaagacgtga cagaatcagc aaagccagct aacaacaaac aagcaacaaa
    20461 acaaatcaaa caaccaaaaa acagtacaaa caacaaaggc cgcaacgcag gaaagcagca
    20521 gaagcggaaa taaaaacata ggacaaaaaa gatgagagct aagccctcat cttttttgtt
    20581 actcttcctc ttctgctatt ccattaatta attcgttgac agagacaatg ctttcacatc
    20641 ctgcacgaac aaactcgggc tctgaaccaa tcatttgtga gttgataaat tcaatgacca
    20701 tcggaagatt catcccagca taaagagtaa tctgttgtcc ttccattatt agtttagaaa
    20761 tgatatttgc aggtgtacca cctaaaagat cgcatagtac cacaaaatca tttaacgaat
    20821 ctgtgacagt gagaaacttt tgtttgtatg tttcagtgcc atcttctggt aataaaggta
    20881 acgtgtagat atcctcttga ggccccatga tcatttctgt actttttttc aattcctcac
    20941 aaaattttcc gtgactaatc aataccaatt gtctactcat ttttcgctct cccttatttc
    21001 gaaatcacac ccaatgcaga taaagcaaca cacacaatta atacgataaa aatcgctcga
    21061 gtaggagtca tgtttttctt tcctaataac caataaacac caccgacgat cccagcaggt
    21121 aaaattttag gtaagatcat atcgatatag ttttgcatat tcaatgtcac atcgccaatc
    21181 gttggtgccc atgaaaattt aacattgatc attgtcgcaa ctaatgcccc taccatgaat
    21241 acccctaata aagtggctgc gtccgttaat gcagttaagc gagattgcat agtagtaact
    21301 aaggaaattc cttctttgta tgcaaattca agttgcttcc aacgaaaaac catgacaacg
    21361 atctgagcaa aaatccatat aaacacacct atcggatttc catcagttgc catattcgcc
    21421 gccaaagccc caaaaatcgt tggaatcaaa gcagcaaata cggaatctcc aatcgctgca
    21481 aaggaaccca tcaatccagc tttcagcccc gatacggttt cctttgaagc aattccctct
    21541 ttttcttcca ttgcaagatc aatcccagta atgattgtat taaaaaaatt agaagtatta
    21601 aaaaattgcg tatgtgtttt catcatctct tttaattctg gtgtattgtc tccatatatt
    21661 tttcttagtt gtggcaagat agtatataaa tagccagaac cctgcattct ctcatagttc
    21721 caacctaatt gaaaagtgaa caaacttctt cgattgattt gcttaaaatc ttcttttgtt
    21781 aatttgtaat tagatttcgt catcttcgat ttccccctca acagaatgtg atggctcact
    21841 agctaatggt tgatggtctt tgttttgatc attcttataa ctgatcattg ctagtgctag
    21901 accaatcaat gctacagcaa gcatcggcag cgaattgaaa gttgccggaa gatctttaac
    21961 aacgcttgcc acacttgttc ctaataattg aacattagag aataacgttg ctaataatgc
    22021 cgtgatcgta aatcctaaaa taagataaga tagatgtttt tttactggta aatagcgcag
    22081 aagaatggca aacccaacag ctggtaaaac agctcctgct actgaaagtc catcgcctag
    22141 ccattttaga ttcccgttta aaacagaaac aacattttca actaatccac caccaaatgc
    22201 taaagctaaa aatacaggaa tcatacgaga gagcgaccaa ggtaaagctc ccattaagaa
    22261 attgcgttcg attgcttgat aattcatgtc ttctacgtga cggtcaatac gatgggcaaa
    22321 gtatgtgtta gcgaatctag ctaaaatatc caattggatc attaagcttg ctacaggcac
    22381 tgcaatagca gcaatcgctt gttctggatt cattccaaga gaaatcgaga aggcagttgc
    22441 taaaatcgtc ccagagttgg catctatttt tgatgcgcct ccaaacgtcc ccactcctaa
    22501 aactgtcaac tgcatactgc caccaataat caaacctgcg tttaaatcac ccattactaa
    22561 acctgcatat aaccctgcaa acactggcgc actcattgaa gaatagatct gtaattcatc
    22621 taaaatttga taacctgcat ataatgttaa taataagatc tgccaccatt gaatatccat
    22681 ggatcattcc tcctatttga ttaaaagatt cataaagtct tctgctttat cgtttggtac
    22741 catttgagca attaaatgta taccaagctg atctaatttt ttaaatactt cgatatctgc
    22801 atctaccaca ttgattgatt ttgtaatgga ccgtgtttca ttcgtttgtg acatattccc
    22861 cacatttact tctttgatag gaactccttt ttctactaaa gataagattc gatctggctt
    22921 ttttgctacg atcaatactc gttgagagtc atatttgcca gctaaaatat ttgttgctgc
    22981 tttttcgatt ggcaaaatgc ttaattttac ccctgctgga gtcgctaact tcaatccact
    23041 tttttcaatt gtgtttgtgg ctacctcatc atccacgacc atgattcgac taatattcag
    23101 tttcgttgtc caaagatttg ctacttatcc atgtatcaaa cgtccatcga ttcttacacc
    23161 aataattgcc atcatgcatt ctccttatct attttttcta tttattgttc aattaaaact
    23221 gtgacatcat caaatatccc ttctgtttca aagataacgt tttcattttt tctttcttat
    23281 atttcatttt taagctaaga acaaattcct tcccaactaa agaaaacgct ttcttttata
    23341 tttaagttca tttgattttc ttaacttgaa tgtgtttcat caacatcgta actttcatag
    23401 ggtcgatcca gccagttttc tcttgttgcg cgtttgccat accacaagcc atagaaaatg
    23461 cgagaatctc ttcgattgat tgcctttttg ataacgcatg ggccgatcct gcaagggtcg
    23521 cgtcacctga accaacagga ttaacaactg atattttagg aataatgact tcgtaaaaac
    23581 actgagcaac ttttgccaca gcacctctct ctcctaaaga aacaacaacc catggcactc
    23641 cttcaaatat tggatgagct aataaggact gaattttttc taattgaatc gaagaacaga
    23701 tacgttggcc aatcagttct cctaattctt ccagattggg cttgataagt agtggcttct
    23761 ccttaccttc caatacttca agcaaaaatt taccagaagt atctagtaat atgtctttcc
    23821 cttttttatc agctaaagca accaattgct tatagtaatc aagagggcat ccttggggta
    23881 aactacctga gatcgtcata atatctgata tttcgaggag ctgctcgaaa tgatcggtaa
    23941 atgtctgttg ttctttcttt gttatgaagg ggcctcgttc caaaagctct gtttgtctcg
    24001 aactatgcag gatagcaata gaatcccttg tctctccttt aattggaaca aactgatgca
    24061 tgatccctaa ctgatctagc tggttcttta gatattcacc gtgctggcca cctattaaac
    24121 cagtagctat cacctcttct cctaataaat ttagaaccct cgccacattc actccttttc
    24181 ctcccgctgt tttattgact gtttcaactc gattgacttt gtcgaggttt aattcttcaa
    24241 ttgtatacgc gatatcgatg gaaggattca aggtaactgt cgtaatcatt tcatcttcac
    24301 ctttttgctt tttgttttat tgaaccaagg ggtagcggtt tcatttatta ctttatttaa
    24361 agtctcgatg ttttctctcc catttgttct taaccaatga atagctccgt cttttccatt
    24421 ttcgacgtat gctttgaccc catctttcca tgtcgcacgt ccacacaata caccattgaa
    24481 ggtggaaccc gattcatgag caaaaatcag tgtttgttga aataattctg ttgaaacacc
    24541 cccactcaaa aaaatgaatg gtaatggagt cgcttcactt tgctccttaa agaaggcagc
    24601 agcctcttct ttgctgtgaa taaattcacc tgtggaatag ccattcacat aattcatatt
    24661 gactggtact tctactttca acacatcaac tttatatttt ggatcagaaa attctttcat
    24721 catctcaata actttgtggg gcttgacttt cgcatactct ggagaagaac tatcgcttat
    24781 ttgtgcatca taagaaacta actccaaata gaagggacga ttttctcctt cgcactcact
    24841 acctaaacgt tcgatgaaca catgcttcaa atgattgatt tcgggtgctt catcaacatc
    24901 gtagtaaagt aagaatttta ctgcatccgc ttgctgttct ttcaatctga gagaagacca
    24961 atccgctaat aaatcaggta gacgaccagg cgtagttgcg tcatagccgg ttttctcata
    25021 agctaacaat aaccccgtct cttttgctct gacttttgct gctggcaatc catattccgg
    25081 atccaataaa atagcagaag catagggtgt cagctcagct gatactagtt ctttgaattc
    25141 actgatttgg tcttcacttg gctcttttcc caacgatttg atcatttttt ttagagcacc
    25201 acgctgatcg atcgctaatg cactaataat cccatctttt gttgatagtc tctccattgc
    25261 cctatttttt tcttcattca tctgtcattt ctccttcggt ttcatactta tgaataatga
    25321 cgcccttgac tactcgattg accgtgcctg ttggagatgg tgtatctggt ttattagcta
    25381 ctttgattga agtatagagt gcaatgattt gtgcagtcat aatcatcgct aatgcaagat
    25441 aggcatcggg caatttactc accgagtctt ccaatagcac agtcatacct gagaaatcaa
    25501 tagtacccgg ttgcgcaatc gcaatgacat tggcagtgac ccgatcttct tttatttcct
    25561 ctaaaatatc taagtcataa tttcgcgtat atggttgatt gctcacaaaa cctacaacga
    25621 gtgtctcgct atttaaaaaa gactttggtc catgacgaaa ccccatcgaa gaatcaaaaa
    25681 ttgtagcaac tttacctgct gttaattcta aaatttttaa ttgtgcttct cttgtcgcac
    25741 ctgccaaact tcctgaaccc agataagcga tccgttcaaa aaaatcaggt aataactttt
    25801 gaatttcttg atctcttgaa agaagatctt gaccgatttt aactaattgt tcaagatatc
    25861 tttctttttt ctccattgct gtttgatcaa agatccataa agcactcaat aacatacacg
    25921 taaaacttcc tgtcatagca aaacctgcat cgtttgctct ctctggcatc aataacagat
    25981 aattctgttt atcatcctgt gcgttttttg ccaacatccc ctctttagca caagtaatcg
    26041 tcaaatgaaa caatgtttca acttgttcat ttgcaatttg cacagccgct acactttctg
    26101 gactgttgcc actgcgagca aatgaaacta ataaagtcgg ttcatttgga aacaaaaatt
    26161 cttttggtga agctacaata tctgtcgtgc caaaactgtg gaagataaaa aactgattgt
    26221 ctccatgttc atttagatag ggtaagagcg tatccccaat atattgagac gttcctgcac
    26281 ccgtaaaaat aacacgtatt cttcttccgt ttgcttcttt ttttatttga tcaaaaaata
    26341 gagctaaatt ttcttggctt tgcatcaatc tatcgattgt ttctttccag agatctggtt
    26401 gttgttttat ttcttgtgtc gtgattgctg cacctatttg ttttaatttg ttcttcgata
    26461 gctcaaacat gttgttctcc ctctcaatca ttttcggata tgccgaattt tataatgaaa
    26521 ctggtccgct cttgctacac tatctgtata ttcgataatc tcatttttca cgttataggt
    26581 tatcctcgtc agatgtaaga cgggagcgcc atcagcgatt cgtagcacaa cagcatcttt
    26641 tttttgcacg atacttgcat aaaactcttc atcagctaat tgaacttgtt gttcataatc
    26701 ctgtgaaaac acatcataca acggtttact ttcaagtaac ggcaaatcaa gtcccataaa
    26761 tagtttcgcc ggtagataag aacgctcaac catcaatggc tcttcatctg ccaaacgcaa
    26821 tcttttaagt ttaaatacag tttcacctaa atggatgttc aaattcttag caattctctt
    26881 attagcttcc atttgtctaa attctaaaat gaccgtctta gggacgcgcc ctatttcgcg
    26941 catttcttct gtaaagctat atgctccagc taagttcgat gacttatcag acaaatcaga
    27001 gacataggta ccttttccat gtcttcggta aatataacct tgattttcca attcctgtaa
    27061 agccagacgt acggtggtac gactcacccc atagtgttga caaagttctc tttcagataa
    27121 cagcttatca tgcggagtca tctgtgtttc gatacgatcc ttcaataaat caactagttg
    27181 ataatacaat gattttcctt tatttgagct ctccataaaa atcacctctt ctttactggt
    27241 tataaccaca acacaagaat agtgaattat taatcatttg tcaaaggttt tttagaccaa
    27301 aaaagctatt ataacagctc tttaaagctt tatatcttga atagacaaag atacctcaaa
    27361 aataccagtt accaataaaa gaacagaaaa aaagacgaac cacataagcg atccgtcaaa
    27421 atggtctaac ttatcgagca gcgtgatcca cattaatccc tatgatattc ctacaactat
    27481 cgttttatta cttccaatat catttacaac aactaaaaat agcttattaa tgacaaatgt
    27541 tattattagt tttagataac ttttgcagtt ttatactttt tttctataaa atttgacaaa
    27601 cattttttta aataactaat tattttttga atctgtcact cagacaatcc tttctcttac
    27661 ttgttaacat aaatagaagt tgctgtttct tctaatagtg tacttaattc tttaatattt
    27721 tgttttcctt cttctaacat ccaactactc gctttttctt cgccttcggt aacaaaaggt
    27781 gctactccct tcgcccatgt tgctcgccca cataagacac cattgaatgt cgaacctgat
    27841 tgttttgcaa aacgcaatgt atcttgaaat agtgcagcgc tgactcccgc acttaaaaaa
    27901 ataaacggtt gttcagtcac tttcgattgt tcatagaaat acgcggatgc ctctgcttga
    27961 ctataacaag tatcttgccc atatccttcc acaaatctca tattcactgg tacttctact
    28021 ttcaatacat ccacttgata acgttctttt gaaaattctc gcatggcttc aatcacttta
    28081 tgcggtttta cttttgcata gtcaagagaa gacggatcgc caatagttgc atcgtaagaa
    28141 aggagctcta aaaagaaagg tatgccttct gcttcgcatt ctgaaccaat ccgttctata
    28201 actatttgtt tttgcacatt aatctctact ggttcatcaa cgtcatagta caataaaaat
    28261 ttacaagcat ccgcacccac ttcttttaac cttttcactg accaactatt cagtaaatct
    28321 ggtagtcggc ctggcatttt cgcatcatag ccggtttttt cataagctag caacaaacca
    28381 cattctttct ctcgaagcgc tgctgcaggc agaccatatt ctggatcgag taaaatagcg
    28441 gaagcatacg gagtcaggta ctgagaaacc atctgtttaa attggatgat atcttctgat
    28501 gtagcttctt tatattggtt gatcattttc ttcaaagcac cacgttggtc gatagctagc
    28561 gcgctaataa ttccagcttc atccgtcaat cgcttcaaac gttcctgttt ttctatactc
    28621 attttcttca tatatcttca cctatttctt tagttaatat tgtagctatt tctatttttg
    28681 attttgcagc tctcaactgt tctgtaacct cttttcgcat caatgatcgt gcaacgagag
    28741 acaataattt caaatgagtc gttcctgctt ctgaatctgg gataaataac gcaaaaataa
    28801 aatcaactga ctgtctatct aaactgttcc attcaattcc ttttttcagt gtgacgataa
    28861 tcaaactagg ttcattgatt gtttgagact tcgcatgagg gatagcaaaa cctgacatta
    28921 ttcctgtagt accttctttt tctctttcca gaagtttttg atatacttct ttttcgtcat
    28981 gagcgattcc taaacagaat gtttgctttg ctaaaaactt aaatgcttct tcttgattat
    29041 ctaatggctg ttgtaaaaaa atgttctctt catgaatgat tgtcatgata aacctcctgc
    29101 tcttccttct tcgataactg tacgatttgt tgctttatta tccctcttct tgtctttatc
    29161 agttactctt ttcttttgga tcaaacctag tatcactcca ccaacgatcg aaccaataag
    29221 aatcgctgta acccattgga tttttcccgt cactacaggt aacactaaaa aaccgccatg
    29281 tggggctggt acttggacac caaacagata ggtcaagaca gcagcaattg atgagcctaa
    29341 catcatcgtt ggtaatactc ggatcgggtc tttcgcagca aacggaatag ctccttccgt
    29401 aatatgagtt gatcctaaaa taaaattaac caaacctgca ttacgatcat tagtatcaaa
    29461 atatttacca aacaataaag tagcaaaacc agtaatcaac ggtggtgcaa tgcaagctgc
    29521 tgacgcacca gccataaaag tcgtgtttcc ttgtgtcaaa agagctgttc ctgttacata
    29581 tgccgcctta ttaaccggtc ctcccatatc aaacgcacac atacagccaa caatcacgcc
    29641 aagaacaagt ggattcgaat attcaaatcc agctaaaaag ctcatcattc cttcatttat
    29701 agccttcatt ggacctgcca gcaaagacat cacgatgcca ataaatgcta caccaataac
    29761 tggatataag acgattgcct tcaagccatc tagttgttta ggtaaaatta tcagtagctt
    29821 ttgcaaaaat aagataaaat aacctgctaa gaacccagct aaaatacctc ctaaaaatcc
    29881 agtaccgcca ttcatggcta cgattccacc aacaaatcca acgactaacc ctggacgttt
    29941 cgcgatccct tctgcaatat aagctgctaa aactggaacc atcattccca ttgctgcatc
    30001 gtcaactgct tttaccattg ccgctgtagc attgtattgt gcatgatctg gatcagaaga
    30061 atagattccc caaacggcaa atgaaatagc aatcaatact ccgccagcta ccacgaaagg
    30121 caacatatga gagacaccgt tcatcaaatt tttatagatg ctacgaccaa aattagcacc
    30181 acttatcgct tctggttcta aagtatcagc agcttcatta tctgctaaga cagtaccttt
    30241 tcctgccaat gcttcgtcaa tcagccgttg tgcatccttg attcctgtac tgacagaaac
    30301 atcaatgact cgttttccca caaatctttc tgaatggaca tctttgtctg cggcaataat
    30361 gacagcatct gcctgtttga tttcttcagc cgttaattca ttctcgatcc caatttgtcc
    30421 atgggtctcc actttgatcg tgatgccttt tttcctagct gcttgctcta atgcctcttg
    30481 cgccatgtag gtgtgcgcaa ttcctgtcgg acaaccggtt gctgcaatta gttgatattt
    30541 tcccatcttt cgaacctctc ttccttgatt ttgatttgtt tacttaattc ctccacatcc
    30601 aaaaaatcag tcagtccttt acgaaaagca gtagagctgc ctgctgctat gcttttacgt
    30661 aacgtttcat caagcgatcg tcgattcatg tatcctgcta aaaaagtgcc taacatggca
    30721 tcacctgcac atgctgtatt gacaactttt ccttttggcg agtttcctcg aatcaccctg
    30781 tctttcgtaa aaagaatcgc tcctttctca ccgagggata acaaaacatt ttctgcgcct
    30841 gactctacta gtcgttgtcc ataaacaaag taatcttcat ctgtaaccat ttttcgtccg
    30901 aaccaagaag aaagttcttc ctcatttggt ttcagcaaaa acggtcgata gggtaaacaa
    30961 tccatgactt cttgatcact actatcaata atcaaacaaa tccgactgat ttctattaaa
    31021 acctttggag acagccctct tggtaaactc ccagaaacac aaagataatc ccctgcttgt
    31081 aatgctctta tttgatttaa aaaattatga acagctgtct gcggtatttc aggtccttga
    31141 ttcactaatt tgtattcttc tcccgtttga ttgacttgtg taaatacatt gatacgcgtc
    31201 ataccaggca cttcaataaa ctctgtttgg atttgtttct gctgtaaata gtcttctata
    31261 tactttcctg tgaacccagc agaaaaacct aatgccgtac ttgggaaatc tagcatcttt
    31321 aatactagtg acacattcac cccttttccg tttgcctgaa tgtcatcctc ttttgtgcgg
    31381 tttaccataa aaggatgcat ctcatctgtt tcaataaata gatcaattgc caaattcatc
    31441 gtgcatgtgt agatagacat aaccaacgac ctccttgatt ctattatgaa atacgcgaaa
    31501 tagaaaatgt tttcttaatc gtcccaacac aaagaaaacc tttacattta taaactcgtc
    31561 gtgctttttt taactaactt ttggtgaaag aaggaaagaa tatgaatcac aaattaagtg
    31621 aaacagagca gtacctctgg aactttatcg aacacctcat cctagagatc cctaactatt
    31681 ccatcgtgaa actaagtgaa caagcaaatg tctcaacagc caccattgtg cggacaatga
    31741 aaaagaaagg ctatgaaggg tttacttcgt ttaagcatca tctaaaagaa ggagaaaaca
    31801 agaacatcaa tttttcattt ttagataaag tagacaaagg aatccgccga gccattctaa
    31861 aaaatgaaca agaagtggta cgtacaatca atatgttgga aatcgggaac attgaagatg
    31921 caatacaaag aatcaaattt gctgaccggg tttttgtctt tgcccgagga ttttcagaaa
    31981 tgattggcca agaaatgctg gtaaaacttc aattaacggg aaaaaattgt cagatgcata
    32041 ctgatccaga aattatcaaa agcatcagtc ggaaactaac taagaaagac agcgttattt
    32101 ttgtttcttt gaacggtgag aaaacaaata aaaacatcaa aaataagatt tatgtcttta
    32161 tttttcaatt ttttaaat
//
LOCUS       DARBEC010000024        31988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.024, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000024 DARBEC010000000
VERSION     DARBEC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.024"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..273)
                     /locus_tag="SCL80_001051"
     CDS             complement(<1..273)
                     /locus_tag="SCL80_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1106936.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPGPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGING"
     gene            complement(275..472)
                     /locus_tag="SCL80_001052"
     CDS             complement(275..472)
                     /locus_tag="SCL80_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEG1106937.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(866..2593)
                     /locus_tag="SCL80_001053"
     CDS             complement(866..2593)
                     /locus_tag="SCL80_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEG1106938.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2586..3779)
                     /locus_tag="SCL80_001054"
     CDS             complement(2586..3779)
                     /locus_tag="SCL80_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEG1106939.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(3966..4610)
                     /locus_tag="SCL80_001055"
     CDS             complement(3966..4610)
                     /locus_tag="SCL80_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1106940.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4704..4838)
                     /locus_tag="SCL80_001056"
     CDS             complement(4704..4838)
                     /locus_tag="SCL80_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEG1106941.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="SCL80_001057"
     CDS             complement(4840..6972)
                     /gene="feoB"
                     /locus_tag="SCL80_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEG1106942.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(6969..7442)
                     /locus_tag="SCL80_001058"
     CDS             complement(6969..7442)
                     /locus_tag="SCL80_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEG1106943.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            7843..8067
                     /gene="nrdH"
                     /locus_tag="SCL80_001059"
     CDS             7843..8067
                     /gene="nrdH"
                     /locus_tag="SCL80_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEG1106944.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8226..10385
                     /gene="nrdE"
                     /locus_tag="SCL80_001060"
     CDS             8226..10385
                     /gene="nrdE"
                     /locus_tag="SCL80_001060"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEG1106945.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFLSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYAMAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10435..11400
                     /gene="nrdF"
                     /locus_tag="SCL80_001061"
     CDS             10435..11400
                     /gene="nrdF"
                     /locus_tag="SCL80_001061"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEG1106946.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11489..12628)
                     /locus_tag="SCL80_001062"
     CDS             complement(11489..12628)
                     /locus_tag="SCL80_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1106947.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAV
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(12937..13650)
                     /locus_tag="SCL80_001063"
     CDS             complement(12937..13650)
                     /locus_tag="SCL80_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEG1106948.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="SCL80_001064"
     CDS             complement(13904..14605)
                     /gene="nagB"
                     /locus_tag="SCL80_001064"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEG1106949.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            14839..16371
                     /locus_tag="SCL80_001065"
     CDS             14839..16371
                     /locus_tag="SCL80_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1106950.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16562..17764)
                     /locus_tag="SCL80_001066"
     CDS             complement(16562..17764)
                     /locus_tag="SCL80_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1106951.1"
                     /translation="MKSNEKKPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMAALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTVFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQTVVFKG
                     KRNLENKK"
     gene            complement(17846..18535)
                     /locus_tag="SCL80_001067"
     CDS             complement(17846..18535)
                     /locus_tag="SCL80_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1106952.1"
                     /translation="MKKKIIRLILISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(18641..19009)
                     /gene="rplL"
                     /locus_tag="SCL80_001068"
     CDS             complement(18641..19009)
                     /gene="rplL"
                     /locus_tag="SCL80_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEG1106953.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(19062..19565)
                     /locus_tag="SCL80_001069"
     CDS             complement(19062..19565)
                     /locus_tag="SCL80_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEG1106954.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(19769..19896)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(19975..20664)
                     /gene="rplA"
                     /locus_tag="SCL80_001070"
     CDS             complement(19975..20664)
                     /gene="rplA"
                     /locus_tag="SCL80_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEG1106955.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(20768..21190)
                     /gene="rplK"
                     /locus_tag="SCL80_001071"
     CDS             complement(20768..21190)
                     /gene="rplK"
                     /locus_tag="SCL80_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEG1106956.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            21533..22201
                     /gene="sdaAB"
                     /locus_tag="SCL80_001072"
     CDS             21533..22201
                     /gene="sdaAB"
                     /locus_tag="SCL80_001072"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEG1106957.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            22214..23086
                     /gene="sdaAA"
                     /locus_tag="SCL80_001073"
     CDS             22214..23086
                     /gene="sdaAA"
                     /locus_tag="SCL80_001073"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEG1106958.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(23177..24196)
                     /locus_tag="SCL80_001074"
     CDS             complement(23177..24196)
                     /locus_tag="SCL80_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEG1106959.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(24369..25274)
                     /locus_tag="SCL80_001075"
     CDS             complement(24369..25274)
                     /locus_tag="SCL80_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1106960.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(25333..25878)
                     /gene="nusG"
                     /locus_tag="SCL80_001076"
     CDS             complement(25333..25878)
                     /gene="nusG"
                     /locus_tag="SCL80_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEG1106961.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(25979..26149)
                     /gene="secE"
                     /locus_tag="SCL80_001077"
     CDS             complement(25979..26149)
                     /gene="secE"
                     /locus_tag="SCL80_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEG1106962.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(26173..26325)
                     /gene="rpmG"
                     /locus_tag="SCL80_001078"
     CDS             complement(26173..26325)
                     /gene="rpmG"
                     /locus_tag="SCL80_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1106963.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            26487..27131
                     /locus_tag="SCL80_001079"
     CDS             26487..27131
                     /locus_tag="SCL80_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1106964.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(27187..27690)
                     /locus_tag="SCL80_001080"
     CDS             complement(27187..27690)
                     /locus_tag="SCL80_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1106965.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(27703..27749)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            28287..29252
                     /locus_tag="SCL80_001081"
     CDS             28287..29252
                     /locus_tag="SCL80_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEG1106966.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGDDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(29351..30523)
                     /locus_tag="SCL80_001082"
     CDS             complement(29351..30523)
                     /locus_tag="SCL80_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1106967.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMPTIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(30510..30635)
                     /locus_tag="SCL80_001083"
                     /pseudo
     CDS             complement(30510..30635)
                     /locus_tag="SCL80_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            30743..31822
                     /locus_tag="SCL80_001084"
     CDS             30743..31822
                     /locus_tag="SCL80_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1106968.1"
                     /translation="MTQTHDNTVSRKGKHLSYSERCQIAILKKEGYSNRQVAKVLGRV
                     PQTINNEIDRGTITQLKRQTQNGKVYDTYYSVYDADAGQAFYEKQRLNCGRRPKWANT
                     NAFIDWADDKMLNEKWSPDVVVGFARKHELFDCAIIPGTTALYQWIDRGIMRTKNMDL
                     LEKLSRKIKAVRPGHRPNKRVLGTSIEERPKEVETRETFGHWEIDTVVGNKTKSDAVL
                     LTLAERQTRFEVILKLNGKDTESVDQAVQQLRERAGANFDQLFKTVTSDNGSEFSGLH
                     DALQDVADVYFSHPYASWERGTSENQHKLIRRFVPKGSPISEVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFKQERLVA"
BASE COUNT         9759 a         6606 c         5737 g         9886 t
ORIGIN      
        1 accatttatt ccagcagttc ttccaatttc actcaaattt cttattaatt cctcttgata
       61 ttcagtgaat cttattttat tagtataagc agtaaaatat agataattca ccaatctatt
      121 tattaaagga ccgggatcta attcatattt ttgtttttct aattttaaat ataccttttg
      181 taaaacctca agaatatcag aatatttagg atctttactt atagatatat cattatataa
      241 ttcatgaaca atttgcttag catttttttt cattttagca cttccctgga attgctccac
      301 ctaaaaaacc acctatagac attcctgcaa tacaagtagt tgccttggcc caatcgaccg
      361 tacatttatt tttagtgcaa tacactccat ttccataata ttttccacta tgagtggtac
      421 ccccatagat aagttgtgtc tctttaatag acaaaatttt taaatgtttc ataatctaat
      481 cacccctaaa ttaatgtttt tagactatta ttctaacatt cattaaattt caaaaaacaa
      541 ttccttcttg aaacgtagca aaataattcc tgaataagaa tttaatcact tttaagtata
      601 taaactatgt atattcttta atattttttg agaatgcact tcgtacataa taaactttaa
      661 tcacattagt ctttcatacc tacgatcgat ctctttgact cgcacccata aattcataat
      721 agtttgtcgg cgtctttgga cgtttcgaag atgagctacg cattctcttt ttttatagac
      781 aaaaaagata gagacgaaca tggagcttcc aaatttggat atttgatcca attttggaag
      841 ctccatttat ttcgtgtctg tctttttatt ttttccagct ttcgactaat tcttggttct
      901 ctttgatcca gtcttttgct gcttgctgtg gatcttttcc attgttgatt tccaacataa
      961 ctttttccat atctttttct gaccaatgga aattgtctaa tactttgtaa gcttctggct
     1021 ggtcttcttt caatcctttt cgaaccatcg tatggatctg ctcttcgctt cccattccgt
     1081 tttcaggatc ttctaagtat ttcagatcat atttggcaaa catccaatga ggtgtccatc
     1141 ctgtaactac aattggttcg tgttttttga tcgcttgacc tagtgcaacg gtcattgctc
     1201 ctgaagaaga tgtctctact ttccagtctt tcaagttgtc atatttttgg atcgtgtttt
     1261 cagcagccgt aactacacca gctcctggct cgattcctat gattttctta cctgcctgat
     1321 cggttaactc atcgatcgaa tttacatcca tgtaggctgg cactgctaaa ccaacttttg
     1381 ctcctgtcaa attggcacct aggtcttcga cttgatcttt atattgtgca tattgtgaac
     1441 catgagtttt aggtagccaa gctgaaacca tggcatctga ctcgccattt gaaacagatt
     1501 tccacatgat tgaattatct aatggcgtca tcgttacatc atagcccatt tgtttcaata
     1561 cttcaccgac tacatttgtt gaagcaactt ctgtatccca ttcaacataa gataagttga
     1621 tacgttttgt ttctttggca gatgaaaaac tccctatacc aatcgcacct aagatcacta
     1681 ctactacagc accaatgatc agtccttgac gttttttact tttctgtgtt gttgctcctg
     1741 cagctttttt attgtttggc tggttcaaat gctgtgtaaa acggtcaatg atgattgcta
     1801 agataactaa tgcaaccccg ttgacaaatc cattcccgac ttgtgctctt tgcaatgctg
     1861 ataatacgcc gcgtcctaag ccgggtgcac cgatcataga ggcaataacg accattgaaa
     1921 gtgacagcat tgttgtttga ttgatccctg ccatgatcgt acttttagct aaaggcaatt
     1981 ctagtttgaa tagtttttga cgacttgtgc tcccgaatga atcagaagct tctactaatt
     2041 ctttaggaac ttgtcgaatc cctaaattcg taaaacgaac ggttggtggc agagcaaaga
     2101 tgactgaggc aaatacccct ggaaccatcc cgataccgaa aaatgcaact gctggaatca
     2161 aataaacgaa tcctggcatt gtctgcataa agtcaaggat cggtttgata atactttggg
     2221 ctttactgct ttttgccatc aagatcccta atggtacccc aatgatgatc gagatcacac
     2281 tagataaaag aacaagtgtg acagtattca tcaaatcatt ccatagacct tggttataaa
     2341 taaaccatag accgattaat gtgaacaacg taagtcctgg acgtttctta gaaataaaga
     2401 aagctgctac cgtcaataac aagataaaca aaggagctgg aatcactaac agtaaatttg
     2461 taatccccga catcactgcc tgtccaatcg tttgtaaaaa tgaaaatagg ccagaaaaag
     2521 ttgtcgtaaa ccattctgtg atattttcta cccagtttgc cactggtaat tgatagttca
     2581 atagattatt catgttcact cacctcgctt gtttctgcta atgcttcaat cacactacct
     2641 cggatgacga caccttttaa ttttccatcc tctacaactg caagtggagt cggtgagtca
     2701 taaataatat tgaaaatatc acttaccacc atgtctttcc caatcaccgt cacattttca
     2761 tctaggaagt cgatcaatgg acgtttttgc ttcctagctt ctaacgcttg atctgcagtg
     2821 atgatccctt tcaggtgtct ctttctgtca acagccatca gcatgctgac ttcttcatta
     2881 cgcatccttg tcaatgcaac gtttggtcca tcagattcga tattcgtcgt caatgcaggt
     2941 accatgatgt tttgagccgt aagcactttg gaacgatcga cttcttccac aaattcacgt
     3001 acataatcat ttgctggatt ggtcaatatt tcttcacctg ttccgatctg catgatttcg
     3061 ccgtctttca tcaaggcgat tctgtcccca atccgtaaag cttcattcaa atcatgggta
     3121 atgaaaataa tcgttttttg aacattggct tgcaggtcca gtaattcgtc ttgcatttct
     3181 cgccggatca gcggatcaag agccgagaac gcttcgtcca tcagcaagat ctcaggatca
     3241 tttgctaaag ctctggctag gccaacccgt tgttgcatcc caccagataa ctgactagga
     3301 tattggtctt taaatgaaag caaactagaa ttttctaatg ctttttctgc tttttcttgt
     3361 cgttcttctt taggtacacc acggacttct aacccgtatt ctgtattttc caagatcgtt
     3421 cgatgtggaa acagaccgaa gttttggaaa accatattga ttttatgacg acggacttct
     3481 cgcaattctt ctttgcttaa cttcgataca tcttctccat caatatagat actgcccgaa
     3541 gttggctcaa tcagacgatt caatagacgg atcaatgtgg atttcccact cccggaaagc
     3601 cccatgatca cgaagatttc tccttctttt acgtcaaaat tcacgtcata cacaccaacc
     3661 gttgcaccgg ttttctttaa aatctccgtt ttcggctggt gatcattcat catatctaaa
     3721 gcggcttgtg tctttttacc aaatatcttt gttaaatgtt ttacttctac ttttaccaaa
     3781 caaattctcc ttttttatgt aaattcattt ttctttctga aaaaagtgac cacactatgt
     3841 taacatcatg tagtcacttt gtcaaaaatg aaagaaattt gcttgtccaa actgaaaaaa
     3901 acttggctaa atctttgtaa ataaaaggtt tagccaagtt aatatttttt taatatttca
     3961 cacaatcatt ctgcaagctg atgctccggg taaaaaaagt ttgtcatacg ctgtagtgta
     4021 tattcatttc caataaaatg aacgatgtcc cctgccgtaa atttagcata ggggccaggt
     4081 gaaacaagca gttcttcatt atgagaaatt gcgataactg tggcgccagt cgcctgccag
     4141 atattcaact cgctaattga acggtccaga tgcattgctt tttctgataa agataattca
     4201 aacggaagta aaggattcat cttatgtatt cgcttcgttt gattgaccag ctgatccaat
     4261 aattgagaaa aatgattcag ctcatcttgt tgcttctcca cacttgccat gatttctgct
     4321 tttgtatctt ggatggattg tacatcttga tattgcaata ggaaatcttt tgctttttcc
     4381 ttcgaagcaa caaatgcccc acttccatgc cgtacttcca tgattccaag atctaccagc
     4441 acattgatag ccttgcgggt agtctcagga gaagcattgt acgtactggc aagtgttgag
     4501 cgggcatgga tcttctcacc cactttatat cggttttcaa cgatacgttc ggctaaatca
     4561 acagcaatct gctggtagcg cgggcggacg atttttttct ttccagacat tttctttcct
     4621 ctttcttttt ttactttccc tcatcttact atgctctagc aaaataagca aaaaagggga
     4681 gaatagcctc ttgcaacgac tgatcatcgc ttgatctgtt ccttttttac tgggcaagct
     4741 gtttgacagt catcacaatt cttaccggat tttaaacgag tatagacaat tgcgcctgca
     4801 cttccaaaga ttaacacact taataagaat gtcgccatcc tattcctctc cttctaatga
     4861 caatatagta taaaactggt tcttggattt tggctgacgg aacaaaacat aaacgagtcc
     4921 tatcatcagt aaaatagcaa gatacgtccc taacgctacc tgtccaccct ccaataagtg
     4981 tcccaactga taaatcacaa aactaactaa ataagctaaa ccgcattgat agcctatggc
     5041 gatccaagtc catttcttgg cattcatttc ccgatggata gccccaattg ccgcaaaaca
     5101 aggcgcacaa agtaaattga aggctaacaa tgaataagct gctgcgggag tatatgtgtg
     5161 ttgtagcact tgccagattt ctctgccatt ttcggatact tccgcatgac caaacaaaat
     5221 accaaatgtg ccaataatat tttctttagc gaccaatcct gtaatcgctg cgactgttcc
     5281 ttgccagttt ccccaaccaa gcggctgaaa aacaactgcc aggattcgcc cgaaaaaggc
     5341 taagatactt tggtcttcag aaacaggttg agcataaaaa ttataagaag agctgaacca
     5401 gatcacgata ctagaaacaa agataatcgt tcctgctttt ttaacaaaag acttgctgcg
     5461 gtcataggtt tgtcttagga ccgctgacca ttgaggaaaa tgatatgcgg gcaattccat
     5521 gataaaagga gctgtatctc cagcaaacaa acgtgttttt ttcaagctga ttccagagaa
     5581 aataattgca gtcataccaa gaaaataggc agaaggagct acccaagatt gatgaggaaa
     5641 aaaggcccct gctactaagg caataatcgg caactttgca gaacaaggca tgaaggttgt
     5701 gaccatgatc gtcaaacgac gatcttgctc gttttcaatc gtgcgactgg ccatgacgcc
     5761 tggcactcca catcctgtag caatcagcat aggaatgaag gattttcctg aaagaccgaa
     5821 tttcctgaaa agtcgatcca tgacaaaagc gatcctcgcc atatatccgc agtcttccag
     5881 aaagctcaag cataaaaaca atataatcaa ttgcggtaga aatcctaaca ctgctcccac
     5941 gcctgcgatg atgccgttca agatcagctc ctgcatccat cccgctactt gccattcagc
     6001 taacaatctg ccaacatgtt ctggtaacca tgtaccaaac aatgtgtcat tgatccaatc
     6061 tgtccccatt gttcctactg tttgaatagc cagataatag atcagccaca tcacaaatgc
     6121 gaagatcggc aaggctaacc aacgattcgt tgcaatctga tcgattttgt cactcatcga
     6181 taattggaaa gaatcatttg acacaacaca gagtgtacat agttgggtaa taaattcata
     6241 acgttcattg accagaatcg tctcactatc gtcgtccaat agtttctctg ttaactggat
     6301 cagttgctcg atctcttttt gctgagaaac tgctatctcg taatcctctc ttaccttctg
     6361 atctcgttca aaatatttca ttgcatacca gcgtttcttg ccgctttgtt ctcctagtac
     6421 atcttcaatt tcatgtaaag ctgcttctaa acgtggatca tatgtacagg gaacaggaat
     6481 agaaggccgc ttttttgcag actcctgaat cttttcgatt agtttttcga ttccttgctt
     6541 tttcaatacg ctgattggta aaactggtac accaagacca taggaaagct tttctagatt
     6601 gatttttttt ccttcttttt tgatgacatc catcatattc agaccaatga tcataggaat
     6661 gtctaaatcc aataactgcg tagttaaata aagatttcgt tctaaattgg tagcatcaat
     6721 aatgttgatg atcagttcag gctgttcctg aaccaaataa tctcttgcaa tcacttcttc
     6781 tggtgtataa ggagacaaag aatagatgcc gggtaggtcc tgaatatgaa tgttcggatt
     6841 ttttttcaac gatccttctt ttttttctac tgttacgccc ggccaattcc ctacatattg
     6901 attcgaacca gtcaagagat tgaacaagct agttttcccg ctgtttggat ttccgactaa
     6961 cgcgatattc attctttttc ctccctttgt atcaagatta gttcggcctc tgactttcgt
     7021 aacgtcagtt cataccctcg cactgtcagt tcgattggat ctcccagagg ggctagtttt
     7081 cggataacta tttttacatt tcgcgtcaat cccatatcca ttaaccgtcg cttcactgcg
     7141 ccttgtccgt ggattgctac gacgatccca gtctctcctg cttttaattg atcaagagaa
     7201 gtccagcttt cagaggaagc agtcttttta ttgacaaaga tttgacctag tatttcccga
     7261 cttactgcca ctcgatgact ttttaccagt aaaatggcat tgttgcctga taaattgacg
     7321 acgacgattt tacttccggc aacgatcccg atattttgca aatggcgttt tgttttttct
     7381 tctgtttcga ttcgttcgac ttgataaacg aaattctgct ttgcctgcgc taaggtctgc
     7441 atattgattc ctctcttgtg aaataaatca tgtattaaaa tcattcatta tatgttctaa
     7501 ttttcttacg tgttacgcaa ataaatgaga atcattctca catagaatac tctcggtaat
     7561 tgagaatgtc aattatactt gtgatattat gagcgaatct ttagtattga tagataaaaa
     7621 tgagaaaaaa atatttcttc atagttttcc acaatttttt ctctattaaa tttgctattt
     7681 tcccacacga tacttgtttt cagctctttt ttcaaaaaca caaaacttag tattccacag
     7741 tttccacaac acaatatgta gtgtttgttg tttgactttt ttactaagag ttttatacta
     7801 tctattatag ataataagaa tgtgttagag gaggcactca ccatgaacgt aaaaatcttt
     7861 tctaaaaata attgtatgca atgtaagatg gtcaaacggt ttttaacaga aaatcaaatt
     7921 gcttttgaag aagtcaatat cgatgaacag cctgaagcag tagactggtt gaaagcacaa
     7981 ggattccaaa gtgttcctgt cattacttct gatgcagaaa cagttatcgg attccgtcct
     8041 gatcagttgc gtaaacttgc cagctaacat gactaaaaga atcaaaacgt gatacatgga
     8101 tttgaagggc ttgagacagc accagttttt gtcgcaaact tccaaatcct tttttaaagc
     8161 ttaaaaacaa tgcaatgatt tgcaatcaag catcatcaaa tacgaaatga aggttgggcg
     8221 tacgtatgag tcttaaaaat ttacaagatg tcagttattt caagctgaat aatgaaatca
     8281 atcggccagt caatggtcaa atccctttga ataaggataa agaagcttta gttgcctttt
     8341 ttgaggaaaa tgtcaaacca aatacgatga cttttctatc tattatggaa aaaatcaatt
     8401 acttgatcga tcaagaatat atagaagcag aattcattca attgtattca ccatcattta
     8461 ttgaaaagct atatcaattt ttggacgagc aacatttttc tttcaaatct tttatggctg
     8521 cttacaaatt ttattctcaa tatgcgttga aaaccaacga cggttccgct tatctggaaa
     8581 cttatgaaga tcgagtagcc ttcaatgcgc tttactttgc gaacggggat gaagaattag
     8641 cacttgctct tgcagacgaa atgatccatc aacgatatca gccagctaca ccttctttct
     8701 tgaatgctgg acgtaaacgt cggggcgaat tggtgtcttg tttcttgact caagtcactg
     8761 acgatatgaa ctctatcggg cgctcaatca actctgcttt gcaattgtcc cgtattggtg
     8821 gcggcgtagg gattacttta tcaaatcttc gtgaagccgg cgctcctatc aaaggatatg
     8881 agggtgctgc aagtggtgtc gttccagtca tgaaattatt cgaagatagt ttcagctatt
     8941 ctaatcaatt aggtcagcgt caaggagctg gcgtggttta tttgaacgtt ttccatccag
     9001 acattattgc tttcctttca gcaaaaaaag aaaatgcaga tgaaaagatt cgcgtaaaaa
     9061 ctctttctct tggcgtgatc gtacctgata aattttatga attgacacgt aaaaacgaag
     9121 atatgtattt atttagtcca tatagtgtcg aaaaagaata tggtgtccca ttctcttatg
     9181 taaatattac agctgagtat gatcatctag ttgcaaatcc aaacattcgc aaacaaaaaa
     9241 tcaaagcgcg tgatcttgaa aatgaaatct caaaattaca acaagaatct ggctatcctt
     9301 acgtaatcaa tattgataca gcgaaccgcg agaatccaat cgatggcaag atcatcatga
     9361 gtaatttatg ttcagagatt ttacaagttc aaacgccttc tgtgattaac ggcaaacagg
     9421 aatatgaagt attaggaacg gatatctcat gtaacttagg ctcgacaaat atcgtgaatt
     9481 tgatgcaaag ccctaacttt ggtcaatctg tgcgtacgat gactcgtgca ttgacattta
     9541 ttacagatgc atctgatatc gatgtcgtac caccgatcca aaacggaaat aagctgaatc
     9601 atacgattgg attaggtgcc atgggcttac acacttatct tgcaaaagaa caaatggaat
     9661 atggctcaga ggattcatta gactttacaa atatttactt catgctgttg aattattgga
     9721 cacttgttga aagcaacaat atcgctcgtg aaagaaaaca agttttccat aattttgaaa
     9781 aatcagctta tgctgacggc acttatttcg aaaaatacgt aactggagaa ttccagccaa
     9841 aatcagaaaa agtcaaagca ttgtttgaag gcatttttat cccaactgct gaagattgga
     9901 atgctttgaa acaagcagta atgaaagatg gactgtatca tcaaaatcgc ttggcagtag
     9961 caccaaatgg ctcaatttca tatatcaacg atacgagtgc aagtattcat cctatcactc
    10021 gcatgatcga agaacgccaa gagaagaaaa tcggtaaaat ttattaccca gcgccttatc
    10081 tatccaatga gacgattccg tattacactt ctgcttatga tatggacatg cgtaaagtaa
    10141 tcgatgttta tgcaatggca caacaacacg tcgatcaagg catgagtttg actttattca
    10201 tgcgttctga aatcccagaa ggcatttatg aatggaagac aacaacgaag caaacgacac
    10261 gtgatttgaa tattttaaga cattacgctt tccacaaagg aatcaaatcc atttattacg
    10321 tacgtacctt tactgatgat gcagaagaaa tcggaagcaa tcaatgtgaa agctgcgtga
    10381 tctaatatca tagatagatt cgcttattca tattttaatt aaaggagcat tctcatgtca
    10441 gaaacatatt atgaagcaat aaactggaat gaaattgaag atatcattga taaatccact
    10501 tgggagaagc tgacagagca attttggcta gatacacgta tcccactttc aaatgaccta
    10561 gatgattggc gtacattgtc tcaattggaa aaagatacag taggacatgt atttggcggt
    10621 ttgacactgc tagatactgt ccaatctgaa agtggcatgg atcagttgcg taaagatgtc
    10681 cgcactcccc atgaagaagc cgttttgaac aatattcaat tcatggaatc cgttcatgcc
    10741 aaaagttatt cttctatttt tagtacactg aatacgaaaa aagaaatcga agaaattttt
    10801 gactggacaa atacgaatcc ttatctgcaa aaaaaagctg agcgtatcaa tgaaatctac
    10861 aaacacggta caccgctaga aaagaaaatt gccagcgtct ttttagaaac attcttgttc
    10921 tattcaggtt tttatactcc tctttattat ttaggaaaca acaaattggc taacgtagca
    10981 gagatcatca aactaatcat ccgagatgaa tctgtacatg gaacctacat cggttacaaa
    11041 ttccaattag gattcaacga gttacctgaa gcagaacaag aaacgttaaa agactggatg
    11101 tacaatcttc tttatgagct ttacgaaaac gaagaacgtt atacagaaga attatacgat
    11161 ccaatcggct ggacagaaga agtcaagaca ttcctacgct ataatgccaa caaagcattg
    11221 atgaacctag ggatggatcc actctttcca gatactgcca atgatgtcaa cccaatcgta
    11281 atgaacggga tttcaacagg cacaagcaac catgacttct tctcacaagt cggaaacggc
    11341 tatttgttag gtcatgtaga agcgatgaaa gacgacgatt atttgattgg attggaataa
    11401 ggatttattt taattcaaaa atttttcgaa aaataaaaag agtttagcgt acagatattt
    11461 taaatgtaca ctgaactctt ttttatgctt attcacttgt ttcttccagc acttttggac
    11521 tgtctaactt ttccgaaaca tgttccattc ttctcaataa atagattttc cggatattta
    11581 atattatcgt cttaatcact gcatagacag gtatgcaaat cagcatgcct aatattcctg
    11641 cgacacttcc tgctccaatc aataccaaga tgatcgtcaa aggatggata ttcattgatt
    11701 tcccaaataa caacggcttg atcaaatttc catctaattg ttggatcact aaaatcgaca
    11761 acgccatata caaagcttga aatggagaga taaacaaacc gacaatgatt gctggtaccg
    11821 ctccaataaa agggccgaca tacgggataa tattcgtgat tccgcaaatg accgcaagca
    11881 acaagctata aggctgatcg aatattctca ttgccaaaaa gctcattaca ccaataatca
    11941 aagcatctaa gatcgtactg ctgatatagg cagataatgt ttcgtttaat tcttttaccg
    12001 tttggcgtaa ctctttacga atcgctttcg gaaagaagtg agacaacgca tcaataaatt
    12061 tgtgcccatc cttgaacata aatagcaaaa taaatggaac ggtaaataac aaaataaaaa
    12121 attgcatcat aaatgaaatg atttttgaga cacttgcaga taaactgact aatatgatat
    12181 tgaacaaatt actaagagat aaatttgctg atgctaattg ttcttccaaa ttgaaatttt
    12241 taaactcttc ccgctgcgcc aattcattta accatttgct tgtttcatct gcatacattg
    12301 gcaagctctg ggtcaattga attgtctgtt ccattaactg aggcactaca ttcatgacag
    12361 ttaaaacaac taaaatgatt acgaccaaaa acgtcaataa aaaaccgaac actctcggga
    12421 tccgatgctt ttcgagaaaa acaacgattg gatcgaacat ataatagata aaaccagcaa
    12481 caagcaatgg taaaaaaact gccgaaagca ttttcaatat tggactaaaa atattcggca
    12541 tttgtaaaat aaagtacacg ccgatgatcg tgagtactag ctccactgtc cagaaaaata
    12601 attttgattg tttaaaacgt gattgcatac atctcctcca aggtcctttg atacttctat
    12661 tctatcattt aacaaagagt tacgcgctga gattttcatt tgcttaaaaa aaacaaaaaa
    12721 aataaacata ttttaatgag tagacgagta cttaaacaaa aacacggttt ggagtagtga
    12781 caaaaattat actgcagtcc ctttttccaa ataaacggtg ttcaaaagct tgcatcttcg
    12841 taataagccc agccaaacga aaaatataag gaactatttt tatttggctg ggcttattac
    12901 ttgatgtagg acatctattt tacaacctca gatcatttat tgcgcaatac tgactaaatg
    12961 caactttgtg atatttttgt gtacggacaa ctcctcgatc acatcggcat aagtgagccc
    13021 ttttggaaga tcaatcgtat agatattttt gtacactcgg tagtcatcaa cgaattttac
    13081 gtcaaaattc acatcttcga ttacgatatt tttctcttca aaataatcgg taagaaaatc
    13141 tttggtttct tttctatgga taaattggat ttctaacttt ttagtcgtag gtacatggat
    13201 cacgcgtttc actactgtga gtgcaatagc aatcccgata aaactgatga tcgcaatgga
    13261 atagtaaccc atgccgatgg cgatccctaa tccagcaatt gcccataaag aagcggcagt
    13321 cgtaagtccc gtcaccgatt gtttcgtgac gatgatcgtt cccgcgccta agaaaccgac
    13381 accactcacc acttgagcaa tcagccttgc ttcatctgaa cgaatcgttc cagcaagctg
    13441 tggatactct aaagcatctc ttaatgcacc actagcgatc tcaacttgga tcaatgcaat
    13501 gatacatgct cccatacaaa ctaaaatatg cgtacgcata cctgctggac gatttttata
    13561 ttgtcgttca aaaccgattg ctccgccaaa tagcatagcg atccctaaac gtaaaataat
    13621 ctctggaatt gtcaatacca tatcattcat ctaaacaccc tccagtccaa tatatcatgt
    13681 ctgaaaggag cagacaaaaa aagtgcaatt tttacctctt acgaaaaaag gactgtgaca
    13741 aaatttttgt catagtcctt tttatgaata aacggtgttc aatcagctga ccctcggtat
    13801 tactcaacaa acggcaaaat atggggagct atttttcctt tgtcttctct aatactcagg
    13861 tcaaaacgct gatttcacaa cctctttagc ctactattga aaattacaac ttacttgctg
    13921 cttcttcatc gatgatcaca acaacattgt cgtgattttg caatacactt gctggcaaat
    13981 gattcgttac aggtccttca atcatccctt tgattgcatc agctttgctt tcgccaaatg
    14041 ccatcaagac gatttcttta cttttcataa ttgaaccgat tcccattgaa acagcttttg
    14101 ttggtacatc ttctgcttta tcgaagaatc gtttgtttgc attgattgtt gattctgtta
    14161 agtcaactac tgaagtcgtt ccgtcaaatg aagcaccagg ttcattgaaa ccgatatgtc
    14221 cattttgccc gattcctaag atttggatat cgattggatg tgcatcgatg atttgatcat
    14281 actttttgca tgctgcttcc agatcttctg cttttccatc tggtacaaac gtttctttga
    14341 atggtttttt atcaaataat tggacatcca tgaaatgacg gtagctttgt tcatctgaac
    14401 cgcctagtcc aacgtactca tctaagttta cagaagtcat ttcactgaaa tccaaatcgc
    14461 ttgaagtcat ttctttatat aaagtaactg gtgtgcttcc tgttgccaat cctaatactt
    14521 ttgcaccttt gttcatacct tctttgatga tttcaaatgc tttttttccg ccttcttcag
    14581 cgttttttac acgaatgatt tccatagtga ttgctccttt atattttggt atagcccaat
    14641 tatagcattc tttttttctt tttacaataa ttagtataga ccaaattgca tttttttcat
    14701 tttcttgaca ataaatagga atccgtgtta ggtttcataa gatggttcaa tcaaacaatc
    14761 catgttgcat gaaaaccaag caagtgattc actgtggacg ctttatgtct gcagtttttt
    14821 tatgaaaaag gagttattat gagtatactg acattaaaca acattaccca gcaatttggc
    14881 gacaaaatcc tctatgaagg tgtcgatctg caattaaatg ctggcgaaca tcttgggctg
    14941 attggacaaa acggagccgg taaaagtaca ttgattaaaa tcatcactgg tgagatctta
    15001 ccagatgacg gacagatcca ttggcaaaaa aatattcaca aaggttacct cgatcaatat
    15061 gtggaagtcg atgaaacatt aacaatcgaa gaatttctaa aaactgcgta cgcaaaagaa
    15121 tacgaaaagg aagaaaaaat tgcttttctt taccaaacat atagcgagac cttcgatgat
    15181 tccttgctgg aaaaagctgg gaaactccaa acggaattag accaaggagt gttttatcaa
    15241 atcgacacct tagttgctga gatgagcagc gggttgggga tcgatgtctt aggtttagat
    15301 tcactgttga aaaatctaag tggtggacaa cgttcaaagg tgattttagc aaaactactt
    15361 ttagaaaacc cagatgttct gatcttagat gaaccaacca atcacttaga tgatcagcat
    15421 atccaatggc tgacgcagtt tcttcaggat tttgacggtg cgtatatcgt gatttcccat
    15481 gaccaagaat ttcttgatca gatcaccacc catatcgcag acatcgagtt tggaaagatt
    15541 actaagtata ctggccatct aaaacaagca ttgaaacaaa aggaacaaaa tcgcgaaagc
    15601 tatttgcgcc aatatcatgc tcaacaaaaa caaatcgaaa agaccgaagc ttatattcga
    15661 aaatacaaag ccggttcccg atcaacgatg gcaaaaagcc gacaaaaaca attagataaa
    15721 atcgaacggc tgacgcctcc agcttcagca agtaaagctg tctttgattt tccattctcg
    15781 cctattgtca cgacattagc agtggaaaca actgatttag tcatcggata tgatcgaccg
    15841 ctacttgcac ccatcaattt gaccatccgc tttggtgaaa atattgccat tcgaggcttc
    15901 aacgggattg gaaaatctac tttattaaaa acactgatcg gtgagattga aaaaataagt
    15961 ggcgatttcc acttcccaga taacacaaaa atcaattact tttcacaaga tctgtactgg
    16021 gaaaatccat tagaaacacc actacaatat ttaagcagtc aatttccaaa agcgactatc
    16081 aaagaattac gtcgccaact agctaaagca gggttagtta atcaattagc aagtgaacct
    16141 ctcactacat taagtggtgg ggaacaaact aaggtgaaac ttgcacagtt gacaatgaac
    16201 caaggtaatt tactgattct cgatgaaccg acgaaccata tcgatcaaga aacgaaagaa
    16261 agcttacaag acgggataca gaaatttcca ggaacagtaa tcatcgtctc tcatgaacaa
    16321 gaattttatc aagatcttgt gcaacgagtc attgagattg aaccaaaata aacgtttgat
    16381 tgaatagttg aaaaaaagga gctgtgacaa aagttttgtc acaactcctt cttctgaata
    16441 aatggtggcc aattagctga ccttcggtat tagctcaaaa aacggcaaaa tatgaggagc
    16501 tatttttatt tggctattct tattacttga tgcagaacag cttttcacaa cctcttttcg
    16561 tctatttttt attttcgaga tttctctttc ctttaaaaac aactgtttga aagaagaacg
    16621 cacatccttg aaaaacaagc ccgctaaccg ttacacctaa ccagccatac tgctgccaca
    16681 ttgtcgtccc tagcaatgaa ccagccgcac ctccaataaa gtacataaac ataaatacag
    16741 tattatttcg gttgcttgct gcttcaccta gcccttgtac tcgtgtttga ttggcgactt
    16801 gaccaaattg attcccgacg tctaataaaa taatagctaa aagcaaaatg gccaaatggc
    16861 ttccgccaac caataaagca ataaagctta ttgtttgcat aacaagtccc atatacacga
    16921 tttttctttc agaataagaa tctgccagtc gtcccacgat aggtgcagca actgctccag
    16981 acaaaccaaa aatcgctaat atccctgctt cgaatgttcc ccaattgtag gcgggactgc
    17041 tgatataaaa aatcaacgta gaccaaaaga tcgaaaatgt tccaaacatg aaaaatccac
    17101 tgactgccgc ttctcttaat agacgttgag aagcaactaa atgcggcaaa cttttaagtg
    17161 aatcaaggta tgaaacagct gtactacttt gctggacaga acgcggcatt ttccagtaaa
    17221 gcagaagtgt aagaattccg actgcacata aagcaaataa atagatcatc cgccaagaaa
    17281 aaagactggc aacaatacca gataccgtcc gagaaagcaa aatccccacg agtaaaccgc
    17341 taagcaatgt acccatgacc ttccctcttg cttttggtcc cgctagtacc gcaccgtaag
    17401 gaatgatgat ttgcggcacg atcgaaagta atcctattaa aaaagaagcg catgcaaata
    17461 acgtaaacga aggcgcaaaa aaagcggcca acagagagac ggcagataac gctgccattc
    17521 gaatgatcaa ttttggacga tccaccacgt cccctaaagg aactaaaaac agtaacccta
    17581 acgcatagcc taattgggtc aacatcgtca atatcccgac tgcacttgtg ctaacagata
    17641 aactagctgc tacttttgtt ccaatcggct ggatgtaata catattggca acaacaacgc
    17701 cacaagtgat tgataatagt aaaataagcg aagttgttaa aggttttttt tcattactct
    17761 tcattgattt cccccatttc ctatcttagt ttacaactta tggaaaagat tggaaataga
    17821 aaaatacaac tattcgccac acttattatt tgtgcttttg ttttccttgt tttttcttgc
    17881 gatctttata aatgaagaca actaacccta ctagcacagt aacacttgca accaacgtga
    17941 ctgcagtttt gttatatccg tgaaacagag cttctccacc acacgcatac aacacagacg
    18001 aagggatgac tccaatcacg atcgacaata tgatttgctg tagaggcaat ttcatcgcat
    18061 ctgcagcaaa attgaccata gaagatggaa taaccggtat catatagcct aacattacac
    18121 ctatttttgg atgtttcatt tgtcggattg cttgcaccca atgattcgtt tcggttttct
    18181 tatctaaaaa ttttaagcga tgcataagga aaattgcagt gatgttacca agagcattac
    18241 ccaaaatgtt gatgatcgca cctgtcagtg gtccaaagct caaaccagca atgactccaa
    18301 ctactgaagt aggaatacct ggaacagcac acatcaccgc tgtcaatacg atcaagagta
    18361 tcgatgcaaa agtcccatgc gaacgaatat gtgcgagtag ctctgcctta ctcgctctcg
    18421 gatttaaaaa taattggatg tctgtctgat attctaaata caaccgatag cccactacca
    18481 gtatcagaat gattcccaac gagatcaata tcaatcggat tattttcttt ttcatatttc
    18541 ctcctgaaaa aaatctatat tcattaaata atagtttaac ataaaaacaa aaaagcaccg
    18601 aaatccaaca taggatctca gtgcttttaa atagtaaaaa ttatttaaca gttacagatg
    18661 cgccaacttc ttcaagttta gctttcaatt cttcagcttc ttctttagat acgccttctt
    18721 taactggtgc aggagcgcca tcaactacag ctttagcttc tttcaagcct aaaccagttg
    18781 cttcacgaac tgctttgata actttaactt tttggtctcc agctgcagtt aattctacag
    18841 tgaattctgt ttgttcttca gcagcagcag cgccaccagc agcagctaca gctacaggag
    18901 cagcagcaga tacgccaaat tcttcttcaa tagctttaac taggtcgtta agttctagga
    18961 tagttgcttc ttttaactca gcaacgatgt tttcaatgtt caatgccatt ataatttcct
    19021 ccgtttaatt gttctttttt atttaatgct ttatgcggca attaagctgc atctccatct
    19081 tctttgtctg ccactgcttt gacagcgtaa gccacgttgc ggactggcgc ttgtagtaca
    19141 gatagcaaca tagatagaag tccttcgcga tttggcaatt ttgccaatgc tgtgatttct
    19201 tccaatgaag agactttacc ttcgatgata ccgcctttga tttctaatgc ttttgcttct
    19261 ttagcaaatt cgtccatgat tttcgctggt gcgactacat cttcgttact gaatgcaaca
    19321 gctgtaggtc cagtgaatac ttcgtccatg ccttcaaggc cagctttttt agctgcacgt
    19381 gaaagaatag aatttttgat aactttcatt tctacgttag catcacgaag ttgtttacgt
    19441 aaacgtgtta cttcatcaac tgttaatccg cggtagtcaa cgactactac tgatgcagct
    19501 gctgtaaatt tttcgcttac ttcatcaacg attgctgctt tttttgcgat aattgcttca
    19561 ctcatttttg agtttcacct cctgaatttt gatggaagag ttttttgcac tgaaaaggca
    19621 agtcactttt atagttactt tgctcaacca gtattattca tcacctattc acttcgttca
    19681 tagtgattct actcgctttg ctcgaaagga aactcctttc gctttgctca accagtattg
    19741 ttcatcatct cttcacttcg ttcatagtga ttcacaataa aaaactctat gccaccgcag
    19801 acatagaggg actattgaat atattcaata aacgtcctcg gtaggaaatt aaggcttgat
    19861 gccacctact gtcttcggta caagttaatt attaacctca actagcttag catgctaaac
    19921 tagttgagtc aagaattatt tttcagttgg atcaatcaac attctaatat ttaattagaa
    19981 agaagcttgg tcaacatgga taccaggtcc aaatgttgtt gtgatcgtta catttttcat
    20041 gtattgacct ttagctgctg atggtttagc ttttagcaat acatcatgga ttgctgcaaa
    20101 gttttcaact aatttttcat cgtcaaatga aacttttccg attggtacat ggatatttcc
    20161 agctttgtcc acacggtaag ttactttacc agctttcact tcgtttactg cttttgttac
    20221 gtccattgta actgtaccag ttttagggtt tggcattaag ccttttggtc ctaatactcg
    20281 tcctagacga cctacagtag ccatcatatc aggtgttgca acaactacat caaagtcaaa
    20341 ccatccgttt tggatttttt gaaccatgtc gtcgtcacct acgaaatctg caccagctgc
    20401 ttcagcttct tttgctttgt ctcctttagc aaaaactaaa acagtttgtg ttttaccagt
    20461 tccgtttggt aatacaacag caccacggat ttgttggtct gcttttttag ggtctacgtt
    20521 caaacggtat gcaacttcaa ctgttgcatc aaattttgca atgtttgttt cttttgctaa
    20581 agctactgct tcggcaacag gataaacttt acttgaatca acttttttta atgcatcttg
    20641 catttttttg ctctttttag ccattttggt tcctccttga ttgtggttat aacggttgta
    20701 cctcccacgt gttctatatc tttcaatata gagccaactg agaagctgct gtcttagggt
    20761 gtatagatta ttctacagtg atccccatgc ttcgtgcagt tccttcaacc atgcgcattg
    20821 ctgcttcaac gttagctgcg tttaggtctt ccatttttaa ttcagcgatt tctcttactt
    20881 gatcgctaga aactttcgca actttatttt tgtttggttc gcctgaacct ttttcgattt
    20941 tagctgcttt tttcaataga acagcagctg gcggtgtttt tgtaacgaat gtgaatgaac
    21001 ggtcttcgta tacagaaata acaaccggaa tgatcaaacc tgcttgatca gctgtacgag
    21061 cgttgaattc ttttgtgaat cccatgatat tgatacccgc ttgacctagt gcaggaccta
    21121 ctggtggagc tggagttgct ttacctgcag gaatttgcaa tttaacgatt ttttctactt
    21181 tctttgccac gagacatacc tccttaagtc cgtgatgtgg ttaattggag atgcatattt
    21241 ctcctcccac gttttatctg atctgaaaaa atccagacaa ataatgtgca aaacaatgca
    21301 cactggtaca gtatagcaaa tttatttgtt ttttcaagat ttttttctgc tatttttaca
    21361 ttttgcttga ataaacgcta aatttattct aaaaaaacaa caatttttcg ttctcatttc
    21421 ttcttttatg ttacacttgc gctagcatgg ttcaacagat gaaagcaaac aataacacgg
    21481 ttttttagac ccttgtacaa agattcctac ataacaaaat gaggtggaga aaatgaaaga
    21541 tttaaggtat agaagtgtct ttgatattat tggacctgtg atgattggtc caagtagctc
    21601 tcacactgcc ggagctgcta gaatcgggaa aatcatacgt agtatatttg gcgaacagcc
    21661 tgattctgta gatatttatc tatatgaatc atttgcaaaa acctatcggg ggcacggaac
    21721 agatattgca ttagttggcg gcctgttagg gatggaaccc gatgatgaac gtttagctga
    21781 ttctttgaaa ttggcatatg aagccggtat ggaagtcttg tttgttccta agagtgaaaa
    21841 agccgaacat ccaaactctg taaagatgct tctaaaaaaa ggaacacgaa agctttcagt
    21901 cactggcatt tcgatcggcg gtggcaatat ccagatttct gaattgaatg gattcaaaat
    21961 ttctttaagc atgggtactc ctaccttgat cgttgtccac caagatgttc caggtatgat
    22021 tgctaaagtg actagtatac tttctgagac caatacgaat atcggcacta tgacagtgac
    22081 acgggaatca aaaggtgaaa atgccattat gataattgaa gtagatgatc ctcaagtaga
    22141 agaaacggta aaaagactca aacagctgcc aaatatcgac agcgtaaatt atttcgatta
    22201 aggagctaaa aaaatgtttt tatcgattga agaattagta gatcaagcaa aagcttatcc
    22261 aagtgtagca gaattgatga ttgcagttga aatggaaatg agtggccgtt cccgtagtca
    22321 catcatcgag atcatggaaa aaaacctctc agtaatggaa caatcaatca acgagggaaa
    22381 agaaggtgtt acctccgtca caggaattac cggtggtgat gcaaaaaaat tagatgatta
    22441 tttaaatgag gcagactttt taagtggaga aacaatctta aaagctgtac gcaatgctgt
    22501 agctgttaat gaagtcaatg caaaaatggg gttgatttgt gctacaccga ctgccggaag
    22561 cgcgggtgtt gttcctggtg tgctgatggc tgtccgagat cggcttgatt tgacacatga
    22621 acagcaattg gaattcttat ttaccgctgg cgctttcggg ttagtcatcg caaacaacgc
    22681 ttctatcagt ggtgcagaag ggggatgtca agcagaagtt ggttctgcaa gtgcaatggc
    22741 agcagcagct ttggtttgtg caaaaaatgg gactcctcat caagcagcac aagcagtagc
    22801 cattacattg aaaaatatga tgggactgat ttgcgatccc gttgcaggct tagttgaagt
    22861 cccgtgtgtc aaacgtaatg ctttaggctc ttctcaagct tttatctctg ctgatatggc
    22921 attagcgggt atcgaaagtg tcattccgcc ggatgaagtc gttgctgcaa tgtaccaagt
    22981 cggacgtcaa atgccgtcga ttttcaaaga aacagcagaa ggtggcttag ctgttacgcc
    23041 aactgctaaa cgacttgcgg aagagatttt aaataagagg gcgtgataaa gctccttaat
    23101 ttcgagtatt aagagattag ttatcttcat aacaaaaaac gaagagagga ctaattgccc
    23161 ttctcttcgt tttttattaa tccatcaatt tcgtattttt tacttcatcg atcgtttttg
    23221 ttcctgcaag ttgcatcgtg atcgacaatt ctttgttcag gtgttcaaaa acagatgtta
    23281 ctccttctgc cccacctaga ttcaacccat aaatcactgg tcggccaatc gcaactaagt
    23341 cagcaccact tgccagagct ttgaagacat gctctcctcg acggacacca gagtcgaaaa
    23401 tcactggtac acgtttgttg actacttcgg caatttttgg caatacttca aaggacgcag
    23461 gacctccatc tagttgacgt ccaccatgat tggataccca gataccatct gcccctgcag
    23521 agatagcgat tactgcatct tctggcgatt gtattccttt tacgataact ggaagatggg
    23581 tgatttcttt gattgttttg atgtcatctg gtgtcaagcc ttgttttgca gctgcataaa
    23641 tttctgcgat tccttttcct tcgccattac cacttgcact ttgttcgcta tacgcagcta
    23701 agttaggcat tggtaatgga aattggaatt gattgatgac gtcttcttca cggtaaccac
    23761 cgagcgttga atctgccgtt aaaatgatag ctttagcccc tgcttctact gctttgttca
    23821 aaatgaactc attgaatctg tcgtctttac tcatatataa ttggaaaaac tgcggtgcgc
    23881 ctggcgctgc atcggctgcg tctttgattg tcgtatttgc ataagtgctg atggaaaaga
    23941 ttgatccagc agcagctact cctttagctg tatcagcttc ccctttttca tgtgccaatc
    24001 cttgggccgc tgatggcgct tggataatcg gtgtatctaa ttcaattccg aaaacagatg
    24061 tatgcaaatc ggctgagtcg attccgcgta atatccgtgg catgattttc tttgtattaa
    24121 aagaagtcgt attttctttc atcgtccact catcttcaga accaccgcga atatagccaa
    24181 aagctcctgc ttccatgcgc tcttttacac gaccttctag ttaggcaatg tttacaatat
    24241 cgattggatg ttcttcatta cttgtatgat aagtcatagt agatccttct ttcatcttct
    24301 tgattacata taaatcatac taaacttaca aaaagtaagc aaacatttac tctcagatac
    24361 ttttatcgct attctttcca aataaatcga cttacaagct gatctacttc ttggttttca
    24421 tgaagcatac tatgagaggc attttcacct gtcactactt tttcctctat ttcatttccg
    24481 cgttgcttca acaacgcata gaccgctaat gcactgttta acggtactgt tccatcactc
    24541 aagtctgttt catctagttg accagcaatc aagaaaaatc gagtggttat aggaatatta
    24601 ccgatcattt gctggtaatc ttgatagcgg ttagaaacga cagcaggccc ctttgctaac
    24661 tcatctgtca gagactgcgc actatcatcg acaaaatcat taaaaggcgc gccaattgct
    24721 gttaatgtct gaacttgtgg caatgacgta tcctgtccat acgttcctaa atagcgtagt
    24781 gcactaactc ctcccatgga atgccccact atgttgactt tatctatgcc gtagttttct
    24841 ttcagataca aaagacaagt tttgatccat tctgcttgat tccactcgtt atttttatta
    24901 tcttcaaaca atacttggac catcggattg tctgcttttc cagacaaatc tcctgttgct
    24961 tgtatttgtc catctgttcc tactgttaaa accaattctt ttactgcttg ctgatttttt
    25021 tccaaacgcc gcaacatccc tttaaaggaa ttttctgtcc cgctgtagcc gtggaaaaaa
    25081 agtgttggaa tagtggtttt ttctatcact ttactggatt ctgccgtttt ttctttatca
    25141 gaaacttcag tagtatttat cttcgtcgtc tggcaaccac ttaacgcaaa caaactgata
    25201 aggactaaag ctgctgcagt tgcacgtttg cataaatttt tgattggttc tgtcatacac
    25261 ctcttccttt ccatatagaa aaaagaaaga cttgaaaggc caatcatatg atcgacattt
    25321 caagtctctt tattaatcga ttttatctac ttgatcgaaa tcaagttctg tactggtttc
    25381 acgtccaaac atatcgatgt tgactttcaa tttttctttt tctccgtcaa cttctgttac
    25441 ttgtccttct aatccagcaa atgcgccttc gatgattttc acgacttcgc caacttctac
    25501 ttctagatca ttttgtcgca ggctcatacc gatagaacgc aagatatgat tgacttcttc
    25561 tggaagtaat ggtgctggtt tgcttccggc tccgtgtgaa ccaacgaatc ctgtcacacc
    25621 aggtgtgttg cggacaacat accatgaagc gtctgtcatg atcatttcaa caagtacgta
    25681 tcctgggaat gttttatgaa cgatttcttt ttctttccca tttttgactt cagtttcttt
    25741 ttcttcagga acaacgacac ggaaaataaa gtcggccatt ttcatacttt gcgcacgaga
    25801 ttcgatattt gcttttactt tgttttcata tcctgaatat gtatgcaaga cataccagtt
    25861 tttttcaaaa gattccattg aaggctcctc atttcattgt aaaataaaaa aacctcagta
    25921 cacccgaagt ttttctttcg cttcattata gcactaaaag tacgttgtgg ctagcgagtt
    25981 attttaaaat ccagccaaat aagctttgga tggctgtatc cattacgtaa aacatcaatc
    26041 cgaaaataat agaagtttcg atgactacta acgtatcttt acggagttgt tttctgcttg
    26101 gccaagttac ttgtttgatt tcatctttta cgctttttag aaatttcatg agacaacctc
    26161 ccttgctact gtttatttcg tttctttatg taatgtatat tgattacaat atttacaaaa
    26221 cttattgatt tccaaacgtt cgccgctttt tccttcactg actgattttg tgtagttgcg
    26281 agacccacaa acggaacaag cgagtgctgc ttttttcttt gccatttact tctgccccct
    26341 tgctgagaat cagacttata cttcataaca gtatcacgta actttttact tgtcaatttg
    26401 tgaaataaac tatctattgt tttggataaa attatgatat gattaatttg aaattaagct
    26461 aaaatgagac aattggaggt atagcgatgc tattaaaaac acttgtatat aagaaaaaag
    26521 atctgacaac cgttacagag actagtacat tagaagaagc attaaaaatt ttagaaactt
    26581 ccggataccg ttgtgtgccg atcttagatt cttcaggtaa tattttccgt ggaaacattt
    26641 acaaaatgca tatctatcgt cataaagcaa atggcggtga tatgacgctt cctgtcactc
    26701 atcttttgaa aaacgctaca aagttcattt acctggatac ttctttcttc aaagtattct
    26761 ttacgatcaa agagcttcct tatattgctg tccttgacag tgaaaatcat ttttatggta
    26821 ttttgacaca tagtacgttg cttaatatgc tctctcaatc ttggagcgta gaacaaggaa
    26881 gctatgtact gacaattgct tccacaggaa agcaaggaga tttggctgct attgcaaaaa
    26941 tcatttcaaa acacagcagc attgccagct gtattactct tgatatcgaa aaagacgaat
    27001 acattcgacg tacattgatc acgctaccgg ctaacactga agcgaaagta tgtgaaacga
    27061 tcgtcaacca cttagagaaa aagaattata aagtggtaga aattgaaaat ttgcaaaacc
    27121 aagaagcata atcaaaaagc gaaaaaagaa aacagctaat ctgctttctt ttttcgcttt
    27181 ttataattag acacgctttt caaaatcaag cgcgcgatct aaataataca tcaatggtgc
    27241 tgaaacagcc atgacgataa actcacttaa tgcaagcgtg ccataggttg tccaaaatgc
    27301 ttggccacct gttggaacta acatataagc aatcagtccc atgctgatgg taaagaagat
    27361 cgtgttcaat actaaacgca tctttacatt tttcactttg ttttgcaata aagctgtcag
    27421 gcctaaagaa agcaatgttt ggccgccacc aaaaaccaca tccatgatac caaaaccaaa
    27481 gaaggcatta tagacgatga ccccgcctaa aactccccaa agcaatttac ggttaaagac
    27541 aactaaatga ttcaaacttt ctgaaatacg aaactggatc ggccctgagg atacaggcgc
    27601 aactaaaata gttaacgcta gataaagagc catgatgatg ccatttacaa cgattacgga
    27661 taaccgttgt ctgcttttcg ttttttgcat tttctctcct cttttctcct agttttttta
    27721 acgcgggatg gttgatgaac cgcggttatg tgaaacaaac aataattata acagactttt
    27781 catttatttc aatccgtctt atctgctttt aaaaaagcaa aagcttaaac aatgtaaacg
    27841 tttgctaaaa atcgtgggat atggtataaa gtaagtgtaa ggggtcataa gaattcccag
    27901 taaagcagac gaagtaggat ctgcaagaac agaattgttg tgaaacttaa ataaataaaa
    27961 aaggatgtgg gaatttgagc aacctcttac tagagaacgg tctagcaagc agctgaagat
    28021 ctaagtggac atgccgaacc ttggaacttc tccttacgta agtaatggat catgcgcttg
    28081 ctaggctttt ttgtgttttt cggtcttcgg acctttttca ttttcctaga cttctgctat
    28141 aataaccaag aattaatttt gtaacagatt tttttgaaaa aacaagtttc tttcattcaa
    28201 aaaatagaaa aagcttgcta attattggtt aaccagcaaa caggagtatt aaaatatctt
    28261 gttttattga atagcaggag gaaatcatga aattccatta tcatatattg atcaatccat
    28321 cggcaggcag cggaaatggc tataaagtag cagaaagaat tttaccggta ttaaaaaata
    28381 agcatattga ctatacactt cattatagtg aatacaaagg acacgatgct gagattgctg
    28441 aaactttggc aaaagaaaca ttgattcctt ggatagaaga agaacagaaa acaatcgata
    28501 ctttccccct tttgatcatc gtaggaggtg acggcacctt acatcaggta cttgatactt
    28561 tctatcagat ggaagtagaa ttcccagttg cttatattcc agcaggttct ggagatgatt
    28621 ttgctcgagg ggcaggcctt ccaaaaaatc ccaaaaaagg acttcagctg attttagcag
    28681 ctcaatcacc agaaaaagtc cacataatgg cttatgaaga aaaaataagt gaaaaaaaag
    28741 gaatagctgt taataatttc ggtataggat tagatgcagc tatcgtccat gcgacaaatc
    28801 attcttccac aaaaaagcga ttgaacaaat acaacttagg atcattttcg tatttattct
    28861 ctattctccg tgctttattc actcaaaaag gatttccaat tctggtagat gcaggaggaa
    28921 aacaattaag tttttcaaac gcatttttat gtacagcaac gaagcatcct ttttttggcg
    28981 gaggaatcgc gattgttcca actgctgatg cgtctaaacc agtcattgat tttgtcgtgg
    29041 tcgaacgaat caatttcttt aaaatcttgt ggctcattct tttacttata cagaaaaaac
    29101 aattgaaatc gaaatatttc catcactaca caacaaacag attacgtatc gtttcaacaa
    29161 caccgcaaca tggtcaagaa gatggagaag aaatggaaaa acgacctttt gatatcacaa
    29221 taaccacaac taaacagttg ttttggtgtt aataaaagag gttttgtcag aagctacatc
    29281 tggaacaccg tttattcgga aaaaaggact gtgacacgac ttttgtcaca gtcctttttt
    29341 tactatgcag ttaattcttt gcccaattgg gttgccatcg atagtttctt tctttaccca
    29401 aaataaattg gatcattgaa aacaccgcag caaaagcaac taagacacaa ccattgaacc
    29461 aaaaagattc tgcaaatgtt tgattgtaac tgagtgtttc actgatcaag ttattatttt
    29521 gaacaaacca atatgttggt gtgaatgccg ctattttgct aaccatttct ggcaagtatt
    29581 ctgctggtac aaacacccca ccgataaagc aactgcctgt gataaaaata tctttgatcc
    29641 caccgctggt ttctgagttt tttaccaatg aagtgataca agccgaaaag ctaatgatcg
    29701 gcaataaaaa caagaaagta tttaaaatat gataccatac cgttatatcc cacgaggtgt
    29761 ggatcactaa cagaaccatt agaagtaaga agccactgaa caacagcagc gagtaactaa
    29821 tcaaactaaa ggttactttg cgattcaagt ttcgtttggt aatgggcgca ctctggtttc
    29881 gtttactgat ctctttgcga ttaaatgcta gattgatgac gctgaaaccg ctaaaaatgg
    29941 atagaaacaa cccataagac aagaaattaa aggttgtgcc cgtcaacagc tttttcatcc
    30001 gctgactata tcccgcactt agctcaactt ctccagtaac cgagagtgcc atttgtgtct
    30061 gttccatagc tgcatcgatt gttttatctt cttcttgaaa cagaatcagt gtttgcaaaa
    30121 aggtgttcac ttgctgtgta acaactgttt tagaaaaggc atctgggcga ctctgtatct
    30181 tgatcgtcgg catctttcct acttcgactt gttcggtaaa gtctgcaggc agatgtaaga
    30241 taatgtttac acgattaaaa taaagagcat catcgatttc tttttgtgag gttccatcca
    30301 atgtaacgac tgtttccttg tcattcaaat atttacgaag gccatcgact acaggcgact
    30361 cttcttcact aatcaaggcg attttcgcat cttgcagaga ggtattttca acatcaatat
    30421 tttgcgcaaa aaagaatgtg gcaacaacag tgataattac acctatcagt aaattcgatt
    30481 tatttgcttt aagcaatgtc caaatcgttt tataaagcgt cataacgcac ccctctttcc
    30541 atccaagcat ttcctaacgc aaaaaggaaa ataaatccga tcaaccatag aagattggta
    30601 taaaaactag ccaaatcagt gtagaaataa agtttaggcg gattttataa tgaacttagc
    30661 catttaaaat aaaaaaaacg ccacgtgaat tccatgttat attgaagtta ccacaactct
    30721 caatagacaa ggatgaattc acatgacgca aacccatgat aacacagttt cacgcaaggg
    30781 aaaacatctt tcctactcag aacgttgcca aatcgctatc ctgaaaaagg aaggctattc
    30841 caaccgtcag gttgccaaag tgctgggacg tgttccgcaa accataaaca atgagattga
    30901 tcgcggaacc atcacccagt taaaacgcca aacgcaaaat ggaaaagtgt atgacaccta
    30961 ctattctgtt tatgacgctg atgcaggaca ggccttctat gaaaagcaac gactgaactg
    31021 tggcagacgc cccaaatggg cgaacacaaa tgccttcatt gattgggcag atgacaaaat
    31081 gttaaatgaa aaatggtcgc cggacgtcgt ggtcggattt gccagaaaac acgagctgtt
    31141 cgattgtgcc atcattcctg gcacaaccgc tctttatcaa tggattgacc gcggaatcat
    31201 gcgaacaaag aacatggact tgcttgaaaa actgtcccgt aaaatcaaag cggtccgccc
    31261 aggacaccgt cccaacaagc gtgttctcgg gacgtcaatt gaagaacgcc ctaaagaagt
    31321 tgaaacacgg gaaacgttcg gtcattggga aatcgacacg gtagtgggaa acaagaccaa
    31381 gtcggatgcc gtgctgctga cgctggctga acggcagaca cgctttgaag tcatcctaaa
    31441 gttgaacggg aaggacaccg aatcggtgga ccaagcggtt caacagctaa gagaacgggc
    31501 tggcgctaac ttcgatcaat tatttaagac cgttacatcc gacaatggct cggagttttc
    31561 tgggctacac gatgctctgc aagatgtagc tgacgtctat ttcagccacc cctatgcttc
    31621 ttgggagcgt gggacgagcg aaaaccagca caaattaatt cgccgtttcg tccccaaggg
    31681 aagcccgatt agtgaggtga gcgagaacca gattttgcgg atccagcgtt ggatgaacga
    31741 ttacccgcgg aaaattttgg gttatgcgac accacatgat acattcgtcc aggcattcaa
    31801 gcaagaacgg ttggtggctt gacccattat tccttgataa cgatcctcac tcgggacatg
    31861 gcttcatgtc tggaaagctg gcctggggcc agaatcgtct gaagactatc atacctcagt
    31921 tcattatcaa ggcctcactg atttagaata acgaggcagt tacggtatca aacaaatgag
    31981 ttacctgt
//
LOCUS       DARBEC010000025        28779 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.025, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000025 DARBEC010000000
VERSION     DARBEC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.025"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(160..1467)
                     /locus_tag="SCL80_001085"
     CDS             complement(160..1467)
                     /locus_tag="SCL80_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEG1106969.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            complement(1470..2051)
                     /locus_tag="SCL80_001086"
     CDS             complement(1470..2051)
                     /locus_tag="SCL80_001086"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEG1106970.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     regulatory      complement(2167..2264)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            complement(2623..3915)
                     /locus_tag="SCL80_001087"
     CDS             complement(2623..3915)
                     /locus_tag="SCL80_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1106971.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     gene            complement(3912..4535)
                     /locus_tag="SCL80_001088"
     CDS             complement(3912..4535)
                     /locus_tag="SCL80_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106972.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQPDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            complement(4538..5374)
                     /locus_tag="SCL80_001089"
     CDS             complement(4538..5374)
                     /locus_tag="SCL80_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1106973.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            complement(5391..6278)
                     /locus_tag="SCL80_001090"
     CDS             complement(5391..6278)
                     /locus_tag="SCL80_001090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1106974.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            complement(6286..7521)
                     /locus_tag="SCL80_001091"
                     /pseudo
     CDS             complement(6286..7521)
                     /locus_tag="SCL80_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(8204..8887)
                     /locus_tag="SCL80_001092"
     CDS             complement(8204..8887)
                     /locus_tag="SCL80_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEG1106975.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            complement(8905..9522)
                     /locus_tag="SCL80_001093"
     CDS             complement(8905..9522)
                     /locus_tag="SCL80_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1106976.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            complement(9532..10356)
                     /locus_tag="SCL80_001094"
     CDS             complement(9532..10356)
                     /locus_tag="SCL80_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEG1106977.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            complement(10349..10909)
                     /locus_tag="SCL80_001095"
     CDS             complement(10349..10909)
                     /locus_tag="SCL80_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106978.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            11083..12039
                     /locus_tag="SCL80_001096"
     CDS             11083..12039
                     /locus_tag="SCL80_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1106979.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            complement(12344..13300)
                     /locus_tag="SCL80_001097"
     CDS             complement(12344..13300)
                     /locus_tag="SCL80_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1106980.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            13404..14006
                     /locus_tag="SCL80_001098"
     CDS             13404..14006
                     /locus_tag="SCL80_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1106981.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(14094..15593)
                     /locus_tag="SCL80_001099"
     CDS             complement(14094..15593)
                     /locus_tag="SCL80_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1106982.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(15609..16133)
                     /locus_tag="SCL80_001100"
     CDS             complement(15609..16133)
                     /locus_tag="SCL80_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1106983.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            16431..17180
                     /locus_tag="SCL80_001101"
     CDS             16431..17180
                     /locus_tag="SCL80_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1106984.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            17171..17956
                     /locus_tag="SCL80_001102"
     CDS             17171..17956
                     /locus_tag="SCL80_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1106985.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            17925..18641
                     /locus_tag="SCL80_001103"
     CDS             17925..18641
                     /locus_tag="SCL80_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1106986.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(18743..19777)
                     /locus_tag="SCL80_001104"
     CDS             complement(18743..19777)
                     /locus_tag="SCL80_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1106987.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(19781..20839)
                     /locus_tag="SCL80_001105"
     CDS             complement(19781..20839)
                     /locus_tag="SCL80_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1106988.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(21058..22206)
                     /gene="nagA"
                     /locus_tag="SCL80_001106"
     CDS             complement(21058..22206)
                     /gene="nagA"
                     /locus_tag="SCL80_001106"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1106989.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(22282..23118)
                     /locus_tag="SCL80_001107"
     CDS             complement(22282..23118)
                     /locus_tag="SCL80_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1106990.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(23302..24519)
                     /locus_tag="SCL80_001108"
     CDS             complement(23302..24519)
                     /locus_tag="SCL80_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1106991.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(24668..24847)
                     /locus_tag="SCL80_001109"
     CDS             complement(24668..24847)
                     /locus_tag="SCL80_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106992.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            24902..24985
                     /locus_tag="SCL80_001110"
     tRNA            24902..24985
                     /locus_tag="SCL80_001110"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:24936..24938,aa:Leu,seq:gag)
     gene            complement(25225..26595)
                     /locus_tag="SCL80_001111"
     CDS             complement(25225..26595)
                     /locus_tag="SCL80_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106993.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(27309..27599)
                     /locus_tag="SCL80_001112"
     CDS             complement(27309..27599)
                     /locus_tag="SCL80_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106994.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(27807..28499)
                     /locus_tag="SCL80_001113"
     CDS             complement(27807..28499)
                     /locus_tag="SCL80_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1106995.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         8703 a         5984 c         4838 g         9254 t
ORIGIN      
        1 aaaagttagg tcacattcct ttttatggat aaacggtgtt caatcagctg accctcggta
       61 ttagctcaaa caaacggcaa aatacgagga actatttttc cgtttgtttc tctaacaatc
      121 aggtcaaaac gctgatttca caacctttct tttgctctat tacttcttga aggcttcttc
      181 caatatttct tcatcttttt tcaatccatt aaagatcaaa ttcaatatga tagccgtaat
      241 gctactcatt acgatgccat ttccagtgaa catttgcaat gtttgcggca tttgactgaa
      301 taatgtcggc gtaacattga aacccagccc aaatccaaca gaaacagcaa caatcaatag
      361 atttttatca ttgctgaaat caacttccaa cagcatacgg attccttgga ctgccaccat
      421 cccgaacatg actaacattc ctccaccaag taccggctca gggatgattt gtgctaacgc
      481 accgatttta gggaataatc ctaaaacaat caagaagaat gcagagaaat agatgggttt
      541 tcttgttttg attcccgaaa gttgaaccaa tccgacattt tgagaaaagc cagtataagg
      601 gaatgtattg aaaataccgc ctaagataac ggcaagtcct tctgcacgat atcccttttt
      661 caattcttct tctccaacat ttttcccagt aatgtcacca agtgcaaaat atacacctgt
      721 tgattctacc atgctgacca tggaaatgat gatcattagt aaaatcgacc atacgtcgaa
      781 agttggttta ccaaaataaa agggttgtgg aacatggaac aacggcgcat ctccaatcgc
      841 gctcagatca atcattccca tgaaagcagc taagatgctt cctccgacta aaccaatcaa
      901 taccgcgatt gaacgaatga acccttttgc cagcatttgc acgcctacaa ttaggagaat
      961 cgtcacaaat gcaagtagca gatttgtttt gtcaccaaaa tcagctgctg ttgcattacc
     1021 tccacccatt ttctcgattg ctacaggaat caaagtcaaa ccaataacgg tgataaccgt
     1081 tcctgttact ataggaggga atagtttttt aattttagaa aagacaccag aaactaacac
     1141 aacaaagatc ccagaagcga taatagatcc gtagatagct cctactcctt cattggttcc
     1201 gatcaagatc agtggagcta ctgcttggat cgcacatcct aaaacgacag gcaagccaat
     1261 tccgaaaaat cggttaactg tcaattgcaa taatgtcgct aatccacaca taaagatatc
     1321 aattgaaatc aaataagtca tctgttcttg attaaagcct agtcctgtac cgatcaaaag
     1381 aggcaccgct actgctcctg catacatcgc tagcagatgc tgcagcccta aaaccgcagc
     1441 ttttccattt tgtgtttctt tttccacgat tatgcgtcct cctcaagaaa ctcaacttgt
     1501 ccattcgtca aggaagcaat acgcgcaaga gaaacgactc tcaatcccat ttcttcaagt
     1561 aactgacgtc catcttggaa agacttttcg atgacgatcc cgattccttc tacttgcgct
     1621 ccagcttgct ggcataattc gatcaatcct tttgccgctt gtccgtttgc caagaaatcg
     1681 tcgataatca atactttgtc ttctgaagat aaaaatttac gagaaataga aatcgtactt
     1741 gtcacttgtt tcgtaaaaga ataaacagaa gctgtcaata attcttcatc catcgtcaaa
     1801 ctttttgctt ttctagcgaa gatcattgga acatctaatt ctcttgcagc aaataaagct
     1861 ggtgcgatcc cagaagcttc aatggtcaca actttcgtga ttccttgttc tttaaatact
     1921 tctgcaaacc gttttcccat ttgttccatc aatacagggt ctacttggtg agtgacgaag
     1981 ctgtcgactt ttaatacgcc ctcgcctaat atacgtccgt ctttttgaat acgttcaact
     2041 aattctttca ctttatttgc tcctttatca tttattaata ccgaaataac cgcttttgtg
     2101 caaaaaccta ctatgtttct gttaaagaaa aacaccctcc actttatcat aagaaaagaa
     2161 gggtgaaata gccaatcttt tacttatagt ccagcattta cggtactggc tagaaactgt
     2221 catccatatt atgacgatat ataagtaggt atgtaaaact aataatagtg taaattcatt
     2281 ttcttgcaac tgttttgcac gtgaaggcag tttgccatat ttgaataaaa aatgttgttt
     2341 caaatattga aaacagtcta acactacttt ctgaaactga caacgtggtt tacttatttt
     2401 tttgtaaaat ctaatgattt ttaatgcatt attccttttt gttcgggttt aaatagttat
     2461 ttttattttc tcctatctaa aaaagcagcc cacataaaaa gaaggcatga caaaaagcat
     2521 gtcatagccc ctttttccga ataaatagtg ttcaaaagac agcctatcgg caataaggca
     2581 agattttata aactacggga agtaatttcc gtaaatctat tcttattctt ttaagctgac
     2641 aagctcgtca atcactactg cttttcctga agcaatagaa tgatttgctg cgattcctgt
     2701 caaaatcgac aaaataccat ctacgtgagt tgctgcccga ttccacggat cttcttcttt
     2761 ttcttcacca aatagatcat ctaataaacg cggatctcct ccgccatgtc cgcccgcttc
     2821 ttgttcgacg attacctcat atggctcacc gaacatcgga tagacatgga tggactgaag
     2881 aacggctgct ccttcatcct ttttatctcc acctgcattg atgtaagact tttcgatcac
     2941 tttcatttcg atccgtcctt tgctgccatt gaaagaaaca ttgaatcctt cccacggcat
     3001 atacgcgttt agcgaatacg tcaaaatcgc accagtttca tatttgaccg ttacgcctag
     3061 tgtatcttca atagagatat catcggaaaa tacactgcgg tcacgaatat atccactgtc
     3121 tttttctgct tctaaataca tttctcgcaa ttcctcattg gctgacaggt ctatagcaaa
     3181 tggatcagct ttagctagtt cgcttccata gcatcgactg taaaatattt ctactccgcg
     3241 tttctccgca ttatgcatcc cataaaagtt taatccaccg aatgcaaata ccttttgggg
     3301 acggctgttc aaccaaaaat tcactagatc aaaatgatgg gtagatttat gaacaagaag
     3361 cccgccgcta ttgtgcttat ttcgatgcca tcttctgaaa taatctgccc cgtgttgcgt
     3421 atttaacagc cattcgaagt ggatagaaaa cacatcacca atcacatcac tttctatcag
     3481 ttcctttatc ttcgtgtgat acggtgcata acgataatta aaagtgactc gcaggcttct
     3541 tcccgtctgt ttttggaccg ttattatttc ttgagctttt tcctcatcga tagtcagagg
     3601 tttttcgcaa ataacatcat atcctttttt cattgcacga atgatataat catgatgcgt
     3661 tcgatctact gacgtaataa tgacttcagc cggccgtacg tcatccagta gttgctcaaa
     3721 ttgtgatggc atacatgtct ttactttttt ctgtccatac ttgttcacaa gtatccgatt
     3781 tgcataatcc atccttgttt gagatacatc acaaaaaccg actagttcac aagtttgatg
     3841 atacgtagag gcaattgcct cataaaaaaa acgcgctctc ccaccaagtc cgacttgtac
     3901 atattttttc ttcatttaaa gtcattctcc ttttctttta agcaaaatac tgtgacaaat
     3961 ggcagccctg gatacagtaa tgcagtgagt aaaaaatcag gctgtataca ccagaacagt
     4021 ccatacaaaa ggcataaata ccacagaaac cccttgacgt tttttgacaa cagccagata
     4081 ttccttttga tcaaataaac aagactatct gctttatctt ctttgcagat atcaattagc
     4141 gtactaaaaa aactatggga tacaaaaagc aacagacaca atagaaaagg aatcagatac
     4201 caagctccaa tctttcccac taataaatac caatcaactg caaaaataat gacaaacagc
     4261 tgaaacaacg taatgatgaa acacatccgt ttgattttcc ctttcctcca acataccttt
     4321 ttttctgcca agtaatggac agatagataa taagaaggat acatcatcaa aaaataaatc
     4381 agatagacaa cacgagcaga cagcactaat cctaaagtga acaaacatcc tagaactaaa
     4441 gcgaaaagag cattactgat aagactggtt aaaaaaatcc gacagacata gtagttgagc
     4501 ttgtaaagaa acatctcttc tattggcttt ttcatattca gcccttcaga ccgcttgtac
     4561 tgatcccctg gacaagatat ttattgaaga ataagaaaat cagaaaaacc ggtagcagcg
     4621 acaacgtaga catcgcaaac atactcgcgt aagtcgtact agatccagcg tctgcaaata
     4681 atttgatggc aatcgatacc gtgtaagatc tcggcttgtt taaataaatc aatggtccca
     4741 tgaagtcgtc ccatttccaa taaaactgga tgatgatcgt cgtgatgatc gaaggtttca
     4801 gcaaaggaaa aataattttg gtaaagacac tatacttgct gcatccgtca ataatcgctg
     4861 attcatctaa ctctttgggg atgctgacca tgaattgcat gatctggtaa atgaaaaatg
     4921 cttgtccaaa aaaatgtggt aaaatcagcg gcacatacgt cccaaccaaa ttcagccgat
     4981 tgtaaataat aaactgcgga atcaaaatca cttgtgcagg aatcatcatc gttactaaca
     5041 tgacgacgaa ccagaatctt tttcctttga aatcgattct cgataatgcg taagcaatac
     5101 aagtcgagct cagaactgtc cctatcgtcg aaactgcaga gatgacaaac gaattcctaa
     5161 agaaagtaaa aaatgaaatt ccagcaaacc ccgcccatcc ttttgcgtaa ttttcccatt
     5221 gaaaagtctc tggaatcaaa ttcagcgaac cactcataat ctcttgatcc gttttaaaag
     5281 aacctgcgat catccataag accggataaa tcatgactag acctaatagc aaaataaaac
     5341 cgtattgaaa ccatttcgtt actgtattat tcatttcttc ctcctctcat tcacacaacg
     5401 acccattttt ttgatgattt gaaaatcaga aaggtgacag ctcccataat caacagcatg
     5461 atccacgaaa tagcactagc ataccccata gaattaaaag aaaaagcttg gttatacaca
     5521 taaagagcat aaaagttcgt ggcatcattc ggaccacctt gggtaatgat aaatgcctgc
     5581 gtaaaagtca taaacgccgc gattgtctgc ataaccaagt tatacaaaat gatcggcgaa
     5641 agaagaggta acgtgatctt gtaaaacgtc tgccatttgt tcgctccatc gatctctgcc
     5701 gcttcataat acgagcctgg gatctctttg atacctgcag caaagatgat cattgccgaa
     5761 ccgaattgcc aagcagacat caaaacaaga gggatcatga ccagtttttg atcgctaaac
     5821 caggaaagcg caggtgcgcc taatcgttcc aggaaaatat tcactaaccc gtttttagaa
     5881 aacaactccc gccaaactag agcgatagaa atactgccac cgatcaaact aggcacataa
     5941 tacaaagaac gaaagaaagt cgttcctttg atattcctcg ttaaaagata agcaatcaat
     6001 agaccaaaga tcagtttcac aggtacagat gcaaatacat agagaaaagt gactcggatg
     6061 gagttcaaaa aacgggcatc actcagcatc tttatataat tatccaagcc gatccatgag
     6121 actggatttc ccaaacgata atcggtgaac gaataataaa gtgacaagat catcggtatc
     6181 actgttatca atagaaatcc taggatgaat ggagaagcaa atacgtaacc gaccacattg
     6241 tcttgatcca agaaccgttt tagtttttct tttttcatgc cttccttctg ctgttacttc
     6301 ttcataaatg actttttcaa ctaaaagatt gtattggtct tgaatttccg gattcaaagc
     6361 actatcaaag acattcactt cgccgttgtc catatcggaa atcatttcta aataatcaaa
     6421 tgtcgcttgc aaagttgcat catctgcacg agcgagctgt tctcgaatat tcgacatgat
     6481 tggtacacca cgttctcctt ttaaaatttc attggcttct tcactgtttt gaaagaaatc
     6541 aataaactta gcagcttctt ctttatgggc tgagcttttt gccatactca gcatttgcga
     6601 agaacgtaaa gggataccag attctccatc tgcttttctg cgaggtggat taatcaactg
     6661 aatctccata tcttcaggag cccctgacaa tatttctgtc aactggttac ttgatagaaa
     6721 caccattgcc gcttctctat ccctaaccgc ctgaaaagac agatccgtcg tacttgcagc
     6781 tccaccacct ggttctggat gggccccttt gtctaccaga tttttacgca gctggaaaaa
     6841 atctgttaga tagtctggat tttcaaatcc caagctgtta gatagatctt cccgattata
     6901 aaaattcaat cctttctcaa cttgtggtat ttgaataata cagccagcta taaaatcttc
     6961 gaatcttgaa atagcataga ttcctaattc ttcagtgatt tgatagctga tttgttcaaa
     7021 ttcatcccat gtccaatttg aagctggttc agggatattt aattgttcaa atatattttt
     7081 attgtaagca atcgcaaacg aattcacccc gttagacagt ccaactaatt gcccgtcgta
     7141 agtcgtcgtt gctaaatagg attcatctgc attagaaata tcaattatcc cttgctccac
     7201 atattcatct aatggttcaa tctgattgat gtattgagga aattgattgt tcagttggaa
     7261 aatgtcccag atatcatctg atgctgctag tgtacctaat tttgtgtaat atccttcata
     7321 atcataaaat tcataatcga tcgtcacatg gggatttttt tcttcataaa gctcgattac
     7381 ttttactgtt gcatcgtgtc ggacttgcga accccaccaa gccatgttca gcgtgatttt
     7441 tttgtcatct tcttgcccac aagccgtcaa gaagaataac ataacactta aagctaagat
     7501 gattctcatt cttttcttca ttctctgcac ctctacttcc attgttattt ttcaatatac
     7561 tgtattttta tacagaacat atttattaaa cgatcttttt caaggggaat aaaaaccaag
     7621 acttgcctta tcgttctgcc taggatgaac aactcaatac tttcaagaaa aattagatag
     7681 tgctgattct tctttcatcg ctaaaaacac ttcttgcttc aatgatggga ggataattac
     7741 gcaaagaaaa aaggatatca gacaactgcc caatatcctt gacatagcta aataagcatg
     7801 cttcttcgtg tgcgctcttc cctaaaaaac agaaaaataa ctagtaattc tgtatgaaat
     7861 taggtacaat atacacgtcg atgcacaagc attgactatg gcaacttgat tggtcagttc
     7921 ccgctgactt ttcaagcccc gacaatttag ctgctactaa attgtcgggt gcctttttta
     7981 gtttttattc gtttttcatt ctatagaaag cgcttcaata aatcaagaca tttttttgtt
     8041 agaatgctaa taataacaaa aggttttaac tcttcttatg tacattacag gtaaaaaaac
     8101 aaaaaaattt ctcttttttt tgaattctga acaaatagta ctggagtaca aaagacgagg
     8161 aagagccact atctgctctt cctcgtcttt tgattctcac ctgttattca tgattctttt
     8221 tctcagtttc ccgatatttt tccattttac gactaaaaag ctctccgcta cttggtggcg
     8281 tatatgtgat ggcatttgct cctgcttgaa tggtctcagc aatactttct tccgttggac
     8341 cacctgtcgc aatgatagga agttcaggat attttttacg aaaatggcgt actgcttctg
     8401 cagtttcttt acctgcactg atattgataa tcgaaatacc agctgctaac ttttcatcta
     8461 tttctgtata tttcgacgtt accgtactga taactggaat atcaactact cgacaaactt
     8521 cttttactgt ctctaaaggc gtaggaccgt tcaaaacgac ccctaacgaa ccttgcgctt
     8581 ctgcgaacat gctcatgtag gaagaacgag caccttgtgt caaaccacca cctacaccgg
     8641 agaaaactgg aatgtcagct gcctcaataa tgcttttagt aatcgctgga tgaggtgtaa
     8701 aaggatacac agcaattact gcattcgcat ttgtattacg tatgatcgca atatccgtag
     8761 taaagatgat cgaacggatc aattttccaa atatcttgat cccgcttgct tctaaaatga
     8821 tttccggtac tcttacgata tcttttctca attccgtcat gatttctggc gtaaattgtt
     8881 ctgccatcag ccacttctcc tttgctatct ccggtcatac tgattcaatt gatacgtctc
     8941 gatgacctgg atgatttttt gcgcataacc ggggtctgtc gcataacctg catcttgcaa
     9001 tgcttgtgcg gcttcctgat agttggtggc cgtaatcact ttttcataca gcgcaggatt
     9061 ccaatctact ccttggacga acagttgtgt atggtcatcc atggactgtt cccatgaatc
     9121 atacactttg aagtctcctt ggatagttat ccattcttcg ttaacaaatt cctttgtttc
     9181 caggcttact ttcttctgat ccccataagc cttgatcccg aaaagattat tgtatttgct
     9241 agccaaggta ctttttcccc aatttgattc taatatagct tgtcctaaaa tgatactagg
     9301 aagcactcca taaccatctt gtagttcttg ggcatgcgga acaagccgtt caataaattg
     9361 ctgatgggat tgcgcttcat tttcttgctg attatcagta aaaggagaag acagattctt
     9421 caaggaaaaa acaaaggcta agagtatcac tgctacaccg gcaaccactg ctggccaacg
     9481 gatattcttg cgcttttgct ttttccactt ttttttcgcc atgatcaatt cctacttttc
     9541 atttaattca ttcagatatt cttctagcgt caattggtgt ttggtgatat actcagcgtt
     9601 ttctttaccg acataacgta agtgccaagg ttcatatgtg atcttagtta tatcttcgtg
     9661 tccttttgga taacgaacga taaatccata ctgcgcagca ttatctgcga tccatttagc
     9721 tccaggctga tctccgtaac ttgcatccaa tacagtagaa ggataactgt tgtaccagtt
     9781 ttgatcgacg acatcgactg ccagacctgt gtggtgttcg ctgtatcctg gctccgtaat
     9841 cgtttcttta gtaatctttg tcgcctcctc ttctgataca ccttgacgac tcatcacatc
     9901 ttggacattt tgttcgaaaa cttgttgttg ggatgataca gaccggtaag ctgagaccat
     9961 cactaatgga aatccagctg cttcagcagc cttggcaaat gcttcatatt ctggagcaat
    10021 gcgtttgtcg atcaagtatc cgttacttaa tgatgccaat tgagtagact catcaatttc
    10081 tttttctaat ttgtgatctg gaccaaccaa aactagcttc caatcgtctg ttttaacatc
    10141 tggcaattgt ttggacgtac gatcttttgt ttctttgtcc tttaccttgc tgctggttgt
    10201 ttgactgttc ttttctttac tagcagttgt tttggtgctt gttcgttcga ctgcttgatt
    10261 gccggcttga acatgcgaat catcatcgct aaaaataaca tagcccattg ctgcgatcat
    10321 aatcacaact gctccaaatc ctaaaaattt attcacttga aaaatccttt cgttgttctt
    10381 cacgttcccc attcgcctca tgaacgtaag tttcttccag attttcattc acccaatcga
    10441 gaagttcgat tttttctcct tcgatctgtt tttgaacatc tgctggcaga cgaaaattca
    10501 aaatatcatc atagccccat tcatcatatt gaatacttac tttcaagtca aggtatctac
    10561 cagcctgctg attatttttc aaagggatca gctcgacttt tgctgctttg ctgctaagcc
    10621 atttttgcgc gatgcgtgtt ttgatttgct tgtactctgc aacggcaacg tttcgttttt
    10681 cgggaaaaat aatcgtttga cgcaaaatct tttgcgttag catccattca taatcagtga
    10741 aaagattttt tactttctgc atcgcttcag atggcagatt tttctcgcca tctttccatt
    10801 tttgccattg ttctggactg atcagcaagt attcatctaa aaaatttttt tcagtttcaa
    10861 actgttctaa taatacactc ataataagat caatatatac ttgatgcacg tttatcccct
    10921 ccttttaact attctactta agttttgatc aaaaagcacc tcttaaatcg ctgctttcat
    10981 tttttttaat gtttcaagtc ctctcctctt tattcttcgt gatttttgtt ataataatag
    11041 acagacaaga aactcaatcc aactatgaag gagcgttttc agatgaaaaa atatcaatgg
    11101 ggaatcgttg gtcttggtac catcgcacat gagtttgccg aaagcttcaa tcaagagacg
    11161 agcgaactaa cagcagttgc ttccagaact tcagaaaaag cggaaaactt cgctcaccgt
    11221 tacaatattc caaaagctta cggaagctat caagaaatgt tagacgatgc tgaaatcgac
    11281 atcgtgtaca ttgctgtacc aaatagacag catatcgacc atatcttggc agcattggaa
    11341 gcaggcaaac acgttctatg tgaaaaagcc atcacgatga ataaaaaaga actggctgac
    11401 gctatgcgct tagcagaaga aaagaatttg atcttagcag aagcgatgac aatcttcaac
    11461 atgccacttt atcagcagtt acgctcaata atggatacag gaaaactggg ggctttaaaa
    11521 atgatccaag cacctttcgg cagttacaaa gaaccagacc ctaaaaaccg gttcttcaat
    11581 ccagaattag ctggaggagc tctgttggat atcggtacgt atgctgtatc tttcgctcgt
    11641 ttctttttga gctctcaacc tgaagtagtt gcctctacaa tggttccttt tgaaacaggc
    11701 gtggacgaac aatccgtcac tatcttgcgt aacaaagaaa atgaacttgc tgctgtcagc
    11761 ttgacttttc aggcaaaaat gccaaaagtc ggtatcgttg cctttgagaa cggttatgtg
    11821 acgattgctg attacccgcg tgctgatcgt gcggagatct tatttacaga tggtacaaag
    11881 gaattcatcg aaagcgggaa tacatctgaa gcaatgaatt acgaaataaa gaatatggta
    11941 cacatgattg aaggtgactt gcccaatcgc tctctcttct tgacaaaaga tgtcatcgaa
    12001 atcttggatc aaatgcaagt tctatggaaa aatatgtaat tttaaaaggt tgtgaaaaag
    12061 ctgttctgca tcaagtaata agaacagcca aacaaaaata gctcctccta tttttcgttt
    12121 ggctggactt attaccgcag aggaaagctt ttgaacaccg tttaagaaag aggttgtgag
    12181 cctaccgttc agctccgagt cacaaggagc aatttcaaaa aacagcttct catgtttgtt
    12241 gaatattgtg acttgtgacc gaggagttga caggagaaca ccgtttaaag aagaggttgt
    12301 gaaatcagct ttttgacctg aatattagct gaattcacaa cctctatttt tggatttttt
    12361 gttgggagaa ttctgccttg atttcaataa aacttttaaa agactgcaaa aactggaaca
    12421 attctgctcg atcctcgaat tcagggcgac tagtcggcag aggcatttgc cgatacaata
    12481 cataaacttc ttcaatacgt gttaaaattt gtctgccatc atttgactct gaaaatgttt
    12541 ctgcagtata aatcaataaa ccatagatat gttttccata ataagctggt tgctggattc
    12601 tctctagatt ttccagcatg tctttaatca cattggcctg tgctcttctc atagagaagt
    12661 aagtttcgta gtatagcggc tgtctgagcc agtagttttc ttgatgttct ctggcatctt
    12721 cttggctttc gcgaatgaat gtcagtaaat gttcgcattt gatctgcaca tcttctttat
    12781 tttctgataa aagaaattct gccatttgtc tcaaaatatt tcgaaattct ttttcgatct
    12841 gtatttggtt ttccttcagc tgcttcgtac gatctggcat gtaaacattt gcaagcaaag
    12901 caaatactaa tccaatcgcc attaatcccg cttcattgcc gatcagctgc caagacatgt
    12961 tttcctccac taaataatga gtgaccaaaa ccgaatttac tactatccct tcggttaatt
    13021 tgaagcgaga ggcaagcggg atgaacagga ataaaaaaag actgaagcca aaaatagtat
    13081 acccaacagc aaaaaaactg aacagtgctg ttgccgtagc taatataaag gctgcgattc
    13141 gattcatgcc cgtacgcaat gtagagcgct tggtatttcc tacacttaat aatgcaatga
    13201 tccctgcagc tggccaatac aacagcgata agctacccgc tgtgaccatc gaaaagaaga
    13261 cactgatcac tgttttgact gttcgcattc ctatacgcat ttacttcccc cttttttatt
    13321 ttaattatac ttgaataatg aaaaactagc tatcagacaa aagtttaggt atgatagtaa
    13381 aaagaccaaa aaggagaatc tctatgcaat ttggccgttt tcgactaggt atgcgtacga
    13441 tcaaaagtgc cttagctgtt tttttatgta tcttgttttt tcatttgact caacgaggaa
    13501 tcccgatgat tgctgcatta tctgctgttt tttctttgcg tcaggacttg actacaaccg
    13561 tttcttttgg aaagtctagg atcatcggca atacattagg tggtacatta gctatcattt
    13621 atttttttgt caaaagctat tttcacaatg attttttggt agaattgatc ttgcttccta
    13681 ttctagttat tattgtgatc gtcgtgtcag atggtattaa caataattcc ggtatcattt
    13741 ccgcaatagc tacgttgttg ctgattgcgt tgagcatccc tcaaggcgaa tccgctttgt
    13801 atgctatcca acgtgtacta gatactttta tagggacatt cattgcaatc ggtatcaatt
    13861 tctttcttcg tcctcctgaa acagaaaaga aggaagaact atcagaagat ttggtcgaac
    13921 tccaaaagaa agaacgattg ctaagaacaa atttacaaga gatagaagct aagatccaaa
    13981 aacaaaagaa aaatagcgag gactaatttt cttttgcaat aaaataaaaa aatggagaga
    14041 agaactttaa aaagtcttcc tccatttttg attgaacggt gaagtgtact actttattct
    14101 tccttcatcg agttttgttc tgcctctttt ggcgcttcat acatatttac ttggctagta
    14161 gtcccacgtg tcgacaaatt ctctacgatt tccttcaagt caagtcctgt tgtcgctttt
    14221 agtgtttctt gagtagaaga aagcaaattt gtggcataat ttgtcactcg gttcgctcca
    14281 ctggtttctc ctgaactgct tcctgtatcg acaacagaga ttttttcgat atttcctagc
    14341 ggctgtgctg cttcgcgcat caattgcggt agcatatcga taaccatact caagacagct
    14401 gcttcgccat attccttgaa ggcattggcg atcttttgtt ttgcttcggc ttctgctttt
    14461 ccttttgcta gtgctgcttc ggcttctgct tgtccagcta aacgtgtctt attggcttcc
    14521 gcttctgcca gtgcttccac acggaatcgt tctgcttcgg cttcagtcac ttctttgact
    14581 ttttgagctt gtgcttcttg ttctttagcg taccgatcgg catctgcttt tttcttcact
    14641 tccgaatcat attgcttttc acgacgtgtg atttcttttt cttccagttc gatttgtttt
    14701 tggcgttcta cgattttaat ctgcatttcc tgttcgatta cttgttgttg tgctcgggca
    14761 ctttctaaat tgtaggcttg gtctgctttt gctttagcaa catcttgttc ttgtttgtag
    14821 gttgctaatt tcagttcttt ttcttttaat gattctgcga tctctgtttg tcgctgtaat
    14881 tcagctgctt gtgattcttt ttctgcttga gcttttttga tacgcgtttc ttttagagct
    14941 tccgcttctg ctatatctgc atctcttttg acttgggcga ttcttggttt ccctaaagag
    15001 tcaagataac cgtttttgtc tctgacctct ttgatagtga aagaaacgat gatcaacccc
    15061 atttttgcaa ggtctacaga agcgacttct tgtacgcttt gactgaactt atcgcgattt
    15121 tgatagattt cttctacagt catagaacca agaatcgagc gcaagtgtcc ttctaatact
    15181 tctcttgctt cattttccag ttcttctctt gtcttgccta aaaactgttc tgctgctgta
    15241 gcaatctctt ctacagatga accgattttg atgatcgaag taccatcaca catcaccggt
    15301 acgccttgtt cggtatacac ttcaggggtg gagacgtcca atttactcga taatagactg
    15361 atccgattcg aacgttgaaa aactggcaag acgaatgcgc cacctccacg cacaattttg
    15421 attttgttat tgctttcatc aatgtgcaca tttttgcttc ctaaataact tccactgatg
    15481 atcaacgctt catccggttt tgctgtctgg tacttcgcta caaagacgat cagcaacatc
    15541 agtaaaatca aagctgcgat tgcgatcgcg ataaaaacaa ctggtatttc catttaacac
    15601 acgctccttt aagcttttat catgctttca taaggcatga cataacagat tctttccttg
    15661 atctcaatga tcaatacttt cgttccccgc tcagctgtct gttcttgggg gctgtaaaaa
    15721 caagctggac gactaatgga accggtaaca cttttcaact gaatttctcc catacctcgt
    15781 acagggatcg gtgtgatcac aacagctact tgtccttcca gagttttttc agaagacgac
    15841 atcgttgatt cggcatgttt catcggcatc aatatataga aattaagcaa aaataccaac
    15901 actatcgata tgcctccact agcaataaat aaaaataaag atgattgatc tgttaaacgc
    15961 tcacctagaa aaccaaataa gcttgtgaaa gttaaccaag ggataataag catcggatca
    16021 agcgggccat caaaatcgaa tacatctcca aaaataatca ataggactgc caatcctgca
    16081 caaatcagca atgtatacca ataaatcgtc tcaagtgcga ttccccctat catatacacg
    16141 cctccttcca tttctacgtc tatcatacca atgtttgaaa attctgtata gcttctttta
    16201 ccgactcagt ggtgtttttt ataggaaggt tgtgtttatc gttgacaatt tgttaaattt
    16261 ttgttttcat gttaaaattt tcacaataaa ttctttcgag gtagcctatg tttccttttt
    16321 ttaagaaaaa accattaaag ctccagaaca cgatcatcct tgtcgtttgt ttctccacat
    16381 tcgtttcttt gattgtttct gccatattga tcaggaattt tattctttcg atgaaccgat
    16441 ctagtgtgtc agataaaatc tctagcattg ctaaaatcgt tgccaatgat aaactcatcc
    16501 aaaaaaagct gcagactggt gacccgacac atgcgattca aggaataaca aatgaaatca
    16561 tgattgcatc gaatttagat tttatcgttg tcatggaccc tgaaggcatt cgatattctc
    16621 acgcagaccc aagttacatc ggtcttcctt tctcaagtca tgaagatgtg aaaaaagtga
    16681 ttggaggaca agaatttttc tctgaaaaag aaggaatgtt agggttcggc ttccgctatt
    16741 tcgtccctat ttattatgat caagagatta ttggtgcagt ttgtgttggc ttaacaaaca
    16801 acacgattga caatcaagtc aaagatggac aaaaaatgat tgttttttca ttgattattg
    16861 gacttagcgt aggcatcata ggcgccgttt ttcttgccca aaaaatgaaa aaagtactgc
    16921 ttggacttga accacaagaa atagcagaac agttaagtga aaaaacgatt atcgaaaatg
    16981 aagtagatga aggaatctta gccatctctt ctgataaaac tatcctcttg atcaatacac
    17041 ctgcaaaaaa cattttctac caaacagagc agctattgga tttcgaagta ggaaaaaaat
    17101 cagtgataat ctatatgacg ttttgtttaa agagacgatt gagcatcaaa cgaccgttcg
    17161 gactcaatcc atgtatttaa atgggataga aactatttct agcgttacac ctctttttag
    17221 caatgaccgt tttttaggag cagtcgcaac tttacgaaat cgatcagaag taatgaaatt
    17281 aatggaggag cttgggggga cgaaacaata tataaactcc atgagagctc aaacccatga
    17341 attcatgaat aaacttcatg tcatttctgg tttgattgat ttgaagaagt atgaagaagt
    17401 cacccatttt atccagcagc taaatcaaaa ttataaaaaa gagattggcc aagtctcaag
    17461 tctgatcaaa gtacctgcaa ttgccggttt tttattaggc aaaataaacg aagccaaaga
    17521 acaatctgtt gaaatgctgt tagaaagcga ttcttttatc cctaaaatgg agatggatga
    17581 ctccatccat tcacttttgc aagtgttagg gaacttactt gataatgcaa aagaagccgt
    17641 gctacaaaaa catgaaagaa acggacaggt ccggattcat ttaaactatg acacagaagc
    17701 tgaagtattc atcgtgaccg tctgcgatgg cggtgtcggg atatcagaag aaatctcaga
    17761 aaaaatattt acgctaggtt tttcgacgaa gggaatcaat cgtgggtttg gattgaatct
    17821 catccattca atcgtcaaac gccacggtgg atttttagaa ttttccaaac aaacaaatgg
    17881 cggtacaatc gtctatctag aattaccaaa aaaggaagat caaaatgatg acaaatgtcc
    17941 tgataatcga agatgatcca atggttgcag aattgaatcg tacttttgtg gaaagcatcc
    18001 ctaatatgaa cgtaatcgaa agtttcagaa atgtagaaga aggaaaaaaa tatttcagac
    18061 aaaaaaacgc agatctgctg ctgcttgatg tatacatgcc tggcgaaagt ggtatccatt
    18121 ttttatcttg gcttagacag caaggaaatg atatcccagt gattttgatc actgctgcag
    18181 atgatatcga aaccataaag aatgtgatcc gctacggtgc cttagattat ttgatcaaac
    18241 cgttcacttt gcaacgtttt cagctggcga tcgaaaagta tcttgctttc tctcagacaa
    18301 tccaaacgac tgaaaaagcc gatcagaaat tgatcgatca agtattcaac cacaccactc
    18361 ccattcacga aataccatca ttcatcgatt tgcctaaagg tctgtcaaaa ctgactttaa
    18421 aaaaaataag tgcatttatt ctaaaagaag atactatttt ttcaacaaaa gaactggctc
    18481 aaaaaagctc actgtcacgt atatccgtca agaaatattt gaatttttta gtagaacaag
    18541 atatattgaa cgaaagaatc aaatatttgg aaatcggcca gccgctaacc tgttatgaat
    18601 tgacaaagaa tgggcggaaa aagctcacag attactgttg agtcatcgaa ttatcccata
    18661 caaaatagtc caagtataca gaagtaacaa ctgtttactt ggactatttt taaacatttt
    18721 attgacgaat atccatgttt tcttatctta attgacgggc tttaggaaga gatacctcat
    18781 ttttcgtttt tccagtttct attatttcac gaaagttctc gaaacttgtc gaaatcatat
    18841 ttgataatgc ctcatctgta tttgaaccta catgcggggt tacaagaact ctagggtaca
    18901 tttctactaa ttgctggata gcagggtctg gaatcgtctc ccatggcttg aaggatcgga
    18961 aaaaaacctc tgtttcattt gcaaaaacat cggttgcaaa accagctaaa tgattgcttt
    19021 tcaaagctct taaaatagct tcattatctt gaagctctcc acgggctgta ttaatcaaga
    19081 tgctaccttt tttcatttga gctaaaaatc cttcattgat catttgatcg ttttcacctg
    19141 ggatatgagg tacatgaata ctgacaatat cgcttttggc taataactct tcaagcgagt
    19201 ggaacgtcaa tactttttta gcagcttctg tctgatgaat atcgtagccg atcactttgg
    19261 ctcctagtcc cttaaacaaa acagcttccg tcaaccctat cctgcctgtt cctatgatcc
    19321 ctactgtgca atttctgatt tcacgactaa acatttgctc atccactcga aaatctttga
    19381 aagatgttcg gatcgtcgta tacgcagtat ttctcaaaag catcatggca aaagtcaaag
    19441 ataactctgc tatcgcattt ggcgaatacg atggtacacg ggctactgtc ataccaaaat
    19501 cagctgccgc ttgtagatcg atatgattga ctcccacagt ccgtgtaaaa acataacgaa
    19561 tcccatattc ctgcattttt gcaagattgg tctcatctgc tatacaattt cccctcaaca
    19621 atacagcatc atggtccttt gctgacacga tattgtcatg tgtcaacagt tcttcataaa
    19681 gactgagatc ataatggtaa atattcaatt tttcaaaaaa atctatctcg ttcggacgaa
    19741 caccgtaaca tgcgattctc atactttctt cctccatttc ttatgaaaat aaacctatgc
    19801 catgaatcat ttgtaataca acgatccata caggtgtcag taccacagca aaaatagttg
    19861 acagtaagga tgcattggag gctaaaagtg cctccctatc aaaactgata gcataggctg
    19921 ctgccactgt tgcagctggt gtagccatca tgataacgat tgttgccagt gcttcaaaat
    19981 tgactggtag aatattcgtt gccgtcaaaa ctgctagaat cacgatattc aatgcaggta
    20041 caaataccac tttgactaga ctgtaatacc atgaagtttt ttcagatgca gcttttttga
    20101 agcttacttc acctaatgtt gcgccgattg ctagccacgc caatggtgaa cttaaaccag
    20161 ctaaataatc catcggctga aatagccacg gtgcagtctg gtcaattcgc aaaaacgcta
    20221 cttggcttat ctctccttct gcacctgtaa ctgacacttg tggcagatac ccttggaaca
    20281 gccaaatgaa aaggccggca aaagtagcga taatgattgg attgacgaac atcgctttga
    20341 tatttttagc ttccatcttc aagccactca tttttatgta tccataagaa tacaaaaaga
    20401 ttcggtagcc aatattgaaa attgaggaat acatcacgcc gacaggacca taaatcgcac
    20461 tgacgatagg cgttccgaaa aaggtcgtag aaccaaagat cgtcaggaca cgcaacgttg
    20521 tttgcttatc ccctttatag cttaagaaaa aaggttttga aactagaata agaataatat
    20581 aaataactaa tccccaaatc aagacattga ttccttgcat cagtaattct gtattgatat
    20641 cttgcatgaa agctcgaaat gccagtgctg gcaaagccac tgttaagacg actttcgata
    20701 gaatttttcc aacagattca gaaaaaatcc ccttttttct acaaacaaac cctaataata
    20761 taataaaaac tgttgaagtg attgcactga caatcgacat atctgtcaaa gtcgatcgaa
    20821 aaatgtcaaa taagttcatc tttctcctcc taattatgtt catttattca caataaaaaa
    20881 gcttttattt tgctaatacg tattaacatt tttaatttta tctttacatt gttacttatt
    20941 gaacaatagg aaattaaaga aacttttgaa acttatgtaa agtgattttt actagaacac
    21001 aaaaaaacct taaaaccaaa cttaagaagt ttagttttaa ggttaactta aattatatta
    21061 tgcgtggaaa cgttccacgc catctagata agttgcttcc aatgtcatat cagggttcaa
    21121 tacgataaag tctgcatcgc gtccttgagc gatttttcca catgtatcat cgattttaca
    21181 gctgattgct ggaatcagac tagccatcat gactgcttgt tctggtgttg ctaatcccca
    21241 gtctacaaca tttttaattg cttctttcaa tttcaatata cttccagcta agttgccaga
    21301 ttctagacgt gctgttcctt cttttactac tactggaaat tcgcctaaat tgtaatttcc
    21361 atcaggcatc ccaccggcca tcatacagtc tgtaattaat gcgacatgat catgtcctgc
    21421 tttttccatt aggattcccg cagcatttgg atgtacatga tggccatcac aaatcaattc
    21481 agaaaagaca tgttctaaag ataataacgc acccaccata cctggttcgc gatgattcaa
    21541 tccacgcata ccattgaaag catggacaaa tacacttgcg cctgcttcaa cagctttttg
    21601 cgcttcttct agtgtagcat tactgtgtcc tagagaaaca actactcctt cttcagtcac
    21661 ttgtttgaca aactcttcta caccttgacg ttccggtgct aaagcaattt ttttgattaa
    21721 accacctgaa gcttcttgcc attcatggaa agtatccaaa tcaggatccc cgaaataact
    21781 tgggttttgt gcccctttgt gttcttctgt gaagaaagga ccttcaaaat agattccttt
    21841 gattttagct cctggtgctt cctgatacat tttgccgatt gtctcagcta catcttttaa
    21901 acgttctttg cttgacgtca atgttgttgg taaaaatgaa gtcactccac aagataacag
    21961 accttcagac atcactttga tgccttctgc atcattatcc attacatcat gattcatata
    22021 gccgtgaata tgtgtatcta ccaaaccagg agcaatccat ttgccgcttt ggtcaatgat
    22081 gttgacttct ccctcaggta gttctttggt gtattcacca aaaagtccat cggtaatttc
    22141 aagatagcct gcatgtttga cacctgacgc taaaaagaat ttgtcagcat agataaaagt
    22201 tctcattcta ttctctccct ttgtttttct acctctaaag tactcttatt tagagaagaa
    22261 gtcaagaatg gtctacacca tttacaaatt ttttgaaatt tgtttcacag cttgtgctaa
    22321 gccttcatag aatgctgctc tttctggttc ttctttacgc ttgtatatta agcttttaag
    22381 cacaaataaa tcattgaggt aatagtagct attcttctct tgagcccaat agattccttg
    22441 attcacctgt tctaacgctt cgtcgatttc attttgatgg aataagaaat ttccataatt
    22501 ataaaaaatt tgcgaaaatt cactttttga tcgttcatca gctgattgat gcagcagttg
    22561 gatgcttcga ctcaaatagt agcgtgcttc agccgttttt cctgccacac catatatttt
    22621 tccgatacaa ctaatcagtt gtatttctgt gtcagaaaca tggattcttt ctttttggct
    22681 agagtagctt aatgcttctt tcaaataata aagggactga gagatatttt gacgcaacgt
    22741 gaattcgcaa acaccgagat aataataata gcgttgaaag tcagttgctt gatacagatt
    22801 ttcagaaata tcagaagatt ttagtacttg ttctaacttc cgataatcac gtttttggaa
    22861 acaactatcc aacagttcaa ataatttatc gtttgtccga gtagtttttc gagagagatt
    22921 catcacatga tcaatcgtta cccctagccg ttgacaaatc tgcatcatca ccaatacatt
    22981 cggcagttcc tcattatttt cgatccgact taatacactt tgtgaacaaa tatcatgcga
    23041 aagcattttt tgcgtcattt tttgttcttt tcgaatctct ttaatgatcg ttccaaagga
    23101 ctcgattaac gtttccataa gtatcctccc caaaatatgc gtattcgcat tctattcgta
    23161 ttttacttta ggaagtcgca aacttcaaga aaagacttgc aactaaataa tatactttag
    23221 ttatttaaga aaactccact agaaagagag cctcagtgtg tttgtctgtg gccctcctac
    23281 tgaaaaattt ggtttttaaa atcatttttg gcgataggtt gctaggaatt ctctcatttt
    23341 cttagctgtt tgtttcaaat catccatttt aggaagataa acgattcgaa aatgatctgg
    23401 ctggttccaa ttaaatccac caccatgtac aagtagaata tgatgttcat gtaaaaagtc
    23461 caaaacgaat ttttcatcat ccaaaatatt gaatcgcttt gtatcaattt ttgggaatat
    23521 ataaaaagca gcttttggtt ttacggctga aagaccagga atatcattga ttgcattata
    23581 gatatattct ctttgttcat agatccttcc gcctggcagg agcagctcat cgacactttg
    23641 ataaccgcct agcgcggttt ggataatttg ctgagacaat acattggaac acaaacgcat
    23701 ggaggacaac atattcagcc cttctatata gcctttgacg tgtgacttat ctccacttag
    23761 gaccatccag cctactcgaa atcctgctac acgatgagat ttggatagac cgttcagcgt
    23821 aacaacgaac aaatcaggcg ctaatgtagc aatcggaata tgttctagac catccatcac
    23881 taagcgatca tagatttcgt cggaataaat aatcaggtta ttttgtcgag ctacttcaac
    23941 gatttcttct aataattctt ttggataaag tgccccagta ggattattag gattaatgat
    24001 aacaatagct tttgtccgac tcgttatttt tgatttgata tcatcaatat caggatacca
    24061 ttctgcctct tcatcgcaaa tataatgaac aggtgtgcct ccagctaatg caatagaagc
    24121 cgtccacaac ggatagtccg gcattggaac cagtacttca tctccattgt ccagcaaccc
    24181 ctgcatacac atcgtgatca attcgctcac tccgttgccg gtgtagatat cattgatcgt
    24241 cacattcgga aaatgtttca gttggcaata ttgctcaatt gcttttctag cagaaaagat
    24301 ccccttggaa tctgagtagc cttctgagtc acgggcattc atgatcaaat cacgaatgac
    24361 ttcgtttggt gcttcaaatc caaaaggtgc gggatttccc gtgttcaatt ttaaaatact
    24421 aatgccctct tcttgcatcc gctccgcttc ttctagaacg ggaccgcgaa catcgtaact
    24481 gacaccttct aatttgttgg atttttcaaa ttctctcatc acttagccaa tccctcattt
    24541 caaaaagtta gaactttcca aattttttgt tatttactat ttcaatatca tactccatcc
    24601 tagaaaattt tgccacttat ttaggaaaaa cgtaagtatt aaaaaaacac aaacaagcgt
    24661 tgagaactta attgtcatga tagtcattcc atagattcgc tgcccaatcc attgcaattc
    24721 cacaaatgat tcctagcata aaaccaagca aaaaacgtcc gaatgcaagt ccaatcaata
    24781 cacctaagat cattcccagt cctgaaaatt ctccgtcata tttctccatc ttaataagtc
    24841 ctcccatttt tgagcttgac aactatgtaa aacctcgcta tcattagtgt gttattatta
    24901 cgcagatgtg ttggaattgg cagacaagca tgattgaggg tcatgtgagc gttagctcgt
    24961 gtgggttcga gtcccatcat ctgcactaca actcgtgaag ttaattttgc ttcacgagtt
    25021 tttttgtttg aacgtttctt tatgccatta tatacatatc actgtatcct ttacaaaaag
    25081 aacgtacaat catgtaaaca gcctgaggtg agtatatgaa ggtcggaaac aagctactcg
    25141 tagaaacttg caagtgttaa aacaattaat cgaaaaacga gtaaaatccc tcacctaata
    25201 ctaaagcgag ggattttttg tatattatct gtttatcacc gttattataa aggtacaaat
    25261 accaagcacg gttaatccaa attgcataaa ttcatttttt tgtacactca tgcttttcat
    25321 tagctcttta actgccatta gtactaagat ggcgataaca acataaagta tatagtacgt
    25381 ccgcattccg gaagcccata ctgtgggaga aaatcccata attaaccttg aacaaaatcc
    25441 cattatcatc aaaataacta aaaaaataga agttttctta ttgtcaaaac ttaaataaat
    25501 tccaacaatt aaacatagta ataatgccaa gactactaaa gtagctatcc aagtacctgg
    25561 ataaaaaagt gacagtttcg ttcctaactc tgtaaagaga ttattcaggt tactactact
    25621 ccaaatcatt gcccttttat ttccattgac gtaagtaaag ctttgaccca ttgtattacc
    25681 aaaataaata atcaggttta agaaaaacgg taatgcagtc aatatacgca cataatagtt
    25741 ttgacatttc ctatatgtta gaaggaaaat taagaaaaat agcaataaaa ataagatatt
    25801 catgtctaaa aaaaacggct ttccaaatga tgaaatacct aaatctaact tattaagaaa
    25861 gttgaaattt ttatattcag gaaaccattt attaatttca tgtgcagcac gtaatgcatt
    25921 ccctggagtt gtcaaagaga aaattaattc tgctaagctt attattgaaa atactgataa
    25981 cttataatta tatcgtccat tgacaatcaa atataaacta actatacttg ttagaacaaa
    26041 aaaacaggcg ttgacttgtt cttgattagt taaaaatatc aataaaggaa tacttaccca
    26101 atatattttt ctatttattt cttctcctct taagagttga aaaaacggat aaaaagttag
    26161 taaactaaat gctactggcc actgatagtt caaggtggtt gctctccaac cggtttcccc
    26221 cattactgtc aatggtatta aacagtatat agagcaaacc aataataaat ttttaacatt
    26281 tattttctca aaaacatact tgcaaagtaa tactacaatt cctaacataa tcgtagaatt
    26341 taataatttc catagcataa agtgtttaga aaagaacatt aagaatagtt ctataataat
    26401 tcttgaactc catgtcttat aatgttctat cgaagcaccc aaatagccaa gttcatctaa
    26461 tctacttgca taaactacgt catccgctct catagtcggc atatacatat taaagagtaa
    26521 aaagataccc caaatcccta ttagtaacaa gatcagttct ttgttattcg ttatttttct
    26581 attatgtttg ttcattttta tactccttct tatatttcct aatagagtat aacatagctt
    26641 taagcattac ttaaagataa cttaaaagtt atcttttaaa tacttaattg ttaaatacct
    26701 tatttgatgc aaaagaaatc tacgatagtc aacaaaaaaa ttttcattat atccagaaaa
    26761 atttttctta gttaaggacc tatggattgc agtaatgcag aattcgtcga ataaactgcc
    26821 aaagacatac aatcatattg cttttaaaat tgacgaacaa gatatagatt cgtttgtctc
    26881 aaaaatccaa atgttaggtt taactgtcga accaggacgt tctcgtgtga aaggcgaggc
    26941 ttcttcaatt tacttttacg actatgataa tcacctcttc gagttacata ccggtactct
    27001 ccaggaacgt ttaaaatcct acaattccac tacctgagac tagtaatgac tagtctcttt
    27061 ttatggcctc tgatcccccc attgagcgaa tactaatagg gcttcgaatt gatctgttca
    27121 atctttaagc aatatccctc aaatgagttt ggaaagttta gtgattcatc acaaatactt
    27181 aaatacatgt agttatatat agattgaggt tcttgtgagc gtatgctcgt gtgggttcaa
    27241 atcccatcgt ctgcatccat ctttccaaaa caagcggcta ggacattttt gtctcagccg
    27301 cttgttttct agtttattct ttcatcttgt cggacctttt ttcttgggta tagacttttc
    27361 tcatattcta ccacaatttt gggtttccct aacacatgat aggatctcac aacaaattgc
    27421 tttctattat tataataacc tgtaacgact aacttcatcc cttcgtctac ttcagctagg
    27481 aagttcaaac tatgggaagc aatcaaacaa ttgaccccat ttaaactgaa acggatcaac
    27541 ggtctggcac taacttgcaa agtacggatc ttatctactg ttccatattt tttctccata
    27601 accttcacct caataccatt atacgaacat acattccctt tgtaaagaag aaactctgtt
    27661 ttatcttttt gactttttgt ctattacctt tcttaaaatc atattggcac ttcctgcgaa
    27721 gatgaaggac atgtactaaa aaaaagaggc agcagtatag attactgttg cttctttact
    27781 aatttaggtt tacttgttaa gaaagattat ttacgcaaat gttcttcaac aatttgctga
    27841 gatttatcag ctgtatcttc cacttcatct tttgcttttt ctaacttttg aatatattgt
    27901 ttcaagcttg agttttcagc agttgcttcg tctaatttct tttgtgtatc tgttaactga
    27961 gtttctacat ttgttttttc ttgtgtcagt gttgcgattt gttgattcaa accatttaca
    28021 gtattttgta attctgaaag ttttgcttgt gcttcttgta gtcttgctgt ccaatctgca
    28081 ttcagatgat tgatttcatc atttttttgg ttgatctcat ctagtttgac attgatttca
    28141 ttttgtttgc tttgtagttg tgcatccttg ctgactaatt gactttctaa gttttgtttt
    28201 tctgctgccg attgattttt ttggttttct aattcattta cttttgtttg gtattcttgg
    28261 acatctgcct ttgaatcttg caatgtctgt tctttttgtg ctagtcgagc ggtaagatct
    28321 gtaagcgctt gctctttatc tgtcaatgta tgatcaagct gtgtcagatt ctcattgatt
    28381 gtatcaatat ctgtctctcc accccacaaa ataatttcat ttgcgtaccg cgtgcctgtg
    28441 tagccaatta gacatagaaa tgcaacgaca atcccaccag taataaattt ctttttcatt
    28501 tgcatagctc cttaatccaa tacttttaca tgctaaaagt aagtgatctc aaacacaatg
    28561 tcaagcgttt ttaaattaca tgtgtaattt taatataaat actatcaaat actcatttaa
    28621 tcctcctttt ttatctattt aaaaaatctt ttttatagag aaaaatgcag gttaatactt
    28681 tcttaaataa atatttatgg tgaacaaaaa caaaaaagca agttttatta aacgtaaccc
    28741 ttttcatttc atagattact gtatttatta caaaaaacg
//
LOCUS       DARBEC010000026        28456 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.026, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000026 DARBEC010000000
VERSION     DARBEC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28456
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.026"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            137..625
                     /gene="purE"
                     /locus_tag="SCL80_001114"
     CDS             137..625
                     /gene="purE"
                     /locus_tag="SCL80_001114"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEG1106996.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            618..1742
                     /gene="purK"
                     /locus_tag="SCL80_001115"
     CDS             618..1742
                     /gene="purK"
                     /locus_tag="SCL80_001115"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEG1106997.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            1755..3050
                     /gene="purB"
                     /locus_tag="SCL80_001116"
     CDS             1755..3050
                     /gene="purB"
                     /locus_tag="SCL80_001116"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEG1106998.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(3230..3607)
                     /locus_tag="SCL80_001117"
     CDS             complement(3230..3607)
                     /locus_tag="SCL80_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1106999.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            3863..4591
                     /locus_tag="SCL80_001118"
     CDS             3863..4591
                     /locus_tag="SCL80_001118"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEG1107000.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            4591..4845
                     /gene="purS"
                     /locus_tag="SCL80_001119"
     CDS             4591..4845
                     /gene="purS"
                     /locus_tag="SCL80_001119"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEG1107001.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            4847..5518
                     /gene="purQ"
                     /locus_tag="SCL80_001120"
     CDS             4847..5518
                     /gene="purQ"
                     /locus_tag="SCL80_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEG1107002.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            5519..7741
                     /gene="purL"
                     /locus_tag="SCL80_001121"
     CDS             5519..7741
                     /gene="purL"
                     /locus_tag="SCL80_001121"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEG1107003.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            7726..9165
                     /locus_tag="SCL80_001122"
     CDS             7726..9165
                     /locus_tag="SCL80_001122"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEG1107004.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            9188..10240
                     /locus_tag="SCL80_001123"
     CDS             9188..10240
                     /locus_tag="SCL80_001123"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEG1107005.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVEESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            10237..10815
                     /gene="purN"
                     /locus_tag="SCL80_001124"
     CDS             10237..10815
                     /gene="purN"
                     /locus_tag="SCL80_001124"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEG1107006.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            10812..12347
                     /gene="purH"
                     /locus_tag="SCL80_001125"
     CDS             10812..12347
                     /gene="purH"
                     /locus_tag="SCL80_001125"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEG1107007.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            12363..13637
                     /gene="purD"
                     /locus_tag="SCL80_001126"
     CDS             12363..13637
                     /gene="purD"
                     /locus_tag="SCL80_001126"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEG1107008.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            13759..14673
                     /locus_tag="SCL80_001127"
     CDS             13759..14673
                     /locus_tag="SCL80_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1107009.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(14806..16371)
                     /locus_tag="SCL80_001128"
     CDS             complement(14806..16371)
                     /locus_tag="SCL80_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1107010.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(16364..17815)
                     /locus_tag="SCL80_001129"
     CDS             complement(16364..17815)
                     /locus_tag="SCL80_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1107011.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITA
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(17829..18845)
                     /locus_tag="SCL80_001130"
     CDS             complement(17829..18845)
                     /locus_tag="SCL80_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1107012.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            19065..20153
                     /locus_tag="SCL80_001131"
     CDS             19065..20153
                     /locus_tag="SCL80_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEG1107013.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            20207..21733
                     /locus_tag="SCL80_001132"
     CDS             20207..21733
                     /locus_tag="SCL80_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEG1107014.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            21735..22940
                     /locus_tag="SCL80_001133"
     CDS             21735..22940
                     /locus_tag="SCL80_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107015.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            23197..24174
                     /gene="guaC"
                     /locus_tag="SCL80_001134"
     CDS             23197..24174
                     /gene="guaC"
                     /locus_tag="SCL80_001134"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEG1107016.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            24438..25862
                     /locus_tag="SCL80_001135"
     CDS             24438..25862
                     /locus_tag="SCL80_001135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1107017.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            26323..27432
                     /gene="pdhA"
                     /locus_tag="SCL80_001136"
     CDS             26323..27432
                     /gene="pdhA"
                     /locus_tag="SCL80_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEG1107018.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            27435..28412
                     /locus_tag="SCL80_001137"
     CDS             27435..28412
                     /locus_tag="SCL80_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEG1107019.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT         9197 a         4865 c         6426 g         7968 t
ORIGIN      
        1 tttgtcacat tccttttttt catctttaat aaacacgaac aataaaatgt ttttctttgt
       61 taacattcga aatttctttg acatcaaaaa aagtgattgt taaaatatga atgtagaaaa
      121 actgggagga aaaacaatga cagcaaaaat ctctgtgatt atggggagta cttcagattg
      181 ggaaacaatg aaagaagcat gtcaaatatt ggatgaattc ggtgtgtctt atgaaaaaaa
      241 ggtcgtttct gctcatcgaa ccccagattt gatgtttgaa tatgcacaaa acgctcgaaa
      301 aaaaggaatc aaaatcatta ttgcaggagc agggggagcc gcgcatctgc cgggtatggt
      361 cgcatcaaaa acaactttgc cggttatcgg tgtaccggtc caatcacgaa cattaaatgg
      421 tttggattcg cttctttcga ttgtccaaat gcctggagga gtacctgtag ctactacagc
      481 aatcggaaaa gcgggagcaa ttaatgctgg actacttgcg atacagatgc tttccatgta
      541 tgatttagaa ttggaggcta aattggctga aagacgttca atgttggcta aaactgttat
      601 agaaagtagt gatcaacttg gttaaacctt tgttgcctgg ttcaacgatc gggatcgtcg
      661 gtggcggtca attaggtcgt atgatggcga ttagtgcaaa agaaatgggc tttaaagtag
      721 gtgtgctgga tcctgtgagc gattgtccag ccgcacaagt tgcagattgg cacattgaaa
      781 gtgtctatga tgacaccttt gcgctgaaag agcttgccag aagaacagat gtcattacct
      841 atgaattcga aaacgtcagt gtagaggcgt taaatgcgat tttacccatg tcatttattc
      901 ctcaagggac agacttgctt gcgattacgc aagaccgcct gttagaaaaa tcttttttgg
      961 aaacgaataa catcgtgatt gctccttatg caacgatcgt cagtccgacg gatatccaag
     1021 atgcgattga cgggatcggc tatccttgtg ttttgaaaac cactcgaggc ggctatgatg
     1081 gcaaggggca gtatgttttg aaaagtcggg cggatctagc tcctgccatg gaccttttaa
     1141 gagaaggaac ctgtgtttta gaagcatgga tcccgtttga aaaagagatt tcgatcatgg
     1201 tggcaggaaa tggtcaagga gatttcacga cttttccggt cgtggaaaac atccaccaca
     1261 acaatattct tcatgaaacg attgctccag ccgcgattga tcaggatgtg atcgaggagg
     1321 ctgagcgtat tgcccgtgtg attgcagaag cagtgagcct ttcaggtgtt ttaggggtag
     1381 aaatgttttt aacaaaaaca ggcgggctat atgtgaatga attagctcct cgtcctcaca
     1441 attctggaca ttattctatt gaagcttgtt ctatgagtca gttcgatgcc catattcgag
     1501 gtatttgtgg ttggccatta ggagaaaaag tacgtttgat aagcaatgca gtaatggtca
     1561 atgttttggg agaacaactg ctggaaagct atcgcttgat tgagaataag ccagactggc
     1621 aattccattt ctatggaaaa aaacaagcaa aaaaaggtcg caagatggga catatcacta
     1681 tcttgacaga agacatccag caaacgctgg aagaaatcaa acaaacgaat atttgggatt
     1741 agaggagaaa aaacatgtta gaacgttata cacgccctga aatggggaat atctggacgg
     1801 aagagaacaa ataccgtgcg tggctcgaag tcgagatttt agcagatgaa gcttgggcag
     1861 aacttggtga aatcccgcaa gaagatgtga aaaaaattcg tgagcatgca tcatttgata
     1921 tcgatcggat cttggaaatc gaaaaattaa cacgccatga tgtcgttgcg tttacacgag
     1981 cggtttctga aacattggga gaagaaagaa aatgggtcca ttatggtcta acaagtacgg
     2041 atgtcgtaga tactgcttat ggctatctca tcaagcaagc aaatgatatt ttacgggaag
     2101 acttgaaacg ttttacggaa atcattggcg aaaaagcaaa agaacacaaa tatacagtga
     2161 tgatgggtcg gacacacggt gtccatgctg aaccaactac ttttggattg aaacttgcca
     2221 catggtattc agaaatgaaa cggaacatcg aacgatttga acatgcggca aaaggagtag
     2281 aagcaggaaa aatctcagga gcagtgggaa cgttcgcaaa tatcccgcca tttattgaag
     2341 agtatgtatg taaacaattg gggatccgtc cacaagaaat ctctactcaa gttctccctc
     2401 gggatctaca tgctgaatat ttctcagcga tggcgcttat tgctacaagt attgaacgtt
     2461 ttgcaacaga gatacgcgga ttgcaaaaat ccgaaacacg tgaagtagaa gaattttttg
     2521 cgaaaggtca aaaaggttct tctgctatgc cgcacaaaag gaatccgatc ggttcagaaa
     2581 atatggctgg tctagccaga gtggtgcgcg gacacatggt cactgcctat gaaaatgtca
     2641 cattatggca tgaaagagat atttctcatt cttcagcaga acgaatcatc ttgcctgata
     2701 ccacgatttt attgaattac atgttaaatc gttttggcaa tatcgttaaa aatctaacgg
     2761 tctttcctga aaacatgaag cggaatatgt ctgcaacatt tggtttgatt tatagccagc
     2821 gcgttatgct gaaattgatc gataagggaa tgacaagaga agaagcttat gatcttgttc
     2881 agccaaaaac tgcgtacgct tgggacaacc aagtgcagtt ccgcccgctg ttagaagaag
     2941 atccaaccat cacatcggta ttatctaaag aagaaataga cgatgccttt gattatcatc
     3001 atcatttgaa aaacgttgac gtgatattca aacgagtagg tttggaataa agaagagtga
     3061 gttgcaaaat tcaattaaaa tagaatagca aaaataacca aaaaagaagc agatccgtta
     3121 gcggttctgc ttcttttagt ttattatatt ttcttatcaa taataggaac ctgattttaa
     3181 aagcggctac ctctttaccg tgcctcttta catacaaaaa atagaggaat taagcgtaaa
     3241 tggcttggct attataacta aaatgctggt tatggagatg ttgcagcaag ttttcgagaa
     3301 aagcgtattc catctcttgt ccattgaata agatctggat acgataatga tttttgaaac
     3361 gcttctgttc atgaatctga ctgattgaaa gttcgccttt gtgttctgtc tgataaagaa
     3421 aagtcctttt acggcattca gcacagcgct tggttgctcg gcggattttt cggaatgtat
     3481 aaaaactagt atctttattg cataaataaa cgtctttgat cgacagcaaa tcattatata
     3541 gacaggaacg ttcttcagct gttaattctg ataaaaaaag ctgaccttgt tttattgcat
     3601 cttccatcgg caagtgcctc ctcgaaccca ttatccaaat ttttagttaa ttctaatata
     3661 ccatcattaa gtataaatgt aaacgcttgt tgatggtata agattttcgt ttctgataaa
     3721 acacgaacat taaaagaatc taatgcctta aatgtacata tttttgttga tttataaaaa
     3781 gatgattgct aaaatttaaa gtgaaaaaac gaacaaaaaa cgatgtattt gcttttaatg
     3841 taaataaaaa ggagagacgt acatggagaa gaaagaactt ttgtatgaag gaaaagccaa
     3901 aaaaatatat cttacttctg atgagaactt gatttggata gaatatttag atcaagcaac
     3961 tgcgttgaat ggcgcgagga aagaccagat aagtggaaaa ggagaattga ataatcaaat
     4021 cacaagtgca attttccgtt atctgactca aagaggaata aaaaatcatt ttattgaaca
     4081 attgtctaaa accgaacaat tagcaaaaaa agtacaaatt atccctctgg aagtggttat
     4141 ccgaaatatt gcagcaggaa gtttctcaaa acgtctaggt gttcccgaag gtaaagaatt
     4201 catcaaacca atcattgaat tctactataa ggatgaccag ttggatgatc catttatcaa
     4261 tgaagagcat atctatttct tacaacttgc aaaaccggaa gaaatcagtg agctgaaagc
     4321 ccaagcgttg gctattaatg aaattttgac cgaattattt gctaaggtcg gtttggtttt
     4381 agtagatttc aagctagaat ttggccggtc gaatggacaa attttgcttg cggatgaagt
     4441 aacaccggat acttgccgat tatgggatga acgtacgaat gaacatatgg acaaagacgt
     4501 ttaccgcaga gatctaggtg atttgatccc agtctatcaa gaagtatata accgtctatc
     4561 ggaactaact gaagaaaagg agcgagtatg atgtatttcg taaaagtata tgtaacctat
     4621 aaagattcag tgttagatcc tcaaggggag gcagtaaaaa aagcagtcca tcgtatggga
     4681 tatgaaacaa tcgaagatat ccgaatagga aaatactttg agatacaact tgctgatgca
     4741 gaagaaccaa tcgatacagt gattgaaacg atttgtgaca agctgcttgc aaatgtaaat
     4801 atggaaacat accgatatga aatcaaaaaa atggaggaag tataacatga aatttgcggt
     4861 catcgttttt ccaggttcaa attgtgacgc agatatgtta tgggcagttc gcgagatcat
     4921 gggcgctgat gccgaatttg tacggcatga tgagtcctct ttagaaggat ttgatggggt
     4981 tttactgccg ggaggattct cctatggtga ctatttgaga tgcggtgcga ttgctcggtt
     5041 ttctacaatc atggaagaag tgattcgttt tgctgaagaa gggaaaccag tattcggtac
     5101 atgcaatgga ttccagatat tgacagaagc aggacttttg ccaggtgtac tgagacgaaa
     5161 tgagtctttg cattttattt gcaaaactgt accattgaaa gtcaacccca agacggcctt
     5221 tacttcagag tatgaagccg acgaaataat ctatcttcca gttgctcatg gggaaggaaa
     5281 ttatttctgt gacgaagaaa cacttcaaga actaaaaaaa aataatcaga ttgcctttac
     5341 ttataatgac aacatcaacg gcagcgtgga aaacattgca gggattacga acaaagcagg
     5401 gaatgtgtta ggaatgatgc ctcatccaga acgtgcagta gaagacttgc taggatcaga
     5461 agatgggaaa cggttctttg cttccatttt aaaaaacttc ggaaaggtga ccgtatagat
     5521 gaaaacattg aaagaaccga cagctgaaga gatcaagaaa aaaagaatct atgccgaatg
     5581 gggattgact gatgaagaat atcgaatgat cgaagaagat atcttaggaa gaatgcctaa
     5641 ttatacggaa actggactgt tttctgttat gtggagcgaa cattgttcct ataaaaattc
     5701 taaaccggtt ctacgcaaat ttcctactag tggacctcat gtgttgcaag gccctggaga
     5761 aggcgcaggc atcgtcgaca tcggtgatgg actagcagtt gtcttcaaag cagaaagtca
     5821 caatcatcct tcagccgtgg agccttatga aggagctgcg acaggagttg gcggaatcat
     5881 cagagatatt ttcagcatgg gggctcgtcc aattgcgttg ctagattcat tgaggttcgg
     5941 cgaattagat aatccaagaa cgaaatattt actagaagaa gtagttgcag gtatcggcgg
     6001 atacggcaat tgtatcggca taccgactgt aggcggcgaa attgcatttg atccttgtta
     6061 tgaaggaaac ccattagtca atgcaatgtg cgtaggtttg attgaacaca aacatatcca
     6121 gaaagggcaa gccgctggcg tcggtaattc gatcatgtat gtaggtgcaa aaacaggtcg
     6181 cgacggtatc cacggagcaa cttttgcctc agaagaattt aatcaagaag aagaacagca
     6241 acgttctgct gtacaagtag gcgatccttt tatggaaaag ctgctgcttg aagcgtgttt
     6301 ggaattaatc ttgcatcatt cggattctct aataggtata caggatatgg gagcagcagg
     6361 acttgtttca tctagtgcgg aaatggcttc aaaagctggc tcaggactta tattgacatt
     6421 agatgaagtc ccacaaagag aaactgggat gacaccttat gaaatgatgt tatcggaatc
     6481 acaagaacgg atgttgattt gtgttaaatc aggagaggaa gaaaaaatcc aagaactatt
     6541 tcagaagtat gatctagatg cagtcactat cggtaaagta acagacgacg ggcaatatcg
     6601 gctatatcat caaggagaag aagtagcaaa tctgccagtc gatgcattag ctgaagatgc
     6661 accagtttat cataaagaaa tgaaagaacc agctcgaatc gtagaatttc agcagttagc
     6721 tccttatcag ccagttatcg aagaacctgg acaagtattg cttgatctgc tgcagcaacc
     6781 gacgattgct tcaaaacgtt caatctacga aacttatgat tcacaagtac aaaccaatac
     6841 agttgtccgt ccaggaagcg atgctgctgt gatgagggtt agagggacga ataaagcgtt
     6901 agctatgacc acagactgca acgcgcgcta tctgtatttg gatccaaaaa tcggaggaca
     6961 aatagcagtg gcagaagctg ctagaaatat tattgctagc ggtggtaagc ccttggcgat
     7021 caccgactgc ctgaactatg gttctcctga taaaccagaa gttttttggg aattatcgac
     7081 ttcagctgac ggaattgctg ctgcgtgtga aacgttagga actcctgtta tttcaggaaa
     7141 tgtctcttta tataatgaaa cggatggtca agcaatctat ccaacaccaa tgattggtat
     7201 ggtcggattg attgaggatc ataaacatat cactactcag gaattcaaaa aatcagggga
     7261 tctaatctat atattaggta agacgtttgc agattttgat ggcagtgaat tacaaaagat
     7321 gcagcttgga agaatcgaag gtgtgatccg aaactttgat ctatccattg aaaagagaaa
     7381 ccaagagtta gtattgacag ccatccaaaa cggattgatt gaaagtgctc atgactgttc
     7441 agaaggtggc ttggcaattg cactggcaga atcagctttc aaacatcagc tagggatatc
     7501 tgtacagttt gaattgtcct ctgctcaatt atttgcagaa actcaatcaa gatttgtgtt
     7561 gacagtcgca ccggaaaata agacacgatt cgaagaaatg atgggagatg ctgctgtttt
     7621 agcaggtaag gtgacggatg aagcgataat cgaaatttca gcaactgacg gtcagatcaa
     7681 aatagaaact gcagtagcaa gaaagtgctg ggaggatgca atcgtatgtc ttatgaagtg
     7741 aagagtttaa atgaagaatg cgggatcttc ggtgtttggg gtcatccaga cgccgctagt
     7801 gtggcatact tcgggctcca tagtcttcag catcgaggac aagaaggtgc aggtatcgtg
     7861 tccaatgatc gaggaaagct taaggggcat cgagatttgg ggctgctttc tgaggtattc
     7921 aaagatcaaa gaaaattgca gcagttaaca ggagaagctg caatcggaca tgtacgttat
     7981 gcaacagcag gcaatggaag tgtggacaat attcagcctt ttctattcaa atttcatgat
     8041 cagtcgatcg gcttagcgca taatggtaac ttgatcaatg ctcgttccct tagaaaagag
     8101 ttagaggaaa atggggcgat cttccattct aactccgata cggagatatt gatgcatttg
     8161 atccgacgaa gtgaagaaac agatttttta aataaattaa aaagtgcttt gaatgaagtc
     8221 aaaggtggct ttgcctattt attaatgaca gaaacggcaa tgattgccgc tttggaccca
     8281 aatggttttc gtccattagc aatcggacaa atgaaaaacg gagcatacgt gattgcctcg
     8341 gaaacttgtg cattagaagt tattggggca cgttttataa gagatgtcca gcctggtgag
     8401 atagtgatca tcaatgacga aggaatccag attgattgct ttacgaagga cacgcagctt
     8461 tctatttgtt cgatggaata tatctatttt gccagaccag attcaaacat cgcagggatc
     8521 aatgtgcata ctgcacggaa aaatatggga cggaatttag cgaaagaatc gccagtaaaa
     8581 gcagatatgg tcgttggcgt acctaattct tctttgtcag ccgcaagcgg ttatgcggaa
     8641 gctagtggaa tcccttatga aatcggatta gtaaaaaatc aatacattgc ccgaacattt
     8701 atccagccaa cgcctgagct aagagagcaa ggtgttcgaa tgaaattatc tgctgtacga
     8761 ggagtagtgg aaggtaaaaa ggtgattatg gtcgatgatt caattgttcg gggtacgaca
     8821 agcagacgga tcgtccatct tttgaaagaa gcaggggcaa aagaagtcca tgtgaggatt
     8881 gcttctcctc ctttgaaata tccatgtttt tacgggattg atattcaaac gcgtaaagaa
     8941 ctcattgcgg caaatcacac gattgatgaa atcaaagaat gtatcggtgc agattcttta
     9001 ggttttttaa gtgaagaggg tctgatcgaa tcgatcggtc tggaaaaaga tgctccgtat
     9061 tcaggattgt gtatggctta tttcaacgga gattatccga cgccgttata tgactatgaa
     9121 gaaaaatatc aggaatcact aaaagaaaaa gtatcgttct tttaaaagca gaccaagaag
     9181 ggagtatatg aattcaatgg caaatgcata cgcaaaagct ggcgtagatg tcgaagctgg
     9241 atatgaagta gtagaacgga tcaagaagca tgtcaaaaaa acggaacgtt taggtatgat
     9301 gggcgctata ggaggattcg gcggttgttt tgatttatcg gtctttcaat taaaagagcc
     9361 agtccttgtc tcggggaccg atggcgtggg gacgaaatta gcagtggcaa tcgaagcaga
     9421 caaacacgat acaatcggga tcgactgcgt agcgatgtgt gtcaatgata tcttagcaca
     9481 aggcgcagag ccgttatatt tcttagacta cttagcaacg gggaaaaatg atccgttacg
     9541 tttggaaaat gtagtaaaag gtgtagcaga tggatgtgtt caaggaaata tggcgctgat
     9601 tggcggagaa acagctgaaa tgcctggtat gtactcaggt aatgattatg atctagccgg
     9661 atttgctgta gggatagctg aaaaatctca attattgaca ggagaaacga tcaacccagg
     9721 agatgtgttg cttggaatag catctagcgg gattcattca aatgggtact ctcttgttcg
     9781 caaaattttc tttgagcagc agcagttttc tttatcatca aaactgccag aattgaaaaa
     9841 aacacttggc gaagaattgc ttgagccaac gaaaatctat gtttcttcct tgcttccgct
     9901 gatcaaagaa aagaaaataa aaggagctgc acatatcact ggtggagggt ttgtagaaaa
     9961 tatcccgcga atgttaccag aatctatggc tgcacaaatc aaacttggct catggccgat
    10021 tcttccaata ttcggctgtt tacaaaaata tggtgaaatt cctgagatgg agatgtatga
    10081 aatcttcaat atgggcatcg gtatggtcct ggcagtcgaa gaatcgaaag taaaagaaat
    10141 agtacacgaa attgagcaag ctggcgaaaa agtttatgaa atcgggaaag tgatcgccaa
    10201 agaaaaacat cgtgtggtgt ttataggaga agaacgatga gaatcgctgt ttttgcttct
    10261 ggaaacggaa gcaactttca agcgctagcc gattatctat cgaaaaaagg attggaatct
    10321 tcgattgact ggttgttttg tgatcagcct gaagcatatg ttttgaaacg tgcaactgcg
    10381 ttgagtgtac cggcagattg tttttcacct aaagaatttg attcaaaaaa agagtatgaa
    10441 gaagcaattt tgcataaatt gaaagaaaaa aagatcgatt tgatcgtatt agctggatat
    10501 atgcgaatca ttggaccggt attgctgaaa aattatgata aaagaatcat caatatccat
    10561 ccgtctcttt tacctgcctt ccctggtttg catggcatac gtgatgcctt tgaagctgga
    10621 gtaaaagaaa cgggtgtcac aattcattat atcgaccaag gtgtagatac tggaccaatc
    10681 atccggcagg aaaaagtgag aattgaacag gaggacacgt tcgattcttt agaagaaaaa
    10741 atccatagag tagagcatcg aatctatcca gaagtgatta gtgaaattat tgaaaatgga
    10801 ggctatctga aatgacaaga gcattgatca gtgtatcaga taaaactggg attgtagcat
    10861 tcgcccaagg tttgattgaa caaggagtag aaatcatttc aacaggagga acaaagcatg
    10921 cgttggataa agctgggatc aaaacgcttt cgatcgaaga agtgacaggg tttcctgaaa
    10981 tgatggatgg ccgtgtcaaa acacttcatc ctaaaatcca tggtggtctt ttaggaagac
    11041 gcgatctccc tagccatatg gaggcaatgg aaacggaaaa tatccaacca atcgactttg
    11101 tttgtgtcaa tttatatcct ttcaaagaaa cgattatgaa accagatgtc gaaatagcag
    11161 aagctattga aaacattgat atcggtggtc ctagtatgct ccgtagtgca gcaaaaaacc
    11221 atgaatttgt cacctctgtt gttgatccat ctgactatga agaagtactt tcagaattac
    11281 gtcagtatgg gcatacttca ctttctttca gacaaaaact agcggcaaaa gtctttcgtc
    11341 atactgcggc atatgatgca ctgatcgctc aatacttaac ggattgggtg gaagaaaaag
    11401 aaccggaaaa attgacactt acgtatgaac gaaaacaaac gcttcgttac ggtgaaaata
    11461 gccatcaagc cgctgccttt tatcagtccg ctcttctgga aaaatattcg atcgcagcag
    11521 caaaacaact ccatgggaaa gaactttctt ataataatat ccgagatgca gatgctgcgc
    11581 tacggatcat gaaggaatat gacgagccaa cggtagtcgc cttgaaacac atgaatcctt
    11641 gtggtattgg gacagcatct acgattttag aagcgtggga agcagcgtat gaagcagatc
    11701 ctgtttcaat ctttggtggg atcatcgttt tgaatcgaga agtagatctg ccaacagcag
    11761 aacagatgca taagctgttt ctagaaatca tcattgcccc ttcttataca caagaagctt
    11821 tggaattact gcaaaaaaag aaaaacatcc gattactgac agtagacttt gcgcaggaag
    11881 aaaatcaaaa tcgagaagaa acggtctcag tcttaggtgg acttcttgta caagatcaag
    11941 atctggcaat agaaaatgaa gaagagtgga aagtcgtgac aaaacgtaag ccgactccag
    12001 cagaagcaaa agcattagcg tttgcttgga gagctgtcaa acatgtgaaa tctaatgcga
    12061 tcgtactggc aaacgaacgt cagacagtag gaatcggagc cgggcagatg aatcgagtcg
    12121 gttcagtaaa aattgcgatt gaacaagcgc aagagaaaat ggaaggtgca gttttagcta
    12181 gcgatgctta ttttccaatg gatgattcgg ttgaatatgc agccaaacat ggtataaaag
    12241 caatcattca gccaggagga agcatcaaag accaagattc tattgatatg gcgaataaat
    12301 acgatctagc aatggtcttt actgatgttc ggcattttag acattagaga ggagtcggat
    12361 aaatgaacat tttggtgatc ggtgcaggtg gtcgtgaaca tgcaataagt aaaaaacttt
    12421 tagccagtcc gaaggtggat acggtgtatt gtgcgccagg aaatccgggg atgacaaaag
    12481 atggtatcaa ttgtgtcgct atcagtgagc gagatcattc agcgttaatc cattttgtta
    12541 agaaagaggc tattgattgg agtatcgttg gtcctgaagt tcctttgtta aatgggatcg
    12601 tcgatgattt tatggcggaa ggattgaaaa tcttcggtcc gacaaaagca gcagctctga
    12661 tcgaaggttc gaaaagtttt gctaaacaat taatggatac ttatcaaatc ccgacagcta
    12721 aatccagaag ttttgaatgt tacgaaaaag cagcagcgta tatcaaagaa aatggtgcgc
    12781 caatcgtagt gaaagcggac ggcttagcag ctgggaaagg tgtagtcgtg gctaccacag
    12841 tgaaggaagc gctggatgcg gcagaacaaa tgttgaacca gcatcgattt ggggatagta
    12901 gcaaaaaaat cgtcgtagaa gagtttttgg caggagaaga attctccctt ttggcgtttg
    12961 tccgtaatac caaggtgtat ccgatggtca tttcgcaaga tcataagcgg gcatttgata
    13021 acgataaagg gccaaataca ggcggaatgg gtgcttattc accagtaccg caaatttcaa
    13081 aaaagatgat tgaaaaagct gtggaagaag ttttaaaacc agcggctttg ggaatgaaag
    13141 aactaggacg ctctttcacg ggtgttttat atgcgggttt gattgctacg aaggaaggac
    13201 caaaagtgat cgaattcaat gcgcgtttcg gtgatcctga aacgcaagtc gttctttctc
    13261 ggttgaagag tgactttgcg caagtaatcg atgatttatt agagaatcgt acagtagagt
    13321 taaagtggca gcaagaagga tacaatgtag gcgttgtggt tgcagctgcc ggttaccccg
    13381 aagaatatga gacaggaatg gttctaccag attttctgga agaagagctt tctgtttact
    13441 atgctggagt ttcctcaaat aacggcaaac tagttggagc aggaggacgt ctttatttag
    13501 tagaagcata cggtgagacg atcaaagaag cacagaaaaa aatttacacc gcgttaatgg
    13561 ataaaacgac agatggaaca ttttatcgta cagatattgg agaaaaagct gccaatagcg
    13621 aactgatcgt gaagtaaatc agctgcttcc atttataaaa aatacaacaa acataatcgg
    13681 aagcgccgat tttagtgaag ccttttcata aagagtgtgg tacacttttg ttgtgtatat
    13741 aaataaggag gagttcagat gaaaaaaatt caattaggta cttctgatca gaaagcagct
    13801 gcagtgatct taggctgtat gcgtatcaac aatgcggaag atccagtaaa agtgattgag
    13861 acagcttatg aaaacggcat tgattttttt gaccatgcag atatttatgg aaacggtgaa
    13921 tgtgaagaaa tctttgcaga tgctctagca aaaacatcaa tcaaacgaga agatcttttt
    13981 attcaaacaa aatgtggaat cgttccaggt gtgatgtttg atttttctaa aaaacatatc
    14041 attgaatctg tagaaaaaag tttgacccgt ctgaaaatgg attatgtgga tgctttactt
    14101 cttcatcgtc cagatacatt ggtagaacca gaggaagtag ctgaagcgtt tgatgaattg
    14161 gaacggtcag gaaaagttcg ttattttggc gtcagcaatc aaaaaccaat gcaaatcgaa
    14221 ttgctgaaaa aatatgttcg tcaaccatta gtagctaatc agctgcaatt tggcttgaaa
    14281 catactggaa tgatcgatca agggattcat gtcaatatga cagatgaagc aagtatcgat
    14341 catgacggta gtgtacttga ttattcccga ttgcatgata tgacgatcca agcatggtca
    14401 ccataccagt atggattttt tgaaggtgta tttatcggga acgaaaaatt ccctgaatta
    14461 aaccaaacat tagaagaact agcagctaaa tatcaaacga caccaactgg cttggcttct
    14521 gcttggattt tacgccatcc agccaatatg caagtgatcg ccggtacgat gaacgtaggg
    14581 cgaatcaagg aaatcgctca agcttcagaa attaatatgt ctcgggaaga ttggtacgaa
    14641 ttgtaccgtg ctgctggaaa cattttacct taaagattgt cataagcagg tcaaaagggc
    14701 tataaattaa aaagacagaa taacttgaaa gctgttttca gcttcgagat attctgtctt
    14761 tttttattgc aaatggaaag agtatcattg tacggatttc gtttattagt tatttttgtt
    14821 tttaaggaaa agctgtgtcc gatattcttt tggagaaatc ccttgttttt tcttaaataa
    14881 ataactgaaa tagttagggt cattataacc aatttcaaaa gcaatatctg aaatacgata
    14941 gtctgattgc tgcaataatt tttttgctaa ttcgattctt tgttctgtca gataatcgat
    15001 gaatgtttgg ttaaacgatt gagaaaaaat cgtactgaaa tgagagggac ttaatgcaac
    15061 ttgttgagcg acccaagtta aggacatatc tggatcggta aaattttctt tgataaaatc
    15121 caatgcatgg ttcaaaattg gctgatattt gttccttgaa ggctgtgagg gcagagcagt
    15181 taagaaagaa atgaacatat aaactgtcga acggtaaagc gagtcgtcgg cgctgattgc
    15241 taagcgttga ctggtggatg tgcaatgaac catttcttgc gggattttct tttctttcag
    15301 gcagacatgc tggagtagct tagtcaagac aaataaacga tataatcgtg ttcgagttgg
    15361 tgtgccttgt ggtttcgtca agtgttcgat gtacttagat acttcgtttt tcgataatga
    15421 agacaaatca agtgagaggt caaagtaaaa gacagaatca actggaagtt tcgcttcttg
    15481 aatcaaatcc tcatagctga taatttgttc tttaagggaa ctggcggttt caagtatcat
    15541 ctgcgtgatc tcaaaagatg caggtaattc gctgattctt tctgcgacag gaccaatggc
    15601 gacagaaaaa ttattttttt gtcccttttc taattcatgg accaacgttt gggctacctg
    15661 ataactggtc tcgatcacat tttcttcaag agaatcaact attaaaaatt tgatgaatcg
    15721 agaggaaaca gcagagaaga tcacttgttg attctcccta aataaaaact tcaaataatc
    15781 actgagatga gcgtaatctt caaagtctgt actgacttgg tttgctgcta ataatacggt
    15841 catttttttc cctgctaaag agcgattgaa agattcagca agtttcaatg tttcactgat
    15901 cgtaagtgag cctttgaaca aaccgttcaa gaaatgattt tttttcaatt cgaaaacaag
    15961 atcagaagaa gtgtgcgttt ctttttgctg caaatctaaa gcagttactg ctttttctat
    16021 tgcttgtttc aattcgctac ttttaatcgg tttaagcaga taatcatctg cctgtacctg
    16081 gattgctcct ttcatataat cgaaatcgtc gaatccacta ataaatatga gacgagtcca
    16141 aggaagtagt ttccttgctt cttttgaaaa agtcaacccg tccatgaaag gcatttggat
    16201 atcagtcaga atgatgtcgg gctgtaagtc gataattgaa gacagtgcca tctcaccatc
    16261 tccagcttcg ccgacataag tgattggata ataagaacca aggacagtta tttgttcccg
    16321 caagttattg cgaatgaggt attcgtcttc gatgataaaa actttataca tgtttgtttc
    16381 ctccaattgt tttgggagat agagggatca tcagtgtgac agttgttcct tctcggtatt
    16441 tgctgtcaat cgtgataggt ttttgatcat ttccgtaata aagaaggaga cgcttgttta
    16501 cgttgtaaag tccgtagccg atactgaaat ctgaatcgat ccctttggct aactctttac
    16561 gtatttctgc cagtttttct gcagggatgc caatcccgtt gtctataact ttgaattgga
    16621 tcatctcatc aattttgcat gcggtaatac gaataaatcc tttgtgacgg acgtacttcg
    16681 ttccatggta tacagcattt tcaatcaatg gctgcaagat catttttaaa atagaaacag
    16741 gaaaaagtgt ttctggtata tcgatcttat agtcaagcat atggccgtat cttattttta
    16801 agatgactag ataatcattg atatggctaa tttctttttc aagaggaatc cagtcttgtc
    16861 ctttgcttaa agacgtacga aaaaagccgg aaagtgcatg ggtcatagtc agagcttctt
    16921 caaagcagtc ttgttcgatc aaagaaataa tggcatccaa actgttataa ataaaatggg
    16981 gagtaatctg agcttgcaat acacgaactt cactttgtgc taaatggtat tgtttcaaag
    17041 cgttctcccg taaagactgg tttagatctt ccgccatttt attcatgcta gcggtaagtg
    17101 tttgtaattc cagtgtttcc ggcagggtta aacgaaagcc aaggttccct gaagaaagtt
    17161 gttctgacat ctcgatcagc tgattgatcg gttcttggac ctttctctct aaaaagcgac
    17221 gattcttttt cgcatagaaa aaaatggtac taagaactcc aagttccact attgttaata
    17281 gaatgagcga gcggacaagt gtcgtatttt ttttgcttgc taagtcgatc tccacacgaa
    17341 caaaatcttg taagatatct gacagcagtt gagtgacaga atctacctga ttgatgatct
    17401 cttcattggt atccataaaa tcttttgcca agatattatt gatcagctcg ttttgataat
    17461 cttccagtgt atcgatcgta cgtagcgata catctaacgt actaatttct ttaagtgtat
    17521 ttgtattatt tttgatctct cgaatatcat tcctaagttc ttctaggata ttttcctcat
    17581 tgaactcatc tacggttact tggccagaaa tcaagtccca catactttcc aaaacttttt
    17641 gatcaacctt caaagcaatt gcatctgctt tttggatatt tgcgatcgtc tgttgataaa
    17701 tcaaaagctg ttgcgaatat aacagtatgc tcagtagcaa agggataaca gcaaatagaa
    17761 gcatggcttt gtttgcttgg gcaaaaatct gttttatgct taaatttttt gtcatgatac
    17821 ttctccagtc aataatttct ggttgggatg ttagctatat tctgctggga aaagacagtc
    17881 tcggaaataa aaatatcttt aggtaatgtt tttcctgttt gaatatttcc aaaataacga
    17941 gtaacggtat tggctacata ccagccgaca tcaggtttac actcgactac acaattgacg
    18001 attcctgagc gtagcttctc gatcatatct tcttgtccat caatagttac aatgatcaaa
    18061 tcttctccag gtttgattga tgtttctttt atcgcagcta aagcaccgtg agtcatttca
    18121 tcactatggc tgtataatac atcgatttcg gataaatcat ttgtttctaa atatcgtttc
    18181 atctggtctt ttcctttcat ccgaataaaa tcaccgacaa ttcggtgaac aatagtcatc
    18241 ggaggtttat ggtcgatggt tccatctttt tttagggttg gtttacgatt gattgattcc
    18301 aaaaatcctt cggagcgtag cttggtcggt gtggagttct ctgaaccagc aagttctagg
    18361 attctgattt cctgtttctt tgaatttgtg aagtgattgc tgacaaataa acccgcccga
    18421 tttccttctg ctttgaaact ggggccaatg tgggtgacga aaagctcggg atcattcgtt
    18481 tgaatatgtc tgtcgacaag tatgacaggg atccctgctc gctttgcttc ttttaataca
    18541 gagtcccacc cggattcttg caaaggagta aaaatgatca tatccacttt ataggcaatg
    18601 aacgtccgta tatcttggat ttgccgttcc tgattcatgt agccatttcg gtagagaact
    18661 tggtaatctt ctttttcaag cgcagtgata attgattcag tatgcttttt ccgccagcta
    18721 ctctccgttc ctgattgcga aaaaccaacg actagtgggt caggtgcttc ctttgtatta
    18781 ggtgtacacc ccatcatttg gtaagcgatt acaacaaggg ctaaaaaaaa taaaacacgt
    18841 ttcactttaa atcactccat ctggatttgt tagtactatc ttatcagata aaagggaaaa
    18901 tcaaagtcaa aaggaatcaa tcgaaaaaaa tccaagaaac ttaaaaaaat ccaagacaaa
    18961 aaaagtacac ctaaaaaatt taaagtaaat cccataaaaa aatcaatgtt aacgctttct
    19021 ttattagcta tactcggtgt atcaattgaa atgggggata aaaaatgaaa aacaaattta
    19081 gaaagattgt tgggactatc gctattttga ccgcaagcat tctattaagt gcgtgtggca
    19141 attcaggatc tgcagatgat agtacggggt atgtaggaat cgctatgccg accaaatcag
    19201 cagaaagatg gatcgctgat gggaacaaca tggtctcgga attagaaaaa ctgggttata
    19261 aaacagatct tcaatatgga gaagataaag tagaaaatca agtagcacaa atagaaaata
    19321 tgattacaaa aggggtagat acacttgtga ttgcttctat cgatggttcg gctttaactg
    19381 atgtgttagc taaagcaaaa gaagcagata tcaaggtgat cgcctatgat cgtttactga
    19441 tgaattcaga aaatgtcgat tattacgcga cttttgataa ttttggagta ggtgtatcgc
    19501 aagcagctta tattgaagag cacttaggat tgaaagaagg aaaaggacca ttcacgattg
    19561 aattatttgg tggctcgcct gacgacaaca acgcactaat caattacaac ggagtgatgt
    19621 ctgttttaca accatatatg gacaacggtc agttggtcgt gccatctgga cagacgagtt
    19681 tcagtcaaat cgctacgctt cgctgggatg gttctacggc gcaagcaagg atggataatc
    19741 tgttaagcgc aaattatact gaccaaacat tggatgctgt tttgtcgcca tatgatccaa
    19801 taagtttagg aatcatttcg tcattaaaag gcgtggggta tgggagtgaa agtaaacctt
    19861 tacctgtgat tactggacaa gatgcgacag ttgctggcgt taaatcgatc attgccgggg
    19921 aacaaacaca aacgatcttt aaagacacac gtatcttagc aaaaaatacg attgaaatga
    19981 tcaaagcaat cagtgatgga gaagaggttc cggtcaatga tactgaaact tatgataatg
    20041 gggtgaaaac tgtccctacg tatttagcaa atacagtatc tgtcgataaa gataattatc
    20101 aagcagagct gatcgacacc gattattata aagaatccga tctgaaaaat taagaggaac
    20161 caaacaagga actaaagaga tctaagtcag agagaggata agaattatgg cggattgtat
    20221 tttaaaaatg gcagatatca ccaaaaaatt ttctggaatc cgagcgttag attgcgtgaa
    20281 tctgatgatt gaacgtgggg aaatccatgc attgtgcgga gaaaacggag cgggaaaatc
    20341 aacattgatg aatgtcttga gcggcttgta tccatatggc tcctatgacg gggaaatctt
    20401 ttatgatgga aagctgtgtc aatttaaaga tttgaaagat agtgaagaaa aaggcatcgt
    20461 catcattcat caagaattag cactaagtcc ttatctttca ataaaagaga atatttttct
    20521 cggaaatgaa caagcagaaa gaggcgtgat tgattgggaa ctgacagaaa agaaaacata
    20581 tgaactgctg aataaagtcg gtttgtctat cgacccgaat cttctagtat cccagattgg
    20641 cgtagggcaa cagcaactag ttgaaatagc taaagcatta tcaaaatccg tccgtctgtt
    20701 gattttggat gagcctaccg ctgcattgaa tgaagaagaa agcgcgaatt tgctgagatt
    20761 gatccgagag ttgaaagagc aaggtgtaac ctccatcatt atttcacata agctgaatga
    20821 aatcgtggat atcgcagatc gaatcaccat tttacgggat ggacagacga ttgagacact
    20881 agaaaatgaa atgatcagcg aagagcatat tattcgcggg atggtgggca gagatttagt
    20941 tgatcgttat cctgaaagaa aaccggatat tggagaagtt tattttaaag tagaagactg
    21001 gacggtctat caccctcttg atcacgagcg aatcataaac gaccacatca acttgcattt
    21061 gaaaaaagga gagattgtag gaatagctgg actgatggga gcgggcagaa ctgaattcgc
    21121 tatgagtcta tttggacatt cttatggaag caagatatcc ggacgtatat taaaggaagg
    21181 ggcagagata tcagttaaga ccgttccttc agccattgca aacggactcg cttatgtctc
    21241 ggaggaccga aaatctttag gactgaatct gctgatggat attcgggaaa acgtgacgct
    21301 gagcagtttg gataaaataa gtcaaaaagg agtattagat aaagaaaaag aagtaactga
    21361 ggcagaaaat ttccggaaaa agatgcgtat aaaaacaaac tccatccatc aaattgtcag
    21421 tagtcttagt ggtgggaatc aacaaaaagt cgttttatcc aagtggctga tgacacaacc
    21481 cgatattctt tttttggatg agccaacccg tgggatagat gttggggcaa aatatgagat
    21541 ctacacgatt atcgaagaaa tggcagcaaa tggcaaagct gtctgtctta tctcttctga
    21601 acttcctgaa atattaggga tgtgtgatcg aatctatacg atgaacgaag gacgtattac
    21661 aggagaagtc tcgcgagaag atgcagatca ggaagtcttg atgaaattaa tgacgaaaga
    21721 agaggtgggt taagttggaa caggcaaaaa atcttacgga taaaaaagag aaaaaactaa
    21781 atttgaaaag tagagcagct gatctttttg gtcgctacag catggtgatc attttagcgg
    21841 gactacttat tgcgtttcaa ttgatgacgg atggtatttt ctggcgtcca ttgaatataa
    21901 ctaaccttgt tctgcaaaat agccatatat tagtactagc tgcagggatg ttattagttg
    21961 tgctcttagg tcatgtggat ttgtcggtag ggtcagtgat ggcgtttgtt ggtgcaattg
    22021 cgggcatgat gatggtcaat cgccaaatga acccgtggat gacagttatc gcatgtcttg
    22081 cagtaggcgg gctgatcggc gcatggcaag gtttttgggt agcttatgca gggataccgg
    22141 cgtttatcgt gactttagca ggattgctga tgtttcgtgg actgacacaa gttgttctag
    22201 gaggccaatc tctagcacct tttccagctg ttttccaaaa aatgtctacg ggttatttac
    22261 cagacttaag cgaaggaagc agccttcact tcttgacatt gattttagga atgatcatca
    22321 cgatcgtgtt agttgttggt caatggcgaa caagaaatcg tcggaaaaat catttatttg
    22381 aagtggaatc tccaacagct ttcttggtaa aggcggtatt tacagcagtc gttgtactag
    22441 gaataactta tatctttgcc tcttatcaag ggtatccgat tattttgatc attcttggtt
    22501 tgatcgttgc agcatatggg tttttgacaa ataaaacagt agcaggtcga cagatttatg
    22561 cgacgggcgg aaatcgaaaa gcggctgaac tatcagggat caaaacaaag cgaatcacgt
    22621 tctgggtatt cgtcaacatg ggagtcatgg cagctttagc tggattagtt ttagctgctc
    22681 gattgaatgc ggcaacacct caagcaggta catcgatgga actggatgca atggcggcag
    22741 tatattttgg tggtgcttcg acttccggcg gcattggaac aatcatgggt gcgattgtcg
    22801 gtggcttggt catgggtgta ttgaacaatg gcatgtcgat tttaggcgtc ggggtagact
    22861 ggcagcaggc aatcaaagga ttgatcttgc ttttagccgt tgtgctagac ttgtataata
    22921 agaaaaaacg aacaacgtag gagaacgccg gttattcaga aaaaagactg tgacaagaca
    22981 tttgtcacag tctttttttc gctaaaagcg aacattcatt gtttcgtttg tatagactaa
    23041 aaataatata tgttttttta ccattcgtat aaattctctt tttgctcttg atttttttgc
    23101 ttaattgagt taaattacat agggcgctaa aaaaagcctt gattaaatta ataatgttcg
    23161 tgttttgctg gatgtgaaca tcaggaggat aatgtaatga aagtatttga ttacgaagat
    23221 attcaattaa ttcctaataa atgtatcgta aatagccgtt ctgaatgtga tacaacggtc
    23281 acattaggta aacatacatt caaaatgcca gtagtgcctg ccaacatgca gacgattatt
    23341 gatgaaacaa tcgcagaatt cttagcggaa aatggttatt tctatatcat gcatcggttt
    23401 gatgaagcag ctcgaattcc gtttatcaaa aaaatgaaaa agcgtggatt gattacctcg
    23461 atcagtgttg gcgtcaagaa agaggaatat tcctttatag agaagttggc agaagaatcg
    23521 ctaaatcctg attatatcac tatcgacatc gcgcatggac atgctaactc agttattgat
    23581 atgattcagc atattaaaaa atatttgcct gaaacttttg tgattgccgg taatgttggt
    23641 acaccggaag ccgtccgtga attggaaaat gcgggcgcag atgcaacaaa agtcgggatt
    23701 ggtccaggaa aagtatgtat tacaaaaatc aagacaggct ttgggactgg aggctggcaa
    23761 ctggcagctc ttagatggtg tgcgaaagca gctcgcaagc cgatcatcgc tgacggtggg
    23821 atcagaacac atggcgatat tgcaaaatct gtccgttttg gtgcgacaat ggtcatgatt
    23881 ggttcattgt ttgccggtca cgaggaatct cctggcgaaa caaaagtcga aaatggaatc
    23941 gtgtataaag aatactttgg ttctgcttca gaattccaaa aaggcgaaaa acgaaatgtt
    24001 gaaggaaaga aaatctggat ccaacacaaa ggatctttga aagatacact agtggaaatg
    24061 caacaagacc ttcaatcatc tatttcttat gctggaggaa gagatctgga agccattcga
    24121 aaagttgact atgtcatcgt gaaaaattcg atttttaatg gagacacaat ctaaaaaata
    24181 aataaaatat taggatgttg tgaaaaagtt gtcctgcatt aagtaataag aacagccaaa
    24241 caaaaatagt tctttatatg ttttgtttgg ctggaattat tatcgcagat acaagctttt
    24301 gaacatcatt attcagaata agggactgtg ataagatttt tgtcacagtc ccttatgttt
    24361 tgcaaaaaaa gtttttcttt tttctcgtaa attcggtaaa atcaaaagaa aaaatagaca
    24421 atagaaaaga gaagcttatg tgggaaaaag tagagaaaaa acaaggaccg atttatcgtc
    24481 aaattatgga acagattatg agagaaatcg aaaacggaag gatcaatcct ggaagtcaac
    24541 ttgaatcaga acgaaaacta gggcaacttt ttggtgtgaa tcgttccacc gttgtccatg
    24601 cattggaaga gcttagggag cttggggtac taacgagcaa acgtggcagc ggatggtttg
    24661 ttaatcagac tgaatggggg aaattcgctg ttccaagaat cgattggcgg caaatgatct
    24721 ctcctcgtta tgagcagacg gactggtatg agcaaaaaat aagagaagca aagcagatcc
    24781 aaaaaacatc gtttcttgat ttatacgcta gcgagatgcc agatgagctt ttgccgaatt
    24841 ttgagtttcc ttcttattct cttgaagaaa tcttagcgga agaaaaagaa atggatcttt
    24901 taggctatac tccgttacgt aacaaaatca aacggtattt ggaaagtaaa tttgaattgg
    24961 cattttcccc caaccagctt cttgtgactg gtggcggtca gcaagcaatc tttttgattt
    25021 tacaaacgat tctttcagct ggagagggga tagctgtaga gtcaccttct tttttttacc
    25081 gattacctct tttcaaagct tcaggaacgc gtttatttgg gataccgatg gatgaagaag
    25141 ggatcgactt agaaaaactg aaagaatcga tccataaaaa taaattgaaa gcagtgctta
    25201 tcaacccgaa ttttcaaaac cctacaggaa aagtgatgag tcaaaaacga cgagaaaagc
    25261 tagtagaatt gtgtcgacag tatcggcttc caattattga agacgatgta tttacggatc
    25321 tttcatttct aaaccaagaa gaagtccttc ctatacgaac gattgatccg gataatgtgt
    25381 tatacgtcgg ttcgttatct agggttttag gaaaaacaac gaaaatcggt tgggtgatag
    25441 gacctgagac ttttatctcg caactagctc aagcgcaaga aatgatggag ttttcgatga
    25501 atatccttac acaaatcaca gtggctaatg tatttaatga agcaattgat ttgaaaatgc
    25561 aagatgtaag aaaaaagttg aaagaaaaaa gccgactgat aagcgcttgg ggagattcac
    25621 aatcgttttt caagcttcat tcaattttag gtggttacta tgcctggatt acttgggatg
    25681 ggaaaaaact agaccccgaa ctagcagaaa aagtagtaga tatgggtctt ggaattgcac
    25741 caagtctact ttttgggcaa gatataaatg gaatcagaat caattacagt aggctggatg
    25801 aaaaccaaat accgttgttt tattcaaaga tgaaaattct agaaaagtgg ttaacagaat
    25861 aatttcacaa agtaaataga ttaaagaata taacagcttg gatttaaatt tcaagctgtt
    25921 tctttttttt cggaaaaaga atacgatata aagaaactaa gaaaaaacca ttatttgtac
    25981 gtccgttcac gatgtgtaaa taaaataaat gtttgcaaca aaactgtata acaccaacgt
    26041 ttttaaatag aaacagatgg ttgtaagttt gattagaaaa tagaaaaaat gcagatgaaa
    26101 aataaaacgc tttccttatt gtgattttat tcactaatgc tgttaatatg cggattggca
    26161 taatttcctt tcatcgtgaa aatttgtgct tttagcttgt atttctcata aatccatgat
    26221 aatttattaa tgaagacggt taaatagtga tacagtttta ttcactgttt caatcgcaga
    26281 atgaagagat tttcttatag atgagagagg aatgtgtgac aaatggcaaa caaaaaagca
    26341 ccgatcgact tccaagcact gctagatgaa gtcaatgcag atttcccagt atatcaagca
    26401 cttgatcaag atggaaacgt agttaacaaa gatttagtac ctgatttatc cgatgatgaa
    26461 ctcgttgaat taatgactca tatggtatgg tctcgtgtat tagaccaacg ttcaacagcg
    26521 ctgaaccgcc aaggacgcct aggctttttt gcaccaactg ctggacaaga ggcaagtcag
    26581 ctggctagtg cttacgcatt tgataaagaa gatgttcttc ttccaggtta tcgggatgta
    26641 cctcaactag taaaacatgg tttaccttta tctcaagctt ttttatggtc tcgaggacat
    26701 gctgcaggta attcttatcc ggaagaattg aaagctctac caccacaaat tatcatcggt
    26761 gcacaatatg tacaagcagc cggtgttgca ttaggtttga agaaacgaaa caagaaaaat
    26821 gtcgtcttca cttatactgg tgatggcggt tcatcacaag gtgattttta cgaagcaatc
    26881 aatttcgctg gcgcttacca tgcaaatgcc gtattttata tccaaaacaa cgggtatgcg
    26941 atctctacac ctcgtgaaaa acaaactgca gcaaaaacac ttgcacaaaa agctgtcgca
    27001 gctgggattc ctggtattca agtcgatgga atggatccgc ttgcagtata tactgtatct
    27061 aaaatggcac gggaatgggc gatcagtgga aatggaccag tcctaatcga aacactgact
    27121 tatcgttatg gaccacatac actatctggt gacgatccaa cccgctaccg tacaaaagac
    27181 acagatgacg attggcagaa aaaagatcca ttgatccgct tccgtaaata tttgacggaa
    27241 aaaggacttt ggtctgaaga aaaagaagaa caagtcatcg aagcaactaa agaagaaatc
    27301 aaagcagcta ttgctgaagc cgacaaagta ccaaaacaaa aggtttcaga tttcttgaaa
    27361 aacatgttcg aagtatctcc acaatcaatc aaagaacaaa ttgcaattta tgaagcgaag
    27421 gagtcgaaat aatcatggca caaaaaacaa tgatccaagc aatcacagat gccttagcac
    27481 ttgaactaga aaatgacgag aatgtcgttg tttttggtga agatgttggg aaaaacggag
    27541 gagttttccg ggcaactgaa ggattgcagg aaaagttcgg agaagaccga gtattcgata
    27601 ctcctttagc tgaatctgga atcgcaggat tgagtttcgg tcttgcgtta gaaggatttc
    27661 gtccagttcc tgaaatccaa ttctttggtt ttattttcga agcaatggac gaagtagtag
    27721 ctcaaatggc tcgtacaaga tatcgaatga gcggcactag aaatctaccc atcacaattc
    27781 gttcgccatt tggtggcggt gttcatacac cagaattgca ttcggataat ttagaaggtt
    27841 tgatcgctca gtctcctggt attcgagtag tgattccttc caacccttat gatgcaaaag
    27901 gactattgat ttcttctatt agaagtaacg atccggttgt tttcctagaa cacatgaaac
    27961 tatatcgctc attccgagaa gaagtgccgg atgaagctta cgaagttcct ttggataaag
    28021 cagcagttac tcgtgaggga acagatgtat ctattatcac atacggagca atggtgcgtg
    28081 aagcaatcaa agcagcagac aatcttgcaa aagaaaatat atctgttgaa atcattgatc
    28141 ttcgtacagt tgcgccatta gatgtagaaa cgatcattca atccgtggaa aaaactggtc
    28201 gcgtagttgt tgtccaagaa gcacaacgac aagcgggcgt tgccgcgcaa gtagtttctg
    28261 agatctcaga acgcgctatc ctttcattag aagccccaat cggacgagtt tctgcacctg
    28321 atacagtctt cccatttgga caagcagaaa atgtctggtt gccaaatgca aaagatatcg
    28381 aagacaaagt aaaagaaatc gcggaatttt aagaaaagaa gggacgaaac aaagtcatgg
    28441 cttatcaatt taaatt
//
LOCUS       DARBEC010000027        28065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.027, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000027 DARBEC010000000
VERSION     DARBEC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.027"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(106..1005)
                     /locus_tag="SCL80_001138"
     CDS             complement(106..1005)
                     /locus_tag="SCL80_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEG1107020.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            complement(1015..1371)
                     /locus_tag="SCL80_001139"
     CDS             complement(1015..1371)
                     /locus_tag="SCL80_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1107021.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            1726..2022
                     /locus_tag="SCL80_001140"
     CDS             1726..2022
                     /locus_tag="SCL80_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEG1107022.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            complement(2666..3619)
                     /locus_tag="SCL80_001141"
     CDS             complement(2666..3619)
                     /locus_tag="SCL80_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1107023.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(3642..4397)
                     /locus_tag="SCL80_001142"
     CDS             complement(3642..4397)
                     /locus_tag="SCL80_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1107024.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            complement(4394..5353)
                     /locus_tag="SCL80_001143"
     CDS             complement(4394..5353)
                     /locus_tag="SCL80_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1107025.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            complement(5346..6296)
                     /locus_tag="SCL80_001144"
     CDS             complement(5346..6296)
                     /locus_tag="SCL80_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEG1107026.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            6446..6898
                     /locus_tag="SCL80_001145"
     CDS             6446..6898
                     /locus_tag="SCL80_001145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEG1107027.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDASILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            complement(6987..9254)
                     /gene="gshAB"
                     /locus_tag="SCL80_001146"
     CDS             complement(6987..9254)
                     /gene="gshAB"
                     /locus_tag="SCL80_001146"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEG1107028.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKEYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(9441..10439)
                     /gene="ftsY"
                     /locus_tag="SCL80_001147"
     CDS             complement(9441..10439)
                     /gene="ftsY"
                     /locus_tag="SCL80_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEG1107029.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            complement(10450..11259)
                     /locus_tag="SCL80_001148"
     CDS             complement(10450..11259)
                     /locus_tag="SCL80_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1107030.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            complement(11268..14849)
                     /gene="smc"
                     /locus_tag="SCL80_001149"
     CDS             complement(11268..14849)
                     /gene="smc"
                     /locus_tag="SCL80_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEG1107031.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIETALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            complement(14869..15555)
                     /locus_tag="SCL80_001150"
     CDS             complement(14869..15555)
                     /locus_tag="SCL80_001150"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEG1107032.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            complement(15903..17684)
                     /locus_tag="SCL80_001151"
     CDS             complement(15903..17684)
                     /locus_tag="SCL80_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1107033.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            complement(17710..18624)
                     /locus_tag="SCL80_001152"
     CDS             complement(17710..18624)
                     /locus_tag="SCL80_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107034.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMGTNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            complement(18651..19613)
                     /locus_tag="SCL80_001153"
     CDS             complement(18651..19613)
                     /locus_tag="SCL80_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107035.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            complement(19613..20557)
                     /locus_tag="SCL80_001154"
     CDS             complement(19613..20557)
                     /locus_tag="SCL80_001154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107036.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            complement(20558..21562)
                     /locus_tag="SCL80_001155"
     CDS             complement(20558..21562)
                     /locus_tag="SCL80_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107037.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            complement(21830..22072)
                     /gene="acpP"
                     /locus_tag="SCL80_001156"
     CDS             complement(21830..22072)
                     /gene="acpP"
                     /locus_tag="SCL80_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1107038.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            complement(22194..23195)
                     /gene="plsX"
                     /locus_tag="SCL80_001157"
     CDS             complement(22194..23195)
                     /gene="plsX"
                     /locus_tag="SCL80_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEG1107039.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            complement(23244..25280)
                     /gene="recG"
                     /locus_tag="SCL80_001158"
     CDS             complement(23244..25280)
                     /gene="recG"
                     /locus_tag="SCL80_001158"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEG1107040.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKQFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LITGSTKAKERRELLEKLEQGEINVVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            complement(25397..27079)
                     /locus_tag="SCL80_001159"
     CDS             complement(25397..27079)
                     /locus_tag="SCL80_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEG1107041.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKEIPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            complement(27131..27493)
                     /locus_tag="SCL80_001160"
     CDS             complement(27131..27493)
                     /locus_tag="SCL80_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1107042.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            27763..27951
                     /locus_tag="SCL80_001161"
     CDS             27763..27951
                     /locus_tag="SCL80_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEG1107043.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
BASE COUNT         7754 a         6070 c         4945 g         9296 t
ORIGIN      
        1 tattttccgc aaaaaaagca gcatatccac gataaatgga tctactgctc ttacttatcc
       61 ttatgtttta caaggtattt tcttttacac attactaatg ctgtctcaat acggaacgat
      121 tgtcatcgct tgttcaaatt cctcttgcaa atgttgtgtt tgataggcct gaaaagctgg
      181 gtgatgggca cgcaagagga cacgtaattt cataggatca tagctgtcta gcaactgttc
      241 tttcaagaca aaatctcggg tcagctgcca ttcataataa taaccaaaag gtctgcagct
      301 gtaaatcgtg tgaccgatgg atttatcttc aaagcgacga tgcgtatggc caaaaactac
      361 ttgtttgata tttgaataac ggtcaagtaa ttctccgaat gattctgatc ctaaaaatgc
      421 atttaatttg ttccaacgct cataaggagc agattgatac atgataaatg cccgtttagg
      481 aacgaaatga gtggataaaa tcacctcaag tttttttgct tccagctggt ctagcaaatt
      541 ttttgcagct gcaagcattt ctgcattgac ctcgagatct gttttcccac gctggatgaa
      601 ccgatcatac caatacaaac gcttcattcg tacgattgcc tcgtaatctt tcaattcgga
      661 aaactgatag tcgtaccagc cattcatccc taaaagaacg gtctgctcat ttaaagcaat
      721 ataccgttca tttaaaaaat gtgggtctgg aaaatggtcc atcatctgtt cattgggcac
      781 atctgccaac tcgtgattgc cgaaatgaaa tgtcgtagat aatcctttat tgacgaaaaa
      841 ttcgacaaca gctaggcaac ggtctacttt attagccgta tctccggcta gatgaagccg
      901 agtgatcttg tgcttttgca aaacttgcca aagctgcatc agctctgatt cgccgaattt
      961 attgatgtcc acatgtaggt cgctgatgat cgccaatttt cccattttat ccctctagtc
     1021 ttttcgaaca acttgcaagt tgccttttct caagccgctt ggtcctttta attgtaaact
     1081 attataagtt ggttctaaag caagtgtgga tggatcatcc atgtaaagta ttgcactgtc
     1141 tttgattttg attggtccaa ctggtgtatc agccactaca gaataaagac ttagatctgt
     1201 ttgtgtatct tttttgacaa tgatcaatcg aaatgccgta tccaattggc aactgacttg
     1261 gctttctgca aatggaccgt caccgttttc gatatctaat aaaagttgat cgtctgcttc
     1321 taatttattc ttcaatactt cttgtgcttc ttttgtcact tctaatttca tctgtatcac
     1381 ctcatgtcat agaataatca ctttattcat cagatgcaac taatttgtct ggttctgact
     1441 gttctcaatt tattggaaag taagtcattc gtttattatg atttctctta tcacaaaaga
     1501 gacaactttc ccttttcttc gttcagtttc ttgaacggaa aaagacccaa aaagcaacct
     1561 gttatttgtt taaccttatt cctttcttaa ataacaaaaa atactataaa aaataaaaaa
     1621 cctttaacaa ctattggaaa aagaattact ttttgttcac ggattattaa caactcttcg
     1681 gtagagtaaa cttatcaaat aaaagacttc tttggaggaa tcactatgaa ggaatatata
     1741 aaaaacatct attttatcga agaaacacaa aatatcgagg gttcttatat tgaagtaaaa
     1801 acactctttg tcaatgaaga taaaaccaaa gcattggata tttataaaaa attagcttca
     1861 aaaaaaacga atagttttgg attgatatta agcgagtata aaatcaaagc agaagagagt
     1921 tatttctatc aattattaaa acggtggagt aagcttcccg ccgattttta tcgaaaaatg
     1981 cagatcatca actatcaacc gcttgctgag acacatgcct aaatagaggt tgtgaaatca
     2041 gtgctttgag ctaataccga aggtcagctg attgaacatt gtttagaaga gattgtgaca
     2101 actgcgattt gctttgagta ataagaacgc tggacagaaa atagcttctc atattttcta
     2161 gtctagtggg cttattgccg agaagcagca gatggaacat cgtttagaag agattgtgac
     2221 aactgcgatt tgctttgagt aataagaacg ctggacagaa aatagcttct catattttct
     2281 agtctagtgg acttattgcc gagaagcagc agatggaaca tcgtttaaag aagagatcgg
     2341 tacaaaataa aaatatctaa atttaagttt tatctaagtt tcgctttgta cctttgattc
     2401 aaatcaaagg aggcgatctt gatgctgaaa aaatggatca cactattttt tgccactccc
     2461 cattctagaa tcaacccgta tgatgtagat aatgaattca cgttgaaaaa agatttctca
     2521 aataaataaa aaccatccgt aaaatcatca atacgatttt acggatggtt tttgataact
     2581 tctttgagga taggctcttg ctatttaaga atgtaagaaa cattattgtt acatagtatg
     2641 agagtttaat cctctgttat ttatgctatt taaatgcttg attcacatct tcgatcatca
     2701 gtttcattga ttctaaaccg cccccgctta aataccagac atctggttca agggagatga
     2761 tctgctgatt tttaccagca ttcgtttgtg ctaccagttc attggcagaa atatcatctt
     2821 tgctgtcgtc tccaccaata gcttttgttc tgtctactac aaataagata tccggatttt
     2881 tttccagcac atattcataa gaaacacttt gaccgtgagt ggatgcttcg atttgatcat
     2941 ctgcttgttc aaagccaaat aaatcatgga tgaaaccgaa tctagaacca gatccgtagg
     3001 ctgataattg cccttcattc acaagtgtga ctagtgcttt tttgtcagta gcttctgctt
     3061 tctttttcgt ctgatcgatt gcactagata attcctctaa ttgagtttta gcttcttctt
     3121 gcttaccaaa gatctcccct aaagcagtga cattttgctg gaaagattcc catggatttt
     3181 tcgcatctaa agaaaggaaa atggtcggag caatttgact tagatctttt tgaaaatctc
     3241 tttgtcggcc agaaatgatg attagatctg gctgaagctg attaattttt tctaagtctg
     3301 gttcttttat tccacctgct gattctactt ttttatagga tgaaagatac tcaggaaggc
     3361 tgcttgtagc tgctccaact accttatctc caacaccaag agcatccatc gtatctaaag
     3421 aaccattgtc gaatacgacg actttttctg gattatttgg cacctcgatt tggtctccat
     3481 tactatctgt tatcgtcaat gtagtttcag tttgtgtgct gctttttttt gtgtctgcct
     3541 gactggtgtt gttgctattt ccacatgctg ctaaaccaaa tgatgctaga atcaagatac
     3601 ctactactaa ttttttcatc tcttctctcc taatgttagt tttagaaata aagacagaat
     3661 ctgcggcctt caatctcaca aatccgtatg tccatttcgt ataattcatc caatacatct
     3721 ttttttatca tttcttgggt cgttccttgc gtaaataact gtccatcttt cattgcaaca
     3781 atttcgtctg cataacttgc agcaaagttg atatcatgaa gaacaataac aattgttttc
     3841 ccgatttctt gtactaaatt ttttaaaatc tgcatgattt gaaccgcaaa attcatatct
     3901 agattgttca aaggttcatc cagtaaaata taatccgtgt cttgcgctag caccattgca
     3961 atatacgcac gttggagttg accgcctgaa agagtgtcaa tcgattgatc cgctatttct
     4021 tctaacccaa ggtgttccag tgattcctga acgatttcct cgtcttttgt cgtcaatttt
     4081 cctttcgtat aaggaaatcg tccaaattct actaattctt tcacggtgat ttggacattt
     4141 atttcattcg attgtcgtag gatcgataat tttttggcta attcattgga tttccaagcc
     4201 tttacttcgt tatggtctaa atagatttca cctgtatctt ttggtatcaa acgactgatc
     4261 atcgacaaca ccgttgactt tccagcacca tttggaccga taaaagcagt caatttcttt
     4321 tcaggaatcg ttaactgtac atctttaacc actgattttc catggtacag tttattgacc
     4381 ttttgtaaat caatcatagg atactcttcc tttcgcgaat caattttgtg acaaaataaa
     4441 ttcctccgac aaattcaacg acgacactca ctgttgtatt ccagccgaaa atctgctcta
     4501 ctgcgagctg tcctcctgtc agaaacagca tagctaccag aaagcttccg ataaatagct
     4561 gaccatgacg ataagtagaa aagacacgat aactgatatt tgcgacaata aaccctaaaa
     4621 aagtaactgg acccacaaga gccgttgatg tccctgtcaa aagactgaca caacataaaa
     4681 tcaaaaattc aaaacgatct gtgtctatcc cgaggtttac tgcttgttca tttccgagat
     4741 ggaaaacgtc tagcacatgt cgatatcgaa ataacaggta ccagcagccg ataatgatca
     4801 aagaagcagg caaaagatac tgactctgga tattgccaaa gcttgcaaat aatctgcctt
     4861 gaagcaaatc atattcattc ggatccatga tgacttgtaa aaaggtactg acactaccaa
     4921 aaagcgtacc taaaatcatc cctgacatca gtaataaaaa aagattcgct ctcatttttt
     4981 tcaaaagaaa gaaaaataac aatagactca gcccgaccat cagcccaacg ttcaataaga
     5041 aaaaaactgt acctgtctgc tgaagcatcg tcactcctcc gataaagaag aaaaggaccg
     5101 tttggacaag gacatataac gactcaagtc ccaatactga aggagtcaaa aactggttat
     5161 gcgtaattgt ctgaaagctg attgtggcac tgctgacagc tagcgagaca aggataaatg
     5221 caaaaatttt cttactcctc aatgcacatg caaaatgcca gtttccatat gtatggatcg
     5281 tcaagtacat tcctgtaaca aataaaacaa gaacaagcat accaataaaa atagatggct
     5341 tttttttaag catatcgtcg tccccttatc agaagataaa ggaagatagc cccaccgatg
     5401 attccaacga tcacactgac tggtatttca taaggaaaaa tcaacacgcg agcgacaaga
     5461 tcgcaaaata ctaagaacaa actacctgct aaagcagtta cgccaattgt tttgtgcact
     5521 tgatctccat atattctaga gacaagattc ggaatgatca ctccgataaa aggaatgctt
     5581 cctaccatga tcaacatgac tgcactagcc aatgatacta agccaatgcc taaaaactgc
     5641 atcttctggt aatctattcc cagatttact gcaaacgatt cgcccagacc aatgatcgta
     5701 aatcggtaag caaatagcca aaccaagagc aacaatggaa caacagcata caataactca
     5761 taatttcctc gtatcacagt cgcaaaattc ccttgaagcc atgaagtcat attttgtacc
     5821 aactggaatt gataagcaaa aaaagtagca actgaaccaa tgatattccc taacatgact
     5881 ccaatcaacg gaagcatcaa ttgatttttc tggaggaaca atcgcgtaag ggataaaaaa
     5941 agcaatgttc ctgcaaaagc aaagcaaaat gctgtcaagg atcggacaat caaggatgca
     6001 ccaggtagga aaatcatagc cactaaaata cctaaacgcg cactatccat cgtgcccgca
     6061 gtcgttggtg aaacaaattt attttgggtc aaatgttgca tgatcaaccc acaaatacta
     6121 ctggtcgctc ccacaaggat caaacaaatc gttcgtggca aacgagtcac tagcaagatt
     6181 tgtaagcttt gcccatctaa ctggagcagt tctgacaatt gtacattcga tactccgatg
     6241 aacaatgaaa tactacttaa aataactaat aatgctacta gtcgtccgta gcgcatccaa
     6301 tccacctttc taattgagaa tgattttcat tcttatttta agtgaatcgt tgaacaaaag
     6361 caaccgcttt ttcatatgtt ttaggtatta ttgggaatat gctataatcg taagaaagac
     6421 aaagagaaga aggagggtat tttttatggc acaagatgat gtaaaaagcc atttggataa
     6481 gggactttat ggtacacctt tagtgaaccc tgaagaacag cataaatatt tagggacatt
     6541 cagggaacgc tgctatctta gtatgactgt tgctgaaatg atccaaccaa aaaacaaaga
     6601 aaacttttta aaagaactca aaaaacatcc agatgcttcc atactcttga atggggctct
     6661 acccctttct gtccaaaacc aatatatcca gctagcaacg aaaaacaata gccgtttcac
     6721 tgtagtcaac gattacgtca ctgcaaatcc tgaggaattc ggattgctct taacagcaaa
     6781 agaagcagta aatgaacctg tgattgatat cgaaaaaaaa tacccagaag aaaactcttc
     6841 gccagctgaa aaagaaaaac caaaaaagaa aaaaagcttc tgggatcgtt tattttaaaa
     6901 cgaaaaaaca cataaatttt tacatgcaaa aaaagctttt gatcagattg acatcaaata
     6961 ggacaatctg tcaaaagctt tttcttttaa aatacttcag gatataacaa tttcaataca
     7021 ttcatcgtca atcttctgcc ttttcctgca aaaggataaa catgcatatg catggctgga
     7081 ttgaaatttg cttcgatgat cccatatgct tttttatcag ttgtaggatc gatttcttta
     7141 tctggtataa tcaaatcgat accactaatc ttagctccta gtgcttctac cgcagaaacg
     7201 gcgatttttt tgtaagcttc agaaaattca tcagtcatat cgatcgaatc accacctgta
     7261 ctgatatttg agttctccct tagataaaca atttgattgg cttggggaac tgattcgatc
     7321 gtcaaccctt gttctttcaa catcaattgt tctaattcac ctaattggat caattctaaa
     7381 ggtgcacgat gattcgttcc acgcaaaggg tcactatttt tttcttcaac aagttctttc
     7441 actgttcgga tactgtcacc aataacatta gcagggactc gcaacatgat ggcttggact
     7501 tctccgtcga tcacaaagaa acgatattcc gttcccggca taaattcttc tactaaaacc
     7561 gaactgtctt cacgaaaagc aatggctaaa cctgcttgat aatcttccag actagctcct
     7621 tccttgaaaa tcgtgatacc taacccgtaa ttggttgatt taggtttgat cacaaatgcc
     7681 tgctcggcaa atctcggata agcttttacc gcttcttcca tggatgaaaa ttctgcgcct
     7741 cctggtaccc ggaacccagc ttcttttaag acttttttcg ttactgtttt gttttccatg
     7801 ataagtggga cgatatagct gtccttactt gtcatgtttg catttttcac atattctaca
     7861 tggtcttgga attgcagacg caaaaattga tccgactcat ctaatacttt gacttggaca
     7921 cctttttgga tggcatcgaa cataaatatc tgagtagaaa gttccatttc gcgaaaaccg
     7981 gccaactgat agggacgttc atggctttct ttataatatt gatgtcccaa tgccacagca
     8041 acttcttgct gactcgatag ctgtgcttct tgatagagtt tcgctgctaa cgtttgttca
     8101 ggatgatcca tttgagtcaa tgcttcctct actaaataaa gagaatccaa ccaatccaat
     8161 tcaaccagca tttgcttcat tccttcaaga atttctctac cttcttgatg aaattctgtt
     8221 tgatctgatg gttgttctag tgctactttg ttatttcgag tcgtaccttc tgctacccat
     8281 tggtcacagt cttctttttc ttctgtccat aacatgtaca tcaggaataa ttgtaagaac
     8341 cgaacttgct ctggattgat acccaatctt gcataaggat tcaaatcgat attccgcaat
     8401 tcaatataac gaacacctgt tgttgctaaa tctgcgactt tctttccgcc tcggaaacga
     8461 agcggtgtat aaaattcttt ctcctccgaa agtttccctt cttcgatcat tcgctcgata
     8521 tctgctaaat actgtttcat agaagcatag gaaactttca catttggatg attcgtatag
     8581 cccagcgcac tgttgcgaat actgcgaaca ggttcttgcg gatgtgattc atctaaaaag
     8641 tagttcttct cactcattgg tgaagcaccg aataaataag tgatcatcca gcgatagcgc
     8701 ataaagtttc tcgctgtttt caaataaagt tctgttttga attcggaaaa atcttgaaat
     8761 tcttcctgat ggctgaacaa tgttcttagc aactcatcgc caaattcaaa attgaaatga
     8821 ataccgctta ccatctgttt tcgcttcccg tattcttttg ctaaataacg tcgatacaag
     8881 acatcttcga aattttttaa ttttgcaata atgatctctt cgtctttttc cggtaaggca
     8941 ggtggcatac ttaatggcca gatcatctct tctttcggta tagaacgagc agtcacatca
     9001 taaacagcag cgagatactg aaataattcg ggaatagaat cggtaacagg ggtgatcatc
     9061 tctgtttgtg tttcgctaaa atctgtttgg atataggggt gataggatcg atcgccaaag
     9121 attgctggat gatcggtttt tgctagattt cctgcaagat caacccgctg tccttcccgt
     9181 tctataccaa atcgagcttg gtcgataaaa gggcgcgcat tgacatgcaa taataattgc
     9241 ttaaaattca tcattaagtg acctccaatt catatgaatc taattataat caaaaagtct
     9301 aagaaaaaga acgaaattgt ttgagaaccg cttacaaata agtataaaaa tgaaggcatc
     9361 aaaaacacag ctatgttaaa aacaatcatt caaacaagga gctgcaactt ttgtcacagc
     9421 tcctgctttt tagacatggg ttattcttct tttagtaatc ctttgaacaa tccaacgaca
     9481 aaatcatttg gatcaaacgg ctccaagtca tcgattcctt caccaagacc gaccaatttc
     9541 actggtaaat gcaattcatt acggatagca agtacgatac cgccctttgc tgtaccatct
     9601 aattttgtca aaacaagacc ggtcacatct gttgtttctt tgaattgttt tgcttgaacc
     9661 atcgcgtttt gaccagtggt tgcatctaat acaagaagtg tttcttgggg tgcttctggt
     9721 aattcccgct ggattactcg ttttattttt tctaattcgt tcatcaagtt cactttattt
     9781 tgcagtcggc ctgcagtatc aactagtaaa acatctgcct gctctgtctt tgctctttcc
     9841 aaagcatcga atacgacagc tgctggatcg cctcctgcat tgccacgaac aacttcaaca
     9901 ccagcgcgtt cgccccagac aactaactgg tcgattgctc cagcgcggaa ggtatctgct
     9961 gctgccatca atactttttt accttcgttt ttgaactgat gggcaagttt accaatactt
    10021 gttgttttcc ctacaccatt aacaccgaca aacaaaataa ctgttagtcc gtcttcttgg
    10081 atatttagtt catttacttc attgattcct tccgcttcat agagatccac aagtttctcg
    10141 atgatcgcat tttggacttc tgccgatttt ttcgcatttc tgagtttcac ttcttgacgt
    10201 aatgcttcgg taattcgtat cgctgtttca aaacctacat ctgcaccgat aagtgtttct
    10261 tctaattctt caaaaaagtc ttcatcgact gttcgaaaat tggcaaacaa ctcattcatc
    10321 cgttcaccaa atgttttacg cgtcttttct aaacccttgt cgtacttctc ctgtacttca
    10381 acttcttctg ttttctcttc gccagtaaaa gcacgcttga ttttatcaaa aaatcccatt
    10441 tgactccccc tactctaata caaatttttg gatcactttg gctacgccat tttcttcatt
    10501 gctggctgtc acgaaatctg ctgcttcttt gacgaaaggc acagcattct ccatcgctac
    10561 gcctagtcca gcatattgga tcatcggtaa atcattttcc tcatcaccga tcgccatgat
    10621 ttcttcttgt ttcaaaccaa gatcttttgc caataaagaa ataccataag cttttgtaac
    10681 accttttggc ataaattcca aaagcatact tcttgttttg atgatctcat acatttcatg
    10741 gaattctgct ggtatttctt tgattttagg atctaattct tcttggtcat aagcgattac
    10801 tgccttatta agcacaaatt catctgtaat atcgcttata tccgctggtt ggaattgcag
    10861 cagcttattc aacacattgt acaaggattg cttccttggg gcactaggca gctgtaaaac
    10921 cacattgtca gacaaaacat ctagtggcag atccaattgc tgagccagtt tgtataagcg
    10981 ttggatatct gatacttcca tcactttctt ttccatgatt tcaccagtat catttttttg
    11041 taccaaaccg ccgttgaaag tgatgctgta gtcaccttca tctaccagtc ctaattcttg
    11101 cagataatgc gccatcgcag ctaaggggcg tcctgtacat agaacgatct tgaccccttg
    11161 ctgttttgct tgtgctaatg cttgtttatt ctcatctgaa atcagctttt gttcatttaa
    11221 taatgtaccg tccaaatcga ttgctactaa tttgatcatt ttcgttccta cactttcgtt
    11281 tcgttgattt tcgcctcttc attgatatct tccatccgta cagaaacgat ttttgataca
    11341 ccggattcct gcatcgtcac tccgtacagt acatctgctg cgaccatcgt tcctttgcgg
    11401 tgtgtgacta cgataaattg tgtgtcatct tggaaatcgc tcaagtaacg accaaaacgt
    11461 ttcacgttgg cttcatctaa cgctgcttct acctcatcta agatacaaaa cggaacagga
    11521 cagacacgga taatggaaaa gagcaacgcg atggctgtaa gtgctcgttc tcctccagaa
    11581 agcaaactta gactttgcag ttttttgcca ggaggttgta cttcaatctc aatacctgtt
    11641 ttcagcaaat cagttggttc cgtcagtact aattcagctt taccgccgcc aaacatattt
    11701 ggaaagacaa ttttgaattc ttgacggata gcttcaaata cttctttgaa tcttgtccgc
    11761 acttcatcat ccatctcatc catcgtttca aacaattggt ttttcgctgc taacagatca
    11821 tctcgctgtg ttgctaagaa tgtatgtctt tcactgactt gttcgtattg ttcgattgaa
    11881 tttaagttta ctggtcccag tttttcaatc tgttctttta aggatgaaac ctttgttcta
    11941 ctgtcttcga tatcggttgt ttcttgatag tcttgggaag ctttctcaaa tgtcagctga
    12001 tattctgact gcagataggt caaatgatta tctaaaatca tttcagctcg gtctttttgg
    12061 acttctaatt tggattgttc cgacagtcgt gctttttgct cacgattacg ctctgccaag
    12121 actgcttcaa gcttatcgat ctccttttgc cttctatctc gctgctcttt ggcttttgcc
    12181 aactcggctt ttaatgtttc tcgctgagca gataattcgt taatttgttt ttccaaactt
    12241 tcttctgtga cttcatgatc agaaaagtct gctgtcaatg tcgccaactg cttttctatc
    12301 gcttcttgac gttcggttgc ctctgctttt tggactcggg caccgcggag ctggatctgc
    12361 agatgattaa actgctcttt caagacagca agatctgctt gttcttgtgc tttttgactt
    12421 tgtacttgtg ctcttctggc ttccatctgg tcgctttctt gacttaacga tttgatttct
    12481 tcgtcgattt tttgtcgttg cgattcaaga tctgtttgtt tgtctttcaa ttcgttcgtt
    12541 tgcttttcgt aatcttcgat aaactgctgc agttcacgtg tctcaaaagt tgagatttgc
    12601 ttttcttttt caaaacgttc aagttcattt atgatgtttt gtagctgatt tgtcgcttct
    12661 tgttcttcaa aacgtaattg ttcgcctcgt gtacgcaaaa cttcttgttc ttcggctaaa
    12721 cgagctgttt cttgttgcag ctcctgaact tttttttctt gtgcttggag ttgtttatct
    12781 gcttcttcaa attcactagt caactgtttc aattcttgat tttgtacaaa caaactgcct
    12841 tggtttcctc gtttattcgc tccacctgtc atggatcctc cggcattcat cacatcacct
    12901 tctaaggaaa ccactcgata ttgatagcgg atcgtttgcg cgattgcatt ggcactagtt
    12961 aagtctttgg ctaaaaggat cgttcctagc aggttttgaa ccacagtttg gatttgatcg
    13021 ggataactga cttgttcact ggcaatccct ataaaccctt ctacagctgc tgcttgtgtc
    13081 aatatatgag ccggtaattg tcttggtttg attgtcgtca gtggaaggaa tgttgctctt
    13141 cctcctcttt gctgtttcaa atacgtgatg gcttgccgag catctttttc attttcaacg
    13201 atgacatgct gggctgcccc acctaaagct gtttcgatag ctaacgtgaa atctgcagga
    13261 acatcgatca gttcagccac tgctccgaca atacccgaaa gctgctgttt atgctgcaag
    13321 accaatcgaa ccccttggta aaaaccaaaa tagttttctt ggatttcttg aaggcttttc
    13381 ttacgagcac gtagctgctg gacttgattc attaattgat acattttagg ctgttctttt
    13441 gctaatgctt cttggaactt tttcccgttt tgttggatca tttctaactt ctcttttgtt
    13501 tcagttaaag atgattttag ctgcgcctgc ttttgtgtca attcttcttt ttgtaagatc
    13561 aatcggtcta ctgatgcttc tacttccgat tgcttggcta acgtctgctt gctttttgct
    13621 gtttcttgta tatattgacg ttctagatac ttcagttcgt ttcctacagc tgcttgttct
    13681 tgcatcaaat caacatattg atctcttaat tctgccaata attctttcgt tgatttttgg
    13741 tatttttcta attcttgctg tgtttttatg agatttgctt cagctttttg gatctctgtt
    13801 tctttttctg ctgctgcttt catcaagctt tcttgaagct tttcaaaatg tttaaccttt
    13861 ttttgtgctt ctgctaatga agtttgatat tcttgcgagc ttttttgtgt atgctttgtc
    13921 cgctcctgca aaacatcttt ttgtccttct gtctgcttca gtttttccga aagatctaat
    13981 aatacttgct gatttttttc gatcagacga tcggcttgcg cattttcttt acgctgcttc
    14041 gccaaaatgg attcttgttc ttggatcgac tcagatagtt tgcctaattc tagattgaat
    14101 ttcgcaagct gtgcttgttt gttgtcccaa tctttttttg ctgtcttgat ttcagcaacc
    14161 atcaaagaga catcggtttg agtcagtgtt tcttttagcc gtaaaaactc ctttgctgcc
    14221 tcactctggg cggccaaagg agttaactgt tcttccaatt cgtggatgat gtcttggacg
    14281 cggctgaggt tgtcttctgt ttcaaaaagc ttctgttctg ctttcttttt ccgctgcttg
    14341 tatttcaaga caccagctgc ttcttcaaag atcccacgac ggtcttccgg tttactactg
    14401 aaaatcgctt ctacttttcc ttgagaaatg attgagaacg attcttttcc taatccagaa
    14461 tctaagaaca attcttggat atcttttaat ctgcagcttt gtttattgat gaaaaactcg
    14521 ctttccccag tccgtcgata acggcgggtt acactgatct cattgtactc caatggcaga
    14581 taatgatcac tattgtctaa aatcactgtg acttctgcaa tattcagctg cttacgggta
    14641 tcggaaccgg caaagatgat atccggcatt tttccgcctc gcagactttt agcagattgt
    14701 tctcctaata cccagcggat agcctctgta atattgcttt ttccgctgcc atttggtcca
    14761 accactgctg tgacgctatt ttcaaaatca atgactgtct tgtctgcaaa tgatttaaaa
    14821 ccggctattt caattcgttt caaatacaaa gctttccctc acttactttt acagtgctgc
    14881 caatgcattt tccgccgcag cttgttctgc taattttttc gatttccctt gtccagtgcc
    14941 aatcaattga tcatctacat aaacttcgat ccaaaatact cgttcatgag ctggaccttc
    15001 ttcattgatc aatcggtatt cgattgatac atcacctgat ttttgcaaga cttcttgtag
    15061 ttttgtttta tgatccatct catgtgaaaa agcacctgct ttgactttag gaaaaatcac
    15121 tttttcgatg aatgcatggg tcgtgtcgaa tccctggtca agatataacg cgcctaaaaa
    15181 agcttcgaat aggtcgcaaa gcaatgctgg tcgcgtacgt cctcctgaat tctcttctcc
    15241 cttgcccaac aaaacatatt gatcaaagtg acattctttg gcaaatttcg acaagctgtc
    15301 ttcgcgaacg atcgcagctc gtgtttttgt caattttcct tctggcaaat gaggatataa
    15361 acggaataaa tactgagaaa ccatcagttc taaaacggca tctcctaaaa attcaagtcg
    15421 ctcattatcc gataattgca gattgcgatg ctcattcaca taggatgaat gggtaaaagc
    15481 ttgttcaagc aaatttagat cgtgaaaaac gatgttatag ttttctttta gttcttttgt
    15541 cagctggttg tccattttcc acattccttt tatggatatt ttttcgatac ttccttatta
    15601 tactggtttt ttgtcaaatt tgaaatggtt cggtcaaaag gtaagtaaag ctttaataat
    15661 cctagtgatt gacgagaagt tgtgaaaaat tcgtttagtt tcgagtatga agaaaaaatt
    15721 ttgtgaaaat ggctttccat atttttgcat aaatttgact taataccgag aaactggact
    15781 ttgaacaccg tttattggag aagtcatgaa atcagcgcct tgacctgaat attagagaaa
    15841 taaacagaaa aatatcaaaa aaccgaaaaa ggacatcgcc ctttttcggt catttttagt
    15901 gattatgaac gtgaatctgc tgtaacagaa acatttgccc atgggtttgg tacatcataa
    15961 gaccaatccc agtctttgac acggtcgttg actggcaata cttcattacg gaacaacgtt
    16021 ggaatcgcaa atgcttcttc aaatgcatat tgttgccatt tttgatatgc ttctttttgg
    16081 tactcttcat caaatgcttt gcttgaactg atatcgttga tcaattttgt attttcttct
    16141 gattcataac gagtgtagtt gaacgcagca tttggtcccc atagtccact tggatttgga
    16201 tcagttcctg tattccaagc agcttggtaa acatcaattt caggatcatc gttttctaat
    16261 ttatcataga aagaattgaa ttcgatcaga cgacctgttg tgtattggac atccaaacca
    16321 atttctttcc attgttggat atagtaatct gccaatggtt gcgctgtttc cccaccggac
    16381 atcgaagcaa agttgattgt tagtttattt ccatctttgt cttctcttaa accatcgtca
    16441 tccacatctt tgtagccagc atcatctaat agttttttcg ctttgtctag atcttgtgtg
    16501 aatcctttca tctcatcatc atggtatgac tggaatactg gaggaatcaa tgaagtcgca
    16561 ttgcttctca aacctgcata gaaacgttca cctactgcat tattatctaa tgcatagccc
    16621 attgcttgac gtaatgattt atcagccatt tttgaatctg gattgtattc cacactcttt
    16681 gtatctgcat tccatttacc taacttgaat cctagataag tataagaagt ttgttcacgg
    16741 cctaagtttg tatagccagc aatttcttta taggtatcgt aagtatctgt tggcatttgg
    16801 aaaatcatat catataattt agagttcaat gcttcagttg cacttgccat cggtacagat
    16861 ttcatcgtga tctttttcaa tttaggcgta ccattccagt agtgctcgtt tggcaagtat
    16921 tctactgatt ctcctgcaac gatattactc atgtaataag ggccaaaagt cactggattt
    16981 ttacgtactt gatcgcttga ttccatatct ttgattgcta caccttctaa cgtatgtttt
    17041 ggtgctgcat atgaccagat accaccaccc gattgaagca tgcttgggtt tacttcttta
    17101 tattcgattt caactgtttt gtcgtctatt tttttgattc cggagattgt gtcggcactt
    17161 ccatcatgat attcttccat acctacgatg ttacggaaag tgtcgtcata acggatacca
    17221 gtgtaatctt tattcccaat gatttcgtaa gggaaaatca catcgtccgc cgttacatct
    17281 tcaccatctg accatttgac gttgtctttt actttgattg tcgctttttt cgtatcttca
    17341 tctagatcaa gcgttgcagc tccgtcgtct gtgattctga aatcgtcatc gtttgcaaat
    17401 agcggttcat gagaaggagc cataaaagct gcatcatatg aatcttggta aaactcccac
    17461 tggaacaatc ctttaaactg actgtccgtt gcgaccgcag cttctaattc gccgtcttcg
    17521 attgcatcat cattattttt tgttgcaatt ggaaaagtta attcctcttt gttattgccg
    17581 ttgcctccag agttgtttcc agaggtatct gtacctccgc ccccgcatgc tgcaagaacg
    17641 acagcagaca tcagcgctgc aaatccaaaa atcgttttct tcattcctat ttcccccttc
    17701 gcttctatac tagcctaacc gttggcgcgc atctgcagaa cgcttgaatg cttgtccaac
    17761 ataattgata ctcaacatca aaactaaaat aaggattgat gcgggtaacc agatccattg
    17821 ccgattcact aagacttctc cgttacttgc ataaccgatc aatgtaccta aactaggtac
    17881 atttgccggc aagccgaaac ctaagaaagt caaagttgtc tcgataccga tgtttgccgc
    17941 aaagttcatc gtcaaattag taatgattaa tgaacttaga ttcggcatga tttcgcggaa
    18001 catgatttta aaatcattgg ttcccattgt ttttgaggca ctgacataat ctcttcttgc
    18061 ttcagataat gtcttgcttc ggaataatcg ggctttagct acccaataaa atgcactcat
    18121 gatgaatata aaggaccaga cattgtatct ggaaataatc gatacgaaga cgataatgat
    18181 cagcatgatc ggtagaatca tgataaaatc aacgatccgc atcaagatgt tgtcaatgat
    18241 ccctccgtag tagccagaga tgatccctaa tccaaccccg atgatcgaag taaggatcgt
    18301 gatcgcaaat ccgatcgtaa cagagtttct agcacctatg atcaattgtc ctaatacatc
    18361 acgtccgcct tcatctgctc ccagaatgaa cttttgtcct tgtgtcgaaa ctgttcctgg
    18421 tgcagcgtat ttgtcgaaga tactaacata catcactttg tcttgatcgg tcagcatcgc
    18481 accgataaag atcactaata ataaaaccac tagcaaacct aatgaaaaaa tagcaatttt
    18541 atctttttta aattctcgag caatcattcg tatccccata ggaggaatac tttcttgaac
    18601 tgctgtattg tttttttcac tcatcgatcc attctccttt ctgattggtt ttattgaatc
    18661 cgaatacgcg ggtcaacgat actcatgatg atatctgaaa gtaatgtccc cactagcgtc
    18721 gcgattccta aaatcaatac taaggatgtg atcacggtat agtcccgttg gccgatggaa
    18781 tcaataaata gttttccgac tcccggataa ccaaaaatct tttcgataac tacagaacca
    18841 gcaatcaacg aagtgatttc ataccccatt tgagaagcca tcggcaacgc tgcatttcgg
    18901 aaaatatgtc gactgaatac tttattcgtc ggtacccctt tggaacgggc tgttctgaca
    18961 aaatcgagcg atttcgaatc gattacttca cttcgcagat attggattgt gacggcagtc
    19021 gccaaaaatg cttgggtaat tgatggtaac agaatatatt tcaaacggtc tatccattga
    19081 gtgataaatc caccttgcaa acctagtgtt tgtgatccac ttgttgggaa ccagcctaaa
    19141 cgataaccaa aaacaaatag catgatcaag ccgaagataa ataaaggaac agcaaaacta
    19201 aagaagttgt agattacgac aatcttatcc aagattgaat tttgataccg gccagcaagc
    19261 atccccagag gcacagcaat caaataagta aggacaactg tcaagatcga tagatataaa
    19321 gtgttgatga tccgttcgcc gatcaagtca gcaacagagc gtttgtataa gaaactttga
    19381 ccaaaatcgc cttgcaaagc atttccgatc caacggaagt actggacata ccatgggtca
    19441 ttcaagccag ctgcttgtcg catctgttcg atcactgccg gatcctgatt tggattgatc
    19501 aaaccggtaa atggatcccc cggcatcgct tgggctaata agaagatcag aacacttaaa
    19561 ataataactt gcgggatcat caataaaaca cgtcgcagaa tcgttttcca cattagcttg
    19621 cacctccatc tttcaacgcc actttgtgtg tagttgtgat cgtacgcaaa tcgtagactc
    19681 gaccagtcgc atcatagtat tctttttggt tttggatata ctcttgttct actcttcgtc
    19741 gattttcttt gtggctttcc ctattttcta catcgatttg cggaatagct gaaagcagac
    19801 gtttcgtata gatatgtcgc gggtcattat aaatgtcttc tcttgtaccg atttctacaa
    19861 atcgaccttt gtacataatt gcgatattgt cgcacatgtg ttttactaca cctaaatcat
    19921 gtgaaatgaa taaatagctg aggccgtatt cttgctggat tcgtttcatg aaattcaata
    19981 cttgcgcttg aacagataga tccagagcag atacaggttc atcggctacg atcagttttg
    20041 gattggtagc aacagcacgg gctactccta gacgttgacg ttgtccgcct gaaaattcat
    20101 gaggatattt atatagcgca tcttccggca tcccaacgat atctagcagt ccttttacct
    20161 ttttcttttc ttcttgatcg cttaaccgtt caaaatttcg gatgggttca gcaatgatat
    20221 ccaacactcg ctttttagga ttcaaactgg acattgaatc ttggaagatc atctgcacat
    20281 ccttgttata acccattttt ttgcggttgc ttcgcttcgt tacatctttt ccttcataca
    20341 tgatttgtcc ggctgttacc ttttctaatc ccacaacggc ttttcccgta gtagattttc
    20401 cagagccaga ctctcccact aaaccatatg tctttccttt ttcgataata aaatccacac
    20461 catctacagc atatacatga tccgttactc gattgaagaa tccactgcga atagggtaat
    20521 gaactttcag gtctttgatt gtgattaatt ctgtcatcta cacctctcct ttttcgtctc
    20581 taaagtggaa atgcttatag caggagcaac gtaccaagtg gttcggtgcg acttcatgaa
    20641 ggataggctc ttcttcatga tcgcttgctg gtatccatgg aatacgtggt gcaaaccgac
    20701 aacctttacg aggcatgttt ttcagagaag gaacgacacc ttcgatcaca tggagttctg
    20761 catcatgatt tccttcttgt ggtatagagt tcagtaaaga gcgtgtataa ggatgcttag
    20821 gatgatcaaa cagttctttt acacctgcta cttcaacgaa ctgtccgcca tacatcactg
    20881 ccactcgatc agccatttct gctacgactc ctaagtcatg agtgatcaag atgatccctg
    20941 tttttgtttc ttcctgaagg tctttcagta aatcaagaat ctgtgcttgg atggttacat
    21001 ctagggctgt cgtcggttca tctgcaatga cgattggagg tttgcaggcg attgcgattg
    21061 cgatgatgac acgttgtctc ataccgcctg atagttcatg gggatattgg cgtccaactc
    21121 tcgcaggatt aggaatccct acttgttcta gcaattctag tgcacgagct tgacgctgtt
    21181 ctgcattcat ctttgtgtga taggaaagtc cttctttgat ctggtcttcg attctcatga
    21241 gaggatttaa tgcagacaac ggatcttgga aaatcattcc gatatcattt ccgcggattt
    21301 tattatagag cgtttcgttc aggtcgatta aattcagatc gttatataaa atctcacctg
    21361 taatttttgt gttattagga tcaagtaatc ccataatcgt agtagccaag gtacttttcc
    21421 cgcacccaga ttccccaacg attgctaaaa tctcgttttt gtctaaagta aatgaaacgt
    21481 catctactgc atcgtaatag tcatctttga gtcggaatcc tgtatgtagg ttctgaactt
    21541 ctaataattg gttatcggac aatcgaatac ccctctctgc ttcctaaatg gaagtttttc
    21601 ttaataaaaa aggacattac acagaataaa tcgttttata acaaaggtta ttcttatctt
    21661 tgtgtaatga attattaata attatataga aaaccgcttt actttgcaac ataatttctg
    21721 tttatcgcca gaattatcag aaaaatgact tttacgtgtc aaaaagatta aaaaaagaag
    21781 gataaaaagc tgcatgcagc tttttatcct tctttgcttt tatttgaaat tatttaagat
    21841 ggctgctgat ataatctact gcgccaccga ctgtttcaat ttgttcagcg tcttcatctg
    21901 aaatttccgt accgaattca tcttcaagct ctaaaacgaa ttccataata ctaattgaat
    21961 cagcttttaa atcatctttg atgttcaatt catccgtcac tttatcggta tctatctcaa
    22021 aatgatttga aataattttc gctactttgt taaatacttc ttcgcgagtc aactttttca
    22081 cctccaagaa tcatacactt gtcaattacc tacaccatgg ctaattttac tggcattttt
    22141 caggaatgtc cacctttttt tcaatggaac ggatcgcttc agatatccaa atactattct
    22201 tcgctttttt caaattgttc gactaattgt ccaactacat ctgtctcaag cattgtgtgg
    22261 atttggcgga tcgtatagcg aacagcatcg gggcctgttg ctccatgagt tttaatgact
    22321 ggtgctttca agccaaacaa aacggcaccg ccatgtttgg aataatccat ttctgctttc
    22381 aaagaacgta agccgtcttt taaaagcata gcgcccattt ttcctttgat cccttcattt
    22441 agaatagcag attttaatag attcatcata ttcattgctg ttccttcaat agatttcaaa
    22501 acagcgttgc ctgtaaatcc atctgtgacc actacatcag ccacaccatt gagtagctca
    22561 cgagcttcta cgtttccaat aaaattcaag cttgtttcat tctgaaggag ttcataggct
    22621 tttttcgtca cttcactacc ttttgttgct tctgttccgt tgtttaagag tgcgacacga
    22681 ggacgtccga tacctcggac tttacgtgca taaaaagaac ctaaaatacc gtattgcaac
    22741 aaatgttctg gtttgttttc tgcgttagcg ccaagatcca gcatatcgaa accggcacct
    22801 tctttcccta ctactggcaa ggtagacatc aagccagggc gttcgatatt tttgatacgt
    22861 cctacgatga acaaccctgc tgctagtagc gcgcctgtat tccctgctga aaatacagca
    22921 tctgcttcac cgtttttcac tgcttgtgca gcaaggacca ttgatgcagt cttttttcgt
    22981 cgaattgctt ttactggctc atcatcacta ttgatttttt cgtctgtatg gataatcgtg
    23041 atgttttttt catttgttaa gtatttcttg atttctgctt cttttccgta aagtaagaat
    23101 tctacttctg gaaaatcttg ctgcgctaaa gccacgcctt ctacaattgc ttgaggtgcg
    23161 tgatctccgc ccattgcatc tacggcaatt ttcattgttt ccactccttt ttccgtcatc
    23221 agtataaagt atagcacagt ctgctaatcg aaataacgat tatcctgatt ttctatttgg
    23281 aggctttgcg ctaaaggctg atactcagga agcatctgcc aatcctttat ttgccaaagg
    23341 gctgcagcct cttctctggc tacctccaaa acatttccat ccgtcaccaa gtcagcaatc
    23401 gcaaattgag gcagaccaga ttgacggaag ccaaatactt caccaggacc acgtaattcc
    23461 agatcttttt cgcttagtac aaaaccattg gtggtctctg tcatgatttt catccgttcc
    23521 acacctagtt catttttagg attagcaact aagatacagt aggaagcatc acttccgcgt
    23581 ccaacacgtc cacgaagttg gtgcagctgt gccaatccaa atcgatccgc atccatgatc
    23641 agcatgacgg tcgcgttcgg cacgtttacc cctacttcaa ttacagtagt cgaaactaat
    23701 atctgcatct ggttgtcttt gaaggcttcc atgattactt ctttttcctg attcttcatt
    23761 ttaccatgta acaagccaac ttcatatttt ggtgcaaata gttcacgaag tttttcatag
    23821 atttccactg cattttttac atctaatgct tctgattctt caatcagcgg acagatgaca
    23881 tacatttgat gtcctcttgc cagttctttt ttcgtccaat ccagtacact gtccaattga
    23941 ggtgttctta cccagcgtgt ttcgatcggg atacgtccag ctggaagttc atcaatgata
    24001 gatacatcca tctcaccata tgctgtaata gctaatgttc taggaatcgg agtcgctgtc
    24061 ataaataaga catctggtcg ccagcctttt tcacgaagta ctttccgctg gttcacgcca
    24121 aaacggtgct gctcatccgt aataactaga cctaaattct gaaattcgac tccatcttgg
    24181 atcaatgcat gtgtgccgat cacgacgtta atttcccctt gctctagttt ttcaaggagc
    24241 tcacgtcgtt cttttgcttt cgttgacccc gtaatcaatg cgatggttac ttcattggga
    24301 tcaaacagct gttgcaagct ttccatatgt tgttgtgcca agatttctgt aggcaccatc
    24361 aacgcacctt ggaaacctgc tgtaacggta gcgtataacg caatcgcggc aaccactgtt
    24421 tttccgctac ctacatctcc ctgcaaaagt cgctgcatat gctgcggact gcgaagatcc
    24481 cgacagattt cattcgttac acgtttttgt gcatttgtca gttcaaaagg tagcttttga
    24541 gtaaattgtt tcaatttttg gacatcataa aggatctcca aaccattcgt ttctgctttt
    24601 tctgcttttt tcaatccttg aagacgcatt tgaaataaaa agaactcttc aaaaacgaca
    24661 cgtcgtttgg cttgatggtt ttcttcgtgg tcttttggaa aatgcatact tcttacagca
    24721 gaagctcggt caagcagtcg atatttctcc acaaggttgt ttggcagatt ttcttcaatc
    24781 aatgtgccgt attcttgaaa cccttttttg attagatcaa ctaatgtcgt ttgacgaatt
    24841 gatttgttga cgtgatatat cggcgaaaag tcatccattg tttgagaacc taaaatcttc
    24901 atcccgttca aggctttacg ctttgcatcc catttaccgt atacggcgat ttcctccgat
    24961 aaaatgactt tttcttttag ataaggttga ttaaaaaaag aaacgttgaa cacatcatgt
    25021 tcttgcatca tacgaaattg caatcggctt tttttataac caaatcgatt catcacagga
    25081 ggcgagacta cgattccttt caaagtaacc ttttcttgat cctggatctc gtttagattt
    25141 ctttcctgaa tatcttcgta gcgaaaagga taataagtca gtaagtcttc aatcgtttgg
    25201 atgcccaagc tggcaagact agctgctctt ttttctccga ctcctgccag catagtgatt
    25261 ggatcatcta aagaacgcat aaatttttcc tccctccttt aacaaaaaaa ggacttggtc
    25321 agcgatcata cctgatcact aaccgaaggc cttttatgaa tgaatactgt gttagaaaga
    25381 tattctgaca cagaaattat tcagcagaaa atagataagg atagacaggc tgatctcctt
    25441 ggtggatctc gatttccaaa tcaggatgtt tttctgttag tttctcagca aattcatttg
    25501 cttgttcaac tgttccttca tcacctgcca agatcgttac gatctctgtg tcctcattga
    25561 tcatttgttc tagtgtactt aacgctgttg ctgtgatatc tgggttagag atcaggattt
    25621 ttccatcgat catgcccaaa aagtctcctt ctttgatctc tacaccgtca attgaggtat
    25681 ctctgatcgc gtgagtgact tgaccgctca caacgctaga caacatttct gtcatcgttt
    25741 ctttgttttc ttctaaagaa gcttgatcat tgaatgaaag catagctgtc aatccttggg
    25801 aaatcgttct tgaaggaaca actgctactg ggatatctgc tacttctgct gcttgatcag
    25861 ctgccatgaa gatgttctta ttgtttggca agacaatcac tttatctgcg tttacttctt
    25921 gaatcgcttt aacaatgtct tccgtacttg gattcattgt ttgtccacca ctgataacat
    25981 aattagcgcc taaactttca aacagctctt ggacgccttt accagcagcg atcgctacga
    26041 taccatacgg gatacgtggt atttcttttt tcgcaaactc gttcacttct tcatcgtgtt
    26101 ccaaaatcgt ctcatgctgc aagcgcatgt tgtcgacttt gattttgact aatgaaccga
    26161 atttttgtcc ataattcaag acttctcctg ggttttctgt atgcacgtgg actttgatga
    26221 tctcgtcatc attgaccaca agtaaagaat ctcctatacc atctaaatag ttacggaatt
    26281 cttcgtaatc gaatgtacta tcgactgtag gtccttctcc gatttttacc atgatttctg
    26341 tacagtaacc aaaatggata tcttctgtcg ccatttgacc ttggacactg cgatgatgtt
    26401 ctgcattgac catatcatcc atttctgctg gagaaggttc atatgtttca tccgcttgga
    26461 attctccatt caatgcactt aaaaagccct cataaacaaa cagcagtcct tgccctccac
    26521 tgtctacgac accgacttct tttaggactg gaagcaagtc tggagtttta gctaaagcac
    26581 gctttgctcc ttttacaaca gctgtcatta cttcaacgac atcatcagta ctttttgctt
    26641 tcttttctcc agctttcgct gcttctctag ctactgtcaa aatcgttcct tctactggtt
    26701 tcattactgc tttatacgca gtttcaactc catgagtgaa ggctgcagca agatcagaag
    26761 catttaatga gacaacatct ggaatttgct tagaaaatcc acgaaacaat tgagacaaaa
    26821 taacgccgga atttccgcgc gcacccatca gcaatccttt tgaaagcatt gctgctaatt
    26881 ctcctacttt ttctgaggtt gaatctacta ctgccttagc tccactagtc atagatagat
    26941 tcatatttgt tcccgtatcg ccatctggca cagggaatac atttaatgaa ttgacgtatt
    27001 ctgcatttac ttgcagacga ttcgcacctg cctggaccat ttcctggaac tggcttgcac
    27061 ttatttctgt tacactcacc tagataatcc tccttcagat cgactgctga gttaccagcc
    27121 agtctcctgc ctagtctggc agtacacgaa caccttgtac aaagacatta actgaattgg
    27181 cagttacgcc taataatgcc tctagattgt atttcacttt ttcttggacg ttgcgggaaa
    27241 cttcagagat tttcgtacca tagctgataa tcgtataaac atctacagca ataccattat
    27301 cttcttggcg gacaactact cctcttgagt aattttcttt acgcaaaatc tcagtaatat
    27361 tatctttaat ttgatttttg ctggccattc ctacaacacc gaacacatct gtcgcggcgc
    27421 caccaacaac tgttgcaatt acatcattgg taatttcaat ggttccagct gacgtttgaa
    27481 tttttacagc catgaatgag cctccttaac gggtaaaaaa ctcccatttt tcttctatca
    27541 tattttatca tagcgcaact tgaaataaaa ggcagttacc caaagtactc aaaaaccttg
    27601 caaaatttca ggtataacct tgtttaattt acaattattc ttctcaattg tcaattgtat
    27661 ctatgaaaat gtacttgcaa attcccattc tatatgatat gttagtctgg tatgagctga
    27721 attattctat tgctccaaat caaggcaaag gaggaaatta ccatggcaaa agtttgttac
    27781 tttacaggtc gcaagacaaa aagtggaaac aaccgttcac acgctatgaa ctcaacaaaa
    27841 cgtactgtta aacctaactt acaaaaagtt cgcgttatgg tcgacggaaa acctaaaaaa
    27901 gtttgggtgt caactcgtgc tttgaaatca ggaaaagttg aacgcgttta attgaatgat
    27961 acttggaccg ttcagatagt gaacggtttt ttatttaata aaaattttta agggtgggac
    28021 ttttgattcc tacccttttt ttgcgtttat ttttgttata cgaac
//
LOCUS       DARBEC010000028        27964 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.028, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000028 DARBEC010000000
VERSION     DARBEC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27964)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27964)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27964
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.028"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(424..1368)
                     /locus_tag="SCL80_001162"
     CDS             complement(424..1368)
                     /locus_tag="SCL80_001162"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEG1107044.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(1664..2608)
                     /locus_tag="SCL80_001163"
     CDS             complement(1664..2608)
                     /locus_tag="SCL80_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107045.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            2829..4043
                     /locus_tag="SCL80_001164"
     CDS             2829..4043
                     /locus_tag="SCL80_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107046.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            4065..6080
                     /locus_tag="SCL80_001165"
     CDS             4065..6080
                     /locus_tag="SCL80_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEG1107047.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(6163..7536)
                     /locus_tag="SCL80_001166"
     CDS             complement(6163..7536)
                     /locus_tag="SCL80_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1107048.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(7562..7846)
                     /locus_tag="SCL80_001167"
     CDS             complement(7562..7846)
                     /locus_tag="SCL80_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1107049.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(7848..9863)
                     /locus_tag="SCL80_001168"
     CDS             complement(7848..9863)
                     /locus_tag="SCL80_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEG1107050.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(10042..10347)
                     /locus_tag="SCL80_001169"
     CDS             complement(10042..10347)
                     /locus_tag="SCL80_001169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEG1107051.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(10436..11467)
                     /locus_tag="SCL80_001170"
     CDS             complement(10436..11467)
                     /locus_tag="SCL80_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1107052.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            11674..12549
                     /locus_tag="SCL80_001171"
     CDS             11674..12549
                     /locus_tag="SCL80_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1107053.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(12701..13543)
                     /locus_tag="SCL80_001172"
     CDS             complement(12701..13543)
                     /locus_tag="SCL80_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1107054.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(13635..15512)
                     /locus_tag="SCL80_001173"
     CDS             complement(13635..15512)
                     /locus_tag="SCL80_001173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1107055.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(15509..16930)
                     /locus_tag="SCL80_001174"
     CDS             complement(15509..16930)
                     /locus_tag="SCL80_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1107056.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(16933..20322)
                     /locus_tag="SCL80_001175"
     CDS             complement(16933..20322)
                     /locus_tag="SCL80_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEG1107057.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            20579..21970
                     /locus_tag="SCL80_001176"
     CDS             20579..21970
                     /locus_tag="SCL80_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107058.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(22043..22690)
                     /locus_tag="SCL80_001177"
     CDS             complement(22043..22690)
                     /locus_tag="SCL80_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEG1107059.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(22698..23384)
                     /locus_tag="SCL80_001178"
     CDS             complement(22698..23384)
                     /locus_tag="SCL80_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107060.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(23390..24463)
                     /locus_tag="SCL80_001179"
     CDS             complement(23390..24463)
                     /locus_tag="SCL80_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107061.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            24685..25359
                     /locus_tag="SCL80_001180"
     CDS             24685..25359
                     /locus_tag="SCL80_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107062.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            25364..26704
                     /locus_tag="SCL80_001181"
     CDS             25364..26704
                     /locus_tag="SCL80_001181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1107063.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(26853..27839)
                     /locus_tag="SCL80_001182"
     CDS             complement(26853..27839)
                     /locus_tag="SCL80_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEG1107064.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
BASE COUNT         8164 a         5273 c         5063 g         9464 t
ORIGIN      
        1 aaaaatgata aacctctctc gaacggtttt ttatcaaaaa tcgtcatttt tccatcaaaa
       61 tctgcttatg caaaattata aaaaaatttt gcagtttttc ttactttttt tgtcgtttaa
      121 ttgaatgata aaaaccagtt tctttattca gttaatcaaa tatgaacgaa ttagatatga
      181 atcattgtat ttgccgttgc ggaaggattt tttaattaac cacagataaa cattgataac
      241 gattcgctta ttgtaatcaa cagattaaat ttcatatgtt catatgtacg gatacatttt
      301 cggaccgatt aattttaacc attctgttta tttgcttacc tattagatat caataaaaaa
      361 ctgcttaaca gctatttcag ctgttaagca gttttaggat ttttagttca tattcattgt
      421 ttcttataat acagtgttca atacttctct taaagcactt gttgatttgc tgaacattgc
      481 tttttcttct tcgttgattg ataattcaac aacttcacga acaccatttt cgtcaacgat
      541 tgatggcaca cctgtaaaga tatctttttc gtcgtattca ccagttaaat aagcagatac
      601 aggcaataca gcttgttcgt tattcaagat agcttttacg atacgtgcag tactcatacc
      661 aataccatag taagttgctt ttttacgatc aatgatctca taagcagcat tacggacttt
      721 atcagcaatc acatctaatt catcttgggc aatacgatgg tctttttcta caatttcaaa
      781 cactggttta ccaccgacag ttgtatgtga ccatgcagca acttctgaat caccatgttc
      841 acctaagata tagccgtgga cgctgcgtgg gtcaactttt aatttcaatg cgatttcttt
      901 acggaaacga gttgtatcca atgttgttcc agtaccgata acacgtgatg ttggcagacc
      961 agattcgttc caagcgatat atgtcaaaat atctactggg tttgaagcaa caacgataat
     1021 accgtcgaat cctgatccca tgatttcttt tacgatttgg cgcatgattg acgcatttgt
     1081 tttaactaaa tctaaacgag attgaccagg tttttggttg ataccagctg tgataacaac
     1141 gatatcagca tctttacatt cttcatagcc acctgaccat acagccacat tttcatcgcc
     1201 ccaagccata ccatctaaaa ggtccaatgc ttccccttct gctttttctt gattcacatc
     1261 gatcaatact aattcattag agattccttg gttgatcatt gcataagcga tacttgttcc
     1321 aacaaaacct gtaccgacga ttactacttt acgacttgtt tttttcatga ataaacagct
     1381 ccttttcata actatttgtt actttcatga aaagtatagt cttgtttatt cattttatca
     1441 acatgttaat tataatagtt gttattataa tttttgatgg aaacgaattc ctgaatacgc
     1501 ataacagata aatgacaaat gtttacacct atatcccctg ataatacaac caaaagttta
     1561 gaactgaaaa taaatccaaa aaaattcgct gcaacgatat aataagacag caatcgcacc
     1621 taaaacaatt ttccttcata tgctttagtg aaagattttc ttcttaatgt ttatttctcc
     1681 attcattggg agtacatgtt gaatacttct tgaataattt gaataaagaa gtcgtttgtt
     1741 tatatccgat acgattagcg atttcttcca acgaaaaact cgtttcttga atcagttcca
     1801 acgcaaagtc gaattttttc tttagaacca gttcttgaaa ggaagcatca aaatacattt
     1861 tcaatttatt actaagatag accggattgt agccaaaatg agctgctgtt tcttttaacg
     1921 tacaagaagt aaaatgctta tcgatataat ctaatactct aagcatctca ttttctgcgc
     1981 atccatctgc tgatgaaaag ctttgttgtt ctaataaatg agtcaattcg atcagtaata
     2041 gtgatgtata gagatttaac gaacggatct tatgactgta tgtatcaaaa taaatcaaca
     2101 gcatattttc aatcaaatgc gtcaaacgac ttgtttttcc gccgcgaaaa tgaatgaatt
     2161 gtgtatgttc ccctttttct tttgatgcat ttatcaaaaa agaagaaagt agattatttg
     2221 tgcttgccaa atgattcaat atctctgtag ataaggtttc ttcttttaat aagatgttga
     2281 ttaaaatatc ctcttgccct gtgtattcga ttctttgagt agcttcttta tcaatgatca
     2341 ccacgtcact ttcttccatc tctatccttt ctccattaat aatttgtaaa catgtaccgc
     2401 tatacatata attgatttca acaaaatcat gtgtatgctc gggaacttcc gcatatcgac
     2461 ggtgtttact gatatgaata tttccctgtt gaaatagatc ccctcggtgg atttttggcg
     2521 gtgtctgaag atggtaagct aagtttaaat cattgatatt cattccttgt tcacgctgca
     2581 gagtttcgat tgattcttcg ttacgcaaaa aaaatgtcta attcagctag ttccaattga
     2641 ctccctcctt tttgtaagcg gtttctttcg cttattagtt cacgctgtac tgctattata
     2701 tgcaaagcta tttttggttt cataaagact ttctatggtt gggacatgaa cttcctgtga
     2761 ttccgctatt gagagaataa ccacttcata ctatattttt gataagcgct tacaaaaatg
     2821 aaaggaacat gcttatgaat aaaaagatga tgaaattttc tctgctgtca gtctctctta
     2881 ttttgacttc tgctggtgca gtcacgtctg caattcccag tatgtcagct acttatgcca
     2941 cgcgttcttt atctgctgtt gaattattaa cgactgctcc ctcttttatg gtcactattt
     3001 ttgttttgat tagtagtttt attgctagga aaatcggaca aaaacaaact gtgatcatcg
     3061 gattagtgat tgcatcgatt tgtggtatcc tccccctctt ctcacaaagt tacagtattg
     3121 tcttagcttc aagagctgga ctaggtattg gctttggatt gatcaattct ttggcagtca
     3181 gcatgattgc tgccttttat cacggagatg aacgagcctc tatgattggt tttcaaagtg
     3241 catttcaagg atttggtgca gctttaatga catttgttgc aggacagcta gtacagttcg
     3301 gttggcaata taccttttta gtctacgcca tcagcttacc tattcttgtc ttgtttgttt
     3361 tgtttgttcc tacacctgaa acaactgaac ttcaatcgca tggcacagcc ccaacaagac
     3421 aagtaatcaa aacgaatcct aaaatgatta gttatggtat ttttctcttg ctggtcatcg
     3481 tggtatataa tgctgtatcg attaaattag ccacagtcct gacgacttat tctctaggaa
     3541 acgaaactaa tgcagcgaat atcttaacaa tcatgcaatt ggcaagtatg gctgccggag
     3601 tttgttttgg ctggcttcag acaaaagcac atcgctatat gcttacactt tctttactac
     3661 tgatgggaat cggctttgta ttgattgcca ctgtgataaa cctgtatgtg attgggtttg
     3721 gtgcgatcct aacaggaatt tctttttcgt tattcattcc ttacttattc aaccaagtaa
     3781 gtatccaagc gcccaagtac gcggagagtt tcaatacttc aatcttattg gttggcgcta
     3841 atttaggtac tttcattgca ccatatggtt tgctctttct ctcttttgct tctcttggga
     3901 gtaaaacact tgctgttttc gtcaatggca gtatcttgct gttgattagc gcgcttatta
     3961 gccttctagt agtacacagg acgaatagaa agacagtgat aagagtagat gaagaaataa
     4021 aagaacaatt gacaacaaaa taaatttgat ggaggtcgtg taaaatgaca aatgaaaaac
     4081 ataaagatag atggctttgg tatcctggag actttgagat ccgccatgga ctgctacaaa
     4141 atttccaacg ggaagaacga ggttttgact ggccagcgta ctggtacatg gatgattgcc
     4201 atcggaacgt caagtttaag agatattact ttcttgatca accttccatg tttaaagtga
     4261 cgatccaagg agttgggtat gtagaaatta atggacaaaa acatccatgt ggaaaatggc
     4321 tgacttgtcc agctggtaaa gcaaaaattc gtatctttgt aggacataca tcagggctac
     4381 cagcgatgtt tatcgaggga gacgaagtaa aatcagatat tggctggaca gcaagtaatt
     4441 ttattgaaga atatccagct ggctggagtc cgctttatgt cgatatcgca aaagatccaa
     4501 atcaaatcta ttatcaaaaa gaatgcgtcc atccagtcac tgaaacggag attggcggag
     4561 gaatcttatt cgattttggg cgagcagtga atggctgtgt agaactaata tctctggatg
     4621 accatatcga atctatcact ctatgttatg gagagtccga tgtagaagct atggatatcg
     4681 attattgcta ctataagcaa ataggaatga caagaaaaat gaaagctcgt aaacgcgctt
     4741 ttcgctattt gtttatacca gaggtttcta ctggaatggt tcaacttcaa gtttttcatg
     4801 aatttttatc tctttcgaaa caagctgctt tttcaagcga ttcttcttta ctgaatcaga
     4861 tctggtcagt atcagaagaa gccttcaagt tatgtagtga tttgtttttt atcgacggta
     4921 tcaaacgaga tcgctggatt tggtctggag atgcgtatca atcattcatg atcaatcaat
     4981 atcttttttt tgacgaggaa atcaataaac gaacgatatt agcattaaga ggacaaaatg
     5041 aaatcaagca gcatatgaat actatcgttg actattcgat cttgtggttg attggtatcg
     5101 aaaaccacta tatgatgacg aaagactatg aatttttacg catcgtctac cctaagatgc
     5161 aatcattaat gagatacttg atggagcaaa cgaatgaatt agggtttatt tatgggagag
     5221 caaaagattg gatatttatt gattggtctg aaatggataa agaaggaacg cttgctgcag
     5281 aacaagtctt attactgaaa gcctatcatt ccatgatcac ttgcggagat atcttagctg
     5341 aaacatatgg agagaacgaa aattatcata ctcatctcta tcaagaaaaa tatcaactat
     5401 tgaaacaaaa cctatttgac tacttttggg atgaagaact tggcgctttt atcgattgct
     5461 atgaatctgg gagaagaaat gtgacgcgtc atgcgaatat tttcgctgtt ttattcgatt
     5521 ttgctaacaa agaccaacag caatcgatct taaagaatgt cttattgaat gacactatca
     5581 cacagattac tactccatat ttcaaattct atgaacaaga tgctttatgc aaactcggac
     5641 agacgaatat cgtctaccaa acgattcttg attattgggg agggatgtta tctcatgatg
     5701 cggtaacttt ctgggaagaa tacgacccta ctcaaacagg aaatgaaaag tacagtatgt
     5761 atggagatcc ttatggaaaa agcttatgcc atgcatgggg agcaagccca atctatcttt
     5821 taggacgcta ttttatcggt ttgtatccaa caaagccagg ttatgaagaa tttatgatac
     5881 aacctaatct agattcattt gaaacactga attgtacgct acctatcaaa gatggaaaga
     5941 tttcgcttga aaaaacaaaa gataagatcg tgattacttc tgatagaaca ggcggaacag
     6001 tgaaaatgaa tggtaaaagt tatcctcttc tagcaaaaca gccacttgtc ttaacaagct
     6061 attcaaaaat aaatgcataa aacaaaaaac tgcttgcgac atatcagaag actttaaatc
     6121 ttcttctgat attgtccaag caatttttat tttgaagttt gcttaattct gttctttaga
     6181 aacaatgttt gttctttttc cggcaaaaag aaagctagat aatacgacaa gtaaaaagaa
     6241 agcgatgatt ccaactgtca aggctatttg accgtattct cctagtttcc caagtacaag
     6301 tcctggtact agataatcag tatcggcaaa tgtagaagaa gcgattttaa gatctcctaa
     6361 tactggaagg ataaataatg gcagccaact aatcaataag ctattgaaag cagaacctaa
     6421 taccgcccct cttctaccgc cagtcgagtt gccgaagaca ccagaagcag ctccgcagaa
     6481 gaaatgtcct actaccccag gaatgataac tgtagtttgc aagcctatca tagcaaacat
     6541 ggatagtgtt cctacaaaga agctaacaaa aaagccaatt aatacagcat taggtgcata
     6601 tggaaaaacg ataggaacat ctaatgctgg tttagaatcg ggtacgagtt ttttagcaat
     6661 cccttggaat gctggaacga tttctgccaa caccattcga accccttgaa ggacgataac
     6721 aaacccagct gtgaatgttc cagcttggat cagtgcaaag attacagcgt tgtctccatt
     6781 gcttagttct ctttcaacaa atccaggacc agctaacaaa gcaagtgcga catatacgat
     6841 cgacatcaag agcatgatcg atacagtaga atctcgcaaa aagctgaaac ttttaggaat
     6901 ctttatgtcc tctgtagaac ggtctttgtt tccaaaaaac tttccgatcc atccactaga
     6961 agcataaccg atatttccgg tatgccctaa cgcaacttga ttgtttccag tgatgttctt
     7021 cataaacggt tgacaaagag caggcgtaat agttaatagt gttccttcga agaccccgcc
     7081 taataagatc atccacaaat ttgcagtaaa ccccgcgctt actaaaatga ctgctgttag
     7141 acaagaaaca taaagcattg cttgacctgt aagaaaaatg aatttatatc ttgtgaatct
     7201 agcaagtaaa atattcacga tcatccctac tagcatgatc aaagcagtag gtgtaccgta
     7261 ttgttgcaaa gcaatcgcca ctactgcttc attgctagga actactcctt gagtatgcag
     7321 agctgtttgg aacatcgaag caaaaggggt tagtgaatta ctgattaaac cagcaccgcc
     7381 gatcagtact aggaatccga caaatgtttt gatagttcct tggatcactt ctgaaaattt
     7441 cttcttctga agaacgagac caagcagtgc aatcaaagaa acgaggatgg agggctgact
     7501 gacgattgac acaaacaaat tcaacaattt atccattgat agttcctccc ttgaatcatt
     7561 tttattcttt tgacaagatc ttcgttactt tttcttttac ttcttcgctg tcaatgatcg
     7621 attgcaaagg tacgagcttt tcatcaggta tagaagttag cgtatctttc aaagaatgct
     7681 caatgaagaa ataatcagcc atatcgctag atacacttcc aacatccgta tgacttactt
     7741 caaactcgtc cacatctgct ccaagatctg caagtgcttg tttgatgttc atctctacca
     7801 taaaacttgt tcctaatcca ctttgacata ctgcaaaaat tttcatatca ttcgtctcct
     7861 tttcttatga tcgctaatat ttcttctttt gttgatgcgt gtaataaatc ttgaagattt
     7921 tgtttattag aaagtagctt tgttaaattt gccaaagccc gaagatgtgc ctcattatcg
     7981 atagcagcta aacagatgaa caaactaatg ggatgggctg gattatcaag cagatccaca
     8041 ggttgttgaa gtttcagcaa agacatccct acttcgttta cgccatcttc tggacgagca
     8101 tgaggtaatg cgatgtgatc gccaatatgg ataaacgcac catactgctc cacgcgctcg
     8161 atcatagctt tgatataact ttcttctatt ttattttctg ataatagcgg ctgtgcggca
     8221 aatgtgattg cttcttgcca gttttttggt tgatccgata attggatcat ttcttctgtt
     8281 aataattcag atagcatagg acgttgatcc tccttctttt ctaaaagttt gttcatttta
     8341 cgatttaata cttgatagag cttttcttcg tctacgcctt cttttaattc gatataaggc
     8401 agtagcgccg acaacaattc ttgggcagaa ggcattgaaa atcctggtat cagccaatct
     8461 ctttgtactt gattgatcag ctgatttttt tccaagtcag tcatgattgg atgcagaaca
     8521 tagagagact ttttcttatc agtaagtgtg agaggtacag tagaaaaaat aatatcgtaa
     8581 gaagtttcgt caacctcgtg taagcggtca gctgaattag tctctgtaaa aaagatcatc
     8641 gggaataatt gctgaagttc actttttaag atcaatgaag agctaatgcc acttggacag
     8701 acgatcacgg ctcttatttt tctatttctt tcttctgcgt ccgcttttct gatctctccg
     8761 ccaaatagta tagtgaaaaa accaatctct tcttcaggga tgctcttccc tattcgtctt
     8821 tctaacggaa gtaatgcttt tcttgtcatt tcgtagatgg aagcatattg atgcttaatt
     8881 tgatcgatca tcacattagg taaataaaaa gaataggaaa tacgaaaata ggcagggacc
     8941 aagtgataaa agacattcat caataattca cgaaacgaag caaattctac ggccatcagg
     9001 aattccatgc gatggatgac ttctgaagaa cattccagca aaaaatcaag tgcaggctcc
     9061 tggacatctc cttcaacgat cgatgctaag caaagagaga aaaatataat ctccgaattt
     9121 tgatgttcgg aatagccaag gccagtcaat acttgtcgac taatgtcagt gactgcaaga
     9181 gaagccaaca gttcagcatc ttttaccagt tccatggatg tttttccaat tcgaatggtt
     9241 gtcaaactca aataataagc taattcatcc attcgatttg gtgcgaatcg aagatctgat
     9301 agtgaaatcg ttttatctag caaatctcta actttggcgt aataatcaat ggaaagggtt
     9361 gcggttaaat acgtgagccc aaatcttcca gcaagtgact gcatcaaacg agcagtgcga
     9421 ttttttgctt ctcttcttaa tattgcttct gaccctagca gcctaaaacc agttttccta
     9481 gtatacgaca gctgacaatc ttccacttct aattcatttc ttatcttttt gatatctgac
     9541 aggactgtcc ctttagaaac ctgaagaaaa tcttgaaaat gaaaaacaga aacagacgtt
     9601 gcagtcgaat aaaccatcaa ataaagaaga tctcttcgct cttgttcggt atacacagct
     9661 tcaaatcgag taatatccaa aaatttttct gcccagtcga ttgctcggaa tttctttata
     9721 cggatacggg tatcatccac atattcggca aaaggagtta attgtttgat cgcctcgctt
     9781 gtatttactt cgatgatctc tgctgctttt tctagactga tttctcttgg tgttttcagg
     9841 agataactaa ttttgtagtt cacagcgctg ttcacctcct acttatattc tagtatggta
     9901 gcgcttttaa atcgaggggt gtttggtaca acttacagtt ggattttttt tacgactgta
     9961 ccgaacgatt tctatcgaat ggatacagaa aaaaagcagg tgaccaaatg tctcctgctt
    10021 ttatgaattg atttcgtccg atcaatgatg atcatgttta ttttttgagg tcagttcttc
    10081 aaatttttct ttgacatctt caaccgcttt ttcgatatct ttttcaaagt gattgggctc
    10141 attaggcttt gtttcctctt ctacagaatt tgaaccagat aaagctgttt ctttattttc
    10201 tgtttcacca gagtttgtgc cagctaatga ttcttctttg tcttctgttt caccagaatt
    10261 agttcctgct aaagccactt cttcttctgt tttaggggcc attgaacctc ttaatcgttt
    10321 gtgtttttca ccgtcataat ttgtcatgtt ggttcctcct agtagttcta tactttttca
    10381 tcgtacaata agacagttta tgcgtcaaaa aacaaacccg attcttacaa atatgtcata
    10441 tttttccttc tgattcttga atgcttttga ttccttcttc caaaattttc atttgtaaga
    10501 ttgtttcttc atctttgatg actttttctg cgccattaac taatgtctcg taaatccctt
    10561 cgtagacacg gctgtaatca ccaacctcgg aaattacttt ttcttcatga taactccctt
    10621 gttcatctat ataggtcaat acaccataat gttcgggcaa atcgatacca aagtcttcat
    10681 gatcaggcat gtaaaacatt ttcagatgtt cttcttgtcg atcttttgtt tgtttcacga
    10741 acatcccttt ttttccgtaa acgacgaaac ttggacgttc tttgatgcgg aaataactgg
    10801 attttatcga aattttcata tttccataga aaaaatccag atcgaagtaa tcattcattc
    10861 gtcctttccc taaaagttga cgtacatcat agctgatatc atctggttcg ccgaaataag
    10921 aaagaacttg atctactgta tgacaagcat gtccatataa atagctgttc gcagtagaaa
    10981 attcatgcgt attttctggt acttccggtc tgaaataatc ataatgcatt tctacttcta
    11041 aaatatcccc taattttccg ctttcgatta ctttttggac tgtcaagaag tcagaatcga
    11101 accgtctgtt ttggtaacat tggataaata gacctttttc ttttgctaat gcaaataact
    11161 cttctgcttc ctttgacgtt tctgcaaaag gtttctctac tagtacattc ttaccatgtt
    11221 cgagtgccat ttttccatat tcataatgaa attgactagg tgtagtgatg acaactaatt
    11281 ggatctcagg atcatcccaa agatcagaca gctgatcagt atattcgatg tcttcgaatg
    11341 ttttccacac tggttcagat ggggaaaaaa tttttttgat attgattttg ttcgtaagtt
    11401 ttcttgaaaa aggcaaatga tagcgatttg tactctttcc attgccaaga taagcgattg
    11461 taagcatgag tggtgcctcc ttttattgat gttttcagta tatcctagaa aagcgcagaa
    11521 agtatttata atagctattc tggtaaaaaa gttcagaaaa acacttgtta tcattcggtt
    11581 tggtgaaatt aatgttcaaa gtaaggataa aaatcaaaaa gatagtagcg gaaccaagtg
    11641 tttatcagta agatagaatt tggaggtcag atcatgctta ttgaagaaaa actaacaaaa
    11701 caagagttgt ttaccacgac agagaaacgg attgtcgatt acatccgtag gaatatcgaa
    11761 gctgcagtct acatgacgat tgaagaatta gcaaaagcta cgtatacttc ccattcagct
    11821 atcattcgtt tatgcaaaaa aatgggattc agcggattta aagaattccg tttcgagctg
    11881 gggcgggaag ttcatcaatt gattgctcag ttcaatcaaa cgattgatcc taattttcct
    11941 ttttcaagtg aagatagcac tcagcaaatt gcaaagaaaa tggcggattt atcgatccag
    12001 tcaataaaaa aagcacaatt gcagattgaa catgaagatt tagatcaaat cggtaaagta
    12061 ttgaacaagg cacagcgaat ctttcttttt gcaaaaggtg attctcaaat cacggcaagg
    12121 aaattccaga ataaaatggt caagttgaat aagtttttga tcatggctga agaatacagt
    12181 gattcttctt ggaatgcagc caatttgact agcgaggatt gcgccatttt tattagctac
    12241 agcggtcgca tccatcatta tgaacgaata atgacctatt tgaagcatgt agaggcccct
    12301 accttgctga ttaccggaaa tcagcattca gaaatggcca aacaggctga tatgtgtctt
    12361 gttatatcgc aggaggaata tgattttgct aaagtagcga ctttctcctc tcaaattgcc
    12421 ttcgattacg tattaaatac gttattctca gtgatatatt ctcaaaattt tgaagagaat
    12481 atccgcaact tgaaaaacaa acaagaattg atgcaaaaag gccttcttaa agaatatcgg
    12541 aaaacttaag aaaagatcct catttagtaa taagaaagat agtgtttgat aggctctctt
    12601 tgatatcatc ataaaaacag gctgaagttt tctttttcaa acctcagcct gtttgtttac
    12661 ggaacgattc gaatagatat attatagaaa tgaggaatag ctaacttctg tttcttattt
    12721 tgaacgattg acgaaaaaga tatataagaa ggaggaaaat cagtatacat gcagctaaca
    12781 atccatacag gaaaaatcgt ttgttctcgg atactttctg tacttctttt tgaacttctt
    12841 ttgcatgata aggaacccgg tgccctctga caagtaagcg atgactgttg accatataag
    12901 gtgtacaagt taggagtgtc acgaaatcct gccctttttc gattctcaga tcttttgtat
    12961 cagtcggttc taccgtcttg atctgatcga cttgataagc gagtgtccgt ccattgattt
    13021 cgatatagaa agaatcacct aaagtcagtt tcggtaagtc tgtgaacaac tttgcttgag
    13081 gcagtcctcg atggctggaa ataacggcat gtgtattttc tccccccgta ggataagacg
    13141 ttccttccaa taatgaggca cctttttcta gcagcacagt attcgtttca tcaaaaattg
    13201 gcaaatggac attgatctca gggatcgtca aaataccgat cgtatgtttc tcaaaatagg
    13261 ttctatcctt tttcgcaggt tcttttttct tagtaaaggg atctgcacct ggatttccac
    13321 cttcttttgc taattgctgg tttttctttg tcatgttttc ttggatttta gccatgactt
    13381 cttcattttc tttgaccgct tgttgctgat aatgagagat gatctgctga tccagataat
    13441 tattcagcgc gtcgctaaca aatggataag ctagtatccc tattcctgag agaagcaaga
    13501 aaagcatcag cagatctaga agtttccttt tctttggttt catttgtgtt ctccttgtga
    13561 tgactgtttg ataataaaag aaaaaggagc atttgcgtaa tccgctgctc ctttttccaa
    13621 ttcttgcaaa aagactaaat ctgactgtgc ttgcgtctag caaagtacag tccagcaatc
    13681 agtagaagga ctactcctgc accgatatac acatagattc ccttaccgcc tgttgaagga
    13741 agtgtacctt tgtgtttatt tggtattttt tcaggagaaa tcaactgtcc tgctgttaca
    13801 taagattgtt catcgatagt aaatgctaca cggtttgtta atggaacata tttttctggc
    13861 gctttcgttt cttccagata gtacgtgcca tattttagac ctgtcacatc gattaaaccg
    13921 ttacttgtgg ttgtaaaaac cgttgctgat tctttcgccg atacccagct gacggctttt
    13981 gtggatgggt cgatcgataa atatttcgct gtgtcactat cttgatcacg aacgacgaat
    14041 tctgctccag caagtgtttg gtctgatgta acgtcaccat ctactttaac aaatcgtttg
    14101 cccccagtaa cgacatcgac tgacggtggt gtttgatcat ttgtatgccc gttatcgaca
    14161 tttgcttggt tagaaaatcc tttggttgga tctgcttttt cgttcaaatg catatagtaa
    14221 acgaatttca atgtaccgcc aggagttaat gaaggaatat aattcggatc aactgaaacc
    14281 gtgaatccgt ctgtttgctc agtgacagaa taatttactg ggtcgatttc tttatttcca
    14341 tcatataagg cataagcata cgttcctgaa gaatcattat caaatgttaa agcagcgtca
    14401 tgagtatcga taagtttgaa tgttgtgtac ttgttttgcg ttccttcttt atcagcgata
    14461 cctaatggga tattgacaga aatctcatat tgaatttttt caccgattgc gacatctttt
    14521 ccattcaatt gaggtgttgt tttatcaact ttagacgtat catttttgat ggtcttttct
    14581 actggtgttt ggctatttgc caggatctca aaaggcgtga ttgtttgctt ttcaatcatt
    14641 tccgcattat caggagcttt tacttcttct aaattatatt ttccaacttc taaatgatta
    14701 acgatcaact ggcctttttc aatagatgcc tcggcaaagt cattgttgcc gatgtcataa
    14761 gaatgaccag taatgaaatg tttggctttg gtttgatcag ttgtccaagt gtacaatcca
    14821 tctgtgacac tttggatgta ttttttgacg cttggtgttc cttcttcttt agaaataata
    14881 aattctgctc catttagtgc ttcgttttcg gcagtaccga tttttgtaac tttcaacgtt
    14941 ccatcattac cgactgtatt tttagggtag agatggatag tatctagttc ttctgtcccg
    15001 tatttataag agccatctgc ttgtttgatc atctcatata cagggaaagc taaaaccatg
    15061 tttgcggcag ctgacactcc gtcttttggt tcttctttga tcgtgtagac tgcatctttc
    15121 ccattttgtt ttttaggtaa agataaagtg acatttccat cagcatctgt cgttcctgaa
    15181 gcaactggtg taccaggagt caaagactgg actgcttgtt ttgctgcatc cacggacgct
    15241 cctttatctc gttgcgcata aaattcttga gtgacgttat aaactgaaaa tgaaatatcg
    15301 gctaatcctt ggtattgatc gaattcgctc atttctttcc cgctgttttg gattaaagga
    15361 tcgggtaaat cagtcatttt ctttttatga aggatgactt gagctgctgt ctcctctgca
    15421 aacacttttt ttgcgccgaa gctgtttgtg agtaatggta aaataaggaa aaggactcct
    15481 aacataacgt ttattttttt atgatttttc attttttcac tcctgttctt tttttgataa
    15541 acaaccagat tgcaaagctt gcgaatacca taccaacaac gcctaaaagt gtatttttcg
    15601 tttcgtttgt ttttggaaga aatggacggc tattttcttt tttattgact actttcaata
    15661 tactgtccgc ctctttatcc tcagaaagaa aagttgaaat ggtaaacgga atttctgttt
    15721 cacttaaaat ataaccagaa ggtgcttcga tctcttttaa tcgataactt cctgttttca
    15781 acccggaaat tgaaaaagcg ccatttttat cggaaatcag gtgaagcgca tctgattcgt
    15841 tctttgtcca cttgtatccg ttggctgttt gctggaggta atttcctgct ttgtttttaa
    15901 taaggaatac tgcgccagct aatcgtttat ctggtttatt tgtctcgatt ttgacaaatt
    15961 gcttactccc tgtatataca gagactttct ttgtactgac accaaaacca aaatccagtg
    16021 ttccgttgtt gttgatttcc ttgttcgcct gtgctgtact gcttagctgc atctgataag
    16081 aaatggtcag tttttttcca gcatattttt ccaacgaagg gatcgaaaat aaaacatcaa
    16141 atccgtgagg tttcttgtgt atgacaaagc cttctgtcag cttttctcca ttcgatgaaa
    16201 tcgttaaact attaggtaaa aacgttaatg cttcatccgc actatctgac aattcgaact
    16261 tttgatagtc aaggatattt acaggaattg tcgtatgcaa ggaataagtg attttttcac
    16321 caatcgtaaa atcatttcta ggctcttcta ataccttctc aaaaggtgga tcagggtagt
    16381 cgttttcttc atttttagga tacaaatgga tagttgaaag tttttgattg ttttgtccat
    16441 gtacaggaag aacaactacc atattctctg ccttttcttt gacgacttct ggtactttgg
    16501 attcaatgaa aagatacgct ttatctcttt tttcttgatc tttagcggcc agagaaaaag
    16561 aagcgatacc atcttcgttg ttaagagttg tagttgttgc ttctgcggta aacataccgg
    16621 aagacgcacc gttttttgcg atctctgctt gtgcttcttc taccgttttg cccttttccc
    16681 gtagatggta aaaagaatct gtgacatcat aaacttggaa tgtgacacca tttaatcctc
    16741 gatacgtttg taataaagct ttttcttgtc cgtcatttgg atgatttttc ggcagttgcc
    16801 cgtttgggaa aagcaatttg tgtaaagtaa tctgaaccat ttctgtttct gtttctgttg
    16861 ctacctgagt aaaagctggt ttaaatagta acaagaagag acacatacta agccaaccaa
    16921 gttttttcat atttagctgc cccctctttc ttttttcaaa aaaagatacc aaatagaaaa
    16981 cgcacaacca atcatcccta ctagatagat tcctaaacgg ccaattcctc ccgtttcagg
    17041 taatggtact tttgcctgat tcgtgatgtc taaagaaatc tggttgtttt tggctcctgg
    17101 tgacagaaca gattcatgat cttgtccatc cacttgaatt gacccatctt cgtgtataac
    17161 tatagtaact ggctctttta gaccttggta tccttctggt gtaaaagttt cggtcagcgt
    17221 ataagttcca ggttttaaat tttcgaatag aaaggtatct gtttcttgtc catcttttgg
    17281 cgattctaag tccatttctg gtccctgcag tctgaatttt gctcctttta atgtctgtcc
    17341 gttgtcttct tttttgtgta ccgttagatc aaacgctttt agtcgatttt gatgagtgag
    17401 cgtccataca ttatttgtca tctcggtatt ttttcccgtg taaatgattt ttccatattt
    17461 atcgatttgg aattcataat ttcccggcga agtatcatat ccggcaggac cagctgtttc
    17521 aacgactcga tatttaccag gtgtgagtga atcaaaactg gcaagacctt tttcattcgt
    17581 tgtttggctg tcaattggct gataagtacc atttgcttct tttttctgta gggaaaatgc
    17641 tgctccttta agatttatct ctttgtccgt cccttgcatc gcatacttac gtactgctac
    17701 aggaacttca acaaaaggat tttcaatagt caactggatc gtatcatcgg aagtagtgac
    17761 tgtttttcca tcaatggtta ccgtaccatc agaagcgatc ttgatttccc aagtagtctt
    17821 tttgcttaat ccatgccctt ctggagcttt tgtttctgtc agcgtatagg tcatttcttt
    17881 ttgcaatttg acattttctg gaagagaata ggttccgtcg ccatgatctg ttagtaaagt
    17941 atcaatagaa tctcctgtta atttgaaaac agcgccaata agatcctttt cacctgtaga
    18001 ggaatttttc gttattttca agtttaacgg tgtgaattgt ttagtatttt tccatgtcat
    18061 tgcatctatt tgttgagaat cgtatcctgg tacaggtaat tctttgattg tttggtaact
    18121 gaatgcttga ccttgattat tgtattgagg tagtgataat atctcttgat aactttctcc
    18181 gctatttgca gataatttgt caatgtctgc acgttcccac gtatttgttg tatctttagc
    18241 tggtttaatg attcgaatat atccgttttt ccaagctgca gcatttgtcg tatgttcccg
    18301 ttgaatctca aaagtaactt gatctggacg atcagctaga ttgttgtcaa attcttccca
    18361 taactttttg atgtgaagac tgactccggg agctttagca gatggtatac cgaattcagc
    18421 taattcattg gtatcaatgt ttggctggaa agttgtcctg ccgttcattt gataccagaa
    18481 atttggatgg aagtcctcat tttctgtttg gattctcact tggtaatgga tttggatttc
    18541 ttggtctttt cctaaataga tctgattgct cttgatggta tttccttcta tggaaataga
    18601 tggagtgacc gttgtaggac ttgtccccac actcttgact gaaagtgtac caggctgata
    18661 aatgaatggt tctgcgattg gatcatttat ttgtccattg cttacagttg ctgagatctg
    18721 tacagctttt ttggctaggt attcagagat atctgttgca tgatcagctg attcatagta
    18781 aagatcgcct ttttcatctg atgaaaccat ttgtcgcata cgagactcta cttctgcttt
    18841 tgagagacca gctgccggat cgctttgaag ttggatgcca agaccatgta tttcgattcc
    18901 tcgttctttg agtgccatcg cttctccgat ggttgcgata aacgtactgt cgattcttct
    18961 ggatagattg ttttggtcag gtgcatcata gattcttgag attagagaag tatttcccgg
    19021 ccgatcttgc gtattagaaa actgagttcc gtaataattg ctgcttgatt gtgcgtgtac
    19081 ccgctgtact ttataggaaa atgttggtac accatccgtc agcaaaacga tcactttttt
    19141 atgtccattt ggaacggata gcatgcttcc tgcatctctt agtgcttttt gtgtaaaagt
    19201 accaccattt gtccgtgaag gtgtaatgga ttttacttga tttttcactg aatcaaatga
    19261 acccatctgt actgcaccgt tactgtagct atatccttcg cttgagtagc cgacatatcc
    19321 catattgatt ttgtctgtga taccgctatc tgctaaagta tcgacaaaac gatcgacacc
    19381 aatctttact tcaccgatcc gattattgtc gttcatactt cctgaccagt ctacgaccaa
    19441 tacgagatca agaggcgtga tatctttttg tacatttcca cgagcattca aataaacatc
    19501 aaacaatccg ggtgtacttg tttcctttgc atactttcgt atcgcatagt ctgcttgatt
    19561 accggttcct ccatattcga tatacgaatg ggtccgatca ttaggatctc cattccaaga
    19621 atttatacca tcccattgat ttgttccgtt tttattgcct tgatgatcaa gaacatttgt
    19681 atttcctgta ggttgccaac tggctttagg ataagtgccc tgatcatctg ttgtatattc
    19741 tgtaggaatc gtttgattag atggcacact cacagctggc aaggataatg taggcaatgt
    19801 aaatgggctg acagaatttt cgttttcttt tgatgattct tcttctattt cgtctggctt
    19861 tggtactgac acatctgtat ctttcttatt ttctgtctca tcaataatgt catattcttt
    19921 attatcgacg atcagcttgt ttgcctggct gtggtaaagt gctggatcga ttggaaaact
    19981 cagatcgata gtttgtttag tgtttgatga gatttccaac gtaattttcc cgctatttac
    20041 ctgataagca tagccatctc cttcttttct ttccacatca cttaagtaat tttcgactag
    20101 accaatttct ttttctatct tttcagcact ggtgttattc gcatttagct tgatgttcag
    20161 tgcttttttt ccagactgat ccgaaatgac ttcagctgaa attgtttcta aaactgttgg
    20221 atcatctgta gtgtctgcta ttgcagataa tggcacaaag tttgataaac tcaatattat
    20281 cagcatgaaa ataactttca gtttcttccc tgtggtcgtc atttttcccc tcctttttct
    20341 ttttttctgt tttgacgtga atagtgaaaa ttattaaaat aaacaggcgt tacataattt
    20401 tatttatttt aatatgataa ttattatacg aagggcgcta aagggaatca caccaatttt
    20461 cataccaatt ggtttcattt atgaagttga ttccttcgat taatgagcgt ttcattgaaa
    20521 gaaaagggtt gagggagaaa aacatatgat caggttgttg gaaggaaact atagaaagat
    20581 ggaaaaaatc attcgaattc tgtccatttc tgacaagtgg atgactaaaa aagaacttgc
    20641 caaacgaatt ggtagttcag aatctacatt tgtaaggtat atcgaagaga tcaaaatccg
    20701 ctggggaacg gatgtaacga ttcaatcttc acaaaaattg gggtatcgtc ttgaacattt
    20761 gaatgtacca gcttatttaa atgttctgat ggaaatgtcg caaagttcta caaacagtcg
    20821 tttattaaat gagattattg tcaatccggg taaaacgatt gagtattatt gtgaaaaaat
    20881 ttccatcagc cggtcttctt ttacacgtaa gttgaaacaa tgcaatcaaa ttttagaaag
    20941 ctatttatta aaagtgatcg ttgaccaagg ttttcagtta actagcctta aaagtgagct
    21001 ccagttgcgt atctttgtta cctttttctt tttaagttac tacggaagac gcacgttgcc
    21061 atttccaatc gaaaaaaaga agatcaaaga aattatggtc aagaataatt gtcagatgaa
    21121 tttgtttcag gaagaaagcc catatgagca atctttcttt attatgtatt ttgttgtgca
    21181 tctcatgaga gaatcccaag gaactccatt tgaatggaaa gctggagact cttatgtaca
    21241 gttgataaat atcgataaaa aagacttctt aatcttgaaa aatctcttag gaaaattgaa
    21301 gtacagcagc tatataaaag tagtgaacta ttttgtcaat acgatttttt cagaattttc
    21361 ggagaaagaa aaaaatgaaa tcagtgagca gatacgaaaa aatatgtcct actgttcgtt
    21421 ttcatccgct gattgcaaag aaaaaaacga agaatatgtt gttcaattat taacgcaaat
    21481 ttattttttc tcccgtattt tcccatacga tacgactcat ttgacgatcc gtgcaactta
    21541 ctttgcacag cagatgcaac accgccagat acgtctatat gctggatttc gtgcagaact
    21601 tcaattttat tccactctac tgaatcagaa tctgatttat tatttatcta gctatatctt
    21661 ttggatgctt atcggtaatc ctgaaattca ccattatact tctgataaaa aaatactgat
    21721 tatcagcgac ttaagtcttc gacatagtca atatattgaa gaatatatca gtgatattct
    21781 tgctgtgcat aaaattcact ctgagacaac tgctattact gaagatcagc tgtcgaaata
    21841 caatcttctc gagtatgatc tgatcgttac gaatcaaccc attttgaatg cccatgttcc
    21901 tcatatattg attgatgata gtgtgtcatt cgcaaatgaa gaagaactca tccgcttatt
    21961 cgagttatga ataatcaaaa aacagctatg tataaaccac cctgataaaa atttcaggac
    22021 aaatacacat agctgtttct ttttattgta ttccttcagc aatccaacga gatttatagc
    22081 gaaagtcttt taacaactct tcttttgtcc aattatttgc aatgagatcc gtcaaagtgt
    22141 tcattacgag ggtcatggaa aagtatagga aactaactgc ttcttccgac gattgaatgg
    22201 agaaatgatt gattcttaat aattccagcc atttttcgtt cattggagaa tcagccgata
    22261 gttctctacg tttggttgtc tgctgatcat ttcgctgggt taacaagtat aacctttgcc
    22321 aatagatact ttcgtgatct aagtcgcagc accactctaa atatttttcg atccccaaaa
    22381 agaaatgttg tttgttttcg ctaatgaatt ttaagatatc ttgatggact atgtgagaac
    22441 tgcttttgat cgtatacaaa taagcatctt ccaaatcttg aaaatattta taaaaagcac
    22501 cgcgcgacat ccccatcttg tcaacgattt ctgagacttt gacttggctg atgtgctgtg
    22561 agcaaaatat ctccagtaat aggtcatcga ttctctgttt ctttgcgttc gataaattga
    22621 aaaaagtttc ttttggcatc cacatctctc ctttatctct gattatacgt ggatagccct
    22681 tcattgtcat gaaggattca ttccttcttt ttctcttctt ctgtcaagta cccatcttcc
    22741 attcgataaa cgcgatcact ccattgaatc atacggacat catgagtaac cataatagtc
    22801 gctttttgtt tctcatgagc ttctttaact agtaatttga cgacttcata tgcatgatct
    22861 gtatccaaac tagctgttgg ttcgtctgct aagatcaagc ttggctcatt atataaagcg
    22921 cgagcaattg ccactctctg tctttctcca ccagaaaggt ctttaggata actattttta
    22981 agtgactgga tatctaaaga ttgcagcaag gaatcgattt ttgttgaatc ttcttttttt
    23041 gaagtaactt ggtcaactag ttcaaattgt tcctttaccg ttaaaaaagg aatcaaatta
    23101 gagctttgga gaataaatcc tatttcttta aagcgtaaat ctgctcgttt tttttctgga
    23161 agctcagtta gtgagtgacc gttgatcact atttctccgg aagtcggtgt ttgcaatcct
    23221 cctgaaatgg taaggaaagt acttttacct gaaccagaag gaccaatgat gctgacgaat
    23281 tctccttcct ttacataaaa atcaattgct ttcaaagctg agacctttgt atgaccagaa
    23341 ccaaatgttt tggtaacttt tttcatcgtt aatatttttt tcatttttat taacctccta
    23401 ttgctgtaat tgggtctact ttggttatcg tacgtatcga aaacaatccg cctagcattg
    23461 cgacaatgat caaaatccca ctatatagca accattgtga ccaaaaaaca gcaaaaggca
    23521 tcgcgcttgg taaaatcaga ctagtcaaat aagcaaacaa aagagcaagc agaacgccta
    23581 ccaccccaac aataaaggac tgtgctacta aagatttagc gatgaagaaa tttttgatcc
    23641 cttgtgcttt cattactcca aaaatagcag tcttctgaag tgtcatcacg tacatgaaaa
    23701 taccgaccac ggcagcaacc actaagaaca aaaagtatat catcgcattt aaggtcatat
    23761 tttgggcaga atagccagga atactttcaa tgaatgtccc aatagttaat aactgtagac
    23821 ccttcgatgc tttttctttc gaaattttag ctgaatcctt tattacgatc ccattgatcg
    23881 gctgttcttc ttcagaagca aacggctgtt gtccatactt caaagcagtc cacgtatcta
    23941 gggacgcata gatgacggga gaaactgtgt aataagtttc aggaaagata ccgacaatcg
    24001 tcaaaggatc gtcgtaactg ccaattttga tcgtatctcc aattttatag cctgaatcag
    24061 ccagattttg agagatgatg atttcattct ttgttttaaa ttcatgccct ttcgtgagtt
    24121 ttggtaataa aaatgcttct ttcgttgtgc cgaaaactgt aacattttct ttgttttttc
    24181 ctttgattgc tccgctgtac aatccgattg gtgctttctc tcctccttca atgtatttca
    24241 gatctcctct tgttaattgt gaagctgcaa atacttgatt tgcatcctcc gacaaaacaa
    24301 tctcttgtgc gtcccaatca tcgattgctt ttttaaactc ttctgaaagt ccattcgcta
    24361 gtcctgacaa catgaatacg acatacgcaa tcaagaacat aataccgacg atcaagccat
    24421 atcgtaattt agaatagcga atttctttga tagctagaaa catttgtttt tcctccctat
    24481 gatagattga tagtgacaaa atgtcacttg ttgaatagtg taactctaaa gtgacaattt
    24541 gtcacttgtg ttgttttttt cttatcaata aattgtctaa gacatctttc gataaaacct
    24601 taacatgagt cataaaaaat ttatttaagc cttatttatg ctttccatga taaactaaag
    24661 tgaccaaatc aatgaggtgg gaaaatgatt aaactattag tagtagaaga cgataaagta
    24721 ttatccaata atataaaaga aatactcgct gcaatcggcg agataacaca agtttatgat
    24781 ggtagtgaag gactgttcga gttggaaagt ggagtttatg atcttgcaat cttggatttg
    24841 atgatgccgg aaatgaatgg atatgaagtg ttagcaaaaa tacggaaaga aaaaatacag
    24901 actccagtat tgattttgac tgcaaaagat agtttggaag ataagattga aggatttgaa
    24961 cgaggtgcag atgattatct aacaaaacct ttctatcgag aagaactgat catgcgcgtg
    25021 aaagcattat tgaaacgctc gctaggtctc tttgatgaaa atgttctgaa gtataaggat
    25081 atcagttgtg atttaaaaag caatgaagtc ttgtataaag aacaagtttt gcccatacaa
    25141 ggaaaagagt ttgaattgct cgtttatttt ttacaaaata agggaatcat tttgacaaaa
    25201 gagcagatat ttgaccggat ctggggattt gattctgata cgacgattac agttattgaa
    25261 gtttatatga gcaacttacg aaaacatctg cgaagtactg gattggataa agaaatcaaa
    25321 accttacgta atgtcggtta tatactgcaa agggtgtgat gcaatgaatg gagatattac
    25381 acgaaagcaa cgtagtcgct tttttgcgac aaatgtagca gcatttgctt ttatcttttt
    25441 tcttttggga ttcattactt tacagatatt aaaccaatct gcatacagag aaacagatct
    25501 gtcattagag aaaataaatt ttgattctcg aacagtacaa ttagagatac aaagatatca
    25561 gcaaggcgat ccctttttaa acccgactca aacggaacaa aacagaacaa acatggaaca
    25621 gccattaatg gaaccatcaa atgaccgttt taacactcaa gtgatcttgt ggtctaaaaa
    25681 tggtgagata ttgaataaag cagctattgg tgggcgtttc aaccaaatca aagaattaaa
    25741 aatggatacg aaaaatctga ataccatcca aaatattgaa ttaaatgaag acagtggctc
    25801 atctgccctc tctttccgtt cgattacgaa aaaagcggag acgaatcaag atggcgttgc
    25861 ctacatccaa attttagaaa atacaaatca aatcaagaat tcattagcga cttttcgcac
    25921 gattatgatt ttgtgtatga ttgttttttg gattctctct attggtatca gttattactt
    25981 gtctagtcta agcatgcgcc ctattattct ttcttggaga agacaaaaag aatttgttga
    26041 gaatgcttct catgaattgc gcacaccgtt aacgattatt caaaatagtt tggaacatct
    26101 ttttaccaag cctaatcata ctattattga agaatccgaa agcatcgctc aagcattgtc
    26161 agagacgagg cgcctcactg gattgacttc ggatttgctg acaatcgctc ggaatgattc
    26221 tgatcagcag ctgctttcaa agcaaatgat caatacacaa gaatatatcg aacaacttgt
    26281 taaaccattt caagagatgg ccatcataga tggaaaagag tttattctcc ataacgatgc
    26341 aacattagaa gtaccaattg atgaaaaaaa aatccatcaa gttttggtta ttttgctgga
    26401 taatgcatta aaatatacga atccagaaga taagatcatt ttggattcat ctgttaacca
    26461 taaagaatgg ctgattcacg taaagaatac tggcccttct atcagtgaag aagatcaagc
    26521 aaggattttt gatcgatttt atcgggaaga tcaatctcgg gcgaaagaga ctggcggata
    26581 cgggcttggc ttggcaattg caaaacaggt aatcgaacag catcatggac agatttctgt
    26641 atccaattat cttccacaag gagtcgattt taaagtccaa ttgccgttga agcctaaaaa
    26701 ataatgctta tccctaaaaa agggctgtga caaaaatctt gtcacagccc ttttttgttg
    26761 cgaataaacg gtgttccaga agtagcttct tcggaaataa gccgaaattt ctttaagctg
    26821 accacttctg tcacaacctc tttagcttac gcttacgatt ttttattgcg gtcttcaatg
    26881 tgtacatcac cgcttgtgtc aatatccaat tcttctcggc taacatcttc tgtcactttt
    26941 tcaacatctt catgttcttc tttatgaatc ccaacttctt cacgaataac tgtatgtttg
    27001 cttacatcta cttgctcttc tttgattggg atagtaatcg tgtcatcttc caatttttcg
    27061 tctgttccct gtgaagattg atctgttact ggtttgcgtt cgatgacgat ttcttcacgt
    27121 ttgaccggta cttctaccgt ttcagtatcg ttgaccacat gtttgtggat gtcgacttct
    27181 ccagttgtca catcatgtgt atttacatct aatttttctt cttttagttg aattgtatca
    27241 tcatctagat ttggtttgcg atcgatgtct tcagtcttgc ttgtacccat tgtatcagtc
    27301 tcgccaggta cgtccatcgg atcagttcct ggtgtcatat tcaatggatc cgttgtattt
    27361 tgaaagcctg tcgtgccacc tacgtccatt ggatctgttc cagtgccagt ttctaatcca
    27421 gtccccattg tgcttgctgg gctaacagta tcttgtgtat ccattgtatc ttctgttgtt
    27481 tctggctgac gatagccttt tactgtgatg acaaattttc catcagaaat atctcctcta
    27541 tattgatata aaggatcatt ttctggtgta gccgtctcag aatcataggt atcaaaagaa
    27601 aaagtatctt ttattttttc ccataaagaa cggtcttctt caccatccat ccgattttca
    27661 tctgtttcaa catctatacc agacaatccg tcaaatccta gtctagatgc tgcttcatca
    27721 gtagtataaa taacgatatc atctcgttca tacccttcgt tacgcaaccg ttcaattact
    27781 gataatgctt cttccctcgt actatagctt ccgatgaccg ttaaattttt ctcttccatg
    27841 taaattcctc ctagcaaata gatttatgat tggtatattg ttacttataa atttattcta
    27901 taggagacga ttgttttcta caaatattta gattggaatt tgattaaaac aatttaattg
    27961 aaat
//
LOCUS       DARBEC010000029        27951 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.029, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000029 DARBEC010000000
VERSION     DARBEC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 27951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 27951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.029"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCL80_001183"
     CDS             complement(114..473)
                     /gene="rplT"
                     /locus_tag="SCL80_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEG1107065.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCL80_001184"
     CDS             complement(526..726)
                     /gene="rpmI"
                     /locus_tag="SCL80_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEG1107066.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCL80_001185"
     CDS             complement(798..1322)
                     /gene="infC"
                     /locus_tag="SCL80_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEG1107067.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1366..1491)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1642..2274
                     /locus_tag="SCL80_001186"
     CDS             1642..2274
                     /locus_tag="SCL80_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1107068.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGDQSYLDAKN
                     MVLRESHIQKLTMQKTVLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTAIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2276..3181)
                     /locus_tag="SCL80_001187"
     CDS             complement(2276..3181)
                     /locus_tag="SCL80_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1107069.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWGIALIISLVLSIWMLIALWRRIR"
     gene            complement(3266..3925)
                     /locus_tag="SCL80_001188"
     CDS             complement(3266..3925)
                     /locus_tag="SCL80_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1107070.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     RVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVA
                     I"
     gene            4241..5185
                     /gene="mvk"
                     /locus_tag="SCL80_001189"
     CDS             4241..5185
                     /gene="mvk"
                     /locus_tag="SCL80_001189"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEG1107071.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5178..6155
                     /gene="mvaD"
                     /locus_tag="SCL80_001190"
     CDS             5178..6155
                     /gene="mvaD"
                     /locus_tag="SCL80_001190"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEG1107072.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKQGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6168..7253
                     /locus_tag="SCL80_001191"
     CDS             6168..7253
                     /locus_tag="SCL80_001191"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEG1107073.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7250..8314
                     /locus_tag="SCL80_001192"
     CDS             7250..8314
                     /locus_tag="SCL80_001192"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEG1107074.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKVPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAKEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8563..9366)
                     /locus_tag="SCL80_001193"
     CDS             complement(8563..9366)
                     /locus_tag="SCL80_001193"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEG1107075.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIPDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCL80_001194"
     CDS             complement(9386..10228)
                     /gene="kduI"
                     /locus_tag="SCL80_001194"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HEG1107076.1"
                     /translation="MQEMETRYTHSPEDIRHYSTEQLRKEFLVEKVFEPGKISLTYTH
                     NDRMIFGGVTPTEKELEITLDKELGVDYFLERRELGVINIGGPGFIEIDGQKEEMKKQ
                     DGYYIGKETRHVVFSSQDAENPAKFYISSVPAHHKYPNVKISIDQVKPMETGEGLTLN
                     ERKIYQYIHPNVCESCQLQMGYTILEPGSAWNTMPCHTHERRMEAYVYFDYANEDTRV
                     FHMMGKPDETKHLVVDNEQAIISPSWSIHSGVGTSNYSFIWAMCGENITYTDMDMVQM
                     DQLK"
     gene            complement(10244..11251)
                     /locus_tag="SCL80_001195"
     CDS             complement(10244..11251)
                     /locus_tag="SCL80_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1107077.1"
                     /translation="MGKVVTLGEIMLRLSTEQGTRIDSSERFAAHYGGGEANVAISLA
                     NYGHEAIFATKVPDNALGKAVKKHLQCYGVDTKQVLSGGSRLGTYYMETGSGERAASV
                     IYDRAGSSFAEMDHLEWSLEELFQGIDIFHVSGITPALSKKWQKMTKQVIEAAKKAGC
                     LISFDINYRGKLWTQQEASEVIHQLLPLVDICSAGKMDALYLLGIEKAPEAEEQPLIY
                     YYQKMQEKFPNIQVFYSTKRTVHSASENELKGTLWMNHEYVESQVHRITPIVDRVGGG
                     DAFAGGVLHGILSNMSPQEIIDFATAASALKHTIHGDCNQFSQEEVAQFLACGSGKII
                     R"
     gene            complement(11258..11902)
                     /locus_tag="SCL80_001196"
     CDS             complement(11258..11902)
                     /locus_tag="SCL80_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1107078.1"
                     /translation="MKKIDILSRLKNAGVIAVVRGKSKEEALNACHAIIKGGLTGIEL
                     TFTVPQADQVIKELLSFYKDQPEIVIGAGTVLDAVTARLAILAGAEYIVSPSFDQETA
                     EMCNLYQIPYLPGCMTITEIKTALKSGVDIVKLFPGSAYGPSIISAFKAPMPQVNIMP
                     TGGVSLDNMKEWFDAGVVTVGVGGNLLAPAATGDFDKVTEVAQQYAAKMKEIKR"
     gene            complement(12002..12760)
                     /locus_tag="SCL80_001197"
     CDS             complement(12002..12760)
                     /locus_tag="SCL80_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HEG1107079.1"
                     /translation="MENKKPYGTVLIKASKILDYLAENQDVSLQTIAKGVGMTSSTVL
                     KILDTLLMIGYVNKNSEKNYRLGAKLIRYANKNIEQIDLVELTLPFLEQLQQKIDETI
                     HLGVLDNNEILYVNKLDPKHQTIRMSSKIGITRPLYNSAMGKAVLAEFSEEQYTDYLE
                     KHPLIPYTEYTITNALRLKKEIKTVKETHIAFDDEEIERDIFCIGASIVKNEQIIGAF
                     SVSMPKYRLTDETKEQIISALKQTKSEIEQRIQK"
     gene            complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCL80_001198"
     CDS             complement(12887..14038)
                     /gene="fucO"
                     /locus_tag="SCL80_001198"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HEG1107080.1"
                     /translation="MSNRMILNETSYFGKGAIESIVPEFQQRGFGKAAVITDKGLIEH
                     GIATKVTELLDKAAIAYALYDGIVPNPTIQNVKDGVAFVKEAEADCLIAIGGGSPIDT
                     AKAIGIILTNPEFSDVISLEGVADTKNPCLPILAIPTTSGTAAEVTINYVITDEVNHR
                     KFVCVDPHDIPIVAFIDSDMMMGMPKKLAASTGMDAMTHAIEGFITKGAWEMTDMLHL
                     KAIEIIGHSLEASVDGDQNGREKMALGQYIAGMGFSNVGLGLVHGMAHPLSAWYNIPH
                     GVACAALLPTVMKYNKEYTGEKYREIALVLGIKGAAEMSLEDVREAACGEIDRLSKAV
                     GIPETISELGVKEADIPAIAEDALRDVCTPGNPRETTVEEIIALYQSLM"
     gene            complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCL80_001199"
     CDS             complement(14120..14431)
                     /gene="rhaM"
                     /locus_tag="SCL80_001199"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HEG1107081.1"
                     /translation="MVKKAFCMKVYPEKQEEYKKRHDELWPEMRTALREHGVIDYSIY
                     LDPSTSLLFGYLVIEDEKLWEKMANTSINQKWWKYMEDIMETNPDASPVSWELMPVFE
                     L"
     gene            complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCL80_001200"
     CDS             complement(14433..15278)
                     /gene="rhaD"
                     /locus_tag="SCL80_001200"
                     /EC_number="4.1.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
                     /protein_id="HEG1107082.1"
                     /translation="MKKIDILQAPYVKEMIETTSNLYRLGWDERNGGNISYLLKEEEI
                     TPFLDPEKVIRRISMIFDASRLAGCYFIVTGSGRYFKNVASEPAENLGIVRVAEDGKT
                     LELLWGLENECLPTSELPSHFMSHIARLEVDPDNRIVMHCHASHLLAMSFTHELDERS
                     FSRTLWQMCTECLVVFPEGISIIPWMVPGTNEIGEATAEKMKETRLVLWPQHGIYGSG
                     KDMDEVFGLIETAEKAAEVYTYVKAQGPILQTITDENLWHLADAFGVTPKAGYLEEVH
                     TKAGV"
     gene            complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCL80_001201"
     CDS             complement(15292..16548)
                     /gene="rhaA"
                     /locus_tag="SCL80_001201"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HEG1107083.1"
                     /translation="MNTIERNYEQAKEKYATIGVDTDAVLEKIQDIKISMHCWQGDDV
                     KGFLTPDGELTGGIMATGNFPGAARTPEELRQDLEKAYSLIPGKHKLNLHAIYLDTEE
                     SVDLNEIEPKHFEKWVEWAKKEEIGLDFNPTFFSHPMMKDGFTLAHPDKEVREYWIEH
                     GKRSRKIAEYFGKETGQTSINNFWVPDGMKDNPIDRFTPRERLMESLDEIFTEELDER
                     YVQDAVESKLFGLGAEAYTVGSHEFYMGYGLTRDKLVCLDAGHFHPTEVISNKLSSLA
                     LFSKGIMLHVSRPVRWDSDHVVLMDDELQEIAKELVRNELLEKTNIGLDFFDATINRI
                     AAWVIGTRNTQKALLKAMLEPVERLKEMELAFDFTSRMAYTEELKDFPYADVWNYFCY
                     KNQVPVGLDWLEEVQEYQKDVLELRK"
     gene            complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCL80_001202"
     CDS             complement(16545..18005)
                     /gene="rhaB"
                     /locus_tag="SCL80_001202"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HEG1107084.1"
                     /translation="MNPTFVAIDIGASSGRLMKSTRDAKGSLILEEVHRFKNGFRNKD
                     GHDRWKIDYLIKEILIGFEKLKQTGIDRLFAGIDTWAVDYCLIDEQGDRINDPIAYRD
                     KRTNGAVEQFSEIYSLDKLYEQTGIQIQPFNTLFQLFAEEKENLAKAHKLLLIPDYLG
                     YVFTGNMVTEKTNASTMQLLNAGTKEWEIELLEKIGVSKELFPPLTEPGTILGDLQKE
                     QFSKYDLPDVTFITVASHDTASAVLGTPGQGERWGYISSGTWSLLGIETKIANVSRNA
                     FSENYTNEWGAHNTVRFLKNIMGMWLIQEVARMQEYEYSYAELAELAAKEKPLQQVME
                     INDPRFLNPPNMIEEIQQYCFETEQPVPVSSGAIARCVYDNLALCYAQELIKVEHLTG
                     TKLEKLHIVGGGSNNRLLNQLTADYAGIIVEAGPSEATAIGNVAMQMLSTGQFSNIEQ
                     ARNVIKQSFPCETFYPDENKQNTMEWYVNKIKGVIV"
     gene            18158..19027
                     /locus_tag="SCL80_001203"
     CDS             18158..19027
                     /locus_tag="SCL80_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family ligand binding domain-containing
                     protein"
                     /protein_id="HEG1107085.1"
                     /translation="MDYWEKANHSWSEDSKRVILTPTQRSRNLFFYIQEIGYFKAPIP
                     YYTERAHLPSYLIKFTLSGKGSLTYNDNQQQLKAGDLFFIDCQEYQRYQTISEEPWEM
                     DWIHLNGQNVQSFYQEFTKDGHNTFHSTGDPSENKIHHLITELIHMQQGTNARTDFQS
                     SVAIHELLNELILQKYQLDFEEEDIPAYVYKLKQYLDEHFKETITLDQLEQQFLLNKY
                     QLSKDFSKFIGSPPIDYLISKKLSYAKDLLRYTEFTVRMISEEIGIENFAYFSRLFKA
                     KTGLSPRDFRKFS"
     gene            complement(19153..20052)
                     /locus_tag="SCL80_001204"
     CDS             complement(19153..20052)
                     /locus_tag="SCL80_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107086.1"
                     /translation="MLQKKISGEQVNTMVSDQYEIYHVKDVVSQNKTIYHHHDFYEIH
                     ATLKGEATFYLNGRQFTIKAGSVLLIHSNDLHRIVRQSSDVFERVYIFVTPSFLESRS
                     TPWSNLSACFQPMGEKRSRVLQIEQEVLKQKLSFIDHPPDKKIYGEDIKYEQALVEYL
                     IFLNQVVQKEESAQEQNAVVPNERVEQMLQYISKNLSHPLSLEEMEKQFFVSKYHITR
                     EFKKYTGFTFHQYVMKKKLIYAKHLLREYRSSSAVYSKSGFSSYPHFLKAFKREFGIT
                     PKEFLKRDTQKQLVHYEHFEDEQ"
     gene            complement(20217..21041)
                     /locus_tag="SCL80_001205"
     CDS             complement(20217..21041)
                     /locus_tag="SCL80_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEG1107087.1"
                     /translation="MLNITIRGHDLSEVQTIEDLAEKTKEQGIHTLQLALGLSFPEIP
                     SGANEINSGMGNYVKRVLEKQEVTVGILSCYINMIHPDLTIRQELLTKFEQYLRYASS
                     FGASMVASETGCVLPEIQYTEENFTDEAFAEAVSVIRRLVKAGEKYQMMVGIEPGLNH
                     PVYSLARVEQLIQAVDSDYLGIILDPTNLITSSDYQEQVQLVEEAFERFGEKICAVHL
                     KDFRVEQEKIVPVNLGDGVIEYTKIKEIIMKNRPYLYVVLEETKDDGIRYGRSLLE"
     gene            complement(21052..22347)
                     /locus_tag="SCL80_001206"
     CDS             complement(21052..22347)
                     /locus_tag="SCL80_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease"
                     /protein_id="HEG1107088.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGALWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKI
                     IKEAIPSLLLIVIIIGGILTGIFTAVEASAIAVIYSLLIAMFYYKTVKLKDIPNMLFQ
                     SVAMSGTIMFLLATSSMMSFAMAFTGIPQAISSLIMGVTDNKFIILLLVNLVLLLVGM
                     FMDVAPAILIFTPIFLPIVTSVGVDPVHYGLFSIMNLCVGSITPPVGTGLYVGASVGG
                     VKAEAMLKPLLPFYGVILVVLFLITYFPQIVMWLPNMLS"
     gene            complement(22363..22842)
                     /locus_tag="SCL80_001207"
     CDS             complement(22363..22842)
                     /locus_tag="SCL80_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEG1107089.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKAHLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(22848..23876)
                     /locus_tag="SCL80_001208"
     CDS             complement(22848..23876)
                     /locus_tag="SCL80_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
                     /protein_id="HEG1107090.1"
                     /translation="MKKTSLLFAASSLSLGLLGLSGCGNNSSVATVADADVNEIVTLR
                     FAYASNSQPVIDAMNEFGRLVKEKTDGSVQIQYFPDGQLGGERELIELTQSGAVDFTK
                     VSASALESFSKDYSIFSVPYLFNDEEHFFKVMDNEEIMEPIYQSTKNLGFTGLTYYDS
                     GQRSFYMVDGPIHTPEDLKGKKIRVMQSETAIKMVELLGGSPVPMGSDEVYTSLQSNL
                     INGSENNEFVLYTAGHGGVAKYYSYDEHTRVPDIIIMNDAIKEQLTDEQEKAIEEAAK
                     ESTVFEIEAFAKAIEEEKALAQEEHGVQFNDVDNTPFREAVAPLHDIFKNDPTYKDLY
                     QKIQEQGA"
     gene            complement(23893..24999)
                     /locus_tag="SCL80_001209"
     CDS             complement(23893..24999)
                     /locus_tag="SCL80_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1107091.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCL80_001210"
                     /gene_synonym="adhC"
     CDS             complement(25228..27825)
                     /gene="adhE"
                     /locus_tag="SCL80_001210"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEG1107092.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
BASE COUNT         7883 a         6132 c         5041 g         8895 t
ORIGIN      
        1 tattttagac ttatacgtta ttgtcctgtt tcatttgatg gaggattgta aataaaaaaa
       61 gaggaaagaa cataactttt cgttatcttc tttcctcttt ataattgttt agcttattta
      121 gccaaagctt cttttgcttg gtcagccaat gcagtaaatg ctgctgcatc gttaacagcc
      181 aagtcagcta acattttacg gttgatgtcg atttcagcaa ctttcaaacc gtgcattaat
      241 tttgagtagc tcaagccatt catacgagct gctgcgttga tacgagcaat ccacaattta
      301 cggaagtcgc gtttcttttg acgacgatcg cgatatgcgt agttgtatga attcattact
      361 tgttcttttg ctgttttaaa taaagtgtgt tttgaaccgt agtaaccttt tgctaatttt
      421 agcactcttt tacgacgttt acgagttact gttccacctt taacacgtgc catgtataat
      481 tcctcctaat aaaaataaat cttcagattc tgttatttgt acttcttatt tcatttttgc
      541 tagttgttgg cgaatacgtt tgaagtcgcc actagaaacc atgcttgctt tacgcaattg
      601 acggcgttgt ttttttgttt tgccgtggaa acggtgactt gtaaacgcgc ggaagcgttt
      661 caatccgcct ttaccagtac gtttgaaacg ttttgctgat ccgcggtgtg ttttttgttt
      721 tggcatgact atatttcctc ctcaaaatct ttcgttatcc gactatcggc tgtccaacag
      781 ccagttagtc attcaaatta cttactgtcg tttttcggtg ccagcgtcaa gaacatgctg
      841 cgtccgtcca ttttcgcttt ttgttccact gttgcaatat ccgcagtttc ttcagctaag
      901 cgattcaaga ctttttgacc aatttcttta tgggtaatgg cacggccttt gaaacggata
      961 gaagctttca ctttatcgcc cttttccaag aacttacgtg cattacgaag ttttgtattg
     1021 aagtcattca agtcgatcgt aggacttaag cgaacttctt ttacattgat cactttttgt
     1081 tttttacgcg cttcgcggtc tttcttttgt tgttcgaaac ggtatttacc gtagtccatg
     1141 attcgcgcaa ctggtggttt cgcaccaggg gcaactaaca caagatccaa gtttgcttgt
     1201 tctgcaatat tcaatgcttc tgcttttgtc ttaactccta attgctcacc atctgaaccg
     1261 atcaatcgta actctcgtgc acgaatgccg tcgttaacca tcatatcctt tgctatggtc
     1321 attcacctcc aaatattatg agagaaagaa aagcttaccg aataaacggc aataaaaaaa
     1381 cgagttctct tacagaaccc gcgcgtactt cacctaatag aaccagtgaa aactatctag
     1441 cccagtgaca ttatcaacta aggcgagaag cgggtgcttc tgcttttatt tctcaaccat
     1501 catagtatag caaaaactaa tctttttgtc aatgtttttc atacagtttg tcttcctttt
     1561 ctgaaaatga ttagattagc agactttcta gcagtgattc ccgcttcttt gctatactaa
     1621 agaaaaacga ggtgaaccca catgaaaaaa atcgaaccta ttccaccttc tcttcctctt
     1681 cttacaaagg gagagtttta tttagaagat gaggagatct tctctggtct tctgatcaac
     1741 gggggtgacc aaagctatct cgatgccaaa aatatggtat taagagagag tcatatccaa
     1801 aaacttacga tgcaaaaaac agttttacgt cattttgaat gcagtaatgt catttttgaa
     1861 aaatgtgatt tttccaatct ggagtggatt ggtggcagtt tccaccaagt cgtgtttcat
     1921 caatgtaaat taacaggtac gaattttgcc gaaagttatc ttcgagactg tactttcact
     1981 gattgtattg cttcgatggc ttcctttagt agtacaaact tgaaagctgt ctccttcgat
     2041 cattgtcaat tagaagacag cgaattttat gaggtcactt ggaaaaacct ttttttgtct
     2101 gacaatcagt tgaccggttc gaactggttt cgaacttctc tgaagggatt agactttacg
     2161 actaatttct tcacagcaat cgctttatct caagattctt tggcgggact aatcgtcaac
     2221 caagagcaag cacttgtgat cgcccaagcc cttggtttgc agatcaaaga ctagatcaac
     2281 gaatccgccg ccacagtgca ataagcatcc aaatagataa gaccaaactg atgatcaagg
     2341 cgattcccca tccgaaaacg gaatgggtaa agggtaactg gagattcatt ccgaagaatc
     2401 ctgtcacaat agttggaacg gtcaagatca gcgaccagac cgtcaaaaat ttcatcgtgt
     2461 cattcaagtt gttattcagc aaattattat aagtgccaga gagctggtct aaaatctgcg
     2521 aagctaagtt cgtcatttca actgcttgtc gtgcttcgat cagcgcatcg tccaactctt
     2581 ctttttcctt ttctgacaac ttgcgataaa tagccaatgc cttgatttgt tctaaaagta
     2641 ctacattttg ttttgttcct gtaactaagt aaaccaaacc gatctctaaa tctgaaagtt
     2701 gcagcagatt cttgttcgtt gttttttctc ttaatttcag attaagccgc tgacgttcac
     2761 tattcacttc ctcgattaaa ggaaaaaagg aatcagaaat caaaaacaat gtacgaaaca
     2821 ataaactgta tacactcatg tctggcattc gttccaaaag cgatcgaatt agtcgaatca
     2881 cgtactcggt attcggcgtg gtaaatgtaa acaaaccgtc ttcttttaaa ataaaagcga
     2941 taggactagt ttcataatga ttatcgactt tttgctggtg cggaacattt gatacaagca
     3001 gtaacgcttc atcaaaagaa tcgtactcta cgcgcgcccg ttcattttta tctagagaat
     3061 aagtcagcat ctcgtcacta atgccatatt cttcttttaa gtttttaaca gctagcacat
     3121 cgttttgctg tacattgatc caaaaattac ggtcttgatt aaaatagtgt ttttcccgca
     3181 tactctatcc tcctgacatt tgtttccagt ataaagaaaa aagtcgctca aataaaggtt
     3241 taccctttat ttgagcaact gtaaatcaaa tggctacttt ggtttggcga atctctgcta
     3301 atttttcttc gatcattttc aagacttctt cttttgcttg aggatcttcc acaaaattat
     3361 agcggtctcc atcgatttgg atttttggac tttctttgta ttcttcatac cattgatcgt
     3421 aacgtctgtt caattctttg taataatcgt atagacttgg atcaaaggac aattgttcat
     3481 acgaacgacc acgtttttcg atacgctcca acatcgtatc aaaagataca cgaatatgaa
     3541 ctaataaatc cggatgtttt ttatgggcag catagggaag ttcttccatc atgttttcca
     3601 gcagttcatc atacacacga acttctgttt ctgttgctct tcctagatct gcatttagat
     3661 ggaaaagcag tgaatcttca taaatcgaac ggtccaatac attattgtca tctttcattg
     3721 cttgtttgat gcttgcaaaa cgtttattca aaaaataaat ttgcagcaaa aaagcatact
     3781 gttcagggtt tgcataaaat aagggcaaga cttcattatc atcaatcgat tcataaaatg
     3841 cctgactatc aaagtgttca gccatcattt ctgttaaact tgattttccg gcaccaattg
     3901 tacctgctaa aacgatcacg ctcatttacc tccagctcac ttcatcacta aatattgtgt
     3961 ttaactccaa cctttttaaa tataccccca atatctagta gtgtcaattg tatttttttc
     4021 tagtcaaaga agaaaaaaag tcaaaaaatt cttaagaaaa aagtgttttt tctttagaaa
     4081 acgtattctt agttttactt ctgagatggt tttagtaaaa taaaggagga atgttttttg
     4141 agacccttag tggacaaaaa aattcgtgaa gcagcgcaac tcgttttggg cgctttattt
     4201 tttgcttaaa aatcactttt acgtgagaag gagttagatt atggcaaact atggccaagg
     4261 agaatcaagc ggaaagatca tattgatggg cgagcacgcg gttgtttatg gagaaccagc
     4321 gattgccttt cctttctatg caacaaaagt caccgcattc cttgaagagc tggatgcaat
     4381 ggacgatcaa ctggtttctt cctactattc aggaaattta gccgaagctc ctcatgcatt
     4441 aaaaaatatc aaaaaattat tcattcactt aaaaaaacag catgacatcc aaaaaaactt
     4501 gcaactgacc attgaaagca cgattcctgc tgaacgtgga atgggatcaa gcgctgcagt
     4561 cgccacagca gtcactcgtg ctttttatga ttacttagca tttcctttgt ctcgtgaaat
     4621 actattagaa aatgtccagc tttcggaaaa aatcgcccac ggtaatccta gtggaatcga
     4681 tgcagccgct actagcagct tgcagccgat ttattttaca aaagggcatc ctttcgacta
     4741 cttttctttg aacatcgatg cttttttgat tgtcgctgat acaggaatca aaggacaaac
     4801 aagagaagcc gtcaaagatg tcgctcacct ctttgaaacc cagcctcatg aaactggaca
     4861 aatgatccaa aaattaggat acttgacgaa gcaagcaaaa caagcaatca ttgaaaattc
     4921 accagaaacg ttggcacaga caatggatga atcacaatca cttctggaaa agctgacaat
     4981 cagcaatgat tttcttaatc tattgatcca aacagcaaaa gataccgggg cattgggtgc
     5041 caaattaact ggcggtggac gcggtggctg tatgattgca ttagcacaaa caaaaacaaa
     5101 agcccaagaa atcagtcaag cacttgaaga tgcgggtgct gctgaaactt ggatacaagg
     5161 attaggagta catacctatg tttaaaggca aagcacgcgc atataccaac attgctctaa
     5221 tcaaatattg gggtaagaaa aatgaagaac tcatccttcc aatgaacaac agcctttcgt
     5281 taacattgga tgcattttat acagagacag aagtcatctt ttctgatagc tatatggtag
     5341 atgaatttta tctagatggc accttgcaag acgaaaaagc aacaaaaaaa gtcagtcagt
     5401 ttcttgacct ttttcgtaaa gaagccggcc tctcactaaa agcttcagta atcagtcaga
     5461 atttcgttcc taccgcagct ggattagcct cctctgccag cgggctagct gctttagcag
     5521 gcgcttgcaa tactgctctt aagcttggat tagatgatct ctctctttca agatttgctc
     5581 gacgcgggtc tggttcagct tgccgaagta ttttcggtgg tttcgtcgaa tgggaaaaag
     5641 gccatgacga cttaagttct tacgctaagc cagtcccttc cgattctttc gaagacgatt
     5701 tagcaatggt tttcgttttg atcaacgacc agaaaaaaga agtgtccagc agaaatggga
     5761 tgcgtcggac agtcgaaaca tccagttttt atcaaggctg gttagattcc gttgaagggg
     5821 atctatatca attgaaacaa gcaatcaaaa caaaagattt ccaacttctc ggagaaacga
     5881 tggaaagaaa cggactaaaa atgcatggaa cgactttagc tgcccagcct ccattcactt
     5941 actggtctcc tgacagctta aaagcgatgg atgcggtgcg acaattgaga aagcaaggca
     6001 ttccatgcta ctttacgatg gatgctggcc ctaatgtcaa agttttagtg gaaaacagcc
     6061 atttatctga ggtacaagaa acatttacca aattatttag caaagaacaa gtaattactg
     6121 cacatgctgg tccaggaatt gcgattattg aataaaggga gatttttatg attgaagtat
     6181 ctgcaccagg caaactctat attgccggag aatatgcagt tgttgaaaca ggccatccag
     6241 cagttatcgc tgcagtcgat caattcgtga cagtcactgt agaatctgca cgaaaagtcg
     6301 gaagtatcca atctgcacaa tatagtggga tgcctgtacg ttggacaaga cgcaacggag
     6361 aattagtttt agatattcgg gaaaatcctt ttcattatat ccttgctgct attcgcttga
     6421 ctgaaaagta tgcacaagaa aaaaacattc ttttatcctt ttatgatctg aaagtaacaa
     6481 gtgagttaga cagttcaaat ggccggaaat atggattggg ttctagcggt gccgtgaccg
     6541 ttgcaacggt caaagccttg aatgtttttt acgcgttgaa tttatctcag ttggagattt
     6601 tcaagattgc agcactagcc aatttagcag ttcaagataa tggttcctgc ggcgacatcg
     6661 ctgctagctg ttatggtggc tggatcgctt tctcaacctt cgaccatcct tggctccaag
     6721 aacaagaaac tcagcattct atcagtgagt tacttgccct ggattggcca ggtctatcca
     6781 ttgagccatt gattgctcct gaagatttac gtttattgat tggttggacg ggtagccctg
     6841 cctctacttc tgatttggtc gatcaagttc accgttcgag agaagataaa atggtggctt
     6901 atcagctttt cttaaaaaat agtacagaat gtgtcaacga aatgatcaaa gggtttaaag
     6961 aaaataatgt aacgttgatc caacagatga ttcgaaaaaa ccgacaatta ctgcatgatt
     7021 tatctgcaat cactggggtc gtcatcgaaa cgcctgcttt gaacaaattg tgtaatttag
     7081 ctgaacagta tgaaggagcc gcaaaatctt ctggtgcagg tgggggcgat tgcggaatcg
     7141 taattgttga ccagaaatct ggcattcttc ctttaatgag tgcatgggaa aaagcagaaa
     7201 tcactccact gccgttacat gtctatagcg atcaaagaaa ggaaaaccga tgaatcgaaa
     7261 agacgaacat gtttcactag ctaaagcctt tcatgataaa caaaaaaacg aatttgactt
     7321 cgtacggatc atccataacc ctttgccgca aatagcagtt tctgatgtcg atctaagtac
     7381 acaggcagtt gggtttacac taagcagccc attttatatc aatgcgatga ctggcggaag
     7441 cgaaaaaaca aaaaaaatca accaagatct tgcaatcgtc gcaagagaag cagatttgat
     7501 gattgccact ggttcagtca gcgctgcctt gaaagtccct tctttagccg atacttatac
     7561 gattatgcga caagaatatc ctcacgggaa aatcattgcc aacatcggtg cagggacttc
     7621 tgtcgaaaga gccaaagaag ctatccgatt gtttcacgca gatgccttac agattcattt
     7681 gaatgcacct caagagttgg ttatgcctga gggagaccgt gattttacca attggaaagt
     7741 acttattcaa gaaactcaaa cagccattga cgtccccctc atcgtaaaag aagtcggttt
     7801 tggcatgaca agagaaacgc tcaatgatct agctgcttta ggagttcata cagttgatat
     7861 cagcggtcga agcggaacaa gcttcaccca gatcgaaaac gcccgtcgct ccaagcggga
     7921 actgagttat ttagctgact gggggcaatc gacagtctct tcattgcttg aagcaaatga
     7981 agcagacact tctatggaaa tcctagcctc cggaggtatc cgcaatgcct atgacatatt
     8041 taaagccctt tgtctcggcg caaatgccgt gggtacttca ggaaccgtac tgacgcatct
     8101 gatgaatcac ggcgtagaag aaactattat actgatgaag caatggcaag aagagcttcg
     8161 tctgctttat actatggtcg gcgcaacgaa taccgcggcc ctacaccaac aatctctgat
     8221 cttttccgga ccagtgaagg attggtgcga agcaagaggg atcgacttag tgaagtatgg
     8281 aagacgcaca gaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa
     8341 cacgtacaaa cggcggaagt aaaagcaact ccttcggaaa taagctgaaa ttcacaaaaa
     8401 tttgaaaagc aattttcgtg aattccttct tatttcttgg agttaaatgc ttctgtccca
     8461 gcctcttttt tcgaataaat gatgtttaat cagctgtcct tcggtattcg ttcaatccgc
     8521 tgatttcaca acctctccac taaacagtgt actaaagctc ttttaaggtt gttttccgat
     8581 ataggcaagt atgcctccgt ccacatacaa gacatggccg ttaacgaaat cagatgcgct
     8641 agaagccaag aagacagatg gtccagccaa atcgattggc tcgccccagc gtgctgctgg
     8701 cgtacgtcca acgataaatt gatcgaatgg atgacgttca ccgttttctt gtgtttcgcg
     8761 taatggagca gtttgcggtg tagcaatata acctggtcca attccattac attggatatt
     8821 gtattgaccg tattcagaag cgatgttttt cgtcagcatt tttaatccgc cttttgccgc
     8881 agcataagca ctgaccgttt cgcgtcctaa ttcactcatc atggagcaga tattgatgat
     8941 tttaccgccg cctttttcga tcatatccgg aataactgct tttgaaacga tgaatggggc
     9001 atttaaatcc acgtcgatta cttggcggaa gtctgccgca tccatttcga tcatcggtgt
     9061 ccgtttgatg atccctgcat tgttgactag aatatcaact gaacctacat cttctttgat
     9121 ctgttgaacc atcgcttgaa ccgcttcttc atcggtcaca tcgcagacat aaccttttgc
     9181 atcgatgcct gcttctttat aatgcgctat accttcatca actgattctt gattcaagtt
     9241 attaaatacg atttttgctc ctgctgtagc taaagagcgt gcaatctcaa atccaatacc
     9301 atagacagca ccggtgacta atgctacttt tccatctaaa cgaaacatat ccatactaaa
     9361 ttccatcatt ttctctccct tatgcttatt ttaattgatc catttgcacc atatccatgt
     9421 ctgtataggt gatattttct ccgcacattg cccagatgaa tgagtaattg cttgtaccta
     9481 cacctgaatg gatcgaccag cttggcgaaa taatggcttg ttcattgtct acaactaaat
     9541 gttttgtttc atctggtttg cccatcatgt ggaacacacg tgtatcttca ttggcataat
     9601 caaagtagac ataagcttcc atacgacgtt catgtgtatg acaaggcatc gtattccaag
     9661 cgcttcctgg ttctaaaatc gtataaccca tttgtaattg acagctttcg catacatttg
     9721 gatggatgta ttggtagatt ttacgttcat tcagtgtcag gccttcacca gtctccattg
     9781 gttttacttg gtcgatactg atttttacat ttggatattt atgatgagct ggaacagaac
     9841 taatatagaa tttagctggg ttttcagcat cttgagaaga aaatacaaca tgtcgtgttt
     9901 cttttccgat atagtatccg tcttgttttt tcatttcttc tttttgccca tcgatctcga
     9961 tgaatcctgg tccaccgata ttgatcacac ctagttcacg acgctctaag aagtaatcaa
    10021 cacctagttc tttgtctaat gtgatttcta attctttttc agtaggtgtc actccaccaa
    10081 agatcattcg atcgttgtgc gtataagtca aactgatttt ccctggttca aatacctttt
    10141 ctactaaaaa ttcttttctc aattgttccg ttgaataatg acggatatct tctggactgt
    10201 gtgtataacg tgtttccatt tcttgcattt tcttctactc cttctatcga atgatttttc
    10261 cagagccgca tgctaaaaat tgtgcgactt cttcttgact aaattggttg cagtccccat
    10321 ggatcgtatg cttcaaggca gaagctgccg tagcaaaatc aatgatttct tgcggagaca
    10381 tattggataa aatcccatgg agcacgccac ctgcaaaagc atctccgcca cctactcgat
    10441 ccacgattgg ggtgattcta tggacttggg attccacata ttcatggttc atccacaatg
    10501 tccccttcaa ttcattttca cttgctgaat gaacggtacg cttggtcgaa taaaacacct
    10561 gaatattcgg gaacttttcc tgcatttttt gataataata aattagaggt tgttcttctg
    10621 cttctggcgc tttctcgata cctaacaaat aaagagcatc catttttcca gctgagcaga
    10681 tatctactaa aggtaaaagc tgatggatta cttcgctggc ttcttgctgt gtccataatt
    10741 ttccacgata gttgatatcg aagctgatca ggcagccagc tttttttgca gcttctatca
    10801 cctgtttcgt cattttttgc cattttttag acaaagctgg ggtgattcct gaaacatgaa
    10861 atatatctat tccttggaat aactcctcta gcgaccattc caaatgatcc atttcagcaa
    10921 aacttgatcc ggcacgatca tagatcactg aggcggcgcg ttcgccacta ccagtttcca
    10981 tataataagt acctagtctt gatccaccag ataatacctg tttcgtatct actccataac
    11041 attgcaagtg ctttttgact gcttttccca atgcgttatc aggtactttt gtagcaaaaa
    11101 tcgcttcatg tccgtaatta gctaaagaaa ttgctacgtt tgcttcccct ccgccataat
    11161 gggcagcgaa tcgttcactt gaatcgatac gggttccctg ctctgtggac aatcgcaaca
    11221 tgatttctcc gagtgtcact acttttccca taagcagcta cctcttgatt tctttcattt
    11281 ttgctgcata ctgttgcgcg acttccgtta ctttgtcaaa atctcctgta gctgcgggtg
    11341 ctaaaaggtt cccacctact ccgactgtta cgacaccagc atcgaaccac tctttcatat
    11401 tgtccagact cacaccgcca gttggcatga tattgacttg aggcatcggc gccttgaagg
    11461 cagaaatgat acttggtcca taagcgcttc caggaaatag tttgacgata tctaccccgc
    11521 ttttcagcgc tgttttgatc tcggtaatag tcatacatcc tggtaagtaa ggaatttgat
    11581 acagattgca catctctgcc gtttcttgat caaaggaagg gctaacaata tactctgcgc
    11641 cagctaagat agccaagcga gctgtgacag cgtctaatac tgtgcctgcg ccaatcacta
    11701 tttctggttg gtctttgtag aatgacaaaa gttctttgat tacttgatct gcttgaggga
    11761 ctgtaaaagt caactcaata ccagttagac cacctttgat gattgcgtgg caagcattca
    11821 aagcttcttc tttgcttttt ccgcgtacca ccgcaatcac tccggcgttt ttcaatcgag
    11881 ataaaatatc aatttttttc atcttctcca cctatttcct atttagaaat tttattttat
    11941 aacttggaaa tctataccca tattatacgg cagaaaagaa aaattgcaaa cgcttttttc
    12001 attatttttg aattctttgc tcgatttcgc tttttgtttg cttgagggca ctgattattt
    12061 gttcttttgt ctcatctgtt aaacgatatt tcggcatact tacactaaat gcaccaatga
    12121 tttgttcgtt tttcacgatt gaggctccta tacaaaaaat atcacgttcg atttcttcat
    12181 cgtcaaatgc gatgtgggtt tcttttaccg ttttgatttc ctttttcaac cgtaatgcat
    12241 tggtgatcgt atattctgtg taagggatca acggatgctt ttcaagataa tcagtgtact
    12301 gctcttcact gaattctgct aatacggctt tgcccatcgc agaattgtat aatggtcggg
    12361 tgattccgat ttttgaagac atgcggatcg tttgatgttt tggatctagt ttgttcacgt
    12421 acaagatctc attgttgtcc aataccccta agtggatagt ctcatcgatc ttttgctgca
    12481 gctgttctaa aaacggtaaa gtcaactcta ccaaatcgat ttgttcaatg tttttattcg
    12541 cgtagcgaat aagctttgca cctaaacgat agtttttctc tgaattttta tttacgtagc
    12601 cgatcatcaa caaagtatct agtattttca agactgtaga agaggtcatt cccacaccct
    12661 ttgcgatcgt ctgcaaagag acatcttggt tttccgctaa atagtctagt attttagagg
    12721 ctttgatcaa tacagttcca taaggctttt tattttccat ataatattct cctatttctt
    12781 tataagaaaa attatagcgg aaataccatc aatttaaaag agatatccag atataaacta
    12841 ccatttctct ttacaggcaa tttaatgttg aagatccgct tcgtttttac atcaaacttt
    12901 gatataaagc aatgatttct tctactgtcg tttctcttgg atttcctggt gtgcatacat
    12961 ctcgcaaagc atcttctgca attgctggaa tatctgcttc ttttacacct aattcagaaa
    13021 tggtttctgg gattccaacc gctttgctca accgatcgat ctcaccacaa gccgcttctc
    13081 ttacatcttc taaagacatt tcagctgctc ctttaatccc tagaacaaga gcgatctcac
    13141 ggtacttttc tccagtatat tctttgttat atttcatgac agttggcaaa agtgctgcac
    13201 acgctacgcc atgaggaatg ttgtaccatg cacttaacgg gtgagccatc ccgtgtacca
    13261 gtcctaaacc gacatttgag aagcccattc ccgcaatata ctgtcctaat gccatttttt
    13321 ctcgcccatt ttgatcgcca tctacagaag cttccaaaga atggccgatg atttcgattg
    13381 cttttaaatg cagcatatca gtcatttccc aagcgccttt tgtgataaac ccttcaatag
    13441 catgcgtcat agcatccata cctgttgatg cagccaattt tttcggcatt cccatcatca
    13501 tatcgctgtc gatgaacgcg acgattggga tatcatgagg atcaacacaa acaaattttc
    13561 ggtgatttac ttcatctgtg atgacgtagt tgatcgttac ttctgctgct gttccagaag
    13621 tcgttggtat agctaatata ggtaaacatg gatttttcgt atcagcaaca ccttctaaac
    13681 taatgacgtc tgaaaactct ggattcgtca aaataatccc aatggctttt gccgtatcga
    13741 ttggcgaacc tccaccgatt gcgatcaaac agtctgcctc tgcctcttta acaaaagcca
    13801 ctccatcttt tacattttgg atcgttggat taggtacgat cccgtcgtat aaagcataag
    13861 cgattgctgc tttatctaga agttctgtta cttttgttgc tataccgtgc tcgatcaatc
    13921 ctttgtcagt aatgacagct gctttgccga atcctcgctg ctgaaattca gggacgatcg
    13981 actcaatcgc tccttttcca aaatacgatg tttcatttaa aatcattcga ttactcattt
    14041 tctattcctc ctaatttgta ttatttttca caataaaaac gaaagaaccc caagcaaccg
    14101 gttgcccagt tgcttgttgc tacagttcaa agactggcat caattcccaa ctgactggag
    14161 aggcatctgg atttgtctcc atgatatctt ccatgtactt ccaccatttt tgattgatag
    14221 atgtatttgc cattttttcc cataactttt catcttcgat cactaagtaa ccaaataaca
    14281 aacttgtact cggatcaaga tagatcgaat agtcgatgac cccgtgttca cgaagtgctg
    14341 tacgcatttc tggccaaagt tcatcatgtc tttttttgta ttcttcttgt ttttctgggt
    14401 aaactttcat gcaaaaagct tttttcacca tgtcagacac ctgctttcgt atgaacttcc
    14461 tccaaataac ctgcttttgg agtgacacca aacgcatctg ctaaatgcca caggttttcg
    14521 tccgtgattg tttgcaagat tggtccttgc gctttgacgt acgtatagac ttctgctgcc
    14581 ttttctgctg tctcgatcaa accaaagact tcatccatgt cttttcctga cccgtagatt
    14641 ccatgttgtg gccataaaac caatcgagtt tctttcattt tttcagctgt tgcttcccca
    14701 atctcatttg ttcccggaac catccacgga atgatggaaa ttccttctgg aaaaacaact
    14761 aagcattccg tacacatttg ccatagcgta cgactaaagg aacgttcgtc taattcatgc
    14821 gtaaaactca tcgctaatag atgactagca tggcaatgca taacgatccg attatctggg
    14881 tctacttcta aacgggcgat atgactcatg aaatgacttg gcaactcgct tgtaggaagg
    14941 cattcgtttt ccagccccca caaaagttcc agtgttttgc catcttcagc aactcgaacg
    15001 attccaagat tctcggcagg ttcgctcgct acatttttaa aatatcttcc tgaaccagtt
    15061 acaataaaat agcatcctgc taaacggctg gcatcaaaaa tcatcgagat gcggcggatc
    15121 actttttcag gatccaagaa cggtgtgatc tcttcttctt ttagtaaata agaaatattc
    15181 ccgccatttc tttcatccca tcccaagcga taaagattcg acgtcgtttc gatcatttct
    15241 ttcacataag gcgcttgtaa aatatctatt tttttcaata tagccacact cctattttct
    15301 taattccaac acgtcttttt gatattcctg gacttcttcc agccaatcca aaccaactgg
    15361 aacttgattt ttgtaacaga aataattcca tacatcggca tacgggaaat ctttcaattc
    15421 ttccgtataa gccattcttg aagtaaagtc aaaagccagc tccatttctt tcaatcgctc
    15481 aactggttcc agcatagctt tcagcagtgc tttttgtgta tttctcgtac cgatgaccca
    15541 agcagcgata cggttgatcg ttgcatcaaa gaaatccaga ccgatatttg ttttttccaa
    15601 caattcgtta cggactaatt ctttagcgat ttcctgcaat tcatcatcca tcaaaaccac
    15661 atggtctgaa tcccaacgca ctggacggga aacatgcaac atgattcctt tgctgaataa
    15721 agctaatgaa gataatttat tggaaatcac ttctgttgga tgaaaatgtc cagcatctag
    15781 acaaacaagt ttgtcacgag tcaacccgta acccatataa aattcatggg aaccgactgt
    15841 ataagcttca gcgcctaaac caaatagttt gctttctact gcatcttgta cataccgttc
    15901 atctaactct tctgtaaaaa tttcatcgag tgattccatt aaacgttccc gtggggtaaa
    15961 acgatcaatc ggattatctt tcatcccatc aggtacccaa aagttattga tacttgtttg
    16021 cccagtttct ttcccaaagt actccgcgat tttgcggctt cttttcccgt gttcgatcca
    16081 gtattcacga acttctttat ctggatgagc caatgtaaag ccgtctttca tcattggatg
    16141 cgaaaagaaa gtcggattga agtcaagacc gatttcttct ttttttgccc actctaccca
    16201 tttttcaaaa tgtttcggct caatttcatt tagatcgaca gattcttctg tatccaaata
    16261 gatagcatga aggttcaatt tgtgtttgcc cggtatcaac gaataagcct tctccagatc
    16321 ttgtctaagc tcttcaggtg ttcgtgcggc tcctggaaaa ttccctgtcg ccatgattcc
    16381 accggtcaat tcgccatccg gtgtcaagaa acctttgaca tcatctcctt gccagcaatg
    16441 catagaaatt ttgatgtcct gtatcttttc tagtacagca tctgtatcta caccgatagt
    16501 tgcatatttt tcttttgctt gttcgtaatt tctttcaatc gtgttcatac gattactcct
    16561 ttgattttat tcacgtacca ttccatcgtg ttttgcttat tttcatctgg gtaaaaagtt
    16621 tcacatggaa acgactgttt gattacattt cttgcttgtt ctatattcga aaactgacct
    16681 gtactgagca tttgcatggc aacattcccg attgctgtgg cttcactagg tcctgcttct
    16741 acaatgattc cggcataatc tgctgtcaat tgattcagta gacgattatt cgatccgccc
    16801 cctacgatgt gcagcttttc caatttcgtt ccagttaagt gttctacttt gatcagctct
    16861 tgtgcgtaac aaagagcaag attatcatac acacaacgag cgatagctcc cgatgagaca
    16921 gggactggct gctctgtttc aaaacagtat tgctgaatct cttctatcat atttggggga
    16981 ttcaaaaatc gagggtcatt gatttccatc acttgctgca gcggtttttc ttttgcagca
    17041 agctctgcca attccgcata actgtactcg tactcttgca ttcgggcaac ttcctggatc
    17101 aaccacatcc ccatgatgtt tttcaaaaaa cgcaccgtgt tatgggcacc ccattcattc
    17161 gtataatttt cagaaaaagc attgcgagaa acattggcga tctttgtttc aatccctaaa
    17221 agcgaccatg tcccactgct gatataaccc caacgttctc cttgacctgg cgttcctaat
    17281 acagctgacg ccgtatcatg acttgctact gtgataaagg tcacatctgg caaatcatat
    17341 tttgaaaatt gttctttttg cagatctcct agtatcgttc ccggctctgt caatggtgga
    17401 aataattcct tcgagacacc gattttttca agaagttcta tttcccattc ttttgtccct
    17461 gcattcagca gctgcatagt agatgcatta gttttctccg tcaccatgtt accagtaaag
    17521 acatacccta aataatcagg tatcagtaac aatttatgtg cttttgccaa attttctttt
    17581 tcttcagcaa acaattggaa caaggtattg aaaggctgga tctgaatccc tgtctgctca
    17641 tacaacttat ctagagaata gatttcagaa aactgttcta ctgctccatt ggtacgcttg
    17701 tcccgataag caatcggatc atttatccga tccccttgtt catcaatcag acagtaatcg
    17761 actgcccatg tatcgatccc cgcaaaaagg cgatcaatcc ctgtctgctt taatttttca
    17821 aatccaatca aaatctcttt gataagataa tcgatcttcc agcgatcgtg accgtctttg
    17881 ttccgaaagc cgtttttgaa ccggtgtact tcttccaaga tcaaagaacc ttttgcatcc
    17941 ctcgtacttt tcattagtcg tccgcttgat gcaccgatat cgatggcaac aaatgttgga
    18001 ttcatttctc cacctctttt actttattgt tgattagcac tttattaata aagcgttatc
    18061 atttattgac aatgctatca taatacagaa tcttatattt tgcttcacac tattttggta
    18121 tataattgca ttatcttgac aagttgggag tgtttacatg gattactggg aaaaagccaa
    18181 ccactcatgg tcggaagact caaaaagagt gatcttgacg cctacacaaa gaagcaggaa
    18241 tttatttttt tatatccaag aaatcggtta cttcaaagca ccaatccctt actatactga
    18301 acgagcacat ttaccttctt acttgataaa gtttactttg tctggcaaag gttcgcttac
    18361 atataatgat aatcagcaac agctaaaagc tggagactta ttttttattg actgccaaga
    18421 atatcagcgt tatcaaacga tcagcgaaga accatgggaa atggattgga tccatttgaa
    18481 tggtcagaac gtccaatcat tttatcagga gttcacaaaa gacggccata acaccttcca
    18541 ttcaacagga gatccatcag aaaacaagat ccaccatttg atcacagaat tgatccacat
    18601 gcaacaaggc acgaatgccc gtaccgattt ccagtcctct gtagctatcc acgagttgct
    18661 gaatgaactg attttgcaaa agtatcagct agatttcgaa gaagaggata ttcccgccta
    18721 cgtttacaaa ctgaagcaat acttagacga acatttcaaa gaaacgatta cacttgatca
    18781 attagaacaa caattcttat tgaataaata tcagctgagc aaagattttt ccaaatttat
    18841 cggttctcca ccaatcgatt atttgatcag caaaaaactt tcttacgcta aagaccttct
    18901 tcggtataca gaatttacgg ttcgaatgat ttccgaagaa atagggattg agaattttgc
    18961 ttactttagc cgcctgttca aagcaaaaac aggactatcg ccgagagact ttcgtaaatt
    19021 cagctgattc atataatatg tacgtcattt tttcaaaata ttttgaaata tatagataaa
    19081 aataaaataa ccgcagtttc tgcttctatg taaacaacag cagctactgc ggttattttt
    19141 tagtgatcat tctcattgtt catcttcaaa atgttcataa tgaaccaact gtttctgtgt
    19201 atcacgtttc aaaaattctt taggcgttat gccgaactct ctcttgaatg ctttcagaaa
    19261 atgcggataa gaagaaaaac cacttttact atatacagca ctagagctgc ggtattctcg
    19321 caacaagtgc ttggcataga tcagcttttt cttcattaca tactgatgaa acgtaaatcc
    19381 agtgtacttt ttaaattctc gtgtgatatg gtatttgctg acaaaaaact gcttttccat
    19441 ttcctctaaa gaaagcggat gagataaatt ttttgagata tattgtaaca tttgttccac
    19501 acgctcatta ggaactacag cattctgttc ttgcgcactc tcttcttttt gtacaacttg
    19561 attgagaaaa atcaagtatt ctaccagagc ttgttcatat tttatgtctt caccgtaaat
    19621 ttttttatca ggaggatggt cgataaatga taatttttgc ttcaatactt cctgttctat
    19681 ttgcaataca cgactcctct tctctcccat tggttgaaaa caagccgata gattagacca
    19741 tggcgtggaa cgactttcta gaaaagaagg ggtgacaaag atatagacac gctcgaatac
    19801 atctgaagat tggcggacga tacgatgcaa atcattggaa tggatgagca gcacacttcc
    19861 tgctttgatc gtaaattgtc gtccgttcaa ataaaaagtc gcttctccct ttaatgtagc
    19921 atggatctca tagaaatcat gatgatgata gatcgtttta ttttggctga cgacatcttt
    19981 gacatgatag atttcatact gatcagaaac catcgtattg acttgttcac cgctgatttt
    20041 cttttggagc atgtgccgca cctccttttg ttcacttatg cacaatttta ttataccacg
    20101 ttttttgtaa tcaaaaagag ctgcttttat atactcttat aaaaaagaag agacgtggca
    20161 tcttctagcc ccgccccctc cattctaaat attagttaac caacgtcttc tttacattat
    20221 tcaagaagac ttcttccata acgaatccca tcatcttttg tttcttcaag aaccacatac
    20281 aggtacggac gattcttcat gatgatttct ttgatttttg tgtactcgat cacaccgtct
    20341 cccaaattta ccggcacgat tttttcctgt tcgacacgaa aatctttcaa atgaaccgca
    20401 catatctttt caccaaaacg ttcaaaagct tcttctacca attggacttg ttcttgataa
    20461 tcactacttg tgatcaaatt ggtcggatct aaaatgattc ctagatagtc cgaatctact
    20521 gcttggatca gctgctctac ccttgctaaa gaatacaccg gatgattcaa acctggttct
    20581 atccccacca tcatttggta tttttctcca gcttttacca agcgacggat aacggacaca
    20641 gcttcagcaa atgcttcatc tgtgaaattt tcttccgtat attggatttc cggcaaaaca
    20701 catccagttt ccgaagcaac catcgacgca ccaaaggaag aagcatagcg aagatactgc
    20761 tcgaactttg tcagcaactc ttgtcgtatc gttaaatctg gatgaatcat attgatatag
    20821 caacttaaaa ttcctacggt cacttcttgc ttttctaaaa ctcgtttgac ataatttccc
    20881 atcccagaat tgatctcatt tgcgccagaa ggtatttcag gaaacgaaag acctaaggcg
    20941 agctgcagtg tatgaatccc ttgttctttt gttttttctg caagatcttc tatcgtttgg
    21001 acttcggaaa gatcatgtcc tctaattgta atgttcaaca taactattct cctaactcag
    21061 catattgggt agccacatga cgatttgcgg gaagtaagta atcaaaaaca agacgactaa
    21121 gataacccca taaaaaggaa gaagcggttt cagcattgcc tctgctttta cgcctcctac
    21181 acttgcaccg acatatagtc ctgtacctac tggcggcgtg atggagccta cacacagatt
    21241 catgattgaa aacaacccgt aatgtacagg atcaacccct acacttgtga cgattggtaa
    21301 aaatattggt gtaaaaatca agatagcagg cgccacatcc ataaacatac ccaccagtaa
    21361 taacacaaga ttgactaata acagaataat aaatttatta tccgttacgc ccataatcaa
    21421 cgaactaatt gcctgaggaa taccggtaaa cgccatggcg aaagacatca tggaagaagt
    21481 agccaataag aacatgatcg tccctgacat tgctactgat tgaaacaaca tattaggtat
    21541 atcttttagt tttaccgttt tatagtaaaa catagcaatc aacagggagt aaatgacagc
    21601 tatcgctgaa gcttctactg ctgtaaatat ccccgtcaag ataccaccga taatgatgac
    21661 aatcaataga aggctaggaa tcgcctcttt tattattttt ccagcatgag ccatctgtcc
    21721 tttttcgaca acagggtatt tattcttttt agcaacgatg aatgctacta gcatgattcc
    21781 tagcgcccac aaagcgccta cgagataacc tcccataaac aaagcagaga tcgaagcacc
    21841 accagcaaca agtgaataca tgataaaagc agtacttgga ggaatgacca tacctgtcgg
    21901 agctgacgcg atattgaccg ctgtcgcaaa cttccggtca tatccttctt ccacttgctg
    21961 agggatcaac actccgccaa tcgcggtaga agcggcaatg gccgagctag agatggaacc
    22021 aaataaagca ttccctaaca cattcgtatg agccaaagcc cccggcacgc gcccgacgaa
    22081 caacatcgca aaatccacta gtttcttagc tatccctcca ttattcatga taatgcccga
    22141 gagaacgaaa aacggaatag cgatcagcga aaaactattg atacttccca ccattttttg
    22201 agctgaacta aaagcagaaa catcaaacgg tataactaac aataaagtca tcatcgatga
    22261 gaatgcaata cttatagcaa tcggcatccc taatacaagc aacacaacaa atacaaaaaa
    22321 taacactaat ccggcttcta ttgccatttt attcctccat ttttatattt tttcttgaat
    22381 tgctggcttg ttgcttaatg tatcgattag attgatcagg ctatagataa tgatgagtac
    22441 ccccgataca ggtactgaca gataaacata tcccatagga atacttaaag aaggcgagat
    22501 ctgccccatt gtcaatgtga cagcattcca tccgccatac accatgatca tcgcagcaaa
    22561 taccagaaat aaactttgaa caacaacttc taaaatttta ctttttttgc cagttaattt
    22621 ttcacttaaa accgttacgg ctaaatgtgc tttttgtccc acaacatacg cggaagacaa
    22681 catggcaaac cagacaagac cgaatcgaac aagctcttcc gtgaccgtat ttggatttcc
    22741 taaaaccgtt ctggaaaata cttgccataa aacaacagcg accatcccaa caagcaaaac
    22801 agctcccagg atctctaata ccttgtttaa aatcattttc atcacagcta cgctccttgc
    22861 tcttggatct tttgataaag gtctttgtag gtaggatcgt ttttgaatat atcatgaagt
    22921 ggggcaactg cctctcgaaa cggtgtatta tctacatcat tgaattggac tccatgttct
    22981 tcttgtgcta aagctttttc ttcttcaatt gcttttgcaa aagcttcaat ttcaaaaacc
    23041 gttgattctt tcgctgcttc ttcaatggct ttttcctgct catcagttag ctgttctttg
    23101 atggcatcat tcatgatgat gatatctggc acacgtgtat gctcatcata ggaatagtat
    23161 ttagccacac caccatgtcc agctgtatac aagacaaatt cattgttttc tgatccgttg
    23221 atcaaattgg attgaagaga cgtgtacact tcatcacttc ccataggtac gggcgaaccg
    23281 cctaaaagct ctaccatttt gatagcggtt tcgctttgca tcacacggat cttctttccc
    23341 ttcaaatctt ccggcgtatg gatcggaccg tctaccatat aaaagctgcg ctgaccagaa
    23401 tcataatagg tcaatccggt aaatcctaaa tttttggtcg attggtagat tggctccatt
    23461 atttcttcat tatccatcac cttaaagaaa tgttcttcat cattgaatag ataaggaact
    23521 gaaaaaatcg aataatcttt agagaaactt tccaacgctg aggcacttac ttttgtgaaa
    23581 tctactgctc ctgactgtgt caattcgatc agttcacgtt ctccacccag ttgtccatct
    23641 gggaaatact ggatctgtac ggaaccatct gttttttctt ttactaaacg cccaaattca
    23701 ttcattgcat caattactgg ctggctgttg cttgcataag caaagcgcaa ggtaactatc
    23761 tcatttacat ctgcatctgc gacagttgcc acactagaat tatttccgca gccgcttaga
    23821 cctagcagac ctaggctaag cgaacttgct gcaaacagca gtgatgtttt tttcatatgg
    23881 gtttcctttc ttttaacaat aagagacgag caattctgtc aaacaaagca ccgttaacga
    23941 ctgtccataa ggcattgctg tgattccgat attccgataa aaatctaaac tgtcccccat
    24001 tcctgtcccg attgaaacgt tttgaacttc tcctttttca tcgatttgtt caagcaatcc
    24061 ttggatagca cgataagcga cttctttata ttcttgtggt aaataacgtt tatggaccgc
    24121 tttcaaaata ccgtaagcaa atcctgcagt tgcagaagat tctacgtaag aagaaggatc
    24181 atccaacaac gtatgccata aaccgctttc agactgataa gtttttaatg cacgcacttg
    24241 tgcttcaagt gtttcgatca ataatttgcg caatgcatct tctttcgata attctaagat
    24301 ttcaatgatc tctggaatag caatcgtcaa ccagcagttt ccacgagccc aaaatgcttc
    24361 ggcatagtta tgatttcctt caaacgtcca gccgtgatac cacaatcctg ttcgtttatc
    24421 catcaagtat ttgatatgga tcataaactg gtaacgagct tcttctaaat attctgggcg
    24481 attcaacagt ttcccgattt ttgccaaagg aagcactgtc atcatcaatg tatcgtccca
    24541 caactgattc ttattttccg gtccatatgt ggcatgctgt aacccgtctt catttgtccg
    24601 aggcatgtca ttcatgaccc attctgccca ttgttctaaa taaggcaaat agcgtgaatc
    24661 tttcgtgtct tcatacaagt aagccatagt caatagcggt gccattgtat tgacgttttt
    24721 gggcggagcg ccttcttcca tacgatcctc aaaccattca ttgaccactt gataagcgcg
    24781 aggatttttt gtcatctggt aatttttata aataccatat agaccgacac cttgtggcca
    24841 attccacact gtccagcttt tgtcatctac tttcagtcca tcaaagtcca acaaaaattc
    24901 accattatcg tctttgatcg ttgtcaggtt ttcaacgagc aggtcaattt tttctttcac
    24961 taattcttta ggtacaaaaa tctcttcttt aataatcatc tttacgccac ctttttaaac
    25021 gctttcattt tatatcctta atatagcagt aataccgtcc actataaata tcgctttttg
    25081 aacaaaaact gtctaaaatt gacctaaatg ttaaataaca aaagtaactt tcagttactt
    25141 ggaaattaaa aaaagaactt ggagaaacaa cgttttccaa gttctttttc atctattttc
    25201 ttatcttttt tctacgcttt cttttgctta tccgttgtaa gcatcaatga tgatctgttt
    25261 caattcagag atcaatggtt ctttcggatt agctgtcgta cattggtctt cgtaagctaa
    25321 ttcagccata cggtctacgg ttgtatctag tgtttcttgt gtgactcctt gagcttttag
    25381 actcatgttg atgccgacag attttcctaa ttcataaaca gccgttgcta aagcttcgac
    25441 taattcctct gttgtttcgc ctttcagacc taagaatttt gcgatatctg cgtaatctgt
    25501 atctgcacgg aaatagtcat atttagggaa catcgcatgt ttttgtggat cttttgcatt
    25561 atagcggata atatgcggta aaagaatcgc atttgtccgt ccatgaggga tcccgtattc
    25621 cccgccgatt ttatgtgcga ctgagtgaca aatacctagg aatgcgttag caaacgccat
    25681 accagccatt gtagaagcgt tgtgcatttt ttcacgtgat tccatatcag gtgtttttac
    25741 tgatttttct aaatagtcaa agactaactt gattgcttgt aggctcaatc cacgtgtata
    25801 atctgaagcc atgactgaaa cataggattc gattgcatgt gttaacacat ccatcccggt
    25861 atctgctgtg acagatgctg gaactgacat gacgaattga ggatcgacga ttgcgacatc
    25921 tggcgtcaat gcataatctg ctaacggata tttcacatgt gtttcactgt cagtgatgac
    25981 cgcaaatggc gttacttcag aaccagtccc tgatgttgtc gggatacata cgaattgtgt
    26041 tttttctggt ttgtcgatct tatatgtccg tttacggata tccaagaatt tttgttttgc
    26101 accaaagaaa gatgtgtctg ggtgttcata gaacatccac attcccttag ctgcgtccat
    26161 tgcagaaccg cctccaagag cgattactgt atctggcttg aagtctgcca tcagtttcgt
    26221 tcctgcgtat acggtatttg tcgatgggtt cggttcaaca tctgagaaga cttcgatctt
    26281 cacatcattt ttacgtcgtg ccaatacttc acgaactgta tcgcagtaac cgaattggac
    26341 cataccaggg tcacaaacga tcatgacacg ttcaacgttt tccattttct ctaagtacaa
    26401 tagagagttc ttttcaaaga agatttttgg cggtaatttg aaccattgca tgttatttct
    26461 ccgtttcgct acagttttga cattgattaa gtttacagca gagacgttac gagaaacaga
    26521 gttcttacca taagaaccgc atcccaaagt caatgacgga atcatttcgt tatagatatc
    26581 accgatgccg ccttctgcag atggtgtatt cactaaaata cgacaagctt tcattcgaag
    26641 accgaatttg acatgcaact cttcgttttc tgaatggata actgctgtat gtcctaatcc
    26701 tccaagattc aacatacctt cacacaaatc aaacgcatgg tccgtgttgt tagctttgat
    26761 catggcaaga actggagaaa gtttttctct tgatagcgga taatctgggc ctacgccttc
    26821 caattcagca atcagcattt tcgttccttt tggtacactg attcctgcca attttgcgat
    26881 ttccatcgca gaatgtccaa cgattgatgg attgaccgca tattttcctt cgttcatcac
    26941 tgcatcttct aatttttgca attcatctgg ttttacgata taaacttggt gtgcttggaa
    27001 ttctgctttt actgctgcat agatttcttt atccacgatc acagcttgtt cagaagcaca
    27061 aatcattcca ttatcaaatg ttttcgaaac gatcaaatcg ttcactgcac gtttaatttt
    27121 tgctgttttt tcaatataag ctggtacgtt acctggacct acacctaaag ctggttttcc
    27181 tgtagagtaa gcagacttga ccattccagc tccacctgtc gctaagacga tcgcgatacc
    27241 tggatggttc attagcatag aagtcgcttc gatcgaagga tgttcgatcc attggataca
    27301 attttcagga gctccggcag cgatagcagc atcacgtacc actcgtgctg cttctgcaga
    27361 agatttttgg gcacttggat ggaaagcaaa aacgattgga tttcttgttt tcaacgcgat
    27421 cattgatttg aaaatcgttg tggaagtcgg attcgttgtt ggagttactc cgcaaaccac
    27481 accgactggt tcagcaattt caatcaaccc tttttggaca tcttcattga tcacaccaac
    27541 tgttttatca tgtttgatat tatgccaaat atattcggac gcatacatat ttttgattgc
    27601 tttatcttcg taaacaccgc ggcctgtttc ctctacagcc atcttcgcta atggcatatg
    27661 ttgatccaaa gctgccattg ccatttcatg cacgatatgg tctacttttt cttgatcaaa
    27721 gctttccatt tctttcaatg ctgcatttgc tttttctgcc aaatcatcaa tcattgcttg
    27781 tacgtcaatt gtcttcgttt tttctttttc tattacttta gccatcatta tcctcctaat
    27841 gggtcatggt tatttgttaa ataattcaca tgtatatcat acaatattta tttttctatg
    27901 taaacacttt tttgtgattt ttttcacagt tatttttaaa tataaaattg c
//
LOCUS       DARBEC010000030        26606 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.030, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000030 DARBEC010000000
VERSION     DARBEC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.030"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1152
                     /locus_tag="SCL80_001211"
     CDS             <1..1152
                     /locus_tag="SCL80_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEG1107093.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="SCL80_001212"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="SCL80_001212"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEG1107094.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="SCL80_001213"
     CDS             2728..3003
                     /locus_tag="SCL80_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEG1107095.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="SCL80_001214"
     CDS             3009..3776
                     /locus_tag="SCL80_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEG1107096.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="SCL80_001215"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="SCL80_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEG1107097.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="SCL80_001216"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="SCL80_001216"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEG1107098.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="SCL80_001217"
     CDS             7920..8222
                     /locus_tag="SCL80_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEG1107099.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="SCL80_001218"
     CDS             8225..9388
                     /locus_tag="SCL80_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1107100.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="SCL80_001219"
     CDS             9414..12842
                     /locus_tag="SCL80_001219"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEG1107101.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="SCL80_001220"
     CDS             12863..13987
                     /locus_tag="SCL80_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107102.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="SCL80_001221"
     CDS             13977..14414
                     /locus_tag="SCL80_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1107103.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="SCL80_001222"
     CDS             14398..14736
                     /locus_tag="SCL80_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEG1107104.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="SCL80_001223"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="SCL80_001223"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEG1107105.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="SCL80_001224"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="SCL80_001224"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEG1107106.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="SCL80_001225"
     CDS             15893..16936
                     /locus_tag="SCL80_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEG1107107.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="SCL80_001226"
     CDS             17095..17823
                     /locus_tag="SCL80_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEG1107108.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="SCL80_001227"
     CDS             17939..18184
                     /locus_tag="SCL80_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1107109.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="SCL80_001228"
     CDS             18181..18399
                     /locus_tag="SCL80_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEG1107110.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="SCL80_001229"
     CDS             18535..19074
                     /locus_tag="SCL80_001229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEG1107111.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="SCL80_001230"
     CDS             19067..21340
                     /locus_tag="SCL80_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEG1107112.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="SCL80_001231"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="SCL80_001231"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEG1107113.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="SCL80_001232"
     CDS             complement(22513..22767)
                     /locus_tag="SCL80_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEG1107114.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="SCL80_001233"
     CDS             complement(23167..23436)
                     /locus_tag="SCL80_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107115.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="SCL80_001234"
     CDS             complement(23906..24361)
                     /locus_tag="SCL80_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEG1107116.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26427)
                     /locus_tag="SCL80_001235"
     CDS             complement(24499..26427)
                     /locus_tag="SCL80_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HEG1107117.1"
                     /translation="MTNQKKAFAATIGCLLFMIFGFIFDRFSLTITPLFYISAIIIGG
                     YKQTSEGLKELWNDRTLNVDLLMALAAIGACLIGNYFEGAMLTFIFCLSGALEEYTTN
                     KSQKEITALMNLQPQKAQLLKENGQMQEVDVSQLKVDNLVFVAKGAAVPIDGFLESTQ
                     ATIDESALSGESVPVEKYLGDEMYAGTLNQGNPITIRVTKTSSETVFAKIIQLVEEAQ
                     NTPTQTASFIERIENNYVKLILLAVPLMILLPHFFLGWSWDESFYRGMVLLVVASPCA
                     LVASATPASLAALSNSAKNGILIKGGIHLEKLSELKAIAFDKTGTLTKGKPVVTDSFF
                     FDEKDLAQQLLVAMEQKTTHPLAHAILQYFDCTIPEEIQYLPIEEITGFGLQTTYLGA
                     QWKVGKHAYDPETMIISKEIAEMIERLENQGKTVIYLSKDQQLIAVLGLLDIPKANTQ
                     QVISYFKSQNIHTSMITGDHSGTAKAIAEQVGIEHYYASCTPEEKTQLVKKENELYQV
                     NAMVGDGVNDAPALAAASLGVAMGQGTDAAMDIADIVLMKSDLNKFVYSHRLSLKMRR
                     IIKQNIIFSILVILLLIASNFLQFLNLPLGVVGHEGSTILVILNGLRLLRPLPDLSDS
                     SKKCKSCPLYKARLSSVK"
BASE COUNT         8861 a         4610 c         5615 g         7520 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggttatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttatttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccaccttt agttgagaaa catctacttc
    26041 ttgcatttgt ccattttctt ttaataattg ggctttttgt ggttgaagat tcatcaaagc
    26101 agtaatttct ttttgacttt tattagttgt gtattcctct aatgcaccgc ttaaacaaaa
    26161 gatgaatgtc aacattgctc cttcaaagta attaccgatc aaacaggcac caatagcagc
    26221 taaagccatc aataaatcca cattcagtgt ccgatcattc cacaattctt tcaagccttc
    26281 cgatgtttgt ttatatccac cgatgataat ggcactgata taaaaaagtg gggtgatggt
    26341 aagagagaat cggtcgaaga taaaaccgaa gatcataaat aaaaggcagc cgattgttgc
    26401 tgcaaaagct tttttctgat tagtcatcgt tattagacct ccttgtcaaa acaaggatag
    26461 cataataaaa atgtaattga taataataac agctaaatga gaacgataat ggttatcatt
    26521 tggtgattaa gcttgatgtt gtttttatta tttataatga ttattattaa agaaaataaa
    26581 aaaccagaac atttctcaaa taagag
//
LOCUS       DARBEC010000031        26398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.031, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000031 DARBEC010000000
VERSION     DARBEC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 26398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.031"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            53..1819
                     /locus_tag="SCL80_001236"
     CDS             53..1819
                     /locus_tag="SCL80_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003056564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-hexosaminidase"
                     /protein_id="HEG1107118.1"
                     /translation="MTIKVDLRKKPYHLDEDAISWIEETIDAMTLEEKIGQLFVNMGS
                     SRDKNYLSSMVNQYHIGAVRYNPGTADEVYDQNYILQTESKIPLLIAANTEAGGNGAC
                     TDGTEVGVEVKIGATNDAKYAYELGRVSGIEASAIGCNWSFAPIVDINRNWRNPIISS
                     RSFGQSPDKVLELSLAYMKGIQESGILPAAKHFPGDGIDERDQHLSFSINSLSTEEWD
                     NSFGKVYSGLIEAGLPSIMAGHIHMPAYSKHFNLDLKDEDLLPATLSKELITDLLRGK
                     LGFNGLVVTDASHMLGMTGAMKRSELLPTSIAAGCDLFLFFNDPDEDFNYMMEGYYKG
                     IISEERLRDALRRILGLKAKLGLHKKEKNKILLPKQEAMEKIGLIENKSIFKEVADQA
                     ITLVKDKQDIWPIVPEKYKRILLVDVHGVKGGFGEVVAGNRPQAIDLLKQILSAKGFE
                     VEKYISPEEELKGLSQEEKIKNIAGIYAAKRPIKDLTEKYDLIINIANVNPGTVQRIV
                     WPASKGTPDIPFYIHEIPTIFVSVQCPFHLADVPQVKTYINTYDGKQETLEILVDKLM
                     GLSEFKGISPIDAYCGLKDTFI"
     gene            1847..3655
                     /gene="uidA"
                     /locus_tag="SCL80_001237"
     CDS             1847..3655
                     /gene="uidA"
                     /locus_tag="SCL80_001237"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017767467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HEG1107119.1"
                     /translation="MLYPIMTETRQVIDLSGLWRFKLDTNDDLQEELALEPMVEKESY
                     PISVPSSYNDLFETENIRNHVGWVWYEKEVILPKKLLEERLVLRFGSATHEAKVFLNG
                     KLINQHKGGFTPFESEINQFIKDGSNRITVAVNNVIDDSTLPVGILKETIQEDGDIKK
                     ENLVNFDFFNYAGINRPVKLYTTPKDYIKDVTINTKIIDSDANVEFNIDTEWTGEILI
                     AIYDEEAQLIAEAKGKEGVIQLKNPTLWEPLNAYLYSMKVSLINENKVIDTYTEEFGI
                     RSIQVSNGKFLINNKPFYFKGFGKHEDTYINGRGLNEAVNIKDFNLMKWIGANSFRTS
                     HYPYSEEIMRLADREGIVVIDEVPAVGLHLNFMATLLDDNVEKHDTWKEIRTENAHKM
                     AIKELIERDKNHPSVVMWSIANEPDSDSEGAKEYFEPLVELTKELDPQKRPVTIVTYL
                     KSTPDICKVGDIVDVLCLNRYYGWYVSGGDLKHAKYLLKKELDGWMERCPDTPIMFTE
                     YGADTVAGLHDTVPTMFTEEYQVGYYEANHEVIDKCDNFVGEQVWNFADFETSQGILR
                     VQGNKKGIFTRDRKPKMIAHYLKKRWSSIPNFSYKK"
     gene            3827..4228
                     /locus_tag="SCL80_001238"
     CDS             3827..4228
                     /locus_tag="SCL80_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010818872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1107120.1"
                     /translation="MQQNYNEKSIQHQFDAYCKKVLRNETKSIRKRNINITDNEEPLT
                     NSNEGKYSQTHFDEQDVYFLFGMEILISDQKLSAAIDHLSEIRRKVILLYYFAGFNDT
                     EIGKILNMSTSGIWYQRKKAVEQLKMGYDLW"
     gene            4222..4467
                     /locus_tag="SCL80_001239"
     CDS             4222..4467
                     /locus_tag="SCL80_001239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107121.1"
                     /translation="MVDNPKKRQYPLVPLSIIQSALEEDPISIKMILKHYHSYIAKLC
                     LTNGFNEAGQFITYVDEYMLRQLEIKLIEAILKFKIN"
     gene            complement(4791..5012)
                     /locus_tag="SCL80_001240"
     CDS             complement(4791..5012)
                     /locus_tag="SCL80_001240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107122.1"
                     /translation="MRNEIANITIIRETLQNHTANEISKHTGLNLSTIKKLKSGERSI
                     EKLNLHDAICLTEFGLKNNRKNVEINIWK"
     gene            complement(5174..5246)
                     /locus_tag="SCL80_001241"
     tRNA            complement(5174..5246)
                     /locus_tag="SCL80_001241"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5211..5213),aa:Lys,seq:ctt)
     gene            5322..5552
                     /locus_tag="SCL80_001242"
     CDS             5322..5552
                     /locus_tag="SCL80_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107123.1"
                     /translation="MDFQDYEKIFESYPSILKITDLTKMLNIGKNKAYNLLKDRSIEN
                     IKIGARYKIPKKAVIEYILKIQDEGKNHAIIK"
     gene            5596..6765
                     /locus_tag="SCL80_001243"
     CDS             5596..6765
                     /locus_tag="SCL80_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1107124.1"
                     /translation="MKLRANIIEKRGTLQVVVSYKDSNGKIRQKWKDTKLKKKGNKKR
                     AEKIRDEFLLELESQLAMECECANPNLLFYDYLLNYLEVSKKQVAFNTYVGYKHYVHN
                     RIYKFFYPKKIKLNRLKPYHLQEFYQYMLNDNCTANTVIHYHAFIRKALQEAVITELI
                     STNVADKVRKPKKKQFVSQVYNQQEILKLLDILSEEKLYLVVLLTAFYGLRRSEVLGL
                     KWSAIDFLNKKIMINHVIIENPENMSQLIKKDQTKNSSSYRTLPLVETIERALVNQAK
                     WQQDNRILLGDDYILEDNEYVFTMEDGRLMKPQYVTHRLSKLIKKNGLKKIRFHDLRH
                     SNASIMLDSGQNMKSIQEWLGHASYSTTANLYTHLTAGVKDRAAEALENAFGFKE"
     gene            7339..7908
                     /locus_tag="SCL80_001244"
     CDS             7339..7908
                     /locus_tag="SCL80_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEG1107125.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            7895..8755
                     /locus_tag="SCL80_001245"
     CDS             7895..8755
                     /locus_tag="SCL80_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1107126.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITPEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            8945..9649
                     /locus_tag="SCL80_001246"
     CDS             8945..9649
                     /locus_tag="SCL80_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107127.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            9654..11489
                     /gene="walK"
                     /locus_tag="SCL80_001247"
     CDS             9654..11489
                     /gene="walK"
                     /locus_tag="SCL80_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEG1107128.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVILSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            11486..12802
                     /locus_tag="SCL80_001248"
     CDS             11486..12802
                     /locus_tag="SCL80_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107129.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            12803..13672
                     /locus_tag="SCL80_001249"
     CDS             12803..13672
                     /locus_tag="SCL80_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEG1107130.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            13727..14536
                     /locus_tag="SCL80_001250"
     CDS             13727..14536
                     /locus_tag="SCL80_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1107131.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(14639..15268)
                     /locus_tag="SCL80_001251"
     CDS             complement(14639..15268)
                     /locus_tag="SCL80_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEG1107132.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAASDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            15438..16730
                     /locus_tag="SCL80_001252"
     CDS             15438..16730
                     /locus_tag="SCL80_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1107133.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQHLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(16806..17468)
                     /locus_tag="SCL80_001253"
     CDS             complement(16806..17468)
                     /locus_tag="SCL80_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1107134.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            17693..17977
                     /gene="groES"
                     /locus_tag="SCL80_001254"
     CDS             17693..17977
                     /gene="groES"
                     /locus_tag="SCL80_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEG1107135.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            18028..19653
                     /gene="groL"
                     /locus_tag="SCL80_001255"
     CDS             18028..19653
                     /gene="groL"
                     /locus_tag="SCL80_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEG1107136.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDATIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
     gene            complement(19742..20890)
                     /locus_tag="SCL80_001256"
     CDS             complement(19742..20890)
                     /locus_tag="SCL80_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1107137.1"
                     /translation="MLKVWVEQTKDGQFKFIERYVDPYTEKTRKKSTTLTSNSPQAWK
                     KAQKILDKKIKEALEDYNKSDITFGELYKEWYEYYKQHVKRTSYLKVPMMMKHVSKHI
                     SDDTIVRNIDETLINKIIEDMYTFGDLSLNYTKQTKTTLSVMLNYAIDRKYIQRNPAL
                     AVKIHPKKVEEEKRRLSMNKKYLEKEEIDQILKQLYSNPRRKLHGIIAEFLYLTGLRY
                     GELLALQMKDYEDGKISINGTLDYTSVKMDNAIKTTPKNTYSQREVQLPNRAKELIES
                     VIADNILAGRPTDPDQYIFISTSGTPLALHSFNAILHKVEEELELEKSLSSHIFRHSH
                     VSLLSELGVPLKAIMERVGHSDANTTLSIYNHVTKRAKQQVIDKLNSL"
     gene            complement(20992..21576)
                     /locus_tag="SCL80_001257"
     CDS             complement(20992..21576)
                     /locus_tag="SCL80_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1107138.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(21703..22134)
                     /locus_tag="SCL80_001258"
     CDS             complement(21703..22134)
                     /locus_tag="SCL80_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1107139.1"
                     /translation="MYLKLKEMLSEYNLKVVYMEMKEPGFYYPKPRIIFLNENLYGET
                     AEAFHLSHELAHFTASHFEYSVLYDTSTTFHSKFETEADKIAILILLNIYIENELTDE
                     SQFNLEKFMEYYSIQNKLRYTCYAVCQCYFKKKYSYARQYV"
     gene            complement(22152..22472)
                     /locus_tag="SCL80_001259"
     CDS             complement(22152..22472)
                     /locus_tag="SCL80_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107140.1"
                     /translation="MNTYEIIKELAKEKGLSIRQLEMNFGYSNGYLGSWKRQTPNSIE
                     LARLADYFGVSVDYLLGREERETPKHVDLSENDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            22772..22912
                     /locus_tag="SCL80_001260"
     CDS             22772..22912
                     /locus_tag="SCL80_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107141.1"
                     /translation="MEKTVSAKIEDLKLDILKQAKVAMEHAVDKEDSAMVAAIAEILA
                     HV"
     gene            23210..23467
                     /locus_tag="SCL80_001261"
     CDS             23210..23467
                     /locus_tag="SCL80_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase"
                     /protein_id="HEG1107142.1"
                     /translation="MNKEMSQTIKVQKMIDDLTHGIDSQADKIIKELQGQKVKDAKML
                     LKTINFEMNPTKRKLADVLEEKLASAINEQELLFETDTFNS"
     gene            complement(23438..23746)
                     /locus_tag="SCL80_001262"
     CDS             complement(23438..23746)
                     /locus_tag="SCL80_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107143.1"
                     /translation="MKPEFVITMLDGEKIKIFADTLVVGYDDASNIEEKDGMFYSSQI
                     YVNTLQGDIGVSTLATSRPLVGLIGFLSKATFFSLGDDIDSDETVIYKTSAVKSIGFK
                     "
     gene            23824..24570
                     /locus_tag="SCL80_001263"
     CDS             23824..24570
                     /locus_tag="SCL80_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEG1107144.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVADVLGYSNPQKAIR
                     DHVDLEDKTQNDSFTVNGTAVVLINESGLYSLILKSKLPSAKKFKRWVTSEVLPTIRK
                     TGSYSNVPQSFAQALRLAADLEEKNQLLEQQIAEYEPKISYLDTILSSTDTVATSQIA
                     ADYGMSAIALNKLLNELGVQHKVSGQWILYRKHMNQGYTKSHTSEIPKADGGTKVVMN
                     TKWTQKGRVFIYNLLIAEGYYPQMDLEEIG"
     gene            24585..24788
                     /locus_tag="SCL80_001264"
     CDS             24585..24788
                     /locus_tag="SCL80_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107145.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            24893..25192
                     /locus_tag="SCL80_001265"
     CDS             24893..25192
                     /locus_tag="SCL80_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107146.1"
                     /translation="MKKPTLSELIEAAEKAVKPDDWYRQSLILEKFHGMSKTTLVEYC
                     KEMETIPEFAEGILRPGHSTTFIHYHTFIWFLKWKDANKYRVKSLSPSDVLKEAS"
     gene            25192..25374
                     /locus_tag="SCL80_001266"
     CDS             25192..25374
                     /locus_tag="SCL80_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107147.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEHRAKKASREGLTKI"
     gene            complement(25380..25856)
                     /locus_tag="SCL80_001267"
     CDS             complement(25380..25856)
                     /locus_tag="SCL80_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107148.1"
                     /translation="MRKKLNYILTATLTIYIILFITLSFLSSIKKIPFITLNKNSILK
                     EFNNTDYIAFLKLFSVCVLISCVIIFIVAKTCWTMLNKNQRDVENIAIFLIKITSSIS
                     IIIPMTVTFSKEQFNVAATYLSFIALFSFVLPKRNKSNFDNKELDEVRNSSPKEQK"
     gene            25909..>26398
                     /locus_tag="SCL80_001268"
     CDS             25909..>26398
                     /locus_tag="SCL80_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEG1107149.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFKKKHKHVLEAIESIKRSVENS
                     ANVEDGSNFGQMFVEGNEPDSYGRSRRVYFMNRDGFSLLAMGFTGSKAINFKLKFIEA
                     FNEMEDVIRKNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNM
                     KVM"
BASE COUNT         9106 a         4199 c         5214 g         7879 t
ORIGIN      
        1 atatatagat actttttgga aaaatcttac taataagtag aggagaaaga aaatgactat
       61 aaaagtagac ttacgaaaaa aaccatatca tttagatgaa gatgctattt cttggattga
      121 agagacgatt gatgctatga ctttagaaga aaaaataggt caattatttg ttaatatggg
      181 atctagtcgt gataaaaatt acttaagtag tatggtcaat cagtaccata ttggagctgt
      241 acgatataat ccaggaactg ctgatgaggt atatgatcaa aactatatcc tacaaacaga
      301 aagcaaaatt cctttattaa ttgctgctaa tacagaagca ggtggtaacg gagcttgtac
      361 agatggtacc gaagttggtg tagaagtaaa aattggtgca acaaatgatg cgaaatatgc
      421 ttatgaatta gggagagttt ctggaattga agcatcggct attggatgta actggagttt
      481 tgctcctatt gtagatatta atagaaattg gagaaatcct atcatttcta gtcgatcttt
      541 tggacaaagc cctgataaag ttttagaact tagtttagct tatatgaaag gtattcaaga
      601 gagtggaatt ttgcctgcag ctaaacattt ccctggtgac ggtattgacg aacgagatca
      661 acatctatct tttagtatta atagtttaag tacagaagaa tgggataatt cttttggaaa
      721 agtatactca ggcttaatag aagctggatt accttcaatt atggcaggtc atatccatat
      781 gccagcttat tcaaaacatt ttaatcttga tctgaaagat gaagatttac ttcctgcaac
      841 tttatcaaaa gaattaatca ctgacttact tagaggaaaa ctcgggttta atggattagt
      901 agttactgat gctagtcata tgttaggaat gactggggca atgaaacggt cagaattatt
      961 gcccacttct attgcggctg gatgtgatct attcctattt tttaatgatc cagatgaaga
     1021 ctttaattat atgatggaag gttattacaa aggaattatt tcagaagagc gtttacgtga
     1081 tgcattaaga agaattttag gcttaaaggc taaactaggt ttgcataaaa aagaaaagaa
     1141 taaaattttg ttaccgaaac aagaagctat ggaaaaaatt ggtttaattg aaaataaatc
     1201 aatttttaaa gaggtagctg atcaagcaat tacactagtt aaagataaac aagatatttg
     1261 gcctattgta ccagaaaaat ataaaaggat tttactggta gatgtacatg gggtaaaagg
     1321 tgggtttggt gaagtagttg ctggaaatag acctcaggcc attgatttat tgaaacagat
     1381 tttgtcagca aaaggatttg aagttgaaaa atacatttca cctgaagaag aattgaaagg
     1441 attatcgcaa gaagaaaaaa ttaaaaatat agctggaata tatgctgcta agagaccaat
     1501 caaagattta actgaaaaat atgacttgat tattaatata gctaatgtta acccaggtac
     1561 agttcaaaga attgtttggc cagcaagtaa aggtactccg gatattccat tttatattca
     1621 tgaaattcca acaatttttg tatctgtaca gtgtccgttt catttagctg atgtgcctca
     1681 agtaaaaact tatatcaata cttatgatgg aaaacaagaa actttagaaa tattagtgga
     1741 caaactaatg ggcctttctg aatttaaggg aataagtcca atagatgctt attgtgggtt
     1801 aaaggataca tttatttaat atttaaatta tataggagtg atttacatgt tatatcccat
     1861 tatgacagag acgaggcaag taattgattt aagcggtctt tggagattta agttagatac
     1921 aaatgatgac ttacaagaag aacttgcttt agaaccaatg gttgaaaaag agagttatcc
     1981 aatttcagtt ccttcttctt ataatgattt atttgaaaca gagaacatta gaaatcatgt
     2041 tggatgggtg tggtatgaaa aagaagtgat tcttcctaaa aaattattag aagaaagatt
     2101 agttcttcgt tttggatctg caacacatga agcaaaagta tttttgaatg gaaaattgat
     2161 taatcaacac aaaggagggt ttacgccatt tgaatcagaa attaatcagt ttataaaaga
     2221 tggaagtaac agaattacag tggctgtaaa caatgttatt gacgatagca ctttacctgt
     2281 agggattttg aaagaaacga tacaggaaga tggagacata aaaaaagaaa atttggttaa
     2341 ttttgacttt ttcaattatg caggcattaa tagacccgtt aaattgtata caactccgaa
     2401 agactatata aaggatgtca ctattaatac aaaaataatt gattcagatg caaatgtaga
     2461 atttaatatc gatacagaat ggaccggaga aattctaata gcaatctatg acgaagaagc
     2521 tcaacttata gcagaagcta aggggaaaga aggagtgatt caacttaaga acccaacact
     2581 ttgggagcct ctaaatgctt acttatatag tatgaaagtt agtttaatta acgaaaataa
     2641 agtgattgat acgtacacag aagaatttgg cattcgtagt attcaggttt cgaatgggaa
     2701 gtttttgatt aataataaac ctttttactt taagggattt gggaagcatg aggacacgta
     2761 tattaatggt cgtggtttga atgaagcagt aaatatcaaa gattttaatt taatgaaatg
     2821 gatcggtgct aattcattta ggacttcaca ttatccttat tcagaagaaa ttatgcgctt
     2881 agcagatcga gaaggtattg tagttattga tgaagtacct gcagtaggat tacatttgaa
     2941 tttcatggct actttattag atgacaacgt agaaaaacat gatacttgga aagaaatacg
     3001 gacagaaaat gctcacaaaa tggcgattaa ggaattgata gaacgcgaca agaaccatcc
     3061 gagtgtcgta atgtggtcta ttgcaaatga accagattcg gattctgaag gcgctaaaga
     3121 atattttgaa cctttagttg aattgactaa agaacttgac cctcagaaga gacctgtaac
     3181 aatcgttact tacttaaagt caacaccaga tatttgtaaa gtaggagata ttgtcgatgt
     3241 tttatgttta aatcgctatt atggttggta tgtttcaggt ggagacctaa aacatgcaaa
     3301 atatctgttg aaaaaagagc tagatggatg gatggaaaga tgtcccgata cgccaataat
     3361 gtttacagaa tatggagcag atacagtagc tggacttcat gatacagtac caacaatgtt
     3421 tacagaagag tatcaagtgg gttattacga agcaaatcac gaagtaatag ataaatgtga
     3481 caattttgtt ggtgaacaag tgtggaattt tgcagatttt gaaacaagtc aagggatttt
     3541 aagagtgcaa ggaaataaga aaggaatctt tactagagat agaaagccta aaatgattgc
     3601 tcactatcta aagaaacgat ggtcatcaat accaaatttc agttataaaa aataatttac
     3661 cttttataag gaatataaaa aaggagtggc tgcttaattg ccaactcctt ttttttataa
     3721 aaagattttc tgtttaaaga aggatatttt tttctagttt tttcgaaaaa aaggcgcttt
     3781 ctgattgttg tattagtgga aaggggaaaa aaggagcata tttatcatgc aacaaaatta
     3841 caatgagaaa tctatacaac atcaatttga tgcctactgt aaaaaagtat tacgaaacga
     3901 aactaagtct attcgaaaac gaaacattaa cataacagat aatgaggaac cattgacgaa
     3961 ttcaaatgaa ggtaagtact cccaaactca ttttgatgaa caagatgtct atttcttatt
     4021 tggaatggaa atattaattt cagatcaaaa gttatcggca gccattgatc atttatcgga
     4081 aataagaaga aaggttatcc tactttatta ttttgcaggt tttaatgata ctgaaatcgg
     4141 taaaatcctc aatatgagta caagtggtat ctggtatcaa cgaaagaagg cagttgaaca
     4201 attaaaaatg gggtatgact tatggtagac aaccccaaaa aacggcagta tccattagtt
     4261 cctctatcca ttatccaatc tgctctagaa gaagatccaa tttcaatcaa gatgatactc
     4321 aaacattatc atagttatat agctaagtta tgtttgacaa atggatttaa tgaggcgggg
     4381 caatttatta cctatgtaga tgagtatatg ctaagacagc tagaaattaa attgatagaa
     4441 gcgatcttaa aatttaaaat taactaacta aaaagtcccc tttccactta ttctgtttaa
     4501 aagaatcttc taatagttaa aggattagta gattctttta aacagaaaac ctattgatca
     4561 ttgaaattta aatacattct atcagtacgt taacacttca aggagcatat tttagttgcg
     4621 ccatgatttc ttactgtaaa gaaggagcga taaacattct aaaataattt ttatagtcaa
     4681 taaaaagcga tgatttgaag ttgggataat gatacttccg taaggctcgc cgtaaacttg
     4741 gagagaggtg aaattcctat gtgcttttga caaaagcagc tgattaagaa ttatttccat
     4801 atgttgattt caacattttt tctattattt ttaagaccaa attcagtgag gcaaatagca
     4861 tcatgtaaat ttagtttttc tattgaacgt tctccacttt ttagtttttt tatagtactc
     4921 aaatttaatc ctgtatgttt agagatctca tttgcagtat ggttttgtaa agtttctcgg
     4981 attattgtta tattagcaat ttcattacgc ataattatgt tcctttctta tcaaaaatat
     5041 caatgactac agtgggcttt tagtgaaaaa aaggggtact acagtctctt ttgaaaacaa
     5101 aaaaatacag tatatacgtt ttctaaatgc taagcaccaa cgtttacact gtatttaagg
     5161 gtttggcacc caatgggctg tgaggggctc gaacccgcga cccgatgatt aagagtcatc
     5221 tgctctacca actgagctaa cagcccaaat taatactttg ttattctaac acctaaaata
     5281 aaaaagtgca atacttatta tcagaattga gagtgaattt tatggatttt caagattatg
     5341 aaaaaatatt tgaaagttat ccatcgatat tgaaaattac tgatttaact aaaatgttaa
     5401 atattggaaa aaataaagct tataatttgc tgaaagatag atcaatagaa aatataaaaa
     5461 ttggagcaag gtacaaaata cctaaaaaag ctgtcattga gtatatattg aaaatacaag
     5521 atgaaggtaa aaatcatgct ataataaaat agtcaaaatt tttgccgata tttcagttta
     5581 agaggagaga ttgaattgaa attaagagca aacataattg aaaaaagagg aacattacaa
     5641 gtagttgtat cttataaaga ctctaacgga aaaattagac aaaagtggaa agatacaaaa
     5701 ttaaaaaaga aaggaaataa aaaacgagca gaaaaaatta gagatgaatt tttactagaa
     5761 ttggaaagtc aattagctat ggaatgtgaa tgtgctaatc caaatttatt gttttatgac
     5821 tatttgctta attatttgga agtatcaaaa aaacaagtag catttaacac atatgttggg
     5881 tataagcatt acgtccataa tcgaatttac aagttttttt atcctaaaaa aattaaacta
     5941 aataggttaa agccttatca tttacaagag ttttatcaat atatgttaaa tgataactgt
     6001 acagcaaata cggttattca ttatcacgca tttatacgca aagctcttca agaggctgtt
     6061 attacagaat tgataagtac aaatgtagca gataaagtaa gaaagccaaa aaagaaacaa
     6121 tttgtttctc aagtttataa tcaacaagag atcttgaaat tacttgacat tttgtcagaa
     6181 gaaaaactct atttggtagt tcttttaaca gcattttatg gattgcgaag aagtgaagtt
     6241 ttaggattaa aatggtctgc tattgatttt ttaaacaaaa aaatcatgat aaaccatgta
     6301 atcatagaaa atccagagaa tatgtcacag ttaataaaga aagatcaaac aaagaactct
     6361 tccagctatc gtacattacc tttggttgaa acgattgaac gagcattagt aaatcaagct
     6421 aaatggcaac aagataatcg tattctttta ggagatgatt atattttaga agataatgag
     6481 tacgtattta ctatggaaga tggacggttg atgaagcctc aatatgtaac gcatcgtttg
     6541 agtaagttga ttaagaaaaa tggattgaaa aaaattcgct ttcatgactt gcgtcattct
     6601 aatgcttcaa taatgttaga tagtggccag aatatgaaat ccattcaaga atggctggga
     6661 catgcatcgt attcgacaac tgctaattta tacacgcatt taaccgcagg agtaaaagat
     6721 cgtgcagcag aagcattaga aaacgcattt gggtttaaag aataaaaaaa caaaaaacaa
     6781 tatctttggg gtatcgcttc ttgtttttag aatttttttg ttggcaattt gttggcagta
     6841 tgttggcaat gaaaaaattc acaaactaaa aacctattta taaagcttta taaaactttt
     6901 ttcacaaata gagcatttgg ttaagagtca gctgctctac caactgagct aacggcccgt
     6961 acaaaaagta cttaataata gtagcactaa gaattttaaa atgcaagact acatttaaag
     7021 aaaatcagaa aaatctttac ttccttatag gaaatcattc atagattatt cacatttgct
     7081 cgctattata taagcatacc aacaaacaaa gttcttgcaa gagaacaaac cctaacactt
     7141 ttttcatttt ttactccttt cccgccgtct ccccggcggg tctcctgttc ttaacgataa
     7201 aagacaagaa ggcttagcct tcttgttttt tgttttcatg gaatcccgct aatcaaatca
     7261 cctagttaaa gcaactagat tgaatgttta tcataagtct gatatcatgt ttatgaaatg
     7321 aaaaaaggag cggttatgat ggaaaaaatg cgtcagcaat taatggagtg ggggaaggaa
     7381 ctagaaacat tccgtctgcc tcactgggaa gaactccctg acttagaact ttatatggac
     7441 caagtcagaa cgttagttga tcgttatctt tcacctgtga tacaaggaga aaagcatcca
     7501 ttgcttacct cttctatggt aaacaattac gttaaactag gtttgatacc agcaccagtt
     7561 aaaaaaagat ataacaaaga acacgtagca tttttacttg caatcactac gttgaaacaa
     7621 gtattgacta ttcctgaaat caaagaaggg atattgttcc aaggaaaaac agtaggaatt
     7681 cgtgaagcct ataatctatt ttgcgatgaa caagaagcag ctgtgtggat ggtcagccaa
     7741 ttagcacaag gtaaatctca tccgcaaaaa ttcgaccagg caacccctgt agaatatatt
     7801 gctgtaagag cggcaacttt atcgtttgca atgaaacttc ttgcggaaaa aacgattgtt
     7861 ctagaaaccg aatatttaaa agaggagaaa acaaatgaaa aacaataaaa tcgcaatttt
     7921 agtagattca ggtacggatg ttccagaaga attgataaaa aaatacgaga tgtatatgat
     7981 cccattgaaa attatttata aagatcaggt atatacagat aaaattgaca ttacccctga
     8041 agagatctat caacgtttgc cagaggaaat ccctggtaca tctttgccgg atggtgagtc
     8101 gatcacagaa atctttgagc aaatcaaaaa agacggttat gaaaaggtac ttgcggtaac
     8161 aatttccagt ggattgagcg ggacatataa tgtggttcgt ttaattgcac aacagcaaga
     8221 agaattggaa gcatatgtac tagatacgaa aaatattggt attggtgcag gtttttctgc
     8281 gatccaagca gcaaaatggc tggaagaagg agtagaatgg gatcaactga tcagtaatct
     8341 gaatgagtta gtcgaaagaa caaaagtttt ctttaatgtt gcgacattgg aatatcttca
     8401 aaaaggtgga cggatcggtt tagttgcttc tattgtagga accgctttaa agcttaaccc
     8461 aatcatctca tgtaatgaag aagggattta ttacacagta ggaaaagcta ggggacgtaa
     8521 aaaaagtttg gataaaacgg ttgcttacgt caaagaaaaa atcggtaatg caaaggtctt
     8581 caacttagct gttgcccatg gggatgcgaa agaagaagca attgaaatgg aagcgagact
     8641 taaaagagag ttcccacaag caaaccaaat ctactttggc cagatttctc cggcattagt
     8701 agtccacact ggtccgggat tattgggtgt cggtgtacaa gttttagaag gataaatcaa
     8761 aaaaaggaaa cactgaggtg aaaatcagtg ttttcctttt ttattgaagt tagaacaaaa
     8821 aataataaag tattttgcac tttcatttta attttctctt ttgttttata ggcaaagatg
     8881 aaacaaaagc agaaatacgg tataatgaga aggtatgaca ataatatata agaggtggct
     8941 ctttatgaaa aaagttttag ttgtcgatga tgagaagccg atttcggata tcgtcaaatt
     9001 caatttagcc aaagaaggct atgacgttta tactgcatat gatggggaag aagccttgga
     9061 aaaagttgct gaggtagaac ctgatttgat tttacttgat ttgatgctac ctaaaatgga
     9121 cggcttagaa gttgctcgtg aggtacgcaa aacttatgat atgccgatca ttatggtaac
     9181 agccaaagat tctgaaattg ataaagtcct aggcttagag ttaggcgccg atgattacgt
     9241 gaccaagcca ttttccaacc gagaattagt tgcacgtgtc aaagcaaatc ttcgtcgcgg
     9301 tgccacggct gcgaaggaac cagaagaggc agcacctgcg gaattgacga ttggtgattt
     9361 gacgatccat ccagaagctt acatggtgac gaaacgagga gaaacaatcg agttaacaca
     9421 tcgtgaattc gaactactct tttacttggc gaagcacctt ggacaagtga tgacaagaga
     9481 acatttatta caaacggttt ggggttatga ttattttgga gatgtacgaa cagtggatgt
     9541 aactgtacgt cgtttgcgtg aaaagatcga agataatcca agccatccaa attatttagt
     9601 gacacgtcgt ggtgtcggtt actatttgcg taacccagaa caggagtaat tggatgaaaa
     9661 gaaaagtccg cttttttcga tcagtcaatt ttaagatcgc tattaccttt attctgattt
     9721 tattgatctc gatcgaaatt attggtgcct actttatacg tggtttagaa cgttccacga
     9781 tcaatacatt tattaaagat atgaatcaaa cggtggaatc attagcaaca acaatcagcc
     9841 cggaattgaa tcgaaaagat aatgcagatg atgaagaggt aaatgcgaat atcaaaagat
     9901 ttattgaaaa cagtgcgact agcgatatca tcgaaatacg ggtcgttgac gaaaaaggga
     9961 tcatccgagg aacaaccgat gtcaatgaac aaagcgctgt gggaaagaaa aatgattatg
    10021 ttgatatcaa tgatttcacg actaaacgtt atgttgcttt agataacgac gataaacggg
    10081 tgaatatcaa tgttcaaccg atcctgtccc cgaccggcga tgccgttatc ggggctcttt
    10141 acgtcaaaag taatatcgaa caaaaatatt cggaaatcaa taacacagca gtcattttct
    10201 ttactgcctc actaattgcg gcatttattt caatgatcgt atctgtttta gttgctcgtt
    10261 cgattacaca gccgatcggt gaaatgagag aacaagccat tcgaatcgct agaggagact
    10321 acagtcggaa agtcaaagtg catggacaag atgaacttgg acagctggct gataccttca
    10381 atcagttagc tgaacgtatt gaagaagctc aagatacaat ggaagctgaa cgcaatcgtt
    10441 tggatagcgt gctatcccat atgacagacg gcgtcattgc gacagatcgc aggggtaaag
    10501 tcattacgat caatgatatg gctgtttcct tgctagatat caaaaacgag gaagcaatcg
    10561 gacaatcgat tttgacactt ttagatatcg aagaagaata tacgttgcgg aaattattag
    10621 aagatccaaa cgaaatggta cttgaccgtt caaccagtac attggaaagt gatcagatga
    10681 tcttgcgagt agacttttct atgatccgtc gcgaatcagg ttttatcagc ggattagtag
    10741 ctgtcttgca tgatgtgaca gaacaagaaa aaacagaacg ggaacgtcgt gaattcgtct
    10801 ctaatgtttc ccatgaactt cgtacaccgt tgacaagtat gcgtagttat atcgaagcat
    10861 tgagcgaagg tgcgtggaaa gacgaggaag tagcaccaaa ttttttgaag gtaacgctag
    10921 atgaaacaga ccgaatgatt cgaatgatca atgacctctt aaatctatct cgcatggata
    10981 caggtaatac ccagcttcaa ttagaatatg tcaatttcaa cgagttagtg aattttgttc
    11041 ttgaccggtt tgatatgatg gtgggaaatc aggaaaaaaa ttataagatc cgtcgtgaat
    11101 ttacgcaaag agatctttgg gtcgaagtag atacagataa aatcatccaa gtagtcgata
    11161 atatcatgaa taacgcgatc aagtattcac cagatggggg aacgattact tgtcgcttat
    11221 tagaaacgca taataacgtg attttaagta tcaccgatca aggattgggg attcctaaaa
    11281 aagatctgaa ccgagtgttc gagcggttct atcgagtaga taaagcacgt gcaagagcac
    11341 aaggtgggac aggacttgga ttagctattt ctagagaagt gattaaggcg catcgaggcg
    11401 ctatttgggc agagagcaag gaaggaaaag ggtcgacttt ttatatctcg ctgccatatg
    11461 aaccttatga ggaggaatgg tgggaatgag aatctctgaa aaagtcgttc gtataggact
    11521 cattttgatg gttgcgttaa gcatatactt ctcttatgca atctggctga gcccagcagg
    11581 aaaaagttca atcaacgtag atgagacaaa cactcaaatg atcgagtctc aaagcaatcg
    11641 aaaaacttcc gaagtctttc ttccgcttca tgttacatgg caccattcgg gaatgatcca
    11701 agaaaccaac agtgaaaatc tcgtgtaccg attgcaattg atcatcgata aagcaagatt
    11761 tggaaagctt tctgaagtag tttcaggaga caaatcacag tttgaacagg tgaaaaatct
    11821 gaactctggt tttgagttgg cctataatgc gccttttttg ctaagagaat acaaggaagc
    11881 atttgattta gatttagatg tgtcgaactt ggcaaaagaa agcgaaaata tgtattttac
    11941 tagaatacaa ttcgatcaag gaaataaaaa aatccgattt ttgaattatt cgaaccatct
    12001 cgtatatgaa gcaaatattt cgttgaattg gacagatatc aataaagaat taacagcaaa
    12061 agacacaaaa tggacagaaa tgacaacaga accatcgacg atcgacacgc aatatgctac
    12121 gaaacattcg atcaaactga agaagtatag ttatatcctg tcacagcaac cctatacact
    12181 ttttagaaat gcttttttcc agaaaccaga tagcgtgaaa aataatgatg aaagcaatga
    12241 attgattttt tacggtggga atgaaacaat gtctatccat tcggaaacgc aagtagttga
    12301 ttttcgtgga gagctgccag agaaaaaaga aaatgctagt gtattttctg aaagtttccc
    12361 ctatattagc aaattaggca gttctttagg gaacctcaga tattttgacc gcagtgacaa
    12421 tacgatcgat tatcggatat tcgtggaagg cttcccggtt tttgggacag acagcagagg
    12481 acagattggt gtggaagttg gcggtgaaac gaatggttct gtgcaagtga atatccaaac
    12541 cagtttgaat acgattcaag tgccgatacc ttcagacgaa gaagtagaac ttgcaagtac
    12601 gaatgaaatc atccagcagt taaaagctaa gggagcagat agcaacaaga ttggttcagt
    12661 gatcgttggc tatacatggc aaaatgtcaa agaaacgaat cgtgtcgttg atctcttacc
    12721 tgaatggtat gtgaaatata aagataactg gtattcagct aacgaccttc tggaaaaatt
    12781 acctggattg gaggcgaatt aattggattt caaaaaaatc gaatggatat ttttcgtcgc
    12841 ttttttaggg ttgaatgtgt ttttattcag cagttatcat gacgcggtat accaagaaaa
    12901 caatgtcatt cgctctaatc agactgaatc aatccaacaa cgtctggcta gcgacgatat
    12961 cacttacacg gggaagattt cgtctgagaa caagcaaggc tattatctta gcgcggagca
    13021 gaccaatctt agcacttcat tagcgaatta tcgcaaggct ggtaaaagcg gcttgctggc
    13081 aagtggtgtg tttttagacg gtgatgtttt gacgaaatct ttatccagtt cagatgctga
    13141 aaagaacgtg atcgatccag ataaaattag tagttccttg gaagattttt tatctaacaa
    13201 agacaatatt ttattcggta atgattatgt gtacacgaaa aacttttctc atttagaaga
    13261 agaaccacta gaaatccaag caagccaaga gtatgaaggc atcccattca atgatgatac
    13321 agcaaaaatc gttctttctt tagaaaaaaa cgataatgat tatagcatca cgaaatacat
    13381 gcagacgcat ttatctgata tcgagcagct gcgcgaaaag acggaactgt atacggaaga
    13441 ggaagcaatc aatacattgt acatcaataa caaaatacca agaggatcaa aaattttatg
    13501 gcgtcagcta gcttattcct gtattctgaa agtccgggaa aaaaatgtgt atgttcctgt
    13561 ttggcatgta gcgatcgaaa caagcgataa agtggtccaa attgagagcg tcaacgcttt
    13621 cagcaatacg atcgtgacaa acaatactgt accaaaagtg gaagatcaat aaaaaagcga
    13681 gtataatgaa aaaagtttac aaagtaagaa gggaagtagg agtcaaatgg ctgaagaaaa
    13741 accagctttt aaaatcagta tcctagcaag tggaagttcg ggtaattcac tttatatcga
    13801 aagtgaaaaa aaacggcttt tggtcgatgc aggacttagc ggtaaaaaaa tcacttcctt
    13861 gatggctcaa attgatcgga caccggacca attagatgga atacttgtga cgcatgaaca
    13921 tcgagatcat atacatggcg tgggtgtctt ggctcgtaag tatcatttgg atgtttttgc
    13981 caatcagcag acttgggaag cgatggaacc actgattgga aatgtaccag tggaacaaaa
    14041 acatctattt gagatgggga aagtcagaac attcggcgat atcgatattg agagtttcgg
    14101 cgtctcccat gacgcagcgg ctccgcaatt ttatcgtttc cataaagaaa atcggtcatt
    14161 cgtcatgttg acggacaccg gatattgcag tgattatatg cgaggtgtca ttgaaaatgc
    14221 cgatggttat ttgatggaaa gtaatcatga tcttgagatg ttacgcatgg gaccttatcc
    14281 gtggaattta aaacagcgta ttttaggaga tagaggacat ctttccaacg aagacggtgc
    14341 acttgtcatg actgatgtga tcggtaatcg gaccaaacga atctatcttg gccatcttag
    14401 caaggaaaac aatatgaaag aacttgctca tttaacaatg gaaaatgttt tgaaagaaaa
    14461 agattttggc gttggacatg attttgaaat ctatgataca gatccagatg aagcaactga
    14521 gctattttcg atttaaacag ttcaatcaat aaaaatataa aaagcagaag ataaaatcaa
    14581 tcagggttgg ttttatcttc tatttttatt tttttagcat attaaaaaaa taaccgtatt
    14641 atcgactgac tacactgatg tgctgctttt catgtttctc atccagtatc tcatctacta
    14701 atgcaatcgc gtaatctgcc atgctgatcg tactgtcgct agcagcgttt ctttgtaaat
    14761 aatcatcact taacaaataa gtacctgtac gtttgccatg tggatcaaat aaagaagctg
    14821 gactgatata agtccaatgt agatttttag attgtttcaa ttttaacaat ccttcgtaca
    14881 tattgaaagc tgttggatag aagggtgcat ttttaggtac ttggtcaatc atacgagtcg
    14941 tcttttctgt atctaagaat aacgaagagg cacccccaac gacaattaag cgtgtatcgg
    15001 ttccttcaag catctcaatc aaatgggaca aagaagtttg atgcatttct tcttttcctt
    15061 tgggagcgtt gaaagcatca acgacaacat caaaagatgc aagatcagta gttgtcaact
    15121 caaataaatc cttttgtata aacggaacat ctgatggcaa tttttctgga tgacgcacga
    15181 ttgccgtcac gtctaaatct ctggacaatg cttcgtctaa aataaatgaa cctgcatggc
    15241 cagtagctcc aatgagtgca attttcataa aaaacctcct tttttattcc tgtccattgt
    15301 aagcagttgc tgaatattcg tcaaagaatc cgatttttga aaagctcagt gcgcattcct
    15361 ttcatggttc gtgatcattg ggtatagtta aattaagaaa actgattgat aaaaatagaa
    15421 gttgaaggag ataagaaatg aaagagtggt tagatcgtgc ctttttacca atgataacat
    15481 ctgttgtgaa tcttagaccc ttaactgctt tgcgtaatgg aatgacttac acgatcccaa
    15541 taattattgt tggctcgctg tttttcttat ttgctcattt tcctgtggat tacatcgctc
    15601 agtggataga caaaacagga ttttctatgt attttacaca agtataccag gctacatttg
    15661 atgtgatagc cttttggatc gtttttgcca ttgcatacgc ttatgcaaaa gaagaagaca
    15721 tcgatcgctt accagtcggc ataacagcct tgtctgctta tctactgctt attcagccac
    15781 taaatactca gatagatact gtctggacag gtgctgaagg gatactagga gcagttacaa
    15841 ctgggctcat catcggatgg ggctatgcac gtttagtaag acttgcgaaa gggtggaagc
    15901 aaagagagaa gatacctgaa aatgtttggc agtcttttac ttcattgatt ccaatcattg
    15961 ttatagtcac ttttacgctt atcggttcag cggtttttca acatttgttc agaatgactg
    16021 ttgttcaagg agtatggaca tatgtccagc ttcccattca atcgttcata gacacgttgt
    16081 ttggtgtgct tttattaggt ttttttgttc cgtttttatg gttatttggt cttcatggat
    16141 ctgcgatggt caatgggttg gttagtcccg tcttgcaagc gaattcatta gcaaatgcag
    16201 aaatcttagc ctcaggacaa gagttgacgg tggcaaatgg cggacatatc gttactcagc
    16261 aatttctcga tcagttcatg acggttacgg gtgctggatt gacgttaggg gctgtatttt
    16321 ttatgacggt ttttgcgaaa tcgaagaaat acagagaatt aggaaatctt gctcttttac
    16381 cagccttttt caacatcaat gaatcaatta ttttttccac accgatcgtg atgaacccga
    16441 tgatggctgt tccttttatt ttcgcaccgg tcgtttcagg attgattacc tattccgctt
    16501 tatattttgg attcgttcct ttatttacag cagtccaagt cccttggaca acaccgccga
    16561 tcctctcggg tttcttagta ggcggagtgc ctgcagcgat tttacaaggt atcgtgttgt
    16621 caattagttt tttcatttat ttcccatttt tgaaaaaaat agattctgca aactgcaaaa
    16681 aggaaagaca gacaaaaaat gatggagcag cagaaaagat gaacgactaa aaaacaaaaa
    16741 tactcctgtc gctttagatt gggaaaatca ccaatctagg atgacaggag tatttatagt
    16801 catgtttata ctagcccttt ttggacagct aattgaacaa gaacaactaa tgtgttcatt
    16861 cctacgtgtg tgatcatcga tgtccagatc cgaccagttg ctttgtactc taggctgaag
    16921 aagaaaccta ggaaaaagta gattagcaga tgaccatcat tatgagcaaa cgcaaacagc
    16981 aaagagctga tcactgctcc gacccaaaaa tttgaatatc gactgatgat ccccagtata
    17041 ctgcggcgga atacgaactc ttccatgatg ggtccagcaa cagttgtcgc caaaataaaa
    17101 gcgggtgctt ccatgatcat ctggatgata ttttgtgtat tctcggaagg ggttgctttg
    17161 ccaaacaata cagattctaa catgacagcg atgctttgca gaatcaatgc gacaaagatt
    17221 ccaaccaatc cataaataac aatctttgga gcagaagctt tttttaccgc tgattcgatc
    17281 gctaagggtt cacgttgctt aaaataaaaa atagctagga caacggcgcc tagtatatac
    17341 atgactgcag ttgctgtgat caattcagac gtagatttta caagtcctat cgaagctagg
    17401 atcaagggtg agataaaaat tagaccatag caaaaaatgc tgaggaagct atattttttt
    17461 aaagacatgt aattcctcct tttaagggat aacaagcatg caaggcaagc ttatatcgat
    17521 tgatttcaat tatatcataa tttcaataag cagctgtcag aataaaattt cttttctttg
    17581 atttaaaagc ttgcaaaaaa gagggagaat gagtattatt aaaaatgtag gttagcactc
    17641 gaaggttaag agtgctaaca gattaaaaaa agagttatcg gagggattta tcgtgttaaa
    17701 accattaggt gatcgcgtca ttatcgaagt cgcaaaagaa gaagaaaaaa ctgtcggagg
    17761 aatcgtttta gcttctgctg ctaaagagaa accccaaact gggacagttg ttgcagtcgg
    17821 tgaaggccgt ttgttagaaa acggtgaaaa agttcctgcc gctgtaaaag caggcgacca
    17881 agttatgttc gaaaaatacg ccggtacaga agtaaaatac gaaggaaaag aatatctgat
    17941 cgtcgcagga aaagatatca tggcgattgt tgagtaaatt tggaagataa aaaaataatc
    18001 aaaaataatc aatgaggtga atcaatcatg gcaaaagaat tgaaatttgc agaagacgca
    18061 cgcgcagcta tgttacgcgg ggtagataaa ttagcagata cagtgaaagt aacattagga
    18121 cctaaaggcc gaaatgtcgt actagaaaaa tcttacggct ctccattgat cacaaatgat
    18181 ggtgtgacga tcgcaaaaga aatcgaatta gaagatcatt ttgaaaacat gggtgcaaaa
    18241 cttgtatcag aagtagcatc aaaaacaaat gatattgcag gagacggtac aacgacagca
    18301 actgttctga cacaagcaat cgttcgcgaa ggtttaaaaa acgtaactgc aggtgctaat
    18361 cctctaggca ttcgtcgcgg gatcgaattg gcaacaaaag cagcagtcga agaattgcat
    18421 aatatctcaa ctgttgttga ttcaaaagaa gccattgcac aagtagcagc tgtttcttcg
    18481 ggttctgata aagttggaca tttgattgct gatgcaatgg aaaaagttgg aaacgacggt
    18541 gtcatcacaa tcgaagaatc aaaagggatc gaaacagaac tagatgttgt agaaggtatg
    18601 cagtttgatc gtggttactt atcacaatat atggtaacag ataacgacaa aatggaagct
    18661 gttctagaaa acccatatat cttgatcaca gacaaaaaaa tctctaacat ccaagatatt
    18721 ctgccattgt tagaacaaat cttgcaacaa tcacgtccat tgttgattat tgctgacgat
    18781 gtagatggtg aagcattgcc aactcttgta ttgaacaaaa tccgtggaac attcaacgta
    18841 gtagctgtaa aagctcctgg tttcggcgat cgtcgtaaag caatgcttga agatatcgct
    18901 attttgacag gtggtacagt aattactgac gatctaggtt tagaattgaa agatgcaaca
    18961 atcgaaaact taggaaatgc ttctaaagtt gttgtagata aagacaatac aacgatcgta
    19021 gaaggttctg gagaaaaaga agcaatcgaa gctcgtgtgc aattgatcaa aaaccaaatt
    19081 gctgaaacaa cttctgattt tgaccgcgaa aaattacaag aacgcttagc taaattagca
    19141 ggtggtgtag ctgttgttaa agttggtgca gctactgaaa cagaattgaa agaattaaaa
    19201 ttacgcattg aagatgcatt gaatgctaca cgtgccgcag tagaagaagg aatggtctca
    19261 ggtggtggta cagctctagt gaacgttatc agcaaagttt ctgctgtaga agcagaagga
    19321 gacgtggcaa caggtatcaa gatcgttgtt cgtgctttag aagaaccaat tcgtcaaatc
    19381 gctgaaaacg ctggttatga aggatcagtg attgttgata aattgaagaa cgtggaacta
    19441 ggcactggtt tcaatgctgc tactggagaa tgggtaaaca tggttgaagc aggtatcgta
    19501 gacccaacta aagtaacacg ttctgccttg caaaatgccg cttccgtttc tgccttgtta
    19561 ttaacgacag aagcagtcgt tgcagataaa ccagaaccag cagcgccagc agctcctgca
    19621 atggatccat caatgatggg cggtatgatg tagtaattaa gaaagaaccc tattttaaca
    19681 gggttctttc ttcttttttt gttttatttt aagcaaaagg ggcaaagaag gggcaaaaat
    19741 atcaaaggct atttagttta tcaattactt gttgtttggc tctttttgtt acatggttat
    19801 aaatagacag agttgtgttt gcatcagaat gccctacacg ctccataatg gctttaagag
    19861 gtacgcctaa ttcagataat agtgaaacgt ggctatgtct gaatatatgt gaggatagac
    19921 ttttttctaa ttccaactct tcctctactt tatggagtat agcgttaaat gagtgcagcg
    19981 caagcggagt gccacttgta gatataaata tatattgatc gggatctgtg ggtctacctg
    20041 caagaatatt gtcagctatc acactttcaa tcaattcttt tgcacgattg ggtaattgca
    20101 cttcacgttg cgaataagta ttttttggag ttgtttttat agcattatcc attttcacag
    20161 atgtgtaatc taaggtccca ttaatggaaa ttttcccatc ctcatagtcc ttcatttgca
    20221 aagctagcaa ttccccatat ctcaaaccag ttaaatatag aaactcagct attatgccgt
    20281 gtagttttct gcgaggattg gagtatagct gtttcagtat ttgatcaatt tcttctttct
    20341 ccagatattt tttattcata gaaagccttc ttttttcttc ttccactttt ttaggatgga
    20401 ttttaactgc tagcgcaggg tttctttgaa tgtattttct atcgattgca tagtttagca
    20461 taacagatag agttgttttt gtttgttttg tgtagttcag tgagaggtca ccaaacgtat
    20521 acatatcttc aattatctta ttaatgagtg tctcatcaat gtttctaacg atcgtatcat
    20581 cgcttatgtg cttagaaaca tgtttcatca tcattggaac cttcaaatag ctagtacgtt
    20641 taacatgctg cttataatat tcataccatt ctttatacag ctcaccaaaa gtgatatctg
    20701 atttattgta atcttcgagt gcttctttaa tttttttatc tagaattttc tgagcttttt
    20761 tccacgcttg tggtgaatta cttgtaagtg ttgtagattt ttttcgtgtt ttttctgtat
    20821 aaggatctac atatctttca ataaacttga attggccatc tttggtctgt tcaacccaca
    20881 cttttaacat ctcctatcat ttgctataat aggcataaca aatagaccta tataggtttg
    20941 ttttctaaaa gcacgctctt actctggacg gtggggcgtg ttttttattt attatacagt
    21001 tgctttaaaa atagctgctt tttcaaatga tactaaagga gagaagtgga tttctatttc
    21061 tcccttttgg tttaacgtga aatgagcagt tacatccatt tgttttcctg gagcaacaga
    21121 tcccattgta ttttcgttgg catatgtctc tgattttttg tcatctggtc catatacttc
    21181 cacatctgta cctacaggga tatctgaatc gccatcattt tttacggtat aagtaatttt
    21241 tactacttgt gcaggttggt tttcttcaaa ttgatttctt tcatcagtta gttctacact
    21301 atttagtgta tattcagcat caccaacttt tacagtgtca ccaatcttat agaaggtatc
    21361 gcttttttct tctttagaag atgaagcaga ggttgaagat ttagttactt tttcgccacc
    21421 attatcactt gctttattac tatctgatcc accatttaaa gcagaaccaa taattataat
    21481 taaaattact gctaataccc aaaaccatac ttttttgtaa aaaggtttac taactttata
    21541 catttttccg tcttgaccca taactttttt tgccatttaa atattcctca cttcttgtta
    21601 taatatattt gcgatctcag aaatgaggta tgagtccgtg tgccagcacg ggctttttta
    21661 atgttttgct gaaatcggtt tttttaataa ttcttgatgt attcatacat attgccttgc
    21721 gtaagaatat tttttcttaa aatagcattg gcagacagca taacaagtgt atctgagctt
    21781 attctgaata gaataatact ccataaattt ttcgagatta aactgagatt catcagtcag
    21841 ttcgttctca atatagatat ttaataaaat tagaatagct attttatctg cttctgtttc
    21901 aaattttgag tgaaaagttg tagaagtatc gtacaaaact gaatattcaa aatgagaagc
    21961 ggtgaaatgc gcaagttcat gagataaatg aaaggcttct gcagtttcgc catatagatt
    22021 ttcattcaaa aaaatgattc taggcttcgg ataatagaag ccaggctctt tcatttccat
    22081 gtaaactact ttcaaattgt attcgctcaa catttctttc aatttcaaat acatacaacc
    22141 catcactcca actattcatt ttcctctaaa gctttagcaa ttgcaatcgc tttacgcatt
    22201 gtctccttag atatttcttt tccatcaaaa gaaaaaacag tatcgttttc tgataaatcc
    22261 acatgtttag gggtttctct ttcttctcta cctagaaggt agtctacaga gactccaaag
    22321 taatcagcta accgagctaa ctcaatagaa ttaggagttt gtcttttcca acttcctaaa
    22381 tatccattcg aatacccgaa attcatttcg agctgtctaa tggataatcc tttttctttt
    22441 gctaactcct ttattatttc gtaagtattc attgataaat caacctttct gaatgcttac
    22501 aaaaaaagtt tagataaata atgtaaaaat agttgactta tatacattat taatctatac
    22561 tatgttttgt aaacaagttt aacaactaaa aagacaacaa aaaatactat tgattaataa
    22621 atgccaaccg ccaagaaagc tttttaaatc aaagtttata tgtcttattt aactatgcat
    22681 ttagtataga ttattaatct aataaagtca acgcagtttt ttaaaaagtt gttaaatttg
    22741 tttacgaata taaaagaaag gagagaagaa tatggaaaaa acagtctcgg caaaaatcga
    22801 agatttgaaa ttagacatac tgaaacaagc aaaagtggcg atggaacacg cggtagataa
    22861 agaagactct gccatggttg cagccatagc agagatttta gctcacgttt agtcatcgtt
    22921 tttacggtct tctggaagct gaccatagat aacagcataa tgaatatcta aataagcttg
    22981 aacaatttct tttggcgaaa ttgattcgcc tttagtagat acttgtgatt cgtgatttag
    23041 ccatgcaaca actaaatccg cagcgatttc aaacggcgtt acactttgtg tcagttgcca
    23101 taaagaattt cacaatatgt atggtcgtgg taataatacc aaagagcaat ttgaggaatt
    23161 tatggaaatt aaaaaataca aaaaatcatt tattaaagga ggctaaacaa tgaacaagga
    23221 aatgagccaa acaatcaagg tacaaaaaat gatagatgat ctaacacatg gaattgatag
    23281 ccaagccgat aaaatcatca aggaattgca aggacaaaaa gttaaggatg caaaaatgct
    23341 tctcaagact atcaactttg aaatgaatcc aacaaaaaga aagctcgccg atgtgttgga
    23401 agaaaagttg gcttccgcta tcaacgagca agaattacta tttgaaaccg atacttttaa
    23461 cagctgatgt tttatagatg acagtttcat ccgaatctat atcatcgccg agagaaaaga
    23521 atgttgcttt tgacaaaaag ccgataagac ctacaagcgg tcttgatgtt gcaagagtag
    23581 ataccccaat atcaccttgc aaggtattca cgtaaatttg agaagaatag aacattccat
    23641 ctttttcttc gatatttgac gcatcatcgt atccaacgac caaagtatcg gcaaatattt
    23701 tgattttttc tccatcaagc atagtaatta caaattctgg tttcataaat tttcacctcc
    23761 ttaacaatta tttcagcctg tcacactgat aaggaaatta taccaaagaa aggaatgaga
    23821 aatatgaata caccacaaat ttttaatttc gagcaaaacg aagttcgaac tattttagta
    23881 aatgatgaac catattttgt aggaaaagat gtagcagacg ttctaggata ttcgaatccg
    23941 caaaaagcta ttcgagatca tgtggacttg gaggataaga cgcagaacga ttcgttcacc
    24001 gtcaatggaa cagcagttgt tttgattaac gagtcaggcc tttacagttt aatcttaaaa
    24061 tcaaaacttc cctctgccaa aaaatttaaa cgttgggtaa cgagtgaagt ccttccaaca
    24121 attagaaaaa caggtagcta ttcaaacgta cctcaaagtt ttgcacaagc attgcgttta
    24181 gcagcggatt tagaagaaaa gaaccaatta ctcgaacaac aaattgccga gtacgaacca
    24241 aagattagct acttagatac gattctttca tcgacagata cggtagctac ttctcaaatt
    24301 gcagctgatt acggaatgtc ggcaattgct ctaaacaaat tgctaaacga gttaggtgtt
    24361 cagcataaag ttagcggaca atggatactt taccgaaaac atatgaacca aggatacaca
    24421 aaatcgcaca caagtgagat accgaaagcc gatggcggca ctaaagttgt aatgaatacc
    24481 aaatggacac agaaagggcg agtgtttatt tacaacttat taatcgcaga gggctattac
    24541 cctcaaatgg atttagagga aattggttag aaaggagttt tagtatgact gacattgcag
    24601 aaatcactca acgagataga gaaaaaatca aagaatatgt cgaaagttca aagttcttaa
    24661 cttacaccat gcttgctgaa agatttggaa ttagcaaaag ctacttatct ttaattttaa
    24721 acggtaaaaa gacttctgca gaagcaaaca gaattataga ttcgattatc actatgtacg
    24781 aattgtagag ggaggtaaag tcgttggaag ataaaatcat tcttacaaaa tcagagcttc
    24841 aagaaatgtt aaacatcgag tatggaacga taatttttag gagggataat aagtgaaaaa
    24901 accaacactt tcagagctga tagaagctgc tgagaaggca gtaaaaccag acgactggta
    24961 ccgacaaagt ttaatcttgg agaagttcca cggcatgtca aaaactactt tagttgaata
    25021 ctgcaaggaa atggaaacaa ttcctgaatt tgcagaagga atacttcgtc caggtcactc
    25081 aaccacattt attcattacc atacttttat ttggttttta aagtggaaag acgcaaacaa
    25141 atatcgtgta aaaagtttat cgccttcaga tgttttgaag gaggcaagtt aatgggcaaa
    25201 ttcaacagag cactagtatt cagcgcaccg ctaatcatct acgctttagg cctctgggga
    25261 agcagacaag cgttgatagg gacaatcgtt tacatggttt ggatttttat ggggcttgat
    25321 gaagctgagc acagagcgaa aaaagccagt cgggagggac tgactaaaat atgatttttt
    25381 tatttttgtt cctttgggct gctattgcgt acttcatcca gctccttgtt gtcaaagttg
    25441 ctcttatttc ttttaggaag aacaaaagaa aatagcgcta taaatgaaag ataagtagcc
    25501 gctacattaa actgttcttt tgaaaaagtt acagtcatcg gaatgataat tgatattgag
    25561 gaagtaatct taattaaaaa tatagcaata ttttcaacat cgcgttggtt tttatttaac
    25621 attgtccaac atgttttagc aacaataaaa ataattacac atgatattag cacacagact
    25681 gaaaaaagtt ttagaaatgc gatgtagtca gtgttattga actccttcaa aatactattt
    25741 ttattcaatg taataaaagg aattttttta attgaagaca ggaaagataa ggtaataaac
    25801 agaattatat atatggttaa agtcgcagta agaatgtaat tgagcttttt cctcatagaa
    25861 tcacctcact ttctaacgaa aattatacca aaaaggagag aagaaataat gcaagaatta
    25921 gtaattttga aaaataaaga agctgtaact acgagcttac aagtggcaga aagtttcaaa
    25981 aagaaacata agcatgtact agaagcaatt gaatcaataa aaagatcggt cgaaaattcg
    26041 gccaatgttg aagatgggtc caattttgga cagatgtttg tggaagggaa cgagccagac
    26101 tcatacggaa gaagtcggag agtttatttc atgaatagag acggattttc cttgctagct
    26161 atgggattca ctggaagtaa agcaataaat ttcaaactaa aatttattga agctttcaac
    26221 gaaatggaag atgttattcg gaagaatact gttcctcaaa caattgaaga catgatgatc
    26281 tatcaattag aagaaatgaa agatgttaaa aaagatgttt ccatgcttaa agatactatg
    26341 cgaattagcg gacaacaaga gtttgaaatt aagcaaaaag gaaatatgaa agttatgg
//
LOCUS       DARBEC010000032        25717 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.032, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000032 DARBEC010000000
VERSION     DARBEC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.032"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..560
                     /locus_tag="SCL80_001269"
     CDS             138..560
                     /locus_tag="SCL80_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107150.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQQYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            complement(726..1919)
                     /locus_tag="SCL80_001270"
     CDS             complement(726..1919)
                     /locus_tag="SCL80_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107151.1"
                     /translation="MTTYQRKVLVSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFVMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            2143..2520
                     /locus_tag="SCL80_001271"
     CDS             2143..2520
                     /locus_tag="SCL80_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1107152.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKKIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2508..2846
                     /locus_tag="SCL80_001272"
                     /pseudo
     CDS             2508..2846
                     /locus_tag="SCL80_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2980..3423
                     /locus_tag="SCL80_001273"
     CDS             2980..3423
                     /locus_tag="SCL80_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEG1107153.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     HSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            3684..4499
                     /locus_tag="SCL80_001274"
     CDS             3684..4499
                     /locus_tag="SCL80_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107154.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTDTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            4509..5171
                     /locus_tag="SCL80_001275"
     CDS             4509..5171
                     /locus_tag="SCL80_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1107155.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGCIVMIGMTEELAKENSFSPVRLFIKMFRQFWLKGFFYWLF
                     AWIVSVIMIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSTNCWYFQVRNPASKPNQVL
                     RIAFYYTLKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTM
                     DL"
     gene            5379..7043
                     /gene="alsS"
                     /locus_tag="SCL80_001276"
     CDS             5379..7043
                     /gene="alsS"
                     /locus_tag="SCL80_001276"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEG1107156.1"
                     /translation="MAEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVEKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAKLTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            7056..7766
                     /gene="budA"
                     /locus_tag="SCL80_001277"
     CDS             7056..7766
                     /gene="budA"
                     /locus_tag="SCL80_001277"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEG1107157.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNIQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            7896..8393
                     /locus_tag="SCL80_001278"
     CDS             7896..8393
                     /locus_tag="SCL80_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEG1107158.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            8578..8838
                     /locus_tag="SCL80_001279"
     CDS             8578..8838
                     /locus_tag="SCL80_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107159.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(9200..9439)
                     /locus_tag="SCL80_001280"
     CDS             complement(9200..9439)
                     /locus_tag="SCL80_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107160.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            9725..10039
                     /locus_tag="SCL80_001281"
     CDS             9725..10039
                     /locus_tag="SCL80_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107161.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
     gene            complement(10473..10839)
                     /gene="ssrA"
                     /locus_tag="SCL80_001282"
     tmRNA           complement(10473..10839)
                     /gene="ssrA"
                     /locus_tag="SCL80_001282"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(11010..11783)
                     /locus_tag="SCL80_001283"
     CDS             complement(11010..11783)
                     /locus_tag="SCL80_001283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1107162.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            11927..12277
                     /locus_tag="SCL80_001284"
     CDS             11927..12277
                     /locus_tag="SCL80_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEG1107163.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            12258..12974
                     /locus_tag="SCL80_001285"
     CDS             12258..12974
                     /locus_tag="SCL80_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107164.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAAVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            12974..14422
                     /locus_tag="SCL80_001286"
     CDS             12974..14422
                     /locus_tag="SCL80_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1107165.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(14492..15001)
                     /locus_tag="SCL80_001287"
     CDS             complement(14492..15001)
                     /locus_tag="SCL80_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107166.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(14991..15716)
                     /locus_tag="SCL80_001288"
     CDS             complement(14991..15716)
                     /locus_tag="SCL80_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107167.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWRLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
     gene            complement(15939..18059)
                     /locus_tag="SCL80_001289"
     CDS             complement(15939..18059)
                     /locus_tag="SCL80_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEG1107168.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLAKGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            18289..19467
                     /locus_tag="SCL80_001290"
     CDS             18289..19467
                     /locus_tag="SCL80_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEG1107169.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIVGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            19483..20241
                     /gene="aroD"
                     /locus_tag="SCL80_001291"
     CDS             19483..20241
                     /gene="aroD"
                     /locus_tag="SCL80_001291"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEG1107170.1"
                     /translation="MVWVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLAGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            20264..20749
                     /gene="ybaK"
                     /locus_tag="SCL80_001292"
     CDS             20264..20749
                     /gene="ybaK"
                     /locus_tag="SCL80_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEG1107171.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(20820..22073)
                     /locus_tag="SCL80_001293"
     CDS             complement(20820..22073)
                     /locus_tag="SCL80_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1107172.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            22191..23540
                     /gene="gdhA"
                     /locus_tag="SCL80_001294"
     CDS             22191..23540
                     /gene="gdhA"
                     /locus_tag="SCL80_001294"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEG1107173.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            23652..24998
                     /locus_tag="SCL80_001295"
     CDS             23652..24998
                     /locus_tag="SCL80_001295"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEG1107174.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(25306..25692)
                     /locus_tag="SCL80_001296"
     CDS             complement(25306..25692)
                     /locus_tag="SCL80_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1107175.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         8513 a         4428 c         5135 g         7641 t
ORIGIN      
        1 ttcatatcaa tagaatactt aggaaaaaaa gagaaataat aacaggttta tgcactattg
       61 acggtgtgac aatatgatat ttgtatccgt ttttttcttg tatgtagtat agtaaagtag
      121 atacaggagg agaaaatatg cagacttttt taacaaaagt ttctcaaaag aaaattaaaa
      181 tgattcattt actgcttgaa acagaacgtt ggtgttcaat agaagaacta caaaacgaat
      241 tgaaggtatc aagtaagtct atcttgaatt atctaacaga tttagaagaa ttgttccaac
      301 aatatcctga taaggtcatt ttgaagaatg agaataatcg gcgcttttct atggaaaagc
      361 aggagaactt cccaatctac attatttatc ttcatttcta cagaaaatca tataattatc
      421 atttgattga attcatgtat caacatcctg agaaaaattt ggaagattat gcagacgctc
      481 aatatacgag tgtatccaca gtttttcgtt atgctaagtt attagtaaag tactttgaaa
      541 tgacggacgt caaagaatag tcgccgccag atagaaggaa attagcagtt gaaaaagctg
      601 ccctgcatca agtaatacga ccagccaaac ggaaacagtg tctcatgttt ttgatttggc
      661 tggtcttatt accgcagatg caagcttttt ccacacctct ttcttgtcat ttacagactc
      721 aactatcaat gctgcaagct ttcccttaat gctgtaacac cagtaagagt tgtcaagacc
      781 agcagactga ctaggtaaat accgctcaaa aacaagatta cggcagttaa actataatga
      841 tccatcaaga aaccgatgac gatagaagaa aaaccaccaa cagctcgtcc aaggttcata
      901 acgaaattat tcgccgtcgc gcggatttct gttggataca aactactaat cactgcgcca
      961 tatccaccat acatgccatt gataaagtag ccgactatca cagcggctgc taccaaactc
     1021 cactggctat gggctaacac aagtagatag acagataagg atgaacaaat taaaaacaaa
     1081 gaaaaggcta gtttaggccc caatttatcc atgattatgc caaaagtcat catccccaaa
     1141 gacatgccta aaatagtact gaccatccac aatgaagacc cagaaaccga aagccctaat
     1201 cgattttgca taattgaagg tagccaattc attaacccaa aatagccggc aatttggaca
     1261 gttaccatga tactaaggcc acatgtttgc caagcaagat gttttgattc aaacagtctg
     1321 ctaagtttgg ttttcttttc ggcagattgt acagatttga attcctttgg ttcagtcaag
     1381 ccgcgacgaa tgaacaagac catcaacacc ggcagtacac ctacgacata caacatcttc
     1441 caaccaaacg ttggaatgat cacagcagct aaaatcgcag ccaaagccgc accgacctgc
     1501 gcaccaatac cagcaaccga agaagctcgt ccaatttgtt tttttgaaaa actttcggaa
     1561 atcaaggaca tacacgcccc atattctcct cccgctccta cacctgcgat aaagcgaaag
     1621 agataaacca agtagatgtt tgaagcaaaa tacatcaata aagaagcaat cgaaaacaga
     1681 aggaccgttt gagaaaatac tttcacacgt ccatatttat cagctatgat gccaaaaaaa
     1741 atacctccaa tgagcatccc caaattagtg atcgttgaaa tgaaacccgc ttgcgtacca
     1801 ctaatatgaa aagaagtaat cattgaagat aatgaaaaag ctaaaaacat aatatccata
     1861 ttctctaaag caattcccgc cgatgtcgat actaatacct ttcgctgata cgttgtcatt
     1921 tttatacccc ttttcctaaa atttccctaa attgttaaaa gtatatccaa aaaagaataa
     1981 aaaaacaaga cttttttatt tgaaataata cacaaacaac cgatatagat aatccagctt
     2041 agttttaata gaaatgagta ttgacttgga gtacacttaa agttgtagaa taaataaaat
     2101 acataagtaa agtggcaaga aattaagaga aagagtgatc agatgaatat caaacaagtc
     2161 agtgaagaaa agggcatttc ggccgataca ttgcgctatt atgaacgaat cgggttgatt
     2221 ccaccagtta atcggacaaa aggcggaatc cgtgattata cagaagaaga cctaaaatgg
     2281 gtcgacttta cgatctgtat gcgaaatgcc ggattatcga tcgagtcttt gattgaatat
     2341 attcgcctat ctagcgaagg agaagcaact gtatttgagc ggcgtaagct tttgattgaa
     2401 gaaagtgaac ttttgacgaa aaaaattgct gaaatgcaag aatgtttgga acgtttacaa
     2461 ggaaaaatca agaaatatga tcgtgtgtta tctagaaata aaatggaatg tatcaagtaa
     2521 aaaaatcaag gagcatcgct atggagttac gcttactaaa atatttttgg acaattgcag
     2581 aagaaggaac aatctctcaa gccgcagaag ttctacatct tacacaacca accttaagcc
     2641 gacaactgaa ggagttagaa gagtaactag gaacagaact gtttgtacgc gaaaaccgca
     2701 aaatgatctt gacagaagca ggttattttc taaaaaagcc gagcagaaga aattttagat
     2761 ttaacgaatc aaaccactca agaattcgag aacagaaaaa aacaattatt cagcggccga
     2821 tttacgatcg gctgtgtgga agctgatagt tcgaatacat tggcactatt actagaaaag
     2881 ttgatccgtg attatcccag cgttcaattt catatccaca gcggaacaag tgatgatatc
     2941 acggagtgac tagaaaaaag cctgttagat ttagcgattt tgctagatcc agtagataca
     3001 gagaaatacg aaacgatttt gttaccaaga aaggaaaaat ggggattgct cattttagaa
     3061 aattctgttt tagcaaaaaa agacgtgata acaaaagaag actttgcgaa tgtacctctt
     3121 ttgatctcaa agcgtccaga aatacagcga atgtttgctg actggttagg cataaaaaag
     3181 gagcagttgc tgatcacagg aacgtacaat ttgattttca atgtatttgc gctaattgag
     3241 aatcaggtag gtgctgcttt agtcatagaa ggtgctgtaa gtcatcattc aaagcgagtg
     3301 acattcgttc cgcttgaccc aatgattgaa acgaactgca tgctagtctg gaaaaagaat
     3361 cggattttat cacctgtagt caatgaattg attcaccgtt tcaaagaagc ttttgaacag
     3421 taataagtat ctctaatcga agaaatttca atacaaaagc gagacgtatg acagtcttgc
     3481 ttttgtattt tttcattttt aaatcaaaaa aataaagaga tttacataac ttgttaaata
     3541 agcgcttaca aaaataagaa gaacggaaat gaatcagcta taataaccgt tgttctggat
     3601 tgtaatcatt acaaagaaat gaagattata actttggagg tgttcgaaat gaaagaaaaa
     3661 aagcaaaaat tcgttcaaaa aacatgccct ttattcaatg gttgggtatg attctgctga
     3721 ttgtattagc tttattgacg atcattcctt ttttatggat ggtttctgct tcattcaaag
     3781 caaataatga agtgttcacg attccgattc aatggatccc taaaacttgg catttagaga
     3841 attatctggt tatttggtct cgtattcctt tagtgacatt ttttaagaat acagcttttt
     3901 taagcgttgt gatcacgttg attcagttgt ttacttctag ttttgctgct tacgggtttt
     3961 ctaaactaaa ttttaaaggg cgcgatattt tatttttagc atatatcggc acgattgctg
     4021 tcccttggca gtcttatatg atcccgcagt tcattatgat gagaaaagtg ggtttaacag
     4081 atacgttgtg gtcgttggtg ctattacaag cgttcaatgc ttttggtgtg ttcttgttga
     4141 agcaatatta cagtagtatc ccagattctt tatgtgagag tgcgcggatc gatggcttat
     4201 cagagtgggg aatctatcgc aagatcattt tacctcttac aaaaccggcg attgccagtt
     4261 tgacgatcat cacattcgtt aatacatgga acgattatat ggggccattc atttatttgt
     4321 cttctacgga aaataaaaca atccagttag ggttgaaaat gtttattggg ctatttgatg
     4381 cagagtacgc attaatcatg gctgcctcag ttgtttctgt tcttcctgtt gcttttatat
     4441 ttttagcgat gcaaaaatat tttgtagaag ggatcgcttc atcaggaatg aaggaatagg
     4501 aggtgattat gaaagagcag cgttttgaca aaaatgttta tatggaattc ttttattggt
     4561 tgtatcaatc attggcaagt agcctttgtt tttggtgtgt caatatcttg tatatagcag
     4621 ctgcgttcct tttgtcgata gatggaagga atgtcttttc ctttattttt tctttacttt
     4681 ttattggtcc aggttgcatc gtgatgatag gtatgacaga agaattagca aaagaaaata
     4741 gtttttcacc agtacgtttg ttcataaaga tgtttcgcca attttggcta aagggatttt
     4801 tttattggct atttgcttgg atagttagtg tgataatgat ttttgattgt ttctttttta
     4861 tacgtttttc ttatggaaaa tggttgattc cgttgtttgt tttacttgct tgtttatcag
     4921 tttcctttag caccaactgc tggtattttc aggtgagaaa tccagcgtcg aaaccaaatc
     4981 aagttctacg gatagcgttc tactatacgt tgaaaaaatg gtatgtgagt ttactagatt
     5041 ttttactatt gacaagtttg tttctcttct ttttcgtaaa gccccaatgg tgtattctac
     5101 taggtccttc tatcgtattt ggtttgattt acttcaacaa tcgtaaattg atgagaacta
     5161 tggatctgta gactttataa attgaaactt tcaattaaga atgacagttt tttttatgta
     5221 aaaactgtca ttctttttca ttttttgaaa catatggcta aacaaatgaa ataaaacgtg
     5281 ctatactttt gtatgaaaca ggtttagaaa ctgtatcact tatttgtgat tatttctaac
     5341 taataattaa aaataaagca taaaaaggac gatgttcaat ggctgaaaaa atgaaacaag
     5401 gttcgaaatc tgtagtagaa agtttgatta atcatcatgt tgattatgta ttcggtatac
     5461 caggtgcaaa aatcgatggt gtatttaatg aattagagga tcaaggtcct gaattaatcg
     5521 tgactcgtca cgaacaaaat gctgcattta tggcacaagc aatcggacgt attacaggag
     5581 agccaggagt agttatcgca acaagtggtc ctggtgctag taacttagct acaggtttgg
     5641 taacagcgac agcagaaggt gacccagttt tagcaattgc cggacaagta aaacgaagcg
     5701 atctattgaa gttgacccat caaagcatgg ataatgcagc actgttccag ccaatcacaa
     5761 aatacagtgc agaaatccaa gatccagaaa caatttcaga agtgattgcc aatgcttatc
     5821 gtatggctaa gagttcaaaa aaaggcgcga gctttatcag tatcccgcaa gatgtagtgg
     5881 atgcacctgt ccaaggtaat gtcatcaagc cattgagcga tcctaaatta ggttctgctt
     5941 ctgcagatga tatccgttac ctagctgaac gcattagaga agcaaaacta ccggtactat
     6001 tagtaggtat gcgaggatca agtgaaaaag aaacgttagc catcaggcaa ttagttgaaa
     6061 agacagcatt gcctgttgta gagacattcc aagcagctgg agtgatctca cgtgaattag
     6121 aagcgcattt ctttggtcgt gtgggtttat ttagaaacca accaggagat atgcttttga
     6181 aacgaagtga tctagttatt gcaattggct atgatccaat cgaatatgaa gcaagaaact
     6241 ggaatgcgga aaaagatgcg cggatcattg tgatcgatga agcgccagct gaaatcgatc
     6301 cttttatgca gcctgaaaga gaattgatcg gtgatatctc agccactctt gatttattga
     6361 caggttcatt agagcctcaa caagtgtcag aagatgcaaa agaatattta gcatctcttc
     6421 aagcaaaatt aacggagcgg gacattgttc aatcaaaagg agaagcaggt atcttgcatc
     6481 ctttagaagt tattaacacg cttcagtcaa aagtaacaga tgatatgact gttaccgtag
     6541 atgtaggtag tcattatatt tggatggcac gccactttag aagttatgaa cctcgtcatt
     6601 tgttattcag taatggtatg caaacgcttg gtgttgcatt gccatgggcg atttctgctg
     6661 cattggtgcg tccaaataca caaatcgtat cagtttccgg cgatggggga tttttattct
     6721 ctgcccagga tttagaaacg gcggtacgta aaaaattgaa tatcgttcat ttgatttgga
     6781 atgatggaca ctacaatatg gtagagttcc aagaaaaaat gaaatatcag cgggcgtcag
     6841 gtgtagactt tggtccagtt gattttgtga aatatgcaga agcttttggc gctaaaggaa
     6901 tccgagctac aagtgtagag gaattagaaa aagctttaga agaaggattt gcgacagaag
     6961 gacccgtaat tattgatatt ccgattgatt accgtgataa tgagaaattg ggagaaacaa
     7021 tcttaccaga tcaattctat taaggaagtg tcgacatgac tgaaaaaatc ttataccagc
     7081 acgggacatt aggagctttg atggccggct tgatggatgg aacagaaacg attgctcata
     7141 tattagaaaa aggaactctg ggattgggaa cattgcatgg gcttgatggt gaagtcatct
     7201 ttttagatgg cgtagcttat caaggaagat cagatggctc agttgcccaa ttagatggaa
     7261 gtgaacttac cccctatgca gcaattacag attttacgcc agatactagt ttttctgtct
     7321 caaaaactgc agatggagaa caattgaaaa aagacatatt agtaagagaa gccggggaaa
     7381 acctattttt ggcagtgaag ataactggat tattcaaaaa tatgcacatc cgtatcatgc
     7441 cgaagcaaga aaaaccttac agaagactag caaaaatttc agagtctcaa ccagaattcc
     7501 aacaaagcaa tatccaagga acattagtcg ggttctttac gcctgaatta ttccaaggag
     7561 ttgctgcagc gggatttcac ctgcatttta tcgatgatac acatacattt ggcggacatg
     7621 taatggattt tgaagtggct gaaggaaaag ttgagattag ccgcatctcg tcccttgtac
     7681 agcattttcc agttgatgat ccgacttttg ttcacgaaac gattgattat gctgatttag
     7741 cagcagaaat ccaacaagca gaatagaaaa agacagcgag atgcgttcaa ggtgattaac
     7801 ctgacgtatc tcgttttttt gaggaggaaa cctagcgttt acaaaaaaac tgagctataa
     7861 tcttctccat agatcaaaaa ggagcggttt ttatcatgtg gacagaaaaa cagaaaaaag
     7921 atgcatacga aattttatta ttacagcaac aaggattatt agaagcagaa gataactggt
     7981 tagcaaattt agcaaattca tctgcattgt tgaatgagac tttgcctgag actgtctttg
     8041 caggatatta tttattcgat ggtaatgaat tgattttagg accgttccaa gggcgagttt
     8101 cttgcacacg tatcaagatg ggaaaaggag tttgtggaga atcagctgaa aaacaagaaa
     8161 cattgatcgt tgatgatgtc aaaacccatg caaattacat ttcctgcgat tcagccgcac
     8221 aatcagaaat cgtagtgcca atggtaaaag aaggtaattt aatcggtgtt ttagatatcg
     8281 attgcggagt aacgaaaggg tatgatgaaa tcgatcaagc atttctagaa aaatatgtag
     8341 atcttttatt agcaaatttt aatataaatg ctttttcatt tattaataac taaaatcgtc
     8401 tattgaaaca aaaaagaaga gaaatcaaga ataaaaagag ccggaaagac agtggtcatt
     8461 tctgtttttc ggttttttta tttttttata aaatcacaag tgttattgat tttcacaatc
     8521 atattttaat ggtattatca ttttgaataa tgaaaaaaca ttaaggatgg atgaaatatg
     8581 aataacgaaa ctatcatttt aggaaaaaaa tatatttgcc aacctattgg cttgaaacaa
     8641 aaagtggttg gagaagtaat cagtaaatta gaaaattgtg ttgtgatctg cgtagacgaa
     8701 tatcatgacg tcgatcgcaa tgaaatatta gaaaaatgcg gaaaagtagt ggcaaaatat
     8761 actcacgttt atgagttgga aacaacagaa tatctatata actatagcaa caaatatgaa
     8821 catactttag tttcataaag atttaaataa agtgccttca aaagcgagaa gaccaagctt
     8881 ttgaaggcac tttatttagt tggcagtgat tgttcatgat ttttgattta ttcaaaaggt
     8941 tcgcctagag aatctgtctc tgtaacgagg cctggtggag aaactcgata aaaacctata
     9001 ctgccagaac tgtcctattt tcggatagac aaactagtag ttttaaaaag atcatccgat
     9061 ccaatcattt caaaaaatgc aaaactgata tcaggatcat ttagctcagg cttgttatcg
     9121 taaagcattt gtaaaggttg catttagtta ggagtaaacg cgaaactatc atcattattt
     9181 gctgaagaag atgaagaagc taattcagag tttgtttgaa ataaaggaga cgtgctgaca
     9241 gtttctttac cctgtttttt gcagaggtag aaacttttat atcggaagtt tctaccggag
     9301 aatttttctc atttgtagaa attgttgaat cgatactatt cgaaatttgc gtatctgaag
     9361 gtatgttctt tgatgcagaa gtcccacaag cagtaagtaa caataaattt gataaactga
     9421 taaacagaat tcttttcatt atgtacacac tccttatagt atcgtttagc atacacctat
     9481 gctcagtatt aggcaaaatc aatgcaaaac tatcaagtct tttatagtat ctattagtct
     9541 tagtaataca tatatggaga gggtgatatc ccagttcaaa ccagatacat tggttaccat
     9601 ctgtattaac gattatgcct ctaaacgctt tttttatgaa aaaatttata agtgaaaacg
     9661 taggtatgat attatctaga ggtaattttt ttgggtattt aaatagttgc taggaggata
     9721 gcttttggat ttttacagta attttatctt aataatagca atattattac ttctaaatat
     9781 ttggtttttt gataagagta gaaatgcagg gataggtttt agaaccaaac gaagtacgtc
     9841 ttctgaaaaa aagtgggtat attcacagac tattttttac ggaggggtta tttcaatcag
     9901 tttactttct tcaacacttt attcttttaa tgttatagac gtttccatgt caaattttat
     9961 atctattatt ggaatcctta tttctgcaat tattacacaa ctacttcttg tgtttgaaga
    10021 gaagtcaaaa aataattaaa gcaattatgc cttttagtac aggaattatt acttcttaca
    10081 aatgtatagc gaatgtgaag cctgctgttt taattgttct agaaaaactt ttgagtgaac
    10141 aattatttta tgttaggatg aattaatttc tctagagttt ataattaaaa aacttatttg
    10201 aaggaacatg cttaaaccat aattgtgttt ttccttccat cagcataaaa gttcacctca
    10261 aaaagagtgt acgaacaaaa gttctttttg taaaccttaa aatagatttt cctgaatacg
    10321 tattggggat ttctaaatgt ttgaatgttt ttttgaatgc aaaatcaatg caaaaaattt
    10381 tgtatttctc tacttttctc tccttgacca aaataaaaaa acgttgattt aacaacgttt
    10441 ttcatacatg tttatctgtc tcaaccatac atatatggag acggcgggag tcgaacccgc
    10501 gtccaaacac atcgccactt aaaaatctac gttcatagtc aactcattta aggattcact
    10561 ttacagcttg ccgagtgaca ggcattctgt atcgctagtc tgatgttctc tttttcaact
    10621 tacagacgga aagttgaaac gtatcccact aaagttagga cccttactcg agcacatggg
    10681 cgatgccgag aggatctacg ctaactgttt ttaggcagct aaagcgtaag agttgttttc
    10741 gtttttagca gttatattta actgtaacgt ttttacgtag acgtaaccta cgaaacgcaa
    10801 ttcaagctcg actgtgcctg tcgaatccgt aacgtccccg ttagctaaca agggttacgt
    10861 gtatttttca tacaaaatta gtatagcata tattgggata aattcaataa aaaagcgaat
    10921 catatcccat tatttatata ataactgccc ctttcttaag aaaaaataag cgattttctt
    10981 aagaaaagag gtagcttcaa aaaaagatct taaatcagat caaaatactt caatgcgtgg
    11041 acaattccgc cttctgtatt tttctttgtg ataaaggtag aaagttcttt taatccgtca
    11101 taggcgttgc ccatagcgat tttgttgtcg caagcttcca acaaggcaaa atcattcgga
    11161 ccatcgccaa atccaaaagt aggaactttt tccagctgaa gttctttttg cagacgtttg
    11221 acacctgccc cttttgagat tcctttcttc actgtatcga tcgagtaagg accattccga
    11281 taaaaagtta gctcaggaaa atgttcgtga tagtattcgt cacctgattg agataagatc
    11341 agcaacatat ttactgtgtt ttctttaaag cctaaaggat caatttccgg aacatctgaa
    11401 tgaatgaagt cgtaagcatt gatcattgtt tggctatgac cggtacacca gattttctga
    11461 tcgttatagt aagaaagttc atgaccgttc aactgtacat gatcatacat tttttgacat
    11521 tcttctgaag aaaatgtttg agaatagatt tcttcgccgt ctactcgaat gaaagaacca
    11581 ttcatggtga ccgcagaagt aatgcccgca tcttccatga tagagtggat ttctgcttct
    11641 gtacgtcctg tagcgatgac ggggagaaca ttgttttctt ttaatgcgtt catagctttt
    11701 gccacttcgg gtgtgatttt tgatttcgca tctagtaatg ttccatcaag atcaaagaaa
    11761 gtgattgcac gataatttga cacaaataaa actccttttt tattggaata agtttatctt
    11821 aacaaagaat gaacaaaaga caaacctttc gtgaagaatt cctgaacata ttggttcagt
    11881 caacaattta gagtatacta taagaagaga atttaggagg gaaagaatga tttttttcag
    11941 tattggtatc ttattcttaa taattggcag tctttttcgg attctgccct caaaaggaaa
    12001 tctgcctttt tatgggtatc attcgcctct tgccgcaaaa acagacgctc attggcggct
    12061 ggctcaaaag acaagcggaa actggttttt tcttatgggc ttactgatgg cattgatcgg
    12121 ctattattta aagacatctg ggcatacgaa ttattttctg attgaaatgc ttctgctagt
    12181 atttccaatt atgcctattt ttattatgac agaaaagaaa ttacagaaat atgacctgga
    12241 aactggaggg aacgataatg aatattttaa tgattgaaga taatcaatct gtttctgaaa
    12301 tgatgcagat gtttttctta aatgaaggtt gggaagcaac tttcaagtat gatggaaaag
    12361 aagggctcga tgccttttta gaaaacccac aacattggga tatgattaca ctagacctga
    12421 atttgcccac actggacggt atgctagtag gaagagagat ccgcaaagtt tcaagtactg
    12481 tgccgattat catgctgaca gctagagatt ctgaaagcga tcaggtgatc ggcttagaga
    12541 tgggggcaga tgattatgtc acaaaacctt tcagtcctct gacgcttatt gctcgaatga
    12601 aagctttgca tcgccgtagt gagttggcag aagcaagaga agaagcagca gtttcagatg
    12661 atcattttga tatcgagact agtcatttca agatgaatac gaaaacacga gaagcgtatc
    12721 tggacaaccg gccaattgaa ggactgacgc ctaaagagtt cgatctattg tttactttag
    12781 caaaaaaacc acgtcaagta ttctctcggg agcagttgct tgaattagtc tgggattatc
    12841 agtactttgg tgatgaacgg acagtggacg cccatatcaa gaaattacga caaaaaatcg
    12901 aaaaggtcgg accacaagtc atccaaactg tttggggagt cggctacaaa ttcgatgatt
    12961 ctggtgttgc ctaatgcgtt atttgtatca gcaactctta gctttttgcg gaatggtcgc
    13021 gttgatctta ttgatcgttg gagtttcttt tacacaactg acaaaacaaa cgattgaaga
    13081 aaacaattac cagcaactgt ttggatatgc tgaatcagtt gaaaagacaa cgcaaaccta
    13141 tgccgatacg ctcccgcagt ttagtcaaga tcaggcgttt caaaatgcat tgctgctgac
    13201 agagcaagta ctgacagaac aaaatgtgaa ctttatattt atcgataaaa acgaacgtgt
    13261 catttatccg actaatacag ctttactgaa ttttacctta acagctgatc aatggaaaaa
    13321 tctgaggaat ggacgtcaag aaaaattcac atcgaataaa aatattttag gacaagcaca
    13381 ggcgacctct tacgcacttg tgccatttaa tttgaatcat gaattctatg gagcacttgt
    13441 agttagtcaa cctgcgcgaa atatcgataa cagcgttcgt ccaattacac tgaacctgtt
    13501 caaaggattt attttttcca gtatcgttgc agttattgcc agctatgctt ttgcagcatt
    13561 ccaagttaag cgtatcaatc ggttaaggaa tgcgacgaaa gaagtgacga atggtaattt
    13621 tgatgtacag ctccccgtac atgacaaaga tgaatttgat gagttagcag atgatttcaa
    13681 taaaatgacg aattcattga aagaatctca agcagaaatt gaagaacaag aaaatcgtcg
    13741 aagacaattt atggcagatg cttctcacga gatgagaaca cctcttacga caatcaacgg
    13801 acttctggaa gggttagaat acaatgcaat ccctgaaaat cagcgagaaa acgcgattaa
    13861 attgatgaaa aatgaaacag agcgtctgat ccgtttggtg aatgaaaact tggattatga
    13921 aaaaatccgt acgaatcaaa tcagtatggt cattaaaaaa ttcaatggaa cagaaacatt
    13981 gaaaaatatt gttgcacagt tagaagctaa agcagaagca gcaggagata cattgacatt
    14041 gaaagccgtt gatgatattg atgtttacgc tgattatgac cgatttgtcc aaatcatggt
    14101 caatattatt caaaacgcga tacagtttac tgaaaacggc caaatcacga ttactttaga
    14161 aaaaggctat cttgaaacga tcatcacgat tgaagatacg ggaattggaa tgtctgaaca
    14221 acaaatgaaa agtatctggg atcgttatta taaagtagat ccttcaagaa aaaatacaaa
    14281 atatggtgaa tcaggattag gtttaccaat cgtgcagcaa cttgtacgct tgcataaagg
    14341 aaaattagaa gtagaaagcg aactaggaaa aggaacaaaa ttcgctgttc gtttgcctga
    14401 cgtagaaatc aaaaaagagt aaaaaacaag cggtataaaa gatcatgaat cgatcttcta
    14461 taccgcttgt ttttactata aaatgattga tttagcccgc gggctctaaa cgatcaatat
    14521 aaggactttg agcaatagta gcatcatgag taaagagtaa gataccttga ttttcttcat
    14581 gagctaaata agaaagcaag tggagtagct cttggatctc caatacacta agttcatcaa
    14641 atgtatcttc aataacaata ttgtttcgct tggaaagcaa ggcatgaagt aattgcagct
    14701 ttactttttc aaaagacgtc aactcagctg ccgcacaatt aagcaaggaa ggagagatat
    14761 caagtttttc ttgataggcc aaacgatctt gtttgatttc tttttccggt acatccaaca
    14821 ggacatggtc tttaagattc agataaggaa acatcggcca atttttttgg atgatcgcaa
    14881 aattatccag ttttgcaaca agaacctctt ttttcaagtg ctccaatatt ttatgctgtt
    14941 tttcgagagg acacaaaata acggtcaagt gttcagcatg aatcaaatat tcaggtttca
    15001 ttacgtccag gcctcctact ttgcaaatga tggataaaaa aagtgaaagg gaatagacta
    15061 agaatgagac agcatccact gagcccaata agagtttggg caaatccctt aagtatatct
    15121 gtgaaaaaaa gatcattttg gaaatcgatg gtaaaaaaag tctgttgatc catcggaatt
    15181 gtaagtccgc ttaccgaagt caagatatca cggatatctg gcagttcagt agtctttaag
    15241 acaagaaaat tgatcctttg aagaagcgct tcataaactg actggaagct tacgatccac
    15301 aataaaacca agatacaaca aagaaagaga ggaaagaaca gctgccagaa aataaaaagg
    15361 aataatttgg ttttagaaag accagtcagg cgccaattga tcaattcctg cttgttttgc
    15421 ctgactgaat agtaaaagaa aaagataaaa aggaaggcaa atagaaaaaa taaactgaat
    15481 atgcctttta tataaaatcg gctagctaat tctaaagccg gaagaagctc tgtagatgca
    15541 gaagcgccaa taaatgaaag ccgatcttgg acttgattgg ctaaagccga ttgcaaatgg
    15601 taaagagtca ggcatgctat caataaaaaa gcagaaaagc ttgaaaataa acaatagagt
    15661 attgtctgct ttttgtaata aattaatcga gcagtagcat agcggattat tttcatactt
    15721 ttcacctcta taaaaagtat agaaaatgaa tatgaataaa gtatgaaatt cctttagtga
    15781 aatcacgaat taaaaccaga aaatattttg taaaaagcaa gaggttgtga agaagctgcc
    15841 ctattctatt accgtagatg caagcttttg aacactgttt attcgaaaag aggagcagcg
    15901 acaataattt tgtcgctgct ccttttaaat ttattttgtc attcggaaga acttgtggtc
    15961 gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga ttgtccgttg
    16021 tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc ttttaatttc
    16081 tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc actattcgta
    16141 tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg tttatcaact
    16201 ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt aatagcttca
    16261 ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt aacaaaatca
    16321 ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg tcctaactga
    16381 atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc atcaacttgt
    16441 ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat catataaggg
    16501 acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt tccaactaat
    16561 tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta aaggacgatg
    16621 actgaattat catacaagcc gctttctttc aaataattga agaattcttg tacggcggta
    16681 tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt attggctaac
    16741 gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga tacagcaatg
    16801 aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg ttggaaaaat
    16861 ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata ataagaagaa
    16921 tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca gaatgtacca
    16981 gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat atcaggtgca
    17041 gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt caagccaaac
    17101 aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac ttgatggaaa
    17161 aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa aggtgtaact
    17221 tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg ctggaagctt
    17281 tctaaatgga tataaatcac attttttcct ttagcgagcc cgaacaaatc gtcatttggt
    17341 gctgcataat gttcttttac ataattttct acttctttca tatcattggg acttgcttct
    17401 gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa agcattcaag
    17461 ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc tggacgatca
    17521 gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga gatcgcaaaa
    17581 gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat cagcagaaca
    17641 gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag tcgaatggca
    17701 ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat cgtaatgaag
    17761 tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa aagaaaataa
    17821 atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc aattccaagt
    17881 aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat gccactttcg
    17941 attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag taaaacgact
    18001 agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat acatttcaag
    18061 ataatcttcc tttctgcact tcttaatcac agaagtgtca tgaaaccatg ttacacagct
    18121 cacttattct catttatttt actagtatga caatatagtg tcaattgaaa ccagaagttt
    18181 atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta agacaagtcc
    18241 tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat gaaagtaaaa
    18301 gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca aaaagaagac
    18361 cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc gcaaaaccaa
    18421 tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt attgagccag
    18481 cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc aaaagaaaaa
    18541 agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa tggagaagga
    18601 gacggaatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt ttcttggtat
    18661 aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga aatctacccg
    18721 gaaatcgtag gaggctatga gaaaattcga tttgaatcag taaatctgcc tgagagccag
    18781 ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg agtaaaattt
    18841 gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa agaagtacgg
    18901 ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt tagttatacc
    18961 ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag tgtcgatcta
    19021 gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt ggatgtcgat
    19081 cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa aagaaaacaa
    19141 tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa gaaaaaagtc
    19201 tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat tttagcagaa
    19261 gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca attccaaaca
    19321 atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc tttatatcgt
    19381 ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct gaaagtgttt
    19441 gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggtttg ggtaaaggga
    19501 ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc cgtccgagag
    19561 gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt cgaatggcga
    19621 gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt ggttcttatc
    19681 catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc tgaaggcgga
    19741 gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat acacacagga
    19801 ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa aagactgata
    19861 gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt taaagaaaca
    19921 ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg agccgatatc
    19981 ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat ggagttgact
    20041 agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg aaatcttggt
    20101 aaaatttctc gtctggctgg cggaacgacg ggttccgtta tgacatttgg ttccttaagc
    20161 agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca aattattcaa
    20221 ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa agaaaaaaat
    20281 agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata cagagcacga
    20341 atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc ttccagaaag
    20401 ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc ctcttgttgc
    20461 tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt cagggaataa
    20521 aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca tacgtggtgg
    20581 ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa ttgcagaaag
    20641 ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt tagcacctca
    20701 agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa tcatacgtat
    20761 aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt ttttatacat
    20821 tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc cgctgtgata
    20881 ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata cagaccatca
    20941 atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc tccgccagtt
    21001 acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc tttgcatagt
    21061 tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg atccatttca
    21121 agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca agcattgacc
    21181 aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc ttgtttggtt
    21241 tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc taattcttta
    21301 ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg ggtgaaaagt
    21361 aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg taaagtgatg
    21421 tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc cgatatcaag
    21481 ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac gatcttgtac
    21541 ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa gataatggca
    21601 ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc tttgtggatc
    21661 aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag acgattgagc
    21721 aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc atgatcctct
    21781 tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt attccattgc
    21841 gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa atcatcaaca
    21901 ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt ttttcctgct
    21961 tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact gatcgctgcc
    22021 atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc cattccctca
    22081 cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaaatgt tttgagccaa
    22141 aagagaaaat gagctaaaat gagggaagat aagcaaagga gcgtggaacg atgacaaacg
    22201 caacagaata tgttcaagca attcaagaaa agctacatca aaaggatcaa gggcaagaag
    22261 aatttttaca agcaatcgat gaattcatgc ccacggttat gtcttttttg gacactcatc
    22321 ccgaatatat cgaaaagaat atcttagaac ttttaacgga accagaaaga gttattcaat
    22381 ttcgagtgcc ttggcaagat gatgaaggaa actggcgagt aaatagaggg taccgtatcc
    22441 aatacaactc agctatcggc ccttataaag gtggattgcg cttccatcct agtgtcaatc
    22501 taagtatctt gaaatttttg gcttttgaac agattttcaa gaatagttta accggtctgc
    22561 caatcggtgg aggaaaaggc ggaagtgact tcgatcctaa aggaaaatct gataatgaga
    22621 tcatgcgttt ttgtcaaagt ttcatgctgg aattgtcaaa acatatcggc ccatcgattg
    22681 atgttcctgc gggagatatc ggtgtaggag ctagagaaat cggctatttg ttcggcgaat
    22741 ataaacgatt gaaaaaatat gatactggtg tgttgacagg taaacctttg gatttttggg
    22801 gaagtaagat acgtacggaa gctactggtt atggattagt gtattatgtc aaacatctat
    22861 tgaacgaaga aaaagattca tttaatcaaa aaacagtttt tgtttccgga agcgggaatg
    22921 tagccattta tgcaatcgaa aaagtgcaag agctaggcgg gaaagttgtt acatgttcag
    22981 attctagcgg gtacatctat gatccagaag gaatagatgt gaaactattg aaagaagtca
    23041 aagaaatcaa tagacaacgt ttgacggaat acgcaaacca acgtccttct gctatttatc
    23101 atgctggaga atctgtttgg acattgaaag aaaaagcgga tatcgctttg ccatgtgcta
    23161 ctcaaaatga aatagacgaa agcttggccg aaatcttagt agaaaatggg atcaaaatcg
    23221 tagcagaagg cgctaatatg ccatgtacag taaaagctgt ttctgttctt catgaagcag
    23281 gcgtgtggta ttgtccagga aaagctgcta atgctggagg agtagcagta tctgctttag
    23341 aaatgagtca gaacgtccag cgattgactt gggaaagaga gcaagtggat gagcagctgg
    23401 atcaaatcat ggaaacgatt taccatacgt gtcgccaaac agcagccgag tacggcgacg
    23461 aaaaaaatct gcttcttgga gcaaatgttg ctggatttga aaaagttgcc aaagcaatgg
    23521 cagcagaagg tttagtttga aaaatgaaac tttcttggaa atattgtcag cattttgttt
    23581 atttgcttga aaaataataa aaaaaaacgt aaagtattac ctgtagagta aacctataag
    23641 gaggagaaca aatggcacac attcattttg actattcaaa agtagcaccg tttgtcaacg
    23701 aacaagagtt agggtacatg caaagccaag taacagcagc ccacaacgaa ttacgggaag
    23761 gtactggcgc aggaagcgac ttccgcggct ggatcgattt accaacgaac tatgacaaag
    23821 aagaatttgc acgtatcaaa gaagcagcta aaaaaatcca atctgattct gaagtattag
    23881 ttgttatcgg tatcggtggt tcttatttag gcgctcgtgc agcgatcgac ttcttgaatc
    23941 acacattctt caatttatta gatagcaaca caagaaaagc accacaaatc ttctttgccg
    24001 gaaattcaat cagctcaaca tatattgctg acttgatcga agtaatcggt gatcgcgact
    24061 tttcagtaaa cgttatttca aaatctggaa caactactga accagcgatt gcgttccgtg
    24121 tattcaaaga gcttcttgtt aagaaatatg gacaagaaga agcaaacaaa cgtatctacg
    24181 ctacaacaga taaagcaaaa ggtgcagtta aagtagaagc agatgcagaa ggttgggaaa
    24241 ctttcgtgat tcctgatgac atcggcggac gtttctctgt attaactgct gttggtttgt
    24301 taccaatcgc tgttagtggt gcagatatcg atgcattgat gcaaggtgcc gctgatgcaa
    24361 gcaaagcata ctcaagcgac aaactagaag aaaacgaagc ttaccaatac gcagcaatgc
    24421 gcaacattct ttaccgtaaa ggcaaagtaa ctgagttgtt gatcaactat gaaccaggaa
    24481 tgcaatactt ctcagaatgg tggaaacaat tgtatggtga atctgaagga aaagaccaaa
    24541 aaggcatcta tccttcaagc gcaaacttct caactgattt gcactcttta ggacaattca
    24601 tccaagaagg acgtcgcaat atttttgaaa cagttgtgaa ggttgaaaaa cctcgtaaat
    24661 caatcacaat tcctgaacaa gcagaagatt tagacggtct aggctacttg caaggaaaag
    24721 aagtggactt cgtaaataca aaagcatttg aaggaacatt gcttgctcat acagacggtg
    24781 acgtgcctaa cttgctagtc aaaattccag aaatggatgc ttatacattg ggttatacaa
    24841 tgtacttctt tgaaattgct gtaggtattt caggttactt gaatggaatc aacccattcg
    24901 accaaccagg tgtagaagct tacaagaaaa acatgttcgc acttctaggc aaaccaggct
    24961 ttgaagatct tgctaaagaa ttgaacgaac gcctgtaaga taagggtttt tcgctagatt
    25021 taaataatca gtgatagaaa catgttcgct ttaaaatgaa aactggtacc catttaggta
    25081 ctcaaacata ctgcatcgtt tattcagaat aaggggctgt gacaagacgt ttgtcacagt
    25141 cccttgtttg tatagaaatc gattgattgc cttgctcttt agtttgtttt aggactttaa
    25201 gaacagtgag gctgacttcg aaggttaatt ctgttttttt gttttctaga atcagtaagc
    25261 aaaaaagcac tcacttcgag tgcttttttg cgatatattc ttagcttaag ataggcctaa
    25321 tttttctttt acagatgagg gtaccttatc gctggtaatt acttttttct ctatgatccg
    25381 cttttcgctt gctttcaact gcaagatcgt tccttcgggg taagcagaac cattggcaga
    25441 gtcaccagaa gtaatgacat cgaaatctaa ctgtcttttt tcaccgtttt cagcataagc
    25501 atctacgttg tatttaaaac ctcttccaat tacttcccca ttatctgctt tgatatcagt
    25561 ttctgcaggc attggagctt tgactaccgc ataataatct ttacctacat aagtagactg
    25621 atagtagtcc caacctttcc atcctataaa agcaatcaat atgataacga tcaaccctaa
    25681 aatttttttc atgaaagatc tcctttttta tttttat
//
LOCUS       DARBEC010000033        25651 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.033, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000033 DARBEC010000000
VERSION     DARBEC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.033"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..2198
                     /locus_tag="SCL80_001297"
     CDS             138..2198
                     /locus_tag="SCL80_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEG1107176.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRREESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            2281..2850
                     /locus_tag="SCL80_001298"
     CDS             2281..2850
                     /locus_tag="SCL80_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1107177.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(3025..3831)
                     /locus_tag="SCL80_001299"
     CDS             complement(3025..3831)
                     /locus_tag="SCL80_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107178.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            3969..5483
                     /locus_tag="SCL80_001300"
     CDS             3969..5483
                     /locus_tag="SCL80_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEG1107179.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            complement(5886..6869)
                     /locus_tag="SCL80_001301"
     CDS             complement(5886..6869)
                     /locus_tag="SCL80_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEG1107180.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(6874..7521)
                     /locus_tag="SCL80_001302"
     CDS             complement(6874..7521)
                     /locus_tag="SCL80_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1107181.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            complement(7606..8238)
                     /locus_tag="SCL80_001303"
     CDS             complement(7606..8238)
                     /locus_tag="SCL80_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1107182.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="SCL80_001304"
     CDS             complement(8254..8805)
                     /gene="coaC"
                     /locus_tag="SCL80_001304"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEG1107183.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            complement(8802..9539)
                     /locus_tag="SCL80_001305"
     CDS             complement(8802..9539)
                     /locus_tag="SCL80_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEG1107184.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            complement(9536..10525)
                     /locus_tag="SCL80_001306"
     CDS             complement(9536..10525)
                     /locus_tag="SCL80_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1107185.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            10783..11325
                     /locus_tag="SCL80_001307"
     CDS             10783..11325
                     /locus_tag="SCL80_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107186.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            11342..12427
                     /locus_tag="SCL80_001308"
     CDS             11342..12427
                     /locus_tag="SCL80_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107187.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            12439..13245
                     /locus_tag="SCL80_001309"
     CDS             12439..13245
                     /locus_tag="SCL80_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107188.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            13246..14073
                     /locus_tag="SCL80_001310"
     CDS             13246..14073
                     /locus_tag="SCL80_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107189.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            14070..15143
                     /locus_tag="SCL80_001311"
     CDS             14070..15143
                     /locus_tag="SCL80_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1107190.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            15571..18267
                     /locus_tag="SCL80_001312"
     CDS             15571..18267
                     /locus_tag="SCL80_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1107191.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            18344..18832
                     /locus_tag="SCL80_001313"
     CDS             18344..18832
                     /locus_tag="SCL80_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1107192.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            18844..19338
                     /locus_tag="SCL80_001314"
     CDS             18844..19338
                     /locus_tag="SCL80_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1107193.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            19359..20168
                     /locus_tag="SCL80_001315"
     CDS             19359..20168
                     /locus_tag="SCL80_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1107194.1"
                     /translation="MYDAFVVALAVFIGIAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            20165..20986
                     /locus_tag="SCL80_001316"
     CDS             20165..20986
                     /locus_tag="SCL80_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1107195.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVLGILLSLLGIA"
     gene            21037..23136
                     /locus_tag="SCL80_001317"
     CDS             21037..23136
                     /locus_tag="SCL80_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEG1107196.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            23133..24251
                     /locus_tag="SCL80_001318"
     CDS             23133..24251
                     /locus_tag="SCL80_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEG1107197.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            24290..25168
                     /locus_tag="SCL80_001319"
     CDS             24290..25168
                     /locus_tag="SCL80_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1107198.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            25359..25511
                     /locus_tag="SCL80_001320"
     CDS             25359..25511
                     /locus_tag="SCL80_001320"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1107199.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
BASE COUNT         8117 a         4457 c         5422 g         7655 t
ORIGIN      
        1 ttttcttgaa acaataaaaa atcaaaaaaa atactctcct aatagtccat cttcttttga
       61 ttttatttat ataatgaaat catcttatga aaattactac ttcatgttta gacaagagta
      121 gggaggacta agaataaatg tgttcaaaaa gacgttcaaa acaactaaat aaacacgctt
      181 gtcgtttaga taaggaacgg tcgtgttctg aaaaacacta cgttaagttt atgatgcgca
      241 cgatgctgat cagtttatct gtaggtgtct atatgcatgt acccgttcag gcaacgacac
      301 aaaccatcaa ggtcgtacgt acctctgttt ctgagaccac tgattcccct atcactatta
      361 ctcatacttt cactgatgca aatggaaaac agctacttcc tgcattgact tcctataata
      421 aagtcagtta tcctccaacg ataaaccact actggttaat tcctaacaaa acattatgga
      481 catcggaaac cggaagcagt gtgccgcttt ctatcttttc cgatatgact gatcctacaa
      541 aaggtgatca gttaaaacag atcactgata ttttgaatca tgctttgaca gatataaagg
      601 acggtggaag cgcacaagtt tcttatgtat atgaaaaaga ttattcacag tttgaaagaa
      661 agaaaatcga tgttctggca gggacaaaag tttcgatatc tgatctggta gaaaggctga
      721 agaacgcaga tggaacacaa ggagatatgt ccaaagtaat ttcaaacaat gggaatatcg
      781 tggatacgac taatacagga acatctacta tttccttgtc ctattttgat ccaattgctt
      841 taaaaacaat gactgcatct gctgtgatcc atgttcgtgc tgatcaaaca gctatcaaag
      901 gaaaagatct tcacttaaga acaggagaga gttacgcccc taaagatctt gttgaatatg
      961 taacaaaaag cgatggaact tcaggaaata aagaagaaat caaaatgaac gggacagtag
     1021 atactacaac tccgggagaa tatcccgttg tattaactta tatagactcg aaaacatcaa
     1081 atgtggcaga aacaacagct gtagtttatg tagaagcatc tgctgcttca tcgactgtca
     1141 tcactcattt tatagatagt caaacaaata aagaacttca agcagaaaca catatgagta
     1201 gtgtaaatcg tattcctgat ttgcgcctac ctgctgaatt ttctgatcga gtgccaaata
     1261 tagatttatc gacttatgaa caaaatggga aagtcacttc tatttctgat tggatgcaag
     1321 caaacggatt agacagaaaa agtcactggt cggagatcct tgaagtactt cactcagaac
     1381 tgatgaataa taggaagcaa tcaggagggg aaattacctt tacttatgtt tatataccag
     1441 acaatcgttc attagaaggt catgatctta ttgttcatcc aaacgaaaaa ataaacaaag
     1501 aagagttgat ccaatcagca acaaattcct taggtaaacc cgtaccgtta aaggatgtat
     1561 cggttacaat caatgaagta ccattggagg atggttattt gaccaaacaa acaggtgatt
     1621 ttacgattat gtatagttat catgacccgt attcactcca agaactccat gctcagtcac
     1681 tattacatgt tttaccatta aacaaacaga tagatcaaaa agaacaaaaa agacgagaag
     1741 aaagcttgtc agaaaaaaaa caacaaatgg ctcatgcagt ttctccaaca gggaaagtaa
     1801 aacaaacaag aagcctagag ttagcaagaa tatctagaaa aatctcagaa tctaataaaa
     1861 cgataaggca agaagctcat ttggccccac aagtttctgg attggaaaat gaaattttag
     1921 agcctaaaat aaaacaacaa tcagaaaaag aatttgttgc taaacagtcg gaagatcaga
     1981 aggaatcaga tgaccagaag gaaacgaaga aaaaacgctt gtcaactatt gattccaccg
     2041 atgagcatct gcagccgcaa gtaccacttc ctagtccggg aacacccgca cctggtggag
     2101 gtattccttc ttctgcttta ggagatttca tgcgaggtat ggcaggaaca tgggtatacg
     2161 ctaatcgaaa tgaatttgac gcagatgact ggttctgatt tatatctcta tctgttatag
     2221 tcaaataagt agctgagtca tatgttattt ggcataatgg agacgaggag ggatagaaaa
     2281 atgttagata aaacgttacc ttacgcagaa atctggatga caagaccact tcaacaaaat
     2341 gttccaatca cgcctttagc ggaaggtttt gaatttcgat tttatcaaaa agaagatgaa
     2401 tctgcttggg cggagattga gacttccgtt ggggaatttc aaacgacaga tgaagcactt
     2461 gagtattttc aacaaaactt tgctccttat cctgaagaac ttgaaagaag gatgtttttt
     2521 gtagagacaa aaaaaggaga aaaagtagca acctgtaccg cttggcgaaa aaaatcaaga
     2581 gagcaagtgt atccggtttt tcattggtta gcagtgaaac cagattacca aggaaaaggg
     2641 ctcgcaaaag cattgactgc cagagtcttg caagaatttc ctaccctcca tacggacgga
     2701 ccaatctatc ttcacacaca gacatggagc catcaggcga ttgctcttta taaaaaaatg
     2761 ggttttactt ttattgctga aaatctagac ggcacaccta atccagatta tgaaaaagtt
     2821 atgcaggttt taagtaaatt agataaataa cgaaatggtg aaataagtcc tcagtattgg
     2881 gataaaaaac gaaatagtca ttcctcatat cttacttttt tgagttaata ctatcggata
     2941 actaatggca tgccatacat tcaaaaaaga aggggcagtg acaaaagtat tgtcactgcc
     3001 ccttctcttg ttttttagca tgttttatcg tttgattttt ttaggaataa aattcttgat
     3061 gaacgtaaat ttttggtaat cgtttcgact atagaggtaa ccaaatacac tgaaaacaac
     3121 tgctccaata aaattaacaa ataaatcctt catagtatcc atgattccca cgtctaagta
     3181 accattttgg atgattgttt tatctgtttt accatctgca ttttgacttt caatgattgt
     3241 ccgagtgata tctggaacat gatagacagt gtttccgtct ttacttaact ccacactatt
     3301 gatttgctgg atgatcgtat ctttttgcat atcgaattgc agtaatttat ctgctgaaaa
     3361 ttccacaaac tcccacataa caccaactgt catcgaaaaa cagaaggaaa caactgctag
     3421 taataaagga gtcaagttca tggtttttgt atttttattg agcaaataaa caagggaaaa
     3481 gccgacaccc ccagctaaaa atccgctgaa tgcatgtaga aaagtatccc acattgaaaa
     3541 atagccataa aaatcactta actctcctaa aactgttgaa ctaaaaataa aagataacaa
     3601 aatgatttct agcatatttg gaatctgtat ctttaatatc ttttccagaa aataagggat
     3661 ttcaaataaa attaatgtca gtaataaaag cattgtattt ccccagtgtt ggttccagac
     3721 ctgatccact agcataataa cgacgatgat acgtaataaa atatagattc cgtttgtgat
     3781 tctacctgca gtttttgtac tgttcgtaaa aaaggttttc aaatgattca taaaataact
     3841 ccttcatgta tatccttaat tatactaaga agcgtgtgtt taccaaagct atatgactta
     3901 aagaaaaaca gagttgatat tgtgaaaaaa atcacttaat atagtataag gagaaaaggg
     3961 ggatacaaat gaaaaaatta gttgctggtt tattaacagc cgttgcactt cttgctatga
     4021 gcgcatgcgg cgcagatcag aaaaatacag cgcagacaag ttctagtacg aaggaatctg
     4081 ccgaagtatc atcaggcgct tctgaacaag tatatacaga tccaaaagaa atgaaagaat
     4141 cttatgatgt cgtgattatt ggagctggtg gtgcagggat ggcagcagct atcgaagcaa
     4201 aagatgctgg gttgaatcca gtaattttgg aaaagatgcc tgttgccgga ggaaacacat
     4261 ctaaatcatc aagcggtatg aatgcttcag aaacaaaatt tcaaaaagaa gaaggaatca
     4321 aagactcgaa tgatgccttc tatgaagaaa cgctaaaagg tggtggagga acgaatgata
     4381 aagaaatgct tcgatacttt gtcgatcact ctgctgatgc ggtcgattgg ctagatcaaa
     4441 acggtatcaa actggacaac ttaacgatca ctggagggat gagtgaaaag aggacacatc
     4501 gtccgtctga tggctcggct attggtggat acttagtgga ggggttattg aataacgtcc
     4561 atgagcggga aatccctatt tttgtcaacg cagatgtgac agacattaag aaaaatgggg
     4621 aaacggtcaa tgaagtgact gtacatgttc aaggagaaga accaaaagag gtcaccggaa
     4681 aagcaatcgt agttaccaca ggcggttttg gtgccagcaa agaatttatt gaaaaatatc
     4741 gtcccgattt agctgattat attacgacaa atcaagaagg atcaactgga gatggtatca
     4801 ccatgattga aaaattaggt ggacaaaccg ttgatatgga taaaatacaa attcatccaa
     4861 ctgtgcagca ggatgagggg gtcttgattg gtgaggttgt tcgcggagaa ggagcaatct
     4921 tggtcgatca agaggggaaa agatttgtca atgaaatgga tacaagggat aaagtatctg
     4981 cagcaattac agcattgcct gaaaaatcag cttatttgat ttttgaccaa ggcgtgcgtg
     5041 atcgtgccac agctattgaa ttttatgacc aaaaaggata tgtcacaact ggaaagacaa
     5101 tggaagaatt agcagaaaaa atcgatttac caaaagaaac actagcaaaa acagttgatg
     5161 gatggaatac ggatgttgcg gagaaaaaag atgctcaatt caaccgtgcc acagccatgg
     5221 aacatgactt atcgtctccg aattattatg cgatcaaaat cgcaccagga atccattaca
     5281 cgatgggcgg tgtgaaaatc aatacgaaca ctgaagtatt agataaagaa aaccagccaa
     5341 ttagggggtt atatgctgct ggtgaagtca caggtggctt acatggaaac aaccggatcg
     5401 gtggaaattc agttgcggag atcattattt ttgggcgaca agcgggcata caagcagctc
     5461 gatttgcttc ggaaaatgaa tgaaatagaa cgtaaataaa aagaggttgt gaaatcagcg
     5521 ttttgagcta ataccgaggg tcagctgatt gaacactgtt tagtgaagag gttgtgaaaa
     5581 agctgccctg catcaagtaa taagaacagc caagtaaaaa cagcttctta tgtttttaac
     5641 ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattcg gaaaaagagc
     5701 gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa acggcggaag
     5761 cagaagaaat tccttcggaa ataagctgaa attcacaaaa atttgaaaag caattttcgt
     5821 gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctttt ttgatcagca
     5881 tgattttatt cagttatctt caaaatgatt tttccgatgt ttttgtttgc ttccatatat
     5941 tcatgcgctt gtttgactgc gctgaattgg aatgtacggt caatgattgg ccgaatcttg
     6001 ccattttcta gaaaaggccc agctttttgc ataaaagttt gtgcgagctc tgctttatac
     6061 tgatcacttc taggtgtcaa taatgtacca agcagttgga cacgtttccc aatcaataca
     6121 ccaaggtcta tctcaggcac gatggtccca cctagcatac caatcaagac ccaacgtcca
     6181 tcaacggcta tagaagctaa attcttttcc cagtaagaag cgccgatgaa atccaaaata
     6241 acatcgactc cccgaccttc agtgattttt cgtacctctt cttgaaagtc ttgtttttta
     6301 tagttgatca attcatctgc accaagttct ttacaaaagg cgagtttttc ttctgatcct
     6361 gcagtaacga tgattttcgc gtgagaaagc tgtctagcta attggatcgc tgctgttccc
     6421 acaccacttg ctcctgcatg gatcaagaca gtttcgcctt cttgtaattt tccaagccag
     6481 taaagtgttt gataagcagt caaaaaaact tctgagattc cagcagcaga aacatagtct
     6541 agcgtatcag ggagaaggat tgcacgatcg gcaggcatca cagcatattc ggcataacca
     6601 cctagattga ctaatccgta tacttttgtc ccaggagtaa aagtggagat atcactttta
     6661 ttctcaacta cctctccagc tacttcgact cccaaaatag ggtagggagg tttcaaagag
     6721 actgattctc ttttcataat atctgttcga ttgacagctg ctgtatggac tttgatcagt
     6781 aattcgcctt gttttggttt aggaacaggc acttcaattt gttctaatac atctacagaa
     6841 ccaggatgct tgattgaaat ggcacgcatg gatctattcc tcctttgccc attcttggaa
     6901 tgctgtattc aactctttgc taccttcaga aagtactaga gtcgtacgag aaatatccgt
     6961 ttcttccact agagcggcaa gtacagcagc aactgtagag cgagtgatcg tttttgatgc
     7021 cttctcatta ggcttagtta attgaatgga agtaacttct tcatcgttag tcaatgtgac
     7081 tggttgaaga atgacaaaat ctaagttagt tcgtttcagc cattcatctg cataatgttt
     7141 agtaatataa taatctgtca tggactcagg ccattttgct ggttcatctg cataaacagc
     7201 actgaccatc agatagcgag agacatgcgc attttcagct gcgatcactg ttttgattgc
     7261 gccatccaag tctacttgca gaagattctt tccttgagag ccggcagcaa aaatcaatac
     7321 atcgatgcct ttgaatgctt cggtcatttt ttccacggac caagttaaat caaaagagac
     7381 atacgaaaca ttctcttttt ttactaatgt ttgggaagca acatctctca cccctgcaaa
     7441 aatttcatgg gaacttggag ctaaatcttt tatcaggtgt cggccaattt gtccgtttgc
     7501 acctacaaca aaaattttca tttggatcat cctttcgacg tcaaatcttt ccattcaata
     7561 gtaacaccaa aagagaaaaa aatcagagat cggctacgat gactatcagt tagtaagaga
     7621 agggtgcttg aagtatctta acaagacatt gcatactgct gcagtcgcaa gacctgcgac
     7681 gattccttca ggtataccac ttgtaaaaat aaccgctaga atagctgaat aaacagcatt
     7741 taaatttgtt ccgatgactt tggcataatc ttgctggaat aaaaagtaga tcatattcat
     7801 cactaaaatc gtattggtca atgaaccgga aagtccagct aagaataaag aaacggaatc
     7861 tgctttttgt ttcattagtt tgcgcagacc tttataaacg aaataaggaa ccaccccaat
     7921 caagatgcga ggaataaacg cgatcagcaa ggctttccag cttccatgtt ctgttccgat
     7981 gactgggata aacggagaaa agacaaaaga caaaggtgtt tggataactg tgctacggat
     8041 catgctgatc agtccaaatg taccgccaag aaaagcacct agtcttggtc ctaaaacgat
     8101 cgaagcaatg attacaggaa tatgcatggt tgttgcattg attggaccga tagggataaa
     8161 acctaaaggc gtaacagcta ataaaatcaa aatagctaaa aacatcgcag ttaatgtaaa
     8221 gttttttgta tttttcataa gtactccttt tttctaagca tttctttcat taagagtcag
     8281 catgacctgt tcgatgatcg tctgaatatc agctaaggca ccttttccaa aatctccaca
     8341 agctaaaaga gattctcttg gttcaatttc ctgatagccg acagatctca atgtttccag
     8401 attacgctga gtgatcgggt tttgatacat attcgtgttc atggcagggg caatcaattt
     8461 tggtgttccc actggaagcg ccattgcgac tgttgaaaga agatcgtctg caatgccgtt
     8521 tgctagcttg ccgataatgt ttgctgtagc aggcgcgatg agaaacaagt cagcttgttt
     8581 tgctagttcg atatgattga ttacctgcgg atttgattct tgcatgacgt ctgtatggac
     8641 tgcgcgcttc gacagagatt gcaaggttaa tggcgtgata aaatgtgtgc tgcttttaga
     8701 cataatcaca tctacctggg cacctaattt gacgaattga cttgtaagat cagcgctttt
     8761 gtaagcagaa atacttccag taacgcctag taagatcttt ttcattttga ttcctccagt
     8821 ctaattttct cagtaatcaa tgcagcaatc tcagatttac tttgagcttc ttccactgtt
     8881 ccatctttag ataagagata gccgtggtgt gtctcgccat ggatttccgt caaatcattt
     8941 gcgaaaataa aatcagtttt gttatttatc agactatttt ttgcgactgc cagaagagat
     9001 tcctttggta catctactaa tagtttgaaa ccaatgattt ttgcttttgg ctgccattca
     9061 cgtagataag aaatgatttt tggtgttttc ttcaaaacga gcacaagatg ctcggtgtta
     9121 gaagagatct ttgaaacagt ggtatctgtc tgttcatttt ccgcaaacca attgtccagt
     9181 tcttcttgcg ttgaagaagt gggtaggttt tttgccagct gttcttctga aaaactaaaa
     9241 gcaggagtaa aatcactgac tgccatactg tggataatcg catcgtagtg ttcttttttc
     9301 gttagttctt ctaattgtaa aaataagtcc agtgcagatt cgatcgtata aatcgttata
     9361 ttggagtgtc ttttcggttt cagtgcttgt ttcgtagtca cgtaatcgat tgtggtatcc
     9421 ggattggcag ctaaataatc agctaatgct tgccctaatc gtcctgtcga atgatttgta
     9481 atagcacgta cttgatcgat tttttcagtc gttcctccag ctgtgattag aatcttcatt
     9541 tatggtcacc tacatttact agtgttcttc cttggtgtgt tccagcaagt agtttagatg
     9601 ctgtttcagg caattgatcc agagtaatct cttggacgac cagttcatct gcaatctgca
     9661 aatccgccat ctgttgccat aaagaaagac gttgatctat tggtacattg acagaatcaa
     9721 ttccaatcaa ctggatgttt cgcagaataa aaggcaagac agtcgcgttt agtttgattc
     9781 ccccagcatt gccacataaa gctactgcac cgttataaga gatcaatgga agaagactgc
     9841 ttagctgctc gccacctact gtgtcgatga catagtcgat ttgctgtttt cctaaagctt
     9901 ttgttgtctc tggcagaaat tcatcaggtc gtacgacttg caaagcacct agacttttta
     9961 gccatgtgac agactcttct tttctggaaa aggcaatgat tgactggaat cctagtttat
    10021 gtaacagggc tatcgctgta ctgcctacac cgccagaagc accagtaacg acaacttttt
    10081 tatcggaggt cataccttga cggattaaag cgttcacaca caacatagca gtaaaccctg
    10141 ctgtaccaag gatcatcgct tgacgcagcg tcatattttt tggtagagga actaaccagt
    10201 ctcctggtac tttttgatat tggctataac cgcctggatg ggtgacgcca agcccaaaac
    10261 cagtagcaag tacaagatct tcttttgaaa aacgtggatt gttactttgg acgacagttc
    10321 cggataaatc aatccctgga gtcatcggat atgttcgtat cacctgacca gactcgctca
    10381 ttgccaatgc atctttataa ttgacatcag aataggcaat ttttaccaag acttcatttt
    10441 cagccaactc agtaaatggc agatcactga ttgctgtatg gaaaacaggt tcctttatta
    10501 cctgaaaaga tcgaaaagcg ttcattcgtg agacactcct tttaaaattt tcttttatca
    10561 taccataaag cctactcttt tattcttgtt cttccgtaaa tttagacaaa aattaggtga
    10621 aaaataaaga atcaaacaac tagaatttat tccaaaagat aaagaaatat tcagcaaaaa
    10681 aaattttaca aagggttgcc tttttcaata aatttcgtat actaatacga gtggtagtaa
    10741 acaggaagtt tactattaga aaacttcgat tcggaggaaa ttatggaaat tggtgagaag
    10801 ttaagaaatt tgcgcatcca aaagaatttg acacaagaag aactaggcga acgcacagat
    10861 ttgtcaaaag ggtatatttc ccagttggaa cgagatttaa gttcaccatc aatggaaaca
    10921 ttcttttcta ttcttgaagt cttaggtgtg actccagaag aattttttca tcaagaagca
    10981 gcgaatctgc aagtggttta ttcaaaagaa gatcatacgg tttatttcga tgaagaaaac
    11041 gggtatgaat tggaatggct cgtggctgat tcgaatgaaa aagaaatgga gcctgtcttg
    11101 ctgacttttg aaggtgcagg agaatataag acgtttgaac cttcattatc tgaaacgttt
    11161 atttttgtat tagaagggga attagagtta agcttaggag agacggtcta tgtggcaaaa
    11221 gcaggacagt cgatctatta tcaagcgaca aaacaacatc aattaaggaa tcatctcaaa
    11281 aaacgaacaa aagtattgat tgttgcaacg caatcatatt tgtaatatag ggggtaaaaa
    11341 cgtggggaaa ccaatcattt catttgatca agtagtgaaa cgctatgatg atgaaacagt
    11401 attaaaaaaa gtaagttttg agatcgaaca aggaaaattt tatacactgc ttggtccttc
    11461 cggctgtggt aaaacaacga tcctacgtat tatagctgga ttcaccgaag ctacagaagg
    11521 ggacatctat tttgaaggga aacggttgaa tgatttgcca gctaataagc gacaagtaaa
    11581 tacagtcttc caagattatg cactttttcc tcatatgaat gtttttgata acgttgcttt
    11641 tggattgaag atcaagaaaa tgtccaaatc agatatcgaa aagaaggtaa aagatgcttt
    11701 gcgtatggtc cagcttcctg gatacgaaaa acgggagatt agtgaaatgt ctggtggtca
    11761 aagacagcgt gtagcgattg cacgagcaat tgtaaatgaa ccgaaagtat tgctattaga
    11821 tgaaccgtta tcggcgcttg atttgaaact acgtacagat atgcagtatg aactgcggga
    11881 tctgcaacaa cgattaggaa tcacgtttat ttttgttact catgaccaag aagaagcgtt
    11941 ggcaatgagt gatgagattt ttgtcatgaa taaagggaaa atcgtccaaa gtggtacacc
    12001 cgtagatatt tatgatgagc caatcaatca tttcgttgct gattttgttg gggagagcaa
    12061 tatcgtggac ggtgtaatga tcgaagataa tctagttgag tttgttggta agcaatttga
    12121 atgtgtcgat ggcgggatgc gtctaaatga accagttgaa gttgttttac gcccagagga
    12181 tttgacgatc acgacaccag ataaaggaaa actagtcgta acggtcgata ctcagctgtt
    12241 tcgtggtgtc cattatgaaa tcatctgtta tgatgaacag caaaatgaat ggatggtcca
    12301 ttcaacgaaa aaagctaaag aaggaagcaa agtaggattg gcctttgagc cagaagatat
    12361 ccatgtcatg cgtttcaacg agtctgaaga agaattcgat gcacgtcttg acagctacga
    12421 agaatagggg ggacaagaat gaaaacaatg cgcaggatct attccgtccc ttatattttg
    12481 tggctcggtt tattcgtgat cgcgcctgta ttaatgatca tttatcaatc tttttttgat
    12541 atgaatgggc aattcacgct gaataattat ctatcttatt ttacgtcggg gacatcgctt
    12601 tctatgacga tcaattctgt atggtatgca tttttgatca cgtttttcac gctgatcatc
    12661 agttacccga ctgcttattt tttaacaaaa ctgaagcaca agcaattatg gctgatgctg
    12721 attattttgc ctacatgggt gaatctgctg cttaaagctt acgcatttat tgggattttc
    12781 agtgtgcacg gcagcgtgaa tcatttcttt gattttatcg ggatcgggcc gaaacagata
    12841 ttattcacag acttcagttt tctgttcgtg gcaacgtata tcgagattcc atttatgatt
    12901 atgccaattt tcaatgcatt ggaagagatg aatccttctt tgatcagtgc tagtagagat
    12961 ttaggcgcga atagctttga gaccttccga cgagtgattt ttccgttatc attaaatggt
    13021 gtgaaaagtg gtgtccaagc agtatttatt ccttctcttt ctttattcat gctgactcgt
    13081 ctgattggcg gaaatcgggt gatcacttta ggaacggcta tcgaacagca tttcttggtt
    13141 acacaaaact gggggatggg ttctacaatc ggtgtgattt taatcattgc tatgttcatc
    13201 gtgatgctcc tgacaggcga gaagaaaaaa ggaggtcgct cataaatgaa aaaatttaaa
    13261 tggtcgtatc tttatttagg gatcgtcttt ttattcttat atgcaccgat cttctatctg
    13321 attttttatt ccttcaatga aggaggaacg atgaacagct ttacaggatt tacttgggaa
    13381 aattatgaag ctgtttttga agatacacgt ttgatcatta tcgtattgaa tacgttcatg
    13441 ctggctttct tatctgcatt gatcgccacg atcatcggga catttggggc tatgggaatc
    13501 tattacacga aaaaacgacg tgcacggaca gcgttgttga gcatgaataa catcttatta
    13561 gtctctcctg atgttattat cggtgctagt ttcttgattt tctttacact actaggattc
    13621 ggattaggtt ttcagagtgt attgctttcc catattgctt tcagcattcc aatcgtcgtc
    13681 ttgatggtct tgccaaaatt gcaggaaatg aacgattcaa tggtcgatgc ggctagagac
    13741 cttggtgcta ataatttcca agtagtcaaa aatatcattt tgccttatct ttctccagga
    13801 atcatagctg gatattttat ggcatttacc tattctcttg atgattttgc ggttacattt
    13861 ttcgtcacag gaaatggctt tacgacgctc tcagtagaga tctattctcg tgcaagacaa
    13921 ggaatcagtt tagaaatcaa tgcgctaagt gcgttagttt tcttgttctc catgatattg
    13981 gtcattggat actactttat tagtaaagaa aaccgtccaa aacggctgaa gaaaaacaga
    14041 agagtacgcg agggggtggc acggctgcga tgaaaaaatt acaatctctg tttttaggta
    14101 ttctcatcat tattgcggtg ctgctgcttg gtgttcgtca acttgaaaaa acaagtggca
    14161 tgagtggagc aaaggtactt accatctata actggggaga ttatatcgat cctagtttga
    14221 tcacgaaatt tgaaaaagaa tatggataca aagtgaatta tgaaacattt gattctaatg
    14281 aagcgatgtt cacaaaaatc cagcaaggtg ggacaaacta cgacatcgct ataccaagtg
    14341 aatatatgat ccaaaaaatg atcaaagaaa aactagtact tccattagat cattcgaaga
    14401 tcaaaggatt aaataatatt gatccacgat ttttagactt ggattttgat ccagataatc
    14461 agtattcgat tccatatttt tggggaactt tagggatcgt atataacgat aaattctttg
    14521 atgcaaatca aattaagcat tgggatgatt tgtggaagcc ggaattaaaa gacagtttga
    14581 tgctgattga cggtgcacga gaagttatgg ggctttcttt gaatagttta ggctattctt
    14641 tgaatagtaa gaatatggca cagttgacag aagcagcaaa taaattgaat catttaacac
    14701 caaatgtcaa agcaatcgtg gcagatgaga tcaaaatgta tatgatcaat gaagaagcct
    14761 ccgtggcagt gacattttct ggcgaagctg ccgatatgat gtgggaaaat gaacatttac
    14821 attatgtgat cccaccagaa ggatccaatc tttggtttga taatatcgtt atgccaaaaa
    14881 ccgtgaaaaa taaagaaggt gcctatgatt tcatcaactt tatgctagaa ccggaaaatg
    14941 cggcacaaaa tgcagagtat attggttatt ctacaccaaa tgaagaagca aaagcgttat
    15001 tgccaaaaga catttcgtcg gacgaacaat tctatccatc tgatgacacg atctcccatc
    15061 ttgaagtcta caaagatcta ggatcgaaat acctaggcat ctataatgac ctattcttag
    15121 aattcaagat gtataggaga taatggcaaa actcagtaag aatggggact tatcaacgtt
    15181 ctaaagagct aaaaatcgtc ctttttctat ctgagttttc caatcttcta ccaaaagcgg
    15241 atgaaacgcc gctttttttg tgttatttta ttttttctac cacgtttttc taccagagaa
    15301 aagtggacaa aaccattgta aaatagggcc cgagacgggc tagtaaatct aagattgaaa
    15361 aagttacaga aggataaagt aattggaaac tatgcaaata tacttaaccc ttctgtaatc
    15421 tattaataaa actaatatat gaatccagtt caatatttgt agctatgaaa aaacataaaa
    15481 atcatattag ataaaaacta aattggtaga taaattattt tgtcagatat ctgaggcaaa
    15541 tattcgacta tttgtgtttc aaagtaaaaa ttggaataca aatatagaat agacatattg
    15601 cagccgatag tttattatgc ttttagacac attaaactgg aggaaaaaat gaaaaagagc
    15661 gctaaagatc gagtttttga tgagctgata cgactaacag ggaatatgtc ttctgtcact
    15721 gccgagaaaa tttcgaagaa tctaggtatc tctcgtcaga atgccagcca ttatttgacg
    15781 agattagtgg aagataaaaa ggtcgagaaa ttaccgggaa agcctgtttt gtggaagcct
    15841 ttagatgaat atgcggttgt agataatacg gagcaaataa acgaagcctt tcattctgta
    15901 gttggtcatg acggaagtct gagagaggtc attcaaaaat gtatagccgc tgtcaaatat
    15961 ccgcctaacg gactcagtgt attaataaat ggagccacgg gtgttggaaa aagtttttta
    16021 gccacaaaaa tttttgaata cgctatccat gagcaaatta ttgaaaaaga tgcaccattt
    16081 gctatcttaa actgtgccga ttatgcggac aatcccgaac tgctttcggc aacactgttt
    16141 ggatacaaga agggggcctt tacgggggct gaaaaggata ccgatggttt attggcaacg
    16201 gccaacaacg gctatctttt tctcgatgag gtccatcgcc tatcgaaaga aaatcaagaa
    16261 aagctctttc tatttattga tactggaaac tatcgtccag tcggtgaaaa tgtaaattgg
    16321 cattctgcta aggttcgttt tattttcgcc acgacggaaa aaggtgaaaa ttatttatta
    16381 gacacctttg atcggcgtat tcaaattagt gttatgttgc caacttttga agatcgcccc
    16441 attcgagaac gattagagct gattcaatta ttttttcaaa atgaagcgga tgttttacaa
    16501 aaagatatta ttgtttcaag agaagccttg tctttaatgt tatctcatcc ctttagtggg
    16561 aatattggta agctgaagaa tatcattaaa atcagttgtg cggatgtgta tagtcgaaca
    16621 caagaagaac cattaacgat tgggaaaaat gagatcttga ttcagttaaa tatgctggaa
    16681 tccgaagtag aaagcttgcc gattgctagt ttattgataa agaaaaatca aaagataaaa
    16741 acggatcgaa tcaaaccgtt tgattatgat tcgattttgg aaaaaattac tcagctgata
    16801 aatgaaggta atttcaatga aaatttttct tcgataaaga atgaagtaca gcatttggtg
    16861 catcacattg ataaggattc ttttttatca atgagtagaa aattgatgat tcagatgttt
    16921 agaaaggtct gggtatccgt tttaggaaaa acgtacggat tgacgacgag tggacctgtt
    16981 gcagaaatta ttagcagatc ctatgtcatt tccccaagct ataagacaga cttaaccggg
    17041 ataaatcgta cattagctgc aaatctacct cggacgactt acttatgtaa tcagttcttg
    17101 gagcatttac caccgctctc agaaaaggac gagaactttc taagactgct tattgtcgtg
    17161 actttatctg attatgtaga tgaatccatc gagctgaagg gcctgttgct tgctcacgga
    17221 gatagtacgg ctagcagcat tcaagcagta gtgaatcaat tatgcggcaa ttatgttttt
    17281 gaagcgttgg atatgccagt agagagtaat gtgtcagaga ttgtcgaatc gacaaggaat
    17341 ttcgtgaaac ggcaagtgaa tacagatagt ctagtgctga tcgtagatat gggctcttta
    17401 aatcaaattt acacacagat caagaacgat ttaaaagggg agttacttct tctgaataat
    17461 ttgacgactt ccattgcact tgatattggc ttgaagatga cgatgaatgc gccatttaaa
    17521 cagattgcgg aggatgcagc agacaagtat tcaatcaatg ttcaatactt tgaaggcttt
    17581 tctcaaagta gcaacattat catttcctgt atgtcgggtt tggggatttc ggacaaattg
    17641 aaagatgttt ttcgtcatca tttatcggat gaatccatcg aagttttcac gaaggactac
    17701 agagaactac gtgaattaat cgaccaaaat gatgaaactt attttgacaa gacaaaattg
    17761 gtcatcacga cttcggatct tccacgttcc ttttcaattc ctaatattaa tgtttatgac
    17821 atcttagacg gtgatggagc agccaatttg gagcatattt tgagtcctga aatgaagccg
    17881 gcagaattta accgcttgat tcaggagcta gtaaaattct tttcgttgga aggaattgcg
    17941 gaccgtttga actttctgaa cccaacgatc gtgatgagtg aagtggaaac agtcatttct
    18001 aaatatgaaa attactatca tttcacttta aatggccgtg taaaactaaa tctctatatg
    18061 catacggcat tgatgatgga acgtttattc ttggctcgga cagaaaaatc tgaagaaccg
    18121 aattatgatt tgaaaccaga tgaagatgaa ttttattatg tttctagaag tatttttcaa
    18181 tcaatggaga tgaaatataa ttttaaagtc aatgcttatg agctttcgct gctctatgaa
    18241 ttattaggac cgtatattca aaaataaagt gtctaaaaaa gtgtctaatc tgtttttttt
    18301 aggttagaca ctttttttaa tgtgaattaa cgtgattaaa ccaatgtttt taaaagttgg
    18361 cacaggaatt gcttatataa aagtgaacag aaagaaagga attaaccata tgaagaaaat
    18421 tctaatagcg actcacggtc acttagcttc aggctttctt agcagtattc aattactgac
    18481 ggggaaggtt caagagatta cggtgattaa tgcttatgtg gatgagtgtg attttgaaca
    18541 ggagttgaat gtatttgtaa accaggtgaa cgagcaggat cagatttttg tttttaccga
    18601 tttatttggc ggaagtgtca atcagaagat taccaaaacc tttttggaga aagagattgc
    18661 tgtaactatt ttgtctggct ttaatctgcc aattctgctc gaaatagtat tggcaaccaa
    18721 ggaattaaca aaggaggata ttcaggaact ggtaaataaa tgccaacagg aattaaagat
    18781 gaccattctt gatgaggtac atgtagtaaa tgaagaggaa gatttttttt aggaggaaga
    18841 aaaatgatta aagcagtgcg aattgatgaa cgattaattc atggacaagt agcgatgact
    18901 tggacaaaga gtctaaattt aaccgggtta gtagtagcaa gcgatgaggc ggctaccaac
    18961 gatattcaaa agatgacatt gaagatggct gcaccatcga atgtcaaagt cattattaaa
    19021 acagttgatg acgcgattca tttgttaaaa gatccgcggg cgaaggacat gcgcttgatg
    19081 gttttagtcc ctaatgtcaa agatgcggta aaagttgcga aagctttccc aacggaaatc
    19141 gaattaatga acgtggggaa tgctgggaaa atgacaccgc catcggaaga taagaaaacc
    19201 ttatcaaagg aagtgatgtt aacgacgtca gaaattcaag acttaaaaga attagttgaa
    19261 ctttatccag ataccgtttt tcagcctaca ccggcgatgg aaaaacgttc agctatctct
    19321 attttgagtg atttttagaa aaaggaagga gaaaaattat gtatgatgca tttgtagtcg
    19381 ctttggccgt ttttattgga attgctggac atgaaatgtt cgggatgtcc atgctgagtc
    19441 gtccgattgt tgttgctccg ttagcaggag cgttgatggg ggatttgcag acggggttga
    19501 ttgtcggggc gtcactagaa gccatcttta tgggtgtcgt caatatcgga atctctagta
    19561 cggcggagcc ttctttggct gctgggctgg ctacagcgtt cgcaatcaaa atgggaggaa
    19621 acgtggatgc gattattccc attgtctttc cactagcagt attaggatta cagttaatga
    19681 atatgatttt ttcttttgta tgtggaccga tggctgctaa atttttcaaa tttgcaaaat
    19741 ctggtgatca aaaaggaatc atcaatctgc acttctttat gtggtttgtc cactatgcgc
    19801 tttatgctct tattccattc tttgccgttt tatttggagc agatgttgtc aaaagcgtct
    19861 tggataatat tccagaggtc atcatgaacg gattaacagt cgctggaaat ctgctgccgg
    19921 cagttgggat ggcgatgctg ttacggatgc tttggagtaa aaatattgct gtgtggtttt
    19981 tctttggggc cgttgttatg gcgtatctcg agttaccatt gattgcaatt gccgcagttg
    20041 gaacgattat tgcaattgtt atagcccaaa gagatctaca actaaaaaat atatctgttc
    20101 atgcagttca aagcgatgct gctgtaaatg caacgcttga ccaagaagag gaggatttct
    20161 ttcaatgaaa ttagctgatg gaatttctaa agatgagaaa aaattggttc ggaagatgtt
    20221 ttggcgctcg atgactatgt atgcttcggt gaatccggtg acgatgggcg gcggtggttt
    20281 cgcctattct atgatgccct tcattaatca tttctacaaa agtgaagaag atcgagctgc
    20341 ggcgctggaa cgtcatacac agtatttttc gacgaccatt ccttgcgcgt cctttgtcat
    20401 ggggattgcg gcatcgatgg agaaggaaaa ttctgaaaaa gctggttttg acgtggaaag
    20461 tatcaacgcg atcaaaacga gtttgatggg accattggct ggaattggcg attcattgtt
    20521 ctggggagtt tggcgtgtcg ttaccgcggg actagcgatc aacttagcca atgccggaaa
    20581 tattttggca ccaattatct ttttagttat gttcaatata cctaattact tgatgcgata
    20641 ctatggcggc gttttaggct attcattagg ctctaaatat attgaaaagc tgtatgcgaa
    20701 cgggttaatt gaaattctga caaaagctgc tagtatcttg ggtctgatca tggtaggtgc
    20761 aatgacgatc agtatggtca aattcaatac cgttttgagc ttttcaatga agggcgaaga
    20821 actaatgaat gtccaagaaa tcctcgatag tatttttaaa ggaattgtac cgttattggt
    20881 aacattgagc tgtttcaaat tactagataa aaaagtcggc gtcatcaaaa ttatttttgg
    20941 aatggtcgtc ttgggaattt tattgtcatt acttggaatc gcatagtagg aagctgcagc
    21001 tactaggctg cagttttttt agcaaggagg gaaaagatga ctattaatga agtaatttat
    21061 tcacccaaac acccattttt taatgctgag ttaaacgacg gaaatgtgac tacttcaaac
    21121 gaatacagct actatcggaa tattcctgat gtaaagggat tgaccttcga tgtaagtaat
    21181 caaaagacat tagcaaacct tgatgtttgc gggacgattc aaacgttgac cttttatcga
    21241 gaaaatttac taactgagga aaaaccaggt gtttgggtca ataaacagct ctctcaaacc
    21301 aattcgttat ccttaaaggt tgaagtaaac gggattgtct accatttgtc agaatcggat
    21361 catcgtattg aagtcgatgt gattcaagac tgtttgccta gatatattca tcattattcg
    21421 acgtttaagg tcattgtagt atcctttgct cccatactgg ctaataagcg tttaagtatg
    21481 cttgttcaac aggtttacat tgtgaatgaa acacacgaaa gccttcaagt ttcagtgaaa
    21541 gatgtgcctt cgtatcaaga gaagtattcg gatcagcaaa atgtgttgat ttctcaaaaa
    21601 ggaaataagg acacgatcgc acaacaggaa gtggcaagct tcgccattgc ttttgtggac
    21661 ccaaatgctt ttgaagaagt aatgactttt caggaatctg atatcgaaaa atggctgaag
    21721 gaaacattga actattttgc tcagctattt gggcagctgt caatgccaaa cgatagagtt
    21781 gtccatctct atcgtcgtgc actttatcaa agcttttcta gtttcggtat gaatcgacag
    21841 ggacaggtcg tcggcagcaa ttggggcagc tatcctgcta ctaatcgaat ttggaataag
    21901 gatatgtatt acgcatcatt gccattcacc atgtttgaac cggagttatg tcaaaaaacg
    21961 gttctatggt ttgatcaata tggcgttaag tttcctggtt caaaatttac aggaggtatc
    22021 aatcattctc tgagtaattc gttatccagt gcgttactgg ctagtcttta ttttgaacat
    22081 actggggata caaaattctt tgagcggtgt ccgcaggttc tactgaatgc ttcaagaatt
    22141 attgaggaca tattagctca acgtcggtca ggggaaccaa tgctttttcc atctgtttgg
    22201 ttatcagacg cctttgcact tggaaagtat cataccggca gcaatctttg tctttggaag
    22261 gcctgtattg gacttgcaga aatttttgaa gccctagaac aactgagtct tagcaagcga
    22321 tacaaaaaca ttgcctgtaa actaaaggaa tcaatttgta aacaaatgac tatcgatggt
    22381 ccttttggtg agcaattttt ggagggaatc ggtgatgagg agaaaacaac ctattcagtg
    22441 aagcactatc aaaaaccgat tcttgagcaa gggttaatct ttttaagtga tgtgattgtg
    22501 gatggaaaaa ttaatttatt gatgcatgat ggagaagaat cagacactac gttgatgcct
    22561 ttttatcaat ttttagataa taaagatcat ttatatcaga acaccatggc cttttctgct
    22621 agttctttca atccaaccta tagcgaggaa atcaaaggca tcacttgggg attagaatcc
    22681 ggcgccactt ttcctggctt tatcactgtt ttaatgagcg agctgcacaa ccctcaagct
    22741 ttcgtaaccc gattagaaga actaatttgt ttggcagatt tagacggttc gtggtggtgg
    22801 tggccgtatc gcttagaggc tcaacaagga gaagtcgtgc gagactttgg ctgcggcaaa
    22861 tgcggctggg catcgggtct ttttgtcagc ctaatgatca gtcaatattt cggattgaaa
    22921 ttcaaaaaag gagtattgca aattgatcct gttgataatt taacgttttc ttggaagaat
    22981 ttgaagttcg gtcaagcctt catttcgatt gaatgtacgc aatccgagtt gagtatttca
    23041 aatttgtcag aaaagccatt gaaaataagt gatacgaaaa aaattcttca cgtagaaaag
    23101 ggaaaaacga ttcacattca aaggagaaaa agatgagtat actaaaaaat attacccaat
    23161 ttaaaaaaat ccagagtcga gcaatcactc ccgaaaatcc aacaggcgag aaaggtaaag
    23221 ctgcgatgtt gccaagcgag ctaggaccaa gccgcaaagg tcgtggcgca attccgctga
    23281 cttctggtga agaaactgtg attgcggata ttactggaac gggaataatt cgtcatatgt
    23341 ggatgacgat tcgtgatcga acagaatttg gcagttttgt tttacgtgat ttaatcttaa
    23401 gaatttattg ggatgggtcg gatagccctg ctgttgaaag tcctttaggg gatttctttt
    23461 gtaatgggtt tggagagcgt tgcgaggtga actcgttgcc gatagtggtt aatcctacag
    23521 ggggctttaa ttcctatttc gaaatgcctt tccaaacatc cgctaaaatt acgatcgaga
    23581 atttacatcc gaaggatatg tcctcatttt tttatacgat aaattacgct ttggtggacg
    23641 aattggaaga aaatagccta tattttcacg caaattggca tcgggaacgc attaccaaat
    23701 tacaggagga ttataagtta attgatcgat taaagggtca aggctattac gtgggtactt
    23761 accttgcgct tacctgccta gaacgttatt ggtggggcga aggcgagttt aaattctaca
    23821 ttgatgggga tagtgagtat ccgaccgtaa cctcaacagg ttcagaagat tattttggcg
    23881 gggcgtgggc ctttcatgag tacgatgagt tcggacgtcc acatgcgaaa aactattcaa
    23941 cagccttttt agggtatcct taccaatcaa atagagatgc cacaagagat cgttttgaaa
    24001 cgggaaaatt gaatgccgtt catgcctttg gtgacgacgc aatgccacga catggtcttt
    24061 atcgttggca tcttcaagac cccatctgtt ttaccgaaga tttatcggtc actttacaac
    24121 aaatcggcaa cgatgatgtt cgcttatttg agcggcaaga cgatgttgct agtgtcgctt
    24181 actggtattc aacagaagct tcaggaaata ctaaacgtca tttcactgat cgagaacgct
    24241 tgccgcgtta atgaagtgtt tttcacatat tcgaagggag gaacctatga tgatatatgg
    24301 ggcaattgaa gctggaggga ctaaatttgt ctgtgcagtc ggtactgagg aattaacgat
    24361 tttggaaaga gtcagttttc ccacaacaac tccagttgaa acaatgaagc aggtcattga
    24421 tttttttaaa ccttttaagg aacaattggc aggcatcggg gtcggctgct ttggtccaat
    24481 tgatattcaa tgcaaatcaa cgagtttcgg tcatatcaca tcaacaccaa aacttgcttg
    24541 gcaaaatttc aattttgtag gaacgctaaa gcaatatttc aatattccga ttgaatggac
    24601 aacagatgtc aatgcggctt gctatggtga atatgttgct ggttctggca aagggttagc
    24661 aggtgttgca tacttcactg tgggaacagg tatcggaggt ggtgccttgt tcaatgggac
    24721 tttcgtggaa gggttcagtc atccggaaat ggggcatatg ttgatcaagc gacatccaaa
    24781 ggacaatttt tcaggaaact gtcccttcca ccaagattgt ttagagggga tggcagcagg
    24841 accagcgatt gaaaaacggt tgggtgtaaa aggccagaat ttattggctg acgattcgtt
    24901 ttggcagatt gaagcctttt accttgccca atgtgcatac aatacaacac tgatgttttc
    24961 gccagataga attatttttg gcggtggtgt aatgaaacaa gaacacatga agaaaaaagt
    25021 tcaggataaa tttgttgagc ttataaatgg atatgttgaa ataccaccaa ttgatagcta
    25081 cattattacg cccgaattag gtgataatgc cggaataatc ggcggattag cattggccag
    25141 aaaagcggta agaaataagc aaccataatt aatgaatgtt tcattgatac tcatgtcaaa
    25201 agtatttaac cgagtaatca aatgatcaaa tgaagttgga taaagcgaaa atgaattcaa
    25261 ataaaaaggc tctaagtcaa atcgtgttgt tttcattaaa tcactgaaag gaaatgcttc
    25321 tgcgtttcct tttttctttt tgttgtgcgc tgtatacctt gaggatattc actggtggcg
    25381 tacacttaaa tagtaatata ttgtgttact ttatgtctgt ggtaatagtt atagttgctt
    25441 accaatgtat gttcattttt cgaaatgttg acttcataat ccttggctta atgtttgaat
    25501 ttttttggtg atagattttt tcagccgtat aaaataaatc gtaattctat tatagagagt
    25561 tgtgattata aaaagatagc aagaaaaaaa aagaaagttt ctatacattt taaatatttt
    25621 attcctattt ctattgctaa aaaagtagaa a
//
LOCUS       DARBEC010000034        25625 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.034, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000034 DARBEC010000000
VERSION     DARBEC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25625)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25625)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25625
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.034"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            133..3660
                     /locus_tag="SCL80_001321"
     CDS             133..3660
                     /locus_tag="SCL80_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEG1107200.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3657..7379
                     /gene="addA"
                     /locus_tag="SCL80_001322"
     CDS             3657..7379
                     /gene="addA"
                     /locus_tag="SCL80_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEG1107201.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7448..7789
                     /locus_tag="SCL80_001323"
     CDS             7448..7789
                     /locus_tag="SCL80_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107202.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8098..9141
                     /gene="pheS"
                     /locus_tag="SCL80_001324"
     CDS             8098..9141
                     /gene="pheS"
                     /locus_tag="SCL80_001324"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEG1107203.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9146..11566
                     /locus_tag="SCL80_001325"
     CDS             9146..11566
                     /locus_tag="SCL80_001325"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEG1107204.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCL80_001326"
     CDS             complement(11736..12746)
                     /gene="trpS"
                     /locus_tag="SCL80_001326"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEG1107205.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            13000..13737
                     /locus_tag="SCL80_001327"
     CDS             13000..13737
                     /locus_tag="SCL80_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1107206.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13751..14566
                     /locus_tag="SCL80_001328"
     CDS             13751..14566
                     /locus_tag="SCL80_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEG1107207.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14579..15226
                     /locus_tag="SCL80_001329"
     CDS             14579..15226
                     /locus_tag="SCL80_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1107208.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15242..15898
                     /locus_tag="SCL80_001330"
     CDS             15242..15898
                     /locus_tag="SCL80_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1107209.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16060..16881
                     /gene="racE"
                     /locus_tag="SCL80_001331"
     CDS             16060..16881
                     /gene="racE"
                     /locus_tag="SCL80_001331"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEG1107210.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16884..18239
                     /gene="rph"
                     /locus_tag="SCL80_001332"
     CDS             16884..18239
                     /gene="rph"
                     /locus_tag="SCL80_001332"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEG1107211.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKKQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18240..18758
                     /locus_tag="SCL80_001333"
     CDS             18240..18758
                     /locus_tag="SCL80_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1107212.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18865..19365
                     /locus_tag="SCL80_001334"
     CDS             18865..19365
                     /locus_tag="SCL80_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1107213.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19645..20565)
                     /locus_tag="SCL80_001335"
     CDS             complement(19645..20565)
                     /locus_tag="SCL80_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEG1107214.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20730..21743
                     /locus_tag="SCL80_001336"
     CDS             20730..21743
                     /locus_tag="SCL80_001336"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEG1107215.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCL80_001337"
     CDS             complement(21853..22608)
                     /gene="fabI"
                     /locus_tag="SCL80_001337"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEG1107216.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22828..23268
                     /gene="fabZ"
                     /locus_tag="SCL80_001338"
     CDS             22828..23268
                     /gene="fabZ"
                     /locus_tag="SCL80_001338"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1107217.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23383..24144)
                     /locus_tag="SCL80_001339"
     CDS             complement(23383..24144)
                     /locus_tag="SCL80_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1107218.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24333..25427
                     /locus_tag="SCL80_001340"
     CDS             24333..25427
                     /locus_tag="SCL80_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEG1107219.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
     gene            complement(25513..>25625)
                     /locus_tag="SCL80_001341"
     CDS             complement(25513..>25625)
                     /locus_tag="SCL80_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1107220.1"
                     /translation="SVSNQRNHIPRKSLNYRTPIEIFLSYVQEAFYSSLI"
BASE COUNT         8484 a         4439 c         5407 g         7295 t
ORIGIN      
        1 tttttgcgta tacttaaaaa agtagaaata tcagaacctt aaaaggatat aatttgaaga
       61 atatagaaca aatgttcagt attttttgat ataataaagg agcaccgtat catgtgttga
      121 aggaggaaaa gcatgtcttt acaatttatt cttggaaacg ggaaaaaaga tcaccgtcaa
      181 gtcttgatcg acgaagcatc cgcttggctc gaccaggatg caagtaacca agtatttttc
      241 cttgttccca actacagcaa attcgaacag gaacaagaaa ttttagcgga gatgcgaaga
      301 agaagcggaa aaaaaacttt cagcacaaca aacgttcaag tatttagttt ttatcgtctg
      361 gcttggtatt tgctgcaaca gacaactctt cttacaggaa atgaattatc agaatcagga
      421 tctgcaatga tcttgcgaca gattttagaa aaaaatgagg aagaattaac aatctttcgc
      481 ggagaaatca ataaaagagg gtttattcgt caattacaag aactctacag tgaattgcgt
      541 acaggaaata ttcaaccaga agacctgatg cttttatttg gtgattcacc taatgcagag
      601 gaccagcaac ttaaaatgaa agacttgaag ctgatttttt cagcttatga cagcgaattg
      661 actcaacgtg cactaacaaa tgaagatgca ctatctatac tagcaaatta tatgcagaca
      721 caagatttca acaacgtcaa atttattgtc agtggttttt ctcgaatcaa tggtcaagag
      781 tatcaattac tgcaggcaat gatggaaaag gggcaattgg ttgtcgattt attgctggat
      841 cgtccttata cagcggatct tccagaagta cttacgctct ttcatgagac tggaaagctt
      901 tatttccgtc tttttcaaag tgcaagagaa cggaaagtcc ctatattagt ggaccgattt
      961 gcaccagagc agactcgtcc gacagagctt caactgctgc aaacaatgtg ggaagagaca
     1021 tctagccaaa agaaagcggc agtacacaaa atagggggac aagagcatct ccatttgtgg
     1081 gaagctgaaa atccaactga agaagtccga caattagctg ttgaaatcag acgactagtc
     1141 agtgaagaaa actaccgtta tcgtgatatc caagtgctga caaaaaatat gtctttatat
     1201 ggaaatatcc ttcggccgat ctttcaagaa atggcaattc ctttttatat cgatgaagaa
     1261 caaatgatgg aaaatcatcc gctgatcgaa tggatcaaca gtctgttcgc tcttgaccgc
     1321 tattcttatc ggctaagtga tattatgcgt tttttcaaaa ctgaattatt tctgtcgacg
     1381 gaagcagaaa ccgaacttga tgaatggaaa caagagcgag atgaattcag gaaaaaactg
     1441 gacatcactg aaaatgtagc acttgcttac aattatcaag gaacttattg gactcgtgaa
     1501 aaagactggc agtttgtggc gtatgatttt gaagcagaac agattgaaga cgtacagtat
     1561 ttggaagaac agtcaaatac gattagacga ttatttcaga aagctgtacc agtatttttg
     1621 aaaaaaataa agaaaagcaa aacaggacaa gaggccgcaa atgtttttta tcaatttttg
     1681 atccaaagtg gcgtacagca acaattattg ttttggcgta atcaggaagt agacagagga
     1741 aacttagaag cggcaagaaa ccatgagcag acatggggag cgctgattga tctgttggat
     1801 gaatatgtag taatttatgg tgaatcggct tttgactggt cactgttcca agaaatcatt
     1861 acaagtggtc tagaaaatct aagttatggt aaaatcccta ccgcaatcga ccaagtgcga
     1921 atcaatcgtt tggaattagt tcgaccaaat caagctgccg tcacttttgc attagggttg
     1981 aatgatcaag tgtttccaga ccgaaaagag acaaaaggat tgttgtccag tgaagaacga
     2041 gaagtattaa atcaggcctt gccagatgga cagtttttat tcgacccatc aaaggaaagt
     2101 atctcttttg agcctttcca agcctatctt gtttttcttt ccggaacaga tcgattatat
     2161 cttagttatg cacaaagcta tgacacagat ggttcgctga agatgtctcc ttatttgcgt
     2221 agaatcacag aatatttatc tgttcccgtc gagagaaaag agcatttgac gatcgaaagc
     2281 gaccctaatt gttatgtagg gacttacaga agcggaatca atacagtcaa taggatttat
     2341 cgattagctt tagatgaaaa aaaacctgta ccaaagcact ggcaaaagct caaagaatta
     2401 gtactgaatt cgtcatggcg aaattttgct ttacagattt ttgaaagtca ggaacattta
     2461 aatgttcctg tttcgatgcc agaaaaaatg gcggaagagc tttatggaaa agagatttat
     2521 acttcaattt cacgtatgga gaatttttat aattgtgaat acaaatactt catgcaattt
     2581 ggtttgaaat tgaaagaacg aactgtttat ggtctaactc cagcagcaac tggtgatttt
     2641 tatcatgaat cattggatcg tttcttcaag ctactgtttt caaaccaatt atccttagtg
     2701 aatatgtccg atgcgcagcg tagagacttt actgaaaaag tgctgcagga agtattcgga
     2761 gaacttcggt ttgagatatt ggatagttct gcaagaatga actatatccg ctaccagtta
     2821 ggacagacga ttcaaaaagt gacatgggct ttgcaaaaac agagcaaaaa tacaggcatg
     2881 catcctcttc agacagagat tctatttggt cagattgctg gagcaaaagg tatcacaggt
     2941 ctggaattac cgttgagtaa tggtggaaaa ctccatgttc gagggaaaat cgatcgaatc
     3001 gatgtagctt ctgaacaaga agatacttgg ctaagcgtcg ttgattacaa atctagcgga
     3061 cgttcattcg atgtcacaga agcttattat ggcatggcta tgcaattatt gacgtatctg
     3121 gacgtagcat tgatggatgc ggtacgtttg actggaaagg cggctgttaa accagcagga
     3181 tcatattatt tacatgttca taatcctgta ttgacggaga cagatgatgt agagaagaat
     3241 acattgaaaa aatttagtta tgatggcgtt tttgtcgatg atcccgctct tttagaagta
     3301 ctagatcatt cactggaagc aaaagaacaa tcactgatct atccagtgaa aaaaaatgcg
     3361 aaagatgaat accaaaaagg tagtttcagt aaagacaaat ttttcacgga agaagaactg
     3421 caactattca tggcgcataa tcgggaaaat atgcggttag ctggagatgc gattacttcc
     3481 ggagaaatca aactaaatcc agcttataaa gataaacagc ggattgcctg cgaattttgt
     3541 ccattcagaa gcatctgtac gtttgatgtg atgctgaaag aaaataatta ccaccgtctg
     3601 gagaaaatag ataaggaaga agcactgcgt agaatattga aaaggagtga agaagagtga
     3661 atcaaattcc tttaaaaccg caaaatgaac gattcacaga tgatcagtgg caagcgattt
     3721 ttgatcaagg agataatcta ttagtctctg cgtctgccgg atcaggtaaa actacagtgc
     3781 ttgttcgaag agtgattgaa aaactaaaaa tgggttttga tatcgatgaa ttattgattg
     3841 tgacatttac tgaagctgcc gcaagagaaa tgaaagaaag aattcaagag gcattacaag
     3901 aatccgtcaa tagcgaaagt gaccctgtac gtagacagca ttttactaag cagttagttt
     3961 tattgcccac agcgaatatc tcaaccttac atgctttttg cctgacagtt attcgacgtt
     4021 attattactt aattgatatt gatccagtct ttcgcatgtt aacagatgaa acagaaacga
     4081 tcttgatgaa ggaggatgtg tgggacgagc tgagagaagc actttatgca gaaaatgatg
     4141 aacgattttt ccaattaaca atgaattttt ctaatgatcg ttcagatgac ggcttgacga
     4201 atctggtgtt ttctctttat gagtttgccc gtgccaaccc tgacccccaa aaatggctag
     4261 aacagttaag cgataattat cgtttgcctg aggggctagc gaaatctcga ttatatcaaa
     4321 aacaaatccg tcctttagtt ttggcagata tataccaatg tgtacagtta tatgaacaga
     4381 tgactcagtt agcacaaggt gaaggattag aaaaaatgca tgagcaagta gctggagaac
     4441 aacagcagat caagaatatc tatgaagcgt tctcgcaaga tcggttagaa gaagcttatg
     4501 ctggtttgga acaactaact ttttcaactt ttaagagcag tcggaaagca gaattgaaag
     4561 aaatttccaa tgaagtcaag ggcatgaggg ataaagcaaa aaaactgatt cagcagataa
     4621 gtaaaagtta ttttcctgtt tccccaagcc aaatggaaga attaacggac aaagcactac
     4681 cgttagtcga agaaatgaca aaagtcacgc aaagcttcat ggacggtttc agtatgcgta
     4741 aacgggaaaa aggtgtgctg gatttcaatg atctggaaca tttagcgttg cagattctca
     4801 cagaaaaaac gaaagatgta tggctgccaa gtgaagcttc aaaacattat cgaaaaaaat
     4861 tcaaagaagt aatggtagat gaatatcaag acgtgaacca attacaagaa gccattttat
     4921 actggctaag agaacccgat gatacaaaag gaaatatgtt catggttggg gatgtcaaac
     4981 aatccatcta ttctttccgt ttagcagatc ctagtttatt tatcgggaaa tacgagaatt
     5041 tctcaaagaa agaaggcgga cgtcgaatcg ttttggctga gaacttccgc tctagaaaag
     5101 aagtcctttc ttttacgaat cttatttttg aacaactaat ggatccagca gtgggtcaaa
     5161 tcaattatga cgaagcagcc aaactgatcc aaggattttc agactttcca gagaatgaac
     5221 agtttgagcc ggaaatcatg atctatgaaa aagaacagga agaatcagag attgagattc
     5281 cgacggatga tatactggaa gacaaaacag aaggcgaact ttttatgaca ggactaaaaa
     5341 ttcgtcaatt aattgattcc tcctttatga tttatgataa aaaatcaaaa aaaagtcgtc
     5401 cgatagagta caaagacatt gttctcttga cacctacaaa gaagaacaac ttaacgattt
     5461 tagagatttt caaaacattg gatattccat tagagatgaa tgatgctcaa aactattttc
     5521 aagcgacaga aattcgcacg atgatctcgc tgctacaatt aatcgataat ccctatcaag
     5581 atattccgct tgcggctgta ttgcgctcac cgatcgtggg tctgattgaa ccagaattag
     5641 ccagcattcg attagctaac cgtgcacata cttactatga tgctgtgctt gcttatcaag
     5701 catcaaacga agatgaatta gcagctaagc tggaacattt tggcaagcaa ctagagcatt
     5761 ggcgagaact tgctcgccgt tcttcgatca ctgacttgtt atgggatatc tattatgaga
     5821 cgggatatct agagtatgtg gtcggtctgc cagcaggggc acagagacaa gccaatcttt
     5881 atgcattggt tgatcgtgcc aaagcttacg agcaaagttc tttcagagga ttataccagt
     5941 tcgtgcgttt cattgaaaaa atgcaagaaa aagataaaga cttagcagaa ccagtcattt
     6001 caatagaaga taatgctgtt cgcgttatga cgatccatgc aagtaaaggc ttggaattcc
     6061 cagttgtatt tttgttagat atgacaaagg agttcaactt acaagacttg cgcaatcggt
     6121 atgcttttga agaaaaactt ggagcaggaa ttcgctatat ggatcctgaa acacgagtgt
     6181 tatatgatac gttaccgttt caagccatca aattggcaaa gcaaaacaaa ttgttgtcgg
     6241 aagaaatgcg taaattgtat gtaggattga ctcgggcaga acaaaagctt ttcattgtcg
     6301 gttcctataa aaataaagag caaatgatcc aaacgtggtc tgaagcagct gatcatgagg
     6361 agttagtttt tgatcctgcg ctacgtttga agggcagaag cagcttgatg aattggattg
     6421 gttacggatt gatccgtcat cctgaaatgc agaaatactt agaggaagaa atttcgactt
     6481 ctcttcttca acattcaaat gcacagtttt ctatttcatg gatgaatcag caatcgatca
     6541 ttgaacaaag acagttgctt gctgaaaaag aactggttaa cttagatcag cagatgaaag
     6601 aagatgaaac tttattagca gattcgttac aaaaacgtct ggcatatgaa tatccttatc
     6661 aagcttcttc acaaaccaca agttatcaat ctgtatctga aatcaaacga ttattcgaag
     6721 atccagatga tacacaagaa tcacgtttga ctctggaaag cagtcagaac aaagcagcat
     6781 cccgtcaatt tcgttatacg caagaacaac tagcagaacc aaaatttttg caaaaagatc
     6841 ggcaagtgtc agcagctgct gttggaactg ctacgcatgc tttgttacaa ttactgccat
     6901 tagagatgcc aactacagag agtatccatc aaaaattaca ggaattggta aggaaacggt
     6961 tagtagacga aaaagtcgcg aaaaaagtag atgtttcttc tattatatgg ttctttcaaa
     7021 cagaacttgg tcaacagctg attgccaaca aagaaaacgt aaaacgagaa caaccttttt
     7081 ccatgctgct tcctgctgac gaagtatttc aagactatcc aaatcaagaa gatgaactat
     7141 taatccacgg aatcgtcgat ggatacctag aagaaaaaga tcatctcaat atctatgatt
     7201 ttaaaacaga ttttatttta catccggatg atccagcaga aatagatgca atcgtccaaa
     7261 aatatcaagg acagctaaga ctttaccaac aagcaatgtc agaagctttg aataagccag
     7321 tagaaaatgt tttcctaatt ttgcttcgag tgaaacaaat tataaacatt aacaaataat
     7381 tataattgag caaacttgac ttatgttttg taaggattat gctatactct gggaagaggt
     7441 gagtatgatg gagctaacac agacagaatt cgttttatgc cctaaatttg aaaaaacatt
     7501 ctctatttta ggaaaaaaat ggaacggatt gattatcgat gtactactcg aaaacggtcc
     7561 gcaacgcttt gttgagcttg cggcaaaaat cccgatggtc agtgatcgag tattagtcga
     7621 acgcttgaag gaattagaaa aagaaggaat agtcactcga aactttgcct gtagcgaaaa
     7681 gagccgttca gaatatctct tgactgaaaa aggagcagac ttgcaaaaag cgatgcgtca
     7741 aatacaagtt tggagcgaaa aatggatcac agaggaagaa tgcggttgat tttgtaaaaa
     7801 aaatcttgta aactatagtc catacgaata agtaaagaac gatgacgaaa aagagtagtt
     7861 tggctaaacc agttcaggga gatctgtcgc agactgaaag cagatcctgt gtttgaaaaa
     7921 tgaagttcac tttccgagtt cgtctgataa aatcagaccg gcaattgtcg ttaacgaagt
     7981 aagagtgcgg tgtaagtcat ttatccgcaa attaggatgg taacgcgaag acctcgttcc
     8041 ttaacaggaa tgaggtcttt ttgttttgta tttggaattt aaaggaggaa caagcacatg
     8101 tctctacaag cacaattaga gagcttgaaa gaagcaacgc tcgtcaaaat cacagcggca
     8161 caagatttaa atgaattaaa tcaaatacgt gttgaaacgt taggaaaaaa aggaccgatc
     8221 acagaagtat tacgtgggat gaaagatcta agtccagaag agcgaccaaa agtcggaagt
     8281 ttcgcaaatg agatccgaga tcttttgaca gaagagatcg agcgaaaaaa acaagtatta
     8341 gaagaagaag cgttaaatgc agctttggca aaagaaacaa tagacgtgac actacctggt
     8401 agaaaagtaa aacatggtac acgtcatatc gtgacacaaa tcatggaaga aattgaagat
     8461 atttttgtgg gtatgggcta tcagatcgta gaaggatatg aagtagaatc tgatcattac
     8521 aatttcgaac ggatgaatct gccaaaagat catccagcaa gggacatgca agatactttc
     8581 tatatttcag acgagatatt gattcggaca catacttcac cagtccaagc acggacaatg
     8641 gaaaaacatg atttctctaa gggtgctcta cgaatgatct cgccagggaa agtcttccgc
     8701 agagatacag atgatgcgac ccacagtcat cagttccatc aaattgaagg gctagttgtt
     8761 gataaaaaca tcacaatggg cgaccttaaa ggaacattag aagtcgtaat gaaaaaaatg
     8821 tttggggaag atcgtgaaat tcgtttgcgt ccaagttatt tcccatttac ggaaccatca
     8881 gtagaagtag atgtcagctg tttcaaatgt ggtggtgccg gttgtaacgt atgtaaatac
     8941 actggatgga tcgaaatttt aggagctggc atggtacacc cgaatgtgtt gaagatgtca
     9001 ggaatcgatc cagaagaata ttcaggcttt gctttcggcc ttggaccaga ccgcgtagct
     9061 atgttgcggt atggagtgaa tgatatccgt aatttctatc aaaatgactt acgtttctta
     9121 agtcagttca agggggagta agaaaatgtt agtttcatat aaatggctca atcgttatgt
     9181 agatttatcg aatgtaacac caaaagaatt agcggataag atgtctgtga caggaatcga
     9241 agtagaggga gtaacggttc cagaagaagg cttgaaaaaa atcgttgtcg gtgaagtcaa
     9301 ggaatgcgta ccacatccag attctgatca cctttctatt tgtcaagtcg atattggcga
     9361 agacgaactg tctcaaatcg tttgtggagc gcctaatgtg aaggttggaa tcaaagtgat
     9421 tgttgcactt cctaactcac gtatcgctgg taatgtaaaa atcaaaaaag gaaaaatgcg
     9481 tggccaagta tcaaatggca tgatttgctc attacaagaa attggctatt cagatagtgt
     9541 tataccgaaa gaatacgctg aaggtatcta ttatatgcca caagaagctg taaatggcga
     9601 tccagttttt ccttatttgg atatggatga ttcgattatt gaattatcta tcacaccgaa
     9661 tcgtgcggat gcattgtcaa tgcgtggcgt agcttatgaa gttggggcaa tttatcgtca
     9721 gactcctaca ttcgatgacg aaaagctggt agaatcctca cgtcctgcta gcgataaaat
     9781 tcaagtggaa gttgaagata aagaagcagt tcctgcttat caaatcagaa tcattgaagg
     9841 agtaagaatc cagccaagtc ctcaatggct gcaaaatctt ttgatgaacg aaggcattcg
     9901 tccaatcaac aatgtagtag atgtgacgaa ctatattttg cttttattcg gacaaccact
     9961 acatgcattt gactacgaca aattgaaatc aaaagagatc tttgtccgcc gtgcaaaaga
    10021 aaatgaaaca ttagtaacac tagatggaga atctcgggaa ttgacaactg acaatctcgt
    10081 cattactaac ggcaaagaac cagttgcttt agctggtgtc atgggagggt tagactcaga
    10141 gatcacgaat gaaacaacaa cagttgcact agaatctgct ttatttgatc caatttcgat
    10201 tcgtcgtaca gcaaaacaat ttaatttgcg tagtgaatcc tctgcacgtt ttgaaaaagg
    10261 aatcaaccat gcgactgttg gagaagccag tgaggttgct gcagcaatga tcgtttctct
    10321 tgcgggtggg gaagttctac aaggcgcggt caaaggctca gagatcaaag cagaagacgt
    10381 atcagtgaag atttcacttg aacgcatcaa tcaatattta ggaacatctc ttgtcgttaa
    10441 agaagtaaat gagatttttg aagcacttgg atttagctat gaagaaaagg agtatgttta
    10501 ttccgtagta gtaccaccaa gacgctggga tatccaaatc gaagcagata ttatcgaaga
    10561 agttgctcgt atttatggat acgaccagtt gccatctaca cttccaagtg gagaaacggt
    10621 tgctggaagc ttgtcaaaag aacaagaaac tactcgtaaa attcgtacaa tctttgaagg
    10681 aagcggattg agcgaagcaa tcagttatgc attgacaaca gaagaaaaat caagacagtt
    10741 tgttataaat gaaagtaata ttacacgttt ggactggcca atgagcgaag aacgttctgt
    10801 attacgaatg aatttgatca gcggactatt agatgatatc agttataaca ctgcccgaaa
    10861 aaatacagag attgctttat atgaaattgg tcgggtgttc ttccaagaaa atgatccacg
    10921 tacacatctg ccgcaagaag taaaacatgc agcaatcgct atttctggta tttgggaaga
    10981 aaaagattgg cagacgaaag ctcaaaatgt agactttttc accatcaagg gtcttttaga
    11041 aaacttattt gaacaactag gcatcactga agatgtccat tatcaaacag tgacagaaat
    11101 gaaggaaatg catcctggaa gaactgctgc gatttacctt ggcgaaaaat ttattggttt
    11161 tgtcggtcaa gtgcatccaa cgattacaaa aagttatgat attccagaaa catatgttgc
    11221 tgagtttgat ttatctgcta tagtggaagc tgctcaaaaa gggatcgttt tcgaagcagt
    11281 gacaaaattc ccagcagtca gccgggacat cgctttgctt gtaaaagaaa ctgtgacaaa
    11341 tcaggaatta acagaagtta tccgttctgc tgccggaaga tttttaacag atatccaatt
    11401 atttgatgtc tatcaaggag aaaacattga aaaaggacat aaatccatgg cgtatagcct
    11461 gacattcgtc aacccagaag caacattgac agatgaagaa atcaataaag caatggaaaa
    11521 agtcgaaaaa gcgttggtag aaaacctaga ggcaagtatt cgctaagaag gaaaatatag
    11581 taagtaacga tcaagtagct tttgaagacc atacatttga aaaaaagatg tgacacaact
    11641 tcgtcacatc tctttttttg attttaaaaa aggaatagca cagagacgag ataaaaaaac
    11701 aggcctttac agcctgcttt tttagagtaa atgaattaat ggaagtaatt gattcccatt
    11761 gctgatttta cttcagaaag tgtttgtgca gctactttag cagcttcttc actaccttta
    11821 cgtaagattt ccataacagc tgaaggatct ttttccaatt cttctcggcg cgcacggatc
    11881 ggtgcaaatt cagcttccaa tacatcaatg aggtatcgtt tgatctttac atcaccaagt
    11941 ccgccgcggc ggtaatgagc tttcatttct tcaattgctt ctttatctgt tccgaataca
    12001 tccaagtagg tgaagaccat attcccttct acttgtccag gatcttgtac atggatgtgg
    12061 tttggatcag tatacatact catgactttt ttctgtaaga catcggcagg atcggagata
    12121 taaataccgt ttcctaacga tttactcatt ttgccatttc catcgattcc aggtaatcta
    12181 cccattcctt ttggtgggaa tacgcctttt ggttccacca agacttcacc atatgtatga
    12241 ttgaagcttt gtacgatttc ctgtgtctgc tctagcattg gtttttgatc ctcaccaaca
    12301 ggtacaagat tcgctttgaa tgcagtgata tctgctgctt gagaaacagg atagataaag
    12361 aatcctgtag gcacgctttc accgaatttt ttttgttcga tttctgtttt gacagtcgga
    12421 ttgcgccgaa cgcgtcctac gctgactaag ttcaaataat acatggttaa ttcagctaat
    12481 tccggaattt gtgattggat aaataaagta gattttgcag gatctaatcc cacggctaaa
    12541 tagtctaaag caacttgcaa gacatttgat gaaaccttct caggattttt agcattatct
    12601 gtcagtgctt gcatgtcagc aatcatgaca aacagttggt tgtcttcatc tgcctgcata
    12661 gccactcggg tttttagaga accgacgtaa tgccctaaat gcagcttccc agttggtcga
    12721 tctcccgtta aaataatatt tttcaaaaaa gacacctctt tacgatttgt aagtctgagc
    12781 acttaatatc ttaccgtttt tcctgaaaag aaacaacagt tcctaaggaa aaaacaagga
    12841 aagaggataa aataactaat agattagaaa tggaagaaat atttatggga gaataaacgt
    12901 tctaagaaac tttacaaatt actaaactat cagatataat gtattttatt atacatcttt
    12961 aaaagaaatc ttactaaata ataatgggag tgtaattcta tggcaatgat taaatttgat
    13021 cacgtggata aatattatgg caagttccac gcattaaaaa atatcaatct cgaattcgag
    13081 aaaggagaag ttgtcgttgt cattggtcct tctggttcag gaaagagcac gatgcttcgc
    13141 tgtatcaatg gattagagac gatctcttca ggtaagctgc tgattaatga tactgacttg
    13201 catgataaaa agacaaaact gacagaagtc cgaaaaaaca tcggcatggt tttccaacat
    13261 ttcaatttgt acccaaataa aactgttctg gaaaacatca aattagcacc aatcaaagtg
    13321 ttgaagcaag accaggcaac tgcagtcaaa aacgcggaga aatttttgaa aactgtcaat
    13381 atgttagata agaaagattc ttatccttcc atgttatccg ggggacaaca gcaacgggtg
    13441 gcaatcgcca gaggtttagc gatgaatcct gagatgctgc tgtttgatga acctacttct
    13501 gctttggatc cggaaatgat tggtgatgta ttagacgtta tgaaaaaatt ggctcatgac
    13561 ggtatgtcaa tgattgtcgt gacacatgaa atgggctttg ccaaagaagt ggcggatcgt
    13621 gttatcttca tggctgatgg agaagtattg gaagacagca gaaatgtgag agactttttc
    13681 gaaaatccaa atgaagtccg agcacagcaa tttatcagta aagttatcaa tcattgatag
    13741 gaggaaaaag atgcgcaggt taaaaaaaat acgcctgatt ttccttttag gcattgcttt
    13801 tcttgcacta gccggatgta aaggaaatgg gttggcaaat gaagatattt tagagcgaag
    13861 caaatctacc aatgaaatca tctggggtgt caagtatgat actcgattat ttggaatgat
    13921 ggatattgag agcagaactg tacaaggatt tgatgtagat attgcaaaag caatcactaa
    13981 aaagatcctt ggtgacaatg gaaaaacaga atttgttgaa gtcacttcta aaactcggat
    14041 tccattattg aaaaacggaa atatcgatgc aattattgca acgatgacga tcacagatga
    14101 acgaaaaaaa caggttgact tttcagatgt ctatttcgat gctggccaag ctttgttggt
    14161 aaagaaagga agccaaatca aaagtgtaga tgatttgaat gcttctacaa cggttctagc
    14221 agtcaaaggt tctacatcgg ctgcgaatat ccgacagcat gcaccagatg caaaaatttt
    14281 agaattagaa aactatgcag aagcatttac tgctttacaa tctggtcagg gagacgcgat
    14341 gactacggat aatgcgattt tgttagggat tgcagatgaa aatccagagt atgagttagt
    14401 tggtggaacg tttactaatg agccttacgg gattgcgatc aacaaagggc aagaaaactt
    14461 cttgaaagct gttaaccaag cgctagagga aatgcatgca gatggcacat acgataaaat
    14521 ctatcaaaaa tggtttccaa atgaaactga aggaaaagta gagtaagggg gagaggcaat
    14581 gagcgaattg atacaaactt atggaccaat ctttgtcgac gggttcaaga tcacattgtt
    14641 ttcgagtgtc ttagcacttg tgtttagttt gattatcgga acgttaatgg caatcttcca
    14701 attatcaaag aataaagtag tgagcgcctt agcaaaggca tatgtagaat tttttagaaa
    14761 cattccatta ttaatcatcg tgatgttttt ttatgttgtc gtaccgattt attggtttaa
    14821 tctagatgga ttccaagcag gtacgattgg attaacgatc tatacatctg cttttattgc
    14881 tgaaacagta cgttcaggga ttcaaaccgt accaaaaggt caaacagaag caggactatc
    14941 ttctggtttt acctattcag aaatcatgcg ttatattgtt ttaccgcaag cattcaaaat
    15001 cgttatcccg ccattaggca accaatttat caacctcgta aaaaactcat ctattttagc
    15061 aatcgtagct ggtttagatt tgatgtatca aggagacttg atcgctagtg aaacattcaa
    15121 taccttcgat acgtatatca tcgttgggtt attttactta gttatcacgc taccgttatc
    15181 ttacttaatg gtttatcttg agaaaaaatg ggcggttcgt tcatagaagg aggggaaata
    15241 catggatttt agcggcgctt tttcatggat gaatatccgt tttttattgg aaggattaaa
    15301 ggttactgtt gaagtttcat tgttttcgat catttttagt ttcttgatcg gtggtctgac
    15361 aggtgtgatt cgttttgcga atatcccttt cttttccaaa atccttggtg tagtgattga
    15421 tattatacgg aatcttccat tattattgat cattttcttc acttactttg ccttacctca
    15481 aatcggcatt cagatgaaca tcttctggtc cgctgtcgca gcattaacga tttttgagtc
    15541 agctatgttg tcggaaattt tccgagctgg actcaatgct gttccaaaag gacagatgga
    15601 agcaggatta tcaacgggac tgacttatgt agaatcgatg cgaacgatcg tgttaccgca
    15661 agcatttaaa tcaatgatcc cggcaattgt gagccaattg atttcactga tcaaagatac
    15721 ttcattagca gtaatcattt ctttacctga actttctcat caggcacgta ttatttatgg
    15781 acagaacacc aattatgtgc ttccgatgtt catcatcatg acacttatgt acttcgtggt
    15841 atgttacgcg ctttctctgc tttccggtta cttggaaaag agacggtaca gttattaata
    15901 gatgattctt ttagcggttg tggcaaacta aagtcacaac cgcttttcct ctttccattc
    15961 gaaaaaaaga gatttcgatt gttttaagca agtaagagca cttgaaaatt cacgctaaaa
    16021 agtacacaat agggaggata gaatttattg aaaaaaagga tgatacgatt gacagataat
    16081 cgccctatcg gatttattga ttcaggtgtc ggcggcttga ctgtagtaaa agaagccctg
    16141 aaacaattac cgaatgaaaa tattttattt gtaggagaca cagcacgctg cccatatggc
    16201 cctagacccg cggaacaggt aatccagtat acttgggaaa tgacggatta tctggtggag
    16261 caaggaatca agatgctggt gatcgcctgc aataccgcaa ctgcggtggc tttagaagaa
    16321 atcaaagctg ctctttctat tccagtcatc ggtgtgatcc ttcccggtac tagagcggca
    16381 gtaaaaaaaa cacaaaataa acaagttggc attatcggta cgattggtac ggtaaaaagt
    16441 caagcttatg aaaaagcact gaaagagaaa gtaccagaat tgactgtgac aagtcttgct
    16501 tgtcccaaat ttgtttcagt tgtcgaaagt aatgaatacc attcatcggt ggcgaaaaaa
    16561 attgtggcag aaacattagc tcctttaacc actaaaaaaa tcgatacatt gattttggga
    16621 tgcacccatt atccattatt acgccccatc attcaaaatg taatgggaga aaatgttcaa
    16681 ctgatcgatt ctggagcaga aacagtaggt gaagtatcta tgctgttaga ttatttcaat
    16741 ttgagcaatt caccgcaaaa tggtcggaca ttatgccagt tttatacaac tggctctgcc
    16801 aaacttttcg aggaaatagc tgaagactgg cttggaatcg gacacttaaa tgtagaacat
    16861 atcgaattgg gaggaaaata aggatgcgtc atgacgggag aaaagcccaa gagcttcgca
    16921 aactggagct tcaaacaaat gttttcaaat atccagaagg atcggtagtg atccgttttg
    16981 gtgatacaac agttatttgt tctgcaacaa tagaaaattc tgttccccct tttttgagag
    17041 aaacaggaac aggatgggtg acagcagaat atagtatgct gcctcgagca acgagtacga
    17101 gaaatcgtag agaaagcagc aaagggaaac tttctggtag aacaatggaa atccagcgtc
    17161 ttattggtcg ttccttgcgt gcagtagtag acttggaaaa attaggagaa cgtagtatca
    17221 tcgtagactg cgatgtgatc caagcggatg gcggtactag aacagcaagt atcactgggg
    17281 catttgtggc attgaaatta gccattgaaa aacttttaag agaaaaagaa ttgtcagaag
    17341 atccaataaa agaacatctt gctgcagtta gcgtaggaat cttaccagat gggacttgcg
    17401 tcacagattt ggattaccaa gaagattccg cagcacttgt agacatgaat cttgtaatga
    17461 cagaatctgg aaaattcgtg gagattcaag gaactggaga agaagcaacg tttgatggtg
    17521 aacagttgaa tgaaatgctc ttttttggaa aaaacgcgat tgaagatttg atcaaagagc
    17581 aaaaacatgc tttgctaaca gagtttgctc aaaatgatga gcggatagaa gaaacaaaaa
    17641 caatcatcat tgcgacaaga aatccaggaa aagcagaaga gtttcgaaat atgttcaaag
    17701 aagcgggata tcatgtaaaa acattacttg actatcctga attgccagat gtggaagaaa
    17761 ctggcagcac atttgaagaa aatgcccgat taaaagcaga aacgattgca caattgcttg
    17821 atcagccagt cttagcagat gattcaggtt tgaaagtgga tgctttaggc ggtatgccag
    17881 gaatctattc tgcacgtttt gcaggggaac aaaaaagtga tgcggggaat aacgccaaat
    17941 tattatatga attgacagat gtgccggatg aaaaaagaac agcacaattc cattgtacgt
    18001 tagtattcgc ggcacccaaa aaagacagcc ttgtagtaga agcggaatgg cccggtcgag
    18061 tagcccgtat accatctggc gaaaatggct ttgggtatga tcctttattt atcccagaag
    18121 gtaagaaaca aacagcagca gaattatcga gcgaagaaaa aaacaagatc agtcatcggg
    18181 ctcaagcaat gaaaaaattg agtgccgagt ggaaacaatg gctggaagga gagagataga
    18241 tgcggtattt agtagtaagt gataatcatg gagacagaga tatcctcgtt gacttagtag
    18301 atcgttatcg ttctgaagtg gatatctttt tccattgtgg tgattcggaa ttagaagctt
    18361 ctgacccatt atgggataca ttccaagtgg ttcaaggtaa ttgtgattat ggaccaggat
    18421 tcgaacagaa aaaagtgatc caaactggtc aggatacagt attcatgact cacggtcatt
    18481 tatcgaatgt ccgatttggt ttgacacagt tagcaatcga agctcgagct gcaactgccg
    18541 atatggtgtt ttttggtcat acccatcaaa taggctgtga gatggatcag aaaatcctgt
    18601 ttttgaatcc tggtagcatt tctcagccta gaggcccgat ccaaatccca tcatatgctc
    18661 ttatcgaaag tttagatgac caaatttctg ttcaatacta taaccgtgcc catcaggcag
    18721 ttgaagagtt ggccttttct ttttcgagag agcgctgaat aaaaaaactc taatttttaa
    18781 taaaaataag ctttatttta tgaattttgc atccggtttc ggtaaaatag tagtgttgaa
    18841 tgaaatagaa atggaggcca tcagatgatt ggacctatag taagagaaat gctgctggaa
    18901 aatcaggaaa cgtttttgat tcctgcagaa aatgtggcaa atgttatgta tcatcatcca
    18961 ttgtcccatg gtttacttgt gttatcgaag gtcggttatt ccaaaattcc tgtattagga
    19021 aaagatgatc actttgtagg actagtaagt ttatcagatg tggtcaataa aatgattgac
    19081 ttgcaaacaa tcagcatgga cccgttagaa ggactaacgg ttgctgatgt gacagaaaca
    19141 gatgttccga caattgatga aaattgggaa ctagaagaag tactccattt gttagtcgat
    19201 tcgccttttt taccggttgt cacagaagat cggattttca aaggaatcat tacaagaaaa
    19261 gagctgctta aagccgtaaa cttcatggtg catgaattag aacgtcgtaa tatattgtct
    19321 ccgaaaatcg aagaattaaa agaacaaatc gatatcgtga gctaaagaac gagaaactaa
    19381 aagataatca aaaaataagt agaaaaaaca gcgtaccagt tttgacttta tcttgaaaaa
    19441 gaggctggga caaaagcgtt taactccaag aaataagaag gaattcacga aaattgcttt
    19501 tcaaattttt gtgaatttca gcttatttcc gaaggagttg cttctgcttc cgccgtttat
    19561 acgtgtttag agcgtgggac aaaagtgttc tttacttttg tcccacgctc tttttagtga
    19621 gattcgttgc atcgtttgtc caaattattt tccgacagca attggactgt tcggaatcgt
    19681 gaaaccagaa gctgtcaatt ctttgacata ggagctcaag aactcttctt ttaaggcata
    19741 ttgttttccg ttcaatgcgt agcagaccgt acgaatcgca aaattgctgt ttccaagatc
    19801 cactaatcca aagatagaag gttcagtttg gagctcttct tggtattttt cagctaatgt
    19861 acgatttact cgatcaatga tctcatagat tttgtcatat ccttcttcag gaacaatccg
    19921 aatatctaaa agtacttgca tatttgcacg agagagattg ctgatcgtgg tgatgtttcg
    19981 attcgggatg aaatggacag taccatccgt tgctttcagc tgaagggtac ggataccaac
    20041 agatacgacg gttccttcaa tcgttaagtt tgctagcttg atgtagtccc caacatccat
    20101 ctgttgttcc atgataataa aaaatccggt aataacatca ctcatgaacc cttgggcacc
    20161 aagaccgatt gccacacctg caattccggc accagccaaa agtgatccga tcggcacacc
    20221 gatagcagat aaaattgcat agataaaaaa gaaaccaagg gtataatgaa aaatagtatt
    20281 taaaagacta tgtaaagtct ttaagcggct ctcgtttaga atggcttttt tagaatactg
    20341 attaaaagac ttatcaatca gatattttcc cacacggtga agaatccaaa aaaggacgat
    20401 caaaaaaatc agatataaac ttttttggat aaaggcagcc aaaatggcgt cccaatcaat
    20461 cccgtcccaa aaccgctgaa acgcatttac tttgctgaca gcttgatctg tcaatgtctc
    20521 gccagaagag tctgtcgtac cggcagttgc taataatgaa aacataaaat cctcttttct
    20581 aaaatttgat tctttctgta ttgtaccaag aagttttcaa aaaaggaaat agcaaaaata
    20641 actataatga aggaaaaaag ctgaaaataa aatgacaaat aatatggata atgataaaat
    20701 gtggagaaaa taacgaaggt ggtaagtata tgcaaatcga ttggaacaat ctaggttttt
    20761 cttatatcaa aactccttgg cgttttattg ccaaatggaa agacggacaa tgggaagaag
    20821 gagaattgac agaagataat tacctgacga ttcatgaagg ctctccagcg cttcattatg
    20881 gtcagcaatg ttttgaaggt ctaaaagctt atcgtcgaaa agacggcaag gtcaatctct
    20941 ttcgacctga acaaaatagt cgacggatga atcaaagtgc tagacgattg ctgatgccaa
    21001 tggtccctga aaagatgttc gttgatgctg tcaagcaagt ggtaaaagca aatgaagatt
    21061 ttgtgccgcc ctatggaaca ggcgctacat tatacttgcg ccctttactg attggggtcg
    21121 gtgaaaacat tggtgtccat ccagctccag aatatttatt tgtcgtcttc tgtacgccag
    21181 taggagcgta tttcaaaggt ggactaaaac caacgaattt tattgtatct gattacgatc
    21241 gtgctgctcc taaggggact ggtgcagcaa aagtcggcgg aaattacgca gcgagtttac
    21301 ttccaggaac agaagcaaaa gaactggatt actcagattg tgtttatctt gatccagcca
    21361 cacatacgaa aatcgaagaa gtaggtgcag caaatttctt tggaattaca aaagacggaa
    21421 aattcatcac tccgcaatcg ccctctatct taccaagtat cacgaaatat tccttgttgc
    21481 agttagctga agaacgacta ggactgcaag caattgaagg agatattttc attgatgatc
    21541 tagatcaatt tgcagaagct ggtgcatgtg gtacagcggc tgttatttct ccaatcggtg
    21601 ggatttatca caataacaat cttcatgtat tttatagtga aacggaagta ggaccagtaa
    21661 caaaacggtt gtatgatgaa ttgacaggta ttcaatttgg agacatagaa gcaccagaag
    21721 gctggattga agtcgtggaa taatttcaaa gtaatgagtt gaaaaaaacg taaaaagaaa
    21781 aaatcaggaa gagactttaa aaagtttttt cctgattttt atatgtttta ctttaaagaa
    21841 tgcaacaaaa gtttatgaaa gatggacacc tttgtcaacg tagataatat caccgatgat
    21901 tccgctggaa agtgggctga ttaagaaagc acaagcatta ccgacttctt cgatcgtcac
    21961 accttgctga tcaggcgtac gagattcaga taactggatc agtttttgat agtctttaac
    22021 acctgtgaca gctaatgttt tgattgcccc agctgagatt ccattgaccc gaattccttt
    22081 aggcgagaac tcagctgcca aataacggat agcagcttca agagaagctt tcgcaatacc
    22141 catcatgtta taattcggga ttgcgcgttc tgaacctaga taagtcatgc taacgatccc
    22201 gctgtttggt gcaagaaacg gttgtgcgta ttttgataca gcaattaaag agtagctgct
    22261 gatatcttga gctaattgat agccgtctct ggaaatatca gacacatttc cggacaattc
    22321 ttctttgttg gcgtaagcaa ctgcatggac aaggccgtgg atctccccag catattcacc
    22381 gatggcattc attgcttttt cgatgctttc gtcgctagaa acatcacatt ctacagtgaa
    22441 tgcttcttcg ccgaccaatt tgactaatga ttttttcatt cgttcatttt ggtaagtata
    22501 gataatttct gccccttgtt tcaacattgc ttcagcacaa ccccaagcaa tgcttttttt
    22561 gttagcgacg cccataatga cgattttttt tccttctaga aatgacataa tgactctcct
    22621 ttgtttctga taaaaatttg atagtcgtaa aaaaatagag aataatagct aaaataactt
    22681 cttgatttct taattattag tcaaaatgtt ttgattgtca aactattttt tcttaaatta
    22741 aaaaaagatt gctaaatatt taggaatatg atactttgat agtcaaagta atgaaaatgg
    22801 caattgcctg aaagaaggag tttaattatg tcttacgtat tatctgctac tgaagtaatg
    22861 gaattgattc ctaatcgtta tcctatttgt tatattgatt atgtagatac tttagaacca
    22921 ggaaaaaaga tcgttgcgac aaaaaatgta acaatcaatg aagatttttt ccaaggtcac
    22981 tttcctgaca atccagtgat gccgggtgcg ttgattctag aaacactggc acaagcaggt
    23041 tcgatactta tcctaaaatc agaagagttt ctaggaaaaa gagcttatat cggtgggatc
    23101 aataaggcca aattccgtca aaaagttgtt ccaggagatg tgctgaaatg tcattttgaa
    23161 atcaccaaaa tgaagggatc agtcggtaca gcgttatctg cagcatatgt agatgataaa
    23221 aaagtctgtg agtgcgagtt cacgttcatc gtcaaccaaa atgcttgact ataaaacctg
    23281 atatagcaaa acaaaaggaa gcttacttgc tttttatgta aaaagaagac gcctgaatcc
    23341 tagcatacgt tgtcatctat gcaggattca gacgttattt ttttattttt gttttgcttc
    23401 ttgcagtata ttgattttat cgatttttac ttcttctttt ggtttgtctg aactatctgt
    23461 ttcgatattt gcaatagcat ccacgacatc cattccttca atcacttggc cgaagacagt
    23521 ataatggcca tctagtgaag gataaccgcc gtttttataa gcgtcgatga ttgcttgagg
    23581 atagtcgtct tttaaaagac catcatgcat atcatctgta ttttgaacga taaagaactg
    23641 gctgccgtta ctgttaggat cttgtgagcg agccatagat aacgcacctc ttatattata
    23701 aaggtggttg ttgatctctg tttcaaatcc ttctccccaa atgctttccc ctccagtgcc
    23761 atcgccttta ggatcgccac cttggatcat gaaatctttg atgacgcgat ggaaagtcag
    23821 tccgttgtag taaccgtctt ttgcatgtgt cataaagttt tcaaccgctt ttggtgcgtg
    23881 ttcgggaaat aatttgattt tgattgtgcc tttattcgtc accatttcga ccagatcttc
    23941 attttcggcg acatctttcg tcaattgtgg taattccaat gcgttcaaat cgacgctact
    24001 ggcagtcgtg ctttccgcag tagatgtact ttttgaactt tctgtagacg aactagaaga
    24061 agtcgtgttg tttgcagaac aaccagcagc aaacagcaat gctgctgcta aactaatact
    24121 taaaataact ttatttttct tcataactgt tcctttccaa tttactattt tgttatcttt
    24181 aatgataaca aaatagaggg aaaataccta cttaaattat tggaatttat catttatttt
    24241 aaaagaaaaa gaaaaggtgt agaccattta ctttgtgttt gctataatgt aagtggatac
    24301 aaaaacgaaa agatgcagga ggaaatggta atatgggaaa attaggaatc tcgatttatc
    24361 cagaacggtc aacatttgaa aaggacaaag cgtacttgga tttagctcat aagtatgggt
    24421 ataaacgggt ttttaccagt ttgctgcaga tcaatgatga taaagaaaaa gttctttcag
    24481 aatttaaaga agtagttgat tacgcgaata gcctaggcat ggaagtaatg gttgatatca
    24541 atccagcgtt atttgaacag ttggagattt cttacgatga cttatctttt ttccataaga
    24601 tgggtgccta cggggtccgc ttagatatcg gattcacagg agcagaagaa gcaaaaatga
    24661 cgcgcaatcc ttttggtatc aaaatcgaga tcaatatgag ttcgggcacg aattacgttg
    24721 acaacatcat gagttattcg ccaaacacgg ataatttatt aggttctcat aatttttatc
    24781 ctcatcgata ctctggttta ggatacgaac actttgtttt ttgttcagaa aaattcagaa
    24841 aatataattt gaatacgatg gcattcgtca attcgcaatc tgctgagttt ggcccttggc
    24901 ctacgcaaga tggcttgtgc acgctggaag accaccgaga tcttgaaatt gcgacacaag
    24961 tgaagcattt aatccttaca ggattgattg atgatatttc tattggaaat gcgtacgcaa
    25021 gtgaagaaga attgaaagaa atggcagaag catttaacgc ggattatcca acattgaaag
    25081 tagatacgga agaaggtatt acagaaaacg aacgaatctg cttgtttgat aaccttcata
    25141 gttatcgagg ggatcgctca gaatatatcc ttcgttcaac gatgactcgt gtatactaca
    25201 aagataaaga tttcccgcct cataatacgc gagatatgca tcatggggat gtactgattg
    25261 acaatgaagg gtacggtcaa tacaaaggag aaacacaaat cgccttgaag gatatgaaaa
    25321 acgacggacg tgtcaatgtt gtaggaagaa tctccgatga cgaattattc ctccttgact
    25381 ttttgaaacc atggtcaagc tttaaattaa ttgaaaataa aaaatagata attagttatt
    25441 gcagaaataa tccgctagaa atagccagtt atttctgctt ttttattttg tttttttata
    25501 ggttatgatt tgtcaaatta agctagaata aaatgcttct tgtacatagc tcaaaaatat
    25561 ttcgattggt gttctgtaat tcaatgattt tcttggaata tgattacgtt gattgctgac
    25621 actgg
//
LOCUS       DARBEC010000035        25194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.035, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000035 DARBEC010000000
VERSION     DARBEC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.035"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..746
                     /locus_tag="SCL80_001342"
     CDS             99..746
                     /locus_tag="SCL80_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEG1107221.1"
                     /translation="MREKTDTLPKATAKRLPLYLRYLKMLDDSGISRIKSNEFSEITQ
                     IPSATIRRDFSQLGELGRSGYGYDVPFLIDVFNNILNTKEEKRIALVGYGNLGKALKH
                     NNFRRNENLNIVCVFDNDPALINRVIDGEMIYPIDRFAEIAKAKNVTVAISTVPSKYS
                     QSAIDEIVKGNVTAILNFAPDRVTVPAYVNVQYIDLTTELQTLIYFDENYSEVFS"
     misc_binding    875..1091
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1139..3784
                     /locus_tag="SCL80_001343"
     CDS             1139..3784
                     /locus_tag="SCL80_001343"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1107222.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            4094..5407
                     /locus_tag="SCL80_001344"
     CDS             4094..5407
                     /locus_tag="SCL80_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1107223.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            5397..6050
                     /locus_tag="SCL80_001345"
     CDS             5397..6050
                     /locus_tag="SCL80_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1107224.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            6105..6656
                     /gene="radC"
                     /locus_tag="SCL80_001346"
                     /pseudo
     CDS             6105..6656
                     /gene="radC"
                     /locus_tag="SCL80_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            6787..7872
                     /locus_tag="SCL80_001347"
     CDS             6787..7872
                     /locus_tag="SCL80_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1107225.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7869..9761
                     /locus_tag="SCL80_001348"
     CDS             7869..9761
                     /locus_tag="SCL80_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1107226.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            9768..10145
                     /locus_tag="SCL80_001349"
     CDS             9768..10145
                     /locus_tag="SCL80_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1107227.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            10299..11081
                     /locus_tag="SCL80_001350"
     CDS             10299..11081
                     /locus_tag="SCL80_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1107228.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SCL80_001351"
     CDS             complement(11207..11938)
                     /gene="erm(A)"
                     /locus_tag="SCL80_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1107229.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11996..12055)
                     /locus_tag="SCL80_001352"
     CDS             complement(11996..12055)
                     /locus_tag="SCL80_001352"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
                     /protein_id="HEG1107230.1"
                     /translation="MGTFSIFVINKVRYQPNQN"
     gene            12447..13109
                     /locus_tag="SCL80_001353"
     CDS             12447..13109
                     /locus_tag="SCL80_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1107231.1"
                     /translation="MNTIESQFDKVAEDYDFVNELLNDYSFFVSNMSPKKGRALDIGC
                     GSGLLVEKLASYYDEVVGIDISNQMLDLAKSKRQLTNTVYLNMNAEQLNFNEKFDFIV
                     SRTTFHHLDDIASVIQQMKELLNEEGRIVILDNVSEVETPPTYVYKLGAIQEFLPHCF
                     KFGIKNAIRIYNHNTSKSWLEHLASDKYLSEQNYYDLYEKLLPGCQFHKMGWAMGVVW
                     TK"
     gene            13344..13487
                     /locus_tag="SCL80_001354"
                     /pseudo
     CDS             13344..13487
                     /locus_tag="SCL80_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein"
     gene            13637..13837
                     /locus_tag="SCL80_001355"
     CDS             13637..13837
                     /locus_tag="SCL80_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1107232.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
     gene            complement(14091..15770)
                     /locus_tag="SCL80_001356"
     CDS             complement(14091..15770)
                     /locus_tag="SCL80_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1107233.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
     gene            complement(15772..15984)
                     /locus_tag="SCL80_001357"
     CDS             complement(15772..15984)
                     /locus_tag="SCL80_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEG1107234.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            16377..18107
                     /gene="efrA"
                     /locus_tag="SCL80_001358"
     CDS             16377..18107
                     /gene="efrA"
                     /locus_tag="SCL80_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEG1107235.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            18104..19864
                     /gene="efrB"
                     /locus_tag="SCL80_001359"
     CDS             18104..19864
                     /gene="efrB"
                     /locus_tag="SCL80_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEG1107236.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            19959..20156
                     /locus_tag="SCL80_001360"
     CDS             19959..20156
                     /locus_tag="SCL80_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107237.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            20309..20794
                     /locus_tag="SCL80_001361"
     CDS             20309..20794
                     /locus_tag="SCL80_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEG1107238.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SCL80_001362"
     CDS             complement(20898..21491)
                     /gene="clpP"
                     /locus_tag="SCL80_001362"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEG1107239.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            21671..22198
                     /locus_tag="SCL80_001363"
     CDS             21671..22198
                     /locus_tag="SCL80_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107240.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            22289..23026
                     /locus_tag="SCL80_001364"
     CDS             22289..23026
                     /locus_tag="SCL80_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1107241.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            23030..23947
                     /gene="miaA"
                     /locus_tag="SCL80_001365"
     CDS             23030..23947
                     /gene="miaA"
                     /locus_tag="SCL80_001365"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEG1107242.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            23949..25178
                     /gene="hflX"
                     /locus_tag="SCL80_001366"
     CDS             23949..25178
                     /gene="hflX"
                     /locus_tag="SCL80_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEG1107243.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
BASE COUNT         8390 a         4103 c         5297 g         7404 t
ORIGIN      
        1 atttgtctca cacccttttc cttcctactt gtaaccttgc tcccgtgtta gaaacatgtt
       61 acactaaata tgtgaaatta ttaacaagga gcgtcgttat gcgagaaaaa acagatacac
      121 tgccaaaagc aactgcgaaa agattacctt tatatttacg ctacttgaag atgctggatg
      181 actcaggtat aagtagaatc aaatcaaatg agttcagcga aatcacccaa attccttctg
      241 caacgatccg cagagacttc tcgcaattag gcgagctagg cagaagcgga tatggctatg
      301 atgtcccttt tcttatcgat gtattcaata atatccttaa tactaaagaa gaaaaacgaa
      361 tcgcattagt aggatatgga aatcttggca aagcattgaa acacaacaat tttagaagaa
      421 atgaaaatct aaatatcgtg tgcgtctttg ataatgatcc agcactgatc aatcgagtaa
      481 ttgatggaga aatgatctat ccgattgatc gtttcgcgga gatagccaaa gcaaaaaatg
      541 tgacagtagc aatctctacg gtaccaagta aatattcaca atctgctatt gatgaaattg
      601 tcaaaggaaa tgtaaccgct atacttaatt ttgcgcctga cagagtaact gtcccagctt
      661 atgtcaatgt tcaatacatt gatttgacaa ccgagttgca gacgcttatt tactttgatg
      721 agaattactc agaagttttt tcttaggaaa atttgagtag gcttattgat cttgcgagaa
      781 ctcgtcgtaa ttctttcaaa aattttttag tactaaatga agtggttttt tcacgaccac
      841 ttgttgacta aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa
      901 ctggcacatt cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt
      961 gaaggtagct tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta
     1021 actcacttga gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat
     1081 acgtcctaga atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat
     1141 gacggaagaa aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta
     1201 tcagcattgg ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta
     1261 ttcaatcgta atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga
     1321 tacgaccttg caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg
     1381 gctgcctggt atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag
     1441 agagcaaggg atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga
     1501 atggaaagaa gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt
     1561 agattacagt agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt
     1621 ttttgttacc ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga
     1681 tcctcaagca cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc
     1741 cttttatcat atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac
     1801 ccgtccagaa acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta
     1861 tcaagcgctc atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat
     1921 tgcagatgaa tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca
     1981 tgatccaaac gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga
     2041 tgatgatggt acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc
     2101 tcgtaaagca atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat
     2161 gatccacagt gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac
     2221 tcaatggttt gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga
     2281 tcaagcagtg gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa
     2341 tgtccatgat tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta
     2401 tcacaaagaa acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg
     2461 ggtacaagat cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac
     2521 aatgggctgg ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt
     2581 agtaaccggt tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga
     2641 atttactggc gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca
     2701 agggcggaag atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa
     2761 gtatggagca gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt
     2821 ccgcttcagc tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc
     2881 ttcacgtttc gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg
     2941 agaaaaatca gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt
     3001 aactgagcta tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat
     3061 ctgggatgat ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga
     3121 tgctgtttca aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg
     3181 tttattgcat ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca
     3241 gggaaattct ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac
     3301 agcttctaaa gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc
     3361 agaagtgaat acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa
     3421 aatcgatcaa tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga
     3481 gttgacgata tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg
     3541 tgcagaaatc ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt
     3601 agaaaaagaa ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa
     3661 tgaacgtttt gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa
     3721 agattattta gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca
     3781 ataagaatag aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga
     3841 acatcgttac aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga
     3901 cataaatttt gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg
     3961 tggatcatgt atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt
     4021 ttctctttgt catactttta tttttcttat gtgttctatt tgttataata aaagagaaat
     4081 gggggaattg ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt
     4141 tggttcacgt cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga
     4201 gagaaaagta aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta
     4261 tttgagaagc ctattagaag aggctgggct gacagtaggt acatttactt caccttatat
     4321 cgaaacgttc aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat
     4381 ccagcttgta aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc
     4441 tggtattact gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa
     4501 agtggacgta gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc
     4561 tgtaccaatg ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga
     4621 tacaattgaa gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt
     4681 aacaggaaat attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa
     4741 tgcaccgctt tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat
     4801 gtggggagaa gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc
     4861 gcttttaggg caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta
     4921 ctgtcaattg gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc
     4981 tcgatggcca ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc
     5041 ccataatacg catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata
     5101 tcaaatcaac attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg
     5161 attgcagcaa gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat
     5221 cgaattaagt gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca
     5281 attcggatta gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg
     5341 ttcactttat ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga
     5401 agtataatgc agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt
     5461 acgaagccac acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt
     5521 taaaattctt aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg
     5581 aatttggcaa aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac
     5641 gtttttcgca aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag
     5701 aagaacatca gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt
     5761 tattgaagaa gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc
     5821 aacgagcaaa accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga
     5881 agaaagaaac attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg
     5941 agatcccggt agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa
     6001 cagctggggt tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct
     6061 cttttcgtat cttattttag ataagataga aaggagatgt ttttttggga aaacgattga
     6121 tcaaagaagt acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag
     6181 ctttatcgga tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg
     6241 tgatgtcgat tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg
     6301 ctactttgca tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca
     6361 aagccttgat cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg
     6421 ttcgtaccag ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag
     6481 aacatttggt ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt
     6541 tcatcggttc gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac
     6601 gttattgtgc agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca
     6661 tagtataata aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta
     6721 ctttaaataa aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg
     6781 gaacatgtga aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta
     6841 ctagataaag aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat
     6901 actggtaagt ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag
     6961 ttcatggaac agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc
     7021 gtaggttggt tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc
     7081 ataagagaag aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat
     7141 ttaagtagat taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat
     7201 ttcaaaggat ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta
     7261 agggttaaaa agaaacagat aagaacattg agatcagagg aagttaagca aattattgat
     7321 gcttgtcata cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga
     7381 atcggtgaag tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat
     7441 ttaacaccta gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca
     7501 atacatgtga gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt
     7561 gaggaattgg agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca
     7621 ctaaagtacc aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa
     7681 tttacatcac atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat
     7741 gttgcgttcg ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat
     7801 gttcatcttt cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag
     7861 cataagaaat gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa
     7921 aaatagctgt aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa
     7981 gaaaatgtcc acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt
     8041 gggttcgttt tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt
     8101 tttaccattt aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag
     8161 taattaataa aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg
     8221 aagttcctat tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta
     8281 gaattaccac tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta
     8341 attcctacta tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg
     8401 atggagagga gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata
     8461 aggttaaccc aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt
     8521 ttaaacaact tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg
     8581 taagtgatat tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac
     8641 aagttcagag agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa
     8701 acatgatggg aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat
     8761 ttagtaccct tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg
     8821 aggactatcc gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc
     8881 aattgaacag tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc
     8941 aagaatgtgg aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg
     9001 acaaagatgg agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag
     9061 ttccaatatc taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg
     9121 aatttccaga tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa
     9181 catttagagg tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg
     9241 agatttatag attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca
     9301 acgggatgcc ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa
     9361 gcagatacgc tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa
     9421 aactggttac caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg
     9481 tagagcttca atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta
     9541 gattgccagt agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact
     9601 tctgtaccag taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt
     9661 tattagccat agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta
     9721 aagagcgttt agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag
     9781 ttagaaatac aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa
     9841 aagtagacaa agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa
     9901 tagcaaagga agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa
     9961 gaatcgaatc ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa
    10021 gttctcgttc ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa
    10081 aagaaaataa aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag
    10141 aataattatt aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct
    10201 ttattgaaca gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat
    10261 aaactttata tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa
    10321 aaataccaaa tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa
    10381 tagttggagt atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg
    10441 tagatgttct agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag
    10501 aaagactaat gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag
    10561 ttacggttat aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta
    10621 tatacggtga gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg
    10681 atcctgattt ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc
    10741 ctgattcttc aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg
    10801 attctttgcc agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc
    10861 tagctcgaat gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag
    10921 aatgggctat acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag
    10981 gctatcgggg agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg
    11041 ttaagtatat gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa
    11101 caggtgctta cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga
    11161 ataaagcgtc ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt
    11221 tgtaactatt gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat
    11281 ttgcatgctt caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt
    11341 ttacccactt ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt
    11401 gatgtcgttc aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg
    11461 gtggtacttt tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc
    11521 gttgcagatt ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag
    11581 cctgactttc aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac
    11641 catatatctt atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca
    11701 cttttatatt ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa
    11761 tttctatagc agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc
    11821 ctgatccgat ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt
    11881 ttacatgctt tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta
    11941 taaccttctc cttactggtt ataatgaact gacattaagc gttcattata accaattaat
    12001 tttgatttgg ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt
    12061 tatctccttt ccattttttt cattatgaat gagataaagt aatttctact actgcgatac
    12121 tggtgcacat aattgaaacc tccttatgta actgaatatg attgtatcat cttttcattt
    12181 tagaataaac ctttatatgt aattttgcac ttgacacttc agatggacgt tttcttcaaa
    12241 ataatatgtt ttgaaacttg ataagagaag ccactgtctt tactaaaaag taaaggctgc
    12301 gataaaggac agtgacttta ataaagttgc actgttgtat tgtctttgga taaaattgtc
    12361 taaagggaag ttttatcccc aacgaaaaaa catctaaagt ggtaagtaat gaaagaatgt
    12421 cgataaagta ggaatggggg atctttttga atacaataga atcacagttt gataaggttg
    12481 cagaagatta cgatttcgtg aatgagcttt tgaatgatta ttcattcttt gtgtctaata
    12541 tgtctccaaa gaaaggcaga gcattagata tcggatgtgg ctcgggtttg ttagtggaga
    12601 aattagcaag ttattatgat gaagtggtag ggattgatat ttctaatcaa atgctcgatc
    12661 ttgccaaatc taaacgtcaa ctaacaaata cggtctatct gaatatgaat gcagaacaac
    12721 ttaattttaa tgagaagttt gattttattg taagccgaac aacctttcat catttggatg
    12781 atatagccag cgtaatacag caaatgaagg aactgttgaa tgaggaagga agaatagtta
    12841 ttcttgataa tgtatctgaa gtcgagacac cgcctaccta tgtttataaa ttaggggcaa
    12901 tccaagagtt tttaccgcat tgtttcaaat ttggaataaa aaatgcgatt agaatttata
    12961 atcacaacac atctaaatca tggcttgagc atttagcttc agataagtac ctatctgaac
    13021 aaaattatta cgatttatat gaaaagttgt tgcctggatg ccaattccat aaaatgggtt
    13081 gggcaatggg ggttgtctgg acaaaataac ttaaagctgt ggctttgaaa agttgaattt
    13141 gtgattgttt tcagagctat cgggtgcttt agtataaata acaatgaata aaaaatcagc
    13201 ctgtatctta tgttactatt aagtaattta agatacaggt tttactattt ttgaaataag
    13261 taccttccac ttcaaataca tgtattcaaa gtgaaagtat tgatttaaat gattcttttg
    13321 taatttactc ttgataatat gaaattccat cgcagcagga tatgaatttt accaaaagga
    13381 tacaaaagtg tggagaaatg atggggatca ctgtactgga ccacttgatc atcggacgca
    13441 aacgatattt tagtttgaga gaagaaggaa tgatggaaga gaagtaagtt gctttcttgc
    13501 aaaaaaaatg aagcaagtgt acaattatat tgtaattttg tttgggaata gaagttgaaa
    13561 catctgttgc acctctatcg tacacgagca atgttacaat ttaaattagt gcttttggca
    13621 cgaggaggat attattatgg aaaacggaac agtaaaatgg ttcaacgctg aaaaaggatt
    13681 tggatttatc tcacgtgaag atggaagcga cgtattcgtt cacttctcag ctatccaagg
    13741 tgacggattc aaaacattag aagaaggtca agcagtgact ttcgacgttg aagaatcaga
    13801 ccgcggccca caagctgcaa acgttgtaaa agcttaattt tatctgtaaa tcaaattgat
    13861 ggtagacatc aatttttacg agagaacaaa gcgagtaaca aaaatacctg ccacaagaga
    13921 caatttctct tgtggcaggt atttttttgt atgaaaaagt aaataaattt aagaggctat
    13981 gaaagagcca ccttgtatca aggaataaga acaaacaaaa atggcttctc atgtttttca
    14041 tttgttttct cttatattca ggacaaggtg ctgatttcat aacctcttat ttattcttca
    14101 tctggtgtca ttaccattgg caaaatcatt ggatgtcttt ctgtacgttc gaataagaaa
    14161 ggctgaagcg cgccagtcat cgcttctttc aatttatatt ctgaacagtc aggtccattc
    14221 aaacattctc ttaaagcgtg gaataagatt cgctgagctt cattgatcat ttctcctgat
    14281 tctcgcatgt agacaaatcc acgagagaga atatctggac cagccaagat ttcttttttc
    14341 ttgatatcca ctgtcgcgac agctaataca agaccttctt ctgaaagcag gtgacggtca
    14401 cgtaaaacga cattaccaat atcaccgatc ccgctaccat cgatatacac atctccagcc
    14461 acaaaatgcc cagcaggacg cgcagaatcg ctagttaacg ctaaaacatc gccattttcc
    14521 atgatgaagc agttttctgc aggaacacct acatcttgtg ctagagaagc atgtactttc
    14581 aacatgcgga attctccgtg gacaggaata aaatattttg gcttcatcaa acgaagcatt
    14641 aatttttgtt cttcttgtcc accgtgaccg gaagtatgga tgttattgat ttttccgtga
    14701 atcacttctg caccagcttc agacagcaaa ttgatcaaat gattgacact tgttgtatta
    14761 ccaggaatag gtgaacttga aaagatcact gtatcgtctg gctggatttg gatttgacgg
    14821 tgagtaccat tcgcgatgcg gctcaacgcg gccatcgctt ctccttgaga acctgtacat
    14881 aggatcatcg tttcattggc aggcaagcga ttgatttcgt gtgcatctac aaatgtgccg
    14941 tctggaactt tgatatatcc taaacgttga ccgttgacga tggcattttc catgcttcgt
    15001 ccgaaaacag cgattttacg accagttgcc acagcggcat ctgctgcttg ctgcaaacgg
    15061 aagatgttag aagcaaagct ggcaaaaatg atacgtcctt cgatttttga aaagatcttc
    15121 aaaatagaat gcccaatcgt tttttctgat ttagtaaatg ttggaatctc agcatttgta
    15181 ctatcagata aaaggcacaa gactccttct tccccgatac gagccatttt atgcaagttt
    15241 gctggttcgc caacaggagt gaaatcgaat ttgaagtctc cagtcgctac gatatttcca
    15301 gaaggtgttt tcaccacgat ccctaatgca tctgggatac tatgggtcgt tctgaaaaag
    15361 ctgacagaag tctttctaaa acggatgatc gtatcttcat tgatctcatg gagctctgca
    15421 tctcgaagca agccatgctc atctaatttg tttgtgatca acgctaatgc gagaggacca
    15481 gcatagatag gaatatttgc ttgtttcagc aagtagggta ttccgccgat gtggtcttcg
    15541 tgcccgtgag taatcaccaa ggcttttact ttgtgtaagt tttgtatgat gtagctgtag
    15601 tcgggaatga catagtcaat tccaagtaaa tcgtcttccg ggaatttgat cccggcatca
    15661 atcaaaatga tttcatcttg gaattgtacc ccatacgtat ttttcccgat ttcacctaat
    15721 ccaccaatag caaagacgcc tgtctcgtta tttttgatgg aaagtttcat attaaaactc
    15781 cactaatgag aaatcagcat tttctttttc gtactctaga tgacttcctt ctagaagttg
    15841 aacgaattca atattgtatg gggtattttc ttccacacgt tggcgagcgt ctacatcgct
    15901 ttttgcctca atgtaaagag attttgtttc ttcccgttta gggttgcgta cttttgtttc
    15961 ttgataataa actttataga tcattgtttt gtctccttta tctgcgtaaa gcagtgattt
    16021 agctagttga tttttttaga caacacaaaa cgaggggaca agccacctgg tttttcatat
    16081 gctttgacta gaatactgga ttcgagtcaa agagacaagc ctcgtttttg cttatctgtt
    16141 cggatatttt ttcaaccgta ccttctgtat aaaaaatata caacaattaa ttgttattct
    16201 agcataaaac agcaaataag tatagaaatc aactagattg aaagaaaaaa tcaatgttta
    16261 tcatcccgct ataatcataa tgactttttt atgagagaag tgttctatta ataggctcgt
    16321 tgtgctatca tttttagtaa tgaaaatgta acaaaagatt aaaaggagat gagaaagtga
    16381 aactaatgtg gcattatacc atgcgttata agaaattctt gtttttcaat ttcatatgcg
    16441 tatttggttt tattttgatt gaattaggac ttcctacgat tttagcacgt atgatcgatg
    16501 taggaatccg caatgatgat tttgattatg tgaagcaaca agggatattt atgatcgtta
    16561 ttactgtaat cggtattatt ttgaatatct tgttaggtta ttttggttcg aggattacga
    16621 ctaatatcgt agcagatatt cgagatgatt tgttcgaaaa gatccaaagt tattcgcatc
    16681 aggaatacga aaatctaggc gtatcttcat tgatcactcg tacaacaaac gatgcttatc
    16741 agatcatgtt gttcctgcaa aatattttac gtatcggttt tatggcacca atgatgttca
    16801 ttgtcagttt gtacatggtc atgcgtacaa gtgtgacact cagtttgtat gtagtaggtg
    16861 cattaccttt actattattg gcagtcgtag cgattgctaa ggtgtctgaa ccgttatcta
    16921 aaaagcaaca aaaaaactta gataaaatca acagtatctt gcgggaaaat ctttcaggat
    16981 tgcgtgtgat tcgtgcattc gtcaatgaaa agtttgaaga aaaacgtttt gctgtagtca
    17041 atgagaatta tgcttctagt tcaaaaagct tattccgttt gatggctgtt gcacaaccgg
    17101 gtttttattt cttatttaat atcgtaatgg tattgatcat ctggagcggg gcagtgcaaa
    17161 tcgatgcagg cgagcttcgt gtaggagatt tgattgcgtt tatcgagtat atattccatg
    17221 ccttgttctc attcatgtta tttgctagtg tgttcatgat gtatccaaga gcagctgttt
    17281 cagctggacg tatccaagaa gccctagatg ccaaaccttc gattgtagaa gatccaaatg
    17341 gtatatctga tacaaaaact aaagggtata tcgaatttca aaatgtaaca tttgcttatc
    17401 caggccattc gcaagagcca gtgatacgaa atgtcagttt tacagcctct ccaggagaga
    17461 ccgttgcttt tattggaagt accggtagcg gaaaatctac gttgatccaa ttgatccctc
    17521 gcttttatga tgtgagtgaa ggaaagatct tgatcgatgg agtagacgtg agagattatc
    17581 ggctaagcaa gttgcgagat aagattgggt ttattccgca aaaagcgctg ctatttactg
    17641 gaacgattgc agataatttg cgatacggga aagaagatgc tacgcaagaa gaaatggaac
    17701 gtgcagctga gattgctcaa gcttcagaat tcatttctcg aaaaccagat ggatttaatg
    17761 aacatttatc agaaggtggt tccaattttt caggtggaca aaaacaacga ttggcaatag
    17821 ccagagccat catacgccgt ccggaaatct atatttttga tgacagcttt tctgcattag
    17881 attatcaaac ggatgcgaag ttacgtgctc gtctgaaaaa agaaacgaca gaatctactg
    17941 tattgatcgt agcacaacgt gtgggaacga tcatgcatgc agataagatc atcgtattaa
    18001 atgaaggaga agtcgtcggt atggggacgc atcgggagtt gttggaaaaa tgcccgattt
    18061 attatgatat tgccgcttct caattatcaa aggaggaatt agcatgaaaa aaaccttttc
    18121 ttccttaaaa cgtttagctc gatatatcag accgtatcgt gagacttttc ttttagtgat
    18181 cctatttaca gttctgacag tggctttcaa cgtggctttg ccatatgtta caggacttcc
    18241 tactacagaa atcagccgta atattgccaa tggcgaatca atcaattttt cttatatatt
    18301 caaatgtctc atttggatca cagttgttgg aatagggtat tgtatttcgc aattactttc
    18361 tggtgttttg atgacaaatg tcgttcaaag tgccatgcat gatttgagaa gagacataga
    18421 agaaaaaatc aatcgtcttc cggtttcata tttcgataaa aaccagcaag gaaatatttt
    18481 gtctcgtgtg acgaatgatg tagatgcagt cagcggtgct ttacaacaag cttttattgg
    18541 tatcgtaaat gcgatattgg gaattacgtt ggctgcagca atgatgtttt atatccagcc
    18601 gatgatggcg ctgatctcta tgatcatgat tccaacatcg atctggatct caaaacgagt
    18661 gatcaatgct tcgcagaaat attttcaaag catgcagaat tctcttggtg agctaaacgg
    18721 ctacgtccaa gaaaatatga caggttttag tgtattgaaa gtatatggac gcgaaaaaga
    18781 aacattggaa ggattcaaaa aagttaatca cagcttgaaa tactttgggt ttcgtgcagc
    18841 attcatttct ggcttgatga tgcctctagt tcaattaact gcctatgcta cttatattgg
    18901 tatggctgtt ctaggaagct tttatgttat tactggggcg atcgttgtcg gtcaactaca
    18961 agcattcatc caatatattt ggcaaatcag tcagccaatg gggaacgtga cacaattgtc
    19021 ttcagttttg caaagtgcat cagcggcaac gaaacgtgta ttcgagattt tagatgagcc
    19081 agaagaaaaa gaaaacaaag tagatgcttc actgccagaa acgattttag gagatgtgac
    19141 attcgatcat gtcgattttg catatgatcc gaagaaacca ttaatccaag atttgaattt
    19201 tgaagtgaaa gctggtcaaa ccgtagctgt ggtcggtccg accggtgccg gcaagacgac
    19261 attgatcaat ctattgatgc gtttttatga cgtagacaaa ggagcaataa ggattgatgg
    19321 aatcgacacc aaatcaatga gtcgcagtga tgtgcgttcg ctatttggta tggtcttgca
    19381 agatgcatgg ctttatcaag gaacgattgc agataatatc cgcttcggaa agcttgatgc
    19441 gacagattat gaagtagttg atgctgcgaa aacagcaaac gtagatcatt ttatccgaac
    19501 gatgccagat ggatatgata tggccatcaa ttctgaggga gacaatgtgt ctttaggaca
    19561 aaaacagttg ctgacgattg cccgagcagt ggtctccgat cctaaaatct tgattttaga
    19621 tgaagcaaca agttcagtag acacccgttt agaagcgttg attcaaaaag caatggatcg
    19681 cgtgatggag ggacggacaa gtttcgtcat cgctcaccgt ttatcgacaa tccgtgatgc
    19741 agatctgatt ctagtgatgg atcaaggtca gatcattgaa aaaggaacgc atgagagttt
    19801 attggcacaa ggtggatttt atgaaaaact gtataacagc caatttgctg aggaagcaga
    19861 ataagtcata taagaataaa acagtttaaa acttattttt tgttctctat tcttgataat
    19921 ggtaaagtaa agttatcaag aaaagtgagg acacggagat ggaacagttt ttaaatgaaa
    19981 aaattactga agccaaaaga gaagttgatc gtttaacgca cgtctgtcag acgaagcctt
    20041 ttgattcagc tgattttttt gaaaatgagt ttcaaatcga acgcttggca gcacaaatag
    20101 aagtgtacga attaatttta aaaaaagtaa tagaagaaaa tatagatgta caataaaaac
    20161 aggtaaaacg tcagttacca gatactgtaa atagacatct ggcctgagca ggttttacct
    20221 gttttttttc ttatgaaata aatatatgct atgatacctt tattcaatag gattagtttc
    20281 tacaaaaaat gaaaggagaa aaataaatat ggaacggttt atagcatcaa atgccacagt
    20341 gattggtgat gtgacattgt cagaagatgt gacgatctgg tatcaagctg ttttacgcgg
    20401 ggacagtaat tggataaaaa taggacaaag aacgaatatc caagatggta caatcatcca
    20461 tgtcgatcat gatgcacctg tagatattgc ggagaatgtg acggtaggac atcagtgcat
    20521 gctacatggc tgtacaatcg aaaaaggggc gctgatcggg atggggacca cgatattgaa
    20581 tcatgcggtt attggtgaga atagtttgat tggtgctgga tcattagtaa cagaaggaaa
    20641 agttattcct cctaatgtct tggcttttgg tcgcccagct aaagtgatcc gtcctttgac
    20701 aaaagaagaa atacaaaaaa acaaagaaaa catccagcat tatgtcgaga taggtcggat
    20761 gcatgcgcaa ggaaaattca aggagcaaag ataggcagta gcatccataa aataaaaaag
    20821 gatggtcagt ttgagttgac catccttttt tatttggttt agataagaga gcttaaaaaa
    20881 gcttcctttg aaacagatta atttaatgaa gaactgtttt ccatgacttc atcgatcaat
    20941 ccatatgctt ttgcttgttc agctgtcata tagttgtcac gatctgtatc tttttcaatc
    21001 acttcaagcg gttgtccagt gcgttcagct aaaattttgt ttaagcgttc gcgtgtttgc
    21061 aaaatatggc gtgccgcaat ttcgatttca gttgcttgcc cttgagcgcc gccaagaggt
    21121 tgatggatca tgatctcagc atttggtaaa gcaaatcgtt taccttttgt accagctgtc
    21181 agcaagaagc ttcccattga agcagccata cccataacaa ttgtttggac atctgctttg
    21241 acaaagttca ttgtgtcgta aatagccata cctgctgtaa ctgaaccacc aggagaattg
    21301 atataaagat agatatcttt ttcagaatct tgagcgtcta agaaaagcaa ttgggcgatg
    21361 atcgaattag caagatcatc tgttacttgg ccgcttaaca tgatgatacg gtctttcaat
    21421 aaacgtgagt agatatcgta agctctttca cctcgtgaag attgttcaat gactgtagga
    21481 attaaattca taatagagtt cctccttgat ttcgtttaga aatagtgtac cataatttag
    21541 tttctaatac ctaaaatgta gtatacattt ttggtcaaaa aaggtcaaat aaaaaactta
    21601 ggcaaattag tcgcataaac ccgctttatg gtatgataag cagagaaaca aaacaaggag
    21661 gaacaacaga ttgatagttg gtattactgg ccttatcgga gtgctgacag gtgctgtagt
    21721 tgtctatatc ggcatgatac tgcaaatgcg tcacttggaa aataaagaac agcgtagacg
    21781 tgaattggaa ttgaaagtca aagaaatcga gacgctgaat caattaaata aaaagatcaa
    21841 cgagatcttg caaaaacgga caacgctgat ggaagaatat gtcagctttg atgcatttga
    21901 tgattgttat atcacgattg acgattttgc gtatctccaa tcattttcag cgcaaaatag
    21961 tttttatctg ccaaattata ttttagacga gctatttaag aatatctccc atcgaaaagt
    22021 gattttatct ccagatgaaa caatgaagat tggtggatat gcttataaag gcggacgcgt
    22081 gattttagag aattttacgg atagtctgac tgaaatgatc aatgaaaaaa aagctcagtt
    22141 gaagactatg acgcgccagc cgattggttt cttctcgaaa gatgatgttt ggagctgata
    22201 ttttgaagca ttgccgttgc acatgagagc gtggtaatgc tttctttttt tcttaatgat
    22261 acaatagaac tgaacggagt gaagaaagat gttacaaatc tatgcccatc gcggaagcag
    22321 cggcacccac ccagaaaata cgcttcctgc atttgcagaa gctgtacgag taggtgcaga
    22381 tggtatcgaa ctagatgtcc atctaagcaa agatggttat ttgatcgtta tgcacgacga
    22441 agaagtggac cggacgacta acggtaaagg gctgattcga gaaaaaacat tggaagaact
    22501 caaaaaattg aatgcagggg gcaaattcag caaagaattt ccagcagcta aagttcctga
    22561 attgagagaa gtgttaggac tgctcctcca aaaaaattac cgagggatgc taatgattga
    22621 aattaaaact gatcagtatg aatatcccgg tatcgaagaa aaattagccg aaacattaaa
    22681 aagtagagat tggccttttc aacatgcata ttgcagcttt catttggatt ctttggaaaa
    22741 aatcgcatta ctggaaccgg agatacagct ggacttattg atgagtactt ctgataaaaa
    22801 gatccaacta gcagcagaaa ccccttatat agaaggagta catccaaaaa atacttggtt
    22861 gttcgaacat ccagtccagg cagctgattt tccaaaagca attcgtgcat ggacgattaa
    22921 cgatgaagaa gaaatggcca aatgtcaaga actcaaaata gatggaataa taactgattt
    22981 tccggagaaa gctcttcggt ggaaacaatc aacgaaaagt aggtaaaata tggaaaaagt
    23041 attagtgatc gtcggtccga cagcagtcgg taaaacagcg ctaagtgtgg aactcgcaca
    23101 actatttgga ggagagatca tcagtggtga ttcattgcaa atctataaaa atctggatat
    23161 cggtacagca aaaatcaaag aagaagagat gaataatgtt ccgcatcatt tgattgatgt
    23221 gatcgaacct actcaacgtt actcagccgc tgattttcag cattcaggtc gacaattgat
    23281 taccgagatt gcgaaccgag ggcacctacc tatcatagct ggtggtacgg gtctctatat
    23341 ccaatccctt ctatatgatt atcagttagg agcgacagaa gaagcgaatc ctgagattag
    23401 aaaaaagtat gaaacgttag ctgaaacaat tggttcccaa gcattatgga atcagttgaa
    23461 agaaaaagac ccggcagctg ctgagaaaat ccactggaac aatcaacgca aagtgataag
    23521 agctctggaa gttttagaga cgacaggaca tagtatcacg gcacctaaag aagagccaaa
    23581 gcaattgtat gattacctca tgattggatt agatacgaag cgtagtattc tttatgaacg
    23641 aatcaatcaa agagtcgatt tgatgctagc agaaggttta gtagcagaag cgcgtaaggt
    23701 gtatgagctt ggagaagtcc aggccagtca aggtattgga tataaagaat ttttccctta
    23761 ttttgaaggg aaagaaacat tggaagaagc aacagaacaa gtcaaactac attcgagaag
    23821 atacgcgaaa cgtcagttga cgtggtttcg taaccgtttg gatgcacagt ggtttgatct
    23881 tataagtgag ccaaatcaaa aaagtcagat tgagcaaaca atcaaaaaat ggctggaggc
    23941 ggaataaaat ggaagaaaaa gtgatcatcg taggggtaga aaccgaagca aaccagcgtt
    24001 acttttcaga gtcaatggaa gagctagttc agctgacgaa taccgcatca ggagaagtcg
    24061 tattcacaat tacacaaaag agaccacaag tggatcggca aacgattatc ggtaaaggga
    24121 aattacaaga attagttcaa caagcggatg cgcatgaagc ggatttgatc atctttaatc
    24181 atgagctaac tccgagacag agccaactga ttaccgatgc tgttggtctt ccggtaattg
    24241 atcgcgtgca gcttatctta gatatttttg cgatgcgtgc tcgttcgaaa gaagggaagc
    24301 tgcaggttga acttgctcag ttagaatacc tgctgcctcg tttagtaggg caagggaaaa
    24361 cattatctcg tcttggcggt gggatcggaa caagagggcc aggtgaaaca aagttagaaa
    24421 cagaccgccg acatatacgg aataaaattt ttgctgttaa gaaagaattg aaagaagtag
    24481 aagctcatcg agagcgcaat cgccaaaaaa gaaaaaatag cgagattttt cagattggac
    24541 ttattggcta cacgaatgcc gggaagtcaa ctatcttgaa tctattgaca caagcggata
    24601 cttattcgaa agatcaattg tttgcaacgt tagatccatt gactaagaga tggcgttttg
    24661 ctgaaggatt tgagattact gtaacggata ccgtcgggtt catccaagac ctgcctacgc
    24721 aactgatcga tgctttccat tctacattag aagaaagtca aaatatggat cttttgctac
    24781 atgtagtcga tgctagttct ccagatcgta ttttacagga gcagactgtc ttaaaattga
    24841 tggatgaact gaatatgaaa gaaatgccga tactgactgt ttacaataag gcagaccaga
    24901 tcgacccagc gatgttcacg cctacgctat tcccaaatgt attgatttct actcagacga
    24961 aagaaggtaa gatggcgttg atcgaagcaa tcaaacgtca gctgatggaa ctaatgaccc
    25021 cttatacact ttctgtgcca agcaacgaag gacagaaatt aagcgagttg agaagacaaa
    25081 caatggtttt ggacgaagcg tacatggaag aaacaaacga atatgaagtt aaagggtttg
    25141 caaaaaccaa ttcaaaatgg ttaagaaaaa accaataaaa ttttattttt ttaa
//
LOCUS       DARBEC010000036        25090 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.036, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000036 DARBEC010000000
VERSION     DARBEC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25090)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25090)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25090
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.036"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCL80_001367"
     CDS             complement(459..1088)
                     /gene="udk"
                     /locus_tag="SCL80_001367"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEG1107244.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1172..1867)
                     /locus_tag="SCL80_001368"
     CDS             complement(1172..1867)
                     /locus_tag="SCL80_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEG1107245.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1880..2596)
                     /locus_tag="SCL80_001369"
     CDS             complement(1880..2596)
                     /locus_tag="SCL80_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEG1107246.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2926..3534
                     /locus_tag="SCL80_001370"
     CDS             2926..3534
                     /locus_tag="SCL80_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEG1107247.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEEESTGEGGEPEVIGSTEE"
     gene            3859..4182
                     /locus_tag="SCL80_001371"
     CDS             3859..4182
                     /locus_tag="SCL80_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEG1107248.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4364..5734
                     /locus_tag="SCL80_001372"
     CDS             4364..5734
                     /locus_tag="SCL80_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEG1107249.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5864..7165)
                     /locus_tag="SCL80_001373"
     CDS             complement(5864..7165)
                     /locus_tag="SCL80_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1107250.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYYYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKTE"
     gene            complement(7519..9477)
                     /locus_tag="SCL80_001374"
     CDS             complement(7519..9477)
                     /locus_tag="SCL80_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEG1107251.1"
                     /translation="MTGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9654..9920)
                     /locus_tag="SCL80_001375"
     CDS             complement(9654..9920)
                     /locus_tag="SCL80_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1107252.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
     gene            complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCL80_001376"
     CDS             complement(10430..12556)
                     /gene="pnp"
                     /locus_tag="SCL80_001376"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEG1107253.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCL80_001377"
     CDS             complement(12744..13013)
                     /gene="rpsO"
                     /locus_tag="SCL80_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEG1107254.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            13264..13827
                     /locus_tag="SCL80_001378"
     CDS             13264..13827
                     /locus_tag="SCL80_001378"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEG1107255.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(14080..14601)
                     /locus_tag="SCL80_001379"
     CDS             complement(14080..14601)
                     /locus_tag="SCL80_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEG1107256.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(14604..15446)
                     /locus_tag="SCL80_001380"
     CDS             complement(14604..15446)
                     /locus_tag="SCL80_001380"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEG1107257.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(15439..16164)
                     /locus_tag="SCL80_001381"
     CDS             complement(15439..16164)
                     /locus_tag="SCL80_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEG1107258.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            16340..16636
                     /locus_tag="SCL80_001382"
     CDS             16340..16636
                     /locus_tag="SCL80_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107259.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            17204..18796
                     /locus_tag="SCL80_001383"
     CDS             17204..18796
                     /locus_tag="SCL80_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEG1107260.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            18809..19549
                     /locus_tag="SCL80_001384"
     CDS             18809..19549
                     /locus_tag="SCL80_001384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1107261.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
     gene            19717..21066
                     /locus_tag="SCL80_001385"
     CDS             19717..21066
                     /locus_tag="SCL80_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1107262.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(21194..21784)
                     /locus_tag="SCL80_001386"
     CDS             complement(21194..21784)
                     /locus_tag="SCL80_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1107263.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVIVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            22020..23099
                     /locus_tag="SCL80_001387"
     CDS             22020..23099
                     /locus_tag="SCL80_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006908283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1107264.1"
                     /translation="MTQNHDNTVSRKGKHLTYSERCQIAILKKENYSNRQVADVLGCV
                     PQTINNEIKRGTVTQLNRQNQNGKVYDYYHDVYDADAGQAAYEKNRLNCGRRPLWADT
                     DAFIEWADDMMLLEKWSPDVVVGFARKHELFDPAIIPGTTTLYQWIDRGIMKTKNMDL
                     LEKLSRKPKSSRHGNRPNKRVLGKSIEERPEAVGTRKTFGHWEVDTVVGNKTKSDAVL
                     LTLVERQTRFEVILKLAGKDAESVNQAVRQLRERAGAYFGQLFKTVTSDNGSEFAGLH
                     DALQDVANIYFSHPYASWERGTSENQHKLIRRFIPKGSPIGNVSENQILRIQRWMNDY
                     PRKILGYATPHDTFVQAFRQERLTA"
     gene            23320..24672
                     /gene="brnQ"
                     /locus_tag="SCL80_001388"
     CDS             23320..24672
                     /gene="brnQ"
                     /locus_tag="SCL80_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEG1107265.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         7177 a         5134 c         4886 g         7893 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaac agttggctgg tgactgttca aaaagcagaa tccttgcttt
      421 tcaaacggtc gcagtccgtt caactgtttc gctgcgcact atttatggtt taagaaacta
      481 gctacttttg ttgtaatcaa atcaatagca acgtggtttt ctccgccttc tggaacaatg
      541 atatccgcat agcgcttcgt tggttcgata aattgatgat acatcggttt aacaactgtc
      601 aaatattgtt cgatcacgga gtccaatgtg cgtccccgtt cttccatatc ccgtttgata
      661 cgacgaatga tacgaatgtc atcatctgta tctacgtata ctttgatatc catcaagtca
      721 cgcagacgtt cgtcttctaa aatcaggatt ccttctagaa tgatcacttc ttttggttcc
      781 tgaatgatcg ttgcctgact tcttgtatgc gctacatagt catacacggg tttttcaatg
      841 gctttatagt tcagtaagtc gcctacatgc tgaatcagca gatcattatc aaaggcaaat
      901 gggtgatcgt aatttgtgtt caatcgttct tcaaaactca aatggctctg atccttgtaa
      961 taagaatctt gttcaagcat catgatggaa tggtccggga agttgttgaa aatcgcacgg
     1021 ctgacacttg tctttccgct tccggatcca cccgtcacac caatgataat tggcttgctt
     1081 tttgtcattg actaaaacct ctttcgtatc cgttttttca ttctaatcta ttatagcgaa
     1141 aaaacacgaa aatgctatat ttttctgaaa attaatcctt ttcaatttcc aataatgaat
     1201 aaagttcctc taatgaatcg attcgataag taggctggat ttcttcgatt tctggtaatt
     1261 tatttctatt gaaccaaatc gtatcgatcc ctgccatatt tcctcctttg atatcagaag
     1321 tcaaagaatc accaatgatc acagtttgct ttttatcaaa attaggaatg tgggaaaaga
     1381 catgatcaaa aaattctttc atcggctttt gcgctccgac agcttctgag acaaagatat
     1441 ccttgaagta atcaagcatt cctgcatctg ttaatcgccg atgttgcgtg tgcgacacac
     1501 cgttcgtcac aatataaaga tcagcatgcg gatggacctt tttcaggatt tctagacttc
     1561 ctggaacaag atcatgacct tggttcaagt agtggcggta gcgtttttcc atttgatcac
     1621 tatctactgt tttcccaaat tgctggaaaa gtaacccaaa acgactgcct attatttctt
     1681 ctcgtgatag tctgcctttt tcaaactcct gccataaatg atgattcaaa gccttatagc
     1741 tggtttcgat ttctggtgtc aatgtgattc cttgttctgc aaacaaagca cttaaagcat
     1801 tggcttcagt taattgaaaa tctaacaatg tatcatctac atcaaacaat aaagttttat
     1861 atttcatctt tttgacctcc tatttaaaat aaatacttaa ctttttcatc tctgctaatg
     1921 tcaacgatcg atactcgcct actcctagct gttcatctaa ttccaatggt cccatgctta
     1981 gccgtttcaa tgcggtcact ttctttcctt gggctaagaa catctttttg acttgatgga
     2041 attttccttc ttggatcgtc aataaaaccg tactttggtg cttgtctgct ttgagtattt
     2101 ttaaatcagc cggcttacat tgatacccat cagcgaatac gattccattt tcaaatgctg
     2161 ttacgtctgc cggtgtcacc ctttcgttca caactacttg ataagtcttt gaaacctttt
     2221 tttctggaag tagcatttca tatcccaatt gtccattcga agtgatcagc aatagtcctg
     2281 ttgtatctct atctagtctt cccactggat ataatggttg gaaacgatcg gcacttgcaa
     2341 tcagatcgat tactgtcgtc ttttctggat ctgatacagc tgtaacagct ttttcaggtt
     2401 tattgagaag ataataggac tcatttgttt ctaagtgttc acctgctact tcgatttgat
     2461 gcagtaaact atctacattt cgattttgtc gaaattcgac tgtgccatct atcatcactt
     2521 gcttcaaagc aaacagccgc ttcatttcct tgcgtgtcgt ttctaatttt tcttctatta
     2581 atttatctaa acgcatgctg tttcctttct tattactact aattaattat accttgaata
     2641 ttcttgcttg caaatacatc atcatagtta ttccttgact taattaacag ttcacgttac
     2701 ggttaaatag taaagtgaat tggatttctc atacacagcg taatgacctg gtggagaaaa
     2761 gttgaatcct gactttctaa ggggagatgc gcgcatctct ccttttgcgc ttttttaagc
     2821 gccctgcctt cgatgatgtg cacgagatta gcctttagaa ttcacttaaa taaaatctgt
     2881 ccgttaccaa agcagacagt cacattttaa ggaggaattt tggttatgtc agtatcatta
     2941 gaagtaagta aaagagaagt acgtccccgt tcattaagaa acaaacttcg tcacgaaggg
     3001 aaagttccag ctgttgttta tgggtataaa gtagaaagta cccctatctc attcgatggt
     3061 ttgacactaa gcaaattgtt gcgtgaacat ggagcgaaca cagtcatcac gatggacatc
     3121 gatggacaaa aagtcaatac attggttcac aagatccaaa cgaacacttt taccaatcat
     3181 ttcgaacatg cagaatttct atctgtaaac atgtcggaag aaacagaagt cgaaacagaa
     3241 gtcgttcttg ttggcgaatc agtgggtgct aaatctggtg gagtgttagc tcaaaacttg
     3301 tatacagtag tcgtatctgc tacaccagac aaattgcctg aacgaattga agttgacgtg
     3361 acaaacttgg aattaggtga ttctattaca gtagccgatc ttccaaaaaa cgacgattac
     3421 aaattcgtga ccgacagtga agagcaaatc gctgctgtag tcgctcaaag tgaagaagaa
     3481 gaaagcacag gtgaaggtgg cgaaccagaa gtgatcggtt caacagaaga ataatactga
     3541 aacaatcagg tggtgttaga agcggcgggt cagcagcttt taacacgcct tttttatttc
     3601 taatcgttta ttttcatatg gggcttccac ttttgtaaat ggctttacaa acgaaaaatc
     3661 ctaatttacc aagcttttcc tcgcatactt ttatcgtacc tttataaaaa tttatacttt
     3721 gaaaaaaagt attcaattat tgttgcattt ttcctgattc tttattagaa tgaaccctcg
     3781 ttgaagtaag tatttctagt ttatcctagg attaattata aaaataaaac attcgttgat
     3841 tcgaaggagg ttaaacagat gtttgaaagt tttgatagca gccgcaaccg ttacgcttca
     3901 gttggtgtcg tatcaagttt gccagatgaa ttgattgaca gtatatggtt tattattgac
     3961 ctagatctta agggagtcat tccattagaa aatatattaa cctttgattt gatcgaaaat
     4021 cgtggaaaag tgacgatgca tttttctcaa gcaggaagca atgtggaaat ggatattgat
     4081 ttgccgtttg cctattcgaa ccaatttcct gctcaagtat acgcctatga tgacggtacg
     4141 agagaaacga tccttttacc aagtgagatt agacaatatt aaggtgatag ataaacgtgg
     4201 atgtccctta ttttacagac caggccaagt tgttgattct ttgactcaat tcttggcttc
     4261 tttttatgga gtttcataaa ttatcattga tttctaattg gtattctcag gataaacgct
     4321 atatactact aactaaattt taaagatata aggagtttaa aagatgcgcg atttaacaaa
     4381 aggaagcccc gctaaactga ttttgatgtt cactgttcct ttactgatcg gaaatgtttt
     4441 tcagcagttc tacaatatga tcgatatgat catcgtgggt cagacgctag gtaaaaatgc
     4501 gctggcagcg gttggtgcaa ctggaagcct gacatttttg atcattggct ttgcccaagg
     4561 tttaacagct ggtttagcca ttattaccgc acaacgttac ggtgcaaaag attatcgggg
     4621 tctgaagaaa agttttgctg ctagtgttgt cattagtctt gtcgtcactg tgattttaac
     4681 tgtcctaagt ctcctgttta ttcgtccgat gcttcaattg atgcaaactc ctcctgaaat
     4741 acttgatcaa gcacaaacat ttatttcaat tattttatta gggatctttg catcagtcag
     4801 tttcaatttg ctttctaatg tcattcgcgc acttggtgac agccgcacac cactattctt
     4861 tctgatcatt gcggtcatca tcaacgtcgt acttgatttg atctttatta tctattttgg
     4921 catgggtgtg gaaggtgcag ctattgctac agtaatcgca caagtttctt ccagcgtact
     4981 gtgtctagtg tatatcaaga aaaaaatgcc tttgttacag ttacgcaaaa aagatttcaa
     5041 atttgaaaaa gaagagtttg ctgtacacct aaatgctgct ttgccaatgg cgtttcaatc
     5101 ttcgatcatc gctgttggtg ctatcgtgtt acaagcagca ctaaatagtc tagggacaga
     5161 tgtcgtagct gctcaagccg ctgctggtcg tatcgaccaa tttgcaaatc agccgatgat
     5221 gtcatttggt attgcaatgg caactttcac tgcacaaaac tatggtgcga aagaatatgg
     5281 ccgaatcttg caaggggtac gtcaaacttt actgatgagt gtcggattta gtctcttagc
     5341 aggtgctatc gtcattttct tcggtcattt ctttgttcaa ttgtttgtta gcccatcaga
     5401 aacacgtgta tttgaattag cacaaaccta cttcaatatc aatgggtctc tttactggat
     5461 cttagcagtc ctgttcattt tacgctacac acttcaaggg cttggacaaa gcaagatccc
     5521 tacgatcgcc ggtatgatgg aattgcttat gcgttctttt gcagctatcg ttcttacagg
     5581 aatgtgggga tacccaggag cagcagcagc ttctccttta gcttggatcg gttctgttgc
     5641 cgtgttgctt tactcttata tgcgttcgat gaaacaattg aaacacatgg gtgaagagga
     5701 acatttcaca ttagaacatg cagaagcaag atagccttga ttaaaagaaa tcgttgcggc
     5761 taaaaatgaa aacttttatt gaaaatatct tcttcgttta ccgaaaacag gttttttaga
     5821 taacaaaaag gctgtgacaa aaaatgtcac agcctttttt cttttattct gtttttcttt
     5881 ccgtggactc tactttgaaa taatcgatat gtgcatgctg gtattctaat accgtgaaac
     5941 ggaatgatgc aatctcaatg attgttcctg cttttatttc tttatatcgg ctagaagcaa
     6001 agcccgcaag tgtagtaaaa ttcgtttctt caaactctgg aatctctaca tgaaaataac
     6061 gctggaaatc atctaatgtt agtttgccgg agatgatata agagccatct gactgttccg
     6121 ctatcaatgc ttcgtccaaa tctgtctcat ctcggatttc cccgacgatt tcttctaaaa
     6181 tatcttcaat cgtcactaag ccgcttgtcc ctccgtattc atcttttaag atagcaatat
     6241 gcttaccctt ttttcgcatg atcgtcaata atttttttat agggatcgtt tcagttgtta
     6301 cgatcggctc ttcaatcaac tgttctactg tcagctggtc atctttataa gattgcttga
     6361 tcaaatctgg caaggtgaca taacctaaaa cattgtcttt tgattctaag ataaccggat
     6421 agcgagtatg tccttgttca atcgaagctt ttatcgcttc tttgacggtt tctgaagctt
     6481 cgaatacttg catggacgta cggtctactt gtatatcttt tgccaaggta tcatcaaatt
     6541 caaacacgtt ttccaaataa taatattctt ctttggtcaa ctctcctttt tcataagaat
     6601 tcaatgcgac taatgtcagc tcttcttgag acaatgtctc ttctccttca gatacgagac
     6661 gcacacctaa tagcttccct agccctgcag ctgaatgatt caatacccaa ataaaaggaa
     6721 acattacttt ataaaaataa tgaagtggtt taactacgaa caagacgacc ttctccgtat
     6781 tgacgatact atagctctta ggaagcagtt cgccaataac cacatcgata tacgtaacta
     6841 aaataaatcc cagcgccaca gagattgtac tgctaatagc cgaactaaaa ggtagtttac
     6901 cgatcactgg ttctagtaaa gccgcgacag atccttcacc gacccacccg atgatcaaac
     6961 cggttagcgt aattcctaat tgactagctg ataaatagtt atctaaatgg tgtgtaacat
     7021 gaagagctag cttggcgttc tttacatttt ccttttctag ttgttccaat cttgatcgtc
     7081 tgattttcac taaagcaaat tcacttgcta caaaaaatga agtaattgca atcatcaaaa
     7141 ataaaataat gaaattgata accatattct cccgcctttc tctttttcta ttgtaccctt
     7201 tttctacaaa aagattgaaa ataatgcgta ttcatattta ggaataaaaa aggagatgcg
     7261 gcatttttca gccacatctc cttatcactt caaaatcatt gcgttacttg ttgctaaaca
     7321 gctttttcat gacctctgat aaaaacggta ttcaaaagct tgcatctacg gcattaagcc
     7381 caaacagatg aaaaacatga gaagctgttt ttatctgctc gttcttactg cttgttgcag
     7441 tacagctttt tcatgacctc tgataaacgg tgttcaagta gctgacattc gacattagtt
     7501 caggtcaaag cgctactttc acaaccgctc tttcaaccat gttttccgag tatttccaat
     7561 gataagcgga acagtttcat gttttacttc gctgtactct agcccaaacc atgtttctgg
     7621 cgaggtagcg atttttttga ttgccagttt tgcctgcatg atttgacgtt gccattttcc
     7681 tagagttgtc acattggaaa ctacttcatc aatcaggaca aattggaaat cgccaacttc
     7741 tcgccctggt gtcagagagt atctttgtgg ctgttttggc aaacggcctt gtttcatcaa
     7801 atcatggatg acttggcgaa catatacgtt gatttcctgt tggaccctga aacctaagaa
     7861 cagattcact tggacaataa aatcagtgtc catcatatct acttgatatt ctttcgtata
     7921 tggctcatcc gtcacttcca catgaacaaa ccaatagact cgtgctcttt taggccgttt
     7981 atctaaaatc gaatagatga actgtcggcc aactttatct tcagccatat caggaaccaa
     8041 gaagaccaca tttgtctgat agattggtaa agaagtatca tcacgtaatt ctctaagctg
     8101 cgggatataa tctctcaaag acacatcctc ggttgacgat tcgcgaataa tgttcccttg
     8161 ttcccaaata aacatcacga ctaagatgat caaaccaatc agaacggcta caaaaccgcc
     8221 tcggaagaat ttgacaacac ttgagataaa gaaaacgact tctatcgcac cgaagaaaaa
     8281 ggtaactaaa tatgcaagcc aacgagaatg cccattttgg ataagataaa agtaaagcag
     8341 tgctgttgtc atcaacatcg tcacagtgat cgatagaccg taagcagctt ccatatgatg
     8401 agacgttcgg aatgtgacaa ccactagaga acaggcgatc cataaaatca gattgactgc
     8461 tgggatatac atttgcccga tactgttccc aggatacatg atctttaacc gaggcagcag
     8521 tttcaacttg attgcttctg aaactagcgt aaacgaacct gtaatcaatg cttgcgaagc
     8581 aatgactgca gcgactgttg caaaagcaac gccgatcacg atccaacttt gaggcatcat
     8641 ttggaagaat ggattcaggt tcttgatttg ctgtgcttca gggttttgat aaacattcaa
     8701 caaccacgca gcctgcccaa aataattgat aaccaaacaa attttgacgt aaggccagct
     8761 cagacgaata tttcgtttgc cgacatgtcc aagatcggaa taaagcgcct ctgcacctgt
     8821 cgtagccagg aaaatatttc caagaatgaa caagcctaat ttgttatctg cactaaaaag
     8881 cagctggata gcataataag gattcagtgc acggataact gtccagtctt gactgaagtt
     8941 cattaatccc attacgccta aaaaagtaaa ccaagcgaac atgattggtc cgaatgcttt
     9001 tccgacaata tctgtaccaa agcgttggac catgaataaa acaaaaataa taactaaagt
     9061 gataatgacg atcacgttct ggttattacc gaaccggtca aaaaactgcg gaattcctcg
     9121 caagccttca atcgcagtcg ttaccgtcac agcaggggtc aataccccat ctgctagtaa
     9181 ggcagctcct ccgatcattg caggaatgat caaatatttc ccaccttttc ggacaagtgt
     9241 gtagagtgag aaaattccac cttcaccatg gttatctgca ttcaatgcaa tcacgacata
     9301 tttgattgtt gtcagtagag tcaatgtcca aaaaatcaat gagactgatc cgataacaaa
     9361 atcaggattg attcctgaca atccgccatt atcttcaaca atcgatttca taacgtaaag
     9421 cggacttgtc ccgatgtctc cgtagacgac tcccatcgct acaagcaggc cagtcattga
     9481 tatctttttg gtattgtgcg ttgttgccac gtttttccct catttcttgt tatatggata
     9541 tcctttaggt aagcttgaat gatagatgat attcttatcc atgacacccg ctttcctaaa
     9601 ttgcttctaa ttatccaagt aacacactga agtgtcaact tatttgaata aaattattct
     9661 ttctttggac gttctctgat acgtcttgcc acaaatgaat aaagagcaag gcaaaaaatt
     9721 gcacccaata atgctggtat caaataaaaa ccgccgagtt ccggtcccag tggtcctagt
     9781 aaaaattcgc cgagccaact acctaaaaaa ccgataatga tatttccaga tacgcctccc
     9841 gggacttcct ttcctaaaat tgcgccagct atcaaaccta atataccgcc gataaataac
     9901 gaccataaaa aattcagcat ctcctttccc tcctttatcc atatttctca cttttatttt
     9961 acgctttatt cagaaggact ccaaaaatat gcgccaagaa actccagaaa aaaagaagtt
    10021 gtaacaaaag tgttcagctt atttccgtag aagctgcttc tggaacaccg tgaatcagag
    10081 gttgtgaaat cagcggtttg acctgagtat tagagaaaca aacggaaaaa tagttcctca
    10141 tattttgccg ttttttgagc taatatcgag ggacagctga ttgaacatcg tttattggag
    10201 aggttgtaaa aaagctgttc tgcatcaagt aataagaaca gccaaataaa aacagctcct
    10261 aatgttttta acttggctgg acttattacc gcagatgcaa gcttttgaac accgtttatt
    10321 cgaaaaaaaa ggactgtgac acggcttttg tcacagtccc cttttttcga ataattattg
    10381 tccccctgtc accttcacga tcacaagtac attattccta tgaattaaat tatttttctt
    10441 catttttcgt tgaaccatct tcgttcaaca ttgcttttct tgacagattt acgcgtcctt
    10501 gtttatcgat ttcagttact ttcacaagga tctcatcacc tagtttaaca acatcttcga
    10561 ctttattgac acgatcgttt gctaattggg aaatatggac aagtccatct tttcctttga
    10621 tcaagttgac gaatgcaccg aatttttcga tacggacaac tttccctaga taaacttcac
    10681 caactttgac ttctttggtc aaatcttcga tgatcttgat agcttttttg atcatttctg
    10741 catcagctga agcaatactt acttttccat cttgatcgat atcgattttc acaccagttt
    10801 catcgatgat tccgttgatc gtatcgccgc ctttaccgat aacatctttg attttagcag
    10861 gttcgatctg gatcatttcg atttttggtg catattggct taattcttca cgtggagcag
    10921 caatcgttga agtcaattct tctaagattt ccatacgtgc ttgttttgct tgagttaatg
    10981 cttcagttag gatttgttct gtgattcctt gaatcttgat atccatctgt aaagcagtga
    11041 taccatcttt tgtaccggca actttaaagt ccatatcgcc aagatgatct tccagccctt
    11101 ggatatcagt taggatcgta tagttttcac catcagaaac aagtcccatc gcaataccgg
    11161 cgactggcgc tttgattggt acacctgcat ccatcaatgc taatgtgcca gcacagatac
    11221 ttgcttggga agaagaacca tttgattcca atacttctgc tactaaacgg atcgtataag
    11281 gaaattcttc ttctgaaggg attacttgtg ctaacgcacg ttcacctaat gcgccatgtc
    11341 cgatttcccg acgccctgga gagcctgcac gtcctgtaga accaacagaa aattgaggga
    11401 aattgtagtg gtggatgaat cgtttgctga cttccacccc taaaccatca atgatttggt
    11461 gctctcctag aggtgcgagt gtacaagctg ataaagcttg tgtttgtcct cgtgtgaaca
    11521 aacctgatcc atgtacacgt ggcaataagc tgacctctga tgacaatgga cggatttcat
    11581 ccaatttacg cccgtcagga cggatcttat cgatggtgat caattcacgt acgacatctt
    11641 tttcaagatc ttctgtgatt tgtttaactt ctttcaacaa gtgatcttca tcttcatgac
    11701 ctgcaaattt ctcagcataa gcttcgcgga tatcgatctt cacctgctcg atattatctt
    11761 cgcgagcaag tttttcttca gtcattactg ctgctttcat tgtagcgtaa gaagcatcaa
    11821 agatttcttt tttcaatact tcatcaactt gcagtaaagt cacttccatt ttttctttcc
    11881 ctactgcttg aacgatttct tcttggaaag caactaactc tttgatagcg tcaaaaccaa
    11941 acagcaatgc accaagcatg tcttcttctg aaacttcttt cgcgccgctt tcaaccatat
    12001 tgatggcttg tttcgttccg gcaacagtca attcgatatc tgtttgttct gcttgttcta
    12061 ctgttggatt caagacgtac tcgccattta cacgaccaac ttctacgcct gcgattgggc
    12121 cgtcaaatgg gatatctgag attgatagtg ctaaagaaga acctagcatt gctgccatag
    12181 ctggcgagca gtctgtttct acactcatca cgatgttcgt tacttggact tcattacgga
    12241 agccttctgc aaacatcggg cgaattggac ggtcgatcaa acgggcagtc aatgttgctt
    12301 ccgtacttgg acggccttct cgtttgatga aacctccagg gattttccct gctgcataca
    12361 ttttttcttc ataattgatc gttaatggga aaaagtctgt atcttttgct tctttagagg
    12421 ccacagctgc acttaagact actgtatctc cataacggac taaaacggct ccgtttgctt
    12481 gttttgctag ctgaccgact tcgatttgta agggacggcc gccccatgtc gttttgaata
    12541 cttgtttttc tgacataact tctccttttc cttctcgtga aagcaagacg ctactaaaca
    12601 aatgaaaact gccgaaagcc gtcttttgag cagctttcat ttggttagta ggagtccatc
    12661 tagcttgatc acggaatttt acttaattga tatacgagta aaaaaagtga gattcatgtg
    12721 aatcccactc tttttgacta aaattaacga cgcaagccta aacgtttgat caattcgcgg
    12781 taacgttgaa cgtcagtttt acgtaagtaa gctaacaagt tacggcggtg accaactttt
    12841 ttcattaggc cgcggtatga atgatggtct tttttgtgca cgcgtgcatg ttcattcaag
    12901 tggttgattt cttcagttaa tacagcgatt tgtacttctg gagaaccagt atccccttca
    12961 tggcgagcgt attcgttgat gatttcattc ttgcgttctt ttgaaattgc catttctttc
    13021 acctctttcg attattcccc tgcgactgag taaaacgttg gtgattcgct taaccaagtg
    13081 gcaggtcatg tgcctagagc acacagctat actttacctg aaaccgggtt gaaaatcaag
    13141 tagttaatca ttaagaaaag gtagttttca aacaatctta ctactacttt tccatttttg
    13201 tggtaaacta taatgcgaaa atagacttga aacacagtct tttaagaaag gggtacatat
    13261 tccatgatta caatggatga tatcattcgt gaaggaaatc cgaccttacg cgaagttgca
    13321 aaagaagttt ctcttccatt aagcgaagaa gatatctctt taggaaaaga gatgcttgag
    13381 ttcttaaaaa acagccaaga tccaatcaaa gctgaagaac tacacttacg aggcggtgtt
    13441 ggcttagctg ctcctcaatt agatatttct aaaagaatca ttgctgttca cgtaccaagt
    13501 cctgacccag aggcagatgg accttctcta agtactgtta tgtataatcc taaaattttg
    13561 agccattctg ttcaagatgc ttgtttaggg gaaggtgaag gctgtttatc tgttgatcga
    13621 gaagtaccag gatatgttgt tcgccatgcc aaaatcactg tgtcttatta tgatatgaat
    13681 ggtgaaaaac acaagattcg tttgaagaat tatgaatcga tcgtcgttca acatgaaatc
    13741 gaccatatca acggtgtgat gttctatgac cacatcaacg atcaaaatcc atttgcacta
    13801 aaagaaggcg tattggtgat cgagtagaga attggcaggt tccttcgata ctatgctaca
    13861 agaaagcttt ataaaaaagg ggagtgctca aaagctgcac ttcccttttt tatgtttctc
    13921 cactaatcga aatttagata gacatctaga aatcgcaatt ccaaaaaatt ctgtgtattg
    13981 gatacgcatc ttttcttatt ttttcttatt tcttccatat gggtgtttat ctcagagatg
    14041 atagaatagt ttctaggata aaaagtagca gctacgatgt tatccttcgt agaggattcc
    14101 tgcttcagct agtttttgtt tgatgttttt cagcatatgc ttttctgggt agcggatcgt
    14161 atgcagatgg atgccgcctg ttaattcgga taacaaagta gcattacttt tttgataacg
    14221 tctcataaag gaatcgatat cttgtggcgt tttgatgtgc aaagttcctg ctaactcacc
    14281 gtaaagggga tgttccacaa ttacatcaat gatttcacca ccattttcaa cgattagtct
    14341 cagttcttct tctgtttgat cttttccatg ctggacagcg attttactga tgtgcccagt
    14401 attggtttct tctgtttcga ggatataacc tcttgctgtt gccacgatac ttttacctgc
    14461 agctcgtagt aaagcaatat cgcctacaat gatctgacgg ctgactccaa aagttttcgc
    14521 aaacttgcta gcactcactg gtttttctgt atttgccaag gtttgcatga tttgttttcg
    14581 ccgtgtctct ccttcgatca cacttattcc tccttcattt tgttttggta ttcgcgcgag
    14641 tgtttcatca aatcaacata cgtgtctgtt attgtttcct tatatccctt atttttcagc
    14701 agactaacaa ctttatccaa tacttgctgc tctctttttg catcataaac tgcttggttt
    14761 tcttcttttt tcacttgtcc tattttagtt accgtatcca ttcttttttc taataaagca
    14821 accaactctc gatcgatcat atcgatctta tgacgtaatg tttcaagaga ctcttctgtt
    14881 agttcttcct ctactgtctt gatgatacga gcaggatttc ctgcaatcac tacattgtcg
    14941 ggaaaagatt tggtcacaac cgaacctgcg ccgactacta cgttgtcccc tagagtgaca
    15001 cccggcgtga cgataactcc tccgcctaac caaacattat taccgatttt aatgggtttt
    15061 gcgtactcca gaccgctgtt acgtttcact ggatgtagtg gatgcgtcgc cgtgtaaagc
    15121 tggacattcg gagcgaacat gcagttatca ccgatttcaa tcgtgctgac atctaaaaat
    15181 gtacaattga aatttgcata aaaattctcc ccaacaaata tattatatcc gtaatcaaaa
    15241 ttgataactg gctccatata gatttttttc cctgtacggc caaaagtctc tttcaacaat
    15301 tgggatcgca gttccgctga ttctgcttgg ttgatgattt ggctttgact tcttgcaaac
    15361 atacggtcgg caaccagctc aggatcattg gcaaaataaa gctcgcctgc aatcattttt
    15421 tctttttcgg ttttgaattc agtcataatc tgctcctaat tcttgtattt ttttgaaaac
    15481 aaccgctccg tcttcttgaa taaacgtata aggctccgct aaataaacag gcgtttgatt
    15541 gattactaag attttggctg acggctggcg aaactgtacc agatcacaaa acggatgaac
    15601 ttgaaaactc gttccaacaa tgacgatcag ctctgcttgg ctgacggcat aagccgcctg
    15661 ctccatgact ccatcttgaa acccttcacc atataacacg atgtctgggc gtatttgtcc
    15721 tccgcatgct tgatgtttgt cgctgtgaag atattctttc cagtctaccg tttgttgaca
    15781 ttttcgacaa tgacattgat acagactccc atgaaaattg acgagttgcg ggcttttagc
    15841 ctttgcgtgc aaaccgtcga tgttttggga aacaacccaa acattttttt ctttcgctag
    15901 atgtgctatt tccaaatgaa tgatattggg ttttgcttca ggatgataaa tctttttgac
    15961 aaattgatag aatttatccg gttcattcac cattgcttga tgactcagca agtattccgg
    16021 ctgctccatc ccgtggtaga ttcctgacaa agatcgatag tctggcactc cagaaggtgt
    16081 ggatacccct gcaccggtca aaaatgtaat ttgctgataa gctaatatct cttttgctgc
    16141 ttcgtctact gttaattgtt tcatgttttg tcacctcact ttccattata gcccatactc
    16201 gaacaaattt attgttgaaa agtcagaaaa atgggaataa aaaagtgtta tgggcatttg
    16261 agcaatttat cacaatgttc actgacaaaa gaatgaaaac gtgttacaat actgctgtat
    16321 aaaagtgagg tgaatcgata tgttaccatt ctttatttat tggggcgtta ttctggcttg
    16381 tatcgcctgg ttagcgttaa gcgtttattt ctctgttttt tatcttgtac gcaaagaaaa
    16441 tggaaatctt tgggcttttg ctttattcaa cgtgattgcc gcaattgttt tagccattac
    16501 attagccgta tatcgaacat ggggctgggg aatcacccaa tactcaagtt tgatttatct
    16561 aatattagcc atttatggcg tcgttgttct tttgcaagcg atcctaggac gtgagccaaa
    16621 gaaagcggca gcttaaatga tcgagatgga actggacaca ggaaattttc tgttctagtt
    16681 ctttttttat tgtattacat acatttttgt gtataattat actatcgttt gcaatggata
    16741 gatgattcac attttgattg ttttttcggt aaaagtaaag atttctatat ttatcagtat
    16801 tttttcaaaa accagcagtt atagattgac aaagcctctc gagtatcgta aacttttttt
    16861 gtataaataa ctgaatacga gatgcctcaa tcaatgaggt agaggtcgcg agaatcatta
    16921 accctgtgga gtgaagcaaa catggagaag caggaatagg gaggatcgcc gaaatgtgca
    16981 cttttttgct tggagtgtat gttgggacgc aagttaagag cttgcggact gtcttagcag
    17041 tgatgctaag ttgcgctatg ttttgtgaga ggtttatcac cttatggaat tttagcggcc
    17101 tttttatatc tataaatcgg tcgcttttta ttttttcaga taaagcttta ccgcatttca
    17161 tctagaccgc tcgtgtatca tataatcaga aagggagaaa aaaatgaaaa aaaatatggt
    17221 ttcagttgcc gtactatttg cttttgtctt ctcactattc agtgtgggat ctctggcaaa
    17281 tgcagatgaa aaaacaccga gcgatgagtt tcgcgtaggt atggaggctg ggtatgcacc
    17341 cttcaactgg tctcaacaaa cagatgagaa taatgccttg ccaattcaag gccaaaatag
    17401 ttacgccggc ggttatgatg ttcaaatcgc taaaaaagta gcagatggtt tagggaaaaa
    17461 attagtcatc gtccaaacga aatgggatgg tctggctcct gcccttcaat caggcaagat
    17521 cgacgcgatc atcgccggaa tgagccctac tgctgaacga cgaaaagaaa ttgattttac
    17581 cgatccttat tacgaatcac aattagtagt agtagttcaa aaagatagca aatatgcaaa
    17641 agcaaaaagt ttagcagatc tttctggagc aaagatcact gctcagttaa atacattcca
    17701 ctatactgtg atcgatcaga taccagatgt gcagaaacaa caagcaatgg ataatttctc
    17761 agcaatgaga acagctttag cttctggaat gatcgatggt tatgtcagcg aacgtccgga
    17821 aggtgtaacg gcaactagtg tgaacaaaga cttggaaatg cttgaattca gcggtgcaga
    17881 tggcttccag acaaacccgg aagatgttca gatttctgtt ggtatgcgta aaggcgaccc
    17941 ggatatccaa aaagtaaacc agattctttc tggcatctct aacgatgaac gaaccaaaat
    18001 tatggaccaa gcagtcaaag atcagcctgc tgcaactgat agtgaagaaa aacctggcat
    18061 catttctgat ttcactacga tttggaaaca atacggcaat atgttcttac gaggtgctgg
    18121 acttaccttg ttcatcgcat tgatcgggac agttgtcggt actacacttg gattactgat
    18181 tggtgtcttc cgttcggtac ctgaatcagg aaatgcactg actcgcttct tccaaaaaat
    18241 cggaaatgca cttctttcta tctatgtcga agtattccgc ggaacaccga tgatggtaca
    18301 agcaatggtc atcttctacg gtctggcact cgcttttggc atttcattga accgaacgat
    18361 tgccgctttg tttattgtct ctctcaatac tggagcttac atgacagaga tcgttcgtgg
    18421 ggggattttt gcagtagatg aagggcagtt cgaagctgcg caagccattg gtatgaccca
    18481 tggccaaacg atgagaaaag ttgttattcc acaagtttta cggaatatct tgccagcaac
    18541 aggaaatgag tttgttatca atatcaaaga tacagcagtt ttaagtgtaa tcggcgtagc
    18601 tgatttgttc ttccaaggaa actctgcttc cggtgcgaat ttccaattct tccaaacatt
    18661 tacgatcgtc gggatcatct acttagtcat gacctttacg atcaccagaa tcctgcgggc
    18721 ggtagaaaag aaaatcgatg gtccttcagc ttataccaag cttgaagaaa tcgacaattc
    18781 aaatttgcag gaataaggag gaagaatcat gaatgaaaaa attattgaaa tcaaacatct
    18841 aagaaaaagc ttcggtgaaa atgaagtatt gaaagatatc agcgtaacag tcaataaagg
    18901 tgaagttgtg acgatcatcg gttcttccgg ttctggtaaa tctactttct tgcgctgtat
    18961 caatttactg gaaaaaccaa caggcggcga gatcatttat aatggagaaa atgtattagc
    19021 tcctaaatat aatttaccta aatatcgaac gaatctaggt atggttttcc aatccttcaa
    19081 cctgttcaac aatatgaatg tcttggaaaa ctgtatgtct ggtcaaacaa ctgtcctgaa
    19141 acgagataaa gaaactgcca aaaaagtagc aatcgaaaat ctggaaaaag ttggtatgga
    19201 tcgttatatc gaagccaaac cagcacaatt atctggtgga cagaaacagc gtgtcgcaat
    19261 tgctcgggca ttatcaatga atccagatgt attgctattc gacgaaccta cttcggcgtt
    19321 agaccctgaa atggttggcg aagtgcttaa tacgatcaaa gacttagcac atactggctt
    19381 gacaatgctt atcgtgacgc atgagatgga atttgcaaaa gatgtatctg accgtgtcat
    19441 ttttatggat aaaggtgtga ttgcagaaga aggtacacca gaagatatct ttgtccatcc
    19501 gaaagaagaa cgtacaaaag aattcctttc acgtatttta aatgcttagg atcaatagct
    19561 gattgggaca aagagctgcg gtgtttaccg cagttctttt ttatgaatta ctcataaaag
    19621 gtaaatttct gaaaattttg gaatcttagt tgtgtttttc agaaaactct cttaatatgg
    19681 aagtaattaa acttttttag ttaggatgat ttaaaaatga atgaagagca agaactgcag
    19741 cgtggactga aaaatagaca tgtccaactg atttctattg gcggagcgat tggaacgggg
    19801 ttgtttttag gttccggaaa gtctatccag ctagctggcc cttcgatatt gctcgcctat
    19861 cttattactg gatgcatctg ttttttcatt atgcgtgcgt taggcgaact tctgttatca
    19921 aacaccaata accattcttt tctggatttt gttgcagaat atcttggcaa aaaagcggct
    19981 ttcatcactg gctggacgta ttggttctgc tgggtatcga ttgcaatggc cgatttaaca
    20041 gccatcggaa tgtatgtacg ctactgggcg cctggtgttc ctcagtggct tcctgaactg
    20101 attgcattag gtcttcttct ttgcttgaat atggtggctg tctcattgtt tggtgagtta
    20161 gagttttggt ttgcattgat caaagttgta gcgatcattg cctttatcct cgtaggagct
    20221 tatatgatcc tcacccacta tacgacagat attggcacag ccagtatcac gaatctatgg
    20281 tctcatggcg gattctttcc aactggtgca aaaggattta tcttagcttt ccaaatggta
    20341 acctttgctt ttgctgggat agaattagtt ggattagtcg ctggagaaac agaaaatcct
    20401 gaaaaagtgt tgccggaagc tatcaataat attcctatca ggatcatctt attttactta
    20461 ggatctcttt ttgtcattat ggcaatctat ccatggaaca gcttgaatcc agataacagt
    20521 ccatttgtag aagtattttc ggaaattggg atcacgattg ctgcttcctt gatcaattta
    20581 gtcgtattaa gtgcagcagc ttctgcctgc aacagtgcaa tatatagtac aggacgtatg
    20641 cttcgttctt tagcgcaaga aggcagtgcc cctaaaaaat tcaaaaagtt aacaacacat
    20701 catgtgccag ggaatgcttt gacattttct actatcgtca ttttcatttc tgttatcttg
    20761 aattacgtga tgcctagtga agtattcaca ctagtaagca gtattgccac tacttgtttc
    20821 ttgtttatct ggagtatgct tgtctacacg cacatgaaat atcgtaaaag tatcctcggc
    20881 aaaaaagctc attcatttaa aatgccgttg tacccattta gtaattatct ggtatttctt
    20941 tacatggcct ttgtgtgtgt cgtcttgttc ctaggaaaag atacgagaat cgctttatta
    21001 ctaacacctg tgtggttctt gcttctatta ctaatctatc atatgaaata tgaacgtaag
    21061 aagtaagaaa gtagttgtga cagaagtggt cagcttcgag aaataagaag gaatttacga
    21121 aaattgcttc ttaaattttt gtgaaatttc ggcttatttc ttggagataa aagcttctgt
    21181 cccagcctct tttctatttc cccggaaaca accgctgata ataggcatct gcttttttat
    21241 aggaaaaaat ccaaatacca gatacaaaaa aacgtccgaa taacccgatc aacggtacaa
    21301 aaaagacacc ggcaatcaag cataagttaa tcatcatgga gataagactt cgacggtcct
    21361 ttgacaaaac tctcacgaca tgggaatctg gtttatttgc tttcatgatt tgcctcatca
    21421 agataccaaa agaaaaactc cacatcgtgt atactaagac ataagccatc tcaggccaaa
    21481 aagcttcagg gttacgcccg acccaactag taacagaagg acataaagtc agccaaaaga
    21541 gcaaaaagat attcgaccag accactcggt aattaattcg ttctgccatc tgaaatatac
    21601 gatgatggtt cacccagatc accgcaacaa atataaagct gatggtataa gctaataatg
    21661 tatttcggat gacccataaa gaatgaaagc tagatgtatt agggatcttg atctctaata
    21721 caataactct caaaacaatg gctaccactc catcagtaaa tgcttctaat ctcgactttg
    21781 tcacgatgat tcctcctcag atgattcagc gcttttattt tatcacttac gttattaaat
    21841 aaccatcgtt ttatataggg aattttctga aaattgttgc atatttttcg aaattttgtt
    21901 acttttaaaa aaaggcggat tttataatta acttagccat ttggaaataa aaaaacgcca
    21961 cgtgaattcc atgttatatt gaagttacca caactctcaa tagacaagga tgaattcaca
    22021 tgacgcaaaa ccatgataac acagtttcac gcaagggaaa acacctaacc tactcagaac
    22081 gttgccaaat cgccatcctg aaaaaggaaa actattccaa ccgtcaggtt gcggatgtcc
    22141 tgggctgcgt gccgcagacc ataaacaacg aaatcaaacg cgggaccgtt actcagctta
    22201 accgccagaa tcagaacggg aaggtctacg actattatca tgacgtctac gacgccgatg
    22261 ccgggcaggc ggcctacgag aagaaccgct tgaactgcgg cagacggccc ttatgggccg
    22321 acacggacgc cttcattgaa tgggcggacg acatgatgct gttggaaaaa tggtccccgg
    22381 acgtggtggt gggctttgcc agaaaacacg aattgttcga cccggccatt atccctggga
    22441 ccaccaccct ctatcagtgg atcgaccgtg gcatcatgaa aacgaagaat atggacctgc
    22501 tagaaaaact gtcccgcaaa ccgaaatcgt cccgtcatgg caaccgcccg aataaacggg
    22561 tgctagggaa atccattgaa gagcgcccgg aggcggttgg tacccggaaa accttcggcc
    22621 attgggaagt cgacacggtc gtaggaaaca agaccaagtc ggatgccgtg ctgctgacgt
    22681 tggtggaacg gcagacacgc ttcgaggtca ttctgaagct ggccgggaag gatgccgaat
    22741 cggtcaacca agcggtccga cagctgagag aacgggctgg tgcgtacttc ggccagctgt
    22801 tcaagacggt tacctcggac aacggctcgg agttcgcggg actgcacgat gccttgcagg
    22861 atgtggcaaa catctacttc agccacccct atgcttcctg ggagcgcggg acgagcgaaa
    22921 accaacacaa gctgattcgc cgtttcatcc ccaagggcag cccaatcggc aacgtgagcg
    22981 aaaaccaaat cttgcggatt cagcgttgga tgaacgatta cccgcgaaag attttgggct
    23041 atgcaacgcc gcatgacacg ttcgtccagg catttaggca ggaacggttg acggcttgac
    23101 acgtgggccc ttgacaacga gcctccctcg gcatggcttg tgtctggaaa gctggccggg
    23161 gccagccagt cgggagacta ccatgcctca gctcattgtc aagggcccac tcgattagaa
    23221 aaattagacg gttggtggta acgagcaacc gggtcaccat gggtggctaa cttaaacttg
    23281 aaatttacga cttttaaaaa aaccgtaaag gagttatgta tgacaaaaaa attatcattt
    23341 aaagactatc tttttatagg ctccatgctt ttcggtctat tttttggtgc tggaaatctt
    23401 atctttcccg ttcatttggg tcaagaagca ggcgctgcta ccttttgggc gaatctaggt
    23461 ttcttggtta ccggtatcgg tcttcccttc ttaggggtga ttgccattgg tgtatcgaaa
    23521 agctcaggtg tatatgaatt agcaaaacgg ataaatcaaa catatgcgat ggtttttacc
    23581 atcttgcttt acttagttat cggaccgttt tttgctttac ctcgccttgc aaccacatct
    23641 ttcgagattg gtttagcacc atttatcgat cctagcagac agacgctata tctagcaatt
    23701 ttcagtattg tattttttgt gctggcttgg tggttttcaa gaaaaccgac aaagatatta
    23761 gattatgttg gaaagttcct gaatcctgca tttttggttt tattaggtgc tttgcttctc
    23821 cttgcttttt tgaatccact aggttctgta aatcaagcgc ctattcagcc aaattatcaa
    23881 gagcatgctt ttttcacagg gtttactcaa ggatataaca cattagatgc gcttgctgct
    23941 ttagcatttg ggatcatcat tgtcacaaca atccaaaaca tgggtgtaac aaaacctgct
    24001 gaaatcgcta aagacacgat caaatctggt gcgatcagca ttatattgat gggaatcatt
    24061 tatactttgc tcgcttattt aggtgctatg agcttaggta gctttgctct aagtgaaaat
    24121 ggcgggatca cgctggctca gattgctgat cattatttag gtacttatgg cagtatctta
    24181 ttagcattta tcgttatatt agcttgcttg aagaccggta ttggtttgat tacagctttt
    24241 gcagaaacat tcacagattt gtttccgcaa agaaattacg cattctttgt tgcacttgct
    24301 agtatccttc cttgtttagc ggcaaacgta ggcttgacga atatcattca attttcgttg
    24361 cctgttttga tgttcatcta tccattagca atgacactga ttttactagt gttagttggt
    24421 cctttgttta agcagcgtac ggctgtttat cgaatgacta cttattttac attgattgct
    24481 tcgatttttg atgggttgaa cgcttgccca gaaactatca aacaaacacc tattgttcaa
    24541 aatcttttat atgttgcgga aagctacttg ccgtttttca aattggggat ggggtggatc
    24601 gtaccagcag ttatcggttt tgttatcgga ctgatttgga gtttcgctaa gaaagaagaa
    24661 gtagcagatt gataaaagag gttgtgaaag agacgttcag ccttgagcaa taaaaagtaa
    24721 ttttgaaaaa tggcttctca catttttgca taaatttgac ttaataccga gaaactgact
    24781 tttgaacacc gtttattgag aggtcgtgaa aaagccgctt agtttcaagt attaagaaat
    24841 attttgtgaa aatggcttct cacatttttg cataaatttg acttaatacc gagaaactgg
    24901 cttttgaaca ccgtttattg agcgtctata aactgacctg agtattagag aaacaaaacg
    24961 gcaaagtagc tccatatact ttgccgtttt gttttgaact aataccgaag agaagctgat
    25021 caaacaacat ttcttttata atatagattt tagacatata tatgatataa aaatagtttt
    25081 cgcaaaaaaa
//
LOCUS       DARBEC010000037        24575 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.037, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000037 DARBEC010000000
VERSION     DARBEC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24575)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24575)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24575
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.037"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(152..550)
                     /locus_tag="SCL80_001389"
     CDS             complement(152..550)
                     /locus_tag="SCL80_001389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEG1107266.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            complement(547..1320)
                     /locus_tag="SCL80_001390"
     CDS             complement(547..1320)
                     /locus_tag="SCL80_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1107267.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            complement(1317..2000)
                     /locus_tag="SCL80_001391"
     CDS             complement(1317..2000)
                     /locus_tag="SCL80_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEG1107268.1"
                     /translation="MLKNFKEVIAAILPMTVLIVILTFVFAPLEGEDLASFLVGAAIM
                     MIGMTLFLFGADYSMMEVGDLVGKYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQ
                     QVYDISDGEIGRVLLIGIVSVGTGVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFT
                     SSEFMPIAFDSGGVTTGPVTVPFILALAGGMTSMIKQKKNENDSFGMVGIASLGPILA
                     VMILGVIFQ"
     gene            complement(2167..3054)
                     /locus_tag="SCL80_001392"
     CDS             complement(2167..3054)
                     /locus_tag="SCL80_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107269.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(3051..3920)
                     /locus_tag="SCL80_001393"
     CDS             complement(3051..3920)
                     /locus_tag="SCL80_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107270.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(4057..5343)
                     /locus_tag="SCL80_001394"
     CDS             complement(4057..5343)
                     /locus_tag="SCL80_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1107271.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            5616..6194
                     /locus_tag="SCL80_001395"
     CDS             5616..6194
                     /locus_tag="SCL80_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1107272.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPMKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            6194..7123
                     /locus_tag="SCL80_001396"
     CDS             6194..7123
                     /locus_tag="SCL80_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107273.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            7120..8856
                     /locus_tag="SCL80_001397"
     CDS             7120..8856
                     /locus_tag="SCL80_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1107274.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCL80_001398"
     CDS             complement(8919..9755)
                     /gene="pheA"
                     /locus_tag="SCL80_001398"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEG1107275.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(9752..10261)
                     /locus_tag="SCL80_001399"
     CDS             complement(9752..10261)
                     /locus_tag="SCL80_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEG1107276.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCL80_001400"
     CDS             complement(10254..11549)
                     /gene="aroA"
                     /locus_tag="SCL80_001400"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEG1107277.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGEAHG"
     gene            complement(11562..12653)
                     /locus_tag="SCL80_001401"
     CDS             complement(11562..12653)
                     /locus_tag="SCL80_001401"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEG1107278.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCL80_001402"
     CDS             complement(12670..13836)
                     /gene="aroC"
                     /locus_tag="SCL80_001402"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEG1107279.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCL80_001403"
     CDS             complement(13838..14902)
                     /gene="aroB"
                     /locus_tag="SCL80_001403"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEG1107280.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCL80_001404"
     CDS             complement(14935..15957)
                     /gene="aroF"
                     /locus_tag="SCL80_001404"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEG1107281.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(16616..17590)
                     /locus_tag="SCL80_001405"
     CDS             complement(16616..17590)
                     /locus_tag="SCL80_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEG1107282.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(17571..19454)
                     /locus_tag="SCL80_001406"
     CDS             complement(17571..19454)
                     /locus_tag="SCL80_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEG1107283.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(19472..21697)
                     /locus_tag="SCL80_001407"
     CDS             complement(19472..21697)
                     /locus_tag="SCL80_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1107284.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            21812..22846
                     /locus_tag="SCL80_001408"
     CDS             21812..22846
                     /locus_tag="SCL80_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107285.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
     gene            complement(22913..23926)
                     /locus_tag="SCL80_001409"
     CDS             complement(22913..23926)
                     /locus_tag="SCL80_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1107286.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYWGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(24004..24192)
                     /locus_tag="SCL80_001410"
     CDS             complement(24004..24192)
                     /locus_tag="SCL80_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEG1107287.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
BASE COUNT         7061 a         5341 c         4397 g         7776 t
ORIGIN      
        1 gcatattttg aaaagaaagt ctgatgctta gagtagaatt tcataaaaat ttactatttc
       61 ataagtattc acgagaaaaa tgcaaacaaa aaagcggaaa taacgctcat ccgccttctt
      121 gtttgtgttt atttcctctt caaacgcttg tctatgattc agacttttct ctatcgttat
      181 tttcatctaa aagttcttgc attccgataa tttctcgata ttgatgaatc ttagtgatac
      241 caatgacttt gtctacatca atactaaaac aaataccatt acctggaata tcgatgttga
      301 tgccttgacc aatcgcatcc attactttat tcgtcaaact atcgggtact aatgtcaaca
      361 caatatcttt ttctggcgaa atgtccaaac ctaaaaattt ccctgaatca tgtacaccag
      421 aacctcttcc atgtaagatc gttccgcctg ctgcacctgc ttcttttgaa aagtcgataa
      481 catcttcgcc cattcctccg tcaacgatcg tcacaatcat gtgaagattc aatgctacaa
      541 gatctttcat ttgctttccc ccaatttttg caatctttct ttttcgattc tgtctttttc
      601 atcttttcgt ttataaatat atcctagtac taaagtggat aatattggtg tcaatgcaat
      661 cattgcaacc acaccaaacc cgtcaatgat cggatctctt ccttcaattt gtgcagcgat
      721 tgccacgctc attgataaaa taaatgtcga acacatcggg cccgttgcta ctccaccgtt
      781 atcaaaagcc attgctaaaa agatcttgtc cacttttcgt cctaaaataa atgctagccc
      841 gtatcctggg atcaagaaat aataaagaga gaatccgacg agcaaacgcc acatagacaa
      901 tcctactgct gcgccgatcc caattgaaat taccatcagc atgattttcg atcggattct
      961 cccctcgctc aattcttcaa cctgtttgat cattacatga atcgccggct cagcaaaccc
     1021 tactaagaac cccattaaaa agccaagggg aaccagtata taattgtaag gcaatcccgc
     1081 aatagcactg cctaaatgct gtcctaccgg tacataagcc atattgactc cgtgcaggaa
     1141 aaggatcaac ccaatagtcg ttatcaagaa acctttcata atgtcaaaga aacgcttttt
     1201 gcgcatacga aaactaatga tattcattaa aacaaatagg attacgatag gtgccactgc
     1261 caaaatgact tccatggtaa cgatttcgat tccatctatc acttgctgcc agaaactcat
     1321 tggaagatca cccccaaaat catcacggca agaatcggcc ctaacgaagc aataccgacc
     1381 attccaaaac tgtcattttc atttttcttt tgtttgatca tactagtcat ccctccggca
     1441 agggccaaga taaatggaac agttactgga ccagtagtca ctccccccga atcaaaagca
     1501 atcggcataa attcggaact ggtgaaaaat gaggcgatca aaacgcctat gtagcctata
     1561 gccatcaatt gataatagga tagcttgaat acgacccgaa gcagcgcaaa ggctagaaat
     1621 actccagtgc cgacgctgac gatcccaatc agcaacactc tgccaatctc tccgtcagat
     1681 atatcatata cttgttgtcc caaaacttgc acggaaggtt cagcaatcgt gatcacgatc
     1741 ccgatagcaa atcccaaact gaccaaaacg gtcaaacttt tctttttgat catatatttc
     1801 cctactagat ctccgacttc catcatagaa taatctgctc caaacaaaaa gagcgtcatt
     1861 ccgatcatca tgattgccgc accaaccaga aatgaagcga ggtcttctcc ttctaacggt
     1921 gcaaaaacaa acgttaaaat gacaatcagg acagtcatcg gcaatatagc agcaatcact
     1981 tctttaaaat tttttagcat gtatcctcct cctttactct ttaagtttac cttaaaatga
     2041 aagagttttc gagcacaaac ttaaaacgtt atcatttaat ttttctcaac ataaaataac
     2101 acacgtccca aaagtatctt tttacttgga aaaatgactt tgaaggaacg cgcttattgt
     2161 gtttttttat tttacagagc cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa
     2221 tacgatggca gttggtaaag tagcgatgac taatccagcc atgatcattc catagtctgg
     2281 tgtataggaa gcacccatag cagataaaac aagcggaacc gtccgtttat caggagattg
     2341 caaagctact aagggccata aataattgtt ccagctactc atgaatgtga tgattgcagc
     2401 cgcggcatag gtatttttag ccgtcggcat atacactcta aaaaaaatac ttagttcttt
     2461 tagaccgtct aatcgagcgg cttctactag atcttttgga aaagatttaa cattttgtcg
     2521 aaagaagaag atcaaaaaag cagtgctaac agatggcagg atgacaacca agaaactgtt
     2581 gattcccaaa accgttccgt tcaattgtga aaacattcga tataaaggga tcatcaatgc
     2641 cgcaaaagga accatcatcg atagcagtag aatattgaaa attaggtctt ttcgtttact
     2701 ttgataaatc tcaaatccat atcctgccat tgacgaaatc agcaatgcaa acacagtggt
     2761 caccaaagcg atcatcgctg aattccaaag ggaacgagta aaattcagct cattggaaaa
     2821 caaattttga aaattgatga tcaagttact gccaatcttt atttttcccg atgtgatatc
     2881 cacgggtgta ttcgtcattc ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga
     2941 taggatgctt aatgctgtat actttaatac tgacccaatt gtcgaacgaa gttctaatgc
     3001 ttgttttttg acgttggggc gttcgattgt ttctttttct accatatttt tcatcgactt
     3061 tctcctccta ctttcgtttg gattatcgac aatatcacaa taaaggaaac aataacaaag
     3121 gaaactgctg ccgcatatcc aaaattaggc gtgaatttat aactcaaatt atagatgtat
     3181 tgagataggg tggtcgttgc atttgctggt cccccgccag taatattttg tacttcatca
     3241 aaaagctgca atgtcccgat cgtcgaagta atggaagtaa acaaaatgat tggttttaaa
     3301 tttgggatcg tcactttgat aaactgctgc catttagaag caccgtcaat agatgctgct
     3361 tcataaatct ccgaatcaat attttgcatg cctgaaagga aaaaaatcat attgtatcca
     3421 gtccaacgcc aagtgatcga aagaatgatc agaactctcg cccaaattgg atgagtcaac
     3481 cagccaatcg gtgtgtcaat cataccaatg cccaacaaca agttgtttac aaggccagat
     3541 tgagaaaata gacttttcat gatcagagaa taactgacca tcgaagtgat acatggaaga
     3601 aagatagcgg ttcgaaacaa ccctttatat ttcaactttt tatcatttaa aaggttggaa
     3661 ataaaaagtg ctagaaacaa catgatcggt acttgtacaa gtaaatacag gaatgtatta
     3721 aaaaaagctt ttttaaaagt tgcatctgtc aacatccgct gataattacc taacccgtta
     3781 aatgtcatat tgacaggggg accactttga aaagacgtga tcaaagccaa aaccatcgga
     3841 ataaaaacca tgattgatat caaaatgact ggcaagatta aaaaagcatg cccgtttttg
     3901 aataaatctt tctgcttcat tcttttccct ccaattctat cattgattat ctacatgaaa
     3961 agacaagaaa gacaatatct gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg
     4021 atgaaaaagc gaagaaattc acttttttac caccgatcaa ttagccaatt gtgcttctac
     4081 ttgtttttgt gtatctgcta gtaccttgtc tgtctcttct ccattgatga ttcgatgaag
     4141 agcttcagct acgactgctt caattgcata ggtctcatgt ccgtaattca cttctggtat
     4201 ttcagatgtc catgttgaaa aatcgtcaaa gatcttttga ccactgtaaa attcagaagc
     4261 ttcttgataa gcttcttgtt cattcgcgct aagcatcgta gatactagtc cgatttcttt
     4321 cgcaagagtg cccatcagtt cttcattcga agcaaacgtt gcttctagga aatctttcgc
     4381 tgaatcttct ccagctactc ctttgatcac ataccagcca gcgccgccaa ggttagaagc
     4441 ttgcgctttt tgcagatttt ctggaagtgc aggtattggt gcaatcttcc attttccaga
     4501 ttggttttct gctccttgga tcgttgaaga ataccaagcg ccagttgggc tagaagctac
     4561 tgctcctgat tgtatcgcag ttactccagc attccagtca gaggtttgtt cgactagatc
     4621 ttctttcaaa agagtggcaa acaattcaag accatacttt aaagatgcat tgtcttggat
     4681 cgtgactgtc tccccatctt cagatgtata ccattctcct gcctgttgca taatcatcct
     4741 tactcgacca aggtcagaag gattgacttc agcgattttc tttcccgtct tttctttgac
     4801 atctcgcgct acttgaatat actcgtccca cttcagattc tccatatcct cttctgtata
     4861 acctgcttct gccatcagat ccgtacgata gaagtttgct gttacgcccg aatcgaaagg
     4921 aacaccgtat attttatctc ctactttgtt taccgcaaat ttatacggtg caaaatgatc
     4981 ttcctttaca atagcagaca aattagcgaa tgcatctgga taagaagtca aatagccttg
     5041 tatcctataa tcttcaatca agacgatatt gggcaatcct tctgtattcc cgctcgctaa
     5101 agcggtgttc agtttctgaa cgatatcatc ctgagacatc gtaacaactt ctatctctgt
     5161 ttcttcgttc tcataaactt tttttgcttc attcactgct ttgatattga atgtctcatc
     5221 ccacgcccag acagtgactt tttcttcttt gtctgctgta ctactagcac ttccgcatcc
     5281 tgttaagaaa cctgatgctg ccgctaaaca aacgactcca gcaattaact ttctgatgtt
     5341 catttttatc cgctcctttt ccatccatta gttgtttacg tttgctacat tcatcatacc
     5401 aaaatttgaa aacggtttct ttattattca gatacttcaa aaatttctcc taaaataata
     5461 aaaaaataca aatcaactat gacaaaagga ataatcttag catctagacc aaagatcgaa
     5521 atggtgaggt ttctttaaaa taaaaatatc cttgcatttt ctcctagaaa taagtatttt
     5581 taaattacaa gactattctt aaggcgggta aaaaaatgcc aatacgcgta attcttatcg
     5641 atgatgaaca gacgatttta gatggaatga aacagttgat caattggtct gagcacgggt
     5701 ttcaattaat agggactttt tccagtccga tgaaagcgct tgatttttct ttggtacatg
     5761 caatcgatat cgtggtcaca gatgtaatga tgcctgaatt aaatggaatc gaattatcgc
     5821 ggctattaaa acagcaacaa cctgaaacac agatccttat cctctctagc tatgatgaat
     5881 ttgatatggt taaagattct tttaaagaag gcgtcgctga ttatctttta aagcctcgat
     5941 tgagtcctga tttttttcta aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc
     6001 ccaacaaaag cccaatcacc aatcgttatg agcagatctg cgagaatctt tcacagcaga
     6061 ttgctggaca aactcgtcca ttgcttgacc ctcttctttt tgaacacacc ttgtttttct
     6121 gttatatacc gaaaaacggc cattcagttc aaaacaaggc tctaaacaaa aacagtcgat
     6181 cacccattca ttgatggata gcgacgattc gtttactatt tatcctgttt ctaccttttc
     6241 ggaagaatcc ggctatgtaa tcaatactga ttccaaagat acacttagga agtttgttca
     6301 aaagtttcgt caagaagttt ttccaggtga tttttttgtt gtttctgatt cttttgcaat
     6361 ccaagatatc aatgaaatat tccaagagat cagaaattgc accaaaggac aacgtttcta
     6421 tcataaggga aagaaccttg cttatcagca tgaacttctt ccgttattaa ccaaccacga
     6481 gcagcaagca agtagttatt taacaaaaat cgttcataaa gactacgctt tagctgtcaa
     6541 agaacttgag aatttcttca cgacgatatt ggaacagcct attgatccag cttatttgaa
     6601 acatgaggca atcaatcgtt tctatgcatt actcaacgct ttggctgacg agcatgttgg
     6661 gaatgaagaa ctttctttgc tcaagatcgc ccttccttct ttattagccg atgctgagta
     6721 tttcgaagat ttttctattc ttgtcaaaga aacacttgag aaactcaccc acttttctgc
     6781 aaatgcaaaa aaagacaatt tcgatatctt gactttgatc tacgagttcg tccaggaaaa
     6841 ttatgagaaa gaaatcaatt tacaactttt atccgaaaaa tatcattttt catatagcta
     6901 tctctcagcc atctttactg aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt
     6961 ccgtatcagt aaagccaagc tctttttgac ggaaacacgg cttactttga cggaaatctg
     7021 tgagaaagtc ggctataccg aactgggtta tttctccaga gtttttaaag aagagacagg
     7081 aatgacacct tctcaatacc gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag
     7141 catgaactct ttgccaaact tctgttcatt ttgttcatcg gtctttttct gcaagccatc
     7201 gtcatttctt tgtttattta tcatcgatca agggatgcct atatccagct gttcaatcaa
     7261 tcgaatgatg tggtactaaa aaagattcaa tctgaatttg aaagtttgaa tgatacaatc
     7321 gagaatacat tagccgcttt tgattctaat ccagctgtca aaagctattt ttccaatgat
     7381 ccagcccaac atatggaaca gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct
     7441 ctttcaaaaa tccatccaat gatcgattac gatgtattga tctttggaga aaatggaaga
     7501 acttttgtcg gaaacgacat gctcactgcc gtatctgccg attctttttt tcaatctgcg
     7561 attgctcaga gagtgaatga acgtgctgct gatacacaaa tgctttttgc acaccacgga
     7621 ttgactcttc gcgataagaa agcacccagc gtcttctttg tcaaaaaact gaaaaataca
     7681 ttgaatcacg tttacggata tgctgtttta tcgatctcat ccaagcaact agcgaatctt
     7741 tttcaatctg tagtaaatcc tgagatatcg aagatctcct tcatgaacga agaacagacg
     7801 atcatcgcat caaatgaaga aaatacaatt gggagccgat caatgatttt caaaaaacta
     7861 acagtaggtg aaacaacgat ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc
     7921 caggattgta cattactcag tcaagtcgat cttcattctt tatctaacca aatgggcgtg
     7981 atttttccga ttattttgta taatatcgtg acacttgctt ttgctggctc attggtattt
     8041 atgtatttgg atcgacacac aaaatcgatc taccgcttga ttgattcgct aaaacggcta
     8101 gaggaagatg gcttaaaagc tactgttcct gtacaaggaa gccatgaagt ccaattgcta
     8161 gggaatacga tcaatcaatt gctaagtaat gctcagcatc attatgaaca gacgatccaa
     8221 gacgaaaaga agaaacgttc gcttgaaatc caaactatgc aagcacagat acagccccat
     8281 tttatttata atacgctgac tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa
     8341 gcagttcaag gaatcgacag tttcattact ttactaagaa gtacgattgg gaaaaaagaa
     8401 gaagtgattc cagtcaagga agaaatcaaa agcgtacaaa gttacatcga tcttttgagt
     8461 atgcgatatg gcgaaagaat ctcggctaaa ataatgattc ctgatgaact gcagtcatta
     8521 tctatgccga atatgatcct tcaaccaatt atcgaaaacg cctatcttca tgcttttcac
     8581 aataaaacgc aaggattcat cacggtgtac ggcaaaacaa aaaatcagct tcttttattc
     8641 gaagtgattg ataacggcga tggcttcgat actgaaaata ccaaacaaaa aaatgatcat
     8701 ttttcaggaa tcggcatcag ccatgtagat gagcggatac gcctaatgta tggcgatgca
     8761 tatggcttaa cgatccaatc agtgattggg acaggaacga ctgtaacgat ttgtctaccg
     8821 cttcttccta aaaatcaatc agaatatcaa aaataaagaa tagccaagcg ctgatttttt
     8881 tattttaagt taaataaatc aatcagtatt cttgactttc atctacgcgt ctcttctact
     8941 gtcagcactg gataacagcc caatatttga ctttctcctc cgagcatctt gatttcttgg
     9001 agcgcattct ctactaatgt cattggacgg tccaacaaca aatcgatcac gaaaaaatat
     9061 tctcccaagc tagttttcaa tggtcgggat tcaatcttac ttaagttgat ttcccgccag
     9121 ccaaaagctg ccagcatttt atgaagcatc cctggccgat tagcaggtag tgtcaagatg
     9181 acactcattt ttactggagc tgactgctgg ctggtcatct ttcggtcacc gacgatccaa
     9241 aaacgtgtct ggttcaactc attatcttgg atattctcag ctaagatctc caatccaaca
     9301 tgctgtgctg tttctaatga agcaatcgct gctgcgtctt ctttcggatg ttctgctaca
     9361 tacattgctg cagcagttgt tgattctgtt gctactaaag gaacattagg ataatgcgtt
     9421 tctaaaaact gttgtgattg tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt
     9481 gttgctggat tgcctaaaag ctgctgcttg ataggaagaa cgatttcact tttaacttcg
     9541 acttctggat gtttggaaag aagatcgatt gtgtgatgaa cagctccttc caatgaattc
     9601 tctacaggaa cgacagccag atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg
     9661 atggaagcat acgcagtcaa ttgttctgta ggaaatagct gacaagccgc ctgataagtg
     9721 aatgaacttt ctggccctag atagctgacg ttcatatgct tttgatcctt tctataatgg
     9781 tatcgatgat cttttctggt gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt
     9841 cataccaaga aagtcgttgt gccaataatg cttttatttc ttctcttgtt tttccaagag
     9901 ctaaaggtcg aatagacgtt tcatcttgtt ggatacgatc aactagacag tccgcttctg
     9961 cctctaagta aagaacaatc ggctgtgttt ttaaaaaacg ccgattttct gcactttgga
    10021 caatccctcc accagtcgcg ataattttgt cttcatttga cagctttctt aaaatatctg
    10081 actccacttt tcgaaagctt ttttcaccgt aatagtgaaa atattcggca atcgacaatt
    10141 ggatatgttc ctcaatcttt gtatctaaat ctacatactc tttttgtaaa cattgagcta
    10201 accctttacc gattgttgtt tttccagcgc ccatgaaacc aatcagcaaa atgctagcca
    10261 tgagcttctc ctcctgacac aagttctgct aaatcgtcaa aaaatgccgg ataagagatt
    10321 gaaacagctt cagctccttc tagttcgcaa ggtccatcag ctatcaaagc agctacttgc
    10381 agcatcatac cgattcgatg atcgcctctg ctggacactt ttgcgccatg cagaggtgtc
    10441 ggcccatgga tgatcagacc gtctgctgtc gcttttatct ttgctcccat tttctgtaat
    10501 tcttctgcca ccgcatcaat tctgttggtt tcttttactt ttaattcttc tgcgtctttg
    10561 atgatcgtga ttccttctgc ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc
    10621 agacgcggaa tgatttctcc accaatcgtt gctttcttca aatgactcgt cttgacactt
    10681 aaatcagccg attggttctg ttcattgtga ttcgtctggg taatcgctgc gcccatctcc
    10741 tccaacacat ctaagatacc agttcttgtt ggattgaccc cgacattttt taacaacagt
    10801 tggctttccg gaacaagaag tcctgctgct agaaagaaag cagctgatga aatatcacct
    10861 ggaacagtta tctgttgacc agttaatttt tgcggtccgg tcactatgat cgttttatct
    10921 tccactgtga tccttccgcc aaattgacga atcatttctt ccgtatggtt acgggaacgt
    10981 tctttttcaa cgattacgga tgtgccttct gcttgtaatg ccgcaaacag caaagcagat
    11041 ttgacttgcg cactggctac aggcatattg tatcggatag gtgataaaga gcgggttcct
    11101 gtcacagtga ttggtagata ctggtcattt tcttctcctt gcaagtcagc gcccatctcg
    11161 cgtaaaggtt ccatcactcg ccccattgga cgtttagaca acgaagcatc cccgaacaga
    11221 gtagtcgtaa aaggctgtcc agccaaaata cccatcaata aacgtatggt cgtaccggag
    11281 tttcccatgt caattgattc ggttggttgg acaaatgagt ggctaccgcg accatgaatc
    11341 ttgatcacct cttcctcttc ttcgatctcg acacctaatt gttggaaggc atgtaaagta
    11401 ctcagacaat cttctgcgcg caaaaaattt tgtatagtcg tcgttccttc agcaattgct
    11461 ccaaacatga tgctgcgatg ggaaattgat ttatctgctg gtatcctaac agtccctctt
    11521 aatccatgta tttgttgtaa taattgcatt tatccagcct cctatttgat ccagcaatga
    11581 taactggtat tagacataat gattcttttt gcttgtaaca aatcctgttg gtttttaaaa
    11641 ctgatttgca gtattcctaa aatatcttcc ctggtctctt ggatcttcaa attaatcaaa
    11701 gaaactcccg cttttccgat cagtccagtt acttctgcaa taacgccagg ttcatctggc
    11761 acatcgataa ataaatcaaa aaaggcagga atcgcgcctt gcttatggac aggcaactgg
    11821 tctcgacttt cttttgcccg attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg
    11881 atccattctg ctatttgtgt catttcttgt tgccaatctg ccaatcgttc caataatatc
    11941 tgcttattgc tcaacaaaat atctgtccac atgacaggat ctgaggaagc gatcctagtg
    12001 atatctcgaa agcctcctgc tgccagttgt ttggctcgcg gatactcttg attgaattgt
    12061 tctgcctgat tgactaagcc agctgccaga atatgtggaa aatgacttag cattcctgtt
    12121 atacggtcat gttctcttgc tgtaagagtt acatattttg ctttggttcc gcaaaatagt
    12181 tcttgcagtt tttcaacatc ttttttcgtc ttttccaaag gtgtaaagat gtaataggca
    12241 ttttcaaata gtttagcatc tgctgcctgt atacctgatt tatgagaacc agccattgga
    12301 tgtccaccga caaaccgaag atttttctgc tcagcggatg tcacgacttg ctgcttcgta
    12361 ctgctgacat ccgtaatgat gacgtgatca gacaagttga ttctttcaag gacatcaaga
    12421 taagctaagg ttgtacggac gggaacggct aataagataa cgtctgcttg ttctgcacct
    12481 gtttcaaaat cacttgcaac gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt
    12541 tttgactgac tatcccaacc aactactatc agttctggat tggtctgacg aatacaaagt
    12601 gccatagaag caccaatcag tccaagcccc acgataaaaa catgttgtgc catcttattt
    12661 gccccctttt tagtaatgtt tcaagtagtc acggtactcc ttcactgcag ctttcaactc
    12721 aacaagcgaa tcacttggga attttgacaa tagctctgtc gctaccacag tggctacaac
    12781 attttccgca acgacgctag ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc
    12841 tttgtaaggt tcttttgtat cgatatcaac actttgaagt ggcttgtaca atgtaggaat
    12901 gggtttcatg accccgcgaa tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc
    12961 gcctagatta ttggtacgtc ggaaatagcc tctttcttct tcccatagga tctcatccat
    13021 cacttctgag cctggtaatg aaccagaagt gaatccgacc ccaaattcta cgcctttaaa
    13081 ggcattgata ctaaccacag cttgtgcgat tttggcatct agcttcgtgt cccattgtac
    13141 gtagcttcct aatccagctg gaacgcctcc aacgagcact tctacaattc ctccgatcgt
    13201 atctccattt ttctttgtct gatcgatcaa atcttttatt ctttgctcaa ctgctggatc
    13261 aacaactcgt acttctgacg cttcagaaat ttctttgatt tcttgaatcg agatatttcc
    13321 aggtatttct gcttggattc caccaagaca aatcacgtgt cctgctacga tgatgtcaag
    13381 ttcttccaga attttcttgg caattgcgcc gattgctaca cgcatggtcg tctctcttgc
    13441 agaggaccgt tcaagcacat tccgaagatc tcgatgatgg tatttcatac cgcctaccaa
    13501 atccgcgtgt cctggacgcg gacgtgcaac tcgccgcagt gattggtctt tttcaggcac
    13561 ttctgctaca gacatgacag ttgtccaatt tttccaatct ttattttcta ccactaaagt
    13621 gacaggtgaa ccaattgttt tgccgtgacg gatgccagac gtgatccgaa cacgatcttt
    13681 ttcgatcttc atccttcctc ctctgccgta accagcttgt ctgcgagcga gttcgtgatt
    13741 gatttcttct tctgacagag gtaatcctgc tggtagtcct tcaataatgg ctgttaattc
    13801 tggtccatgg gattctcccg cagtcatata acgcatctta cagtcctcct aagtaatctt
    13861 tgaatgtctc tgtgggaaca ggaacgattt ttgcttgccc aatcttttct agcaagatga
    13921 tattcaattg gcttcctctt gctttcttat catgggttat cgcgtggaat aattcatctg
    13981 gatgacttag atcgtacgta atcggcagat ggaatttttg aagtatcctt tctaaagcag
    14041 ctgtcgatcc agttggagac aagccgattt tttctgcatg agaagtgatg gctaccattc
    14101 caatagctac tccttctcca tgactgacta ccccatatcc tgctgtattt tcaattgcat
    14161 ggccaattgt atgaccaaaa tttaaaataa gtcgatttcc ttggtcaaag acatcttctt
    14221 ccactacttt tcgtttgatt tccaacgtag cagtaatgat tggaacagca tgttccagaa
    14281 gatctttttc gtctttcagc tcgttcagct gtttccaaag cgttgcatct gctatagctg
    14341 ctgatttgat gatttcagcg attccttctc gtaaacgacg taccggcaaa gtttttagaa
    14401 cctcaggatc aatcaataca ccagctggct ggctaaatgt tccgacaagg tttttcgctt
    14461 tgtttgtatt gactgctgtc ttcccgccaa tactagaatc tacttgtgca agtagcgtag
    14521 tcggaatttg aacgaaatgg atcccacgca tgaacgtcga ggccacgaat gctgctagat
    14581 cacccaccat tcctccacca agtgcaagta tcccatcgct gcgggtaaaa tcattatctg
    14641 ctaaaaaatc gtataactca gctgcttgtt ctaaagattt actggtctca cccgccggta
    14701 cgattgaaag gactggttca aaattagctt gtctcaattg gaacatgatt ttttctgcat
    14761 atagtggagc aacgtttgta tcagtgatca cagctacttt ttgaggtctc caaatcgttt
    14821 gaagccatga tccagcctgt tctagtgcgc ctttttcaat atgaatatcg tatttttggt
    14881 tatcaacatg tacagtaagc attcgtttca cttctttctt ttaaaaagct ggaattaatc
    14941 gagtgtctga atcttttcca ttgcttcttt catgatcgca acttctttca tcatgcgtga
    15001 gtaagtcttt tcattcaacg attggggacc gtctgaccaa gcatttgcag gatcaggatg
    15061 gatctctacg atcatgccat ctgctccgct tgccacaccc gcacgagcca ttggtggtac
    15121 aagttcccaa attcctgtac catgacttgg atcaacaatc acagggaaat gagttaattt
    15181 tttcatcaat gggacagcac ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg
    15241 aatcccacgt tcgataaaaa tgacaggaga tttgtcttcg actgcaatgt attctgcagc
    15301 attaagccat tcattgatcg taccggaaat tccacgtttc aatccaacag gtttacctgt
    15361 tttaccgaca gcagatagca gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat
    15421 atcgctgtac tcagccacca tatcgatatg cccttcatcc atcacttcag taatcacttt
    15481 cattccaaat tcatcagccg cttgacggat gtatttcaat ccttcttctt ctaaaccttg
    15541 gaaagcatat ggagaagtac gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc
    15601 ggctttggcc attcgcgcac attctctgat ttggtctaat ccttcgatag aacaaggacc
    15661 agccatcgtc acgaaactgc cgtcaccaat ttttactccg tctacatcca cgactgtatt
    15721 gccaggatgg aattctcgag aagtcaattt gtatgtgttt aaaatacgca cagcattttc
    15781 tactccttca tagcgtgtaa atgctccttc ttgtaaactt cgcgtatcac cttttagtcc
    15841 aatcactact cgttctgttc cttgatcgat tgagactttg aatccttctt tttcgatatg
    15901 atcgacgact ttttttattt gttcttccgt tgcttctttt ttcataatta ctatcattgt
    15961 ttttcccgct ttcttttgtt tacttatcta tcttggctga ttttctctac tttttcaagg
    16021 tggttttaca ttaattccta ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc
    16081 tttcctactt atgacccaga taatcaacaa aaaaagatgt ccctgtagga aattgcctct
    16141 acagtgaaca tcttcaacga gccttaaaaa gcgctgcaaa aatataaaat agccactata
    16201 gatgaatttc tctacgtggc tgtaagtcgt gtttaaaaat ctttagccat catagataca
    16261 aacctggttt gtttgtaccc acgaagtcat gaatacaaac ctatctaaaa tagctaaagt
    16321 aatacgtatt cagttttgta agttttctag atttcataaa aaatcgctcc aaactgatgt
    16381 aagaattgta cctagtatag acataaaaaa atcgactgtc aatcattttt atgaaacttt
    16441 ttttaatttt ctgatagtta actttgtttt tcagaaaaat aacaattggt tttattgaag
    16501 aaaatagtca tgaacaaaag tatctgagaa cttttttact ctcttgttca tgacatatag
    16561 aataaagcag ctttgtcttg taaactccgt ttttcaaata ataatctaaa gattgctact
    16621 gaataataaa gccgatttca tggtcctctt cgctgctgat tcggtaaggt gactcaacat
    16681 ccgctcccca gctattgata cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg
    16741 ttcgtctttc tggcggtagt tcttctatat gtgttgcctg ttccagttct tctgctttga
    16801 atggtaaagc ggaaaaagca aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt
    16861 cttgatcttt atccgtatta tccaacgttt gatttcttgt tagttctacc catttcgttt
    16921 ccatatgcat gccgcattct tggggaacaa ggtatgggga aacggggagt cctgccactt
    16981 tataaatacc ttcgacacca ccagccatcc gatccggata tgtttcccct gataggcctt
    17041 catactcaaa aaattttgct ttcgttggca taatgaacct taaacccaat gcgggtaatt
    17101 ccggtaatcc tttttgtcca taataatgca tattgacaag cattttaccc tctccagcga
    17161 tttggtagga aattgtcact tgggtttttg gtactgtgtt gatttccagt gtaaaaatga
    17221 tttcaaccgt ttcagcgtat tcattacttg aatattgatt atttactgga gcaatcggca
    17281 aatcgatctt tttattgtcc accagtactt ctatttcttt gacttttgga tacaatccgg
    17341 ctcctaacca cataccggat ttaaatccaa atccgctacc tctgtcatta tcggtcgttg
    17401 ctcgccaaaa aactggaagt gtttcccggt atagccattc ttttccgttt ttattgagcg
    17461 attccagtcc gcctcttgta taagaaaaaa tataatgcgt attccccgat ttgacaccta
    17521 atgtacagtc gccgaagacg attgcaactt tttccgtatc catatgactt ttatttactg
    17581 tatttttcat aataatatct tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa
    17641 acgatcccat tccctgagaa ttcatagtca cttggtttat cgtcaaagtc accgccatag
    17701 cgcatcactt ctctgcctgt gattggatca gtcacttgga tcgcctgatc tttaaaatcc
    17761 catatatagc ctccttggta catttcataa cgatcaagca acgtcatgta agaatccatt
    17821 ccacctaacg aattccccat gtcatgcata tattcacata agatgaacgg tttttccgct
    17881 ttattttcaa ggtagtcaat gatctcttct ggtggcgcat acatgcagct ttttacatct
    17941 gaaatcgaat cttcgaatgc tggatttgga aaattccctt cataatggac aagtcgatcg
    18001 gggtctactt ttttgaagta atcgttcatt tgtcgaatgt tttcttccgc ataagattca
    18061 tttccaagag accaaaaaag aatacttgga tggtttttta atgtttcgaa attcgttttt
    18121 gctcgatcaa gaacagccgc ctgccactga ggtactgcac ctggtacgtt gtaagaaggc
    18181 tcaaatgcgc ccattttttg ccatgagcca tgggattcga ggttcgtttc cgccatcaca
    18241 tggattcctg cttggtcgca taaataatac caagtgatct ggttggggta atgagatgtc
    18301 cgtacgccgt tgatctttgc attttttatg atagccaaat ctttttccat atcttctata
    18361 gtgatggcgc gtcctttttt cggatgccac tcatgtcgat ttaccccgtt gataatcatc
    18421 cgttttccat tgaagtagat gattttatct ttgatttcga ttttacgaaa accgaaggaa
    18481 tagtcaatat actccgttac tcttttttct tcgtctatcg tctccaattc aagcttatat
    18541 aaattgggct gaaaatgccc ccacagtgcc acattttcaa atccatcgat gcgaatcgtc
    18601 atttgctctt ttaacggata ttgctgttga tagatttcct tcccatcagg atctagtatc
    18661 gctacattca gctgactgtt tttggctact tgctcaaaag acaaatctac ttcaagtttg
    18721 ccattttgac atttgtcatc taagcgaggt ttgatccaaa gatcttctaa atggttttgt
    18781 cctcgagcaa ctaagaagac atctctgaag atcccaaaaa atcggaaaaa gtcctgatct
    18841 tccaagaatg cagctgtact tcttttgtaa acctccacag ctagaatatt ttcttcttcc
    18901 aaataaggtg ttaaatcaaa ttcagacgga gtgaacgaat cttcggcata cccgataaaa
    18961 tgaccattca accaaataaa cattgcttgt tctacaccag caaaatgaat cgagatttgt
    19021 tttcccttta gaccttcttc taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct
    19081 ttagcagctg aaaagatccc ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg
    19141 cgaaatacat gtccctccca cggatacatc gtattgatgt aatggatctt gtcataattt
    19201 gctaattcaa tatgactagg aacagtgatt tcatcaaaag aacaggcatc aaaatctttt
    19261 ttataaaatg tttttggtcg taccgaagga tttggcgaat aacaaaaacg ccatgatcca
    19321 tttaaagact gtttaaggga ggatgtggtt tctacttctt cccttgttgc ataacaaaca
    19381 tgatcgctat gtgcatttga ttggttcact ctaaaaactt gtggatcatc taaccatttt
    19441 atatttgctt tcatactttt ttccttctct ctcataactt tttgaaatga agcagttctg
    19501 tttggaaatc accttgaaaa tcggggaagt aaagtccaac tgtcatcaat tcggcccccg
    19561 aaaatatccg cttcgaggtc tgttcctgat aaagtgcctc atcatctaac cccatcagat
    19621 atacttcatg gaacgctggt tgagctgaag ctagtatcct ccctaaaaac acaatagctt
    19681 catcttgttt aggagacaca aacatccatg cggcaatatt ttcttcaaaa ggactcgcca
    19741 aacgataata atggccatac tggatcaccg gacgaatcgt ttgataggat ataatttggt
    19801 cttttacttg ctttttttct tcaggagaca gctttgtcaa atccagttca taaccaaaaa
    19861 cagcgctcat agctgtttct cctcttgttt tcaacgaagt agatctgtcg gtctgatgat
    19921 ttggtacagc tgatacatga gctgtgatgg acgaaatcgg ataagcaagg cttgttccat
    19981 attggatttt catacgctcc actgcatccg tgttatcact tgtccatgac tgcggcatat
    20041 aataaataaa cccggcatca aaccgtccac ctccaccaga acagccttcc cataagattt
    20101 ccggataatc agttgtcaaa tgttcaagta gttcataaag cccgagcata taacgatgcg
    20161 ctacttctcc ttgtctctgt gggtcaaggg taatcgaata gacatctgaa agcgaacggt
    20221 tcatatccca tttgatgtag tcaagaggaa tcgtatctaa aatctttctc atttgtttct
    20281 caatcgtctg ccgaacatct aaccttgtaa aatccagtac atgttgtgat ctggaagcag
    20341 aaggcattct acctggctcc tgcataagaa aatcaggatg tgttcgatac agttcagaat
    20401 caaccgagat catttcaggt tcaaaccata aaccaaattt caatcctttg ctatgagcat
    20461 aatctgctat ttcacgtaat ccatttgtca acttgccttc atactcgaac cagtctccta
    20521 atgaagaatt gtccgcatct ctttttccaa accagccatc atctaatacg aacatttcga
    20581 tgcctagctc ctttgcttca tcaatgatcg ccttgatttt ttcactattg aagtcaaagt
    20641 aagtggcttc ccagttattg accagaatcg gccgttcagc aaattgatga cgacctcttg
    20701 cgattctttc acgtagcaac tcatggtgga tctgactcat tttgttcaat ccctgacttg
    20761 agtaagaaag gatcatttca ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa
    20821 aacgatggct gttgatccca ccgacgactc tcaattgatt gatctgatct ttttcaagtt
    20881 cgaaagaatg gttgccggaa taaacaaatt gaagtccgat cgcttctcca gtattttctg
    20941 tcgtatgatg atgaactaaa gcaataaagt tattcatatg gtgactgctc gttcctcgac
    21001 ggctttcaaa tacttttgtt ccagataaaa cagattgccg tgaaatctgt ctttcattga
    21061 catgtgctcc tggcaaagca atgacttcat caaatcgcct agtatgggca aagtctagct
    21121 ggaaactagc cgctttttct agtttgattg tttctccggt ttcatttctt atctttacag
    21181 atcgtgtgat cactgctcta tcagtaaata tggtatagaa caaatcgaaa taaatcgctg
    21241 tttctctatc cttaagtgaa atcaccaacg tttgtgcctc tgacttgtcc atcacataag
    21301 tttgaggcaa gccttcgatc tctggcttcc catctgtaat ataatggctg tcatatctca
    21361 gatccagtaa gtctgatcca ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca
    21421 tgctgccatg agaagagtat tcttgcggta gtgtatcttt cgacagagac cggtcaggat
    21481 ttaagggaac atttcctgaa aatccacgat ctcttcgggg ataattttta ttgtctttgt
    21541 actgcttgac tggtttgcca aaataaatat gcgccaatac attgctttct tctatcttta
    21601 tcagataaga aagaaatgta tttcgtaaat ggaagactcg gctcttttca tcaaataaga
    21661 tgaaattttt atctgttttt ctatgcgtaa tatccatcat ttcactccca acaaaacgga
    21721 aaccgtttac ttttttttat aattcctgta tactgtttac taaacaattt tgtcaataaa
    21781 aattttttat ttttttgagg agtgtgtaaa aatggttgga attagagatg tcgcacgaaa
    21841 ggcaaatgtc tctcctgcta cagtttcccg tgtgttgaat aacgatcaga cgatccatgt
    21901 ggctcagact actcggcaac ggatagtaga ggcagctgct gcgttggatt atgacatcag
    21961 tactcgtaaa tatatcaaaa agagaatgcc ttcgattggt gtgatctcta cgatcggcaa
    22021 agcgagcgaa gaacaggata tctattacaa acatttgcga gtagggatgg aagaagaagc
    22081 tcgacgtctg catttaggga tgaatcgaat ctataatcta tctgataatc ctaaacaatg
    22141 ggaagatcta gatcagttag gtgcggtgat cgttgttggg actgttacga aagaatcgat
    22201 taccgccttg ttaaaacaaa acatgcaaat agtcgtcgtg gataaccctg acatccagca
    22261 agaggtcgat atggtgtatg cagatcttga acggatgact aaaaccgtac tggaattgtt
    22321 cttagaaaat ggtcataaaa gaatagccta tatcggggga tataaagtcg atatggatga
    22381 attcggtggg aagcagattt ctataaatga aaagaggatg cgtgcctatc agcattttat
    22441 gatcgatcat cacttagaaa aatatatgtc ttatcatcta ggtggctgga cagaaaaaca
    22501 aggtgaggta cttgcgacta atctattaga aaatgaatcc cctcttccta ctgccattct
    22561 tgtcgggagc gatccaatgg caattggtgt ctatcgggca ttgaaacaaa aaaatatcca
    22621 aattggagaa gagattgttg tagcaagctt tgataatatc gagacaatcg caaacctctc
    22681 ccctggcttg accagtgttc aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat
    22741 ggcattggag cgaattgacg gtatacggga cgaaccaatc attttgactt atcctgcacg
    22801 attgattgtt agggaaagtt ttatcccaag aacaaaaagg tattgaaaga gaggttgtga
    22861 aaagccgctt cgtttcttca tactcaaaac gaaacggctt tttcacaacc tcttacttat
    22921 acttattcac aaaatccaat gtctgctggt aacaattttg cgattctttt agcgtgtaat
    22981 caaattggta ttcatgggta atctcttttt ttgtatctgt ataaaacaag gattgcacgg
    23041 gtacctttaa ttctgtcaac cgattttcta atgcttgacc ttgctcctgg aatgagtatg
    23101 catttccgtc agttatatac gttggcggaa attccttaga aacatggtca gccacactag
    23161 cttctttcaa ttgctcactc gttttccaat cttttgttcc aatcaacgac cacgcaacgg
    23221 ttttgacgaa aaacttcata aatcgatcat ccgaatgctg ctgtgctgtt agcttcaaat
    23281 caacaggtcc ccaataagaa atggttcctt tcagactatc ttttggaagt agctgtttga
    23341 ttttcatttg tttagcatac gcatcattcg tttgtatagc tgcatattgc aatgcgattt
    23401 gtgaacctgc actatctcca ccaaaaaata attgttccat gtctagcaat ttgtcgtctt
    23461 tttcttcagc taataaatct ttgatcagtt cattcacttg aagaacttga ttagggtatt
    23521 ctgaatcagg cgctaattca taattcatcg caaccactgc gatgttggcg tctgaaacta
    23581 attttgtcgc aaattctttg acacccgatt tatctccgcc gacgaatcct ccaccatgta
    23641 cccaaaatag aacaggaaca ggccccttgc tgttttttgg atagtagatg tcataagtat
    23701 tctctttata cttggactca tacgtcaaat ctgttttttt cgccacgttt ttttctgctt
    23761 gatcgaacgt ttttttatca gtgatttgaa cagtatttga aaacatatgg ttgatcaaga
    23821 atgctccagg tctgggggaa agctggaagg caagaaagat tgcaaacgcg atcccaagaa
    23881 tgattccgaa gaaccatccg ataaatttaa gaattttctt tttcataagg ctccctcagt
    23941 agttagtatt aagcattctg attataccat agtgttattt cctatcaagc tctcctagat
    24001 gatttaatga ttttccccaa acttcttctg caatgctttt gacaaaagaa attttttcca
    24061 ttgttcttca aacaaaacat tttcttcttc tgttgaagca aatccacatt gaggacttaa
    24121 gcacaaacga tccaaaggca gatatcgtgt cgcttcgtag atccggtcaa tcacttgctg
    24181 gtgctcttcc atttcgccat ttttcgttac agtcaaaatc taagacaact aatttatctt
    24241 cagagacttt ctctaaaaga acaaaactcc cagcacgttc actatcgtat tctaaataat
    24301 aggcacggac attttcttga gtaaaataat gggtctgcta cgtcgtcatc ccccctgatg
    24361 ccgccaaaat cgaataataa tttccgcgtc aaacatgtat atttagtgtt gaatcatttg
    24421 agaatatgaa aataaaaatc tttttgatca atcagttaat caaattgaaa taacgaaaaa
    24481 tctgagacag aatgacgttc attctgaccc agatttgatt ttgcacaact caacagtcct
    24541 tattggcaaa tcgccaaaat aatgtagact aacta
//
LOCUS       DARBEC010000038        24443 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.038, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000038 DARBEC010000000
VERSION     DARBEC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24443)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24443)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24443
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.038"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(53..901)
                     /locus_tag="SCL80_001411"
     CDS             complement(53..901)
                     /locus_tag="SCL80_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1107288.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(1125..3065)
                     /gene="thrS"
                     /locus_tag="SCL80_001412"
     CDS             complement(1125..3065)
                     /gene="thrS"
                     /locus_tag="SCL80_001412"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEG1107289.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDTDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(3402..3581)
                     /locus_tag="SCL80_001413"
     CDS             complement(3402..3581)
                     /locus_tag="SCL80_001413"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEG1107290.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(3628..5451)
                     /gene="cadA"
                     /locus_tag="SCL80_001414"
     CDS             complement(3628..5451)
                     /gene="cadA"
                     /locus_tag="SCL80_001414"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1107291.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            5729..8008
                     /locus_tag="SCL80_001415"
     CDS             5729..8008
                     /locus_tag="SCL80_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1107292.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(8101..8748)
                     /locus_tag="SCL80_001416"
     CDS             complement(8101..8748)
                     /locus_tag="SCL80_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107293.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(8927..9646)
                     /locus_tag="SCL80_001417"
     CDS             complement(8927..9646)
                     /locus_tag="SCL80_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107294.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(9777..10928)
                     /locus_tag="SCL80_001418"
     CDS             complement(9777..10928)
                     /locus_tag="SCL80_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEG1107295.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(11145..11885)
                     /locus_tag="SCL80_001419"
     CDS             complement(11145..11885)
                     /locus_tag="SCL80_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1107296.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(11889..12212)
                     /gene="rsfS"
                     /locus_tag="SCL80_001420"
     CDS             complement(11889..12212)
                     /gene="rsfS"
                     /locus_tag="SCL80_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEG1107297.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(12269..12862)
                     /gene="yqeK"
                     /locus_tag="SCL80_001421"
     CDS             complement(12269..12862)
                     /gene="yqeK"
                     /locus_tag="SCL80_001421"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEG1107298.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(12846..13496)
                     /locus_tag="SCL80_001422"
     CDS             complement(12846..13496)
                     /locus_tag="SCL80_001422"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEG1107299.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(13506..13817)
                     /gene="yhbY"
                     /locus_tag="SCL80_001423"
     CDS             complement(13506..13817)
                     /gene="yhbY"
                     /locus_tag="SCL80_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEG1107300.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(13833..14942)
                     /gene="yqeH"
                     /locus_tag="SCL80_001424"
     CDS             complement(13833..14942)
                     /gene="yqeH"
                     /locus_tag="SCL80_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEG1107301.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(14939..15466)
                     /locus_tag="SCL80_001425"
     CDS             complement(14939..15466)
                     /locus_tag="SCL80_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEG1107302.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(15646..16431)
                     /locus_tag="SCL80_001426"
     CDS             complement(15646..16431)
                     /locus_tag="SCL80_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEG1107303.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(16424..17293)
                     /locus_tag="SCL80_001427"
     CDS             complement(16424..17293)
                     /locus_tag="SCL80_001427"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEG1107304.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(17294..18673)
                     /locus_tag="SCL80_001428"
     CDS             complement(17294..18673)
                     /locus_tag="SCL80_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEG1107305.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(18681..19100)
                     /gene="fabZ"
                     /locus_tag="SCL80_001429"
     CDS             complement(18681..19100)
                     /gene="fabZ"
                     /locus_tag="SCL80_001429"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEG1107306.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(19125..19601)
                     /gene="accB"
                     /locus_tag="SCL80_001430"
     CDS             complement(19125..19601)
                     /gene="accB"
                     /locus_tag="SCL80_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEG1107307.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(19604..20839)
                     /gene="fabF"
                     /locus_tag="SCL80_001431"
     CDS             complement(19604..20839)
                     /gene="fabF"
                     /locus_tag="SCL80_001431"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEG1107308.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(20858..21595)
                     /gene="fabG"
                     /locus_tag="SCL80_001432"
     CDS             complement(20858..21595)
                     /gene="fabG"
                     /locus_tag="SCL80_001432"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEG1107309.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(21607..22524)
                     /gene="fabD"
                     /locus_tag="SCL80_001433"
     CDS             complement(21607..22524)
                     /gene="fabD"
                     /locus_tag="SCL80_001433"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEG1107310.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(22631..22855)
                     /locus_tag="SCL80_001434"
     CDS             complement(22631..22855)
                     /locus_tag="SCL80_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEG1107311.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(22908..23870)
                     /locus_tag="SCL80_001435"
     CDS             complement(22908..23870)
                     /locus_tag="SCL80_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEG1107312.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(23880..24320)
                     /locus_tag="SCL80_001436"
     CDS             complement(23880..24320)
                     /locus_tag="SCL80_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1107313.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
BASE COUNT         6691 a         5436 c         4253 g         8063 t
ORIGIN      
        1 gaaaactacg ttttgttatt ctaaaaaact tatataaaaa atagtaaggt ttttatttcg
       61 aatccataaa atattcttgg attgcttctc gtgttttttt cactttttcg ctaagctttt
      121 cgtttttagc catatagaaa taaaaaatga tatcgatcgt agcaaattgg gccaaaagtg
      181 aattagttgc cgcacttcga ttcgtgattt cttttggtct agatgtttgt acgagtacat
      241 cggctatctt gctaagcggg ttatttccaa attgcgtcaa cgcaatgatt tcgatattca
      301 tgcttttcgc cagctcggct aatgcgacta catctgcact tctacctgag tttgaaacaa
      361 gccaaagcac tttctttttc tcattgctga caagttgcgg gagcaagacg tggatatctt
      421 tttcaaaaat gattggctta ccgacacgag accatttttg cagaatatct tctactacta
      481 aagaagaggc gccaacccca tataaaaaaa cagtctctgc cgattccaaa cactctacga
      541 ctttttggat cgtttcttct tctagaatat ctactgtttc ttttatcgct acttgtgcat
      601 tgttaccaag tttgtttttg atagagtaaa aggactcatt tgcttctata tcactaaaac
      661 cttcatcgat tccagtctgt agttctgttg aaatcgtgat tttgaagtcc gtcagacttt
      721 gaaaaccgat ttttttggaa aaacgtacaa ctgtcggcgc actcaacccg gaagcttctg
      781 ctaattcatt agctgtcatt gtagggacct tctcaatatt accgataatg tagttggcca
      841 actttttttc tgctgaagaa taatcttcca tcatactttc aatacgacta attacgctca
      901 taagttcctc caccctttta atgatagtct atgtaagtat accatatcag acccttattc
      961 acggtcgtat actggataaa aaacaaaaaa acaatccgcc caacagaata tcttatccca
     1021 tcaatcccac agattctatt gagcatctgt gtctttgagg aatacctgtt atctattgtt
     1081 cggattgctt tctatctact acttttttac aggaaattta cctattagtt ttctctgctg
     1141 taatttttga cttctgcttc catagcatca ataaagatat tcaaatcttc tacagctgtt
     1201 tctttacttc cataacgacg tacgttcact gttgcatctt ccaattcctt gtctccaaca
     1261 actaattggt aagggatctt ttgtgtttga gaggcacgta tcttatagcc cattttttca
     1321 ttacgatcat caacttctac acgcaacccc ttcatttgca aacgttcttt gatttcataa
     1381 gcataatcgc catgtgcatc aacagaaaca gggatgattg ttgcttgtat aggtgccaac
     1441 catgttggaa aagcgccttt gtatacttct gttaaataag cgacaaaacg ttccattgta
     1501 gaaacaattc cgcggtggat aactactgga cgatgcgtat tttccccgtc ttctccaaca
     1561 tatgtcaaat caaagcgctc tggcaataag aagtctaatt gtatagttga taatgtttct
     1621 tctgtaccaa gagctgtttt gacttgaaca tctagttttg gtccgtaaaa ggctgcctcg
     1681 ccttctgctt caaagtaatc tagttctaat tcatccatag cggctttcag cattgcttgg
     1741 gctttttccc acattgcgtc atcatcaaaa tatttatctg tattgtttgg atcgcgatag
     1801 ctcaagcgga aacgataatc tgtaatattg aagtcagcat atactgccac catcaattca
     1861 agcgtacgtt taaattcatc tttgatttga tcaggacgaa caaatgtatg accatcattc
     1921 aacgtcattt ctcgtacacg ttgtaaacct gacaaagcac ctgatttttc atagcgatgc
     1981 atcattccta attcagcgat acgaattggt aattcacgat aagaatggat cgtgtttttg
     2041 tagaccatca tgtgatgagg gcagttcatt gggcgaagaa caagcatttc gccatctccc
     2101 atatccattg gcgggaacat gtcttcttga taatgatccc agtgtccaga tgttttgtac
     2161 aactcaacat ctcccattat tggtgtatat acatgttggt aacctagact aacttctttg
     2221 tctacgatat aacgttcgat cgtgcgtcgg attgttgccc cttttggaag ccagaatggc
     2281 aaaccagaac ctacttctgg ggaaaccatg aacaagtcaa gttcttttcc tagtttacga
     2341 tggtctcttt cttttgcttc ttcacggaga cggatatatt ctttcaaagc tttcttgtca
     2401 aaaaaggctg tcccgtatac acgttgcatc atttgattgt ttgagtttcc acgccaataa
     2461 gcacctgcca ctgataataa tttaaatact tgtatacggc cagtggatgg aacatgtggt
     2521 ccacgacaca aatcgatgaa ttcgccttgt tgataagcag taatcacttc ttcttctggc
     2581 aattcactaa tcagttctac tttgtacgga tcacttgcaa aaatttctaa tgcttcctgt
     2641 tttgaaagaa cacggcgttc gatcggcaga ttttctttga cgattttcat catttctgct
     2701 tcgattgccg gcaaatcttc tgcagttact tggttttgtc cattatctgt atcgtagtaa
     2761 aaccctgaat cgatagctgg tccaacgcca aagtggatat ttggatagag gcgacgcatg
     2821 gcttgggcca tcaaatgagc tgctgaatga cggactaagc caagtgcatc ttcatggtct
     2881 ggtgtgatga tctcaatact tgcatcttcc tcgattggac gagtcaaatc aacgagtttt
     2941 ccgttcactt taccagctaa agcttttttt gctaagcttt ttgaaatgct ttcagcaatt
     3001 gccaatgttg ttgttccaga ctcaaattct ttgactgcac catctggaaa agtaattttt
     3061 atcataatag tttcctcctt gaaatgtttt gattgatcag atcatcaagc tgaaaaacaa
     3121 aaaaatccta gcatctcttg aaaagagata ctaggacgaa aagttcgtgg ttccacctaa
     3181 tgttcaaacg caaatgcgtt cctcaagcgt tgtaacggtc cgaccgccct tgcttcaaca
     3241 agggtttcga aagtggtcat cctttggttt tctcctggaa aagtttcagc ctagcttttc
     3301 ctctcttatc gaagactctc aaattcagtt ggctttctca tcaatcaatt atgtgtttat
     3361 ttaaccacta ataaatagaa aaatcaagtg ggaaactaaa cttatttttt ccattcccca
     3421 tttacaccgt atgttccttt tttcatctca ttgatgatga catggatgtg ttcttttggt
     3481 gcctgcgtgt ttttatgaac agcttccgtg atatctttca tcatattttc taattgttcc
     3541 ggactgcgac cttctactaa ttcgacatgt acgaatggca taatgctctc tcctctttct
     3601 gatcgttctt ttttcaactc gattctatca tactatgttt gtcttgggta ctgaatcagg
     3661 aaaaatggct ggatcttttt ttgctctcaa ggcactcaaa atagagaccg tatccacgac
     3721 ttcttgcaaa acagctccga ataatgcggg gattagtccc gtacttgcaa ttaacatcaa
     3781 aaaaatacaa ataaagatgc caatcagtac agactgtttc gctattctca tcgtatctct
     3841 tgaaatcact actgcttttg ctacacgcaa gagatcatct ttcaagatga ccgcatctgc
     3901 actttcacta gcagctgtag aaccatgagc ccccattgca ataccgatat ctgcagcagc
     3961 aagtgctggt gcgtcattca cgccgtctcc taccatcgca acaggtcttt gatctttagg
     4021 gatactttct aaaatggtca gtttatcttg aggcaagcta cgggcatgta cagaatctaa
     4081 ttggactttt tctgcaatcg tttgagcaat ggcagatcca tccccggtga tcatcatcaa
     4141 atttttcaca tcaagttttc ttagctcctt gattgtcttt gctgcttctg ggcgaatctg
     4201 atccgaaaat aggatcgttc ctgcaaaaga tcgatcaatt gttacataga ttgctgtatt
     4261 ggacgtttct atttcttcag ctgaaacaaa agaggcacgt ccaatcttta cttcatgatt
     4321 attgactact gcttggattc cttgaccggt cacttctttc agttgactaa caggatacag
     4381 ttggctgttt ttcacgttgt tcactaacga acgagctaag atatggctag attcctgttc
     4441 tgcactagct gcatacagaa gtagctcttc tttttttatt ccattgactg gctgaatcgc
     4501 ctctacttcc aatatccctt tggtaagcgt tcctgtttta tcaaaagcaa tcgtttttgt
     4561 acgtgcgagc ttttcaatcg ttgttccagt ttttacaacg atcccgtttt tacttgaacg
     4621 gctcattcct gcaactaaag caatgggcgc agctagaatc agtggacaag gtgatgccac
     4681 taccagtacc tcagcaaatc gtaaagggtc ttttgagaac caccaagaaa taccaccgat
     4741 caaataagca atcaacgtga aagggactgc ataacgatca gctaaacgga caaatcgggc
     4801 aggcttttct tttgattctt cgactagttt aataatcatt tgatactggc tgtctgcggc
     4861 acgtttttca gcgatcattt ggatcgatcc gtctccattt aaagaccctg acataatctg
     4921 atctccaggt ttcttggttt ccgggcggga ctcacctgtc aaagaagatt catcgaccga
     4981 agattctcct ttggtcactc gaccatctgc gggtacaagt tcgccaggct tgactagtaa
     5041 ctggtcatca atatttattt catcgattac tatatcctgc aattttcctt ctttgaatcg
     5101 atgggcaatt cttggggtgt tatctaacaa agactgcagt tctttacccg ctcgtttatt
     5161 cgcgtaatcc tctagactat ctccgcccgt aagcatgatc aatatcatca aactagccca
     5221 gtattctttg acggctaatg ttgcaaggat tgcggtaatc gctaatacat ctacgccata
     5281 tcttcctgaa cgtaacgtct tgatcatttc caaaaacatc aagaaggcga gaatccctcc
     5341 agtaaatgcg acaatcgcaa atgcaagggc cggctggtga aaaacaaatt cacttaataa
     5401 agctatccca ccaataagca aagtaagtaa aaattttttg aaatgtgtca ttgttttttc
     5461 tcctctttta tgtgacttag agaggttagt aataaatttc cagctgcctc tttcatctta
     5521 agcatagtcc ttgactagca ctttttaaaa caataggcct taattgaaaa caactatcac
     5581 tatcagcgtt tccaaaattc atcagttcta caaacaaaaa tattcagcaa ggattacttt
     5641 ttcgattcat cagttaaaaa gtaccttttt tcgaaaaact atgctatact tttaattcgt
     5701 tatttacggc ggaaggagca aacatgcgat gaatgagaaa caactagaac aaacgcacgt
     5761 ggatgaaacg atcgaactaa tccagatcga acaaaatatg ctggatacaa aacaacaaga
     5821 attgactcag gaaatgaatc attcgacaaa agacacagct aatcacaaga ttcgagctgg
     5881 ttccaatgaa gctttttatg aatctgctgt tgaatatcgc caacatgagc aggaattgct
     5941 tttgaaatac catactcttg aagcacagca aaaacgcctg caaacgttgt ctgtcatgaa
     6001 agatagtcct tattttgccc gtatcgactt taaagaagac cgtgaaacag aaacattata
     6061 tctaggtatc gcttctttaa gagatctggc agaagaaaca atcgttattg actggcgcgc
     6121 accaattgcc aatctttatt atgaaggtga gattggtccc gcattttatg aaactgaaac
     6181 agatagaata gatgtcgatc tgcagttgaa acgacaattt aaaatccaag aaggacgtat
     6241 tttatctatg gtagatacgt cggaagtaat caatgatgac tttttgctag agatattgga
     6301 tgaatcatca agcaaccata tgaaaaacat cgtctctacg atacaaaaag cacaaaatgc
     6361 aatcattcgg gacacaacaa gtaaagtaat gctgattgaa ggaatcgccg gaagtggaaa
     6421 gacatccgct ttgcttcaac gtattgcttt tctgttgtat cataatcgca agtggctcga
     6481 ggctgaaaac gttcttctat tttcacccaa ccatttgttt tctgattaca tctcgactgt
     6541 attgccttca ttaggtgaaa gcggcgtgcc tacacagaca ttcaaaaatt atttggtgca
     6601 gctgctgcca gagttttctt tactagatga acagcaacaa gaaagtggtt ttttgctagg
     6661 ggaaaaagat ccgatacaag tgatgaaaag cggattgacc ttagtcgatc agattgatcg
     6721 ctatatccaa ggcatcacaa actacggtcc cttgttccgt gatatgaaaa taaatggccg
     6781 tacgatccta tcaaaggaat cgattcgcca atggtataaa gaaacaaatg aacaattacc
     6841 tttacatcat agactttctc tattacaaac gaagctcctg aaaaaattag gcggtctgca
     6901 aaaagacgaa acacgtcagc aatgggtcaa agatctggcg gaagaacagc tccaagaact
     6961 atacgcaaca gatccaaatc ttgaatatac agaacaaaag gagcgttcat tacgtaaaaa
     7021 actggccaac caaatcgtac gtaaaagatt ccgtcgaatc catcgaggga tcatgaatta
     7081 tcaatttgtc aatataacga aacaatatct ccactttcta caaactgtgc cactttctct
     7141 tctaacacga cataaaatca gcgagaccag gtggcaggaa catttgacaa ctgttcgtac
     7201 acacttgcgt caaagagaat tgcgtcaaga ggatgctgta ctattctttt tactgatgcg
     7261 ccgaacatat ccagtgacgg ttacacacaa agctcgctat atctttattg atgaaatgca
     7321 ggatttcgca ccatcacaag tggcattgct gcaagccttg ttcccaaaag caaatttgac
     7381 tttctgtgga gatctaaatc aaaatgtctt tggaaatgaa acgatcgttg gatcattgga
     7441 agaccttttc gtggaccgtg aagtcactcg tttccaatta acgacaagct atcgctccac
     7501 aaaagaaatc actgattttg ctaatcattt tattgtgaaa gaacatcaag tagaaacaac
     7561 agcaagaaaa ggagaccttc ctttgctcat ccaaggaaca acagatgata tcaaattgaa
     7621 ttggttagca gataccattc gaacgactca gggaaaagcc cggtattggc gtacagcaat
     7681 catagctaaa acacaagaag aatgcgaagc tctttatgct caactgccag acgagttgaa
     7741 acaacaagtc cagctgattt taaatgagag tgactttatg aagcgttcca ttattttact
     7801 tccagccttt ttagcaaaag gtctggagtt tgaccgagtt ttcttgtggg atgtagatgc
     7861 agaacatttt tcttctcagc aagacaaatt gatcctttac accatgtgta cacgtgcgat
     7921 gcatgaattg acccttttta cctccaataa tgaaagtcct ttgttaaaca atatggatat
     7981 ttcatcttat gagaaaaaag tactgtaagt aaaaagacaa ctgctatcat taatgttagc
     8041 agttgtcttt ttatttacaa taataattgc agaaaaagta attcaatcat ttaaggtcat
     8101 ttattctata tataaatcca aatctgtttc aggatatttt aatttgacaa gcgaaataat
     8161 cattttcagt tcttccatgt tttctggata ttctgggtca aataccttgc tatgacggcc
     8221 aatcccgcca ttgtcgatca atcggtaaat tcgattccat tcaatgaatt ggtcaattga
     8281 ttggaaattg ctgattggat agccgccaca atccaaaatc tctgatacac gaaatgctag
     8341 gtatgcacta tcgatcactt tatttttttt cgtgtctatc ttctcatttc tttcgataag
     8401 aattttttca ggacgatgtt tcaagctcca ctctaatagt agaaagaaag cctccatccg
     8461 atactcttct tcaaataacc gaaaggctga atcagaacga cgaaaaagca gcggaatcag
     8521 ataattttgt ctataaatca gcattggtgc gcggtccacc atacactcta aataacggtt
     8581 cagataccct gtaccgacat agtttcctaa tcgatagcct tcaacgccga atcttgcgta
     8641 tttttgcgct aattttgaac aatcagttac gttcacagct aactcttcta ctttgcggta
     8701 accgcttaag atacgttcaa aaggttctaa agtgattttt gcttccatat tcttccccgt
     8761 ccatcttcca catacttcat atcatttcac atgatactta tgctctcatc ataccacagg
     8821 ttgaattaga aaagaaaata aatcacacaa aaaagcaaga caatctgtta gtgagattgt
     8881 cttgcttttc gaaaaagact gaatactttt cgttaaaact ggttgcttag tcttctaatg
     8941 ccacattatg gaacacatgc tgtacatctt ctagatcttc cagtgcatca atcatttttt
     9001 caaatgtttc ttgatcttct ggagatagtg ttacctcgtt ttgagggatc atttggatct
     9061 cagcgattga aaaatcagtg attccttttt cttttaatgc ttcttgtata ctatggaaat
     9121 ccgctacttc accgtaaacg atgatttgtc cttcttcttc cattacatcg cgaacatcga
     9181 tgtctttttc cattaggtat tcaaggatct cgtcagcgtc ttctcctgca aaagcaaaga
     9241 ttgctgtatt gtcaaacatg taagaaaccg ctccggaaac acccatgttc ccgccatttt
     9301 tcccaaatgc tgcccgtacg tctgctgctg ttcgattcac attgttcgtc aatgtatcaa
     9361 caatcaccat tgagccgctt ggtccaaatc cttcatagcg caattcagag tatgtttcat
     9421 ctcctgcacc ttttgctttt tcgattgctc ggtcaataat atgttttggt acattataag
     9481 ttttagcgcg atcaatgacg aaacgtagtt tttggtttgc atgcggatca ggatcacctg
     9541 attttgctgc cgcgtagatt tcaatcccaa attttgcata aatccggcta ttattagcat
     9601 ctttagcagt tttctttgct acgatatttg cccatttacg tcccatattt tcacttcgct
     9661 ttctgatttg atagtttcat aacgcaaaaa cgtcctgttc cattatactt gtgaatcacg
     9721 attctgtgaa gctttttttc tttcttcctt cattcttctc atttttagtc aattatctat
     9781 actcggattg ggatttgccc gaaattctgt tccggaatat tgcctcccaa ttgatagatt
     9841 tgatcacttc taactaacag ttccgcttct tgtgcctctt tttttccgat tcgggaataa
     9901 atagaaatct cttttttttc ttttaagaat cttcttccag catcggtaaa tccaagtagt
     9961 cgaagataat ttttattttg ttggacagga atctcttgct ttttgatatt cagcaagaca
    10021 taacaagata atcgttggat ccgtgtccat gtatatcttt ttgtttttac ctttgacaca
    10081 aaatcctcaa aactatcagc agttgcagct gctgttttca tgcgcgattc tattccttcg
    10141 gtcatctgat agatatctgt cagttccagc aaagtagaag ataacaaccg ataacgcaaa
    10201 tacggccaat aagattccca agtctgcagc gaacaagttg aaagatcttc ttctgtctga
    10261 ggcggcacaa gtccagctat tctttttcct tcttctaacg catggcgaat ggctgttgca
    10321 ctggctatcc tctgatctgt gatgattttt tcatgatagc ctgcactttt acgtcggatc
    10381 ggatgtaatt ccatagggtg gacatacttg gcattttcct ttgcataact taatcccaag
    10441 atatgatttg gcgaagaaaa atcaagcgta atatcgggat acagactgcg aaaaacctca
    10501 gtcatttttt gcggataact atacgtttga tcagacagtt cttgaaatgc ctcttctatc
    10561 ctatcttgat tctcttggac aaagcgtccg aaactgctgt aatcaaacga agtctcttct
    10621 tctgttccaa aacacaaact gtcacagtcc agtgtttgca agatttttac tgcaccttta
    10681 gcaaaatagt cagctgactg aaccgcccat tcgacgggaa gctccactac taaatccaca
    10741 ccattttgta aagcttcatt cgcgcgatgc catttatcga tgatagccgg ttcgcctctt
    10801 tgcaaaaaat tcccgctcat tacagcaatg acgacctctg ctcctgttat ttttcgtgcc
    10861 atttccacat gatagcgatg tccattgtga aaaggattgt actctacgat aatcccgcag
    10921 cttttcatgc gcatcctcct tcatagttat cgataaaaaa gaaatagaga caagtaaaaa
    10981 aaggcaaaaa tcggaaaaaa tacccgaact atataaacta ttctcgcaaa cacaatactt
    11041 ctgcgacgaa aagctgcact gcaagagcaa tgcctatatc gttcggatat ataatttatg
    11101 ttgaagattt attcttttct tgttaactcg agcaggcaaa accgctagtc tttgtatgct
    11161 acaaagaacc agcgctttga tttctctgat gctgcttcgt ctgtaaaatc agaaaatgct
    11221 tcgactttcg aaaagccgct gttttccagc attcttaaat aactttccaa tggataggta
    11281 cgctcttgat gtagttcatc ttcacgaata aatcgaccag acccttcttg ttcttcaacg
    11341 aaaaaggtaa gaaaatgttc gatgctgtgt ttctcgcttc ctggataact atcccacaaa
    11401 aaagcaaaat tatccccttg ataatgatag ctatattctg gaaaaacctc atctacttga
    11461 taggtggagt gtacgtcaaa taaaaatcgg ccattttctt ctaacgcttg atagacttca
    11521 tcaaacactt gctgtacctc ttgtctattt ttcatatagc acagcgaatc agaaaaacag
    11581 gtaattgcct gatattgtcc gatttctgat aaatcgagca tattgccttc cacaaattgg
    11641 atcgatgcgt cttcttcata tgctcgctgg ctggcaatca tcaacatctc ttctgaaagg
    11701 tctaaccctg tcactttata tccatctttt gcaaaggcaa ccgctaatgc tccagtaccg
    11761 catgccagtt ccaaaatatt ttttgtgtct tcttgtaaat gacgtttcga aaaagctaac
    11821 cattgctgat aaagtgtttc gtccatcact tcatcgtaaa tgaaagcaaa tgtttcataa
    11881 gccatatgtt aatccaacca ttcactaaga tcgactagcg gagcatctga ccatagtttt
    11941 tccagattat agaaatttcg ttctggcgca tggaaaacat gtacgatcac atctccaaga
    12001 tcgatcaata cccattttcc gccttctttt ccttcaatac gttttacttc atatttgttt
    12061 tcttcttctt tatcaatgat ttcttctgta attgcgttga tttgacgttc gctatttgct
    12121 gaacagatca tgaaataatc tgccaacaag gaaacatttc gaacgtctaa agcgataatg
    12181 tcttctgccc gttttgaatc tgctgcttca atcgcaattt ttaattgttc tttactatct
    12241 atgatgattt cctcctaata tgagcagctt atttttttgc tacccagtaa ttatatgttt
    12301 cgattgtttt tggataaatc tgttgttctt gcgcaagcaa atgggccaat gtatgttttg
    12361 tttcaaatgc tacagcttcg tctagatcag cccatgcaat ggctcgtgca tcttgtaccc
    12421 caggaaaatc tcgacctggt tcaatatagt ctgctacata aatgattttg tcaaggagac
    12481 tcatcttagc agccccagtc gtatgcaccc ggattgcatg aagaatctct gcatcggtga
    12541 ttcctaattc ccgctctaca aacgaagcgc caacaagacc atgccagatt gcattattat
    12601 aattcaataa ctccggatca aaaccgtctc ttacgattac cattttgaac tcctcatctg
    12661 gtcgttcttt tgcataatca tgtgtcaatg cagcaatact agctttttca ggagaagcac
    12721 cgtatttttt ggcaagagcc acggctgttt cttctactcc aagcacatgc ttgaagcgtt
    12781 gttcgctcat tctcatctgt attttttgca tcaggttttc tctagaataa gcagtatatt
    12841 cattactata gtccatattc atacaaccct tcattttgaa tataatcaat tactttatct
    12901 ggaacaaggt agcggataga acagccttct ttgatctttt gacgtatttt cgtggaactg
    12961 atgtcgatct caggtacatc tacccaaatt acaggatatg gagtatccgt tgtgtatcct
    13021 ggtcgacgta tacccacaaa gttgaccata gaagtcagct catcgatttt ataccatttt
    13081 ggcaaatatt ctaccatatc cccaccgata ataaagtaat aatctgtatc cggattgttt
    13141 tgcgtcaatt ccttcatagt atcataggtg taacttttcc caccacgagc taattcaatc
    13201 gtttcaatct gcaaaaaagg attgtcttcc accgctaatt ccaacatgtt gagtcgatgt
    13261 tttgcatcaa tcgtctgttt ctcatcaaca tgaggtggct gataagaagg catcaaaaat
    13321 acgcggtcaa gacctaaatt ccggccagct tgttcagcca tcactaaatg tgcaaggtga
    13381 acgggattga atgttccgcc caagatccct acttgctttt tcttctctaa gaattgaggc
    13441 atttcttcag gaaataattg tgaacgaaca aatgtcttag cttgcgtatt catcatggtc
    13501 actccttata ttgcttttac ttcttttgat agcctttgat acttttcttt tgtggaaggt
    13561 ttgtataata caataacacg tccaatgatt tggacgattt cgcaatctat ttctttttct
    13621 aatacttgag ctgcttcttc tgcaatctca tctgtatttt gaagtaacga tactttgatc
    13681 agttctcttt tttctaaagc ttcttcaatt tgaacgatca ttgctgaatt gatcccacct
    13741 tttccgattt gaaaaatcgg ctgaagatga tgcgctttgc tacgtaaaaa acgcttttgt
    13801 tttcctctta aactcataat tttccttctt ccttaaatca atgctttgcg tttcaataca
    13861 tctacaccct ttggcgccca gcccgcaatt actactggcg ctgttacagt gatccagcct
    13921 agaccggcaa aaacgatgtc tgttttttct ttcacagaaa actcaaaacg aacaagctcc
    13981 ggaaatgcct cgacttcatc aggacgagga ggctgtaata atccacctac atgtttttga
    14041 taaaattcgt ctgctttttc cagtttcgtt cgatgaatat ttacatcatt tgacacataa
    14101 gcaataaatg aagcacgatt gccgctgata aaatcaaatc ttgcaagtcc gcccaagaaa
    14161 agtgtttgcc cttcattcag ctgataaact ttaggtttga tttcttttaa cggtgcaatg
    14221 atccgcaaat cttttttacc taagtagtgc gccatttgat ggcgatggat aattcccggt
    14281 gtatcaatca aaaaatgccc gtcatctaaa ggaatctcga ttttatccaa ggtcgttcct
    14341 ggaaactgcg aagttgtgat tacatcttgt actcccgctg tatttttgat gatttgattg
    14401 ataagcgtcg attttccgac atttgttact ccgacgatat agacatcccg tccatcgcgg
    14461 tattcttcga ttttttctaa tagttccgtc atctcatgag atttcttagc actagttaat
    14521 aatacatcta ctggacgaat tcctgcttcg taagcacgtt ccttcatcca ttgtgttaat
    14581 ttagtttttt tcaatgattt aggtaatata tccactttgt tcccaactaa aagtactgga
    14641 ttgtcaccga caaaacggtg tagtcctggg attagtgaac cattgaagtc aaaaatatcc
    14701 accacattga cgatcaatgc atctttttgc cctaattcat tcagtaagcg caaaaagtcg
    14761 tcatccgtca atgagacatc ttgggtctca ttataatggc gtaatctgaa acaacgctga
    14821 caatagactt ccccggaagc taaacctttt tccaaagccg tttgcggcgt gtatcctaat
    14881 tcaccgggtt tgtttgtttg tataacggca ccacagccga tacattgtaa ttcttctgtc
    14941 attcgattcc acctcgccag cccatatcag ggtgttttgc taatagatag gccataattc
    15001 ttttttccca aaaacgatta aattgtgtct tccaactatc tgttgcgata atgggtttta
    15061 caaggatgct acgtatccct gcacgattgg ctccacgtat atcagtcata atctgatcgc
    15121 ctaccattac tatctcatca tctgctaaat ccaattgttt ctttgctatg ttgatccctc
    15181 ttgctaaagg tttcattgct cgtgcaacat acaaaagatc aaatttttca atcgctcgcg
    15241 cgacacgttt tgaattattg tttgaaacaa caacaacagg gattcctgca tttttcattt
    15301 ctaaaatcca agtaagcaac tcttctgttc catctggatt attccatgca atcaacgtat
    15361 tgtctaaatc ggtcaatact gctttgatgc ctagattctt taattgggca ggtgtaatct
    15421 gatagatggc ttctaccatc catgttggtt tgtaatttga aaacatgctg tctcctcatc
    15481 tgcaaaaaat gaaggagcac ataaagtact cctcaacccc gtattatact gcatatcagc
    15541 aaaaaaaact acctctatac tcgttcgtct ttcagaaaaa gcatttcttt acaaaatgac
    15601 caaaaataaa gattgatcac agaaaaattt agaaacaaaa aacaactaat attggcggaa
    15661 cctcttgtat cgtttttcca gtaatttatg ctgatctaat cgaacaagtt ccgtcaatct
    15721 ttcaatcaac gcttttttca taatgtgtgc gactttatat cgtggcaact gctcgccgtt
    15781 gaacgtttca ggaataactc gatcaacgat tcctaactcg cgtagttctg gcgcagtaat
    15841 tttcatcaac tcagctgctt ctgcggcgcg actaccgtct ttccacaaaa tcgacgcaaa
    15901 tccttctgga gaaagcacag cataaatcgt ttcttccagc atccagactt catccgctaa
    15961 acctagagca agggcaccgc cacttccacc ttcaccaatg atgatggcga tgatcggtac
    16021 ctttaaatca gacatttcga gcagatttct ggcaatagcc tctccttcgc cgcgctcttc
    16081 tgctcctaca ccgcaatatg cacctgctgt attgatcaag gtaatcaccg gtctattgaa
    16141 cttttccgct tgtttcatca gtcgtaaagc ttttcgatag ccttcaggat ggggagaacc
    16201 aaaatttctg cggatatttt cctgcagatc ccgtcctttt tgaataccga tcactgttac
    16261 cggtttgcca tctaatcgag cgattccacc aacaatcgct tcgtcatctg caaataaacg
    16321 atcaccatgg cattcctcga aagaatcgaa gatctcttca aaaaaatcca agcttgtcca
    16381 tcgttcttta gctcttgcta actgaacgac atcatgtgct gtcttatcca cgatctatcc
    16441 atcctttctg tgtatgcaat tcaagaagcg tatggatttt ctgtttcaat tccatgcggg
    16501 gaacaatctg atcgacaaaa ccatgagata ataagaattc agccttttgg aaatcttctg
    16561 gaagttcctg cttgatcgtc tgttcgatga cgcgtcttcc agcaaaaccg atcaagcttt
    16621 ggggttctgc tagaataata tcgccttcca ttgcaaaact ggcagttact cctccggtcg
    16681 tcgggtccgt taaaacagtt agataaaaaa gaccttcatt gctatgccgt ttgacagcag
    16741 cagagatttt tgccatctgc attagagaaa agattccttc ttgcatccgt gcgccacctg
    16801 aagctgtgaa caagacgact ggaagacttt cctttgttgc tcgttcgaat aatctggtga
    16861 tcttttctcc gacaatcgtt cccatacttc ccatgataaa attaggatcc atcactccaa
    16921 taatgaaggg aatcccctga atggttgctt taccagtgag gactgcttca tgaagtcctg
    16981 ttttatcttg cattttttta attttttccg tatattcggg aaatgctagc ggatctttgg
    17041 ttacaagatc cgtatcccat tcttcaaaac ttttttcatc aataatcaaa gctagacgct
    17101 gccatgcacc gattcgaaaa gcgtatccgc aatgagggca tactttttct tcaccgatgt
    17161 ctttcgtata aaggatatgt ttgcaatttg gacactttgc ccacatgtta tcaggaacag
    17221 atggcgagtt agcaggtttg ctctctctat taggattgat ccgaatatat ttctttttct
    17281 taaacagagc catctatgcg cctcctatct ccgtttctgg tgtccaattc ggtaaaaatt
    17341 cttcttgtaa aaatgctgtg ctgtaatcac ctgctatgac tgcaggatga ctaatcaaat
    17401 ccatctgaaa ttctgcgttc gtgatgattc cttctgtgat aaattcactt aaggctcgct
    17461 gcatcttcat taatgcttca aaacgcgtat ttccatggac gattattttt gcaatcattg
    17521 aatcataata tggcgggatc gtatatcccg ggtaaactgc actgtctact cgcagtccca
    17581 tcccacctgc tggaagcagt aagttttgga tcttccctgg agaaggagca aaatggaaag
    17641 caggattttc tgcattaatc cggcattcga ttgcgtgtcc ttggaatact acttcttctt
    17701 gacacacatt caaaggctca ccggctgcta tctccacctg tttttttact aaatcgattc
    17761 ctgtgaccat ttctgttact ggatgttcca cttggattcg agtattcatt tccatgaaat
    17821 aaaaatcgcc ttcttcatcc atcagaaact cgatcgttcc ggcattttca taattgactg
    17881 ctttagctgc tcgaacagca gcgtctccta gtgcttctct ttttgtttgt gagatcacta
    17941 cagaaggaga ttcttccagt actttttgat tgttgcgttg taaagaacaa tctcgctcgc
    18001 ctaaatgaat gacatgacca aatcgatcac ctaagatctg aacttctata tggcgagcag
    18061 gataaatgat tttttccaaa tacatatcat catttccaaa tgccgctgct gcttcttgct
    18121 gagctgaaga aaaatgctgt gggagctctt cttttgattg aactttccga atgcctttcc
    18181 cgccaccacc tgcagctgct ttcagcatca ctggataacc aattctgtct gctatttcca
    18241 atgcttcttc tactgtagat aatacaccag tacttcctgg aatgactggg acatttgctt
    18301 cttgcatcaa ttgacgcgca ttgatcttat ttcccattgc atcgatcgtt gcacttttag
    18361 gaccaataaa tgtgatattg cactcttcac acatggaggc aaatcggcta ttttcagata
    18421 aaaaaccaaa tcctggatga attgcttcgg cttttgtcac gattgcagcg ctcagtactt
    18481 cctgaacatt cagatatgaa tcaacggctt ttgccgggcc aatgcagatt gcttcatctg
    18541 ctaactgtgt atgaagtgct tcggcatctg cttctgaata tatcgctacg gtttggatcc
    18601 ctagttcgcg gcatgcacga atgatacgta cagcgatttc accgcggtta gctatcaata
    18661 ctttagaaaa catatttcac ctatccaatc ataaaggtta attccgcttc ggctactttt
    18721 ttaccgtcta ctttagcgat tcctttgcct attccggctg aagctttcac tttcaatatc
    18781 tcaacttcaa ggtataaggt atctcccggt gtaactttct tacggaattt tgctttgtcc
    18841 aatccgccga aataagccgt ctttcccttg aattgttcaa gagaaagtaa tgcaactgct
    18901 cctgcttgag ccattgcttc aacaatcaat acccctggca taacaggttc ttctggaaaa
    18961 tgtccttgaa aaaagggctc attgatcgtt acatttttct tagcaacgat ccgttctcct
    19021 tcaaccattt cttccactcg atcaatcaaa agcattggat aacgatgtgg aatgatttct
    19081 tttatttctt gtatgttcat tgttttccct ctttcctgct ttgcttattt ttgctgaatc
    19141 ctaaagagtg gctgtccata ttcgacaact gactcgtttt ctacaaggat ttctgtgatc
    19201 actccgtcgg cctcactagt tatctcattc atcagtttca tcgcttcaat aatgcagata
    19261 acttctcctt tggcaacttg atcacctact tttttgaaag ctggtttgtc gggcgctggt
    19321 tgcagataaa cgattcccac gattggcgaa acaacctcga ttccatcaaa tactgccgat
    19381 tctttttctt tcattatttc tggagcagtt gttgcttgtt caggtatgac attgactggc
    19441 tccgaaactg gcaacgtttc gtttgtttga accatattca tgcctcgact tgttttattt
    19501 ttattcatat acaattcaaa ttgaccttca cgcaaatcga attccgttag cgaggattga
    19561 tcaaattgac tgactaattc tctgatttca ttgatatcca aaattattcc tcccagcgtt
    19621 tcatgcacag aaccgcatta tgtccgccaa accctaatga attactcatt gcataagtaa
    19681 aacttgcagg tttgctttcg tttttgacaa ctgtcaagtc aatctcagga tcttgttttt
    19741 ctacattgat cgtaggtggg ataaattgat gttctaatgc cagcaaagtt gccaaggctt
    19801 cgatcccgcc ggctgctccc aataaatgtc cagtcatcga tttcgtgctg ctgacgtaag
    19861 tatctctgaa attttcacct aatgcgattt ggattgcttt tgcttctgcc tggtcattgg
    19921 ctggtgtact tgttccgtga gcattgatat atccgacttg ttccggctgt acaccagctt
    19981 cttccatcgc aagcttcatt gctttagatg caccagaacc atctggagta ggagctgtca
    20041 tatggtaagc gtcacagttt gcaccgtatc caacgatttc tccatagatt ttggcaccgc
    20101 gagcttgtgc atgttctaaa gattcaagaa ctagaacgcc tgctccttca cccatcacaa
    20161 aaccattccg attttcgtca aacggaatcg aaccacgatt aggatcagtt gattctgaga
    20221 gtgctgttaa agctgcaaat ccagcaatcc ccatctcaca aatcgttgct tctgttcctc
    20281 cagcaagaat gacatcagaa tagccatgtt tgatgttacg gaatgcttcc ccaattgaat
    20341 tcgttccgct agcacaagct gttacgatag ctgtacaaat accttttgca cctacacgca
    20401 aagcaatatt tccagctgcc atgttggcaa tcgccattgg tacgaacatt ggagaaactc
    20461 gttttgcacc tttttcatgt agacgaataa cttggttttc gattgttgga agaccaccga
    20521 tcccagatcc gatgatgacg ccaaaccggt cttgattcat tttttcttta tctaaaccgc
    20581 tcatctccat tgcttctaaa gcagcgtaga ttccatagat cgaaaacata tccattcgtt
    20641 tgctatcttt tttaataaaa tatttatcgt aagggaaatc tttgacttca cccgctaagg
    20701 taatccctgt ttctgaagca tcaaatttag tgatcggtgc gatgccattc gtcccctctt
    20761 ttaagctatt caaaaagctg tctgcgtcat ttccaatagg tgaagtgact ccatagccag
    20821 taattaccac tcgattcatt tgtttgctct ccttttctta tccgtgcatg accaatccgc
    20881 catcgacatt gatgacttgt ccggtgatat atggattttg tgctaagaaa actgctgttt
    20941 gtgcaatatc ttctacttgt ccgaaacgct gcatcggaat ttgcttttcc gcttgttcct
    21001 tgactttgtc tgctaaaact tccgtcatat cagttgtgat gaatcctggc gcaatggcat
    21061 tacatgtaat gcctctagtt gcagcttctc gtgccacaga ttttgttaat cctactacgc
    21121 cagctttact agctgcataa tttgcttgac caatatttcc aatcaaccca gatacactag
    21181 agagattaat gatggcccct tctcgctgct tcatcatttt tttcaatacg tgctgcgtca
    21241 tattgaaggt tccaatcaaa ttgatatcca gacatttttt gaaatcttct gcatccattc
    21301 gcatcacaag tttgtcattt gtgattccgg cattgtttac taacacatgg atcgatccaa
    21361 gcttttcttc tgcttctttg atcatttgtc ccgctttctc ataatcagag atgtcaccgg
    21421 aaactcctac gcattttaca ccgaatgctt cgatttcttc tatcttttct tttggaatct
    21481 ctccgcgacc attcaaaatg atatttgctc cagcttttgc aaaagctaag gccatcgctt
    21541 tgccgattcc tcttgtacta cctgtgataa atacattttt tccttttaca tccataacgt
    21601 tcctccttat agtattgcta ttgtttcatc aaatgtctgc ttattctcaa cacgcagaac
    21661 tggtattgtt ttatcgattt ttttcataaa tccgcttaat acttttccgg ggccgacttc
    21721 gatgacttgt tcgatgccga ttgtttttaa cttatggata ctctcataga aacgaacagg
    21781 ttgcatgact tgctgttcaa gtagtcccgc gattgtttct ttcttcatta tttccgttgt
    21841 tgtgttgctt atgataggaa aagaaggttc agagaattga atctgactta ggtccttcgc
    21901 cagttttttt gctgccggtt ctaaaatagc tgtatgaaaa ggcccgctga catttagagg
    21961 gatcattctt ttgaaccctt tttcttttaa tagtaaaaca gcttcgtcta ctgctttttc
    22021 ttcgccacca atcacgatct gctgaggtgt attataattt gcaggagata cgatgccgta
    22081 cttgctagct tcatgacagc tttcctcgat cgtttcaatc ggcgcattca tcactgcaac
    22141 cattttcccg cttccagctg gggcagcttc tgtcatataa gcgcctcttt ttgctactaa
    22201 cgcaactgct tcagtgaagg atagcgcatt actagctact aatgccgaat attctcctaa
    22261 actcagtcct aaagctgcat ctgggatgat tccatgctcc ttcaataagc gataatacgc
    22321 gatgctgact gttagaatag ctggctgtgt atattgcgtc tgatccaaac gttcattttc
    22381 tgtaaagcaa agttcagcca tatcgtagcc gagaatctca cttgcttcat caaatgtctg
    22441 ctttacaacg gcttcttcat ataaatcttt tcccatacct tgatattgtg caccttggcc
    22501 actaaataag aatgctgttt tcatgttttt ctccaatata tccagaatat atgtttgatt
    22561 cttcctattc aaaaaggccg agacattcct atttcaggaa atgtcccagc ccccaaccta
    22621 attttaatga ttatgcagtt tgtttttcta cgtaagtcac taaatcttga accgttttga
    22681 ttccgtcttc tgtttcgatt ttcacgtcaa attcatcttc aatttcattg atgatttgga
    22741 ataaatccaa gctgtcagct tctaagtctt cttggatgtc agtagtcaat ttgatttctt
    22801 cagtttcttt tcctaattct tctgcgatga tttcttgaat tttttcgaat accataataa
    22861 tatattcctc cgatattgtt tgctttttat ttttataatt taacagtcta caactccatg
    22921 agaatacttc cccatgttaa acctccgcca tagcctgtaa atattacttt ctgctgtttt
    22981 cctaaggtga tctttccact agaaactgct tcatccaata agatagggat gcttgcagca
    23041 gatgtattcc catatttatc catattggtc aggaactttt cttgcggcat ctttactttt
    23101 cgggcaattt tctcaataat tctgaggttt gcctgatgca aaagcagata atcaactggt
    23161 ttgtctttca tgacttcttg gatattgttt gcaacgtctc gtacggcaaa atcaaaaata
    23221 tctctgccag tcatttgcaa ataatacgaa ccttctgaat cttctgaata gaacggattt
    23281 ttattttgct ggtagccagc ggtcaatgag ctacttctgg taccgtcagc tgctaatttt
    23341 tctttcagca attttttctg tggattcgct tctaagagaa tcccacctgc tccatcgcca
    23401 aacaacacag cagtcgtacg atcagaccaa tcgattagct tagatagtgt ttcaccacca
    23461 atcaccagtc ccttagttga gccgcagcgg atcatttttt cagccatgct taacgcataa
    23521 acaaatcctg agcacgcagc actgatatca aaagcgatag catgagttgc tcccaatttc
    23581 ccttgtactt gtgctgctac agatggtgtc gtgtaatcgg gcgacattgt tgctactaaa
    23641 ataaaatcca aatctttagg gtttagattt ttctgtttta tcaatttttc cgccactaaa
    23701 atgcataaat cagaagtctc ctgatttgtt acacatcttc gttcacgaat acccgtcctt
    23761 gacgaaatcc attcatcaga ggtatccatc atcttggaaa gatcattatt agtaataatc
    23821 ttttcaggaa ggacgctggc tgttgcagta atcattccaa attgttccat taaatatcct
    23881 cacttatagt cttgcaagaa gtcgtgcaag tttttcagtg catccagcaa ggcatgctgt
    23941 tcctccgtag ccattccgtc taagatgttc ttgaccattt cacgatgaaa atgctgatgg
    24001 acccgaaata gtaactttcc ttttttcgtt agtcccaact ttacaacgcg gcggtcatct
    24061 tcactgcgga tacgttcgac atagcctttt tttactaact tgttaatagc agtggtcaag
    24121 gtcccaacag taattgatag ttctttggct acttctgaag ttgttttttt cttgtacatc
    24181 ccgattgcct cgatcgtgtg catctctgtg attgacagat ctttgaattg agattttttc
    24241 agctcagact cttcgattgt caaaatatca ttgaagacac taacaagata atcatttact
    24301 gtttttaaat ctggttccat gttccaccgt ccactaatta gtttgattat caaatctttt
    24361 gatgttcaaa ctatatctta acttttttca aaatgcaacc ttaaaagatc atttttttac
    24421 tatatgagct ttttttaaga ttt
//
LOCUS       DARBEC010000039        24259 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.039, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000039 DARBEC010000000
VERSION     DARBEC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.039"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(54..788)
                     /locus_tag="SCL80_001437"
     CDS             complement(54..788)
                     /locus_tag="SCL80_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEG1107314.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1115..1525
                     /locus_tag="SCL80_001438"
     CDS             1115..1525
                     /locus_tag="SCL80_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEG1107315.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1518..2219
                     /locus_tag="SCL80_001439"
     CDS             1518..2219
                     /locus_tag="SCL80_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEG1107316.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2219..3805
                     /locus_tag="SCL80_001440"
     CDS             2219..3805
                     /locus_tag="SCL80_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107317.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3824..4045
                     /locus_tag="SCL80_001441"
     CDS             3824..4045
                     /locus_tag="SCL80_001441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEG1107318.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4042..4803
                     /locus_tag="SCL80_001442"
     CDS             4042..4803
                     /locus_tag="SCL80_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEG1107319.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5183..5692
                     /locus_tag="SCL80_001443"
     CDS             5183..5692
                     /locus_tag="SCL80_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEG1107320.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5767..6306)
                     /locus_tag="SCL80_001444"
     CDS             complement(5767..6306)
                     /locus_tag="SCL80_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEG1107321.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SCL80_001445"
     CDS             complement(6446..7057)
                     /gene="rpsD"
                     /locus_tag="SCL80_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEG1107322.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7319..8170
                     /locus_tag="SCL80_001446"
     CDS             7319..8170
                     /locus_tag="SCL80_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEG1107323.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8198..8833
                     /locus_tag="SCL80_001447"
     CDS             8198..8833
                     /locus_tag="SCL80_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEG1107324.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8853..9626
                     /locus_tag="SCL80_001448"
     CDS             8853..9626
                     /locus_tag="SCL80_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1107325.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10034..10831
                     /locus_tag="SCL80_001449"
     CDS             10034..10831
                     /locus_tag="SCL80_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEG1107326.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10809..11222
                     /locus_tag="SCL80_001450"
     CDS             10809..11222
                     /locus_tag="SCL80_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEG1107327.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11206..13851
                     /gene="mutS"
                     /locus_tag="SCL80_001451"
     CDS             11206..13851
                     /gene="mutS"
                     /locus_tag="SCL80_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1107328.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13869..15977
                     /gene="mutL"
                     /locus_tag="SCL80_001452"
     CDS             13869..15977
                     /gene="mutL"
                     /locus_tag="SCL80_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEG1107329.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRETGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15999..16559
                     /gene="maf"
                     /locus_tag="SCL80_001453"
     CDS             15999..16559
                     /gene="maf"
                     /locus_tag="SCL80_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEG1107330.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SCL80_001454"
     CDS             complement(16644..18632)
                     /gene="treP"
                     /locus_tag="SCL80_001454"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEG1107331.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18855..19568
                     /gene="treR"
                     /locus_tag="SCL80_001455"
     CDS             18855..19568
                     /gene="treR"
                     /locus_tag="SCL80_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEG1107332.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19645..20091
                     /gene="msrB"
                     /locus_tag="SCL80_001456"
     CDS             19645..20091
                     /gene="msrB"
                     /locus_tag="SCL80_001456"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEG1107333.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20261..20863
                     /gene="ruvA"
                     /locus_tag="SCL80_001457"
     CDS             20261..20863
                     /gene="ruvA"
                     /locus_tag="SCL80_001457"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEG1107334.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAIEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20876..21877
                     /gene="ruvB"
                     /locus_tag="SCL80_001458"
     CDS             20876..21877
                     /gene="ruvB"
                     /locus_tag="SCL80_001458"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEG1107335.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21906..22484
                     /locus_tag="SCL80_001459"
     CDS             21906..22484
                     /locus_tag="SCL80_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1107336.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22536..23018
                     /locus_tag="SCL80_001460"
     CDS             22536..23018
                     /locus_tag="SCL80_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEG1107337.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23135..23509
                     /locus_tag="SCL80_001461"
     CDS             23135..23509
                     /locus_tag="SCL80_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107338.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
     gene            23939..>24259
                     /locus_tag="SCL80_001462"
     CDS             23939..>24259
                     /locus_tag="SCL80_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1107339.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHL"
BASE COUNT         7951 a         4359 c         5182 g         6767 t
ORIGIN      
        1 taaaaaaact tggtacgcaa tcgctgtacc aagttttttt atttagcttt tgattaagcc
       61 ataaataata atttatataa gaatactgct acgacaccag cacagatagg tgctacaact
      121 ggtacccatg catatcccca ttgagaagaa cctttatgtt tcaatggaag aagtgcgtgt
      181 acaagacgtg gagcaaagtc gcgggctggg tttagtccag gacctgtagg accaccaagt
      241 gaagcaacta atgtccatac aaggaaacca agtgctaagt gagctgtacc aagattatct
      301 tggaagaatg gtgcttttgt aatacctagg gcaccaaaga ataaaacgaa tgaaccaaag
      361 aattcattga cgaaaccatt gaatttagaa ccaacgctgt tgattgttga gaatgtacct
      421 aacacatggt ttacgtcttc tgtttggtcg tagtaaggtt tgtaacaagc tactacgatc
      481 aattgtccaa tcgctgcacc aatcaattgt gcaatgatat aaggaacaac ttcagcccaa
      541 gggaatagac cattagatgc taaagcaatt gtaaatgctg ggttgatgtg gttaccgctg
      601 attccaccaa aaatcattgc tggcatcata accccgaatc catacccgac agcaatcagc
      661 atccagtcac tgccatatcc ttttgtacct tttaaatcta cgtttgctac agcaccgtta
      721 cctagaacga ttagtaatgc tgtaccaatc atttccgccg caagacgggt cattagtgag
      781 taatccataa atacctccaa aagttataaa aatgcaaaaa aaaaagaaac atcaacacga
      841 aaagcatcgg tgcagtgttt cgtgtcggaa ggatttctgc agtcttaaca aatgtgaata
      901 aaaaaatgaa ttccattact tataagaaac cagaccaaca gtaaaactcc aactcttttt
      961 aaaagttctc cacaaatcac caagcttaac tataaatata ccaaaaaaaa aacgtttttt
     1021 ttctcaaaaa ttgtatctta gaaacagcat gttttgaaac tttcagaaaa ggtttgttag
     1081 taaaatgaaa gctatacgta aggagtgttg tttgatgaaa ttttttcaag agtttttcat
     1141 tattattttc ttttctgtat taggagaagg catacggatt tttgctcatt tacctattcc
     1201 tggaagtatc ttaggtattc tttttttatt tttagcattt gaaatgcatc ttgttgatcc
     1261 tgaaaaaatc ggagcaactg gtgatttttt attgaataat ctagccattt tatttgttcc
     1321 tgctggtgta ggtttattgg aatattttga tgatattgct tcgatatggc ctgttttact
     1381 gggagcagtc gtcgtgtgtt ccatcgtcac gatggttgca gcgggaaaaa cggcagaagg
     1441 tgtagaagct ttgctgggct atgtccgtaa gaaaaaagca gaaagggtat ctgtaaacga
     1501 aatggaggag aatcaaattg actgagtttt ttgtgaatcc attatttggc ttaagtttga
     1561 ctttaggagt gtacgtatta tctgacaaat ttttgcggaa atataaactg cctgttttga
     1621 atcctctcgt attatcgatt gttgttatta tcttattttt gcatttttct gggatcagct
     1681 ataaagacta taatatcggg ggcaattttt tgactatgct gattactcct gcgacagtgg
     1741 cacttgccat ccctctttat aaaaacttcc gtttactgaa agagaatttt tatcctgtaa
     1801 tggctgcgat tttagcagga atagtcgcaa attgcttggt tagtgtaggg ttcggttact
     1861 tcttctctct tcataaagag atggtgatct cattactacc taaatctgta acgacggcta
     1921 tttctgtcga tttatctcat acgatgggcg gggtgaatgc cgtgacactt gctattgttg
     1981 tctctacagg tatttttggt tcgctgatcg ccactcatat tttccgccta ttcaagatcg
     2041 aaagtccagt tgctcgcggt gtggcgttag gaagtacttc tcatgctatt ggtacagcaa
     2101 aagcaatcga gattggtgaa atagaaggaa tcatttccgg acttgcgata tgcgtcaacg
     2161 gaatattgac cgttttattg ctaccgttat tctttcagcc ttttgctggc ttgttttgat
     2221 gcgaaaacca ctattttatg ctacaataac caaaacgttc gagcataaaa aaggagggaa
     2281 agacgtgtat ttgtcttccg agtatcttaa acaatttgat aaagatctat attataagat
     2341 tctcctccta gaaagttttg aggatcaagc ttggcatacc gctgcacaat tagcgcaagt
     2401 tgttcagctg gatgcccgca gcgtgtcgaa atacctgaac gagctgtcta aaaactatca
     2461 gcagtttagc ggtaagacac accctttgtt tacaaaaaat caccgttctg gttataactt
     2521 ttatgatacg ctcgattcaa ttgaacatga gcgttttttg atctatctcg tgcaaagtac
     2581 gctgaaattc cagttgctgc acgacatttt ttttgaagaa ttccatacaa tgtaccaatt
     2641 tgcgcaaaag cattacatta gtgaatctac tgctcatcga aaaatcaatg aatggaaaca
     2701 gcaactgcaa acttacggaa ttcggctgca acgaggtaca tatatcgctc aaggcgaaga
     2761 agaaatcatt cgactttact tgcatatgac attctggcag ttgtttcgag ggaaaatctg
     2821 gccatttgaa acgatttctc aaatggacgt aaagaatatg gcagaacata tcatggcttt
     2881 ttttaatgtt cgtctgaacg agatcaaaaa aagacgattg gaatatatgc taggtgcctt
     2941 ttttttaaga aaatcacaga agcattatgt tgtattgaat gagaaaaaaa gacggctgat
     3001 ttctgacaat ctgttgttcc agcgattttg ccaggtcatg gagccggtat ttcctaatta
     3061 ttttcaagta gaagacgagt taggcgcatt atttcttgta ctgatgactc gtgaagaata
     3121 ttatagtgat cctaagatac ggaagaaaat ttttgatttt catcaacaag caaaaacacc
     3181 gccctttaca gccctttcag aagcaaaagc cgctctttct ctttatcagg aagagcaggg
     3241 gctgccagca gaaaatctaa ccttcgaggc agaaaactat ttattcagca gtcatttttt
     3301 cgcctatctt tttcctaatg cgaaagaaac gattgatggc aatagtagtg attttattaa
     3361 tcatttagtt attgaaaaca aggaattgaa acaatggctc gtacattttt ttgaaagtag
     3421 acacaaacat cccaatcatc tggcatttaa aaatcatgcg tttttgatgg ggcggtatct
     3481 gactgtgttt aaaacattag gagcctttac tccacagctt ccaaagatca cgatcctttt
     3541 aatgacagat ttcccattat ttgaagagca actgttagag gagggattac ggacattttt
     3601 ccgtaatgag tatcagctga tttttctgcc aacagattat cgggggagag aagttgatct
     3661 tctgatttcg acaagtaaag tccaccgcaa accatgggcg gatcttgact attttattgt
     3721 gactgaagaa ctaaaattaa ttgattacat ccaactatct caaaaatttg aaatgattca
     3781 aaaacaaaaa ctattaaaac aataacaaag aaggagaaaa aagatgacat ttgaagaaat
     3841 tttaccagaa ttgaaaaaag gtgcaaagat tattcgtaaa ggatggagtg gatttgaact
     3901 ttatgttgtt ctcgtatccg atgatgaatt tgatggctgt ccggtgacgc cgtacttatt
     3961 gatcaaaaca agtgacgaag gtttttccag cttcgctcct acagtttgtg atattttagc
     4021 agatgattgg gcgatcgtag aatgaaaaac cagtatccag atctagctaa acaggttgta
     4081 tttatcacag gagctgcttc tggaattggt gctgcgcaag cccgagcgtt tcttgaacaa
     4141 gatgcttttg tatttggatt agatcaaaag ttcggtaaaa tggaagaaat ttctttgcga
     4201 tttcctgaaa cttttgccta cgcactaggt gatgttcgac aaatgaaaga actccaacaa
     4261 gcggtatcga aatgccaaaa tcattttggt gaagttacga ttttactgaa tacagcgggg
     4321 atattggatg cctacaaacc cttgatggaa actgacgaat cgctgtggga tctgatttat
     4381 gagacgaatg tgaaaagcat gtatcagctg acaaaaatca tccttcctga tatgatccgc
     4441 caaaaaagcg gaacgatcat caatatggct tcgattgctg gcttgattgc tggtggaggt
     4501 gggatcgctt atacaagcgc gaaacatgcg attgtcggat ttaccaaaca actcgctctg
     4561 gattacgcag gacagggtat ccgagtaaaa ggaattgctc caggagccat ccaaacacca
     4621 atgaatgcgg ctgattttga agggaaaggt gagatggcag agtgggtggc aaatgaaaca
     4681 ccagtcaagc gatgggcaca gccggaagaa gtagctgagc tgacgttatt tcttgcctct
     4741 cctcaagcaa gctacataca aggaacgatc atcccaatcg acggcggatg gttgttaaaa
     4801 taaccttgtg tttttttcaa gcttctgtat gataaaacta aagagagaaa caacggttga
     4861 tgataaaaaa agtcgatgac tgttcccgac attcgatgcc gaacagccag ttcaaaaaat
     4921 tctttttaat aatcgttaat attcgaaact aagcggcttt tcacgacctc ttgatataaa
     4981 cgatattcaa aagcttgcaa ctgcggtaac aagtccagcc aaatgaaaaa tataaggagc
     5041 tatttttatt tgaccattct tattacttgt tgcagggcag ctttttcact accttctgcg
     5101 acacttcagt tggaattttc ttcccatcct ttggtcgcgg tcattaccaa gataaaatta
     5161 tcttgcaaag gagaaataga acatggaaaa aaataatacc cgttcagcca aacgctggac
     5221 aacgacagat gtgactcgga tggcgctagt cactggttta tatgtggcag tgacgctggt
     5281 tttgtctgtc tttagctttg gagtgattca gattcgtctc tcggagatgt tcaactatct
     5341 gtccttgtat aacaaaagat acatcgtcgc ggtaacagcg ggagtagctt tggctaatat
     5401 tgcttctcct cttggactga tagatgttgc agtaggaagc gtcagcacgc tgcttgtatt
     5461 gcttatcaac tataaaatca ctagtcgcat caaaaacatg aaaatcaaaa tggccgtgac
     5521 agcatgcgta tttgcttttt ccatgtttac agtagctggt cagctaacga ttttatatca
     5581 actacctttt ttcttgaatt ggtgggtgat tgctttagga gaattggcat caatgattgt
     5641 cggtgggata attatttatt ggatcggtaa aaaagtggat ttgtcaaaat aaagatctgt
     5701 ttaatttaaa atagggcagc atggaagaga ctgaatgttg agtcttcttc catgctgctt
     5761 ttttgattaa tttgtatgaa gaaatcgatt tagccgtttt ttcatcaaga taccgatata
     5821 agctgctcca gttgccttga ttgcttccgg cagtaaaaac ggcagtagtc cactggcaaa
     5881 tgcagttgta aaagtaagat caccacttat tttcagccag atcgttccgc agatcaatgc
     5941 agcaagagag cccattagat tggcaaagat tgcatataag taatgagcat ccgttctttc
     6001 gatcatccaa ccagttatcc acgtttgtaa gagaaatcca atcaaatatc cgccagttgg
     6061 cccaaataaa acagctatac ctccagtcat ccctgaaaat acaggaagac caatcaagcc
     6121 aagtaaaaga taaactccaa cagatatcaa tcctagtttt cttccgagaa aagttgctgt
     6181 taatccaata gcaaatgttt gccctgtcaa aggaaccaaa ccaaaaggaa tggtcatctg
     6241 agaaaggaca gaaataatcg cggcaaattc cgcagcaagt atcaattctt tcaatgttcg
     6301 gttcataacg ccctcttttc gttaacttaa tatttttatt tggttaacga aattgtaaaa
     6361 caaagtgatt actttgtaaa tagaaaatat aaaaaaagac cagctatcca atagatagcg
     6421 gtctttttca agaaagcttt tgaaattata agctttggtt gtagtattca acgacaagag
     6481 cttcgtcgat ttctggatat agttcgtcac gttctggtaa acgagtcaat gaaccttcca
     6541 atttttcagc gtcaaagctt acgaatgctg ggcgtccaac agttgcttct acagcttctt
     6601 ttactgttac gatgttttgt gatttttcac gaactgagat cacttgaccc acttctacgt
     6661 ggtatgaagg gatatcaacg cgtttgccat caactaaaat gtgaccatgg ttaactaatt
     6721 gacgtgcttg acgacgagtc gttgcaagtc ctaaacggta aacaacattg tctaaacgtt
     6781 gttctagtaa gatcatgaag ttaacaccat gtttaccttc tttgattttg ctagctttta
     6841 cgaacaagtt acggaattga cgttcgttca ttccgtacat gtgacgaagt ttttgttttt
     6901 cagttaattg catgccgtat tctgattgtt tgccacggct attaggtcca tgttgacctg
     6961 gtgcgtaagg gcgacgtgct aattcttttc ctgtgcctga caatgagatg cctaagcgac
     7021 gagaaatttt ccatgatggt cctgtataac gagacattaa aatttcctcc aatgaatata
     7081 agtttttgga gtaaaataat ccgttgaaaa attcataatc atatagtttg ttcttcaatc
     7141 ttcacctttg cagccgcggt tacacaatcg aacccgagac ggggcaacaa actagggatg
     7201 agttattatt tttctgctgc attattttac acaaattaca gtatatgata gatcatccac
     7261 gaatgtcaag ataattagta cataaaaacg ctttacaaac ctaggctgtc tttataaaat
     7321 gagagggtat gtgcaaatgg aaaaaagcga aggaagttta aggagaaaat caatgaatcc
     7381 ggtatcacca ttatttgaga aaatcgatgc ttcaattgaa aagaaggcga atctttttga
     7441 aaacagcttt tcacgttacg ctgttcgagc ggttctcgcc tgtttgtttt tgactttagg
     7501 aacggctgtg gcatttgcga tcgcgatgaa gggggaagat atcgcgcatg gtctaggaaa
     7561 gattttatac gcatttatgt tcagctgggg tctggtcatg attctataca tgaacgccga
     7621 attaggaaca tcgaatatgc tttatatgac cgtgggtgtt tttcggcaga gacttgcttt
     7681 aagtaaggca gccaagattc tatttgcctg tatttttttc aatatgattg gcggtatatt
     7741 atgtggattt ctaatttcta tgactacgcc atttcaagat ttaacgttgg acaactttat
     7801 gttagaatca atagcagtaa aactagcaaa accttcagca acgattttgg tggaagggat
     7861 gtttgcgaat atcgttgtca ataccgctgt actgatcagc atgcggatga aagaggatgt
     7921 cggaaaagtg atcacagtcg tattcatcat tttcattttt gcttttttag gatatgaaca
     7981 cgtgatagcg aattttcctg cctttacgct tacttacttt gcttctcacg gacagatgga
     8041 tggaatgaca gtcagcaatg tgttacataa tttgtttttt gctttggcag gaaattatat
     8101 tggtggcggt ttagtaatgg gactgggata tgcttggctg aatcagtcaa aatcatccta
     8161 tgtggactaa aaagtcgttc attaaagggg aagtgccatg gaagttttag tttttccaaa
     8221 aaatgaggaa aaagcgttac aaatggaaaa gtacatgaaa aatctttttt cctttgctgg
     8281 agtacctaag ccggaacgcg ctcttttaca acaaccgtta ctggatgaga gcaaaaattt
     8341 atctatttct gaagttttag cttttgttca ttattattat gaacaaatcg aaagagagta
     8401 tcagtatgta gcgatcgatt tagtccagaa aaacatcaaa cggcttacat ttgaagactt
     8461 agtcgtattg ttgccactag ttcagaaaaa agaatggtgg gacagcatcg actcttggag
     8521 aaaagtatac agcacatggt gtaaagccta cccagagcag cgtgaagaag tctttcaatt
     8581 gtttgaaaaa aaggaagatt tttggctacg tcgaatagca atcacgttgc aattaggtta
     8641 taaacagcaa acagacacag gactattgat ccgagcaata gacgaagatc gtcaaacaaa
     8701 ggaattcttt atccaaaaag caattggttg ggcattacgc gattatagta agaccgatgc
     8761 cgattgggta aaaagacagc tgacaacaga tttgagtaag ttagctgttc gagaggcaag
     8821 taagtattta taagaaagcg agacgacaag agatgagacg caaattattt gcttttgata
     8881 ttgatggaac gttgttagat tcaaacaaac aagctttaga tagcacaaga gaagcattgg
     8941 aaaaattacg caaagccggt catctggtca cgattgctac agggcggagc cgatttcatg
     9001 cgcaagagat cattcgcgat cttgatttta cgaactacat tttatgtaat ggagcggccg
     9061 gttttttaga ccatgaacaa gtatataaga atttactaga tcgcgaccag ttggatgcgt
     9121 tcgttcaaga agcgcatcat gagcagatcg atacagcttt tgtcagctta gacaatatca
     9181 agcgttcttc ttccaatgat gtaacggtga tggaagaagc gatgcattct ttcggtgctg
     9241 tcttgcctga attagatcag ttttttgcag aggaacaaga aatataccaa gcgttagcat
     9301 ttttcgatca aacatatgat gggaagttct cttcatatga taaaattcga tttgtccgct
     9361 ggcatgagaa cagtgtagat gtcgtaccta aaaatggttc taaagcagcg acaattctgc
     9421 acttagcaga acaagtaggc atccctcatg aagacatcat cagctttggc gatggacaaa
     9481 acgaccgtga aatgttgcgc atgtcaggaa ttgggatagc tatggggaat gctgtgccag
     9541 aagtacaagc agaagctaag atggtcactg atacaaacga tcaggatgga atttggaagg
     9601 cattgaaaga gttaagcgtg atttaaaaga ggttgtgaaa aagctgttct gcatcaagta
     9661 ataagcccag ccaaacaaaa atagctcctc ctatttttcg tttttctggg cttattaccg
     9721 cagatgcaag ctttcgaaca ccgtttaagg aagaggtcgt gacagaagtg gtcagtttca
     9781 agaaataaga agggatttac gaaaattgct tctcaaattt ttgtaaaatt acggcttatt
     9841 tctgaagaag tagatgaaac aacgtttatt cgggaaatgg gactgtgaca agatgtttgt
     9901 catagtccca tttttttatt ttatttttca aaactaagaa ataccaggaa agagttcctt
     9961 ttgtttgacc ttttctttct tattgaaaac aactataata tagagtgatg ttaaaagaga
    10021 agaggaattt agtgtgcggg tattgtttat tggtgatgta gtaggttcaa tgggacgaga
    10081 gatgattact gaatatcttc ctagattgaa gaagaagtac cgtccgcaag taacgattgt
    10141 aaatggtgag aatgccgctt ctggacgtgg aatcacagaa aaaatctata aaaaattttt
    10201 gcaagacggc gtagatgtcg tgacgatggg gaatcatacg tgggacaacc gcggaatctt
    10261 tgagtttgta aatgaagcta aaaagatggt tcgtccggca aattttccgg aagggacgcc
    10321 tggacaaggg atggtctttg tcaaagtgaa ccaagtcgaa ctggcagtga tcaacatgca
    10381 ggctcgttct tttatggtgg acttggatga tccttttcgt aaaatgaatg agctggtaga
    10441 agaagccaga aaacgtacgc cgatcatttt tgtagatttt catggagaaa caacaagtga
    10501 aaaacaagca atgggctggt ttttagatgg gaaagtttca gcagtggtcg gtacacatac
    10561 acacgttcag acaaatgatg cgcgaatttt gccaagagga acagcgtatc tgacagacgt
    10621 tgggatgaca ggtccctatg atggtatttt gggtatgcgc agagaacctg taatcgaaaa
    10681 gtttctgact gcattaccaa aacgttttga agttgtggaa caaggccgat cgattttaag
    10741 cggctgtatc ttggaattgg atgatacgac aggtcatgca aaagaaatcc agctgattca
    10801 gatcaacgat gatcgaccgt tcatggagta gaaagagagg cagttttttg cagcaggagc
    10861 cggaaattca aaaagaagta aggaaactga caaaactttt aagagagaat gaaacgatca
    10921 ttcgctacaa agaacttgaa gaaaagatcc agcaaaacca gtatttagct gagttgagag
    10981 aaaaaatcaa acaagcgcaa aaagatgctg ttcattttgc ccactatgac aaaccagcag
    11041 cagaaaaaga agcaatcaaa caagcggatc aattcatgca agaatttgat cagcatccgc
    11101 ttgttgtagc ttatagaaaa cagctgttgg aagcagatga tctgcttcat catttaacaa
    11161 ctatgataca agaagaaatc aatggacaaa tagaggagga aaaacatgcc tcaaaaaact
    11221 aaaaatactc ctatgatgga acaatatcta agcatcaagg cccaatacca agatgctttt
    11281 ttgttctatc gtttaggaga cttttatgaa ctattttacg aagatgcgat caaagcgtca
    11341 cagcttttgg aattaacgtt gacgagcaga aatcgaaatg cagaagaacc tattcctatg
    11401 tgcggtgtac ctcaccatgc agcacaagga tatatcgatg ccttgattga aaaaggctat
    11461 aaagtagcga tttgcgagca ggtagaagat ccaaagacga ctaaagggat ggtcaagcgc
    11521 gaagtgatcc agctagtcac tcctggtaca gtcatggaca gcaaaggatt gtcggcaaaa
    11581 gacaacaatt ttttaacagc tgtagttagt caaggaaatg aatttggctt tgcttatgcc
    11641 gatttaagta cgggagaatt gaagacggct cggttgcatg atgaagaagc tgttttaaat
    11701 gaagcctctg cattgcagac aaaagaactc gtattaggca gtgagatcac tgataccctt
    11761 cgtgaacagc taagcgggcg tttagggcta gtcttttctc aacaaaatga gatggaagaa
    11821 aacgcggaat ttagcttttt gacaagcgaa ctagtcgatc cgctggaaaa agaagcaacc
    11881 ggcaaactat tgacttattt atcggtgaca caaaagcgga gtttagctca tatccaaaaa
    11941 gcagtagaat accaaccaga tcattttttg aaaatggatt attattcaaa attcaaccta
    12001 gaattaagcc aatctattcg gactgggaaa aagcacggca ctcttctatg gttgttagat
    12061 gaaacaaaaa cagcaatggg tggccgttta ctaaaacaat ggctagaccg tccgcttatc
    12121 caggcaaaac aaattaaagc aagacaagaa atggtcgctt ctttgctaga cagctatttt
    12181 gaacgtatcg atttgcagtc agcattaacg aaagtctatg atctggaacg cctagcaggt
    12241 cgagtagctt tcggtaatgt caatgggcga gacttgatcc agctgaaaac ttctttagaa
    12301 caagtgccaa tgatcagagg gttgattgaa ggaatcgatc aaggtcaatg gcaaagtctg
    12361 ctttcagaaa tgcagccgat ggaccatctc gtccaattga tcgaccaagc tatccaagat
    12421 gatccgccgt tacaaataac ggaaggaaac atcatcaaag atggattcaa tgagcagctc
    12481 gatatgtacc gttctgctat gagaaacgga aagaaatggt tggcagaact tgaagcaaaa
    12541 gagcgtcaag aaacaggaat caagaactta aaaatcggat ttaaccgcgt tttcggttat
    12601 tatatagaaa ttacaaaagc aaatcttgga aatgcagaac tggagaagta tgaacgaaaa
    12661 caaactttag caaatgcaga gcgtttcatc acaccagaac taaaagaatt ggagacacaa
    12721 atcctcgaag cagaagaaaa atctgttgat ttagaatatc agttgttttt agctgtgaga
    12781 gaagaagtca aaaaggctat ccagccgcta caagtacttg caaaagcaat cagtgcagta
    12841 gatgtgctac aaagttttgc aacgatcagc gaacgctatc agtatgttcg gccagaactt
    12901 gtcagtgaca agcatcagct tctgatcaaa gacggacgac atcctgtagt agaaaaagta
    12961 ttaggacatc aggaatatat ccctaacagc gtagaaatgg cagaagacga aatgatcttg
    13021 ctgattacag gaccgaatat gtctggtaaa agtacgtata tgcgacagtt ggcattaact
    13081 gttctgatgg cgcaaatggg ctgtttcgtt ccggcaaaac aagcagtttt acctattttt
    13141 gatcggattt ttacccgaat tggtgcaagt gacgacttaa ttgctggaca aagtactttt
    13201 atggtagaga tgatggaagc caatcaagcc ctgcgttatg ctacgccaaa cagtttgatt
    13261 ttgtttgacg aactagggcg aggaacagca acctatgacg gaatggcgtt ggcccaagca
    13321 atcatcgaat atatccatcg tcatgtacaa gcaaaaacac tcttttctac tcattatcat
    13381 gaactgacgg tacttgagaa agaacttccg cagcttaaaa atgtccatgt tggagccgtt
    13441 gaaaaagacg gagaagttgt cttcttgcat aaaatgatgg atggacctgc ggataaaagc
    13501 tacggcatac atgtggcaaa gatcgcagga ttgccttcca atctactaga acgagcagca
    13561 gatatcctgc atacgctaga aaatggcgat aacggacacc atactgttcc agctgaagta
    13621 gaagagaaaa ctaaaacagc caaacaggag caaataaaac cacctgcaga aaaagtaaat
    13681 caagaccata tacaagaccg tgtaaaagaa gaaaaccaac agctttcttt attcagtgaa
    13741 ttatccgaaa atgaaacaga agtcattgaa tcactgaagc aaataaattt actagaaatg
    13801 acgccaatgg atgcactaaa cacattatat gaattacaaa aacaactgta ggaagaaggc
    13861 ggatatcgat ggccaaaatc caagaattat ctgaacgttt agctaatcag atcgctgcgg
    13921 gagaagtagt ggagcgacca gcttccgttg tgaaagaatt agtggaaaat gcaattgacg
    13981 ccggcagtac tcagattgat atcttagttg aagaagcggg actgcggacg atccaagtga
    14041 ttgataacgg agaaggcatt ttggcagacg acgtagagaa tgccttcaag cggcatgcga
    14101 caagtaagat ccataatcgc gacgatcttt ttcgcatacg aacgctgggc tttcgagggg
    14161 aagctttgcc aagtattgcc tctgtttctg aaatgactct ggaaacggcg aatcaggaag
    14221 aaaaacaagg cacctatctg tcgctgaaag gcggagaagt tattgagcat cgaccagcac
    14281 cactgcgtca aggaacgaag attaccgtca gcaatctgtt tttcaatacg ccagcccgct
    14341 tgaaatacgt gaaaacactg cacacagaat tagcgaacat cggggacatc gtcaatcgac
    14401 tggcattgag tcatccatcg attgcttttc gtttagtaca tgatggaaac aaaatgctgg
    14461 caactgctgg aaatggcgat ctgaaacaaa caattgcggg aatctacgga ttatctaccg
    14521 caaaaaaaat gctgaaaatc gaaggaaaag atctagattt tgagttattt ggctatgttt
    14581 cattgcctga ggtgactcgt gccagtagga attacttatc gacgatcatc aatggacgtt
    14641 ttatcaagaa cttttccttg aataaagcaa tcgttgcagg ttatgggtca aaattaatgg
    14701 tcggtcgttt ccctattgct gtattggaaa tcaaaatgga cccgctttta gtagatgtaa
    14761 atgttcatcc gacgaagcag gaagtccggc tgtcgaagga aaaagaacta acagagctaa
    14821 ttagtaacgc cattcaaaaa gcattgtctc aagaaaatct gattccaagt gcagcggata
    14881 atctacggtt taagaaaaaa gtacctgatc agcctaaaac agaacaaatc gaaatcgatt
    14941 tgtcctacgc ttttgaagaa gaaaaaccta aaaagccaag tagtttgaat tttgatcgag
    15001 agacaggtac ctttttcgta gaagaactaa agaacacatt tgttgacaga attgttgata
    15061 agtctgtgga aaatgttgaa aactattctg gtttttctga agaaaacacc acttatccag
    15121 aaaaaacagc gaaaacggtg gataagtttg gtgaaaatgt tgataacttt gtagaaaaaa
    15181 cagtggaaaa atctgaaaag aaagaatttc ccattgtgga taacatggag aaaacaaatg
    15241 aagaacaagc agataaatcc gtagaagagg atgaaaagat ttacgaggaa gaattgacac
    15301 tccatcctga atttgatttc actcaaggaa acgacaaaac attagaacaa gccatcaaga
    15361 aattagaaac ggaaaaacca gtacaacgtt ttcctcagtt ggaatatttt ggacagatgc
    15421 acggaacgta tctttttgcc caaagtagcg atgggttgta catcattgat cagcatgctg
    15481 cccaagagcg cattaagtat gaatatttcc gggaaaaaat tggggaagta tctgatgatc
    15541 tgcaagaatt gcttgttcca tttgtgttag actatccaaa cagtgatgct ttgaaattaa
    15601 aagagcaaaa acacttgcta gaagatgcag ggatttattt agaagaattt ggtcaaaaca
    15661 gctttattgt acgggcccat cctacttggt atcccaatgg agaagaagaa tcgattattc
    15721 gggagatgat cgatatgctg ctgacaactg gaagtgtgag tgtaaaaaaa ttccgtgaag
    15781 caacagcaat catgatgagt tgcaaacgat cgattaaagc caaccattat ttgaatgaag
    15841 cacaagcacg tgtgctgctg aaggatctgg cacgatgcga aaatcctttc aattgtcccc
    15901 atggccgtcc agtattgatt cactttacca attcggatat ggaaaggatg ttcaaacgaa
    15961 tccaagaccc gcattaagtc aaagagaaga gggaaaacat gaagattgta ttagcatccc
    16021 aatcaccaag aagaaaagag ttattggctc atatcgtttc agaattcgag attgaacctg
    16081 ctgatatcga tgaaacgcca tttccagacg aacagcccgt cgattatgtt cgtagaatgg
    16141 ctgaagaaaa agcacgaact gtctgggaac aagatgaaaa agaagaccat ttagtgattg
    16201 ccagtgacac gactgttgtt ctcaatcaag agatcatggg taaaccagaa gatctggctg
    16261 aggctgaatt catgctgaaa aaactaagcg gggaaacaca tactgtatat acagctgttg
    16321 ttttaaaaac aaaggaacga gaagaacgga tactagcaga agcacatgtc actttttatc
    16381 ctttgacaga cgaagagatc aaacgctact tggagacggg ggattatgcg gataaggcag
    16441 gggcatacgg catccaaaat gctgcgagtg tattcgtcaa agaaatttct ggagattatt
    16501 acagtatcgt aggatttccg attggtgctg tcaatcaggc actgaaaaca ttcatttaaa
    16561 caaaaaagcg gactatctga ttttcgattg aaaatccatg atagcccgtt ttttttattg
    16621 aaaagattac ttatctactc tgactaagat tttacttcag ctttaccgac tgcttcgccc
    16681 gggcgaacaa gtcctgattg gtcaagagaa agtcctgcaa ctttttcatt attggtcaaa
    16741 gcaacaataa tgtcagtttt cttgccagct tgttctattt ctggcaaatc catttcagca
    16801 atcagggtct ctggtgtgac aaggtatcct tctttcacga atacattgaa aggaacaccc
    16861 tttagttcaa cagtatctaa tcccatgtga acaagtactt ccagcccctc attagaaaga
    16921 ataccaatgg catgttttgt ttcaaagaca ctcgtcactt taccgtttac tggtgcatag
    16981 actttgccgt ttgacggttc gacagcataa ccgtcaccca tcattttttg tgagaaaact
    17041 gggtcattta cttcagttat ttccttgatg gtccctgcag cgactgcgaa taatgtttct
    17101 tttgttgcaa ctgccgtacc agttgctgaa acaggttcga aagactgtgg cgttgctgtt
    17161 gcgccatttg ctgtttgaag ttgtacatca gcagcatttt caggaacagc aggatcaatc
    17221 ttattcaaaa ttccctgacg tctaaagatg atcgtcaagc caaatggaat gatgacagca
    17281 atgatcattg cgatgatgaa aggtacccat gtttctgctt ggatagctag tattcctggc
    17341 aaaccgccta ctccgatcgc gttagctcga acacccatca agttagcaaa catgccagct
    17401 aatccagagc cgaccattgc agcgacgaaa ggatagacgt atttcaaatt gattccgaac
    17461 atagcaggtt cagttacgcc taagtaacaa gaaatcattg ctggaatcga gatttgttct
    17521 tcttttttat tcccacgatg taagaagatg atggctaaga ctgcagagcc ttgggcgata
    17581 tttgataaag caatcatcgg ccataaattc gttgatccga aatctgcaat cagctgcata
    17641 tcgatggcat tggtcatatg gtggagacca gtaatcacta aaggagcata caggaatccg
    17701 aatacggcac taaacaacca gctgatagaa gacgtcaatc cagtatttac gatatttgag
    17761 atccagcttc cgactgtcca gccgatagga cctaaaataa cgtgtgcagc taaaacagtt
    17821 ggtaacaatg aaaacaatgg aacaaaaatc atcgagatcg actgagggat atatttgcgg
    17881 aagaagattt ctagatacgc taacataaac cctgccagca tggctggaat aacttgcgct
    17941 tgataaccaa tcatctggac ttgagcaaaa ccaaaatccc aaaatggaat atctgctgcg
    18001 gctgtggaag ctacactata agcatttaat aattgcggag aaactaacgt aattcctaaa
    18061 acgattccta atatttgtgt agtacccatc ttttttgcaa tactccaagt gatacctact
    18121 ggaagaaaat ggaaaattgc ttcacccggt aaccacaaaa aggcattgac accattccaa
    18181 aacttcgagt gttcaacgat tgttccacca agactttcaa actggatacc ttcaagtaca
    18241 tttctgaatc caaggatcaa tccaccgaca atgattgcag gaatcagtgg tgtaaagatt
    18301 tctgctaaaa cggcaatagc acgttgaacg ggatgaaggt tttgttttgc agctgctttt
    18361 ccttgttctt tggaaacccc ttcaactcca gatactgcaa cgaagtcgtt ataaaaagta
    18421 gaaacatcgt ttcctatgat gacttggaac tgtcctgcat tcgtgaacat cccttttacg
    18481 ctaggtatat cttcgatcgc tttttcgtcc gcttttcctg gatcattcag gacaaaacgc
    18541 atacgagtcg cacaatgact aactgcagca atattttctt ttccgccgat atcatgtaac
    18601 agcttctcgg catctgcttg atattttgcc atatcaataa acccctttct cttaacttgt
    18661 ctatgcaagt tgtttcttca tttactatac tttttttcgg agagaattgc aagcgtttta
    18721 ttaaaaagta cgagagaaaa tgatatcgtt ttaaaaagct taaaataagc ttttcttaaa
    18781 aataggaaca ttgaaataca aaaagagctg tagtatagtg aagaaatgat ggaagaacga
    18841 gtgagggaaa aaacatgaat aagttcaatg aaattttttt agatttggaa cagaaaattt
    18901 tagcgaaaga atatcctccg cattcgcttt tacccagtga gaaccaattg atcaaaattt
    18961 atggtgtttc acgagaaaca attagaaaag cgctgaatct tttgactacg gaaggttata
    19021 ttcaaaaaaa acaaggaaaa ggttcgatcg tactggatcg gaatcgcttc gactttccta
    19081 tttctggatt gactagctat aaagagctac aggagaccca acgtattcca agtgaaacga
    19141 tcatccatac gttagaagaa acagaagtga caaaagcatt gaacgagatc actggctggg
    19201 agatcggtgc gcctgtttgg catttgatac gcgaaagaaa aatcaatggc gaagtagtta
    19261 tattagacac cgattatcta ttaaaggaaa tcgttcctat tttaactcca gcacaagcac
    19321 aaggatcgat ctatgaatat tttgaaaatg agctttcttt gacgattgct tacgcgcaaa
    19381 aggagatcac agtagaagaa gtaacggaaa tcatgaaaac agcgatggat cttcacgatg
    19441 acacttatgc agtagtagtc agaagcctcg ttcatctgga agatacccgc tgtttcgagt
    19501 attctgagtc ggttcaccga ttagataaat tccgtttcgt tgagtttgcc cgacgccgaa
    19561 aagtgtaaat agttcaaaag aaagcaaatt gttggcatct gtccactttg gcgctatgat
    19621 aggatcacaa agggggcatt tttaatggat aaaccaataa gaccagaaaa agaagaactg
    19681 aaagaaaagt tatcacccat ggcttatgca gtcacacaag aaaatgcaac agaacgacca
    19741 tttagcggga aatatgatga tttttacgaa aaggggatct atgtagatat cgtcagcggt
    19801 gaaccgttat tcagctctgc agaaaaatat gatgcaggtt gtggctggcc atcattcagc
    19861 aaaccaataa cgaaaagaca agtgcgcgaa aaagccgatt tttctcatgg catgcatcga
    19921 atagaagttc gaagcaaaga agcagattct catctcggac atgtgtttac cgatggacca
    19981 gtagatcaag gtggtctacg ttactgcatc aattctgcct cactgaaatt cattccatat
    20041 gaaaagatgg atgaattagg ttatggggaa ttcaaatcac ttgtagaata atggaaatac
    20101 acaaagagcg gtacaatacg gggatttact cgatcgtccg ctcttttttt attcaagcga
    20161 ttgctatttg aaagaaacgc tccctagact ccttttttca caaggtgaaa tgtgctacaa
    20221 taaagggaac gtatgtgtgc aaaagaaaga ggactagaat atgtatgaat atctgacagg
    20281 tgtggttacc tttatcaacc cctattacat agtagtagaa accaatggta tcggctacca
    20341 gatcgcacta ggaaatcctt accgctacag tagcaaacta aatcaaaaaa taaaaattta
    20401 tgtccatcag gtaatacgtg aagatgcgca tcttctgtat ggatttgatt cgctagaaga
    20461 aaagcaactg tttttgcgat tagtaagtgt ttcaggaatc ggtccgaaaa gtgcccttgc
    20521 aatcatggca agcgaagacc actccggttt gattcaagca gtagaatcca gtgatgtcac
    20581 ttatttgacg aaatttccag gagtagggaa aaagacagca caacaaatga tcttggatct
    20641 aaaaggaaaa ttcggtgagc tgagtatcga tacacctttt agtctatttg atgaagcaaa
    20701 cacaaaagac gctacagctc tatctgaagc aatagaagct ctctcggctc ttggttacag
    20761 tgatcgagaa atcaaacgtg tagaaaaaca attaaaagaa gtagataatc agacaacgga
    20821 tgagtatcta cgccaagcac tcaagctaat gatgaaaaaa taaggaggag accaaatgac
    20881 agatgaatac ttgttatcac cagaatctgg cgaaaatgaa ctgagtttgg aaaaatctct
    20941 tcgaccacag ctgctttctc aatatatcgg acagagaaaa gtcaaacaag aacttcagat
    21001 ttacatcgaa gctgccagaa atcgggaaga acctttagat catgttctgt tgtacgggcc
    21061 tcctggatta ggaaaaacga cgatggccat ggtcattgct aatgagatga ccgtaaatat
    21121 tcgtacgacg agtggccctg ctatcgaacg tccgggtgac ttagtagcaa tcttgaatga
    21181 actggaagca ggggatgtcc tgtttatcga tgagatccat cgattaccta gagtggtaga
    21241 agagatgctt tattcagcta tggaagattt ctatgtagat atcatggtag gacaagggcc
    21301 aaccgcacat ccggttcatt tccctcttcc tccctttact ttgattggcg cgacgacacg
    21361 ggcaggtatg ctttctgcac ctttacgaga tcgatttggc atcatttctc atatggaata
    21421 ttatgacgaa caggatctgc aggagatcgt tttacgttct tcggatattt ttcaaacaga
    21481 gatcattgaa gaaggggcat tggaaattgc tcgccgatca cgaggaacgc ctagaatcgc
    21541 taatcgcttg ctcaaacggg ttcgtgattt tgcgcaagtc caaggagacg ggaaaatcga
    21601 tcgtcctata gcggatcagg cacttacttt gctacaggtc gatcaggctg gattggatta
    21661 tgttgaccag aaattgttgc gaacaatgat cgagttatat ggcggcggac cagtagggtt
    21721 aagtacgctt tcagtaaaca taggagaaga gcgtgagacg gtagaagata tgtatgaacc
    21781 gtatctgatc caaaaaggat ttttaaagcg tacacctaga gggcgaattg ctactgctta
    21841 tgcctatgaa cattttggtt acgactatca tatatgattt ttgcttttag attggaggaa
    21901 aatgaatgag tgatacaaca cgtacgaaac aattgatcaa aaacgtattg attgatttgt
    21961 gccaaacgca atcttttcga aaaatcagcg tccaaaaaat cgcaaacgaa gcaaaaatca
    22021 atcgacaaac attttattat cattttacag ataaatacga tttgcttcgt tgggtctatt
    22081 gtgaggatgc attgcattat ttagatccac aatgtctttc acttgataat tgggaagagc
    22141 aagcattgaa aatgctgaaa gcaattgctg agaagaaaga attttatgca aatactgtgc
    22201 tttctgaccc agaaatttta caaaaagagt tttctcaact gatccaaaaa tcatttatca
    22261 agatttttga acaaatggat gaagaaagac aactttctgc aagagacaaa ctattttatg
    22321 ctcgtttttt ttcttacggt tgcagtggtg tattagtcaa ttggatcacg aatgaatatg
    22381 aagaaacacc gatggaaatc gctatgcagc ttttccgttt agccaaggat acggaattat
    22441 attcttatcg tctttatgca aaagaagagt tggaagaaca gtaatagaaa aattttttgt
    22501 tggaaaagca ataatcgcta aaaggagatg gggaaatgac aaaaatttta tttgtttgtt
    22561 tgggaaatat ttgccgttca ccgatggcag aaggcttgct gagaaaacga atagcattag
    22621 aaggaaggga agcagaattt tttgtggatt ctgctgctac aagcacgtat gaagttggaa
    22681 agacaccgca ccctggaacg aaaaaaatat taaaccaaga aaaagtagat atgacgaata
    22741 tggttgctcg gcaaatcaca ccccacgatt ttgaaacttt tgattggatc attggcatgg
    22801 atcaagaaaa tgtagaagaa ctgaaacgaa gagcaccacg ctcagcgcag gggaaaatcc
    22861 atttgttttt aagtagcatt cctggaaaag aaaaagaaaa tgttcccgac ccatactata
    22921 ccaataactt tgatgagaca tatcacttga tcacagaagg cctgaatcat tggtggaata
    22981 tttggattga tgaaaataaa ataacaaata atttgtaaag ttaacgcttt ctttttaagg
    23041 cgatgttaga gcatttcctt gtttttttgt aaaatgttag gtaaaataaa attaattaag
    23101 taacactttc ttagctaaag gaggagtgca ttcgatgaac gaagagacag tatcgccaat
    23161 gaagaagctt acgaagttag ttactcatac ttcgtatcaa ctttttgatt cttttgtaaa
    23221 tgacactgaa gaacatgcta agaatgtacg attgcaagga aagtttattc ttgagctgca
    23281 aaaagctgct actgaacata gtctagttat cttacaagta agagcaaatc gtttgagtag
    23341 caagtatgaa acgatctcag gctgggtggc gacaaaaacg gtcgaaaaag accgagtgat
    23401 gatccgccta caaaacgaag agcagcagat ccggatggtt cctgtagaac aaattgaaaa
    23461 aataacaatc ctttcaccag caggggaaca ggaacggtta tcaaaataat ttataccaga
    23521 tagaaaagca cgtatgagct tgtaccatca atcattacta atcaaaaata accgaatgct
    23581 ttaagctgat atcagtaaaa cggaacgttt taggatgagt atcatagcat gacagcattc
    23641 ggttgattag ttgaatcgat tggatggtac aagttttttt gataaaaaaa ggtcctgcta
    23701 gccggggagc tagcaggata aggagttaaa aatgaaaaag tgttgttagg gttgtttgtt
    23761 ggtatgctta tataatagtc attagttgtg aatattttgt gacgaaagtt ttcgaaagtt
    23821 gttaagtttt tgagagtata aaatattttg tgtaaatgaa aaaatccata caaaaaagga
    23881 agtcccttct gtagaataaa gttaacgaca accaattcac agaaaagagg acttccctat
    23941 gaatgatttt actacagaaa ttgtgcaaac tctagtcact aaaggcgatt taaatgaatt
    24001 attccgttcg cacttagaaa aagcgataaa cacactccta cggactgaat taacggcttt
    24061 tttagattac gaaaaatatg atcgcactgg ttttaattca ggtaattcga gaaacggttc
    24121 ttactttcga tcaatcaaaa ccgaatatgg tgaattaaca ttggaaatac ctagagatcg
    24181 taatggtgag tttaaacaac aaactttacc agcctacaaa agaacaaacg atacattgga
    24241 aaccactatt atccattta
//
LOCUS       DARBEC010000040        23734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.040, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000040 DARBEC010000000
VERSION     DARBEC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.040"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            299..1960
                     /locus_tag="SCL80_001463"
     CDS             299..1960
                     /locus_tag="SCL80_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1107340.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="SCL80_001464"
     CDS             complement(2030..2377)
                     /locus_tag="SCL80_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEG1107341.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSSLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="SCL80_001465"
     CDS             2461..3402
                     /locus_tag="SCL80_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107342.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="SCL80_001466"
     CDS             3403..4446
                     /locus_tag="SCL80_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107343.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="SCL80_001467"
     CDS             4452..5522
                     /locus_tag="SCL80_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107344.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="SCL80_001468"
     CDS             5512..6465
                     /locus_tag="SCL80_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1107345.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="SCL80_001469"
     CDS             6477..7487
                     /locus_tag="SCL80_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1107346.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="SCL80_001470"
     CDS             complement(7772..8593)
                     /locus_tag="SCL80_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1107347.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="SCL80_001471"
     CDS             complement(8690..8968)
                     /locus_tag="SCL80_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107348.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="SCL80_001472"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="SCL80_001472"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEG1107349.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="SCL80_001473"
     CDS             complement(11724..12311)
                     /locus_tag="SCL80_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107350.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="SCL80_001474"
     CDS             12486..13808
                     /locus_tag="SCL80_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1107351.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="SCL80_001475"
     CDS             13963..14739
                     /locus_tag="SCL80_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1107352.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="SCL80_001476"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="SCL80_001476"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEG1107353.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="SCL80_001477"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="SCL80_001477"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEG1107354.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="SCL80_001478"
     CDS             16629..17258
                     /locus_tag="SCL80_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107355.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="SCL80_001479"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="SCL80_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEG1107356.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="SCL80_001480"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="SCL80_001480"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEG1107357.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="SCL80_001481"
     CDS             19445..19981
                     /locus_tag="SCL80_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1107358.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="SCL80_001482"
     CDS             complement(20021..21784)
                     /locus_tag="SCL80_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107359.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="SCL80_001483"
     CDS             complement(21787..23502)
                     /locus_tag="SCL80_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107360.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
BASE COUNT         7656 a         4333 c         4810 g         6935 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgaggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt gttaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atga
//
LOCUS       DARBEC010000041        23496 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.041, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000041 DARBEC010000000
VERSION     DARBEC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.041"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            2..74
                     /locus_tag="SCL80_001484"
     tRNA            2..74
                     /locus_tag="SCL80_001484"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:35..37,aa:Asp,seq:gtc)
     gene            78..150
                     /locus_tag="SCL80_001485"
     tRNA            78..150
                     /locus_tag="SCL80_001485"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:111..113,aa:Phe,seq:gaa)
     gene            155..225
                     /locus_tag="SCL80_001486"
     tRNA            155..225
                     /locus_tag="SCL80_001486"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:187..189,aa:Gly,seq:tcc)
     gene            238..311
                     /locus_tag="SCL80_001487"
     tRNA            238..311
                     /locus_tag="SCL80_001487"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:272..274,aa:Ile,seq:gat)
     gene            323..394
                     /locus_tag="SCL80_001488"
     tRNA            323..394
                     /locus_tag="SCL80_001488"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:356..358,aa:Glu,seq:ttc)
     regulatory      583..662
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            660..1853
                     /locus_tag="SCL80_001489"
     CDS             660..1853
                     /locus_tag="SCL80_001489"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEG1107361.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            1983..3455
                     /locus_tag="SCL80_001490"
     CDS             1983..3455
                     /locus_tag="SCL80_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107362.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            3538..4326
                     /locus_tag="SCL80_001491"
     CDS             3538..4326
                     /locus_tag="SCL80_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEG1107363.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(4393..5061)
                     /locus_tag="SCL80_001492"
     CDS             complement(4393..5061)
                     /locus_tag="SCL80_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEG1107364.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQIMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            5176..6888
                     /locus_tag="SCL80_001493"
     CDS             5176..6888
                     /locus_tag="SCL80_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107365.1"
                     /translation="MKLILRHAKKYKVAVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            6891..8660
                     /locus_tag="SCL80_001494"
     CDS             6891..8660
                     /locus_tag="SCL80_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010762530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107366.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENCKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFE"
     gene            complement(8865..9431)
                     /locus_tag="SCL80_001495"
     CDS             complement(8865..9431)
                     /locus_tag="SCL80_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1107367.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(9431..10387)
                     /locus_tag="SCL80_001496"
     CDS             complement(9431..10387)
                     /locus_tag="SCL80_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEG1107368.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(10387..11043)
                     /locus_tag="SCL80_001497"
     CDS             complement(10387..11043)
                     /locus_tag="SCL80_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEG1107369.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            11413..13431
                     /locus_tag="SCL80_001498"
     CDS             11413..13431
                     /locus_tag="SCL80_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1107370.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    13501..13707
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            13858..16272
                     /locus_tag="SCL80_001499"
     CDS             13858..16272
                     /locus_tag="SCL80_001499"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEG1107371.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            16620..18173
                     /locus_tag="SCL80_001500"
     CDS             16620..18173
                     /locus_tag="SCL80_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEG1107372.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(18554..20338)
                     /locus_tag="SCL80_001501"
     CDS             complement(18554..20338)
                     /locus_tag="SCL80_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107373.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(20353..22074)
                     /locus_tag="SCL80_001502"
     CDS             complement(20353..22074)
                     /locus_tag="SCL80_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107374.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            22174..22935
                     /locus_tag="SCL80_001503"
     CDS             22174..22935
                     /locus_tag="SCL80_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEG1107375.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
BASE COUNT         7432 a         4214 c         4871 g         6979 t
ORIGIN      
        1 tggttccgtg gtgtaggggt taacatgcct gcctgtcacg caggagatcg cgggttcgat
       61 tcccgtcgga accgtttggc tcggtagctc agttggtaga gcaatggatt gaagctccat
      121 gtgtcggcag ttcgattctg tcccgcgcca ttatgcgggt gtagtttagt ggtaaaacca
      181 cagccttcca agctgttgtc gcgagttcga ttctcgtcac ccgcttttag tttttatggg
      241 cctatagctc agctggttag agcgcacgcc tgataagcgt gaggtcgatg gttcgagtcc
      301 atttaggccc atttatttta ttggcccgtt ggtcaagcgg ttaagacacc gccctttcac
      361 ggcggtaaca cgggttcgaa tcccgtacgg gtcatggaag atactttttg agtatctttt
      421 tctttttatc tgaaagaatg attttaaaaa gcttagcaga gttatcttta tggatgattg
      481 tgctaagctt ttttggacag ttctaatgta aaatattttt atatttttta agcaaaaaaa
      541 tgaaaaaata cgagcagaaa actgttttat ctgctatact tggttcgttg caagttcccg
      601 aaaggatccg caggacggag atgccttgta accgaattta actaggggga atcattataa
      661 tggtagaaag acacttattt acatcagaat ctgtttctga aggacatcca gataaaattg
      721 ctgaccaaat cagtgatgca atcttggatg caattttaaa acaagatcca acagcacgag
      781 tagcgtgtga aacgtctgta acaactggtc ttgttttagt atttggagaa atatctacga
      841 cggcatatgt agatattcaa aaagtcgtac gggaaacaat aaaagaaatt ggttatacac
      901 gtgcaaaatt cggatttgat ggagatacag cagctgtatt ggttgcgatt gatgaacaat
      961 ctcctgatat tgctcaagga gttgatgaag cacttgagat ccgggatgaa gataaaaaag
     1021 acgtattaga tgagattggt gctggtgacc aaggtttaat gtttgggttt gctgtagatg
     1081 aaacaccaga attgatgcct ttaccaatcg ctttgagtca tcgtctggta cgacgcttgg
     1141 cagatttgcg taaatcaaat gaattaacgt atttacgtcc agatgcaaaa tctcaagtaa
     1201 cggttgaata tgatgatcaa ggacaaccgg aacgcgtaga tacaatcgtt atttcaacac
     1261 agcatgatga tgcagtagat aatgaaacaa ttcgtcatga tgtcatcgaa aaagtagtga
     1321 aggaagttat tccagctgaa ttattagatg atcaaacgaa atattatatc aatccgactg
     1381 gccgatttgt cattggtggt cctcaagggg atgccggatt aacaggaaga aaaatcattg
     1441 ttgatacgta tggcggttat gctcgtcatg gtggcggtgc tttttctggt aaagatgcga
     1501 caaaagttga ccgttctgcc agctatgctg cacgttatat tgctaaaaac attgttgcag
     1561 cagggcttgc tcgcaaagca gaagtacagc tagcttatgc gattggtgtt gctcaacctg
     1621 tttcgatctc aatcaatact ttcggtactg gaacggttcc ggaagaagaa ctgattgctg
     1681 cagtgagaga aaacttcgat cttcggccag caggaatcat cgagatgctt gatttgcgtc
     1741 gtccgattta caaacaaaca gctgcttacg gtcattttgg tcgtacagat gtagatttgc
     1801 cttgggaaca tacggacaaa gtagatgcgt tgaaagcaag tttagcaaaa taagttatat
     1861 tcagctggaa cgatcgccaa gtggtggttg ttccagcttt ttttatggca gttaacctct
     1921 tgaaaaagat agtaattatt tatctatttt tagatcctgt cataatgaaa gggaggaata
     1981 agatgaaaaa agaaacaaat gttactttgg tcacagtgag tattttcatt gcaacgttca
     2041 tgacggctat tgaaggtaca atcgtaacaa ccgctatgcc aacgattgtc ggttcattac
     2101 atggaatgga aatcatgaac tgggtatttt cgatttattt gctgacaaac gctatgctta
     2161 ctccgattta cggaaaactt gcggataaag tgggacggaa accaatcttt atttttggga
     2221 cattattgtt tattatcggt tcgttatttt gcggtttatc tcaaagcatg attgggttga
     2281 ttgctgctcg tgcgattcaa gggatgggtg ctggggcaat gatgccagta gctttgacga
     2341 tcatcggcga tctttattct attgaaaagc gagcaaaagt tttaggtttg aatagttcag
     2401 cctgggggat tgccagtgtt tttggaccgc ttgcgggagg attcatcgtt gaaacgatca
     2461 gctggcactg gatctttttt atcaatgtgc cgatcggaat tctattaatt attttgattt
     2521 ggctatattt aaatgaacca aaaatcatac atgagtctaa accaatggat atttttggta
     2581 gttttagttt gatggccatg cttgtctcat tgctgttagg gttccaatta ttaggagacg
     2641 aaggaatgag tccgcttgtt tctgctttgc tgctaggtag tatcgtaatg ttaggtattt
     2701 ttgtttttgc cgaaaaaaga gctaaagatc ctgtcatcat gatgcattta ttcaaaaatc
     2761 cactatttgt tgtggtcaac ttggctgcag cattggctag tggttttttg atgggaatcg
     2821 atgtttatat ccctatgtgg atgcaaggtg tattagggct gaatgcagca attggtgggc
     2881 tggtattagc accattatca ctagtctgga tggtcggttc tttccttgca agcaagtgga
     2941 tgactcaaat gaaaactcat tgggtcttga gaattggact actaatcgca gctattgggg
     3001 gcgcttggtt gtatcttatg cctttcagaa gtaactattt atggtttttt gctatatcaa
     3061 cggtcatcgg tataggctta ggtgtgacca tgactacctc gactgtcagt gcacaaaata
     3121 gtgtatctcg tgaccagctt ggtgtagcaa cttcttttaa tacgttagtt cgaacaattg
     3181 gtcaaacagt catggtttct atttttggtt tattgataaa tggggtaact aaacgagaac
     3241 ttgcagcagc aaatttaaca aatgatgcag atatcatgaa taaactggtg aatccacaaa
     3301 cggctaaata tattgatgcg gatattttgt taccattaag aaaaattctt tatgatgggt
     3361 tacattccgt ttatctagtt ggcttgttac tcgtgatcat tgctttggtc ttaacgcttt
     3421 tcgttcatga aaaaagtggt aaaataaaac agtagtttcg gttaagatat gtacgtagcc
     3481 gaatcattca aatttaaagt tattcaaaaa taacttctta ctaaggaagg cgaaaatatg
     3541 gcatttattc aagcaaatat ttattcgaat gtgttagaaa tggaagtcaa tctaaatgtg
     3601 atactaccgc aaacaacaaa aaaggtgatt ggcaccagtt cagagcaaga gacaacggat
     3661 atacctgttt tatatttact tcatggaatg ggtggcaatc atagtgtatg ggaaagacgt
     3721 acgtcgattg aacgttatgt agcaaactac ggattagctg tcatcatgcc atctactgat
     3781 cttggctggt acacagatac agtttatgac atgaagtatt ggacattcat tgcagaagag
     3841 ctacctgaga tttgtcatga attattcccg caattaacaa gaaaaagaga aaaaacatat
     3901 gccgctggat tatcgatggg cggatatggc gcgttaaaac taggccttgc aaaatcagaa
     3961 aatttcgcag cagtaatctc tctttcaggc gctgtttctg taggaaatcg aatggatgat
     4021 cttttgatgg ttcgtaaagc atctttttgg gaagggatct ttggaccatt ggatcaaatc
     4081 gaaggttcaa aaaatgatcc gcaatactta ttgaaagaac tagtaaaaag cggaaaacca
     4141 gttccaagat tttatatggc ttgtggggaa tctgattttc tttatcaggc aaatcaggaa
     4201 atggtatctg ctatgcagga acaaggcttg gcggtcacct ttgaacaagg acctggcgat
     4261 cataactgga tcttctggga ccaatggatt caacgtgcat tggcgtggct aatggaagaa
     4321 aaataaaaaa agagtcttaa aaagatagct cctatagaga tcacaaacat agtgatctct
     4381 ataggagctt ttttactgct cgttttctct tcttttattt tgttggcata agccttcaat
     4441 ataggtttgt gcactcgtta gaaagagtgc tttttcattc aattctgcat gggactgcat
     4501 actttcttta aatccctcta attgattttc aactacataa ttacgcataa atagaactgt
     4561 ttctttcaaa aattcatttc cgtccataat tttttgtcga agctgctgtt catgctgctg
     4621 atccattaac tgtccaatca ataaattgtg gagagaggca atggcctgat gactttgggt
     4681 gactgaaaat ccttcacgaa caagtaattg gatcatttgc tccaaatgtt ctaatcgact
     4741 cggaaaagcg ggcacagtat tgatttctaa ttctgcccca caagggaaac gtgtataaag
     4801 gtcatagtaa ttttccatat actgcagcaa ctgttcattc caaggcaaag ttggatctgg
     4861 aagcgctaat tcttcttcca ttgtttcagc cattatttgc aagagctgtt gtttgttttt
     4921 gaaataccaa tataaagcag gtgcttgaac atgaattctg tttgcgatgt ttcgcatgga
     4981 aagctgcgaa agagcttgtg tttcagccag gatatcaaag gcggcctgaa tgatgattgc
     5041 ttgagaaagc tttatttcca ttttatcaac ctcgtgtatt gcattatctt tttaaacatg
     5101 gtaaattgtt agtcggtcta attttacagt gttaaattag tcctgtcaat ttaaacaaag
     5161 gggtgtaatc aacgaatgaa attaatctta cgacatgcga aaaaatataa agtagctgtt
     5221 tttatttcct tgctgtccgt agcagtcatg gttgcagcag cattatggca acctaagtta
     5281 ctacagcaag tattggaagc tattattaca gaagataatg atgaaatgaa aacaattggt
     5341 atttacctga tcagtctggc tttgctagga ttagccgcag gtgtaacgaa cacgattttc
     5401 tctgcaaaag tagctcaagg cgtaagtgca gatatccgag aagaagcatt tcgaaagatc
     5461 cagacatttt catttggaaa tatcgaacag ttctctgctg gaaatcttgt tgttcgtcta
     5521 acaaatgata tcacgcaaat ccaaaattta gtgatgattt ctttgcaatc actttttcga
     5581 attccattct tatttattgg tgccttcatt ttagctatga tcacgatgcc gcaattatgg
     5641 tggatcatcg tagcgcttat cgttacggta tttctgatta cggcactttc atttacaagg
     5701 atgggcaagc acttcatgat cattcaaaaa ctgattgatc gtgtgaatag cattgcaaaa
     5761 gaaaacttac tagggattcg tgtagtgaaa tcttttgtac aagaagaaaa ccaattgaag
     5821 tcattcagca aagtaagtga ggacttaaca aaacacaaca tcatagtcgg tacattattt
     5881 tctgtgatga tcccgtcctt catgctggct gccaatttgg ctgtggttgg ggcgatcttt
     5941 tttgtgagcg atcttgttaa agatgatcct acgttaattg gtggaattgc ttcattcatg
     6001 aactacttga tgcagattat gatggcaatc attattggcg gaatgatgat gatgatgact
     6061 tcaagagcag ccgtatcgct gaagcggatt gcggaaattt tagatgctga accagatctt
     6121 acttatttag acgttccaga acaagagttg acgggttctg tcacttttga ccatgtttct
     6181 tttcgttatc ctggtgagga tacagatacg cttaaagata tcagcttttc tatcaaacct
     6241 ggagaaatgg ttggtatcgt cggggcgact ggtgccggga agtcaacact agcacaattg
     6301 atccctcgtc tatttgatcc gacagaaggc aaagttgaag taggtggtgt agatcttcgt
     6361 caagtaaatg aaaagagttt aagacaaacg gtttcctttg ttttgcaaaa agcaatttta
     6421 ttctcaggaa cgattgctca aaacttgcgc caagggaaaa agaatgcaac agaaaaagag
     6481 atggaacatg cttcttctat tgctcaagca aaagaattca tcgaaaagtt atctgatcgt
     6541 tacgaagcac caatagaaga acgtagcagt aacttttctg gtggtcaaaa gcaacgttta
     6601 tccattactc gtggcgttat cggtaatcca aagatcttga tcctagatga cagtacgagt
     6661 gcactggatg ctcgctctga aaagctggtc cgagaagcac tcgatcgaga tttgaaggga
     6721 acaacgacga ttgtcatcgc acaaaaaatt gcttctgttg tcaaagcaga ccgcatctta
     6781 gtattggatg aaggcagatt agtcggcgaa ggaacccatg aggaattggt agctggaaac
     6841 cgtgtctatc aggaaatctt tgaaactcaa aaaggaacgg aggagtaagt atgactgatc
     6901 taataaaagc aagtaaattt ttctatcact atttgaagcg gtacaaactc tcctttttct
     6961 ttatctttat cacagtgatc attgctacct atctacaagt aaaggcacct caatacatcg
     7021 gggaagcttt tcaagaatta gctaattacg taggaggctt gatgcaaggc gtagacgata
     7081 aaagcaaatt tgtttctgta atatggaaac tcttgctgtt ttatgtacta gctagtgcag
     7141 ccaactttat ctatagtatt ttgttcacac aagtagtagg gaagtcaacg aaccgaatgc
     7201 gtatcggtct attcaacaaa ttggaaaaat tgacgatccg tttctttgat tctcatcaag
     7261 acggtgaaat tttaagtcgt tttaccagtg acttagataa tattcaaaat agtttgaacc
     7321 aagctttgct tcaagtcatc acgaatgctg ttttacttgt cgggatcttg atcatgatgt
     7381 tccgccaaaa tgtagagtta gcttgggcta caattgcttc cactccagta gccgttttga
     7441 tagctgtctt gattatccgc aaagcacgga aatatgtgga tcttcagcaa gatgaagtgg
     7501 ggaaactaaa tggttatatg gatgaaaaaa tcagcggaca acgtgtcatc atcacaaatg
     7561 gcttacaaga agaaacgatt gaaggattct tagagcataa tcaaagtgtt cgcgaagcaa
     7621 ccttcaaagg acaagcttat tctggtctac tatttccgat gatgcaagga atgtcactgg
     7681 tcaatacagc gatagttatc tttttcggcg gctggttagc attaaatggt gatttggaac
     7741 gttcggtggc attaggctta gttgtgacat tcgtccagta ttcacaacaa tattaccaac
     7801 cactgatgca gatttcatct ggatacagca tgatccaatt agcgattaca ggggcaagac
     7861 gattgaatga aatatttgat gaaaaagatg aagtcaaacc agaaaactgt aaagcatttg
     7921 atggtgttga aaaaggtctt gcgttgcagc acgtagattt tggttattct ccagaaaaac
     7981 tagtcttgaa agatgtctcg atcaatgtgg acaaaggaga aatggttgct cttgttggtc
     8041 caacaggatc ggggaaaacg acgatcatga atctgctaaa ccgtttttac gatgtcaatg
     8101 atggttcagt agtcatcgat ggaatagata tccgagatta tgatcttgat gcattacgta
     8161 gacatgtagg aatcgttttg caagaatctg tattgttctc cggaacgatc aaagacaata
     8221 tcacattcgg caaaccggac gcttcagatg aagaagtcat caatgcagca aaacaagcga
     8281 atattcatga cttcatcatg acgttagaaa aaggttatga tacagagata tccgaagaaa
     8341 ataacttatt cagtacaggt caaaagcagt tgatcagtat tgctcgaacg atcatcacaa
     8401 atccatcttt attgattttg gatgaagcaa caagtaatgt agacacggta acagaagcaa
     8461 ggatccaaaa agcaatggat gaagcaatca aaggaagaac cagctttgtg attgctcacc
     8521 ggctgaaaac aatcttgaat gcagatagaa tcgttgtctt gcgtgatggg gaagtcattg
     8581 aagaaggcag tcatcacgag ttattagcag aaaatggatt ttatgcggaa ctttatcata
     8641 atcaatttgt atttgaataa agaggttgtg aaaaagctgt cctgcatcaa gtaataagaa
     8701 cagccaatca aaaacagctt ctcatgtttt tgatttggtt ggacttatta ccgcagatat
     8761 aagcttttga gcaccgtgaa taaagaggtt atgaaatcaa cgctctaacc tgagtattag
     8821 agaaaaaaac gcaaaacata tcatttatgt tttgcgtttt ttatttatct ttttttctca
     8881 atacaatata aaataggcgg ctggttcttt tggttgataa accgataatt cagcacatta
     8941 tatgtttcct gtggcagttt ggaacaaaaa gaatcaacca tatccagttc tttttctcca
     9001 ccttcatgac catagtagac gacaaggatc aatcgtccgc gaggcacaag tcgtttcaat
     9061 atttcttcca tagctgtgcg tgtagtctct ggcatagtga tgattgcttt atcacttttt
     9121 gggagatacc ctagattgaa aatccctgct ttgagatttt gttgttcagc tatcacttct
     9181 cctaacgttt cgtggccttg tagaaacaat gagacacgtt cttctaactg acgctgttct
     9241 agcctttcac gagtagcatc gattgcttgt tgctggatat caaaactata gacatgacca
     9301 gtttttccga tgtgttctgc tagaaaaacc gtatcgtggc cattgcccat cgttgcatct
     9361 acgacgtgat cccctggttg caagatttct tgcagtaatg tatggctgaa atgcaaagca
     9421 gtttgcaaca tcagacgctc acctttttcc gtacatcata ttttccttgg aagctatctc
     9481 tgcgaagaag ttcttcatca attgcattca agacttccca ttttttcaag ctccacattg
     9541 gtccaatcaa ggaatcccac ggcgcatccc ctgtcaaacg atggatgatg atctcttttg
     9601 gtatcatttc cagctggtcg caaatgacat tgacatagtc aggacggctc attaattgaa
     9661 ggcgaccttc atgatagtcc cgcaacattc tcgttttgcg catcagatgc atcaaatgga
     9721 gcttgattcc ttggatatct gaatctaaaa tggtgcgtct gacattttct ttcatcattt
     9781 ctaaactttc gcctggtaat ccgttgatca aatgcgtaca aacacggata ttatgtttgc
     9841 gtaatttagc cacgccatct aaataagttt gataatcatg tgctcggttg atcagttttg
     9901 aagtttcttc aaaagttgtt tgcaatccaa gttctaccca taaatacatc cgctcgttca
     9961 gttcagcaag gtaatcaact acttcatctg gcagacaatc cggtcgtgta ccgactgata
    10021 atcctacgac acctggtaaa ttgactactt gctcaaatcg ttctttgatt atttcaactg
    10081 gcgcatgggt gtttgtaaaa ttttggaaat aaacgatgta ttgttggaca tgaggccatt
    10141 tttgatgcat catgtcgatt tctttctgga attggattgg aagagggtct tcaggtgcaa
    10201 cgatcatgtc tcccgaacca gatacactgc aaaaagtaca accgccatgg gcaacagtcc
    10261 catctcgatt cggacagtca aatcctccat cgatcggcac cttgaaaatt ttcccgccaa
    10321 actcatttcg taaagcgtaa ttccaagaat gatatctttt atttccttct gaataaggaa
    10381 aagtcattaa aagtccttct ttctgtaaga gttatctatt tgttttcttt tttctctata
    10441 cactggataa gtcaggcaca gccaagaaag actgagtaag taaccgccga tgatatcagt
    10501 aggaaaatga acgcccagat aaatgcggct tatcccgaca cttactataa taaacatcat
    10561 cagcaattga agcaatgtgc gtaaggttct tttttgaaca aaagctggaa gcagaaatag
    10621 taaagaaccg taaagaatca tactgcctgc tgcatgtcca ctagggaaac tgtaacttgt
    10681 ttcaactact aagtgcgcca atgaaggacg ctctcgcatg aaaaatagtt tgatcaccgg
    10741 gttgatgatt ccagcaatta caataaatcc agaaacgaac caaaatgttt ctgccctttt
    10801 tttcttatac cacatccata aagccgcaca agcaaataaa atgatgacgg taagaggatt
    10861 accgaattta gtcataaaga gttggtagct gttcagatca ggataagaag ctctgacaat
    10921 gcctgtaaac cactgatcga aaccagaaag ccacgacaga tggaacttta cgatccagcc
    10981 caaaatgaaa aacaggacaa gaaagaagct accagccacc tgaaatgatg ttttatattt
    11041 catacaattc ctcctttgaa gcaatgactt attatagcat ttttggtcca gcgaaaaaaa
    11101 cggtaaagag ataaaaccaa tgtatgattc ctctttgaat aaccaatatt cttataaaaa
    11161 aaataactgt aatcggaaag acattttttg gtaatgcaaa tgaaatgtta gggaaataaa
    11221 atcagtgttt ttcgaaatcc ttttaaacaa gaacgtagac gtaggaagaa cttgaaaatg
    11281 aacagaagtt gttcattacg gtaaattaca atatgattac aaaaaacaac aaagctctta
    11341 aataactttt aataaatatt aagagaagtt ataattatta ataatagata aattgaagtt
    11401 ggaggataga agatggaaaa cattgcaaga aaagaacgga gacgtttaga acagaccaaa
    11461 cgttttcgaa aagcaaaacg aggggcagca atcgtgggaa ctgcgatggt aggctgttct
    11521 gtcgctgccc cattagtaca gccggttcct gttcaagcgg atgaaacacc tgttcagttc
    11581 ggcgcaagaa tcaatacagc ggcatttatc acagaaattg ctacttatgc tcaaccaatc
    11641 gctcaagcca acgatctata tgcatcagtg atgatcgcac aagcagttgt agaaagcggt
    11701 tggggcagta gtgcgttatc tcaagcacct tattataatc tttttgggat aaaaggcagc
    11761 tatcaaggac aaaccgtcta catggatacg cttgaatttt taaataatca atgggtaaat
    11821 aaaaaggagc ctttccgaca atatccttct tttgctgaat ctttcaatga caatgcctat
    11881 gtacttagaa atacttcatt tggcaacggt tattactatg ccggtacttg gaagagcaac
    11941 acgaaatcct atacagatgc gacagcttgt ttgacagggc gttatgcgac tgatcctggt
    12001 tacgcaggca aattaaataa tatcatcgca acttacggat tgacccaata tgatacacca
    12061 gctagcggaa acgcaggcgg tggagcgact acaggtggca ataatactgg aagtgcaggt
    12121 agtactggcg gttcaagcaa ttctggtaat acaaactcag gttcagcaac ttcgaccact
    12181 tatactgtcc aatctggtga ttctgtttgg ggaatctcaa ataaattcgg tatcacaatg
    12241 gatcaattga tccaatggaa caacatccaa aacaatttca tttatccagg acaaaaactg
    12301 acaatcaaag gtagccagga aaatggctct tcgacgaata attccggtaa caataccaat
    12361 tcttcaggaa atgctggtac atcaaacgga ggacaaacaa ctggtgcgaa atacactgtt
    12421 caatctggtg attctgtttg gaagatctca aatgatcacg gaattacgat ggctcaattg
    12481 attgaatgga acaatatcaa gaacaatttc gtttatccag gacaacagct aatcgtatca
    12541 aatggcggtt cttctaatgc tggagcaaat actggctcaa atactgggaa tacaagtggc
    12601 tctagttcgt ccagctcaag ttcttctaat acagcttcag gaacgaaata tacagttaaa
    12661 gccggagact ctgtttggag cgtctcaaat aaatacggaa ttacgatggc tcagctaatc
    12721 caatggaaca atatccaaaa taactttatc tacccaggac aacaactgac tgtttcaaac
    12781 agtggatcaa caaacagcac gtcaggtaat acaggttcgt caaccaatac aaataacagc
    12841 agttcagtta atactggcag cagttcgtct caaggcactt ataccgtgaa agcaggtgaa
    12901 tctgtttgga gcgtggcaaa taaaaacggc atcacgatgg atcagttaat cgaatggaat
    12961 aatatcaaaa acaacttcat ttatccagga cagcaattga tcgttaaagg tggaacttcg
    13021 gcaaatacaa atacaggttc aactacttct gcagcgaaac caaatacacc gaatacgtct
    13081 gctacgacaa gtacatcatc cactggtaat acgatgtata ccgtgaaagc gggcgagtca
    13141 gtttggagcg tagcgaacaa acatcatatc acgatggatc agttaatcga atggaataat
    13201 atcaaaaata atttcatcta tccaggacag caagtgattg tgaagaaagg ttcatctcaa
    13261 tcaacaaacc aacaatcagt tactggttcg aaaacctata ctgtgaaatc tggagaatct
    13321 gtttggagtg ttgctgacag tcatggaatt acaatggctc aactaatcga atggaacaat
    13381 attaagaaca actttattta tccaggtcaa acgttgattg tagctaaata aaatagctgt
    13441 tcgaagaaat aacgattgaa ttccacaaaa atatttgtta ctataaggct acttaaatat
    13501 gacaacactt gaaaaggaag tagtaaaaag actgaagttt cagagagcgc atggctggtg
    13561 agaatgtgca cggatgcttt tgaactcgcc tttgagttgc ccttgctgaa tacccgagta
    13621 agcaagcggc ggtgacggcc gttacaacgt tcgaggcctt ttcactaagg taaaattagg
    13681 tggtaccgcg tagattacgt cctataaaaa gagcaatctt tttataggat ttttttattt
    13741 cttttgtgaa gtttgagaaa acaaagcaaa aggaaataaa aataaacaga ttctgccggt
    13801 tttttcgtta tatttataat agaaacaagt gttttcaata gaacaggagg acagcaaatg
    13861 agttacaatc ataaagaaat tgagaaaaaa tggcaaaaat actgggcaaa aacgaatact
    13921 ttcaacaccc atgatgatcc tgaaaagcca aaattttacg cactggatat gtttccctat
    13981 ccatccggtc aagggctaca tgtagggcat cctgaaggtt atacggcaac ggatatcctt
    14041 tcacgtgtga aaagaagtca aggatacaac gtgctgcatc caatggggtg ggatgcattc
    14101 ggtctgcctg ctgaacaata tgcgctagat acaggaaacg atccagctga gtttacccaa
    14161 aaaaatatcg aaacattccg acgccaaatc aattctttag gatttagtta tgactggaat
    14221 cgcgaagtga atacaactga tccggagtat tataaatgga cacaatggat ctttacgaag
    14281 ctttatgaaa aaggtctagc ttatgaagca gaagtagcgg taaactgggt acctgaatta
    14341 ggaacagtta tttcaaatga agaagtgatc gatgggaaaa gtgaacgtgg tggctatgat
    14401 gtagtcagaa aaccaatgcg ccaatggatg ctgaaaatca ctgcttatgc tgatcgctta
    14461 ttggatgatc tagaattagt tgattggcca gaaagcatta aagaaatgca aagaaattgg
    14521 atcggtcgtt cagtaggagc aaatattgaa tttaaagtgg ctggtacgga taaatcctat
    14581 accgtattta cgacacgacc agatactcta ttcggtgcaa cttactcggt tcttgcacct
    14641 gaactagatc ttgttagaga aattacgacc cctgaacaaa aagctgctgt agaagcttat
    14701 atcgaagaga cagctaaaaa atcagatcta aagagaacgg atcttgcaaa agaaaaaaca
    14761 ggagtattta ctggtgctta tgcaatcaat ccagttaatg gcaaggaaat cccaatctgg
    14821 atcgccgatt atgtattatc ttcatacggc acaggagcca tcatggctgt tccagcacat
    14881 gatgaacgtg actttgaatt cgctcaaact ttcggactag aaattcttcc agttatcgaa
    14941 ggtggagatg ttgaaaaagc tgcttacaca gaagatggta cgcacatcaa ctctgaattt
    15001 ctagacggca tgaacaaaca agaagcaatc gataaaatga atgaatggct agaagaaaat
    15061 ggcgtaggga aaaaagaagt cagctatcgc ttacgtgact ggctattctc ccgtcaacgt
    15121 tactggggtg aaccaatccc aatcatccat tgggaggacg gaacagtcac agctgtccca
    15181 gaagaagagc taccattacg gttgcctaag acaaaaaaca tcaagccaag cggtactgga
    15241 gaatctccat tagcaaatat cgaggagtgg gtcaatgtcg ttgatcctgt aacaggtaaa
    15301 aaaggccgtc gcgaaacgaa tacaatgcca caatgggcag gaagctcatg gtattacctg
    15361 cgttatatcg accctcataa caaaaaagaa ctagcagatt atgaaaagtt gaagcgttgg
    15421 ctaccagttg acatctacat tggtggagca gaacatgcgg tacttcattt actatatgca
    15481 cgattctggc ataaattctt atacgatatc ggtgttgttc ctacaaaaga accattccaa
    15541 aaactataca atcaaggaat gatcttaggc gaaaacaacg aaaaaatgtc aaaatctcgt
    15601 ggtaatgtag tcaatcctga tgatgtggtc gaaacttacg gtgcagatac actgcgcatg
    15661 tacgaaatgt tcatggggcc attagatgct tcgattgctt ggagcgaaaa tggcttagaa
    15721 ggaagccgta aattcttgga tcgtgtatgg cgtttgatcg ttgatgagaa caataaaatg
    15781 cgtgaccgta tcactacatt gaatgatggc aagcttgata aagtgtatca tcaaacagtg
    15841 aaaaaagtga cagaagacta tgaaaatctt catttcaata cagcaatctc ccaattgatg
    15901 gtctttatca atgaagccta taaagtggac gctctgcctt atgaatatat cgaaggattt
    15961 gttcaactac tcgcaccgat tgctcctcac atgggtgaag aactatgggc aatccttgga
    16021 aatgatggtg gtatttctta cgcaccatgg ccaacatacg atgaagcagc acttgttgaa
    16081 gacgaagtag aagttgtttt ccaagtaaac ggtaaagttc gtgcgaaaag caatgttccc
    16141 cgagacttag gaaaagatga gctagaaaaa gcagcattag ccaatgaaat agtgcaagaa
    16201 tatattgaag gcaaaacagt cagaaaagtt attgcggtac caaacaagtt agtcaatatc
    16261 gttgctaatt aattggctct ttgtcaataa ggactgatga gttgtgcaaa atcaaatctg
    16321 ggccagaatg aacttcattc tgtctcagat tttttgttat ttcaaattga ttaattgatt
    16381 gatcaaaaag gttttgattt tcatgttctc aaacacgttc aatgtctttt tcttctgaat
    16441 ggtcttgtaa gagatctctg taatcatcaa atttttgtct attaatctta gtattttttg
    16501 atgtataaac tacctgaaat tgagagtaca taccaaattc ataaattttt tattagtggg
    16561 actagtgatt gtatggattt tgacgcataa tgaatgaagt atgagaaaag gagagacaaa
    16621 tggatcaaga actatttatt atcgaagtat tatcagctaa acaaaaaatt atcgagaaag
    16681 cgaaaaaaag agcgaaaaga ataatgttca agcttttaag cttagtattt ttctctggtt
    16741 tgcttttttt cggagctgta tcgttcctat atcgccatat gagtgcagta gtccgttcag
    16801 ctgaagggat gcaaaaatta aataatttgt atcacttttc gtggggagtt ggcggaataa
    16861 caattgttgt gcttattgtt tttcttctac ttatttggct ccgttaccat caattgtcgc
    16921 gtcatttcga tcaatggctt agtcaaagag cacaacagtt tttacataat aaaccaactt
    16981 ctcaagatga tgactatctt tattttgaga agacttcgac gagtcaagct tttcggatac
    17041 aaaaaagtga ttgccagtta ttgctgagat cacttgatga tttacctatc tacttaggcg
    17101 aaagcaaatc aaaattcgga atgaacacat tccatatttt tttactagaa aacgaaccca
    17161 gatgggaaaa aagccagtct tcgcctggtg gatacctgac aactaagatg agtacattag
    17221 tcactggttt tttcctcatt cttttagctg gaacaatcta ttatgggaga tacactcagc
    17281 caaaaatgtc ttctcatata aatgctgctt ctcacatgac aatgaactca actaacgata
    17341 cgcatataca aaagacaaat gaagggttgt tagctgatcc taagcaaaag gtaaatttga
    17401 caacacaaaa gataaatgat ctcaaacttg atacagagac gcactctctc tatatgacaa
    17461 cagatagtgg tgctaattgg gtatttgtgc cgattgattc gcaatggttg cgttttggcg
    17521 aatacaccct gacaacgggg ctgattcctg aaggttattg gatggacaac acatatgata
    17581 tttccgccga tttctcatgg tttatctatt cttctgggga tgatgtacgt ctgctgactt
    17641 caagagataa tggtaaaaca tggcaaacgt ctattatcac ccaatcagtt cagccgatac
    17701 gttatcgaaa agcggtattt tatggcaacg atggtctatt tatctgtaca tcagccagtg
    17761 ggatgtctgc agaaactttg tacatttaca ctactatgga tggcggaatc acttggataa
    17821 aaagtggaag tacaatgatc aatcagccag tacaaaatgt cagcttcgtt acaacttctt
    17881 taggttttgt ttcgacacgt gaaagtttgt tttatacaaa caacggagga cgctcattca
    17941 aagaatcagt tctgtcgatt ccggaaaact atcagacagg ggggatagat ctttttcaat
    18001 caccaaatga agttactcaa ataagcgcga atacgttaga gacaaaattt tatttgttga
    18061 aaaaaggacc aatcgatgca gggaaaatgt ttgcctgcct cttccgttca actgataacg
    18121 gggaaacatg gcaattcgtt cagcaactat cgcaagtaga acaaggagat taaagaagag
    18181 agattgtgag cctgacgttc agcaccgagt cacaagaaac aaattcaaaa aacagcttct
    18241 catatttgtt gaatagtgta actcgtgatt cggagttgac aagagcaaca agaagcgatt
    18301 ttgaagtata gcttctcgta ttttacaaga tcgtgtctta ttgccgatag gttgtctctt
    18361 gaacaccgtt tattcgtaaa aagggattgt gacaagtctt ttgtcactac ttaccgaacg
    18421 aaactgaagg agctatccaa aaagtatcat tagtattatt ctttgtgttt ctagtggcac
    18481 cactagtaca tttaaaaaag ctatcgatca tggactaatc atacatgatc gatagctttt
    18541 tagataatgt ttgttacgct atctgaaatt gactattata aagactggca taaagcgttg
    18601 gtgattggag tagttcagaa tgtgttcctt tttcgacgat atcgccattc ttcattacaa
    18661 ggatcatgtc tgcattttca atcgttgaca aacggtgtgc aatcacaaaa cttgtcctat
    18721 cttttgtcac agcttccatt gcttgctgaa tcaatgcttc tgttcgtgta tctacgctag
    18781 aagttgcttc atctaaaata acgacaggag gattcgctag aatgactcga gcaattgtca
    18841 ataattgttg ctgtccttga gataacgtgc cgttttcgct agaaatgatc gtatcatatc
    18901 cgtctggcag tgttcgaata aagtgatcac attgtgcgat ttcagccgct tcgataattt
    18961 gttctcttgt ggcatccatt ttcccgtagg cgatattatc agctaccgtt ccttcaaaca
    19021 gccaggtatt ttgtaaaacc atcccaaaag aacgacgtag ttctccacgt gaaagcttgg
    19081 taatatcaag accgtcgaat tcgatctttc ctttattcag ttcataaaaa cgcatcagca
    19141 gattgactaa agtcgttttt cctgcaccag ttggaccaac gatcgctacc gtttgttttg
    19201 gctttactga aaaatcgact tgattcatca atattttatc tgatgtgtaa ccaaactgta
    19261 catttttgaa agctattttt cctttcggtt gttctaaaag tcttgcatct ggaaggtctg
    19321 gaatttcatc tgtttcatct aatatctcaa atatcctatc gattgctgcc attgcggatt
    19381 ggatcgagtt gatcacataa gaagcggtag aaattggttc agaaatctga ttgatgtact
    19441 gaagataagc ttgtaagaag ccgatcgtca caccacctga tagaacaagc atagcaccga
    19501 ataccgcact tatgatgaag gcgagttgat taatgaaacg aatggccgga tagatagcac
    19561 agttcataaa ttgagcacgt ttaaaagcgt tgtattgctt tttatttact tcgtgtatag
    19621 cagcttctgc atgagcttgc tgattgaatg ttttgaccac tagattacca gctaagattt
    19681 cttccattcg attatttaaa acacccagtt catactgatt ctgttcagaa atctgtttgt
    19741 ttttgttcgc aatcttagtt gtaacaattg aactgccaac gatcagcaat aagacgagca
    19801 aagtaagctt gctttcgata taaaacagca tcagaccagc agcagctatt gttacgacag
    19861 aagtaaaaaa ttgattgata cctgtcaata acacttgaga aagttgattg agaccggtag
    19921 ttgtccgact aatgatgtca cctacttgat gattgtcaaa aaacgacatc ggtaatgttt
    19981 tgaatttttt tgtgatttct tttcgcaccc tcaatgtgat cttttcgctt aacgctgcca
    20041 ttgtccgttc ctgaataaat gaagtgatgc tgcttaagac tgaacagccg atcagcaata
    20101 agacagggaa caacaatgct tgtttgatat cactgaaaga aagctgtcca atacctgttt
    20161 ttcttatttc tgataagaga tcatcgatcc cgattcccat gatcaagggc atggctacga
    20221 ctaataggtt cccgaataaa ctgcatagaa acaatccgta aaaatattta tgttcaggtt
    20281 tgatcatttt ccaaaaacgt atcaaagcac gcgtgttcaa cgtttttttc ttttccatcg
    20341 tatcccttct ttctatattc cttgtgattt tgcaaactcc tgataagttt ggcaattaga
    20401 cagtagttct tcatgagtac cttgtccaac gatttttcct tcatctaaaa caatgatgtt
    20461 atctgcttgt tggattgtac tgaggcgttg agcaacaatc acgagagttt tctccgacat
    20521 gtgtttatga agggctgctc ttaatgctgc atctgtttta tagtctaaag cggagaaact
    20581 atcatcgaat acatagatct ctgctggttt gatcaacgct cgagcaatac atatccgctg
    20641 tttctggccc cctgaataat tagaaccgcc ttgagcaaca aagctgtcca atccgtcagg
    20701 aagggtgtcc actagttctt ctaattgagc gatcttgatc gctttagaca actcttctgt
    20761 tgaggcatat tcatttccca tctgcaagtt gcttaaaatt gtcccgctga ataagaaagc
    20821 cttctgaggg acataactga tgtgggagcg gatctgttct tgagtcattt gagtaagagg
    20881 aataccacca agggttactt ttcctatcgt agggtcacta agtctcaaga gaagtttcgc
    20941 caaactactt tttccagatc cggtcccgcc aacgatcgct gttgtttttc ctttaggtat
    21001 tgtgaaatca atatcttcta gaactggatc agagatttca gtatatttaa atgtgacatg
    21061 ttcaaaagca gctgcatttg tctgatcaaa ggttggttca gctactgttt tgatcgtctc
    21121 ttcctcaatt tcgatctctg cttgcagtat ttcttctatc cgtcgtaatg aggcaaacga
    21181 tttagggata atgaccaata ccatagctgc gatcatcaag taagcaagtg tcagcataga
    21241 gtattcgact acagcagtca cgacaccgat ttgtaatgct ccttctgcta caagatttcc
    21301 gccaaaccac aaaatagaag cataaacgat tcccatgagc aaaaaagcaa ttggagtgac
    21361 ccaagcaaat attcggttca ctttgatcat ttgattagca tagtcagcaa agctcttatt
    21421 cgttcgcttt tcttcatatg attgattatc aaaggcacgg atcatattaa tcccagtaaa
    21481 gaattctctc aatgtgatgg tgatccgatc catttttggc tggatactca aagataaagg
    21541 tgcccctcgt ttcatcagtc gataaacaat ccatacataa aaaacaacag agatcaatgt
    21601 aacccatgtt agtaatagag aataacgaaa catcatgaga atggtgaaaa cgcaaataac
    21661 cggtgctggt aagatcattt ggcaaaataa aacaatcatt tgctggacat tatcgacatc
    21721 attcgtcatt cttgtcaaaa gagatccaga actaaaacga ctgatatcct tcattgacag
    21781 ctgttggaat ttacggaaat atttctcccg cgtttggaat ccaaacctag cggaagccaa
    21841 tgctgaaaag taactagagg caactgcagc gattgttcct agaaacgcta caagcagcat
    21901 ttcaactcca atttttttta taactgaggc ttcgccactt gctatacctg tgtcgatcag
    21961 ctttgctact aattttggta cacctaatgt accggcgatt tctaaacaga tcatcacaaa
    22021 ggtaaggaga gcgagtcctt tgtttttctg taagaatgtt tttaagatag acatattatt
    22081 cctccttttt tcctgatact tttcgattct ataccttcaa gtaacttgaa ggtcaataga
    22141 aaaaagaata aactgaaaaa gaggtgagaa aaaatgaaaa aaatgtatac gattggccaa
    22201 gtatcggaac tatttgattt gccaaaatca acgatccgct attgggatga acaaggatta
    22261 atccgttcaa gtagacaaga agagaatgat tatcgattgt ttgatatcga tgatattttt
    22321 atgctttatg atatcgtttt ttatagaaaa ttagatatac cgatcaaaca gatgaagaat
    22381 ttatatggaa aaacattgac ggagttatat gagacacttg atgaaacaga aagaagaatc
    22441 cataaagaac tggtggtgat gaaacaaaaa caaaaagaga taagggagca aaaaaaacag
    22501 ttgaaattga tgatcgatac aaacgaggaa gaatttcctg tagaagagat cccttttgac
    22561 tgtatgatct caacggagtt tgaagatatt gtggaaatca aaaaatttct acctaactat
    22621 tcaagctttg ggatgatgag tatgccagct tccagttcac agacgatgta tggatttttt
    22681 atcgacccta gtgaagtaca tttatttact caagacgtga tttgggagaa aaaagatacg
    22741 gctgtttatc gtcgattctt attgaaatcc gagatgaacc atgcggaacg taataatatt
    22801 ttagaaatca gagaaagaat gtatgaaaaa ggatggaaaa caggagaagt gatcggccag
    22861 tatctattga cgaatacgga tgaaaacaat atcagaactg aatattatca tgcatggata
    22921 gaaatgaaaa aataggaaat atgtgtttaa gataactcga aaatgatggt tgttttagtt
    22981 ataaagaaaa attttctggt tcgattacgg gtttaatgtg ggggattatt agtcgtggat
    23041 agatagaatt ttagtttttt agaggaaata tgtctttaaa aatggaatgg ttgcaattct
    23101 tagcgatata tacgatgtaa ttgtcctctt aaaaaagtat ggaaaaataa ttgtattggg
    23161 tgttatgtat aattcatggt gaactgagac taatgagaag aaaaattcat attgttatga
    23221 gtgagattgt atcagatgag aatgataaat taaataggtg cataaaagca ttacacttaa
    23281 aatacattgt tgcttacatt attacaatat gttaataagg caaaaataaa tagatggatt
    23341 gaataaacat attttttaat caaatagtcc ctactgtgac tttcctcagc tatcggtgat
    23401 actaccgata gcattttttt gcttataaaa taattataag tatttagaga tttttcttct
    23461 aacattcttc aaacaagaaa aatcacaaag gttatt
//
LOCUS       DARBEC010000042        23067 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.042, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000042 DARBEC010000000
VERSION     DARBEC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23067
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.042"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(111..566)
                     /locus_tag="SCL80_001504"
     CDS             complement(111..566)
                     /locus_tag="SCL80_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107376.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(720..1928)
                     /locus_tag="SCL80_001505"
     CDS             complement(720..1928)
                     /locus_tag="SCL80_001505"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEG1107377.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNEGAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(2040..2372)
                     /locus_tag="SCL80_001506"
     CDS             complement(2040..2372)
                     /locus_tag="SCL80_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEG1107378.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            2520..3395
                     /locus_tag="SCL80_001507"
     CDS             2520..3395
                     /locus_tag="SCL80_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1107379.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(3477..4058)
                     /locus_tag="SCL80_001508"
     CDS             complement(3477..4058)
                     /locus_tag="SCL80_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEG1107380.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(4064..5323)
                     /locus_tag="SCL80_001509"
     CDS             complement(4064..5323)
                     /locus_tag="SCL80_001509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEG1107381.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(5325..6371)
                     /locus_tag="SCL80_001510"
     CDS             complement(5325..6371)
                     /locus_tag="SCL80_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107382.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(6606..6881)
                     /locus_tag="SCL80_001511"
     CDS             complement(6606..6881)
                     /locus_tag="SCL80_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEG1107383.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCL80_001512"
     CDS             complement(7124..8434)
                     /gene="der"
                     /locus_tag="SCL80_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEG1107384.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCL80_001513"
     CDS             complement(8616..9845)
                     /gene="rpsA"
                     /locus_tag="SCL80_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEG1107385.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(9980..10660)
                     /locus_tag="SCL80_001514"
     CDS             complement(9980..10660)
                     /locus_tag="SCL80_001514"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEG1107386.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(10709..11344)
                     /locus_tag="SCL80_001515"
     CDS             complement(10709..11344)
                     /locus_tag="SCL80_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1107387.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(11410..12813)
                     /locus_tag="SCL80_001516"
     CDS             complement(11410..12813)
                     /locus_tag="SCL80_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEG1107388.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATKQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWETILLNLFKKNMEH"
     gene            complement(12800..13831)
                     /locus_tag="SCL80_001517"
     CDS             complement(12800..13831)
                     /locus_tag="SCL80_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107389.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESEVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            13862..14083
                     /locus_tag="SCL80_001518"
     CDS             13862..14083
                     /locus_tag="SCL80_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEG1107390.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(14248..14985)
                     /locus_tag="SCL80_001519"
     CDS             complement(14248..14985)
                     /locus_tag="SCL80_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEG1107391.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(15138..16478)
                     /locus_tag="SCL80_001520"
     CDS             complement(15138..16478)
                     /locus_tag="SCL80_001520"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEG1107392.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(16715..18238)
                     /locus_tag="SCL80_001521"
     CDS             complement(16715..18238)
                     /locus_tag="SCL80_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEG1107393.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(18616..19227)
                     /locus_tag="SCL80_001522"
     CDS             complement(18616..19227)
                     /locus_tag="SCL80_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1107394.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(19350..19475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(19557..20273)
                     /locus_tag="SCL80_001523"
     CDS             complement(19557..20273)
                     /locus_tag="SCL80_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEG1107395.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCL80_001524"
     CDS             complement(20279..20848)
                     /gene="scpB"
                     /locus_tag="SCL80_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEG1107396.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(20832..21629)
                     /locus_tag="SCL80_001525"
     CDS             complement(20832..21629)
                     /locus_tag="SCL80_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEG1107397.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFGTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(21599..21970)
                     /locus_tag="SCL80_001526"
     CDS             complement(21599..21970)
                     /locus_tag="SCL80_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1107398.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCL80_001527"
     CDS             complement(21989..22876)
                     /gene="xerD"
                     /locus_tag="SCL80_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEG1107399.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT         6470 a         4926 c         3910 g         7761 t
ORIGIN      
        1 aaaaatcttg tcacagtccc ttattctgaa taaacggtgt ggaagagaca gcctatcagt
       61 agtaataaga cacatctctt tcacagcctc atttttgcgt aaaaatcgat ttattcgaaa
      121 aattccccgt atacatcttc tacacgttga tctggattga tcacgatata caagttacct
      181 gttttttcgc tgatttttgt tgattgatat tgattgtcta atccttctgt tcctttatct
      241 agatatggga agtacacaag atcaggtgtg ccatttcggt aaaggatttc cattcgctcg
      301 atatcttcgt atttcaatgc gcgattaaac atgcccattt ccagtccgcc taggttgata
      361 tcatgtgtcg caactgcgtc cccttcatgg aagatttcga ttttaaatcc agcacacgga
      421 tggatttcaa caaattcact gccattgatt cgtccaaaac ttgttgtgat ttgtttgatc
      481 caaagatcgc cgatatgacg gcggttgatc gtccaggttt ctccatttct aaaataaaat
      541 tttagcgctg tcatttcttt actcattaaa aagactcctt tatgatgtaa tttattcttg
      601 tactacctca gtttagcaca aatttatgta agtggtcaca tcttatgttt tttcttctaa
      661 tgacgtatct gcccacttta agtagccaac aaagctgagc agatacagtc tagtgttttt
      721 taaatcaatt tttctttcga taaaaaatcc aaaacctgtt ttttatcatt ttttattctt
      781 tgttctacga cggctttttc agctaattgc aaagcttcac taacccactt accaggtttt
      841 tttccagttt cttctagcaa gattttacca tctactgcta attcgcttaa tgacttgata
      901 ggtaaacgat cataggattc tcctacggca tccatctgag gtgtttcgtc aaaatagatc
      961 atcaattctt ctacttgcag tgcatgtttt ttgcctaaga agtacaattc gaatggttcc
     1021 aaagctccag tttctcgttg tcgtagtcct tggacaagtg cttgaacgtc tttaatcatt
     1081 tggttagaac acttccacga tttcaaaaag gaacgaatct cattttcttt tagatccagt
     1141 tggctgatca ataatgccca tgcttggctc tcttctttca atgggacgga tggtaattca
     1201 gcaaaacgta aaagtgcttc gccttcttct ttcaaaccgg gacagcaaat gtagcactct
     1261 gtttcaataa atgcatggat tcccgcggcc ccttcatttc ccaagagcat cttgatgaat
     1321 tccacattta tacgttctac agatatttta cctaatagct cgtggttttc tttgatggcg
     1381 tagaaggttt cttcctctaa tttgaaccct agttgactga caaaacgcaa cccgcgcatc
     1441 attcgtaagg catcttcatg gaaccgctca tgaggattgc ctacagctcg caagatctgt
     1501 tcttctagat cttgcagacc atcgaacaaa tcgatgattt ctccatcttc tttcaaagca
     1561 aacgcattga tcgtaaaatc tcttcttttc aaatcttctg ccaacgaacg aacgaattcg
     1621 acatgatctg gtctacggta gtcctgataa gttgattccg tccgaaatgt ggtgatctca
     1681 tattgttcat cattttctaa taccaacaca gttccatgtt cgatcccaat atctatcgtt
     1741 tttggaaaaa gctgtttgat ttcttctgga aatgcgcttg ttgcgatatc cacatcatgg
     1801 attggctggt tcagcaagat atcacgaaca cttccaccta caaaataagc ttcatatcct
     1861 gcattctcta attttttcag aactggaatt gccttttggt attccagagg taattgtttt
     1921 aatttcattt ttccaacctt tcctgcgtgt ttcttgtcct gagataaaga ctcagttgca
     1981 tggtgactgt ttgacaagtc cgtttcagag tcttcaaggc caggaccact tcatcttgtt
     2041 tactcgtcct tacgctcaaa gcgattatgg tctctttgat tgaatttttc catgtttttt
     2101 tcaaaaactt cagttaagtc gatattcaat gaattagcca tgatcatcgt aacgaataaa
     2161 acatcaccaa gttcttctgc taccgagtta actggttcag aagccttttt gcttttttct
     2221 ccataagtgt ggttgacttc tcttgccaat tcacctactt cttcagttaa tcgagccatc
     2281 tgagctaatg gagagaaata ccccaccttg aattgctgga tatattcatc cacttcctgc
     2341 tgcatactgt ataaagaacg ctttttatcc atctttttcc ctccaaaact atcttaccaa
     2401 aactttaacg caaatcctag ataagagcaa ttgagaaaat cgaatttcca tgctacattt
     2461 aggaaggaat ttctaaatgg aaattctgtg aaaatacata attgaaagct ggaattcata
     2521 tggtcaaacg aaaaacagta aaagaagttc tttttatcat tataggaaca agtatttatg
     2581 catttggtct tgtttacttg aacatcgcta atcatttagc agaaggggga gtctcaggga
     2641 tcaccttgat cttaagagct ttatttggta ttgatccagc ttatacgact ttattaatca
     2701 acatcccatt gattttacta ggaggaaaga tcctcggtaa acgttccttg gcctatactg
     2761 ttctaggtac actttctttg tcattctttt tatggatctg gcaaagggtt ccactagaaa
     2821 tcaatttaca gcatgatcta ctgatcgttt ctctacttgc gggtttagtt gcaggtgtag
     2881 gaagcgggat cgtctaccgc atgggtggaa caacaggcgg cagtgatatt atcgctcgga
     2941 ttttagaaca aaattacggc atcactatgg ggcgttccct attggcattt gatatcatcg
     3001 tcttacttgc ttcattaacc tatatcgact tgaaacgtat gatgtatacg ttgattgttt
     3061 cgtatgtttt cagtcgtgtg attgattcga tcttagatgg cggatattct gcaaaaggtc
     3121 tacttgtcgt ctccaacaaa agcgaagaga ttgcaccact actgatgacc ggtctgcaaa
     3181 gaggtgtgac tttcctttct ggagaaggcg cattctctgg taattcaaaa aaaatcatct
     3241 atatggtcgt tagctctagt gaattaactg aagctaagag gatcgtccac gagattgacg
     3301 aaaaagcttt cctttcaatc atcaatgtgc atgaagtgga agggcaaggc tttacttatt
     3361 tgaagccaca gacaagatta ctgaaaaaac tataagaaaa aagacaaaga taggctatga
     3421 ggaaagattc cacatccagc tgacctttgt cttttttagt actatactat ttttaactaa
     3481 ttgttatgtt cacttacccg gttacttttc ttttgtgtct gcaaaaggat ctttcggtta
     3541 acttcatcag ataatttcaa aaaagcatcc ctatctccat cttctagtgc ttgatcgatt
     3601 tgagcataaa gcatttttag ttttgcctct tcttcttttt cttgaaaata tcgttcaatt
     3661 aattcagtat cttcttcact gactgaagaa tcccaagaag aaaatggatt gtcttctaag
     3721 atagatagat attctcttgt ctgccaagca ttttcgaata tacattccac atataaaggc
     3781 tttttccaat tcaaacggat ttcatgaaaa atctggtctg tgtccgtaaa ttcttttccc
     3841 tgcaaaaaca atttcatggg ttccccttca acagatagat cactgatctg cagtcctcga
     3901 tccgcaaaat tcgccccttc aacaaagtgg acattgctta aaatcgcttc atgattggca
     3961 agataattca agatccaaaa aacttctctt cggctaaaag atacatgatt gaccatccac
     4021 gttaaaaaat tcttcttttc ccgtacatca attagcattt ttctcacctc tctgttaggt
     4081 catctaataa tgacagtgcc tccatatctc ctggttcaag tgttaaataa taagtcaata
     4141 attcttgcgc tcttttcagc tgcccttctt ctcttaagaa aaaggcatac tctttcaaga
     4201 aatctggttc atgtgatagt tcatgataag cctgttcata gtgtacagct gcaacggtaa
     4261 agtcttctaa ttcattataa gcatgggcta ggttccattc tgcataagga ttagcagttt
     4321 cttccatctg attgatcact tgtatcacat cttcatatct ttcatcatcc aagtaaagat
     4381 tacttaaagt aagtaatgtt tcatcttgtt tgtctcctac ttctaaagct ttcagtaaaa
     4441 gttcttctgc tcgctgtcta tcatgcagac ggaaagcatt ttccgaggcc aggtgataaa
     4501 gttcgacttg gtaaggattt tctttgattc cttcttctaa cactgtttgt gcttcttcaa
     4561 tcatttcttc ttcttgaaga gcttgtccta gatatagata taacgattga taatgcggat
     4621 taacttctcg aagttcttcc agatagttga ttgcctgctg attttctttc aattgaagat
     4681 tcgtaaaagc tagctggaac agtcgatcat ctgtcctttc ttctttcagt gcttcttcta
     4741 aaggtacgat agctgcttcg aattctcctt gcatacttaa ggattgtcct aaacgttcct
     4801 gcatcgagat tcctgaaatt tcatttatcc cgcttgccaa caatgtttca taaaccactg
     4861 ctgcctcttt gaaacgatca actgaaaaat acaattcagc tagcgcaaac tggatcaatg
     4921 gttcatcagg aagcaaccca gctgcttctt tcaattttgc ttcactcact tcagggattc
     4981 cgataacttg gtacaaatca gctatcgcca ataagctttg cggatagtaa tcactttgct
     5041 tatccacttt ttccaggtat tcaaaagcta agtcgatctc attatcttcg attgcaattt
     5101 ctgctaacgg gatgttcagt ccgtcatttt ctgggtattg gttgatcagt tgttcaaaaa
     5161 tctgttttgc ttcgatcaaa aagccaatag ccagcaattc ttcgcctagt tcagctaata
     5221 tatctgctgg gtcttctttt aatgcttttt ctaaaaagat ttgtgcttgt gacaagtctt
     5281 catctgataa agcttgcaac attttttcgc tatttgtatt catttcaatt ccctcttatc
     5341 tcactgacga tttgttgaaa taccgtcatt gttctcaata attctttttc gtttggatgt
     5401 tggtatccgt ctattatacc tattttttct aaaacaagta tttttttgct tttttctgcc
     5461 aaaaaaagaa ctttttccaa atactccatc tttgaaatct gttccagcca gtggattgga
     5521 tagccaagca agtccagcca cacaaaaaag ttttttagtt ggaaatcaaa aggatatgct
     5581 tggagattcc acacgaaatg aaacatcatt cctaaaaatt ttttcatact agcagaaagt
     5641 agatggccat tttcggtcag ataaaaagct tgttcaaaca gtttaccata atttgacagg
     5701 ttttctgcct ctaattgata aaaatagatc atttcttcga tcatcccggc aaacggccga
     5761 gtcgttaatt gctttttatt tggatagttt ttatacagat tttgcaaaaa acgatgatca
     5821 caaatcattc cgcaacagat aaaagtcaaa aagtcaatct gctttccgtc cacctgccgc
     5881 tcgctgattg tttgatcata aacgatgcgt tctggcagat tgatggtttg cagcactaca
     5941 cgatttggta gctgcaaaat cgtccgctgc ggtgttaatg ctcggcttag agaacggatc
     6001 gatacaggga tcatccatag tgatgaatca agggttgtat gtttttgaaa aaaacctgcc
     6061 agatccatga tcccttcatt tccaaatccg atgataaaca tggattgatt ttcgggatag
     6121 cgtttgacga aatcaagcag gttctttaat tctgttaaat gattgcattg agtgttggca
     6181 caaatatacc agtcattgtt tgcttgattt gcaaataacg ggttgatctt atctgcaaac
     6241 aaatcataat agcgctgatt cgttaaaaat aagattggct gatcaatgga aagttctctt
     6301 agctgactga ctagcgtttc tccgtaaaac actttcgtca acgcatcttc tacttgataa
     6361 tcaagttcca tacgcatccc tcctcttctt atttttattt taccattagt aagcaaattc
     6421 tagaaaagat aagagcaaag ctcacctcat aacttggaaa taaataaaaa ttaaataaaa
     6481 tacatgtgtt ttctatgtat gacatacgga ttcggaatct ctgtctctta tttattcctt
     6541 ttctgctagg aaataaaaag aaaagcactt gtagccctag agccacaagc gctttttcaa
     6601 ctgtcttatt taacagcgtc ttttaatgct ttacctggtt tgaatgcagg tactttgcta
     6661 gctggaattt cgatttcttc accagtttgt gggttacgac ctttgcgagc tgcgcgttcg
     6721 cgaacttcaa agttaccaaa accgattaat tgaacttttt cgccgttagc taaagcatct
     6781 tgaatagttg aaaatacagc atctactgct gcagttgcat cttttttagt taaatcagta
     6841 gctgaagcaa ctttttcgat taattctgct ttgtttgcca tggatagatt cacctcctcc
     6901 acaagagata tgatagaaat atcatttagt taaaactttc tgagtggtcc agaagtttaa
     6961 aaagaaatat tgattaactt caatattctg ttatcacgat atcatatgaa ccgcttaata
     7021 gcaaggtttt taccaatttt cacatgcttt ttttagtcat agaaacgtaa caaacgaaac
     7081 tgtctttttc ttttgaaaaa aaaacgtcga tatggtaaaa tgcctatttt ctcctacgag
     7141 ggataattcg gatggacgtc ccttcaaaag taaatgcttt tctgatttga ttttctaaga
     7201 aacgttcata agaaaaatgc atcaattcct cttcattgac aaaaacgaca aatgtaggtg
     7261 gtttgacagc gacttgtgta gcataaaaaa tcttcaggcg ttttccttta tctgttggtg
     7321 ttggattgat tgctacagca tccataatga tatcattcaa cacagccgaa gagatacgaa
     7381 gattctggtt catacttact tgttcgatca tttcaggcag acgttccaac cgttgtttag
     7441 tcactgcaga aacaaaaacg attggagcgt aatcaagata ccggaattca tcacgaatct
     7501 cttgttcgaa atcacgcatt gtattggttt ctttctttac aagatcccat ttattcacga
     7561 cgataataat tccacgacca gcttcatggg cgtaacccgc tactcgcttg tcttgttccc
     7621 gaatcccttc ttcagcattc agtaccatca aaacgatatc agaacggtca atggcacgca
     7681 ttgcacgcat cacgctgtat ttttcagtat tttcgtatac ttttccgcgt ttacgcatac
     7741 cagctgtatc tatcattaag aatttctgac cactttcaga aacgaagtgg gtgtcgattg
     7801 cgtctcgggt cgttccttca atatccgaaa caattacacg ttcttctcct aaaatcgcat
     7861 tgatcaaaga ggatttacca acatttgggc gaccaatcaa actaaactta atcgtgtcgt
     7921 cgtcctcttc ttcagtctct tcagagaaat acttcactgc ttcatctaaa atatcaccaa
     7981 tccctagtcc atggcttcct gaaacaggat aaggatcgcc tagccctaag gaataaaatt
     8041 catagatatc gtttctcatt tctggattat ctactttatt gactgctaaa atcactggtt
     8101 tattacttct gtataaaatt ttcgcaacca tctcatctgc gtctgtcacg ccttcgcgtc
     8161 cgctgactac acaaatgatt acatctgctt cttcaatcgc gatttctgct tgatgcttga
     8221 tctgatccat aaaaggttca tcgccaagat caattccgcc tgtgtcgatc acgctgaatt
     8281 ctcgacctaa ccattcacct tttgcataaa tacgatcacg agttactcca ggagtatctt
     8341 cgacaatcga gatacgctca ccagcgatgc ggttaaaaat cgtagactta ccgacgttcg
     8401 ggcggccaac gattgcaatt gttggatttg ccatattatt tcctcctcac aagtttccaa
     8461 tctcacttag tttaacgaat gcattgccaa ctttcaagca tagattgtca attaaacgaa
     8521 gtatttttat tccttattga aatactaatc gcttttcaaa agaaaaactg agacaaaagc
     8581 agctgctttc atctcagtca cagatacaaa atcaattatt tttcagaatc atcggttgct
     8641 ttttcttcta cttcttcttt caacgcatca cctaaaatgt ctcccatagt aaatccagta
     8701 ttttcttctg gtaattcata agactcaaca ttttttggtt cttcttctga agctggtttt
     8761 tcttctaaag ctttgatact caatgcaata cgatgttctt ctggatggat ctctaatact
     8821 ttcactttca cttcgtcacc ttcatgaagc acttcatgtg gtgtcgcaat atgtttatgt
     8881 gaaatttgag agatatggac tagcccttct actccaggga acacttcaac aaaggcaccg
     8941 aagcttgtca aacgtttaac tgttccgtct aatactgtgc caactggagc ttttgtatcg
     9001 atttctgacc aaggtccagg ctgtgtgtct ttgattgaca aggaaacacg ttcttgttca
     9061 ggattgatag acaacacttt gacatttaca tcatcattga cagccaatac atcacttggt
     9121 tttgctacat gactatgtga aatctctgag acatggacta gaccgtcaat tccgcctagg
     9181 tcgacaaatg cgccaaaatc agtaagacgt gcaacacgtc cactgagaac atctccctca
     9241 tggatagttg aaagaatttc ttttttcttt tcttctcttt cgttttccac taccgcttta
     9301 tgcgataaga tcaaacgatt ttctgatggt tcgatttcga tgattttaaa gctcaatgtt
     9361 ttgcctttat agtctgaaaa gtctgcaaca aaatgatctt ccaccattga tgcagggaca
     9421 aaaccacgta caccgacgtc aacgactaaa ccgcctttga caacatttgt aacaggtgct
     9481 tcgatgacct tgccctcttg gaaatctctt tcgatatctt cccaaacttt tttagcatct
     9541 aaacggcgtt ttgaaagtag gtagctgccg ttttctttat ctttgccgat cgtactgata
     9601 acgactaaat ctaatacatc accgactttt actacttcgc tgatatcttc taccggcaaa
     9661 gttgataatt cttttgccgg aacgactcct tcaactccag ccccttcgat tccgacaatc
     9721 acttgtttgt cttcaatgac taaaacttcg cccttaacaa catcgccaac ttttacttct
     9781 tgtacgcttt tcattgcgtc ttccattgac tggttgttct cttgattttg atcaaattct
     9841 gtcataacac ttgtcctcct aaccaacatt ctgccgtaag tacggtactg tttttagttt
     9901 aattgattat tcaaaaaaaa taaagcgatt tcgttcgttt tccttgaaaa aacaattgtt
     9961 tttcattttt tttcttaatt taaaaaagaa gataaccttt tttcaaaaca acagctttta
    10021 ttgctgcaac gacttctgga atcgatttgc cagtcgtatc gatacgaaca gcatcctctg
    10081 cttgcttcag cggagagacg tcccttgttg aatcataatg gtctctttcc tgtatctctt
    10141 tcgtcagtgt ttcaagatca gtcggaattc ctttttcttg gttctctttg aaacgtcttt
    10201 ctgcacgctc acttacgctt gctactaaaa agattttcac ttctgcattc ggtaatacag
    10261 ctgtcccgat gtctcttccg tccatgacaa cgccaccgcg ttcccctatt tttcgttgga
    10321 cagcaactaa ttcttctctg acttttccat gtgcagagac ttcagaaaca gcttttgtga
    10381 catctggttg acggatttct aaagtcacat cttttgatgc tatgaataca tgctgtccat
    10441 cttcttgttg cgaaaaagag ataggatact ttttgatcag cgaaactagc ccttcttcat
    10501 ctgaaaaaga tacattgttc gtaatagcta aataagttac tgcgcgatac atggcaccgg
    10561 tatctgtata aatataattc aattcttttg ctaatatctt ggcaacggta cttttcccag
    10621 atgaagcagg cccgtcgatt gcaatactga tttgtgtcat gatataactc ctttttctca
    10681 gaaaaaaagc cgcaaagcgc ggctattatt atttaattct taattcttga ccagggtaaa
    10741 gcataaagtt gtttggatct aagccattca attcaaacaa ttgatcagtg gtgatccctg
    10801 cacgctcagc cacttgtttt ggaccttcgc ctgcttgaac agtcgttgtt gaaccggctg
    10861 cagctgatga agaaggtgtt gtttctgctg cattgctgct tgaatcccct gcttgataag
    10921 ttgcctcact tgaggaagtc ggtgttgtct ctgctgcaat actgctttct gacgttacac
    10981 ctgtatcttg agacacagta gattcttccg cgattgtact agattctact gttgaagtct
    11041 cagttgactc ctcttttgtt gtacttgtag aactttctac tactgatgtc gaagtttcag
    11101 aagaagttgc tgctgttgca ttgttgcttg atccatttct gatccagaaa taagcacctg
    11161 ctggaatagc gatacataag accagtaaaa tcaaaaatag cgaaagaaat aatgtatttc
    11221 ctttcttttg ttgtctctga gaacttcttg atgaaacttc atcatctgta tcgtagattg
    11281 gttgttccca tggttcttgt gtttcgttat tttctgtaga ttgatgtcta tcttttctgc
    11341 tcactgtttt acctcctaaa ctctatcgtg tgtattatac cataggaaaa agtgtcttgt
    11401 cctaaaaaat caatgttcca tgtttttttt aaataaattt agtaaaatcg tttcccaatg
    11461 aagttgttca gtcttagaaa aaagcttcct ctttggcaga agttctgctt ttgctccatc
    11521 aatatcacaa caattctgcg gcttttcgga gagtttttcg ccaaaataag ctaagatgaa
    11581 ttttctgcgg cagttttctt cattaatata gcgaagcatc tgctgcaggc ggaattcttt
    11641 ttctttttcc tgacgtttga gccgttccac ccaattttct ggtttttcag actgctggat
    11701 cagctccatc catttttttt gcagctcatc aaagggagct tgctttgtgg catattccaa
    11761 ataaagctca aaactttgtc gctgttctct agaaagttga ttaaagaaat aatggatacg
    11821 ttcatcccca ctttgataaa aaagaattgc tgcactttct tcctgatctc tgcctgctcg
    11881 accgatttct tgtacatagt tttctagact gtctggcaaa tcataatgga ccacataacg
    11941 gatatcagat ttatcgatcc ccataccaaa tgcattcgta gcaatgagaa actgcagctg
    12001 attttttaca aactgctgtt gaagttgtct tctttgagct gcatcaagac caccgtgata
    12061 gtatccaact gtaaaacgtt ccctgaataa atgatacaat cgttcgactt ctttttttgt
    12121 tgcacaataa atgattgccg ctccttgtgc tctttccatg aagtgttcaa gttcttgttc
    12181 tttttgctgc gttttccgaa caaaaagcga gatattttgc cgattgactg attgtctgat
    12241 ctcttttggt ggtcgatcaa atagtacatg acgtatatct cttgctacta aatctgttgc
    12301 tgtcgccgtt agtgccagtg tgaccgggtt tcctaaaagc tttcggattt ttcctaactg
    12361 ttgatattct ggacggaaat caactcccca ttgagacacg caatgagcct catctactac
    12421 atataacgca atattttgtt ttttcagatg ctctaaaaga gaaggattag tcaacatttc
    12481 gggactcaaa aacaaaaatt tatattgcga tagatgacgg aggacgtatc ttctttcaga
    12541 ttgcgaaagt aaactattga aagcagctac tcgtttctcc cctcgttttt gtaattgcgt
    12601 cacttgatct tccattagag aaagcagcgg tgacacgatg atgaccagac cttccattaa
    12661 ataccccgtc aattgatagc aaaggctttt tcccgttcct gtaggcaaaa tagcgagagt
    12721 atcttctcca tcaagcaacg cctgaataat ttcttcttgg ccgggacgaa atgaatcaaa
    12781 tccaaaatat ttttttaatt catttattaa cattctgtat cgcttccttt ctcaatagat
    12841 agatttgata caaacgaaat tctccataat ctaattgtcc atctacattc cattcgctaa
    12901 agcgccactc tcgcacggaa tccttatttg gttccaaacg ttcgattgtt tctgcagaga
    12961 gaatccactc gtatggaaaa tcatcaaaaa acaaggccca ctcaataaga tgatctgtta
    13021 ctgttcctct ttttaaatga cggattgcca tgatctggtc aattgtctga ccattcataa
    13081 acatctgctt ggtgatcatc atgctttgat tgaaattttg ctgtaataat gaatcgatca
    13141 aagcgtgcca aagcgaatct ggccgttttt cgatttgtgc taacaaaagg tggatacatc
    13201 tgctttgttg cagataaacc tctgactcat cattgttttt tgccagctga taaggaagca
    13261 gccctgttcg ctcatttcca gataattggt tagctaaaaa atctgctgct tcttctggta
    13321 tctccgaaaa cagctgcacc aaactctcat acgcagtatc aatcaaaatt tcacgtggca
    13381 attggctttc actaagccat ttttttagtt ggaaagtata aaaaggactt gtctctgctg
    13441 gaagatagtc ttgaataccc gttgccagat tagaaattac ttgaacagca aatttaatga
    13501 gccgccaact agtttcccct gttctgccat agcggtcaaa acgcaaacca gtatgttcta
    13561 tttgatgttc ggacaacata tcagcccctg caggcgttag ctttgcttca ttttcattta
    13621 tttcgatcca accttgctgc gcaatttttt gcaaaatttg ataaaactta tcctgcttca
    13681 attctggaaa actgttatgg ataaacaaaa gttcgtgggt aaatccataa ataagtactg
    13741 aagtcgttcg ttttcccaca aggagttggt aaagagaact aatccgcagc ttgtgtcctg
    13801 atgaaaatag cgataaaata aaaacaatca tattattctc cttaagttag gatggttaga
    13861 aatgtcacta ctatgtaaac ttgtacctga acgctgtatt gcatgcggtc tttgtcaaat
    13921 cgaagcgcca gatattttcg attatgatga agaaggaatc gttttatttg cccaagaacc
    13981 agaggctcat caacagttcg tgatgccgga agaagaaaca tatgtaaaaa aagcgtatca
    14041 aaaatgtcca gttcgagcga ttttattaga aaataaaata taaatataaa cgaaacttta
    14101 aaaacgtaca ctaagttctc taaacttgaa aataagccgg gatctgtttc tcttgttccg
    14161 tgaaatcccg acttattttg aaagattcat ttgctatcta tagttaattg actagttttt
    14221 tcacggaccc tataggaccc atttatatta tttcaacttt ttcagccagt tttccaatgt
    14281 attgacgatc acagtttgct ggtctgcgtt agaaatttct gcctctccat ctcctttttg
    14341 ctcaccatag ctgccaaaac ctccatgatt tccgccattg attgatacat actctgttct
    14401 ttccggtaaa tactgcttag cttcttgata agcttcctga ttcaatactt tatcttttgt
    14461 cgccgtgatc gataaaacag gaacagctga ttctgtcaat gttccttttt tgtcgggata
    14521 acttgccatg aagaataccc cttttaaatg tttgtctgaa tgattcttat tgacataacg
    14581 acttgccatt acgccaccta atgaatggcc accgatcaca taatcggaat ttggaaaatc
    14641 ttctttgacc ttatccgctt tgtttccgcc catcactgct agatcaagtg gcattttgac
    14701 aatagcgact gaaaagccgt tttttgctaa atcttgcgcc caaatactat aactttctgg
    14761 ctctacgaga gcaccaggat aaaaaataag taatggagac tttccatgat tctttttctc
    14821 tccttcaaaa aataagtaat cttctttgtt ttttccttct tttaccaaca cttgcgcttc
    14881 tgacgatggt tcgtacgtat tttgctgtat atagtaataa ccggctccac cgagtaatcc
    14941 taaaatgatg actaagccaa ttagtattcg tctaaatatt ttcactttct cccacctttt
    15001 ctatctgagt gacccctttt tcttaatcaa gtatgattat accttaaaaa aataagagag
    15061 aaatgcaaac tttccagcat caaataaaaa aagacggaat atccatttga atatccgccc
    15121 tttttgtatg tttattttta tttagcacat aaacgataca cagcttctgc ataaatttcc
    15181 attgctttat acatactact taattcccat cgttcattcg cctggtgcat aaagtcagga
    15241 gtatcaggga acatcgcacc gaatgccaca cactggttca tcgtacgagc aaaagtagcg
    15301 ccgcctgata caaacggttc agtcatgtct ccagtaatat cgcgatatgt ccccaataga
    15361 tttttcacta actcactgtc tagtggcacg tataatgaat caaggaagtc aaattcttca
    15421 taagtcaacc catatttttc tgctgtttta gttaatttag ctactaaatc atctttcttg
    15481 aatgtaacag ggattctcat atctacaccg atttttgttt gttctggtgt gatttccaaa
    15541 cttgcaaagt tcatcgttag ctcgccggat tgttcatctt ctgttttgcc cacgacagct
    15601 tcaccagttg cattttcttg aactaatttt cctaagaaat tgattgggcc aaaatcaaat
    15661 acttctgaaa gagcaattgc cagacgagaa atagcattca ctccttgtgc tgcatctttc
    15721 gcgtggatac ttttaccaag aacgacgatg ccttcacctt gttcttcgaa atcgaattcg
    15781 tgttttttca atgcttcttt tacttcagaa agcttttctc cagagtaagg agctgcatca
    15841 ggaacaacat tcaacgctcc tccagctttt acagaaaact cgttcgttcc tggtcctgtc
    15901 aagtaagctt gcaataagcc tttttctgca taaattaaag gaaattcagc atctggcgca
    15961 aatccttgcg taattccttc ttctttttcg ttatattttt ctaaacaacg ccataaattt
    16021 tcttcatctg taccaaagat aaaacgaata cgtgtcttga attccacgcc agcatcaatc
    16081 aaagctttga cggcatacat tgccgcaata gaaggacctt tgtcatcttg ggatccgcga
    16141 ccaactaacc agccgtcttt tatagttgga tcgaatggtt ttgtttccca attattttcg
    16201 tcccctgctg gaacaacgtc catatgacac aagataccga ataattcatc tccagagcca
    16261 atctctgaat aaccgtaata gccttctgga tctttaaatg ttctaaatcc tagcttttca
    16321 ctgatttcca atactttatc caaagcttca atcacttttt taccgaaggg atgtccttgt
    16381 cctgaatctg cttcatctag tacggaagga attcggatca attcgctcaa tgattctaat
    16441 gcttgttcat gatgttgttc tgtcacaaat tttttcatga tattatcctc ctaatatgtt
    16501 aaaaagggtc atgtcaaatt cctccgacac aacctcttct tctcaaataa tgacgtttta
    16561 agcctgctcc atcagcaagc agaatgttct taacaaatga tgaaagaaaa aacactcact
    16621 gaatggagct attgacttgt ccacgtgctt atcgatagcc gtttatcggt ttcttcattc
    16681 ttatgataga ccttgtacct gtatttgtct aggtttagaa cattgctgct acacctagga
    16741 tcacacagat ggctacaaat aatatcgcaa ttaatttacc ggtaaatttc caccacactg
    16801 aaacatcgaa acgagcaatt gctacagcac ccattacgat agcagatgtt ggtgtaatta
    16861 agtttactaa accagatgca gattgataag cagtgattac taaatcctta ccgacgccgg
    16921 caaattcacc cataggtccc atgattccca ttgttgcagc agctaagccg gaagttgaag
    16981 gaatcaagaa tgacataggg atatagaaaa taaatgtaat aatgatgaat acacttgaag
    17041 ataatgaact taatccttgt tcgccccaat gtaagatggt gtctgtgatt aatccattgt
    17101 tcataaccac ttggatacca cgagcaacag ctactacgat tgctacaccg atcaaatcag
    17161 aagcaccagc taaaaattca gagacaaatt cagactcttt catgccataa gtgattgcta
    17221 ctacgatgga cattactaag aataacatag taatttctgg gaagtaccag tcacctaatg
    17281 gaagcatgct tttacctaga accgtaccta gtaccggaag gtttgttaac cagtttgtca
    17341 atgattcaaa aatcgtgaaa ttcgcattca aggttgtcca aggaatcaaa ctgatgatca
    17401 tgataccaaa agtaataaag aacaatacat taacatgttt ttgttttttc gtgattactt
    17461 cttgacgacc gattgcttca atatcgaaat gcttcatatc ttctttacgt tggttgtata
    17521 ctaatgattt tgttggatct ttttcgatct ttgatgcata acgatacaca tatacaatag
    17581 aaatagctaa caaaataacg aacatcaaga atcgaagacc aataccttca cccatcgaaa
    17641 tcccaacagc ttgtgaagct acgcctgttg caaaaggatt taccgttgaa gctagacagc
    17701 ctacttgaga ccctactaat acgattgcta cagctactac tgtatcaaag cctacagcca
    17761 tcatgactgg gatcagtaaa gcatagaatg gaatcgtttc ttctgccatt ccgtaagtag
    17821 agccgcctaa tgcaaataac agcatcaata ctggaataag cattttttct tttcctttga
    17881 atttcttcac aactgaagca atcccggcat ctagtgcccc tgttttgttg ataataccta
    17941 aaaatccacc aacaaccaaa atgaagaaag agattgggat tgcagcaggt gtttctttag
    18001 ttagctcccc cgctggtaca cctagcatcc cattgattgg cgccatgaaa atatcccaca
    18061 gcccttgagg attttgttgt actcgttcat atgatccagc gacgatgttg cctgcatcgt
    18121 ctacactata gtgcccagct ggtataaacc aagtaagtac agcgattact gcaataataa
    18181 taaataagac cgtatacgct gaaggcattt gaaatttttt cttttttgtt tgttccatct
    18241 tcttcttcct ttcttttccg ttagagacaa caattttggc tctaatgtta ttttatcaaa
    18301 aagagtatca gaacaaaaat aataacacgc attttgtaaa acttttgttc aaaaagttcg
    18361 tttttcccta ttccctcctc ttttttcagt tttttaaagc tttcttcttt ttcattgtag
    18421 cgtttccttt tttttataaa caaacatttt cgtcttttaa aaggagaaga atttcacatt
    18481 tgcaaagaat tgattgattt ttatgcataa atcaaaaaaa tactcaaaac aaacaaataa
    18541 aacgtttaca ttattgcatg tcttgtattt ttttccaaaa aaaaagacca agaaatttct
    18601 tgatcttttt gatatttatt tgactgctgt atgacgttgt tcgaactgat gttgttttcg
    18661 acttaaccat ggaagaagtt gcgcatgcag cactaaaaag acagcactca cgatgaatcc
    18721 tttgatcaag ttgaaaggaa cgatcccaat cagtacatag tttgctaaag acatgcccag
    18781 catctgtgtt gcgttcagcc ctaaaaacat taagtaaaga ggcgtaataa cgaaataatt
    18841 tgcgatactc ataaaaatag ttagagcgat ggttcctgta atgacaccta aaaatttgtt
    18901 tcttccttta tgctgtttct ttacactaaa gaaataataa atcgggaatg taaagatcgt
    18961 tgttgataag aaactagcag tgtctcctac caaattatcc ggagaaaaac ctgtagtcag
    19021 cagatgtaat actgaacgca agaacgctgt tgcaactccg gccgctggac cgaataagaa
    19081 catgctgatc aataccgaga tatcactaaa atcgatcttc aaaaaactaa aagccggcat
    19141 gatcggaaaa gcaaaatact gtaaaactaa tgcgatcgca gcaaacatcg ctactgcaac
    19201 cattttctgt acacgggtgt tcttcatttg aaatcctcct gttaggactc ctcttcagcg
    19261 aactttttga cgtttctctc caaatgaaaa aactctattt tccagggaaa atagagttag
    19321 agttatttga tcaagcgtcg tcaaataagc tctatcttct ttatccagac tatactgtcg
    19381 gtatcggaat ttcaccgatt cagctatttt tttgcaaaat aggtcgtgga ctataaccac
    19441 cggtcgggaa tttcaccctg cccctgaaga cgaaccttat ataatttttg tttacatatt
    19501 cattatacat atctattcaa aaaacgcaac atctaacaat tataaaaagt aagtgttcat
    19561 tttgagtggt tcagcaaact gctgacttct tttttgttca gttcacggta atccccagga
    19621 cgtaggcctt gtaaagtcag atctccgtat ttttcacgtt ttagtttttg cacaggaaga
    19681 ccaactgctt gaagcatatt tttcacttga tgattacgtc cttcgtggat cgtcaattcc
    19741 acgatactgt ggtttgtctt aggatccact gataaaattt ggaatttagc tggagatgtt
    19801 tttcgtcctt cgatctttat tccttttgtc aaaggtgcaa gcatcgattt tgtagcgatc
    19861 ccttttacct ttgcaacgta aactttatcc acttcatgtt ttggatgtgt caggcgctga
    19921 gcaaaatctc catcatttgt cagtagcaag atccccgaag tatcgtaatc cagtcgacct
    19981 acaggataga tacgttcttt taccatcggc aaaagatccg taacaacttt tcttcctttg
    20041 tcatctgcaa cagcagaaat aactccttta ggtttgtaca ataaatagta accgtattct
    20101 tcttgataaa ttgggacttc atccacttcc acagtgtcat gtttcccaac ttttatccct
    20161 aattccctta caacttgtcc gtttactttt acacgtcctg caaggatgaa ttcttctgct
    20221 tttcgacgag aagtgatgcc agcgtgggca attactttct gcaatctctc cattacaatt
    20281 attctccttt attcattcca tcaaagaata gatctggtga cacgtcttct tcaagtgaaa
    20341 tttccatttc ttgtgtatca ggcagttctt ctaatgattt caaaccaaaa taatctaaga
    20401 agtagccagt tgttccatat aatatcggcc gacctggtcc ttctactcta cctttttctt
    20461 caatcagttt atgggcagct agacgctgta ccgctcctga aacctgaact cctcgaatct
    20521 gctcgatttc agctcttgtg attggctgtt tataagcgat gattgctaag gtttcgacgg
    20581 cggcttgaga taatgtgttt gatgtccctt gcgcatatcg cttaagtaag ggagccagct
    20641 cttttttagt cgttaataca taatggcttt ccgtttcaaa aatatgcaaa gctgagtgcg
    20701 agttttgagt gtaagattct tttaattctt tcactaaact tgtgacttct gcttcgctga
    20761 gatttaacag tccgcttaat tcttcattac tgatcccttc ctcgccaaca ataaaaagaa
    20821 gggcttcaag ttcacttaat ttcgtcatgt tcggtatctc ctactgctgg ataaagcata
    20881 attgattcat agttttcttt ttgttgcgca atgatctgcc cttttttcat caattctagc
    20941 aaagccataa aagtagtgac aacttcactt ttagaaggtg actcaaaaaa agaatcgaaa
    21001 gtacagccat gcgcatttct ttttatacgc tcttcgataa ataccattcg ttcttcgatc
    21061 gttgtgtcat cgggcgtgat cgttttttcc actttttgag aaaatttctt acgttctaag
    21121 attttctgca tggcacggat caaatcgatt tttttgactt gtcctcgctt caacggcttt
    21181 tcagcatccg ctaacccatc gatgttcata ggttcttttg tgtaataaag acttcgttcc
    21241 tctgcttttt ctgtcagtct ttcagctgca tatttatatt tacggtattc taaaagctga
    21301 gtgaccaatt cttctcgagg atcttcttcc cataattctt catcgtctgt gccgaattct
    21361 tggacaggaa gcaatgtctt gcttttgatt gccatcaatg tagcagccat cactaagtat
    21421 tctcctgcaa cagctagctc gagtgttttc attgtatgga tataattcat atattgctcc
    21481 gtgatcgtag cgatcggtat atcatatata tcgatttcca tttgctgaat cagatgaagc
    21541 agcagatcga gaggaccctc gaatacatct aatttaatat tgatctccgt caactcattc
    21601 actctttctt ctttttcttt gctagccatt ttccaatgta aggctctgtg atttcagttg
    21661 cacggatgga gacatccggg tacttttcct gtaattcatc taacatttga aagcccatat
    21721 tctcaccgcg ataagacgga ttcaaagaaa tgtcatggat gatcagctga tcgttcgttg
    21781 cccagtaaat accaataatc ccttgaatgt tggttgaacc ttctggcgta aagaaaaaaa
    21841 gttcataatc gtcttcagat tcgtacgtat caatctcctt caacaaagaa gttttacctg
    21901 tcagtttctt atggaaactc aatagtccca tagcaatttt acggttttct ttccggtaat
    21961 gtgttaacat cttctcacct tcctatgact aagcccttgg aaaatgctgc ttataaacat
    22021 ccgtcattct tttcttcgtg atatgtgtat aaatctgagt cgtagagata tccgcgtgac
    22081 ctaacaattc ctgtactgtc cgcaaatcag ctccattttc tagcagatgt gtggcaaagc
    22141 tatgccgcag cgtatgtggt gttacttctt tgtaaatccc agcctctcgt actaactgct
    22201 tcagattttt ccagattcct tgccgagaaa gacctttccc atgattattg acaaatacat
    22261 gcatttcaga tgcgtccttt acaagtaatg gacgtgcttc gtctaagtag cgttccagcc
    22321 attggatcgc ataatcacct aaaggaatga tccgctcttt atctcctttt ccaactgtct
    22381 gtaccagtcc aagagaaaga tgaagatctc ccaatttcaa tccaatcaat tcactgacgc
    22441 gcatcccagt agcatacatg acttccaaga ttgcacgatc tcttatccct aacgtttttg
    22501 tcgtatcagg tgcttcaatc agcttttcta cttctgtcag agtcaatgta cttggtaatt
    22561 tctgtgcttt cttcggcgta tcgatatgtt gcatcggatc atgatcagtg atacgctctt
    22621 gccgtaaaaa ctgatggaat cttctgagac ttgagatcat ccgagtaaca gttgctgtcg
    22681 cttttttttc ttcatgcaac ttctccagaa aacggatgac taaaaagcga tcgacatcct
    22741 gccatgaagc aattttctgc tcctgtagaa aatgcaagta ctgttccaaa tcacgttcat
    22801 agctttgtcg tgtattggcg gacaatcccc gttcaatatt caagtagtgg agataatcgt
    22861 tgatttgctc tttcataata atccccctca tgcctctcta tcatatcaga taccgcttca
    22921 ttctgatagt ctttttccat aatctgagca aagaaaaagc cgtctagaca ctcagacgga
    22981 ctggtttctt ttttcggctc tataatttat gggactgatg aatcagaatt gattcttcag
    23041 ccccattttc attttaggta ttaaaaa
//
LOCUS       DARBEC010000043        22900 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.043, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000043 DARBEC010000000
VERSION     DARBEC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22900)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22900)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22900
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.043"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            29..583
                     /locus_tag="SCL80_001528"
     CDS             29..583
                     /locus_tag="SCL80_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1107400.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADIAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGV"
     gene            complement(756..1229)
                     /locus_tag="SCL80_001529"
     CDS             complement(756..1229)
                     /locus_tag="SCL80_001529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1107401.1"
                     /translation="MRIKPIKDGEQLLFSSEENKPLYLDYVNHNLKIIIKENNLKHIT
                     LHGFRHTHCSLLFESGASVKEVYVRLGHTDIKTTMDDIYTHIAKKQTEETAIRFADFM
                     SSTEEKQGSLQKVKSMVKNEKSRLSLLDSLLNVELSVFFLFRSVCVFETVLILFF"
     gene            1241..1777
                     /locus_tag="SCL80_001530"
     CDS             1241..1777
                     /locus_tag="SCL80_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1107402.1"
                     /translation="MPARIHHISIVNRFIRPTFDFYHNILGLKLLMKTINQDDHTMYH
                     LFFSDNHHRVGTELTFFEVQEGNNQFFGTNTIERTILKVPSEASLHFWEIYFETQGVC
                     HYGIESFSGRPILRFEGPDATRLALVPLREFEDIDDYFPYVTDQIPTEHAILGIDAIQ
                     LRVQYSDATERELLSVGW"
     gene            1817..2188
                     /locus_tag="SCL80_001531"
     CDS             1817..2188
                     /locus_tag="SCL80_001531"
                     /inference="COORDINATES: protein motif:HMM:NF013098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1107403.1"
                     /translation="MTILENQDTQFYQEVQIIQDRENPVAIEGIGSVHHVAFGVENKS
                     DLQRIDKQLQERNFINSGIKDREFFVSLYYRDPNQLLIEIATGEGNLDAKAYENQSPR
                     FEEIPLFLPQYLNFIREQVEQ"
     gene            2472..3743
                     /locus_tag="SCL80_001532"
     CDS             2472..3743
                     /locus_tag="SCL80_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEG1107404.1"
                     /translation="MKLSTQEKQLKNVSLKIPKNKISIFTSVSGSGKSSIVFDTITQE
                     AGRQLNDTYDSFTRLFLPKYSRPEVDEIDNLSTAIVVNQKKIGGSARSTLGTITNINA
                     LFRVLFSCFATPSLGYANAYSFNDPSGMCSVCQGIGKTIAFLIGLLYFSAIDNADFVF
                     SDPFTAEVLSGIEEQAKNHGYFVLVHNVNSAEDVKTIQRNWRFEGFIVVGVRVKEFLE
                     LNNVFDGPVVYIDTHLSKETLRELEQQMNRLFVNTNDYQASTLAVEHLIHYGHEKIAF
                     LSFDYIVGEPSVIQERHQAYIDTLKKYHLTFKSHWIYNNSEFERIYQELAEFTAIVVT
                     GDYLAAKLVKFLKNKNKRVMEQLSIIGFDDITFAELMDPALTTIRLNPGNKGAIAFHQ
                     LIGLAKQEEIKNQVLLLEGELISRDSVHKIK"
     gene            3988..5013
                     /locus_tag="SCL80_001533"
     CDS             3988..5013
                     /locus_tag="SCL80_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107405.1"
                     /translation="MVGIRDVAKRAGVSIATVSRVLNEDPTLSVTDETKRKIAESVNF
                     YHYVKKSPVAKNLTNIALITTVSEVDELEDPYFRTIRRGIQVEAEQSKVNLKKIIRLS
                     ETPLEQDDLLKCQGVLIIGQVLSSVIEEVSAINSNVVVIDDPSIEAEVDAVYTDLTKA
                     TNQHLDRLYKKGHRNIAFIGGGRVRLDSQGNKHVSDDDYRKIAYETWMKQKGLEKYSQ
                     IYLEGWTTLDGMQAADLLLKKHAKDKLPTAIMVGNDPIAVGVYRSLQKNGLHIPEDIS
                     IVSFDNIEVAEFLTPALNTVNVNTEEIGKLAVRMVNERIKEVRNVSIKVIVSNNIIVR
                     ESEKNID"
     gene            5307..6416
                     /locus_tag="SCL80_001534"
     CDS             5307..6416
                     /locus_tag="SCL80_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEG1107406.1"
                     /translation="MLDEFEKQNPDIKVEMETTPWDQYWVKLEAATTGGNMPDVVTMH
                     SSESYKYMSQNALMGLDDVIKENDIDMGNFTEGIADFYTLEDELYAIPKDASVVGLWY
                     NKELFDNAGVDYPDETWTWETLRQAAIDLTDESKGIYGFASHIGTETGYWPFIYQNGG
                     EVFADDNTKSGLNTPEAQEALAFYRDIALVDHASPTITELQDPDKLSRFQSGRLAMMI
                     EGNWFAPGFLKNDYLVENVASAVLPKGKVNATITNGLGWAASASTKYPEEVKKLMAFL
                     ASEDANKIQAETGASIPALKGFEDEWAASSDTIDLSAFGKMLEYGVPRPYNKDGLRAE
                     QAEVQIINRILSGEVTVEDGTAQIEKEVNAILSGN"
     gene            6499..7395
                     /locus_tag="SCL80_001535"
                     /pseudo
     CDS             6499..7395
                     /locus_tag="SCL80_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017648409.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
     gene            7411..8235
                     /locus_tag="SCL80_001536"
     CDS             7411..8235
                     /locus_tag="SCL80_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107407.1"
                     /translation="MKNKFFSNAPIHIMLILGGIAMALPFIWMILTSGKTLVESTQIP
                     PTIFPEKFQLSNYQQVLDLLPFMAFYINTFLMVVGRVIGSVLFSAMAAYACARLSFPG
                     KNILFSLVLIQMMIPGSIFITPQYLLAQKLGILNTIPALILPGVVSAFGTFLLRQFFL
                     GLPDELEEAAYLEGANIWTIFFKIMLPLAKSGLVALGIFTALFAFKDLMWPLIVNTSI
                     DKMPLAPGLANLQGQYATNFPQLMAGSLLAIWPIVVIFIVFQRKFIEGIATTGSKL"
     gene            8267..9577
                     /locus_tag="SCL80_001537"
     CDS             8267..9577
                     /locus_tag="SCL80_001537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005235059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase/alpha-galactosidase"
                     /protein_id="HEG1107408.1"
                     /translation="MTKITFIGAGSTIFAKNVLGDAMLTPSLQDAHIALYDIDEKRLK
                     ESNLMLKNINKNSNENRADIVSYYDRKEALREADFVINAIQVGGYKPSTVIDFDIPKK
                     YGLRQTIGDTMGIGGIFRTLRTLPVMLDFAKEMEEVCPNAWLLNYTNPMAMLTMGVLK
                     ATSIKTVGLCHSVQVCVPELFEHLGIADQYDLNDFQWKIAGINHMAFLLEITKNGEDF
                     YPTIRQLAAEKPNPHPDSVRFELLKRFGYYITESSEHNAEYHPYFIKSKYPELIDELG
                     IPLDEYLRRCEQQINDWESMREDIVNNGELTHTRSREYASYIMDSITTGTPSVIAGNV
                     LNKGLITNLPTDVCVEVPCLVDKNGVQPTYVGELPTQLAALNRTNINVQELTVEAALT
                     LDKSKIYQAAYLDPHLSSELSLTDIKNLVDDLIEAHGDYLPEYN"
     gene            9725..10984
                     /locus_tag="SCL80_001538"
     CDS             9725..10984
                     /locus_tag="SCL80_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1107409.1"
                     /translation="MKWSKKIILACIALGATISLTACGKNQEDASGKVEIEFFNQKKE
                     MTQTIQEIAKDFEAKNPDIHVKVVDVPNAGEVIKTRMLAGDVPDVINLYPQSIELKEW
                     AKAGYLEDLTEEPYLENIKNGYAQRFAIEDRVYSIPLTANVYGFYYNKTAFEEMGIQA
                     PETWAEFEKIVAEIKDQNKVPFAIAGAEGWTLNGYHQLAIATAAGGEEEANNVWRFSE
                     VNGINAESKEMQKDFNRLDLLREPKALQNNWQGAGYNDTVVTFTKGDALIMPNGSWAM
                     PMIHSQNPDFEVGTFPFPADEAGQSLTIGAGDLALSVSATSQHKEEAKRFVEYMTTPE
                     AMQKYYDVDGAPCAVEGVIENTQDSPLSGLTELAFTDRHLVWLAKDWNSENDFYTLTT
                     NYLHNGNQKAMINALNAFFNPMKADGR"
     gene            10994..11866
                     /locus_tag="SCL80_001539"
     CDS             10994..11866
                     /locus_tag="SCL80_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018363968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107410.1"
                     /translation="MKIKEILNRYWAIIFVAIPIILQMIFFYFPMVQGAFYSLTNWTG
                     LTYNFDFVGLNNYKILFSDPKFIKSIGFTLIVTLCMIAGQIGFGILIARALNANIKGK
                     TFFRAWFFFPAVLSGVVVSLVFKQIFNYGLPKIGSMLGIEFLQTSLLGTTWGAIFAVI
                     FVLLWQGIAMPVIIFLAGFQSIPQEIIEAARMDGANDKQIFWNIEITYLLPSISMVFI
                     MALKGGLTAFDQIFALTGGGPSDATTSLGLLVYNYAFKNNQFGYANAIALVLFILIGI
                     VSLAQLKLSKKFEI"
     gene            11878..12708
                     /locus_tag="SCL80_001540"
     CDS             11878..12708
                     /locus_tag="SCL80_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000711263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107411.1"
                     /translation="MKTSKEKNISKYLLLLLGGFMILVPLVSTLFGSFKTTPDIMNNF
                     FRFPNPATLENFRRLLADGIGHYFWNSAIITIIAVVAVTAIIPMAAYSLARKMTRGKA
                     YGLMYLFLILGIFVPFQVIMIPITVMMSKIGLANMWGLILLYLTYAVPQTLFLYVGFI
                     KLNVPISLDEAAEIDGASHFTTYFKIVFPMLKPMHATTIILNALWFWNDFMLPLLILN
                     RDSNMWTLPLFQYNYTGQYFNDYGPSFASYTVGIVTITLVYLAFQRSIIAGMSNGAVK
                     "
     gene            12748..14931
                     /locus_tag="SCL80_001541"
     CDS             12748..14931
                     /locus_tag="SCL80_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEG1107412.1"
                     /translation="MIIFDNHTQTFHLQNKKISYLLGIEENNYLTHLYFGKKITHYSG
                     GYRYPRTDRSFSPNNANAQDRLLSLDTLPLEFPGYGHGDFREPAHNIKLGNGSRVNDF
                     RYDSYEIQKGKPSLAGLPALYTEDDDEAETLIIQLKDAISGLTLQLFYTIYQNHSVII
                     RSTQLVNNSDETVEINRLASQSIDFPNRPLELIHLNGTWARERQMTREKIHTGIKVLD
                     SKRGSSSHQQNPFVALVDAKTDEFQGEAYGFNLVYSGNHETVIQKDPFDQTRVIMGIN
                     SFNFGWQLKTGDSFQTPEVVMVYSNEGLNTLSQTYHELYNHRLIRGNYRLKERPTLIN
                     NWEATYFDFNEEKIMNIVDDAKNLGIEMFVLDDGWFGGRENDFTSLGDWVETEGKLAC
                     GLENLAKQVHDNGMKFGIWFEPEMISEDSELFRAHPDWALGIPGRDRSISRNQYVLDF
                     SRQDVRDNIYQQMTAIFDRVPIDYVKWDMNRNMTEVYSVLLDSTMQGEVAHRYMLGLY
                     EFLEKLTTNYPTILFESCSGGGGRFDAGFLYYMPQTWTSDNTDAVARLKIQYGTSLVY
                     PISSMGAHVSAVPNHQTGRETSLTIRGNAAMAGVFGYELDLTQLTSEEKAEIKEQVAF
                     YKKHRQLLQYGTFYRLESPFEGNDTAWMFVSKDQTEAMVFYFRVLAEAAHPLVTLKLA
                     GLAEEATYQMEDVTFAGDELMNLGFYIDSELHGDYATQRFYLKQI"
     gene            14992..16212
                     /locus_tag="SCL80_001542"
     CDS             14992..16212
                     /locus_tag="SCL80_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligo-1,6-glucosidase"
                     /protein_id="HEG1107413.1"
                     /translation="MFYLHAFTKKQPDLNWENEEVREEIYAMINYWLDKGLGGFRIDA
                     ILNIKKKLEYGIFESDGEDGLAFIGPWILNQSGIEVWLKEMRERTFKPHNSMTVAEAD
                     VPNERLDEYIGEDGYYSMVFDFSYTDIDVPETGEWFKDSQWTWTDMRTNIFTNQLVTQ
                     DKGWGALYLENHDQPRSINKYIPEEWINDYSKKMLATLFMLLRGTPFIYQGQELGMTN
                     IEMASLEDFDDVATHSQYKRALEYGLAPEQALKAVNKRSRDNSRTPMQWNTQKNARFS
                     TSDNVWLKVNPNYPELNAEAQLTNQQSVFNYYHQLIKLRKNSVYKDVLIYGQFLPVKD
                     KNEHILLYERQLEDKIVLVILNVTATEQEYYVDEQYQQVLINNYEDVLLDQSRKLRLR
                     PFESIVLANYGGQL"
     gene            16209..17669
                     /gene="gtfA"
                     /locus_tag="SCL80_001543"
     CDS             16209..17669
                     /gene="gtfA"
                     /locus_tag="SCL80_001543"
                     /EC_number="2.4.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HEG1107414.1"
                     /translation="MKIKNEAMLITYADSLGKNMGELKQVLDKHLQGVIGGVHLLPFF
                     PSTGDRGFAPSDYTTVDPALGTWEDVEALGEDYYLMFDFMINHISRESKFFQDFKKNH
                     EQSPYKEMFIRIHEFFPENRPTQEDIDLIYKRKDKAPFQTVEFADGTTEEVWNTFGEE
                     QIDLDVTKEVVKEFIRETIKDMANHGCSLIRLDAFAYAIKKLDTNDFFVEPDIWELLD
                     EVRQEAAKYDVELLPEIHEHYSIQMKIANHDYYIYDFALPMVTLYSLYSGKSERLANW
                     LKMSPMKQFTTLDTHDGIGVVDARDLLTDEELDYTSEELYKVGANVKKVYSSANYNNL
                     DIYQINSTYYSALGDNDRSYLLARAIQVFAPGIPQIYYVGLLAGKNDIELLEATKEGR
                     NINRHYYDLAEIEEEVQRPVIQQLFALLKFRNESAAFDLDGTIEVATPSESEIVITRK
                     NKNGSDSATLTANLDTKEFIIESKDIKIFTSVALKF"
     gene            17746..18852
                     /locus_tag="SCL80_001544"
                     /pseudo
     CDS             17746..18852
                     /locus_tag="SCL80_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175341.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
     gene            19174..19383
                     /locus_tag="SCL80_001545"
     CDS             19174..19383
                     /locus_tag="SCL80_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1107415.1"
                     /translation="MVVITKNELMKLGYGKYQSERIIRQAKAIMVSKSYTYYENRRLG
                     LVPVESVEEVLGMKIEVEEDFQNHG"
     gene            19376..19525
                     /locus_tag="SCL80_001546"
     CDS             19376..19525
                     /locus_tag="SCL80_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HEG1107416.1"
                     /translation="MVNFKKDKNGKYHFTASLGFDKRTGKRVQKNVEGLKQLKKKKSV
                     YRTCF"
     gene            19679..20344
                     /locus_tag="SCL80_001547"
     CDS             19679..20344
                     /locus_tag="SCL80_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1107417.1"
                     /translation="MELREIRPIDIQKWQNNLLSKYKNSYVRNMYGIFQMSLDRAVVL
                     GLLEKNPAKVIGNVKKRKTTVDFWTKEEFTKVISTFYIDDLYQHFGFICMWLLFMTVM
                     RLGEATALTWKDIDFENKNLRVNKTLYYKRAKTYEFVEPETASSIRTIELDDNTITFL
                     KAWKERQVSIGGMSFVLSYNGTPTQKYTIRHIMRRHATIVGVHIIRIHGLRHSHASLL
                     ISQ"
     gene            complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="SCL80_001548"
     CDS             complement(20628..21713)
                     /gene="xerS"
                     /locus_tag="SCL80_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEG1107418.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLDNNPMDQVATITDKTTLAARAN
                     AIEKKLFLDKDTQNYLDYIANEYKNTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VININLDDLSLDKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRIQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHSSTNTTTLYTHIDNAATIDALNAL"
     gene            22047..22280
                     /locus_tag="SCL80_001549"
     CDS             22047..22280
                     /locus_tag="SCL80_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antitoxin"
                     /protein_id="HEG1107419.1"
                     /translation="MAIKERKFREVGNSVVLTISKEFLKENGLNPGDTVFLDEEKLRA
                     AITKKQALSDEIDLAISQALTEYDTTFRELVDK"
     gene            22277..22684
                     /locus_tag="SCL80_001550"
     CDS             22277..22684
                     /locus_tag="SCL80_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1107420.1"
                     /translation="MSIHYLEAKDIIKMNVIQIKKYSPNEPLGVKDANALEMCVGQLR
                     ASAFGEEVYPSIYEKAAILLIQLIKKHPFHNANKRTAFLATFVFLKINGQLLAIDQKD
                     AVNLAVYIATYDKDFDQLKYEVINTIKTHTQPI"
BASE COUNT         7821 a         3435 c         4386 g         7258 t
ORIGIN      
        1 cagaatattt ttaagaaggg aaacaatagt gagctataat gtacaaccgt taaggacaaa
       61 tgaagagata caagattttc agttttggct aagaagaaca agtaatccgg aaagagatag
      121 ttttttattt ttgtttggaa tcaataatgg gttacgtatg tcagatatta taggattaaa
      181 agttggagat atacgaggca aaagtaagcc tattattgtt gaacataaaa caggtaagcg
      241 aaaaccgatt tttatagata atttgagaga agaaatttta ttatatactg aaggtaaaga
      301 agaaaatgat tggttatttc cgagtcggca acaaggtcga catataacaa gggatcgtgt
      361 ctatcagata tatgcagata tagcagaaaa attaggacga gatgatatag gaacacatac
      421 attaagaaaa acttttgggt atcactacta caagaaaact agagacattg ctaccttaat
      481 gtttattttt aatcatagct cgcaagcaat tacaaaaagg tacattggta taactgaaga
      541 tgaaatagga gcatcacttc gtggatttaa actaggtgta taatttaaaa atttttataa
      601 acagtaaaga caaagattaa aagctatttt tatctagaac gattttattt cataaagttc
      661 gcggaataaa agctggggtt actggtactt ggtataggtt cgtgttatat aaactaagta
      721 attaaataac atttttaggt tggaaaagga tgaaattaaa aaaagagaat caatacggtc
      781 tcaaaaacgc aaacgctgcg gaataagaaa aatactgata actcaacgtt tagcagacta
      841 tctaataggg atagtctgct tttttcgttt ttgaccatac ttttgacctt ctgcaaactt
      901 ccttgctttt cctctgtaga actcatgaaa tctgcaaaac gaattgccgt ttcctctgtc
      961 tgctttttcg cgatatgggt gtatatatca tccatagtgg ttttaatatc tgtatgtcca
     1021 agtcgaacat acacttcttt gacagacgca ccagattcaa agagcagact acaatgggta
     1081 tgacgaaaac catgaagtgt gatatgtttt aagttattct ctttaatgat gattttcaaa
     1141 ttgtgattta catagtctaa ataaaggggc ttattttctt ctgatgaaaa caaaagttgt
     1201 tcaccatctt ttataggttt gattctcatt tttagatatt atgcccgcac gtattcatca
     1261 catttctatt gtgaatcgct ttattcgacc gacatttgac ttttatcata atatactcgg
     1321 tttaaaatta ctaatgaaaa caattaatca agacgatcat accatgtatc acctgttttt
     1381 ttcagataat caccaccgtg tgggaactga actgactttt tttgaagtac aagaagggaa
     1441 caaccagttt tttggaacca atacgattga acgaacgata ctaaaagttc cttccgaagc
     1501 atcattgcat ttttgggaaa tatattttga aacacaaggc gtttgccatt atggaatcga
     1561 atcattttct ggacgaccta tcttacgttt tgaagggcca gatgcaacac gattagcttt
     1621 agttccctta cgcgaatttg aagatattga tgattatttc ccgtatgtca cagaccagat
     1681 ccctacagag catgccattt taggaattga tgccattcaa ctacgcgtac aatattccga
     1741 tgcgactgaa cgagagttat tgtcggtagg ctggtagcac aaagaatacg tcagtttctt
     1801 ttcgggtcat caacgagtga caatcttaga aaatcaagac actcagttct atcaagaagt
     1861 tcagattatt caagatcgtg agaatccagt tgccattgaa gggattggta gcgtccatca
     1921 tgttgcgttt ggcgtagaaa ataaatctga tttacaacgc atcgacaaac aattacaaga
     1981 gcgcaatttt ataaattcag gaataaaaga tcgtgaattt tttgtatcac tttattatcg
     2041 tgacccaaat caattattga ttgaaattgc tacaggggaa ggaaacctag atgcaaaagc
     2101 gtatgaaaat caaagtccac gctttgaaga aattccttta tttttaccgc agtacttaaa
     2161 tttcattcgt gaacaagtgg agcaataatt agctaatcaa atccatgacg aagagtaaga
     2221 ggttgactca taagtcaaaa aataaagaaa aacgcatcaa atccattcta aagaatgttg
     2281 atttgatgcg gttcctattt ttataaacta tcattttcga ctaataaaac tacttatgag
     2341 tcagcctctc tttttttctt gatttttata cctctttcta ggtaaactaa acatctgcca
     2401 aaaataccgt gtctcattga aacattctca actatttcaa aataaagggg agtaccaaat
     2461 gaacgagtat attgaaatta tccacgcaag agaaacaact taaaaatgtc agtctcaaaa
     2521 ttcccaaaaa taaaattagt atttttacca gcgtatctgg ttcaggaaaa tcatcgattg
     2581 tttttgatac tattacccaa gaagctggtc gtcaactgaa tgacacctat gatagtttta
     2641 cccgtttatt tttacctaaa tacagtcgtc cagaggttga tgagattgat aatctttcta
     2701 cggcgattgt ggtcaatcaa aagaagattg gtggcagtgc gcgctcaacg ttagggacga
     2761 ttactaacat caatgcgtta tttcgtgtgt tgttttcatg ttttgcaaca cctagtttag
     2821 gatatgcaaa tgcttattca tttaatgatc ctagtggaat gtgttctgtc tgtcagggaa
     2881 tcggtaagac gattgcattt ttaataggat tgctttattt ttcggctatt gataatgctg
     2941 attttgtatt ttctgatcca tttaccgcag aagttttgtc tggaattgag gaacaggcaa
     3001 agaatcatgg ttattttgta ttggtgcata atgttaattc agcagaagat gttaaaacta
     3061 ttcagagaaa ttggcgtttt gaggggttca ttgttgttgg tgtacgtgta aaagaatttt
     3121 tggaattaaa taacgttttt gatggtcctg ttgtttatat tgatacgcat ctttcaaaag
     3181 agacattaag agaacttgaa caacaaatga atcgtttatt tgtaaataca aatgattacc
     3241 aagctagtac attggcagtg gaacatttga ttcattatgg tcatgagaaa atagcgtttc
     3301 tttcttttga ctacattgta ggtgaaccaa gtgttattca agaacgacat caagcttata
     3361 tcgatacatt aaagaaatat catttaacct ttaaatcgca ttggatatat aacaactctg
     3421 aatttgagcg aatttatcaa gaattagctg aatttactgc aattgttgtt actggagatt
     3481 atttagcggc taaattagta aaatttttaa aaaataagaa taaacgagtg atggaacaat
     3541 tatcgattat tggatttgat gatattactt ttgcagaatt aatggatcct gcgttgacaa
     3601 caatacgact aaatccaggt aacaaggggg cgattgcttt tcaccaactg attggactgg
     3661 ccaaacaaga agaaataaaa aatcaagtac tactattaga gggagaattg atttctcgag
     3721 actctgttca caaaatcaaa taaaaaagct ctgtgaaata agtatagtta acaataaaat
     3781 atccgaacga tagagagaat gaactgtgat ctagcagttc ctcgtcgcaa attcactaga
     3841 attagcatta tttactctaa attgttcgga tattttttct tgtttttaat tatgattaca
     3901 gtgcctattt ttaacgaaat ttgcttattt tcaaaatgta tgattatatg ttagtaaaca
     3961 ctttcaaata tgaaaagagg agctattatg gtaggaatta gggatgttgc aaaacgggcg
     4021 ggtgtgtcca ttgcaacagt atcgcgcgtg ctaaatgagg atccgacact ttcagtaacc
     4081 gatgaaacca aacgaaagat tgcagaatcg gttaattttt atcattatgt caaaaaaagt
     4141 cccgttgcaa aaaatttaac aaatattgca ttaataacga cagtttctga agtagatgag
     4201 ttggaagatc cctattttcg aacgattcgc agagggattc aagtcgaagc agaacaaagc
     4261 aaagtcaatt tgaagaaaat tattcgtttg tcagaaacac cgttggagca agacgatttg
     4321 ctaaaatgtc aaggtgtgtt gattattggg caggtgctat cttctgtgat tgaagaagta
     4381 tcagcaatta attcgaatgt cgtagtgatt gatgacccta gtattgaagc tgaagtagat
     4441 gcagtttata ctgatttaac aaaagcaacg aatcagcatt tagatcgatt gtataaaaaa
     4501 ggacaccgaa acattgcgtt tatcggagga ggacgtgttc gtttagatag ccaaggaaat
     4561 aaacacgtgt cagatgatga ttatcgaaaa atagcctatg agacgtggat gaaacaaaaa
     4621 ggattagaaa aatatagtca aatttattta gaaggctgga ccacacttga cggaatgcag
     4681 gcagcagatt tacttttgaa aaaacatgct aaagataagt tgccgactgc tatcatggta
     4741 ggaaatgatc ctattgctgt gggggtttat cgttcacttc aaaaaaatgg tctacatatt
     4801 ccagaagata tttcgattgt gagttttgat aatattgaag ttgctgaatt tttaacacct
     4861 gcattaaata cggtgaatgt gaatacagaa gaaattggaa aactagcagt tcgcatggtt
     4921 aatgaacgaa taaaagaagt tagaaatgtt tcgatcaaag tgattgtttc taataatatt
     4981 attgttagag aaagtgaaaa aaatatcgat taaaaaagta aacatccaaa aaagggtgtt
     5041 tacttttttg tttactaata tttttagtaa aaaatctcga caaaacatta agtaaacagt
     5101 tatggtgttg ttaacaaaaa gaaaacgcat tcaaaacagg aggaattaag atgaaacatt
     5161 tcaaaaaaat agctttaaca gggatagctg ttttaactgg gacattactc gcagcatgtg
     5221 gtgggggaga tagtaattct aaagacgggc aagtaacttt aagctatgcg agttgagata
     5281 ctgaccaagc tccaggacta agaaaaatgt tagatgaatt tgaaaaacaa aatcctgata
     5341 tcaaagtaga aatggagaca acgccttggg atcaatattg ggttaaatta gaagctgcaa
     5401 caacgggagg taatatgcca gatgtagtaa ccatgcactc tagcgaatcc tataaatata
     5461 tgtcacagaa tgctttgatg ggacttgatg atgttatcaa agaaaatgat attgacatgg
     5521 gaaactttac tgaaggaatt gctgatttct atacgcttga agacgaacta tacgcaatcc
     5581 ctaaagatgc ttctgtagtg gggctatggt acaacaaaga attatttgat aatgcaggtg
     5641 tcgattatcc agatgaaaca tggacatggg aaacactcag acaagctgca attgatttaa
     5701 cagatgaatc aaaaggcatc tatggttttg catcacatat tggaacagag actggctact
     5761 ggccatttat ttatcaaaat gggggagaag tatttgccga tgacaataca aaatcaggtt
     5821 tgaatacccc agaagcacaa gaagctttag ctttttatcg agatattgca cttgttgatc
     5881 atgcttcacc aacaattaca gaattacaag atcctgataa gttaagtcgt tttcaatcag
     5941 gtagattagc aatgatgatt gaaggaaact ggtttgcacc gggatttttg aaaaatgact
     6001 atctagttga aaatgttgct tctgccgtgt tacctaaggg aaaagttaat gcaacaatta
     6061 caaatggact tggttgggct gccagtgcga gtacaaaata cccagaagaa gtgaaaaaac
     6121 taatggcatt tttagctagt gaggacgcca ataaaatcca agcggaaacg ggggcatcaa
     6181 tcccagcact aaaaggcttt gaagacgagt gggctgcttc ttcagataca attgatttga
     6241 gtgcttttgg aaaaatgttg gagtatggag tccctcgtcc atacaataaa gatggattac
     6301 gagctgaaca agcagaagtt caaattatta accgtattct gtctggtgaa gtaactgttg
     6361 aagatggaac cgctcaaatt gaaaaagaag tcaatgcgat attgagcgga aattaaatta
     6421 gttaattata caatatacag gcgaaaggat tctatttcgt ctgtatatta atggatgaat
     6481 accaaaaagg gggatattat gaaaaatcga atttttggaa aatatcgtaa agatcaagtg
     6541 atatggggct atatttttat ttttccaacg gcattaggat taattttatt aaatatatgg
     6601 ccagcaattc aaacagcaat tttaagtttt caaaaaacgg tagggttcgg tgatactgaa
     6661 tgggtcggat taagaaatta ccaaaaaatg tttgcagacg gtgaagtttt tcagtcttta
     6721 ttaaacacat taatttatgc agtggtttcg atacctagta ttgttgtatt atccttgttg
     6781 gtggcagttt taatgaatag aaaagttaaa ggattgtcga tttatcggac aatttatttt
     6841 ctaccagtcg ttgctgcacc atccgcagtc gcaatgattt ggcgctggat gttcaataat
     6901 gattatggac tgattaataa catgttggct aaaataggtc tcgatggacc agcatggtta
     6961 acggatccat cgattgcgat ttattctatt atcattgtag gagtttggag tgctattggt
     7021 tataacatgg tgttattact agccggctta taagaaattc ctaaagatta ttatgaagca
     7081 gcggaaattg atggtgctag tccaatccag cagtttttca acattacgtt accattagtt
     7141 acaccaacat tattttttgt agttgtaaca acagttatca atgctttcca agtatttgat
     7201 gttattttta tgatgattac tcctaatagt accgccatgt ttaaatcaca atcattagcc
     7261 tatttattct ataaacattc atttatttta aatgacaagg gatatggttc ggcaatcgtt
     7321 atgttccttt tagtgattat attaattatt actgcaattc aaatgaagtt gcagaaaaag
     7381 tgggtaaact attaaggagg ctatcaaatt atgaaaaata aatttttttc aaatgcgcca
     7441 atccatatca tgcttatttt aggtggtatt gcaatggcat taccttttat ttggatgatt
     7501 ttaacttcag ggaaaacgtt ggttgaatct acccaaattc cacctacaat tttccctgag
     7561 aaatttcagc taagtaacta tcaacaagta cttgatttat taccattcat ggctttttat
     7621 atcaatacat ttttaatggt tgttggacga gtgattggct cggttttatt ttcagcaatg
     7681 gctgcatatg cttgtgcgcg tctaagtttt ccagggaaaa atatcctttt cagcttagta
     7741 cttattcaaa tgatgattcc cggttccatt tttattactc cacagtattt attagcacaa
     7801 aaattgggta ttttaaatac aattccagca ttaatattac caggggtagt tagtgcgttt
     7861 ggtacgtttt tattaagaca atttttctta ggtttacctg atgaattgga ggaagcggcc
     7921 tatttagaag gtgccaatat ttggacgatc tttttcaaaa ttatgttgcc gcttgctaaa
     7981 tcaggattgg ttgcacttgg aatattcaca gcgttgtttg catttaaaga tttaatgtgg
     8041 ccattgattg tcaatacttc aattgataag atgcctttag ctccgggatt agcaaactta
     8101 caaggacaat atgccacgaa cttcccacaa ttaatggcag gttcgttatt agcaatttgg
     8161 ccaatagttg tgatattcat cgttttccaa agaaaattca ttgaaggaat tgctacaaca
     8221 ggtagtaaat tataaaacta ttaattagtg attggagagt aagtacatga caaaaattac
     8281 atttattggt gcaggaagta ctatttttgc aaaaaacgta ttaggcgatg cgatgttaac
     8341 tccaagtcta caagatgcac atattgcatt gtacgatatt gatgaaaaac gactaaaaga
     8401 atcaaattta atgcttaaaa atattaataa aaattcgaat gaaaatcgtg ctgatattgt
     8461 ttcttattat gatcgcaaag aagcgttaag agaggctgat tttgttatca atgctattca
     8521 agtgggtggt tataaacctt caacagtgat tgattttgat attcctaaaa aatatgggtt
     8581 gcgacaaact attggtgaca ccatggggat tggtgggatt tttagaaccc tacggacact
     8641 accagtaatg ttagactttg ctaaagaaat ggaagaagtt tgtccaaatg cttggttatt
     8701 gaactataca aatccgatgg cgatgttaac catgggtgtt ttaaaagcga cgagtattaa
     8761 gactgttggt ttatgtcata gcgttcaagt atgcgtacca gaattgtttg agcatttagg
     8821 aatagccgat caatatgact taaatgactt tcaatggaaa attgcaggca ttaatcatat
     8881 ggcattcttg ttggaaatta caaaaaatgg ggaagatttc tatccaacaa ttcgtcagtt
     8941 agctgcagaa aaaccaaatc cacatccaga ttctgtacgt tttgaattgt tgaaacgttt
     9001 tggttactat attacagaat caagtgaaca caatgcagaa taccatccat actttattaa
     9061 atcaaaatac ccagaattga ttgacgaatt agggattccg ttggatgaat acttacgtcg
     9121 ttgtgaacaa caaattaatg attgggaaag catgcgagag gacattgtaa ataatgggga
     9181 gttaacacat acgagaagcc gtgaatatgc ttcatatatc atggattcta ttacgacagg
     9241 aactccgtca gtgattgcag gaaatgtctt aaataaaggc ttaatcacaa acttaccgac
     9301 agatgtttgt gtggaagtac cttgtttagt cgataaaaat ggcgtgcaac caacctatgt
     9361 cggtgaattg ccaacacaat tagctgcttt aaatcggacg aatatcaatg ttcaagaact
     9421 gacagtagaa gccgctttaa cactagataa atcaaaaatt tatcaagcag cgtatttaga
     9481 tccacatcta tcatctgaat tgtctttaac agatatcaaa aatttagtgg atgatttaat
     9541 cgaagcacat ggggactatt tacctgaata taattaaaaa gaagctgaaa gagtaaggaa
     9601 ttttcaattc cttactcttt tcttagttaa atttagtaaa aatattgaca aaacacttag
     9661 taaacagtta cattagtgtt aacaaaaaga aaacgcattc aaattgtata aggaggagca
     9721 taaaatgaag tggtctaaaa aaattatttt agcttgtata gcactaggag caacgatctc
     9781 tttgactgct tgtggaaaaa atcaggaaga tgcaagcggt aaggtagaaa tagagttttt
     9841 taatcaaaaa aaagaaatga ctcaaacgat tcaggagatt gctaaagatt ttgaagcaaa
     9901 aaaccctgat attcatgtaa aagttgtgga tgtaccgaat gctggtgaag taattaaaac
     9961 acgtatgctt gcaggcgatg taccagatgt cattaatctt tatcctcaaa gtatagaatt
    10021 gaaagaatgg gcgaaagctg gttatttaga ggacttaacc gaagaaccgt atttagagaa
    10081 tatcaaaaat ggatatgcac aacgttttgc tattgaggat cgtgtatata gcataccatt
    10141 aaccgctaat gtctatggtt tttactataa caaaactgct tttgaggaaa tggggattca
    10201 agcaccagaa acttgggcag aatttgaaaa aatagtagct gagattaaag atcaaaacaa
    10261 agtaccgttt gcgattgcag gagcagaagg ctggacttta aatggttatc accaactagc
    10321 tatagcgact gctgctggtg gtgaagagga agcgaacaat gtttggcgtt tctcagaagt
    10381 taatggaatt aatgctgaaa gcaaagaaat gcaaaaagat tttaatcgat tagatttgtt
    10441 aagagaacca aaagctttgc aaaacaactg gcaaggtgcc ggttataacg atacagtagt
    10501 tacttttaca aaaggtgatg cgttgattat gccgaatggt tcatgggcaa tgccgatgat
    10561 tcattcacaa aatccagatt ttgaagtagg aacttttcct tttccagcag atgaagcagg
    10621 tcaaagtcta acgattggcg caggagacct agccttatca gtttctgcaa cttctcaaca
    10681 taaagaagaa gccaaacgtt ttgtagaata tatgacaaca cctgaagcga tgcagaaata
    10741 ttatgatgta gatggtgcac cgtgtgctgt tgagggcgtt atcgagaata ctcaggattc
    10801 accattaagt ggtttaactg aacttgcttt tactgatcgt catttagttt ggttagccaa
    10861 agattggaat agtgaaaatg atttttatac cttaacaaca aattatttac acaatggcaa
    10921 tcaaaaagcc atgattaacg cattaaatgc tttctttaat ccaatgaaag ctgacggaag
    10981 gtaggaaaat aaaatgaaaa taaaagaaat tttaaatcgt tactgggcaa tcatttttgt
    11041 agcaattcca attattttgc agatgatttt tttctacttt ccaatggttc aaggtgcttt
    11101 ttacagttta actaattgga ctggtctgac ttataatttt gattttgtgg gtttgaacaa
    11161 ttataagatt cttttttctg atccgaagtt tatcaaatcg attggcttca ctttaattgt
    11221 gacgctttgc atgattgctg gacaaattgg ttttggtatt ttgattgcgc gggcgttgaa
    11281 cgcaaatatt aaagggaaaa cattctttag agcatggttc ttttttccag cggtcttatc
    11341 gggcgtggtt gtttctttag tatttaaaca aatttttaac tatggtttac caaaaattgg
    11401 tagtatgtta ggcattgaat ttttacaaac cagtctttta ggaacaacat ggggagcgat
    11461 ttttgcagta atttttgttt tactttggca aggaattgcc atgccagtca tcattttctt
    11521 agcgggtttt caaagtattc cacaagaaat tattgaagct gctagaatgg atggggcgaa
    11581 tgataagcaa attttttgga atattgaaat cacctactta ttaccaagta tttcaatggt
    11641 atttattatg gcattaaaag gtggcttaac agcatttgat caaatttttg ctttaactgg
    11701 tggtggacca agcgatgcca caacatcttt ggggttactt gtttataatt atgcttttaa
    11761 aaacaatcag tttggttatg cgaatgctat tgctttagtc ttatttatct taattgggat
    11821 tgtttcactg gcacaattaa aattatcgaa aaaatttgaa atctaggagg tttcccaatg
    11881 aaaacatcaa aagaaaaaaa tatttctaaa tacttgcttt tattattagg gggcttcatg
    11941 attttagttc ccttagtttc aacgctattc ggttcattta aaacaacacc cgatattatg
    12001 aataatttct ttcgttttcc taatccagcg actttggaaa atttccgaag attgttagcg
    12061 gatggaattg gtcattactt ttggaattcg gcaatcatca caatcattgc agtagttgct
    12121 gtgacagcaa ttattcctat ggctgcttat tcactagcaa gaaaaatgac tcgtgggaaa
    12181 gcatacggat tgatgtattt atttttaatt ttaggaattt ttgttccttt ccaagtaatt
    12241 atgattccga ttaccgttat gatgagtaaa attggtttag caaatatgtg gggattaatc
    12301 ttactctatc tcacttatgc cgtaccacag acactatttt tatatgttgg ttttattaaa
    12361 ctaaatgtgc ctatcagtct ggatgaggct gctgaaattg atggggctag tcactttaca
    12421 acttatttca aaattgtttt cccaatgtta aaaccaatgc atgcgacgac tattattctt
    12481 aatgctctat ggttttggaa tgatttcatg ctaccattat taattttaaa tcgtgattct
    12541 aatatgtgga cactaccatt gtttcaatat aattatacgg gacaatattt taacgactat
    12601 ggtccaagtt ttgcttccta tacagtagga attgtcacga ttacactagt ttatcttgct
    12661 ttccaaagaa gtattatagc tggaatgagc aatggagcag tgaaataacc aataaattat
    12721 tctcattata aaggagttca taaacttatg attatatttg acaatcacac acaaacattt
    12781 catttacaaa ataaaaaaat tagctatcta ttaggaatcg aagaaaataa ttatttaacg
    12841 catctttatt ttggaaaaaa gattacgcac tattctggtg ggtatcgcta cccccgaaca
    12901 gatcgttcct tctcacccaa caatgcaaat gcgcaagatc gtttattatc tttagacaca
    12961 ttaccattag aatttccagg atatggacat ggtgatttta gagaaccggc gcataatatt
    13021 aaattaggaa acggtagtcg tgtaaatgat tttcgttatg atagctacga aattcaaaaa
    13081 ggaaagccca gcttagcggg attgccagcc ttatatacgg aagatgatga tgaagctgaa
    13141 acgctgatta ttcaattgaa agacgcgatt agtggattga ctttacagtt gttctacaca
    13201 atttatcaaa atcacagtgt aattattcgt tctacgcaat tagtaaataa tagtgatgaa
    13261 acagtggaga ttaaccgctt agctagtcaa tcaattgatt ttccaaatcg tccactcgag
    13321 ttaattcatt taaatggtac ctgggcaaga gaacgtcaaa tgactagaga aaaaattcat
    13381 acagggataa aagtattaga tagtaaacgc ggatcaagca gccatcaaca aaatccgttt
    13441 gttgctttag tagatgccaa aacagatgaa tttcaaggag aggcatatgg atttaattta
    13501 gtttatagcg gtaatcacga aacggtgatt caaaaagatc catttgacca aacacgggtc
    13561 attatgggaa ttaattcatt taattttggt tggcaactaa aaactggcga ctcatttcaa
    13621 acgccagaag tcgtgatggt ttatagtaat gaaggtctga atacgttatc acagacgtat
    13681 catgaattgt ataatcaccg tttaattcgt ggaaactatc gtttaaaaga acgtcccacg
    13741 ttaatcaaca actgggaagc aacgtatttt gattttaacg aagaaaaaat catgaatatt
    13801 gtcgatgatg cgaagaactt gggcattgaa atgtttgttt tagatgatgg ttggtttggc
    13861 ggtcgtgaaa atgacttcac ttcattaggt gattgggttg aaacagaagg caaacttgct
    13921 tgtggtttgg aaaatttagc caagcaagtg catgataacg ggatgaaatt tgggatttgg
    13981 tttgaaccgg aaatgatttc tgaagatagt gaactatttc gagcacatcc agattgggca
    14041 ttgggtattc caggtcgtga ccgctcaatt agtcgaaatc aatatgttct tgatttttca
    14101 agacaagatg ttcgtgataa tatctatcaa caaatgacgg cgatttttga tcgtgtaccg
    14161 attgattatg tgaagtggga tatgaatcga aatatgacag aagtctattc tgtcttgttg
    14221 gactcaacga tgcaaggtga agtggctcat cgttatatgt tagggttata tgaattttta
    14281 gaaaagttaa caacgaatta tccaactatt ttatttgaaa gttgttcggg tggaggcggt
    14341 cgttttgatg caggtttctt gtattacatg ccacaaactt ggacgagcga caataccgat
    14401 gccgtggctc gtctgaaaat tcaatatgga accagcttag tgtatccaat ttcaagtatg
    14461 ggtgcgcatg tttcagcagt cccaaatcat caaacaggaa gagaaacgag tttaactatt
    14521 cgtggcaatg ctgcgatggc tggtgttttt ggttatgagc tagatctaac gcaattaact
    14581 tctgaagaaa aagcagaaat taaagaacaa gttgcctttt ataaaaagca tcgccaactt
    14641 ttgcaatatg gaacttttta tcgcttggaa agtccgtttg aaggcaatga tactgcatgg
    14701 atgtttgtct caaaagacca aacagaagcc atggtctttt atttccgagt attagcagaa
    14761 gctgcgcatc cattagtcac attgaaatta gcaggattag cagaagaagc aacttatcaa
    14821 atggaagatg taacatttgc tggcgatgaa ttgatgaatc tcggttttta tattgactca
    14881 gaactacatg gtgattatgc gacacaacgc ttttatttga aacagatata gtagcttgaa
    14941 tttacaatat aagtgcaaca ccaaaagaga ctcattccaa acgaagaaaa tatgttttat
    15001 ttacacgcat ttactaaaaa gcagcctgat ttgaattggg aaaatgaaga agtacgtgaa
    15061 gaaatctatg caatgattaa ctattggtta gataaaggac taggcggatt tagaatcgat
    15121 gcgatattaa acatcaaaaa aaaactagag tatggcatct ttgaatctga tggagaagat
    15181 gggttagcct tcattgggcc ctggattctt aatcagtcgg gcattgaagt gtggttaaaa
    15241 gaaatgagag agcggacatt taaaccacat aatagtatga cggtagcaga agccgatgtt
    15301 cctaatgaac gtttagatga atatattggt gaagatggct attattcgat ggtttttgat
    15361 tttagttata cggatattga tgtacctgaa actggtgaat ggtttaaaga tagccaatgg
    15421 acatggacgg atatgcgtac caatattttt acgaatcaat tagttacgca agataaaggt
    15481 tggggcgcct tatatttaga aaaccacgat caaccacgtt caattaataa atatattcca
    15541 gaagaatgga tcaatgatta tagtaaaaaa atgttggcga cgctttttat gttactaaga
    15601 ggtacccctt ttatttatca aggacaagaa ctaggaatga ccaacataga aatggcatca
    15661 ttagaagatt ttgatgatgt agctacgcat tctcagtata aacgggcatt agaatatgga
    15721 ttggcaccag agcaagcatt gaaagctgtc aataaacgaa gtcgggataa ttctcgcaca
    15781 cctatgcaat ggaatacgca aaaaaatgcc agattctcaa cttctgataa tgtatggtta
    15841 aaagtaaatc ctaattatcc agaactcaat gcagaagctc agttaaccaa tcaacagtcg
    15901 gtattcaatt attatcatca attaattaaa ttaagaaaaa atagtgtata caaagacgtg
    15961 ctaatctacg gacaatttct accagtgaaa gataaaaatg aacatatttt gctttatgaa
    16021 cgccaattag aagataaaat cgtattagtt atcttaaacg ttacagcaac agaacaagaa
    16081 tattatgtag atgaacagta ccaacaagta ttaatcaata attatgaaga tgttttatta
    16141 gatcaaagtc gaaaattgcg tttacgaccg tttgaaagta ttgtattagc taattatgga
    16201 ggacaattat gaaaattaaa aacgaagcaa tgctcatcac ctatgcagat agtttaggaa
    16261 aaaacatggg cgaattaaaa caagtcttag acaaacattt acaaggtgtt attggtggtg
    16321 tacatctgtt gccttttttt ccatcgacag gtgatcgagg ctttgcgccg agtgattata
    16381 cgacagttga tccagctctt ggaacatggg aagatgtgga agcattaggt gaagattatt
    16441 atttgatgtt tgattttatg attaatcaca tttctcgtga atcaaaattt ttccaagatt
    16501 tcaaaaaaaa ccatgagcag tcaccataca aagaaatgtt tatccgtatt catgaatttt
    16561 tcccagaaaa tcgaccaaca caagaagata ttgatttaat ctacaagcgt aaagacaaag
    16621 cgccgtttca aacggtagaa tttgcggatg gtacgacaga agaggtttgg aatacctttg
    16681 gggaagaaca aattgattta gatgtgacca aagaagttgt gaaagaattt attcgagaaa
    16741 ccatcaaaga tatggcgaac catggctgtt ctttaattcg attagatgcg tttgcttatg
    16801 cgattaaaaa attagatacc aatgatttct tcgtggaacc agatatttgg gaacttttag
    16861 acgaagtgcg tcaagaagca gcgaaatatg acgtcgaatt attaccagaa atccatgagc
    16921 attattccat tcaaatgaaa attgccaatc atgattatta tatctatgac ttcgccttgc
    16981 caatggtcac tctgtattca ctttatagtg gaaaatcaga acgtttagcc aattggttga
    17041 aaatgagtcc gatgaagcag tttacaacat tggatacgca tgatggaatt ggtgttgtgg
    17101 acgctcgtga tttattaaca gatgaagaat tggattacac ctctgaggaa ttgtataaag
    17161 tcggggcgaa tgtgaagaag gtctattcaa gtgccaatta caataattta gatatttatc
    17221 aaattaacag cacctattat agtgcattag gcgataatga ccgtagctat ctgttagcac
    17281 gtgcgattca agtatttgcg ccagggattc cacaaattta ttacgttggg ttattagcag
    17341 gaaaaaatga tattgaactg ttggaagcaa cgaaagaagg gcgtaatatt aatcgtcatt
    17401 actatgattt agccgaaatt gaagaagaag tgcaacggcc agtgattcaa caattatttg
    17461 cacttttaaa atttagaaat gaatctgcgg cgtttgattt ggatggaacg attgaagtag
    17521 caacgccatc agaatcagaa atagtgatta cacgtaaaaa taaaaacggt agtgatagtg
    17581 caacattaac ggctaacttg gatacgaaag aatttatcat tgagtctaaa gatataaaaa
    17641 ttttcactag tgttgcatta aaattttaac ctattccata gaacaaaaaa tcctttatac
    17701 tagcacttgg atgacttatc caagaccaat aaaaaggact catacatgga taagtataaa
    17761 acaaatacag catttaataa atggttttct gccataaaat taggaaaact ttctgtcaac
    17821 tcccaaaaat gtatttttga ttacaattcg tatcgtaaaa aactgtcttt tgaagctgtg
    17881 ctcaagctct ttctctacgc agttaatgaa gaaaaagaaa gtttgcgtga tttgagtaca
    17941 gctcttatcg accgatcgct ccaactagaa accgatgtgt catcgattag tcatacccaa
    18001 ttgtctcgag catttaatgc acttgaacct aaagtgttag aagaaatttt tcaacaactg
    18061 ttagaaaaag ttcaacatca aatcaaacca accaagcgaa atcatctgta tctgattgat
    18121 tccagtacgt tttcgcttag tttaaaacgt cataagtggg cgactttccg tcaaacaaaa
    18181 tcaggtgtca agttacactt aaactattgt tatatggacg atactacgat gtatccaact
    18241 gattttacct taacaaatgc ccatgaacat gatctcaatc aactaccagt attggtgaat
    18301 caaccagagg caacttacgt gtttgatcgt gggtattagg actttgaaaa gatggatcag
    18361 atgcattggg atggctattt cttcgtcact cggattaaaa aaaacaccaa agttcacgtg
    18421 atagatactc tggaaacatc gccagaaaca gaaattctac gagacgagct ggttcgttta
    18481 ggatctaaaa cctatctaac cgccaatttc cgactagtca ctgttcaaga taagaacgga
    18541 cgtgtgtttc aatttatcac caatcgaatg gacgtctctt caaaagaaat ctctgatatg
    18601 tatcacgctc gttggcaaat cgaattgttt ttcaagcaca tcaaacaaca catgaccatt
    18661 aaaaccttct tttctcaaag tgaaaaaggc gtgcagaatc aattgatttt aacgatgatt
    18721 tccgctctcc tcacgtttct catcaaattg gagacgaaaa cggaaaaatc agtgttccaa
    18781 atcaaacgat tctttcgtta tttactgttt cagccctttg aatgttgtct agaaaaatta
    18841 attcccacat gagaaaaaag aaatttggct cgttactttt ggttttcagt tgaataacag
    18901 ataaaaaatt ttctggaaaa agtcatcctc tgcgtcagca cagttttttt tgacttattt
    18961 tcagaggaaa tagttttcag aatattctaa aatgttttat gcaacactag tgatattttt
    19021 tagaattaga tttctaatgt gcattttttg agtattaagg agcaagtata caaagattta
    19081 taaagctgat taaatttgaa taaaaatatg caggaacgct actattttta gtagtgttct
    19141 tttttatatt tgaataaaaa atgtggtggt taaatggtgg ttataacaaa aaatgaatta
    19201 atgaaattag gttatgggaa gtatcagtct gagagaatca tacgacaggc aaaagcaatt
    19261 atggtttcaa aaagctatac ctattacgaa aatagaagat taggactagt acctgttgaa
    19321 tctgtagaag aagttttagg tatgaagata gaggtagagg aggattttca gaatcatggt
    19381 taattttaaa aaagataaaa atgggaaata tcattttact gctagtttgg gattcgacaa
    19441 acgaacaggg aaaagagtac aaaaaaacgt agaggggtta aaacagttaa agaagaaaaa
    19501 aagcgtatac cgaacttgtt tctaattatg gaaaagaagc agaaacttct caaagtacta
    19561 tgttgtttga ggagtttttt tataaattat tcctacctta ttatcaaggg aaagtagaag
    19621 aaagaacata taaaaatcgg ttatctacaa tcaaaaaaac ttttcttatt tctttaaaat
    19681 ggagttaaga gaaatacgtc caattgacat tcaaaagtgg caaaataatt tattgagcaa
    19741 atataaaaat tcttatgtaa gaaacatgta tgggattttt cagatgtcat tagatcgagc
    19801 agttgttttg ggactacttg aaaaaaatcc tgcaaaagtt attggaaatg tgaagaaaag
    19861 gaaaactaca gttgattttt ggacaaaaga agaatttaca aaagttattt caacttttta
    19921 tattgatgat ttgtaccaac attttggttt tatttgtatg tggttacttt ttatgaccgt
    19981 tatgcgtttg ggagaagcca ctgcattaac ttggaaagat attgattttg aaaataaaaa
    20041 cttgcgtgtg aataaaacac tctattataa gagagccaaa acctatgagt ttgtagaacc
    20101 cgaaacggct tctagtattc gtacaatcga actagacgac aatacaatta cctttttaaa
    20161 agcttggaag gagcgtcaag tttcaatagg aggcatgtcg tttgtacttt cttataatgg
    20221 aacacctact cagaaatata caatccgtca tattatgaga agacatgcaa cgatcgttgg
    20281 tgttcatata atccgcatac acggattaag gcatagtcat gcttctttgc ttatatcaca
    20341 ataaaaagat gcacttcgaa ttaaagaaag gttaaaacat gctgatgtac aaacgacttt
    20401 aggaacttat ggtcatttgt atctaaattc attgagagag ataacaaatg atcccacaga
    20461 ttatatccaa actaattttt ctaaaatcaa accgacacct atttacgata ataaaaaata
    20521 aatgataaac aaaagtagga tcacaccaaa agaaacacac tagaaaaact ataatacagg
    20581 cttttctagc gtgttgtttc ttatttccgc ctatttatac attatactca taaagcattt
    20641 aatgcatcga tcgtagccgc attgtctata tgggtatata aagttgttgt attggtactt
    20701 gaatgtccca attgttgggc agttaatacc aatgaatttg tctgttggta gagtctagta
    20761 gctaatgtat gacgcagttt gtgagggctg acttgaattc taaagccttt tgaatatttt
    20821 gctaccatcc gttcgattgc aataccggat atacgcttgg cttctccttg gtaaattgct
    20881 agaaataaag ctttttcgtt ttctgttact ttatatcgtt ctttccgaat agctagataa
    20941 ttagctaaat attccattgc gaatgctgct atattgacag catctctttt atttcctttt
    21001 ctagttacta ccacattatt cttatctaaa gataggtcat ctaaattaat atttataacc
    21061 tcggagagtc tcaatccaga tcctaaaatc agtgcattga tcgccaggtc tctttctaca
    21121 tctcgttgat aatagatcaa agctcgttta ctcaaagtat ttttatattc attggcaata
    21181 taatctagat aattttgtgt atccttatct agaaataatt ttttctcaat ggcattggca
    21241 cgtgcagcta acgtagtttt atctgtgatg gttgctactt gatccatagg attattgtct
    21301 aagtatggtt ttcctagagg attattggaa cttttcgaaa gatatttaaa tagaaccttt
    21361 aatgccgtga tatttctatt gatagtagaa gtagatagac ctaagttatt attatctttg
    21421 agcttagttt ttgttgtctc tttttttaca ttttcttttg tgcgacgttt caaatgagat
    21481 ttgtacaagt ttaggtcata ggcagttaac tgtcttatcg aggtgataga agtatcagga
    21541 acgatagact cactcagaag ccattcataa aagagcttat aattttttag atattctaaa
    21601 gtggttcgag gtgattgatc tttatcctca cgatcttgaa caaaatcttt tatataatcg
    21661 ggcatatgaa gtatgattgt atctattttt tgatatattt ctgtattttt catgtcattc
    21721 atcctttcaa taagtagggg agaagagtat ctctataatt ggactatacc aattatagaa
    21781 tgttttgtca attggtatgc tctaaaattt attaatttgt ttcaaaaaaa gcatataaac
    21841 aggtatacgt ttaataattt aaaataaatg gtatacataa atagggaagg aaatagaagg
    21901 gagcaatcta aattttttga attttcttaa ttaattatat agaaagaaca atctttctat
    21961 agagttagac atgagaggga ctatgcttga tttaaaatca acaagcgtat atactgtata
    22021 tacaaagaaa aaggagggag aagactatgg caattaaaga aagaaaattt agggaagtgg
    22081 ggaactcagt agtattaact atttcaaagg agtttcttaa agaaaatggg ttaaatcccg
    22141 gagatacggt ttttttagat gaagaaaaac ttcgagcggc aattacaaaa aaacaagcct
    22201 tatctgatga aattgattta gcaatatctc aagcattaac tgaatatgat actactttta
    22261 gagagcttgt cgataaatga gtattcatta cctagaagcc aaagatatta taaaaatgaa
    22321 tgtaatccaa ataaaaaaat actctcctaa tgaacccctt ggtgtaaagg atgcgaatgc
    22381 tttagaaatg tgtgtagggc aacttcgagc ctcagctttt ggtgaagagg tttatccttc
    22441 gatttatgaa aaagcagcta tcctacttat acaattgata aagaaacatc cttttcacaa
    22501 tgcaaataaa cgtactgctt tcttagctac ttttgtcttt ttgaaaatca atggacagct
    22561 tttagctatt gatcaaaaag atgctgtaaa tttagcggta tatattgcta cgtatgataa
    22621 ggatttcgat caactaaaat atgaagtgat caacactata aagacacata cgcagcccat
    22681 ttaaggagtt actgtatgtg tctttatagg gttattagct gctatagcga aataattgaa
    22741 gtaaattaca aatagctata attcaaatcc aactatatag aaaacgtaaa ttttctatat
    22801 agttggatta agataacgac tatttatttt atcatatttt tactataccc tatactttta
    22861 cttggtatat ttatgaataa tcgcaaattt acccctatac
//
LOCUS       DARBEC010000044        22187 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.044, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000044 DARBEC010000000
VERSION     DARBEC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22187)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22187)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22187
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.044"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            55..1953
                     /locus_tag="SCL80_001551"
     CDS             55..1953
                     /locus_tag="SCL80_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEG1107421.1"
                     /translation="MDRYLDLLSEKFPTTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETEISQFTIFTAYPEYVLTTDWYKKQDKS
                     QLIHRLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFTP
                     KNRKDLSEREQEELEKIHQALSILQFKSEHQLLNRRPEFKMDDRKLLEKINYEESTIV
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTANEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDAKEEGSAIINGHTPVKVKKGESPIKANGKAFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRNTTKGMELQQQITQLQR
                     LLDYRKDD"
     gene            complement(2252..2971)
                     /locus_tag="SCL80_001552"
     CDS             complement(2252..2971)
                     /locus_tag="SCL80_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEG1107422.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIEKIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFATHEVKIVSD
                     SLLSDLLPDSYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDNMHKILGVQWHP
                     ELSHRVDSSEQRLFDFFVNEF"
     gene            3308..3640
                     /locus_tag="SCL80_001553"
     CDS             3308..3640
                     /locus_tag="SCL80_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107423.1"
                     /translation="MKQVLEALEQIRKAEEENEKRARTLRQQLKTYAEEKEQELEIIQ
                     TNSRQQLKQLVEEKEQFEKQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            3627..5630
                     /locus_tag="SCL80_001554"
     CDS             3627..5630
                     /locus_tag="SCL80_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEG1107424.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFMNKLEEVLDLKEKWEQLTEKRDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAAIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKVKERFIHATYLIVLQGWVDAEEKQNLMHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLIEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLFVLPRGMKRFVKFFEILSVPSIIWGLIYSSFFGQALPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALIGVMLIKNTAFVYLGAGLSIIAALCILIVPIIQSPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            5649..6119
                     /locus_tag="SCL80_001555"
     CDS             5649..6119
                     /locus_tag="SCL80_001555"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEG1107425.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            6140..6724
                     /locus_tag="SCL80_001556"
     CDS             6140..6724
                     /locus_tag="SCL80_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEG1107426.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQIETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAIAEMENWDESEQ
                     IQFIKNSLYSLPLTGKVTFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            6739..7725
                     /locus_tag="SCL80_001557"
     CDS             6739..7725
                     /locus_tag="SCL80_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEG1107427.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNLDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFEPETIRKRVRQVYDA"
     gene            7715..8026
                     /locus_tag="SCL80_001558"
     CDS             7715..8026
                     /locus_tag="SCL80_001558"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEG1107428.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            8048..9829
                     /locus_tag="SCL80_001559"
     CDS             8048..9829
                     /locus_tag="SCL80_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEG1107429.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            9822..11198
                     /locus_tag="SCL80_001560"
     CDS             9822..11198
                     /locus_tag="SCL80_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEG1107430.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            11205..11840
                     /locus_tag="SCL80_001561"
     CDS             11205..11840
                     /locus_tag="SCL80_001561"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEG1107431.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            11870..13225
                     /locus_tag="SCL80_001562"
     CDS             11870..13225
                     /locus_tag="SCL80_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEG1107432.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(13417..14811)
                     /locus_tag="SCL80_001563"
     CDS             complement(13417..14811)
                     /locus_tag="SCL80_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEG1107433.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRAQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            14903..15874
                     /locus_tag="SCL80_001564"
     CDS             14903..15874
                     /locus_tag="SCL80_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEG1107434.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            15871..16653
                     /locus_tag="SCL80_001565"
     CDS             15871..16653
                     /locus_tag="SCL80_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEG1107435.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(16822..17193)
                     /locus_tag="SCL80_001566"
     CDS             complement(16822..17193)
                     /locus_tag="SCL80_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1107436.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            17327..17770
                     /locus_tag="SCL80_001567"
     CDS             17327..17770
                     /locus_tag="SCL80_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEG1107437.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="SCL80_001568"
     CDS             complement(17813..18622)
                     /gene="yidA"
                     /locus_tag="SCL80_001568"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEG1107438.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(18641..20008)
                     /locus_tag="SCL80_001569"
     CDS             complement(18641..20008)
                     /locus_tag="SCL80_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEG1107439.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(19992..20822)
                     /locus_tag="SCL80_001570"
     CDS             complement(19992..20822)
                     /locus_tag="SCL80_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEG1107440.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            20985..21422
                     /locus_tag="SCL80_001571"
     CDS             20985..21422
                     /locus_tag="SCL80_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEG1107441.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTGGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            21498..22118
                     /locus_tag="SCL80_001572"
     CDS             21498..22118
                     /locus_tag="SCL80_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEG1107442.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
BASE COUNT         7118 a         3850 c         4782 g         6437 t
ORIGIN      
        1 atagagcata acaactttat aatgttgagt ataccaattt ttggaggcgt tttaatggac
       61 cgctatttag atttactttc agagaagttc cctacaacgg aagatgtcat cacggaaatc
      121 atcaatctcg aagcaatttt gcagcttcca aaaggaacag agttattttt gagcgatata
      181 catggcgaat ttcctgcttt tgatcatatt ctgcgtatcg gttcaggaaa tcttaaagaa
      241 aagatcaaag accttttctc tgaacaaatg acggaaacgg agatcagcca attcactatt
      301 tttacggctt atccagaata tgtgttgacg actgattggt acaaaaaaca agataaatcc
      361 cagctgatcc atcgtttgat cgatctttta cggtttacga cagtcaaata cactcgatca
      421 aaagttcgaa aaggattgcc gaaagaatat agctatatca ttgaggaact cttgtatatc
      481 gatgatcgta tcaacgggaa aaaagactat gtaaaaaaaa tcattgaaca acttgtcttg
      541 atgaaggaag aagaaaaatt tttaacaaaa cttgcgcaga cgatccaaaa atctgtcatc
      601 gatcatttgc atatcgtggg tgatattttt gatcgaggaa ctcaagcagc caaagtaatg
      661 gatcgaatca tggatttttc ctctgtagat atccagtggg ggaatcatga tattttgtgg
      721 attggtgcgt attgcggctc tgaggcatgt ttattgacgc ttttacggat tgccgcacgc
      781 tacaactatt tatttgaatt agagaaagaa tatggattaa atttacgtcc attattttct
      841 tttgcccatc aaacctatca gaagaatcca gtatttaccc ctaaaaacag gaaagatcta
      901 tctgaaagag agcaagaaga attagaaaag atccatcaag cactttctat cctccaattc
      961 aaatcagaac atcagttgtt gaatcgacga cctgaattta aaatggacga tcgtaaattg
     1021 ttggaaaaaa tcaactatga ggaatcaacg atcgttttag aaggtcagct ctatgagttg
     1081 aaagggactt gttttcaaac gatccagcca gatgatccta aaaaattctt ggaagaggaa
     1141 caaaaagtga tcgatagttt gatgtattca ttccagcact cgttaagaat gcaaaaacac
     1201 atgacgtttt tgatcgaaaa agggggaatg tatctcacga acaatcagca tgtactgttc
     1261 catggctgta tacctgtaga tgaaaagggg cagctcttag catttgaatt agaccaatct
     1321 tatcgcggaa aagaactgct gggatttttt gaaaaacaga taactgaaag cgcaaaagat
     1381 ttgactgcaa atgaagattt ggcgaccgat ttgatttggt atgcgtggag cgggccattt
     1441 tcgcctttat tcggcaagag taaaatggca acttttgagc gctattttct cacagagcct
     1501 catacgcacg tcgaaaaaag caatccctat tatcacttgc gagatgaaga gtggttcagt
     1561 gaaaaaattt tggcagaatt cgatgcaaaa gaagaaggtt cagcaatcat caatggacac
     1621 acgccagtaa aagtaaaaaa aggagagtca ccgatcaaag caaacggaaa agcatttgtt
     1681 attgacggag gtctatccaa agcttatcaa aaaacaaccg ggattgctgg ctactcatta
     1741 ctatgcaatt cttacggttt ccaaatcgtg acacattatc cgttcttgcc tattgaacaa
     1801 caatttgcaa aaaaatctga tcaaacgaat gtaaaaaaag tgatcgagca gcagctccca
     1861 cgcaaactta atcggaatac gacaaaagga atggaattac agcaacagat tactcaactc
     1921 caaaggctgt tggattatcg aaaggacgat tgagaaaaat gctagccaac attgctcaat
     1981 agactaaaaa gcgactgcaa caaaaggcgt gtcgcagtct ccttcggaat tagctcaaaa
     2041 aacggtaaaa tattagaagc tatttttctg tttatttctc taatactcag gtcagggcgc
     2101 tgatttcacg acctcttcct taaacggtgt tctcctgcca actcctcggt cacaagtcac
     2161 aatatccaac aaacatgaga agctgttttt tgaaattgct ccttgtgact cagagttgaa
     2221 cggcaggctc acgacctaat cagaaaaacg attaaaattc attcacaaag aaatcgaata
     2281 aacgctgttc agatgaatct acacgatgag aaagttccgg atgccattgg acaccaagga
     2341 ttttgtgcat attatcttta ctttggactg cttcaatgag tccatcgttg gaaaaggcga
     2401 tggcttttaa caaaggagaa agatctttga ctgcctgatg atgataggaa ttcacaaagt
     2461 atgaatcagg gagaaggtca cttaataaac tgtccgaaac gattttgact tcgtgagttg
     2521 caaactgcgg ggcagtgggc tgttgttcgt gttttacgga ccattttgga tataatgaaa
     2581 gatcttgata aagtgttccc tccaacacga cattcaatag ttgaagtcct cggcagactg
     2641 caaaaatcgg ttttctttgt ttcaatgctt ccaagatcaa tgcctgttcg aattggtctc
     2701 gttgaatatt tgtttccgtc agttttggat gagggtcttc catgtaaagt tgcggactaa
     2761 catcctgacc gcctgcaaga atgagtttgt cgattttctc aattagttga ggcgctgaat
     2821 ctggagacat gactggcaga ataagaggta atccttgagc atctaaaact gcatccacaa
     2881 atccctgcgg tgtatagctt acttgatttc cttggaaagt atctgtcgct cgaatcagtt
     2941 gattcccggc aatgccgatg attggtttca tttttattcc ccctaaagta gactaattgt
     3001 aacaaggatt taaaaaaatt taaactatgt aagacttgcc cctatactta tctgttcttg
     3061 ctgtgttact atctgtaaag agaattttcg tgtgaaattc atgacgatag ttgaaaatat
     3121 tgcttaattt gttataataa aaagtaagca gaaataaaaa ggttgggcgt aagcttcaag
     3181 gatctagttc ttcaaggggt gagaaggaca ttttattgaa ggggcctgcc tatactaagt
     3241 tatctttaga ggtacatact ttcaatgaaa aaagaaagca tgtacctctt ttttaaatcg
     3301 aaaggaagtg aagcaagtgt tagaagcttt ggaacaaatc cgaaaagcag aagaagaaaa
     3361 tgagaagcgt gctcgaaccc tacggcagca gttgaagacc tatgcagaag aaaaagagca
     3421 agaattggag ataatccaaa cgaacagtcg gcaacagctg aaacagttgg tagaagaaaa
     3481 agaacaattc gaaaagcaag aattagaaaa agaaaaaatg aaattgaaag cagaagcaga
     3541 caaggtgcaa gcaactttga aagaccaata tcaaaagcat cagagtcaag caatcggtgc
     3601 aatcattgaa agggtgaaag aaacttatgg ccgtcactaa gatggaaaaa gtgaccctca
     3661 tttcagataa aagaaatcag gagaggattt tacaagcagt acaaggaatc caaaatgttg
     3721 aaatccggga tctgtatcaa aatagaacaa acaaccagtg ggtagaaaaa tattttccag
     3781 ataccacgat tattgataaa gaagcaaaat taagcgcttt aggcaatcgg ctgaaagaca
     3841 tccaagaagc tatccaattc atacaacacc acggcgatag ccgtcaaaag aaactccatt
     3901 tgaaacgtca agaactttcc ttacaagact tggaagcaaa ttattccgaa gaaaatttca
     3961 tgaataaatt agaagaagtc cttgatttaa aagaaaagtg ggaacagcta actgaaaaac
     4021 gtgatcgttt agaagaagag gaagaatggc ttcttcattg gcaacagctg gatattgctc
     4081 caagtatgta tgcaagtaag acgaccattt ttgagatggg gactgtgagt gcagcaaatt
     4141 ttgaaagttt caaggaagaa ctaagtaagc ttgatgacgt ttatatggaa gaaatagatt
     4201 atacgccaaa acaagtttat ctttcgtatg ttgctttaaa aaaatcagtt gctgctatag
     4261 aagaaattgc tgggaaatac gggttcaata aagaaacgta tccttatgaa caaaatccta
     4321 aagaaaaatt gcaagaaacc aaacagcaat tatccattgt tcacgagcag caaaaaaaga
     4381 taacgacagc gttaggtcag tgcagcggct atatccaaga atttgaatgg gtagaagaag
     4441 tcacacttgc cgttgcagaa cgggaaaaag taaaagaacg gtttatccat gccacttatc
     4501 tgatcgtttt gcaaggttgg gtagatgcag aagaaaaaca gaatttgatg catgtgttaa
     4561 acgaaactgt atcagaagaa gatatatacg ttagtttcga aacaccaaac tcagaagaga
     4621 ttcagcagga agtccctact aaattgaaaa accatccgct tatcgaacct tttgaattgc
     4681 tgactgaaat gtacagtctg ccaaaatatg aagaagtaga tccaacgcct tggatgacac
     4741 cattttatct tgtcttcttc gggatgatgg tcgcagatgt tggttacgga ttgctgatgc
     4801 tcattgggac aatactagct caaaaactat ttgttttacc ccgcgggatg aaacgtttcg
     4861 taaaattctt tgagattctc tcggtaccat cgatcatctg gggattgatc tacagttcat
     4921 ttttcggaca ggcattacca aaagaactat ttggtatccg cttgcctttc ccaatcctat
     4981 ctacgacgga tgacgtcaat acgattttga ttttatccgt gattttcggt ctgatacaga
     5041 tcttggttgg attattcgtg gcagcaaagg aaaatatcag gaggaaagat tacttgagtg
     5101 ctgtcagtga tggttttgcc tggcaaggga tcctgattgg gatcgttatt gcattgattg
     5161 gtgtcatgtt gataaaaaat acagcatttg tttatcttgg cgcggggcta tcgatcattg
     5221 ctgctttatg tatcttaatc gtgccgatta tccaatcgcc gtctaaagtc aagggtgctg
     5281 ccaaaggagc gtataatctt tacggattga ctggttatat cggtgattta gtcagttata
     5341 cacgtttgat ggcactaggt atttcaggcg gaagtattgg cgcggcattc aatatgctag
     5401 ttgctttcat gccgccagcc gctcgattca gtgtagggat ccttttgatc atcgctttac
     5461 acgcactgaa tatgtttttg acactgttaa gtgcctatgt ccatggtgca cgattgcagt
     5521 atgtcgaatt ttttggaaaa ttttatacag gaggcggacg tgcctttgac ccgctgaaaa
     5581 cggcggaaaa atacgtcaac atcaatcata agaaaaaaaa caataaataa ttggaggatt
     5641 tttatacaat gatggattac ttaattagtc aaaatggtgg aatggtattt gcagtattag
     5701 cgatggcaac agcgacgatt ttttcaggga ttggttcagc taaaggtgtt ggaatgacag
     5761 gtgaggcagc agcagcgtta acaacaagtc aacccgaaaa attcggacag gctttgattc
     5821 tgcaattgct tccaggtacg caagggttgt atggattcgt tatcgccttt ttgattttta
     5881 tcaacttagg aaatgatatg tcagttgtac aaggattgaa ctttctaggc gcttctctgc
     5941 cgattgcttt tacaggtttg ttttcaggta tcgcccaagg gaaagttgca gcagcaggta
     6001 tccaaatttt agcgaaaaaa cctgaacatg ctacaaaagg aatcattttt gctgcgatgg
     6061 ttgaaacata tgcgatttta ggtttcgtta tttccttcct attagtattg aatgcctaag
     6121 cataaaggag gaaaagcaga tgaacgccat tgaaaaaatc atctctcaga tgaatgaagc
     6181 agcagagcaa gaaagagcgg cgttagagca agaagagcga atgaagatcg atcaaaactt
     6241 tgaacaaaaa cgtacgcaaa tcgaaacaga acatcaaaaa caaaaagaaa aacaaatcga
     6301 attacttgaa aaaaaatacc gtcagcttag gaatcgccaa caagtagaag tacgacaaga
     6361 aaatttgaat gcgaagcagg aatttttacg acgattgttt gcagatgcaa tagctgaaat
     6421 ggagaattgg gatgaatctg aacaaatcca gtttatcaaa aattcattgt attcattacc
     6481 tttgacaggc aaagtaacat tcattgctgg agaaaaatca gcagcttatc tttctcagac
     6541 attgctggat gaatggaaca atgaacttcc ctttatgatg gttttaagtg atgaaactgt
     6601 tgctgatcaa gcgggttttc tgataaacga tcaaggagtt cagtataatt ttcttttcag
     6661 cagccttgta caagatatcc aaggaacgat gagttttgag attgccaatc aattattcga
     6721 ataaggaggg atcagtgtat ggaataccac gaactaaatc ccttgattcg cggaagagag
     6781 cttgaacttt tgtccaatga gacatttgaa cggatgattc aaacaaattc tgtcgaagca
     6841 cttggtgaaa tattgaaatc cacgatttac agtccatata tttatgacgg atatgaatat
     6901 gacttcgaac agaatctttt aaaggagcag agccgtctat ttcgctggtt aaaggaatca
     6961 gcaccggagc ctgaaatcgt ttggatctat acgatgcgct atactttcca taatctgaag
     7021 gttttgacaa aagccgagat gacaaatcaa aatctggacc atttgtatat cgacgatgga
     7081 ttttattcat tagaaacctt gaaggatgcc attcatacgc aagtttcaaa tgaactccct
     7141 gaaagcatta tggacagtat acgagaagta cgtgattatt ttgaagagtc taccattctt
     7201 cagggaattg acgtgattta tgatcgacag tttttgacag agcagcgacg gcttgccgat
     7261 aagctaggat atcaagaatt gctgacagaa gtcattgctt ttattgactt gaccaatatc
     7321 acaacgatgg ctcgaggaat attgcagaaa agaacatatg ggttcatgac gacggtactt
     7381 tccagctcgg gaagtatcga aaaagaagta tttttgaact ttgtagataa agatatggaa
     7441 gattacacga attttctatt agagacagat tatgctgatt ttctgcgccc tgtcatccgt
     7501 gagggaaatc ttgatctcgt aaagcttgaa cagttgaaag atgattatct aagttcattg
     7561 tatcaagaag cacaaaccca ggctttcgga ccactgccac tactagcctt tttgaacgca
     7621 aaagaggtag aaagcaaaaa ccttcgttta ctggttatcg gcaaaagaag ccatttcgaa
     7681 ccagaaacaa tcagaaagcg ggtgagacaa gtctatgacg cataagatcg gtgtagttgg
     7741 cgacaaagat tctgttttgc cattcaaatt gtttggcttt gatgttcgtt atggtacaac
     7801 caagcaaacc gttcgtcagg caattgaaga aatggcaaag aatgagtatg gcgtgattta
     7861 tgtcacagag caatgtgcag cattgagccc agaaacgatc gaacgttaca aggaacaaat
     7921 gatacctgct atcgtattga ttcccaatca tcaaggatca ttagggatag ggctggaaga
     7981 aattcaaaaa aacgtagaaa aagcagtagg tcaaaatatc ttatagtatg aaaaggagat
     8041 agaaattttg caaattggaa aaatcataaa agtctccgga cctcttgtaa tggctgagaa
     8101 catgtctgat gccagtatcc aagacatgtg tctagtggga gatctaggag ttatcggaga
     8161 aattatcgag atgcgtggcg atgtggcgtc tattcaagta tatgaagaga cttctggaat
     8221 tggtccaggc gaaccggttc gctctactgg tgaagcttta tctgttgaat taggaccagg
     8281 gatcatttca caaatgtttg acggaatcca acggccatta gataccttta tggaaatcac
     8341 acaaagtaat ttcttagggc gcggagttca attgcccgca ttggaccatg agaaaaaatg
     8401 gtggttcgaa ccaacagtag aagccggaga aacggtgagt gctggagatg ttatcgggat
     8461 cgtagaagaa acaaaagtta taaaacataa aatcatggtg ccaaatggga taaaaggaac
     8521 gatcaaaaca atcgaaaaag gttcattcac tattgatgaa acagtatgtg tcattgaaac
     8581 cgaaaatggt gacaaaaatt tgacgatgct tcaaaaatgg ccagtaagac gtagtcggcc
     8641 aatcaaagaa aaattaaacc cggatgcacc catgatcact ggacagcgag tgattgatac
     8701 attcttccca gtgacaaaag gaggagcagc agctgtacct ggtccttttg gtgcagggaa
     8761 aacagtcgtt cagcatcaaa ttgcaaaatg ggccgatgtg gacatggttg tctatgtggg
     8821 atgtggcgag cgtggaaatg agatgacgga tgttttaaat gaattccctg aattgattga
     8881 ccctaataca ggagaatcgt tgatggaacg tacggtattg attgctaata catcaaacat
     8941 gccagtagca gctcgagaag cttctatcta tacgggaatc acaattgcgg aatatttccg
     9001 tgacatgggt tatgacgttg ctatcatggc agactccact tcacgatggg cagaagcact
     9061 tcgtgagatg agcggacgtt tggaagaaat gcctggtgat gaaggttatc cagcttatct
     9121 tggttcacgg cttgcggaat actatgaacg atctggaaga gtcattgcat taggatcaga
     9181 aggtcgcgaa ggaagtataa ccgcaatcag tgctgtatcc ccgtctggag gggatatttc
     9241 ggaaccagtc acacaaaata cattgcgggt cgtaaaagta ttctggggtc ttgattccag
     9301 tttagcccaa aaaagacatt ttccttcgat cgattggata caaagctatt ctctttattc
     9361 aacagaagta ggaaagtata tggatcatat ccttcaagct gactgggcat ccatggtcac
     9421 cgagggcatg cgtattttgc aggaagaaca gcagctgaat gaaatcgtgc gtttggtagg
     9481 gattgattcc ctttcagata atgaccgtct gacattagaa gtggcaaaat ccattcgtga
     9541 agattatttg cagcaaaatg cttttgatga tgtcgataca ttcacttcaa gagagaaaca
     9601 attcaaaatg ttgaagatga tcttgacctt tggtgaagaa ggacgccgtg cgttgtcgtt
     9661 aggtgcttat ttaagtgaga tcatggacgg taccgtatcg attcgtgaaa ggatcggacg
     9721 aagcaaatat atcccggaaa cagaattaga aaaactagat caaattattg gtgaaatcaa
     9781 gcaaacgatg caaacgatcg tttcagaagg aggcatgagc aatgattaaa gaatatcgta
     9841 cgatcggtga agtcgtcggt ccattgatgg cggttgaaaa agttgctggt gtcaaatatg
     9901 aagaattaat cgaagtccgt atgcaaaatg gcgagattcg tcgtggacaa gtattggaag
     9961 tccaagaaga taaagcaatg gtccaaatat tcgaaggaac aagtggtatc aatttacgtg
    10021 actcttctgt acgattttta ggccatcctt tagaactagg tgtatcggaa gacatgatcg
    10081 gtcgagtatt cgatggtttg ggacgaccga aagataacgg accagagatc ctgcctgaaa
    10141 agcatttgga tattaacgga gaagtaatca atccagtagc tcgggattat ccagatgagt
    10201 ttatccaaac aggtatctct gcaatcgatc atttgaatac attagttcgt gggcaaaaat
    10261 taccagtatt ttctggctct ggtcttcctc ataaagaatt agctgctcag atcgcgcgac
    10321 aagcaacggt actcgattca tcagatgatt tcgccgtagt ctttgctgct attggaatca
    10381 cttttgaaga agctgaattt ttcatggaag attttcgtca aacgggcgca atcgaccgtt
    10441 ctgtcatgtt tatgaattta gcaaacgacc ctgcaatcga acgtatcgct actcctcgta
    10501 tggctttgac tgctgcagaa tatcttgctt atgaaaaggg aatgcatgtt ttggttatca
    10561 tgacagacat gaccaactat gcagaggcat tgcgagagat ttctgcagca cggcgagaag
    10621 ttccaggaag aagaggatat cctgggtatc tttacacgaa cttagctact ttattcgaac
    10681 gagcaggaag aatccgcgga ctaaaaggat cggttactca aattccgatt ctaacaatgc
    10741 cagaagacga taaaactcat ccaatccctg acttgactgg ttatattaca gaaggacaga
    10801 tcatcttgac tagagagctt tacaagagtg ggattcagcc accaattgat gtactcccgt
    10861 ctctttcgcg gttaaaagat aagggaactg gacccggaaa gacaagagaa gaccatgcag
    10921 ctacgatgaa tcagcttttt gcagcctatg cacaaggaaa acaggcaaaa gaattagccg
    10981 tagttttagg tgaatctgct ttatctgatg tagacaagat ctatgcgaag tttgctgagc
    11041 ggtttgaaaa agaatatgtc aatcaaggat tctacacgaa ccgttcgatt actgaaacac
    11101 ttgatctagg gtgggaactg ctatcgatgt tgccaagaac cgaattaaaa cgaatcaaag
    11161 acgacatgct ggacaaatat ctgccagaag ggaagtgagt tctgatggca cgattgaatg
    11221 tgaatccgac ccgcatggaa ttgactcgat tgaaaaaaca gttgacaact gccacaagag
    11281 ggcataaatt attaaaagat aagcaagatg aactgatgcg tcaattcatc ctgttgattc
    11341 gaaagaacaa ccagctgcgt caagaaatgg aagagtctat gacagaagca atgtctgatt
    11401 ttgtattggc aaatgcttcg atcacggaac ctttcatcga agaattattt gttttgccgg
    11461 cggaaaacgt tgatttgtct atcgtagaca aaaatatcat gagtgtaaaa gtacctgtga
    11521 tgaattttga atatgatgaa cagctgacag acacaccttt ggaatacggg tatttgaatt
    11581 ccagtgccga actggatcgg tcaatcgatc ggtttactac cctgcttcca aaacttttgg
    11641 aactgacaga aatcgaaaaa acttgtcaac tgatggcgag tgaaatcgaa aagacacgtc
    11701 ggcgagtcaa tgcattagaa tatatgacga tccctcaatt agaagaaacg atctattata
    11761 ttcgtatgaa gctggaagaa aacgagcgcg cagaagtaac aaggttgatc aaagtaaaaa
    11821 atatgggtac cactgactaa gtggtatctt tttttaaaag aggaaaacga tgattaaaca
    11881 aagcaaacaa ataaagaaac gtttgccggc tgttcagctt ctcgcattag gattttttct
    11941 cctgatttta gttggcggcg gcctacttac cctaccattt tttagtaata gtgggcatgg
    12001 gacaaatttt atcgatgcgc ttttcactgc aacttctgct gtctgtgtaa cgggattgac
    12061 aacattgaat acagcagagc attggaacag tgctggtcaa tttttgatca tgcttttgat
    12121 tgagatcggc ggtcttggtt ttatgatggt tccgattcta tgctttgcat tgataaagaa
    12181 gaaagtcagt ctcagcacac gtattatatt aaaagaagcg ctgaatctag aagaagtatc
    12241 aggtgtgatg aagctcatgc tttacatcct aaaattggcc tttgttatcc aattgattgg
    12301 tacggttgct ttatcttttg tatttgtgcc tgaatttggc tggttgaaag gtatctggta
    12361 cagtttgttc catgcaatct ctagtttttg taatgcaggg tttgatttgt taggagacag
    12421 tttagccaat caccagcaaa atgtttattt gatcatggtg gtatctgctt tgatcatttc
    12481 tggtgggtta ggcttcatcg tatggcgtga tttactggaa tatcatcaga aaaagaagat
    12541 gacgattcat tcaaaaatcg cattaaccgt tacaggtacc cttttattag gcggattcgt
    12601 tgtgttcttt ttcacagagc gcaatggggc aacgctagtg gaagggaata tgtttcaacg
    12661 attggcaaac acctttttta tgagtgtgac acctagaacg gctggatatt attcagttga
    12721 ttacctccat atgagccatg caggtctgat tttaacgatg tttttgatgt acatcggcgg
    12781 aacatctggg tcgacggccg gcggattgaa aacaactacg ttaggtgtgt tgctgatcca
    12841 gatgcgttcg atgttgaaag gacgtacgag agcggaagca tttggccgta ccatccgaca
    12901 aggcgcagtg ttgcgggcac ttactttatt ttttgttacg ttgacgttgt gtgtagtcgc
    12961 catcatgatt ttatcggtga ctgaaacgat acctaaaact tctggcatcg aatatattgc
    13021 ttttgaggtc ttctctgcgt ttggtacagt aggtcttacg atgggattga cgccagactt
    13081 gactgcaata gggaaattga tcattatagc gcttatgtac attggacgag ttggtgtatt
    13141 gactgtggtc ttctctctga tgacaaaagc acaaagaaca caatcaaaat acaaatatcc
    13201 agaagaaagt gtcatgattg gataatttca tcttctgaca gaaacctagt gatttagtaa
    13261 aataagaaaa taaaataatt tttcttgctt cttggatact ttaagtgcaa aatgttcgtt
    13321 tattttttga ggcactaccc gttgttgaac ctcgggtagt gcctcttttt tattgaaagg
    13381 aatcaataag atttgattcc tttatttatc aataggttat aattcatatt tttttatttt
    13441 ttcttttacg atacttagtc cagaagctaa aatttcaagg ttattagagg atagggaaat
    13501 tcgaacgaat gcatctggtt gcgcacgtgt agtaaagcgt ctggaatgat aaagtcggat
    13561 accgttagca agaaagtcat tctcaattat cttagggtca atctcaggat caactggaag
    13621 aattcgatag taagggacac gattcgttgg tttttctact ttgaaaagag aatcaaaaag
    13681 ctgattggct ctttttgtaa gacttaattt agtttctaag atcctatttg cttcatttga
    13741 atggaggatt tgagtgatga cttccacatc taatccagaa gttttcacat ttatattaaa
    13801 aattgcttgt tctatcttgc tttggacatg tactggaact acaagataag caactcgcaa
    13861 tccagaacaa ataaattttg tcattccagc taagtagatt gtttggttag gtaataaatt
    13921 ttggaaagga ggaatgatcg tatgttgata gtaagttgtt aaaaatgaat ggatatcatc
    13981 ttcaataacc catagacctt ctttttcaat cacttcggct aattgctctc gtcttttgtg
    14041 agagatttga aaccctatag gattattaca agaaggcata agaaagacac catgaatctt
    14101 cttttttcta cattcctgaa tcaatatatc aggtcgcatt ccttgctcgt caaaatcaat
    14161 tggaacgatc tctaaatgat ataactgagc caattcaatg aaattagcgt atgtatatcg
    14221 gtctgttgct attctatcac ctggagagaa aagtgcagaa agtgtgatag ccaatccatt
    14281 ttgaacaccg gaaacaatag atagagattc taaggacgga atgactcctt gagtttttaa
    14341 ccaatttgct gcagtagtca attgaaaatt tgtgcctttg ggatcttggt aattaagcaa
    14401 accaacaaga tttgggttat tactagttga ttgaataaac gggacaacga atttattaca
    14461 ttcttcaaaa gaactaacca gaccaaaatc aatgatatgt tcggttactt cattggtaga
    14521 gatggtgatg gactcaaaga cattgggaga aataaatgtg ccacttccaa tattcgtata
    14581 cagcaaacct ttctcaattc catatttata agccttgcca acggtagtaa aatttaaatc
    14641 taaataatca gctagctctc tttgggaggg taactgagtg tttttctgta atttattctt
    14701 taaaatatct tgttctaatt gttgaatcaa cgatcgataa taaggacgct tcaatttttg
    14761 tttgtctggt cgccaagtta acgggtaatt gtcataagaa ttaataggca ttttctccac
    14821 tccttaaaat tgtataccat acaattttac cattgattgt attgatttta gaagttaatc
    14881 ttttgtgagg agggaattgt ctatgaaaat tattaatttt gaggaagtag tagcttcttt
    14941 tagttttgaa gaagcgattc aagtaatgaa aaaatgtttt tttgattatg aaagtggtaa
    15001 aatcagccaa aatcctcgat tggtggaaat tttacctgac ggaaaaggca aaaatatgtt
    15061 tgcgatgatg cctgcttatt tgggggagaa cagatatttt ggcacaaaag tcattaccgc
    15121 ttttccggat aatcatcagt atcatttacc ttctcattta ggagagattt tcttgtttga
    15181 tgctcaaaac ggactaccca aagcattgat aaacgcaaat gcagtcacct ggattcgaac
    15241 agctgctgta tctgcgctgg caacagatta cttagcaaat ccgcatgcag aaacacttgc
    15301 tttaattgga gctggtcaac aagcattgtc gcatttgact gcgatttcta ccattcgtcc
    15361 catcactaca gtgtttgttt acgatatttt agaagaaaga cgaaatactt ttatcgctaa
    15421 agctaaaaaa cagtatccta aaataacgtt tataaatgca atatcagtag aagatgcagt
    15481 acgtccagcg gaaatcattt gtacgttgac ctctagtaaa gaggcatttc ttcaaaaaga
    15541 gtgggtaagg gaagacgcac atatcaatgc tgtgggcaca tttactcctg atacaagaga
    15601 gataagtaca tcgttgatgg aaacttctca aatctatgta gatgattact cagctgcctt
    15661 aacagaaagc ggggacctgc ttatagccat ggcagaagga gatttagagg aatcttgtat
    15721 tgttggtaca ttaggcgaat tagtttctga gaaaaaaaca ttttataaag gagaatcgag
    15781 gccgacgatt ttcgacgcag ttggtctagc tattgaagat ctttgttgtg cagaatatat
    15841 ttataacaaa ttagagaaag gggctttaaa atgaacttag attattacgt agttgatgca
    15901 tttgcagatg aggtgttcaa aggaaatcca gcagcggttt atgtgctaga agaatggctg
    15961 ccagaaggta cgatgcaaaa aattgccatt gaaaacaatt tatctgaaac agcatttaca
    16021 gtgaagaaaa accaagaatt tgagcttaga tggtttacgc cagatcgaga aatcgatctt
    16081 tgtggtcacg cgacattagc aacagcattt gttttattta actattacaa aatacctgat
    16141 gaaacgatta aattttctac tcaaagcggt aatctatttg tgacaagaat taaagattat
    16201 tattacatgg attttcctag tattatgccg aaaaaagtgc cgatattgtc tgaatatgaa
    16261 gaagcgattg gggctaagat taaagaagcc tacttagcac gtgatttatt tttcgtatta
    16321 gaagatgaaa aaacggtggc taaattgcag ccagatttta cagctatcaa aaattttgaa
    16381 ttaggaatag gagtcatcgt gacagcagaa agtgtgcaaa aagactttgt ttctagaacc
    16441 ttctttccaa aattaacaat caatgaagat ccagtatgtg gatcagcaca ttctaatctt
    16501 attccttatt gggcagaaag aatgaataaa acaaaattgt cagcttatca aatgtcatca
    16561 aggggcgggg atctagaatg tgaattaaaa gaagatcgtg tgataatagg aggaaaagca
    16621 gttttgtttt ctaaaggtac ctcgtttatt tgatttcttt caaatgaatt aaaaataaat
    16681 tggtggagag tggcaacctt atagctacaa tggttgttaa aaaaagacgc aaactttttc
    16741 aatttaatga aaagtttgcg tctcatttta attattggtg tttgtttttt attcaatcaa
    16801 gagtaaaatg ccccatacta actatcctcg gatgacttcg attttataac catcagggtc
    16861 aatgacaaag tagtaagaag gcgcagtgcc aggtaatcct tttaagtcag taatctcaag
    16921 accggcagct ttatgttgct cgtgtaattt ttctagatca tcagctgcta tagcgatatg
    16981 accatagccg tctcctagat catacgcacc atgatcatag ttgtaagtca actctaattc
    17041 atagtcatcc ccaggaagtg tcaaataaac aagtgtaaat ttgttttctg gaaaatcacg
    17101 gcggcggctt tcttcaaaac caaatgcctt ttgataaaat tcgattgatg cttcaagatc
    17161 tttcactcga acacatgtgt gagccatttt catagattca tctctccttt tccttttact
    17221 caatcataac aaaaagaagt aaggaaatag ttgatttgtg cttgttcatg agaaatttct
    17281 catgtaaaat ggatagaaaa taaaactgtt aaggtggaaa ctattcatgg ataaaccaat
    17341 ttttggtgaa aaaaagcaat ttgctgatta tcaaacccgt tatgcagcat atattgtcat
    17401 agaaagagat cagcagattg cagtaatcga ggctccaaat ggtgcttttt ttcttcctgg
    17461 cggggaaatt gaaggagaag agacgaaaga agaagcgatt gtccgtgagc tgatagaaga
    17521 aatgggaatt gtggcagaaa tttcttctta tctgggagaa gctgatgagt atttttactc
    17581 gaattatcgt gcaacttatt attataatcc aggatatttc tttgttgctg attcttggaa
    17641 gcgtatagga gaaccaactg aaaagaccaa taaaatttgg tgggtgagcc ctcaagaagc
    17701 gcttgtgaaa ttgaaacgtg gtagccaccg atgggcagtc gagagatgga tcgaaaaaaa
    17761 taaaaactga aagaaagcga gtagatcagc tttctctcag tttttttatt tattacagta
    17821 catatttttt gattgcttca gctacaccgt gctcgtcgtt tgatgaagtg atgacatttg
    17881 ctgcttcttt gacaagagaa acggcatttc ccattgctac tcctagacca gcatattcaa
    17941 tcatggataa gtcgttctcg ttatctccga tagccatgat ttcatcttga tcaataccaa
    18001 ggtgctgtgc gagattcgca actgccgcac ctttacttgc ttctttattt agtacttcga
    18061 agtaataggg cgcacttttg accgttgtgt atttatcacg gaaagtttgc ggtaaacgcg
    18121 caattgctgc atctaaaatt tctggttcgt cgatcatcat cattttgacg atgttcatgt
    18181 ctggtgtcat ttcttcaggt gttcgatatt ttagcggcat cttgactaaa cttgcttcgt
    18241 ggatcgtata agcgctgata tctcgattag gcgtgtagat cgctttgtca tcgatcgaat
    18301 ggagatgact gccgacacgg cgtgccatca cttcgatctc taggaaatct tcatgtgtca
    18361 gtccgtgacg ggcgatgatt ttacctgatt tcgtttgttg tacgagtgcc ccgttataag
    18421 taataacgta atcattatct tgaaaaaggt tcaactcgtt taactgttcc tggacacctg
    18481 gtaagggacg gccagtgcat aaaacgatcg aaacaccttt tgcaacagca gcggcaatcg
    18541 cttcttttac ttcttttgtt acttctcttt tctcatttaa taaagtacca tcgatgtcga
    18601 tagcgactag tttgatagac ataatttcct ccaatatatt ttaatttatt tcaatcaatg
    18661 cgccattacg gatatggctg gcaaattctt gataagtttc gtcaaataaa tcataatgat
    18721 cacgcaagga cggatcaacc atttcttttg gaaagaaaaa tcgctcatct ccttgttctt
    18781 gtcccgctaa tgcagcaaca agctgactaa cctgtgataa ttctatcaaa gttccgtcat
    18841 ttcgcaaaat ctcgatttgt gtgcgatgtc tgccttgttc tgggcgataa aaatcataag
    18901 gtagatcgta gctagaattc acagcagtgt aataaacagg attatatccg accttttcta
    18961 ctaacaaagt gagttcttgg actaattttg aatcccggtt gccagaaaaa gtagcagatt
    19021 tcagtggttt acgattcaaa aaacgtttag ctaaatcatt taaaatagga tcagcagatt
    19081 cagaccattg agtaaagtaa gtgcccaaga ccccatcatc aagtttgagg tattcttcta
    19141 aagtaaagct tccgtcaaga aatggcagaa gtaattgaga atggagttca aaactgctgg
    19201 catcttcttg atacaactcg tgtgcgcgat gaagcagatg ctccaaaatt acttccatac
    19261 cacgggaaac aggatggaag tacacttgca catacatttg atatcggcta acgatataat
    19321 cttccactgc atgcatgcca ctcatggaaa aagcgatccc gccttcataa ggacgaatca
    19381 cacgtaatat tcgggtaaga tcaaatgttc catattctgt tccagtgaaa taagcatccc
    19441 gaagcaaata gtccattcga tctgcatcga tctgactaga aatcatttgg acaacttgtg
    19501 ggttggggta agttttttgg atcacactag ctaccttttc aggaaaacct tcttctactc
    19561 ggttaagaat ctgatacact tctgtttctg gagaagtgat gatctctaca gtgatggctt
    19621 catgattcgt atggaagata tgttcaaatg tatgagagta aggaccatga ccgacatcat
    19681 gcaacagtgc cgcacataaa gtcaccagcc gttcctgatc atcccagcct ccgttgccta
    19741 ttttttcttt tgaaaaattt cgagaaaaga tatcgcagat tcgtcttgaa atctcataaa
    19801 cgcctaatga atgagtaaaa cggctatgtt ctgctccgtg aaaagtgaaa gaagaagtgc
    19861 ctaattgttt aatgcgtcgt agacgttgga cttctttcga attgatcaaa tctaagatta
    19921 cttgatgctg gacatggata tagttatgga ctggatcacg aaaaactttt tccataggca
    19981 gcatttgata tttataaggg gttgtcataa tgtagctcct ttatgttatt acgttgttcc
    20041 attctctgga tttggttgtt ccattgctcc agctggattt ctttttttag ccagtcggta
    20101 gaatcctgta aatcacttgc agaatacagc ttttcaaaag cttttgcaaa tgcttctttg
    20161 acttctttta caaaaagggg ttggtcaagc agttcttcaa gtgttgccat tgtagcagga
    20221 tccactgcag gatagccatt tgtaccaaag tcttctttta aagcagattg ataaaatcgc
    20281 ttcatgactt gcccgcgttt ttcctgatta ccgttgacac ttaagtacat cataacagaa
    20341 atcccatttt tgatccgacg ctgagcgata ccggcaaatt ttttgccatg gatgcttaaa
    20401 tcataagttc caggacaata agaatgggtg atttcgaatg cttgtatcgg tgcctttgga
    20461 aaagcgtatt tagtcaatag gagcatttgc tcgtaagcgt catctatggt tatttttttt
    20521 gtgtcacttt gggggaaaat caaagaaata ttcaaaattc cttcatctga tacgatccct
    20581 aatccacctg aatttctgat cactggagcg taacctgcat ctttcagtgt ttggatcccg
    20641 tcacttagaa aaggagttcg aacatccttc atacctaaga tgactgtgca gtcatattgc
    20701 cagaaatgta aaaagatttg ctggtgttct ttagaatatt ccgttaatat atctgttaat
    20761 gcaaaagggg aaaagtcgtt tttttgataa atgtgctgat ctagtcgaat cgcagggttc
    20821 attcaaccgg ctcctttgta taaattcata ttttctacaa tattatactc taaaatcaaa
    20881 gagttgtttt ggaaaaaagg ttttctgtaa aaatggacaa gttggttgac aaaacaaaag
    20941 aaactaggaa caatgtagct atgagaagta aaggagaatg aataatggaa ttatcacaag
    21001 gaacaccgat ttcaatcaaa ttgcgtacaa aagtaaaaca gcaaggggaa gtgcaagatt
    21061 tttattttga tttgaaaggg caaatggtga caattggcga tacgctgtac atacgttata
    21121 aagagattca ggaggagacg ggcgatgaga ttcctgtaac gatcaaatta gttccagatg
    21181 gtcatgtaca gctgatccgt gcaggagaaa tgcggatgcg tttgaaattt ggctataaag
    21241 aacggttaga aacatcctat cgaacaccat acggtatgat ccagattgct acttttacga
    21301 aagaactgca tgttagtttg aaagaccgtc ccacaggagg gaaggttcga atcgattatg
    21361 atctgttcat gggaccggaa aaaatcggcg aatattattt gaccttggat tttacagcgt
    21421 aaatataaaa gaaaagtttg attttttgtg tctgattttg tatgataagg agtagactgt
    21481 gaaaggacgt gtcctgattg gaaattagtg tatttgaagg tgcaaacaaa agcgaattat
    21541 caatgatcga agtagcacac gcgatcttag aacaacgtgg cgatgtcatg gatttttctg
    21601 atttagctaa ccagatccaa aactatcttg ggaaatcaga cagtgaaatt cgtgattcac
    21661 tcgcacaatt ttatactgac ttgaatattg acggcagctt tatctcatta ggggataatc
    21721 gttggggctt gcgttcatgg tatgctatcg attcgatcga tgaagaagta aaccatggaa
    21781 tggacgaaga ggatgaagat actccacgtc gccgtaaacg caagaaagtc aacgccttta
    21841 tcaatgatga tgaagacgcg atcgattaca atgacgatga cccagaagat acagatctga
    21901 cagaagaaga tgacgatgat ctgtttgatg acgatgatga tgaagatgaa gaaatcgctg
    21961 cttataattc tgacttgcaa gaaatcggag cagatgaccc gacagatgat gaagaagact
    22021 tgccaggaaa tatcgaagaa gatttaagta tcatagacga agacgatgat gatgaagatt
    22081 atgaagagga agaattttct gactctgaaa aagaataaac attattttct tacgagaggt
    22141 tgtgacagag acgatcagcc tatcagaata aggaggtgtg acacgat
//
LOCUS       DARBEC010000045        22031 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.045, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000045 DARBEC010000000
VERSION     DARBEC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22031)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22031)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22031
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.045"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            367..555
                     /locus_tag="SCL80_001573"
     CDS             367..555
                     /locus_tag="SCL80_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107443.1"
                     /translation="MELIVFTNNGQTYHFYEVENFKPTTTGFSFIYTGKATGVTRSAT
                     FNNTSTAGYALAEVQNES"
     gene            545..805
                     /locus_tag="SCL80_001574"
     CDS             545..805
                     /locus_tag="SCL80_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107444.1"
                     /translation="MNHEKFIEKCKAIVRERIENEIADLSGAVPEFSIFIVWSCKTLQ
                     NSKALVSASLKGAPYFEITLNGDKGEIYVDTYLKKSNECIKV"
     gene            846..1073
                     /locus_tag="SCL80_001575"
     CDS             846..1073
                     /locus_tag="SCL80_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107445.1"
                     /translation="MRNYWYVSLTNEYPRTIGDCSVRVVRSVQIKGKYSIVEMLREAT
                     PNEVDKCKLIYCGHGYWKDEYIQYNIERYIK"
     gene            1138..1710
                     /locus_tag="SCL80_001576"
     CDS             1138..1710
                     /locus_tag="SCL80_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107446.1"
                     /translation="MKLKDYIREGYNVVTAPNLAYKIQEDYPNALVFTDRALDAIPIG
                     KLLVDRFYSNNPAVLSAKPLQNAYAIERFTCEFTGYETVVPMTGETGRKVIQQIKKRI
                     AELTLDDGSNTNLLEIKLRDTDSVPEVYYKGQRMDEMPIGLVDITYHWDTEEYECQVP
                     KGANDITIEYYDSHNNKYLDRKIIGHKRDM"
     gene            1711..2022
                     /locus_tag="SCL80_001577"
     CDS             1711..2022
                     /locus_tag="SCL80_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1107447.1"
                     /translation="MIDVSSKQARARFYGSTEWRKLRSQCLERDHYECQWCKAEGRLT
                     TQHDSILEVDHIKELEYHPDLALDINNLRTLCKDCHNKRHNRMKYRGQQKKRKWDDEW
                     W"
     gene            2013..2273
                     /locus_tag="SCL80_001578"
     CDS             2013..2273
                     /locus_tag="SCL80_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107448.1"
                     /translation="MVVRKTANRDDVKRAEAIAMVDKAIDKLVLFKSQLIEGTTTVED
                     ISINNRLIGNANEVTGTFNINVDIDYTLSPFAHIVESNEQDG"
     gene            2434..2793
                     /locus_tag="SCL80_001579"
     CDS             2434..2793
                     /locus_tag="SCL80_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107449.1"
                     /translation="MADLQERNRLVSAEERRLNKLFKDIDKDKKKVVSGLITQAARLK
                     ILLDEMWIDISEKGDYELFSQSENQKPYERERPVAKQYNSRDQAYQRVIKQLTDYLPE
                     EKREPTKNAALDGSDLL"
     gene            2790..4433
                     /locus_tag="SCL80_001580"
     CDS             2790..4433
                     /locus_tag="SCL80_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1107450.1"
                     /translation="MTTLKPFYFDEYVDLYEKGVIPFNKERIQLITYLKNEVLVRDDI
                     YFDEEMIQKFIKYTEKNFFPLAKYQKFITPFIFCYQKEDDEVFFDEILNSIARGGGKN
                     GFMSARDSFFISPLYGVRNYDVTITANSEKQGKVSFKEVYEMVQMRKLEKQFYLTKMA
                     IVNNVTNSIFSYRTNNPKTMDSARDGCLEFDEIHQFESSDLVDIQRSGLGKIKHPRTF
                     YNGTNGHVREGFYDKMLERAQKIFSGENKNDRLFPFICKLDNIDEMDNPKMWSKANPM
                     FEEDSAYSKRLFSTVMKEYKKLEDEPSGRREFVVKRMNFTEGDAESDITTHEKLMATK
                     QELPNLNGRSCVAGFDYASIRDFATVGLLFKIDDKFIWQQHSFARKKFLDTFKLKAPI
                     KDWEKKGLVTIVDEPSIDPRHLIDWLNEKRKIHQIEIVCADGFRMDLLKPLLEKESYN
                     FEFIRNIRGVQSKVAPIIEDGFANERFIFGDDPMMRWYTNNSFIKEDKLGNKTFLKKE
                     PVRRKTDGFHAFLAALYKREEITDMDYEEAFDMLDEIEF"
     gene            4468..5550
                     /locus_tag="SCL80_001581"
     CDS             4468..5550
                     /locus_tag="SCL80_001581"
                     /inference="COORDINATES: protein motif:HMM:NF028182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107451.1"
                     /translation="MKKRYFLFVTIFIIIAICVLTVFLPQITEYLNYRIFTTKVDDSV
                     TSLSRLEFIKICASVFGPILTVMVFVNTLNMQKKTEQKQNELEVKQAKAENERAKKEL
                     LTQIDREFYFLLNMFIEIQKDQNAKQAVIALQKLAIFNEKGFNQDYAFTSKDLSDSEI
                     GSTIEQSGPLKGTVEYIKFETFDVSKSMGNYQTKTKDVATSLHDQKVAILNEQYEKMQ
                     IYLGRYFKMFHRIVKTLNEYYDDYNDFDVKRYTKYIGTLRTQISPAEFQVILFNSLYI
                     KRGFGLGIQLIGSGFFGDDFDFETNQHFETSINEQWFLSLSTVDSDNSIKRQKLSEYI
                     KELGSVEYKKIANFESLYKLFNETKL"
     gene            5594..6766
                     /locus_tag="SCL80_001582"
     CDS             5594..6766
                     /locus_tag="SCL80_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1107452.1"
                     /translation="MSLFDIFKQSIRNEEPSEWIPDFVYGEEVSARAYLKIMAKNTVL
                     DFVARTMSTLEVKFKNKDGTSDWDYILNVRPNSDMSAATFWQKFFYRLLDDNEVLVIF
                     TDDNQLLIADDFSRTEYAVYDDVFTNVMVKNFVFERSWNMSDVIYMEYNNEELERFTR
                     GLFEDYAALFGRILEVAMRNNQIRGSVSIDSTGTANDEKGKDGKTRSQRLQEYIDRIW
                     NSFKTNSVAIVPKVKGFEYEEYTNKQGVSNQSLDELNKMKSSLIDDVANAIGVPTALI
                     YGEKAELDSNIKAFRKLCIAPLVKKLQDELVAKIISRQEYKNGERIEVKKVLQKDLLE
                     YATQIDKITSGGAFYIDEVRDEVDYDELPNNEGKIRIRTKNYEETVKGGENDNAES"
     gene            6753..7457
                     /locus_tag="SCL80_001583"
     CDS             6753..7457
                     /locus_tag="SCL80_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1107453.1"
                     /translation="MPKAKKVPFQFTNEIQNGKHILTLSGNVQKKYWRDDDVINAKDI
                     RDSLDTVTDDIVIKLNSPGGDVFEGIEIYNYLKDHPSNITVEVTGLAASAATFIVAGA
                     NDVIMNVGTSLMIHEASTFAWGNKQDIQKTLNALETIDDSILAIYSDKTGQSVDKLRE
                     WMNEEKWFTADEAVKYGFANSVKREEPQNEPQNIAAMIQDAVAVAIANLKEPVTNKIE
                     QESKQKSLIARLRKGE"
     gene            7462..8622
                     /locus_tag="SCL80_001584"
     CDS             7462..8622
                     /locus_tag="SCL80_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1107454.1"
                     /translation="MLKITDKTADAKKAFNAISSKEDATPEQINAALEEYVTAIAEDA
                     GKQVRAEYEELKNVTDNRVLEARGIPTLTAEETKFYNEAVKSGGFDSDVVWPETILER
                     VFENLQNDHPILGLINFTPTVGRVKVIRARRKGVAVFGPLHKDLEGQLDAEFGATEFV
                     QLALTAFFLISNDTLDLGPRWIDRFINLSLSEAVRDVWAKKIITGSGKDEPIGLLKDL
                     DGAVTSGVYPDKASAGTLTFAKEKIVLELAGVMKKLSKYTYKVDGDDEGEVKYRSVAG
                     RVYLIVNPVDYWDIVARVTFANLNNVYGTTMPFIAVDHIIESVDVPESKCIAYVDKEY
                     EATQSRAEKVYVYKETFAMKRATLYAVDMLGNGYPTNNDAAQVYDLDFTAGE"
     gene            8698..8976
                     /locus_tag="SCL80_001585"
     CDS             8698..8976
                     /locus_tag="SCL80_001585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEG1107455.1"
                     /translation="MNTEEFIKEYKARFRIFHTSEDEDIGKQLESGFADIKSLIGSFD
                     PSTYEKGKELVYERTRYLRNESLEYFYDNFQTMIMDASIDLAGAGYAN"
     gene            8957..9328
                     /locus_tag="SCL80_001586"
     CDS             8957..9328
                     /locus_tag="SCL80_001586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail adapter protein"
                     /protein_id="HEG1107456.1"
                     /translation="MLDMPIKSNYKKPKIVAGDLNTPVTFFEFQPNKGPEPGDEEKKE
                     LYFCTALVYNPSMKDRDILGTKEGVTIKIRDPHQSYIPTNKHKVRILDYRYGDKVWEI
                     IDVSPDLEENSIVKIVLELIT"
     gene            9344..9748
                     /locus_tag="SCL80_001587"
     CDS             9344..9748
                     /locus_tag="SCL80_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107457.1"
                     /translation="MSNVKFEGVQETIQALEKKYGEKKTKTLTKKAINVGAEKVEKQL
                     QTDMTVFKDKGYTIAEVVRKNATYRDYKAEAEIGWNGPHQRYRIIHLNEWGYTRKGKQ
                     IRPRGFGVITKSLKNSELLYFDAVGEEVKKSL"
     gene            9745..10155
                     /locus_tag="SCL80_001588"
     CDS             9745..10155
                     /locus_tag="SCL80_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107458.1"
                     /translation="MKDMLNIIYEALCLNEYIHSMTYNENTEQYRIKFYEQPETADKT
                     GPMITIRPVDTPNEAYHGSNKELSVEHMIQIDVESKYRATCKQIQYEIKKEMKKLGFG
                     QISGQGLDEYFTETNRFVDARRYEGNTKIYDTEY"
     gene            10216..10803
                     /locus_tag="SCL80_001589"
     CDS             10216..10803
                     /locus_tag="SCL80_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1107459.1"
                     /translation="MTLVGFKKMTIGVFDSTGKIPAANLYVIEGEQNKGATVSAEISG
                     LSKEPSKVYGSNIAYYVSQKGTGDVSATFGLLDLPTEVNDKILGYKTDTNKISFLGED
                     TEPPYCAILMESEDLNGDTAMLAMFKGKFSRESINLNTTTNEAFEPEAEEYVFSAIAN
                     DVEGDAKGQTVAKYVGDEEASITGLKEMVFPAAGE"
     gene            10876..11343
                     /locus_tag="SCL80_001590"
     CDS             10876..11343
                     /locus_tag="SCL80_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig domain-containing protein"
                     /protein_id="HEG1107460.1"
                     /translation="MPDTFKIYKKNGTEFTKVAEGESPLSITGIAANTQVAKGDYQAT
                     RVVGGQESVKVDIPAFTTLPISVTGVTLDKTTAEVEQGGTLKLTPTVTPANATDKTGS
                     WSSSNTAVATVTGGNVTVKTDAEVGGTTEVSFTTTDGGKVAKCTITVIEKAAG"
     gene            11389..11697
                     /locus_tag="SCL80_001591"
     CDS             11389..11697
                     /locus_tag="SCL80_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107461.1"
                     /translation="MAKVRIELKEESGKKVYENIDTTGKDYRKALETIKKLNEDGVMI
                     WNRLDIYLDFAVGIFRDSKLTAEQILDGLPSEKVMSTLDDILGEVMGIESNPDPDAKK
                     "
     gene            11892..18626
                     /locus_tag="SCL80_001592"
     CDS             11892..18626
                     /locus_tag="SCL80_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1107462.1"
                     /translation="MANGKPLGNMIIKLDLDSSAFSKGLAGAKNAVNHQMKAMKSQMQ
                     VMNSSGNSLGALQAKYNGLGGVLQANEKQVELLTKAYKDSFDSNGNATASTAKYANQL
                     NQATARSASYESQMKTTVGQIARMKVETEGFTGKLKANSDVLINSGKKIEAFGKGVSG
                     IGSALTVGVTAPIVAGATAVTSAAISWESAFAGVKKTNDEVVDSNGNVVYSYSDLEKG
                     LRNLATQLPGSHQEIANVAEAAGQLGIKTENVVSFTKTMIDMGESTNMSAETAATSLA
                     RFANITQMSQKDFDKLGSVIVDLGNNFATTESEITEMGLRLAGAGKQIGMSQGEILGF
                     ATALSSVGVEAEAGGSAFSKVMIQMQLAVEKGFGAFDQLKQMAEEQGVPWVNLVNAVR
                     DGGKSLKAVSEQMGFTSSDLKKMYKEADNSKSSLENFANVAGVTSEQFQKLFKSDPSE
                     AIIKFIQGLKDSEKHGTSAIKVLDDMDIKEVRLRDSLLRAANASDIFKGAIEKGNGAW
                     KQNTALTNEANKRYETTESKLKILKNEVIDTAIDLGGPFVDALRDGLNVSKPLIKNLG
                     NMAKAFSAMSDDQQRNIIKWVGLAAAAGPALKLLGTGTSVIGKVTSTTGRLTKSLVDL
                     AAKAAEKKAMDAFASSVTTAGAAAAKTAGAGGIGGLVPMMGKTASAASTAAGASGIGA
                     MTTSLGLLGPALLGIVGVGGALAVGYGAWKLFGEEAWNSSQRVKQWGTDVGEEIDSTL
                     DGVQKNIEGANGQFGLLKEGFTVDDANNMAANFATAGTSLETSLTGRIKALDDALKGL
                     PQSVQDAMKEVAESEEETLGQSLSKIKDNNEEIKKIRERAANQNRDLTVSELQQIQDL
                     MKGTSQAYVDTLDVSAKDRKKILSAMNGDVAQATEEEAKTWLQSLAKQKAATTENYRQ
                     MLEEKKAALKEKGVSKEFIDALEDEMNDYIKTTNQGFDSQIAAITNKYPQLINEVSLS
                     NGQLFSKTAAAMDNTGQYTKVFLESNEKILAQAKKTSDEIADNAEKNAEQLGMVADEA
                     THAGSTWNGLVLDEKTGKVKTNVQEVVNEATKDFTKWNELKPVVHDAKLTTNAKDVIS
                     IAAVQNGYWDDMEWKEKEALLEDECSKNVYKALENAGKWNELDIPSKEAILTSNTPEV
                     MGETLMNLGLWDTYKLQVKDLDLNAYKVYDAIDGSEEKLNSWAQLDNPTKELLLDNSD
                     FSTKLFNSEEMFKRWNQLSSNAKFLSANNTKFTEVLLSSDRMWNQWVNLPDNEKRLLA
                     DNEDLATTVLSSEEIYGRWTQLPDNQKYMLADNTDLANKIFASEESWNAWKAIPDTVK
                     NLLGNNIDLLAKVKDGTVSVEEYNKVMPLLKTLYGDNVNLKEAISGAKSSIENYNKNI
                     FPSDKTAVGHDEASQAAKDAFEAFNIFQTKIPDKITKTVSADFIGPMPKNAKGTNFHP
                     GGAAMVNDQKGPTYEELITLPSGETFIPKGRNVVLDLPRGSKVLNATKTKRLVPKYAD
                     GIGNITTVSSTLNLDALILAINELTTVLRTQLMAGTTDAKDTKLSDETKVADPIIPDS
                     LSEKSDQYLGIGAQWLTNLMNGWNSAVPQYMNSEMVFITNYLNALKLQNNPNYTQGAT
                     WNKNLLNGWNSLTGTFIATINSFCNQAMVTLRNYNTPMYNNGRTWQQNNLNGWNSLYG
                     SFIARVNQLGNDSINNLRSKSGGFYNAGTFLLQSLINGMNSLGNSLSTTMNNVANTMV
                     GGIGKGVNGVISGVNYVLKEVESSKNIGNWPIPQYAKGTDGHPGGLAMINDQKGLVHE
                     EYVQMPDGRSFIAKGRDLLVNLPKGAQVLNASLTKKLKERLNIPHYEKGIGNLDIVDL
                     LDDEKRMLEFLTSKVDFSGINEPWLDMTKSGTSLMSKAANTMLQSKLSEFFTHGNFDG
                     AVNANGVYQYLVDVAQKVMGKFPGLTVTSGYRAGDAYYHGKRQAIDLAYPGISGDPRY
                     TAAANYAFEKFPSKIAYVITNGRVRDRMGLSGTRSSGQWTNWPDGDHFDHIHLNGSMG
                     SGDIFNGGVGGSGVARWRSYVSKALKMNGLPATAAYINAWMSQIQTESGGNEKAIGGN
                     DGLAEGNATGLLQTKPGTFAANAFPGHGNIMNGFDNMLAAINYAKKRYGVAGMLQVIG
                     KGHGYANGGLITKDGLYRAGEGNKPEMVIPLTRKTRAIELMGQALAFLSGSNKKETKS
                     EINSVDNRHILQAIEKQSRMTNDLLSLLLSFFKSNNRSDKELALDIQKILIRRT"
     gene            18630..19502
                     /locus_tag="SCL80_001593"
     CDS             18630..19502
                     /locus_tag="SCL80_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate protein"
                     /protein_id="HEG1107463.1"
                     /translation="MRTVILRNKLNEEIDLSTEDYFATEMSNLGFEVTKEHIGQWGSF
                     REIGESIELSEFQSSVMISVHGFREQQLYGSLVEFLANGPFELEFSFDSETMIRKCSL
                     KSLSKTEIDSQSSLLTDTLNLYFTSSWYSIKREKLIQNSTSLNTRGKVYSFVRPYVYT
                     KNVWEKKGVFKFSNNSVYLTNSTERMSPLKIRIIGECSNPYWEVIQNSKVVASDGYFI
                     NMKDTQILEVSSLLEDQTAILKDVGGVESSVYQQQDFTKSNFVQAPLGEFSVVFHVGH
                     ANVEIELYEERDLF"
     gene            19499..20548
                     /locus_tag="SCL80_001594"
     CDS             19499..20548
                     /locus_tag="SCL80_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107464.1"
                     /translation="MILAITLFHRDMNLYNERLISTEFEFGTDEIGEEVSNFITDKYV
                     HVKSGDFLLAKFIPSGKIAYFGVISSQEDKKINCKGLLSLADSEIPTVKISGANYEEH
                     IRRLIEYYLLNDPTKQLKNILEVKTESVTSHSYQAVDTNKRRLNAYIINGFKKYNVKW
                     YVKEIKGKKIYTGIRAVDDSIYIKDNSSEFNDWDVFVQSPGPGNENKLLIVDKAMKDI
                     ENPLVLSTWYLDDENNLTQDSTNKNIALPTVNLVNIYDQTQEDRPSYEEVAKSELKGN
                     AYSHEIKVNVVLNARNINVENIETGMLATIVYKEQTYKSVLTAWRISSSKKNIELTFG
                     NVRSRFMDYFEDNGG"
     gene            20552..21376
                     /locus_tag="SCL80_001595"
     CDS             20552..21376
                     /locus_tag="SCL80_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107465.1"
                     /translation="MVSNVDGYQFENVKVSAENDARLYHVLYNRKNQVIDGYDQSMNL
                     SSSGLTVKVAAGAAIIQGRMVVVRQEESITVPANSSGYIALTVDLTQEVIPGSILPES
                     EEYEWTNNQVKLEFITKVIKGNLNNGDKVYNLPLCSVNSTGSTVSISKISDSYELTLS
                     KGEILWRGTALMHDTQTVQPSKKIWQTVSGFLLVWLPYENGQAIEDRYVTTPFYKERI
                     TFTNVLGEIVSGFDDYHKKWFSKRINYNSNTNIFTGAASNASGDNANMVLRYIVSF"
     gene            21394..>22031
                     /locus_tag="SCL80_001596"
     CDS             21394..>22031
                     /locus_tag="SCL80_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEG1107466.1"
                     /translation="MANLEIKLSANKRQPYLRHRVVGRVGDGGLTTINVQLLEEDEIT
                     PFVINLNGTLKFVGEVSNGNYTEGEPEIIDSTNGLISYTFTKSNFSTSHQFKQAYFEY
                     VDPNGKKVTFQNFIIDVLERVDINSEQAKYYISSLEKLQSEMQTTFNQFISDKQVQYD
                     QIYSKYNELVRLINESDKQVNDRIDKANNRIDQTNQQIGDLGKLKKMYSNSI"
BASE COUNT         7878 a         3297 c         4671 g         6185 t
ORIGIN      
        1 cttctaaatt gttcttatta tctatcatca gttgctgtct attgtcatta tgtcactgtg
       61 gtggaaaggt gtatcgctca tctaaaattt aggtgcaaac tgcaaggttc gattccttgc
      121 cagtgacttt ggtaaccgag gcatcggcgg tttaaaaata taggggtgcg caatttcgta
      181 cgcgttttgt gcatcttgta ggttgctatt acataattgg ttgggttaga ttgagttttg
      241 ggattcggta caaatgaatc gtcaaatgac tcaagcacag gatcggaaac gtccctgcct
      301 gtgcattaca tattagatca ctctttgagt ggtcttttta ttttaaaaaa gagaggattt
      361 tgaaaaatgg aattgattgt atttactaat aacggacaaa catatcattt ttatgaagtg
      421 gagaatttta agccaacaac aacaggattt agttttattt atactgggaa ggctacggga
      481 gttactcgtt cagctacgtt taacaataca tcaacagctg gttatgcgct tgcggaggta
      541 caaaatgaat catgagaagt ttatcgaaaa atgcaaggct attgtacgtg aaagaattga
      601 aaatgagatt gctgacctaa gtggagcagt gcctgagttt agtattttca tagtctggtc
      661 atgcaaaaca ctgcaaaata gcaaagcatt agtcagcgct agcttaaaag gagcaccgta
      721 ttttgaaatt acgttgaacg gggacaaagg tgaaatctat gtagatactt atctcaaaaa
      781 atcaaatgaa tgtatcaaag tctagcaggt gctaggcttt ttctttacat aaaggaggct
      841 gcataatgag aaactactgg tatgtatcac taactaatga atatcctcga accattggtg
      901 attgttcagt gcgtgttgtg cgttctgtac aaatcaaagg gaagtactct attgtcgaaa
      961 tgctaagaga agctacacca aacgaagtgg ataaatgcaa gctgatatat tgcggtcatg
     1021 gctattggaa agacgagtat atccaataca acattgagag gtacatcaaa tgaatgataa
     1081 ctatgattac atcaagttgt tagcaagtaa ttattagatg tttttaggag gaaaactatg
     1141 aaattaaaag attatatcag agaagggtat aacgttgtaa ctgcacccaa tctagcttac
     1201 aaaattcaag aagattatcc caatgcttta gtgtttactg atagagcttt agatgccatt
     1261 cctataggga agttattagt agatcgtttt tatagcaata atccagctgt tcttagtgct
     1321 aagccacttc aaaacgctta tgctattgaa cgctttactt gcgagtttac tggatatgag
     1381 acggtagtac caatgacagg tgagacagga agaaaagtaa ttcaacagat caaaaagagg
     1441 attgctgaat tgacgttaga tgatggaagt aatacgaacc ttctagaaat caaactaaga
     1501 gatactgact cagtaccaga agtttattat aaaggtcaac gaatggatga gatgccgata
     1561 ggtcttgtcg atattactta tcattgggat acggaagaat atgaatgcca agtaccaaaa
     1621 ggagctaacg acatcactat tgaatattat gattcacata ataacaagta cttagataga
     1681 aaaatcattg ggcacaagag agatatgtaa atgattgatg tttcaagtaa acaagcaaga
     1741 gctaggttct atggatcaac tgagtggagg aaacttagaa gtcaatgctt agagcgagat
     1801 cattatgagt gccaatggtg taaagcagag ggtagactaa caactcaaca tgattcaatt
     1861 cttgaagtgg atcatataaa ggagctggag tatcatccgg atttagctct agatataaac
     1921 aacctaagaa ccttatgcaa agattgccat aacaaaaggc acaaccgtat gaaatatcgt
     1981 ggccaacaaa agaaaagaaa gtgggatgat gaatggtggt aagaaaaaca gcaaacagag
     2041 atgatgtcaa acgagcagaa gcaatcgcta tggttgataa ggctattgat aagttggttc
     2101 tatttaagag tcagctcatt gaaggaacaa caactgtaga ggatatatct atcaacaata
     2161 gactaatagg taacgctaat gaagtaacag ggacatttaa tattaatgta gacattgatt
     2221 ataccttgag tccatttgca catattgtag aaagcaacga gcaagacggc tgataaatca
     2281 gtataaagcg aagatatata gagtgaaata cccccggtca aaagattttg agaagaaata
     2341 aaatttttgg gaaccggtgg atggggtcaa ctctgcaaat aaatgatgct attttcacac
     2401 gaccccctcc ccacgtagaa aggaagtgag ttgatggctg atctgcaaga gagaaatagg
     2461 cttgtttctg ctgaagaaag gcgtctaaat aaattgttta aggatattga taaggataaa
     2521 aagaaggtcg tttctggttt gattacgcaa gcggcacgct taaaaattct tcttgatgaa
     2581 atgtggattg atatttccga aaaaggtgat tatgagcttt tctctcagtc agaaaatcaa
     2641 aaaccttacg agcgggaacg accagttgct aaacagtata attcgagaga ccaagcatat
     2701 caacgtgtaa ttaagcaact tactgactat ttgccagaag aaaagcgcga acctacaaaa
     2761 aatgcagcgc tagatggtag tgatttgctg tgacaacatt aaagccattc tattttgatg
     2821 aatacgtaga tttatacgaa aaaggggtaa tcccttttaa taaagaaaga attcagttaa
     2881 ttacttattt aaaaaacgaa gtgctagttc gggatgatat ctattttgat gaagaaatga
     2941 ttcaaaagtt tatcaagtac actgaaaaaa atttttttcc tttagctaaa tatcaaaaat
     3001 ttatcacacc tttcattttc tgctatcaaa aagaggacga cgaggtgttt tttgatgaaa
     3061 ttctaaatag tattgcgcga ggcggcggga aaaatgggtt catgtcagca agagactcat
     3121 tttttattag ccctctctac ggtgtcagaa attatgatgt aacgattaca gcgaattctg
     3181 agaagcaggg aaaggtatct tttaaagaag tttatgaaat ggtacaaatg cgcaaactgg
     3241 aaaaacaatt ttatttgact aagatggcta tagtcaacaa tgtaactaat tcgattttta
     3301 gttataggac aaacaatcct aagactatgg atagtgcaag agatggctgc ttagaatttg
     3361 atgaaatcca tcagtttgaa tcttcagacc tagttgatat tcagcgtagt gggctaggga
     3421 aaataaaaca tcctcgaact ttttataatg gtacaaatgg tcatgttaga gaaggttttt
     3481 atgacaaaat gttggaacga gcacaaaaga tttttagtgg agagaacaag aatgatcgct
     3541 tgtttccctt tatttgtaaa ctggataaca ttgatgaaat ggataatcct aaaatgtggt
     3601 caaaggctaa tccaatgttc gaagaagatt cagcatattc aaaacgtctg ttttctacag
     3661 tgatgaaaga atataaaaaa ctagaagatg agccatcagg taggcgtgaa tttgttgtta
     3721 aacgaatgaa ttttacagaa ggtgatgcag aatcagatat tacgactcat gaaaaattaa
     3781 tggctactaa acaagaattg ccaaatttga atggtcgttc ttgtgtggct ggatttgatt
     3841 atgcaagtat tcgtgatttt gcaacagtgg gtttgctttt taaaattgat gataaattta
     3901 tctggcagca acatagtttt gctaggaaaa agtttctaga tacattcaaa ctgaaagcgc
     3961 ctattaagga ttgggagaaa aaaggtttag tgacaatcgt tgatgagcca tcaattgatc
     4021 ctcgtcattt aattgattgg ttaaatgaaa aaagaaaaat ccatcaaata gaaatcgttt
     4081 gtgccgacgg atttcgtatg gatttgctga aacctcttct agagaaagaa tcatataatt
     4141 ttgagtttat cagaaatata agaggtgtgc agtcaaaagt tgctcctatt attgaagatg
     4201 gttttgcaaa tgaacggttt atttttggtg atgatcctat gatgcgttgg tatacaaata
     4261 attcgtttat caaagaagac aaattaggga ataaaacatt tttgaaaaaa gagccagtaa
     4321 ggcgtaaaac agatgggttt catgcttttt tggcagcttt atataaacga gaagaaataa
     4381 ctgatatgga ttatgaggaa gcatttgata tgttggatga gattgaattt taaggtatac
     4441 tccttattga aaggggtagt tatagaagtg aaaaagagat attttctatt tgttacaata
     4501 tttataataa tagcaatttg tgttttgaca gtatttttac cacaaattac tgagtattta
     4561 aattatcgga tatttaccac aaaagtagat gatagcgtta ctagtttaag taggctagaa
     4621 tttataaaaa tatgtgcatc tgtgtttggt cctattttga ctgtaatggt atttgtgaat
     4681 actttaaata tgcagaaaaa aacagagcaa aagcaaaatg agcttgaagt aaagcaagct
     4741 aaggctgaga acgagagagc aaaaaaagag ctgttaacac agatagatcg tgagttctat
     4801 tttttattaa atatgtttat tgaaatacaa aaagatcaaa atgccaaaca agctgttatt
     4861 gctttacaga agttggcaat ttttaacgaa aagggtttta atcaagacta tgcatttact
     4921 tctaaagatt tatcagatag tgaaattggg agtacaattg aacaatcagg accgctgaag
     4981 ggcacagttg aatatataaa atttgaaaca tttgatgtaa gcaagtcgat ggggaattat
     5041 caaacaaaaa caaaagacgt tgctacaagt ttacacgatc agaaagtagc tatattgaat
     5101 gaacaatatg aaaagatgca aatatatctt ggacggtact tcaaaatgtt tcaccgaata
     5161 gttaaaacat taaatgagta ttatgacgat tataatgatt ttgatgtgaa gaggtataca
     5221 aaatatatag ggacattaag aacacaaatt tctccagctg aattccaagt gattttattc
     5281 aattctctat atataaaacg aggttttggc ttaggaattc aacttattgg aagtggtttt
     5341 tttggagatg attttgattt tgaaactaat caacattttg aaacaagtat caatgaacag
     5401 tggtttctat cactatcaac tgttgacagt gataactcta taaagcgaca aaaattatct
     5461 gaatatatta aagaattagg atccgtagaa tataaaaaaa tagctaattt tgaaagttta
     5521 tataaactct ttaatgaaac taagctctag aaatagggct tttttatttt tctttgaaag
     5581 gtggtgaaaa catgtgagct tatttgatat ctttaagcag tcaataagaa atgaagagcc
     5641 ttctgagtgg ataccggatt ttgtatatgg agaggaagta tctgctcggg catacctcaa
     5701 aattatggca aaaaatactg ttttagattt tgttgcaaga actatgtcaa cgttagaagt
     5761 taagtttaaa aataaagatg ggacatcaga ctgggattat attctaaatg ttcgacctaa
     5821 ttctgatatg tcagcagcta cattttggca aaaatttttt tatcggctct tagatgacaa
     5881 cgaagtgtta gtaatattta cagatgataa tcaattgctg attgctgatg attttagtcg
     5941 aacggagtat gccgtgtatg atgatgtttt tacaaatgtg atggtaaaaa attttgtctt
     6001 tgaacgttca tggaacatgt ctgacgtcat ttatatggaa tataataacg aagaattaga
     6061 aaggtttaca cgaggtttat ttgaagatta cgccgcgttg ttcggacgga ttctagaagt
     6121 tgcaatgcgt aacaaccaaa ttcgtgggtc tgtatccatt gattcaacgg gaacggcgaa
     6181 tgatgaaaaa ggaaaagatg gtaagactag atctcaaaga ttacaagaat atattgatag
     6241 gatttggaac tcttttaaaa ctaactcagt tgcaattgtt cccaaagtga aaggctttga
     6301 gtatgaggaa tacaccaata aacaaggcgt atcgaatcaa tcgctagatg aactgaataa
     6361 gatgaagtcg tctttaattg atgatgttgc caacgccata ggagtaccta cggcgcttat
     6421 ttatggtgaa aaagctgaac tggattcaaa tattaaagct tttcgtaaat tgtgtattgc
     6481 tccactagtt aaaaaacttc aagacgaatt ggtggcaaaa ataattagtc gtcaagagta
     6541 caaaaacggg gaacgtatag aagttaaaaa agttctccaa aaagatctat tagagtatgc
     6601 tactcaaatt gataaaataa catctggtgg tgcgttttat attgacgaag ttcgtgatga
     6661 agttgattat gatgagttgc caaataatga aggaaaaatt cgtattcgta cgaaaaacta
     6721 tgaagaaact gtgaagggag gtgagaacga caatgccgaa agctaaaaaa gtaccatttc
     6781 agtttactaa cgaaattcag aatggtaagc acattctcac cttgagtgga aatgtacaga
     6841 aaaaatattg gcgtgatgat gatgtcataa acgctaaaga tattcgtgac tctctagata
     6901 ctgtaaccga tgatattgta atcaagttga acagcccagg cggtgatgtg tttgaaggga
     6961 ttgagattta caattattta aaagatcacc catcaaatat tactgtagaa gttacagggc
     7021 ttgccgcaag cgcagcaacg tttattgtcg ctggtgccaa tgatgtgatt atgaatgttg
     7081 ggacttcttt gatgattcat gaagcttcta catttgcttg ggggaataaa caggacatcc
     7141 aaaaaacgtt aaacgcattg gaaacgatcg atgattctat cttagctatt tattcagata
     7201 agactggcca atctgttgat aagttgcgtg aatggatgaa tgaagaaaaa tggtttacag
     7261 cagatgaagc tgtcaaatat ggatttgcca attctgtgaa gcgtgaagaa ccacaaaatg
     7321 aacctcaaaa tattgcagcg atgattcaag atgctgttgc cgttgcaata gcgaatttaa
     7381 aggaacctgt tactaataaa atcgaacaag aatcaaaaca aaaatcatta atcgcacgat
     7441 tgcgaaaagg agaataaaga tatgttaaaa attacagata aaactgcaga tgcaaaaaag
     7501 gcatttaatg ctatttcttc gaaagaagat gcaacacccg aacaaattaa tgctgctcta
     7561 gaagaatatg taaccgccat tgcagaagat gccggcaaac aggtgcgtgc tgaatatgaa
     7621 gaattgaaaa atgtgacgga caaccgtgta ttagaggctc gtggaattcc tacactaaca
     7681 gcagaagaaa caaagtttta taatgaagcc gtaaaatctg gaggtttcga ttctgacgta
     7741 gtttggccag aaacgatttt ggaacgtgtt tttgaaaatc ttcaaaacga tcatcctatt
     7801 ttaggcctca ttaactttac accaactgta ggtcgtgtca aagtaattag agcacgtcgt
     7861 aaaggtgttg ctgtttttgg accattgcat aaagaccttg aaggccaact agatgctgaa
     7921 tttggtgcaa ctgaatttgt tcaacttgcg ttgaccgcat ttttcttaat ttctaatgat
     7981 accttagatt taggaccacg atggattgac cgttttatca atctttcttt aagtgaagca
     8041 gtacgtgatg tttgggctaa aaaaattatt acaggttctg gtaaagatga gccaattgga
     8101 ttgcttaaag atctagatgg tgcggtcaca agtggtgtat atccagataa agctagcgca
     8161 gggacattaa cttttgcgaa agagaagatt gttcttgaac ttgcaggggt gatgaaaaaa
     8221 ttatctaaat acacatacaa agtagatggt gacgatgaag gtgaagtgaa ataccgttct
     8281 gttgctggac gagtgtatct aattgttaat cctgttgatt actgggatat cgtagctaga
     8341 gttacatttg caaatttaaa caatgtgtat gggactacga tgccatttat cgctgttgat
     8401 catatcatcg aatctgtaga cgtaccagaa agtaaatgta ttgcatacgt tgataaagag
     8461 tatgaagcta ctcaatcaag agctgaaaaa gtctatgtat ataaagaaac ttttgcaatg
     8521 aaacgcgcaa ctttgtatgc tgtcgacatg cttggtaatg gctatccaac aaataatgat
     8581 gcagcacaag tatatgactt ggactttaca gcgggggagt aatgtcccct gcaattggtg
     8641 agataacacc gacgactgat ggggcagtca ttaatctttc gtaaatgggg ttagagtatg
     8701 aacactgaag aatttataaa agaatataag gcccgatttc gtattttcca tacttccgaa
     8761 gatgaggata tcgggaaaca gttagagagc gggtttgccg atataaaatc tctaatcggc
     8821 agttttgatc cttccactta tgagaaagga aaagaattag tttatgaacg tactcggtat
     8881 ctacggaacg aatcactaga gtacttttac gacaattttc aaacaatgat tatggatgca
     8941 tcaattgatc tggcaggtgc tggatatgcc aattaaatca aattataaga agcctaaaat
     9001 tgtagctggt gatctaaata cgccagttac tttttttgag tttcaaccta ataagggtcc
     9061 agaacctggt gatgaagaaa aaaaggaact gtatttttgt acagcactag tttacaaccc
     9121 atcaatgaaa gatcgtgaca ttcttggcac aaaagaaggc gttacgataa aaatacggga
     9181 tccccatcaa tcttatattc ctactaacaa acataaagta aggatattag attatcgata
     9241 tggagataaa gtttgggaaa taatcgatgt ctcaccggat ttggaagaaa attcaattgt
     9301 aaagatagta ttagaattaa ttacttaaat ttggtgataa atgatgagta acgtaaagtt
     9361 tgaaggcgtt caagaaacaa tccaagcttt agaaaaaaag tatggagaaa agaaaactaa
     9421 gacattaaca aaaaaagcaa tcaatgttgg cgctgaaaaa gtagaaaaac agttgcaaac
     9481 ggacatgacc gtttttaaag acaaaggtta tacgatcgct gaggttgttc gcaagaacgc
     9541 tacgtataga gattacaagg cagaagctga aatcggttgg aatggtccac atcaacgata
     9601 tagaatcatt catttgaatg aatggggata tacacgaaag ggcaaacaaa ttagaccgag
     9661 aggatttggt gtaataacaa aatctcttaa gaattccgag ctgttgtact ttgatgctgt
     9721 aggagaggag gttaaaaaga gtttatgaaa gatatgctta atatcattta cgaggctctt
     9781 tgtttgaatg agtatattca ttcaatgacc tacaacgaga ataccgagca atatcgtatt
     9841 aaattttatg agcaacctga aaccgccgat aaaacaggtc caatgatcac aattcgtcct
     9901 gtagatacac ctaacgaagc gtaccacgga agtaacaaag agctttctgt ggagcatatg
     9961 atacagattg acgttgaatc gaaatataga gcaacatgca agcagattca atatgagata
    10021 aaaaaagaga tgaaaaaact aggctttggc caaatttccg gtcaagggtt agatgaatac
    10081 tttactgaaa caaaccgctt tgtagacgcg cgtcgttacg aaggaaatac aaaaatttac
    10141 gataccgaat attagaaatt aagacacgaa aataacgtgt ctttttttat tacccaaaat
    10201 taggaggaaa attttatgac tttagtagga ttcaaaaaaa tgacaatcgg ggtctttgac
    10261 agcacaggga agattccagc agcaaattta tatgtaattg aaggtgaaca aaacaaaggg
    10321 gcgactgtat ctgctgaaat cagtgggcta tcaaaagaac catcaaaagt ttacggatcg
    10381 aacatcgctt actatgtctc tcaaaaagga acaggcgatg tttcagctac ttttggattg
    10441 ttagatttac caacagaagt aaatgacaaa attcttggtt acaaaaccga tacaaacaaa
    10501 atcagcttct tgggagaaga tactgagcca ccatattgtg caattttgat ggaatcggag
    10561 gatttgaacg gcgatacagc aatgcttgcg atgttcaaag gaaaattcag tcgtgaatct
    10621 attaatctta acactacgac aaatgaagct ttcgaaccag aagcagaaga atatgtattt
    10681 tcagcgattg ccaatgatgt tgaaggtgat gctaaaggcc aaacagtcgc aaaatatgtt
    10741 ggagacgaag aagcttctat tactggtttg aaagagatgg tatttcctgc agcgggggag
    10801 taactagccc cgttgttgga gcaatcactc ccactattaa tggggcaaca gttgaattaa
    10861 gttaggagga taactatgcc agatacattt aaaatctata aaaaaaatgg tactgaattt
    10921 acaaaagtcg cagagggtga aagtccactt tctattactg gaattgcagc aaatactcaa
    10981 gttgctaaag gagattatca agctacgcgt gtggtcggag gtcaagaatc agtaaaagta
    11041 gatattcctg cattcaccac tcttccaatt tcagttactg gtgtgacgtt ggataaaacg
    11101 acagctgaag ttgaacaagg cggaacctta aaattaactc ctactgtaac accagcaaat
    11161 gcaacagaca aaacaggaag ctggagcagt tcgaatacag ctgtagctac ggttactgga
    11221 ggcaacgtta cagtaaaaac agacgctgaa gttggcggaa ctacagaagt ttcatttact
    11281 actacggatg gcggtaaagt agcaaaatgt actattacag ttattgaaaa agcagcgggc
    11341 tagtcttcgg actagctttt ttatttggaa aataatggag gaatctaaat ggcaaaagta
    11401 agaattgaat tgaaagaaga atcaggaaag aaagtctatg aaaacataga cacaacaggg
    11461 aaagattatc gaaaagcttt agaaacaatc aaaaagctaa atgaagacgg agtaatgatt
    11521 tggaatcgtt tagacattta tttggatttt gctgtaggta tcttcaggga tagcaaattg
    11581 acagctgaac aaattcttga tggcttacca tctgagaagg taatgagcac attggacgac
    11641 atcctaggag aagtaatggg gattgaaagt aatccagacc cagacgcaaa aaagtaacac
    11701 ccgaagaagc agaagaaatg tacttaaacc tatgcagaga gttagtgaaa gcgggatggt
    11761 cactatctga catagaagat aattcctttg aaatgatgat gaaaattgcg tgcacttcgc
    11821 ctaagaaaga gaaacaaaaa gaagttgatc ttaaagactt cttgaaatcc atgtagagag
    11881 gaggtaaaca tatggcaaat ggaaaacctt taggcaatat gattatcaaa ctagacttag
    11941 acagttcagc tttttcaaaa ggtctagccg gagctaaaaa cgctgttaac catcaaatga
    12001 aagcaatgaa atcacagatg caagtaatga actcttcagg aaattctctc ggtgctttac
    12061 aagcaaaata caatggttta ggcggagttc tacaagcaaa tgaaaagcaa gtagaattgc
    12121 tcactaaggc ttataaggat agttttgact ctaacggaaa cgctactgct tcgactgcta
    12181 aatacgctaa tcaattgaat caagcaacag ctagatcagc aagttacgaa tctcagatga
    12241 aaacaactgt tggacagatt gctcggatga aagtggaaac ggaagggttt acggggaaac
    12301 taaaggctaa ttctgatgta ttgattaatt ctgggaaaaa aattgaagcc tttggtaaag
    12361 gagtttctgg gataggatct gccctaactg ttggagtaac agctccaata gtggcaggcg
    12421 caactgctgt tactagtgca gctatttcat gggaaagtgc ttttgcaggt gtaaaaaaga
    12481 caaatgatga agttgtagac tcgaatggaa acgtagttta ttcctatagc gacttagaga
    12541 aaggtctgcg taatcttgcc acccaattac ctggtagcca tcaagaaata gcaaatgtag
    12601 ccgaagcagc cgggcaatta ggtataaaga ctgaaaacgt agttagcttc actaaaacga
    12661 tgatagacat gggtgaatcg actaatatgt cggctgaaac tgcggcaact tctttagcac
    12721 gatttgctaa tattacccaa atgtctcaaa aagactttga taaattagga tcagtcattg
    12781 ttgatctagg taacaatttt gctacaaccg aatcagaaat cactgaaatg ggattgcggt
    12841 tagcgggagc tggtaaacag attggaatga gccaaggcga aattctagga tttgctactg
    12901 ctctatcttc tgttggtgtc gaagctgaag cgggtgggtc tgccttttcg aaggtcatga
    12961 tccagatgca actagctgta gaaaaaggat ttggcgcttt tgatcagctg aaacagatgg
    13021 cagaagaaca aggtgttccg tgggttaatt tagtaaatgc tgtgagagac ggtggaaaat
    13081 cgttaaaagc agtatcagag caaatgggat tcacttcatc tgatttaaag aaaatgtata
    13141 aagaagcgga caattcgaaa agttcgcttg aaaattttgc gaatgtggca ggtgttacaa
    13201 gtgaacaatt ccagaagttg ttcaagagtg atccttcaga agcaatcatc aagttcattc
    13261 aagggctgaa agattcagaa aaacatggta cttcagcaat caaagttctg gatgacatgg
    13321 atataaaaga agttcgacta cgcgacagtt tattgagagc tgcaaatgct agtgacatat
    13381 tcaaaggagc aattgaaaaa ggaaacggcg cgtggaaaca aaatacagct ttaacaaatg
    13441 aagctaataa aagatacgaa acaactgaat caaagctaaa gatattgaaa aacgaagtga
    13501 ttgatacagc aatcgatctt ggaggtccat ttgtagacgc gttgcgtgat ggtctaaatg
    13561 tatcgaaacc attgataaaa aacttaggga atatggcgaa agctttctca gctatgagtg
    13621 atgatcaaca aagaaacatt atcaaatggg ttggattagc agcagcagct ggtccagcgc
    13681 tgaagctttt aggaactggt acttcagtaa taggtaaagt tacttcaact actggaagat
    13741 tgacgaaaag ccttgttgat ttagcagcga aagcagcaga aaagaaggct atggatgcct
    13801 ttgcatcgtc ggtaacaaca gctggtgctg cagctgcaaa gacagcagga gctggaggta
    13861 ttggcgggtt agtccctatg atgggaaaaa cagctagtgc tgcaagtact gctgcgggcg
    13921 ccagcggaat aggcgcaatg acaacgtcac taggtttgtt aggtcctgct ctacttggta
    13981 ttgttggcgt tggtggcgca ctggcagttg gctatggagc atggaaatta tttggtgaag
    14041 aagcttggaa ttcctcgcaa agagtaaagc aatggggaac agatgtcggt gaggagatag
    14101 atagtactct tgacggagta cagaagaata ttgaaggtgc aaatggtcaa ttcggtcttc
    14161 tcaaagaggg ttttactgtt gatgatgcaa ataatatggc ggctaatttc gcaaccgctg
    14221 gaacatcttt ggaaacatct ttaacgggta gaataaaggc attagacgat gcactaaaag
    14281 ggctacctca atcagttcag gatgccatga aagaagtagc tgaatcagaa gaagaaacct
    14341 tagggcaatc tctttctaaa ataaaagaca acaatgaaga aataaaaaaa atcagagaaa
    14401 gagccgcaaa tcagaacaga gacttgactg tttcagaact acaacagata caggatctaa
    14461 tgaaaggaac ttctcaagcg tatgtggata cgctcgatgt ttctgctaaa gacagaaaaa
    14521 agatactatc agccatgaat ggtgatgttg cacaagctac agaagaagaa gcgaagacgt
    14581 ggttacaatc tttagcgaaa caaaaagctg caactaccga aaattataga caaatgcttg
    14641 aagaaaaaaa ggcggcttta aaggaaaagg gagtaagcaa agaattcatt gatgcattag
    14701 aagatgaaat gaatgattac atcaaaacta caaatcaagg attcgatagc caaatagctg
    14761 ctataacaaa taaatatcca caattgatta acgaagtgag tttatcaaat ggtcaattat
    14821 ttagcaaaac ggcagctgct atggacaata ctggtcagta tacaaaagtt tttctcgaaa
    14881 gtaatgaaaa gattttagcc caagcgaaga aaacatcgga tgaaatagca gataatgccg
    14941 aaaaaaatgc agaacaactt ggtatggtag ctgatgaggc tacgcatgct ggatcaactt
    15001 ggaatggctt agttttagac gaaaaaacag gaaaagtaaa aactaatgta caagaagtag
    15061 tgaatgaagc aacgaaggat ttcactaaat ggaatgaatt gaaacctgtt gttcatgatg
    15121 cgaaactaac aacgaatgca aaagatgtca tttccatagc ggcagtacaa aatggctatt
    15181 gggatgatat ggaatggaaa gaaaaagaag cattattaga agatgagtgt tctaaaaatg
    15241 tatacaaggc tttagaaaat gctggtaaat ggaatgagtt agatattcct tctaaagaag
    15301 caatcctaac ttcaaatact cctgaagtta tgggtgaaac tctcatgaat ttgggattat
    15361 gggatactta caaacttcaa gtgaaagatt tagatttgaa tgcgtataaa gtatacgatg
    15421 ctatcgatgg atcagaagaa aaattaaata gttgggctca attagataac cccacaaaag
    15481 aattgttatt agataactca gatttttcta caaaactttt taattccgaa gaaatgttta
    15541 aacgttggaa tcagttatca tctaatgcaa agtttctttc ggctaataat actaaattta
    15601 cagaggttct tttatcatct gacagaatgt ggaatcaatg ggtaaacctt cctgataatg
    15661 agaaaagatt acttgcagat aacgaagatt tagctaccac tgttttatct tcagaagaaa
    15721 tatacggaag atggactcaa ttaccagata atcaaaaata tatgttagct gacaatactg
    15781 acttagcaaa caaaatattc gcttcagaag aatcatggaa tgcatggaaa gcaattccag
    15841 acacagttaa aaacttgctt ggtaataata ttgatttact ggctaaagtg aaagacggga
    15901 ctgtaagtgt agaagaatat aacaaagtta tgccacttct taaaactcta tatggagata
    15961 acgttaattt aaaagaggca atcagtgggg ctaaaagctc tatagagaat tacaacaaaa
    16021 atatttttcc tagtgataaa actgcagtag gacacgatga ggcttcacaa gctgctaaag
    16081 atgcatttga agcgttcaac atcttccaaa caaaaatacc agataaaatt actaaaactg
    16141 tatcagctga ctttataggt cctatgccga aaaatgcgaa aggaacaaac ttccatcctg
    16201 gcggagcagc tatggtaaac gatcaaaaag ggcctactta tgaagagctt ataaccttgc
    16261 caagcggaga aacttttata cctaaaggac gtaatgtcgt tttagactta ccaagaggtt
    16321 cgaaagtact gaacgccact aaaactaaac gtctagtgcc taaatatgct gatggtatag
    16381 gaaacataac gacggtttca tctacgctaa acttagatgc tttaatttta gctattaacg
    16441 agctaacgac ggtattgaga actcaactga tggcaggaac gactgatgct aaagatacta
    16501 aactatcaga tgaaacaaaa gttgccgatc cgattattcc ggacagtctt tctgaaaaat
    16561 cagatcagta tctaggaatt ggagctcaat ggttaactaa tctaatgaat ggttggaact
    16621 cagcggttcc tcaatatatg aatagtgaaa tggtttttat tacaaactat ctcaatgcgc
    16681 taaaacttca aaacaatcct aattatacgc aaggagcaac atggaataag aacttactga
    16741 atggatggaa tagtttaact gggaccttta ttgctacaat caattcattt tgtaatcaag
    16801 caatggtcac gcttagaaac tacaacactc ctatgtacaa caatggccga acgtggcagc
    16861 aaaataatct taatggttgg aattcattgt atggatcatt catagcccgt gtaaatcagc
    16921 ttggtaacga ttcgattaac aaccttcgtt ctaaaagtgg tggtttttac aatgcaggga
    16981 cattcttgtt gcaatcttta ataaacggaa tgaattcgtt aggtaattct ctttctacta
    17041 caatgaacaa tgtcgctaat acaatggttg gtggtatagg caaaggagtt aatggcgtca
    17101 tatcgggagt taactatgtg cttaaggaag tcgaatcaag taagaatatc ggaaactggc
    17161 caattccaca atacgctaag ggaactgatg gacatcctgg tggacttgct atgattaatg
    17221 atcaaaaagg cctagttcac gaagaatatg ttcagatgcc agatggacgt agttttattg
    17281 ccaaaggtag agatcttctt gttaatttgc ctaaaggtgc tcaggtctta aatgcttctt
    17341 taacaaagaa attaaaggaa cgtttaaata ttccacacta cgaaaaaggt attggcaatt
    17401 tagatattgt tgatttgtta gatgatgaaa aaagaatgtt agaattctta actagcaaag
    17461 ttgatttttc aggcattaat gaaccatggc ttgatatgac taaatcagga acgtcattaa
    17521 tgtccaaagc tgcaaataca atgcttcaat caaaattaag tgaattcttc actcatggca
    17581 attttgatgg tgcagtaaat gccaatggtg tttatcaata tttagttgat gtggcacaga
    17641 aagtaatggg taagttcccc ggacttacag taacttctgg atatcgagca ggagatgcct
    17701 actatcacgg aaaacggcaa gctatcgact tggcttatcc aggtatttct ggagatccga
    17761 gatatacagc agcagcaaat tacgcttttg aaaaattccc ttcaaaaatt gcgtatgtca
    17821 ttacgaatgg acgtgtacgt gaccgcatgg gattatctgg aacaaggtca agcggacaat
    17881 ggacgaattg gccagatggc gatcacttcg atcatattca tttaaacggt tcgatggggt
    17941 ctggcgatat attcaatgga ggagttggag gaagtggtgt tgcacgctgg cgttcttatg
    18001 tgtctaaagc attgaaaatg aatggattac cagctacagc agcgtatata aatgcctgga
    18061 tgtcccaaat ccaaacggaa tctggtggta atgagaaagc aatcggcggc aatgatgggc
    18121 tcgccgaagg caatgcaaca ggattacttc aaacgaaacc cggaactttt gcagctaacg
    18181 cttttcctgg tcacggaaat attatgaacg gatttgataa catgttagca gcaatcaatt
    18241 atgctaagaa acgttacgga gttgcaggta tgttgcaagt tatcgggaaa ggacatggat
    18301 atgccaatgg cggcttgatt accaaagacg gactatatcg agcaggagaa ggaaataaac
    18361 cagaaatggt tattccattg acaagaaaaa caagagctat cgaacttatg ggtcaagcat
    18421 tagccttcct ttctggaagc aataaaaaag aaacgaaaag tgagattaat tctgtagata
    18481 atagacatat cctacaagcg atagaaaaac agtctcgtat gacaaatgat ctgctatcat
    18541 tattactctc gttttttaaa agtaacaatc gatcagataa agaactagct ttagatattc
    18601 agaaaatttt aatcaggagg acatagtatt tgaggacagt aatattaaga aataaattga
    18661 atgaagaaat tgatttgtcc acagaagatt attttgctac tgaaatgtcg aatctaggtt
    18721 ttgaagttac aaaagagcat attggacaat gggggagttt cagagaaata ggagagagta
    18781 ttgaattgtc tgaatttcaa tcatcagtta tgatttctgt tcacggattt agagaacaac
    18841 aattatatgg ttcattagtt gaatttctgg ctaatggacc ttttgaacta gaattttctt
    18901 ttgattcaga aacaatgatc aggaaatgta gtttgaaatc attatctaaa acagaaattg
    18961 attcccaaag ctcattgcta acagatactt tgaatctata ttttacttca agctggtatt
    19021 ctattaagcg tgaaaaatta atacagaatt caacttctct caatacacgt ggaaaagttt
    19081 actcatttgt tcgaccatat gtctacacca aaaatgtatg ggaaaagaaa ggtgtattta
    19141 agttcagtaa taattctgta tatttgacaa atagtacaga gcgaatgtct ccgttaaaaa
    19201 taaggattat tggagagtgc tcaaatccat attgggaagt tatccaaaac tctaaagtag
    19261 tagcttcaga tggctatttt attaatatga aagacacaca aatattagaa gtgtctagtt
    19321 tgctagaaga ccaaacggca attttaaaag atgtaggtgg agttgaatct tctgtttatc
    19381 aacaacaaga ttttactaaa tcaaacttcg tccaagcgcc tctaggagaa ttcagcgttg
    19441 tttttcatgt tggacatgca aatgtagaga ttgaacttta tgaggagcgt gatctatttt
    19501 gattttagcc attaccctct ttcaccgaga tatgaattta tataacgaac gtttaatttc
    19561 gactgagttc gaatttggaa cggatgaaat tggagaagaa gtaagtaatt ttattacaga
    19621 taaatatgtc catgtaaaat caggagattt tctactagca aaatttattc ctagcgggaa
    19681 gattgcttat tttggagtga ttagctcgca agaagacaaa aaaataaatt gcaaaggatt
    19741 actcagtttg gccgatagcg agataccgac tgtaaaaata tcaggtgcta attatgaaga
    19801 gcatattcga cgactgattg aatactactt attgaacgat ccaacaaaac aactaaaaaa
    19861 tattttggag gtaaaaacag aaagcgttac ttctcattct tatcaagctg ttgacacaaa
    19921 taagagacga ttgaatgcat atataataaa tgggttcaaa aaatataatg ttaaatggta
    19981 tgtcaaagag attaaaggta aaaaaatata tactggtatt cgagcagttg atgattcaat
    20041 ttatattaaa gataattcat ctgagtttaa tgactgggat gtttttgtgc aatctcctgg
    20101 accagggaac gagaacaaat tattgatcgt agataaagca atgaaagata tagaaaatcc
    20161 cttggtattg tctacgtggt atttagatga cgaaaataat cttacgcaag attcaacgaa
    20221 taagaacatt gcattgccta ccgttaattt agtaaatata tatgatcaaa ctcaagaaga
    20281 tagaccttct tatgaagagg tagctaaatc ggagcttaaa ggcaacgcct attcacatga
    20341 aattaaagtt aatgttgtgc taaatgctag aaatatcaat gtagaaaata tcgaaacagg
    20401 aatgttagcg actattgttt acaaagagca gacttacaaa tctgttctaa ctgcctggcg
    20461 aatatccagc agtaaaaaga atatagaatt gacatttggt aatgtccgaa gtcgttttat
    20521 ggattatttt gaagataatg ggggataaaa aatggttagc aatgtggatg gatatcaatt
    20581 tgaaaacgtg aaagtaagcg cagaaaatga tgctagactt tatcacgttt tatataatcg
    20641 gaaaaatcag gttattgatg gttacgatca gtctatgaat ttatcttcaa gcggattaac
    20701 agtaaaagtt gctgcaggag cagcgattat tcaaggtcgt atggtcgttg ttcgacaaga
    20761 agaaagtata acagttccag caaactcaag tggttatata gcattaacag tggatttgac
    20821 acaagaagtt atacctggat ctattcttcc agaatcggaa gaatatgaat ggactaataa
    20881 tcaagtcaag ctagaattta taacaaaagt tataaaaggt aatttaaaca atggtgataa
    20941 ggtctataat ttaccactat gctcagttaa ttctactgga tcaactgttt caatctcaaa
    21001 gatatcggat agttacgagc tgactctctc taaaggagaa attttgtgga gggggactgc
    21061 gttaatgcat gatactcaaa ctgtccaacc ttcaaaaaaa atttggcaga cagtttcagg
    21121 ttttttgtta gtatggctcc catacgaaaa cgggcaagca attgaggata gatatgtaac
    21181 tacgcctttt tataaggagc gtataacttt tactaatgtt ttaggagaaa ttgtttcagg
    21241 atttgatgac tatcacaaaa agtggtttag taaacgaata aactataatt cgaatactaa
    21301 tatatttaca ggtgcagcaa gcaatgcaag tggagataac gcaaatatgg tgcttaggta
    21361 tatagtttct ttttagttag gaggtatgga agagtggcaa atttagaaat taaattatct
    21421 gcaaataaga gacagcctta tctacgtcac cgtgttgttg gtagagttgg tgatgggggg
    21481 ctcacaacaa tcaatgtaca acttcttgag gaagatgaaa ttacaccttt tgtaattaat
    21541 ctaaacggta ctttgaaatt tgtgggcgaa gtttcaaacg gtaactatac cgagggagaa
    21601 ccagaaataa tcgattcgac taatgggtta attagttaca cgttcactaa gtcaaatttc
    21661 agcacgagtc atcaattcaa acaagcatat tttgaatatg tagatcctaa cggcaaaaaa
    21721 gtaacttttc agaacttcat catagacgtg ttagaacgag tagatattaa ttcggagcaa
    21781 gcgaaatact atatttcttc attggaaaaa ttacagagtg aaatgcaaac cactttcaat
    21841 cagttcatta gtgataaaca ggtccaatac gatcaaatct actcgaaata taacgaatta
    21901 gtaagattga taaatgaatc agataagcaa gtaaatgatc gtattgataa ggcaaataat
    21961 cgtattgatc aaaccaatca gcaaatcggc gatctcggca agctgaaaaa gatgtacagt
    22021 aacagcattg a
//
LOCUS       DARBEC010000046        22030 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.046, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000046 DARBEC010000000
VERSION     DARBEC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22030)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22030)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22030
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.046"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(61..1371)
                     /locus_tag="SCL80_001597"
     CDS             complement(61..1371)
                     /locus_tag="SCL80_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1107467.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     INFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1383..2801)
                     /locus_tag="SCL80_001598"
     CDS             complement(1383..2801)
                     /locus_tag="SCL80_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107468.1"
                     /translation="MLSQNAVTANTVKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3041..4609)
                     /locus_tag="SCL80_001599"
     CDS             complement(3041..4609)
                     /locus_tag="SCL80_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEG1107469.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(4613..4894)
                     /locus_tag="SCL80_001600"
     CDS             complement(4613..4894)
                     /locus_tag="SCL80_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1107470.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAAGAFSLYRVRMRKGA"
     gene            complement(4891..5814)
                     /locus_tag="SCL80_001601"
     CDS             complement(4891..5814)
                     /locus_tag="SCL80_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEG1107471.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(5811..6689)
                     /locus_tag="SCL80_001602"
     CDS             complement(5811..6689)
                     /locus_tag="SCL80_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEG1107472.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPTVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIETLLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(6676..8013)
                     /locus_tag="SCL80_001603"
     CDS             complement(6676..8013)
                     /locus_tag="SCL80_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEG1107473.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTSAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8016..9119)
                     /locus_tag="SCL80_001604"
     CDS             complement(8016..9119)
                     /locus_tag="SCL80_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEG1107474.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9193..10701)
                     /locus_tag="SCL80_001605"
     CDS             complement(9193..10701)
                     /locus_tag="SCL80_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1107475.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(10667..11359)
                     /locus_tag="SCL80_001606"
     CDS             complement(10667..11359)
                     /locus_tag="SCL80_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1107476.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11381..12355)
                     /locus_tag="SCL80_001607"
     CDS             complement(11381..12355)
                     /locus_tag="SCL80_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107477.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            12655..13527
                     /locus_tag="SCL80_001608"
     CDS             12655..13527
                     /locus_tag="SCL80_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107478.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14109..16307)
                     /locus_tag="SCL80_001609"
     CDS             complement(14109..16307)
                     /locus_tag="SCL80_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107479.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLFSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16539..17531
                     /locus_tag="SCL80_001610"
     CDS             16539..17531
                     /locus_tag="SCL80_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107480.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNLAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17591..19012
                     /locus_tag="SCL80_001611"
     CDS             17591..19012
                     /locus_tag="SCL80_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEG1107481.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19005..20504
                     /locus_tag="SCL80_001612"
     CDS             19005..20504
                     /locus_tag="SCL80_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEG1107482.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20543..21937
                     /gene="uxaC"
                     /locus_tag="SCL80_001613"
     CDS             20543..21937
                     /gene="uxaC"
                     /locus_tag="SCL80_001613"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1107483.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYLTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
BASE COUNT         6670 a         4494 c         3823 g         7043 t
ORIGIN      
        1 atcattaaaa aaaataaagg tcaagctaaa atattattta agcttgacct tattataatt
       61 tcaacgattg tcaaaagtat tgtttgcagc tgtgttgcca gtttctatag ataaatcaca
      121 gcttttgaaa cataagtttt gtactgcttc gattcgcccg aattcttttg cagttatttg
      181 gcaatgagaa aagcagacat cttctatagg cttttcagga aatgctacta aatcaaaagc
      241 tcgggaagga agtgagtaac ctggagaaag gcatgcctgt acgtttttga tgtgtatgtc
      301 tttgactttc gtcatttgct ttgattcagg tatttcttca gccactgctt gccaactttc
      361 tggcatttga gcaaggtcat tgctattttt tctattgtat tcattatgcc agtccattat
      421 ccaagaaaaa ggaaattgta catcaatcat attcagattt tctaccctga tattttcgat
      481 cactccccct ctttcttttg aagatttcat tcgaatacca cagtctgact gaaaaaaatt
      541 gatatcgtga atatatacgt cactaactcc agcactaact tcgctaccaa tagttacacc
      601 gtatcctgaa cggattttac atctagcaac ctcgattctt gatgattttc gattgactct
      661 tgcaccattg ccatctctgc cagacttaat agcaatacaa tcatcaccac aggccaaatc
      721 acattcatag acacgcacat ttgttgaaga atcaatatca atcccatctg tacttggtcc
      781 atcattgttg cggatgatta ctttttctac taatacatcg ttagaataag tgatttgcaa
      841 gttccaaaaa cctgatttct gaaaaatcaa atcacgaaca acaacatgtt ctgcatgata
      901 cagtaaacaa gcgcgtggac gtttaatagc ataatccgca atccaacgaa gtcccttttg
      961 atcgtattca gctcttgtcc cgcttttttg atcttttccc caatacagct cccaccaatg
     1021 gtctccttga ccgtcgatta ttccttttcc ttctataaga atatctttcg ccgagagaac
     1081 attcaaaatc gctgctggcc atttcatttc tacgccggct actcgtgtat cgatttctgg
     1141 aaatgcttcg atatctgttc tccccttgag gactgcacct tcttcaaaga taagatgact
     1201 cttatctttt aaaaaaaggg ctccgactaa ataaatgcct tttggaataa atacaggtaa
     1261 gtgttctttc gcaccttgat cgattgcttt ttgcagtatt attgtatcat cttgttgagt
     1321 gtcgtttgtt ttaaagttca aagcattgat aatccctttt tttgtccgca atacgtttct
     1381 ccctatcttt cattgatttt tttcaattct tcaccaataa atacagtttt tgaacgattc
     1441 agtgggtaac ctactagcaa aatgattgcg gatacaaata agcagaatgc tggtaacaag
     1501 tttgccatcg tataaatcct ttcttgtact aaaactgatt ggatggctcc acctgttgta
     1561 gacgattggt aaccaatgag tgccagcata aatccgccta ccgctcctgc acatgcttgt
     1621 cctacttttc tcgcaaaaga atttactcca tatactgttc catcttctct taagcctgtc
     1681 ttatattgat gataatcaat cacgtcagta atgaatgccc agaccatcaa attgaataaa
     1741 ccagatccta aagtagcgat aaataaaaga atcagatata cccatgcatt tgttatatga
     1801 aggaaataca tcagcaagta catacatgaa gaagcaagta acgcaaatac gcttgcttcc
     1861 tttttaccaa ataattttgt cagctttgta gcaaatggtg ctaaaactac tactgttccg
     1921 taagtaaata aaagagcaac agacattgct tgtttattat ggaagtaatc attgaataaa
     1981 taagtcatat tcgtcccagc taaaatctga ttgatcacaa tgaaaatgtc aacaatgact
     2041 aaaataatca gtgctcgatt agctaacaaa ccttttacca actgagaagc agatactttt
     2101 tcgtttttga caactcttac tctttcagta gtcaatttac aagttaacat atatgcgaca
     2161 aatgctattg cagcacatcc gattgaaatc caaaagaaat gattccctga aacgacttgt
     2221 tgtccactct tgttatgggt atacattaaa atcggaatga agaatcctgt agttgatccg
     2281 ccaatcgcag aaccaatgct tcggaacgtg gacaacgagg tgcggtcatt aggatttgag
     2341 ctaattgctg ctgccattgc tccatacggg atattgattg ttgagagaaa aatcccataa
     2401 acaatgtatg aaacaaaaat ataagcaatc ttcaccgtca ttggaagatg ttggacgaat
     2461 ggcaaaaaca aaatgacagt cactaagcaa aatggatact tcatttttct gatccaagga
     2521 ataaatcggc cagtttctgt taactctgag acatcacata atcttccaac catcacatca
     2581 gcaaacgcat ccaaaattct cgaaatcaag aataacgtac ctacgattgc ccctgaaacg
     2641 ccaagaacat tcgtataata aatcatcaag aaactattga ctaaacctaa aataaaacag
     2701 ttacctagat caccgaacat gtagcctatc ttatcggaaa tcccaaattc ctttttttct
     2761 ttttttactg tatttgctgt aacagcattt tgtgaaagca tttgaattcc tcctacctct
     2821 tctagtaaac gttttcaaaa ttataactct ttttttcttg tttttatatt cacaatgttg
     2881 gctttttatt gcattatctt gacagataaa aaacaaaaaa taatctaaaa acattgatgc
     2941 ataagattga taactttttt tacataacaa tatatacaaa caaaaaaatt cactaactag
     3001 tgaaaataag ttagtgaacc tcatttatta aagaattaaa ttacagatga tcttttccat
     3061 catacttgaa aaactcaaaa tcagcataac ttcctctttt tttatttaga tcatgcactg
     3121 ctatcccgat aaaatttcca gtaaaccctc ctgagagtat acacatgttt ttttgcggac
     3181 ctactttctt ccattcttca gttgatacgt ctttcaaatg aaactgtgct gtctctttct
     3241 ttacagtgac tttcaaaaaa gtcttctttt ctgtgagtgg aatcgtccaa tcttctattt
     3301 gtgctactcc gttttctgat tctatcatac gtaggcattt tcccttgtcc tcattccaag
     3361 taacataaca ataaatataa ttggattcat ttaagtacag taccagtcca gccatctgat
     3421 taaaattatt aggcatgtat tcaaatgctg tctccgcatc aaaagtaaaa tccgtttgcc
     3481 gaatagctaa caaatgatgt tcgaaggtag attgaagaga ttcaccggaa atcagacgta
     3541 aaaaacctgg tctacatttt gtatcacacc aatcagcatt tggcaaaatg cgtctggcat
     3601 tccagttctt ctttagctct ccttcaaatg tatccataaa ctgatgatct ttttcttgtt
     3661 gtacctcttc atttgtctca atcattattt cttccatcgg agttgtctgg ctatctttaa
     3721 gtcttaacca atcgtcggac gtccaataca cttcttgaat agcggtttct cttcccaaaa
     3781 tagccgcacc gtttaatggt ctggtacaaa gatatgtcat gtaccatctt cctgacgggg
     3841 tttgtactaa gctcccatgt cctgtacatt gcaatgtaga agcaggtttg tcactagctg
     3901 tcagcatagg aaaaccagga tcaagctcgt aaggaccagt aatattcttt gctcggcaaa
     3961 ctgtaactga atgtcctctt cctgtaccgc cttctgccgt catcaaataa taatacccgt
     4021 tacgaaaata caaatgtggt gcctccgttt tcgctaattc tgtacctgca aatattttat
     4081 agatgggacc atcaagcttc ttcttctctg catcatattc ttgtaatacg ataccgctgg
     4141 atttattcgg tgtgtccaga cgataatccc atagttcatt gacaagccat ttttttcccg
     4201 tttcaagatc atggaacaaa gaaggatcaa agccgttact atttaaataa attggttcgg
     4261 accaaggacc tgtgatctca gatgcagtga tcaaataatt gtggacatct ttaaacggac
     4321 ggcctgcact tttgacatct gtgtaaacaa gataaaacaa tccatcagca taactgatct
     4381 gtggcgccca tatacttcca tttgtaggat ttccttgcaa agatacttga gtggtcaaaa
     4441 tatccgttgt atgtgaccag ttgactaaat ttttcgactc atatactcgg attcctggaa
     4501 gccattcaaa agaggagaca ataatgtaat accgctcttt cactcttaat atattaggat
     4561 ctggattgaa tcctcttaaa ataggatttt tcactttgat agtctgcatg attcaggctc
     4621 cttttctcat tcttactcga tataaactga atgccccagc tgctaaaaaa gcaagtgaaa
     4681 taaacaacac aacggcaaac agaccagtca tgtcctgtaa taatccaaat gtattttcta
     4741 aagacaaagt tgaatgatct acagcaagcg actgataact ttttttaaac tctgacgcag
     4801 acatattact taaagtaact gctaacccgc caaagaaaat caaagataga ctgccaccga
     4861 ccattcctag caatagttcc cattttcttt tcatttctga accccctgct tttctaaatg
     4921 tatccattca ggtcttttga tcgatgtttc ataattgtta ttacgttcag agaatttgac
     4981 agtctgttca tttttcttgt catcccaatt atcccaacca gttgggtgaa catgcccacc
     5041 tatcttacac gcttcaaata agacatttgc atacggacgc cacggtcttc ctaaatagac
     5101 gtttctagta tcaggttctg cagtcaaggt acagctttca aaacgatacc ctgttttttg
     5161 atttttcggc gtagatgctg ctgtgatgta tccaggattt ttactggcta agctatggat
     5221 atgacactga tgaaaaacgg catctgcccc accaaatatg aaatccaccg ttccttcaat
     5281 ccagcaatat tcataggtct gacgacaata ctcaggattg gcagaagccg gtgtacaaaa
     5341 aggagtacca tctttttgca aatcaggcaa cggacccgtg cataaggtat cctgataacc
     5401 agatagtcga cagtttttta aagatacgcg atctcctgaa ttgaataaag caacagcctg
     5461 tcctaccttt tctcctggac ctgctgtatt ttcgatgtga agattttcta atacgacatt
     5521 cgatccttct aagaaaaaag tagcacttcg gaaagtccct aactggctac cgtcaagata
     5581 ctcctgtttt gcaaaacgat taccggagat aactactgtt cctgctccca caagtttaaa
     5641 attggataat ctgctttcta tatattcatc atatcttcct tcttttacta tcaagacctt
     5701 tggatggcta tccggcaggc ctgccaaata atcgattcct tcttggatag tttcaaaatc
     5761 cgtcggggta tctatagcat tctttccaat tatcagtttg tctttcatcc tcataaatgc
     5821 tcccttatga attgtttcca aatatttctc atttcttgtg tttccatatg accgcctgtt
     5881 aaagaaaaag aagaaaagtt ttctggacta cccattgatt gatacttttt gtccagccct
     5941 tttctcagct tcagtacgcc ttcgtccaag cacatcgtat cgttttttcc aactaagcta
     6001 agacgagggc gcggagcgat tttttcttgg attgctaacg ttgtataagc tgtcaataga
     6061 tttggcacat agtaataaaa cccatgatga tctaggcggc gatgtagcaa aagtgtttcg
     6121 atgtccacct gtgccgctag atcaatacaa actttgacac gagaatccaa tgctgtcacc
     6181 caccagctta ataagcctcc catagaaaga ccaatcgttg cgagccgttg agtatctgtg
     6241 tcttcccttg tctccaaata atcgatcaag ctgatcacat cataaatacg cattccccat
     6301 aacgtttttc ctgttaataa aaattgtttg aacaactcgc tttcagaaat acctccgcgt
     6361 tcttcaaatc cccaagcatc aatcgaccaa acagcatatc ccattcctgt cagtacttct
     6421 gcaaaagatg gagaagctaa gtaaggagca ccatggatca attcactttt cccgactgaa
     6481 aaatctccgc cgtgagaatg caggtaaagg accgtcggaa acggtccttc tttttctgga
     6541 taagcataga gggctggtac tgtttccaat tcatttaagt acaattgata gtattcaagc
     6601 ataaaaccgt tttgtttttc tcggtataat cgttttccac taggggtatg tctgctagat
     6661 ggagcaccta aaaaatcatt caactgcata agcaaagttt ttacgaacaa ccagttcatc
     6721 catattttct gattgatttg attggaattt ctcaatactg atttctttag tattttctaa
     6781 tagaaatgct ggtccctcgt gattttcaat cgacatctgg ttaaaggaaa ccttacccgc
     6841 aaagccaata taaaatcctt gtctgctcat ttcctctatt cctgtcatca tagcaggaac
     6901 accgggaact gcatcttggg ccatcgagac atcgatattg tcaaatgtga tttcttgtat
     6961 gaattgttcc gctaatccat aaataaaacc tgcagaagca tggacattcc ttgccgtgat
     7021 attcgaaaaa tggatccttc tgaatgctga tgtccgttca tctatcggat aagccttttt
     7081 ttcccataca tacggttctt tccctcgcgg accacagaaa taataaagat tcagtataaa
     7141 tggacacatc acattatcca tgactatatt ggatactcga atatcttcaa cgatcccgcc
     7201 tcgacctcgt ctagatttca atcgtattcc tcgatcagtt tcttggaaga tacaatttga
     7261 aatggtaata ttacgaatac taccactcat ttcacttcct aatacgacac cgccatgtcc
     7321 atggaccatc gtgcaatttg tgatcgtgat attttcgcaa gcgatccttt cgtacgtatc
     7381 ttcagtccct gccttgattg caatacagtc atctcccacg tcaatatggc aattactaat
     7441 acgcacattt ttgcatgatt ctggatcaat tccgtctgta tttggtgaat ccgctgggtt
     7501 caagatcgtc aagttatcaa acgttgcatt actgcaaaga atcgggttga tcgtccaact
     7561 tggtgactgg atcaatttaa tatctttcac agtaattcga tgacagttat ggaaactcat
     7621 caatttagga cgaggataag cgagattatc tggttcattt ctaaaagtat gccaccattt
     7681 ttttccattt ccatctaatg taccgaaacc agtgacagag atgttctcta cattttgagc
     7741 atagatacag gaagcataaa cttttcgatg gactccttcc caacgtgaat gaacaacagg
     7801 ataatctttt ggatcgtcag agaatttcag aaccgctcct gcgcttaaat gaagttcgac
     7861 attgcttttt aaaaataaag ctcctgttag aaattcacct gctggaacaa ctacggtccc
     7921 gcctccgtca cttgctgcag catcaatggc ttgttgtatt gcttcagtat tcaattcatc
     7981 aattgatgcg ccaaatgtca aaatatcata catatttata atgcctccac ttcaatcaat
     8041 gcttgcaaga atgcaccaac gcctttttga tcattacaaa taattggttc actgatgtag
     8101 taagcatatg aaccatctcg ctgatctgct ccgcctaacc ctgctacttg acagttttta
     8161 ttcaagttca gccagccttc ttttgtttcc agaacaaatt catcaagtaa tccttgatgt
     8221 gatttcttca cttgtggaag ccattcttct ttgttcagca gtcccaaacg tattccttta
     8281 gccattgcgt aaacaaacat actactgccg gaagcttcca gataatttcc tttccgtttt
     8341 gtttctgttg ttacttgata ccatgtcccg ctgttttctt cttgatatgg aagtaatgca
     8401 gccagtgtct tttctagaat ttctttcagc aaagaacaat ctgtttcttc atgaagtaat
     8461 tcataggtat ctgctgcagc catcaaatac cagccaatcg atcggctcca aaaatgatgg
     8521 gatagacctg tttttggatc agcccatggt tggaccttct tttcatccca tgcatgaatc
     8581 agcaaatttg tttttggatc gaatagatgt ttataactga tttcaaactg tctcaaaaca
     8641 tcttggtaat ctttttcttc actaaaattc tggataaatt cagcataaaa aggagaaccc
     8701 atatataagc catccaacca aatttgataa ggataaattt ctttatgcca aaaagctcct
     8761 tcggatgtgc gtggatggtt tgccaactgc tgggaaagga gttctgctgc ttttttatat
     8821 ttttcttcgc ctgtttcttt gtacaataaa aagaaaagct ttcctgtatt tacatggtcg
     8881 atattgtatt cttcaacgct gtagccacgg atcgttccat cttcttgcac gaaatagtcg
     8941 atattgtctt ttatgaaatc aaaataaatt ttttcttggg ttcgttccca aagtaattgg
     9001 aaccctttaa gtacgacgcc atagtcataa gaccattttc ctttatacca cttttcttca
     9061 tataagcgag gtgttctttc catgatactt tttgctaatt tctttgacca gttttccatt
     9121 atttgctctc ctttaaaaat taaaatagga tttgggacaa ataccaagaa gtatttgtcc
     9181 caatcgtcta tattactgtg ccgctgcttt gtatgcttcc tcgtactctt gtgtgattcg
     9241 atcgcctcct tgtgctttcc agtcttctac agcttttttg aatccatctt cgtcaagctg
     9301 tcccatgatg tattgcaccg tagcatcaat gacgattttt tcaatttctg atcctacttc
     9361 attgttcgtc ggtgaatcca atggcacaga aggatccatt acagcaaatt cttcattttc
     9421 tttgatcaat tgattagaga attctttttc agggtctgta tctttgaaag agaacgtcac
     9481 ttcactagga cgagagcttg ataacggttg cacctcttgt tgccaaagat ccatatcgat
     9541 aaattcgata gaaccatcat cattcaattc ataatgcgtt ccttcgatac cgccagtcat
     9601 caatttatac acatcttctt ctaaaagatc atcaacaaat tgaagtaatt tcttcaattg
     9661 tgcttcgtct tttacttgag atttgggaaa tgctagcaag ccgccaatac catttccttc
     9721 tgaccaaaca tgataaccgc cgccttcacc atctgagatc ttgtttacag gcactaattc
     9781 catatcatct tgaatacctt gacccatcgt cttcagattc ttgatatcca ccattccagt
     9841 gtagattcca gttcttcctt gtgcaaattg ttcttgctga tcagtctttt gggtaactgc
     9901 aaagtcttgt gccaaatagc cattctcaaa tagatctctt gaaaaattca aagctttcat
     9961 atactcttct gtatcaaatt ctggagtgaa tttgccatta tcgtctacag cccagccatt
    10021 tggtgctcca aaataagaag tgaaaagttt aaagctggta taacgaatat cactaggcgc
    10081 acgatcgcca aaaccaactg tgtcgtccac accatttcca tcaggatcgt tttctgtaaa
    10141 ttgtttagct acttcataaa gctcatccat ggtagttgga acatctagtc ctagattatc
    10201 caaccagtct tttcgaatga ctaagccagc acgagcgtag tctttttgta tcggtacgcc
    10261 gtacaaggca ccgtcgatac tcgcagcttc gattctatct tcagaaattt gacttaaatt
    10321 tttatagtct tttaaatatt ctgatacatc ccagaatacg cctgatttta acgcattgcg
    10381 gacagaagag tttttcaaca tcgcaatttg aagtgtaacg atatctccta gttcatcaga
    10441 agccaatgca gttgttaatc tttcttcttt tgaagcatca ggaatccagt tgaatgtcag
    10501 ttccgtatcg gttttttctt cgatcaagtt cacaatcgtg tctgtaggcg gtgaagcatg
    10561 atgcaaaata tttaaccaag tgattttagc ttttccatct tctgtctctg aagaactgtt
    10621 acttgaactg ctgccgcatg cggcaagtgc tagaattcct gcaaaactaa gacctaattt
    10681 tacgagcgtt gcttttttca taaattttct ccttcactgt tttaataata tttttgtttt
    10741 cttctgtttt ttctgtatat gagttcttcc attccaaatg gcaaaatata gaatttgctg
    10801 tccacgtgac tgcggaaaag aagagactgc caccgataaa ggttaaaaca cctggcattg
    10861 tttggatgat catcagataa atccctacca tacttaatat caacactaat gagtaatgaa
    10921 gatgtgagaa tccgaataaa aatgccattt tcattttgaa aaaatatcct ttccaatcat
    10981 aatgagccat gattgggaaa atatagagtg ctgagagcac atatatgaac gcagcaatat
    11041 acaaagcgat cctaagatac caattgtgta tgaaagaaag atccacaagt aaaacaatcc
    11101 cgatggcgcc atatatccag ctcatcagca aggtttcacg atagttttct ttaaaatatg
    11161 tgaagtacgt tttcgtcaaa ggaaacgttt cttttgagcg cagccatttt ctaatcactg
    11221 caaccatcgc atacgatgca ggaccgaaag taaaaactcc caaacctgcg atgactaaac
    11281 cgccccacag aaaattcaaa taaaccaact gtaaaatacg atttagcctt ccgtttattt
    11341 tatctaatga ctgcagcata tctttccctc tctttctatc ttagtaaacg ccgtcttctc
    11401 ctagcttttt ggcaatctta ttagctaaaa ttaccataaa caatcctacg acccctttaa
    11461 acaatcccat tgctgtactg aaacttaact gtccattctt caatccagct gtataaatat
    11521 aggtgtcgaa aatttctgca acgcttcgat tcaatgaatt caataacaag tacatatgct
    11581 cgaaccctaa gtctaatgtc tgaccgattt ttagaatcag caaggtaatg attactggac
    11641 gaattgccgg caatgtaaca tgccatgttt tgcgtaagcg tcctgctcca tccatttctg
    11701 cagcttcata caactgtgta tcgactgcag ttatcgctga aaggtagatg atcgtagacc
    11761 agcccaactc tttccagatc acttgtccga tatatactgt gcgtgtccat tcaggggatg
    11821 ttagaaaact gattttttca aatcccattg ttgcaaggaa ttcattcaat acccctccgt
    11881 caacattcaa aaatacataa gtaatcgaaa caatgattac ccaagacatg aagtgaggga
    11941 tgtaaatgat cgtttgaatc ccgtttttca atcgtttgtt cgttacttca tttaatagta
    12001 aagacaaaat gattggcatc ggaaaaaaca cgaagatatt taatccgaac aagatcaacg
    12061 tatttcttaa taatgtaaag aatgtcggtt ctgtaaacaa acgaatgaaa tgtttgaaac
    12121 cgacccaagg acttcccatt attcctaaaa atggttgata atcttgaaaa gcaataacga
    12181 gtccccccat aggaagatac ctaaaaatca agaaataaag gactcccggc agaatcatca
    12241 catataaaaa tttattcgtt ttgattcctg caattatttt ttttcgtctt ctgagctttt
    12301 cttctttttt cgatagcgtc agttgaaatg gttcttgttt ctttacactt tccataagcc
    12361 cctccttagt tttgtgaatg tttgctgctt atgacttcat gatagataac tatctgaatt
    12421 acgtatataa tcatttttat cgtaagtaaa aacgcttaca tagcaatgat ttttgtgcta
    12481 tacaaaacga taaagtgtat tttttcacaa ataaactacg catagcaaac cttctatttt
    12541 tttctatctg acacttagca taggaaatga ttatatacgt aatctttcac cgatgataaa
    12601 ttgaacatat agaaacagaa acatttaatt tattttgagg aggagcctat ctatatgaaa
    12661 aagaaagtct ctataggaga aagaagtttt acacttttca acaatacgtt tctaattttg
    12721 ctagctttga tctgtatcgt tcctttttta aatatcatcg ctacatcttt tgcttctaca
    12781 caagaagtcg tcgcgaaaaa gtttatcctt tttcctacta ccttttcttt agacgcgtat
    12841 cgctacattc tttctacgcc gaccattttt agagcacttg ctgtttcgat tggtgttaca
    12901 ggtgtcggaa cgatcgtcag tatgtgtgca acttcactga tggcctacgg tctttccaga
    12961 aagtatttgt ttggtcgagg tttcgtcaac tttcttgttg tattttctat gttgttcagc
    13021 ggggggatga ttccaacgtt cttagttgtt cgttctctag ggctggtcaa ttcttactgg
    13081 gccatgatcc tgcctgtggc tgttaatgca atgaatatga tcattatgag aaactttttc
    13141 caagccttgc cggatagttt ggaagaatca gcaaaaatgg atggttgcac agattttggt
    13201 gtatttttca aaattatgct gccattagct ttaccgtcta ttgctactat ctcgcttttt
    13261 tatgctgtta cttactggaa cacttatatg actgctattt tgtatatcaa cgattcatct
    13321 aaatggccga tacagattct tttacgccaa atcgtcattg tatcaagtgg gatgcaagca
    13381 gaaagtagtg ccgttgatgt gattccacca gcacaaacag ttaaaatggc tgttatcgtt
    13441 attgcaacag tgcctatgct gattgtttat ccttttgttc agaaatattt tgtcaaagga
    13501 gcactcgtag gttctgtcaa agggtgatta tgtactgaac gcaaaatggt agttgtgtca
    13561 gaagtggtca gcttcaagaa ataagaaggc atttacgaaa attgcttctt aaatttttgt
    13621 gaaatttcgg cttatttttc gaagaatcta ctcctggaac accgtgtatc agaggttgtg
    13681 aaagagacgt tcagccttga gcgatgataa aaactctcac aaacattgcg acgagtaacc
    13741 cacaggagca ataagaagcg attttgaaaa atagcttccc atatttcaca aaattatgcc
    13801 ttattgtcga tagcctgtct cttgaacgcc atttattcgt aaaaaagaac tgtgacaaga
    13861 cttttgtcac agttcttttt gcgtgcttag ccttgaaatt taactatcca taaatacaaa
    13921 tactagggtt attctaactt cttttattgc ttgaatcagt ccacaagaaa aacagcctct
    13981 tgattaaacg gcgtttatct tgttgattct gcggcaataa aatcagccag cagcaaaaca
    14041 tgaagtgctg ttttttgctg tcctctttta ttgcttgaat cagatcacaa gaaaaacagc
    14101 ctcttgattc acaaatattt ttttcgatat tctcccggcg taatcgattc cttcttttta
    14161 aagaaacgaa taaaattttg cggatttcga tattgcaatc tttctgcaat ctcttttacg
    14221 gagaggttag tctctttcaa ccattttttt gctacctcta aacgatgatt ttgaacaaaa
    14281 tcaccaaatt tttctcctgt ttccttttta aatacattac ttaaatagtt agggttataa
    14341 tgcaacctat cagcgatcac atctaaagac agatcacgat catattctgt gtagacgata
    14401 tgcataatat tctccgacaa atttttaaat tcatgatcag tgattgcttg agagcattct
    14461 gtgattggca gaactaattg ttctaaaaca agtttttcta gttctttggg atgataattg
    14521 ttcagtgctt ttacataaat ttgtttcatg ttatcaaagt tttttgaatc tactcccaat
    14581 aactgtccta attgaacgag ttcattgatc aagcggaccg aaactacttc ccgactcaaa
    14641 ggatttttat tttgtgcaaa caatgcatca atcaattcat gtactaaatt cttcacttcc
    14701 tgatttccag aacgaatggc ttcaaacaat cgattttgtt cttctatcgg atatctaata
    14761 agtgtttttt catgagcagc aggaacgatt tcctgataaa aaataattga acttggacca
    14821 gtatttacac gataatacag ggcttctttt gcccgatcaa ccgattgctt gctttcattc
    14881 agcgtttcga accggttact gatgcctata ctgatagtta aattcaagta ttcttttaca
    14941 cgtttttgaa tgagtcggca atactccatg acttttttgc ttgcttctgc atcatcttta
    15001 tccattcgat aaatagtggc ttgcatttct tcattcaaaa cgatcggcag cattcgattt
    15061 tcctttggca caatttccgt gatcatgttg ttgatcgcta aaagtagcaa atcacgctcc
    15121 ccaccgtgac tatcttctaa cagatcgatt tgaaccaaag ctgtataata gcagtcattt
    15181 tgagaactat aaccaaattg ctgcaaccgc tgatctagtt ctcgcctttc tactcgattt
    15241 ctaaataaac taagtacaaa tagcgtttcc agctgtggct tttgggtaac taagtgtgca
    15301 cttaacgctt ctttttctcc tatgatccga ctgactgatt gagcgaccaa atctagttct
    15361 ttcccactga atccttttga tttaagattc agacgttcct gtatttgaga aatcggacgg
    15421 gcaaatctgt ccgaaaataa gtaggaaaaa agaccaatga gtcctaccag tgttaaagac
    15481 actgcaacta gcccgattct cagattgcgg atcattgcgc caattgcact agagtcaact
    15541 tctaatctgt aaatccaatt attataatct gatttcagta ctatatacgt tttatctttt
    15601 tctcttatca ctttcagctg attgtaatct agatccacgt ccgttaatac agttggttgc
    15661 tctttatttt ttactgtctg agtagtaaaa aggttgccaa cttcgttttc aatagaaagt
    15721 atgtcttctc cactttcacc aacaacttgt tctattgtat gtttctctat cgtagcaatt
    15781 cccaatgcat atttttcttt ttcaaacatt ggcaaagaaa tcaccatttc gattcctttg
    15841 tttttctttt gccaaaataa attatttttg ttatttatgt acttctgttg atacttttca
    15901 acttcctctt cggtcagttg actcaacgat ccattgatta ctccccattt accagccaaa
    15961 ctgactaaat cataattatt atcatccata acaatcgttt ctataaaatt tagttctttc
    16021 ctgatttcag aataagtgga gtaatctgta tatgtcagcg gatgcttgaa tatgtccgag
    16081 aaatttttgg tcgaaccata cgtagaaaac gaatattcaa tcgttctaat tttccattca
    16141 acgttagttt taacttgcct gagcaaattc tttttgtctg ttaaataact cgattcgatc
    16201 gattcatagg tattctggta aatgaataaa ctaaatccta tgacaagtac ggctcctaat
    16261 aaaaacatcg gtaaaaatat tttataaaat actgtacttt tcttcatttt cttcctctcc
    16321 tccttttaaa ataaaaggct ttcatttttc tgtaaaattg atactcatct aattaaatga
    16381 gcattccccg ctttcaagaa tagcataatt aaaaataaaa atccaagtaa tttttaacgt
    16441 gtgcaatctt ttcatatcaa agatctattg acttatcgta gttatcgtaa tatgatgaat
    16501 gcgttaacaa aaagtgaaaa agtaaggaaa atgatggaat ggtaacgatc aaagatatcg
    16561 ctaaagcagc tggcgtttct catacgacag tttctcgtgc tttgaataac agcagtttaa
    16621 taaaaaatga aacaaaacaa aaaatacgac aactagctaa ggaaatgaat tattcgccaa
    16681 actatagtgc aaaaggttta gtgaatcaaa aaaaatatct aatcggacta tttttctcaa
    16741 gtttgcataa aggaacttca tcaaatttcc tagcagattc cattgcaggc atccattcag
    16801 tattagacac aaattacagt ttatctgttg aaagtatcga tgttgttgat ttaacagaaa
    16861 tcaacctgca acgctacgat ggactgatcg ttatgagtca aagtgatgat gatcagcctt
    16921 tcatcgacta tctcaaagaa aaagaatttc ctttcgttgt tgtcaatcgt cacatcaatg
    16981 atccagcggt tatcaacgtc actgccgatg atgcggccgg tgttatgcag gcagtagatt
    17041 acggtattgg atgcggacat acgaagatcg cttatattgg cggaaagaaa aattttcgtt
    17101 cgacaaaaga acgggaaaaa ggggtattga aaagtctaaa aaaagcgagc atccctgtca
    17161 atctagccta ttttttaaca ggagattaca gcttagaaag cggcctccaa aacatggaat
    17221 acctattatc actttctgac ctgcctactc tggttttttg cggcaacgac gacatagcga
    17281 ttggtgcatt gagagctgta catttagctg gcctccgtgt tccccagaac atttctctga
    17341 tcgggttcga tgattctcca gtcgtctcct atctcaatcc gccgcttacc accgtacgta
    17401 agcctttgaa agaaatgtgt caagaaggaa caaagctttt actagatttg atcgaaggca
    17461 ggacgatcga acaatctaaa attgagctct ccaccttgtt gatccttcgt gagtcgatca
    17521 attacttgta aactacaagt aattttatat aaaaatgtta acgtgtgcat aacacagaaa
    17581 ggaagtttat atgaaaaaac tgacaaaaga attagtgaaa aaaaacgaag cacctgtaaa
    17641 agttcttcag tttggtgaag gcaattttat gcgtggattt attgattggc agattcagca
    17701 gctgaataac caacaattgt ttaaaggtaa tgttgcaatc attcaacctt tagaacaagg
    17761 tcttggaaat atgatgaacc agcaagataa tttatatacg gtcattttac aaggactcag
    17821 caatggcgaa atcgttgata cttccgagat tatcaccgta gtagaacaaa tgatcaatcc
    17881 ttatgatgaa tgggatgctt atctatctct agcagaaaat gacgacttgg cctatatcgt
    17941 ctccaataca acagaggcag gaatccaatt cgtggagact gataatttaa cagatactcc
    18001 cccacagagc ttcccgggca aactgacagc ctttttatac cgacgtttca aattaaacaa
    18061 agctggcttt accatcattc cttgcgaact gattgacaga aacggcgaac gtctaaaaga
    18121 aatcatttta cgctacgcag aaaaatggga tttagaacct gcttttgttg agtggttaga
    18181 aaaagaaaat gttttctgtt gtagtttagt tgatcgcatc gtcccaggtt atcctcgcga
    18241 agatgcaggt gttttcaatc aacggcataa ctatgaagat caattgatgg tcaaagcaga
    18301 accattcatg ctttgggtga tcgaagggcc taaagaattg gagaatcaac taccattcaa
    18361 acaggctggc ttgaatgtca tcatcacaga tgatatgact ccttatcgtg aacgtaaggt
    18421 gcatttgctt aatggaccgc atacagcgat ggtccctctt gctttgttgg cggaattgga
    18481 aactgtagaa gaagtaatga aagatccttt attctccgca tatgttgatc agttattcaa
    18541 tctagaattg attcctatgc tttctttacc taaagatgaa ttagccattt atgctgacca
    18601 aatcaaagaa cgtttcttga atccttttgc ccatcacaaa ttagaagcga tttcattaaa
    18661 tagtgtttca aagttcagca cccgtctgct tccagtgttc aagaagtata tcgaagaaca
    18721 aaatcaggta ccaccgttaa tcactgtttc actagctgcg ctacttctta tgtatcgagg
    18781 cgatcaagta aagccacatg atgatgaaaa gacgataagc gaatttactg atgcttggtc
    18841 tgataatgga acagctattc ctcgattatt gcaaaacgct gcgctttggg gagaagattt
    18901 gtctcaaata ccgaatgtga cagatacagt tcaagaaata gctgcaaaga tcgatcaaaa
    18961 aggtgtccgg tcagtattac aagaaatgaa aggaacgatg gtccatgctt gaaaccctgc
    19021 aaccaacaat gaaattaaat cgacgagaca gtgtggtcgt cgcattaacc cctattccaa
    19081 aacaaacaca actgacgatt gatcagcaaa cgatcaccac tcttgaagat atcccgcaag
    19141 gtcataagat tgcattgggt gatttgaaag ctggagacaa cgtgatcaaa tatggctatc
    19201 caatcggtca tgtgacgaca gacgtaactg ccggtcaatg gctgcacact cataatgtaa
    19261 aaaccaatct ttctggtgaa ttggattatc gatatgaaaa agatgtccat ccaagtcatt
    19321 acctttttga gaaccgaaca tttcaaggtt acttgcgaaa aaatggaaaa gtagggattc
    19381 gtaatgattt gtttttagta cctaccgttg ggtgtgtgaa tggcattgct gaactgatcg
    19441 tcaaacagtt caaggaaaaa catccagatc tgggacagtt tgaccatatt acgattttaa
    19501 agcatcctta tggctgttcg cagcttggaa aagatcatca aaatactcga gaaattttag
    19561 cggacgcagt aaatcatccc aatgccggag gagttttagt atttggatta ggttgtgaga
    19621 acaatacagt tcctgaattc aaaaaaattt taggtgaata tgatgaagaa cgagtgaaat
    19681 ttctagtggc tcaagatgtt tacgatgaga tcgaacaagg tgtggctcta ttagaagagc
    19741 ttttaacagc ggcggaaaat gaccagcgca tatcagtccc gctctctaag ttgaatgtcg
    19801 gtttgaaatg cggcggttct gatggtctat cgggaataac tgctaatcct ttacttggtg
    19861 cattttctga ctacttgatt gctcaaggag gcagcacgat cttaacagaa gtgccagaaa
    19921 tgtttggcgc agaacaagta ttgatggcac gagcggaaaa tgaagaagtt tttgactcaa
    19981 tcgttgactt gatcaatgac ttcaaacagt atttcttgtc ttacggggaa ccagtttacg
    20041 aaaacccatc tcctggtaat aaagcaggtg ggatcactac attagaagat aagtctttag
    20101 gctgcacgca aaaatcagga agctctcctg tcgttgatgt gctacaatat ggcgaaaaaa
    20161 tcagaaaacc agggcttagt ttattgcaag ctcccgggaa tgatcttgta gctgcctctg
    20221 ctcttgcttc atccgattgc cagttggtct tgtttaccac aggaagaggg actccgtttg
    20281 gctcatatgt cccaacggtc aaggtatcta cgaatacaac tctttttgaa cgcaaaggtc
    20341 attggatgga ttttaatgca ggcgtcttat tagaacaacc aatggaagta atcttagaat
    20401 cgtttgttca aaaaatcatc gctgtagcta gtggagaaga aacgaaaaat gaacaaaatg
    20461 acgttcgaga aattgccatt tttaaaaacg gagtaacact ttgattttca taaaacagta
    20521 taaaaagaaa gaaggaagat ttatgttttt atctgatgat tttttattaa aagacgattg
    20581 ggcgaaaaag ctttaccatt cctacgcaaa aaacatgccc attattgatt atcactgtca
    20641 cttagatcca aaagaaattt acgaaaacaa caatttcagc aatctgacag aagcttggtt
    20701 aggaggcgat cattacaaat ggcgcttgat gcgagcttgc ggcgtgcctg aagaaaaaat
    20761 cacaggaaat gcttctgatt ttgaaaaatt tttagcttgg tgtcaaaccg taccaaaaat
    20821 tatcggaaat ccgctttaca gttggtcaca tcttgaattg aagcgtttct tcggtattga
    20881 cttgttgata aatgaagaaa atgcagagaa aatttggaca caagcaaatg aaattttggc
    20941 agctcctgat tttcgccgtc gggaaatcgc aaaaaaatcg aatgtagaag tgatttgtac
    21001 aacagatgat cccgcagacg acttgcacta tcatcagttg cttgctcatg aagaaccaga
    21061 tttgaaggtt ctaccttcat tccgtccaga taaagcactc aatattgaaa aagaaggatt
    21121 taatgcatgg gtcacactac tggaagaagc agctgaccaa acgatcacca cctatcaaga
    21181 actgatagaa gtcttagggc agcggataga atacttccat caacatggat gtcgtgtttc
    21241 cgaccacgct ttagacatct tacgctataa agaagcggat gaaacagtat tagaagagat
    21301 ttttcaaaaa gcacgagcta acaaactatt atcttcagat gaaatcgatg cttatcttac
    21361 agaaacactg atacatttga cccacttcta tcattctcat aattggacga tgcagctgca
    21421 catccatgct tatcgtaact gtaatacaca aatgttggaa aaattagggc ccgataccgg
    21481 ctatgatgga atgaatgatc tttctttgac tattcctctg caaaaattat tgaaccgtgc
    21541 agaagaaacg gatcagttgc caaagacgat cctttattca ttgaatccga atgactatcc
    21601 tgcgatttta gctttgatgg gctgtttcca aaaagaacag aatggaaaac tgcagttagg
    21661 ttctggatgg tggtacaacg atactcgagc tggtatgcgc gatcaattga cacaatttgc
    21721 tgatggtagt gctttgggga attttgtcgg catgctgaca gattcaagaa gtttcctgtc
    21781 ctacactaga catgaatatt tccgtcgtgt tctttgtgaa tttttaggcg aaatggtcat
    21841 gcgaggagaa gcacctgaag atgaacaact tttaggaagt ctagttcaag cgattagtta
    21901 tacaaatgct aaaaattact ttggcttttt tgcctaattg attaatagta acagtaacga
    21961 atcaaaaaca ggatagtgaa ataacggaat ccgtttatcc acgttttaga gaagaagacg
    22021 gaccaaattt
//
LOCUS       DARBEC010000047        21988 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.047, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000047 DARBEC010000000
VERSION     DARBEC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21988)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21988)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21988
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.047"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCL80_001614"
     CDS             <1..395
                     /locus_tag="SCL80_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107484.1"
                     /translation="ISLKDESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHAEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLNQKTIKKLNIGG"
     gene            398..1147
                     /locus_tag="SCL80_001615"
     CDS             398..1147
                     /locus_tag="SCL80_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107485.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKPVRHKYGEYKNVLLSDEQME
                     KLKTEFPNDYQERIERLSEYCESSGKTYKNYLATIRSWARKEKSEPKNASSGYKRTGR
                     REKLPEWAIDQEAYLKKKALERANRQSKAPF"
     gene            1159..1428
                     /locus_tag="SCL80_001616"
     CDS             1159..1428
                     /locus_tag="SCL80_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107486.1"
                     /translation="MKIDYLELINEIANYKKGEELDVLRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            1433..1597
                     /locus_tag="SCL80_001617"
     CDS             1433..1597
                     /locus_tag="SCL80_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107487.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            1590..1892
                     /locus_tag="SCL80_001618"
     CDS             1590..1892
                     /locus_tag="SCL80_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107488.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            1889..2050
                     /locus_tag="SCL80_001619"
     CDS             1889..2050
                     /locus_tag="SCL80_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1107489.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            2047..2352
                     /locus_tag="SCL80_001620"
     CDS             2047..2352
                     /locus_tag="SCL80_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107490.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            2352..2708
                     /locus_tag="SCL80_001621"
     CDS             2352..2708
                     /locus_tag="SCL80_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEG1107491.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYKEILEEYLR
                     RTANDKKTRSIQHGFI"
     gene            2695..2913
                     /locus_tag="SCL80_001622"
     CDS             2695..2913
                     /locus_tag="SCL80_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107492.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            2910..3329
                     /locus_tag="SCL80_001623"
     CDS             2910..3329
                     /locus_tag="SCL80_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1107493.1"
                     /translation="MIPKFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIDLMQSTGMKDKNGVDIFEGDIVLVSVRNGFDYLDNKVCIVKNSIDYSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVTGNIYENSELLEEQR"
     gene            3326..3883
                     /locus_tag="SCL80_001624"
     CDS             3326..3883
                     /locus_tag="SCL80_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1107494.1"
                     /translation="MNKQEAIKKLESIKAIGNDAIAACYNESINSGITLMKKIDEPQK
                     PVIPQLVAGWLEKSTDPFTKAEKIAYLIKSKDGDSYYFCDWFVRDGIVTQEQGEELLA
                     WATRQSYETLLSLYNGYEVEKEPLWAIKNADGNYLTKCALWGKDGVNYSFECNPSHRL
                     LFTDKATADAAALLVNGTVEEVVER"
     gene            3880..4176
                     /locus_tag="SCL80_001625"
     CDS             3880..4176
                     /locus_tag="SCL80_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107495.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKSKFKVGDMVRCEA
                     EEFVYPFRGYVKKILSNSAIIRIENTMKCDRSTAKHKHYLAVARLVDMEVIKSK"
     gene            4253..4666
                     /locus_tag="SCL80_001626"
     CDS             4253..4666
                     /locus_tag="SCL80_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1107496.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCVPDSFSNYRI
                     SREVGYSERSIQRMKSEALIEFAEAYKHGRIIAYK"
     gene            5012..5386
                     /locus_tag="SCL80_001627"
     CDS             5012..5386
                     /locus_tag="SCL80_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107497.1"
                     /translation="MEDKMYLRQKVFTKKDIENQINGFIQKLENANSYLINKELNKAY
                     DQWKEVYDELKLIQNETKLVRTEKKNENSFFFDGYAILMLETVAKQNIKAPKKELSDN
                     IDNALAELRYYVMQIKDVRITR"
     gene            5423..5590
                     /locus_tag="SCL80_001628"
     CDS             5423..5590
                     /locus_tag="SCL80_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107498.1"
                     /translation="MYRPQYLEQKYEVITVQNGNGEIVRKYRRPIKSDTYKRKESNEV
                     IPLYGKRIAKH"
     gene            5616..5960
                     /locus_tag="SCL80_001629"
     CDS             5616..5960
                     /locus_tag="SCL80_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEG1107499.1"
                     /translation="MNEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            5965..6246
                     /locus_tag="SCL80_001630"
     CDS             5965..6246
                     /locus_tag="SCL80_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107500.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            6349..6663
                     /locus_tag="SCL80_001631"
     CDS             6349..6663
                     /locus_tag="SCL80_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEG1107501.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            6641..8335
                     /locus_tag="SCL80_001632"
     CDS             6641..8335
                     /locus_tag="SCL80_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEG1107502.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNANLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            8355..9533
                     /locus_tag="SCL80_001633"
     CDS             8355..9533
                     /locus_tag="SCL80_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEG1107503.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            9496..10182
                     /locus_tag="SCL80_001634"
     CDS             9496..10182
                     /locus_tag="SCL80_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEG1107504.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            10182..11342
                     /locus_tag="SCL80_001635"
     CDS             10182..11342
                     /locus_tag="SCL80_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEG1107505.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGRLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            11352..12227
                     /locus_tag="SCL80_001636"
     CDS             11352..12227
                     /locus_tag="SCL80_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107506.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIQIWTAEMWARLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            12224..12535
                     /locus_tag="SCL80_001637"
     CDS             12224..12535
                     /locus_tag="SCL80_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107507.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            12525..12878
                     /locus_tag="SCL80_001638"
     CDS             12525..12878
                     /locus_tag="SCL80_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEG1107508.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            12868..13269
                     /locus_tag="SCL80_001639"
     CDS             12868..13269
                     /locus_tag="SCL80_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107509.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            13262..13666
                     /locus_tag="SCL80_001640"
     CDS             13262..13666
                     /locus_tag="SCL80_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107510.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            13678..14286
                     /locus_tag="SCL80_001641"
     CDS             13678..14286
                     /locus_tag="SCL80_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1107511.1"
                     /translation="MAKTKTVVTTFDNVSIKRIAFNFKNAGNAIATNCNGQLDGETEM
                     QTVVKKCGATEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDGRIKPGIYSYGPDSV
                     GEDFSLAAEIVDDFEENRKLVGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFG
                     KFYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            14306..14668
                     /locus_tag="SCL80_001642"
     CDS             14306..14668
                     /locus_tag="SCL80_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107512.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            14671..14853
                     /locus_tag="SCL80_001643"
     CDS             14671..14853
                     /locus_tag="SCL80_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107513.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            14870..18289
                     /locus_tag="SCL80_001644"
     CDS             14870..18289
                     /locus_tag="SCL80_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEG1107514.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNTLAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     VTAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITAIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQEIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWISIAWNGIVTTFHTIFDPVVAWWNGIWTSISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWSSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSVFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGESGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRTQAVQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            18340..19077
                     /locus_tag="SCL80_001645"
     CDS             18340..19077
                     /locus_tag="SCL80_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1107515.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVAGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINHFNCTGLSKIQIYRNGLR"
     gene            19087..21378
                     /locus_tag="SCL80_001646"
     CDS             19087..21378
                     /locus_tag="SCL80_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEG1107516.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITATQAWQNAKALAKPAIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLHHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLINDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGTDYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDDGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            21402..>21988
                     /locus_tag="SCL80_001647"
     CDS             21402..>21988
                     /locus_tag="SCL80_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107517.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDSVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIQKYNETFDAFMAEIEGRVDSLNKQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSI"
BASE COUNT         7933 a         3246 c         4705 g         6104 t
ORIGIN      
        1 aaataagcct caaagacgaa tcgaatacaa ccgtgagtga tgccaatatc ttagcgaata
       61 tcatcctaga tttcatcttt gaacatcata ttcctttcaa agaaggatat gagattttac
      121 cagcgaatca agagtattac ttctacaaat gcattacaaa aagagtctgc tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgcaaag
      241 aagttgatca tgcagagtac acatttgcag cactctgcag aatccatcac acagagaagc
      301 acaaaatagg tgtgattaat ttcaaaaata aatatcaaat caaagggatc aaattaaacc
      361 agaaaacaat caaaaagtta aatattggag ggtaaaaatg acagaacatc gaagttatta
      421 tgcgattata ccagccaacg taaggtacga caaaagactt aaaccaaata ctaagttgtt
      481 atacggagag ataacggcct tgtgtaatga aagaggcttt tgttgggcag gcaatgagta
      541 ctttgcagat ttatatggtg tgaataaaga gaccatatcg cgatgggtaa gtgatttgat
      601 taagtttgga tacttgaatc gggaaatcat ttacaaagag ggtaccaatc aaataatcaa
      661 taggtaccta cgaattaatc aataccctat tgacgaaaaa cgcaataccc ctattgacga
      721 aaaagtcaaa gataataata catctattaa taatacattt aataatacaa aagaatatat
      781 aagagagtta ccgccttcga aaaaatcgaa ggctaagccc gtccgtcata aatacggaga
      841 gtataaaaat gttcttttgt cagatgagca gatggagaaa ctcaaaacag aattccctaa
      901 tgattaccaa gagcgaatcg aacgactgtc agagtattgt gaatcatctg gtaagactta
      961 taaaaactat ttggcaacta ttcgaagttg ggcaaggaaa gaaaaaagtg aacctaagaa
     1021 cgcaagcagt ggatacaagc gcacaggaag acgagagaag cttcctgaat gggcaatcga
     1081 ccaagaagcc tatctcaaga aaaaagcgct agaacgagct aatagacaat caaaagcacc
     1141 attctaagag gtggaaaaat gaagatcgat tatctagaac taattaatga aatagcgaat
     1201 tataaaaagg gcgaggaatt agacgtcctg agagacgtat atgatcaact cgaagaagct
     1261 ggaattgaag gaattaagaa tgatcgttcg agttggagta agctcagata ctatttcgca
     1321 ctttatatcg atacaacaca attaagaaat ttagcatata ccaaattact atttgttgat
     1381 tgtgttaaag gcttgcaaaa acatcttaat gaacttgagc aggtgtaatc agatggacct
     1441 aaagacattt acagcacaga ttgaactaat gcatcaagaa gctttaagac aaagtgtatc
     1501 gtacgaagac aagtggctca acacgttcca tggcggacgt gagagcgcac ttgatcaagt
     1561 actcaaatta ctgaaaggag aatgtcggga tggataagaa agcggcaatg cagcgaatta
     1621 tcgaattgac ttattcagaa gattggcaaa atgacaaaga agctgcttca gaagtgatga
     1681 ggcttggaag agcgatgtgg gcagacaaga gcaacaagcc aagaccacga aaaatcgcaa
     1741 tttggcacgg tgacaaactt ctagtgatag ggacagctga acagttagca agtctcacag
     1801 gcttgcacga gaaaatcgtg aggaaaagag caaggtgtgg ctacacagac gctaagaaga
     1861 gaacgtttag atacgtggag gaatcatcat gacaacagaa gaagtgattc aaatgcgtat
     1921 tcgaagcctt cagcgtgaga ttgacgatct ggaacgaaca aaggcagtga tggtcaatga
     1981 aacggcgaga aaggcaatcg atttgcacat agagaattta agaagggaaa tccatcgatt
     2041 ggaggaatga gcgtggataa gaaagcagca atgaaaagaa ttgctgaatt aaccaagtca
     2101 gaatcttggc aagaagacaa agaaatagtt gcagaagtcc aaaagctcgg taaatcaatg
     2161 tggactgaaa agcccaaacg gagaacgccg agaaaaattg caatctggca tgatgaccga
     2221 attttagtaa caggtactgc tgaacagtta tctgaaatta ctggattaag caaaaacatt
     2281 atctgggata gagctaggag cttatggatt gattcaaaag gacgacagtt taggtatgtg
     2341 gaggagagat aatggatctc attacacaat acagtgacat catcctcaag aaaatcatga
     2401 tgaagattca gaaagataaa aaatcaaaag aacgagctga attagttaag ttagaaatgg
     2461 ctgaaacagg agcaggagtg cgaagtagca ggcattggaa agcagcagca aacattgaat
     2521 tttattacaa cgaaattcaa aaagggttcg atcagatgcg tgagctggat cggcaaacaa
     2581 attggagcaa gaaacttcat caagatcgtt tcaaatttgt agagaagtat aaagaaatat
     2641 tagaagagta tttgaggagg acagcaaatg ataaaaaaac tcgttcaatt cagcatggat
     2701 ttatatgata tcgaatcagg agcaacacta tctgtggaat cggaccatct aatcataaat
     2761 tttggtggaa agcgccagat tattttgtgg gtagttgatg atgtactgtt tccagaaatt
     2821 gttcatgatt tcgaagaatc aaaagcggtt gagtttgaaa tagtgaaaaa agtaatggaa
     2881 ttgattgaaa aatacgagga ggacagcgaa tgataccgaa gtttagagcg tgggataaac
     2941 gaaagaacgt aatgagagat gtagccgtct tgcattttac taaaaacggc aaaacaaact
     3001 ttattgaata ttggataaat cctaccgaat tgaaatcata tcacgtgcga aacatcgacc
     3061 tcatgcaatc cacaggaatg aaagataaga atggtgtgga tatatttgaa ggggatatag
     3121 tattagtcag cgtgcgaaat ggcttcgatt acttagataa taaagtctgt attgtcaaaa
     3181 attcaataga ttattccgga ttagtttgtg ccactgttga tgaagactta gagtatcaaa
     3241 tttttaacac agagctgttt gaagaataca cgtacgaagt caccggaaat atatacgaga
     3301 atagcgagtt attggaggaa cagcgatgaa taaacaggaa gcaatcaaaa agttagaaag
     3361 tattaaagcg ataggaaatg atgcaatagc tgcttgctat aacgagagta taaattcagg
     3421 tattacgtta atgaaaaaaa tagacgaacc gcagaaacca gttattccac aactcgtggc
     3481 cggttggctt gagaaatcta cggacccttt tacaaaagct gaaaaaatag cgtatttaat
     3541 caaatctaaa gatggtgatt catattattt ctgtgattgg tttgtacgag atggcatagt
     3601 gacgcaagag caaggagaag aattactcgc ttgggcaaca agacaatcat atgaaacact
     3661 attgagccta tacaacggct acgaggttga gaaagagccg ttatgggcaa taaagaatgc
     3721 cgatggaaac tatcttacta aatgtgcttt atggggaaaa gatggagtaa attatagttt
     3781 tgaatgcaat ccatctcatc gattgctttt cactgataaa gcaacagcgg atgctgcagc
     3841 attgttggtg aatggaacag tggaagaggt ggtagaaaga tgaaactaaa agacggattt
     3901 tacgctagca gtcacggtat cggcggttta atgctagata tgccgacaaa gaaccctaaa
     3961 acacgcaaga aatcaaaatt caaagtcggt gacatggtcc gctgtgaagc agaagagttc
     4021 gtttatccgt tcagaggata cgttaaaaag atactgtcaa actcagcaat cattcgtatt
     4081 gaaaatacaa tgaagtgtga tcgttcgact gcaaaacaca agcattattt agctgtagcg
     4141 agattagttg acatggaagt aatcaagagc aaataaaaaa agccggatcg ctccgactga
     4201 ttcaataaat ccaacacatt tattatatca cataaaggag cggtttgact tgatgcaatt
     4261 gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc tgaagaactt
     4321 tcggcgtttg gagcggatgg caggtcgctc tttgatagat attaagtcgc cgattattac
     4381 ggatatgccg aaggcaccaa agcacggcaa taaggcagaa gacgcgatca ttcagatgat
     4441 ggatatagaa gcggagagag atgcgattct agcggctttg atggctctta gtctaattag
     4501 tcgccagata ctctactaca gcttctgtgt gccagatagc ttctcaaact acaggattag
     4561 ccgtgaagtg ggttattcag aaagaagtat acaacggatg aagtcggaag ctctaataga
     4621 gtttgcagaa gcatataaac acggaagaat aattgcttat aaataatttg gcggtttttt
     4681 ggcggaatga tggcggtttt tagctattta ccagtgatat tatggtagtg tcgaaagatt
     4741 agtgataggt ctaagacaaa ataaaatgta agggaggaaa tctccctcat cgtttaatta
     4801 agcttcgata gacagcagca aataaactaa aggatgtggg ggtcagctcc tacagttagt
     4861 tcatgtgttg ctgtctatta attgcaactc tttcggtttt attgagtatt tactcaactt
     4921 aaaaaaactg ttgtttgtag atgtaagctg ttcctatttg tacatagtaa aaaatgatat
     4981 aataaaaata ttacgaaagg ggtgagagtg catggaagac aaaatgtatt taagacaaaa
     5041 agttttcaca aaaaaagata ttgaaaatca aattaatgga tttatacaga aattagaaaa
     5101 tgcaaattct tatttgataa ataaggaact aaataaagct tatgaccagt ggaaagaagt
     5161 atatgatgag ttgaaattga ttcaaaatga aacaaaatta gtacgaacgg agaaaaagaa
     5221 tgaaaatagt ttcttttttg atggatatgc tatattgatg ttggaaactg tagcaaaaca
     5281 aaatataaaa gctcccaaaa aagaactatc tgacaatatt gataacgcat tagccgagtt
     5341 aaggtattac gttatgcaaa taaaagatgt taggatcact cgttgagtgg tctttttatt
     5401 ttgcacaaag gaggtaacaa caatgtatag accacaatac ttagaacaga agtatgaagt
     5461 aatcactgtg caaaatggta acggagagat agtacgaaag tatagaagac caataaagag
     5521 cgatacatat aaacgaaagg aaagcaatga agttattcca ttgtatggca aaagaatagc
     5581 taagcattaa ataagattgt gaaaggagac gggacatgaa cgaggaattc tatagatggc
     5641 tattacagtt gataagagaa gatcgtttgg ttaagttcta tcagtctcct aaatggcgca
     5701 ggcttagaga gaaagcgatg aaacgagatc actatgaatg ccaagagtgt agaagactag
     5761 gtaagtatca tagagtagag aacgttcatc atataaagga agtcaaggat agacctgact
     5821 tagctttaga tttagataat cttatttgtt tatgtgttga acatcataat gaagttcatg
     5881 gcagatatct tacagcattg gataaacaag agaagaagat agaaagcttt gctaacttcg
     5941 atgcaagtga aaggtggtaa gtgcatgatc atcaatgata atggcagaga gtatgataca
     6001 gaaaagattg aagagtattc atcttatact cagggattaa ttaaacgttt gatatacgtt
     6061 cgctatgtag gtattaggga tctgttatca gataactgtt gtagtaaata caaagtgaat
     6121 caagtaagag aagcgttgaa taaagataat aacgtcgaaa gaataaaaaa tgtttttgga
     6181 tatagtattg aagagattaa ttattacatt gacttcgctg aagctttcat tccgatggtg
     6241 agataacccc cccttaaaat aaatcgcaaa ttttttgggg gtgatgaaac ggagggggct
     6301 gtcaggaaaa gagatttttt cgaactttat catgaaagga gggctaaaat gtttaaaaac
     6361 gaattgtctc aaaatcgcta cagagaaaaa ttacgccgct ctttaataag ccaattggaa
     6421 agtcagaaaa caaatattga gccattctta gataatgttg atcgttatat cagtttatgg
     6481 gaaacggcga tatcactgga agaagatata tccgagaacg gcattagact ggagaatggt
     6541 aaaaagaatg aatcagtagc gttgcttgtt tctgtcaaca aacaaatggg attgatgttg
     6601 gataaacttg ccattactcc tgaattggta ggtgaagcaa atgaatcaat tcctgagtta
     6661 taagcatatt gaaaattggt tcaaagctat agaagaaggc actatcaagg tatgcaaaga
     6721 gcaattattg ctaaaaaatt atctagaaga aagagtcttt actagagaag atatttactt
     6781 cgataagcag atggtagagg attcaatcaa tataccagca caatactttc cattcgaatt
     6841 aattccgtgg gaaaaatttc tacaatgttt tatttatggt gttcgatgga aaaaagataa
     6901 aacactagtg ttcaatagat atctttcatt aatgggacgt ggtaatggta aaactggttt
     6961 tgcttcttgg aacaacttct ttctactaac cgctaaacac ggtattaaaa attatgatat
     7021 tgatatctat gccaataatg aaagccaagc aaagactagt tttgatgatg tatttaaagt
     7081 aattaaagat catcctgatt tagataaaaa agtatttaaa gctacgaagg aagttattca
     7141 aaatatcgct acaaacagca aacttcgtta taacacggca aatgctagaa caaaagatgg
     7201 gaagcgacca ggtgcaaacc gctttgatga aattcacgaa aatgaagatt attcaatgat
     7261 aaatgtggct acttctggtg gtggtaaaat tcgagattat agagaatttt atgatacaac
     7321 taatggtcat gttcgtggtg gtccgcttga tgacattata gaagaatcaa aaatgattct
     7381 ttctggagaa cttggaattg acaaggatgg agcagaattt tctagtttgt ttccatttat
     7441 ttgtcgcttg gataacgata atgaagttga tgatcccgac atgtgggaaa aagcttgtcc
     7501 aactattaat tacaatgcaa atttaaaacg gaaaatgttt caagaatact ctcaaatgca
     7561 acgtaatgct ggtttaagac ttacgttcat gaccaaacga atgaacagac ctatggaaga
     7621 tacacgattt gctgttgctt catatgatga tgttctgcat acgaaagaaa aagaatttcc
     7681 tgaaaaaatg gatgaagtga taggaacagt cgattttgct gatagacgag attttgccag
     7741 cgttgggttg ctaggaaaat acgataaaga tgtttatttt acacaacata cttttatcca
     7801 cgaatcagcc cttcgattac aaaacatcaa acgagaggtt atagatattt ctatagatca
     7861 aggaaaatca cagatcgttc atggaaaaaa tatagaagct gattatattg taggttggtt
     7921 tcttgaaatg agtaataaat attatattaa aaaaatcgct atggatatgt accgtgcaaa
     7981 aatattgaag cccgctttag aagaagcagg ttttactgtg gaaattgttc gaagcggatc
     8041 tgttacacat ggtatgttaa aagatctggt tgatgacctt tttattaatc aacgtttatt
     8101 ttttggtgac gatgcgatta tgcgttggta ttgcatgaat gtatatgaag agcatatttc
     8161 taatggaaat atacgctatg aaaaaataga acctgaaact agaaaaacgg atggcttttt
     8221 ttcattcctt catggtttga attttttaga tgatatttat gattctgctc ctgtaacagt
     8281 cacaaatagc tcagtagaaa atacaggaac tggatttact cctctagtat tctaacttga
     8341 aaggaggtga gaaagtgggg atttttcaaa aggcggtagg atacttcaca aaaaaagcaa
     8401 cggttccttt agaagaatac ttttgtaaat tgcaagttga ttttgtgtat cgaaaatttg
     8461 cgattgaaac ttgtattgat ttgattgcaa atgcgatgag taaagcggaa ttcaagtcat
     8521 atgaagatgg aaaaaataaa aagaatgatc tttactatag gctgaatgta gctcctaata
     8581 agaaaaataa tgcaacagaa tttagaaaaa aactgatcag gagattaata ttctacaatg
     8641 aagtattgat cgtttctccg tctaataatt ctagcgaaat atttattgcg gatagttggg
     8701 atgtcacaga atatgcattg aaagatgatg tgttttctca agtgcaaatt aacaacatag
     8761 tccttgatag agaatttcta gaaagtgatg ttatctatat aaaatacgca gatcaacaaa
     8821 ttaggcaact agtcgatgcg tattatcaag cgtatgggaa actcatttct agtgccatga
     8881 atgtttacaa gcgctctaac gctcgtagat acgtactgaa agggaattta ttccgaccgc
     8941 aagacaatac aacacaagat caaatcaata aaatgatgac atcacaattt aaggctttta
     9001 tggaagctga taatgcaggt gcggtatttc aattacaaaa tgagtacaca ttagaagatt
     9061 tcagcggaaa cttccaaagc aattcaagag atataaaaaa cttaatagac gacatctttg
     9121 agatgacagc agcagcgttt cacgttccga aaaacctact aaagggagac atgagtgggt
     9181 tatcggatca agtggacgct tttttaatgt tcgaaatcat accgattgct gaacttattc
     9241 aggatgcgtt taacgctagt ctctatgaag tagaagaata cttgtcaggg aattttgtac
     9301 gtgtggatac aactatgatc aagattacta gcttcaaaga tttggttgac gctattgatg
     9361 taggcattag aaatggagta tttacaatca acgaaggaag agaacgcgtt ggaaatgatc
     9421 gctctgataa ggcgatggca gatgaaatat ttataactaa aaacaatcaa caagtatcga
     9481 aaggaggtga ggcgaatgac gacaatgaaa acatttctag cagtaaagaa tgaaggcgca
     9541 gtaccgcaaa tttttattca gggatttatt ggttctagtt ggttctttga agggaatact
     9601 gacaagggaa tcaaaaatat tttggatagt ctaggtgatc aagaagaaat tgaagtagta
     9661 attaattcaa acggtggaga cgtatttcaa gggattgcta ttgggaactt acttaagtca
     9721 aataaagcaa aagttaacgt tgtgattaac ggcttagccg ctagtgctgc ttcaattatc
     9781 gcaatggctg gcgatactat aaaaatctac aacaatgcac aattgatgat tcaccgcgct
     9841 tccacatacg gagaaggaaa tgtcgatgac ttccgtacga ttgctgacca actggaatca
     9901 attgataaat cggtaaaggc ttcatataaa acacgattca atggcacaga tgaagcattg
     9961 caagaacttc ttgaaaaaga atcgtttatg gatgcagaaa cagctttgag ttatggattg
    10021 gtcgatgaaa ttatcgatgc agaaaatagc tcaggtactg aagctaaaaa agaacaaagc
    10081 gttgaagaaa ttttgaatga cgttaaagaa aaaagagcag aaaaaattgc tgcatttaca
    10141 gcagcattaa ataaaacatt tggacaagga gatgtaaaat aatgacagtt aaaaatttaa
    10201 aaggtgtaac agctgcaagc gaccaattga tgaaagcttt taaagatggt aacgaagaat
    10261 cttttagcgc agctatggta agcttatcta aggaaattca ggataaaatt ttagaagaag
    10321 caacagcaaa aaatcaagat caattagtat taatgaaccg tggtcagcgt gtattaacta
    10381 cacaagaaac aaaattctat aacgaagtag tgaaaaacga aggttttgca ggggtcgaag
    10441 aattagtgcc agctactgta tttgaacgtg tatttgaaga tttagaacaa tctcatccac
    10501 tattgcaaaa aattactttt gttaacacaa ctggtgtaac agaatggatt gtgtcacgtg
    10561 gagtcaatcc agcatggtgg ggtaaacttt gcgaagctgt taaaaaagtt ttagataatg
    10621 gctttgatgt aattaacatg aagcagttca agctatcagg ttatattcct gtatgtaagg
    10681 caatgcttga tttaggtcca gtatggttag atcgttatgt ccgtactgtt ttagtagaat
    10741 cattgagaat tgcattagaa caagcaattg ttgatggtac tggtaaagat atgccagtcg
    10801 gaatgatgcg tgacatgagc aaacaaacta gcggagaata tgctgaaaaa aaagcagaac
    10861 ctattacagc tttagatgct gtaactatgg gccgtttgat ggcgcgacta tcaaaattca
    10921 atatcgaagg tgtgaatgat ccgatttatc gtaatgtgaa tccttctgat gtggtcctaa
    10981 ttgtgaatcc aactgattac tggtctaaag tattcccagc taagactgta ctaactgcta
    11041 atggagaata cgtacaagta ttgccagtac cagtttcaga tttgcagtca acggctgtgc
    11101 cagaaggaaa agcagttatt ggggtagcct cagattactt catgggtgta ggatctacac
    11161 taaaaattga agcttcagat gaataccatt ttgttgaaga cgaacgcatt tatctagcta
    11221 aacaatatgc aaacggtcaa cctaaacgta acgatagttt cattgtatta gatattagcg
    11281 ctttgggaac tactactaca actacaaaac caacaaccac aacaactaca acacaagcgt
    11341 aggtgatcag aatgaagtat attctttgtc agccggcaat caatcggttt aaatgggagc
    11401 ttgaagtttg tttaactaat ctgaagaaac taggaatcaa agatatcgta ttgcttttca
    11461 gcagacacga tgatcagatt cctatttttt ttgagaagga atatggtgtt gaagttcatg
    11521 tgtacgatga tctgcgggac gacaaagagt atattccttc gattaaacca tatttatggt
    11581 ggaaatattt agaagaagat cattcgcgtg aggacgaccg atatttctat atcgattcgg
    11641 atgtcatttt caataaaaga attaatttgc gcaaattgcc ttctaaagat gatgtttggt
    11701 attgtagcga ctgctgtagt tatctaagtc ttgattatat tagaagctgt gaaaacggag
    11761 aaaatattct aaaagatatg gcaaacattg taaatgttac agtagaatct ttggaaacta
    11821 taaacactaa ttcaggaggc gcacagtggg ttattaaccg tcctaaagcg aattattgga
    11881 aaaaggttta tctggattct aatcggctat atcgctacct tagagggcaa aaaacaaata
    11941 tacaaatctg gacagccgag atgtgggcac ggctttggaa catgatgtat ttcaatattg
    12001 gtcctaaagt tcacgaggaa ttagactttt gttttgctac tgatccaata gaaaaagtta
    12061 aagaagtaaa aatcttacac aatgctggag taacaacaaa cgatgaagat ttatttttca
    12121 aagggagata cgtgacttct acgccttttg atgaagattt atcatttgta aacaagaaaa
    12181 aatgctctta cgcatatgtt aaagcaatta aggcggtggt tagatgacgc ctgaacaagt
    12241 gactgaagaa ttgctaatag ctgtgaagga taatatttac gttacctgga acgaagaaga
    12301 tgagtcaatt aaaaagatga tagctaaaaa tgctgtttat cttcaaagta aagtgagtac
    12361 aacactttct ttttctcctg aaagcttaga atacggattg ctaatcgaaa gatgtagata
    12421 cgactggaat cgtgctttag atgagtttga acaaaatttc gctagtgagt tattaggttt
    12481 cattcaacat tatgcgctac aagaatatat tgcaggtgat gtgaatggcg aataatcgta
    12541 gactcgaaga aacattcaac gatggttggt taaagatttt gacgcaaact accaaaagaa
    12601 atgaactagg aaaaaagatt ggtgtagaag atacagaaat cacttcttta aaatttagaa
    12661 atctttccat gagagatagt gatataacag ctatggatgc gatgggatcg aaattaacta
    12721 agaaagtaaa gactccattt catccaatcg ccaagaaatt taataaagat caatatttta
    12781 tcgtaatcga tagtatgcgt tacaacgtta tctatgccga ttacgataat ttttatatct
    12841 atttttatct tgaaagtgtg ggtgaatatg gtgattgata attctaaaga aaaagaacgt
    12901 ttaaataagc aaatttctgc tatcaaaact tccttagaag agcattttgg cctcaaactc
    12961 tttcaagact ccgtaggcga ggatgagcta cctgatgatt ttaattactt cattctcgaa
    13021 acaggagaaa tagaaatgat cactgagcca aaatatagcg tgggtcaaaa tctatatcta
    13081 actttctatt cagaaaatag agaagattta acaggagact cactagatat tatttcattg
    13141 attcaaaatc gttcgattcg ttttcagaga atggatccca atcatttaaa actagagaac
    13201 caagatcgct atatcgatca attggtattt acgtttagac gattattgaa gagtgattgt
    13261 catggctaaa aatagttggg agctaaaaat aaatggacat gatgaacttc ttgtgcggat
    13321 ggaacgctat tcaagcgaga gcgaacgact gattaacgaa gcattgaaat caaaaggttc
    13381 ggctattgca gtggatagga ttacagaaaa aattcctgtt tctgaagcag atttaagaag
    13441 agggcaccaa cacgcaaaaa atagtcgtcc acttaagact caatacatta atttgggttt
    13501 catcattaga cctacaagaa aatttgagta tttaaaatat cctgatttgg ggataggtac
    13561 ttctaaaaga aatcagccgg acgaatttat gagaagagga ttaggtcttg cacttgatcc
    13621 aattacagaa cttctgattc gtcaattcga taaattaaat aaataggggg aacaacaatg
    13681 gctaaaacaa aaactgtagt aacaacgttc gataacgtaa gtatcaaacg aattgctttt
    13741 aattttaaga acgcaggaaa tgcaatcgca acgaattgta atggacaatt agatggcgaa
    13801 acagaaatgc aaacggtggt taaaaaatgt ggagcgacag aagtaaaatc aaaatctaaa
    13861 ccaatcaata tgacggtaac aattactgca catgtaccga tggaagttta tcgacgtttc
    13921 aatgggttga aacaagatgg acgtattaaa ccaggcattt actcttacgg tcctgattcc
    13981 gtaggcgaag atttctcact tgctgcagag atcgtggatg acttcgaaga aaatagaaag
    14041 ttagttggta tgttagcatg cacttcgaat acaggattaa cattctctat tgaaaatggt
    14101 gcggatgaag tagctgcgtt agaactagaa acaaaagtta tgcaagatga atttggtaaa
    14161 ttttatcatg aagcaattgt tgcagaactt gaagaagact taacagatca atggatgacg
    14221 aatctatctg ctgatgtgat taaaaaaact tcagttgtga caactacggc cactcaatca
    14281 cagtaaaaaa aacggaggta gcgaaatgaa cgaagattac tcaaaaattg aactaaacga
    14341 tggaacaatt ttgaatttag aacctaaact gaatatcaag aaattattga tgatcaatag
    14401 agattttaac acagacgagt ttgcaaaaat gactgtggga aaaggatcca tggatatttc
    14461 tgttattcaa ggtgcaaagg ctgtgtatat tgcttaccgc caagcgaaca tgactgatta
    14521 tatttcattc gatgaattta tcgataaatg ggattttgat atggctactg ccagctatat
    14581 ttatcaattg atgatgttca aacaagcacg cgatgcttat caaaaagaat ttgaaaaagc
    14641 aaataaggaa aaaaagcttc aaaagtaaaa atgccaaagc tcttagttga aacgtgggtc
    14701 gatgtctatt cgatgttgac cgacgttttt tctatgcctt cagatttggt tttaagcgat
    14761 atctgtttag atgacatttt gcaaatggct tacaacaaga gtgcttatga aggatggaaa
    14821 aactatgcaa taaaccaatc tcagaaaaac taaagaaagg aggtaaaaaa tggctaaaaa
    14881 gagaacagaa gcagaagtaa ctttcatagc taacgatgac ggattgaaat ctacgttaaa
    14941 agaaatcagt gctgaattaa ctaaaaatag agcagaatta aaactagaac aagctcaatt
    15001 acaacagact ggttctgaat cagacaagtt aggaagtaaa ttatcttctt tagaaaagca
    15061 gtatgaatta caaagccaaa aagttgaagt aactagccaa cgtttagcca atgccaaaaa
    15121 atattatgga gaaaattcca ccgaagttca gaaacttgag agagaactga ttaaccaaca
    15181 aacagcgcaa caacgtttgt caaacgaaat tgataaaacg agtaatacac tagctcaagc
    15241 aaaaggcgaa atacagacgt acgagtctac aatgcaacag ttggatagtg aacaaaaaaa
    15301 tgttcaagct agtgcttctc tgattgaatc cgaatacaaa aaatggcaag taactgctgg
    15361 tcaatcagct tctgaatccg agaaattagc gaaagcccaa gaatatgttt ctcaacaatc
    15421 tgaaaatgca gagaaaacga tagatatcct gagacgacag ttagaagcta cacagtctga
    15481 gtttggcgct acatccacag aagcaatgca gatggaggcg aagcttaatg atgctgaacg
    15541 tgaatttgaa gagttaggac aagctgctaa aaatgtagat acaactaact tggacgatat
    15601 cggaagcaaa atagatatga ataatttaat ggaagcttct gacgttttaa gcgacattgg
    15661 cgataagctt acagaattag ggaaacaagc agtggactct gctaacagtg taggtagttc
    15721 ccagagtaaa atacaagcta attttggttt gactaaacaa gaggctgaag aattaacgaa
    15781 tgtagccaga gacatttatt ataaaggttt tggagaatcg ttagatcagt ccacagatgc
    15841 attgattttg gtaaagcgta atttaggcga tttaaataat caagatttac aaaatattac
    15901 ggaacaagct atggtcctag aaaacaccat gggcgctgat atggatgaaa cgttacgtgg
    15961 tgtaaatggc ttaatggtca acttcggatt gagcgctcaa gatgcaatgg atttaatggt
    16021 ttcgggtact caaaacggtt tagataaaac gcacgaatta ggcgacaata tggcagaata
    16081 tagccaatta tggagtcaaa tgggatattc agctgatgaa acgttcggaa tgcttcaaaa
    16141 tggtttagat gcgggtgctt ataaccttga taaagtcaat gacttagtta aggaaatggg
    16201 aatatcgtta acagatggtc gatttgagca aaacatggat atgtttagtg aaagtactag
    16261 aaaagctttt gaagagtgga aaaatggtgg aggaacacaa aaagacgtta ttaattccat
    16321 gattcaagat tttagcaata tggatggtca atacgaccaa ttaaataaag cttctacaat
    16381 ttggtctgca cttggcgaag ataacgcgat gaaagttgtc caatctttga ctgatgttaa
    16441 ccatacattt gatgatgtta gtggatctgc acaaaaaatg aatgaagatt ctactactcc
    16501 gttacaagaa ttaaacggaa aaatagctga attaaaggat tcattagctc ctataggcaa
    16561 cacaatcata gatgcactcg aaccagtaat tgattttcta ggaaagatgg ctgatgcgtt
    16621 taataatctt ccacaaccag tacaggatta tgccgtagcg attggcggat tgactgctgc
    16681 atttacttta ttaatgccaa taatagttgg cttcatggct ctaggtggtc ctactacatt
    16741 aataatagga gcagttatta ctgctattgc tggagttata gcaattataa aaaactgggg
    16801 cgcaattact gactggttta agggaatatg gagtaaattc actgattggt tgggtggtac
    16861 ttgggaaagc ataaaagaag gtgcctcatc agtttgggat ggagtcaaag aaacctggtc
    16921 tggatttgta gattgggttc aagaaatttg gcaaggagtt tctgattggt ttggagagtt
    16981 atggagcgga ttagttgaag gagcttccaa catctggcaa ggagtccaag agacttggca
    17041 agcattcgtt gattgggttt caaatatttg gaacggagtc aaagaagtat ggtcgattat
    17101 ttgggcagac attgtaggaa ttgttcaaat accatggaca ttaataacgt cattgattca
    17161 agctggtatt aatattatcg tgggtatctt tgatgtagct ggacagttat taggcgcagc
    17221 ttggcaagct gtttggacac ctatttctga tttccttaaa aatacttggg atactatgac
    17281 acaatggata agcatcgctt ggaatggaat tgtaactaca ttccatacta tatttgatcc
    17341 agtagtggca tggtggaatg gtatatggac atctattagt actacggctt caaatatttg
    17401 gaactcaatt agtgcaacag cttctagtat ttggaatagt atcaagaata caatcactag
    17461 cttggtacaa gcagctgcta cagtaattca aaatatttgg tcaactgtat ctagttggtt
    17521 aggtggaatt tggaattcaa tcagctctac agcatcaaat atctggagta gcgtgactag
    17581 tagtataagc aatgctataa acgcagctaa aagtgccatt caaagtgttt ggaatagtat
    17641 atcttcgtgg atcagcggaa tttggaacgg tatcaaaaac actgctttga atctttggaa
    17701 tggaattaca agcactatta gctctaaagt aaacgatgga aaaaatgcaa tttcaagcgg
    17761 ttggtccaat ctaacaggta ttgtttccga catattcaat aatgttaaaa gcacgatagc
    17821 taatatatgg gaaggtatca aaaagactgt tagcgctccg attgattgga ttagagataa
    17881 aatcagtagt gtctttgata atttgaatat ttctatacca catattccgt taccacattt
    17941 taaattgagc ggagaattca atccattgaa ggggaaaatc ccaacattgg gtgttgattg
    18001 gtatgcgaaa ggtagtgtgt ttaattctcc aaatattatc ggtgtaggtg aatcaggacc
    18061 tgaagcagtt ttacctttga aaagatctgt gctgcaagaa attggtgatc gtatcttgag
    18121 tagcacctca gtttcatcta ggacacaagc ggttcaacct gtgaacaatt acgaattcaa
    18181 tttcacaatt gatggtaacg cagatgaagt cactatgaag caaacaactc aacaaatcat
    18241 tgatagcatt acaaaagttc aaaatgataa tgcttcggca tggcgttaaa caggagagta
    18301 tttctcctgt ttttttagta ttaaaaagga tgtgaaaaaa tgactgattg tatacattct
    18361 ataatcgatg gatttcctga ttatttgcat aaattggctt tagctgaaag accaaccata
    18421 ccttctccaa aaagacagag agttgaaact tctgttttag gaaggttagg tggcttagtg
    18481 caagattact cgtttgaaga catgtcgttt acattgcact ataactattt agaggatgtg
    18541 gaagaccatc aagcgttcaa gcaatcgttt tatatcatgc gtcattggtt aaactatgca
    18601 aagaaattag aattctctga cgatcccaac gtctattacg ttatccagac tatcgatatt
    18661 ggggatgcag aaaacgatat tgttgaatgg ggagagttcg atgtaaatat tactgcgaaa
    18721 ccattcgcaa gagttcaaga agatgtacct ataaccgtag ataaaccaca gtcatttaac
    18781 ttgctgaata atagtttaga agaaagtttt ccaaagatta tcatcactcc ttcagctact
    18841 tcatgccagt tcatcttaaa tgattatgtg tttagtttcg aaggcttagt agcaggaact
    18901 gatgtagtca ttgatagtga tttgatgctt tgctacgaag agcaatcgga cggagatatt
    18961 ttagatcggt ccaacaaaat gaagaccatg caatatccga cattgcaagt ggatatcaat
    19021 cattttaatt gcactggttt gagcaaaata caaatttatc gtaacgggtt aaggtaggtg
    19081 aaatagatga tcgataattt aataactatt tacgataaaa atgacgcgaa taatttagct
    19141 gaacatttat atgatacgca aggtttaggc gctttgtcag actggttaac agctactgtt
    19201 agcaataaac taaacggagc cgagatattt cagggtactt atccaataag cggaactaat
    19261 gcagatttga ttgtagaagg acgtattatt cagtgttatg tagatgaaaa tcgagcaaaa
    19321 cagcgtctac ggatttatta tgcaaaaact tctgtaatag gcaatacgat agaagtaaaa
    19381 gctgaaccta ttttcaatga tataagaaaa tcggtgttga ataaatatga cagcggaaca
    19441 gaaaaaatca ctgctactca ggcatggcaa aacgcaaaag ctttagcgaa accagctatc
    19501 ccttcgcagt tttctttctc gtcattagta gatacgcttg ctaatgtgaa gatagaaaaa
    19561 gcgaattttt tagaattctt tggtggaaaa gagggatcta ttctagatcg atttcatggg
    19621 gaatttctta aagataataa cacattacat catgaaaaaa ggctaggtac ggatcataaa
    19681 atcaaagcga tttatactaa aaatttaact ggtcttgact tagagataga tgctcaaagc
    19741 gttttagttg gagtttatcc attcattagc agttcttcag aaggagaaga cgagatcact
    19801 ctaccagaag aagtcatttt cacggattac gtggatgatt atcctgctgg atatgtttct
    19861 tttgttgatt ttaaagacaa agcgactgat gtagccacat taagggaagc tgctaaagac
    19921 tggttgaaaa caaacataga taaacaaaaa ccacaagtga gtggttcgat tgaattagta
    19981 ccattgaggc atcaaagagg ctatgaaaaa tttgttgatc tagaaaaagt ttcgatgggt
    20041 gacggagtag atgtgtatca tccacagtta aaagtgaata tgtcagcgag aattgtggaa
    20101 tatacgttta atgttctaac caattcatac gataaattag ttgtaggaaa cgtcaaaaca
    20161 aacttcttag aaaacacaga gaataatgta agcaatttga ttaatgatgc cattgatcaa
    20221 ttgaaaaatg gtggcgaaat cagtgattta atcaatgata ttgtagatca tcaaactgat
    20281 atgattactg gtcaaaatgg tggttatgtt ttattagatc ctaaagaagc gcctagtcgt
    20341 attttgatta tggacacacc agataagaat accgcaagga atgttttaca aatcaacaat
    20401 gctggtattg gtttttctaa aactggcatt aatggaacat atgaaaccgc atggacatta
    20461 gatggcggat tcaatgcctc gtttattacg gctggtgaaa tagtaggaat tactattaga
    20521 ggtactacat taattagtga tggcactgat tatagaacaa gtattgctaa tggcaaaatg
    20581 acttggtact caaaaaaagt taacaaagat attatggagc tagaagcacg tgattatgta
    20641 agtgctgatg ccggtattgt atcatacacc atgaaaactg gtggtggttt catgattaga
    20701 aatccacagg gtaacttggt ttttagtacg tgggataatg gtaataacag accgtttcta
    20761 tcttttggtg cgcccaattt caggtatagc aatgctagtt atgtaacttc tggcgacggt
    20821 agttctttaa gcattaatgg tagtgcgggt aactcatggg aatttaaggt agctggtagg
    20881 actatgaaat ttactagtga tggtatgcta acgttgccag gttgtttttt tggttcatgg
    20941 gaagatggga aacttgctag gtttgaacaa tcaacggtac aagtatataa agattttact
    21001 gttagaggta ctaaaaactc aaccgtacca acagaacatt atggacaacg actattaaac
    21061 gcttatgaaa ctccagaata ttatttcgct gattatgggg aagccgttac aggtgatgat
    21121 ggtaaagttc gtgttgatat tgaccccatg tttgctgaga cagtaaatct aagtcgatat
    21181 atgacacatg tgacacctac agaactagtt ttgtgtgctg ttactcatga agatattgac
    21241 catttcatca ttgaaactag taagccaaac gtattagtta gatggaattt agtggcacac
    21301 cgtctagggt atgaagatat tagattaaaa gaggatacag catatgatag cacagtgctt
    21361 gaccaaaaac gtttttaaaa cgaagacaag gaggtatata aatggctagc agtttatata
    21421 atttggctct agatttcagc aaagaattaa actacaccaa agctattatg gctcgtcaag
    21481 gtgataaagg gattacagtg actgttaaac catttttaaa tggcttgcag atggatacga
    21541 gtggcggaac atttacttta aaaggaacaa cgccatctaa ccgttacgta gatagtgtgg
    21601 caactagtgt aactagtgaa gaagtcacgt tttctcttga tggcacattt atgagtgaag
    21661 caggatatta taaacactgc tacgtagaat atagaaaaga caatcaaatt ttaacaacgc
    21721 aagatatcat ttttttctca ctaggagtgt ctgacatttc gcaaggccaa gccgatgaat
    21781 acgtttcgca attggaagag ttgattcaaa agtataacga aacttttgat gcttttatgg
    21841 ctgaaatcga aggtagagtg gacagcttaa ataaacagat tactgattta actggtcaag
    21901 ctaaaacact acaagacaag ttagacgctc tgaaagaaga aatttctaaa ttaggtaact
    21961 tgcaggtgat gtacagtaac agcatcga
//
LOCUS       DARBEC010000048        21396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.048, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000048 DARBEC010000000
VERSION     DARBEC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.048"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            77..778
                     /locus_tag="SCL80_001648"
     CDS             77..778
                     /locus_tag="SCL80_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEG1107518.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(1387..2403)
                     /gene="tsaD"
                     /locus_tag="SCL80_001649"
     CDS             complement(1387..2403)
                     /gene="tsaD"
                     /locus_tag="SCL80_001649"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEG1107519.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(2400..2864)
                     /gene="rimI"
                     /locus_tag="SCL80_001650"
     CDS             complement(2400..2864)
                     /gene="rimI"
                     /locus_tag="SCL80_001650"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1107520.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(2871..3413)
                     /gene="rimI"
                     /locus_tag="SCL80_001651"
     CDS             complement(2871..3413)
                     /gene="rimI"
                     /locus_tag="SCL80_001651"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEG1107521.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(3397..4119)
                     /gene="tsaB"
                     /locus_tag="SCL80_001652"
     CDS             complement(3397..4119)
                     /gene="tsaB"
                     /locus_tag="SCL80_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEG1107522.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(4310..5290)
                     /locus_tag="SCL80_001653"
     CDS             complement(4310..5290)
                     /locus_tag="SCL80_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107523.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(5314..6798)
                     /gene="galT"
                     /locus_tag="SCL80_001654"
     CDS             complement(5314..6798)
                     /gene="galT"
                     /locus_tag="SCL80_001654"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEG1107524.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(6810..7799)
                     /gene="galE"
                     /locus_tag="SCL80_001655"
     CDS             complement(6810..7799)
                     /gene="galE"
                     /locus_tag="SCL80_001655"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEG1107525.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            8047..8214
                     /locus_tag="SCL80_001656"
     CDS             8047..8214
                     /locus_tag="SCL80_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEG1107526.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(8276..10087)
                     /locus_tag="SCL80_001657"
     CDS             complement(8276..10087)
                     /locus_tag="SCL80_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEG1107527.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(10084..10449)
                     /locus_tag="SCL80_001658"
     CDS             complement(10084..10449)
                     /locus_tag="SCL80_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEG1107528.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(10612..11007)
                     /locus_tag="SCL80_001659"
     CDS             complement(10612..11007)
                     /locus_tag="SCL80_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEG1107529.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(11025..11987)
                     /locus_tag="SCL80_001660"
     CDS             complement(11025..11987)
                     /locus_tag="SCL80_001660"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEG1107530.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(11987..12199)
                     /locus_tag="SCL80_001661"
     CDS             complement(11987..12199)
                     /locus_tag="SCL80_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEG1107531.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(12220..12918)
                     /locus_tag="SCL80_001662"
     CDS             complement(12220..12918)
                     /locus_tag="SCL80_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEG1107532.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(12938..13480)
                     /locus_tag="SCL80_001663"
     CDS             complement(12938..13480)
                     /locus_tag="SCL80_001663"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEG1107533.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(13612..14616)
                     /locus_tag="SCL80_001664"
     CDS             complement(13612..14616)
                     /locus_tag="SCL80_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1107534.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(14613..15602)
                     /locus_tag="SCL80_001665"
     CDS             complement(14613..15602)
                     /locus_tag="SCL80_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEG1107535.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(15599..16405)
                     /locus_tag="SCL80_001666"
     CDS             complement(15599..16405)
                     /locus_tag="SCL80_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107536.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            16571..17527
                     /locus_tag="SCL80_001667"
     CDS             16571..17527
                     /locus_tag="SCL80_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1107537.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(17604..18122)
                     /locus_tag="SCL80_001668"
     CDS             complement(17604..18122)
                     /locus_tag="SCL80_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1107538.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(18210..18359)
                     /gene="rpmG"
                     /locus_tag="SCL80_001669"
     CDS             complement(18210..18359)
                     /gene="rpmG"
                     /locus_tag="SCL80_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1107539.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            18587..19033
                     /locus_tag="SCL80_001670"
     CDS             18587..19033
                     /locus_tag="SCL80_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEG1107540.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(19228..21123)
                     /gene="asnB"
                     /locus_tag="SCL80_001671"
     CDS             complement(19228..21123)
                     /gene="asnB"
                     /locus_tag="SCL80_001671"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEG1107541.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT         6433 a         4547 c         3695 g         6721 t
ORIGIN      
        1 tggtatgtta aaactttaca aattctttaa aaaaagtgac aataaatata gtgaacaata
       61 aggagaggac tttttaatgc gtgttaataa aatcgatttt cacttttcaa aactcagata
      121 tcaacctgat tttgaacatt tctcggatga agagttccaa cagatcaagg aacatactgt
      181 tcttcgttct tataaaaaag gacagatcct tttcgacgat ggtgatccca gacaacggtt
      241 gtatatcttg accaaaggac tcgttcgttt agaacgttat gatgaaagtg caacttatta
      301 ctactatgac tatgtgaata caaataacct ttttcctatg tcaggaatgt ttggagaaga
      361 gacctacact tatacagctc aagcgatgac tgatattgaa acattctacc ttcctgtttc
      421 ctactatgaa aaattagcac gaaaaaatcc cgaacagttg atctatatga tacaaaaaat
      481 ttctaaagta gttgaaatac atgaattgcg tatccaaatc ggattgacct ctagcgcctt
      541 tgaccgggta aaacaaaatt tattcatttt gaaagaagaa ttgggcgtac caacaaaaga
      601 tggtcatatc accattcctt atccgatcac cttaaaagaa ttagcagtga atagcggcac
      661 gacacgtgaa actgctggac aagtaatcaa acaactacgt gattccgggc tgttagatta
      721 tgacaaaaag cgttttactt tctgtaagac agctactact ccagctatga gagaataacg
      781 cgaacaaaaa taagcagtga ccctgataaa tatcagggtc actgcttatt ttcctcccta
      841 aaacttctga aagatccggc agttttagat cactggacaa gactcaaaga ttttctatgt
      901 gtgtttcagt tatgtaatat tcactgtttg caaaaaatac tgttagatta actagatgaa
      961 gcgtcttgtc ctcatccgat ctagattatt cgatattcct cgacaataaa cgattactca
     1021 gtgagggcaa tcagaggaac ggataatgat aaataagctg taaaaaatag caaaataaca
     1081 ataatgatta aaagtatgta aaaaataggg ctttgacaaa aacgtgtcac agccctattt
     1141 cgattaaacg gtgtgtacct gtcaactctt cggtcacaag tcataatgtt cataaacatg
     1201 aggagctgtt ttttgaaatt gttccttgtg aatcagagtt aaacgacagg cttacgaact
     1261 cttgattaaa cggcgttcaa aagcttgcat acgcggtaat aagaacagcc aggtcaaaaa
     1321 catgagaagc cgtttttcct gggctgttct tattacttga tgcagggcag ctttttcaca
     1381 acctctttat tcagataaaa ccaagcttgg atcagcattc aactgatagc tagcaaaatt
     1441 tcttttttgc atttccacat gtgctgctgc tccaatcatt gcagcattat ctccacaaag
     1501 tcgaagaggg ggaatcacta gttctacctc agggagttta gctgccaaag catcctgcaa
     1561 tccttcacgc aacccttgat tggccgctac acctccagct ataatcagtt gcttgactgg
     1621 ataattttga caagctcgaa gtgttttagt aatcaataca tcgatcacac ttgcttgaaa
     1681 gctagctgct aaatcatttt tgtctagatc ttctccacgc tgttgtgcat tatgcaccaa
     1741 attgataaat gcacttttta atccgctaaa actgaaatca taattatctt catggatcat
     1801 cgctcgcgga aaatggtaat tatcttttcc ctgatgagct agctgatcga tttcttttcc
     1861 gcttgggtaa gaaagaccta atactcttcc aactttgtca taagcttctc ctgctgcgtc
     1921 atctcttgtc tccccaatga tttcatatga tccatcttct tgcatataga ctaattctgt
     1981 atgtcctccg ctaacaagaa gtgccatcaa tggaaactgg aatggtttaa ccagacgggc
     2041 tgcatagata tgccctgcca tatggtttac aggaataagc ggcaattggt gtgcccaagc
     2101 aaaagcctta gctgcagaaa taccaatcag caacgagccg acaagtcctg gtccataagt
     2161 gacagcgact gccgataaat cttctgccga cacaccagct tctaccagtg catcttccaa
     2221 gcataaggtg atttgttcta cgtgatggcg gctagctact tcaggtacta cgccaccaaa
     2281 acgtttatga ctgtttattt gagaagcaac gatgttagat aagatctctg ttccatttct
     2341 aacgaccgct acactcgttt catcacaact tgattcaatc gccagaatca attcttcatt
     2401 catttcatcg tctccgtttt taactttctg ctcataatga tcgcatcttc cgtcggattg
     2461 cgataatatg atttccgttt acctaatata cgaaactttt ctgattcata aagttttctt
     2521 gctgcttcat tagatagacg tacttctaaa aaaacatgca gcgaatcttt tatttgttcc
     2581 gtaatcagta attgtaacaa atgccgtgca tgcccttttc cttgttctga aactgcaaca
     2641 gcaatatttg tgatctcgac ttcatctatt actttcgagt atccaataaa gccacagatc
     2701 tggttatctt cagctaaaag cagataatca gagtgaggct gttggatgtc tgccaaaaac
     2761 tgcattttcg tccaaggtga accatgtgga taagcatttt cgctaatcat ccatacgcat
     2821 tctgccaagt acgcttcatc aaaatctttt tttctgcaaa tcaactaatc tcactcattt
     2881 tctaaaagtt ttatcatatc caacgcatct tcttttgttt cagtgtaata tctttttttg
     2941 atcgttgttg cttgaaaccc caatttccga taaagtcttt gtgcatcttg attactcata
     3001 cgcacttcta atgacatcgt ctcacagcga ttcattatag caaagttctc gatttcgtca
     3061 ataagcagcg aaccgattcg tttacgctga tatgccggtc gaacggcaat attcgtgata
     3121 tggcaatcta aaccgaacgc tcttgaaccg ataaaaccaa tcattcttcc attatcaaag
     3181 atacctagat atagatgagg aagcggtgac ttcaattctg atataaaggc actttttgtc
     3241 catggcaatt cgccattata gacttctcgt tcgacatcca gcagctcttt gatatcttct
     3301 gtagtcaatt ctcttagtac gtaacgacga tcgatttgtt cgactgtttt ttctgtataa
     3361 atcattttat cttgaaaaaa gcttctggca tacgctctaa attttttcaa cataactttc
     3421 gtctccaggt gtgtgcgtct ccaaccattt ttcttctgct tctactcgtt ttaaatagcg
     3481 aggaagaaaa gcttgtttct ctgcaactgg ttcaactaaa gcacctaatt gagccacagt
     3541 tacaccactt ggaatatccc atgctggaac tgtattaatc atcgcactag gcaatatttc
     3601 ctttatttca gagaagaatt tgtgacaatc actcccgacg aaataaatag cttcattcag
     3661 cttttgtagt tgtgtgaaca aatcagaagc agaaatatgt acatcttcaa gtacagtttc
     3721 taatctattt tctttccagc ggtatgcccc agcgtataca ttttttcttc gagcatcaaa
     3781 catgggcaca ataatattat ccacaccgat gcaattagcc gcgatggttt tcagactaga
     3841 tactcctact agctctttat ctaatgtata agcaatcgtt tttgctgtcg ttacacctat
     3901 acgaagccct gtataagaac cgggaccatc agaaaccgcg attcggtcaa tgtcttttgg
     3961 acttatttga agatctgcaa acaattggtc gattgcaggc atcagtgtta cactgtggtt
     4021 gcgtttgata tttgtttgta tttgtccaag aatttgttcg tcttctacca caccgatcgc
     4081 caaagtttgg tttgcagtat ctattcctaa tgtaatcata tatttgatcc tttctgctat
     4141 gacattctcg ccttccattg tagcatactt ttttattctg aggatgaaaa taccaaaggc
     4201 aaaactagta caataataca tgattatctg ctggcgggca aattttgccc cttcctgaaa
     4261 cacaaaaaag ctgcggtatc actccatttt cccacagctt ttccatcttt tatttcgttg
     4321 attctcgaat aatcaactct gtttccacaa taatctttct tgaaaccggt gctttggaat
     4381 tacttaattg aagaatcgtc tccactgcta gctctcccat ccattcggta taaactttta
     4441 cagtagaaag cgagggcgtt acatatttcg ctacactgac atcattgaag cccatcacag
     4501 aaatatcttc tggtaccact aaaccttttt cttgaatcgc tttcagtgcg ccaatcgcta
     4561 atgcatcatt agctgcaaaa agcgcatcag gaacatgcgg attcgttttt aaatattgct
     4621 tcactgcttg atatcctgac tcgacagaaa aatctgcttc gataattgga ccttcgtcgt
     4681 agcctttttc tgccaaaact tgttgaaatg ccataattct gggatctttt agccgctgat
     4741 ggtttttctt cgtatattct gttccagcaa gcatagcgat atgttgcgct ttatgggaga
     4801 caaagtgttc aatgactttt tgaacacttg acttaaagtc gacaactaga gagtcgattc
     4861 cttgagaagt gccgtcagaa tctaccaaaa gcaaggcatt ttgggatcct ttcatttcta
     4921 ataactgagc ttcgtcgaat tttcctagtg cgatcgttcc atctgccgga agatcactaa
     4981 tggaatccca cgtttctttc aataaagtaa cgtttgcttc ttctgccttt ttttcgattc
     5041 ctaggcgaat agataaataa taaagatctt caagctcttc cgtatcgtcg taccactgga
     5101 tcaggcgaag cgtttgctta gtatttgctg catttttacg atgtttcgta taatttaagc
     5161 gttctgctgc ttcaaaaatt ttttgctttg tggctaagcc aacggaaaga tcaggatcat
     5221 agttgagaac tcttgaaaca gtagcaggag aaactcctgc caactgcgca atgtctttaa
     5281 ttgttgccat ttgtctctcc tcctttttta cgtttataaa atcgcagtaa atcgttcaaa
     5341 cgctgcctgt cctttttcat ctcgcttgaa tactccagcg tcttccagta cacgtgcaaa
     5401 aacctgacct acagattttt gtacgacatc ggtgaccgta tctttcgtca aatcaggatg
     5461 ttcttttttt aattgttctg cccacggacg atgatattca gccatctggt tttcttctcc
     5521 taataagaaa tgctctactt ctactagttc tggtttcaaa cgaggtggta aaacagcaag
     5581 ccccattact tcgatcaacc caatgttctc ctttttgata tgttggacat ctttatgtgg
     5641 atgaaaaatc ccatcaggat gttcttctga aacattgttg tctcgaagga caaggtccaa
     5701 ctcaaagtaa ttgtcccttc ttcgtgcaat tggcgtaatc gtatggtgtg gtgtaccatc
     5761 tgctgaaaaa gctctgatag atactgtttc atccgaatat gctttccatt tttccaatac
     5821 tgagttagct gcttcaataa gcaagttttt ctctgttgat tgcaaacgga tcacagacat
     5881 cggccatttc acgactcctg cgatgacttg cggataatct tctagtttga taagtttttc
     5941 tatcggtgct tttgccatag gaaattcatg acgtcctgcc tgataatgat catgagaaag
     6001 aatcgatcct ccaacaattg gtaagtcagc attcgaacca acaaagtaat gaggcaagac
     6061 ttcagtaatc cgaagcagtc tagcaaatgt ttcctttgtg attttcatcg ggcggtgttg
     6121 ttctgataaa atgatcgaat gttcattgta ataagcataa ggggaatatt ggaatcccca
     6181 gctttcaccg tctagattca ttcgaataat ccggtgattg gtgcgggctg gatagttcaa
     6241 gcgacctcta tatccttcgt tttccatgca aagcatgcag gtaggatagt ctacttttac
     6301 cgcctcgcgt tctgctgcga tttgttttgg atctttttct ggcttagata aattgattgt
     6361 gatttccagt tcaccatact ccgactctgc cggaaatttg atgttttttg cgatggctct
     6421 tgttttgatg tagtcatttt tcttacttaa attaaagaaa tactcagttg cttcctctgg
     6481 gcttttcgaa tagtgctgtg caaagaatgc attgacgact gaaggtggcg gtgtgagaaa
     6541 atccattaat tgcgcttcaa aaatctcttg ttctgctggc aattctgaaa gaacaccatt
     6601 ttctcttgcc ttagcgacaa gctgatccaa taagtctaca gattcttcct taactggtct
     6661 tacttcaatg ctttctagag catcttctcc taccatccct aaaagacgat tctgtaaata
     6721 taagcggtcc atttccatcc atccgccagc ttcgatcgca agtgtggcaa aatcacaaat
     6781 cgtttgactg attgacatat ctcattcccc tattcttcat aaccatttgg atggctgaca
     6841 tgccaatccc atgctgtttt gataatcgct ttgatatctg tgtattcagg tttccagccc
     6901 agaatctctc tcgctttttc acttgaagcg actaatcggc taggatctcc agctcttctt
     6961 ggtgctattt ctgctggaat ttcttttcct gtcacttctc tagctgcttc tagcatttcc
     7021 ttgactgagt atcctttgct gctgcctaaa ttgaagaagt tgctttcatt tccttctttc
     7081 aagtattcca aagctaaaat atgtgcagca atcaaatctt ctacttgaac ataatctctg
     7141 atacatgttc catctggtgt atcataatcg tctccataaa cagccaaggc ttttctttga
     7201 ccaagagcta cttgtaaaat gattgggaca agatgcgttt ctggtgtatg atcttctccg
     7261 atagatgcat ctgcttttgc tccggctaca ttgaagtagc gcaaagcgac atagcgcatg
     7321 ccatatgctt ggtcacacca cttcatcatt ttttccatca tcaatttact ttcaccatac
     7381 ggatttttag gattagctgg tgtatcttct gtgattggag actctttcgg ctctccataa
     7441 gttgctgcag tagaagagaa tacgatttta ttcacgttga actcgtgcat gacttcaagt
     7501 aagatttgca taccgtatac gttgttgttg aaatacatca atggtttttc gacagattct
     7561 cctactaaag agcttgcagc aaaatggatt actccttcaa tcggttcctt ttcaaatacg
     7621 gaacgtagaa attctttatc ccggatgtct ccttcataaa aatgagcatc tggatgtact
     7681 gcttgtttgt gacctgtgag caaattatct accacagcta cttgataccc cttttgaact
     7741 agctggtcta cagcgtgcga accgatataa ccggctccgc ctagtactaa aattgtcata
     7801 aaaaacactc ctttgattag taaaattgtg tttgttgatt ctatataatt taaaaaacga
     7861 agtttacttt tatggtgaaa acaaacaaag taaaatttcg taaattcata tggtaaattt
     7921 ataataaaac atttagtaaa cattttcaag ggttttttgc aaaggtttac aaacaaacgt
     7981 aaaactagcc tttttttctc ttttgttcca taatagatat aacaaataat aaatgaggtg
     8041 gattcaatga aaaaatttgt atctggaata ttagtaggaa gtctggcaac agccgctgcc
     8101 gttgctggtt tggttgcttc tgtgaaaaaa acagtgatcg atccaattga cgaaaaagaa
     8161 gcaatgatcg aagaaaaccg taaaaaagca atgcgtaaac gcgtttctcg ttaataatta
     8221 tttaaaaata cgcatgaaga ctttctaaaa aacagataag tcatcatgcg tatttttatt
     8281 tgtcagtcaa tataagttgt gctattttat ctgcctgtct cagattccat ggatctgtat
     8341 aaggtctagc tactgctgca gttagccaat ccacaccttc aacaggtatt ccatagcaat
     8401 aaaagtcaga ctggatttcc cctgaagcat caatcacgcg attcgtacct cgctcaacat
     8461 ctatagcttc tgaatgataa cttcctgtat tatctgaaaa tgaaaaaggg cgaacaagtt
     8521 tgttttcctt caaatttttt atgacaggat tacttgaacg agaaaagctt gtttgtggca
     8581 atcgagcttc aatcaaatag cgactgctga tttctttatc ttgtagagaa ataacatagc
     8641 ggttgtgctt ttgctgaata tcaaatggcg gttcgatcaa atgaaaaata cccgcatcta
     8701 tcagtgctgc taattctttc gtccgaatga ttggcggacc gatcgttaaa aagctattta
     8761 gatgagtaaa ccatccccat agtatttctt tgtattcctt agcagaaaaa acgtgccatt
     8821 caatagcctg ccggattgga tcacgccagt cttttaatgc atcaaatgtt gaagcaaatg
     8881 ctcctgtgca attgcctttt tccgcttctt ttatctgccc ttcaataaat attcggctag
     8941 cttctgaaaa gggctcattc tgataggcaa gaggagtttc taaatacgac caatcccatc
     9001 tataagggat tagtgctgga tgctttccga gacactcctc tgaagagagg gagagaaaat
     9061 catgctcaaa atttttcttt ggaatcggca agtgatattc ttcgatgatt ttcttgtaat
     9121 aaaacaactc aacgtctttt ttcagatagt caaaaaatgt ttcgccagtc aaaaatcccg
     9181 actgatgcca ttgctccagg ttttcttttg ttagtaaccg tggccatgcc attgctccgc
     9241 cttgcttttg atttcttcct cgagggtaat aagggagccc ccttcttgat ccactgtata
     9301 caatcggttc cttgcctgat ggatgataga ccagttttcc cttttcgttc tcgaagcgcc
     9361 ctcctcttga tactgtaaac aatccgacat aatcaaaaaa acttaagcct agacctctta
     9421 aaaagacatt ttctttaggc ggtatcttcg aaagatctac atctgcagga ttagaaggaa
     9481 gctgatacag taaaacattt ttctttgcgt agtctaccca cactctttct tcttctgtct
     9541 catcattttc ccagtgaccg cttgctaaaa caagctgatc gatagaatag ctttgctgat
     9601 ctgtctgtaa aagataacca tcttttcttt tgcttacttt ttttactaaa gaataaacaa
     9661 actgaataga agctggaaat tcttcttgca actttgagaa aaaccagcgt tgatagagtc
     9721 cgtataaaca tcgagtagat tgctgatctt tttccagacg attgatttct tctataatta
     9781 gttgcttatt tttggttacc gtctgttcga tataatctgt agcaggtcct ttactccatt
     9841 gaaacaagtt gggtccctgt gttaccaatc ctccactgga caatgtttca tctgtaaaaa
     9901 gcgtcacttg ttgagaaaca gaattcatta ataactccgg tggctgattc aaccgccaaa
     9961 ctcgacctcc aggaccattg ggatcgaaaa gaaaaagttc aagagaatgt ctgtcctgat
    10021 tattatggag aatcctttct gctacactta atcctctagg tccggcacca atgatcccta
    10081 ttttcatttg catgcctcca tttcgataaa ttttttcaat aaaacagcat ggttctgatg
    10141 ttcatttttt gcaccataaa caaaagtaat gactggatgt tctttcataa gttgactgag
    10201 tttaaaaata gcttgcttct tttcttcttc gaaaatcaat tcttgctggt atttctttgc
    10261 aaatgaagca aatcgttctg gtacatggtc aaaagttttg cgtgcttcat tactaggtgc
    10321 aatttctttc aaccacagat ctaactgcgc tttttctttt gaaattcctc gtggccagaa
    10381 tctatccacg agtattctat agccatcctc tttttcagca tcaaggtaaa cacgttttaa
    10441 tttgatcata gttgtttcct tctttctacc ttttcgctta gcttatatat gtagataata
    10501 gcacaaaata aaaaaaatga acggcacaac tatccattgc gccgttcatg tgatcttggt
    10561 caaaatctaa tgattgattc ggtttttaaa gctgtcatac aatcatctgt tttatacttt
    10621 tttacttttg atttttccag accatccgtc atatccaccc ttaagtaaat aaatctcttg
    10681 ataacctgct ttacgtaatt tatttgctgt acggatgctc aaacttttct tttgatcgta
    10741 aatatagaca ggctggtctt ttcgaataga tcctaatgaa tctttcaaaa cagtgtaagg
    10801 gatgtttctt gcacctaaaa tatgcccagc atcaaacaca tctttttcgc gaacatcgat
    10861 tacttgtgct tttctcattc cttctcgaaa ttcttcttct gtcaaagtcg ttgctgaacg
    10921 tttggccatt actcgtagat agacttccca aaggatgatt gcaagcacaa tcaatagcaa
    10981 aatgatatta attacccata aaaccatgta atgcctccgt ttttttaagc aaatttcaag
    11041 gctaatgagg cagcaccaat gacaccagct tcattaccaa gctctgctaa tttgattttt
    11101 gttgattctc ttacttgcgg gaaagtaaat tctttaaaat atttctctac acggctacgt
    11161 aaaaattctc ctgctgccga tactcctcca cctaacacga tacttgacgg atttaatgta
    11221 ttccccaagt ttccgcaagc taaacctaag tagaagcata cgcgatcaac aaccatcaat
    11281 gcaaacggat cgtcttcttg agccatttca aagatatctt tacttgttac ttcttctccg
    11341 ttgtctagca ttgctttcag ttttgaatcc cctgcgtatt cttctgccaa gaaacgacct
    11401 aggcgtacca cacctgtagc gctagataca gtttctaaac atccgcgttt accgcaagta
    11461 cattcgaacc cttcaggatc cacagtgata tgtccgattt ctcctccagc tccgctcacg
    11521 ccgtgaatca aatttccgcc agcaacgata cctccgccaa cacctgttcc tagagtaacg
    11581 aatacaacgt ctggatcgtt ttctccagca cctttccatc gttcacctaa agcagcaaca
    11641 ttcgcgtcat tatcgatagc gaatttgatt cctgttcctg cctcgatcag ttttttcaca
    11701 aattgaactt ctgtccagtt taagttatac gcaccaataa ccgttccagc ttcgctgtcc
    11761 acgcttcctg gggtacccat gccgattccg ataaagtctt cggctttcat attatataaa
    11821 ttcaaacgat gattgattga ttcaatgatt tcaggaacga tatgcttacc atcatccaaa
    11881 atattggtat cgatgctcca tttttgctga atctctcctt ctggtgttaa aattgcaaat
    11941 tttgctgttg ttccacctaa atcaatccca ataatcttct tgtccattac aaatttcctt
    12001 tctttcggct ttcttcaatt cggtgctctc tttttaatat gcgccacgct gtcatgtatg
    12061 tatcatgatc aatcaaacgg ctatcataca gattacgcaa ttcgatagtc attaattcaa
    12121 tgtcatagat gcgtgcaccc acatagataa agatgccaaa ttttttgaac agctgctgta
    12181 catcatataa gctctccatc tttaagcact tccttcatat tatacaagta aaccatattt
    12241 ttttaaaccc agtaaaatac atattacaaa aagaaataca aaaactagtg tagaaagaat
    12301 cctttggtgt atcgaatagc ttgtctgttg tttcggtaga cccaaaacat tccctaacac
    12361 taatccgcca attagcccac cgacatgtcc ccaaacatct acagaggtat ccagaagacc
    12421 aaaaacaaat gtcaacacaa taaataaaag aaaatgtctg actagctgct ggatcgctgg
    12481 gttatgttta aaatgaaacc ctaagataaa caacgcaccg aacaaaccga atatcgaagt
    12541 acttgcacca ccagacagaa cattcgcttc gttaaaagca aagcttgctg tatttcccat
    12601 tatcccgctg aacatataaa tcaagaaaaa acgccaatgg ccatagattg cctcgatttg
    12661 ctgccccata aaatacagta tcagtgagtt cattacgaaa tgcatcagtc caaaatggat
    12721 gaagattggt gtcactaaac gccaccattc attgaagtga acaagataag gaccatacat
    12781 tccggcataa taagggatct caagtcgcgg aaataattcc atcatgagga agaccgctgt
    12841 ctgtatccct aaaaaagcat aggtccataa cggtcgtctt aaccatgcac gcaattttat
    12901 ttgctgttga taattcatga aaacctccca tttcatttta gtcaataaac aattgttgga
    12961 tcttctgatc aaagctctcc ggttgccatt gttcattcaa ttgttcacga aaaacaaggc
    13021 tacatgtttt tttatgaaaa ttatttaaat accggtcgta ataaccaccg ccaaaaccga
    13081 ttcgataacc ggctctattg tagataatac ctggtacaat gaggagatcg atcatctccg
    13141 gcaaatagag cttctctgat tgtggctcaa gaacaccaaa actcgaacgt acaagaacgg
    13201 actgttcatc catttcataa aacgccatct gacgtttcgg caaagtttta ggtacaacta
    13261 ttttcttttg ttctttttct gctcgttgga taaccggctg tgtatccagt tcaaattcat
    13321 tcgataaagt cgtccctatc acttttgctg acttccagta ctcgctggcg aatagctgtt
    13381 tatagattcg ctgttctttt tcctttttct tgccactttt ggccagattt ttcagctttt
    13441 cgattccttt ccggcgtaat cttactttca attggtccaa aaactcaccc ttccttctct
    13501 cataaaatgg tataggaaaa aaagaaaaat agcaatcgaa ctttcaaata aatgcataaa
    13561 tagaataaaa agaagaccta gaaatgattg gccttccttt tttaatgaat ttcaactgtt
    13621 cagcgcgtgt ttttgtctga taaaaagtgc atacagaaaa tatggcgcac caaaaagggc
    13681 aatcaaaatt cctgtaggta cttccccact tggtagaatc actctagcaa ccatatctgc
    13741 actaagcaga aaaatggaac cgaacaaacc gctcagaaca aaggtccagt tattttcttt
    13801 acgtacgact agcttcgcag catgaggagc tagtagtcct acaaaagata aactacctgt
    13861 caccgctaca ctaccgcttg ccaaagcaac agctaacaac aaaaggattt tttgactttt
    13921 agccacgtta aggcctaatc cttgcgcgcg ttcttgacca aatgatagca atacaatcct
    13981 ctgtgcataa agtcctccta gcggaattaa gataaccgcc catggaagca atgcccaaac
    14041 ataaggccag ctcgcacccc agattgatcc cgctaaccat gcattcacaa atccataatt
    14101 ttccttagat aattggatcg tgcatagcaa tgttaatgca gaaaggccgg cattaacggc
    14161 aataccggac aataagattc gattcatacc caaaaagcca tttcgatcat aggccgcaag
    14221 atagacacag caagcagcta aaacaccacc tagaaaggct acaactagca atagaaacgg
    14281 cagcgaatag tgcgaaaaga aacctaaata aagcatcact aaaaatcctg ctccggcatt
    14341 aatgcccaat atactagaat ctgccaattc atttcttgtt actccttgca agatataacc
    14401 agaaacgcca agacaagctc ctgctaataa actgatcagt aatcgaggaa ctcgaaattc
    14461 tctaacaatc agcgccaaag aagatgattc tttccctatg aacgtggcaa aaagctgatc
    14521 caggtaaata aaaggcgtac caatcattaa actacctata gacaggatga caagtacaaa
    14581 aagcaagaaa tagaacgtcc aatatatttt tttcaccgtt gttccctcct tacttgtatc
    14641 agtaaaaacg gaacgccaat caatgcaatg actaatccaa aaggtgtttc aaaaggggga
    14701 ttgatcatcc tcgctgtaaa atccgccaac ataaccaatc caccgccaac taaaaaagag
    14761 acaggtagaa cataacgata gtccgttcca accattccgc gaacaactgg cggaaccaac
    14821 agcccaataa atgaaacagg tccgactaac gaaacggcaa tggctgctaa cactgcaact
    14881 agacagaaaa caatcaaccg cgttcgttta ggatgacttc caagagctaa tgcatgtgtt
    14941 tcactcaaat gaatcaaaga tattgctttt cctaatgaaa aagaaaccaa aacagcaagt
    15001 atatataaag gaaacaatac tgctaattgt ttccatgtga tattggcagc tccgcctaca
    15061 aaccaaaaag caagatcctg ctgcagatta aagaattggg taagtgcttg gctgatagca
    15121 gtaaaaaaag aactaatagc tacgccagct aaaatcagct gtaaaggatt catgcccatt
    15181 ttcgaaaact gcaaaaaacc aaatacgagc aaagttacag caaaagaacc tgccaatgac
    15241 attagaaaca cagaaaaatc agaagcttta ggccaaaaag caaaactaag ggccattgcc
    15301 aacgaagcac ccgcgttgat tcctagtaag cctgaatctg ctaaaggatt tcttgtcacc
    15361 ccttgcatca atgcaccgct caatgcaaaa caactcccta caagaaatgc tcccgccaca
    15421 cgaggcaaac gaatatttcg tatgatttgg tgactttgtt ttgatctatc aaaatcaaac
    15481 aacgcctgcc aaaaatctgc tttttccaca gtcgctgcgc catttgaaag agcaacaaaa
    15541 aaaataatca aaagaacaaa acagaccaaa agcaggagtc tatttttctt catagtcctc
    15601 ataattgatc ctcctgctct tttggcaaca aatcataggt tagaaaaact ggtttatttg
    15661 ttcttggaag cgtgacaatc gttgcatcaa tctcaaatat ttttttcata cactcttttg
    15721 tgatcacttc ttcaggtgta ccttctgtga tcaacttacc gtttttcatt gctaataaat
    15781 aatctgaaaa acgtgaagca tgattaatgt catgaatcga catcaagatc gttgtttgtt
    15841 tcatttgatt gatttcagtc aataactgta ggatctctaa ttggtgtgcc ggatctaaaa
    15901 atgttgtcgg ttcatccaag atcaagatat ctgtttcctg agcaagcgcc atagcgatcc
    15961 agacacgctg ggtttgtcca ccagacagcg ttgcgatatc ccgcatacga aatccagcta
    16021 gacctgtagc atccaacgcc cactcaattg cttcatgatc ttgcgtgtcc atgccactga
    16081 acagtttttg ataagggtat cgtccgtaag atactacttc ttctactgtc atacctgtag
    16141 ggtgttcact agtttgtgct aataatgcaa ttttttttgc aagttctttt gaactgtatg
    16201 tagagatatc agagttattg acaatgatct ttcctgattt cacaggtagt atacgggtaa
    16261 aacttttcaa taaagtagat tttccacttc catttggccc aatcaaacta gtgattttcc
    16321 cttcaggaat cgtaacagac atgttttcaa tgattttttt gttttcgtag ccggctgcaa
    16381 tgtcagctgc tcttaatctc gtcatttcac ccatcctttc gctgaaaatc attctcaatt
    16441 atgacgctgt ttcaacattt cgtcaagcgc tactttcata tctatagcca aatgaagcgg
    16501 ttgacagcgt tcgaaatatc aggaataata accaatgaga aagattctca attatgttag
    16561 gaggaaagaa atgaaattaa aaacaaaagt attcacatta tctagcatat tgctgctggc
    16621 tggagtactg agcgcttgct ccccctcttc agataaagaa acagctacaa ctgccaattc
    16681 gacgacttca tccaatagta ctcatacagt cacagataca cttggacata aagtagagat
    16741 tccaaacgcg cccaaacgga tcatcggcag ttacttagaa gactatctga ttgcactagg
    16801 ggaaaaacct atcgcccagt ggactgttgg cagtggttcc attcaagatt atctgcaaga
    16861 tgatttaaag gatgtcccaa cgatttctta tgaccttcct tatgagaaag tgttaagctt
    16921 tgagcctgat ttgctgttga tttcttcttc cgctactgtc gaaggcggga aatacgaaca
    16981 atatagcaaa atcgcgccaa cttatgttgt aaaaaacgga acagatgtca catgggaaga
    17041 acaactaaaa gatgtcggca aagctttggc aaaagaaaaa gaggcagaaa aaatcatttc
    17101 cgattatcaa aaatccatca agaaaactcg agaagaacta gcagataaaa tcgagaacaa
    17161 gacagctgca gtgttgtggg tgaccaacaa ctcagccttt atggtcagca aaaatcggtc
    17221 aagtggaaga atcgtatacg acgatctgga atttggtgtt cctgatctag tggaagaggt
    17281 ctccaaaaaa gccacttctg actggtctgc tgtttcctct gaaaagctag ctgaattaga
    17341 tgctgactac atcatccttg tgaacagtga caaagatgct gccatgttca atgaaccaag
    17401 ctggaagaat ctaaaagcag tgacagacaa tcatgtactg caattcggac ctgaatctag
    17461 ttggctatat aacggaccta ttgccagtca ggaaatggtc aatgatatca aagaaaaact
    17521 aaattgattt taactaaaaa aagcatcttc acgattagtc gtgagatgct ttttttctta
    17581 ttaacaaact agacgcatag attttattct tcttttgaag caaccctatt ttttactctt
    17641 tcccattttt gaaaacgatg ggcatataga ttttcttgat ccgttatccc ctcatactcc
    17701 tctataaggg cgaagtctga aaaatcaata ttcccaatat acgtatcgcc ttcaaacgtg
    17761 ctatcgatca atgttcgcca gatccgacca gtgtaaggaa gaagttcttg aaaaatccga
    17821 ctaccacccg aaacatagat atcttctgag atcgtttgac tatacgacaa aagttcatcg
    17881 atcgaatgca ttacttccgc attttctttc gtgcgaaaat ttggttgcgt tgtcaacacg
    17941 atgatatggc gataaggaag tgaaagatcc cccattcctt cataggtctt tcttcccatc
    18001 actaaaattc gatccattgt atgttcgcga aagaaacgca tatcatttgg caatcgccat
    18061 ggtaacaaac catttttgcc gatcagacca tttttatctt gtgcccacat ggaaataaac
    18121 atattatccg ctccttatga ttttagatgt tttgagataa aaaaagtctc aattctttga
    18181 ccagcaagga attgagaact ttaaaaacat tattttgttt cacggtgcaa agtaactttg
    18241 cgttcgcgtg ggcaatattt ttgtttttcc aaacgatcag gattgttacg tttgtttttg
    18301 cttgttagat agttacgttc tttacaagaa gtacactcta aagtgatgtt tacgcgcatg
    18361 tttttccctc ccatttttac aagaatcctc taagatgact ctcagacttg attatcttac
    18421 catgattttt tctagattgc tagtgttttt cacgattttg ttaagtaaaa ctacttgacg
    18481 ataaatacaa cggttttact tctcaaacaa aatctttttc tttttgtata aataccctta
    18541 cacttgttta tatagattat gttttttaat aaagggggat actaatatgc catggaacat
    18601 gaaggacttt cctgcatcaa tgaaaaactt agataaattg acaagaaaaa aagcaatcga
    18661 catcgcaaat gcattattag atgaagggta ccctgataac cgtgcgatcc caattgccat
    18721 tgatcaagca aaaaaatggt atgaagatgc tagtgaatca gaaaaacgaa ctttcgaaaa
    18781 agaaaaaaat ccaagcaaga cggatgaaca cgatacgaat cctcgtgctg gaaaactgct
    18841 tgattcagat gtgatcgtag aatatgagga agaacaatgg atcgtgaaat ctaaaggggc
    18901 aaaaaaggca agcaaccatt ttgatacaaa aaaagaagca atcgaaaaag gaaagcaagt
    18961 agcacaaaac aaagaatcaa ctttgttcat ttataaaaaa gatgggacaa aagaaaaaga
    19021 aatcgattat tgaacatatg taaaggggtt gtgacgaaaa tcgtgtcaca acccccttat
    19081 gttcgaaaca atggtgttcc tctgccaact ccgaaccata agtcataata tctataaaac
    19141 gggaagagct gttttttgaa attgtttctt atcactcagt gttaaacggt aggagaaaac
    19201 ctctttttta ttgatatttg attttcatca aacagtcaca tagtcgattg cttctgcttg
    19261 cggaatcgtt tcatctacaa agtagacttt ataccatgtc aagaaactga agattgtcca
    19321 gatttttctt tgctcttctt tacgtccttc gtgatggtca tctagcaact tcatgatttt
    19381 ttcttgatca aaaaattctt ttgcaaaatc tgcttcgaat aatgctttga cttgttcgta
    19441 gccgtgttct tctttcaacc aagctttgat tggcacaggg aatcctaatt tcacacgatt
    19501 cgaccattct tccggaagat gtttgttcgc agcttgtctg aaaatatctt ttgtgttgtt
    19561 ttgattcaat aaaaacttac ttggtatttg ttgagccaat ttcatgactt cgatatccaa
    19621 taaaggtact cggacttcca aagaacttgc catagaaagt ttatctgcct ttaacaagat
    19681 atcttttggc atccattgat gaagatcgac atattgcatc ttattgactt catctttgat
    19741 tccttctgtt ttctcgtaat gaacagtcat gatttcatcc acagatggtg ctgtttgata
    19801 ttttggtgtt aacacttctg atgcttctga ttcttcgaac actcgagcat gaccgataaa
    19861 atattctttt gcaggagcaa gtgattcata taaatggatc cgtccatgga agtttttcat
    19921 ttttccgatt ttacgtccta aattatagcg tgttcctttt ggtaattttt tcaatccttg
    19981 agcaatcaca cggatgaatt ttgaattcgt atggaatcca tacgtttggt atcccgcaaa
    20041 cagttcatcc gccccttctc cagattggac tacccgcaca ctttgtgaag caagatttgc
    20101 taaaaagtac aaaggtacgc aagaaggatt tgaatcaggt tcatctaagt gatactggat
    20161 caaagggaat gctttgaacg acatgtctcc gtcgatcact gctgctgtat tatctaagtc
    20221 caatagttcg gttagttttc tggcttcaat cgcttcatta taggttttat catcaaaccc
    20281 gattgagaaa gaatgatctg gtttcaatac agaggttaca taactggaat caaccccgct
    20341 ggataagaaa gaaccaactt ctacatcact gaccgtatgt gcttcaatag aagcttgaac
    20401 ggtttcgtcg attttttcga tccattcttg tcgggaatgc gtttcttttg tttcaaaatc
    20461 tgcatcccaa tattgctgaa tgtctatttt tccatcttta tacgtataat aatggccttc
    20521 aggcaaacga tagacatctt tgaagaatgt ttcgccgttt aatggagaat attggaaagt
    20581 catataaggt ttcaacgctt ctttgttcaa ttctttgtta aaatcaggat gaggcagaaa
    20641 acttttgatt tctgaaccaa acataaatgt tccgttcatt tctgcataat aatatggttt
    20701 gatcccaaag tggtctcttg caccgaataa ctcattcttt tcattatccc aaatagcaaa
    20761 agcaaacatt ccgcggatct tttgcaataa ttctgttccc cattcttcgt aaccatgcaa
    20821 aaggacttct gtatctgcat gtgtttgaaa aacgtggcca gctgcaatca attcttctct
    20881 caaaggctgg taattataga tttcgccatt aaaaataatg atctttgtcc gatcttcatt
    20941 gtaaataggc tgagtaccgc cctctaagtc aataatactt aatcttctga atcctaaagc
    21001 aacatgttta tcgatatatt ctcctgaact attaggtcca cggtgaacga ttcgatccat
    21061 cattgtatta ataattgttt ttttgttgtc cttgtcgtta acaaaaccta caataccgca
    21121 catttaactt tcacatccta atataaaatt actgtttatt tcattcatat gcatcttatc
    21181 atagaataaa tagcggacga atattttctc tcttacaatt tcgtcacgtg aaaacaaaag
    21241 atgaaagttt ctgtttttac agaaaataaa gtcccaatat ctaatttacc atgttttctt
    21301 ttttgaggct accttattct cttcgtgtta aaaaaatatt agtggatgct atttccaaaa
    21361 aaaccagcaa aaaaagacac gacctaaaga tcgtgt
//
LOCUS       DARBEC010000049        21343 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.049, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000049 DARBEC010000000
VERSION     DARBEC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21343
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.049"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..152
                     /locus_tag="SCL80_001672"
     CDS             <1..152
                     /locus_tag="SCL80_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1107542.1"
                     /translation="NFAKNQGYNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIY
                     MKKTL"
     gene            complement(230..763)
                     /locus_tag="SCL80_001673"
     CDS             complement(230..763)
                     /locus_tag="SCL80_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEG1107543.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCL80_001674"
     CDS             complement(857..2035)
                     /gene="mutY"
                     /locus_tag="SCL80_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEG1107544.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCL80_001675"
     CDS             complement(2028..2825)
                     /gene="recX"
                     /locus_tag="SCL80_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEG1107545.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2932..4314
                     /gene="rlmD"
                     /locus_tag="SCL80_001676"
     CDS             2932..4314
                     /gene="rlmD"
                     /locus_tag="SCL80_001676"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEG1107546.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4488..5066)
                     /locus_tag="SCL80_001677"
     CDS             complement(4488..5066)
                     /locus_tag="SCL80_001677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEG1107547.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5388..5858
                     /locus_tag="SCL80_001678"
     CDS             5388..5858
                     /locus_tag="SCL80_001678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEG1107548.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLTMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5966..7192)
                     /locus_tag="SCL80_001679"
     CDS             complement(5966..7192)
                     /locus_tag="SCL80_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1107549.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7946..8146)
                     /locus_tag="SCL80_001680"
     CDS             complement(7946..8146)
                     /locus_tag="SCL80_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1107550.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8280..8888)
                     /locus_tag="SCL80_001681"
     CDS             complement(8280..8888)
                     /locus_tag="SCL80_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEG1107551.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8973..9443)
                     /locus_tag="SCL80_001682"
     CDS             complement(8973..9443)
                     /locus_tag="SCL80_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEG1107552.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9491..10405)
                     /locus_tag="SCL80_001683"
     CDS             complement(9491..10405)
                     /locus_tag="SCL80_001683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEG1107553.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10551..11354
                     /locus_tag="SCL80_001684"
     CDS             10551..11354
                     /locus_tag="SCL80_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEG1107554.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11532..12479
                     /locus_tag="SCL80_001685"
     CDS             11532..12479
                     /locus_tag="SCL80_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEG1107555.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12547..12840)
                     /locus_tag="SCL80_001686"
     CDS             complement(12547..12840)
                     /locus_tag="SCL80_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEG1107556.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEMDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12868..13533)
                     /locus_tag="SCL80_001687"
     CDS             complement(12868..13533)
                     /locus_tag="SCL80_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEG1107557.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13673..14305)
                     /locus_tag="SCL80_001688"
     CDS             complement(13673..14305)
                     /locus_tag="SCL80_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107558.1"
                     /translation="MIKVLLVDDHEMVRLGVSSYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14312..15379)
                     /locus_tag="SCL80_001689"
     CDS             complement(14312..15379)
                     /locus_tag="SCL80_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1107559.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLLAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15376..16107)
                     /locus_tag="SCL80_001690"
     CDS             complement(15376..16107)
                     /locus_tag="SCL80_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEG1107560.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCL80_001691"
     CDS             complement(16276..16755)
                     /gene="greA"
                     /locus_tag="SCL80_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEG1107561.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCL80_001692"
     CDS             complement(16891..18066)
                     /gene="mltG"
                     /locus_tag="SCL80_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEG1107562.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18270..19439
                     /locus_tag="SCL80_001693"
     CDS             18270..19439
                     /locus_tag="SCL80_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEG1107563.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19706..21340)
                     /locus_tag="SCL80_001694"
     CDS             complement(19706..21340)
                     /locus_tag="SCL80_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107564.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6585 a         4179 c         3366 g         7213 t
ORIGIN      
        1 aaaactttgc taagaatcaa ggctataata cgattttatt aggggcacag tccacagctg
       61 aaacttttta cgaaaaacta ggatatacag cttacggcga tccttttgaa gatgctggca
      121 tgcctcatat ttatatgaaa aagactttat aaaaatttat ttcgaaaaaa gcaaaaaaca
      181 gcaagctttt gagaaaaatc tctcactggc ttactgttct tttttgtgtt tacttacgcg
      241 acaattgctt gtaacggtta taccaaatat caatatagcc ttccgagaac gggccatctc
      301 cattattgat ccagtcaacc agaatcttca cattttcttt taggatgaag tcaatatcgt
      361 tcgggtaatt catcattttg ctgtgcatct catattcatc gacatccaat agtcgtttct
      421 cgccgtcagg aaataccttg atatccaagt catagtcgat atatttcaag gcctcatcgt
      481 ccagcaaaaa aggtgaagct agattacaat aataggaaac ccccttttct ctgatcattg
      541 ctattacatt gaaccagtat tttttatgaa aataaacaat cgctggttcg cgcgttaccc
      601 atcggcgtcc atcagattca gtgactaatg tgtgatcatt cacgccgatc atagagtgtt
      661 cgcttgtttt taataccatg gtatctcgcc acgttcgatg caatttgccg tcgtgcttgt
      721 aactttggat cgtcacaaac tctccttctt ttggaactcc catagcaacc cttccttaag
      781 cagcattttg tgctttgtgt ttgggtgttc attacaccga catcataatt attatatcaa
      841 taaaaatgag ctttgcctag aaaggattcc tattgtttcg atttttctct aactgatcta
      901 ccagtttcat ttggacctta ggaaaaacga ccgaatcaaa tgcatcaggc ttcaaccact
      961 tcgaagtttt gttctcggta aattcttgtt ctgctccctc ggttgcccgt ccgtaaaaaa
     1021 ggagaacatg ccatttcaga tgactaaaaa tatgagtaac ttctcctaaa tgtcgtgtct
     1081 gccaaacaaa gagttcctgc ttttcaaatg ggagattttg ctcttcttct gctactaggt
     1141 catcaaacag atcgatttct ggctttgttt cccattcttt tttcaatcgc tcatactcct
     1201 cttgtgtcac ttccatcatt ggaaatgtcc acatattcgc cagcaatttt tggctatctc
     1261 tttcttcaaa ataatacgct ccagaatgat tttgaagagc tgcactgata taataaacgt
     1321 cttttggctt tgcttttttt gtttttacag gaaaactcgt ttggattcct cgtttgttag
     1381 ctaaacaaaa ggcttggatc ggacaagtct cacactttgg agaagttggt gtacagatag
     1441 ctgaacctag atccatcatt gcttggttga attcacctgg atgcttttca tcaatgattt
     1501 tccgcatcgc ttcatcaaag attttcctgc tagaagcttt tgcgatgtct gcttcaatgc
     1561 aaaacaatct gctgactact cgcatcacgt tgccgtcaac tgcaggttct ggaagtccaa
     1621 atgcgatact tgcaatcgcg cctgtcgtat atggtcctat tcccttcaat gagctaattt
     1681 cttcaggcgt ttgcggcatt tccccatcaa attctgacat gatctgttta gcagcagctt
     1741 ggatgttccg ggctcttgag taataaccaa ggccctccca tgcttttaaa agcttttctt
     1801 ctggagcatt tgctagctct tcaattgtag gaaaccattc catgaaacga taaaaataat
     1861 cgatcactgt gtctactctg gtttgctgaa gcatgatctc agagatccag atacgataag
     1921 gatcacgatt atagcgccaa ggcaggtttc ttttttcttg ttcataccat tgaatgaact
     1981 gatcttgaaa ttcttttgtt tctttatctg tccatttttc ccattcttta ttcactttcc
     2041 tcttcctttt ccgtaatgaa tcgctggatc atatccaaat caaatccttt ttgatataag
     2101 ctttgcttta cttttagatt tcttttttta ggttcaaacc ttaaatttct tcgccatagt
     2161 ttatctcctt gtttgactaa ataatcatat tctatttctt gatctgcttc ttgcggaaga
     2221 tcagctatcg cttgttggat cacttcttga gaaaagcctt tcgtcatcaa tgtttgttgg
     2281 atttttctca ataattcttt ttggcttttt ccgtgatttt ttccaaacgc tttttcggct
     2341 gtctgatgtg cattttgaac ttggtcttca aatgtatatt ggctgagcgc ttgttcaatg
     2401 atttcaggtt tcaaaccttt ttgctgcatc tgctgcgcta atttctttgg tcctttgtca
     2461 cttaagcgca tattcgtgcg cacataactt tccccataga ccaaatcatc gacaaggtcc
     2521 aattctttta agcgggctac gattttattt cgatcttctt gaaggatttc cttttctttt
     2581 aagtatgttc ggatttcttt ttccgatcgc aactgataac ttaagtaatt cattgctagg
     2641 tgaaaaccga aatcagctcc gctactcttc ttgatctctt ggatagtttc ttcgtctaat
     2701 tcccgtcctt ttaataatcg atagcgtact aatacatctt cagatacttg taaagcttct
     2761 ccttcagata aatctacgcg atagaaaggg cctttgcctt tactgattct cgtaacggtt
     2821 aacataaaca tcctcctcga acatatattc tctatcatac cacagaaaat ccccgtaatc
     2881 aggtattata tgttagaata aaccacagaa aaaagaaaaa gggagatact tatggcagaa
     2941 acaattgtca aaactggaca aacggttcct ttgaaaatca agcgtttagg cataaatggt
     3001 gaaggaatcg gttatttcaa acggttgatc atatttgttc catatgcact accaaaagaa
     3061 gaagtactag tgaaaatcac gaaagcaact cctcgctatg cagaaggaga attaataaaa
     3121 gtcaaaaaaa caagcaaaga tcgcgtagtg gcaccatgtc ctgtttacta tgaatgcggt
     3181 ggctgtcaat tacaacactt agcttatcaa gctcaattgg attttaaaaa ggatctgctt
     3241 ttgcaagcat tggaaaaatt caaaccagca ggctttcgat cttatcaact tttgccgacg
     3301 atcgggatgg atgagccttg gcattataga aacaaagcac aatttcagtt acgaaaaaat
     3361 aagcagactc aaaaaattga agctggttta tacgaagcaa actctcatca gttagtccct
     3421 ataacagact gcttggttca agaacctcga acacaaaaag taatgaatac tgttgttcaa
     3481 ctattgaata aatttgatgc acccatctat gatgaacgga caaacagtgg aatcttccgc
     3541 acaatcatgg ttagaatcgg tgttaaaact ggggagttac aagttgtttt tattactcgc
     3601 agtaaaaaat tcccgcaaaa aaacgcaatg atccgtgaga tcaatgaacg actgcccgaa
     3661 gttgtttcaa tcatgcaaaa tgtccaggca caaaaaacgt ctctcgtgat gggggatgaa
     3721 acgatccact tatggggaaa agaagccatt gaagaacaaa tcaatgaagt gactttcgat
     3781 ctttctcctc gtgccttctt tcaactcaat cctcagcaga ccgaattgct ttatggcgaa
     3841 ggaatcaaag cattagatgt ccagcctaat gaaacgatcg tggatgctta ttgcggagtt
     3901 gggacgatcg gcttaagtgt agcaaaacaa gccaaagaag tacggggaat ggataccgtt
     3961 cctcaagcaa tcgctgatgc aaaaaaaaat gcggaaagat taggatatca caacactcga
     4021 tatgaagtcg ggacagcaga gcaagttctg cctaaatggt tgaatgaagg ttttcagcct
     4081 gatggtatca tcgtagatcc accacgtacg ggtttggata acgcattgat ccaatcatta
     4141 ttgaaatacc ctccaaaaaa acttgtttat atctcctgta atgtttctac acttgccaga
     4201 gatctggtta atttaagtaa aaagttcaag attgaatatt tacagtccgt cgacatgttt
     4261 cctcagactg cacgttgtga agttgtcgtg aagatgacga gactcgctga ctaaatgaaa
     4321 gaggttgtga aaaagtctgc cctgtatcaa gtaataagaa cagccaaaca aaaatagctc
     4381 ttcatgtttt atgaatactg tacttgtgac cgaagagtta acaggaggac actatttatt
     4441 taaaaaaagg gttgtgacat agtttttgtc acagcccttt ttgattatta ttggatattc
     4501 acgtctatca cttgataaaa tgcatttgtc gtatcgctga tattccatac tgctaaaatc
     4561 acattatatc cttttcgatc ctgcggaagt gtgatcgttt ggttgactgt ttttggtgga
     4621 acagttgctt tgtcgtctat ttttgtgatc aattcaaaat cactaaattt caaaggttga
     4681 ttaggattcc aagttggttt ggtaatgtag taatcccaag tacttgtccg atgttgtgct
     4741 gttaaattcc aagtaatatt taatgttcct ggattaacca tgtttttatg ccatctgtta
     4801 gatgtctgtt catctaaagg ctcaaagccg ctgactcctg cactagcaat ttttccatca
     4861 atgaatgtat ttttggtagc ttcgatactt tgcggttccc attgtgctct tccgacattt
     4921 tgatttaagt ttcctgctgc tgatgttcct aggtatgcac ggcttcctgg agaagtaata
     4981 taaccatgcg caaaacctgg aatgctaaaa ccgaacaacc ctaaactaac taacccaata
     5041 aacaataacg tactaatctt tttcatgtag aaacctcctt catttgataa ccacattgta
     5101 aacgctttat tgtattaaca caaacattat gcttttataa ttttatgttg aagtaaaaaa
     5161 ggtgaaaaaa gtaagatgca gattataaca gaagtagacg agaaaagtac aaatagaaaa
     5221 catgagaatc aaatcatatg atctttttca aaaacatcat tcatataaac aaatgttcaa
     5281 ctttttaata acaacaatta tcaatttttt tcttacttca caaactaaat acaggtcttg
     5341 ctgtgagcct tcatggtaaa ctaatattat agaaaggagt tagtaaaatg aagatgataa
     5401 aagatattcg tttttgggtt tgtgttatag gaattgtcat attaggcttt ttatcagggt
     5461 tgcttagcgg aaatcccgga aaatattact attctttgca acttccacca tttgcaccac
     5521 ctagctggat atttgggccg atgtggacct tactgtatat attgatgggt atttctcttt
     5581 atttgttact taatcacaac aacaaaaaac agcgaaacaa tcttgtcggt ctttttgtga
     5641 ttcagttcat ttttaatttt atatggtctg ctctattttt taatttaaga aatattttca
     5701 ttgcagcaat cgatattaca cttttagtta ttttcttgtc agtgcttatg tatcagttgt
     5761 ggctgcatca tcgcctgaca atgtggctca tgattcctta ttatttatgg gtactattcg
     5821 caactctatt gaattattca atttattttt taaactaagc gctaaaaaga gacgtcctgt
     5881 aatttaacac atagaaaaag tgccacctaa gttagatttt ttgtctaatt taggtggcac
     5941 tctttattaa ccaaactatt tttttctagt acccacttgt tgttccatac tcattattct
     6001 ggtcataacc ataaccattt tgttcctgtc cagcgtaagg atcataactt tgagtatctt
     6061 gacttggtgt atagtactga cttgtatctt ctgtttgctg ataataggta ttcggatctt
     6121 ggtactgatc atatgtgttt tctgaatagg aagaataatc ggtgtaccct tcactattcg
     6181 atccatcacc gtttgtcgaa tcgccatctt ccgaatcatc aaagaacatc attgaagcat
     6241 tctcattttt caaattaggt aatgtatcgc ttgtttttat atttaattgt ttcttcagtt
     6301 cattttggat cgaaagaaga tcattttttc ctaaaatttg atagtagata tcattgatca
     6361 tttgtccttc gccttgcaac tggtcttgtt tgattgtttc aaacgccggg gtgtagttcg
     6421 tcgctacatc aagcatgtca tcccaatcta agtccgtcgt cacattgttc tcaactgctt
     6481 ttaaaatttt tctataattg cttacgccat ctaaacttaa aactttattg acgattttcg
     6541 tcactacttc ccgctgacga cgttgtcgac cgatatcgcc ttctggatct tcgtgtcgca
     6601 ttcgagaata ggctaaacct ttttctccat tcaatgtttg ttcacctact ggtacatgga
     6661 caccatccaa agtaaagtca attttattat tcactttgat gccaccgacc gcattgatca
     6721 aatcttccat gccatccaaa ttgatcgtta cataatgatc aataggaata tcgagcagtt
     6781 gctcaattga atccatggcc atttcgacac caccaaatgc gtaggcatga tttaatttat
     6841 ctaccgttcc ataacctacg atattcgtat aaatatctcg gtccaaactg atgatcgttg
     6901 attttttttg tttcggatta actgttacta ccatcatcgt atcagaacgt ccttgttccg
     6961 ttcttcctaa tccaccggta tctaaaccta atagcaaaac cgaaaaaggt tctttgtcat
     7021 caatgcttac tctctcagaa cgtttggagg atattcgatt agagttttta gaaattttgt
     7081 ttactgtttg gctcgcttct cccataaatc tgattccgta tgctgataat ccggcaatca
     7141 gcaaaacgaa tacccctaac aaggttaata gaactttttg ccatcgcttc ataatacacc
     7201 tctgtttcac attttctaaa atattgtctc atataactgt tcaatcagca aattcacttt
     7261 ttaatcataa gaaaacttta aaaaacattt ttgtgtaacg attattattt tttaaaattt
     7321 ctcaaaattc ttaactatca taccataccg tgaaaaagat cgaaagaccg aatacacgca
     7381 gttcgcaatc gttttatctt tttttatgta ttttttccag aaaaagcgaa caacaaggta
     7441 ttttccaaat aatcgtgcag taaaatgatc gcacatacct ttcccatctt tttaacagtc
     7501 tttgcctacc gttcagatta tcattcgatt tcacgacaat ttcttcctat gactggtcct
     7561 tttgttaaac ccatcttcta ttcgttttat ttccagctca ctgcatggct cttaaaatga
     7621 ctcgaactag tgattgcatt accatcatct tttaatggat agatatgctc ctcttcaaag
     7681 taaaacgatt ttcttttaaa gtaatagaat tgacatggta atgagtatgt atactagtgg
     7741 tttcacaaat ggtcttttat tcaacatcca aaaactgaaa tttctttaaa atccattact
     7801 aacgaagaaa atgttccttg cttgataatt agttaggaca tgtcaagtga acaaatgcat
     7861 ttacttagcc aaaaaatcta aaaacttcat aaatagacaa aaagacacga ctctctaaga
     7921 agtcgtgtct ttattcaaca aattattata atttagtcac ttcagcagct tgaggtccgc
     7981 gtgcaccttc aacaatcgtg aattctactt cttgaccttc ttctaggctc ttgaagccgt
     8041 ctccttgaat tgctgagaaa tgaacaaata cgtcatctcc gccgtctact gtaataaacc
     8101 caaatccttt ttcattgttg aaccatttaa ccgttccgtg ttccataaat gtatatcctc
     8161 caaaaaagtt cttgcaagta accttgcaag aacattatac ccaatttaga aaacgctagc
     8221 aagctttcgc tcttttattt tccaaattat ttttaagact gatatgacgg aggaaatggt
     8281 tattttgctg cttcgaaacg tttgtttact tcatcccaat taataacatt ccagaatgct
     8341 gcaatataat ctggtcggac atttttatat ttcaaataat aagcatgttc ccaaacgtcc
     8401 aaacctagaa ttggtgtctt tccttccatc aatggagaat cttgatttgc agtagaggta
     8461 atagctaatt ttccattttc cattacaagc caagcccatc cagaaccaaa tcgtccagcg
     8521 gctgcttttt tgaattcttc tttaaaagaa gaaaaatcac caaaagcttc attaatcgct
     8581 tcttttattt ctcctgtagg ttcgccacca gcatttggtg ccatgatttc ccagaaaaat
     8641 gaatggttag catgtccgcc accattgtta cgtacagctg tcttgatatc tgttggaata
     8701 gcgtccatat cagataataa ttcttctatt gttttttcgc ctaattctgg gtatttctca
     8761 attgctgaat ttaaattcgt cacataagta ttgtgatgct tatcatgatg cagatgcatc
     8821 gtttcttcgt caatataagg ttccaatgcg tcatatgcat aaggtagatc tggtaatgtg
     8881 taagtcataa aaaaattcct cctcaatgtt ttcttaataa tgtctacatt acaaagagta
     8941 acaaagagaa ggaaattctt caattgttat gcctagtcgt ctttttcact ttgacctact
     9001 tcttcttcaa aaacctcagt ttctttaaaa ataaacaact tgctgaaaat atagttcgca
     9061 aggataacaa taatttgagt gatgattttc gccacatact cattgctgtt cataacattg
     9121 atcaacaata ccattgcccc catgtccaat ccgtaagaaa ggatacggta gaagaagaac
     9181 ttaccgcatt caataagtgc atgtgtaaaa ttcggtgatt ttgaatggaa tacccaaagt
     9241 ttgttcgtta caaaagcaaa caatacagaa caaaaccacg aaatagaatt ggataaggcc
     9301 atacttaaat caaaatattg gcgtgcgaga gcaaaactaa caaaatttac aatcgtagcc
     9361 aaaccaccaa aaaagagata gactagtatc tcccaatatc cttttgcttc taaatatcct
     9421 tttaactgtc gatactgctt catgagccaa cctaccttat taaaaattct tatttatcat
     9481 actctatatt tcaataactt tcatctttat ttaaaaaaat taattttttt tgatcctttg
     9541 cctttgtctc tgcttggatc gtcacatgtg taataccata ttttaatttt aataaagaaa
     9601 ttatacgagg atatatcttt tcaatctcgc ttagtagctg atcttcaaaa tcaatatgtc
     9661 cgtcgaaaat aattatcttt tcgctatact gccaaatatg gacatgatga acatccttta
     9721 ccccatcgat tgcttcaatt tcttgttcca ttttttcata gtcgatagca ggtgcacttt
     9781 gtatcaaaat agcggcagct tttttgatca ccattatcgc ttctatcaat atatatatcg
     9841 aaatgatcag tgtaataact ggatcgatcc agtagatatc gaaaaaacgg atcaaaattg
     9901 ctccgataat cacacttacc gaagacagtg tatcacttaa caaatgcaaa taactagatt
     9961 ttatattcaa actttcatct gcttcttgtc gtaataataa tactgagaac aaattcgcga
    10021 gcaatccaat aactgctact gttagcatca aatgactatt tatcttttct ggtgaagtga
    10081 accgcttgaa tgcttcaacg gttaaaaaca aggataatat gatcaatatg aatgagttga
    10141 tgaaagcact caatatttct aaacgctggt agccatacgt acgtttttta tttgcttctt
    10201 ttcgggccat tttttgtgcc ccgtaagaaa aaataattgc caatgtatca ctaaggttat
    10261 ggaaagcatc tgaaacaaga gacaaactcc ctgacataat ccctccaata aattctgata
    10321 aagtgatcac cgcattcaaa accactactc caaatagttt ttttgatgat ctctgtggct
    10381 gtgtaggcac tattccttcc tccattttat ttatattgga tagtctttag acaagaataa
    10441 catttttttg atatttcacc aatcattcag ctgtttcctt aaagcacttg tatttcactt
    10501 gatacttcgc tagaattgca agtgatgaca ctactagatg aggagtactt atgacaacaa
    10561 aacaattaat tatcagttcc tttatacgat tcaaagacct aaaggaggcg agaaatgtcc
    10621 cattttggaa agccattgtc tatcttttag ttttgagtat aattatggct ttgccaatct
    10681 cttttcaagt ttttcaagtc ttaaacaaca ttcaattgga tgggcaaaag attgctactc
    10741 gcattcctga ttttacaatc caaaacgggc agcttcaaac cgaagaaaaa gaaggattca
    10801 tttaccaaac aaactccatt atcttcacat ttgatccaga gggaaaacga acagaacagg
    10861 acatctctac tgatttgatg gggaattttg ttagtgtcgg gatgttaaaa aacaaactta
    10921 tcatagctct tcctaataca ggcacgactt ctgctttgtt gaacaataac caattagaac
    10981 ttccctacac caatgaatct ttaaaaaact taacaggtaa gcagctccgt tcttttttaa
    11041 gtgaagcatc tataccactt tggataaaag tcctgacatt tatattttct atttatccta
    11101 gttttctgaa tctgctgatc actttgctat ttaccaacat tgctgctttt ctctatgcac
    11161 gctttcgact aacaaaagct acttttcttg attgtttaaa aacattgatc tattcaattt
    11221 cattgcctac tataattgca acaatcttga tggtctttct tccttctttt gacactagcg
    11281 ccttcattgc ttttgcagga atctttattt ttgctcaagc tgtcaaagga tgggcaaaaa
    11341 tatcgatcaa ttaagaaata aagacaactc gttatttcta cttcaataga cccttttaga
    11401 ggttgtgaca tttttttgtc acagcccctt ttttatttga aaaatatttc tttctcaaaa
    11461 aacaagcaag gcgcttcaaa gttcttcttg tatgcgctac acttagagta aagaaaggcg
    11521 ggaaattaac tatgtcagaa agaatcattt atgcagatcg tccatttcgt gatgaattta
    11581 tcgaagaaat ccatactata gcgcctggtt atatctttaa aacagaatta caaccagagg
    11641 atctacaaaa tgtagagatc agtttgggct ggaactcaga ctaccaagaa aaactattag
    11701 cctcttctac cttgaaatgg gtccaatcta tctcagctgg tgttgatcag ctaccccttg
    11761 agactttttc aaagaaacat attttacttt caaatggtag tggcatccac agtgaatcga
    11821 ttacggagca tgtcttagga atcatccttg ggtattctcg tggtcttttt caagcacaaa
    11881 aggcacagag tgacatgaaa tggttaggtt catcggtcca ttaccaatct atccaaggga
    11941 aaaaaatact aatcattgga actggccata ttggaaaaat gattgcaaaa aaatgcaaag
    12001 cattagacat gaactgctat ggaatcaata catccggtca tccagcagaa ggaatggaaa
    12061 agactttttc tttaaatcaa ctaaaggaag ttcttcctga agtagatttc gtagtcaata
    12121 tcttacctct aacggaggaa acaactgggc tctttgatca gcatatgttt gaacactttg
    12181 atccaaaatc tgtattcatc aatgttggcc gtggtgcttc tgttaagaca caagatctgg
    12241 tgcaggcttt aaataaccag caactttcct ttgcagcatt agacgttttt gaggaagagc
    12301 ctcttccaca agatcatcct ctttggaaaa tggacaatgt actgattact tcccacattg
    12361 caggtttgac acctgatttt caaaagaaat taatggctat ttttttaacc aatctgaaaa
    12421 gctactttgc cacccatgag cttcaaacca atcaagtaaa attgtctgca ggatattaat
    12481 agctgaaaac aatattttca aaagaaaaaa gagaaggtga caaaaattgt gtcaccctct
    12541 ctttttttat tgttttttaa agtgataggt tggctcctcc aggttgggat cataatcaac
    12601 aagtaaatca tatcctttaa agaaccattc atctgcttcc tcgataaaaa aaagtgtccc
    12661 atccatttcc actttcttta caggatgttc tggctgttca actgacattc caatcgaaaa
    12721 accatcatga acctctgttt tgccgtagac ttttccaaaa aaacgtattc cgtaagtttc
    12781 tggcagatca atttctgagt caaaccactt ttgtgctttt ggcgttaccg ttaatttcat
    12841 cccaacaacc tactttctct tttctttcta ttgattcatc ttatcgtcta gaatatccac
    12901 atcgcctgtc tctccaataa ccagtagatt atcattttca cgaacatatt catctgctgc
    12961 aggagaagcg ataaccgttt gatctactct tcgtatggcg actactgtta agtcaaaacg
    13021 ttgcctgaaa tttaattcag ctaatgtttt gttgaaaaat ttaggattgg agactcggat
    13081 ttctgctaaa gaatatttat ctgacaactc taggtaatct aaaatattct ttgacactaa
    13141 attatgcgca atccttattc ccatgtctcg ctcgggatgg acgacgcgat cagctccgat
    13201 cttttcaagt actctggcat gatactcatt ttgggctttt gctaaaatat ttggtacgcc
    13261 catttccttt accatcagag taactaagat actggcttga atatcttctc caatagcgac
    13321 aattacatga tcgaaatttc ttatgcctaa tgatcgaagt gtcatttcat cctgtgcatt
    13381 tgccacaaca gcatgagtgg ctatattcat atattcattg acccgatctt cattactatc
    13441 aatagccagt acttcttgac cagcttcgat cagtgttcgg cagatacttc cgccaaatcg
    13501 tccaagacct acaattgcaa aattttgttt caccgttttc ctcctttagt atcttctttt
    13561 ctcagtataa caaagccttt acaaaaacaa aagcaagcat cattattgta aaaaagccaa
    13621 gaaaaataat agttattttc tcagcttttg attatcctct ttatcatcgt ttttatttca
    13681 ccaaaccatg tttgaatgca tagatagcgg cttgtgtacg gtcttccact tctagcttag
    13741 ccaatatatt ggaaacatgt gtttttactg ttttcagtgt aataaacaat tcgtccgcta
    13801 tttcctgatt actttttcct tcagaaatca gcattaaaat ttcattttcc cgattcgtta
    13861 gttcttcatg taatacaggc tcatttcgcc gactcatttt ttccatcatt tttgttgtca
    13921 cttctggttc taatactcgc tcgccacgtt gcgttgccct tatagcatca gcaatttcat
    13981 gtgcggtaga ggtcttcaac aaataccctg ctgcacctgc ttcaatggca ggataaactt
    14041 tttcgtcatc aataaaactg gttacgataa tgattttagc ttctggccaa tcttttaaga
    14101 ttgcttttgt tgattcgata ccatccattt cttccatgac taagtccatt aaaatgacgt
    14161 ccggtcgaag tgtcatcgcc ttctcatagc cttgtcgtcc attttcagct tctccaatta
    14221 cttcaatgtc ttcttgaata gataaataag aagagacgcc taagcgcacc atttcatggt
    14281 catctactaa taaaactttt atcatcctta tctatgcctc cttcatcaaa gggaccttga
    14341 tttctacact tgtaccttga cctttaaaac taatgatttt tactgttcca ccaataccag
    14401 ctactcgttc tttgatattg ttcaatccat aacttcccgt tttcgtttca ttcatattaa
    14461 accctgttcc gtcgtcaata atacgtaaca aaagattatt gtctattttg tgcaaatata
    14521 cctctaattc attggcttta gcatgtctta atgtatttga taacaattct tgaacgattc
    14581 ggaacaagtg atcctcaatg gaactagata gttttacatc ttctacatcc catttcagcg
    14641 aaatctggat tttattttgc aattccttca ataactgctc gataccttgc tttaaacttt
    14701 tctcttctaa attgactgga cgtagatgaa gaagcagcgc acgcatctca gactgggaag
    14761 cgttgatgat ctctgctacc attttcaact gcttgcgaaa catttctggt gtctcgcttt
    14821 tttctgcttg ctccgtcaat gccgacatca tcatcattgc tgcaaacaat tgttgcgaaa
    14881 ctgaatcatg cagctcacgg gctaatcgat gtctttctaa ttctaaaatt tcttctttgg
    14941 tttgtccatc tacatattgg ggacggctcg ttacaatttg taattcacta gatacttcaa
    15001 tcattttttc ttttatctta gtgatttcct tatcaatatc tttgatgtat aaatcgtccg
    15061 atgcacttgg gatcggaaga agcaacaatt tggattcata attgccagaa gaaagtgcac
    15121 gcagcttttc ttcgattttc ccgtattttg tcttttgaat aagtgataat aatagaaaga
    15181 caattaatcc tacacctaag gaaatggcta acaaatggaa gataagcgga acatataata
    15241 aacgtgcacg cagtaattct ccccaccaat gactttgatt gctggcataa aaataagtga
    15301 aaagtgaaaa tagaataata aggaaagccg caatccccga ataaacagct agcatcgctc
    15361 tggatatttt tcccatcata ttcgtatcac ctcgacatct ccaatcaagg tattagtaat
    15421 aatcttcaat ctacgagggt tttcatcata atcactgctg tagattttga tttgttcatt
    15481 tttcaaagaa aattgctctt cttcaaacaa gacatttcct actaatgtcg catgctctaa
    15541 tcggatggct acgcccaaag gtactaagat ccgtgttctg cctatccctt ttcttacaat
    15601 cacaatatta tcatcttttg gcaaaagcgt attgcccaaa tctatgatag tatcaccaga
    15661 aatgacggcg atgttgatat cgtcccattc atagacgtcg tttccaatac gttggttacc
    15721 gaataattgc tgtcttttcc gctcattatt atgtgttttt aattgctctg tttggatcat
    15781 catgatctgt ttctttcgcc aaaaagcatt tttggttaaa tctatccctg aaatctctac
    15841 acctttcaga ccaataaata aaacggcaaa tacagccatc atccataaag caggactgtt
    15901 gactaagcta aaaaaaatga ccaaacttcc taatatcaat tgaaaatttt gaaatttagt
    15961 acgaggatgt ttgcgcatag ctagatatat attgaaaata ccgaatatca ctaaaagcaa
    16021 aagtccagta ttgtttacga tttgccaaac agcaaataac agcagtagtg cttcaactac
    16081 cacaaaaaaa cgccaagaac tattcatcgc tctttctccc tcctgacaca ttctcatttc
    16141 atcataccta tttttttaac tgttggctat aggactttag atgtaagatc gtttgttgaa
    16201 atctaaaata cacaaagaga aaaagatcta gagtgtatca tcagtaagat gaactctcta
    16261 gatctttcag tcagactacg cttgttctac tttgacgatt ttaactaaca tatcgccacc
    16321 tggagtagca atagttactt catcattttt acgtttgcca atcaatgctt gcgcaatagg
    16381 agaatcattg gaaattttgc cagaaaacgg atctgcttca gcgcttccga caatcgtgta
    16441 ctcttcttct tctccatctg gtagttcgat gaatgtaact gttttaccaa ttgatacctc
    16501 atctgcatct acaccgtcgt tatcgatgat ttgagcaaaa cgaatcatat tttcaattgt
    16561 tgtgattctt ccttcaacaa atgcttgttc atctttcgct gattcatatt cagagttttc
    16621 ggaaagatct ccgaaaccgc gggcaatttt gattcgttca atgatttctc cgcgtttgac
    16681 tgtttttagt tcttctaatt ctttttctaa tttttcttta ccttctagag tcattggata
    16741 aactttctct accatgtgta acgtctcctt ttttcgataa aataccgcaa aaggtaatct
    16801 tttgaggaat atagtccaaa aaagattacc acgcttttct tcaaatgtaa atgtttttct
    16861 aaaaatgatc aatgtctaga gcctgttttt ctaagaatta tttacatatt tttccactaa
    16921 ttccatatgt tcttcataag ttttagcgaa ataaacattt ccagtgctcg tatctgctac
    16981 gaaataataa tagtcatttt ctgctggttc taatactgcc ttgattgcct cttcgcttgg
    17041 attgtcaaaa ggtccaggtc catagccggt attggtatac aaattatatg gtgaatctac
    17101 agctgtatct tcatacgtca ctaattcttt gtgttcacct aatgcgtata aaacagagat
    17161 atctgactga agtggcatat ttgctttgat tcggttaaag aagacttgtg caatgttttt
    17221 acgatcattt tcttttacgc cttctttttc aactagagaa gctagagtta aaacttcttg
    17281 tacagtcaaa tttttctgct tgatagctga ataatagttt tgcatgaccg tattcgtctt
    17341 gttcaccatt tcaatcacta gatccttaag cgaagtactc gtgtaataat catatgtggc
    17401 tgggaataag taaccttcca aacggtattt tgtgttctca gctttagatg cactttccaa
    17461 cagttctggg aatttttggt gcaactcgtt aaagaaagtc tcatcattca ctaaatctaa
    17521 aaattctttc ttgtctttac cagttacctt cgctacccgt tcagcaatct gatcgatatc
    17581 gtacccttct ggaatggcaa ttttcccatc tgcaactttt gttggctcag aagtaccgcc
    17641 ttcttgcaac tgtttgccaa tctcgtcaag agtcatatta ggcgctaact gataatagcc
    17701 agcttgaaag ccagttaaat ttttaaattt tgtataataa ttgaaaacaa tcccgctttt
    17761 aataatattg tccttctcca aaatctcacc aatctgcttg ttagaagagc cactcgggat
    17821 ctcgacttgg actaattggt catctgattt atctaaaggc ttcaacccgc tatctacata
    17881 acgatacacg gtaaatccaa gaaatccgcc aataatcaat aatgtaagag caatcactaa
    17941 aatgatcttc ctcacaatct tatcttcttt tttacgcaga tttctccgct tgtcgattat
    18001 atctgctgta gctgccttac tggcttcttt tttatcttta cttgggtgtt gtttgtttcc
    18061 tgacaaaaca actcctcctt actatcaagt tcgtaacttt attcattata catgatattt
    18121 gaattggcgt gaatgatttt atttaaatat tctatcaaaa atgtaagtag taaatccatt
    18181 actataagat taaaataaat gttatcaaga tacaagctat tttagcttta ttctttctgt
    18241 aaaaaagaaa ggagactaaa taaactatta tgaaaaaagg aaaacatgct aaaaaaagaa
    18301 aatattcatt tttaatgatc gttgtcgtgt tgtttagcct tagtgcgttc tctacaggaa
    18361 ctattcttgg atggcaattt tacaataatg aacactctca gatagttgcc catgctcaat
    18421 cagctcaaaa aaagttgata ctgactacca atgaacaaca gctcgttgaa aaaatcgatc
    18481 aactattaag aaaaaatgat tatattggct caatttacgt aaagaaaaat aatcgcatca
    18541 tcatggaaaa aggctatggc tatgcgaata aactcacgca acaaccaaat gatccttctc
    18601 tttattatca gattggttcg gttcaaaaag cattgactac tcttttgatt ttaaaacaag
    18661 tcgagcgtgg gcacctttct ttagatacta agctggctca attctatcca caaattgctg
    18721 gcagccagca aattactatc cgtgacttgc tgtatatgcg ctcaggcttg caccgaactg
    18781 cttctccaac cataccgatg actgatgaag aagtcgttca atttgcgatt cagcacttaa
    18841 aatttacaga ttatcagacc tctcattatg attcattgaa ctttaccata ttgactggaa
    18901 ttttgatcaa attaacaaat caaccgtatg aaaaattatt gaccgatgat atcatcgaac
    18961 ctttaggatt acagcataca aaattttatg accaagtcaa aaattcatcc aatcatgcac
    19021 tatcctatca aatgtctaaa gaggatgatt actttaaacc actgacagaa tctgaaacag
    19081 agatccataa cgaattaggg actggtaata tcagtatgtc cgtctatgat ttaaatacgc
    19141 tgtttacaca agttttatct ggaaaactag tttccaaaga cttactcttc tcattatggc
    19201 gtgaaaatgc gaatggacat ccatatagtg gtggggtata tagcggagat aattatatcc
    19261 tgtcgcaagg aaatatcgac tcttttgata catctgctat atttagcaaa gattcacagg
    19321 atgctatcat catggaaagc aatataaaag ctgacaaaac tatcaaactg cctactgctg
    19381 atctaagaaa ccaaatctat gacctgatgg aaggaactac taagttggca aatagctgat
    19441 atgatgaaaa gatagcaaat actacttctt tgaatagcat ttgcttaaaa aaagtactcg
    19501 actaaacgta ggaaagagtg tgggacaaat ctaaaaatca atttttgcta cagactttaa
    19561 ttccaaataa acagaacaga aagaggtgtt cttctttctg ttctgtccaa attattctaa
    19621 gatttctctt taccaacatt atttgacaaa gggcctatta aatctaaaat agtatcttta
    19681 gattgctaaa atatgtgcta gcaaatcatt catggcttgt tcttattttt aattttatgc
    19741 aataaaaaag aagaacaatt agcgtgatta tagaaataaa taatgagatt ttagaaaaaa
    19801 atgtgtatga atacctgatt gttactttat ttattccact ttcacttttt actgtaacaa
    19861 aaggacctcc ttgttttagt actgtagcat tttttgaatt aacagtaacc gttaatcctt
    19921 tataccggaa tacaggtaaa actattgttt tgccttttga agatttaaac gtaaatgtaa
    19981 tttcatcttt atttggagaa aagctactat aaataacttg accatcttct gtaacttgat
    20041 ggtttttcaa aagcaattga tcttttttat agcctatagg tacataatca aaattctcaa
    20101 attcttcaag tttattttcc atttgttcat tagaataagt tccttgaaaa ttcatttctt
    20161 tcaccatgtt aactactgat gaataattta aaattgaaat agctaatatt aataatagta
    20221 taaaatttct tctccttgta taagaaaatt tttccaactg tggagaaata aatagagata
    20281 ctgaaaaaga tatgaacaat gttgaaaata tcataaaacg ccaaggagct tgaataatat
    20341 ttaaaggagt attttgtaag gcaaaccatg gaaatatttt tgtacaaagt agaatagata
    20401 ttatacccat tatataagtg attttagata agattcctaa ttttttaaag ttaaatgtta
    20461 gtataattaa acaaataagc aaagttaagc caataccaaa tgatagtaat tctgaatcta
    20521 tacttccaat caaaaactct ttacccagat aaattgctct tttttgtaat atatccacat
    20581 aaggtctatt cagatcttga tacaatgtct gctcaatcat aggacctata aacgctagtg
    20641 aaagaagtaa agtaactata gcagctttta ttaatttaat aattaagaag atttctattt
    20701 tctttttccg ataaaattga ataattaaag caattataat cattaccata gtcatagcta
    20761 cagaaagaac atgagtgtaa attaataaag tcatgctaat cgccaacata acccaagatt
    20821 ctttttttga gtcgtaaagc tttataaacc cgagtaaaat aagaggaagt attgacattg
    20881 cgagtaattc ccccattgcc cctcgaagaa atatttctac actcctaaaa gaagatgttg
    20941 tatatataat agcgaaaaag gagctagtaa catgtttttt ggtaatttca tacattacat
    21001 aatgacatat aaatagagta atgagcgtaa caatataaag aaaaaggtta tagcctattg
    21061 ctaaattagt aaacttgatc aataaaaata aaggatatag agttaaccat gggtaaaaat
    21121 tatttacttg catgccatat gatctaaacg tttcaaaatt aataggggag ctaaaaatag
    21181 attctaaact taacgcacga ttaatgtgaa atgccgtatc ataaaagcca aatatttcca
    21241 tgtttttaaa tgctggaaat aaatataata aactaacaaa tgcataaact ataaatattt
    21301 ttacagaaaa aatttttttc aaattcatat accacctcat gtt
//
LOCUS       DARBEC010000050        20875 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.050, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000050 DARBEC010000000
VERSION     DARBEC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20875)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20875)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20875
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.050"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            33..329
                     /gene="yidD"
                     /locus_tag="SCL80_001695"
     CDS             33..329
                     /gene="yidD"
                     /locus_tag="SCL80_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEG1107565.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFQLTRNKKDKKEPDYKKVVKNTSSTER"
     gene            393..860
                     /locus_tag="SCL80_001696"
     CDS             393..860
                     /locus_tag="SCL80_001696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1107566.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVAPTYQGKGIGGKLLETAFQKLSQLASSQTQIIIRTGSTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            945..1829
                     /locus_tag="SCL80_001697"
     CDS             945..1829
                     /locus_tag="SCL80_001697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1107567.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKAQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLN
                     GDKTFSKLGTAVQMAWRYCERKGLDKNSYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLVGAAANYLAVRQGEN"
     gene            1943..2179
                     /locus_tag="SCL80_001698"
     CDS             1943..2179
                     /locus_tag="SCL80_001698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEG1107568.1"
                     /translation="MMSKKNKDVEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            2267..2347
                     /locus_tag="SCL80_001699"
     tRNA            2267..2347
                     /locus_tag="SCL80_001699"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2301..2303,aa:Tyr,seq:gta)
     gene            2354..2425
                     /locus_tag="SCL80_001700"
     tRNA            2354..2425
                     /locus_tag="SCL80_001700"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2386..2388,aa:Gln,seq:ttg)
     gene            2550..2720
                     /locus_tag="SCL80_001701"
     CDS             2550..2720
                     /locus_tag="SCL80_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107569.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            2940..3647
                     /locus_tag="SCL80_001702"
     CDS             2940..3647
                     /locus_tag="SCL80_001702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1107570.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRGDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(3707..4351)
                     /locus_tag="SCL80_001703"
     CDS             complement(3707..4351)
                     /locus_tag="SCL80_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEG1107571.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKSFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSADHFKESIIELLNGQVQFEEGPVSYHEQLVTDHI"
     gene            4478..4765
                     /locus_tag="SCL80_001704"
     CDS             4478..4765
                     /locus_tag="SCL80_001704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107572.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            4873..5193
                     /locus_tag="SCL80_001705"
     CDS             4873..5193
                     /locus_tag="SCL80_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1107573.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIT
                     LIGE"
     gene            5274..6587
                     /locus_tag="SCL80_001706"
     CDS             5274..6587
                     /locus_tag="SCL80_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1107574.1"
                     /translation="MNHFWGRRLLTRDIKAIDCEEVNLPNYEKIAAVERLGNWILCHR
                     CGMRTSVIDGQLADYGYFCTHCLYLGRCDTQQDLYLFDQPEAKPREVVFFWTGQLTEK
                     QTEIAKRILCHSEKRHHLIWAVTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNEL
                     FLRYRYVFPEEKITLLHGNTATAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYN
                     GDAGLAHAVQTAIKPDGRFIYLSATPDKKLLKEIKQTFDIHKLALRFHRRLLPEPVLV
                     FMNNWRQKCKNKKKIKSLVRIIHQLIKENHILLFCPSITMMHRLKATLQEALPRYEIT
                     DVSSRDNQRAEKVQKMREAKYDILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQ
                     IAGRADRKGPYTNSQVYFFYEEKTAAIRKACQEIKEMNEEGKKML"
     gene            6584..7303
                     /locus_tag="SCL80_001707"
     CDS             6584..7303
                     /locus_tag="SCL80_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEG1107575.1"
                     /translation="MRCSCCSKDIVQNLTFSEIIGVSKYRLPQTCSVCRAQFHLLETR
                     GCSGCQKPIHFNLESTKKKVKSIPVYCSDCQEWKKKYPTYDFQHRAFFVYDENMKAWL
                     NQYKFMGDIRLAGTFAYKWTQIKKEYPTYIYCPIPLSEKRRTERGFNQVSEMLKAARI
                     PFQMLLKREKHLLAQAKNTRKERLEMPQPFELAVTFSKIQNKKILIIDDVYTTGQTLF
                     HAADCLQVAQPKIIRTISLAR"
     gene            7431..7982
                     /gene="raiA"
                     /locus_tag="SCL80_001708"
     CDS             7431..7982
                     /gene="raiA"
                     /locus_tag="SCL80_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEG1107576.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADATALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
     gene            8204..10738
                     /gene="secA"
                     /locus_tag="SCL80_001709"
     CDS             8204..10738
                     /gene="secA"
                     /locus_tag="SCL80_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEG1107577.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPHHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKSFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
     gene            10864..11965
                     /gene="prfB"
                     /locus_tag="SCL80_001710"
     CDS             join(10864..10935,10937..11965)
                     /gene="prfB"
                     /locus_tag="SCL80_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEG1107578.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            12070..12756
                     /gene="ftsE"
                     /locus_tag="SCL80_001711"
     CDS             12070..12756
                     /gene="ftsE"
                     /locus_tag="SCL80_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEG1107579.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            12740..13633
                     /locus_tag="SCL80_001712"
     CDS             12740..13633
                     /locus_tag="SCL80_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107580.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEKAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            13785..15233
                     /gene="cls"
                     /locus_tag="SCL80_001713"
     CDS             13785..15233
                     /gene="cls"
                     /locus_tag="SCL80_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1107581.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWVLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGKIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHSTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            15570..16427
                     /locus_tag="SCL80_001714"
     CDS             15570..16427
                     /locus_tag="SCL80_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEG1107582.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            16542..17462
                     /gene="pstC"
                     /locus_tag="SCL80_001715"
     CDS             16542..17462
                     /gene="pstC"
                     /locus_tag="SCL80_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEG1107583.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            17462..18346
                     /gene="pstA"
                     /locus_tag="SCL80_001716"
     CDS             17462..18346
                     /gene="pstA"
                     /locus_tag="SCL80_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEG1107584.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            18361..19167
                     /gene="pstB"
                     /locus_tag="SCL80_001717"
     CDS             18361..19167
                     /gene="pstB"
                     /locus_tag="SCL80_001717"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1107585.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            19183..19941
                     /locus_tag="SCL80_001718"
     CDS             19183..19941
                     /locus_tag="SCL80_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEG1107586.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGIDLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            19954..20631
                     /gene="phoU"
                     /locus_tag="SCL80_001719"
     CDS             19954..20631
                     /gene="phoU"
                     /locus_tag="SCL80_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEG1107587.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
BASE COUNT         6903 a         3574 c         4572 g         5826 t
ORIGIN      
        1 tttttagtaa aagaaaagaa ggaatctaat tgatgaagaa gatattgatt tatttagtta
       61 gaggatatca gcgctttatt tcgcctgcat ttccacctag ctgccgttat tacccaacct
      121 gttccaatta tatgataaaa gcaattcaaa cccatggtgc actaaaaggg acggtaatgg
      181 ggactgcacg gatcttacgc tgtcatcctt ttgtcaaagg cggtatcgat tatgtaccat
      241 tgacattcca attaacgcga aataagaaag ataaaaaaga accagattat aaaaaggttg
      301 tgaaaaatac gagtagcact gagcgataaa gaaaaagagg aaaattattg tcaaagtaac
      361 aactccgcaa ctacttatat gggggacaaa aaatgatcga agaactaaac aagaaagatt
      421 taccatggtc cttgttatta gatgcagatc ctgataaaga aaaagttcaa gtatatgttt
      481 ccagagggag tggctggata tggaaagaaa aagaaaaaac aataggtgta ctgatctatg
      541 tggcaaggga aaaagaatac gaaatcgtca atgtagcagt agccccaacg tatcaaggaa
      601 aggggattgg cggaaaatta cttgagacag cttttcagaa gctttcacaa ctcgcttcca
      661 gtcaaactca gatcatcata cgaaccggca gtactagcag tgcggcgtta catctctatc
      721 aaaaaatggg ctatgtggag attggtcgag tcaaagacta ttttatccat cattactctg
      781 agcccatttt tgaaaatgga gatcaattaa gggatcaagt aattttagca attcacttac
      841 ctaaaaatag ctttaactaa taaactttca attcgagaaa atgctttcaa atgctaaata
      901 gatacattac aatagcttta gcaaacaaaa aggagaatat ttttatggga atattagttt
      961 ttgattttgg tggatcggct gtcaaatatg gttgttggga tggcaaagag attaaaggta
     1021 agggacaatt tgcaacgcct gaatcttggg aagaaatgaa agcgcaatta tttcaagttt
     1081 ataaaaaaat actcgtttct tttgaagtcg aaggaattgg aatcagctct cctggagtag
     1141 tgaataatga gaaacaggtg attgagggaa tcagtgcgat tccttatatt catggattta
     1201 atattttccg agatttggaa actctgtttc aactcccagt aacgattgaa aatgacgcca
     1261 actgtgccgg aatggctgaa ttttatgaag gtgctgcccg tgattatcaa gatgttgctt
     1321 ttgttgtggc aggaactggg attggcgggg cgttgttcac gaaaggacaa atcaataaag
     1381 gggcacatct ttatggtgga gaatttgggt tgatgttctt aaatggcgat aagacattca
     1441 gtaaacttgg tacagcggta cagatggctt ggcgttattg tgaacgcaaa ggtctcgata
     1501 aaaattctta taccggaaaa gatgtctttg aattagcaga aactggagat gcaatagcaa
     1561 aagaagaagt tgagagtttt tatacgtatc ttacaaaggg attattcagt attcaatttt
     1621 cgtttgatcc agaagtgatc gtattaggcg gcggtgtttc tgcaaaagaa gggctcattg
     1681 atgagatcaa aagccgaatg aagaagctga cagaacaatt cgatctgcat gatttcgaac
     1741 cgcagatttt attatgtgaa tacagaaacg atgctaatct tgtaggtgca gcagcaaact
     1801 atctcgctgt acgtcaaggg gagaattaag agctttagag aaggtgcagc ttgcataaaa
     1861 gaagtgaatg aaatgaatag gagattttta cctaaggaat gttttatttg ctgcagtatg
     1921 ataaaatatc tgagaggtga aaatgatgtc aaaaaaaaat aaagatgttg aagtgcgtat
     1981 agaagaagtg aaaaaagata tcaaaggtaa gaattatgaa gtcactcagt tatttatagg
     2041 gaaaaaaatg attggtgaga tcttagccta tgggccaaaa gaatatgaaa ttttctttgg
     2101 tgaagaagat tttggaaaag aaaaaagtct tgaaaatgcg atagaaacag tgattcgaca
     2161 ctggaatctg cacgaataaa aaaatatcaa aaaaactttc aataaagggt tgcaaaaatc
     2221 tggaaaatta ggtattataa ctaagtcagg ttgatacaaa cctttcggag gagtagcgaa
     2281 gtggctaaac gcgacggact gtaaatccgt tccttagggt tcagtggttc gaatccactc
     2341 tcctccattt cattggggta tagccaagcg gtaaggcaag ggactttgac tccctcatgc
     2401 gttggttcga atccagctac cccagtatac cacaatcgta agctctcgtt agttcgagag
     2461 ctcttttttt tgctttgcta ccttgttcct tgaagattct gctgatttaa gatagagtta
     2521 agttaactac aagaattgga gggatccaca tgaaatggaa agaaaaagta gacgaagcag
     2581 ctgaaaagtt atatgatttg gtcaaatcag aaaaatacaa tattgaagtg aatattccga
     2641 aaaaaggcgg aaaagcggtc caggtaaaat caaaacgccc aacgaatcat acaaaaaaat
     2701 ggatggctaa aaaccgctaa taataacaaa agatccataa gtcggcattt tcatcgattt
     2761 tagggtcttt tgttattttc taggttgaac atatctaaaa aattgtttct gacaatagga
     2821 gtaggaagaa cgatgtgtgt ctaaatcctc tgttgatgga tagaaacctt tatctttttc
     2881 agttgtgaat caggtatata ccagttataa tagaagaaac gaaaggacgg gtgtacgtta
     2941 tgtcgaatca attacctgta tatatacaga ttcatgacca attaaaaaat gaaatcgaac
     3001 aaggtatatg gaaagtcgga gaccgtcttc cttcagaaag agaattagca gtgaaattca
     3061 atgtcagccg tatgacgttg agacaagcaa ttcaaaactt ggcagatgaa ggaatcttag
     3121 aaagaaagat cggttcgggt acatatgttg cgcgagaaaa agtacaagaa aaaatgtctg
     3181 gtgcaacaag ttttacagaa attatggaat cgcaaaatcg agtaccgtct agtcggacaa
     3241 tctcttattt cttgacttcg ccaagctcaa gtgaaatgga aaaactaaac ttgagtaaag
     3301 atgacacgat cttgcgaatg gaacgtattc gtttcgcaga tgatgtccct atttgttttg
     3361 aagttgccag tgtgccacaa aagctgatcg atggctacag caaagcagag attactgaat
     3421 cgttgtatcg cacgctagaa gaaaaaggcg ggaaaaagat cggtccagcg aatcaaacga
     3481 ttactgctgt gttggcttct gagaaaattg ctgactattt agaggtaaaa cgaggagatg
     3541 cagttcttcg tttgcgccag atttcttatt ttgaagacgg tacgccgttt gagtatgtgc
     3601 gtacgcaata cgtaggaaat cgttttgagt tttatttgga aaaataaaaa acaggtgatc
     3661 cctgattcct tacttagaaa taaaagagaa tcagaatcac ctgtttttat atatgatcag
     3721 ttaccagttg ctcgtgataa gaaacaggac cctcttcaaa ttggacttgt ccgttcagca
     3781 gctcaatgat ggattcttta aagtggtcag cacttttttc atctacagca cagatgacgc
     3841 taacttgatc agtatattgg atttctttta taaaaaaatg ttggagttca agataattct
     3901 gtaattttcc tagttgcgga tacgtgatca tcacagagat ctcctgttgc aaggtacctt
     3961 ctacgattcc aatgtcagac aatgcttgag agacagcatg agtgtatgct cgtattaatc
     4021 cgcctgtgcc gagtttcgtt cctccaaaat atcgagtgac tactgcagct acattgatca
     4081 gttgattttt tttgaggact tccaacatcg gtacgccagc cgttccactt ggttctccat
     4141 catcgctgct tcgctggatt tcattttttt ctccaatgat gaaggcgctg cagttatggt
     4201 tcgctttcca atgttctttt tttatttctt gaatgaaact ttttgcttgg tcctctgatt
     4261 ctattcgtct aagagaacag ataaagcgtg attttttgat ctcaatctca ctttgtccat
     4321 tttcttttat tgtacgatag cgctcgatca tcttgtcacc tctttttttc attttcttta
     4381 ttcagtataa ataaagttct aggttgtttc aacactgtaa cggttgcgca atcgctgtag
     4441 tatgcgataa taaagacaaa ggtaggtgga tctggggatg caggaacgaa tgaaaaagta
     4501 cgatgcaatt acgcattatt tgaagaacaa tggcggctct caagtaacgt tgacctttac
     4561 ccaatttgac gaactgctgt ttcccagtaa cggacttccc aaaacagcaa gggaaagtac
     4621 agattggtgg gcaaatgatt ataaacaccc tgaaaaaggt gcctatggct ggataaatgc
     4681 aggctatgaa gtagttgtca tcaatttgga caaagaatat gtcgtgttca ataaattagt
     4741 gaaatctagt tggctatttg attaaataaa ccgcgtttag aaaacgttga caaatttctt
     4801 tctttttata taatagagta acagacgcga agaaaacgtt tgcgtagttt gttttaaaaa
     4861 ggagagaaaa gtatggctaa aaaaacaatc atgcttgtat gttcggctgg tatgagcaca
     4921 agtttactag taacaaaaat gcaaaaagct gctgaagcaa aaggaatcga ctcggatatt
     4981 ttcgctgtgt ccgcttctga cgcagataac aatcttgcaa ataaagatgt agatgtactg
     5041 ttattaggac ctcaagttcg ctttatgaaa gctgattttg aaaaacgtct ggaaccaaaa
     5101 ggcattcctt tagacgtcat caatatggct gattatggta tgatgaatgg cgaaaaagtg
     5161 ttagatcaag caatcacgct cataggtgaa taaatagaac agtataacta tcgaacaagc
     5221 gatccaaaaa atagatcgct tgttttttcg tatatttttt tagggcgaat aatatgaatc
     5281 atttttgggg tagaaggctt ctgacgagag atattaaagc aatagactgt gaggaggtaa
     5341 atttaccaaa ttatgagaag atcgcagcag tggaacgttt gggcaactgg attctttgtc
     5401 atcgctgtgg aatgaggacg tccgtcattg atggacagct tgcagattac ggttattttt
     5461 gtacacattg tctatattta gggcgatgcg acactcaaca agatttatat ttgtttgatc
     5521 agcctgaagc aaaacctcga gaagtcgtct ttttttggac aggacagctt actgaaaaac
     5581 aaacggaaat cgcaaagaga atactttgcc attcagaaaa aagacatcat cttatttggg
     5641 ctgtaacagg tgcagggaaa acagagatgc tttatcctat tttagtggaa actttaaaag
     5701 ctggaggaag agtagcgatt tgtacaccgc gagtcgatgt ttgtaatgaa ttgtttttac
     5761 gctatcgtta tgtttttcca gaagaaaaaa tcacgttgct ccatgggaat acagcaacgg
     5821 cttatacctt tacttctttt gttatctgta cgattcatca gctataccgt ttttatcggt
     5881 cctttgattt aatagtgatc gatgagatcg atgcgtttcc ttataacggg gatgcaggat
     5941 tagctcatgc tgtgcaaaca gcgattaaac cagacggacg attcatctac ttaagtgcca
     6001 cacctgataa aaagttacta aaggaaatca agcagacgtt tgacatccac aaacttgctt
     6061 tgcgctttca tcggcgttta cttcctgaac ccgtcttggt tttcatgaat aattggcgac
     6121 aaaaatgcaa gaacaagaag aagataaaat ctttggtgcg gatcattcat caattaatta
     6181 aagaaaacca tatcctttta ttttgtccaa gtatcacgat gatgcatcgt ttgaaagcca
     6241 cacttcaaga ggctcttccg agatatgaga tcacagatgt ttcttcaaga gacaatcagc
     6301 gggcggaaaa agtccagaaa atgcgagaag caaaatatga catactgtta accacgatga
     6361 tccttgaacg tggagtcaca tttgaaagaa tatccgttat cgttttagga gcggatcatc
     6421 cggtttttac taaatcttct ttggtacaaa tcgcaggaag agccgatcgt aaagggcctt
     6481 atacgaacag tcaagtatac tttttctatg aagaaaaaac tgcagctatt cgaaaagctt
     6541 gtcaggaaat caaagaaatg aatgaagaag ggaagaagat gctgtgaggt gcagttgctg
     6601 cagtaaagat atcgtccaaa atttaacatt cagcgagatt attggtgttt cgaaatacag
     6661 attacctcag acttgttcag tttgtcgcgc acaatttcat ttattggaaa caagaggctg
     6721 ttctggctgt caaaaaccaa tccatttcaa tctagagagt acgaaaaaga aagtaaagtc
     6781 tattcccgtt tactgcagtg actgtcaaga atggaagaag aaatatccga cctatgattt
     6841 tcagcaccgt gccttttttg tgtacgatga aaatatgaag gcctggctca atcaatacaa
     6901 gtttatggga gatatccgac tagcaggcac ctttgcatat aaatggacgc agataaaaaa
     6961 agaatacccg acatacatct attgtccaat acctttatct gaaaaaaggc gaacagaaag
     7021 aggattcaat caagtaagcg agatgttgaa agcagcaaga attccttttc agatgctact
     7081 caaaagagag aagcatctgc tggctcaagc aaaaaacacg cgaaaggaaa gattggagat
     7141 gcctcaacct tttgaacttg cagtcacctt ttctaagata caaaataaaa aaatattgat
     7201 tattgatgat gtctatacaa caggacagac tcttttccac gcagcagatt gtttgcaggt
     7261 cgcacagccg aaaatcatcc gaaccatttc tttagcaaga tgaattagat gtggaaattt
     7321 gatagcgatt taatttgcaa ataacctaga gttcgatata atagaattaa gaagagggaa
     7381 agagtggtcc ttctccttct tgattgtggt agacgaaagg ggtaattttt atgtttagat
     7441 ataatgtacg cggcgaaaat atcgaagtaa ctgaagcgat tcgcgactac gttgagaaaa
     7501 aagtaggcaa actagaacgc tatttcagtg acgcacccga tgcaacggcg catgtgaact
     7561 taaaagttta tacagaaaaa acagcaaaag tggaagtaac gatcccatta ccttatcttg
     7621 tattacgtgc cgaagaaact tctccagatt tatacgcaag tatcgatttg gttgttgaca
     7681 aattggaacg ccaaatccgc aaattcaaaa caaaaattaa tcgtaaatcc cgtgaaacgg
     7741 gcatgaatac tgcagatgct actgctttat tcaatgaaga ggacgaaaac gaaaatgatt
     7801 ctgaattaga tatcgtacgt acgaaacgcc tttcattgaa accaatggat agtgaagaag
     7861 cagtcttaca aatgaacatg ctgggacata acttctttat ttttgaagat gcagaaacaa
     7921 atggtacaag tatcgtttac cgccgtaaag atggaaaata cggactgatt gaaacagact
     7981 gatcagcctt acttaaataa caacgaaaac ttgcagcagc tactgacagc tgccgcaagt
     8041 ttttttgagt ttcctcctct tttagatgaa atatgaataa attatgaagt tcttacttta
     8101 atacgcttaa gatatttcaa ttatctataa ctaatgataa aatgaaaagt aaagtctaca
     8161 tagtctggat aatacacaga aggaaaagga gattacacaa cgcatggcca atttgattcg
     8221 ttcacttatc gaaaacgata agaaagaact aaaacgttta gatagcattg caaaaaaagt
     8281 tgaagcatac gctgaccaaa tggcagcatt aactgatgaa caattacagg caaaaactgc
     8341 tgaattcaag ggcagatacc aaaaaggaga aacattagat caattacttc cggaagcttt
     8401 tgcagtcgtc cgagaagcag caaaacgtgt attggggtta tatccacacc acgtacaatt
     8461 gatgggtgga atcgtgcttc acgatggaaa tatccctgaa atgcgtactg gtgaaggtaa
     8521 aaccttgacc gcaacgatgc cagtatatct aaatgctctg actggcgaag gggtacacgt
     8581 agttaccgtc aatgaatatt tagcaacccg tgactcgacg gaaatgggcg aactttataa
     8641 tttcttagga ttaacagtgg ggttgaatat caactcaaaa tcttcggatg aaaaacgcga
     8701 ggcttataat tgtgatatca cttatagtac gaataatgaa ttaggatttg actacttgag
     8761 agacaatatg gtcgtttacc gccatcaaat ggtacaacgt ccgctgaatt atgccattgt
     8821 cgatgaagtt gactcaatct tgattgatga agcgagaacg ccgctgatca tttcagggca
     8881 agcagaaaaa tccacagctt tatatacaag agcagacaac ttcgtgaaac gtttgaaaga
     8941 agaagaagat tataaaatcg atgtacaatc aaaaacaatc agtttaaccg aagcagggat
     9001 cgaaaaagca gaagaaacat tcggtttgga taacttatac gacattgaaa atactgcatt
     9061 gacccatcac ttggatcaag cattgagagc caactatatc atgcttttag atatcgatta
     9121 tgttgtccaa gacggaaaag tactgattgt tgaccagttc actggtcgga tcatggatgg
     9181 acgccgttat tcagatggac ttcaccaagc aatcgaagct aaagaaggcg tagagatcga
     9241 agacgaaacg aaaaccatgg cgacgatcac tttccagaac tatttccgta tgtacaagaa
     9301 acttgcggga atgactggta cagctaaaac agaagaagaa gaattcagag aaatttataa
     9361 catccaagta taccagattc cgacaaatcg tccggttatc cgtgatgatc gtccagattt
     9421 gctttaccca acattacaaa gcaagttcca cgctgtcgtg caagatatca aagatcgcta
     9481 ccgcaaaggt caacctgtgt tagtcggaac tgttgcggtt gaaacatctg agttattatc
     9541 agatatgctg aatcaagaac gtattcctca tgaagtgctg aatgcgaaaa atcactttaa
     9601 agaagcagag atcatcatga atgctggtca aaaaggtgct gtgacgattg ctaccaacat
     9661 ggctggtcgg ggaacagata tcaagttagg acttggggta agagaacttg gtggtctagc
     9721 agttatcggg acagaacgac atgaatctcg tcgtatcgat aaccagcttc gaggacgtgc
     9781 aggacgtcaa ggagaccctg gtatgtcaca gttctacttg tcattggaag atgatttgat
     9841 gaaacgattc ggttcagaac gaatcaaatc tttcttagat cggatgaaga tcggtgagga
     9901 agatgctgtt atccaaagca agatgctgac gaaacaagta gaatctgcac aaaaacgtgt
     9961 agaaggaaat aactatgaca cacgtaaaaa cgtcttacaa tacgatgatg tgatgcgtga
    10021 acaacgtgaa gtaatctata aacagcgaca agaagtaatc atggaagaaa aaagcttatc
    10081 caagccacta atgaacatgg tcaaacggac aatcagtcgt gtagtagatg cgcatacaca
    10141 attagagaaa gaaaattgga atttggaaag tatcatcgat tttgccggca gtactcttgt
    10201 ccatgaagat agtattactc ttgcagatat cgaaggaaaa acagctcagg agatcaaaga
    10261 gtatctaaac gaacgagcac aagaagtatt taataccaaa gcggctcaac taaatggtcc
    10321 agagcaactg ttggaatttg aaaaagtcgt gatcttgcgt gttgtggact ctaaatggac
    10381 agaccatatt gatgcaatgg atcaattacg tcagtcaatc ggcttacgtg cttatggaca
    10441 gaacaatcca ttagtcgaat atcaaacaga aggctataag atgtttgaag atatggtagg
    10501 cgccatcgag tatgaagtca ctcgaatctt tatgaaagct gagatccgtc aaaacgtaca
    10561 gcgtgaacag gtttctcaag gaacagcttc tcactcagaa gacggagatg ccaaagaaaa
    10621 tagtaatact agcgcgaaaa aacagccaat ccatgtgaac aaggtcggaa gaaatgatcc
    10681 ttgtccttgt ggaagcggga aaaaatataa gaactgtcat ggaaaaaatg caaactaatt
    10741 tctgacagca ggattggtga gacaaattct ttttttaaga attcgtctca ccttctttct
    10801 gttatttgct gattctgtta gactagatat aatgaaaaaa gtatagtgaa agaaggaaaa
    10861 aacatggaaa tatccgaaat tcgaaatctc ctgacagaaa tgcaggaaaa ggtcacaagc
    10921 ttcaggaggt ctctttgact tagaccagct ggaagaagac attgcggaag cagaagcaaa
    10981 aatggctgaa cctggttttt gggatgatag cgaagctgct caaaaagtga tcagtgaaaa
    11041 caatgcaaat aaagaaactt atgatcaatt ccattctctt gctgaagaac tggaagaaat
    11101 cgaattgatg gtggagatgt tgcaggaaga acctgatgca gccatgcaag aagaagcaga
    11161 aacacgtatc aatgcgctag atgaaaaaat gaagacatat gaattatcta tgttgttaga
    11221 tggtccttat gatcgaaaca acgccatcgt tgaattgcat ccaggggctg gcggaacgga
    11281 gtcccaagat tggggcagta tgcttttacg tatgtataca cgctgggcag agcagcatgg
    11341 gtatcaagta gagacactag attatcaagc aggtgatgaa gcaggaatca agagtgtgac
    11401 cctactgatc aaaggacaca acgcatttgg ttatctgaaa tcagaaaaag gtgttcatcg
    11461 ccttgtacgt atctcgcctt ttgactcagc gaaacgacgt catacttctt tttgttcggt
    11521 agatgtgatg ccagagcttg atgaaacgat cgatatagaa atcaatccag atgatttgaa
    11581 aatcgatacg taccgagcaa gtggtgcagg tggtcagcac atcaataaaa cagaatctgc
    11641 tgtacggatc actcacttgc ctacaggaac agtagtagct agtcaggcac aacgttcaca
    11701 attgaaaaac cgtgagcaag cgatggggat gttaaaagct aagctttatc aattagaagt
    11761 ggaaaagaaa gaacaagaag cagctgcact tcgaggagaa caaaaagaaa tcggctgggg
    11821 ttcacagatc cgttcttatg ttttccatcc atattctatg gtaaaagacc atcggacaaa
    11881 cgtagagaca ggaaatgtcc aagcagttat ggacggtgac cttgatttat ttattgacgg
    11941 ctatttaaaa atgcatctac aataagaacc cattgttaaa ttgaaataaa attgtaagat
    12001 tttgcaagca tgttgaaaca ttgccatgct ataatagcta aagactgaaa aagatatgga
    12061 gagaacgcca tgattgaaat gaaggatgta atgaagaagt actcaaatgg tacgaccgct
    12121 atccgcaatc tctcaattgt catcgaacaa ggagagtttg tctatgtggt tggtccgtct
    12181 ggagccggta aatctacatt catcaaattg atgtatcgag aagaaaaagc ttcaaaagga
    12241 actttgaacg tcgcaggtca tgatctaata gcaatcaaaa accgcgaagt tccttatcta
    12301 cgcagagaaa tcggcgtggt gttccaagac tataaattat taccaagaaa aacagtatat
    12361 gaaaatgttg cgtacgctat gcaagttatc ggcagaaaac ctcgggatat caaaaaacga
    12421 gtaatggaag tgctggacct tgttggtttg aaacacaaag ttcgagtctt tcctagcgaa
    12481 ttatccggtg gggaacagca acgtgtctct attgctcggg caatagtgaa tacgcctaaa
    12541 gtacttatcg cggatgaacc gacaggaaat ctggatccag aaaactcatg ggaaatcatg
    12601 aaattattag atcggatcaa tgcccaagga actactgtcg tgatggctac acataacagt
    12661 acgatcgtaa atacgattcg gcaccgagta attgctatcg aaaacggccg cattattcga
    12721 gatcaagcgg aaggggaata tggatacgat gattagaacc tttttccgcc atttattaga
    12781 aagtatcaag agtttgaaac gtaacggctg gatgacagtt gcttcagtca gtgcggtaac
    12841 gatcacattg actttagtcg gtgtattcat ggcagttatc atgaacgcaa ctaaattagc
    12901 tcaagatata gaaggaaacg ttgatgtctc tgtattcgta gatatcggta caaaagacga
    12961 agagatgaaa aagctagaaa aagaattgaa tgacttagat catgttaaaa aagttgaatt
    13021 ttcaagcaaa gaacaggaat tgaaaaaaat ccaaaacgaa atgggtgatt cctggaaatt
    13081 gtttgaaggt gacaataacc cgttatatga tgtgtatgta gtaagtgcta ctgatccttc
    13141 ttatacaaaa caagtagcag aaaaagcagc agatttgaag aatgtttctc gttctgatta
    13201 cggtggagct tcctcagatc ggattttcca aatcgctggt gcagttcgga catggggatt
    13261 agttgcagcg gcattgctgc tgttcgtagc gatgtttctc atttcgaaca cgattcggat
    13321 cacgattttg tctagacagc gtgaaattca aatcatgcgt ttagtcggtg caaaaaatgg
    13381 atatattcgc tggccgtttt tccttgaagg tggctggatc ggtctgatcg gtgcgattct
    13441 tccgattttg atcatcacat ttggttatgc atggtttttc caattgatca atccatcatt
    13501 attacgttcc cattattcat tgattcatcc acaaaatatc gtttggaaaa tcaatctttt
    13561 aatggtcggc attggtgtga tcattggttc acttggctcg atcatttcta tgagacgttt
    13621 cttgaaattc taataccaca atataaataa aatgagaaag ttggacagtt tcgttccagc
    13681 tttctttttt tattggctag gagaatagaa ggaagcggct tttttccctt tactttaaaa
    13741 aacaacttgg gtattcttaa gttggtagaa gaagaggtga agccgtgaaa attatcgttg
    13801 acaatttttt tacgatcctt ttgattatga atattttgct ttcctttatc atcgtttttc
    13861 gagaaagaaa ggaaactgct caaacatggg catgggtgct cgttttgatg ttcattccag
    13921 ttgtaggatt tattttatat atttttttag gaagagggat ttccaaagag aaaatctttg
    13981 atttgaagat ccaagggaaa atcggtaaga acctggaaat tgaagaagac aaacaggcat
    14041 tgatgcgagg gctttatcct catccgccta ctggacaagt agatgtgaaa caattgatct
    14101 atatgctcac ggtatttgaa agtacgctct atacgactaa aaatgaaatc actttattta
    14161 cagatggtcg tgaaaaattc gatgcgctga ttgaagatat caagcaggca gaagaccata
    14221 tccacttcca gtattatatt tatcgcagtg atgcattagg tgaagaagtt cgagatgcat
    14281 tgacagaggc ggcaagacga ggggtaaaag tcagagtcct tcttgatgca tggggttcca
    14341 cacaagtttc ttccagcttt tttcagaatt tgaaaaaagc aggcggagaa gtcgcatttt
    14401 tctttcctct atttgttccg tatattaatc ctcggatcaa ttatcgcaat catcgtaaaa
    14461 tcgtagttat tgatgggaaa atcggatata caggtgggtt caatgttgga aatgaatatc
    14521 taggattggt aaagaaattt ggttattggc gagacaatca tttgcgtatt tacggagaag
    14581 cggtgtatac cttgcaaaac cgttttttga tggactggaa ttctcagcat acaaaagaag
    14641 taggctattc tcccaaattt tttccgtcca ttcactctac tggtgatata gctgttcaaa
    14701 tcgtgacaag cggaccagat accgaacatg aacaaatcaa aatgacttat ttgaaaatga
    14761 tttcgttagc caaacgggag atattgatcc aaacgcctta ttacataccg gatggatcga
    14821 ttcatgaagc actgaagttg gctcttcttt caggcgtaaa agttcatatc caaataccta
    14881 acaaaccaga tcatcttctc gtttactggg caacgtattc tttcgctgca gagctaatcg
    14941 agtatggagc aaggatcgaa acatatgaga atggatttat tcatgcaaag acgatgatta
    15001 tcgatggtgg cattgtttca gttggctcag caaatatcga tgttcgttca tttcgcttgg
    15061 actttgaggt caatacactg gtttatgatg aacgaatggc ttcacgtgta agaaaagctt
    15121 tttttgaaga ctcgaaaatt tctacgcatc tcacaaaaga aatgtatgaa aaccgaggaa
    15181 taatcatcaa gatgaaagaa ggattagcaa gattgatttc gccgctcctt taaaacataa
    15241 aaaggacatg agaaccatca acagatggag atctcgtgtc cttttctaac ggggccattt
    15301 ctgagagtac gtcagaaatg gagaaagcgt ctcgcatgag cgaaattaac tttggggtat
    15361 ctcttgtttt ttggggaaag caagaaataa gctaatatat gctattaaaa gagacacatt
    15421 tcaaagccag tatagcatag aaacttttaa tgagatagtt ttttttacaa ttatgagaat
    15481 ttattttgtt atttaatgat tttgattcgt tttcatcaag taatgaaaat gataaaataa
    15541 acatacttta ggattaaagg agatttctaa tgaaaaaact taaattattc ttaccaataa
    15601 taggattagc tattctttta tcaggctgtt caaagtggat tgatcgagga gaatccatta
    15661 cggctgttgg ctcttctgct ttacagcctt tagtagaaac agttgctgaa acttaccaga
    15721 cagaaaatcc tggaaaattc atcaatgtac aagggggcgg aagcggaact ggtttgtctc
    15781 aagtacagtc aggagcagtc gacatcggaa actctgattt gtttgcggaa gaaaaatcgg
    15841 ggatcgatgc ctcagcatta gtcgatcatc gagtagcagt tgttgggatc actcctattg
    15901 tgaacaagga tattggcgta aaagacatca gtatggaaaa cctgaaaaaa ctatttacag
    15961 gcgagatcaa aaactggagt gaagtcggtg gaaaagatca gccagtagtt atattgaacc
    16021 gcgcttcagg aagcggaacc cgtagcacgt ttgaaaaatg ggtattggac ggagaaacag
    16081 ctgttcaggc acaagaacaa gattcaagcg gaatggtgcg ccagatcgtt gccgacacac
    16141 ccggtgcaat cagctatgtt gctttttctt atgtttccga tgatgtagat acgttgaatg
    16201 ttaacggtgt agaaccgaca gatgaaaatg tgacaacgaa caaatggacg atttgggcat
    16261 atgagcatat gtacacaaga gatcatccaa aaaaactgac aaaagaattt ttagactata
    16321 tcttatctga tgaagtacaa gataacatcg ttggagaact aggttatatc cctgtttctc
    16381 atatgaaagt cgaacgtgat tggcaaggaa atatcgttaa agagtaatct ttacacaaaa
    16441 tttacaaact tagctaaact tttacatgtt attaaaacca aattgatgtt caatttgtac
    16501 actgagtgag tatgttaaac ctgattaagg gggagtcatc tgtggaagat gtgaaaaaag
    16561 tattattaac aaaatcaaaa cgttcaaaaa tggaacagcg tggaaaattc atcagttttt
    16621 tatgtatcgc gctgatcgtg ttggttgtat tatctatttt ttattttgta gctagtaaag
    16681 gattggccac cttttttact gataagatca atgtatttga ttttctgttt ggtacagatt
    16741 ggaatccaag ccaaatcgga gcaaatggga aaccaatggt aggggcattg cctatgatca
    16801 caggttcgtt tattgtgacc tttttatctg ctatcgttgc cacgccattt gccattggtg
    16861 cagcagtatt catgacggag atctctccta aaaacggaac gaagatctta cagccggtca
    16921 tcgagctgct tgtaggtatt ccttctgtcg tttatggttt tatcggttta tcagttatcg
    16981 tgccttttgt cagaagtatt tttggcggga ctgggtttgg tatcttagct ggtacatttg
    17041 tgttgttcgt tatgatcttg ccgactgtca caacgatgac tgttgatgca ttaaaggcag
    17101 tgccacggca ctatcgggaa gcttcattag cgcttggtgc cacaagatgg caaacaatct
    17161 acaaagtcgt gctacgagca gccgtgcccg ggattttaac agcagtcgtt tttggtatgg
    17221 ctcgtgcgtt tggtgaagcc ttagctatcc aaatggtcat cggtaatgca gcattgatgc
    17281 caacaggtct tactactcct gcttctacat tgacgtccat cttaacaatg ggaatcggaa
    17341 atacaatcat gggaacagtt gaaaataatg tgttatggtc attagcgctg atcttgctat
    17401 tgatgtcatt attctttaat atcgtgatcc gtatgattgg gaagaaagga gagctgaaat
    17461 aatgaacgca aaacgctcag ataaaattgc tacaggtgtt ttatatatag tttcggggat
    17521 catcgtattg attttagcag ctttactact ttatatctta gttcggggaa taccgcacat
    17581 ttcatggtcc ttcttgaccg aaccttccaa agcctaccaa gaaggcggtg gaatcgggat
    17641 ccagctattt aactcgattt atttgctttt gatcacgatg atcatcagtt ttcctatttc
    17701 tttaggtgcg ggtatctatc tttcagaata tgctggaaaa aactggctaa cagatgtgat
    17761 tcgcacgtcg attgaaatct tgagctcgtt gccttctgtc gttgtcggtt tattcggttt
    17821 cttggttttc gttattcaat tccagtatgg tttctcgatt atttctggtg cattggcttt
    17881 gactgtattc aacttgcctc ttttgactag aacagttgag gagtctttac aagcggtcca
    17941 ttacacacaa cgagaagcag gattggcctt aggtttgtca cgctgggaaa cagtgataaa
    18001 agtggtggtt ccagaagcga caccagggat tttgactggt gtgatcctaa gttctggaag
    18061 aatttttggt gaagcagctg cattgatcta tacggctgga caaagtgcac ctgcgttaga
    18121 ttttacgaac tggaatccat taagtgtttc aagcccgatc agcattttcc gtcaagctga
    18181 aacattagct gtccatattt ggaagatcaa tacagaaggc acgatgcctg atggtgtttc
    18241 tgtttcggct ggcgcttctg ccgtgttgat tattgctgtt ttactgttca acttcggcgc
    18301 tcgtgcgatt ggcaaacgat tataccgaaa aatgacttca gcctaaggag aagaagcaaa
    18361 atgaaaaaat ataactggaa tgaaacaaat gtcatcacca tgccaaaaga gaaagaagta
    18421 gcactatata cggatgatct acatgtctgg tatggcgaca atgaagcaat caaaggagtg
    18481 gatctggaat ttgaaaaaaa taagatcact gcactgattg gtccttccgg ctgcggaaaa
    18541 tctacgtatc ttcgttcgtt gaatcgaatg aatgatggga tcgctaatac gaaaatcact
    18601 gggaaaatca tgtatcaagg cgtagatatt aatgcgcctc aagtggatgt ttatgagatg
    18661 cgcaaacgga ttggcatggt ttttcaacgt cctaatccgt tcagcaagtc gatttatgac
    18721 aatattacat ttgctttgaa acagcacggc gaaaaaaaca aaaagaaatt agatgaaatc
    18781 gttgaaacaa gcttgaaaca agctgccttg tgggaccaag taaaagacag cttggacaaa
    18841 agtgccttgg ctttatctgg cgggcaacag caacgtttat gtattgctcg tgcgatcgcg
    18901 atgaaacctg atatcttgct gctagacgag ccggcaagtg ctttggaccc tatttcgaca
    18961 ggaacggttg aagaaacact tgtaaacttg aaagaagact atacgatcat catcgtgacg
    19021 cataacatgc aacaagcagc tcgtatcagc gactatactg cctttttcta tttaggaaaa
    19081 gtaatggaat atgacaaaac taacaagata tttaccagac caaaaattca ggcaaccgaa
    19141 gattatgtgt ccggtcattt tggttaagac ggaggcctca acatgacaaa agagattatt
    19201 tcatctaaag atcttcatct gtattatgga aagaaagaag cactaaaagg gatcgatcta
    19261 agcttcaatc aaggggaaat cactgcgatg atcggtcctt ctggttgcgg aaaatccact
    19321 tatctgcgtt ctttgaaccg gatgaatgac ctgattccga atgtaacgat caccggaagt
    19381 gttttatata aagacaaaga catctatggt ccaaaaacag atgttgttga actaagaaaa
    19441 gaaatcggca tggtattcca gcagccaaac ccatttcctt tttctatcta tgaaaatgtc
    19501 atctatggat tgaaattaaa aggggaaaaa gataaaaaag tccttgatca agtagttgaa
    19561 gaaagcctga aggcagcgtc tgtttgggat gatgtgaaag acaaattgca taaaagtgct
    19621 ttatctttat ccggtgggca gcagcagcgt gtatgtatcg cacgtgtttt ggcagttaat
    19681 ccggaaatca tcttattgga tgaaccaaca agtgcgcttg atcctgtttc tactgggaaa
    19741 atcgaaagta tgctgttaga attaagagag cggtatacga tgatcatcgt gacccataat
    19801 atgtctcaag cttcacgtat ttcagataag acggcattct ttttagatgg tcacttgatc
    19861 gaatacaatg atacgaagaa gatattcatg aatccagaaa aacaagagac agaagattat
    19921 atttcaggtc gatttggata aggaggaaac ggaatgctac gtacacaatt tgaagaagaa
    19981 ttgctgaatc tccataatca gttctacgaa atggggatga tggtgagcag cgccgtgcac
    20041 aagtcagttc gtgcatacat caaacacgat aaaatcttag cccaagaagt tatcgaaaac
    20101 gatataaata tcaataacat ggaaacacgc ttggagaaga aaagttttga aatgatcgca
    20161 ttgcaacagc cagttacaac tgatttgcgt atgatcatta cggtcatgaa agctagctcg
    20221 gatttagaac gtatggccga tcatgcggtg tctgttgcta aatccacgat tcgcgtaaaa
    20281 gggcagacga gaataccgga aatcgaaaaa gagatctccg atatgtccga ttatgtgaaa
    20341 aaaatggtgg acaacgtcct agttgcttat gtcaaaacag acgaaaaaga cgcacgtacg
    20401 atcgccaata tggatcaaag agtcaatgac tatttcaatc gaatctataa tgcaacaatc
    20461 aaaaatatgc aggaaaatcc tgagacagtc atcagtggaa cagactactt gaatgttgcc
    20521 agttatctag aacggatcgg agactatgtg acaaacatct gtgaatggat cgtctacttg
    20581 gctacaggga aaatcactga gttaaatacg aaccacaatg agattttcta aactgaagct
    20641 gtgattatgc cgtttgttta ggaaaagagg ttgtgaaatc agcggttgtg aaaaagctgt
    20701 tctgcatcaa gtaataagaa cagtcaaaca aaaaatagct cctcatattt ttcgtttggc
    20761 tggacttatt accgcagatg caagcttttg aacaccgttt attcgggaaa gggtttgtga
    20821 caaaacatgt cacaaaccct tttcatcatt tcatagaaag gataaagaat gcaaa
//
LOCUS       DARBEC010000051        20485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.051, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000051 DARBEC010000000
VERSION     DARBEC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.051"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            4..552
                     /gene="hslV"
                     /locus_tag="SCL80_001720"
     CDS             4..552
                     /gene="hslV"
                     /locus_tag="SCL80_001720"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEG1107588.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            566..1966
                     /gene="hslU"
                     /locus_tag="SCL80_001721"
     CDS             566..1966
                     /gene="hslU"
                     /locus_tag="SCL80_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEG1107589.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            1984..2775
                     /gene="codY"
                     /locus_tag="SCL80_001722"
     CDS             1984..2775
                     /gene="codY"
                     /locus_tag="SCL80_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEG1107590.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            2857..3732
                     /locus_tag="SCL80_001723"
     CDS             2857..3732
                     /locus_tag="SCL80_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEG1107591.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCL80_001724"
     CDS             complement(3864..4502)
                     /gene="plsY"
                     /locus_tag="SCL80_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEG1107592.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            4629..5063
                     /locus_tag="SCL80_001725"
     CDS             4629..5063
                     /locus_tag="SCL80_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEG1107593.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            5229..7274
                     /gene="parE"
                     /locus_tag="SCL80_001726"
     CDS             5229..7274
                     /gene="parE"
                     /locus_tag="SCL80_001726"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEG1107594.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            7286..9736
                     /gene="parC"
                     /locus_tag="SCL80_001727"
     CDS             7286..9736
                     /gene="parC"
                     /locus_tag="SCL80_001727"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEG1107595.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            10013..12244
                     /gene="pflB"
                     /locus_tag="SCL80_001728"
     CDS             10013..12244
                     /gene="pflB"
                     /locus_tag="SCL80_001728"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEG1107596.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            12488..13249
                     /gene="pflA"
                     /locus_tag="SCL80_001729"
     CDS             12488..13249
                     /gene="pflA"
                     /locus_tag="SCL80_001729"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEG1107597.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            13334..14260
                     /locus_tag="SCL80_001730"
     CDS             13334..14260
                     /locus_tag="SCL80_001730"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEG1107598.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            14442..15335
                     /locus_tag="SCL80_001731"
     CDS             14442..15335
                     /locus_tag="SCL80_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEG1107599.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            15469..15594
                     /locus_tag="SCL80_001732"
     CDS             15469..15594
                     /locus_tag="SCL80_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1107600.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(15728..16360)
                     /locus_tag="SCL80_001733"
     CDS             complement(15728..16360)
                     /locus_tag="SCL80_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107601.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(16508..17050)
                     /locus_tag="SCL80_001734"
     CDS             complement(16508..17050)
                     /locus_tag="SCL80_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEG1107602.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            17185..17574
                     /locus_tag="SCL80_001735"
     CDS             17185..17574
                     /locus_tag="SCL80_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107603.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            17578..18342
                     /locus_tag="SCL80_001736"
     CDS             17578..18342
                     /locus_tag="SCL80_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1107604.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            18471..19418
                     /locus_tag="SCL80_001737"
     CDS             18471..19418
                     /locus_tag="SCL80_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107605.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            19548..20288
                     /locus_tag="SCL80_001738"
     CDS             19548..20288
                     /locus_tag="SCL80_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107606.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
BASE COUNT         7193 a         3481 c         4316 g         5495 t
ORIGIN      
        1 aacatggtcg aatcaccatt tcattcaacc actatctgtg ccgtcgaaaa agacgggaaa
       61 tttgctatgg ctggagatgg ccaagtaaca atgggtgaat ctgttgtcat gaaaggcact
      121 gcaaagaaag tacgacgtat ctacaatgga gaagttgttg taggtttcgc cggaagtgtt
      181 gcagatgcgt ttactttaga agaaaaattt gaaggcaaac taaacgaata taacgggaat
      241 ctacaacgcg cagctgtcga attagctcaa gaatggcgga cacagcaatc tatgcaaaaa
      301 cttgaagcga tgctgatcgt aatgaatgat aaagaaatgc ttcttgtttc aggtacagga
      361 gaagttatca caccagatga tggtatctta gctatcggat caggagggaa ttttgcgttg
      421 gctgcagccc gagcaatgaa aaactatgct caaaacgaca tgagtgcgaa agaaattgca
      481 gaaaatgcgt taaatattgc cgcagatatc tgtatcttta ctaatcataa tattatcgta
      541 gaagaactgt aagaggtgag tagatatgta tgaattaaaa caaacaccaa gacaaatcgt
      601 aagtgaatta gatgaatata tcgtaggaca gcacgcagca aaaaaatctg ttgccgttgc
      661 tttgcgtaac cgctatcgcc gtttgcagtt agatgaaaaa atgcaacaag atattacacc
      721 aaaaaatatg ctgatgatcg gacctacagg ggtcggtaaa acagaaatcg cacgacgttt
      781 ggcaaaaatc gtcaatgcac catttgtcaa agtcgaagca acaaaattta ctgaagtcgg
      841 ttatgtcgga agagatgtag aatcaatggt tcgggattta gtagaaaatg cgatccaaat
      901 cgtagaaaaa gagcagtaca gccgtgtata cacaagtgct aaaaaaaatg cagaaaaacg
      961 tttggtaaaa atcttggtac ctggtatcaa aaaagaacag aaacaatcca ctaatcaatt
     1021 tgaacaaatg atgaatcttt ttaatgctag tcagcaaaat accgctgaac cgcaagaaga
     1081 agtgacagaa gatattcgaa tcaatcgccg tacagtcttg gaacaactac aaaagggttt
     1141 actggatagt cgtgaagtaa ccattgaagt tgaagaccaa aagaaacctg caccaatgat
     1201 gaacaatgga ttggagcaaa tggggatcga cctaaatgaa actctaggtg ctttgacacc
     1261 gaagaaaaaa gtggaacgga cggtaacagt caaagaagct ttagagttat tgatcaagga
     1321 agaatcagca aaattagtca aggatgctga tatccatagt gaggcaatca aattagcgga
     1381 agaaagtgga attattttta tcgatgaaat cgataagatc acttctaaat cacagcaatc
     1441 tggagaagta tccagagaag gcgtccaaag agacatattg ccaattgtcg aaggttcaca
     1501 agtaaacaca aaatacggga tcatccaaac agatcacgtc ctattcattg cttcaggagc
     1561 tttccatttg tctaaaccaa gtgatctaat tccagaactt caaggacgct tcccgatacg
     1621 ggtagaatta gatgatttga cagctgaaga ttttgtgaag attctgaccg aaccaaataa
     1681 tgctttagta aaacaatacg tcgcgttgat cggtacagaa aatgtgtcag ttacgtttac
     1741 aaaagaagca attgaacgga tcgctcaaat cgctttcgat gtcaaccgtg acacagataa
     1801 tatcggcgca cgcagactgc ataccatttt ggaaaaacta ctagaagatc tattatttga
     1861 agcaccagat atgcaaatgg gagaaataac gatcacagaa gcatatgtgg atgacaaact
     1921 tggctcaatt gcggcaaatg aagatttaag tcgctatatt ttataaaaaa tcggaggacg
     1981 acaatgacag acttgctaac gaaaacccgc aagataaaca cacttttgca gcaaaagaat
     2041 acttttgaca tgcaagcaga tttgccttac gataaaatgg ctgtcacact aggtggtatt
     2101 cttgacagca acacctatat cataggaagt gacggagtat tactaggtta taatgaacgt
     2161 cacgatgtga acaatgaacg aatcaaaaca atgtttgttg agaaacaatt tcctgaatct
     2221 tatactgata cagtggcaca gttgatgaaa actgaagcta atatcccaat tactagtgat
     2281 attactgcat ttccggtaga gtcaagagag aaatatccat ttggtctgac gactgtcgtt
     2341 ccgatctttg gagctggcga acgtttgggt acgattatct tatctcgtat ggaacaatcg
     2401 tttgcagatg acgatctagt cttagcagaa tatggagcta cggttgtcgg tatgcagatc
     2461 ctttatcaga agtctcgaag catcgaagca gatgtacgga gtgcaacggc tgtccaaatg
     2521 gcaatcaaca cattatctta tagcgaattg aaggctgtac aagctatctt tgaagcattg
     2581 gatggagatg aaggtcgtct gactgcttct aatattgccg atagtatcgg catcactcgt
     2641 tctgtgatcg tcaatgcttt acgaaaattg gaatcagctg gaatcattga atctcgttcg
     2701 ttaggcatga agggaactta tttaaaagta ttgaattccc gttttaaaga cgagttagca
     2761 aaacacagtt attagaagta aggggactgg ggcagataga aatgtttgtt ccagccttcc
     2821 cattttaaaa agtaagtcga agttggagga atggcaatga ctgttattat tcaaaatgac
     2881 cagctgatcg ccgaaatcgc tgaacacggt gcggaattga tcagtctgaa atcaaaagaa
     2941 catggtttag agtatatctg gcaaggtgat ccagcgtatt ggggcagaca tgcaccggtt
     3001 ttattccctt ttgttggacg tttaaaagat gatcagtatg tttatcaagg gaaaacatat
     3061 caaatggggc aacacggatt tgctcgggat atggattttg aagtcattga acatggacaa
     3121 gaaatggcta gtttcatctt aaggagtacg ccggagacat tagaaaagta tccatttgat
     3181 tttgaattag tgatcagcta tgaattagga ggagacggca tcacggttca ttatcaagtg
     3241 gcaaataccg gtgaaaaaga aatgttcttt tctatcggtg gacatcctgc tttcaatgta
     3301 cctcttgaag aaggattgtc atttgaagat ttctatctgg cattttctcc attgaaatca
     3361 agattacgcc taccattgaa aggcccattc atcgatatgg atcaaaagac gttaggtcag
     3421 acaaatacga atctggcttt gaatcgtgaa ctttttgagc aagatgcctt ggtttttgaa
     3481 acaaggggtc taaatgcgta cgccattcga agtgaaaaat cttcccatag cgtaacttta
     3541 agctataaag atatgcctta tgtagggatc tggtctcctt atccaaaaga agcgcctttc
     3601 gtttgtatcg aaccgtggtg cggaatcgct gacacggtga acagtagtgg aaacttgatg
     3661 gaaaaagtag ggatcaatca actaggtgcc catgaaatct tcaagacaaa atattccatc
     3721 acagtgaagt aagaggttgt gaccgagaag ttagcaggga acaccgttta ccaaggtgtg
     3781 taaaaaagct atcctgtaag taataaggga gtgtgacatc gatttgtcac actcccgttt
     3841 tatctctttg gaatgaaaaa gtatcaggat tttttcttgc tgctattcaa accaaacggg
     3901 attcgacttt cagtatggtt tctgatacgc ttgatattgt ctttatgccg ataaaagata
     3961 aaggaagaaa ctaaaaaggc aatgatcgtc agtaaccaat tgggcttagc caaaatcaca
     4021 ggagctacat aaggaaggac gatcgtagat aaagtgatca atactgcact gatcatactg
     4081 ctcaaactca ccatacttgt acaaaataaa ctaacgataa agatgataca agaataaaca
     4141 aaaaatacag gattaaatcc taaaagcatt cctgcacttg tcgcaacagc ttttcctcct
     4201 ttgaacccag caaaaatcgg aaatgtatgg ccaagaatgg cgcatacccc aaaccaaaga
     4261 gggttaatcc cagaaaggtg gaagatcatc ggcaaacaag tagccaatgt accttttaaa
     4321 atatccataa gcaggacaac aatcccggct gttttgccta atacacgaaa tgtattggtc
     4381 gttccagtat taccgctccc gtgttgtcgg atatcttttt tgaaaaacaa tttgccaatc
     4441 cagacaccgg aaggaataga acctaaaaga tatgctaaaa taagtaataa gatcaatttc
     4501 attttttacc ctctttcaag ttgaaaacta tcctttattt tagcatgaat aggtaacagg
     4561 aacaattgaa aaaatgaaag atacaagtat aatagatgga ataaaaagaa aagcaagggg
     4621 tggcagaaat gtctgtaaaa aatccaacac aagaaacgat tttcaactat ttggaagaag
     4681 caaaaaatat tgccgtcgta ggcttgagca ataaaccaga acgaacaagt tacatggttg
     4741 cacaggtatt gcaagacaat ggatataaaa tcataccagt aaatccatta ttggctggtg
     4801 aagaaattct aggcgagaaa gtctatgcga atttgacaga aatcgatgat ccaatcgata
     4861 tcgtagatgt atttagacgg agtgaatttt tacctgaaat agcaggtgat tttttgaaaa
     4921 ctgatgctaa agtgttctgg gcacaattag ggattgaaaa caaagaagca gctgagatgc
     4981 taagaaaagc aggacgtaca gatatcgtaa tggatcgttg tattaagatt gaacttggta
     5041 agttgaaaga aaaccaaaaa taaaaatgac cacaaagaag agatgatcga tagaggggaa
     5101 tgtcgaaaaa gcggttagga gataattaaa attgatttct cgtctaccct cttttttttc
     5161 acgacctttt ctagtatgat aatcgtggtg cggttttgcc gcaaagtatt attgaaggag
     5221 cgtttgcctt ggcaaaaaaa gtaaacaacg aatacaatga ctcgtccatc caagtattgg
     5281 aaggattaga ggcagttaga aaaagaccag ggatgtacat tggatccact gacagccgag
     5341 gcttgcatca tttagtctat gaaatcgtcg ataatgcagt tgacgaagca ttatcaggtt
     5401 atggcagtga gattgatgtg accatccatg aagacaatag tataactgtt gcagatagcg
     5461 gacgtgggat gcctgtagga atgcatgctt ccggaatacc aacagttgaa gtgattttta
     5521 ctgtgctgca tgctggcgga aagtttggtc aaggagggta caaaacttct ggtggactgc
     5581 acggtgtagg agccagtgtg gtaaatgcct tatcgaaatg gctaattgta acgatcgtta
     5641 gagatggcgt agagtatcag cagaaattta aaaacggcgg taaacctgac ggtactttga
     5701 aaaagatagg aaaaacaaaa aaagcaaacg ggacgactgt ccatttttta ccagatgata
     5761 caatattcag tacaacgaaa ttttcatatg aaattttagc cgaacggtta agagaatctg
     5821 cctttcttct aaaaggcgtt aaaatctctc tttctgatct tcgtggagaa gaaccagtca
     5881 aggaaatttt tcattacgaa gaaggaatca aagaatttgt tgattatctg aatgaagaaa
     5941 aagatacatt gacaccagtt gtttattttt caggagaaaa agaaggtatc gaagtagagg
     6001 tcgcttatca atacaatgac ggttattcag aaaatgtttt atcttttgtc aataacgttc
     6061 ggacaaaaga tgggggaacc catgaagctg gtatgaaagc ggcgatgacg aaatcttata
     6121 atgaatatgc gagaaaagtt ggtttgctga aagaacggga caagaatctg gaaggtagtg
     6181 atttcagaga agggttagct gctgtacttt ctatccgtgt acctgaaaat cttctgcaat
     6241 ttgaagggca aacaaaagaa aaacttggta cgccagttgc tcgtaccgta gtagataacg
     6301 tcatcagtga acaaatgggc ttttatttgc aggaaaatag cgaaatgagc cagatgttgg
     6361 tccgtaaagc aatcaaagcc agagaagcga gagaagcagc acgtaaagcc agagaagaaa
     6421 gccgaaatgg gaagaagcgt aaaaaaggcg aatctcttct ttcaggaaaa ttgacacccg
     6481 cacaatcccg aaaccctaag aaaaatgaat tgtatttagt cgaaggggat tctgctggcg
     6541 gatctgctaa acaaggaaga gacagaaagt tccaagcaat tctaccttta cgaggaaaag
     6601 tcatcaatac cgaaaaagct aaaatgcaag atatcttgaa aaatgaagaa atcaatacga
     6661 tgatctatac gattggtgca ggagtcggtc ctgagttttc tatcgaagac tgtaattacg
     6721 acaaagtaat catcatgacc gatgcggata cagacggcgc ccatattcaa gtattactgc
     6781 ttactttctt ttatcgttat atgaaaccgc tgattgaagc tgggaaagta tatatcgcgt
     6841 taccgccttt atataaagta tccaaaggac aaggaaagaa gcaagtcatt gaatatgcgt
     6901 ggacagatga cgaattggca gcaatgatta aaaaagtcgg caaaggatat atgctgcagc
     6961 gatataaagg acttggagag atgaatgcgg aacaattgtg ggaaacaacg atggatccga
     7021 cttcccgtac gttgatccgc gtccgtatcg atgatgctgc gcaagctgaa cgtcgagtaa
     7081 ccacactgat gggtgacaaa gtagaacctc gaagaaaatg gatcgaaaat cacgtgcaat
     7141 ttacgctcga agaagatggc agtatcttag ataaaaaaga ggatactgaa atctctcctt
     7201 cagtttctaa tgatttatta gatgaagaac gagcagataa aaatgaaaac aatcagttat
     7261 ttgaagtaga ataggaggtc acctgatgga aaatcgacaa gaagtacaag aattaacttt
     7321 agaagaagtg atgggtgacc gcttcggacg ttattccaaa tatattatcc aagaacgtgc
     7381 actgcctgat atccgtgatg gattgaagcc agtgcaacgc cggatattat ttgccatgaa
     7441 caaagatggc aatacgtatg acaaaggatt ccgtaaatca gcaaaatctg tcggaaatat
     7501 catggggaat tatcatcctc atggtgacat cagtatttat gaagcgatgg tccgcatgag
     7561 ccaagactgg aagttgcggg aagtactgat cgaaatgcac gggaataacg ggagtatgga
     7621 cggtgatcca cctgctgcga tgcgttatac tgaagccaga ttatccaagt taagtggaga
     7681 gatgctagca gatatcgaga aagaaacagt cgatctcgtg tggaattttg atgatacaga
     7741 aaaagaacca acagtccttc cagctcgata cccaaacctt ctcgtcaatg gatcgacagg
     7801 tatatctgct ggatatgcaa cagaaatccc gactcataac ttagctgaag tgatcgatgg
     7861 cacaatctat atgatcgatc atccacaagc gagccttgaa aaattgatgg aatatattcc
     7921 tggacctgac tttccaacag gagggatctt gcaaggaaaa gatgaaatca aaaaagctta
     7981 tgaaacagga cgaggaaaag ttattcttcg ttcgaaaaca aagatagagg caatgaaggg
     8041 taataaacag caaatcgtga tcacagaaat cccatatgaa gtaaataaag cgaccttggt
     8101 gaaaaaaatg gatgagatcc gtttgaataa gaaaatcgat ggaatagctg aagtacgaga
     8161 tgaaagtgat agaacaggtc tgcaaattgt cgtcgaattg aaaaaagacg ccaatgctca
     8221 aggaattttg aattatttgt tcaaaaacac tgagctacag atcaattaca atttcaatat
     8281 ggtcgctatc gaccatatga ctccgcatca agttggactg aaagacattt taagaagtta
     8341 tatcgagcat cgaaagcaag tcataacaaa acgtagtcaa ttcgatttag ccaaagctca
     8401 aaaaagacag catatcgtag aaggattgat gaaagcttta tcgattctgg atgaagtaat
     8461 cgcaacgatt cgtgaaagta aagacaaaaa agacgccaaa aagaatttag tcgacgtctt
     8521 tcaatttaca gaagaacaag cagaagcaat tgtcacattg caattatacc gtttgacgaa
     8581 cacggatatc acagagcttc aaaaggaatc cgaaagcttg attgcgcaaa tcacagagtt
     8641 gaataaaatc ctttcaaatg acaaagagct tttctctgtt atgaaaaaag aattgcgaga
     8701 agtgaaaaaa aattattctt ccgctcgtct aacgacaatc gaagacgaaa tcgaagaaat
     8761 caaaattgat acgcaagtct tagtggcaca agaagacgta attgtcagtg tgacgcgaga
     8821 aggctatgtg aagcgaacaa gccttcgttc ttatagtgct tccaaaccag aagaaatcgg
     8881 gatgcgagaa ggcgactact tgctatactc aggtgaattg agtacattgg accatgttct
     8941 tctgatcaca aataaagcaa atgtgatcta ccgccctgtc catgaactac ccgatttgaa
     9001 atggaaagat gctggtgagc acatttctca aacaatctcc aatctgtctg tagacgaatc
     9061 gattttagct gttttcccat atcaaaaaat tcaagcagag aaaacatttg tattcatcag
     9121 caagaatgga ttgatcaagc aaacacgaat gacagagttc gaaccatggc gcacctataa
     9181 gagccgtccg ctcagctgta tgaagctaaa ggcggaagaa gatgaactga ttgctgtgta
     9241 tctgaaagaa gatcagccag gcttagatgt tttcttagta acgcatcaag gaatgggttt
     9301 gcgctatcct ttaacagatg ttcccgttgt tggagcaaag gcagctggag tgaagtctat
     9361 gaatttgaaa gatggagata ggattatcaa cgggctgctt gtgttagcag aaggtgatac
     9421 gccgatcgta atcgttactc agcgaggagc tgttaaacgc atgcttgctc aagaaatcag
     9481 ccaaacttca cgtgcgaagc ggggagtgac ggtcatgcgt gagctgaaaa aaaatccgca
     9541 ccgtattatc tatatgtcgg aaggaagatc aaaagaaata accttgatga atcaaaaagg
     9601 gcagcagtta acgattgacc caactgattt ccctataggt gatcgaacat cgaatggatc
     9661 atttgcgatg gatgagaaaa aaggtggaga agtcatcgaa gttatcgatg ctcctgagat
     9721 tttcatcgag tcgtaactgt tgtagtattt taatgaagag acgccatctg tattaataca
     9781 ggtggcgtct ctttttcatt catttttaat aaatccttat acgtttgcaa aaaaagatga
     9841 aagtgttcac aatgcttaaa tgttggtatg tcaacgttga tttaaatata tgtaaataaa
     9901 acataaaaat tgttaaataa aaaacaagaa aacccttgca aagatagggt gctgtgttat
     9961 actaatcacg taaaaactgt tacataaaac agaaatttag gaggtccata caatggagca
    10021 atggaatgga tttaaaggta ccaaatggaa agaaggcgta gacactagag actttattca
    10081 aaataactac actgaatatc gcggtgatga cagctttttg gaaggcccaa cagaagcaac
    10141 agaaaaatta tgggatgaat tgcaaaagtt aaatgatatc caattcgagc gtaacggtgt
    10201 ctatgatatg gacacagaaa tcgtatcaac tattacttca cacgatgcag gctatttgga
    10261 taaagatttg gaaaaaatcg tcggtctaca gacagacaaa cctttgaaac aagcattcat
    10321 gccgttcggc ggaatcaata tggctaacaa tgcgctaaaa agcaatggct atacaccaaa
    10381 cgatgaattg acacatattt tcactgactg gcgcaaaaca cataatcaag gtgtatttga
    10441 cgcttatact gctgaaatgc gtgctgctcg taaaaacaaa atcattacag ggcttccaga
    10501 cgcttacggt cgcggacgta tcatcggtga ctatcgtcgt gtggcattgt atggaatcga
    10561 ctatttgatg gaacaaaaga aaaaagatca tgatatgact ggttatcata caatgagtga
    10621 agaagtgatt cgtttacgtg aagaaatcac tgaacaatat cgtgcattag cgcaaatgaa
    10681 agaaatggct gcgaaatatg gctatgatat ttctaaacca gctgcaaatg caaaagaagc
    10741 tgtccaatgg ctatacttcg gatatttagc agcaatcaaa tcacaaaatg gtgctgcgat
    10801 gtcaatcgga cgtatctcag cattccttga tatctatatc caacgtgatt tggataatgg
    10861 agtaatcaca gaaaaagaag cacaagaatt gatcgaccac ttagtaatga aattacgtat
    10921 ggtcaaattc gcgcgtacac ctgaatataa ccaattattc tcaggattcc ctatctgggc
    10981 tactttatcc attgcaggta tgggattaga tggtcgttca ttagttacaa agaatgattt
    11041 ccgtctgctt catacattaa caaatatggg accttcacca gaaccaaact tgactgtttt
    11101 gtattctgaa caccttccag aaggattcag aacatatgct agcagaatcg ctatcgaaag
    11161 ttcttcgatt caattcgaaa atgataatca gcttcgtgaa caatggggtt ctgatgattg
    11221 tgcgattgct tgctgtgtat caggaacagt tgtcggaaaa gacatgcaat tctttggcgc
    11281 tcgtgctaac ttggctaaaa cactattata tgccatcaat ggcggagtcg atgaagtgac
    11341 tggcgcccaa gttggaccag aatatcgtcc aatcacatca gaaatactag actttgaaga
    11401 agtgaaacat aacttcatga atatgatgga ttggttagca gaactatatg tcaatacgtt
    11461 gaacatcatt cattatatgc atgataaata ttcttatgaa gcagctcagc tggctttgat
    11521 ggattctgac ttgcaacgca catttgctac aggtattgct gggatctctc atgcggcaga
    11581 cagcttgtca gcgatcaaat atgcaaaagt aaaagcaatc cgtaatgaaa aaggaatagc
    11641 agttgatttt gaagtggatg gcgacttccc taaatacgga aatgatgatg accgtgcaga
    11701 tgaattagtt tcatggatct tggaatactt catgggacaa atcaaacgtc ataaaactta
    11761 ccgcaactca aaaccaacaa cttcattatt gacgattact tcaaatgttg tttacggtaa
    11821 aaatacagga aatactcctg atggccgtga agctggaaaa ccacttgcgc caggagcaaa
    11881 tccaagttac aatgcggaac aaagtgggtt attagctagc ctgaactcaa cagctaaaat
    11941 gaattacggc tgtgcattgg atggaatttc aaatacccaa acaatcaatc cttcaggtct
    12001 tggaaaagaa caagatacaa gaatcgaaaa cttgcgtaat gtcttagatg gatacttctc
    12061 aaaaggagct tatcatttga atgtcaatgt attcagtaat gatttattgc gtgatgcaat
    12121 ggaaaatcca atgaaatatc cgaacttgac gatccgggta tctggttatg cagttaaatt
    12181 ccgtgacttg acaagagaac aacaattaga tgtcttgatg agaacttcac acgacagaat
    12241 gtaaaaagtt cgtgaaaaaa gtttttcgac ttagcgtcga aaggacgttg aatagccatc
    12301 gcatcaaaat cgaataatca gtatgcttat ttgaaagaaa cgatttacag gggcaggact
    12361 tgtatgttcc gtccctgtaa attttacaca taacgaagcg gcaattgcga aaaattttgt
    12421 tcagaaagct tcgcttcgac agctaacttg tacttaaaat gaaatcaaag aaaagatgtg
    12481 aacttaaatg gaagaaaaaa caatcggcta tgttcattct atcgaaactt tcggttcagt
    12541 agacggacct ggactgcgct ttgtcgtgtt tatgcaagga tgccgcatgc gttgccaatt
    12601 ttgtcacaat ccagatactt ggaatattgg cggagggaaa gaatacacag cagatgaact
    12661 tttagataaa gcagaacgct tcagacctta ctggggagat aaaggtggta ttactgttag
    12721 tggtggagaa cctctattgc agatcgattt tttgatcgaa ctatttaaaa aagcaaaaga
    12781 gcggaagatg catactacat tggatacatg cggaaaacct tttacgtatg aagaaccttt
    12841 cttttcacgt tttcaagaat tgatgaagta tacagattta ctgttatttg acatcaaaca
    12901 catcgataat gaagagcata aaaaactaac gcattgggac aatgaaaata ttttagaaat
    12961 ggctcaatat ttgtcaaaaa tcaacaaacc ggtttggatt cgccatgtac tagttcctga
    13021 gcgaagtgat tatgatgaat atttgatccg actagacaac tttattcaaa ccttgtcaaa
    13081 tgtagacaga gtagagattc ttccttatca tacgatggga aaatacaagt gggaaacact
    13141 tggtttgaaa tatccattag aagggatcga accacctaca aaagaacgtg tcgaaaatgc
    13201 taaacgattg cttcatacag aagagtatac aaaatattta acgcgatagg tcgcatcgga
    13261 aagaagataa gtattgcttc tttccgtttt tttgctatta ttaaatagga gaaatcacaa
    13321 aggagcgaat gacatgtcaa aaattttagt ttttggtcac caaaatccag atacagatgc
    13381 aatcggggca gccattgcgt tttcacactt gcaaaatgaa ttaggtaaag aaacagaagc
    13441 agttgctcta ggtacaccta atgaagaaac acaatacgca ttagaccact ttcagctatc
    13501 tgcaccacga gtagtagagt cagctaaagc agaaaatgca gatgtcatgc ttgtggatca
    13561 taatgaattc caacaaagta tttcagatat cagcgaagta aatattttag ctgtagtaga
    13621 ccaccatcgt attgcaaact ttgaaacagc taatccattg tactatcgtg cagaaccagt
    13681 tggatgtaca agtacgatta ttttaaaact atataaagaa aaccaagtaa caattcctaa
    13741 agaaatcgca ggaatcatgc tctcagctat catttcagat acattgttgt tcaaatcacc
    13801 aacatgtacg cctgaagatg aagcagctgc aaaagaactt gcagcaatcg ctgaagtcga
    13861 tctaaacact tatggacttg aaatgttgaa agcaggaaca aatctaagtg ataaatcagc
    13921 tgcggttctt cttgatttgg atgcaaaaag tttcccaatg ggcagttcca acgttcgtat
    13981 cgctcaagtc aatacagtgg accttaatga agtgatggat cgacaagcag agctagaagc
    14041 agcaatggaa gatgaaatga aagcaaatgg ctacgatctg ttcgtattag ttgtcacaaa
    14101 catcttagac agtgattcag aattacttgt tgctggtaaa ggaaaagaaa aagtagaaga
    14161 agctttccaa acaactttga ccaacaaccg tgctttgctt aaaggtgtcg tttcacgtaa
    14221 aaaacaagtc gtacctcaat tgacggctgt atttagctaa tcaaatggta gtatgaagca
    14281 ttcaaaatga atggataagc gattgatata aaaagaaaca tagaacatac taaaggccga
    14341 agaaatggag gagaataaca gtcaattatg actcttgttc ttcctccttt ttacgtaaaa
    14401 gccttttaag taaaataaaa cggatagaaa agagaacata tatgaactat tattcaaaaa
    14461 ataaggaaaa cttttatcaa aaactgaccg atgatccgct tttttcttca ttgaccgatt
    14521 atctatatga acatcgagaa caagaaacga ttctaagaga attgaaaaaa gaattttcgc
    14581 aaaataaatt ttcccatttt cttgatttat tgatcgatgc aggattaatc aagagggaag
    14641 aacgaagata tcatttgaac tttccaatat ttgatcctaa agattacttg caacaagcca
    14701 cttcggctgc tgaaaccata gctgaacaat tgaagagact ctctgttgat gaacaaaaat
    14761 tagcgatggg agaagttatt tgggcgtatt gtttcgaaga tgaacggaaa gaagcgtatt
    14821 tttatggagt aagaaacagc cgagagacag aattgctgag gacaactgca ggaaatgaga
    14881 agtaccgttt tatcacactt tcctctatag aacacttccc gcttactttg gctaattact
    14941 tttttgtaca gaagaatcag cttcctgtaa cgaaggcatt caaagaatta gccgaactta
    15001 ttggagacgt aaatgaatcg tacttttttg atcaaataga agttatcgtt gacaggatac
    15061 gtaagaataa atataaaaat cgccggccta gtattttcca tcagtctcta cttgtgacga
    15121 atactataaa ggaagaagaa tcattcacat tagaattacc tattgtcgaa aaaaacaatt
    15181 ttgagattga attaccaaca ttggatcctt cattgacaat ggaggaaatg gcctttctta
    15241 aacgacaaat cttttctgag ctgagcaaga aatttatacc gcatgcattt tcttatatca
    15301 aagaatatgg aacagtttta gtgagtaaaa cctaagagag cagtaagcaa aaattatact
    15361 gactcttgca ggaggagcct ttctcttcct ttatggttaa aagaattatt tgagtatttt
    15421 gcctatctac cacctattag attcactaca tggtctggaa acaaaggaat gcaaaaaaat
    15481 ggtgagaaat gtggaatgac aaaggaagta gtgataagaa aagttagatt tttaaataat
    15541 caatattatg attcagtcaa gtatggcatt ttatgggaag aactagctga ttagattagg
    15601 aagtccattt gataatccag aatcactaag aaacgaatgg cgagtcatta ataatttatc
    15661 caatcttggg ttattctttt agattgaagt gtttaaaact aaaaaagcat ttatgaagtc
    15721 gtaatagtca ggcttcaaaa tgctttttat tttgtttgtt ttttctttgc cacataaata
    15781 cacctagact gacgatgaag agcatatcca acagatttcc tgcgacttgg atcgtcgaag
    15841 ggttggtcag tggcattaaa gcaaggacaa aaaaggaaaa caagctgtaa ataaaacagg
    15901 tgacaggaac taatcgaaag aagagcggac atttcaattg gctaaaaatg agtgaaaaaa
    15961 taaaataagt aaaaatggat aaaatcacga ctaaaccatt catgataaag aagaaaagcg
    16021 tattgtgatt aaacgaataa aaaagaattg ctaaaaaaga aaacgtttta aatgcaatca
    16081 atacgcttga agcttcgttt aacgtaccag gatgtcgatg aaaaaaatag gcaccgaaat
    16141 aaacagaaaa cagtaagccg caaagagaag acaaaatcaa aaaaaacgga ttttcttcgg
    16201 cttctgtgat atcagaatga tccaaaagca accagcctaa tgcactagaa ggcgcatgtg
    16261 aggtcttaag gtatgaataa catagaaaaa ataagatcgt caaagttgca aaaaaagagc
    16321 ctaaatgtaa aaaattagtg gttctacgtt gaatttccat aatatctcca aattaaattg
    16381 ataagtatat aaagtataat aacaaattga tgagtatatg tcgatgtttt ttaatcaaaa
    16441 taataaataa aaactgtcta acatttaaaa tttaatttag atattagaca gtttttaagt
    16501 ttgtgagtta ttttccttgt cgttttctca cttttttagt ccagaaacca cctgaaatat
    16561 attttggctc ataagaaatg ataaaagcac gctcttcgat ttctttgatc aggcgataca
    16621 gttcacgttc gctttttcta ggagaaagta tttcgagaac tagccgctca ccttccagcc
    16681 ccattgcaga agtctgtgtc acaccataac cgtgttctct cagtataccc ggcaaacttt
    16741 tatcttctgc gctatttgct ggcaaaatcg ttgtgaccat gatgtatcct aatgccaatt
    16801 tgtcttcaat caaaattccg acactgatac ctactgcgta acctaacgca tagacaaata
    16861 aattcagtgg attatctaaa cgatctaaaa caagggataa gccgacgata taaatcgtga
    16921 tctcgaccat actgacaagt ggagcaatca gtcgatagcc tttcattgtc aacatgaaac
    16981 gtaacgtgtt cagagtgata taagcaaaat taatgaggaa gatcatcccc aacatttttg
    17041 gatcaatcat ttgtaaaact cctttcattt atataacaac aatatcaaaa aaaatcgtaa
    17101 gtgaagaaaa attgaaactt tatacataca aaaaaatgat aaaatgatta taatagaatt
    17161 ataaagatag gttaggtgat taaaatgaaa acggtcaatc gtgtgttttg tgccagccac
    17221 gtgctaagtc tccttctcag tatatactta ggtgtctatt cgattcaaca gccaattgag
    17281 gtagagaaca ttgtttttca gatgtcgctg acagatggtt taattttaag cgtttttttc
    17341 tttttgatac tgtttattgg taatattttc ggtagcgttg ccagtttttt gaatttttct
    17401 atttatccac tcctttcgct agcattagga gtggtgggac tttgttctct gtgcttattt
    17461 cccgagccat tcagtcctgc atttatatta tttggtacac tgaacctgtt ccagacaact
    17521 gttggagcat ggttgctatg gcgatcagga aatttaatga aaataggtga gtaacaattg
    17581 aatccaatta tattgaaacg ttctgtccat catattccag tgttagaagt tgttctgaaa
    17641 gaattaagag aggaacgtat tcccgttgtc atctattatc acggatggca gacaagtaag
    17701 gagttagttt tgacgcaagg aagaaaaatt gcgcagcagg gcattcgtgt gatcctgcca
    17761 gatgcgatga atcatgggga gcgcaaacag ccggtttctt ctattccatc ctatacattt
    17821 tgggatagta tctacggaaa tctgtttgaa tttgacacac ttatcaagca tttagaaaaa
    17881 agaggtcttc tacatgatca gcttgcagtt ggcggggttt cgatgggggg aatgacaaca
    17941 tgcgccttgt tggctaaaca tcctcatata gtagcgggcg tgtgtttgat gggttcccct
    18001 gcacctttgg aatatggaaa agaaatcgta aggcgcgcat cggaatataa ttatgtcgta
    18061 ccaaaagact attttgattt gatttcatgg gttaagagct atgatttgtc attgcagccg
    18121 gaaaagttag ctggacgtcc tctgttcttt tggcatggac aagctgacga aaaaatccct
    18181 tatatacaaa cggaacgatt tgtagaaaaa aatcggcata agtcttacgg aaaagaaatt
    18241 gtttttcgtt cctcaaaaac acagcgacat atggtacaaa taccactaat ggaagaagct
    18301 tccacttttt tagcaaaaaa tttgataaaa aagtataaat aattgaaacg gaaaatcact
    18361 catgacgata gaggattttc cgttttttat tttgggctat aataaactct gttaggaaaa
    18421 tttggtataa ttgatagtaa gaatgacaac atgttagaaa ggaagagagc atgcccactt
    18481 tagcagatgt tgcaaaacgt gccaatgttt caaaaatgac ggtctctcga gtcatcaatc
    18541 atccggaaca ggtgactgac gaattgaaag agttagtttt ctcagcaatg gctgaactgg
    18601 attaccgtcc aaatattgct gcaaaagcac ttgtcacgaa tcggtcgcag atcatcaaat
    18661 tatttatact ggaagaaatc gacacaactg agccctatta catgaatttg ctgatgggaa
    18721 tcgctaaacg gatcggtaaa gcgcattatt ctctccaatt agttacaaat gatgccttcg
    18781 acgtcggttc ttgtgatgga tacatcatca caggtgtcag aaaaaaagac ttcgattgga
    18841 tcgaccgttt ggaaaaacca gttgtcttgt ttggggaaaa ttctgaagga tatgattatg
    18901 tagacagcga taatgaatat ggtacagcaa aagcaacgca atatgcctta gaaagagatt
    18961 atcaaacgat tatttatatc ggtatcgaag tggacgagcc ttttgaaatt tcaagggaaa
    19021 ctggttattt atctgtgatg aaggaagttg atcttcctag taaaatcttg cggtttccaa
    19081 atcattcgac agaagtagaa agatatatga cagaaaactg ggcagatttt acagataaaa
    19141 cttgttttgt atgtagttca gatcggctag cactaggagc agtaagaggg attataaatg
    19201 ctggaggaca cttgcccgaa gaatttggtg tgattggatt tgatggggta tttttagacc
    19261 aaatcagttc acctaaattg acaacgatca agcaagatat tatacgtatg ggtgaagtat
    19321 gtggagaaat gttgttaaaa aaaatagaag aaaaaggggc gagtcaaggt tatcgtcgtt
    19381 tttttcctga actagttatc agagagacaa cgaattaagt cttattacta tatatatatt
    19441 tatgaaattc atcgctattt tcttaaaata atcagcgcat tgctactgaa aacaatgaat
    19501 ctgtgctatt attgagaaaa gttccatttt tgagaaggat ggtttgaatg aaagaaatca
    19561 gacgaaaaca tttccgtttt cctgcgtacg atgatgagtt aggcgtaaaa ttaaccaacg
    19621 acaagaagcg agtattattt gacggacaag aagatctttt gactgaacag aatcagtccc
    19681 cgtttgaaca gatgaaagaa tttcatttca cgaacacgaa caccgagaaa atccgtcgca
    19741 agcgtcgtgc aacaaatgga ttgaagtcag ctccgcgtaa ggaaaatttg gaaaagcata
    19801 aaggaaaatt accggattat accggccatt accaatcaga aatcacatcg acaggacgaa
    19861 aaaaactttt tggtgattcc tctcgttctg tcagacaaag tgaaacaagg acagagtcca
    19921 agcatactac acgaaacgca tctgttgaca cgaaggtgcg ggagcgttct tattttgttc
    19981 ctaaatatgt acccgcatcg attatcccag accctagaga aagcaaaatt tctgaggatc
    20041 atttaatcga atcgatgaaa aaaacggatg actcttatct gttgttcgat acagaaccga
    20101 cgccgtatca agaaaaaaga aatggtgagc cgtctgtacg aacattcaat cgaacaaaaa
    20161 cgttagagga aacaaagcag tcagaagaaa aaaaatcgaa aaaaagccat ggtatcttag
    20221 aacgctcatt acaaggattg atcgatgatc aaggaaactc tttgggagaa aatagctatt
    20281 ttaaataagt gatatacaaa tttatattcg gctacagttg gaaaggaaac gctgcggcgt
    20341 ttccttattt tatactctat ccgaaagttt atatcgtatc ctcaaatttt tttgagttta
    20401 agcgaatttt ttctcccaat aagtaagaaa ataatggcaa aataaaaaaa caaactattt
    20461 tcgcagatcg tttgcaaaaa tagtt
//
LOCUS       DARBEC010000052        20108 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.052, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000052 DARBEC010000000
VERSION     DARBEC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.052"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..562
                     /locus_tag="SCL80_001739"
     CDS             <1..562
                     /locus_tag="SCL80_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1107607.1"
                     /translation="LKNEFGIEVKDYFIDLDLLQLINSVKRMYSGDDNGVDLYKFVTK
                     HIFVNLNIDEKNYPSIFKHYLFFINEVEDRIKNNSLSSSVSSFKKMFKSRDGITLTTA
                     HKVKGNEYDTVIAINLLNGKIPHWNEIFNVSDKGISSARKLLYVICSRAKNNLFLFSE
                     VGYFTSKGDLLYPTQLLDKIEFDYNI"
     gene            762..1076
                     /locus_tag="SCL80_001740"
     CDS             762..1076
                     /locus_tag="SCL80_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1107608.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            1097..1474
                     /locus_tag="SCL80_001741"
     CDS             1097..1474
                     /locus_tag="SCL80_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1107609.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            1484..2257
                     /locus_tag="SCL80_001742"
     CDS             1484..2257
                     /locus_tag="SCL80_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107610.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            2279..3682
                     /locus_tag="SCL80_001743"
     CDS             2279..3682
                     /locus_tag="SCL80_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1107611.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3864..5048
                     /locus_tag="SCL80_001744"
     CDS             3864..5048
                     /locus_tag="SCL80_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1107612.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            5045..5335
                     /locus_tag="SCL80_001745"
     CDS             5045..5335
                     /locus_tag="SCL80_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107613.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            5332..5553
                     /locus_tag="SCL80_001746"
     CDS             5332..5553
                     /locus_tag="SCL80_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107614.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5595..6374
                     /locus_tag="SCL80_001747"
     CDS             5595..6374
                     /locus_tag="SCL80_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1107615.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            6441..6935
                     /locus_tag="SCL80_001748"
     CDS             6441..6935
                     /locus_tag="SCL80_001748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1107616.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6991..7629
                     /locus_tag="SCL80_001749"
     CDS             6991..7629
                     /locus_tag="SCL80_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107617.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7701..8096
                     /locus_tag="SCL80_001750"
     CDS             7701..8096
                     /locus_tag="SCL80_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1107618.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            8080..10533
                     /locus_tag="SCL80_001751"
     CDS             8080..10533
                     /locus_tag="SCL80_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1107619.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10530..12557
                     /locus_tag="SCL80_001752"
     CDS             10530..12557
                     /locus_tag="SCL80_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107620.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12554..13576
                     /locus_tag="SCL80_001753"
     CDS             12554..13576
                     /locus_tag="SCL80_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1107621.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13593..14522
                     /locus_tag="SCL80_001754"
     CDS             13593..14522
                     /locus_tag="SCL80_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1107622.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14767..14883
                     /locus_tag="SCL80_001755"
     CDS             14767..14883
                     /locus_tag="SCL80_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1107623.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14899..15702
                     /locus_tag="SCL80_001756"
     CDS             14899..15702
                     /locus_tag="SCL80_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1107624.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15937..16173
                     /locus_tag="SCL80_001757"
     CDS             15937..16173
                     /locus_tag="SCL80_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107625.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            16180..18132
                     /locus_tag="SCL80_001758"
     CDS             16180..18132
                     /locus_tag="SCL80_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1107626.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCL80_001759"
     CDS             complement(18204..18701)
                     /gene="dfrG"
                     /locus_tag="SCL80_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1107627.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            19063..20103
                     /locus_tag="SCL80_001760"
     CDS             19063..20103
                     /locus_tag="SCL80_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1107628.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT         6884 a         3320 c         3901 g         6003 t
ORIGIN      
        1 attaaaaaat gaatttggaa tagaagttaa agattatttt attgacttag atttattaca
       61 gctaataaac agtgtgaaaa gaatgtatag tggagacgac aacggtgttg atttatacaa
      121 atttgttacc aaacatatat ttgttaatct taatattgac gagaaaaatt acccgtctat
      181 ttttaagcac tatctatttt ttattaacga agtagaagat cggataaaaa ataattcatt
      241 atctagtagt gtatctagtt ttaagaagat gtttaaatct cgagatggta tcacacttac
      301 tacagcacat aaagttaaag gaaatgagta tgatactgtt atcgctatta atcttctaaa
      361 cgggaaaatt cctcattgga atgaaatttt taatgtttct gataaaggaa ttagttcagc
      421 gagaaaacta ctatacgtaa tttgttcaag agcaaaaaat aatttatttc ttttctcaga
      481 ggtgggttat tttacgagta aaggagactt gctttatccc actcaattat tagataaaat
      541 agaatttgat tataatattt aaaataccta ggtagtcgtt gttttaaata tagtaggaat
      601 cggacgctca gtacggagcg tctatttttt tacccaattt tgaaaggaag tgaacttatg
      661 aaaataaaaa atcaagaatc aaaaggtcgc tctcccccct ttaaaaccat cacacaaata
      721 agattcattc aaacataaaa acagaaagga taggtaaaaa tatggaactt aaatatgtca
      781 ttcccaatat ggaaaaaaca tttggacaat tagaatatgc tggtgaaggg aacattgaac
      841 aacgtcgaat aaatggcaga ccaaccattc tctctcgtag ttacaattta tactcaacga
      901 ttcaacgagc agatgatatt gttgtcattc ttccctccga agctggcgag aaacattttg
      961 aatccgacga aaaagtcaaa ctcatcaacc cacatattac cgcagaaggt tataaaattg
     1021 gcaatcgagg atttaccaat tacattatgc acgcagacga tatggtgaaa gcataagaaa
     1081 ggaaaggtga aaaaatatga gattagcaga aggtattgtg attgaaaaag accagacatt
     1141 tggcacattg aaattttccg ccttgcgtcg tgaggttcgt atccagaatg aagatggtac
     1201 ggtttccgaa gaaattaaag aacgtaccta tgacttaaaa tcaaaaggac aaggacgcat
     1261 gatacaagtc agtatccccg cttctgttcc tttgaaagaa tttgactaca acgcagaagt
     1321 ggaattaatc aatcctgtgg cagataccgt agcgacggct acttttcgag gggcagacgt
     1381 tgattggtac gtgaaagcag acgatcttgt attaaaagga cagacaaaac ctcatcagaa
     1441 atataaaaaa gaagcgtccg aaagtgaaaa ataggtgttg cttatgacaa acacaccatt
     1501 gattttgaaa gaaattccga aggatgaagc catttctttt attcggcaat accattattc
     1561 aaagatactc cctagattgt gtaaatactt tctagggatt ttttcagaag aaaaattatt
     1621 aggcgttgtg gaattaggct ggggtacgca acccctacaa accatacgca aactctttcc
     1681 cgacagttcc cttcaaacca cagattattt agaaattgga aagatgtgtt ttcttccaga
     1741 aatgaatcaa accaattatt ttggcagtca agctctttca gctctaatta agtggttgaa
     1801 agaacatacg gactgtcatt ttttatatac actggcagat ggaattgagg gaaaatgtgg
     1861 ctatgtctat caagcgtcca acttctttta ctgcgggtat ttcaagacaa gtgtctatcg
     1921 tgataagcag tcatgggaaa agatacaccc tcgaagtgct cgccttttat tagaggaaaa
     1981 tgctcgcttt gaacaggtag agaaaaagca ttggctttcc caggcatttt gcgaatacaa
     2041 agggattgag aaaatcaatg gacgcatgtt tcgctatctc tatccgttga caaaagaagc
     2101 gaaaaaactg ttaggacaca ctctctatcg acgtcattat tatccgaaag aaaaaaattt
     2161 acgctttgaa aagcgaatcg cttatcagaa atatgaagcc atttctcaac caacttttga
     2221 taaacaggct cgtatctaca atacacaatt attttgatag gtggaaagga ggatttttat
     2281 gccattgctt catgacagag gagagcgaat caaagccaac gataagcact tggtctatca
     2341 ttttgtacta atttcattgc tggtagtttt tctagtcgtc ctactccctt ttcattggag
     2401 agcgttacaa tcggtgaact ggcaagcagt tcagttagat acatttttct atcaacttca
     2461 tacgccatac ttttggagca gtattctcac tgctcttttt gtatgcggac ttatcagctg
     2521 gctttattat cactatcgta tagattctat aaaacagtta gagcaccgtc aaaagttagc
     2581 tcgtatgatt ttagaaaatc attggtatga agccgaagaa actcaatcag agggattctt
     2641 taaagattta cccagcaaga aaaccaaaca acgaatccgc cattttccta aaatatatta
     2701 tcgaatgaaa gatggactaa tccatattca tgtagaaatc accttaggga aatatcaaga
     2761 tcagcttttg cacctagaaa acaaactaga aacaggattg tattgtgaat tagtggaaaa
     2821 aacgttgaaa gattcttatg tagagtatgt cctactttat gatactattg gcaatcgaat
     2881 tggcattgaa gacgtggtag ctcaagatgg acgtgtcaaa ttgatggaat cggtctattg
     2941 ggagttcgat tctctccctc acatgttaat tgctgggggc actggtagtg gaaaatccta
     3001 ttttattctc acattgattg aatctctcct gcatacaaat gcaaagcttt atattcttga
     3061 ccctaaaaat gctgatttgg cagacttagg ggcggtcatg aataatgtct attaccgtaa
     3121 agaagatatg ttggcatgta tcaatcaatt ttacgaagat atgattgatc gcagtgaaac
     3181 catgaaacaa caccctaact ataaaacagg agaaaattat gcttatcttg gtttgtctgc
     3241 caattttctt atttttgatg aatatgtggc aatgatggat atgctaggtc gtgaaagtac
     3301 caccgttatt cataaactca aacaaattgt aatgttagga cgacaagcag gcttcttttt
     3361 aattcttgcc tgtcaacgtc cagacgcaaa atattttagt gatgggattc gggacaactt
     3421 taacttccgt gtggctttgg gtcgtatgtc tgaaatgggc tttggtatga tgttcggaag
     3481 cgatacacaa aaacaatttt tcttaaaacc catcaaggga agaggttacg tggatacagg
     3541 aaaaagtgtg attagtgagt tttatacacc ccttgtacca aaaaggtacg atttcttagg
     3601 agaaatagga aaagtcattc aaaaaaagca atctgagcct gtaccgcacg aagtgaaagg
     3661 tacaggttca gacttgcttt gattggtgtg gcgttagcca cgccaatcct ttagccccct
     3721 ctatctaaca ggggggcaca aatacaactg aaaacaggca attctcatca accaatgttg
     3781 atggtacaaa ggattgcgga aaatatgaaa ggtgtaaact caagggtcat agtttacatc
     3841 ttttttagat tggaggtttg cgaatggaaa acgaaaaaat ttggattaag gaattgaaag
     3901 aaaagcgact ggcttatggc gtttctcaaa ataaactggc ggttgcttct catattacca
     3961 gaccttacct atcggatatt gaaacaggca aggcagtccc tactcaacag gtgaaagaag
     4021 atttactgaa cgctttggaa cgcttcaatc ccgataaccc attggagatg ttattcgact
     4081 atgtacggat tcgattccct accaatgatg tggctcaaat tatcggtgag gttttacgtt
     4141 tgaatatgga ttatatgcta cacgaagact ttggctatta ttcttatccc gaacactatc
     4201 gtttcggtga tattgttgtc ctagtctctc atgatgtaag taaaggtgtt ttattagaac
     4261 tgaaaggaaa aggctgtcgg caatttgaaa actttctatt ggctcaacat cggagctggt
     4321 atgatttctt tcaagattgc atggaacata aaggtatttt caaacgcttg gatttagcta
     4381 tcaacgataa aacagggatt ttgaatattc cagatttggc aaagaaatgt aaacaggaag
     4441 aatgcatttc cgtctttcga agctttaaaa attatcgcag tggtgaattg gttcaccgtg
     4501 atgaaaaacc atacatggga aacacacttt atattggctc attaaaaagt gaagtctatt
     4561 tctgtatata tgagaaagat tatgagcaat acgtgaaaaa tgatgttccc ttagaagacg
     4621 cagaagtaaa gaatcggttt gaaattcggt taaaaaatga ccgagccacc caagccatga
     4681 aagatttatt ggcacatcaa caggcggaaa aaacagcctt tgaaattatc aatcgctata
     4741 tccgctttgc agataaagac gacactaaac gtcggagtga ttggaaaact aatgaacgtt
     4801 gggaatggtt tatcggtaaa aatcgtggag cattacgcct aaccacacag ccagagcctt
     4861 attcttttga acgaacattg aactggttac atcatcaagt cgcccctaca ttaaaaatcg
     4921 caagcatttt agatgttctc aacggtacaa ccattatctc caccatgatt caggaagcca
     4981 aactgacaga aaaacatgag aaattaattg aacaacaaca tctagccatg gaggatttga
     5041 ttacatgagc cttttaacaa aagaattaaa gaagttaggg tttcagtgtg gcattgagtt
     5101 tcaagcgtat atacaaaata cagggaaata tacgtctctc attattgagg gaaaacggca
     5161 agctggtgat accatctata cctatgattt ttacaaggtg agattttata ataattatac
     5221 caatcgagta accgtctatg gagagcattt aacccctttt caattattaa ggcgtgtaaa
     5281 aagctatatc tattatcgag aaaaatatct taaggaaagg aggacaatca catgaatttt
     5341 gggcagaatt tgtacaactg gttcttggat aatgcacagt cattggtatt gttagcgatt
     5401 gttgtcattg gactgttttt aggttttaaa cgagaatttt caaaactcat tggcttcttg
     5461 attattgctc taatcgccgt cggacttgtg ttcaatgcag ctggcgtgaa agatgtgtta
     5521 ttaaacttat ttaaccgaat cattggtgct taacagatat ttttcatgat aaaataggaa
     5581 gtatatattt tgttatgaaa ggatttatga atatgtttac aaatgatcaa tttaaatctc
     5641 ttttaattaa aacattagaa agaaagagct cttcaaatta ttatgaagga ggtaacgaga
     5701 ttgtttctaa aatgaaaata tcagaggtaa ctttagatga gactgacgat tttacttatt
     5761 ataaaggata taaaaagggc tggaatacgc tttgcacata tcttaaaata agagttcctt
     5821 ttgatgattt agactttttt gaaagtcata aagatttgat tacccagaca gcaagctcca
     5881 tatatgataa acaaggtgat aatgtattag tggatactat tttagtacca cttccagaga
     5941 attacgaagt aattaacttt agtcagttga aaattagtga tgtggtttca caagctattg
     6001 aagacgctga gtcttttatg agtaatggtg aatatcaacg tgcctttgac agagttcaca
     6061 ctgcttttca tggatattta attgaaatac taaaaaaata tgaaattaca gttcctagag
     6121 acgaaaatct atctaaatta tatagtagga tacaacaatt aattgaaaaa gaaattcaac
     6181 ctactgaact tgctgatata gtgaagacaa cgataaggtc atcaaatgga atgattagct
     6241 ctcttaatga agcaagaaat aggcattcac ttgcacaccc taatacaaat attattggaa
     6301 agagagaagc aaaactaata ataggtattt caagtacagt tacagattat attagcggat
     6361 accttgataa gtagcgtcat taactgattg ctactttttt tatgactatt tttataaaaa
     6421 ggatgtgata agaattggaa atgaaagtct atgtggcaaa tttaggacgg tataatgaag
     6481 gtgaattagt cggagcatgg tttactccac caattgatga ggaagaaatg gcagaaagaa
     6541 tcggtttaaa tgaggactac gaagaatatg caatacatga ttttgagctt ccttttgatg
     6601 tggacgaata cacgccgatt agtgaaatca accgtctatg tgaagccatt caagaaatcg
     6661 aaggtacacc tatttataat gaactcaaag aaattcaagg catgtggttt agtagcttgg
     6721 aagaattact ggaaaacaaa gaggatattc attgttatag tgattgtgat tcgatggaag
     6781 atgtcgcccg ttattacgtg gaagaaacag gacagcttgg tgaagtccct tccaatttac
     6841 aaaattatat tgactaccaa tctttgggac gtgatatgga aatagagggt aattacctag
     6901 ttacctctca tggggtattt gaatattgtc agtaagttta acaatccgtg ttataattaa
     6961 attaataaat agcacggaga ggtataaatt atggtaaatg tatttgaaaa tcttaaacta
     7021 ttaaaactag acatgaaaga taaaggctgg gtgatagatt ccttttactt tcgttacaaa
     7081 cagcaaaatt atattgtact tgttaagcta tttgaaaaag aggaaaaagt tccagaatat
     7141 gctctgttaa aactagaatt tttaaaggaa aacgattttt cagatatgct agctgtctat
     7201 gctaattcgg taaaattata tacagacacg aaaacaataa gagaatattt tggtatcgaa
     7261 tacagtagta acttgggaga tgttttattt caatttagcc aaactcttgc tcgatttatt
     7321 ccgacagaag ttagtgagaa gaaaaatgaa gaccaaaaag aagccatgtg tttctcatta
     7381 agtcaaagtg attcagaaga tcccagaaaa aaatattgtt tttcagttag aagaaatcct
     7441 ttaaagggaa atggtgagct gggaaaacga agtcctttta atgacaataa aacgagattg
     7501 tatagaccca gcttatatga acgattaggg tcagatacca atttaagttt cagatattca
     7561 atgaatccag atgatgaaga aactgatgaa ggtattattg caaaatggac aaaaaataaa
     7621 attgaatagt taaagcattg aaagagcaat cacctatttt gattgttctt tttctatttt
     7681 tacggaaagg acgtgaagac atgaaaaaaa taagaagtta caccagtatt tggtcagtag
     7741 aaaaagtgat ttatgcgatt aatgatttac aactcccttt cccaattacc tttacgcaaa
     7801 tgacatggtt tatcgtttct ttgtttgtcg tcatactctt tggcagttta ccaccacttt
     7861 ctctgattga cggagcacta ctcaaatact tagggattcc cattgttctc actgggttca
     7921 tgtgccaaaa gacctttgac ggaaagaaac cctatggatt tctaaagtct atcattacct
     7981 atgctctacg ccctaaagta acctacgctg gcaaacaaat acgattaaaa aaagaaacaa
     8041 tgaatgaatc gattacgatt gtaaggagtg aacaactaga tggcatatcc gattaaatat
     8101 atggaagata atctcgtatt taatcatgat ggcgaagtat tcgcttatta tgaattaatt
     8161 ccctataatt acagtttttt aagttccgat gaaaaaattc aagttcatga taatttctgt
     8221 caattgattg cccaaaatcg tgatggcaag attcatgctc tgcaactagc aaccgaatca
     8281 agtattcgag ccacacagga acaatcgaaa aaagaaatca ctggacgatt aaaagagata
     8341 gcttacgaaa aaatagatga acagaccgaa gcgttaattt ctatgattgg tgacaatcaa
     8401 gtagactatc gcttttttat tggttttaaa ctctacctca atgaacagga agtatccgta
     8461 aaaaatgttg ggaaagaaat tcaaaaagca tttgcggaat tctttcatgg tgtgaatcac
     8521 aaattaatgg gcgattttgt ttctatgcct atggcggaaa tcgaacgctt ttctaagatg
     8581 gaacagttgt tagaaagtaa attatccaga cgctttcaga ttcgtaaatt ggagaaagat
     8641 gattttggtt atattattga acatctttac gggcaaacag gtgtcccttt tgatgactac
     8701 gactaccatt taccgaaacg atacgaagat aaaaaagtgt tggtaaagaa atacgatatt
     8761 ctcaaaccca ctcgctgttt aatcgaagaa aatcaacgct atttacggat tgaacaggaa
     8821 gataccacta cttatgtggc gtattttaca attaatagta ttgttggtga attggatttc
     8881 cctaatagtg aaatttttta ttaccagcaa cagcagttta ctttccccgt tgataccagt
     8941 atgaatgttg aaattgtcgc caataaaaaa gccttatcta ccgttcgcaa taaaaagaaa
     9001 gaactgaaag atttagatag ccacgcatgg gaaagcgaca acgaaaccag tactaatgtg
     9061 gtggacgctt tagaatctgt cgatgaatta gaaagtaatt tagaccaaac caaagaatct
     9121 atgtacaaac taagctatgt ggtacgggta tcagcaaaga atttggacga gctgaaacaa
     9181 cgttgtgatg aagtaaaaga tttttatgat gatctgtcta tcaaattagt acgacctttt
     9241 ggcgatatgc taggcttgca taatgaattt attccctcaa gcaagcgata tatcaatgac
     9301 tatattcaat atgtcacgtc ggattttttg gctggtcttg gtttcggtgc tacacaaatg
     9361 attggtgaaa attctggtat ttacgtcggt tacaatctcg atacaggcag aaatatttat
     9421 ctccaaccta gccttgcaag tcaaggaatt aaaggctctg taacaaatgc attagcgtcc
     9481 gcttttattg gttcattagg tggcggaaaa tcattctcta acaatatgct ggtctattat
     9541 tcagtactct ttggcggaca ggcaaccatt gttgacccaa aagctgaacg tggtgaatgg
     9601 aaagaaacct tacctgatat tgcccatgaa atcaatattg ttaatctcac gagtgagaac
     9661 gacaataaag ggttacttga cccttatgtg attttaaaac tcccgaaaga ttcagaaagc
     9721 cttgccattg atattctcac gttcttaaca ggtatttcca gccgagatag cgagaagttt
     9781 cctgtcctgc gtaaagctat tcggagtgta actcaaagca aacaacgagg gttgttattg
     9841 gtgattgatg aattgcgaaa agaagatacg cccatcagca atagtattgc tgaccatatc
     9901 gaaagctttg tcgattatga ttttgcccat ctgttattca gtgatggcag tgtcacccaa
     9961 tccattagct tggaaaaaca actcaatatt attcaagtgg cagatttggt cttacccgat
    10021 tccaatagct cttttgaaga atacactacc atggaattat taagtgtggc aatgctgatt
    10081 gtcattagta cctttgcttt agactttatc cactctgacc gttccatttt taaaattgtt
    10141 gaccttgatg aggcttggag ttttttacaa gtggcacaag gaaaagctct atctatgcga
    10201 ttggttcgag caggacgtgc catgaatgca ggggtctatt tcgttactca aaacgcggac
    10261 gatttactag atgaaaagat gaagaataat attggcttga aatttgcctt tcgttccact
    10321 gacctagtgg aaatcaaaaa gaccttagaa ttttttggtg tggacaagga agatgaaaac
    10381 aatcagaaac gcttacgtga attagaaaat ggacaatgtt tagtttcaga catctatggt
    10441 cgtgtagggg ttatgcagtt tcacccgatt tttgaagagt tgcttcatgc ctttgatacc
    10501 agaccgcctg tgcgaaatga ggtggaagaa tgacacaaaa acaaaagaaa tttcttcact
    10561 atagttggat aacgctcctt tgtgtgggcg ttattttact tttattaggc acgctgggca
    10621 atgcagtaca agcgacaggc ttagttgatg aaaccattga tacaagcaat gaatactcca
    10681 agtatggttt gaatcaatat cagcttgatt attatgtgga caatagctgg ggctggcttc
    10741 catggaattg gagtgatggc ataggacaat cggtgatgta tggattgtat gccattacta
    10801 attttatttg gacaatttct ctttatctat cgaacgccac aggttatttg gtgaaagaag
    10861 cctatgcctt agatttcatt agtcaaacat cccaagcaat tggcgaaaac atgcagacga
    10921 ttgcaggcat tacaccgaat ggcttttcca gttcagggtt ttatgtcggt ttcctccttc
    10981 tttttattct cattgttgga atctatgttg cctatacagg attggttaag cgagaaacga
    11041 cgaaagtgat acgagctgtt ttaaactttg tcgtggtgtt tattctatcg gcttccttta
    11101 ttgcgtatgc acccgattat attgcgaaaa tcaatgattt ttccaaagat gtcagtaccg
    11161 ccagtctgaa tattgggaca aaaattgttc ttcctaacac ggatagtcaa gggaaagaca
    11221 gtgtggacat gatacgtgac agcttattct ctgttcaagt aaagcaacca tggctattgc
    11281 ttcaatatgg ctccactgat gtagaagcgt taggggaaga acgtgtggaa agtttattgt
    11341 ctaccagtcc agatacaaac aatggtgaag atagagaaaa tgtagtgatt gaagaaattg
    11401 aagatcatga caatacctat ttaaccctac cgaagacgat ttccagatta ggaacagtct
    11461 tctttttgtt tgtattcaat attggtatct cgatttttgt tttccttcta acaggaatca
    11521 tgattttttc gcaagtgcta tttattattt tcgctatgtt tttgccaatc agtttcctat
    11581 taagtatgtt gccgagtttt gatggcatgg gcaggcatgc cattaccaag ttatttaatg
    11641 tgattatgac aagggctggt attaccttga ttattaccgt tgcctttagt atttccacta
    11701 tgctttattc gctgtcagca agttctccct ttttcatgat tatgttttta caaattgtga
    11761 cctttgcagg gatttatttt aaactgggcg atttaatgag cctattcact ttacaaagca
    11821 acgactctca aaacatgggc agacaagtgt ttcgcagacc tcgtcaaatg atgaatcgac
    11881 aaaccagaag atttcaacgc agtatgagta agatttttcg tggaaaagca gggtcaacga
    11941 agcaaaagac aatgacaaca aagcagaatc aaaccaataa agctaaccat actcgtcaaa
    12001 atgaacggac tacaccaaag aaagaaacag cactttccaa tgtgaaaaag caacaaggcg
    12061 gtatcgggca acgattaggt gaaaagacag cccatgtatt ggacacgaaa aatcgtctag
    12121 tcaataaaag aaagcaggca aaacaacaga tacaggcgac accaacccat gtcaaatatg
    12181 cccttcatca aggaaaagag aatgtcactc ataatgtaac agactttaaa caatctatgg
    12241 cagataccaa agctcaacgc aaacaacagc gtatgaacca gcaggagcaa aaacgaaaga
    12301 acattgctca aaagcgtttg gagctggata aagcaaaaga aaaaagacga aaagaaaacc
    12361 catcatctcc tgttcagaat aaccagcagc atgttcgcaa gcgtcagcaa accgtcacgc
    12421 ctgttttgtc acaaaacact aaacgtgaaa atcatcagcg accattgaaa aaagagcaaa
    12481 aacaaaagac accaccgaag caattcaatc agaggaagct aaataaaaga acggataagc
    12541 gaggacaaca aacatgaagc taaaaataac agtgatttgt agtgtggtct tctctctctt
    12601 cttttttctc ttattattcc tagtcatttt ctttgcagat gatacggaca gtggcgaaaa
    12661 caacaaggat tcttctattc cacaaggcgg tgtgaccgtt tcaccagaag tgttggcaca
    12721 ccgtccgttg attgaaaaat atggtaagga atatgggatt gaagactatg tttcttatat
    12781 cttagccatt atgcaagtgg aatcgggcgg tacagcagaa gatgttatgc aaagttccga
    12841 atctttgggt ttaccgccca acagtttgag taccgaagag tccatcaaac aaggggttaa
    12901 atatttcagt gaattattaa ccagtgcaga acaacaagga gtggatatag attctgttat
    12961 ccaatcttat aattatggcg gtggcttttt aaattatgtg aggagtcatg ggaaaaaata
    13021 tacctatgaa ttagccgaac agttttctaa agagaagtcg ggtggtcaaa aagcagacta
    13081 tcccaatcct atagccatac ctgtgaatgg tggttggcga tataactatg gaaatcagtt
    13141 ttatgttcag ttggtttcac aatacctaac cgacacttcc ccaacagaat ttgatgatga
    13201 aaccgtgcaa gtcattatgg acgaagcctt gaaatacgaa gggtttccct atgtctttgg
    13261 aggggcgtcg cctaccacct cttttgattg tagtggctta atacaatggg tgtatgacaa
    13321 agctggcatt tctctccctc gtgtcgccca agatcagtat gacgctacgc aagaaatctc
    13381 tatggaagaa gcccaagcag gcgaccttat cttctttcat tcaacctata atgcaggcac
    13441 atatgtgact catgtggcga tatatttaga gggaaatcgc ttttatcatg caggcgaccc
    13501 aatcggttat ggcgacttaa gcagtcgtta ttggcaagac catctgattg gggctagacg
    13561 tgttattcat aactaagaaa ggaatgaatc aaatgattat aaaaatcgag cgaaaagaaa
    13621 agaaggagaa accaaagaaa tctaaaaaac cgagcaagca aagaaaagtt ccgatgatta
    13681 aagttggcac gcataagaaa ttaacctttg ttttatgggt tttattgatt ggtagtgtcg
    13741 gatttggaat ctataaaaat tttactgcca ttgacactca tactgtccgt gaaacagaaa
    13801 tcattaaaca gcagattgtc gataccaacc aagtagaaag ttttgtgaaa tcctttgcga
    13861 aagactactt ttcgtggcaa caatcccaag aagccattga taaacgaaat gaaaaattga
    13921 cccattattt gacggaagaa ttgcaggtat taaatgaaga aatgattcgt aaggatattc
    13981 ctactagttc tagtgtgaac gatatacagg tgtggcaggt ttctcaagtg aatgagaaca
    14041 cctttgaggt actgttttct gttgaacaag ttattaccga agacaaagac aaggaaacta
    14101 tatcgtctag ctttcatgtg gttgtccata tagatgaatc agataatatg gttattatca
    14161 aaaatccgac aatgagtaag aaacctcaaa aatcggacta ccaacccaaa cagctagaaa
    14221 gtgaccatac agtagataca gaaacgatgg acgaaatcac tagcttttta gaaacgttct
    14281 tccagctcta tcctaccgcc acagaaaaag aattgactta ctatgtaagt aatcatgtgt
    14341 tacctatgat taacaaggaa tatgttttcg aggaattggt gaatcctatc tttaccagaa
    14401 aagataacca agtaatcgtg aatgtcgctg tgaaatattt agatcaagaa acaaaggcaa
    14461 cccaaatctc gcaatttgag ttgatattag aaaaacaaga taattggaag attgtaaaat
    14521 aacaaatatt ggtacatgat tacagatact ttgtaatcat gtactctttt tgataaaaaa
    14581 ttggagattc ctttacaaat atgctcttac gtgctattat ttaagtgact atttaaaagg
    14641 agttaataaa tatgcggcaa ggtattctta aataaactgt caatttgata gcgggaacaa
    14701 ataattagat gtcctttttt aggagggctt agttttttgt acccagttta agaatacctt
    14761 tatcatgtga ttctaaagta tccagagaat atctgtatgc tttgtatacc tatggttatg
    14821 cataaaaatc ccagtgataa aagtatttat cactgggatt tttatgccct tttgggtttt
    14881 tgaatggagg aaaatcacat gaaaattatt aatattggag ttttagctca tgttgatgca
    14941 ggaaaaacta ccttaacaga aagcttatta tataacagtg gagcgattac agaattagga
    15001 agcgtggaca aaggtacaac gaggacggat aatacgcttt tagaacgtca gagaggaatt
    15061 acaattcaga caggaataac ctcttttcag tgggaaaata cgaaggtgaa catcatagac
    15121 acgccaggac atatggattt cttagcagaa gtatatcgtt cattatcagt tttagatggg
    15181 gcaattctac tgatttctgc aaaagatggc gtacaagcac aaactcgtat attatttcat
    15241 gcacttagga aaatggggat tcccacaatc ttttttatca ataagattga ccaaaatgga
    15301 attgatttat caacggttta tcaggatatt aaagagaaac tttctgccga aattgtaatc
    15361 aaacagaagg tagaactgta tcctaatatg tgtgtgacga actttaccga atctgaacaa
    15421 tgggatacgg taatagaggg aaacgatgac cttttagaga aatatatgtc cggtaaatca
    15481 ttagaagcat tggaactcga acaagaggaa agcataagat ttcagaattg ttctctgttc
    15541 cctctttatc atggaagtgc aaaaagtaat atagggattg ataaccttat agaagttatt
    15601 actaataaat tttattcatc aacacatcga ggtccgtctg aactttgcgg aaatgttttc
    15661 aaaattgaat atacaaaaaa aagacaacgt cttgcatatt aagtagttca gttttggagt
    15721 acaaaaagtg aaaatgtaaa cctaaatgag tatcaaattg caactgaatt tgatactcgt
    15781 tttttattgt taaatcaacg tttgttgtta atttacacca caaaatgtaa gttaaaatgt
    15841 atataaaaat gcgaacgtga aataattatt gtaggaatgc aaattggtgt gttaaaatgg
    15901 ttacaattta ctaagcaaaa aggggtgcat tttcctatgc aatttcttga gccaaaaaat
    15961 aagaacgcta agtctgtgga ttgggaaata tcagagcaag taagggtgat agttaagcaa
    16021 tacgctgaat atgcagagcg tactgaaagc gaagcagtgg atgaattctt attgaacatt
    16081 ttagacgata agaagtttat tgaatggatt gccaacaagc gaagtaataa gcggattgtt
    16141 gaaaaaatgg gtatcaaaga tagagtgggt tgatagacaa tgaccaagct taaaagatta
    16201 gccacgaagg aagatgaaat tgtagatgtt aaaattccga tttcaaacga agaattaaaa
    16261 gatagggcta aacaatatga tttattaact ccaaaacgat ttgcaacaag gtataacaaa
    16321 atgttgttct tgccaacgtc ttttaaatgg aacggttctg aatatcccat tcaatataat
    16381 tactgcatca atccgttttg ttgtaatttt gggaaagaac agcacaaatt caaagatgta
    16441 aaagggaaac caagtcgata taaaatgact ggtagttcca aagataaagg tcataaaggt
    16501 atgtactgca atgacaatcc tattggcaga ggtgtatcgc aaaactgtac agtaacccct
    16561 ttatctaatt ggtctgttgt tgaagaaatt aaacgcctta tagaaatcaa tagcatccaa
    16621 gatgttgagc cagattatca atttcataaa gaaggctgct cggaagaaga atcaacccca
    16681 ttcaatgagc cgaagcaatt ttacaaaagg gggaaaagta ggggcaaatc tcaacgctat
    16741 caatgtaagg cgtgtaagaa gttcactaat gttctaccaa aaagagaaga aaccactact
    16801 tatcaccaac aaaagaacac cattcttcca atgttcgcta agatggttgt tggaagggtt
    16861 tcagtcagtc gtacttgtga tattttagga attggagttg gaacctacta tcataagctg
    16921 gagtggctgt ataggcgttg tttggagttt ttagaacgat atgagacaca accccttcaa
    16981 acaaagaaat ttaacgagat gtggcttaat acggacaaga tgcactacta cctaaataac
    17041 gtccgtaaga aaggacaagg ttcaaagaaa tatacggggt ttgaagacct taatatgcaa
    17101 acgtatatag ttgtatctgc tgaggtctta tcaagatatg tgttccgttc tgatgttgcc
    17161 tatgattgga atatttcaat ggatgaatta aatgaagata caagaaaatt caaagaagac
    17221 catctcaata cttttagtcg taaaaatgat aggttagatt ggtcttatta tccacaagaa
    17281 ccatccgcta acgatagcga aaataggaat gcttatcttc acgaattagg gaaaattact
    17341 aataggagta gatttgtcga tggtttaaat gttgatgctc cttatactac aactgctcac
    17401 tattggttaa ttaaacaaat ggttaatgcc gacgaatggc gaatgattag tgatgatgac
    17461 ttctctatta gaaacgcctt ttatagagta ttcacgaaag aattaaggtt gagcgatgca
    17521 catcatttta tttgccaagt taacaagacg aaaagcagaa aacaatgctt aaaagaattc
    17581 ggacaagcaa aagcagaact gctcgattgg ggagatatca gaggattcaa aacaaagttt
    17641 ctacggactt tggcatctca ttatctaact gaattactaa caagtcatca attccacgaa
    17701 gaggctatta gtaaagatgg agaaagatat cgcaaatatg ccgataaccc tatcaaacat
    17761 ccattagcca ctaaggataa aggattttat tcagttgatt gtagaacgga tttatctgct
    17821 ctggaaccaa acgaaatcgc aaagatgcta ttaaatgtta atgaccattc gaccaatagc
    17881 tttattcaac agataagacg ttatatatca tctcttgaaa gaccattaac aacagctcgt
    17941 ggtgataaga agagctatat ttacgctaac ttcaatccga agtatgctca atttgcgatt
    18001 acaattctaa gaacttacta caacttttgt cgccctttta aatcagcgga taaaaaggtt
    18061 ttgacacctg ctcaaagatt agggattaca gataaacaat ttgattggaa agatataatt
    18121 tatttcaaat gacaaaaaga gcttggagta atcccaagct cttgtgcttt atctttactg
    18181 ttcttcttca attaacgcac ccgttaactc aataagtttt ttctttcata tacatgaaaa
    18241 tagtagttat acggattttt atcattcttt atcccttttt gggcaaatac ctcattccat
    18301 tcctcataat tcacttctgg aaaaaaagta tctccttcga attcatgatg tatttttgtg
    18361 atgtacattt tctcaacata agggaaaaac aaattataaa tctgttctcc tccgaaaata
    18421 aaaatttctt cttcgttttt acataactca aaaacatctt ctattgaatg aacaatttca
    18481 caaccattaa aggtaaaccc cttatctctc gtcagaataa tatttcttct gtcaggtaag
    18541 gctcttccga ttgattcaag gttcttccta cctaatatta tcggatgtcc ctttgtagta
    18601 tttttaacat attcccagtc cttgggaatc ctccaaggaa tgtcattctc tttgccaatc
    18661 actctattct tatccatcgc agcaatcaaa gaaactttca tttataacac agccttttta
    18721 actaatttac catctaattt cttatttcta tcaaaaaacc ttttctcatt atcctgcccg
    18781 ttagttgaat aagcaatttt ttctataagt tatattatca taataccaca aaatcacaaa
    18841 ttttcattca attatcattt tcaattgcat tttataaatc attttgtatg taatttggta
    18901 tgtaagttaa aatgaaaaaa gtagtccatt ttgaagtaca ttttgtcatt ttgtactcca
    18961 aaactgaact acttacttgc atatatacgc ctttatagtg gagtactaca tttacgagat
    19021 tcggttagag tatcagaaaa agaaaaaata aaagttacag aaatgtatac ttcaataaat
    19081 ggtgaattat gtaagattga tagagcttat tctggagaaa ttgttatttt gcaaaatgag
    19141 tttttgaagt taaatagtgt tcttggagat acaaaactat tgccacagag aaaaaagatt
    19201 gaaaatccgc accctctact acaaacaact gttgaaccga gtaaacctga acagagagaa
    19261 atgttgcttg atgccctttt ggaaatctca gatagtgatc cgcttctacg atattacgtg
    19321 gattctacga cacatgaaat tatactttct ttcttaggga aagtacaaat ggaagtgatt
    19381 agtgcactgt tgcaagaaaa gtatcatgtg gagatagaac taaaagagcc tacagtcatt
    19441 tatatggaga gaccgttaaa aaatgcagaa tataccattc acatcgaagt gccgccaaat
    19501 cctttctggg cttccattgg tttatctgta tcaccgcttc cgttgggaag tggaatgcag
    19561 tatgagagct cggtttctct tggatactta aatcaatcat ttcaaaatgc agttatggaa
    19621 gggatacgct atggttgcga acaaggatta tatggttgga atgtgacgga ttgtaaaatc
    19681 tgttttaagt acggtttata ctatagccct gttagtactc cagcagattt tcggatgctt
    19741 actcctattg tactggagca agcctttaga aaagctggaa cagaattgtt agagccatat
    19801 cttagtttta aagtttatgc accacaggaa tatctttcac gggcatataa cgatgctccc
    19861 aaatattgtg caaatatcgt aaatactcaa ctgaaaaata atgaggtcat tattattgga
    19921 gaaattcctg ctcgatgtat tcaagattat cgcaatgatt taactttttt tacaaatggg
    19981 cttagtgttt gtttagcaga gctaaaagga tatcaggtta ccactggcga acctgtttgc
    20041 cagacccgtc gtctaaatag tcggatagat aaagtaagat atatgttcaa taaaataact
    20101 tagtgcgt
//
LOCUS       DARBEC010000053        19837 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.053, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000053 DARBEC010000000
VERSION     DARBEC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19837)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19837)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19837
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.053"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            202..1077
                     /locus_tag="SCL80_001761"
     CDS             202..1077
                     /locus_tag="SCL80_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107629.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(1222..1374)
                     /locus_tag="SCL80_001762"
                     /pseudo
     CDS             complement(1222..1374)
                     /locus_tag="SCL80_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(1642..2973)
                     /locus_tag="SCL80_001763"
     CDS             complement(1642..2973)
                     /locus_tag="SCL80_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1107630.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(3122..3835)
                     /locus_tag="SCL80_001764"
     CDS             complement(3122..3835)
                     /locus_tag="SCL80_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1107631.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            4010..5434
                     /locus_tag="SCL80_001765"
     CDS             4010..5434
                     /locus_tag="SCL80_001765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1107632.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            5733..6440
                     /locus_tag="SCL80_001766"
     CDS             5733..6440
                     /locus_tag="SCL80_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEG1107633.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            6427..6750
                     /locus_tag="SCL80_001767"
     CDS             6427..6750
                     /locus_tag="SCL80_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEG1107634.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(6839..7876)
                     /locus_tag="SCL80_001768"
     CDS             complement(6839..7876)
                     /locus_tag="SCL80_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1107635.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(8197..9180)
                     /locus_tag="SCL80_001769"
     CDS             complement(8197..9180)
                     /locus_tag="SCL80_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEG1107636.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(9595..10365)
                     /locus_tag="SCL80_001770"
     CDS             complement(9595..10365)
                     /locus_tag="SCL80_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107637.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMISGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(10358..11293)
                     /locus_tag="SCL80_001771"
     CDS             complement(10358..11293)
                     /locus_tag="SCL80_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107638.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(11609..13258)
                     /locus_tag="SCL80_001772"
     CDS             complement(11609..13258)
                     /locus_tag="SCL80_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEG1107639.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(13375..15978)
                     /locus_tag="SCL80_001773"
     CDS             complement(13375..15978)
                     /locus_tag="SCL80_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEG1107640.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(15990..17039)
                     /locus_tag="SCL80_001774"
     CDS             complement(15990..17039)
                     /locus_tag="SCL80_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEG1107641.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(17065..17379)
                     /locus_tag="SCL80_001775"
     CDS             complement(17065..17379)
                     /locus_tag="SCL80_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEG1107642.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(17395..17841)
                     /locus_tag="SCL80_001776"
     CDS             complement(17395..17841)
                     /locus_tag="SCL80_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1107643.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(17842..19764)
                     /locus_tag="SCL80_001777"
     CDS             complement(17842..19764)
                     /locus_tag="SCL80_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEG1107644.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
BASE COUNT         6063 a         3963 c         3226 g         6585 t
ORIGIN      
        1 ataaataaaa aacaaaaaaa gttccatctt taaaacacgc tattctttta ctttttctca
       61 cattctctaa gaaaagaaaa ggagaatgaa tttttgacaa ttcgagcaaa agtgattatc
      121 aaatatgtaa aatcagctac ttttttctta aacgtttctt tatactaaat ataaataagt
      181 gtttcatgaa aggagaacta gatgaataat tggcttgagt attttcctga aaacgttttg
      241 gaacgcggtt acagctatca tctgcacggt tttgtccgtc atttgaacta tacaagcaag
      301 tacttgtccg caacagtttc tggcacggaa gattataagg tagtgatcac ttgggatgaa
      361 aagaccaaca tgacttgtga ttgtctatat gccatagaag gaaaaaaatg caaacacatg
      421 gctgctgtct tattcgctta cgaagaacgt ccaataaaaa aatccaacta ctcactttcc
      481 gaactctcgt cccttgtttc ctccgctagt tcctctcttg tacgggagct tttaacagag
      541 attcttatag aacatccgca atttatcgaa cgcttcaaag ttaagatgcc atttcatgca
      601 ataaattatt cagataaatt aacaactatc atccataaat acgatcatat tatcaagaaa
      661 aacaaaaaca ggaaaacagc gaagttcatc atggagatgc gaaagttcat ccaagaagca
      721 gttgaatcgc tcattcagca aaatgcctat cttcccgcat tcgaattgat caatgaagtg
      781 attgctactc tcgaaacatt ctattgggag cctgaggatg aacgaaccct tcttctgata
      841 gaggactgtt attatttatg gaaagaatta ttagcagaag cacctcatgc agaaaaaagg
      901 caaatgttct cttggttcgt ctgccaagtc gatcatactg acgcctctta ttcaaaacgc
      961 tactccataa aaatcctgaa agaagacttt cgggaaaaag aattttctaa ccaaaagaaa
     1021 aaaatagata aaaaaactaa agaaacggta aagaaagacg atttcaatga aaaatagcaa
     1081 gtaaacgata acaaccatcc taaatccaca agaaatacag cagaccttgc tttaaaaaca
     1141 cgaatcatgc aaaggatgaa gtaaagaaga cttaacgcgt acccaatatt agtcatttga
     1201 ctattaggta cgcgttttgt tttataagtc atgacaaatt tccttcgttg ctgggtagat
     1261 tttccggcat tgttgataga ttttatgata cttttctaca ttttgtaaga gaggttccac
     1321 tgcatctgta tattggataa aattctgaca acaactttca ggtgtgtcga accactctaa
     1381 tctttaaaga taaatattaa aataacgatt aataatatta acagtcttta tttttcacca
     1441 ccaaaaacct atctttgcat atactaaata gacgtatata cacagatagg tttgtcttgt
     1501 gaaaaataat gattatcctt accaaaaaat gtttgttttt ctacatgttc gtacttatca
     1561 aaaaatatga tccttattga aattactggc tatttatatt ccaacgactt cttgtgttaa
     1621 tttttgttat ctcatagtaa attatgtttc attttcactt aatttagttg tcacgtgttc
     1681 gtcaattcgt aaaaacggta aatatataaa cacacttgcg gctataatta atatttgtaa
     1741 aacagctgct cgccagtctc cggctgttgc taaaaatccc gatataactg gtggcgtagt
     1801 ccaaggaatt tgcactacta catgattaat taatcccaca gcagttgcaa aatatgctac
     1861 tgtcaaagaa aaaatcggtg caagcacaaa gggaataatc agactgatat taaatacaat
     1921 aggcaatcca aaaataatag gttcgcttat attgaatata gcgggagtta ccgataattt
     1981 agcaaagtcc ttataatctt ttctacgact gaagataaat attgcaatca ataaagcaat
     2041 cgtatttccc gatccaccca ttaccgcaaa ggtatcttta aaggccatat tgataatatg
     2101 tggaatttgc tctttgtttg cataagctgc catattttct tgcatattct gaatcaaaaa
     2161 cggatctaac aatgaacctg atattactgc ttgatggata ccaaatccaa aaaatagatt
     2221 agcaatagat gtaattaaca aaaatcctgg taaaccagtt gtaattttac ttaatggctc
     2281 tgtaactatc gtggtaatta gcttatttat atccatatta aacaattgaa tgattataaa
     2341 tgacaataaa gcaaacgagg taatcgtaat aatgattggg attaatacat taaaagactt
     2401 taaaacagcg ggaggaatat ttccggatag ctgaatctgt agtttcttat tattagaaag
     2461 tttaataaat aattctgtag ccgctaatcc aacaaatata cctacaaaca ttcctgaagc
     2521 tcctgtatag gatataggta tcacacccgt cacttcaaca gctttatccg cattttcagg
     2581 agtaaacatc atctgcatag gtgttaatac aacaaatgtc gaaatagcaa ctaataccgc
     2641 tgcaatatga tttgtataat ttctgcttgc acacatatga taagatactg ctgctacaac
     2701 taataatgta gttatgctta atgtaccatt ggcaattgaa gtaccaattt gttgtaacgt
     2761 ggtcaatgta ctagtatcga caatttcccc catgaatcct gttggttcta agatcactga
     2821 attaataagt gtcacaaatc ctgctaacat cataaatggc ataaatgtag caaacgcatc
     2881 tcgcaaagtt cttaaatgga tctgattccc caacttagtc gcaactatac caaatttttc
     2941 aaatacaatg tccttcctgc tacttttgcc catatttttt ctcctttatg cttattgtaa
     3001 tcgattactt tttccaccca aataatacat cacttatcaa aaaaagtaaa tacttttaat
     3061 taaatatata tatctttttt agtataaaaa aataagctct gaaaaaataa ttttcccgag
     3121 cttatttttc aacaaaaaca gcttcaaaaa caaagtcttg atataaatgt ctcgataccg
     3181 aatactcgaa aggtttacca gacgatagag caacaacttt gtctatctca atcataaatt
     3241 cacctggatc tatttttaat atttttgcgt caaattcatt cgcagtatct cctttgatac
     3301 gtatgtgcga agtctttatt tccagaccca gttcatttgt gatataagca tatacagatt
     3361 tctttaaatg ttttggttcc aagcctggta tgactgctaa tggcatgaat gtttgctcaa
     3421 tcgagtatgg ttgacaatca ataaaacggc ttcgtacaat attatataca tagtctttta
     3481 cactgatact taatttatca gctacttttt tcgatggaag ttcaattgag aaagtatgga
     3541 tttctgaatc tacgtgttct tcaccaacca cgctaaacaa accttcgttt ggaccaagaa
     3601 ctcgtgagtt atcaattggt gttctgacga cttcagtccc atacccactt ctacttcgca
     3661 aaactccttc ggaaacaagt aagtctacgc ctttctttac tgttaacttg cttgtatgat
     3721 aaataactgc caattcatct cctgtaggaa gtttctcacc aattttatac tctccattta
     3781 caattttttt ctttatatct aagtaaattt ccatatactt cgcaattttt ttcaatggtg
     3841 accctccaat caaaaacata tcttcatcta tactttaaca taaataaacc gataaaatcc
     3901 acatcaaaat tatacacaca ataaaaaaga tatatatatt taaacaaaac tatttacttt
     3961 aagttgtaag cggtgttatc attaagtgga aacaggaggt aaaaaaatta tgacaactat
     4021 gaaaaagcta acttttccag atgatttttg gtggggttcc gcttggtcag ctgagcaagc
     4081 agaaggaaaa ggagaaacag gaaaagcaga gactatctgg gatcgctggt ttaaagaaca
     4141 accttaccgg ttttatcaac gaatcgattc ctctattaca accgatcaca tccatcaata
     4201 taaagaagat atagaactaa tgaaaaaaac tgggcataac tcctttcgcg tttctatttc
     4261 ttgggcacga atgtttccaa atggcggtac cggaaaagta aatcccaaag ccatttcttt
     4321 ctatcgaaaa ttattttccg aaatgaataa taacggaatc aaagtatttg ccaacttata
     4381 tcattttgac atgcctgcta ctcttcaaga taaaggagga tgggaatcac gtgaagtagt
     4441 tgatgcttat gttcattacg ctgaaacttg ttttaaagaa tttggtgact tagtttgtca
     4501 ttggtttacc ttcaatgaac cacttgggcc tattttaggt acttatcttg aagattttca
     4561 ttatccaaac ctcattgact ttaaacgcgg agcgcaagca gcattcttca ctatacttgc
     4621 acacgcaaaa gcaatacaag tatttaaaga actagctctt cctagtaaaa taggcgttat
     4681 tctaaatttg agtcctactt ttcctcgtag tcaaaatcct gctgatgtga aagcagcaga
     4741 aatcgctgat ttattttata cgcggagctt tttagatcct atggttaaag ggaatttttc
     4801 taaaaagctt atcctgttat tacaagaata taatcagctt cctgaagatt atacgaaaga
     4861 agatcttgta tgtatctacg aaaataccgc tcaaattctt ggactaaact attacgagcc
     4921 acgtcgcgtc aaatctcgtt cttctatggt ggatacatct agtccatttt taccagagtg
     4981 gtttttcgaa ccacatatca tgcctggacg acgaatgaac gaataccggg gatgggaaat
     5041 ttacgaaaaa ggaatttatg atttgtgcat ggatatcaag aaccattatg gaaacatcga
     5101 agcatttatc tcggagaacg gaatgggcgt tgcggatgaa gaacgtttca tggatgatac
     5161 agggcaaata attgatgatt atcggattga gtatatcaaa gatcatcttg cttacttatg
     5221 gaaggcaata aatgaaggat gtaacattaa ggggtatcat ctttggacat ttattgattg
     5281 ctggtcatgg atcaacgcct ataaaaaccg atatgggctg gtttcactcg accttactac
     5341 tcaaaaaaga acaattaaaa aaagtggcga attttacaag caattaagtg atacaaatgg
     5401 atttagatac gatgtagact tgttagttga ataatattct aaaattccac ttgaagtgaa
     5461 cacattagaa aacaagaata atgagaaata ccaataacaa atactttttc ttcatacttc
     5521 atttatactt gtcagtataa gagactgtga caaatgtagt gatcctcaga agttaggtct
     5581 ggaaatctaa cttgagaaga tttttttaaa ctacgtccag tcttttttat ttgtttaaaa
     5641 ttgaagttga caagtcttca ttaaaaattt agaatattca caagtttgac gacaattatg
     5701 aaaatttatg tcgtgctaaa ggagttttgt tattggatga aaagttagat ataaaaactg
     5761 cgatcaaaga cacgttacct accgtttttg gttatatcgg tattggactt gcatttggta
     5821 tcgttgggaa agctgccggg tttcatccgc tggtcgtcac gttgatgtcc ctgctgatct
     5881 atgctggttc tgcccaattt atcacggtca gcatgcttgc tagccatagt ccactgctct
     5941 ccatcgttct ctcgaccttt ctagtcaatt cccgaatgat tctgatgagt atgacgattg
     6001 ctccttattt caagaaaaat cggctgcttc aaaatctgct gattggcaca ttgctgacag
     6061 atgaaagttt cgcactggga atgaataaat tgaactatac tggacaaaag ttaaactttc
     6121 gttggatgaa tacagctaat tggatttctt atttgacgtg ggtgggttct tctcttgttg
     6181 gcgcactttt agggaatttt attactgatc cgaaaaagtt tggtttggat ttcgccatcg
     6241 tcgctatgtt tatcgggtta ctttatcttc aagtcatttc tgatagaaac acctctaaac
     6301 gattacagtt gatcctgatt ggtctcactt tgattctggt atatgtcgga ctgattttta
     6361 tcccaagtaa cttagtgatt ttagttgtaa cactgattgg gtgcggattg ggggtgtgga
     6421 tcaaacatgc cttcttttga gtttacattg ctgactattt taggttgtac tttagcaact
     6481 tggctttcta ggatcctacc atttgtctta ctgaaaaaat tcgatttgcc gcaaccgttg
     6541 cttgagtatt taagttttgt cccgatcgtc attatgtctg ctttatggtt cagcagtcta
     6601 tttacccaaa atatcggaca tctgccgcag atcaatctcg aaaatgcttt ggcttcagtt
     6661 cctacacttt tagctgctat tctttcaaaa agcttattgg tgatcgtctt ggtaggaatt
     6721 ctttcccttt cgttgattcg cttattataa ataaaaaaac aagagcttat ggcaaattac
     6781 tgtcatgcgc tcttgttttt atcaaagtca aataagaaaa aaatcggcgt attcttgctt
     6841 atccgttgaa cggaccacta acaacgactt ttcctaatat atgtcctgtt tctacgtctt
     6901 tggttgcttg tttcaagttc ttcgcattca tcccatctga atagaccttg ctcaatgtac
     6961 tgcggataat cccttcattt gctaaagcag caatgtgcgc taaagcttct ccttgcgttt
     7021 cgatttcata gttatagtct gttttagcga acatatactc ccagtcgaag ctgatagatt
     7081 ggtttttcca aacacctata tctaacggct ctttgatccc tacgatcgtt cccacatgtc
     7141 caaatggtcg gacaaggtgt ttgatttgat caaagtagcg ggtaatatcg aataatacag
     7201 cgacatactc tacttgatcg attcctaatt cacgaaaact tttacttaag tcgtgatgat
     7261 aatcgattgg atagtctaca cctgtttttt tcagccattc aaaattcttc ggacttgcag
     7321 tcgcgtagac ttctaggcca gcccaatgcg ctagttgatt caaaatcgaa ccgacgcccc
     7381 cagctccgtt gatcactaag atttttttcc cgcggttggc attttcttct ggagtcagac
     7441 caaatttttc aaataacaat tcataagcgg tcaatgaggt caacggcaag gctgcggctt
     7501 cttcatctga aagatttttt ggtgcaagag cgacaatacg ttcgtcaacc agttggtatt
     7561 cttgattgct tcctgctctt gtcgttgtac ctgcgtaaaa aacgcgatcg ccgacagaaa
     7621 atttttgcac ttcatcacct acagcaacaa ctttcccgac tccatcaaac cctaagatac
     7681 gtaaagcatt tcttatcccg ttatcttggc gaagtttcgt atcgacagga ttaacagata
     7741 ctgcgttgac ttttaccaga agatcccgtg aactgggtac aggtatctgt tctttttcat
     7801 caataaagct ttttggatca tcgatcgaca acccttcata aaaaccgact accttcatta
     7861 attccttttc tgacatacga attcctcctt agcttcatca agttggagca tccatacagt
     7921 tttcatttca atatatatag tatgctattt gttccacttt tattcatcat aattattctc
     7981 ttacttaaag taaactattt gcactttttt ggttaaaaaa gaggttgcga caagactttt
     8041 gtcacaacct cttttaaacg gtgttcaatc aactgaccct cggtattcgc tcaaaaaacg
     8101 acaaaatatg aggagctatt tttgtttggc tgttcttatt acttgatgca ggacagcttt
     8161 ttcacgacct ctaattcacg gtgttcaagc aagaaatcat tgttcactga aaagcgttgg
     8221 atgttctttt tcaaaggtgt ctaagatctc tcgtacttca tcagtagatt tcgtattcat
     8281 caactgaact cgcaagtcat ttgctcctgg aaatcctttg atatagattt taaagaaacg
     8341 atgtagtcct gtgatggaac gcgggatgat ttctgaataa tgatcttgca gatctagttg
     8401 cagacgaagt agtccgatca gttctttttc tgtatgttcc ctcggttctt tttcaaatgc
     8461 ataaggattc ttgaaaatcc cccggccaat catgatgcca tctacgccat atttttcggc
     8521 caattcttct cctgtctggc gatctgggat atctccgttg atcgtgatca gtgtttgcgg
     8581 agcgatttgg tcacgtaaag caatgatttt agggatgact tcccaatgtg catctacttt
     8641 actcatctct ttacgcgtac gcaaatggac agaaagattg gctatatctt gatgaagtag
     8701 gtgactgatc cattcttcca tctcagacat ttccgtatag ccgatacgcg tcttgacgct
     8761 gacaggcaaa ccgccagctt tagccgcttc aatcaattgt gccgctactt ctgggcgaag
     8821 gatcaagcca ctgccttttc cacgttctgc tacattaggg actgggcagc ccatgttgat
     8881 atcgattcct tggaagccca tttctgccat agcaatgctc atttctctaa aaaattctgg
     8941 cttgtctccc caaatatggg caaccatcgg ctgttcatct tctgtaaagg tcaatcttcc
     9001 tcgtacactg tcttttccat ctggatgaca gaaactgtct gagtttgtaa actcagtaaa
     9061 gaagacatcc ggtgctgctg catgctttac gacatggcga aatacaacat ctgttacatc
     9121 ttccattggt gctaaaacaa aaaaaggctt tggcagctct ttccaaaaat tagtagtcat
     9181 ttatttgtta ctctccattc tatcaagcag tgaagtcgca atctttctca aaaacattgt
     9241 cattatacta ggaaacaaaa aaagaagcaa agtgaacagc ttaaaagatg tttctgattg
     9301 agcaaaaaaa gactgtgaca aaaatcttat cacaattcct tttccggaat aaatggtgtt
     9361 caaaagcttg catctgcggt aataagtcca accaaacgaa aaataggagg agctattttt
     9421 gtttggctgt tcttattact tgatgcagaa cagctttttc acaacctctc cactaaacgg
     9481 tgttcaatca gctgaccctc ggtattagct caacaaacga caaaatatga ggaactattt
     9541 tcccgtttgt ttctctaata ctcaggtcaa aacgctgatt tcacaacctc ttttctaact
     9601 tctcaatcct cttcctttat tgatcaaata ccaagtaacg gcgtacaaaa caacgatgaa
     9661 tagcacaagt acgatcatcg atgttgctac tggcacatcg attgttccaa ggaatccgta
     9721 acggaaaccg gagatcatat aaacgatcgg attgactttc gaaacagctt gccaaaatgg
     9781 cggcaacata gagatggcat agaaaacgcc acctagataa gttaacggct gcaagacaaa
     9841 tgtaggcacg atcgatacat cgtcataaga ctgagcaaaa atcccgttga gcagtcctgc
     9901 taacgaaaac aagattgccg tcatcaataa tgtaatgatg accatgaacc atgaatacac
     9961 atgaagtggt acaaagaaca aggaaatgat cgttacgaga gagccgacga gaatactgcg
    10021 acccactccg ccaatcacaa agccccaaat gatcacatgc gtaggaacag gcgctaccaa
    10081 gatttcttcg atgtttttct ggaatttttg agagaagaag gacgaagaaa cgttggcata
    10141 cgaactagtg atagcagaca tcataatcaa accaggcacg atgaattcca tatatgaaaa
    10201 accacccatg tcaccgatcc gaccaccgat catttttccg aaaataacaa agtacaagga
    10261 tgtcgtgatc acaggcggaa ccagtgtttg gacccagata cgcaaatagc ggttcgtttc
    10321 cttgatagct aaacttttta atgcagtaaa ataaagatta aacatttttc tcctccgtat
    10381 gattattttc ttctgtgatt ttcaaaaaca gttcttctaa acgattcgac ttattcctca
    10441 tagaaagaac tttgattcct tgtcttgtca gctgatcgaa gattccgttc accccttgat
    10501 ttcgttcaac ttcgacagcg attgtctgtt catcttcaaa tgtacaagcg tatccttcaa
    10561 tcacaggttt ggtctcatat ggcgctaaat caaagataaa tgtttcaaat tgcagtttgg
    10621 atagaagctc tttcatacta gtattctcga tcagttcacc tgattggatg atcccgatgt
    10681 tgcgacaaag catttcagct tcttctaaat aatgcgtcgt caaaatgatc gttgtaccat
    10741 tttcgttcaa ttctcggaga aactcccaca tctcgcgtct caattcaata tcgactcctg
    10801 ctgttggttc atccaagatc agcaaacgtg gttcatgcat caatgcccta gcaatcatca
    10861 gtcttcgctt cattcccccg gaaagcatac gtgcacggac atttctcttt tcccaaagat
    10921 tggactgctt caaatatttt tcgctgcgtt tcatcgcttc tttgcgagag acaccgtagt
    10981 agcctgcttg attcacgacg atttgctgga ccgtttcaaa gggattgaaa ttgaactctt
    11041 gcgggactaa tccaatctgt tgctttgctc ggactagatc cgtatccaga tcataaccga
    11101 agacattcac ttttccagat gttttattga ctaaagaagt aatgattccg atcgttgtag
    11161 acttcccagc tccatttggt cctaatagcg catagaaatc tccttctttc acggaaagat
    11221 cgattcctcg caatgcttcg accccacttc cataaacctt tttcaaatta tttatttcaa
    11281 gcgcatttgc catttatttc ctatctcctt atagacatga ttctttattg tatcatggaa
    11341 tacagcagat gaaaaaacaa cttggttcca gcgaaaaatc aggttgctgg gctcaggaaa
    11401 atctcctaat gaaagacatc aatcggacga agtcagacaa ccttttcaga aaaaacaaat
    11461 cgaagataaa ggattactat tcatatgtgc aacaaaagga gcgtgagaca aaagtaaaga
    11521 acacttttgt ctcacgctct aaacacgtat aaacggcgga agcaaaagcc aagatttttg
    11581 atccaataaa tactttcgat cacttcgcct atcccaatac atgccactgc cgcttttcat
    11641 caaataacgc ggcttccgtt tttgcggttt ctactaaagt ctctgggtaa tccaaaactt
    11701 tcagcaaggc aatcgcattt cgggtccttg aaggtccttc ttttaatttg aaatcaaaac
    11761 tgattccttt tccttctgtt acttgttctt caaagtggac attctcacaa taattcttta
    11821 aaatctctgt caattcgata tcatgtgtgg cgacaaatgc taaagatgga tactccgcaa
    11881 gccaccgaac gactgaagcg gaagaagcaa tccgttcgat cgtatttgtc ccttttaaaa
    11941 tctcatctac gaagcagtag cagcgttctt tggaagccac ttggtccaac aaacgtttga
    12001 tcgatttgat ttctgaaaca aaataactgt cgccttcaaa taaatcatct tcgattgcca
    12061 tggaagtcaa cacatgtcca ggttgcaggg taaactgttc tgcaatcgct gtttggatcg
    12121 tctgtgctag aatacagctg atggctatac ttttgacata cgtagatttt cctgatgcat
    12181 tggaacccgt gactaatgta ttcttctgcc agttgaccgg attcactacc gcttctttta
    12241 acaatggatg atagatatct ttcgctgtca caccgccctc agtaaactct ggtcggcaag
    12301 tgatgggcat ataagtacgg taattcaata ttgcagcggc tacttctaat tctcccagca
    12361 attcccacag cttgatggct tcagttgaat gcttttctaa tttagtgaga acgaaatgat
    12421 aggagataaa tggcagcatg aacatgatgt tgacatattc aaaaatcaac tctgcttcac
    12481 tcccactctt catacgaaat gaaaaaccta atttagggat gtctcttaat ggtttagttt
    12541 gctgtttcag ctcttcttga agtggtgtcg gcagttttgc tagctgattg gcggctgaaa
    12601 tcgttgaaac caaataactc atgctattca gctctgtttc tagtgtttgt ttcttcacta
    12661 gataatagac cgtattgaat actaacccac cgattgccaa atagattcct agtacttgtc
    12721 ctaaaagaag caaacccaaa cccaccagcg gaagcaagcc aagaaaaata aataaaccta
    12781 cgcgtccaat ctttttactt tttccatccg ctagatattg ttttgcaaaa ttatgatctt
    12841 tttttcctaa acgagcgaat tgatattgga ttttttctct cagttgagga ttttcctgat
    12901 aaaaagcaat cagtttttct aactgctgat cttcaccaaa atcaaagctg cgtaaacgtt
    12961 gatacaacgc ttctgaacct acactagaat aggtactgtt gattccttcg aatacttcga
    13021 acatatccag gtcataccaa gtaagatcat cgatttcgct atcccatgaa cggaacttct
    13081 tctccgtgag ccatgcttcc ttcaagcttt cttctttatc aaatctcggt tgataaggaa
    13141 ttttttccca tttgctacga actgtttcct ttaatttcaa acgtgaaaaa atgtctaaag
    13201 caactaataa aataacagct gcaataataa ttaaaacgat aaattctctt tgacccatat
    13261 ccctctccgt tcctttattt gttagcttta gtatagcact gttgcgctat ttgaaaagag
    13321 ggtaaaaaaa cacctttcaa aataaaaggt gtttttttct tgtatattac atatttatct
    13381 ctcatttatc aaaattgaca aataatcata gggaggaatt gttgttaatt cttgtttatt
    13441 ctccagtgca ttgacaactg tcccaaggga aaggatagcc tcaggtacta ttttttcttg
    13501 ttctgttgga ttagctaagc gaagaacaaa tccttgttca tgaaaataag aaggataaat
    13561 cgatgataca attaagtctt caggtaattc aatcaatgaa aatttacgag gaacatcaag
    13621 ttcattttgt aatggccaaa tcttgttatc taacctgtga ataaatacat tcagtttctg
    13681 cttttgataa gaaatagatt gttctaaccg tttttctgcc tgttttgcca ctttgaactc
    13741 attaaatctt ccttcttcta cattaaatcc aaaagtgact ttatactcac ctatctcttg
    13801 agctagcggt gtttccatca tgacatgccc ttcatttgtc gtatctcctg aagctcgccc
    13861 aggtcgccaa gcaagattag gttttccaag ttcccctgtt gttgaaaata aggttatgat
    13921 taattgattt tctatacgtt catactcttt taaaccatct gcataaaacg taaaataatg
    13981 atcattttct gccacagaga cactcttatc gaatatttct aaattaacag gtttctctac
    14041 catagtttct gaccaattcg gaggaatgac atcaggggta ttttcaataa atccgttttg
    14101 aatttgtgca attgattttt cagatttatc taaaacatta attgctaagc gcatacgatg
    14161 cgagtaaaca gtattatgaa ttttgagtgt gccttcaacg atttgacttt ctcccagact
    14221 gaacgatatt tcataagata tttcttccat ttcgggtttt tcagataaac gatcttttaa
    14281 atctttagga agttgagctt ttccgtaaac aactaatgtt tcttgtaaag aatctttata
    14341 tactttagct gtttctaatt ttagagatag ttctgtatct ccttctaatg gtgaataatc
    14401 atacgtatcg ccatcatttg ctgaatcgat caaatgaacg aaatcaagat attgtctatc
    14461 tccaactatc aaattcaatt gcccatcttt aaaagaaagt ttatatttat tgttacttat
    14521 ctcagttttg tctatctcga ttgctgtttc taatttttca ttatttttct caaattcaac
    14581 aacagtgtat cctagtgctg gcacatttac atttattttt atgtcaagtt cataataagc
    14641 tggttcatca aaatattcga aacctgtagc aatcatccga taagcatttt tccgttctgg
    14701 aaaataaact tctttttcaa tgatattata gggactacct ttgaagtgta ttttctttga
    14761 atcagctact acatgcacga ttttcgatcc actataattc tcagtcgttg tgttgaaaac
    14821 aaggacttca ttcgatttta gctttaatgc atcagccatc cgttttacaa taaggttttc
    14881 gattccgtca gctatctcat tagcttcttt gattcgatgg aaaatatctt ccgcaacatt
    14941 atctgaaaca cagccaccaa tactatcatg agcctggttt tgcaaaatct ttttccataa
    15001 ccgacgaagt aggcctttac tgatatggat accatttctt tctgcaatca gcatgagcgg
    15061 ctcaactcgt tttagaattt tttgttccaa tttaaaattt tctattttca tttttgttct
    15121 tactgagcca atagaacgat gaacacgaga gtaaaccggc tgacgaagtt cacctttata
    15181 ttcaggtaat tcttttttgt ttctgataac ttctacataa gaaggataat ctgaaataat
    15241 attgtggaat ttactgtatt tattcaactt ctctacgact tgatcaaaat ttaaaatcat
    15301 tgacttttga tctacacccg aaggtactaa aatatctttg ttttttcctt gcatatgaag
    15361 catttcaact gcttcatcca acttttcttt tgcaaattca ggaatatctt taattgcgtc
    15421 taaggtcatc attccagtca tataaccaaa tggaaaattc atggcataaa caaatttatc
    15481 tcccaatcct ttccagataa aataaggaga tggaaccatc gtttcaaagt ttaatcctcg
    15541 ccaacacatg aaattatcga tttctacttg atgcaaaagt gtaggcaaat tcgcattgaa
    15601 cccaaacgtg tcaggcaaat accctaccat cattggatct ccatatttaa ggatcgtatc
    15661 actgattcca atcattaagt ttcttaaaat tgattcagca tccactaata aggcatctgt
    15721 ttgtgcatac cacggtccca cgaatagttg tcctgttttc atcagtcgtt ggataacctt
    15781 aagcttttgc ggattgatct ccaaatactc atcgacgatt gaagtttgtc catctaaaca
    15841 aaagtttact ttagggttcc tttccaattc atctagaact tcagtaaaga cctgatcaga
    15901 caatacgatg gcatcttcat ttgtaaaata ccattctctg tcccagtgcg tgtgttgaat
    15961 tatatgtgca tttaccatat cagattctcc tactttcctt cttctactga atttacttgt
    16021 tgttcttttt tatacttgat ggattttagt gcattgacca ttaacgctgt aaccactccc
    16081 ccaataacaa ttgatactaa atataaaggt atattcgtaa caaaaggaat tgccataaag
    16141 gttgaaagca ttgtttcttg agtaacacca aaaaatccat ttagaccaca tgctactgca
    16201 gtgcccacca cgatgcttgg tattactcgt aaaggatctt tcacggcaaa cgggatcgtc
    16261 ccttcagtaa tccccacaag gcccataatc atagctgtct ttgcattatc tcgttccgat
    16321 tcatcataaa gttttttgtt taaaattgtt gctaaaaaca ttccaaatgg cggaatagca
    16381 attcctaata acatggctgt actaggtcca aagcttccgc ttgaaaaaga agcaacccca
    16441 aaagcatatg cgattttatt gataggtcct cccatatcaa acgccatcat tcctcctaaa
    16501 atagccgaca tcaaagcagc atttgctcct gacatgcctt ctagccaatt agtcagtcct
    16561 gttgtcattg cttgaatagg cgtagctagt acataccata atatgattcc aacaactaat
    16621 gtagtaacaa aaggaatcag cataattgat tttagactaa taacttgcat cggtactttt
    16681 acttttttca atgcattagc taaatagcca caagctaatc cagcaaccat tcctccaata
    16741 aatcccatac caagattttg acacatcatc ccagtgataa aagctggtgc tagaccggga
    16801 cggtcagcaa ttgaatatgc gatataggct gaaatcatcg gtacgataaa ctgtaatccc
    16861 gtctgaccaa tcattcgcag gactccccag aatgtatccg tctggtccca aacacctgtg
    16921 ccaccaaaaa tgactgcaat agaagttagt aaccctgctg ctacgacaag cgggatcata
    16981 taggaaaccc cactttggaa atgatttaga atttctcttc cttgtttctt gaattccatt
    17041 agtaactccc ccacgatata ttttttattt gttaattaat gcaattgctt catcaatcgt
    17101 ctcttcacca tcttttacta cctctgaggt ccctactgtt aaaatcggtt taccgtcaaa
    17161 gcgacctcgt tctgaaacat ttgtatctac tgcaaatatg acaacatctg cctcggaaac
    17221 atccgaaatt gaaagtttgt tttctatccc catagccccc tgtgtttcta ctttaatatc
    17281 taaccctttc ttcttcgcaa acttttctaa attagctttt gccatataag tatgggcaat
    17341 ccctgcgata catgctgtta ctgctactat ttttgtcata tgaaattcct ccttttaaaa
    17401 tgatagagct tttaccaatt cttttttatt tctcgaatct tttattcttt taacaaactc
    17461 gtcatccatc aatcttccag ataattctgc taaaatacgt aaatgattag tacctttatc
    17521 agtgtctttt attgctaaca aaaagacgag atcaactggt tgatcatcta atgcgcccca
    17581 ttcaacagac cgctttaact tggctacagc cactgttgat tcaattactg ctaggctttt
    17641 gccatgagga atggcgatat ttttcccaat tcctgtggtc atatggtttt ctctgtcaaa
    17701 tacgtctttt tgaaattgtt tgtggtcaga cacaaaccca ttagtctcta acaatttcga
    17761 taatttttcg actacacttt ttttatcaga tgcatttaaa tctatcacta tattatttat
    17821 attgattttt tcactgatca tttataagcc ttctttcaat atataaacta atttttcaaa
    17881 cgacgaactc gtttttaaat ttgctaataa ctcatgattc gaagcgattt cattgaatgt
    17941 ttcataaatt ttattcaatt caggttttac tttttcatta agtgcaaaaa agaaaacaga
    18001 atgaacaagt gtcccatccc aatcaattcc ttctggatta acgtagacac agattactgg
    18061 cttttttata aaatctactt cgatatgcgg catagcaaaa tcattcatcg ctgttgaaga
    18121 tagtttttct cttttgattg ctccctctag tatcccttct ttcgcgtaat tttcttttac
    18181 caattttttg gtaattatat ttaaaacgtt cacttgatta tcctttattt ggtgggtaaa
    18241 aaagcaaaat cgtttatcta ataaatttag aaaagcatct tctgtttgtt catttaactg
    18301 tagatactgt tttacttttc tgacatctgc ccctaacatc aaaggactaa caacaataac
    18361 aggaaaattt gtattttcaa tcggaatcgt gcttataaca agttcatcag tgacttttct
    18421 atcttgtagt tcattgatgc ttagtacatc ttctatctca accatatctg caaaaccgtt
    18481 atttattctt tgctctaata attttgatgt tccatatccg ctactacaaa ctaatatcac
    18541 tttcgttttg tcaggtttgt aacgatccaa aagtgattga atatggagtg ttacgtaagc
    18601 gatttcatct tcattaaaat gtatacagaa tctctcttcg atttcctcta ataaatctac
    18661 acttatcata aatgaccttt taaaagaata tttgattttg tccgtataag ggttatagat
    18721 atttactccc aatttgagtc ttttaactgc tgcattgaga tgtaatacta gattattgat
    18781 caattcttca tcaggataaa attcatttaa tttaattctt actaactctt gtagctcaac
    18841 acccatatct aaataggaaa gcttcgtttg atctaagtcg tgattcatag catttttttc
    18901 aatcgaagta atgtgaatgt caacataagc ttgttcatct attggcacaa taattgaaaa
    18961 tgctttttca atcattttga ttaaaataag tgtattcttt gagatttctt cttcagaatt
    19021 attactacgt gataagaaat tgcttgtttc aatcctttct atcgcaatag caaggtggat
    19081 agctaaagtt tgaaattcat aatctgtaaa tttcaattgt gtcttattca tgtatcgatt
    19141 aagcaattcg gctacttttt tcaatatgtc cttatttaag aaattattcg cttcaccaat
    19201 gaaattgata ttcaagtcta agagatctgt tttactgtta gatgctgtaa agccacccca
    19261 ataatagttc acaacttctg acatcaacct tcttttactt ttttcatcag ctttgatgcg
    19321 cacgccattt cgatctgaaa ctaaggacat cccatgcttg tttagtcttt ttctaacatc
    19381 tttcattgtg ctttctaccg tcgacctgct cacgtataat tcgtcagcaa gattttgaat
    19441 ctttagagaa tcattagtat tcaataattt agcaaacaca taagttagac ggtcctccgg
    19501 agagtctgcg atattgattt tatgactgga taataaattt cgaagtgttt ctttttttcc
    19561 aataagcgaa acgccttttt taggttgaac caacatttgt acatctggca ttttttggat
    19621 tatttctttc aaaattaaaa tatctttgct aattgttttc gtagatacat ataattgttc
    19681 tgataattct ttgtaagttg tgtgctcatt ttcaatcaaa aagtatagta ttttcatttg
    19741 ccgttcgcta aattcgatgc ccattatctt cacctcctga atacagctta agtgtacaat
    19801 tattatctct attttcatac agaagaaagt tcttgat
//
LOCUS       DARBEC010000054        19778 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.054, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000054 DARBEC010000000
VERSION     DARBEC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.054"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            99..293
                     /locus_tag="SCL80_001778"
     CDS             99..293
                     /locus_tag="SCL80_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107645.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            562..861
                     /locus_tag="SCL80_001779"
     CDS             562..861
                     /locus_tag="SCL80_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107646.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(958..1392)
                     /locus_tag="SCL80_001780"
     CDS             complement(958..1392)
                     /locus_tag="SCL80_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1107647.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1624..2334)
                     /locus_tag="SCL80_001781"
     CDS             complement(1624..2334)
                     /locus_tag="SCL80_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEG1107648.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2324..3184)
                     /locus_tag="SCL80_001782"
     CDS             complement(2324..3184)
                     /locus_tag="SCL80_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEG1107649.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3189..3827)
                     /locus_tag="SCL80_001783"
     CDS             complement(3189..3827)
                     /locus_tag="SCL80_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEG1107650.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3842..4141)
                     /locus_tag="SCL80_001784"
     CDS             complement(3842..4141)
                     /locus_tag="SCL80_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1107651.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4184..5668)
                     /locus_tag="SCL80_001785"
     CDS             complement(4184..5668)
                     /locus_tag="SCL80_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEG1107652.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5688..6149)
                     /locus_tag="SCL80_001786"
     CDS             complement(5688..6149)
                     /locus_tag="SCL80_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1107653.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCL80_001787"
     CDS             complement(6167..7234)
                     /gene="ulaG"
                     /locus_tag="SCL80_001787"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEG1107654.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7452..8222
                     /locus_tag="SCL80_001788"
     CDS             7452..8222
                     /locus_tag="SCL80_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1107655.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8495..9322)
                     /locus_tag="SCL80_001789"
     CDS             complement(8495..9322)
                     /locus_tag="SCL80_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107656.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9890..11125
                     /locus_tag="SCL80_001790"
     CDS             9890..11125
                     /locus_tag="SCL80_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1107657.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12124..12384)
                     /locus_tag="SCL80_001791"
     CDS             complement(12124..12384)
                     /locus_tag="SCL80_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEG1107658.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12411..13712)
                     /locus_tag="SCL80_001792"
     CDS             complement(12411..13712)
                     /locus_tag="SCL80_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1107659.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14004..14462)
                     /locus_tag="SCL80_001793"
     CDS             complement(14004..14462)
                     /locus_tag="SCL80_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1107660.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14475..15806)
                     /locus_tag="SCL80_001794"
     CDS             complement(14475..15806)
                     /locus_tag="SCL80_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEG1107661.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15817..17259)
                     /locus_tag="SCL80_001795"
     CDS             complement(15817..17259)
                     /locus_tag="SCL80_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1107662.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17246..18010)
                     /locus_tag="SCL80_001796"
     CDS             complement(17246..18010)
                     /locus_tag="SCL80_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEG1107663.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18114..18989)
                     /locus_tag="SCL80_001797"
     CDS             complement(18114..18989)
                     /locus_tag="SCL80_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1107664.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19244..19328)
                     /locus_tag="SCL80_001798"
     tRNA            complement(19244..19328)
                     /locus_tag="SCL80_001798"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19292..19294),aa:Leu,seq:caa)
     gene            complement(19346..19417)
                     /locus_tag="SCL80_001799"
     tRNA            complement(19346..19417)
                     /locus_tag="SCL80_001799"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19383..19385),aa:Gln,seq:ttg)
     gene            complement(19427..19499)
                     /locus_tag="SCL80_001800"
     tRNA            complement(19427..19499)
                     /locus_tag="SCL80_001800"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19464..19466),aa:His,seq:gtg)
     gene            complement(19561..19645)
                     /locus_tag="SCL80_001801"
     tRNA            complement(19561..19645)
                     /locus_tag="SCL80_001801"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19609..19611),aa:Leu,seq:caa)
     gene            complement(19666..19736)
                     /locus_tag="SCL80_001802"
     tRNA            complement(19666..19736)
                     /locus_tag="SCL80_001802"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19702..19704),aa:Cys,seq:gca)
BASE COUNT         6276 a         4090 c         3077 g         6335 t
ORIGIN      
        1 tgattctttt tatttatcat aaagatatag atacataatc attttcactt acatatattt
       61 gaatattaac taaaaacagc aagaaagaag aggatcaaat gtattatgta gaagtaaaaa
      121 caaaaggcgt caaaaacaaa caatatgtga aaggtatcag taacgaatat cctttacttg
      181 gttcatggaa agaagctgct cctttttcca aaccatgtgc cataaaaata aagaacgagc
      241 tagaaaaaga actgacttgt ggtaaagctg ttgtagatat catcgaaaag taaatccaat
      301 gtatagaaag gaagatcaca catgaaaagc gacgtacaaa ttgctaagat tatagaatta
      361 gaagaagaag acgcagtgtt gacaattgaa aaaaaataaa tgtttgtata ccatatgatt
      421 atttcaatat gtatcctgga attggagatc tcgtagagct atatcaagat gaatcaaata
      481 tacttgtcat tcatcatgaa atatagagac ttataaaatt gtttattttt ataatgtatc
      541 agtatagaaa ggaaataaaa catggattat actgttgaat ttgacagtga actacttaag
      601 caatttccac aagaagaaca tttcatgtat aaagaagcaa tttcccacaa agtgacagag
      661 tatttggagc ataatttttt tcgtataaag ccagtgcgta ctcgttcggc aaaaggaata
      721 tacgagatga aagtcaaaat taataaaaaa aattatcgca tagcatttgc tttaaaaaaa
      781 aatcatgtgt tcatttttta tattagttcg aatttgcaaa aaattgtatt tgataaagag
      841 gtgagtaaaa aaataaaata gctgagcatt ggaggataga ttgagtgata ctaagtctat
      901 cctcttgttt ttctaaagaa ctaagctaga attttttctt ttgtacgtta ttatatgtta
      961 tttgtcatta ccaaaaagca ccttttttct tctagtatct atgacataag aaataccacc
     1021 catttccttt acttgaacaa atcctactga ttcataaaat ttttttgttt tctcagtatc
     1081 atctgtcaat aattcgattt ggactggtcc ataactagtg atcttctcca aagcttcatt
     1141 caaaagcact ttgcctattc cttggcgctg atacctcgga tgaatgagga tatcttgaat
     1201 aaatgcgatc gtcgcaaaat cagtgatcca acgaacgatt ccgataatct tcccttcccc
     1261 attacgcttt atcagagatt cagattgttc aaatgctcta gctaaccttg cagtgtcttt
     1321 tgtgtaattc gtccaaccaa cgctcttata taattcagaa atctcatcga tcataaggga
     1381 atcaatattc atttttttct tcctttctta tacaattctc cctctaataa tacagttctt
     1441 tgactattta atctatcaca tataactctc ctgtaaagat tctctcgatt ccttaaaatt
     1501 cctctttcct tttagaatgc atactttttg ataaataaaa aggttgtgat aaattttctt
     1561 cacaacctct gtttctctct atcaagtgtt atcttttaat tttctaccct taccctagca
     1621 tctctattgc ccgtagtatg catactttcc atgtttgcga aaataatgtt tatctagtaa
     1681 atattgcggg atttcacata tattggggtt gatcagctcg ttttctttgg ccatcagaca
     1741 aatttcatct aagatcaaac tgttttctac tgctttcata ggtgaatccc cccatgtgaa
     1801 cggtccatgt ccatagacaa gtactccagg gacttcgttt ggatctaatt ttctttcctt
     1861 aaacgtctcg acaatcacat tccctgtatg aacttcgtaa gcttctctta cttcttcttt
     1921 cgttaattgg cgtgtacaag gaaccttacc ataaaaagca tcagcatgag tcgtgccata
     1981 agctggcaaa tcgcgtcctg cttgcgccca catgacggag tgcgtggaat gagtatgagt
     2041 aatagcagtt atatcttcaa aagtttgata caaaacaaca tgtgtgggga gatctgaaga
     2101 tggttttaag ctatcttctt ctattatatt tcccgacaaa tctgttacca ccatttgatc
     2161 tgcttgcatg cattcatatc tgacaccgct tggcttaatg acgatgactc ctagctcacg
     2221 gtccacttca ctcacattgc cccaagtcaa tttgactaat ccgtatcgcg gcaatgccag
     2281 atttgcttca aggacacgtt gcttcattgc tgcaattttt tcttcacaag acatcatagc
     2341 cagcctcctt caatttagaa cttaagtagt cttttgcctg ctggatttct cttcgaaagt
     2401 cttggctttt ttcactccac atttcaatca gaaatgttcc atcataatct agacgtttca
     2461 aatgtcttaa taatccaaga aaatcaacac aaccgctacc aaaaggaaca tcacgaaact
     2521 ggcctgaata ttcttttgtt acagggtaag tatcttttaa gtggatagca gtgatataat
     2581 cgatcccttt ctcaagttct cgagccggat tattttccgg ccatgcggac aaattgccta
     2641 ggtctggata tacttgtaga aaaggtgaat gaatctgttc tttgatttct aaaaattttg
     2701 aaatggagtt cataaatgga tcatccatga tttccacgga aagaatgatg ccatatttag
     2761 aagcttcctt tacgctttct tttattcctt cgataaagtc ttctctggaa ttcattgatt
     2821 tctcttcata ataaacatcg tatccagcta tttggattac tttgacgctt agattatgcg
     2881 ccaaatggat tgcttttttc attagttgtt tcgctgcttt tttcttttct ggatcagcag
     2941 aaccaaacgg aaatcgccga tgcgcactta aacaaatcga atggatacgt acaccggagc
     3001 tgaacatctc ttctctcagc tcagctatct tttcttcaga ccaatctaat cgtgctaacc
     3061 gctcatccgt ctcatcaatc gacatctcga taaaatcgaa gtccatttct tttacaaggg
     3121 aaaaacgttc tttccatgaa atatctttgg gtaacgcttt ttcataaatc ccaatttgag
     3181 ccatttcctt acccccagat acgaatgatc tcatctttga agttttgtgc ggccttcatc
     3241 ggatcatttg ctgctgtaat accacgacca gtgataaagg tatacacatc gatcccttca
     3301 aataagtgta atgtgtgtgg atttaatcca ccagttacag aaacattaaa gcctagttca
     3361 atcagttttt tgatcttcga taaatctttt tcaccccaag tttcaccaga taacagcgca
     3421 tcacggcttt gatggtagat tacctgccgg ataccgatgt tatgccaatc catggcttgt
     3481 tcaaaagtcc aattgccgta gagttctact tgcaattcac ctatttcttt ttgtgctgcc
     3541 tccatagttg gaatcgttgc acaacaaatg actgtcatcc agtctgctcc tgcttttgca
     3601 acattactcg ccactgttcc tccagcgtct gcacatttcg tatcagcgac tagtttttta
     3661 tctgggaaca tactgcgaat acagcgaatc gctttttgtc cttcctgtaa gcaaagaatt
     3721 gtgcccactt caataatatc tactacatca ccaactttca taacatctcc taatgcatgt
     3781 tctaagtcat tgtgatctaa cgcaatttgc aaattcggcc tgctcatctt agttccttcc
     3841 tttacttgtt tttttggtac cactttgttt cagcaaattt ttcactgatt tctttgactg
     3901 acatcacatt tttgataccg ataactgtaa ctcctttttt ctctgcctga gtaaacatat
     3961 tcaaaaaatt gactggcgta aaaactacat cgtattggcc agctgaactt tttccctctg
     4021 aaatggcaca atgatggatt ttggtaattg gtacgtttag ttctttcatt gctttttcca
     4081 cacttctcat catcatcaag cttgttcctg atccatttgc acacgatact aaaattctca
     4141 tcttaatccc tccattatga gtgttgtcta atcagatttt cttttatact ctctgctttt
     4201 ctgcatatgt gtcatagtct tctacaatca aaaaataacc atcaggattt ttccgatatt
     4261 gtaactgcgg aatagccaat aaaccaatga caactacggc aattccagca taacctagga
     4321 ctttcatgat tactgtaaac aatggccaag ctgttgccca gtcgaacatc ccgatataac
     4381 caccgtatgt tgacatccca atccatgtag cgaataatgc tgaaccgcct acttgtatga
     4441 ttcctgaaag aaacgggaac aggcaagcgg ctttgaatcc acctcggtta ttggcataaa
     4501 ctgctataac ggcattatca aagaacaacg gaatgaaacc ggctatgacg atcgttggtg
     4561 atttcaataa aatcagtaaa ccaatcatca aaaactgtcc caaagcacca aacaagaacc
     4621 caatcgttac cgcatttggc gaaccaaagc caaaagtagc agctacgtca attcctggaa
     4681 cagctcctgg taaaatcgtg tttgagattc cttggaaaga ttgagttagt tcatcgacga
     4741 atgtccgaac acctaattgc aagatcgcca gatataccgc aaaatttaaa gaagtcgtca
     4801 taatgtagaa taagaaactc tgcccttctt gcatgaattg cgcttgaatc aaataatctt
     4861 tgcctagtac aatcagaatg atcccaaaga aaaatagcat gagtaacgaa gtagcaacca
     4921 tattctcatt gaaaatcgac aagaatcctg gtaatttgat atcttcaatt tttttgtctt
     4981 gcttgattga tctatttttc ttttcatccc gctttttcat ccactcagct aattttgcaa
     5041 atataaagat tccgaacatt tgctgatgtg ccactgcaaa tccggcacct tctgtcaatt
     5101 cctgcgtgat tcctactgtt aagttagaag ctactgtcca ataacaacct aaaatcagtc
     5161 ccataaaaat caatgcttgt atcctaccta gatcaggaaa gcaaaacagt aaaatccaaa
     5221 aagcggtagc tgcttgttga acctgaacgt ttccggtagt gaaaacggaa cgcagtttcg
     5281 tatatttctg aaaacggact aataagatat ttaaaacaaa tgcaatcagt aataaaatca
     5341 tcacatctcc aaaggtacgg ccaaatgtct ccattaatcc agcatccaca gcattttgtc
     5401 caaaataagg atctgtaacc atggcatcca aattgaaccg ttcctttagt ccaaccaaga
     5461 ttggccgaaa attattgacc aaaccaccag agccaacagt caaaataaag taaccgaccg
     5521 ttgcctttaa gaatccagcg atgcattcat gaagcgggcg tttcagcaaa atatagccta
     5581 ataacacgat aaaaccaatc aggtatgctg gctgcgtcaa gatatttgtt gcaaaatacg
     5641 tccaaatgct taacaaaaat tcccccatct tttttctcct ctatcaatta attatattct
     5701 gccatcacaa cttggtaatc atcgatggag ttcgtagaga tgagacgctc aaccaatcca
     5761 tctgtcatta ataattcgga taatttttgg atattttcta gatgttcttc tggatctttt
     5821 gctgcgagtg taaaaaacaa actagccgtt ttctcctcat tctccccttc actttcaaaa
     5881 cggattggct gtttcaactt ggtaaatgaa atagctgttc caaaaacacc atcacttttt
     5941 tctgaagaat gcggcattgc tacttgaggt gcgatcacaa tataaggacc gtatttttct
     6001 acacaatgga tgatctcctc cacatacgtt tcatcaataa tttccttatc gatcaataca
     6061 agacagcttt cggcgatagc ttcacgccaa tccgtgattt ctttatctgg gtaatgaatc
     6121 aattgatggt caaaaaagta tttcagcaag aaactcccct tctttcttat aaaaacgacg
     6181 gaaacggtgt atcattttca atcgagaaga catcttcaaa tcctcgataa aaattaaact
     6241 caagatcatc tttgtttgtc ggatagatga atttcccgcc tacttgccag atataaggtt
     6301 taaatccata cttcaggcga tcctttttga atttccaaat ttcagtgatc tcttttggat
     6361 cagccataaa attagaccaa atatcgtagt ggacaggaat cactactttt gcattcaatg
     6421 actccgccat gcgcagcata tcaacagaag taactttgtc tgttatccct cgtgggtttt
     6481 caccataggc ccctaaacag acatcgattt tgtgttcatt accatgtttg gcaaataaat
     6541 tggagtaatg tgaatctcct gcatgataaa gattccctcc tgatgtttcg aataaatagt
     6601 ttactgcaat ttgatccatc tcctgaggca tttttccttt taatacctct tctggatctt
     6661 cacaagtgac caacgctgtc cgatcaaacg cttctaaagc tactacattg atgtctttga
     6721 ttttgactgc atcacctggc tttactgtaa tcgttttttc ttcaggaatt ccccattttt
     6781 gccacgtgcg aaccacttct tttggcccaa tgaatgttgc ttcaggacaa ttttgatgaa
     6841 ctgctgcggc tgtatggata tctagatgat cggaatggat atgtgtaacg attaaagcgt
     6901 ctacattttt tatcgcaaaa ggatcgatta cgaaaggctg agtacgtaaa ttaggctgca
     6961 tattttgaac gccactcata cgcatcatct gatggccttt tttcatcatg ccattcccat
     7021 gactacgttt tcccgtccca caccatagat cacataaaat attcgttcct tcatgacttt
     7081 tcagccaaat tcccgtacag cccagccacc acatagcaaa agttccagga tccactactt
     7141 ctgtttcgat ttcttcattc aagtatgtgc cccactcagg aaatgtttct actaaccact
     7201 tttcttttgt tacttcattg acagttttac tcatttgctt gctcccccca tatcgttgtt
     7261 ccaacttaca agtaaatatt aatgtgtatc agcatccatg aaaacccttt tatatgaccg
     7321 tttgtttttt atatgatttt ctttcagtaa tcaatgattg tttcagtata ataaagacag
     7381 atatgaatgt ttaaatgaac aactagtcaa ataaatccat ggatcaagat gtccctcagg
     7441 aggcaaaagt catgaaaaac tcacttgtca atattgaaaa acgtcaaaaa gaaattttgg
     7501 aactgcttca gcagactgaa caactatcaa ctttcgaatt agctgaatcc cttgatgtat
     7561 ctattagtac gatacgaaga gatttaaatt tattagaaga aaaaaacgag atcatacgca
     7621 attacggtta ttgttcttat aattacaaaa accagactga ttttgatcaa tctggtcctg
     7681 aaaggattaa acaagcgatt gccagagaag ctagtaaata cattaacgat tatgatacgc
     7741 tgtttatcaa ttctagctcg actgcgttga aaacactgga ttttcttcaa tctaaccatt
     7801 tgacagtcat tacaaacaac gtgaaaatca attactctcc gcacaaagat aattgtagtt
     7861 atattttaac tggtggagag atgcgctttc caaaagaagc actagtgggc gacatcgcat
     7921 taaatacgat ttcttcagtc aatgcagaca tttgtatcat tggctgtagc ggagtcgatg
     7981 tagtacatgg ggtcacaaca aagatattaa atgaatcaac gatcaacgaa atgatggtca
     8041 acaagacaat caaatgtaag attttagtcg ctgatcatcg aaaaatcgga ctgacatcaa
     8101 agttcaagat tgctgatatt tcagtctttg attatttgat cactgataaa ttttgttctg
     8161 aaaaagtatt agaggagatc agaaatacgg ggataaaaac cattcaagta gattcatttt
     8221 aagaaccagg acgtaaatat gtaaatagga ttatcaaaaa aatgaataac aaatttatcg
     8281 ataaatgaaa aacacgctac gctcaaattg ataaaggaat cccaagatgt atccatccaa
     8341 tcgataccat cataatctat tgcttctttt ataaccgtta tatgttgcct aaataggaat
     8401 caaacaatat cgaaaaaagg aggtatgaca aaagtcacac ctcctttttt cgaataaacg
     8461 gtattcaaat cagaacactg atttcacaac cactttattt caggtcgttt tcaatcacct
     8521 ttaccgcgta atcatttccg ttgaaacgcg ctattttttt tgcgtccata tatctttcta
     8581 aataatcatc ttctcctaat tgcttatggc aagaagcaat ttcataaagc aagtattcca
     8641 atgcataagt cgatttatgt ttcttattat actcaatacc cttctcacat aattggattg
     8701 ctttatcata atcctcgatt ttacggtaat aggtggcgat atcataatat acaatgcaga
     8761 ccatcagatt atcaaaatca gtctcactca atattttaag tgtacgctcc acataaactt
     8821 tcgcaaaatc taattctcct tttgacaagt agatcattgc cacgtttgca gctgcttgta
     8881 agtgatggat atctgtttcg gtcgtttgtt ccaacacttg attaaaataa aataatgctg
     8941 catctggttt tttcgcataa agatgatcaa gcagtccttg ataaaataaa atttgattta
     9001 aaagatcaga attacttact ttttcctgat cgatcgtttg taaaatttta taagattctt
     9061 ggtattttga tacagaattc aatgattgaa tctcatttat ttttttcctg attttgtctt
     9121 cttctgtttc tactaatata tcggagatct cgacattcag ctttttgcaa agcgcactta
     9181 agatattgat gcctggcgac atattcttgt tttcgatatt gctgacagtt gcttgttcac
     9241 aaatgccttc tgctaattgt acttgtgtta accccttttt ctttcggtat tcttttagta
     9301 atgctccatc tactttaatc aaaacaattt ccttcccttt aaaaaattat ttctccttta
     9361 tatcatacac aaaaaaaagt tctaaacaac caaaaatgtt tagaacagaa taaaaatttt
     9421 aattttaaaa taatattcaa attaaacaac tggcgtatct gctccttgat ttctttttgt
     9481 gttatctgaa taattatcgt ttacagttat aacacatagt gaaaattgtc atggcgagaa
     9541 agtctaagaa tatttttaaa cataactatt tctttcttac gtgtgtataa tgacgacagc
     9601 acacctatca attaatataa ttattttatt tttttatgtt ctcatgttta tttttaatga
     9661 cacaacttct cgtatttatc tggcacaaca tataaatcga tcaaaaaatt gttgcgtgtt
     9721 tttttgaaaa ttggagtttc aacttgatta caaaatttaa atttagaagt tgtgaaaaca
     9781 gcgagagaca gtttaatatt caaaatatac ataggctact cgaacgctgt gttttttagt
     9841 atactagtaa gaaacgaagg gatagactca ttccaataag gaggatttca tgaagacaat
     9901 tctttttgct cctgcagtat tcaatttagc tgagacaaca cgaatgattg aggttgccaa
     9961 agctatgaaa gaccgttatt actgtgtatt ctttggcttt tctaaaactt ttgctccttt
    10021 gatcacagaa agtggctttg agtatcattt actagcgccg atccttacga aaaaacaaga
    10081 agaacaaatc ataaaatttg accagttgcg tacacttaaa aaccctttta ccaaagaaat
    10141 ggttgcctct cgtatcacta gtgaacagag tctgattgct aagtttgagc ctcgactgat
    10201 tgtgacaggt tcgaatgtga caattttttt atcggcccgc aataaaaaga ttcctttagt
    10261 atatgttaaa ccctatgctt tgagtgaacc tttttttcaa tcagagcatg cgctgatgcc
    10321 ccacgagtta cgtttcttcg gccctttaag caatcccctt tggcactgga caaaaaagcg
    10381 tatagtaaat acccgatgga tccccaatgc cttcaaacac gctgcacaaa aagaaatgct
    10441 tcctttacca aaaacaagtt tacaaatgat ggatgctgat ctaaatctca tcacaacgcc
    10501 taaactattt ttggaagaag aacagctgcc ggaaaattat cgaatcgttg gtccaatttt
    10561 tgctaagctc agtacttcct tgcctgaaga agtgctaact tttatacagc gaaagcaaca
    10621 agaaagaaaa cgtattatct atttcgcaat gggtagttca ggcaatccgc gtatgttacg
    10681 caaaattttg ttcttcttac aaaaacaacc ggatctagcg gttattgcac ctattgacta
    10741 tctgcttgat gacattcaat ctgactcaga agatctttta ataacagaat atctgtctag
    10801 tcatcttttg catgaattca ttgatttttc ttttatccac ggcggggaag ggaccgtcca
    10861 aactgcctgt gaatccggca aaccgtttat cggtgtgggg atgcaatttg agcaaagctg
    10921 taatattgac tattgtgtaa aatacggaaa cgcaatcgcc ctcacaaaac cagtgactga
    10981 aagaaaactg cagattgctc tgcgcgaagt ttcttcaccc aagattcgtc agcaggcacg
    11041 cacgctgcaa gccgtttttc caataaatgg tccacaacaa gcaattcaag aaatcgaaac
    11101 cttcctgagc aaccacccaa tatagtttca agtagaaaaa gcttttttaa tagagactca
    11161 tcagtaattt cgctattcga gacccagaca gtgaaaatac aaaaaaagta aatagtggat
    11221 tgaaaagtta gagttactta aattacggtg tctaaaaaac acaaaatcat ccttaaaata
    11281 aaagatctga cgctatccaa atgagcatag gtagcgtctg ccatttattc ttagtcaaat
    11341 tctttgacac ctttctctgg ttggatcatc tcaacgtttc ttcaaaagaa agaccataat
    11401 aattaggcaa atacattttc ttaaaatgtg tcatatagat ctagaagcat cgctttgtag
    11461 agatttgtgc cgctagtccc ctcaacaatt actaaatcaa atgtaggatt taccacttta
    11521 tctttgataa tcaacctccc tcttctgttg agacaatgat ctcgcaatcc gatgcatact
    11581 cctatataaa ctgccagcaa tccccacata ttgagtagat catgtcagaa tgttctatgt
    11641 cgtcagcaac gatacactaa aagtcacgtt caccatgact gattgctatt cccagcacaa
    11701 ttatttctat acacgtttca tgatttgaat aaacactcac atcatacata ttaccattca
    11761 tacaccatac tgcagcccct actatatggt tgtaagctcc caaatctata tttgacttga
    11821 tcactttggt agtagatcta ctaattctaa atccattgct gataaaattt ccattttttc
    11881 ctccttcttt ctgccaaaac agttaaaaca ataataatct ttatgtaaaa aagggctgtg
    11941 acaaaagcct tgtcacagtc ccatattccg aataaacggt ggtcaaaagc ttgcatctgc
    12001 ggtaataagt tcaaccaaac gaaaaataag ccaacatttc acaaaaattt aagaagcaat
    12061 tttcgtaaat ttcttcttat ttcttgaagc taaccacttc tgttacaacc acttttagaa
    12121 catttaaagc ttcgatgtga caaaccatct atgctgctcg atcataaaat tgatagcaaa
    12181 aattccacaa agtacatatg tcccccatga aaacaaaaca ttcatatgcg gtacaatgat
    12241 ctttggaaga atcacgatca aaaatgatgc cagtgcccaa atccatcgat tgatttgaat
    12301 atgatatgta gctagcatat tcaatataag gtgactagcc gttaataaaa agataaaaac
    12361 aaaaaataaa atcagccaat acattgtttc ccaccttcta aaaaccaaac ttagacagtc
    12421 tggacttcgt tttctccacg actttgttca tctgacagca tctttttatc ataagctttt
    12481 acaaatggat aatagataat aaatgctatc aatgtgttga tcatgatcaa gataacaaca
    12541 cgccagtcat ttcctgctga aagataaccg ccgattggag ctggtaatgt ccatgttggc
    12601 aaaacagtaa atctattgac catttctaat ttgactgcaa aataactgat aatagtcaaa
    12661 actaaaggaa ctaagttgaa cgggatgaac atgtaaggat tcaacatcat aggagctcca
    12721 aaaattatgg gctcattgat attgaagata ccaggaataa tagaaaaacg accaacctct
    12781 ttcaagaatt ttgatttaca gaagaatata aacaaaacag ataccacgat cgttgctcct
    12841 gcaccaccaa ttgttaccgt ccattgataa aactgttcag gagcgatata gggtaattgg
    12901 gtggcagctg taccatctgc caatgccttt gcattctcat ccaacatgat cagccacatt
    12961 ggacgaacaa cagcccctac aatactcatg ccatggatac caaatgacca caacaaatgg
    13021 atcaaaaaca ttggtagtaa aacaccaagc aaattattcc ctaaaatatt ggttaaaggc
    13081 tcgaataacg ataataaggc agcgtttaca tcaaagcccg cgacgcttcg aatcagccaa
    13141 gtaattgtta aaatgactgc acctggaatc aatgcctcaa aactacgggc aacagcaggt
    13201 ggcacttgtt ctggcatttt gatagtaata tttcgatttt taaagaaacg atatacttcc
    13261 actgcaaaaa tcatagataa gatccccgca aacatcccag atgctcccaa agaactcata
    13321 ggtaagaccc agccaagagg tgcctcttca ggagttaaaa cgtttacagg aacttgtaac
    13381 ataaagaacg ttcctaaaga taatatacca cctgtcacac tatctaatcc atatgattta
    13441 gctagatccg aacccataac aaaagttgca taaatagcca tcaatcccat cgtcatccta
    13501 aagggtaaaa caatcgttgc cgaataaggg gcaataaatt cattccatcc aggaaacggt
    13561 aaattgataa agaccataaa aaatgtccca atcaaaatca atgacaatgt tgaaacaacc
    13621 ccattacgaa ttgctaataa atgacgctgt gtaccaattt ttgccattgg tgggacaagt
    13681 ttctcttcga cccatttcat cagcttttcc actaaaacca ctccttttca ataaaatatg
    13741 aaccattcat tgcatgacaa cgcaaagaat gttcataact aattatataa ttaagaaatt
    13801 ttatttcaat atattctctt aaataatcaa atttattttt aagaatcctg aaaataagtt
    13861 ttctaaatca taggagcaac aaaaaaattc gataattaac aaaaatcaaa atgattaaat
    13921 ctagtgattg aaacatatca tttcaaaaaa attttgtaaa ttcacccata aatatcaccc
    13981 tatttaactt ttttcatcgt acactaaaat tcaaaacaac tatctttaat ctttacttct
    14041 tggttttgtt ttaataaatt tccttcaaaa atgcctggag aaactacaat gatcaccgta
    14101 tctttttgtg cttgctccaa cttcttcaaa tctataatag ctaacagtgt cccttctttt
    14161 acctcctggt tactttcaac ccgtatctga aacggacttc cttcgagttc aactgtatct
    14221 aaacccatat ggatcagtat ttttctgcca gattcacttt cgatcgttat ggcatgtcgg
    14281 gtaggaaata tattatcgat tattccttta actggcgcat ggatatatcc atcatgattc
    14341 gttatagcgt atccaactcc tatcattttt gaagaaaaga cctcgtcttt tactgctgtt
    14401 aatggaataa tttgaccact agctacagct tttagaatgt ttcttgtttt aaagaaatgc
    14461 actttttatc acctctactt taatgctggc caatattttt tgtttacttt gatcatttca
    14521 tctagtattt tttttgcgac tctggcattt ggtacggttt gattcaatgt aaatgcttgt
    14581 aaagcttttt gataagaacc ttcaaagtaa gcatctacta gtaacttctc tactgcaact
    14641 tgtgcctcca tcaatccttt atgaaaatca ctgattggtt ttcttagtgt aattggctcc
    14701 acaccagttg caccaacata tgccggtatt tcaacaactg catctgaccg tagattcgga
    14761 atcgcccctt gattaggaac gattagcata aagcgacgac gatcatcatt caacaaggaa
    14821 atagctatat cgacgatata ttgtccatgc tccccaaaat taaagttcaa tacttcttcc
    14881 acaataccgc ggcgtatttt atcagccatt tctttggtat ttttctctcg tccatccatg
    14941 accatattag cacgtgtata ctctttgtca gtatgctcaa cgaccatatc aggatataag
    15001 taatactcta agtaattatt cgggatatta cttggaaaat ttttcgtgat gactgacatc
    15061 atttgaaagg ttttttgcca agttttatct cctatattaa aatcggcgac ttgcatttct
    15121 tttgttatta gtttttcaat aatctctggc ataatttctc gttgcaattc tttatcataa
    15181 atcgatgtat accagccaaa gtggtttaaa ccataatacg taacgatcca attcttacga
    15241 tcataaccgt aattcactgc aatcgtttct tcaattgaaa tagtcatatc acaggcatta
    15301 atcattttta cattcggata tttacgacga acagcttcag aaacgatcga ctctggattt
    15361 gtataattca aaatccatgc tttgggggca tagtcttgta tatgatcgac aatttccaat
    15421 aaacctttga ttgagcgcat cccataagca aatcctccga gtccacacgt ttcttgtcct
    15481 actaatccat gtttaagtgg aatcttttcg tccttttctc gcatctccaa tcctccaaca
    15541 cgaatttgtg agaaaacaaa atcgcatcct gtaaaagcaa gctttggatc atccgttgca
    15601 cgaatgcgaa tatttgaaaa accttttctt ttcagcataa atttaacaat caagtacata
    15661 tccatatttc tttcttcatc aatatcatat aaacgtattt cagataatgg caaccgtttt
    15721 tcattgttca aaatggcttg aacgattcct ggtgtatacg tactcccgcc tccagctata
    15781 gtgataatct gttttttcat catcatcttt tcctttctat tcttctaata atttattgaa
    15841 ttctgccata acataagtca cttctaagcc gatgataatt tgataatttt tttcagttgg
    15901 tttaatgatc ccgcttatcc ctaattgctt gattcgctgt tcctcgacca tggtgggatc
    15961 cactacctct acccgtaggc gtgtattaca actatagaca tccaggatat tggcttttcc
    16021 tccaaacgct tgtagcattt ttgttgccat atattcataa tttttacttg ctaaccctaa
    16081 ctctgtttct tctaaggtta cttcattgcc aaactctgcc ttagcttcac gtcctaatgt
    16141 ctgaatgttt ctcttattga tcaaataata aaaagtaacg taatacaagg caaaaaagaa
    16201 aattccgatt ggaagaatca aaatagcatt ggtagcgatt cgaaaattga tgagataatc
    16261 aacaatacat gctccgaatg taaaaccaat tcgtatattg aataagtaac agacaatccc
    16321 tgctaaaccg gcaaaaagtg catggatcac gtacaacata ggagaaagaa acatgaaact
    16381 aaattcaaca ggctcagtga tatttgcaat aaatgaagtt cctgcaccgc ttcccatgat
    16441 tcctttgacc atctctttat tttctttata agctgcttta tagatagcta gacatatagc
    16501 tggtatacca aacatcatta ctacaaaaaa aactgataaa aacagtccag cctgaggatc
    16561 tccgttgata aagcgtgtca tctctcctct gaaaacttcc ttgctaggag atgtatagct
    16621 tcctaaatcg taataaatat acgtattcaa cacatggtgc agtccaaagg ggatcagcaa
    16681 acgattcaaa aacataaata tccccacacc taatgctccc atatcaacga gcagttttgc
    16741 aaaattatca atcccaattt ggatgagcgg ccagataaag cccaaaaaaa cagctgcgat
    16801 tgtttgtaga atcatcacca tagtcaatgg gaatttttcg cccgcaaaat aagaaaaaac
    16861 tactggtaat ttttgttctt taaagcgatt atacgtccat gccgctacta aaccggaaat
    16921 aattccacca aaaatcccca tattcacttc tgggtccata atttctaagc cttttttcaa
    16981 ggtagcaatc gctaaaaaac cagtaagtgc aggtattcct ttgtccttat ttttgacaaa
    17041 accgattgca actcctatag caaataagat atccaagttt ccaaaaacgg catttccagc
    17101 tgcaccaatt actgggatgt tcagcatatc gtcagctgat aaacgcatga tcaatcccgc
    17161 taatggcatc gctgcaactg gaagtagcat cgctcttcca agcttctgca catttgattt
    17221 aacattcatt tttattcctc ctctattaaa tatcctcata tgttactaac tcaatcaaag
    17281 gatcattttt cactttttga gcaaatgctg aatgaattaa aaattcgact tggtgggtac
    17341 gaggatacac aaaacttgaa ccatttaaca attcctgatc caaatacgct gcgtgacaca
    17401 tgacttcgat cgttttatct tctctcaata tcatgagtaa acttgccaaa tattcttcca
    17461 cactcgtatt gatttgtcgt tttttttcat cagtttctcc tacgacatcg aaacaaggct
    17521 caaaataatc gacatgacga acttcatctt ttcgttcaaa gttgctccga accggcaaat
    17581 catatttttt tgctaatgta acaactacct cctgattttc atccaaagta tgagtatgat
    17641 gatgactgtc taaatgagtt ggtatgatcc ccgcatgata aattttttga atctgagcat
    17701 tccattcctg atacagttga tctttgtcaa tgttaaatgg atgctgataa taagataagg
    17761 atctgaactc tccgctttcg ataagagtat cgacagtttt gaggagtggt ttgccacacg
    17821 tcaatgttaa atggactcct acacctagcc taggcatttc ctttcttagt ttcacagcat
    17881 gctcgaaacc cggcatattt gccatcaatg tagttgatgt caaaattcct cgctgataag
    17941 catccataat cccataattc acaccatggc tatagccaaa atcatccgaa ttaaaaatca
    18001 cttttcccat ttcattctcc ttcttttttc tactaaaata ggcgcaacca aataatcata
    18061 gtccatttac tatgtgtcat ttggttgcgc ctatagatag ttacgccctt attttaatag
    18121 attcttgcta gatgaattgc caaatagatg atttcatttt cttgaatggc aagttgaaat
    18181 tgctgttcga tgatatcttt aatctttagt gctgtacaat acgctttgct atgtttaaat
    18241 gtttcagtaa aaaaaccaat attttctact ggcgtttctt cctgataaat caagcgttta
    18301 accaggaatt tgatatgaac cattaagcga tgataagtca gtgattcttt atcataaacg
    18361 tttgaatagg atttttctag atgggacaaa atcgtggtcg tcatttctac tatttttaag
    18421 ctctttaaac tattaggctc tcctagctca gcattaatga tatgcatagt gatgaatcct
    18481 acttcatttg accctaactc ggtttgatat cgatgattga ttcgctcgac cacttctact
    18541 gctatatcat attcatttgg ataaaggaat ctcatttctg gcaacataat tgctgtgaca
    18601 tctctatctt ctctttttcg ttgaatagca aaataaatat gatctgtcaa tgtaatatag
    18661 atatttgaat ctaatggaga ggagatccta gctgttatca gttcaatcac ttcttccgca
    18721 aaatccataa attcgattgg aatctgcgag actatctcac tgaatcgttt gatcttttct
    18781 ttagaatcta atacaaataa tttgtcagca tcctcaacat caaatggctt actcacttta
    18841 gcagaaaagc caatcccttt ccccatcgca attttttcta ttccatcgtc atcgatcaat
    18901 acagcattat gattcagctt tttcatcaca tacaccgtta attcacctcc tctatatgat
    18961 aaaacaaaac aacttgcaga ctccttcata catatttttg tctccaaaca atttttaaag
    19021 acaaaaaacg acctaagaaa gaaccctttc ttaggtcgtg cctaattgaa tagtagcacc
    19081 ctacataatt tacaaaaata gtttaacaca taataaaagc gttatcaata tcttttttac
    19141 tcgttttatg ataatacttg tatcgaattg atttggaggt aattacataa aaacaaaaaa
    19201 aatatagcaa ctctctataa gaaagttgct atattgataa ttataccggc ggccggggtc
    19261 gaaccggcac gtcctcaacg gacactggat tttgagtcca gcgcgtctgc caattccgcc
    19321 acgccggcaa atttaaaaat aataactggg gtagctggat tcgaaccaac gcatgaggga
    19381 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaagggcg aatgatggga
    19441 atcgaaccca cgaatgccgg agccacaatc cggtgcgtta accacttcgc cacattcgcc
    19501 atcataaaag atgttattta atttctcaat aaaaaactaa gatgaagaca tcttagtcaa
    19561 taccggcggc cggggtcgaa ccggcacgcc ctcaacgggc actggatttt gagtccagcg
    19621 cgtctgccaa ttccgccacg ccggcaagtt gatttattag tactaaggcg gtaaccggat
    19681 ttgaaccggt gatgaaggtt ttgcagacct ctgccttacc acttggctat accgccatta
    19741 tactattatc aatctataaa ataaagaata aattatag
//
LOCUS       DARBEC010000055        19130 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.056, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000055 DARBEC010000000
VERSION     DARBEC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19130
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.056"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..703)
                     /locus_tag="SCL80_001803"
     CDS             complement(<1..703)
                     /locus_tag="SCL80_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107665.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFT"
     gene            complement(717..1641)
                     /locus_tag="SCL80_001804"
                     /pseudo
     CDS             complement(717..1641)
                     /locus_tag="SCL80_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            1758..2837
                     /locus_tag="SCL80_001805"
     CDS             1758..2837
                     /locus_tag="SCL80_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1107666.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            2869..3462
                     /locus_tag="SCL80_001806"
     CDS             2869..3462
                     /locus_tag="SCL80_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107667.1"
                     /translation="MTKSLQTILESTKEIRVLGIGHCAKDALVLYETHRPDVLLTDIR
                     MPKQTGIDAAKEILAKFPKAILLLLTTFKDEEYIREAFSIGVKGYLIKQNLQAIIPSV
                     KSAYNGQAVFGNEIIETFTQMMKNKPTIDSHAYASFSERELAIIKEVAAGKNNKEIAD
                     ALYLSDGTVRNYISQLLEKLDLRDRTQLAIYFYQHLQ"
     gene            complement(3572..3799)
                     /locus_tag="SCL80_001807"
     CDS             complement(3572..3799)
                     /locus_tag="SCL80_001807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1107668.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(3902..6490)
                     /locus_tag="SCL80_001808"
     CDS             complement(3902..6490)
                     /locus_tag="SCL80_001808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEG1107669.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(6510..7169)
                     /locus_tag="SCL80_001809"
     CDS             complement(6510..7169)
                     /locus_tag="SCL80_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1107670.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVAQHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCL80_001810"
     CDS             complement(7218..7721)
                     /gene="trmL"
                     /locus_tag="SCL80_001810"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEG1107671.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(7942..8808)
                     /locus_tag="SCL80_001811"
     CDS             complement(7942..8808)
                     /locus_tag="SCL80_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1107672.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            8917..9558
                     /locus_tag="SCL80_001812"
     CDS             8917..9558
                     /locus_tag="SCL80_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEG1107673.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCL80_001813"
     CDS             complement(9620..10981)
                     /gene="mgtE"
                     /locus_tag="SCL80_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEG1107674.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(10999..11898)
                     /locus_tag="SCL80_001814"
     CDS             complement(10999..11898)
                     /locus_tag="SCL80_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEG1107675.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(11900..12697)
                     /locus_tag="SCL80_001815"
     CDS             complement(11900..12697)
                     /locus_tag="SCL80_001815"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEG1107676.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(12675..13379)
                     /locus_tag="SCL80_001816"
     CDS             complement(12675..13379)
                     /locus_tag="SCL80_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEG1107677.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            13510..14085
                     /locus_tag="SCL80_001817"
     CDS             13510..14085
                     /locus_tag="SCL80_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEG1107678.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            14203..14880
                     /locus_tag="SCL80_001818"
     CDS             14203..14880
                     /locus_tag="SCL80_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEG1107679.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
     gene            complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCL80_001819"
     CDS             complement(14959..16767)
                     /gene="pepF"
                     /locus_tag="SCL80_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEG1107680.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
     gene            complement(16943..17941)
                     /locus_tag="SCL80_001820"
     CDS             complement(16943..17941)
                     /locus_tag="SCL80_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEG1107681.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            complement(18167..18820)
                     /locus_tag="SCL80_001821"
     CDS             complement(18167..18820)
                     /locus_tag="SCL80_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEG1107682.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
BASE COUNT         5525 a         4137 c         3324 g         6144 t
ORIGIN      
        1 acgtaaagct tgctgccaaa ttagcgattg aaactaacga ttgctttcta ggaatcaagg
       61 acaagcgaat accatttgac gattgatttg cttgctgatc ctgtacattg cggacccccc
      121 acatcgtccc gtagagaatc gccattccaa tcagagtgaa gaaataaaaa cttttgaaac
      181 tcatattacg acctgcatga acttcttgaa aagatacttc ttgttcaaaa acagtcatga
      241 tgggcgcgag atcaacgttc tccacttctg ccactttact cgaaattata ccaatacgct
      301 gtaaaaattg atccatgacg tttttcatca acgtctgact cattcctgct tgacccacat
      361 ggagcgaaac agatgaacca tcaatgagat aaaaaccagc caaatcatct gcatttagct
      421 tttcattcgc ttctacttcc gatagttcct gtacttcaaa taatggttta tcattgactt
      481 cgacttgttc taagactgtg gtaaaatttt ccgaaagcgc tgtttgatcc gctttgatta
      541 caattccagt attaatcgtt tcgagattgc caccttgatc aatctcgcca aaggccgcca
      601 taaacatcaa tcctaaaaga attggaaagg ccaatgtcca aaacaacatg gatttatttc
      661 gcagcagtat ttttgtgcga tatttataaa aatgccaaaa cattatttcc cctcctctag
      721 tctctcaaat ccttgccagt aatctctaaa aagacatcat tcaaggacgc ttctttggtt
      781 gctatttttc cgtaactgat cttttcttct tgtaagattg taagaatcgg caacaatact
      841 gtatttagtt cagtagttgt tacttggagc tgaccttttt tataagcgat tccatcagaa
      901 gctattgttc gctgacaaca cgttttctgg gagtaatggg atgtcgatcg tcaaacgatc
      961 cgccgtccgt accatatctt ttagagattc tttggttcct ttagcaatca cctgtccttg
     1021 atccatgatc acgatctggt cgcaaagaat ttctacttct tccatgtaat gtgttgtata
     1081 gacaatggtt gctccttgtc gattcagctc cttgatactt tctaaaattt tattccgact
     1141 ttgggggtct acagctactg tcggctcatc taaaaaaatt aaccgcggct tgtgggcaat
     1201 cccacatgcg atgttcaacc gacgtttcaa gccaccacta agttctcctg gacgaaacga
     1261 aacatatttt tctaatccca ctaattgaat cacctcttca atcaaggctt tccttttctc
     1321 tttttcggaa acatataaac cgcaaaaata atcgatattt tcttgcacac ttagttcgtc
     1381 aaagaccgca acgtcttgag gaacgacccc aatcattgcc ttgacagcat aattttcagg
     1441 agtcattgtt gtcccgaaca atttaatggt tcctttgtca taatttaaca aagaaagcat
     1501 acagttgatg gcagtcgatt tcccactgcc gtttggtcct aacagaccta agacttctcc
     1561 ttcttgtact tgcagatcaa aatgattcaa tgctacaaaa tctccatagc gtttgactaa
     1621 tttatcaatt tcgataatca tcatgaaaaa ctccttccaa atttcttgtt gatctgacta
     1681 taataaagtg caagaaaaga aactagtgac gattggctaa accacacatg acatttgtca
     1741 caatgaagga gtctgctatg cataaatctc ggctactcaa cgaaactagc tttctactgg
     1801 ttatctcatt gcttttgctg gtaagtcaag gattcgttgc tatgcaagtt atctatttat
     1861 tgatcgccgt tttattcact ggtgtcagtt atttactgcc tacaaagcca aattggattt
     1921 tccaagttat tctatttctt ttatgtgcct ttttacctga tcttgtcctg tttctgccag
     1981 cgacgatgcg agtgatcgcc cctgaagaaa gccaccttga ttacttgttt ttgttaggtt
     2041 ttttgttgat ttcgacggtt ggtaatcatc cccccatcaa ggtcgccttt gtcgtaattt
     2101 tgctgttttt ttccttttac ttacgaaaaa aagatcaacg aacgaccgat cttgagaaaa
     2161 agtacattgt ctccaaagat actcattggg aaaaacaaca agagttatcg gaacaaaata
     2221 aagcattggc cctgtcacaa gaaacacaat tagccttgca aattgtggaa gaacggaacc
     2281 gaattgcccg agacatccat gacaatgtcg gtcatctgct gtcaagtgcg attttacaaa
     2341 ttggtgcgct tgaaatgatc aatcaagaac caaaactaaa agaaccatta gaaaatttaa
     2401 gtgccaccgt tcacactggg atggatcgga ttcgtcaaag tgtccatgac ctccatgaga
     2461 catcgatctc ttttcaacac tccttagagt ttctgatcgc tgattttcct tcttctgtta
     2521 cgattgaagg aaacttgttt gaaggattga atgaaacgca gcaaaactgc ttcttaatgg
     2581 tcatcaaaga agccttaacc aacagtatca aacatagtca tgccaaaacc gttgttttac
     2641 attttcgaac gttgccggcc ttttatcgct tgcaaatcac gaatgatggc accagcccac
     2701 aaaaaaattc tgctcccggt attggtttga aaacgatgcg tcaacgtgtt caagaacaaa
     2761 agggacaagt ccatattttc caagaaaacc agcaatttca gttaacgatc attttaccaa
     2821 gggagggaca ccaatgataa cagtagtcat tattgagatg atccatttgt gacgaaatcg
     2881 ttacaaacca ttttagaaag cacgaaagag atccgtgtgt taggcattgg tcattgtgcc
     2941 aaggatgcgc tagtcttata cgaaacccat cgtcccgatg ttttgctgac ggacatccgc
     3001 atgcctaaac aaacgggcat cgatgctgcc aaagaaattt tggctaaatt tcccaaagcc
     3061 atccttttgc ttttgacgac attcaaagat gaagaataca tccgcgaagc attttccatc
     3121 ggtgtcaaag ggtatttaat caaacagaac ttgcaagcga ttattccttc tgtcaaatcc
     3181 gcttataatg gacaggccgt atttggtaat gaaatcatcg agactttcac tcaaatgatg
     3241 aagaataaac cgacgattga tagtcatgct tatgcttctt ttagtgaacg tgagcttgca
     3301 atcatcaaag aagtagctgc cgggaaaaac aataaagaaa ttgcagatgc cttgtacttg
     3361 agtgacggca ctgttcgcaa ttacatcagc caattactag aaaagctaga tttacgggat
     3421 cgcacgcaat tggctatcta tttttatcag catttgcaat aagtcttatc taacacaaaa
     3481 aaacctgaat gatgctcctt cgcatcattc aggttttttt cacatcttct attcaattgt
     3541 cccgcaaacc atttcctcac gattgaaaga atcaaggatt cgcagggctg ccaggattcg
     3601 ttggattgtt cggcgtgtta ggcggaaaaa taggcgcctt gtctaacgtt ttttctagtt
     3661 ttgattcttc acgatcgtct cgttctacat caggactttc attcaatttg aattcttttt
     3721 ccatctcatc taacttttta aagtcattgc cagattcttt tgctaatttc gctaatttat
     3781 cttcggtttc tttagccata aagtaacgcc ccccttttga tttcagctta gcatatcaca
     3841 ctgaaaaaac aaaagaaaac gctaaccaac gatcattttt agctatttct catctttaaa
     3901 atcaagcatt ttttgccggt ataaaatcgt agggagaact gttttccata ccaatcatcg
     3961 gatctactaa ctggttatcg atcacttctg ctgtaagaac gaaatcttta gaaacagcag
     4021 gttctacctc ttctttcttt tcttctccaa caaatgaaga aaacagatct ggttccactg
     4081 tttccattac ttggtttttc gtttcattcg tcccactgtc atcttctaca ggtagctttt
     4141 cttttacaac ccgtggctct tttgtatagt tcccatcttc aaatggatca tttccgacca
     4201 aagaatcttc ataagctgct agtttggtcc ggagagtgat attcatttta tcattatcca
     4261 tgatccttga tttcaacgta gtcatccggc ttgctgattc attcgctaca caagtgacat
     4321 aggcttgctc ttcccctaac aaaatcaaca gttccttact gcgagtcact gccgtgtaga
     4381 gcaaattacg ctgaagcatc cgctgatatt gatggaccat gggcaaaata accatcttga
     4441 attcactgcc ttgtgattta tggattgagc aacaataaga aagagtgatt ttattccatt
     4501 cgtttctttt ataagtgacc tcatttgcat caaactggat aactaattca tccactttat
     4561 cttcagaatc ttttgccaag atgatacccg tgatttctcc catatctcca ttgaatacat
     4621 tcaattcggg agtattgact aattggagga ctttgtctcc tatccgataa acggtgtcgt
     4681 tccaatgtac ttcctttttt ccaggtttcc ctggattgaa tatctcctgc atcattttat
     4741 ttaacgcatc gatccctgca ggtccccgat acattggtgc taatacttgg atatcttgtg
     4801 cacggaatcc cttatttttc gctttttcca caattttaga gatcaagggt tcaatttgat
     4861 aagcatcact tcggaaatag gaacgatctt tttgattctt tgtgaaatct tttggcagac
     4921 gtccttcttt gatctcatgg gcaagaggaa taatactaga accatcacct tgccgataaa
     4981 tctgattgag ttccattttg ggaatttgtt cgatttccaa aagatcatgg agtacttgac
     5041 caggaccgac agagggaagc tgatctttgt ctcctactaa gataacttgc atattcgtcg
     5101 gaatcgcttt gaataatgtg ttagccagcc atgtatcgac catagacatc tcatcaacaa
     5161 tcaaaagtcc gccttctaat tctttcgtac tgactgaagg tgttttttcc cgtccattca
     5221 gccccagaag acgatggatc gtactggcag gcagacctgt cgtttcgttc attcgtttag
     5281 ctgctcggcc agttggtgct gcaagtagaa taggaaaaat ttcttgggta tattttgaag
     5341 gatccaattc cagtccgttc aactctgcaa aaagattgac gattccgttg atcacagtag
     5401 ttttccccgt acctggtcct cctgttaaaa taaatagagg ggaacggatt gcttcagcga
     5461 tagcttgttc ttgcgaaggt ccatactgta tgccgaatat tttttcgata cgccgaatat
     5521 tcttctgcac ttctttttca ggataatgga tttctttttt acgactgaga aggcgctgga
     5581 tcgaattacc gatcccccat tcagaaaagt acaaactgtt ttcgtaaaga ttagtctctt
     5641 cttgttgtat ctttccttct tcaacaagtt ggataattac agtagcaacc tgatcaggct
     5701 ggatttcaat tgggcgactt gtttctaaaa tacggatcgt ttgctgaagc aatgtctccg
     5761 ccggtacgta tgtatctcct gtttgaactg catgctgaaa aatctgatgc aagatagctg
     5821 ctcgaattct tctcggtgaa tctgctgcaa taccgatttg ttcggctaga ttatctgcac
     5881 gtttgaaccc gattccttct atgtcttcga ctagctggta cggattttct tcgatgatat
     5941 ccaaagactc gttcttatag gcctggtaga tactgaaagc taactgacta ccgaacccgt
     6001 aacgattcag tcccatgatc acttgatcca tcccgtggtt tagccgaatc gtctcagcaa
     6061 gcatttctct ttttggcttc gtcaaaccgg agatttgttt taataccgaa ggatcagcaa
     6121 tgattttttc gatagcatct tctcccagca attctacaat tttttctgct gttttcttcc
     6181 cgattcctgg gaatttttcg ctggaaagat acgctacgat cccatttgca gttgttggct
     6241 tttcttgctc atatctttcg agtttaagct gccttccgta tttaggatga tccactaatt
     6301 ctccataaaa acgataagtc tcattttctt gaatatcgcc gaaacttccg gtgactacaa
     6361 tctctttttc acggtaatca gctgtggttt cactgatgtg taccaaaagc actttgtaaa
     6421 aattactagg attttgaaaa aaaatcgcgg caactgtacc aacgaaataa ggcatttctt
     6481 ctggctgcat cggaagccgc ctccttcttc tatagtaaag gttctggtgc tgtttcagtt
     6541 cctaaaaaat ctgtttggtt ccatactttc agttcatatg gcaataatct gcaaggctct
     6601 tcagtctcta aaatcgtcaa actactattg aacaatccac ccatttcacg caactcactt
     6661 agctcttttc ctgtcatcca ttgaatggct gcagttaaag aagctccatg tcctacgaat
     6721 aaaagtgggc catctttatg ttgggcaacc gcatctgcta caacagtttc gatacgtgtg
     6781 attgccgatt caatcgtttc tccgtgaaaa ggagaaggat catacagatc taaatgatta
     6841 cgcaaattcg gcagattatc cggatatttc tggtacattt catcgatcga ctgaccttct
     6901 aaagtaccca gccctagttc tcttagctta tctgtataaa aaatctctac cggacgagca
     6961 agctctttgt tgataccttc tgctgtatca cgtgctcgct ttgctgtact ggaataaatt
     7021 gctttaaaag gaacatattt tacctttttc ccaaatgctt tgatttcttc gtaactttgt
     7081 ggcaaaagcg gagaatcgcc attgctccct tgaaaacgtc tttcctgatt ccattgtgtc
     7141 ttcccatgtc gggtaaaata tagttccata cgcagcaaac tcctgtcttt cttttatttt
     7201 actctataat aaactgtcta atccagcttg tcattttcat agtgatgcgt caattctaaa
     7261 ttagggaatc cttgctggcg aagtgcttca tacactatca ttgcagctgt attcgataaa
     7321 ttcaatgagc ggacatgctc atcattcata gggatgcgta aacacttttc ttcgttttca
     7381 cgcataaatg tttctggcaa accggttgtt tcttttccaa ataaaaagaa gtgctcttct
     7441 ccatcagcaa aattttcgtc actatatact ttatgggcaa attttgagat caaatgtagt
     7501 ttacgattac ctaaaaatgt taaaaaagct tctaaattac tgtggtaagt aatatttaca
     7561 tcattccaat aatccaaccc tgcccgtttt agatgtttgt catcagttga aaaacctagt
     7621 ggctcgatta aatgaagcgg agtattcgtg gctgcacacg tacgggcaat atttccagtg
     7681 tttgcaggga ttaatggttc aaataaaacg atatgattca tcaggatcat tcctctcata
     7741 tttatgttcg tttctatttt ctgacaattt gatttttgca taaaaaaaaa cgtatcaact
     7801 cggtgtcagt cactgcgagt cgttacgtta gtgaagctct tctcactaac ttttatcaga
     7861 acaggaacct gataaaaacc aatgaaaaaa caacttgttg ctaatataac accctcaact
     7921 tttttttgca acctctactc attaaaaact gctagaaaaa cgttttctct tagttccttt
     7981 taataattct atgtcaattg taatcttctt caaaggattc ttatataagt ctgctttgat
     8041 tcgctcttct acctgccatt ctaaaggtga catggccaaa agatcttttt gattttcctc
     8101 tggtatctca aattcatagg ttacacgctg acgttcagtt aatttcattg cttcagcaaa
     8161 cttggctact acacgttcat tggaataact ctgtttttca ggttgattcg gataaaaagc
     8221 ctgacgtagt tcctttaaat aattttcttg tggcaccacc ttcaacactt ctccatcttc
     8281 tgagagtaca cgttggaatt cttggtaatg agaaggcgaa aagatattta aaaccgcgtt
     8341 gaatgtttga tcagcaaacg gcaaattggt caaatcagct acacaccaaa atgcttcggc
     8401 agattgttct gtagctgcgt agactccttc ttttgaaata tcaaatccaa cagcattttg
     8461 aatctttttc aattttgaaa gttcattcaa gaagctacct tctccgcagc caacatctaa
     8521 tagcgagact tcttcaggta aatgctgcgc aatagtctca ataacgggct gatacatgcc
     8581 agattggatc attctacgcc ttggaacgaa cattccttga tcgtattctg ttttaaccgc
     8641 atgggataaa aaataaagtg ttcctttttt ggaaaggtca aaacggtgat tatttttgca
     8701 tatcaagcca ttatcagact tttttacttc ttcttggcaa accggacagc ggaacagctg
     8761 atgattttct gccaaaaact ggcgtcctcg gtcaattttt tttaacatag ttccccctca
     8821 tttctgtttt atcttaccgt tagatgctca ctttggcaac tgctaaaaat atgatacaat
     8881 gcctagaaga aagtgaaaga taaaggagaa gattagatga tcaaagaatt ttgcgctgaa
     8941 aactacacat ctattcctct agcaattgct agaggagccg atcggatcga attgtgtgac
     9001 aacttagcgg tcggtggcac aaccccgagt actggtgtga tcgaagaaac gttaagttat
     9061 gctggggaaa aagatatccc cgtaatgacg attattcgtc ctagaggggg cgacttcgtc
     9121 tataacgata tcgaattgaa aatcatgcat actgatttga ttgaagcaaa gaaattaggg
     9181 acggatggcg tagtatttgg ttgtcttact ccttcaggct ggctagacga agaggcctta
     9241 gaactattga tcgaaacagc agaagggttg cagatcacct tccatatggc ttttgacagt
     9301 attcctgaag atcggcaatt cgaagcaatc gattggctgg tagaacatgg tgttgaccgt
     9361 atcttgactc atggcggtcc tgctgacaaa actattgtag aaaatttccc acggcttaaa
     9421 gaattgattg cctatacaga tggccgctta atcattttgc ctggtggtgg tgtcactgca
     9481 gagaatgcag cacatgtagc taaagaattg aacgtaagcg aagtacacgg aaccaaaatc
     9541 gtggatcttc agttataatc ttataaacaa aaaaacattt gcaacaggca taatgcttat
     9601 tgcaaatgtt tttgtttttt tacataaaat agcccataaa taaagaagca atattgaaat
     9661 aaatcagtac actcgtcaaa tcgctcaaag tcgtgataaa gggaccgctt gctactgctg
     9721 gatcaaatcc tagacgatcc attagcatcg ggattaaact acctgcaaga ttcgccactg
     9781 tgattgcaaa tagcatagcc atccctatga caaaccccaa gataaaatta tgtttccaaa
     9841 aacctacgat tagaaaaatc gtgatccccg taacagctcc tgtgacaacg ccggtcaaga
     9901 cttcagccaa aacaagtcga acaaatccat gatctttctc atcagaaatc gcaagtcgac
     9961 gaacagccac agctagcgac tgggttccag cattaccggc agttcctgta atcagcgaaa
    10021 tgaagactgc taaaatactc gcttcactta ccaagtcttc atagtgactg attaaagaag
    10081 cggtcgacat acccaaaaac aacagagtga tcagccaagg caaacgtcga gacgctgcct
    10141 ttactggatt ttcattaatt tcttccacat tgaccccggc aagtcctgaa taatcgctag
    10201 cagcttcatc atcaataacg tcgatgatat catcgactgt gacgattcct aacaaatgat
    10261 catcataatc ggtaacagga agagctaaga agtcataatc tctaaatgtt tgtgccacat
    10321 cttcttggtc gtcgcctaca tgaactgaaa gcactcgttc gctcatcaga tcagaaatca
    10381 ttgcgtcgtc atcattcacg attaggtcgc gcagagaaat aacccctact agctgacctt
    10441 cttgattaac gacatagatg taatagattg tttcagctac atctgcttcg ttcttcagca
    10501 cgtacatcgc agagcgaaca gtttgattgg ctacgatcga aacaaattca gttgtcatga
    10561 tcgcgccagc cgtttcatcc tcgtaatgga gaagttcctt gatttcactg gcatcgtctg
    10621 ccgacatcaa acttaaatat ttagcaattt gctttttatc cagcgtatta agcaagtcaa
    10681 cagcattatc tgtatacatc tcagaaagca tatcagctgc atagctagga cgcatttcat
    10741 ctaggtattc tttcatatgt tcgttgtctt cttcaatcac atcgaacata tccgctaatt
    10801 ctttaggcga cagataggaa tagatcagct gcctgttctc ttctgtaaga gattggtaga
    10861 attgcccctg ctcatagata tgcatctcta aaaaactgtt tctgaatgcc tgaatattat
    10921 tttcccttaa atcttgagtc aactgggcaa atctttcctc taattcttga ttttcatcca
    10981 aaccaatctc acctcgcctt attcttcaaa ccgtctcaca actgcctcca tgtctgcggc
    11041 taaaggctgc ttgaactcca gcaactcttc tgtaaatgga tggtaaaaac gcagatcata
    11101 acaatgaagg gcttgtcttt ttattcccag atccattcgc ccgccataca tatcatcacc
    11161 gagcagggca cagccaattg cggaaaaatg aactcttatt tgatgtgtcc ttcctgtatg
    11221 cagctggata tcaactaacg cgatttctgt tagtcgcttc tctatccagt actcagtctg
    11281 tgccgaacgt ccttcatcag agaccattcg cttcaatagc gaagaataat ctcgggcgat
    11341 cggcaaatcg atatgtccat gtgccgataa atctgaaagc cttccactga ctaaggcctg
    11401 atattttttc acaaacttct tttcacgaag ttgcatatct aattttgcgt gcgcaaatcc
    11461 atgctttgca aaaagcatca aaccagatgt atcccgatct agtcgcgtca ccacatggat
    11521 tacttggttt tcgtaacctt gttttttata ataagccttt acacgattag ccattgtccc
    11581 atttggatgg tattgggcag gaatagacga aacgcctgct ggtttattta ctaccagaac
    11641 gtgttcatct tcatacacga tatcgatcgg tgtttcatcc aaaagtacgg tttcatgttc
    11701 tccttcagca ggaatcacaa tggttacctt atcatcctta gctagagaaa acaagacatt
    11761 ttctacttga tcattaactt tgatttgacc gccttgaaat tttattttcg ctaacagacc
    11821 ctttgagatc cctttttctt ttaaaaaata ttttacttgc tgagaatgat ctttctcata
    11881 atcccatgta aactgcatat caatcctcac taataaaggc atctttcaca cgatgccaaa
    11941 aatgcatatg tcgataagaa gcaaaatgga tccgttcttc tgctattcga taataaattg
    12001 agcggatatt tgcttttgcg acattcaatt gatcaaccgt tacaagataa tcttctgtat
    12061 tttccaattt tatttctagc cattcatcat gggcaatcac gatcggagaa cccaaagtac
    12121 gaaacactcg attattcagt gaagcaatct ctgccaactg aaaggcattg atactaggat
    12181 ggatcacagc accgccgaca cttttattat atgctgtcga accagtaggc gtagaaatcg
    12241 tcaatccatc tcctctaaaa ctttcaaaca gctcgttttt gatgtataca tcggctacca
    12301 tcgtacgatt ggcccgttta atcgttgatt cattcagtgc taaaaaatgc tggtccggtg
    12361 tttcatccaa ataactgatc cgaacatcca gcaagggata actaacactt tgttcccggt
    12421 tcgttcgtaa gctgtctact agttcttcca gctcgtaatc ccgccaatcc gtgtaaaacc
    12481 ctaaatgacc tgtatgcacg cccaagaaac gcacatcatt cagacaatga ctgtagtgat
    12541 ggaacgccga caataatgtg ccgtctccgc cgactgagat gaccaattct ggctcatgat
    12601 catctcgttg aatccctgct tgttctaaaa gtgcgttcaa acgttctgtc acttctatag
    12661 tctttgcttc tttattatga acgatcgcta ctttcatccc ctgcctcctc tctttcgctt
    12721 tctttcttca aaagcgttcg atagtagaca tcatctgatt ttcctcttcc atgggagaat
    12781 aaccgctgtg cttcttggat ttcttcacgg atactggaca tctcttcatc caactgatag
    12841 gcagcttctg ccgcacgctg aagtcgttca ctcatttctt ctggaaaaac gccttgatat
    12901 ttataattca gcgaatgctc aatcgttgcc cagaaattca tcgcaagagt ccgaatctgg
    12961 atttccgcca aaattttctt ctcaccgctg atcagctgta caggatactc gatcaccaga
    13021 tgataagaac ggtagccact tgctttttta tttgtgatgt aatctcgttc ttggagaact
    13081 ttcatgtctt ttcttttacg gatgatctca acaacttgat ggatatcttc tacaaactga
    13141 cacatgatcc ttaacccggc aatatcagac atttcttctt ctaaacgtcc cattggaata
    13201 tgccgtaatt gtgattttgt tacaatacta tcgactggct tgactctccc agtgacaaat
    13261 tcaatcggga catgtcgatt ttgttcacgg aactgtttgc ggatcccccg cagtttcact
    13321 ttcaattctg atacagtttg ttcatatggt gctaaaaatg cttcccaatc tcgttccata
    13381 gctgatacct ctacttcatt aacattcatt gtttattcta ccatatatgc aaagtttttt
    13441 cgtactcttg atttatgcat tgaccaaaaa aagaaaatta ggcaaaatag acaaaaagga
    13501 gtcgatcaaa tgacacaaat gcttgaaatc gaatataaat ctatgctgac aaagcaagag
    13561 tacgagatat tcatcgctca ctatcaatta acagataaag atttccgtgt acagactaat
    13621 atttatttcg ataccgttga tgaacagctg aagaaacaaa attgtggcct acggatccgt
    13681 ttcgttggtc atcaagctga atatacatta aaaacacctg ctgaaaaagg gcgtctcgaa
    13741 acaacggata cctttataac agaagaagca gaagcattta tcttcgaaaa acgtctgcct
    13801 cgttcaggcg cagtttttca aaaactttct gatttgaata tcgatcctgc ttcattaatc
    13861 caaaccggaa agcttacaac aaaacgggct gaattcccta ttgaagaagg acttttagct
    13921 attgatgaaa gctggggaga caatctacac gattttgaac tggaactaga ggtaggggat
    13981 gcatctgtgg gaaaaattgc ttttataaat tttttaaaac gtttttcttt accttatcgt
    14041 cctgctaaga acaagatcca acgtatgctt gaagctaaaa attagaaacc tttcattttc
    14101 acaaggcact tgagttcatt tttttacttt tttctgttaa gttataggat gtgaaagaat
    14161 ctagtcacta gttgtataga atctattttg ggggagattc atatgatcga aatctattta
    14221 tttgttaatc ctttagggga atactgtttc gaaatggaac aacagttatt acaatttatc
    14281 gaagaagagt acgggacacc atcaaaagaa aagatgcaat ttcgctttct tcctttggtc
    14341 aatttgcaga caattgggga tgtcatgcaa caaaaaggaa tttctcagaa caatcttgag
    14401 gaacgtaatc acttattctc aatgacttat tctgcagcat tggactgcaa agcggcgcag
    14461 tttcaaggaa agaaaaaagg ccgccgtttt ttgatcaaat tgcaagaagc agtcggctgt
    14521 aatgacatac catattctca agaacttgct gaatccatat ttgaagaaat cggcggcgat
    14581 ttgaatatgt ttaaagaaga tcgtcagtct gacttcgtta aagaagtatt cttttctgat
    14641 caaaaaattg ctcgggaaat gggcatcaca aaacaccctt ctgcagtcgt ttacaattat
    14701 gcctgcgacc gtgattttgg tgttttagta gaagtaaaag gtacaagtag cctttggaga
    14761 atcacagagc tgtgtaaaac aacagataac tcatatcaag tttttaatgg tgaacattta
    14821 catacaaatg attctcagtt ccattcaaga actagccatt tacacttgct ttctaattaa
    14881 aaaaatacgt cgactccctt acaagagagc cgacgtattt ttttcgtttc tattcgagaa
    14941 aatcaaaaat agaaagcctt ataaacgatc gatcaattct tctaattcat ttagacgttg
    15001 ttcaaacatc gacattgcat cttcaatata tgcagcctgt gtcatatcaa ctcctgcttt
    15061 tttcattact tccacaggat aatcgctgtt acctgctttt aggtaagcca agtagttctc
    15121 tagtgcctct ggttcctggt ttagtatttt cttcgccagt gctgaagcag cagaaaagcc
    15181 agtcgagtat tggaaaacat agtaattata ataaaaatgc ggtatccgtg accattcgaa
    15241 tttaatttct gggtcttctt cgactgctgg accataatat tttgcattca atttaccata
    15301 actatcactt aggtattcac tagtcaatgg cacacctttt tcatcttccg tatgcatgaa
    15361 atgctcgaat tcagcgaatt gtgtctggcg gaaaactgtt cctttaaacc catccaagta
    15421 atggttgagt acataagccc gtacacgagg gtctttttct gtttccaata aatactccgt
    15481 taggatattt tcattcgttg tcgaagcaat ttctgctaaa aagatggagt agtcgccgta
    15541 cacataaggc tggttcgaac gagtgaaata actatgaaca ctatgtccca tttcgtggac
    15601 aagcgtaaat agctgatcca gcgtatcatg ccaattcaat aaaatatatg gatttgtgtc
    15661 atagcttccc gaagaataag caccgcttcg tttcccttta ttttcgacaa catcgatcca
    15721 acgttcagag aatgcttttt ctacgatggc catgtattct tcacccattg gtttcagtgc
    15781 ttctaaagct ttttcttttg cttcttcgta cgtaaaggta attggagctt cccctaagac
    15841 cggtgtataa agatcataca tgtgcagttt ttccacttct aataaacgtt tccgtaattc
    15901 catgtatcga tgtaacaaag gcaaatgctt gtttaccacg tctaccaaag tatcgtatac
    15961 actttcagga atatgattat tgctcaaaga cgcttctctg gcagagctgt aattacggac
    16021 tttcgcttta aaattatgtc cttttatatg tgtgcctaaa gtagaagcaa atgtatttct
    16081 aaattgttcg taaacactgt acaatccctt aaatgctgct tcgcgcaccc ttcgatccgt
    16141 gctttctagc aactgaccat acacgccatg agataattgg actatttcac cattttctcc
    16201 ttcaatcgtt ggaaaaacta gatctgcatt attcaaaaca gcaaatgtat cacttgaagc
    16261 atcaaagatt tcacctgctc cagcaagaag agattcctgc tcagcagata agacatgggc
    16321 tcgattatct actatttgct ggatataatg gcgatagact tccaattttg gttcttcttt
    16381 gaaatattgc caaatctggt catctgacaa ttgcaatatt tctggttcaa accaggaaac
    16441 ggcttcacta actttcgaaa acagactgct tgctcttgca taaagcgctt ggtaatctgt
    16501 atttccagtg tcttgatcgt ttttaaggtg cgcatataca taaatgactt cagtttggcg
    16561 ataaaccctc aatacgaatt caatcgcatt taaaaattga gaagcgcctt gatcaagtgt
    16621 tcctttgtgt ttttcagatt gttttaactc ttctgataat tccaaatatt tctcatcaaa
    16681 ctcttggtcg cttgaaaaga tcttggtcaa gtcccaagtt aaattttcag gcaattcttc
    16741 tcgttttggc aactgcttta cttccatgaa atccactcct tttcttccct tagctctatc
    16801 tatcaaaata gatcatagtc taacatccac atgtcacagc atctaacttt tctactcatt
    16861 catgtgtctg cttccatcaa ttttagcata actaaatctt gttttacata gaaaaccgat
    16921 tcctagccta tttttatact tactattttt tcaaagaaga caagtgtatg cattcgttaa
    16981 aaaactgctg atagaccatc tcttcatcaa tcataggaaa aagccattcc cttttatgtt
    17041 cttttatttg gcaaagccat gagttgaaat caggaacttt ctggttttcc aaggcttctt
    17101 cgtacaacat tcgataaaga aaaacctgtt cttgtaaata aaagaaaaac cgactttttt
    17161 gatagatcca cgatgataga tgcaaaagat gtttatgacg caagtagcat tgttcttgta
    17221 tggatactgt attcttttgc ttgtgaataa gttggcgtgt cagccatttc ttcccatctt
    17281 cactcacttt ttctattttt ttcatttggt tcattttaat ttcgttacta gtgaatattt
    17341 ctttaaggtc cctactagaa aaagtccagc agctactgca aaaggaaata tgccctgata
    17401 cagtctctgt aatatgatga tacaaataaa gcttttgttt cttgagatca gctttccagc
    17461 aatgcactcc tctttttcta ttatagtagc agcaactttt ttctatcaat gagaattgcg
    17521 attgtccaag aaaagaatta ccaagtatcc accatacttg gtaactgtta ttcaaataat
    17581 tttgggttct ttcaataaat ctctggtgag acagtctaga acattggatt tctattgcta
    17641 gattcgccaa caaaatatcc ggcctttgcc gcaacctcgg caaataggct tccaaaaata
    17701 ctggttctac tgactgtccc agccaatcca tgaagcattc ctttaactgt aagtgctctt
    17761 ttgattctct ttcactaaag accgaacagt tatctgattt ttgatgggca aaatgagcac
    17821 attttttctt tccctttttc aaaatcactc tttttcgaca gccacgacaa taatatttct
    17881 ttcctttttc tgcatgttta gcaaaacagc acttattata ttcattcatt gcaagcagca
    17941 tttttctccc tctgaactta tttacgtcta agaatttatt tacacgaaaa aagagggcgt
    18001 gaaaaagcta ttctgcatca agtaataaga acagctaaac aaaaatagtt cccaatattt
    18061 ttcgtttggt tgggcttgtt accgcagatg caagcttttg aacacattta tacgaaaaaa
    18121 agactgcgac acaatttttg tcacagtctc ttactataat gctgatttat acaaaataaa
    18181 atttcgttcg ctcgatagca ttacgcgcca tcagacattg tccatattca ttcaatgttt
    18241 ctggtgtacg atcgctcttt tcagcaaact ccaaaattcg agcaatctga ttctctacat
    18301 ccgcttggtt gagattttcc atccagaagt gaacgactag gtaatagcgc tcttcgaata
    18361 aatacaaatc agtccaagca gactctagtt cgacatctgt tgcaagttgg atcacttgtt
    18421 caaaatcttc aaaagcaaaa gttgcttgtc tttcctgttc ttctagggcg cttccagata
    18481 attcttcctc cggatcgctg ccaataatct gctgacgaag aaagtcactc acatcttctg
    18541 aatcaacagt agaatcttct gattgagaaa tttcttcatc cggaagattt ttactgataa
    18601 acagttctaa tccgtctcct tttggcaaaa cttggaaagt gacagcttcg cttcctcgga
    18661 actcctcttc gatatcaact tcttccaaaa tactataaaa gaaattttcg atttctttgt
    18721 gattgccaag taaatccaaa aatgaaacgc cccgttcctc taaatcttca tgaccaatta
    18781 gtacacggat cgtattttca ttaatatgtt ccatttccat tttgctacac ctcactttgc
    18841 cacaatgttt aatatagatt cattgtagcg aaatcagctt aaatgtaaag aaaaaaaatc
    18901 tcgctgttaa gcgaatttaa taaaaccagc ggatttttca acctttcaag ctatcttatt
    18961 taactataac atttttggca gacgaatact atatagccga cttttcagct aattttccct
    19021 ctctattttt cgtgcaatca tcatcttcta ctaccttttt atacatctaa tcaagaatac
    19081 tttttgtaaa aacaaaaaaa cttggaaaaa ctatctttgt aatagtcttt
//
LOCUS       DARBEC010000056        18741 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.057, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000056 DARBEC010000000
VERSION     DARBEC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.057"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            257..532
                     /locus_tag="SCL80_001822"
     CDS             257..532
                     /locus_tag="SCL80_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107683.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            545..2617
                     /locus_tag="SCL80_001823"
     CDS             545..2617
                     /locus_tag="SCL80_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEG1107684.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKRVQ"
     gene            2782..4668
                     /locus_tag="SCL80_001824"
     CDS             2782..4668
                     /locus_tag="SCL80_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEG1107685.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            4680..5627
                     /locus_tag="SCL80_001825"
     CDS             4680..5627
                     /locus_tag="SCL80_001825"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEG1107686.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            5647..6174
                     /locus_tag="SCL80_001826"
     CDS             5647..6174
                     /locus_tag="SCL80_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1107687.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(6237..6890)
                     /locus_tag="SCL80_001827"
     CDS             complement(6237..6890)
                     /locus_tag="SCL80_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEG1107688.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            7022..7864
                     /locus_tag="SCL80_001828"
     CDS             7022..7864
                     /locus_tag="SCL80_001828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1107689.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            8022..8918
                     /locus_tag="SCL80_001829"
     CDS             8022..8918
                     /locus_tag="SCL80_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEG1107690.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            8921..9634
                     /locus_tag="SCL80_001830"
     CDS             8921..9634
                     /locus_tag="SCL80_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEG1107691.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            9654..10172
                     /gene="msrA"
                     /locus_tag="SCL80_001831"
     CDS             9654..10172
                     /gene="msrA"
                     /locus_tag="SCL80_001831"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEG1107692.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            10169..10396
                     /locus_tag="SCL80_001832"
     CDS             10169..10396
                     /locus_tag="SCL80_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEG1107693.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            10548..11099
                     /gene="lepB"
                     /locus_tag="SCL80_001833"
     CDS             10548..11099
                     /gene="lepB"
                     /locus_tag="SCL80_001833"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1107694.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(11182..11682)
                     /locus_tag="SCL80_001834"
     CDS             complement(11182..11682)
                     /locus_tag="SCL80_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEG1107695.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(11961..12359)
                     /locus_tag="SCL80_001835"
     CDS             complement(11961..12359)
                     /locus_tag="SCL80_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1107696.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            12619..13479
                     /gene="ylqF"
                     /locus_tag="SCL80_001836"
     CDS             12619..13479
                     /gene="ylqF"
                     /locus_tag="SCL80_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEG1107697.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            13472..14239
                     /locus_tag="SCL80_001837"
     CDS             13472..14239
                     /locus_tag="SCL80_001837"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEG1107698.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            14295..15152
                     /gene="dprA"
                     /locus_tag="SCL80_001838"
     CDS             14295..15152
                     /gene="dprA"
                     /locus_tag="SCL80_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEG1107699.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            15274..17352
                     /gene="topA"
                     /locus_tag="SCL80_001839"
     CDS             15274..17352
                     /gene="topA"
                     /locus_tag="SCL80_001839"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEG1107700.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            17321..18697
                     /gene="trmFO"
                     /locus_tag="SCL80_001840"
     CDS             17321..18697
                     /gene="trmFO"
                     /locus_tag="SCL80_001840"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEG1107701.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
BASE COUNT         6518 a         3231 c         3899 g         5093 t
ORIGIN      
        1 tttttgtcgc agccccattt ttttgttgtg ataaaagaac aagaaaggca ggaatgggaa
       61 agtaaacatc cttttgtcag aaacttttat ctttcttaag ttttacttga tatctgatac
      121 tatttggaat agaatgaggc gttgaaaaat ttatggaaat ttctaactag caataaacga
      181 tgaaacaatg aaatattttt catccaaaaa gaactttgct ataatgatgg aagtttgtgt
      241 ataggagatc tggaaaatga gagagagttt tgaacaacaa aaaaaactgt tgcatgatcg
      301 ctatggcgcc ctttcgatgg atgaccgccg tcaaatactt tgtaagttgc gtaaaagaaa
      361 tatattgatg tatcgtcagt tggaacgtct gaaacatgat ctgcttcgtt tggagtcaaa
      421 acgtgtacag tgtgaattag aggggaacca aacacaggtt gaagtagtcg aaaccaaaat
      481 tttaaagaaa aaagaacaat ttttaaaaat gcttacacaa aataaaaaat aaaggggatg
      541 gacgatggaa tttgctgagt tagttattgt atttgcagta acgatcacct tatcgaacat
      601 agctagccgc attttgccaa tgattccagc tccactgatc caaattttcc ttggagtggt
      661 attaggtctg acagagtggg gacaatcaat cgattttgaa cctgaattgt tcttagttat
      721 gattattgca ccattgttat ttagagaagg agaaaaagca gatatttctt cgattctcaa
      781 aaatttcggg acaattttgt tcttggcatt tggaggagtc attttgaccc ttgtcggggt
      841 aggtgcgacg ttatcattcc tgttgccaag tgtcccactt gctgcctgct ttgcttttgg
      901 agcagcgtta ggtccgacag atgcggtagc agtcagttct ctttctggac gagtgaatat
      961 ccctaaaaag gcgatgcata ttttggaagg agaaggtctt ttgaatgatg catctggtgt
     1021 tactgccttc cagtttgctc ttggtgcgct gatcaccgga tctttttcag cagtaaacgc
     1081 ggggatgagt ctagtagttt ctagtattgg gggagctttg atcggctttt tgcttgtctg
     1141 gttcaaacaa aaaataattc atttgatcga aaaagcatca gcacaagatg tcacagggta
     1201 tctgctgatc gaacttttgc ttccttttct agcctatgta ttggcagagg catttgatgt
     1261 atctggaatc attgctgcag tggcagcagg tattctccaa gcttctggtt ttaggaaaat
     1321 ctctgtgttt gatgcagaat tgtcgagctt gtcccacagt acatggacaa ctattgcttt
     1381 tacactgaat gcattagtat ttatattttt aggaatcgaa ttgacgcaag tgttctcgcc
     1441 agtatgggga gacggtttat atccaaacgg tcttttatta gcgattatcg tattaatctc
     1501 tgtcatgctt tttgtgatca gattcatttc tatcagtctt ttttatgtat tcaaagatgg
     1561 cagtaaaaag ttcaaaaaac agttgaacga gatcttgatc ttaacatttg gcggtgtaaa
     1621 gggaacagtc agtttggcta cgatttttat tttgccattt tcaatcaata atatgatgtt
     1681 ttaccagcgg tctttgcttt tgtttctgac ggcgggtgtc atcttagtga ctttggtcat
     1741 agggatcatt gtcttgccta tgctgacgga aacagaggaa gcgaaaagca cggatttgaa
     1801 tgctttgatg attttagaag aggtcgttga agttttgcga aaagaaataa aggaaattga
     1861 tcgtaataca aaagaatttt tagccactga agcagtgata gaaaattatc aagaacggat
     1921 acgtgatctg tacttggaag atttaaccga tgatgaaaag caagaagtac aagaaataca
     1981 agcactgatt ttgtcgatcg aacgggatgg attggatgaa agttatcgtt cgggcaagct
     2041 ttcttctaat ggttatcgtt tttactcccg ttttctttct cgattcgaac attctatcac
     2101 aagccagatt ttgtctttta tcggcttttg gttcattgtt gtccgtaggt taatgcgaat
     2161 cgttctccat ccgaaaatgt tttggcaaag aagatatgct gatcgtcaga catttattag
     2221 tgatgaagat atccaagaaa tcagagagac atatcaaaaa aatactcagt tgattattga
     2281 aagtttagat aacttgactg atatttatga tgaaacgatg gttcaatttt ttatccagca
     2341 acgcaaactt gaaggaataa aaatgaactc ggggaatttg atttcttcgt ggatgatcca
     2401 gcaagatagt ctgtttacga aaaaaatgct aagaggttat tatcttgaaa gaaaaatcat
     2461 tgatgaatac gaagttgctg aaaaaatcac gacgttttct gcgaacagct atcgcaggaa
     2521 tatcaatttg ttagaatctt atacgatgaa caaaccatct gataactttt ctttccgatt
     2581 tgcgtttcga gcaaagtcaa aaaaacgagt ccaataaaat aaaaaggtaa aaattttcaa
     2641 gttttataaa aaacaaaaca tatcagaaca ggatcgaatc ggtcctgttc tttttttgag
     2701 aacacaaaaa gaacaaatac caaaaaaatt ttcctgaagt tcactatttt tatgatacaa
     2761 tatccaagat aaggagggaa catgttgaaa gaattaaaag taactgacct caaaaaaact
     2821 tatggtgaaa aagatttgtt tgaccagatt tcttttttgg tccacgaaaa agaccgtatc
     2881 gggttgattg ggacgaacgg gacgggtaaa acaagtctct taaatattat tgccggtatc
     2941 gatagcagcg acggtgatcg gcaaacagtg ttttatccaa ctgattaccg tattggttat
     3001 ctttctcaag cagctgaatt ttccgatgaa ttaactgttc tccaagcagt ctttcaagga
     3061 aacagcccct tgatccaaac tgttcgagca tatgaggaag cattgatcga actaggggaa
     3121 aacggagaag accctgatgt gcaaaaacgt tatgctaaag cagaagaaca aatgaataaa
     3181 gaagatgctt ggactacaga taccaacgca aagattattt tgcaaaaatt aggaatcagt
     3241 gaattagata aaaaaatcag tactttatca ggtggtcaga aaaaacgagt tagtctggcg
     3301 caagtgttga ttgatgaacc cgatttacta ttactagacg aaccaacaaa ccatttagat
     3361 tatgaagcga tcgagtggtt ggaaaactat ttgaaacaat accgtggcgc actattgatg
     3421 gttacccacg atcgttattt tctagataga gtagcaaacc gaatcttcga actttcattt
     3481 ggaaaattat acgagtataa aggaaattat gaagcttacg tcttagaaaa agcagaacgt
     3541 gatcgtgtag aagtagaaca agaagaaaag cgcaagcggc tttataaaca ggaattagct
     3601 tggatgaggg ctggcgtaca ggcacgtggc accaagcagc aagcacggat caaccgtttc
     3661 gaagatctaa aggagaactt gtaccaagta aaccaagaag atgatttaga gctgaaccta
     3721 gcgacacaac gtttagggaa aaaagtactg gaaatcaaag atggcagtta ccgtataaat
     3781 gaccaaacac tgctagaaca tttagattta ttgattcaat ctcgggaaag actaggtatt
     3841 actggtaaaa atggtgcggg aaaatcgaca ttgcttaata ttctagctgg cagaattccc
     3901 ctggatagcg gtacaatgtc tatcggtgaa acggttcatc ttgcgtatta tacacaagaa
     3961 aatgaagaaa tggcaccaga taaacggatg attgcttact tacaagaagc tgctgaagaa
     4021 gcaaaaacag cagatggcag tcaaatcgga gtagcagaat tacttgaacg atttttattt
     4081 ccaagattta tgcatgggac actgataggc aaactttcag gcggagaaaa acgccggtta
     4141 tttttgttga aactgctgat tcagcaacct aatgttttat tattggatga accgaccaat
     4201 gatttggaca tagcaacatt gacgattttg gaagattatt ttagaagttt tccaggtgct
     4261 gtgatcactg tttcccatga ccgttatttc ttggacaagg tagcagataa gttattagta
     4321 ttccaaggaa acggaaaaca agaactttat tacggaaaca tgtccagtta tttactgaag
     4381 caaaaagaga cccagcagcc tgctgaaaaa gcgaaaccaa aaatacagag caaggaacct
     4441 gctgaaaaga agaaattatc ttacatggaa caaaaagaat gggaaacgat tgaagatgag
     4501 attgcagaac ttgaagaaaa aatctcattg ctccaagaag agatgaatca tcaaggagat
     4561 aatttcactc gattacaaga attgcaaaac gacgtatctg aaacagaagc acaacttgaa
     4621 gaaaaaatgg cacggtggga atacttgagt gaatgggtag aagattagga ggaaaaagga
     4681 tggaacaagc gtatcttgac ttaggaagaa aattgttgga agaagggcat cgtaaagaag
     4741 acagaacagg cacaggcaca ttaagcttat tcggatacca aatgcggttt gatctgcaaa
     4801 aagggtttcc tttgttaaca acgaaacgag taccttttgg attgatcaaa agcgaattgc
     4861 tttggttttt aaaaggagat acgaatatcc gctatctatt acaaaataaa aaccatattt
     4921 gggatgaatg ggcatttgaa cgctacatca aaagtccaga ctataatgga ccagatatga
     4981 ctgattttgg ccatagatgt ttgacagatc ctgaattcaa tgaagaatat aaaaagcaga
     5041 gcaagctttt ttgcgagaag atcctaaccg atgattcctt tgctgaaaca tacggcgatc
     5101 ttggacacat ctacggctat caatggcgtc actgggaaac aaaagacggt ggctttatcg
     5161 atcaaatcaa acaagtgatc gaagcaatca aaaagactcc tgattctaga agattgatcg
     5221 tttctgcatg gaatccggaa gatgttccta gcatggcttt gcctccttgt catacgatgt
     5281 ttcaatttta tgttcaagag ggacggctaa gctgccaatt gtatcaacgc agtggggatg
     5341 tgtttctagg cgttccgttc aacattgcaa gttatgcgtt gctgactcac ctaattgcac
     5401 atgaaacagg tttagcggtt ggcgaatttg ttcacacatt aggagatgct catctttata
     5461 tcaatcatat cgaacaaatg aaagaacaat tgagccgaga aatacgcgca tttccgactt
     5521 tagttttgaa tcaggaaaag cagtcagtat ttgattttga gatggaagat atccaattag
     5581 aaggatatga cccacatccg gcgatcaaag ctcctatcgc tgtttagaat cagaaaggat
     5641 gagaaaatgt tagctgcaat ttgggcacag gatgaaaacg gattgattgg aaaagaagac
     5701 cgtcttcctt ggcgtttgcc aaatgatctg aaattcttca aacaaatgac agaagcaaat
     5761 acactagtga tgggacgaaa aacattcgaa gggatgggta gccgtccgtt gccgaatcgt
     5821 cagacgattg ttttgacgag agattcttcc tataaagcag aaggcgtgca tgtcatgcat
     5881 agtgtggaag acgtgcttgc tctggaaaaa gaaacagacg gtatcttttt catcgctgga
     5941 gggtcagctg tttatcaaga atttctgcca ttttgtacga tattataccg tacagtcatc
     6001 catcatgtat tcgatggaga tgcttatttt ccacctgttg actggtctga ttggtcactg
     6061 atcaatacta gtcaaggaga aatggatgaa aaaaatcgtt atccgcatca atttgaaacg
     6121 tatcagcgta atgagaatcc tgtaaaaagc gggacaggcg aattgacgat ataaaaaagc
     6181 tatggataga ctagctgtta tttagagctg tcccatccat agctttattt tttttattaa
     6241 gtaaagaata aaacagagaa gtacataaaa cctgcaccta acataacaaa caaatgccaa
     6301 acaacatgca tatagcggat agaattcaag ctataaaaga atgcgcctat cgtgtaagaa
     6361 agcccgcctg ctaccatcaa aataacacca gtcattccaa gagagtgata aagaggaaca
     6421 gcagccacga cacagagcca ccccataaaa atatagatga tcgtagatac ttttgatact
     6481 ctctcccgtt tatgcaacgt caacgcttta tatataatgc caagaatcgc aaacaaccag
     6541 attaatacaa ataaagtcca acctagccat cctcggatac tcagcaaaca aaaaggagta
     6601 taacttcctg caatcaaaag aaaaatcgaa ctatgatcga atacttggaa cacctttttt
     6661 gcttttgtaa aaatcaatga atgaaacaaa gtagacgtta aaaataaaag aatcatcatc
     6721 gatccataga tagcataaga gacaatatgg agggaggaat gcaagtgggc accttttact
     6781 aaaagaacca ctagtcccgc gatactcaat ccagtcccaa ttccatgggt gactgcatta
     6841 aacacttcat ttaatatttc atacgttcga ttaaatttcg ttttttccat gaaaacggct
     6901 cctttactag gtgtaattaa tgaaaaatca ttatttgtct gttatactaa aaatagaaat
     6961 tgtcttacat gtagtttaac atgaataagg catcttgtta aagaaagaga gagagaaaat
     7021 aatgacaaat gtaaaaatcg taacagattc ttcatgtacg atggaaaaaa atgtacgcga
     7081 tgctttaggc attcgtgtca tgccgctatc catcatgatc gacggaacag tatatcccga
     7141 tgatgaccaa ctagacggcg aaagatttat ggaaatgatg gcgaaagcaa aaaatctgcc
     7201 caaaacaagc caaccaccaa ttggagaatt catctcgatg tatgatgagc taggagaaga
     7261 cggcagtgaa gtgatttcga tccatatgac aaaaggcttg agtggaacag tagaagcagc
     7321 aagacaagcc agtaatctaa caaaaacgaa agtaacggtt atcgacagtg ataccactga
     7381 ccaaggatta tcttttcagg taattcgagc agctgaatta gcaaaagaag gtaaaacagt
     7441 agagaaaatc cttgaagaag tcaaaaaagt acaagacaat acaaaattat acatcggaat
     7501 atccacacta gataacttgg taaaaggtgg aagaatcagc cgaacaactg gtttgctgtc
     7561 tagtgttttg aacatacgcg tggtgatgaa tttcgcccat tctgaattaa tccctgtaac
     7621 taaggggcga ggaaagaaaa cgttcgataa atggtttaat aatttgaaaa atgaactgaa
     7681 agaattgcca aatgtgcgac aaatcggtat ttcacatgcg gatgcaaaag aattggcgca
     7741 agaattcaaa gagggtttgc aagaattatt cccggacatg catatccctg tactgcatac
     7801 gaatccggtt atcgctacgc atactggtaa agacgcgttt gcaatcatgt attatgtaga
     7861 ttagtcgaaa agaggataag ccctcagcct gaaaggcttg cagcttattc tctttttctt
     7921 tataaaatta ggaaaataat tgttttcttt tttcataaag actacctttt tgataaaatt
     7981 tttgataaca ttagaaccaa tcgaatacaa gaggtgaaaa aatgaaatta acgaaacgac
     8041 atttgatcct cgttttgttc ccattattaa ttgcgatcat catctatgca ggtttagctg
     8101 cttctatacc gaaagctggc ccactaatga aaccagtaac tactgtgaga gaagatccaa
     8161 ataaaaaaga aaaacttcac tacatagcta tcggagattc gctgactgaa ggagtaggag
     8221 atcaaaccaa gcaaggagga ttcgttcccc ttgtcgcaaa tgatctaaaa gaccgttata
     8281 atttgaccgc ggttgaaaca gaaaactatg gtgtaaatgg tgaacgaagt gaccagatct
     8341 taaagcgagt aaaaacaaaa gaagaaattc gaaacaatat caaaacagct gattttatca
     8401 cactgactgt tggcggtaat gatttgatga aagttatcca aaataattta ttcgggttaa
     8461 cggtcaattc ctttaaaaag ccactgaaaa actatcagga aaatgtcaca gaactattgg
     8521 aagaatttcg atccctcaat aaaaccgcac ctatttacat tttaggcatc tacaatccat
     8581 tttatttgaa ctttcctgag atcactgaca tgcagacaat tgttaacaat tggaacaaag
     8641 gaacagaaac ggtgacaaaa aaaatgccga actgctactt tatcccaatc aatgatttgc
     8701 tttatcaagg attgaatcag gaagtaggtg tcacagaaga aggaacagat caaacaacta
     8761 gcacgacaga aaatctaaat ggcatcaaaa ataatgtgct ctatgaagaa gatcatttcc
     8821 atccaaataa tttaggttat caattgatgg caaatgcagt gagagatcag ctgatcgaga
     8881 cacaaaacca atggttagag aaggaggatg atcaataaac atgaaaacaa gatctgacgt
     8941 atcttcaaac aaaacaaaga aaacaaatta ctggaaattc gccttcctta tccttcttgg
     9001 ggtagtactt ggttcgaccg tatttttagg cagccgtatt tttgctaatc gggaaccaga
     9061 actcccagaa ataccagctc ttacggaaag acaaggagat cctgtcttaa cgatcaattc
     9121 aaataaagaa aaagtcaatc aaatcatcag ctttttcctt agtgagtatc aaaaagatag
     9181 tgatattgaa tataaatttt atttggaaaa tgaagcacta ctcaatggta ctttcgaagt
     9241 actaggtttt cctatcgatt tctatttata ttttgatccg tatgtgatgg aaaacggaaa
     9301 tgtccaacta aaagctagaa gtctttctat cggaacattg agtttgccaa tcaaagatgt
     9361 gatgaatatg atcaagagaa actataaatt accagattgg atcgaagtca atacagatga
     9421 tctgactatc atgcttaggt tggatcaatt ccgaatgcaa aacgggatgt atattaaagc
     9481 agataaaatc gacttagtaa atgatgacat tcggtttagt ctatatttgc caaaagatac
     9541 ctctgatgct tcaaacaact caaataccaa caaaactagc accagcagta ctgaaagcaa
     9601 gacaaaagaa aataaaaaat catcggatga gtagtattag agggggaatc ataatggaac
     9661 gagcaatatt tgcaggaggc tgcttctggt gtatgatcca gcctttcgac acgcttcctg
     9721 gtatccatac agtgatgtca ggatatactg gagggcatgt accagatccg acttatgaac
     9781 aagtaaaaag ccaaacgact ggacatacag aagcagtgga gattatctat gatccaacat
     9841 tgatttcata tgaacaatta gtagaacttt actggcagca aactgatcca actgacgctt
     9901 tcggacaatt cgaggatcgc ggtgacaatt atcgcccggt gatcttttat ttgacagaag
     9961 aacagcggat cattgctgaa aaaagtaaga aacagttagc tgaaagtgga cgtttcgaac
    10021 agccaatcgt tacaaaaatc gaaccagccc aaccatttta tctagcagaa gaagaccatc
    10081 aagagtatta taaaaagcat cctgaaaatt ttaaaagaaa ccatgcacgc cgggctgcct
    10141 ttatcgaaga aaattggagg aacgaagggt gaatcgaagc ttttttcatt atttaatgac
    10201 actgcgaggc ggtggcacac atgatgcttt atctgttttc gctacagaag ccggtaaaga
    10261 catccaattt cctaagcaca gctctagcta cgaagaaatt tctaattatc tggaactgaa
    10321 tgtcgactat ttgccgtcca tggatatctt tgacgaagca tgggaaaaat atttagaaaa
    10381 taatcatgac atataagaga tagttttgta ctttatgatc aaaaaagttt tttgtccaat
    10441 agcctaattg aaagaattat ttttatttca gctatctagt cgcatttttt tgatattttt
    10501 cttataataa agaaagaatg aaaaaaagag ggatggattg atagagattg acaaaaaaac
    10561 aacgctatat taatcgcttc tggcttgtat tcaaatactt acttgtttct gtattccttg
    10621 cgttcatgtt gcggggattt ttattcatcc cggtaccagt ggaaggcaat tcaatggaaa
    10681 acgtgttgaa gcaaggtgat atggttgtga tggaaaaatt ttcggagatc agacgatttg
    10741 atattgttgt ttttcaatta gcagatggca cgatttatat caaacgagta atcggtcttc
    10801 caggagaaaa tgtcagctac caaaatgatc aattgaagat caatgggaaa gtagttaagg
    10861 aaccttatct aactaaaaac ctgaaatcag atcatgcaaa tgcatcctat acgacggact
    10921 ttacactaca agagctaacg ggacaatcca agcttccgga agataattat tttgtacttg
    10981 gagataatcg aagagtttca aaagatagtc gttcgtttgg cacgatcaat aaaactgata
    11041 ttctaggtaa agcaagattt gtctattatc cacttgatga aataaaatgg atccagtgag
    11101 aaacaataaa gcgtgcagtc tgttgagact gcacgcttta ttgtttaatc caaaatcagt
    11161 ttactacctt agtacgtaaa atcaaacagc tcgtttaaac acttttctag cctctaaagt
    11221 tttgataaat gaataaacaa ccattatttg aatagggatt tggatcatat tcctgatgaa
    11281 tctacctaga aaaattactt tgaaatctgc accaaacaaa acagacatca gccaagaatt
    11341 gataaaaagg ctgactggga tcataatcaa taattgggca gcgatgacgg aaggcaggtt
    11401 tatcttttta tgatataaaa ataagccatt aataaatcca ccaaggaaag ccgtgataag
    11461 aaaagcagct tgaaacgttc ctgaaccaaa tatcgtgaaa tttaaagtgt tcgacaacat
    11521 ttcaagcaaa ccagcccaga tcggtccata tagataaccc ataagcgcca tacttaaaaa
    11581 tccgaatgtg atttgaaaat aagcaggacc aatcgctatc ttgtataaga tgacatcaag
    11641 ggctaataaa agagcagagg tcgacaacaa tcttgcattc atttcgggca atctaaaaac
    11701 gtcttttgca atcaaagtgt tcaaaatatt ctctcctttg tcagttaata ataaaaggat
    11761 accataaaat caccaatgtg tcatgacaca ttggtgattt acatacactg gcatctaaca
    11821 attaaaaaaa gggtgagaag actgctttta taaactgtaa aaaagagatt gtacgaataa
    11881 aaaaaataga ggcggagaat acttatcgta tcatccgcct ttgttttttt gttataaaaa
    11941 acgtcagatt agttatctgc ttacatcttc atccatacgg gttctaatgt atttccgtct
    12001 agatcttgga cttctaatga aaacatttct tcttcaggca ttcccatatc tacttcgtgg
    12061 aaagtaccac cattttcttt tgcaatttga gcaaattttt ttacttcatc cgcgttttca
    12121 acactaaacg aaatcaaagc accacttgtt tttttattat cagctatttc tttatctttt
    12181 ataaattttt tataaaaatc atgagagaga agcattatcc aaaaagtgtc atcccatacc
    12241 attgaaccag cttcttcagt agaaaattct tcattttttt taaaacccag cttttcataa
    12301 aaagcaacgg aacgattgac atcagcaaca ggaaaattaa caaaaaccat actattcatt
    12361 tgtttcgtct cctttctgtt ttgttcgtct atcatctctt atttaggaaa cgctgtcaaa
    12421 aatttagatt catatataga aaccaaaata aaaatttgca tcatcaaaaa agtgaagatg
    12481 tgatagaatg aggactggtt cactttcact taactaatag atggaatttc tttataataa
    12541 aaaagtgaat ttatattgaa aaagaaaaaa acaagtaaag ccggggaata ttccggtatg
    12601 aaaggaatgg agagaacaat gaccatccaa tggtttccgg gacatatggc aaaagcccga
    12661 cgagaagttt ctgaaaaaat gaaatatgtg gacatcgtat ttgaattggt agacgcacga
    12721 ttacctttat cttctcgtaa tccaatgcta gatcagatca ttcagcaaaa acctcgcctt
    12781 gttttattga ataaagcgga tctggcagat ccacaacaga caaagctgtg gcaagaatat
    12841 tttcgtaaac aaggccatct tgcattagct atcacggcac aagaaagtaa aggaattaaa
    12901 ggtctagtac ctgcggctaa agaagcattg aaagaaaaac gggcaagaga tgctgctcgg
    12961 gggatcaagc ctcgcgctat tcgggcaatg gtcttaggta ttccaaatgt tgggaaatct
    13021 acgttgatga atcgactagt aggcaagaaa attgcacaga caggaaataa gcctggcgta
    13081 acaaaaggtc agcagtggct tcgtttaggt aatgagttgg aattgttaga cacaccaggg
    13141 attttatggc ccaaatttga agatgaagaa atcggcaaaa aactagcgct gacaggcgcg
    13201 atcaaagacc agcttgtcca tttagatgat ttagcgattt acggattaga cttttttgcc
    13261 cgatattatt caggaagaat cagcgatcgt tatggtttat caatcgagca agaagaaaaa
    13321 ttggcaccgg aattactgat ggatattaca gcaaaacgtg ggtataaaga agactatgat
    13381 cgtggcagcg aattagtcgt gcatgatatt cgtcaaggaa aacttggacg atacacactg
    13441 gatcgttgcg aagaaatgga gcaaaaaagt gatgactgaa tccatccaaa gtatcaaaga
    13501 acgattgaaa agcatacaga gaatagatga tcctacacta gaatcattaa gaaatgatcc
    13561 acgcaaaggc gtgcagcaag cactggcttc ttttgaacga cgattgaaaa aggaagcatt
    13621 aattaaggaa aaatatgaag aaatgttttg ctttgaaaag caagggtacg cacaaggtca
    13681 ccaatggatt gccggcattg atgaagtagg aagaggacca ttagctggac ctgtcgtggc
    13741 agctgcagtt atcttgccga aaaatgagat catattaggg ctgaatgact ccaaacaatt
    13801 atcagagaaa aagcgagaac ttctttatca acaagttcaa gaaaaagccc tggcaatcgg
    13861 gatcgggatc gttgatcagc agacaatcga tgatgtgaat atttatgaag caagtaaatt
    13921 agcgatgatc caagcagtag aaaaattgtc cattgtacca agctatttgt tgatcgatgc
    13981 gatgaccttg gatttgccta ttcagcagga aaaaataatc aaaggggatg cacgatctgt
    14041 gtcgattgct gctgcaagta tcattgctaa agtatatcga gaccaattga tgaaagaata
    14101 tgacgtgctt tatcctggat atggtttctc gcataatgca ggttatggaa caaaggaaca
    14161 cttagaagga cttgaacgac aaggaattac gccaatccat cgtaaaagtt ttgcacctgt
    14221 aaaaaaatat ttacaataaa aaacagccgc ttttacgtat cttattagaa agagagacga
    14281 aaggcggttt tatgatgtat caaatagaag aaaatttatt gaaacttgct tatacaaaag
    14341 gaatcagtag tcaaggaaaa tgggcacttg ctaactggat gatgcagtat ccttataaag
    14401 aaatcggatt tgaagacatc attaagatag gtaaaataac caaacatcgg gagctgtttc
    14461 gcaaaagttg gcaggaaatt cacgctaact ggggcaaaat ccaatcgaaa cagtcgttta
    14521 tcacttgctt tgatcccaaa tatccccctc agctgcttca cttgacctat ccgccaattg
    14581 tactgtttta taatggtgat ctatctttac tttcttgtaa catgttaagc gttgttggtg
    14641 gaagacacac ttctgcttta gccaaaaaaa cagtttatta tttactaaaa ccagtgatag
    14701 aagctggata tgtgatcgtc agtggctgcg cgaaagggat agatacatac ggtcaccaag
    14761 cagcaatctc tcatggtgga cggacaatcg cagtcattgg aacagggata gagcaagtct
    14821 atcctgctga aaatcgtgac ttgcaagagg tgattgcaaa tgatcatctt cttttgagtg
    14881 aatatcctcc tcaatccggc ccgaaaaagc attgttttcc aatgagaaat cggattatcg
    14941 cagctttgtc taaaggaaca tgtgtctttg aagccaagaa aaaaagcggc tctctgataa
    15001 cagcacagca agcattggat cttggaagaa cagttttttc tgttccagga aatattttaa
    15061 cagaacattc taccggatgt catcatttga tccaagacgg tgcagtttgc acggtttcgg
    15121 gtgaagatat cttggcagaa tttaaagaat aacaccatac ttttcatttg cttttcttga
    15181 caaacgtttt ctgaatggat atgattagct acgatttgta tgaatcctct tttaatagta
    15241 ggaacacgac aatggaaagg agtccattca gtaatggcgt ataaatattt agtgattgta
    15301 gaatcacctg caaaagccaa gacaattgag aaatatcttg gacgaaacta taaagtaatg
    15361 gccagtgttg gccatataag agatttaccg aaaagtaaaa tgggtatcga ttttgaaaac
    15421 aattatgaac cccattatat ttctatacgc ggaaaaggcg atgtcatcaa aagcctgaaa
    15481 gccgcagcaa aaaaagctca aaaagtttac ttggcaagtg acccggatag agaaggagaa
    15541 gcgattgctt ggcatttagc gtacctactt gggttggatc tgaaagaaaa aaatcgggtg
    15601 gtcttcaatg aaatcacaaa agacgcagtc aaagcagctt ttaaggaacc aagaacgatc
    15661 gatgtagatt tagtagatgc acagcaagct cgtcgtacct tagacagaat cgttggttat
    15721 tcgatcagtc ctattctctg gcgtaaggtc aagaaagggt taagtgcagg acgtgtccaa
    15781 tctgtcgctt taaaaattat tattgaccgt gaaaaagaga tccgagaatt tgttccagaa
    15841 gaatattgga gcatcgacgg taattttaaa aaagctcgca agaaattcaa agcaaatttc
    15901 tggggaatcg acggtaagaa aaagaaatta ccagatgcac aaagtgtaaa agaagtcact
    15961 gctagaattg atggaacaga cttcgaagtt aaaaaagtag aaaagaaaga gcgcaaaaga
    16021 aaccctgcga atcctttcac tacaagtagc ctgcaacaag aagctgcccg taagttaaac
    16081 tttcgaacaa gaaaaaccat gatggtagca cagcaattat atgaaggaat cactttgggt
    16141 aaacaaggaa cagtcggctt gattacatac atgcgtactg attctacccg gattgccgat
    16201 acagcaaaag cagaggccgc agcatttatt gaagatacgt atggaaaaga attttctaga
    16261 catgatcacc gaaagatcaa aaatgctcaa ggcgcacaag atgcccatga agctgtgcgt
    16321 ccaacgagtg tgcttcgtac accagatgaa ctcaaacaat atttagataa agatcaattg
    16381 aaactttata cattgatctg gtcacgtttt gtggctagtc agatgacacc agctatttta
    16441 gatacaatga aagtgacatt agaacaaaat ggtgtcatct tcattgcgaa cggttcaaaa
    16501 atcaaattca aaggattcat gcaagtgtat gtggaaggta gagatgacgg caaagaagag
    16561 aaagaaaata ttcttccaga acttgaagaa ggcgatgtag tccaatctgt tgatattgaa
    16621 ccaaaacaac attttactca accaccagca cgttttagtg aggctacgtt gattcgtgca
    16681 ctagaagaaa acggggtagg gcgaccttcc acttatgcac caacacttga tacgatccaa
    16741 agacgctact atgtaaaatt gacacaaaaa agatttgagc cgactgaact aggagaaatt
    16801 gtcaattcat taatctgtga attcttccct caaatcgtcg atatccattt cacagcagaa
    16861 atggaaggag atttggataa aattgaagaa ggcacggaag cgtgggtcaa agtggttgat
    16921 cgtttctata aaccgtttga aaaagaactg acgaatgcgg aagaaaaaat cgaaaaaatt
    16981 caaatcaaag atgaaccagc aggattcgat tgtgatgtct gtggacatcc gatggtgata
    17041 aaattaggga aatatgggaa gttttatgct tgcagtaatt ttcctgattg tcggaatacg
    17101 aaaccaatcg taaaagaaat cggtgtgact tgcccagtat gtcatgaagg acaagtcatt
    17161 gaacgaaaat ctaagaaaaa cagactattc tatggttgtt cccgtttccc agaatgtgaa
    17221 ttcacttctt gggataaacc agtgggcaga aattgtccaa aatgtgacgg atatctggtc
    17281 gagaagaaag tcaaaggcgg taaacaagta gtctgtgtaa atggagatta cgaagaagac
    17341 atccaaaaat aatagaaaag aggttctttc attgaaaatg gtaaatgttg ttggagcagg
    17401 gcttgccgga agtgaagctg cttggcagat cgcacaagca ggagtccctg tccgtttata
    17461 tgaaatgcgt ccagtcaaat cgactgaggc gcatcacaca agtaattttg ctgaactagt
    17521 atgttccaat tcattaagag gaaacagttt ggctaatgca gtagggttat tgaaagaaga
    17581 aatgcgccga ttgaattcgg tagtaatcac ttcggcagat gaaacagcag tgccagctgg
    17641 tggtgcctta gctgttgacc gcgattcttt ttctgaaaca attacccgaa aagtcaaaga
    17701 gcatccatta gtaacagtga taaacgaaga aattactgaa ataccggaag gaatcaccgt
    17761 agtagcaaca ggacctttga cttctcatcc attagcagaa agcatcaaag cattcaatgg
    17821 ttcagatggt ttttatttct atgatgcagc tgcaccaatc gtagacaaag ctacgatcga
    17881 tatggataaa gtatatttga agtcccgcta tgacaaagga gaagcagctt acttgaactg
    17941 tccgatgaca gaagaggaat tcaaagcttt ccatgaggca ctaatcaatg ccgaagtggc
    18001 agaacttaaa tcttttgaga aagaaaaata ttttgaagga tgtatgccaa ttgaagtaat
    18061 ggcacagcga ggattcaaaa cgatgctttt cggacctatg aaaccagttg gattggaaga
    18121 tcctaaaact ggcaaacgtc cttatgctgt tatccagctg cgtcaagaca atgctgcagc
    18181 ttctttatac aacatcgtgg gctttcagac tcacttgaaa tggggcgagc aaaaacgtgt
    18241 tttccggatg attcctgggt tagaaaatgc tgaattcgtt cgctatggtg tgatgcaccg
    18301 aaacagcttt atgaattcgc cagaattgct gaaaccgact taccaatcaa cgaaacgcga
    18361 agatctgttc tttgcaggtc agatgactgg tgtagaaggc tatgttgaaa gtgcagcgag
    18421 cggactactt gctggaatca acgccgcacg cttagcaaaa gaaatggaac cagtcgagtt
    18481 tccacaagaa acagctattg gaagtatggc ttactacatt actcatgcgg aaggaaaaca
    18541 tttccaaccg atgaatgcca actatggtct gtttcctgaa ctgccagagc gcattcgaga
    18601 caaaaaagca agatatgaag caattgcaca acgagcttta aaagctaatg aggcaataaa
    18661 aacagaatta gaaaaaactg caatcccaaa tgagtaagga aactccatga agagggtgag
    18721 acggatagtc tcgctctttt t
//
LOCUS       DARBEC010000057        17975 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.058, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000057 DARBEC010000000
VERSION     DARBEC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.058"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(24..722)
                     /locus_tag="SCL80_001841"
     CDS             complement(24..722)
                     /locus_tag="SCL80_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEG1107702.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(715..2067)
                     /locus_tag="SCL80_001842"
     CDS             complement(715..2067)
                     /locus_tag="SCL80_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEG1107703.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(2290..3690)
                     /locus_tag="SCL80_001843"
     CDS             complement(2290..3690)
                     /locus_tag="SCL80_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEG1107704.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            3851..4588
                     /locus_tag="SCL80_001844"
     CDS             3851..4588
                     /locus_tag="SCL80_001844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEG1107705.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            4713..5192
                     /locus_tag="SCL80_001845"
     CDS             4713..5192
                     /locus_tag="SCL80_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEG1107706.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            5189..5956
                     /locus_tag="SCL80_001846"
     CDS             5189..5956
                     /locus_tag="SCL80_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107707.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            6186..6755
                     /gene="lepB"
                     /locus_tag="SCL80_001847"
     CDS             6186..6755
                     /gene="lepB"
                     /locus_tag="SCL80_001847"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEG1107708.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(6859..7461)
                     /locus_tag="SCL80_001848"
     CDS             complement(6859..7461)
                     /locus_tag="SCL80_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1107709.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            7567..8036
                     /locus_tag="SCL80_001849"
                     /pseudo
     CDS             7567..8036
                     /locus_tag="SCL80_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            8142..8726
                     /locus_tag="SCL80_001850"
     CDS             8142..8726
                     /locus_tag="SCL80_001850"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEG1107710.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            8729..9802
                     /gene="prfA"
                     /locus_tag="SCL80_001851"
     CDS             8729..9802
                     /gene="prfA"
                     /locus_tag="SCL80_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEG1107711.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            9795..10634
                     /gene="prmC"
                     /locus_tag="SCL80_001852"
     CDS             9795..10634
                     /gene="prmC"
                     /locus_tag="SCL80_001852"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEG1107712.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            10627..10806
                     /locus_tag="SCL80_001853"
     CDS             10627..10806
                     /locus_tag="SCL80_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107713.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            10828..11853
                     /locus_tag="SCL80_001854"
     CDS             10828..11853
                     /locus_tag="SCL80_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEG1107714.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            12028..13272
                     /locus_tag="SCL80_001855"
     CDS             12028..13272
                     /locus_tag="SCL80_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEG1107715.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            13425..14372
                     /locus_tag="SCL80_001856"
     CDS             13425..14372
                     /locus_tag="SCL80_001856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1107716.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            14506..15135
                     /gene="upp"
                     /locus_tag="SCL80_001857"
     CDS             14506..15135
                     /gene="upp"
                     /locus_tag="SCL80_001857"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEG1107717.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(15274..15516)
                     /locus_tag="SCL80_001858"
     CDS             complement(15274..15516)
                     /locus_tag="SCL80_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107718.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            15637..15709
                     /locus_tag="SCL80_001859"
     tRNA            15637..15709
                     /locus_tag="SCL80_001859"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:15670..15672,aa:Thr,seq:cgt)
     gene            complement(15828..16466)
                     /locus_tag="SCL80_001860"
     CDS             complement(15828..16466)
                     /locus_tag="SCL80_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1107719.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(16589..17221)
                     /locus_tag="SCL80_001861"
     CDS             complement(16589..17221)
                     /locus_tag="SCL80_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEG1107720.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            17572..17871
                     /locus_tag="SCL80_001862"
     CDS             17572..17871
                     /locus_tag="SCL80_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1107721.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
BASE COUNT         6157 a         3150 c         3553 g         5115 t
ORIGIN      
        1 tcacattccc ctttttcgaa taactaggct gtttgtcctt caagataggg cagatccaat
       61 tctttaccgt aacgttgttc taaagctagg cggatcaaac gagctgccag gttctcattt
      121 cttgcaagga taggtccatg gaaataagaa ccgaagacat ttcggtaaat gacaccttca
      181 gattggtctt caccattatt tccttgacct tgtaccactt tccccaaagg tcgttcacct
      241 tcacctaaga aagtccgacc attgtggttt tcaaatccat aataagtttc attgaattct
      301 tcgttatgga taacgatatc accgataaac cggttatttt cctgacttaa cgtgtagtga
      361 tccagtgcac tgattccttg gatcttttct ccattagctc ccatgtagta gtgacctagc
      421 aattggtagc caccacaaat ggctaacatg acgccatcat tttcgatata gttgatcaaa
      481 gaatctttct ttttctggat gtcttttgaa atgattaatt gctcaaagtc ttgaccaccg
      541 ccaaagaaaa ccaagtcata tttatcagca tcaaaaggtt catgtatact aacgatctcc
      601 gtatggcaag aaacccccat tttttctgct atatatttca acatcaaaag gtttccgtta
      661 tcaccatatg tgttcatcaa atttccataa aggtgggcga tattgagttc ataattagcc
      721 acggttcatt cctcctttga taaatccttg agcagctagt tctttacgaa gctgcaatac
      781 agcagtgtaa gtagctaaaa tatagacatg ctcagttggc aattctttga ttttttcgat
      841 tacttctggt aattcttttg tttctgtcaa tttattttct ggaatcccgg caacttttag
      901 acgtaaagtc atatctgtat gacggtctcc tccagaaata acagctggga tatccatatc
      961 ggcaaagttt tcaaaattac catcccaaat ccagctgaca tcgattccgt ctgcgtaatt
     1021 ggcatttaat aaagccacta aagaaaatgg ataaggagct agaccaatca tatcgattac
     1081 ttgattgata cctacaggat ttttgacaag tactaatgta cattttttcc cattgatatt
     1141 gatgacttct tgtcggccga atactttctc gtcatatcct aaaccttgtt tgattttatc
     1201 aggatcgaca ttgaagtact ctgccactgc agtagctgct aaagcattgt agacattata
     1261 cataccgcca acttcgattc cgtattcatg accatcgatc acgaaatctg ctgacacatt
     1321 gtccatccgt accatatcag ttaatcgata atccaattca ggacgtttga agtcacaatt
     1381 tggacagtaa tatttcccta agtttgcata agtgatcatt ttataatgca agatgtgatg
     1441 acaatttgga cataagacac cgtctgtatt gtaatgagcc atttgttcac gatcttcttc
     1501 atggtcaaag ccataatatt ttctaggatt gactgtttcg atagaattga agattggtga
     1561 atcaccgtta ctgataatcg ttgcatttgg tgactttgcg gcaccgtcta caatcatttt
     1621 ataggtcgta tagatttctc cataccgatc catttgatca cggaaaatat ttgtaaataa
     1681 gaacagttct ggtttcatat attctgtgac tttgctcaaa cttgcttcat ctatttctag
     1741 gacagcaaac tttttctgtc ctttttttgg ttttgcctgc aagaaagtcg atacaattcc
     1801 ctgtaccata ttcgcaccag tagggttggt tagaacttca tcgaattgct ggcgcaaaat
     1861 attgactgtt aaagcggtag tcaatgtttt accgtttgtt ccggtgacaa cgacgacttg
     1921 gtagtctttt gccaaactat ctagtatatg tggatcgact ttcagcgcta gtttacctgg
     1981 taaacttgat ccgcctttaa aaaaagtttt taatgcccat tgcgacgttt ttccaactgc
     2041 aatcgcaaaa tgactgcgta ttcccattat gtaacctcca aaaacgattt gctgacgaat
     2101 aaattcaact aatcatacca tatgttgaaa aaaaggttaa attgcttttc ttattcttaa
     2161 gaaaaggtta cggctgaaag tgcatgaaat cggacaaaat cgcatgattt aacaaaaaaa
     2221 cagcttgaaa caaaatgatt gtttcaagct gctgtgattt tattattccc tcttacctat
     2281 attcccattt taaccgataa taattttctc gcttggatat tcatagccta gatctcgtgt
     2341 ttcttttggc acgaataaca tcaatgtata tagcattccg atccgcccaa taaacatcag
     2401 tgtagcaatc gtgattttgc ccacaacaga caagtctccg gtaatcccta aagataaacc
     2461 agttgttcca aacgcagaag ttacttcgat gatgatcgag atcagcgttt gttcttctgt
     2521 agcagataaa aaaacgatac aaaagaaaca cataccaaga gaaagcataa agacaacgac
     2581 tgattttcgc acatcatctt gatcgattct ccgtccaaaa atattgatgt tatcttcact
     2641 tttgaggaag gaatatagat acaaaccgat gattgctaca gtagttgttc gaatcccgcc
     2701 gcctacagaa ctaggactgc atccgataaa catcagtaaa gaaaaaatga tcagtgttgt
     2761 gatttgaaaa tcacccaaat cgtgaatctg cagaccagca ttacgagtcg taatcgaata
     2821 aaacatcgaa ttgatccatt tgacactcat attggaatct tggaataaat ggtccttttc
     2881 caatagataa atcaagacag ttcccgaaac aaacaagata acaaaagcta acacagctaa
     2941 tttggtaaac aaggaaaaac gaaacggaag cttcgcattg gaacgtttgt ataatagcca
     3001 ttcccggcac tccatcaata caggaaaacc aattcctccg acaaaaatca aaaacatgat
     3061 caggatcaag aaaaaatagt catgtgcaaa gggcttgatg gaatccccag tgacgtcaaa
     3121 cccagagttc gtgacagccg atatcgcctg ataaaaaccg taaaaaacgg catcacgcca
     3181 acgatcaaaa tagcctctgt aataaaaata aatagaaaag aatgtcccaa aaagtaattg
     3241 aaaccagatt aaaatagcaa aagtgatccg aatcagtcgg acgataccag acaaacgcgg
     3301 ttgattcata tccgtcatga tcagctgtcg ctgtttcagt gtgatccgtc gtttagaaaa
     3361 aatgacaaaa gcagtagaaa tcatcatgat ccccaaacca ccaacttgaa ataatatttc
     3421 cagcagtata ataccgttat cattgaacac agaattgata tcaaaagtcg atagtccagt
     3481 cacactcact gtactgattg ccatgaacag catgtccaca aaagaaacat ggctatctgg
     3541 ttcgcgaaaa atcggcatat aaaatagaac gagtgataag accgtcatca atatataata
     3601 aaaaacgatg atttggatcg atgagaaatg attggcagca aacttctgtc ctcgccgctg
     3661 cagccttcga aagaataaat caacatgcac agtactatcc tcctgacaat agtcgtttct
     3721 accagtatac aaggggaatt aggaatactc aaagaaatta tcaaacagat aacaatacag
     3781 ctttcgatta aaatagaaat ggcttataat aaggaaaatc ctagaaaaaa gaaggaggag
     3841 gaataacaaa ttgattaatg ctcaacaaat cgtagaaaaa atgaaaaatc aaaaaataaa
     3901 ttatgataca gttctgaaaa aaatgatcat gcaatgggag aaagaagaag taaagcctaa
     3961 gatattaata catacatgct gtgctccttg cagtacgcac acattggaat tcatgtctca
     4021 atatgcagag atcacgttat ttttttcaaa ttcgaatatt catcctaaaa gtgagtattt
     4081 gcgaagatta catgaacaaa aacgatttgt agaccaattt aatgagaaaa ctgaccatca
     4141 tgttgcgcta atcgttgatg aataccgtcc aaatgaattc accaaaatgg ttttaacaaa
     4201 ccatcttgaa aacgaaaaag aaggtggtgc tcgctgcagt gcatgcttca atatgcgatt
     4261 agatcttgtt gcgcaaaaag cgcaagaact aggatacgat tactttggaa gtgccctgac
     4321 catctcgcca aagaaaaatg cagctttgat caatcagatc ggaatggata tccaaaagat
     4381 ttacgggaca caatacttgc caagtgattt caagaaaaac aaaggctatg aacgctcttt
     4441 gcaaatgtgc aaagagtatg atatttatcg ccaatgttat tgtggttgcg tgttcgctgc
     4501 caagcagcaa ggatgcgatc tgaaagaaat caatcgggaa gcaaaagctt acttaaaaac
     4561 atcaaccgta gtttttgagg aaaattaaag aaatctttcg gaaaaacgta agaaaaaaat
     4621 aaagaattta tggtagaaat aaggtagact gtataatttg tccgtagtaa agtaaggaaa
     4681 caagaataaa tttcaatgaa ggaagagata agatgcgaaa agtagcacct attttgaagc
     4741 caatcgctgt attaacaatc tcgacgattg tcgcgactac tacaggtatt gtgaatagtt
     4801 ccagctaccg tgcaccaagt tcaagttcta gtactgtaga agaaacaaca ccaactactt
     4861 cagtctcgac atctgaacca acaacaacgg aaagtttgaa tgaaaccact acgacaagta
     4921 catgggacga tacaacaact agttcgtcgt atgatgagac atatacaact gacagctcaa
     4981 gcgaaacaag taatacggta ccagatggca ctccatcgga ttcaaccaat ccaagtacaa
     5041 gtccggatac aagtgctagc tccgaaacga taccaagcag tacgactgta gatgataaca
     5101 cgcaaaatga tgatgcagca cgtacagcac agcaaaacca agattcagga aatacccaaa
     5161 cgtataacca gcaagcaggt gattctcaat gacattttta ctagacctta tcaacggggt
     5221 gcataaattc cttggctatt tagacatcag tcctaaatat cttaaccgtg gatatacgat
     5281 tttaagtgtg attcccacgc tttatttatt gagaattgtt tatggacttt ggcaaaacca
     5341 aaactatctt caatttttct tatatggact tgcatttcta gtgcttgttt attttactgt
     5401 tttaaatgta ttttattact ttttagataa aaacacgaaa gccgatgtca ctcagctttt
     5461 tgtgaagtat ttacctgatg aagcgttcaa catccagaca gaagaatcga tagataatgg
     5521 aaattcgatc gataaagtga atacacaaga ggtcatggtt tcttatgacg aagattatca
     5581 attgaaatta gccgagaata tgcggtattt gattggaaat ggtgagatca aaacaaatga
     5641 actcggtcgc atggatggat ttctggtcga tagaaataca ttgtatcctt attactatgt
     5701 caagaaaaca ggagagaaag agtatgttct aaatattgga cgaagctact cggacatgga
     5761 aaaagtaggg accattcgac ttgactcaac agaagacaca ttggaacctg tcggattgtt
     5821 catcgtcggc ggtgattttg tcaaagacgg gttacgctat catgagcctt atcgtctgaa
     5881 attgattgca aaaaaggcac agtcgcagga agaaccagtg gtttatagtc gtagccaaag
     5941 aagaagacat tcataatgtg atgaaacgcc gtgtatcagt ggttgcgata aaaacgttta
     6001 ttcggaataa gggggatgtg acacgtcttt tgtcacgttc cttttttata taacgagaaa
     6061 gataagacga acacatcact tattctttcc tttcatttat aaacattgta gaataaagaa
     6121 agtacaacga agtattgtga aaaaacgtaa taaataacga ggaaaataca agtaggaggt
     6181 aagctgtgaa agaatttttg aaaaattggg ggattttgat ccttgtatta gcggctattt
     6241 tattagctag agtgtacgtg tttacaccag ttactgtaaa cgggcattcg atggacccta
     6301 cattaagtga tggacaacga ttgatttcat ctaagatctc aaactatgaa cgaatggata
     6361 ttattactac aaaggaaccg ggggatgaag aacggatgat tgttaaacgg atcatcggta
     6421 tgcctggaga tacagtcaag atggaaaatg accagttgac cattaatggc aaaaagtatg
     6481 atgaacctta tctggatgag ttcaaaaaag aattttcaga agataagtta caaggagaat
     6541 atgcttatag ctcaggattc caagcacaag cagaaagttc atccaccttt acgagtgact
     6601 ttgaatatac tgtgccaaaa ggaaagtatc ttgttctagg cgataaccgc ttgatttcaa
     6661 aagacagccg aatgtttggg ttagtcgaca aagatatgat ccaaggaaaa gttgttttcc
     6721 gttattggcc tctttcagaa attaaaatca tgtgatgaaa aaataaaaag aagctgtgac
     6781 atgttttttg tcacagcttc tttttccgaa aattccttct tatttcttgg agttgctcac
     6841 tttttttgca atctcatttt aagctttttg gctgatggat aagaaaagcg aagcgttttt
     6901 gttcataaat agcttttccc cacgatggaa ggtatgacta gtagcagaga caaagcccgt
     6961 tttttccaat cgattattca attcttcttg gctaaagcca ttatggactt tcggatgact
     7021 aatttgttca ttcttgtcaa agtctacgat gatcaattga ccgccaggag ctaaaatttc
     7081 ataaagtttt gtaagaatat tctccgtatc aggaatgtgt aaaagtacaa gtgatagaag
     7141 gatgagatta gcttggacag caggaaattc gacagaagca tcagcgtgga tcgttcggac
     7201 atttttcaga tctgctgctt ggatcttttc ttctgccatt ttcagcatag tttctgaagc
     7261 atcggcaatg atcagttctt tgaatctttc cgcaagcggt aaactaacaa gcccagttcc
     7321 gcctccgtaa tccagcagga ctttttggtg gctgtcgcgc ggtagataag tagttagttc
     7381 ttcgcgaata atcttggcta atgcttgacg gtcaggggaa tcataatggt tagcgatgga
     7441 atcaaaaata ttttttgtca tcaaatagct ccttacgtaa aactttgaaa ttttaactta
     7501 ttatagcacg tgaaaagaaa gaaagcatgc tatactcaat tcaaaaaggg agaatctaga
     7561 aagaatatgc cgatatcgtt tgtaaaagat agagaggaaa aaggaaagtg tgtccgagaa
     7621 atattactag acttgcctga gtggtttggg cttcctgaat caacggaaaa atatatcgag
     7681 gaatcaagta aactaccttt atggtgtgaa aaaagaaaag aagaatattt aggctttatc
     7741 actctttcac agacaagtga agatactgcc gaaatctatt ctattgtatg ggaataaaaa
     7801 aagactatca tcaccaagga ttaggaacaa aactttttaa agaagcagaa ggttacgcag
     7861 caaaacatta caaatatttg caagtgaaga ctgtagacga ggggcactac tctatatatg
     7921 atcaaactat ttgtttctac gaatcattag gattttcaag gttggaagtt tttccaaatt
     7981 tatgggatga atggaaccca tgtttagttc ttgtcaaaaa attggaacaa aagtgagcaa
     8041 tagatttaat actaatctgt gcttgatgaa aattctcact gtaaaaaatg ctgaaaatca
     8101 agtataatac cataggaaca aactggaagg tgaaaaagag catggcacaa ctatttttta
     8161 agtatggcgc aatgaatagc ggcaagacga tcgagatcct aaaagtcgca cataattatg
     8221 aagaacaaaa taagccagtt gtcttaatga caagcggtat tgatacaaga gatggtttag
     8281 gcgttgtttc aagccgaatc ggactgaaac gtaatgcgat gccgatattt gaagaaacag
     8341 acgtatttga cgtaattcag aaaatggaaa gcaaacccta ttgtgtgctg atcgatgaag
     8401 cgcaattctt aaaaaagaaa catgtcattg cttttacgag aattgtggac gaattggata
     8461 ttccggttat ggcatttggt ttgaaaaatg attttcgaaa cgagttattt gaaggatcaa
     8521 aatatttatt gctttatgca gacaaaatcg aagaaatgaa aacaatctgt tggttctgcc
     8581 acaaaaaagc aatcatgaat ttacattata ttgatggaaa ccctgtttat gaaggcgatc
     8641 aagtgcaaat tggcggaaat gaagcttatt accccgtttg tcgaaaacat tactttcacc
     8701 catcaatgat tacagaagga gaataagcat gtacgatcaa ttacaagcaa ttgaagaccg
     8761 ttatgaagag cttggagaac ttttaagtga cccagaagtg atttctgata caaagcgttt
     8821 tatgcagctt tcaaaagaag aagcaaacac gcgcgaaaca gtcgaagttt atcgtcgtta
     8881 taaagaagta gtacaaggaa tcaccgatac agaagaactg cttggtgaaa aactggatga
     8941 tgaaatggct gaactagcaa aagaagaatt atctgaatta aaaaaagaaa aagaagtctt
     9001 agaagaaaaa atcaagatct tgcttttacc aaaagatcca aatgacgata aaaatatcat
     9061 catggaaatc cgtggagcag caggcggaga cgaagcagca ttattcgctg gcgatctttt
     9121 caatatgtac caaaagtatg ctgaagcaca aggctggaaa acagaagtga tggaagctaa
     9181 cgttaccgga atcggtggat ataaagaagt gatcatgatg atcaccggag acaacgtata
     9241 ttcgaaatta aaatatgaaa gtggtgcgca ccgcgtccag cgtgttcctt caactgaatc
     9301 gcaagggcgt atccatactt ccaccgcaac agttgtcgtg atgccagaag ctgaagaagt
     9361 agagatcgat attgaagaca aagatatccg tacagatatc tatcatgctt caggtgctgg
     9421 cggacagcac gtcaataaaa cagcatctgc tgtacgtttg actcacttac caacagggat
     9481 cgttgttgct atgcaagatg aacgttctca aatcaagaac cgtgaaaaag caatgaaaat
     9541 cttgcgtgct cgtgtgtacg ataagatcca gcaagaagca caaagcgagt atgatgccaa
     9601 tcgaaaatca gcagtcggaa ctggggatcg ttcagaacgg atccggacgt ataatttccc
     9661 gcaaaatcgt gtgactgatc atcgtatcgg attaactatc caaaaattag atcagatttt
     9721 agcagggaaa ttagatgaga tcgttgatgc attgatcatg tatgatcaaa catcaaaact
     9781 ggaagctatg caaaatgggt aaaatgacct atcgagaagt cctgtcacgg gcttcttctt
     9841 ttttagagga tcatgggaaa gaagcatata gtatccaatt tttatttttg gaacgaaaac
     9901 aatggaaaaa acttgattgg ctccttcata tgaacgaaga gatttcagaa gaagaacagc
     9961 ggttgatcga aaccgatctg cgacttctgc ttgcagatca tccacctcag tatttgctag
    10021 gatatgcaga tttttatgat catcggttaa aggtaactga agcaacactg atccctcgac
    10081 cagaaacaga ggagttagtc gaatggtgtt tagacgagac gcctggtgta ccgttagaag
    10141 tgatagatat tgggacaggg actggagcaa ttgctattag tttgaaagca gcaagaaaaa
    10201 attggcatat atctgctgtg gatctttcag aagaagcatt agaagtagcg aaagaaaatg
    10261 ctcagaaaga aggaacgaaa atcagttttt atcatggaga tacattagga ccagtaatgg
    10321 atcagcaatt tgatgtgatt atttctaatc cgccgtacat cagtcggaat gaatgggaac
    10381 tgatggatga aagtgtccgg tcattcgaac ctaaaatggc cctttttgct gagaacgatg
    10441 gattagctat ttatgaaaaa atagcagttg aagcaccaag cgtactaaaa tcaaatggaa
    10501 agattttttt agaaatcgga tttcgccaag gtgaagcagt aaaaaaaata tttcagcaag
    10561 catttccaga taaaaaagtg gcgataaaaa aggatctttt tggcaatgaa cgaatgatcc
    10621 aagtttatgg ttgaagttgt ggatgaaaaa ttttttattt gtttttttat tataaaaaaa
    10681 cacttatttg acaaggatgt aaacgacttt ttggtttatc aacaagttta tccactattg
    10741 acaaatagat tatccacaaa cagtggaaaa cttgaggata aactcagaca aaataagaat
    10801 gtatgaaaaa ggggttgtgg ataaactgtg gaaacgaaac gatatgatga aacagaattg
    10861 caagaggcag ccgaagcact gaaagaagga gaacttgttg cttttcctac cgaaacagta
    10921 tacggattgg gcgccaacgc tcttttacca aacgctgtca agaaagtgtt ttctgtaaaa
    10981 gggagaccgc aagataatcc attaatcgtc catgtcgcct cttttgaaca agtaaaagag
    11041 tatgtggaca actttcatcc ggcagcagag aagattgtga agaacttctg gcctggacca
    11101 ttaactttga tatttaagat caaaaaagac actttgccct cagttgtgac gggaggatta
    11161 tccacagctg catttcgtat accggataac aaaaaaacac tagaagtaat cgaactttcc
    11221 ggtgttccgc ttgtcggtcc aagtgcaaat acgtcgggaa aaccaagtcc aacgactgca
    11281 gatcatgtat atcatgatct acaaggaaaa attaccggga ttattgatga tggagctacg
    11341 cgaattggtg tagaatccac tgttcttgat ttaagtgatc caacagccat gcctatgatt
    11401 ttacgaccag gtgcagtaac gaaagaacaa atcgaagcag tcatcgaatc tcctgttgct
    11461 attgatcagc atcttgtaaa agaaaatgaa acaccaaaag cccctgggat gaaatacaaa
    11521 cactactcac ccgatacacg tgttctgatg gtcagagatg gcgattggtc aactgcagtt
    11581 caatgggcaa aaaataagaa gatccgtgca ggtgtcatag caagccccga aattgcggat
    11641 caagtgcgga cagatactgc agctgtttat atgtataacg acaactcagt agaagcggcg
    11701 gctaaaggat tgtttgcagg tctaagagga ttagatgaac caacacttgg attagatttg
    11761 atttttgtac aagtgtatcc agaaactgga ttggggaatg cttacatgaa tcgcttgaaa
    11821 aaagcagcag gacaaaatta ttttgaaaaa tgatcaattg gttattgtcg cattctttta
    11881 gtatgataca atgctatcaa gtaaatcttc tatcagtgaa cgaaaaacta agcgtaacta
    11941 atcgtaatag aagagatacg aatatttggc aatttgtttt tgatgggaaa aataaaattg
    12001 taggaaaata aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt
    12061 tgggctgcga tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct
    12121 gaaaactttg tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac
    12181 gcagaaggtt atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa
    12241 aatttagcga ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct
    12301 cactccggct ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg
    12361 atcttaggaa tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc
    12421 agtggcaaaa cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat
    12481 tataatgtgg tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc
    12541 agtgcttacg gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc
    12601 gcaaaactga tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct
    12661 aatccagttc cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct
    12721 cgaggcggca tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta
    12781 tttcctggta ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc
    12841 aaagaagcat tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa
    12901 gcaatggtca aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat
    12961 aatcatctga tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc
    13021 attttggaca gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt
    13081 ccattcaaaa caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa
    13141 gaagaagatg ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat
    13201 aacgctcaac tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg
    13261 cataaaaaat aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc
    13321 tgtttatttt ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga
    13381 ggtgtaattt tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct
    13441 cgttagaaaa ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg
    13501 aactagcacc ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca
    13561 aatattccgt cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata
    13621 ttcgtctcgt acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg
    13681 gagcaggctg ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg
    13741 ttcgtacgaa ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc
    13801 caacagcaat tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa
    13861 aaaatctgga tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta
    13921 cagtcatgac gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt
    13981 attatccagt cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact
    14041 attttttgac aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag
    14101 agcgtgctga aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc
    14161 cttcagcaat gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg
    14221 cgcgcaaact tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc
    14281 atgattttgt catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat
    14341 tatctacttc ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt
    14401 ttcaaaaacg gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg
    14461 ttttacaatt aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa
    14521 gtgatcgatc atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga
    14581 acaaaggttt ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca
    14641 cgggatatgc cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa
    14701 acgttgtctg gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt
    14761 gacggtatct tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat
    14821 gaagaaacat tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt
    14881 cagttgtttg tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat
    14941 gcattgaaag aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag
    15001 ggagtaaaag ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat
    15061 gaaaaattga atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg
    15121 tttggtacaa aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt
    15181 ttggtcattt ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa
    15241 aaaaccatcc caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt
    15301 aacgattact acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat
    15361 ctcaacaaca gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg
    15421 aattgtgaac attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat
    15481 agttgtatca aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta
    15541 taccacgttt tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa
    15601 tcagccaaat agagtatata atttcttttg gataaggcca gcttagctca gttggtagag
    15661 caacgcactc gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa
    15721 gacagatata tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat
    15781 aaagtatgct ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa
    15841 acgactagca atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc
    15901 gagttgcttt taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg
    15961 atgtgtgcta cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca
    16021 aagatgattg ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg
    16081 agaaaataca ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat
    16141 aacgggctga aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc
    16201 aaaaataccg agactgcggc actgattact acattgataa aaaaatattt ggctatattt
    16261 ttgacaggag cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt
    16321 gtaaatgcta taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat
    16381 agcaacccta acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct
    16441 attttttttc ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata
    16501 gagtcagtat acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct
    16561 aaaaatttat cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag
    16621 caaaaccaag tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt
    16681 caatcatttc aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc
    16741 tagcattttc taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat
    16801 ctgcgcttga taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat
    16861 ttacatagtc cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt
    16921 tagtacctgt taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata
    16981 tacagcctga aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat
    17041 cattggaagc aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta
    17101 tttggttttc gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt
    17161 catagatatg aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca
    17221 tatttaatta cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta
    17281 gcagaaaatg agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa
    17341 cgttcttaga attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa
    17401 taaaaatttt tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag
    17461 tgtattgaaa ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac
    17521 tttgtttata gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa
    17581 agtgaagaaa tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc
    17641 aaagaaaagg aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat
    17701 tatcctaaag tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa
    17761 ttgtctaaac cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa
    17821 gaattaggtc gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt
    17881 attcatctca atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat
    17941 ttgaacagag tctgtatttt tttattctaa ataaa
//
LOCUS       DARBEC010000058        16916 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.059, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000058 DARBEC010000000
VERSION     DARBEC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16916)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16916)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16916
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.059"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..258)
                     /locus_tag="SCL80_001863"
     CDS             complement(<1..258)
                     /locus_tag="SCL80_001863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107722.1"
                     /translation="MERQESIREVQEVPTEQAKETQEGRSFSYGKNFSFLRPSIQISV
                     FVLWRIHNNRYSVGSQLFYQEICHALSKKYSQNAYKEALAFL"
     gene            complement(274..924)
                     /locus_tag="SCL80_001864"
     CDS             complement(274..924)
                     /locus_tag="SCL80_001864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107723.1"
                     /translation="MKKRFIYVCVGGLFLFGVGSTFFLIHEKLQLTAKEEKLTVEINK
                     FQEEKKGQEDLVTSLKEQLQQVESEVTTLKKANEELKNNDSTEKGNIQTFQKTVTEAF
                     EVLFNYEPDSFQERKNNCAPYLSDKLMKQYFKEGVSYGDSNGVSSELEKCSVYLKSTQ
                     KATIQGLVVVSYRNKIGDSSWNNAMNIFDVTFDPASKKITFMQNLGSSFNQQLLEE"
     gene            complement(935..2107)
                     /locus_tag="SCL80_001865"
     CDS             complement(935..2107)
                     /locus_tag="SCL80_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAP domain-containing protein"
                     /protein_id="HEG1107724.1"
                     /translation="MKKKLLLWCGFGCLPTLLLCMFGFLFMILLISSGSEEEEIDCSP
                     TEIVASTISLTNQGMETNGLQIAKEVQKHIPNTTLEGLAGMLGNFQQESSMSPTAIER
                     PNDPLSGHGIAQWTAGRTTALKNFAKERNKEWSDLGVQVEFLIHELNTTEKASQVALQ
                     AKSVEEATEQWQVKFERAGIPMMGNRITFANQWYAKLGKNDSDPVASITLENGANAGS
                     KDLTDYCLETTGTSSSSDSNVLNVAKSLIGYFHYSQPQRAQFGSVENPDKNGYADCSS
                     FVWLVLTKAGYKTPANVGWFTGSMTSDARGSKQYLQEIPESEAKAGDIIIVNLGSGVG
                     NDGHTAVLAEDWHGLSTKVIEQGGMNTNGVGEGQVNLSFGYLLNGGDICFARPIKK"
     gene            complement(2126..2998)
                     /locus_tag="SCL80_001866"
     CDS             complement(2126..2998)
                     /locus_tag="SCL80_001866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1107725.1"
                     /translation="MKKMILGSLFSATVLLGMVGTSAYADTTSPGDPSVPPTEIIPPT
                     EETPEVPEEPVLPPTDGGEETPEIPEIPETPETPEIPPTEENPDTGGGTETPEVPENP
                     DTGGGEGTPEVPPVEENPGNGGNTETPEKPSNPGGNEGNTTPPTTPPTNNGGTNTTPP
                     VQDVVVKPDGSIGSQQTGGQNVQIETNNASELTHIPTPSTPIETASGEKIVSVVDGVA
                     YKESGNGLTPISDKVEQLPSGNIAVKGSDGKMKVLPKTGEEVSLVATALGGLLASGSG
                     FAFWRKRKATQKMN"
     gene            complement(3020..3940)
                     /locus_tag="SCL80_001867"
     CDS             complement(3020..3940)
                     /locus_tag="SCL80_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107726.1"
                     /translation="MMMIRFTEVDNMSLLTDKKIALDKAFIQLKRENEELLARAEIGW
                     CTLEIYTENHEIPVFEGSYDFPVEEFHLISLIEQSLTENGQLSEEAQTFIQTLNSELP
                     KFYQVKKDQKNERRPTKIKKEKKTPLSVETAFTQSNGSLQKKKMSFQWLLFGFIVVVS
                     CGLFIMQLSTQREIEQLTKKEHQLQERIAFAEELATKQPQIDTFSRYFITNYYSGEMA
                     EERYRKKLQSFLTEKVAKQISPQKEKVKSILSWEMKKEGKHWQIAYVITLETEKKTQE
                     TKKVSFQLEEQEKNYLVSTLPELDDFEINQ"
     gene            complement(3940..4209)
                     /locus_tag="SCL80_001868"
     CDS             complement(3940..4209)
                     /locus_tag="SCL80_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107727.1"
                     /translation="MIIVTGIVLLGIGVLFGYHFAYFPKIGEWQQRQAIQKYFQVKGN
                     EYTYYAEDEDSYIVSLNNQEYRVKFSLNNPIQVVYAVEVVEEVFD"
     gene            complement(4206..4586)
                     /locus_tag="SCL80_001869"
     CDS             complement(4206..4586)
                     /locus_tag="SCL80_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HEG1107728.1"
                     /translation="MITNYKKFWQNSFNYKGKATRKEYWLVALMNALIGFGLGLAIGL
                     AGALNASETIIGCLIGVLIVYCFVVLVPSLSLSVRRLRDAGFHWAFIFFYFVPTIGSL
                     VLLVLYCLPTKESIEDKQVQEETR"
     gene            complement(5053..6516)
                     /locus_tag="SCL80_001870"
     CDS             complement(5053..6516)
                     /locus_tag="SCL80_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1107729.1"
                     /translation="MKKTSKCLSALLLVGQVFGYTPAVLANEQGNNQLTNSQSATITE
                     ETTTNSTGEENQPETPTEDTIDTPDNPEMNAPPTGSEEQPETPNQPDKPEDNQQPEEE
                     PSEEPNEQPSDESSQTPQKPSTETEAEPPTKTERPQKAPEQSVTVPMNDVPQAQSIGS
                     EETKNYHQAPSAPIQELVPSSTQDNEGTIHFEKDASVETFIRKIGESARKIGQENDLY
                     ASVMIAQAILESASGQSQLAQAPNYNLFGIKGTHNGKGVSFATQEDLGNGTLYTTQAT
                     FRQYESYEESLKDYAKLLKEGLVGNTSFYDGVWKSKAKTYQEATKFLTGRYATDTSYD
                     KKLNGLIETYDLIKYDKEVAGPQLSKEGYMVPLKNYSISSSFGWRGEEFHRGLDMAAN
                     QGEPIYASKAGTVIEASYHTLSGNYVTIEHEDGSVALYAHQQEYCVNVGQTVEQGQII
                     GYVGSTGNSTGPHLHLELALDGSLTKDKLINPETVLF"
     gene            complement(6580..9597)
                     /locus_tag="SCL80_001871"
     CDS             complement(6580..9597)
                     /locus_tag="SCL80_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1107730.1"
                     /translation="MFKRMATWLAIFVTFLNPMLGSVTAFAETINESNSTEEVMKTEE
                     SKSSEEAISTETTSETTEETTTQDTTEDVSPPNVMTQEVPEGYEKYFITIEDIQKATS
                     FSEQERLAQESEEWQAILEKNAQTRATNLTLTENGRKDVNMTRITPEGTMYTHDYMVR
                     WQIDGEDVFCIQEGAITSAGIQYDVTPLENLITTSAIRKKISLIGYFGYYSSGSMDNY
                     VLAQTYIWEVLGGSFVNYGTIGQSNYEAFKQTVDRKINNLLEDPSFDSQTVTVKVGEE
                     VEVKDTKGVFSQWDGSVVENTANVQVRKSGNSLFIKANQNSNDGRISVSSLDPNSRGV
                     GSSFAYVDPVAQNLGKLYVPDPNRAGLTIKVLKNGDFKLTKLDAVTGKKVPNVTYEIK
                     RHDTGETKQYTTDAKGEIALKEIPAFYEKDGKYVQNKLDVKEVKAPAGYILDKQTHTI
                     TIEPNKEVTLTAKNDLQQFIPEGKKVKEVYNAEESWNQGKPVYDTVPAENIEFDIIAK
                     TDITAPDKETVLVKKGEVLDSVKTDKDGHWKANKKFYNGDQNKYQLIEKNVPDNYRQP
                     SEEQTTFSVAYGDNTTPVLTFELDVIDNLLKTGEVDFNKVDSINHLQLPGAKFLIEGM
                     TAHNKNVSFVYETKEEPEKLKLPDGTYKFTEIVFPEHYGQASGQTETILVTVKDGEVQ
                     PITVKNEEIVVPEEDQPEISTLFVTTTGGKDFNPKKDQELVDKVSQVFPKSAVGTEKY
                     WVTQFHKITADGKVTVLDTVKETRKVTDEKETFDVTFNYKADMVSDGDTIVATHIVYN
                     DKEEEEEFARHFDLTNKDQTVTAKEPKIETRFITVNGDQTFDPDKDQELIDHVSQEFP
                     ESTVGETKYWVTQFHKIDKNGKDTVVGTVETARKVSKASEEFKVSFDYKAGTLKDGEK
                     LVATHIVYKDKDHKEEEARHFDLKNEKQTLTAKGIKKETPTTPVKQTPTSTPKQSLPK
                     TGETVNDVLIALGLFLFVGVGAVLVRRKMKQSN"
     gene            complement(9748..10587)
                     /locus_tag="SCL80_001872"
     CDS             complement(9748..10587)
                     /locus_tag="SCL80_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1107731.1"
                     /translation="MKMLKVLALGIVVGGVSLAVGTNAEAAENKVWTPRSAEQIKAEI
                     KGNEYTIVWGDTLSAISQATNITVQKLADMNKITNVDLIYAGNKLVFNGNVASVQNSA
                     GETVAQTVIQEKDKVDPNQPVGQGTTSNTQTTPNTTTPTTPAGDQTGTTGGTTTPSTP
                     ENNGGTTQTPTQPGNGGNTGGETTPSNPSTGGGTTTPEEPSNPGGGDQGGNGGTTTPE
                     EPTIVVGEIGNSGKVFNTVDEAYDWANAEVSDPNSQWYNKGFMVDVIIYSDGSQKHTV
                     DFY"
     gene            10732..10977
                     /locus_tag="SCL80_001873"
     CDS             10732..10977
                     /locus_tag="SCL80_001873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107732.1"
                     /translation="MQLNNLNEIHDWAQNNLLNRKQAAKITGQNYDAFSSSLRRNNIK
                     PFLEIGDEPGPSIIRLYLREDIEKYAKQLEEKRQKRQ"
     gene            11040..11462
                     /locus_tag="SCL80_001874"
     CDS             11040..11462
                     /locus_tag="SCL80_001874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014862359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107733.1"
                     /translation="MNKKYIVSVGSIVLIVLAIIAFSVSNKTETQPQNTVQSTTTSQS
                     SDHSVYPSLNSLKETHGQDLIDLGSSQITTVLFEKKNIAFITPKEGTDLEFSIKVTNT
                     KENLFSIPDIQYTPNKLKIGDTFGLAKVNEQYFAYSLK"
     gene            complement(11556..11873)
                     /locus_tag="SCL80_001875"
     CDS             complement(11556..11873)
                     /locus_tag="SCL80_001875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107734.1"
                     /translation="MPVKGTSNFDFEIFYVDDEICGVFNKQKYTKEQAIEAWKNELFD
                     PEEEISFVVDESFVGWYHGNAEEEEVHYAGWWLEEGEPREQSVPVWVISENPLEEIEK
                     LEK"
     gene            complement(11900..12106)
                     /locus_tag="SCL80_001876"
     CDS             complement(11900..12106)
                     /locus_tag="SCL80_001876"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107735.1"
                     /translation="MNWLKNEAELYVFHEEMQEKHLVPNVRNYLFWLEEKNDAQVFGK
                     SAQSPKIKLQLTSKQWNEHEWYIY"
     gene            complement(12087..12269)
                     /locus_tag="SCL80_001877"
     CDS             complement(12087..12269)
                     /locus_tag="SCL80_001877"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107736.1"
                     /translation="MTKCERVSVTISFIALLLSVISLLNTAIFIGQVEKQKEISLSEW
                     RYEEVNEGMNNELVEK"
     gene            complement(12283..12633)
                     /locus_tag="SCL80_001878"
     CDS             complement(12283..12633)
                     /locus_tag="SCL80_001878"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107737.1"
                     /translation="MKKSYKVFENNEIKEQEAIILSAQTPEEALEQALPFLVESQNIV
                     VEELPEFDSKIVFSFNCKKESEISVSPEFYCRSDISVEGLVMCASAFIEIFANHLQIS
                     VKELLDLLQEITNE"
     gene            complement(12659..12961)
                     /locus_tag="SCL80_001879"
     CDS             complement(12659..12961)
                     /locus_tag="SCL80_001879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107738.1"
                     /translation="MLSKQEKKYIRQKMYAMIGNRAITELSSEELLQVQQLAQIIGSN
                     SIFDSNPLPEMTLETLTAKRYKELRAIGYRMIDIRRALRISDTKFREWRTSNGLAN"
     gene            complement(12964..13197)
                     /locus_tag="SCL80_001880"
     CDS             complement(12964..13197)
                     /locus_tag="SCL80_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107739.1"
                     /translation="MTISVGNKVKYIGTAIPRYTNKLVVVKNILVNGLILEYPKEDRR
                     KVVVEGNGTWNMNSFICGFTEVEVTNDNENRSG"
     gene            complement(14183..14505)
                     /locus_tag="SCL80_001881"
                     /pseudo
     CDS             complement(14183..14505)
                     /locus_tag="SCL80_001881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347542.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14628..15743)
                     /locus_tag="SCL80_001882"
     CDS             complement(14628..15743)
                     /locus_tag="SCL80_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1107740.1"
                     /translation="MATKRFTATRKYNELYYQFPKVFITSEKYRSLSDKAKIAYMVFR
                     SRLDLAIQRNQVDSQGFVYFEFTEQELANALNCCRRSVNTIKKELKEYELLEIEDMGF
                     DQQTGKRNKARLYLGELDTTENDVYSLGPVDSTSVDSAQGKEEEESVDNLAIFDDFDE
                     KNPCENSAQLFNNSSNNLDTENLDTKIDTTSPVEMADDWQKEFIAHYSESTFVPKDVL
                     TWIVRSSAEWREAKEQLNMIYDSKRIIEKRQRKYNQTHGLSDGSEYRILGETFAEDLS
                     KTYQRCIQNEKIARHEGKAIQNRKGFYFRALLNFWECCVRELNLDPMATKLSDQAEYS
                     LLQAKRSCSYEDLETQIYGLSQLPKISMHNWLEELDT"
     gene            complement(15749..16147)
                     /locus_tag="SCL80_001883"
     CDS             complement(15749..16147)
                     /locus_tag="SCL80_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1107741.1"
                     /translation="MVKNKFRATDVLTQATFHMPKLFFIKSSKYYAMTAQAKYAYSLL
                     NEYLEESLIEKRIDEKKHVYISYTNQELAILLNVSEEEIEEIKNELEDYDLLTIEFGR
                     IYLKELIATMEDVRVYQHQNSFLIYGQDEV"
BASE COUNT         4519 a         3512 c         2639 g         6246 t
ORIGIN      
        1 caaaaaggca agtgcttctt tgtaggcatt ttgactgtat ttttttgata aagcatgaca
       61 aatttcttga taaaatagtt gactgcctac cgaataccga ttattgtgta tccgccacaa
      121 aacgaacaca ctgatttgaa tcgacgggcg taaaaaagaa aaattctttc cataagaaaa
      181 agaacgtcct tcttgggttt cttttgcttg ttctgttggt acttcttgta cctctcttat
      241 tgattcttgt ctctccataa ccttctcctt ttcttattct tctaaaagct gttgattaaa
      301 gctacttccc aaattttgca taaacgtgat tttctttgac gctggatcaa aggttacgtc
      361 aaaaatattc atggcattat tccaggaact atcaccaatt ttattgcgat acgacaccac
      421 aacgagccct tggatagtcg ctttttgtgt gctttttaaa taaacagaac atttctctaa
      481 ttcagaagac accccgtttg aatcaccata ggaaacgcct tctttgaaat attgcttcat
      541 cagtttgtca gaaagatatg gtgcacaatt gttttttcgt tcttgaaaag aatcaggctc
      601 ataattaaac agaacctcga acgcttctgt cactgttttt tgaaaggttt gaatgtttcc
      661 tttttctgtt gaatcattgt ttttcaattc ttcatttgct ttttttaacg tcgtgacctc
      721 gctttctact tgttgaagtt gttctttgag tgacgttaca aggtcttcct gtcctttctt
      781 ctcttcttgg aacttgttta tctccaccgt cagtttctct tcctttgctg ttagctgtag
      841 cttttcatga ataagaaaaa atgtactgcc tacaccaaat aagaacaatc cgcctacaca
      901 aacataaata aatcgtttct tcatttgaca cctcttattt ctttatcggt cgagcaaaac
      961 aaatatcacc accattaagc aaatacccaa aggataaatt gacttgccct tcacctacac
     1021 cgttagtatt cattccccct tgttcgatta ctttcgtaga aagtccatgc caatcttccg
     1081 ctaaaacagc tgtgtgtcca tcatttccca caccagaacc taagttcaca atgataatat
     1141 cgccagcttt tgcctcgctc tcaggtattt cttgtaagta ttgcttactt cctcttgcgt
     1201 cgctcgtcat tgagccggta aaccacccaa cgttcgcagg cgttttatac cctgctttcg
     1261 ttaaaacaag ccacacaaag ctcgaacaat cggcatagcc atttttatct ggattttcga
     1321 cactaccaaa ttgtgcccgt tgcggttgtg aataatgaaa ataaccaatc agactttttg
     1381 ctacgtttaa tacattacta tcggaagaac tgcttgttcc tgttgtttct agacaataat
     1441 cggtcaagtc tttacttcct gcgtttgccc cattttctaa tgtaattgaa gcgacgggat
     1501 cactgtcatt ctttcctagt tttgcgtacc attgattcgc aaaagtaata cgattcccca
     1561 tcattggtat ccctgcacgc tcaaatttca cttgccactg ttctgttgct tcctctacgc
     1621 tttttgcttg taatgcaact tgtgaagctt tttctgttgt gtttagttca tgaatcaaaa
     1681 actcaacttg tacacctaaa tctgaccact ctttgtttcg ctctttggca aaatttttca
     1741 aagcagtcgt tcgccctgcc gtccattgtg caatcccatg cccagataat ggatcgtttg
     1801 gtcgttcaat cgctgtggga ctcatactcg actcttgttg aaaattgcct aacatacccg
     1861 ctagaccctc taatgtcgta tttggaatat gtttttgtac ttcctttgca atttgaagcc
     1921 cgtttgtctc catgccttgg ttcgttaatg agatagtact tgcgacaatt tcggttggac
     1981 tacagtcgat ttcttcctct tcagaaccac tactaatcaa aagtatcata aacagaaaac
     2041 caaacataca tagtaatagt gtgggtagac aaccgaaccc acaccataaa agaagttttt
     2101 tcttcatgtt tgatccttca ttcgcttagt tcattttttg tgttgctttt cttttgcgcc
     2161 aaaaagcaaa accgcttcct gaagccaata atccaccaag tgctgtcgca acgagagaca
     2221 cttcttcgcc agtcttaggc aatactttca tttttccatc agaccctttc accgcaatat
     2281 ttccgcttgg aagttgttcg accttgtctg aaatgggcgt caagccattt cctgattctt
     2341 tataagcgac accatcaacg acggagacaa ttttttctcc tgaagctgtt tcaattggtg
     2401 tacttggtgt tggaatatgg gttaattcag acgcattatt ggtttcaatt tgaacatttt
     2461 gtccacctgt ttgttggctg ccaatacttc catctggttt tactacgacg tcctgtactg
     2521 gaggcgtagt gttcgtcccc ccattattgg taggtggtgt cgttggagga gtcgtatttc
     2581 cttcattgcc tcctggattc gacggctttt ctggtgtctc tgtattacca ccatttcctg
     2641 ggttttcctc aacgggtgga acttcaggtg ttccttcccc tccgccagta tctggatttt
     2701 ctggtacttc aggcgtttct gtgcctccac ctgtatctgg attttcttcc gttggtggaa
     2761 tctctggggt ttcaggagtt tctggtattt cgggaatttc tggggtttct tctcctccat
     2821 ctgttggtgg taaaacaggt tcttcaggaa cttcaggtgt ttcttccgtt ggtggaataa
     2881 tttctgtcgg tggtacactt ggatcacctg gtgacgtagt atctgcatac gcactggttc
     2941 ctaccatacc tagcaataca gttgcactaa ataaacttcc taaaatcatt tttttcatgt
     3001 gtttttcctc ttctcttatt tattgattta tttcaaaatc atctagttcg ggaagcgtgg
     3061 aaactagata atttttttcc tgttcttcca attgaaaaga cacttttttt gtttcttgtg
     3121 tctttttctc ggtttccaat gtaatgacat acgcaatttg ccaatgcttg ccttctttct
     3181 tcatttccca ggataaaata cttttgactt tttccttttg aggactgatt tgtttcgcaa
     3241 ctttttccgt tagaaacgat tgaagctttt tcctataccg ttcttctgcc atttcacccg
     3301 aataataatt cgtaataaaa taacgactaa atgtatcaat ttgtggttgt tttgtcgcaa
     3361 gttcttccgc aaatgcaatg cgttcttgta actggtgctc ctttttcgtt agttgttcaa
     3421 tctctctttg cgttgaaagt tgcataataa aaagaccaca agatacaacg acgataaatc
     3481 caaacaaaag ccattgaaaa gacatttttt tcttctgaag agagccgttg ctttgtgtaa
     3541 acgctgtttc tacagaaagt ggtgttttct tttccttttt gatttttgtt ggtcgacgtt
     3601 cgttcttttg gtctttcttt acttgataaa acttcggtaa ttcggaattt aaggtttgaa
     3661 taaaggtttg tgcttcttca cttaattgcc cattttctgt taaagattgt tcaatcaatg
     3721 aaatcaaatg aaactcttct actggaaaat catacgagcc ttcaaatact ggaatttcat
     3781 ggttttctgt gtagatttcc aaggtacacc agccaatttc cgctcgtgcc aatagctctt
     3841 cattttctct tttcaattga ataaatgcct tatctaatgc aattttttta tctgttagta
     3901 aagacatgtt atcaacttct gtaaatcgaa tcatcatcat tagtcaaaca cttcttccac
     3961 cacttctacc gcatacacca cttgaatagg gttatttaaa gaaaatttca cccgatattc
     4021 ttgattgttc aaactgacga tatagctatc ctcatcttca gcataatagg tatattcgtt
     4081 gcctttgact tgaaaatatt tttggatcgc ttgtctttgt tgccattcgc caatctttgg
     4141 aaaataggcg aaatgatagc caaataaaac gcctatccct aataaaacaa ttcctgttac
     4201 tataatcatc tggtttcctc ctgtacctgt ttatcttcta ttgactcttt tgtcggtaag
     4261 caataaagaa ccagtaacac aagagagcca atcgttggga caaaatagaa aaaaataaat
     4321 gcccaatgaa aacctgcgtc tcgtaaacgt cgaacgctga gagaaagaga cgggacaaga
     4381 actacgaaac aatacacaat caatacgcct atcaaacaac caattattgt ttcagaagca
     4441 ttcaacgctc ctgctagtcc aatcgctaag cctaggccaa acccaatgag ggcattcatt
     4501 aacgcaacga gccaatactc ttttcttgtg gctttccctt tgtaattaaa gctattctgc
     4561 caaaattttt tataatttgt gatcatatct ttgtcctttc taatgtttat ttttgattct
     4621 agtttcttct gttttactaa caatgaaact tgccataaaa aaggtggcca atacaccacc
     4681 agctagaaat ccacttacaa ataacaaaat tttttcctcc tttctaaaaa atttcgccat
     4741 aaaaaggcaa agtatgaaca cgataaatac catgtacata ctttgccttt ttatgcaata
     4801 tatgttaact ctccaagcaa ttactgattt ttaaattctt tataaataaa ataaaaatcg
     4861 tttgagtaaa gaaataagtc aatgaagacc caaacgataa aaaatattcc taatactata
     4921 tttaataaac tacgttctag atagatacga gatttcaaat taaaaaacga aaatgagaga
     4981 tacaaatata tcaaatattt aaaaagtaac cacattttct ataccactcc ttatcagaaa
     5041 cagccttatt ctttagaata agactgtttc tgggttgatt aatttgtctt ttgttaaact
     5101 accatctaag gcaagttcta aatgtaaatg tggtcctgta ctatttcctg ttgaacccac
     5161 ataaccaata atttgtcctt gttcaactgt ttgtcctacg ttcacgcaat attcctgttg
     5221 gtgcgcataa agagccacac ttccgtcttc atgctcaatt gtgacataat ttcctgacaa
     5281 agtatgatac gaagcttcta tgaccgttcc tgccttgctc gcatagattg gttctccttg
     5341 attggctgcc atgtctaatc ctctatgaaa ttcttctcct cgccaaccga aggaactaga
     5401 aattgaataa ttttttagag gtaccatata gccttctttg cttagttgtg gaccagctac
     5461 ttctttatca tatttgatta agtcataggt ttcgattaac ccatttaatt ttttatcgta
     5521 gctagtgtct gttgcgtatc ttcctgttaa aaatttagtc gcttcctgat aggtttttgc
     5581 cttacttttc caaacaccat cataaaaaga ggtatttcca actaagcctt cttttaacag
     5641 tttggcatag tcttttaagc tttcttcata gctctcatat tgccgaaaag ttgcttgggt
     5701 cgtataaagt gtcccattgc ctaaatcttc ttgtgtcgca aaagagacgc cctttccgtt
     5761 atgtgtgcct ttgattccaa atagattgta gtttggtgcc tgagccaatt gactttgtcc
     5821 gcttgcggat tctaaaatgg cttgcgcgat cataacagaa gcgtataaat cattttcttg
     5881 tccaatttta cgagcagatt caccgatttt acggataaac gtttctacag acgcgtcttt
     5941 ttcaaaatga atagtccctt cgttatcttg tgtgctggaa gggactaatt cttgtatcgg
     6001 tgcgcttggt gcttggtggt agttttttgt ctcttctgaa ccgattgatt gtgcctgcgg
     6061 tacgtcattc ataggtacag tcacactctg ttcaggtgcc ttttgtgggc gttccgtttt
     6121 tgttggcggt tccgcttctg tttcggtact tggtttttga ggagtctgtg agctttcatc
     6181 agaaggttgc tcatttggtt cttctgacgg ttcctcctca ggttgttgat tgtcttctgg
     6241 tttgtctggt tgattcggtg tttctggttg ttcttcagaa cctgttggtg gagcgttcat
     6301 ttctggatta tctggtgtat ctattgtatc ttcagtcggt gtttctggtt gattttcttc
     6361 tccagtggaa tttgttgtcg tttcttcagt gatcgttgca gattgcgaat ttgtcagttg
     6421 attgttgcct tgttcattag ccaaaaccgc tggcgtatac ccaaatactt gacctactaa
     6481 taacaaagca gataaacatt tactcgtttt tttcacaaga ttgcctccat tcttcaatca
     6541 atcatctaga gacagctgat tgctgtctct agtcatactt taattgcttt gtttcatttt
     6601 acgacgaacc agaacagccc ccacaccaac aaaaagaaac agtcccaatg caattaacac
     6661 atcgttcact gtttctcctg tttttggtaa gctttgtttt ggtgtgctcg tcggcgtttg
     6721 tttgacaggg gttgtcggcg tttctttttt aatgcctttt gctgtcaatg tttgtttttc
     6781 attcttcaaa tcaaagtgac gtgcttcttc ctctttatgg tctttatcct tatagacaat
     6841 atgagttgcg accaatttct ccccgtcttt taacgtgcct gctttgtagt caaaggatac
     6901 tttaaactct tcagacgctt tagaaacttt acgtgcagtt tctaccgtac caacgactgt
     6961 atcttttcca tttttatcaa tcttatggaa ttgagtcacc cagtatttgg tttcccctac
     7021 cgtagattct ggaaattctt gcgatacatg atcgatcaac tcttggtctt tgtctggatc
     7081 aaatgtttgg tcgccattca ctgtaataaa gcgtgtttcg atttttggtt ctttcgcagt
     7141 cactgtttga tctttattgg ttaaatcaaa atgacgagcg aattcttctt cctcttcttt
     7201 gtcgttatag acaatgtgcg ttgcgacaat cgtgtcaccg tctgaaacca tgtctgcttt
     7261 gtagttgaac gtcacatcaa atgtttcttt ttcatcggtc acttttcgtg tttcttttac
     7321 cgtatctaat acagtgactt ttccatcggc agtaatttta tggaattgcg tcacccaata
     7381 tttctctgtc ccaaccgcag atttagggaa tacttgggac actttatcca ctaattcttg
     7441 gtcttttttc gggttgaaat cttttccacc tgtcgttgtc acaaacaaag tagaaatttc
     7501 tggttggtct tcttctggaa ccacgatttc ttcgtttttc acagtaattg gttgtacttc
     7561 tccgtctttg accgttacta aaatcgtttc tgtttgtcct gacgcttgcc cataatgctc
     7621 agggaacaca atttccgtaa atttatacgt accgtctggt aattttagtt tttctggttc
     7681 ttcttttgtt tcatacacaa aggacacatt tttattatgt gcagtcatac cttcaatcag
     7741 gaatttcgct cctggtaatt gtagatgatt gattgaatcg actttattga agtctacttc
     7801 acccgttttt aacaagttgt caatcacatc taattcaaac gtaagcacag gcgtcgtgtt
     7861 atcgccatag gccacagaga acgtggtttg ttcttcagaa ggttgacggt agttgtctgg
     7921 cacatttttt tcaatcaatt ggtatttgtt ttgatcgcca ttgtagaatt ttttgtttgc
     7981 cttccagtgt ccgtctttgt cagtttttac tgaatccaag acttctcctt ttttcacaag
     8041 gactgtttct ttatctggcg cagtaatatc tgttttggcg ataatatcaa attcgatatt
     8101 ttctgcagga acggtatcat aaacaggttt gccttgattc caggattctt ccgcattata
     8161 gacttctttg actttctttc cttctggaat gaactgttgt aagtcatttt ttgcagttaa
     8221 cgtcacttct ttgtttggtt caatcgtaat cgtatgagtt tgtttgtcta gaatataccc
     8281 agctggtgct ttgacttctt tgacatccaa tttattttga acatattttc cgtctttttc
     8341 atagaatgca gggatttctt ttaaggcgat ttcccctttg gcatccgttg tatattgttt
     8401 tgtttctccc gtatcatgac gtttgatttc atacgtcaca tttggaactt ttttccctgt
     8461 gacagcgtct agtttggtta gtttgaagtc gccatttttt agaactttaa tagttagtcc
     8521 agctctatta ggatctggca cataaagctt tcctagattt tgagcaacag gatctacata
     8581 ggcaaaactt gaacctaccc ctctagaatt tggatccagt gaagaaacag aaattcgtcc
     8641 gtcatttgag ttttgatttg cttttataaa caaactattt cctgattttc ttacttgaac
     8701 gttagctgtg ttttcaacaa ccgacccatc ccattgagaa aacacacctt ttgtatcctt
     8761 aacttctact tcttccccta ctttaaccgt cactgtttgg ctatcaaaag aaggatcttc
     8821 aagtaaattg tttatttttc tatctacagt ctgtttgaaa gcttcataat tagattgtcc
     8881 aatagttcca taattaacaa atgaaccacc taaaacttcc caaatatatg tttgtgctaa
     8941 tacatagtta tccattgaac cactagaata gtagccaaaa taaccaatta aagaaatctt
     9001 ttttctaata gcacttgtcg taattaaatt ttcgagtggt gtcacatcat attgaatacc
     9061 cgcactcgtg attgcgcctt cttgaatgca aaaaacatct tctccgtcaa tttgccagcg
     9121 aaccatgtaa tcatgagtat acattgttcc ttcaggagta attcgagtca tattcacatc
     9181 tttacgacca ttttcagtca atgttagatt ggtagcacgt gtctgtgcat tcttttccaa
     9241 gattgcttgc cattcttcag attcttgcgc caatcgttct tgttcagaaa aagaagtcgc
     9301 tttttgaata tcttcaatgg ttataaagta tttctcataa ccttcaggga cctcttgagt
     9361 cattacattg ggcggagaaa catcttctgt cgtgtcctgc gtagttgtct cttctgttgt
     9421 ttcacttgtg gtttcagtac tgatcgcttc ttcactactt tttgattctt cagtcttcat
     9481 tacttcttca gtagaatttg attcatttat cgtttctgca aatgccgtta cagatcctaa
     9541 catgggattg agaaatgtaa cgaaaattgc caaccaagtt gccattcttt taaacatatt
     9601 tttttcgttc ctttcatttt atttttcagt cttctaaagt aatttcttat ggacttcgac
     9661 accgaaacat ttttatcctc ctcaaaacaa aaaaagacta gaacttataa ttctagtctt
     9721 catcaaccac ttacttttct tttattttta gtagaaatct acagtgtgtt tttgtgaccc
     9781 gtcactgtaa ataataacat ccaccataaa acctttgttg taccactgcg aatttggatc
     9841 gctaacctct gcattagccc aatcataggc ttcatctaca gtgttaaata cctttccact
     9901 attaccaatc tctccaacta caattgttgg ctcttcaggt gtcgttgtgc caccatttcc
     9961 gccttgatcg cctccaccag gattgcttgg ttcttcaggc gttgttgttc ctccacctgt
    10021 gcttgggtta gaaggagttg tttctcctcc agtatttcca ccattacctg gttgtgtagg
    10081 tgtttgagta gtgccaccat tgttttctgg tgtacttggc gttgttgtac caccagttgt
    10141 tcctgtttga tctccagctg gtgttgttgg tgtcgttgta tttggtgtcg tttgtgtatt
    10201 tgaagtcgtt ccttgtccta ctggttgatt cggatcaact ttgtctttct cttgaatcac
    10261 tgtttgagcc acagtttcac ctgcgctgtt ttgtacactt gctacattgc cattaaatac
    10321 taatttattt cctgcataga ttaaatccac atttgtaatc ttattcatgt ccgctaattt
    10381 ttggacagta atatttgttg cttgactgat tgcgcttagg gtatctcccc agacaattgt
    10441 gtattcattc cctttaattt ctgctttgat ttgttccgcc gaacgaggtg tccaaacttt
    10501 gttttctgct gcttcagcat tcgttcccac agcaagacta actcctccga ctacgattcc
    10561 tagtgccaat actttcaaca ttttcattgt ttttcgcctc cgatttgaat aaatttattt
    10621 acaaaaacat agtaccaaac ttatgtctat cagtcgatat attttttgta gattttttta
    10681 ttttttgata tactaacttt ggtactaaaa taactaaaag gaatgtcaca catgcaactt
    10741 aataatttaa atgaaataca tgattgggct caaaataatt tacttaatcg taaacaagct
    10801 gccaaaatca ctggacaaaa ctatgatgct ttttctagct ctttacgtag aaataatatc
    10861 aaaccatttc tagaaatagg cgacgaacct ggtccttcca tcattcgctt atatctaaga
    10921 gaagatattg aaaaatacgc aaaacagctc gaagaaaaaa gacagaaacg tcaatgaggc
    10981 gttttctgtc ttggatttac aagtaaaaca ataataaaca cttttacaac ggaggttcta
    11041 tgaataagaa atacattgtt tcagttggtt caatcgtact gattgtactc gctattattg
    11101 ctttttctgt atccaacaaa acagaaacac agccacaaaa tacggttcaa tcgactacta
    11161 caagtcaatc tagcgatcat tccgtctatc cttcactaaa tagtttaaaa gaaacgcatg
    11221 gacaagattt aattgatctt ggttcttctc aaattactac tgtcttattt gaaaagaaaa
    11281 atattgcgtt tattactcct aaagaaggaa ctgaccttga attttctatc aaagtaacaa
    11341 ataccaaaga aaatttattc tccattcctg acattcaata tacccctaac aagttaaaga
    11401 ttggggatac gtttggtttg gctaaagtaa atgaacaata ctttgcttac tcactgaaat
    11461 aagtttctaa tagcaagagg aaaaaccctc ttgttatttt tatcacataa tcattttata
    11521 aaaacgcttc gaaaatatca acatttttga agaaactatt tttctaattt ctcaatttct
    11581 tccaagggat tttcgctaat gacccataca ggcacagatt gctctcgagg ctccccttct
    11641 tctaaccacc aacctgcata atgaacttct tcctcctcag cattaccatg ataccaacca
    11701 acaaaggatt catctacaac gaatgaaatt tcttcttctg gatcaaataa ttcatttttc
    11761 catgcttcaa ttgcttgttc tttggtgtac ttttgtttgt taaatacacc acaaatttcg
    11821 tcatctacat aaaaaatttc aaaatcaaaa ttactcgttc cttttactgg catcttaact
    11881 tttcctcctt tgactcactt caatagatat accactcatg ttcattccat tgtttactag
    11941 ttaattggag ttttatctta gggctttgtg cactctttcc aaaaacttga gcgtcgtttt
    12001 tttcttctaa ccaaaaaagg taatttctta cattcggtac taaatgtttt tcctgcattt
    12061 cttcatgaaa aacatataat tctgcttcat ttttcaacca attcattatt cattccctca
    12121 ttcacttctt cataccgcca ttcagatagt gagatttctt tttgtttctc cacttgtcct
    12181 ataaaaatcg ctgtattcaa caagctgatc acacttaata ataaagcaat gaaactaatg
    12241 gttacactta ctctttcaca ttttgtcatt ttgtttcctt ctttactcgt ttgttatttc
    12301 ttgtaatagg tctaataact ccttcaccga aatttgcaaa tggttcgcaa aaatttcaat
    12361 aaaagcactc gcacacatta ccagaccttc aactgatata tcactgcgac aataaaattc
    12421 tggagaaaca ctgatttctg attctttctt acaattgaac gaaaaaacta tttttgaatc
    12481 aaattccggt aattcttcca caactatgtt ttgggattct accaagaaag ggagtgcctg
    12541 ttctaatgct tcttctggtg tttgagcaga tagaataatt gcttcttgtt cttttatttc
    12601 attgttctca aatactttat aacttttttt cattgttagt tcctctttca ttcctctctc
    12661 aattagctaa gccgttactg gtacgccatt ctctaaattt agtatcgcta atccttaatg
    12721 ctcgtcgaat atcaatcatt cgataaccta ttgcacgaag ttctttatat cttttagcag
    12781 ttagcgtctc taacgtcatt tcaggcaatg ggttcgaatc aaaaatagaa ttcgaaccga
    12841 ttatttgggc caattgttgt acttgtaaca gctcttctga acttaattca gtgattgctc
    12901 gattgcctat cattgcatac attttctgtc gaatatactt cttttcttgt ttagacaaca
    12961 tttctatcca ctcctatttt cattatcatt tgttacctcc acttcagtaa atccacaaat
    13021 aaatgaattc atattccaag taccattccc ttcaactaca acttttctcc tgtcctcttt
    13081 cggatattca agaatcagac cgtttaccaa tatgtttttc actactacta atttgtttgt
    13141 ataccgtgga attgctgttc cgatatactt cactttattt cctacgctaa ttgtcatttc
    13201 ctcactccta ttctttttta tccaaaactt tttcctgtcc tattcctgcc aacgaatata
    13261 cttcactgca tgcttaaaca aaacaaaatg ttgccctgtt ttatttccgt cttttacttg
    13321 ttccacatac agttcatatt tggtcacacc ttttaaaacg ccagatactt tttttcatcc
    13381 attgtcacaa ttagtttttt catcaatcga ttgatactgc actgctcatt tttcttgttc
    13441 atatagtatt cctttctttt tcaaaactag cctcttttgt ttactagttt tcatcaatca
    13501 tcaacgattt aacagatttt ttctttcgca gggtttctag cgtcagcaac cccaaactct
    13561 aaaaaagtgc ctttgctccc ttgaaaaacg agcggacgat tgtactcgtc atcatatgtt
    13621 tttcatacta cctgatccac gcaaataacc tttttttgtt ctgtgccaca cccatccata
    13681 cgtatcgctt gagattcacc atactttttt aggtggttta tccttcctaa gtccctgtta
    13741 gacacgctga ttaacttaga aagtcctgtt tgtatccaat tttcaaagat ctcccaaaag
    13801 aaataagtag gtttacgtgt atcgcttctg ttcaaagtac ctgtttgtac ttgagtattt
    13861 ctactcccct cgttcaaggg attccttcca ccagctagct tttcctttca tcatcaaatg
    13921 ttcctgaaaa gcatttacgt ttcgtgtccg tgtgcttact tattcttttt tcgatagcga
    13981 ttatccgctc gctactcgct attttatact ttgtttgtta gctcaaataa aaactaacaa
    14041 acaaaaaagg ctagtttctt tccgttttat ttttttaact gtcgataaat agttgttagt
    14101 ttaattccaa gttctaaaac caatacgccc aacaaaacca caggaaaaac aattggggca
    14161 aacaagtaaa atcggatagt tttcatgtct tccctctttc tttttggtgt cgctgtggtc
    14221 gttcttgccg ttcaatactt cgatacattt tttattcatt atttgctcgt tttgttttcc
    14281 tctggactgc ttttcttgct ttttgaatac tttatacgct tttcgtgcct gaattaatag
    14341 gtcttgtcgg cgtttcattc gtgagaaaac aatatttgaa accatcaaat cctggttcga
    14401 acaaccacga ttcgcaataa ttttcccatc aaaaaaaata gttttcactt gagaaaaaaa
    14461 cagttggaaa tcttgttttg ttgctaattt ttttgtcaaa atcactgtgc ttttccctgt
    14521 tgaatctaca atgattcttc gaacaatccg attcgataac ttccaacgtt gccgattaaa
    14581 caatgttact tcacaacaaa tatgacgtaa gtgattaggg gtcattttca tgtgtccaat
    14641 tcctccaacc aattatgcat cgaaattttg ggcaattgtg acagcccata aatctgcgtt
    14701 tccaggtctt cataactaca agaacgcttg gcttgaagta atgaatattc tgcttgatct
    14761 gatagttttg ttgccattgg atctagattc aattctctta cacaacattc ccaaaaattc
    14821 aatagcgctc taaaataaaa tccttttcga ttctgaattg cttttccttc atgacgagca
    14881 attttttcat tttgaataca ccgttgatac gttttgctta aatcttctgc aaatgtttcc
    14941 cctaaaattc gatactcgct tccatcagat aaaccatgtg tttgattata ctttcgttgc
    15001 cgcttttcta taattcgttt actgtcgtat atcatgttta gctgctcttt cgcttcacgc
    15061 cattcagctg aactgcgaac aatccatgtc aaaacatctt tcggaacaaa cgtactttcg
    15121 ctgtaatgag caataaactc tttttgccaa tcatcagcca tttcaacagg actagtcgta
    15181 tcgatttttg tatctagatt ttctgtatct agattgttag aagaattatt aaataattgt
    15241 gcagaatttt cacatggatt tttttcgtca aaatcatcga aaatcgctaa attatcaaca
    15301 gattcctcct cttcttttcc ttgtgcagaa tctacactgg ttgagtcaac aggacctaat
    15361 gaatacacat cattttctgt cgtatctaat tctcctagat acaaccgagc tttgtttctt
    15421 tttcctgttt gttgatcaaa tcccatatcc tctatttcta gtagctcata ttctttcaat
    15481 tcttttttga ttgtattaac agaacgtctg caacaattca atgcattggc taattcttgt
    15541 tccgtaaatt caaaataaac aaaaccttga ctatctactt gatttcgttg aatggctaaa
    15601 tctaagcgtg accgaaaaac catatacgcg atttttgctt tatcagacaa tgagcgatat
    15661 ttttcacttg taataaatac ctttggaaat tgatagtata attcattgta tttacgagtt
    15721 gcggtaaatc tttttgttgc catcgttatt acacctcatc ttgtccatat attaagaaag
    15781 aattttggtg ttggtagaca cgaacatctt ccattgtagc aatcaattct tttagataaa
    15841 ttcgaccgaa ttcaattgtt agtaagtcgt aatcttctaa ttcatttttg atttcttcaa
    15901 tttcttcttc agacacattg agtaaaatgg ctaattcttg attggtataa gaaatataga
    15961 catgtttctt ttcatcaatc cgtttttcaa ttaaggattc ttctaagtac tcattcagta
    16021 agctataggc gtattttgct tgtgcagtca tcgcataata ttttgacgat tttataaaga
    16081 atagttttgg catatgaaaa gttgcttgtg tcagcacatc tgttgcgcga aatttgtttt
    16141 ttaccatatt ttttcctcct agaaaaacac gcaaaaaaag ctctgtataa atcgaattac
    16201 acagagtttc ttcttgttga tatttaattt ctaaaatacg tttcgggtaa aaactaaaca
    16261 cttactaata tttagattgt agaatcttcg ccttcattta ttcattttaa agagaaaaag
    16321 atcaagtgaa atgtgcccaa aagtttgatt ctattaattt ttaaacacca ttcataatat
    16381 atttcctata atgtaaaaag cgtcgatact tattaaagca tcgacgttat ttttatagta
    16441 aatttatcaa aatttttgcc aacatttttt ccgatttttt tccctatttc tatctttcct
    16501 tatctttctt agttaatatt atttgatgtt aatatatcac tactaatatt aaaaaacatt
    16561 gatttatcat aggataaaga gtgatagttt aatacttaaa gagacatatt aattttattt
    16621 aaaaattgac attaatgact cttaatcagc gggtcgcggg ttcgagcccc tcacggccca
    16681 ttgggtgcca aacccacgga acaggtaaat gttggtgcga aagcatttcg aaaacattta
    16741 cgctgttctt tttttatttt tcaaaataac tagggctttt tatttttaac tcaaagtcca
    16801 ctgtagccct ttggactttg agttaaaaaa gaagcataat tatgaacagt aaagtttcta
    16861 atataacaat aattcgagaa gctttgcaaa atcatgctgc caatgaactc tctaaa
//
LOCUS       DARBEC010000059        16376 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.060, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000059 DARBEC010000000
VERSION     DARBEC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16376)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16376)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16376
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.060"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            135..2606
                     /locus_tag="SCL80_001884"
     CDS             135..2606
                     /locus_tag="SCL80_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1107742.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(2709..4445)
                     /locus_tag="SCL80_001885"
     CDS             complement(2709..4445)
                     /locus_tag="SCL80_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEG1107743.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(4442..5146)
                     /locus_tag="SCL80_001886"
     CDS             complement(4442..5146)
                     /locus_tag="SCL80_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107744.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5277..5780
                     /locus_tag="SCL80_001887"
     CDS             5277..5780
                     /locus_tag="SCL80_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEG1107745.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            5740..6078
                     /locus_tag="SCL80_001888"
     CDS             5740..6078
                     /locus_tag="SCL80_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEG1107746.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6099..7517
                     /gene="ffh"
                     /locus_tag="SCL80_001889"
     CDS             6099..7517
                     /gene="ffh"
                     /locus_tag="SCL80_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEG1107747.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            7629..8207
                     /locus_tag="SCL80_001890"
     CDS             7629..8207
                     /locus_tag="SCL80_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEG1107748.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8211..8732
                     /locus_tag="SCL80_001891"
     CDS             8211..8732
                     /locus_tag="SCL80_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEG1107749.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(8811..9365)
                     /locus_tag="SCL80_001892"
     CDS             complement(8811..9365)
                     /locus_tag="SCL80_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1107750.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPGGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            9618..9893
                     /gene="rpsP"
                     /locus_tag="SCL80_001893"
     CDS             9618..9893
                     /gene="rpsP"
                     /locus_tag="SCL80_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEG1107751.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            9905..10156
                     /locus_tag="SCL80_001894"
     CDS             9905..10156
                     /locus_tag="SCL80_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEG1107752.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10281..11072
                     /locus_tag="SCL80_001895"
     CDS             10281..11072
                     /locus_tag="SCL80_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107753.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11242..11763
                     /gene="rimM"
                     /locus_tag="SCL80_001896"
     CDS             11242..11763
                     /gene="rimM"
                     /locus_tag="SCL80_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEG1107754.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            11753..12514
                     /gene="trmD"
                     /locus_tag="SCL80_001897"
     CDS             11753..12514
                     /gene="trmD"
                     /locus_tag="SCL80_001897"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEG1107755.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            12650..12997
                     /gene="rplS"
                     /locus_tag="SCL80_001898"
     CDS             12650..12997
                     /gene="rplS"
                     /locus_tag="SCL80_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEG1107756.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13129..14265)
                     /locus_tag="SCL80_001899"
     CDS             complement(13129..14265)
                     /locus_tag="SCL80_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1107757.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPDLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKIPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVNFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPNGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(14381..15295)
                     /locus_tag="SCL80_001900"
     CDS             complement(14381..15295)
                     /locus_tag="SCL80_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEG1107758.1"
                     /translation="MKKIVFGLLFLGLVLSGCSTSPNSASTKENTVSSTSEYETKKLT
                     SSDVHKIKVGDDLTQVYQTLGSPLRKWTNDFVYKELDNAITRDELIVSISKDAQGDYY
                     KEHSELIEHGNSAKDIKKLEMLQYTYSTDSDDETMLIWVSPKTEQVVYYNQRTFLDKD
                     GKSKDSSSTTTTTTTSSSEPQTASVAHSIGEAVPFTSENGNSINVTVQSVSKDYGDDF
                     YKPEGLFYAKVDFLVENTGTTPFDASSSYLEFYDSSDIKSDLASRDYFSETIQPGKSA
                     QGTAYFEVKNDGTTFEVFFADTSWKGSY"
     gene            complement(15386..15814)
                     /locus_tag="SCL80_001901"
     CDS             complement(15386..15814)
                     /locus_tag="SCL80_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEG1107759.1"
                     /translation="MEMDTINLVEELKRKYQSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKRIEELKNLYGLPESAYRFLI"
     gene            complement(15819..16193)
                     /locus_tag="SCL80_001902"
     CDS             complement(15819..16193)
                     /locus_tag="SCL80_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107760.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
BASE COUNT         5279 a         2952 c         3219 g         4926 t
ORIGIN      
        1 cctttttttt tgctatatga caggaatgaa aggtgtttgc aagcttaatg tgaggaacac
       61 tttccatatc tgttctatac tggataggag aagtttggaa atatccagaa aacgaaatca
      121 atcgaggtag ccacatgaag aataaatgga taaatcgaag taaaaaaaag aaagtaacac
      181 aaagatcatc agataaagtc atccgtcttt ttccatcgta taaagaagga ttaacggcag
      241 atcaagtcaa ggaacgaata gaaaaaggtg cagcaaataa ttcagtcgat cccacattca
      301 aaacaaatca gcaaatcgta ttggaaaaca tatttacgta tttcaatttg atctttctta
      361 tcttagccat tttgctttgt ttagtagaat cctataagaa cttgactttt ttaccagtta
      421 tcatagccaa tacaggaatc gccatttttc aggaaattcg atccaaaaag atcttggacg
      481 aactaaatgt tttaaataca gcaaaagtca atgtcatacg agacgggata gaacaaacaa
      541 tagatataga agaccttgta atagatgaca ttgttatttt agagactgga tatcaaattc
      601 cagcagatgc agaggtcgtg gaagggaaac tgcaagttaa tgaggcttta ttgacaggcg
      661 aagcagatga aataaccaaa gaaataggtg atccattaat gtccggcagt ttcattgttt
      721 ccggaaaagc ttacgcccga ttagacaaag ttggagcaga ttcgtatatt tcccagctga
      781 cagtcaaagc caagacaatg ggagaaggag aacagtcgga gatgattgct tctcttaatc
      841 aattgatcaa atggattggg atcatcatta ttcctatcgg tatcctgctt ttttctcaaa
      901 gttacttttt taatggaaac acgatcaaag aaagtgtcgt tgcaatggaa gcagcattga
      961 tcggtatgat acctgaagga ctgtacttgc tgacgacgat tgcattagca ttaagtgcga
     1021 ctaaactagc aaagcaaaga gttcttttac acaatatgaa aagcattgag acattggcaa
     1081 gggtaaatgt cctttgtgta gataaaacag gaacgatcac agaaaataaa atgtctgttc
     1141 aaaaaatgat cgtctctaaa aaacaagaaa taatcagtca gtcagatcaa ttggaagcat
     1201 gcattgctga ctatgcaaaa gcgatggcag gagacaatgc aacaatggaa gctgttaaag
     1261 catattttac aaaaaccact gatcaatcct atcaccatgt tattcctttt tcttctgtcc
     1321 aaaaattcag cagtgtagcg ttggcagaca aggtgtatgt tttaggagcg cccgaaatgg
     1381 ttttgcgcga tcaattgccg gaatatgcgg aagagtttgt ttctttcgct gaaaaaggct
     1441 atcgcgtgtt ggtctttggt gtgtacgatg ggattcttga agaaccagta ttgacagaag
     1501 cagtcacacc attaggctat attctgattg ctaatcctat cagaaaagaa gcaagagcca
     1561 catttgatta cttcaaaaaa cagcaagtag ccatcaaagt gatttctgga gataatccgc
     1621 tcaccgtatc caatgtagct gtccaagcag ggatttcaca tgctgatcag tacgttgatg
     1681 catctaaatt atcagaagaa gaatatgaag cagctgtcga aaaatataca gtttttggac
     1741 gcgtcaagcc agaacaaaag aaaatcttcg ttcaattgat aaagcgtaac aatacagtag
     1801 ctatgacagg tgatggcgtg aatgatattc tagccatgaa agaagcagac tgcagtatag
     1861 cgatggcttc cggaaatgaa gccgccatgc aggcctcaca agtggtctta cttgattctg
     1921 acttttcacg catgccagaa gttgttgcag aaggacggcg cgttgtgaac aatattgagc
     1981 gatcagccag cttgttttta gtgaagaata ttttctcctt tttgctctca ctgttttcag
     2041 tgatctttgc tctgacctat cctttagaac catcacaaat cacattgatt agtttgttta
     2101 ctatcggact tccatctttc ttgcttgctc tagaagaaaa caaaaagcga atcagaggga
     2161 aattcatcat gaatgtgatg gaaaaagctg tgccaggtgg actaacagat atgatcgtag
     2221 tcggtgcgtt ggtgatctgc ggggtgactt tgaatctaaa taaaacagat gtttctacag
     2281 catctaccat gcttttgatt gcagtagggt tcctcgttct ctataaaatc tgttcaccgc
     2341 tgaataaatt cagaagtcag atcattcttt tctgtgctag cggtaacttt ttttcggtca
     2401 tctttttgca taagctgttt tcaatcaccg gtatctcagc tgtatcactt ttgctgttga
     2461 gcattctatt tttctcggca gattcgattt ttaggcattt gaccacattg gtagaatatt
     2521 tgtttgaacg taaaaatgaa aaagagcaaa agccgtttag tcgcgtagct aaaaaatttc
     2581 tatcgatatt taaacgagaa aattgacgaa aagtatctca aaaaagagta aaaaagaaca
     2641 accactcgtg tatctttcga agcaggatga gtcatccaac tttaaaacgc acgagcagtt
     2701 gctctttttt acattttcgg aatggtaacg gtgaaagtcg aacctgttcc aagatgactg
     2761 tctacagtaa tcgtaccatt aaggagttgc acgtagtctt ttacaatcgc taaccctaat
     2821 ccggtcccgc ctgaatgtcg gcttcgcgcc ttgtctacgc ggtaaaaacg ctcaaagatc
     2881 cgttcctgat cctcaatgtc gatacctata ccaaagtctt ggacagaaaa cgatagatca
     2941 tctgttgcct gatagcggat aataatctcg ctgtcgcttt ttgaatattg gacagcattt
     3001 tcaattagat ttttgataat tggataaagg atatctgttt tagtagtaaa tggttcgttc
     3061 tttccaatca atcgaatcgt caaccgtttc ttttcgatga ttggctgata attttgaacg
     3121 atttgctgga aataaggttc tgtataaatt gtctggatct cgtaagaatt ctcgccatct
     3181 tttgacagct ggatgatttc ccgaatcagt ttatctaacc ggatcgcatc tttttgcatg
     3241 atctcaagaa aagaagtcag agtttgcggg tcatcttttg caccatccag taaagtctcg
     3301 gtaaaaccaa tcagtgaagt cacaggagtt tttaattcgt gagagacgtt gccaacgaaa
     3361 tccttttgta gcttttctaa acgtcgcact ttcgttaagt catacgccac ccctagaatc
     3421 tgtccggtac cttctgaatt agtaaagtag cgcaaactca agtctaatgt ctgccgacct
     3481 tctgtaaggg tgatctcctg atggatcagc ggatgctcag gagtcacttg atgaatcagc
     3541 tgaatgagtt ttggctcttg aatgacttct gtgtaccgct gttcaggttg atagcttacc
     3601 acgtttaaat gtagctgcat ctttggatta agcagtagta aacgagaatc agcatcatca
     3661 atcaggaata cgccaatcat cagttcgttc agtaacgtat ataattgctc ttctgtggta
     3721 gtacacgccc gatacatctt actcatttgt tcgctcaacg cattaatcgt ctgatacaat
     3781 tcttcccatt gatcgggtgt ttgcatgatt acttccgcct gattaggatt ttgcaccatt
     3841 ttttttagta cgggcagcac ggttttcaat ggctggtttt tttgatagat cagataatag
     3901 atcattcctg caagaacaag gaagaaagac agaaagaaga aaaagaccca acggcgaaaa
     3961 ctctcagttc tcggtaaaaa accgcttgtc ggttctgcga tacgtaaaat ggcttctaac
     4021 tggccacttt ttttgactgg taatgcgaca tataaaagat cctcattcaa agtagtactt
     4081 ttgcgcaaag cgctacctag gttcccacca ttcaatacgg ctttgatttc gggacgggag
     4141 tttcgctgtt cattcaatgt ttcattgttt gtgtcaaata agatctttcc agtagcatcc
     4201 attaaagtaa tccgttcgtt cgactgatgg acgaattctt ccaaagcagt tttgttttgc
     4261 gctgcttgga ggttttcgac atctaattgc cggatcagca gttgtgcttt ttttgtcagg
     4321 tagctttctt gctgttccac gatctgctgt tgaaaataat gggaaatcaa ttgccagcca
     4381 ccaaaaaaca aactaactaa aagaataaga aagaagagac gttcataaat ttttttccat
     4441 ttcatcgttt gggttcctga aacttatagc caaatcctct aatagtcacc aagtactttg
     4501 ggtgttttgg atctcgctcg attttttctc gcaaatgact aacgtgtaca tctacgattc
     4561 ggctttgtcc tgcgaagtca aaattccaaa tgcggtctag caatgtgtct cggtcgatca
     4621 cacggtcttt tcgtttcatg aaataaacaa gtaattcaaa ttctttcggt gttagttcaa
     4681 tcagatggtc gtctacggtc acttgataat tggtcaaatc agcagtgatt tgtccaatcg
     4741 atagatattc aggctcagct tcatcttttg attgcttacg aggttcaatt cgtctgaata
     4801 ttgctttcat acgggctaat acttcgcgag gactaaatgg tttggtcaaa tagtcgtcag
     4861 cacctatttc taggccaatg atccgatcca cttggtcatc tttagccgtc agcatcaaaa
     4921 tcggtgtttc atttttttct tgacgcagcc gctgagtgat ctccatgcca tccatcgttg
     4981 gaagcatcac atctaaaatg atgaaatcaa atgattgttc taatgctaat tctaatcctt
     5041 cgcctccatc tgtggcactg gttaccttgt agccttcttt ttccaaattg aacgtcaaga
     5101 gcgttacaat agaaggttca tcatcaacaa ctagtatttt tttcattcaa atctccttta
     5161 aataggtttc attctgttta tattttagca tagaatcagt acaaagggct attttacaaa
     5221 agaaataaat ggtataatag gaaggcactt tgctgaatag aaaggaagag cagtcaatga
     5281 ttggaatcag tgcatgttta ggcggtctgg cctgtcgcta tgatggacag aaaaaagaag
     5341 taccagaatt gatggaacta gttgaaactg gagaagctgt catggtctgt ccagaagtga
     5401 tcggcggatt accaattcct agagatcccg ctgaaatcat tggtggaaat ggattcgatg
     5461 tgtggaaaga tcaagcaaaa gtatggacga caagcggcga agatgtaacg gaagcgtaca
     5521 aaaatggcgc gatcaaagcc tatcaaaaat tgcaggaaaa acacattgat ctagtcatca
     5581 tgaaggaaaa aagcccttca tgcggaaccc attcgattta tgacggaagt ttttccggaa
     5641 taaaaaaaaa tggtgccgga gtggctactg cttattttat tcagcaaaaa atgactgtgc
     5701 tctctgatga agaatggctg aaaactagag gtgagcagaa tggaaatcga aaagaccaat
     5761 cgaatgaacg cgctctttga attttattcg accttattga cagaaaaaca aatgaactac
     5821 atggaattat attatgcaga tgatttttca ttaggtgaga tcgcggaaga atacgaagta
     5881 agccgacaag cagtatatga caatatcaag cggacaagca agatcttgga ggattatgaa
     5941 aaaaaactcc atcttttttc taattatatt gttcgagaac aggtattgga aaatatgact
     6001 gcatatatta cagataagta cccggaagat aaaaaactac ttgaatatgt acagcaaatt
     6061 caagcaatcg aagaataaat aaaaggaatg aagaaaatat ggcatttgaa agcttaactg
     6121 aacgtctcca gcaggcgatg ggaaaaataa gaaaaaaagg aaaagtatca gaagctgacg
     6181 taaaagaaat gatgcgggaa atccgccttg ccttactaga agccgatgtt aacttgcaag
     6241 tagtcaaaga ttttactaag cgcgtacgcg aacgtgcggt aggggcagaa gtgctggaaa
     6301 gtttatcacc tgcacaacaa atcgtaaaaa tcgttgatga agaattgacg atcacacttg
     6361 gttctgaaac agcagagtta aataaatcac caaaaatacc aactgttatc atgatggctg
     6421 gtcttcaagg ggcaggtaaa acgaccttta ctggtaaatt agccaattat ttaaagaaaa
     6481 atgaaaatgc tcgtccgctc ctaatcgcag gggacgtata tcgaccagct gcgatcgacc
     6541 aattgaaagt attaggtcaa cagctagacg ttcctgtatt tgatatggga actgaagtca
     6601 gcccggtaga gatcgtccgt caagggatgg agttagcaaa agaaaagaaa aacgactatg
     6661 tattgatcga tacggcagga cgtctgcata tcgatgaaac attgatggat gagttgaaac
     6721 aaatcaaaga attgacgcaa ccaaatgaga ttttgcttgt tgtcgatgcc atgactggtc
     6781 aagatgccgt caacgtagct gatagcttca accagcaatt aggtattaca ggggtcgtca
     6841 tcactaaact tgatggggat actcgaggcg gggcagcact atcgatccgc tcagtgacag
     6901 gcgcaccaat caaattcatc ggttctggtg aaaaattgac tgacttagaa gtattccacc
     6961 cagatcgtat ggctagccgt atccttggta tgggggatat gctgacattg attgaaaaag
     7021 cgcagcaaga ctacgacgag aaaaaagcgg aagaacttgc acagaaaatg cgggaaaatt
     7081 cattcgattt caatgacttt attgaacagc ttgatcaagt gatgggtatg ggcccactgg
     7141 aagatctaat caaaatgatc ccaggaatga atcaagtccc tggaatcgaa aacgtcaagg
     7201 tagatccaaa agatgttgaa cgcaaaaaag cgatggttta ttctatgacg ccagcagaaa
     7261 gagcaaatcc tgatctgttg aacccaagcc gtcgtcgccg aattgctgcc ggttctggaa
     7321 acagtgttgt tgaagtgaat cgaatgatca aacaattcaa ggaatcaaga aaaatgatgc
     7381 agcaaatgtc caaaggggat atgaacatcc caggtatgga ccaaatgttt ggttcaggcg
     7441 tcaaaggaaa actaggcaaa atggcgatga atcgaatgat caagaaaaat aagaagaaaa
     7501 agaaaaaacg gaaataagat gaaaaaaaca gcttcggtac agactgaaaa tgtctactgc
     7561 cggagctttt ctatcagcaa tttttattct ttactaatac aattactcta aacgaagagg
     7621 tggcacagat gagatgtccg tggagtgaac aaacagaaag tatgcggcaa tatcatgata
     7681 ctgtctgggg tgttcctgaa tatgatgacc aaaagctgtt ccgaaaattg atgcttgata
     7741 ttaatcaagc cggattgagc tggcagacga ttttaaataa aagtgaagca ttcgatgaag
     7801 catatgatcg attcgatatc aaaaaagtcg caaattatgg agaaacaaaa atcagtgaac
     7861 taatggaaaa caaaggaatt attcgtaacc gaagaaaaat cgaagcagcc gtccaaaacg
     7921 ccaaagcagt cttgaaaatt caagaagaaa tcggcagttt ttcagagtat ctttggtcgt
     7981 tttctaatgg aaaaatcgtt gattcttctt atcattcaca agaagaagtc ccaacaacaa
     8041 gtcctctttc agatgaatta gcaaaagatc taaagaaaag aggatttaaa tttatcggaa
     8101 gcacaacaat ctatgctttt ttagaagctg tcgggatcat caatgatcac cttgacagtt
     8161 gtttccgcaa agaagaggtc gtacgtatcg gcagaaaggg cgaataaagc atgcgaaaaa
     8221 tcatatttta tggtgctata agtttagatg gctatttggc aacgaaaacg gatgatcttc
     8281 aatggctttt tgacactcca acaggtaagc aaactactta tgaagctttt tatgaaacaa
     8341 tcgatacgac catcatgggc agaaaaactt atcaggaagc aaaaaaatat ttggagaccg
     8401 aagcaattta tccagagaaa aagaatttcg ttttttccac ccacgcacaa ttacaattgc
     8461 cagatgctga ggtcgtaaac gaagatcctg ttgcttttct aaaaaatcta caaatgacag
     8521 aaggaaaaga tatctgggtt gtgggtggcg gtcgattgat caaaccgcta ttggaacatc
     8581 aaatgattga tgaatggtat atccagatta cccctgtatt attaggggac gggattcctt
     8641 tattccaaaa aggaacatat gaagcgcgtt ttgaaatgct agacacgaca cgattcggtg
     8701 aatttatcga attgcattat gtacgaaagt agaagtaaat aaaataaaaa aggcaactat
     8761 atgcagaaat gtcctgtaca tagttgcctt ttttattacc gttatgcttt ttactctgtt
     8821 aatttgttag aaaaatctag atacgcatct aaaatcgttt ggaagaaact taatgttcct
     8881 tcaacgatat taccattttc atcaattaga ttcgcaacgt ttccgatata tgcttccggt
     8941 tgctgcaagg ttggcacatt caaaaagaca agtgattggc gtagatggtg gtttgcgcca
     9001 aagccgccga tcccacctgg tgatgcagta acgactaaac ctggtttatg atcccaaaca
     9061 ctgtgtccat aaggtcttga gcctacatct aaagcatttt tcaatactgc tggcacagaa
     9121 cggttgtatt caggagtaac gaaaacgaca ccgtctagtc ccctcacttc ctcacgaaaa
     9181 cgagtccatt cagcaggagc ctgttctgga gtttcgagat cctcattgta aaatggcaga
     9241 tcatcgattt ttacaaatac actttcgtac ccttcaggaa gcaatttcgc aaaagcttct
     9301 gctacttttt tgttgtaaga atcttttctt aaactaccaa taaaaaatcc aatttttttt
     9361 gtcatacgat ttttcctcct tcaaactatc tataataatc ttacaaaaga taagtaactg
     9421 gagcaaataa aaacacttac tgacaaaagc tacatagaag atagattact tatttttaac
     9481 aaaaaagaag tctaaaatct tctattttct aaaaaaaatc gaatttaatc aagctgtaaa
     9541 gaaaaaaagc tttacagggt aaaaaaaaac tgttacacta catcttgtga ataaaacaat
     9601 ggaggtggaa ataaataatg gcagttaaaa tccgtttaaa acgtatgggt tctaaaaaga
     9661 gtccgtttta ccgtatcgtc gtagctgatt cacgttctcc tcgtgatgga cgtttcatcg
     9721 aaactgtagg tacttacaat cctttgaaag accctgcaga agtagtttta aaagaagact
     9781 tagttcttga ctggttatca aaaggtgcac aaccttctga cactgtacgt aacatccttt
     9841 caaaagaagg cgttatgcaa aaacaccacg aagctaaatt ctcaaagaaa taaggtgacc
     9901 ggatatgaaa gatttaagcg acttagtctt aacaatcgtt cgtccgctag tcacttatcc
     9961 tgatcaagtc aagttagacg tcgtagagtc gaaagacttt tacgagtaca atctaacagt
    10021 ggctcctgaa gatattggcc gtatcattgg aaaacaaggg cgtgttgcta aagcaatccg
    10081 cacaatcgtt tacggtgttc ggatcaatgc accgaaaaaa gtgcgtttga atatcatcga
    10141 tgataaggaa aagtaaataa acgtgctgta ctcgaatttt cgagtgcagc ttttttgttt
    10201 tttttattaa ttgaagaaat gattatttgg caaaacaagc aagaaagcgt atccttatag
    10261 gaggagggag tgaacagcca atgagaaaga aaatgttatt tattccattt gctctattgt
    10321 taagcgcttg tagttcggtt actcaagaat cagacaatac acatctatca agcagtatat
    10381 ctgaaactgt taccagtcca gaaaaaagta gtaccaaaac ggcttccacc actgaacaaa
    10441 caatggctac ctcaaataat gagaaaaaga ctgctttaga ccagttgaaa gaacaacagc
    10501 caaatgttcc catgcctcta gatgtaccag tctcttcagg ctatctcaat atcgcagcga
    10561 cacatacaaa gcaaggttat tctattttat attacagaac agatcgtccc ctcgggttga
    10621 atgctgacga attgaatcaa gaaacgccta ttgcaaccta tctatatcaa tatgggtttg
    10681 cttctagtca agaaacaatc caagttttgc agccatttga gatcgacaca aatggtcagc
    10741 aagtggactt aggttctcga atcacagggt atcagcaagg tgcagcaggt tcaagttttc
    10801 ttgaatggca agaaggaaac tggcgtattc gaatccgtgg gaataatata gaaggccaag
    10861 atcccttact attagctaaa gaaatcgtag cttatttgga agaaaacagt ctaccagcac
    10921 ctgaacaatt tggtaaaatt actgttgata tgggtgatac gacgaatcga gccgtagaag
    10981 taagctggca agaaccgaaa aatgcctata cgatcactca tcaagatcct atgtctgcgt
    11041 tgaagatggc agtttctatg aagaggttgt gaaatcagcg cgctgatctg agcccatcat
    11101 ttaactccaa gtcacaggaa gtaatcaaaa atagcacgcc gcatttttgc ataattttgg
    11161 cttaatacct taaaagaaaa ctagaattgc ctcttcacac atgctaaaat aaaacagaca
    11221 aaaaagaaat ggggatataa gttgactgaa tacttgaatg taggaaaaat cgtaaacaca
    11281 caaggtatca aaggcgaagt ccgtgttatc tcaacaacgg atttcccaga agaacgttac
    11341 aaaaaaggaa cggtgctaac actattccaa gacggaaaag cacctgtgga attaacagtc
    11401 aaaagtcatc gcaaacataa aaattttgac ttattgagtt ttgaaggaca tccttctatc
    11461 aatgatgtgg aaaaatatcg ggatggcata ttaaaagtgt caaaagacaa ctctgtagat
    11521 ctagctgaaa atgaattcta ttaccatgag atcatcggtc tgcatgtatt agaagatgga
    11581 actgagttag gtaaagtaaa agaaatcttg tcaccaggtg ccaatgatgt ttgggtcgtt
    11641 caacgtccga aaaaagctga tatattgctt ccatatatcg cttctgtcgt caaagaagtc
    11701 gatttagaag ctggagtggt ccgtgtagaa attccagaag gactgattga cgatgaagat
    11761 tgatgtattg actttatttc caagaatgtt tgaaggtcct atgggggaat ccatcatcgg
    11821 taaggctgta gataaaggat tattagatat caatatctct aacttccgcg attattcaga
    11881 taacaaacat cagacagtag atgattatcc atacggcggt ggagcaggta tgctgctcaa
    11941 agtccagccc atctatgata acatccaagc cattgaaaaa gctgcaccgg atgtcaaaaa
    12001 aagagtaatt cttcttgacc cagctggtaa acgattcgat cagaaaatgg cagaagaatt
    12061 ttcacaagaa gaacacttag tattcatttg tggtcattat gagggatatg atgaacgtat
    12121 ccgcacatta gtgacagatg aagtttcctt aggagattat gtccttactg ggggagaatt
    12181 aggtgcgatg gtcatgatcg atgcaacagt tcggcttttg ccagatgtgt tagggaatca
    12241 gacttcagct caaacggatt cttattcaac cggtttattg gaacatcccc aatatactcg
    12301 cccggcagaa tataaaggga tgaaagtacc agaagtattg acaaatggaa atcataaatt
    12361 gattgaagaa tggcaattaa aagaatcact aagaaggacc tatcaacgtc ggcctgatct
    12421 tttggaaaca cttgaatgga cacctcaaat gacgaaattt ttagaagaaa tcaagaaaga
    12481 agaacaagaa aaaacagcag atgaagccaa gtgacttttt ctaaaatctt ctttacagta
    12541 ggttgataat atgttagtat tttatggtga gtgcaaaagc acttaactat tacgatattc
    12601 cgctgtgaga aaagctcata agaatatttg gaataaggag aagaaaacca tgaatccatt
    12661 aatcgaagaa ttaacaaaag aacaattacg ttctgacatc ccagcgttcc gccctggtga
    12721 cactgtgcgt gttcacgcaa aagttgttga aggtactcgt gaacgtatcc agttatttga
    12781 aggtgttgtt atcaaacgcc gcggtgctgg aatcagcgaa acttatacag tacgtaaagt
    12841 ttctaacgga gtgggcgttg aacgtacatt cccattgcat acaccacgtg ttgcaaaaat
    12901 cgaagtagtt cgctacggta aagtacgtcg tgcaaaattg tactacctac gtgcattaca
    12961 cggaaaagct gctcgtatca aagaaatccg tcgttaatca tcgttatcag actgagaaag
    13021 aacccttggt attcaagggt tctttttttg ttctctgttt ttttatatta acgatttcta
    13081 tttgggacat ttttgattaa taaaaagggg caaaaaaggg gcaacaagtt acagtttttc
    13141 tatatcttct ttcaattttt cttttactcc ctttgtcaca tgcaaataaa tcttttcagt
    13201 gatatcacta ttttcgtgcc caacgcgatc ctgaatagca tacagaggag ttcctagttc
    13261 cgctaatttt gaaatatggg tatgtctaaa aatatgtgaa ctaagttttt tatcaatctt
    13321 catgtcagct ttatgatttc tcaaataggt gttaattgct gttagttgga aaggagttcc
    13381 tttcgttgtt tgaaatagaa attgaccatt cggatttaat tctagaagct cattgtaaat
    13441 agctatcgct ttttttggta aatcaatttc tcgcattcca gcagcagttt ttgtagaatc
    13501 agattttttc atatcagcta ttgaacgttc tctatacatc atcgtcccat ttataactac
    13561 tgatgcatta ttattggtaa tgtgtacatc gtctttactt aatgcgattg cttctccagg
    13621 cctcattcca gtcaaatata gccactggaa aaggagagaa tatcttttat tgtgtgaagt
    13681 tgtgaaattt actagtcttt tatattcatc atcttctaaa aacttatctt taatttttat
    13741 cgtttttgac tctcgtttat aatcaataac tacttcgttg ataggatttt tttctacata
    13801 gccttttttc atagcgtatg aaaaaagaag attcaatttg gatttaatta cactgacgta
    13861 cttatttgac aagtcatctt tatatatcat atcttcaaaa atattattta aatctactgt
    13921 attaatacca gaaacgatgt aagtctcagg tatctttttc tttatagtgt ttaagatatt
    13981 attcgtagga taatatgtag actccttaac ttgtcttttg taaataacta accattcttc
    14041 gacaagctca tgaaatgtta gatcaggctt ctgaagtgtt ttttcgttta attttatatc
    14101 aattttttta tttaactcta gcaatgccac tttctgagtt tctcgagatt tatttttata
    14161 agtaatgctc actttttttc tttttctagt ttttggatca atatatcgct cattatattt
    14221 atatactttt tttccgtttt tatcagtttt ggtttcaatc cacattgttt tcatctccta
    14281 tttgttgcta taataggaat ggataagtaa gccaattata gcaggtttat ttttcatcac
    14341 gtccacaaac tttggcgagg gagggggcgt gtttttttgt ttagtagcta cctttccatg
    14401 aagtatctgc gaagaacact tcaaatgttg ttccatcatt tttcacttca aaatatgctg
    14461 ttccttgtgc ggatttaccc ggttggatgg tttccgaaaa gtaatctcta ctagctaaat
    14521 cagatttaat atctgatgaa tcataaaatt ctaaataact tgaacttgca tcaaaaggtg
    14581 tagtccctgt gttttctaca agaaaatcta cctttgcgta aaataggcct tctggtttat
    14641 agaaatcatc accgtaatct tttgatacag actggacagt tacgttaata gagttaccat
    14701 tttcagaagt aaaaggaact gcttctccta tactatgtgc aactgatgcc gtctggggtt
    14761 ctgaagacga agttgtagtt gtagttgtag tagagcttga atctttggat tttccatctt
    14821 tatctaagaa agttcgttga ttataatata caacctgttc agtttttgga ctaacccaaa
    14881 ttaacattgt ttcatcatca gaatcagttg aataagtata ttggagcatc tctaattttt
    14941 ttatatcttt tgcactattt ccatgttcaa ttaattcaga gtgctcttta taataatctc
    15001 cttgtgcatc ctttgaaata gatacaatta attcatcccg tgtgatagca ttgtctaatt
    15061 ctttgtaaac aaaatcattt gtccatttac gtaacggtga accaagtgtc tgataaactt
    15121 gagttaaatc atcaccaact tttattttat gaacgtctga tgaagttagt ttttttgttt
    15181 catattcaga tgttgatgaa acagtatttt ctttagtgct agcagaatta ggactagtgg
    15241 agcatccgct taataccaag cctaaaaaaa gcaacccaaa aacaattttt ttcattttta
    15301 attcctcact tctatgatat gatttttttg taggatctta gaaataaggt ttttagtccg
    15361 tgttgcagca cggacttttt ttacattata taagaaagcg ataagcgctt tctggaagtc
    15421 cgtaaagatt ctttaattcc tcaattcgtt taggatattg atcattgtct tctttgtata
    15481 gagaaacaat gagattagca gcaaagcaat tagcttcgct ttcagatttg cttctagatg
    15541 ttcttgttga tacatagtaa ctggataagc cacgatgaaa aatagcgtga cctaattcgt
    15601 gagcgcaaat gtagaatctt tcctcagagt ctctcagttc atcatttaag aagattattg
    15661 cacgacctct aatttcttga aactgtcctt tgggattctc gataaaagga acgtattgaa
    15721 ttttaatgcc catcttttca caaatataaa aaggattagc ggactggtat ttccgcttca
    15781 actcctcgac taaattaatc gtatccatct ccataagctc acatcttttt gcctttttct
    15841 ttgtcttctt tcacaatatc ccagaaggtt ccaataagga tatcttttac gcgttggacc
    15901 tgttcaggcg tcagtgtttc tccgccataa gacatattga catttgaatc tagtaattta
    15961 tcaagttcaa ctacttcatc ttttgttgcc cagtcaggaa cttggtttct accgagtaaa
    16021 taatcagtag ttacattaaa ataatctgct acaagttgta gtcttttagt actaggagtt
    16081 ctatttttcc attgataaat cgtgttttta ggtatattta attcttcttc caattgagtt
    16141 atacttatgc ctcttttatg agctaattcc tttattctat ctagtaaatt cattttcgca
    16201 cctcacaagc tacgaaaaca ctaataaaaa aattagttaa aggtgttgac aactaataaa
    16261 agtgttagta tactgttttc gtaagctaaa atattagcta aaaagacttg ataactaata
    16321 aacacttcac ggtcggcaaa ctctgaaatg taaattacta ggcatttctg tgtctt
//
LOCUS       DARBEC010000060        16356 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.061, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000060 DARBEC010000000
VERSION     DARBEC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16356
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.061"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="SCL80_001903"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="SCL80_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEG1107761.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2944..3378)
                     /locus_tag="SCL80_001904"
     CDS             complement(2944..3378)
                     /locus_tag="SCL80_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107762.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3395..4600)
                     /locus_tag="SCL80_001905"
     CDS             complement(3395..4600)
                     /locus_tag="SCL80_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEG1107763.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5294..5680
                     /locus_tag="SCL80_001906"
     CDS             5294..5680
                     /locus_tag="SCL80_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEG1107764.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5774..5965
                     /locus_tag="SCL80_001907"
     CDS             5774..5965
                     /locus_tag="SCL80_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107765.1"
                     /translation="MNSLKEDFILAKAGNEEAVEAILKRFSSLIHKQSWRTGKYDQDC
                     YQECMLAIYLAISKFEIKE"
     gene            6479..6811
                     /locus_tag="SCL80_001908"
     CDS             6479..6811
                     /locus_tag="SCL80_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1107766.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="SCL80_001909"
     CDS             7107..7262
                     /locus_tag="SCL80_001909"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEG1107767.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="SCL80_001910"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="SCL80_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="SCL80_001911"
     CDS             complement(9119..10735)
                     /locus_tag="SCL80_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1107768.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="SCL80_001912"
     CDS             10853..11071
                     /locus_tag="SCL80_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107769.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="SCL80_001913"
     CDS             11266..11727
                     /locus_tag="SCL80_001913"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1107770.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="SCL80_001914"
     CDS             complement(11920..12159)
                     /locus_tag="SCL80_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1107771.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="SCL80_001915"
     CDS             complement(12183..13706)
                     /locus_tag="SCL80_001915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEG1107772.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="SCL80_001916"
     CDS             complement(13724..13846)
                     /locus_tag="SCL80_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1107773.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="SCL80_001917"
     CDS             complement(13876..14859)
                     /locus_tag="SCL80_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1107774.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="SCL80_001918"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="SCL80_001918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEG1107775.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="SCL80_001919"
     CDS             complement(15053..15430)
                     /locus_tag="SCL80_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107776.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="SCL80_001920"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="SCL80_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1107777.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="SCL80_001921"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="SCL80_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEG1107778.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
BASE COUNT         5186 a         3096 c         2457 g         5617 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa agtacatttc ttcatgtttt tttcggatat ttgtgctttt taaccattca
     2521 gcagctgaaa acatataaaa agatatgtat gtattctttc tgaaattaag ttcatttcta
     2581 tgcgatgaag catgttttca aaatattacc ctttcttaga ttaaacttca gagtattcaa
     2641 ataacggaat aaggagcctt atcccgcatt actgaatgag actgtgacat aagtaaaaag
     2701 gtgacttcaa attccttctt cggaatttga aatcaccttc tttataatta taaagtatcg
     2761 gtttcacagc atttctgaat gattcctaga atcttaaacg aatgattgga gttatcaaca
     2821 ttttttcact cgtttttagc gaaacaaaaa atgacatttg tcacagcctc attaaattat
     2881 tgattctttt tctttataat tgaattgtcc atatttaaag tgtctagtac tctacatgaa
     2941 taattaattg ctttcactca aatacttata atctattaac ttgtagttgt ttaacttttc
     3001 tgaaaaagtt agacccacgt aataatcttc ccagctttct ctttcaatta actgatccaa
     3061 tttttgataa tccataaatt catcatgtat caaaaagaat aaataacttg atattttagc
     3121 atctagctta atcagattaa ataatgtttg tttaaaattg ctaccattaa tatgactgat
     3181 agattctcta gcttcttcac ataaactttt tactttatta attactactt tgtcatattc
     3241 atttaacaca ctattattac cattacttag acgctcagat tctacgaaac aactcaaatc
     3301 agaaatttcc caaaaaaatt ttttgttaaa atccaatttc ctcaactcct ccaaactacg
     3361 taaatgatat agtctcataa taaaataaaa cctctcagct aatgttgttg aaattgtcca
     3421 ctaaacctaa ttgatattgc ttaggagata cacctgcgta ctttttgaat actctcgaaa
     3481 agtgtgaaga atctgaaaat cctaaaccta aagaaacttc actgatagta tggtttgatt
     3541 tcaacattat tttagccgta gaaatttttc gcttagagat ataattataa atcgtaatat
     3601 gcatttcatt cttaaagctt tctcttaatt tagtttcact tacagaaaaa taattagcta
     3661 tttcctttac agtaattctt ttatagatat ttaagccgat gaattgaacg acagaagaaa
     3721 ttaagggaga aagatgctct atcttagttt ttccgatttc cttagtataa taaacaatcc
     3781 cagaatccct cacttttaag acctcaggta acgatacagc taattcgttt tcttgtatca
     3841 aagcatctct actttgatac gcttctatta tatccattcc taatgttata gacgtttgtg
     3901 ctaacttttc aaaaacaatg atagaataat ttttttcaga ccgaatcgta tctccactaa
     3961 cacttggaat tattccatta ctcagattaa aaaccatgtt ttctaaattt cgaatatctc
     4021 catttttaac atattccagt attttatttt cataaagaaa cagatatatt tctggatcgt
     4081 aattttgtga taacagatta tctattttga tttgctcgat agttttactt agattttctg
     4141 tatattcaat taacggttca tggaatagat cttctatttt tccagtaaaa aaataatgaa
     4201 ctaaaatcag aatatctcga atgtctccta aagaatacaa agtcaaattt tgcaatgaat
     4261 tatagagtcg ttctctatca gccattgcaa cattcaaatt gttcattttt agtttaaaaa
     4321 aaactttatc aatattattt gctctaaatg gaccaaataa gtaatatctg gttttaaaca
     4381 tgtatagaag aaacatctca ttaaaatcac cagtaaagaa atggaaatct ttagtttttt
     4441 taatattatc agttagtaac tttttataat cataaaaaaa ataagtagtt ctatctgagc
     4501 gatactcttt tataatactg aattcggaat taaaaaccac gataggtaac tttaaacaaa
     4561 catgaagaga ctttaataag acttctttat ctgaaaacac ttttgtttct ctctttcctt
     4621 tctacatcat taaatagtat tttatacgtt atctaaaata ctattcctct gtcaaaataa
     4681 gctaacttac ttcattataa aatatccact tatcgctttc atgtatattc caccgtaaaa
     4741 agtacatttt atcaccgaga actcatcatt tttcatcatg atgaatacta tttacatata
     4801 tctagttcaa agacaataat tattatgatc cgttattatc taagcactat acagataata
     4861 ggaatttttc gtttctaaat attccaattt gggaatccaa taaaaatttt cgttcaaata
     4921 cgtgtctatt cgaactcttt tctagttgtt aattatgaag ggggaagaaa taaaaaatac
     4981 aatctataca cgaataagaa tattatccaa aaggaggttt tagaaaagat ttattatacg
     5041 taaaaagctc aagacatttt cttagccata aggacatgtt tgatatttaa gaagatatat
     5101 gaattttctt ttctaagtat aaagataact atccattatc aactaaaaaa acatttttga
     5161 aaataaacat cagcagtcct atcaatgcat gtgagcttat agaagttatt cgcagcgcag
     5221 cataattgca ttagcttctt ttcgtgtttt ttgcctcttt tccctttaaa tcttaatgga
     5281 ggaggtgtgc aatatgtcag ataacgacga acgcgtaaat gcccagttga ttcgattctt
     5341 tgaaaaggtc attttgaaca ccgctaatac ttattataaa aaacaagaag aaattcaaaa
     5401 acatgaacaa cttgatcaac tcccaccata tttgtttact aaagatacta taaaatttaa
     5461 acaaccgatt aatgttttaa atgtgactct ccttgttgaa gatataaaat tagcaaaaat
     5521 ccttcctttg ttgaaagaac ctgaacaaat ttttcttatc gaaaaattta tctttgataa
     5581 aactgataaa gaaattggaa aggttctggg gattacaaga cagggtgcga caaatttaaa
     5641 acatcgactt tacaaaaaaa ttcaaaaaat gctggtttaa tatctgcata ataactaatt
     5701 ttgtatgctt ctttgctcgt tctcctcctt cccaaagaaa tagattataa gtccatggga
     5761 agaaggtaga tttatgaatt ctttaaaaga agatttcatt ttagcaaagg ctggaaatga
     5821 agaagctgtt gaggcaattc tcaaaagatt tagctctttg attcataagc aatcctggag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttcgt gtattc
//
LOCUS       DARBEC010000061        16225 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.063, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000061 DARBEC010000000
VERSION     DARBEC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16225)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16225)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16225
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.063"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            138..1475
                     /locus_tag="SCL80_001922"
     CDS             138..1475
                     /locus_tag="SCL80_001922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEG1107779.1"
                     /translation="MSDIIIGILLVISFFFMVWYCVKGYNLMVGFAIMATVWMGLALV
                     GNTFSPNPAMEGQGVIDVLTHIYTTGPAEYAKSILVNVFFGAFFGRVLVDSGIAATLI
                     RKVVELGGDKPRITMSLLCIVTAVIFMSMTGIGPVISIAVIVLPILMSLGISVPVALF
                     SFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTYNDYFQIGMIGMIVSLVVVLTVA
                     NISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVLGVVLLDLPIILGFILAGIWALL
                     FTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMTLAMFNNSAAYASPYFSAIFGDW
                     IPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILAVVLAVNPTMSPAFLFPLFAITT
                     IAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGWIIGAILCLITFLLYGNA"
     gene            1523..3652
                     /locus_tag="SCL80_001923"
     CDS             1523..3652
                     /locus_tag="SCL80_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEG1107780.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLLENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKHLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKSIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPQTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3627..4316
                     /locus_tag="SCL80_001924"
     CDS             3627..4316
                     /locus_tag="SCL80_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107781.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKKIE
                     KRCTEYDLDYK"
     gene            complement(4724..5410)
                     /locus_tag="SCL80_001925"
                     /pseudo
     CDS             complement(4724..5410)
                     /locus_tag="SCL80_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
     gene            complement(5546..5740)
                     /locus_tag="SCL80_001926"
     CDS             complement(5546..5740)
                     /locus_tag="SCL80_001926"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107782.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5790..6230)
                     /locus_tag="SCL80_001927"
     CDS             complement(5790..6230)
                     /locus_tag="SCL80_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEG1107783.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELDQLFQAYASLDDVNS"
     gene            complement(6234..7079)
                     /locus_tag="SCL80_001928"
     CDS             complement(6234..7079)
                     /locus_tag="SCL80_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEG1107784.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVVWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLAANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAIYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            7228..8646
                     /gene="pepV"
                     /locus_tag="SCL80_001929"
     CDS             7228..8646
                     /gene="pepV"
                     /locus_tag="SCL80_001929"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEG1107785.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
     gene            complement(8703..9650)
                     /locus_tag="SCL80_001930"
     CDS             complement(8703..9650)
                     /locus_tag="SCL80_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEG1107786.1"
                     /translation="MEQQEKIAVLIPCYNEEATISTVIADFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAVHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVRNTITRMYKTKILDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMFKDYKPLLFFGIWTIFFFLFGLVTGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYHSRK"
     gene            complement(9873..10223)
                     /locus_tag="SCL80_001931"
     CDS             complement(9873..10223)
                     /locus_tag="SCL80_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEG1107787.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKSSFLKEGAIEGSWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLEISRKKGATD"
     gene            10423..11502
                     /gene="pepA"
                     /locus_tag="SCL80_001932"
     CDS             10423..11502
                     /gene="pepA"
                     /locus_tag="SCL80_001932"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEG1107788.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            11646..11966
                     /locus_tag="SCL80_001933"
     CDS             11646..11966
                     /locus_tag="SCL80_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1107789.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VTKG"
     gene            11988..12452
                     /locus_tag="SCL80_001934"
     CDS             11988..12452
                     /locus_tag="SCL80_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1107790.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKAVEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKEMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYEIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            12657..13262
                     /locus_tag="SCL80_001935"
     CDS             12657..13262
                     /locus_tag="SCL80_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEG1107791.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVDSYGMICSARELHLPNAPAKKGILVLPEDAVVGEAFPK"
     gene            complement(13301..14590)
                     /locus_tag="SCL80_001936"
     CDS             complement(13301..14590)
                     /locus_tag="SCL80_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEG1107792.1"
                     /translation="MDRKNVTPKMKKSKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVLMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            15018..15497
                     /gene="rlmH"
                     /locus_tag="SCL80_001937"
     CDS             15018..15497
                     /gene="rlmH"
                     /locus_tag="SCL80_001937"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEG1107793.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYALAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            15700..16225
                     /locus_tag="SCL80_001938"
                     /pseudo
     CDS             15700..16225
                     /locus_tag="SCL80_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336126.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
BASE COUNT         5134 a         2971 c         3297 g         4823 t
ORIGIN      
        1 cataattaag gttttttaat tgaaaactga acatttatgg tcacccttta atgaaagcgc
       61 taactacata agttagaatg agtttgactg atatattagt ttgcttatca gaaaaacaaa
      121 atggacggag gaataacatg agtgatatta ttattggtat tttgttagtt atctcattct
      181 ttttcatggt ctggtactgt gtaaaaggct acaacttgat ggtcggcttt gccatcatgg
      241 ctactgtatg gatgggatta gcgctagtcg gaaacacgtt ttcaccaaac ccggcgatgg
      301 aagggcaagg agtaattgat gtgttgacgc atatctatac gacggggcca gctgagtacg
      361 ccaaatcgat cttggtaaat gtcttctttg gtgccttttt cgggcgtgtt ctagtagata
      421 gcggaatcgc agctaccctg attcgaaagg tagttgaact tggaggggac aagccaagaa
      481 tcacgatgag cttactatgt attgtaaccg ctgttatatt catgtcgatg acaggaatcg
      541 gaccagtgat ttctattgca gttattgtgt tgccaatctt gatgtcatta ggtatttcag
      601 taccggttgc actgttctca tttatgggct cgattatggc tggaatcttt gctaatatcg
      661 tcaatttcaa acaatatcaa acgatttatg ctggtttcaa tccagcggct gaaagctata
      721 cttataatga ctatttccaa attggtatga ttgggatgat tgtaagctta gttgtggtac
      781 taacggtcgc taatatatca atgaacaaaa agaaacgcta tgcaatggca gcaaacgtac
      841 cagctgaagg tggcgacgca ccaatgatct catggttagc cgtattatta ccagttttag
      901 gggtcgttct tttagatctt ccaatcatct taggttttat tctagcaggt atctgggcat
      961 tgctgtttac tggaaaattg cgcggaggtt ataaagaaat ctgtcgtcag tttgcgaaat
     1021 tatttacaga tggcgcagta gatgttgcgc caatggttgg tttcttgatg accttagcga
     1081 tgttcaataa ttcagctgcg tatgcttctc cttatttttc agctattttt ggagattgga
     1141 taccgcaatc accattagtc ttggcaatcg tatttgctat attgacacca ctaggatttt
     1201 tccgcggtcc aatgaatcta gtcggttcag gctcagctat tttagctgtc gtattagctg
     1261 ttaatccaac gatgtcgcca gcctttttgt tcccgttatt tgcaatcaca acgattgctc
     1321 ctcagcattt agatattaca caatcttggg ttgcttgggg attaggttat acaaaagtaa
     1381 cttctcgaga atatatgaag aaatccatcc caactggttg gatcatcgga gcgatcttat
     1441 gcctgatcac gttcttattg tacgggaacg cctagtaaac aaaacgtcga taaaaacgat
     1501 tagatgaatg gaggaaaaga aaatgaaacg ttttgtgcga atgggaattg atgttggcgg
     1561 tacacatacg aaagcagtcg caatcgataa tgcgacgcac gaaatcatcg gaaaatcttc
     1621 agtgaaaaca acccacgatg atgtccgtgg agtagccgca ggtgtcgttc agtcttttca
     1681 aaattgtttg ctggaaaata atatcagtcc agaagatgtt gtgtttgttg cacattctac
     1741 cacacaagcg actaatgcac tgattgaagg agacgtagca aaagtcggcg taatcgggat
     1801 ggcaaaaggt ggattagaag gttttttagc aaaacgacaa actagattga acgatatcga
     1861 tttagggaat aagaaaaaaa tagagattgt caatgcattc ttacctgtca aacacttaaa
     1921 tgtagaccgt gtttcagaaa cgatttcttc tttagaaaga gaaagagcgg aggtcttggt
     1981 ttcttctatg gcatttggag tagataatgg ggaaccagaa cgagttgttt atgaagcagc
     2041 atcggtaaaa tcgatcccta caacgatggc ttctgacatc acaaaattat atggattgac
     2101 aagaagaact cggacagcag caatcaatgc atctatttta ccaaaaatgc tagatacggc
     2161 aacctctaca gaagattccg tacgagaagc aggggtcaat gtttctttga tgattatgcg
     2221 cggagacggc ggagtaatgg aaatcaatga aatgaaaaaa cgacctgttt taaccatgct
     2281 ttccggtcca gcagcatcag tcatgggctc tttgatgtac ttgagagcgt ctaatggtgt
     2341 ttattttgaa gtaggcggaa caacaaccaa tatcggggtc attaaaaatg gacgtccagc
     2401 aatcgattat tcaattgtag gtggccaccc aacatatatc tcttcattag atgttcgggt
     2461 tctaggtgtg gcaggcggtt cgatggtacg ggctaatcag tcaggaatca tcgatgtcgg
     2521 tccacgttct gcacatattg ccggtctaga ttatgcagta tttacggaga cagaaaaaat
     2581 caaaggtcct aaggttgaat ttttctctcc taaagaagga gaccctgctg attacgtcaa
     2641 ggtagtgatg gaagacggag aagaagtgac tattacaaat acatgtgcag ccaatgtcct
     2701 tggattagtg caagaagaac atttttctta tggaaatgtc ccatctgcac gaaaagccat
     2761 ccaagcatta gctgattatt gtcatacgac ggttgaagac atcgcagaac agatcatgga
     2821 aaaatcctat gctaagattg aaccggtgat tttagaattg gcagacaagt atcacttgga
     2881 gaaagatcag atttctcttg taggtgtagg gggcggcgct gcctcattga ttacttactt
     2941 tagtaataaa atgggtgtga aatattcgat tcctgaaaac gccgaagtga tttcttctat
     3001 tggtgtagca cttgcaatgg tccgagatgt tgtagaacgt atcatccctt cgccaagtaa
     3061 agaagatata cgatcgttga aaaatgaagc gatgaataaa gcgattgaat caggcgctac
     3121 accagaatcg atcgaagtcc acgtagaaat cgatccacag acttcaaaag taacggctat
     3181 cgctactggt tctacggaag tgaaagcaac cgatctaacc aaggagatca caacagaaga
     3241 agcactagag cttgctgcag aggatatgcg attaaataaa aatgaagttt gcttattgga
     3301 aaatacacct ttcttttatg tatgcggcga acagaatcgt tcaaaaaatg ctggaagtct
     3361 tcggatcatc gatcaaaaag gatttatcaa ggtccaacgt ggacatgctt catgtatgaa
     3421 aacaactgca gccaattata tgacagctgt cgaacaactt tgggaagata tggcagttta
     3481 tcaaactgaa ttgattgccc gaccagaatt ttatctctgc ttaggagcaa gagtctctga
     3541 ttttacagcg acggatctgg aacaactaca gcttttgatg gatctggaag tatctactat
     3601 ggaaccggag gaagaagtga tcgtcgttgc cggaaatatc aaacaaacat aagttagaag
     3661 agttactaaa cgaattacaa cagatccccg aagaaatttg gggattctac cagtttcggc
     3721 gagatttatt ttggaaaaag attcctttgt ccaagcagaa aattcttata caacaatcaa
     3781 tcgattgtgg tatcgaaacg gcatgtagca tcaaaaaaaa gtaccctttt gccgatgtag
     3841 gagaaatttg tgaacaaatg gctgttccaa tcgtttcctg tgaatcagag caaataaacg
     3901 aacgaatcac ttttgcaact tatgcagaag atgaagggat tcgattgatg acggagccgt
     3961 tagaaaaatt aaaatgttca ggactagctt cgatctctaa agaaacagcg caagctttga
     4021 ttattggaca tgaactattt caccacatcg aagcttctgt taagggaatt tatacgcaaa
     4081 gcgaaaaaat tgtgctgtgg cggttgcctt tttatacaca tcaatcaaac attcgtgcat
     4141 tgagcgagat agcagcgatg agtttttcaa aagagatgaa tcaaagcgat ttttcgcctt
     4201 atgtattaga ggcagtctta ctttggccat ataatgaaat tcgtagccaa gggatcttag
     4261 aagaaataaa aaaaatagaa aaaagatgta cggagtatga tttagattac aaataaagag
     4321 actgtgacaa aagtcgtgtc acagtccttt ttttccgaat aaacggtgtt ctcctgtcaa
     4381 ctcctcggtc acaagtcaaa atattcaaca aacatgagaa gctgtttttt gaaattgttc
     4441 cttgtgactc ggagatgaac ggcaggctca cgacctcttg gaaaacggtg ttcaaaagct
     4501 tgcatctgcg gtaataagtc cagccaagtt aaaaacataa gaagctattt ttacttggct
     4561 gttcttatta cttgatgcag ggcagctttt tcacaacctc tcaataaacg gtgtgcaatc
     4621 agctgaccct cggtattagc tcaaaaaaaa cggcaaaata tgaggaacta ttttgccgtt
     4681 tctttctcta atactcaggt caaaacgctg attccaaaac ctcttatttg atgggtctgt
     4741 cctgaatatc ttcaaaataa tcggggtaat tatgaatcac gtattcttgc tcctcgataa
     4801 tcatatgtaa atgcatcgtg caaagaaatc cgacttcatc aaagttatga tcaatcattg
     4861 attgaagcat tgtctgatgt tcatctaata ctcgacccca gtctaattct gaaatcgtca
     4921 aacgtaacca tctgaaacgg ttcaagtgcg tactataact ttctagccaa tcccaaattt
     4981 ctcgttttcc agcaatttca taacaagttc ggtgaaaatc agtatccaaa tcaaaaaaag
     5041 cttttttatt ttgagcgatt actgcctgat cggtttgttc tagtattttt tctagtttag
     5101 cttgatctta cttggtcatt ttcgcactgc attcttgcag gatcggtttt tccaactttt
     5161 cccgcgtata acgggctaat gaagcagatc ggatatcgat ttttgaaaca taagtgccgg
     5221 actgtggaat ggtttttaca agctcttgat tgcgcagttg gatcagtgct tctcgaatcg
     5281 gtgtacgccc gatagctagt tgttcttcta acactttatc ggagatttta cttccaggtg
     5341 ctaactcgga ataaatgatt tgtttacgta tagattcata agcttgctgt tgcaaattat
     5401 tgataaccat ttaaccttcc tttctatgca gatataatct attataacat aaatcgctta
     5461 caaatcgtgt gggcataaaa acacagtctg caaaaaaagc cctgataggc atgcctatta
     5521 gagcctcgaa aagaaaaata ttgaatcaat ctggtcgtac ctcttggatc ggtttgacac
     5581 gaccgccgat tcttttagca aaagcttcgg attctttttt gtctttgaaa atgagcagat
     5641 tgtctaagtt tttttcccga gtgcgacagc cagttttatc gatgtaaccg tgcattactt
     5701 taacgacata catggggatc acctgctttt ttcttttcat tatacgaact actcgacaca
     5761 atacaagaaa aatgcttaga cggtctttat taagaattta catcatccaa agaagcgtac
     5821 gcttggaata actgatctaa ctccggattg actttgctca gcttcactaa tcgttgcgaa
     5881 tcagaaagta aaaaacagtg tttactactg ccagtagttt ttaatttttt gtcttcagtt
     5941 cccacaattt cgtagctaaa atccaaccgt gtccccgtat acttttggat agttggatgg
     6001 atgctcactt tttctccgta atggatcatt tctttatagc tgcaatttac ttccagaacg
     6061 gggatgatga tcccagattc ttcgattttt ttatagggaa accctagatg taccaataag
     6121 gctacccgag cctcttcaaa ccagcggata taattgctgt gatgaataat ccccatttga
     6181 tctgtttcat aataaaaagg ttctcgcaca taacctgtaa atttctccat gactcatgcc
     6241 tcgttttcca ttgatttttt acaaaaataa ttcatccatt tcggaagtgc ttcgctgatt
     6301 tgtgtcggca aaacaatata ttgttctttt gccagctggt ctccaatgaa actatgaaga
     6361 taaatagctg ctgcaatcgt ttggtcattt tgaggaaact gggctaaaaa tcctgtgatc
     6421 atcccagcta aagtgtcacc catgccgccc gttgccatag cagcagaacc taaaggattg
     6481 gtgtagaagt gctggtgacc ataaattttt gtctcatgac ttttcaaaac gagcacagca
     6541 ccgatttcct gttgcttctt tttgttttct ttatcccgtt gttcttgtat cggtagagaa
     6601 ctcaaccgtt gccactccat ttgatgaggt gtaagtacag tttgttcggg atagggtagc
     6661 aaaagatgat tagcagcaag taaacttaaa gcagagccat caatcaccag ccattgttct
     6721 tttgattggt tttctagtac ttgtttgaaa cgttccaaac tcttagaaga agtacccaat
     6781 ccgggaccaa tcaagagtac atctgcttgc tttattacgg aaagaaactg gtcagtatcc
     6841 gtccaaacta caatcattgc ttctggcaga tgactgtgaa gagcactccg atttttttca
     6901 tcagttacta cagtcgtgag acccgctccg ctgtgtacgg cagctaaagc actcataatg
     6961 attgctccgc catattgttc attgccgcca atcaaaacga tacgtccaaa tgttccttta
     7021 tgtgaaattt ttggacgagg tgtgatgact cttttcacta gttgttcgtt tatcagcatc
     7081 taaaaacctc cttttactca ttatagcgta tatttttttt agagaaaaac agataagaaa
     7141 aggaattgtc agattttttg aactattttt ttctgtaatt tgtggtatca tgaacacgaa
     7201 gacttaaata agtggaggat taactttatg acaattgatt ggcaaaaaga agtagaagca
     7261 cgtaaagaag acttgcttga agacttgaaa aatcttttac gtgtcaacag cgagcgcgat
     7321 gactcaaaag taacaccaga tgcaccattt ggacctggac ctcgcgacgc attgaaacat
     7381 atgttagctt atggcgaacg tgatggattt aaagtaaaaa atgtcgataa ctatgcaggg
     7441 catattgatc ttggcgaagg agacgaaacg ctagggatat ttggacacat ggatgtcgtt
     7501 cctgcaggag atggctggga cacagatcct tatgaaccag taatcaagga tggaaagatc
     7561 tttgcacgcg gatcaagcga tgacaaaggt ccaagtatgg cagcttacta tgcgatgaag
     7621 atcattaaag aacttgactt gaaactatca aaaaaagttc gcttcgtagt cggcagcgat
     7681 gaagaaagcg gctggggcga tatggcttat tatttcgaac atgaagaaga accagatttt
     7741 ggtttctcac cagatgctga attcccaatc atcaatggtg aaaaaggaaa tgtttcttta
     7801 gctttacgct tcaaaggcga caatgcagga gattatgtct tgaaatcttt tgtttccgga
     7861 cttcgcgaaa atatggtgcc aggaacagca actgcagcac ttcaagtacc aagtgcagat
     7921 gcagctatcg cgatggaaga agcattttac caattcatcg aagcaaatcc agtaagcgga
     7981 acgattgaag cggacaatac gtatgtaaaa atcgaattag ttggtaaagg cgcacatggt
     8041 gcaagcccac aatctggtat caatgcaggt tctttcttgg cattgttctt agataactat
     8101 gaattcctag gatctgctaa acaatttatc catgtagcag ctgcttatgt ccatgaagat
     8161 ttttatggag aaaaactagg agtcgcttat gaagatgaaa aaatgggcaa attgacaatg
     8221 aatgctggac tatttgcttt tgaagaaaat ggtacagagg aagcgaactt catcaatatg
     8281 aatttccgtt ttccaaaagg tgtaacagta gacggattgc aaagtgacat cgaacaaaca
     8341 gtggggcaag aaggcgcaac agttactcga ggtgctcgcg taatggaacc acattacgta
     8401 ccaatggatg atccattggt ggctacatta ttgcaagtct atgaagacca cactggagaa
     8461 aaaggctacg aacaaatcat cggtggcgga acttacggac gtttgttgaa acgtggcgtt
     8521 gcttatggag cgatgttccc aggatatact gatacaatgc atcaagcaaa cgaatttatg
     8581 agtcttgatg acttgttcag agcaacagca atctatgcag atgccatcta tcgtttggcg
     8641 aaataattaa ttaaaaagag gatcaagaat tgcaaattat ttttgcagtc ttgatccttt
     8701 ttttactttc tactgtggta ttcatgataa acatgatata gattcaattc atattctttc
     8761 ttagcgttcg tgacgactgt atccaaaatc aaccctgtta ctaatgaaag aagtgctaaa
     8821 gtcatcaatc cagtacttaa aatcgcagaa gggatgcgtg taataaagct tgtcagcata
     8881 tattcgcgaa tcacaggaat ccccgtgacc aatccaaata aaaagaagaa gatggtccaa
     8941 atcccaaaga aaagcaaagg cttataatct ttaaacattt taataatcat catgatcact
     9001 ttgaatccgt cagagaatgt attcaatttt gattcgctgc cttcaggacg atcacgataa
     9061 tcgattggga tttcaacgaa cttgaatttt ttatctaaag catggatcgt caattccgtt
     9121 tcgatttgaa aaccagaact cataatagga aaacttttga caaagatacg attgaatcct
     9181 cgatagccgg tcatgacatc aagaattttt gttttataca ttctagtaat tgtatttcta
     9241 acaaggttat ttccaaaatc gtggaaaggt ctcttattct catcaaagta ggttccattt
     9301 gataggcgat caccaatgac catatctgct tcaccagaac gtaatttctc gagcaaacca
     9361 tgtacagctt ctgcaggata agtatcatcg ccatctacca ttaaataata gtcggcatcg
     9421 atatcaaaga acatttggcg gatcacattt ccttttcctt gacgaggttc tttttttacg
     9481 attgctccac cttgaacggc caattcgtat gttcgatcgg tcgaattatt atcatagaca
     9541 taaatatctg cttctggtaa ttctcgttta aagtctgcaa tgactgttga aattgttgct
     9601 tcctcattgt aacaaggaat cagaacagca attttttctt gttgttccat ttttcactct
     9661 ccttaactat agaaaacaaa agctaaatta aaaatacctc ataatcaaag gaaatgctag
     9721 tttttcccgc taaatatcat ttatttaaaa agaaaatact ggaatatatg tttggaaagc
     9781 tctatctaaa aaaccaccca ctacaaaaga aggacatgat acctctcttt tgaatagtgg
     9841 gtgcgtattc gttctctcac ttttttgacg tattaatctg ttgcaccttt ttttcttgaa
     9901 atttctaata cggttcctgt atgagcatct gctaaaaatt cataataaac agtttcgcca
     9961 tcttctattc gagaaagacc tccccgataa gcactcgacc gaacggcgaa tttcctaata
    10021 ggttcctttt caaaagagat ccaagaacct tcgattgcac cttcttttag aaaagatgac
    10081 ttgatatttt caagtacttg gtctgctgac aatacttttt tgcgatgata ccaaatcgtt
    10141 gaagctaccc cgctcaataa tcctagaccg attccaactg ctaacccgcc tttaaaatag
    10201 cttagttcgc tttcttcata catgtggaca tacctccttc ttctcacttg ttactccttg
    10261 tgcttatttt accacactct catattttct ttgaaataaa taaccgctac cagaaaaaat
    10321 aagctataat aaatacgatc ggtctgcaga aaaaatcata ttttatccgc agatcaatat
    10381 aagtctgaga ggagtttagt ctgaaattta gacgaatagc atatggaaga aaaaacattt
    10441 caacgaatca aagagttaac agaattacaa ggaacaagcg gttttgaaca agatgttcgc
    10501 gcttatatgg aaagtcatat ggaaccgctg gtagatgagt tacagttaga tggtttgggc
    10561 ggcatttttg gtctgcgcca tcatgaagaa gcggatgcac cccgtgtaat ggtcgcagca
    10621 catatggatg aagtcggatt catgctgaca caaatccaag aaaacgggct ttttaaagtc
    10681 gtacctctag gcggctggaa tccttacgtt gtttctgctc agcgttttac acttaaaacc
    10741 tctactggta agaattatcc atgtatttct tcttccgttc ccccacattt attgcgagga
    10801 acaagcggac aaaaatcagt agaagtaacc gatattttgt tcgatgctgg gtttgaatca
    10861 agagaagagg caatgagtta cggtgtcctt ccaggagaca caatcgttcc ttacgctgaa
    10921 acgatcaaaa cagccaacgg aaagaatatt atcagtaaat catgggacaa ccgctatggc
    10981 tgtacaatgg tattagaagc tttagaagcg ttgcaaaatg aaacattagg tcatacattg
    11041 attgcaggcg cgaatgtcca ggaagaagta ggcttgcgtg gttctaaagc ttctgtcaat
    11101 aaattcaagc cagatctgtt ctttgcagta gactgctctg cagcagatga tacagtgacg
    11161 aaaaatggca cattcggaca tttaggagaa ggtacattga tgcgtatcca agatccaggt
    11221 ttgatcatgc ttcctcgttt gcgcgagtac ttgctggata tcgctgaaac aaacaatatc
    11281 ccttatcaat actttgtttc aaaaggcggt acagatgcag gtgctgctca tacacaaaac
    11341 gaaggaatcc caagtacagt gattggcgta gtgggacgtt atatccatac gcatcaaacg
    11401 atgttcagta ttcgcgattt tgaagctgca cgagaaatgt tgattcaaac actgaaaggt
    11461 ttggataaat caacagtaaa tacgatcgta tacggaaaat aatgcgatag tgaaactata
    11521 aaatctggca aaagattgct ttttgccaga ttttttttat aaaaagagga tcaatgtttg
    11581 ttagttcgaa agaaaggtgt ataatttaat tgaaaaatac ataaacagaa agtagaggac
    11641 aaagaatgat tatcccaaat agtttagaag aattagcagg ctacgtagaa actggaaaga
    11701 gtgtattttt ctttactgcc ggctggtgcg gagactgccg ttttatcaaa ccgcaaatgc
    11761 ctgaaattga aaatgacttt tctgattggc aatttattga agtagaccgt gacaagtaca
    11821 tagatgtagc cgctgaatgg aacatttttg gtatcccaag ttttgtcgtg atccaagacg
    11881 gaaaagaatt aggtcgtcta gtgaataaag atcgtaaaac aaaacaggaa atcgaatcgt
    11941 tcctagaaag tgtaacaaaa ggctgacatc tgttaggagg ggtcaagatg aatcaagaat
    12001 ataagaatat tttagtaggt atcgacggta gtgaacaagc acttgctgct ttcaaaaaag
    12061 cggtagaagt ggctcgccgt aataatggga aagtatatgt agcaaacgta atcgatcagc
    12121 aattttataa ctttatgggc tatgcaccta ttgatcaaaa tctagtcgat cagcaaactg
    12181 aaacagccaa ggaaatgatc gaagaatgta aagaatatgg gaaatctgtt gattacacaa
    12241 atatagaagg aattattgca tatggttctg ttcgtgaatc catggcagtc aaattgccaa
    12301 aaaaatatga gatcgatcta atcatggttg ggcagtcagg tctaaatgca gtcgaacgct
    12361 ttatgacagg aagtgtagcc agctacgtga tacgtcattc accttgtgat gtcttgatcg
    12421 tttctgacga agaagaacag ccaagtaatt aagcaagtgg aacttgtacg acctttttaa
    12481 tcaattaact aaaacagtaa agggactgtg acaaaagtct tgtcacagtc ccttgttacg
    12541 aataaatggt gttccagaag aggcttcttc ggaaataaag catgaaaaaa atttcatgca
    12601 gtgaatgcaa ttttttgtta agataaatct cagcaaagga taatggagga aagaacatga
    12661 tttttgctta taacaaagca cacgtcggag acaccttgat ggttattata gccgacgata
    12721 aaggaacaga aaacactgtt gaacgcaaat ggaatgttgc tcggatcaaa gatgaacaag
    12781 gtcaagttgt tgcttggaac tttttccata tctccgatca tctaacaatc gaagggaacg
    12841 gccaagtaag cttaacagat gaggaattag cgaaattgaa tcgattgatc aaagaagcag
    12901 gattcgaaga acagcttgaa gctgatcatg aaccgaagat cgttgtcggg tacgtaaaaa
    12961 catgcgtccc acatcctgat tctgaccatt tatcgattac tgagacagaa gtagacaatg
    13021 gtacagtatt gcaaatcgtt tgcggtgcac ccaatatcga agctggacaa aaagttgtcg
    13081 ttgcaaaacc aggagctatg atgccagatg gaatgatgat ctggccagga actttgcgag
    13141 gggtagatag ctatggaatg atctgttccg ctcgtgaatt gcatttgcca aatgctccag
    13201 caaaaaaagg aatcttagta ttgcctgagg atgccgtagt cggagaagca tttccaaaat
    13261 aaagaggttg tgacaagtct ttgtcacaac ctctttttta ttaattttca ctattttgat
    13321 tttgttttaa tgccgattga tcgatcgtca attcaacagt agttgttttt tctttcgagc
    13381 cttcatagta agtgattttg acagaatctc ccactttttt cttatacaaa gcagattgaa
    13441 gttctacacc agaagaaact tctttgtcat cgatcttagt aatgacgtcg tattgtttca
    13501 atccagcttt ttcagccggt gtagctgttt ggacatttgt cacgatgaca ccgttagtta
    13561 cagatgaagg aatcttcaag atttgttctt gttgctgagt agaaacggca gataagtcga
    13621 ccattgtgat acctagagct ggacgtgtca cttttccgtc tttttccagt tgattgatga
    13681 tattcactac atcgttactt ggaatagcaa atcccattcc ttctacgctt acattagaag
    13741 tagattcaga agtacttgcg atcttacttg agttgatacc gataacttgt ccttcgatat
    13801 taactaaagg accaccagaa tttccagggt tgattgctgc atctgtttgg atcgcattga
    13861 tattgactgt ttcgttcgat tcattcgtac ttgtcacttg tcggttcaag gaagaaatga
    13921 ttcctgaagt gacagaattt gcatattcag atcctagtgg ggaaccaatc gcaatcgcag
    13981 gctcaccaac cttcagagca cttgagtcac caaaagaggc aactgtttcg actttgtctg
    14041 aattgatctt caacaccgct aaatctgaat aagcatctgt tccaactaat tcagctttta
    14101 ccttcgtacc gtctttcatc aagacttcaa gaccttgctg gccatctact acgtgattat
    14161 tcgtgactac ataagcagta ttcccgtctt ttttgtagat cacgccgctt ccttcactat
    14221 aagcttccaa attactatca tctgagctgc tactttcttc ttgctgcccg aatagtccgc
    14281 caaaaccgct tgattggttt tgactttgta agttgataac agaaacgaca gcgtcttgaa
    14341 ctttatctac cgctttggtg atatcagaat ctacattgac tttgacattt tccacaactg
    14401 tttcaccggc agaattctga ttgccgcttg tagcagtaga actattgttg ccagtgccca
    14461 tcgctgcata aaaaatgcca gctgtcagta gtcctccaac gatccctcca acaagcccca
    14521 gacctaactt acgccagagg ctattatttt tacttttttt catctttggt gtcacatttt
    14581 ttctatccat gtgttcctcc tccaattaca ttcatttatc tactgttagt atagtcttat
    14641 ttgttgactt tagtctactt tgagactgtg aactttttat gaaaaacttt aggaaaaatc
    14701 attgttttca gatgaaaata tttgattttc tttttccaca gatactgtgt aaaactacgc
    14761 ggataggatt ggaaaaataa gggataaaac atatccacag cctgtgtata aagtggataa
    14821 attcttataa aacgtatgtt tgcctaatga atgtgatgaa ataaggtaaa ataacactgt
    14881 tttctgtgga taaacctgtg gatgttgtgc ataactgttg gggaaatctt tttaacaaaa
    14941 atagccaaaa taaacatata gacgaaaaaa ttgattataa taaacaaagt gagcatgaag
    15001 accaaaggag agaaaacatg aatatcaaaa ttatctcagt aggaaaatta aaagaaaaat
    15061 acctgatcca aggtatcaat gagtatgtca aacggctgaa tgcctatgca aaaatcgaat
    15121 tgatcgaagt tcctgacgaa aaagccccgg agaatttaag cgaagcgcaa atgagacaag
    15181 taaaagaaaa agaaggggaa cggatcttag cgaaaatcaa agaacaagaa tacgtctatg
    15241 ccttagcaat tgaagggaaa aatcctacaa gtgaatcttt tgctaaacaa atcgaccaat
    15301 taggtatcca aggaaaaagc caccttgttt ttgttatcgg cggctcttta ggactcagcg
    15361 aagccgtcat gaaaagaagc aatgctcaga tctcttttgg aaaaatgacg tatccacatc
    15421 aattgatgcg tctgatcctt gtcgagcaaa tctaccgtgc cttccggatc aatgcggggg
    15481 ctccatacca caaataatga tattttttaa gagaaaaaat tatccctatt ttaaataata
    15541 gcaataggta gtggtaggga gaaatttata aataactttt ttatttgctt tggtagccaa
    15601 gtaagttgca aaagaaaaat agatactgtt aaaaagataa tgtagacata gaaacaaata
    15661 cgtaaaattt tagttagaat aatggcaagg agaaaaaaca tgatagatga aaaaaagtgt
    15721 ttaaaaaatc tattagagga tatggatgat attgaagaaa actatctaaa tggactggga
    15781 aaacaaatat atttcagata cttaagctat ctaaaaatga aataagacat agtaattttt
    15841 tagctttttt gtttgctcca aatgaaaccc acaatttatc tgatgagttt tttaaaatgt
    15901 ttttgaaaag gtatatagat tctaatgatg ataccaaagc tgctatcaat tattttgatg
    15961 ctttacttaa tagctatgaa gatttaattg tttacagaga aaataagaat attgatattt
    16021 tattggtatc ggaaaaaaat aaaatagtgg tgtgcatcga aaataaaatt ctgtcttcgg
    16081 agtctggagg gcaacttaat aaataccaaa tatatataga acgcaattat tctaactata
    16141 aaaaaatttt tgttttttta actccagatg gaaatgaacc cacaaatcct agttggggca
    16201 taattactta caagaatatc gttga
//
LOCUS       DARBEC010000062        16219 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.064, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000062 DARBEC010000000
VERSION     DARBEC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16219)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16219)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16219
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.064"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            226..894
                     /locus_tag="SCL80_001939"
     CDS             226..894
                     /locus_tag="SCL80_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEG1107794.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFSLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
     gene            complement(1094..1480)
                     /locus_tag="SCL80_001940"
     CDS             complement(1094..1480)
                     /locus_tag="SCL80_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107795.1"
                     /translation="MRRTAKKFIDYNEYRDRPFGLKWGTAFAMEELVNGIKKNEEEAR
                     KQPLPKQQMTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGEAYEDYF
                     MIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(1452..2834)
                     /locus_tag="SCL80_001941"
     CDS             complement(1452..2834)
                     /locus_tag="SCL80_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEG1107796.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            complement(2990..4177)
                     /locus_tag="SCL80_001942"
     CDS             complement(2990..4177)
                     /locus_tag="SCL80_001942"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEG1107797.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            complement(4184..5215)
                     /locus_tag="SCL80_001943"
     CDS             complement(4184..5215)
                     /locus_tag="SCL80_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEG1107798.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVGIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            complement(5231..6685)
                     /locus_tag="SCL80_001944"
     CDS             complement(5231..6685)
                     /locus_tag="SCL80_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107799.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANVATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEELENKLAPADTMEDELHDAVVPSH"
     gene            6860..7765
                     /locus_tag="SCL80_001945"
     CDS             6860..7765
                     /locus_tag="SCL80_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEG1107800.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            7856..8533
                     /locus_tag="SCL80_001946"
     CDS             7856..8533
                     /locus_tag="SCL80_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1107801.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            8530..8700
                     /locus_tag="SCL80_001947"
     CDS             8530..8700
                     /locus_tag="SCL80_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107802.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            8814..9710
                     /locus_tag="SCL80_001948"
     CDS             8814..9710
                     /locus_tag="SCL80_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1107803.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(9765..11135)
                     /locus_tag="SCL80_001949"
     CDS             complement(9765..11135)
                     /locus_tag="SCL80_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1107804.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            11373..12119
                     /locus_tag="SCL80_001950"
     CDS             11373..12119
                     /locus_tag="SCL80_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEG1107805.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQPVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            12116..12955
                     /locus_tag="SCL80_001951"
     CDS             12116..12955
                     /locus_tag="SCL80_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEG1107806.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            12956..13906
                     /locus_tag="SCL80_001952"
     CDS             12956..13906
                     /locus_tag="SCL80_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEG1107807.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            14047..14799
                     /locus_tag="SCL80_001953"
     CDS             14047..14799
                     /locus_tag="SCL80_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107808.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            14818..15969
                     /gene="queG"
                     /locus_tag="SCL80_001954"
     CDS             14818..15969
                     /gene="queG"
                     /locus_tag="SCL80_001954"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEG1107809.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEVYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
BASE COUNT         5267 a         3023 c         3250 g         4679 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacggt tgtgtaaaaa tatcatttta gagcataaaa
       61 aaaaggagtg gatttctcca ctccaaaaaa gtctaacttt tgggggtcac tacagtgtca
      121 cagtcctttt tttagtaaaa ggatacattt cttccttgta tattccatgc gtgttcgtga
      181 gaaagcatat ataatttaat taacagatag ttagggggag aaaaaatgat tttttcaatt
      241 acaatgaaac tggtgttcgg gttgatcggt ttattattag tggtacgttt gcttgggaag
      301 aaatccatgt cagaaatcac accgtttgat ttagtctata cgcttgtttt aggtgggatc
      361 ttagaagaat caacctatga tgacaatgtc catgtagggc atgtcctatt tgccattgcg
      421 ctttgggcag taatgattta tggtatcgaa cgtatcgtcc aaaaaaatga aaaggtcaat
      481 cgttgggtca aaggcgagcc atctgttcta atcaaagacg gcgtcatcaa catgacagag
      541 ttgacaaata accatatcga gatggagcag ttgcgagcaa tcttaagaca gcaagaatgc
      601 ttttctcttg aaaatgccaa acatgttatc ctagagaatg ctggtcaaat gagtgtattg
      661 aaaaaatcag atgaagacaa agctttatcg atcctgcttg tagatgaagg gcaaattcag
      721 cataaagtgc ttcagtccaa tcaattgact gaagcatggc ttatggagaa cttgaagaaa
      781 gaaggctatg cagatgtaaa acagctgatt tatgttgaat ggtcagaaga aaaagggctc
      841 tacatactaa ataaagaaga cgtcatgcat gaatcttacc gaatagatgg atagatagac
      901 agaggttgtg aaatcagcgc cttggcttgt tactcaaaac taagcgactt tttcacaacc
      961 tctgatatct aatatacgtt gttccagaag ttgcttcttc gaaaaataag ccaaaattcc
     1021 acaaaaattt aagaagcaat tttcgaaaaa tcctttctta ttttttgaag ttgatcactt
     1081 ctgtcacaac ctctcatgtc acaaaccatt tttttgtttc ctttaactca atataccgta
     1141 cgtcttccca caatatcggc tgatggtcaa tcataaagta atcttcatag gcttcaccaa
     1201 tgaagtgccc ttcgacctca tccaagagtc ttccaaattc atcttttaaa ttgagctgaa
     1261 tgattactgc tttacgagat aaaaaggctt ctgtcagtat actatcgatt tcttgcaatg
     1321 tcatttgttg tttaggaaga ggctgtttgc gtgcttcttc ttcatttttt ttgataccgt
     1381 tgactagttc ttccatcgca aaagcagtcc cccattttaa tccaaatggc cgatctcgat
     1441 actcgttata gtcaatgaat ttttttgctg tccgtctcat cttttccacc ttctaatcca
     1501 ctcataccac ctgcatgccc gccaacgaga gaactacgtg cgatggcgcg tcctccttgc
     1561 aagagactat gcgcatggat gattgattga aagccgtatt tttttctaat cgtatcgaca
     1621 acaaaatcaa ctttcgcttc atttatctgc ttttcaggat cttcaaaaag gttaagctgt
     1681 aaacctgtcg cgtatacgag gtcggaacag ttcacgccta cacgccgaat atcctggtag
     1741 tggtaatgct ggtggaaaat ggtcaataaa gcatctgcta gatctctgct agcatttgtc
     1801 gcttcgattt tcatttgctg gtgaaacccg gtcttgccat ttggatcaag atagcctaaa
     1861 gagtatccta cccataagct gactacttgt gtttttgctg cagatcgtct caagcgagtg
     1921 ccgacttggt cggccatttc tttgataacg atctcgattt cttctcgtct tgtataatct
     1981 ctatctagga tttgactatt tccgatactt tttgattttg gtgtatattc ttgtcccaaa
     2041 aaacttcgat cgatgcccca agcatgggca taaagctgag taccgatgac ccctagcttg
     2101 cttttcaagt gataataatc cgcgtgagct aaatcataaa cagaatgtat acccatttgt
     2161 ttcagacgtt ttgctgttcg tttgccgatt ccccaaaagt cagtgatatc tgagattttc
     2221 cataactttt ctggtacatc ttcataacgc cattcagcaa acatttgctt tccgtctttt
     2281 gagccattgt ctaaggctag tttagcaagt agcgggttat cgccaattcc tatagtcgtg
     2341 tagattccgg tctgctcaaa cacatcttta tggatcagtt tagccatgtc gtacgcattt
     2401 gttttaccaa agagtgccat gctatttgtt acatcaagga agctttcatc gacggaatag
     2461 acatgatggt tcgcttcgtc tgcatatttt ttatagatag aattgatttc tctgttcttt
     2521 tccatataat aggccatcct tggggcaaca atatataaat cacttggata ggggaaggga
     2581 agatctctgg cacggctgat attggtgatg ccgtaagctt tttttgctgc aggactggaa
     2641 gccaagatta atccgctacc ccgtgtttct gtcgaatcgg acggatagga catgactact
     2701 aatttagttg taaggggatc aagatccttt tcaacgcact ctacggaggc gtaaaaagat
     2761 ttgcagtcaa tacataaaat atctcttgaa ggttcttttt cataaacaaa taccggattt
     2821 tgattcgccc acataacaat cacctcttca acacaattag aacacacgtt ctctttccgt
     2881 gtcaataaaa aacagcaatt ttcaaatacg agcaaaaggc tcacatttgg aaattgctga
     2941 gaggttattt ttatctattt gtttttagct gagatcgttg atttttttat caaacatgaa
     3001 cagcttctgg atcgatcttt gttgtcccat ttccaatgtg tgcaacataa aattccggag
     3061 catagccgac aactttcatg tattcatcat agacattgtt tttgaaggca ggtatatttt
     3121 cttctttgac aagagcaatc gcacagccac caaatccagc tcctgtcatt cgtgcaccta
     3181 agacaccttc ttgtttttga gcagcatcta ctagtgtatc taattcgatc ccagttactt
     3241 cataatcata gcgaagagag tggtgagaat catttaacag ttgaccaaat gcttctaagt
     3301 tcccagcttc taattcagct tttgccttca aagtccgctg attttcagaa acggcatggc
     3361 gagctctgcg tatcaatgta tcatcgccga tttggtcaat agttgattca aattcttctt
     3421 catctaagtc tcctaaagaa gagataggca aagttttttg caaacgtgcc aaggcttctt
     3481 cgcattcgct acggcgttca ttgtattttg aatctgccaa ttcccgccgc ttattcgtat
     3541 tcatgatgac gattgcataa ccatctagtt tcactggaac catttcgtat ttcagcgtat
     3601 ttgtatctaa taagattgca tgatctagct caccgaagcc gatggcaaac tgatccatga
     3661 tacctgaatt gacaccgatg aattcatttt ctactttttt acctgtttgg actagttcca
     3721 agcgatctgt ttttaaatca aacagatctt ctaatacaac acctactaat aattctaatg
     3781 aagcgctact ggaaagtcct gcaccattag ggattgttcc ttccactaac aactcaaacc
     3841 catgatcgat tgagtgtcca gcttctttta atttaaggac cattcctttt acgtaatttg
     3901 cccagtttgc ttttttatca tattctagct catctaaagt aaaactaatg acaccgatat
     3961 cgtcaaagtt tgtggaataa actaatattt tattatcgtc tcgaggtgct gctacacctt
     4021 gcgttccata agtgatggaa gcggggaaga catggcctcc attgtaatct gtgtgttcac
     4081 caatcaaatt gatgcgtcct ggtgagaagt attgtgtagt ttttttaggt ccgaatgttt
     4141 cggcaaattt tgtgtttaag cttcttttga tttccatgat gagttattcc tttcttgttt
     4201 tgattttgaa ttctgtcact gtttcaaata ctgatccagc ttttaaatga atcgaaccaa
     4261 atgctgagaa gcgctctgca cctggagctg tttgagtctc aaatgtgatg ccaccgtgat
     4321 gtgccagttt tcggttgcgc atttctggtg tttctgtgcc gaaatttgca gtgaaaataa
     4381 ccacactaga agcatctgta gcaataccta cttggattct tttatcgggg ctagtcaagc
     4441 gagcggcttc tttacccaag ccggtttctt ttaagaaaaa tggatgatcg attccgtcaa
     4501 caagattttt ttgatccaaa tcactagcaa aaacggacgc taatttttta ggtatttgaa
     4561 aatcgaaagc acttcctgca gcattttctt ttacccctat aggaatacta tctgttcgca
     4621 aaggagcata agcttcgcta ttgagccaaa gctcatgttg atcgattgac tgagaagcat
     4681 ctcccgtcaa gttgaagtaa acatgatttg tcggattgaa taaagtatct tggtcactgg
     4741 tgccgcgaga ggtcactcgc cagatattat cttttgtcaa agtatagcgg atttccacat
     4801 ccattgtacc tggaaaacca tgttctccgt cgggacttgt agtagtaaaa atgacagaag
     4861 cttcattttc gccattcaaa atgacataag accatttttt taattcaaat ccaggagcac
     4921 caccatgcag attatgtcca gtttttggat ctgtggctaa ttggtaagtt tttccattga
     4981 gagaaaagcg gccattttcg attctccctg cggtgcgacc gatagaagca ccgatatacg
     5041 gatctttttc gatatattct tcagcggtgt caaatcccaa aactaattct cgatcatttg
     5101 attttaaact gacgattcta gcacctaaat cagagataga aagtgctagc ttgtttttgt
     5161 tctccaaggt tatcaagtga taaccttggc caaaatcttt ttctgtaatc ttcattttca
     5221 agatcctttc ttaatgggaa ggaactactg catcgtgaag ttcatcttcc attgtatcag
     5281 ctggagccaa tttgttttcc aattcctcaa cgatttgtgc atgttttttc tctgtcaatg
     5341 taatcttcca agcaaaaata atagcggaga aaatcatcaa agcagcagga gcatagaatg
     5401 cataagcttt gaaagtcgtt acgccatgag cagtgatatc agcagcagta gcattaccag
     5461 tcatcccaga agcgatagcg acaaacccta cgatcccgtt agacaaagca ccagcgattt
     5521 tatctagtaa agggcgaatt gatagagtaa cagcttcgtt acggatacca tttttccatt
     5581 gaccatattc aacagagtct gtgatcgtca tcaaagcgga taagaaaatc aattgttgag
     5641 ggaagtagaa caatactagc ccgatgataa cgattggcaa acttgaacct gcgatcgtaa
     5701 agaaaaagta agaagaaatc atcattgcga tccctccaag gtaaacgtag cgacgagaga
     5761 tgacgcgtgt aagcattggg aatagtggaa cagctactaa accaagtaag gcagcaacta
     5821 gaccaacgat ccaaaattca gttggttggc caagtacata tttgaagaag tagaataata
     5881 cggcagttgt tgctacattg gcaatcgcat aagcgatata agcaagggca agccacatca
     5941 actgatcatt ttgagcaact gctttgaaca catctttgat cccagctttt tcattttgct
     6001 gacggatcgc agtatcgact tcttttgtgc ccaaggcagt gataatcgca gtaccgcctg
     6061 aaataacagc agcaatcaca ccgaaccaga accagcctga agcgccttca atatgtttat
     6121 gagtaacaag ataggtaaag aaagtaacga tagggacaac gactagagta gtaccatttg
     6181 cacctagaga agagccgaaa cgagcaaacg tagcaaattt ttcacgttcc tttgtttccg
     6241 tactcaaagc tggaatcatt gaccaaaaag cgatatcctt aaatgaataa aaactatcca
     6301 ataagatgaa aacgattgca aataatatga taaataaagt cgtattatga gtagctaagc
     6361 caaagaaatt ggtaaagatt gctaccaaga gtacggaact aacaacgccg ccgataacta
     6421 accatggttt gaatttaccg tattttgtac gagtattatc gatgatgcca ccaatcaaag
     6481 gatcaaaaat aatttcaact aagcggatac ctacaactaa gcttgtaact gtagcaatca
     6541 tttttgcatt cgttgcctta tctgttcctg cgaacatctg gcttgtcacg aataccataa
     6601 aataagtgct taatgtaaca tagaatgtgt cgtgtccaaa agcgcctaaa gcgtaggaca
     6661 cacgttggat cattttgctt ttcataatct gtattcctct cctgttcaat atataatact
     6721 aagtaaaccg gttacaacga ataacaaaga tcttttaatg acttaaactt aacaagaaat
     6781 cgcttccttt ttaatatatt tctgtgactt ttatttaact ttttgttgca ttatactaaa
     6841 ggagtaagga gggatagacg tggagcaatc gattttttta aaaaaggaac atcaacaagt
     6901 agaaaaagaa ttgtattttg atttttgtgg tttctctaaa acgatgccgt ttcattcttt
     6961 tggtcctgct attcgcaatg attacatcct gcatgtcgtg atggaaggca aaggatctta
     7021 tcatgtgaaa aatcaacaat tccaattgaa aaaaggtgat ctgttcttga ttcgtccagg
     7081 agactcgacg ttttacttat cagatggaga agatccgtgg atgtattgct ggctttcatt
     7141 tggtgggagt gctgcagaag aaatcattcg gcagtcgctt ttcagagaag accaatacac
     7201 gatggtctct tctgacatac aaaaatatat cgacattatt ttagaatgca tgaaagatac
     7261 ggaagaaacc ttggaaaatg aattacagtt aacagcatta acctaccgtt ttttatcagt
     7321 ccttttaaaa gatggcggaa aggtcagcgg tggtgtacaa aaggttcatt cctctttagc
     7381 tgttgaaaca gtcaaataca tcgaggcaca ttatgctgaa aaattgtctg ttgaggaaat
     7441 cgcccagcat ttagcggtca atcggagcca tttgtcgcga gtattcaaaa atcatatggg
     7501 taccagtatc aaggaatatt tgatcggggt acggatcaat cgaggggcgt ttcttctttc
     7561 tctgacggat gaatccgtgg aaagtattgc ctatcaagta gggttcaata gtctagtcgt
     7621 atttagccga atgttcaaaa agagtaccgg agaaacggct acgaactatc gtaaacgtat
     7681 gaaaaacgaa aattttaaga atattgcttt ggataccttg aaaaaacagt tggaaaaaca
     7741 aaccgcagtt tcttggacaa cctgattagt ctggttaaaa tgacaagcat cggcaaaaga
     7801 aacttgtggc ggttatgcta cactgtaaag cgaagactaa aaaggaggaa aagggatgcg
     7861 tacacttatc atcattagcc atccaacgat cgaagaatct ttaaatcagc aatttttcaa
     7921 agaagcttct gctggtttac cagctacttg gcatcatttg gaatcaagtt atccagacgg
     7981 aaaaatagat atagagactg aactacagct cttggcccag catgatcgga tcattttcca
     8041 gtttccgttt tactggtata gttctcctgc tcatttaaaa atttggcaag ataatgtctt
     8101 ggaacgagca gcacatgtat taaaagggaa agaactagga atcgtattga caacaggtgt
     8161 ggcggaaaaa gaataccaag ctggaggaaa agaagaatat acgatttccg aatttttgag
     8221 gccgtatcag cgaatcgcaa ataaattcca tatgacctat cttccgccat tcgttttagc
     8281 acaatttatg tatttatccc aggaaaaacg ttgggagaag ctaattgctt atcagcagta
     8341 tctctcattg gaaggcaaac catcgctgac tcagcggatt gactggttta ttcaaagagt
     8401 acaggaaaat cagaaaatgc aggaagaaga atctgaaaaa caagcatata tcgtagaagc
     8461 actgactgag gcaaaagagc agatcgaaga cttgaccttt acactgcaag aaatgaaagg
     8521 aacttcatta tgacaaatga ttggctgatc caacaattag atgaattaag cgaaaaatca
     8581 gaatatcaag accgtgcgct cttatatgaa ataaagaaac tgatggctga gatagacgaa
     8641 cgaacaatgc agctgcaagg agaaatcgac ggacaatctt ggaatcatct caactggtga
     8701 aattttctta taaataacaa agataaaaat gtgcacagcg acaattattt gctacaataa
     8761 gaatatgaag cgtttgataa cgctattata cataaaaatt ggtggtgaaa aaaatgtatg
     8821 atgaaaatga gtggggagaa aatgttcgga tgacccgaag aagacagcaa aaacaggcag
     8881 ctcgtaagcg gatgatcatg attggggcag cagttgccgt gtttttgatc atagcaggag
     8941 gagttgcggg tctatatttt catggaaaaa attcgtcaga agcgaaaagt acgaccataa
     9001 cggaaacaac gacaaagaaa acagagacga aacaaacaag tgagtcatca actaaaacaa
     9061 aagagacaca aacttctgaa gaaaaaacag caaatggttt gccagagcaa cttcaagcaa
     9121 tcgtaaatga ttattcgaaa aagatcgaag aaaagacgcc tgcattgatt gaagaatacc
     9181 agacagaaat tcagggaaat caagagggaa tagctggttt atcagcagtt gctaaccaaa
     9241 aagcacgtga gcttcaagca atctccgacg aagggatcag aaaattacgt gcagcgtatc
     9301 aggcagcaga aaataaagac ggtgtagact tagatacttt gatcaatcaa ttatcggcta
     9361 attatacgaa tcatgtcgct aagatttccg atatttattt gcagactagc gcctctcttc
     9421 aagcagagtc aacaagcaca caagacacaa catcttctga ttctgagaca gcagagtcgg
     9481 tagaagcttc tgaaatgcaa gctcgaacaa cacaagacac aacagattcc tctgaaacta
     9541 ataccaacag cgcgacttca caggctgcga caaccgtagt tttggaagga gaaggtccaa
     9601 atcaaatagc cgaacgaact ggcgtaccag tagaaacgat cttgtcgctg aatgggatga
     9661 gcatggagaa ttatttcctg actcccggtg agacgctaaa attgaattga taaaagcaat
     9721 aaaaaagaac gaagcttcca ctaaggtgaa gcttcgttct ttttttattt tccggttctt
     9781 ttttcgcgta caaagtaatg gatcgtcatg taaagcaaga aaacgagtcc aataatcaat
     9841 gagattctcg tttcaggatt gataaacatg aaaatcaata cgatagctaa aaaagcaagt
     9901 gtcacataat tcgtataagg agatagtggc attttaaaag gatgatcatg cattttatct
     9961 gcgtgcttct ttttgaagcg aatctcgctg atcaaaatca ctacccaagg aaccatacca
    10021 ggcaaaatac ttgagctgta tacgaaaacg aaaatatttt ctgactggtt gatgaacatt
    10081 ggtagaagat aatttaagat cagaccaatt aagattccac ctgagattgc tacgacagga
    10141 taaaaaggcg caccgtgacg agatagtttc agaaattttt tagacatctt tccatttatg
    10201 gcaagtgtat agaccatacg acttgcgctg aaaatccctg aattgcaacc tgataatgcc
    10261 gctgtaatca cgacaaaatt aatcagtcct gcagcaaatg taatgccgac tttagaaaaa
    10321 gtttgtacaa aaggtgaacc aatctcactc aattgattcc acggatagat actcacaatg
    10381 ataaaaatag caccaatata aaaaatcaag attcgaccga tagtggactg gacagcttca
    10441 atgatcgtct ttttaggatt ctctgcttca ccagcagtca ttccgatcag ttcgattcct
    10501 tgataggaag caacaacgat cgataatgca aagaaaaatc ctttgacgcc tccagtgaag
    10561 aatccaccat ttttccaaag atttgaaatc cctactgcct gaccatgatt accaaatcca
    10621 aataagatta ccccaagacc tgcaatgatc atcaaaatga tcgttatgac tttgatgatc
    10681 gaaaaccaaa attctaattc gccaaacgcc ttgacagaaa taagatttgc cagcataaga
    10741 aacaagacga caatgattcc gggaataatt gccggcaaat caggccacca ataattcata
    10801 taagatccta cagcaatcac ttcacttatt cctacgacaa tatattgaaa aatattactc
    10861 caagcggtca agtagccagc cacaggatgg atgtactcgc tcgcgtaatt tgcaaaagaa
    10921 ccagtcgaag gatcaacgta aagcatttcg cctaaagcac gcataatcaa atacaaaaag
    10981 aatcctgcaa tcgcataggc tcccatcact gagggccctg tccaacgtat ggttgaggag
    11041 gcacccataa acagaccgac accgatcgtt ccacctagtg cgatcatttg gatatgacgg
    11101 gcactaagac ttcgagttaa cttttcttct tccatacaaa aactcctttc gttgttgagt
    11161 caacaagaaa aaataatcaa acgtttcaac acatgttttt cattattagg ttagccttat
    11221 tgaactcttg atgtgattct acagaaaatt ttcataaaat gcgaggaaaa ttcgaaaaaa
    11281 actttttcat cattctactt gaaaatgctt tccattttag ttaaactata tttaaataaa
    11341 aagttaggta aacctaaaaa ggagtgtaag atttggaacg tacggccatt gtaattcaag
    11401 acctttcagc cgcttatcaa gggaaaactg ttttgaaaac tatcaacttg acgataaatc
    11461 cacaaaaaat taccggtatt atcggaccaa acggtgcagg aaaatcaaca tttatgaaat
    11521 ctttgctaga gttgatacca gctactactg gtaaagtcac cttttctgga cagccggtaa
    11581 actcagtccg taaaaaaata gcctacgtgg aacagcgaag tgaattggat ctttcctttc
    11641 caattgatgt attaggcgtt gtacttttag gaacatatcc atctttacga attggacaaa
    11701 gacctgggaa aaaagaaaaa gaacgtgcaa gacaagcttt gaaaaaagta gggatggaag
    11761 aatatgccaa aagacagatc agcgaactat cgggtggaca gcttcagaga gtttttattg
    11821 caagagctct agcccaagga gcagaatgga tctttttaga tgaaccattc gtagggattg
    11881 atgcgttaag tgaacgaaag atctttgaca tcttgcagga attgaagaat tcaggaaaaa
    11941 cgattttgat cgtccatcat gatcttcata aagtagaaga atatttcgat gaggttattc
    12001 ttttaaataa acagctgatc gcttccggtc cagtacaaga agtctttaca tcgaagaacc
    12061 ttcaattggc ctatggtgag atcatcagac atttagtaaa aggaggagag aaaaaatgat
    12121 ccaatccttt attgatggat taatgaatta tcaattcctg caatacgctt tggtcacttc
    12181 catgcttgta ggacttgctt ctggagtcat cggttctttt attattttac gagggatgtc
    12241 tttgatggga gacgctatct ctcacgcagt tcttccaggt gtagccattt cttatatgtt
    12301 tggttttagt tacatctttg gagccactgc ttttgggatg ctaacagcag cagtgattgg
    12361 tttcgtgact cagcggagcc gattgaaaaa tgatacagcg attggaattg tctttagctc
    12421 cttttttgca ttagggatta tcttgatttc atatgcccag agtgcgacag atttgtatca
    12481 tattttattt gggaatgtct tagctgttag ggaaagcgat ttgttgctga cagcactcgt
    12541 tagtggaatc gtcttgatat ttgtcttttt cttttataaa gaattgaaga tcacatcatt
    12601 cgatccgaca atggcaaaag cttatgggct gaacacatcg ctgatccatt atcttttgat
    12661 gttctttttg acattagtgg ctgtagtcag tttacagaca gtaggaacaa tcttggtgat
    12721 tgccatgttg atcacaccag ccgccacggc ttacttgcta acgaaccatt tactgaaaat
    12781 gatcattaca gctgcaggaa tcggtatgct aagtgcagtt gtcggtgtgt ttttcagtta
    12841 tagttacaat tggccatcag gagctacgat cgtgttagca tgtaccgcat tttttatcct
    12901 tgctttttta ttttctccaa caaaaggaat tttatttcag aaaggaagca gatagatgaa
    12961 aacaaaaaaa tcactctttt taatcttggc agttagtttc ttggtattag ccggttgcgg
    13021 aaagcaggct tctgaccaag cggatgaagg aagtaaagaa aagttgtcag tcgtggctac
    13081 caattcgatc ttggcggaca tggcaaaaga ggtcggaaca gatcaaatag atatccacag
    13141 tatcgtacca gtcggaacag atccgcatga atatgaagta ctaccagaag acatcaaaaa
    13201 ggcaagcgat gcagatgtta ttttatacaa cggtttgaat cttgaaacag gtaacagctg
    13261 gttcgataac ttgatggaaa cggctaaaaa agaagaagga aaagattatt ttgcagttag
    13321 caaaaatgta gaacctcttt atttaactag cggtgaagaa catacaaaag cagatcccca
    13381 tgcttggcta gacctatcta acggaataaa atatgtggag gaaatcgcac gtatattctc
    13441 tgaaaaagat gcagaaaatg cgacactcta taaaaaaaat gcagaagcat atgtggaaaa
    13501 actaaaagaa ttagatacac aagcgaagga aagttttgct tctatcgaag agaacaaaaa
    13561 attattagta acaagtgaag gggcattcaa gtatttttca cgagcatatg atttgccagc
    13621 agcttatata tgggagatca atacagaaag tcaaggtacg cctgatcaaa tgaaagcgat
    13681 cattgatcag ataagagcaa aagaagtacc ggttttattc gtggaaacca gtgtagactc
    13741 aagaagcatg gaacgggtag caaaagaaac gggtttgaaa atctatgata aactgttcac
    13801 tgattccata gcaaaagaag gagaacaagg agattcttac tatcagatga tgaaatggaa
    13861 tattgaaacg atccatgaag gtttgtcaca aacaaaagaa agctgaaaag tgcaaggtct
    13921 agcttgagga gcagaccttg cattttttat aagagatatg aaaaaaaatt atgctgtttt
    13981 tcgtcgtaca gatacctttt ttgaagaaat tccgttatag ttagagtaag gaaggaaggt
    14041 gtggctatga attttacacg taaagataaa tatacgatta aagaagaagt ctttcgattg
    14101 atcagccagc aggaaaatac cgacaaggat caagaccttc aatttcttga agcagaatta
    14161 gctaaattat tcatagcaaa tcaaaatgag acaacagaac cagatgaaga gctgtacata
    14221 ggcaaatggt tgtttgatga atttcagcct ttagatgaaa ataccggtat cggttatttt
    14281 gtgttccttt ctccagtcgg cttcacacct ggcgtattag ttaaacaaaa cgacgaaata
    14341 accgtcaaag catggcctgc attattttca gtcatcaaag cagaaggagt aatcaaaaag
    14401 atccagtcgg cctattataa aagtcaattt attgccaaca gcaaattaat gaatatgttt
    14461 tataatcgtc tagtagaaat tgctaggcct gggaaaatcc cgcaaacatt cacgtacaca
    14521 cagcaaaaaa atctctatga agaagtattg tattcccgcg atctgtctga gttttcgcca
    14581 tgggaagagg taaggctcca tgaactgtta agtacaaaac cagtttcatt gaattttttt
    14641 aatgcattag ataacaaaag aaaattaatt tcaacaaatt attgggaagt atttgctgca
    14701 agaaaaaaat ggatggaaga aaagaagatt gccgatcagc agatccagtt tgtccagctg
    14761 aaaaaagaaa aactatatta tgggttatac agccgttaac agcaagcgag gaagaccatg
    14821 acattaaaag aagaaatcat acaagaaagc aaacgattag gaattgacaa aatcgggttc
    14881 accacggcgg aaccttttga ttcattaaaa gaatcgttgg aagaacaaaa agcagctggc
    14941 catacttctg gctttgaaca tccaaatatc gatgagcgtc tttatccaga aatgacattt
    15001 gatcagccga aatcgatcat ttccattgca cttgcgtatc ctactaggat ccatgaagaa
    15061 gtgcctaggg atgaaaaacg cgggcagttt gctcgtgctt cttgggggat cgattaccat
    15121 gatatactca gagaccgttt gaaccgattg atcgaattca tccaaacaag agcagaaaaa
    15181 tggcaaaaag aagaggaatg gcgtctagct ccgcaagtag atactggaga actgatcgat
    15241 gtcgctgtag cacagcgagc tggtttagga tttattggac gaaacggatt gttgatcact
    15301 gaagagttcg gttcatttgt ctatcttggt gagatcgtta ctaatattgt atttgagcct
    15361 gataaaccag gagaatttgg ttgcggtgac tgtatgcgct gcgtgactgc ttgtccgaca
    15421 aaagcattgc ttggtgacgg aagaatgaat gcccagcgat gtttgtctta tcaaactcag
    15481 acaaaaggga tgatgccgga agaatatcgt aaaaaaatgc gaaatgtcat ttatggatgt
    15541 gatatctgcc agttagtctg cccttacaat caaggaaaag attttcattt tcatgaagag
    15601 atggaaccaa aagtagacga agtatatcca aaattaaagc caatgctttc gatgtctaat
    15661 aaagacttca aacagcaatt tgggcattta gctggttcat ggcgtgggaa gaaacctttg
    15721 cagagaaacg cattgattgc tttagctaat ctaggtgatc gaacggcatt gcctgagatt
    15781 tcagaatgtt taacagatgt ccgccctgtc atcagaggaa cagcagcttg ggctattgga
    15841 cgtctaggaa caaaagaacc ggaaaaatgg cttgatgtat tagatgaaat attagctaaa
    15901 gaaacagaag aagtcgttat aaacgaattg gaacatgccc agaaattatt gcggagaaag
    15961 ttaaaataaa aaaatagtta acattattca aagtttttgt ccagtaattg ttgtaacaat
    16021 tactggtttt tttgtatttt ttacattttt ttaagagaat ttgataaaaa tcaaaaattt
    16081 ttttgctttt ttttatggat tattgcagtg aatgttacag aatctgcgtt tttcattcaa
    16141 aaataaaaaa acatagaaaa acgattactt tgcttataca acaaggcttt tatggttttg
    16201 tatcaaaaaa atatttgtt
//
LOCUS       DARBEC010000063        16212 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.065, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000063 DARBEC010000000
VERSION     DARBEC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16212)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16212)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16212
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.065"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..469
                     /gene="scm"
                     /locus_tag="SCL80_001955"
     CDS             <1..469
                     /gene="scm"
                     /locus_tag="SCL80_001955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEG1107810.1"
                     /translation="TSASSDSTDTSTTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTS
                     DSTDTSASSDSTDTSSTTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNF
                     NLTNKTSNDNGNKSKRALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(624..1610)
                     /locus_tag="SCL80_001956"
     CDS             complement(624..1610)
                     /locus_tag="SCL80_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEG1107811.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(1633..2199)
                     /locus_tag="SCL80_001957"
     CDS             complement(1633..2199)
                     /locus_tag="SCL80_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEG1107812.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(2213..2629)
                     /locus_tag="SCL80_001958"
     CDS             complement(2213..2629)
                     /locus_tag="SCL80_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEG1107813.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(3086..5020)
                     /locus_tag="SCL80_001959"
     CDS             complement(3086..5020)
                     /locus_tag="SCL80_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1107814.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGNSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            5301..6257
                     /locus_tag="SCL80_001960"
     CDS             5301..6257
                     /locus_tag="SCL80_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEG1107815.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            6347..7450
                     /locus_tag="SCL80_001961"
     CDS             6347..7450
                     /locus_tag="SCL80_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1107816.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            7463..8416
                     /gene="menA"
                     /locus_tag="SCL80_001962"
     CDS             7463..8416
                     /gene="menA"
                     /locus_tag="SCL80_001962"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEG1107817.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFDLVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(9230..10576)
                     /gene="gor"
                     /locus_tag="SCL80_001963"
     CDS             complement(9230..10576)
                     /gene="gor"
                     /locus_tag="SCL80_001963"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEG1107818.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(10710..11963)
                     /locus_tag="SCL80_001964"
     CDS             complement(10710..11963)
                     /locus_tag="SCL80_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1107819.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(11969..12892)
                     /locus_tag="SCL80_001965"
     CDS             complement(11969..12892)
                     /locus_tag="SCL80_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEG1107820.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(13113..15605)
                     /locus_tag="SCL80_001966"
     CDS             complement(13113..15605)
                     /locus_tag="SCL80_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1107821.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(15612..16079)
                     /locus_tag="SCL80_001967"
     CDS             complement(15612..16079)
                     /locus_tag="SCL80_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEG1107822.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
BASE COUNT         4763 a         3390 c         2885 g         5174 t
ORIGIN      
        1 cacgtcagct tcaagcgaca gtacggatac atctaccaca acaagtgata gcacggacac
       61 gtcagcttca agtgacagta cagatactac ctctaccaca agtgatagct cagattcatc
      121 taccactaca agtgatagca cggacacgtc agcttcaagt gacagtacag atacatcctc
      181 taccacaagt gatagctcag attcatcaac ttcaagcgat agtacagaca cgtcgtctac
      241 cacaagcgat acacgtcatg caagtgtctc cttctcaaaa acaagttcgg ataacaggac
      301 gaactttaac ttaactaata aaacaagcaa tgacaatgga aacaaatcaa aaagagcact
      361 tcctaaaact ggaagtcaat cgaataattg gataacactc gcaggcgtta tattacttgt
      421 aatcggttta agaatcactt ttatgagtta taagaaatcg aaaaggtaaa aaaatattca
      481 agataaaaaa gagcaacgtc gctaacggac gttgctcttt atttttgctt tttatcataa
      541 atacaagaac tacaattcaa tttgtcaaaa aacacgtaga tttccttctg agaaatttat
      601 ttacgtatcg aacctttccc tattcagtct ttgcgatcca aaatcagttg ggtaagggaa
      661 aagagattct cttttgttcg aagagaggtg gcaggtaatt tctttatttc cttcaaagcc
      721 ttttctgtgt aagaggccgc taacttccta gcttcttcca cgcctcctgt atgttcaacg
      781 attttgcgta cggcgtcgac atcttcttct gtcattgccg ctttcttctc taaataaggt
      841 agtagctttg gtttgttttt ttgcaaagag tagatcaacg gtaaagaata gaccccttga
      901 cgcatgtctt ctagtacagg tttgccgatt tcctggctct cttttgtata atctaaaata
      961 tcatcgataa tctgaaaggc catcccgata gaaagtccga tatctcctgc tttttttgcg
     1021 aagcgctcgc ttgttccact ttcaaaagcg ccaacagaac aacttaattg gaacagttca
     1081 gctgtcttac ctgaaatatt ttccaaatat tcttttacag aaagttcata attatagcga
     1141 tcatccatct gccctaattc gccattcaat actttttcca tactgcgaga attcaattgg
     1201 agacttttca aggaagtgga gtaatcagac aatagtttaa aacagcagac aaataaatag
     1261 tctcctgcat agacagaaac gacattacca aactgactac ggatattagg ctgaccacgt
     1321 cgcagatccg cttcatcaac gatatcatca tggatcagtg tggccgtatg gagcatttcg
     1381 atagaagctg ccaaagcgat ggctttgtct cgatcttgct ctggtccaaa ttgtgagaac
     1441 aatagctgat aggcaggacg caacagcttt ccgccggcat ggatcgtttc tttgatggct
     1501 tgttcgacag gtttgttttt caggcggaca gtttcttcca tccgttttag tgtttctgtc
     1561 aattctttac ttaaagcagg atagttttcc cacattggat gtagcttcat aaaaaaactc
     1621 cttgtgtgac gattattttt tggtaaacat tgggctccat ttttgtttgg ttccctgctc
     1681 ataatctttt tggaatcgtt gaagtgtctg cacatgtcgt aaaagataat tcgctgcgat
     1741 cccgattgca atccctgata aaatccctaa aactgaaagg actggcaagt acaacatgac
     1801 tgtccatgat tgtgcaatga aagaagccat caaaagctgt ccgacattat gcatgaaacc
     1861 gccggccgca ctaattccga tgatactgac acgttttggt cctaattgct tgaccaataa
     1921 cattccaaaa taactaagca aagcaccact catactataa aggaatgtgg aaatcgttcc
     1981 gcctagcaaa gtagtcagga atagtcgtag ccaaagtagt aagaagctgt cttgtttttt
     2041 catggtaaaa atggcaacga tcgtgattag attggctaag cctagttttg caccaggcgc
     2101 aaaagaaaaa ggataaggga tcatattttc caataaacca atgatcactc cttgtgcaac
     2161 aagtagcgaa atataaatca ttttttgtaa tttactcatg gtgtgtgaag gtctaataaa
     2221 tcagactgtc ttcgtcacct ccatctgttg aacggacttc gatcactaat ttatgtggca
     2281 aacagacgat ggtttcgcca tttttcgttg cccagcctcg acgtacgcag atctggtcat
     2341 cgcaatctgc ttcttttatc cgtactcgat cgccatctat ctcgatcaag ttataatctc
     2401 cgtgttcatc tttgtaagta tatgtgttgc tctcttctcc agccatcaaa gggaatgttt
     2461 tgatttctgt gccatccaca cgcagtaccg cttctttttc tgctgttgcg gtgttttgct
     2521 gaagaccaaa aatcatgact ggtaaaaaag aaagaacagt caaaagcaaa ataataaaaa
     2581 catcccaagg tttgagcaga cttttttcag caaattcttt aaggctcatt tttgttctcc
     2641 tttttccaga ttaactcacc caattttagc ataaaaacaa gaggttgtga cacctgtgtc
     2701 ttgctttgag taataagaac gaagaataga aaacagcttt ccatgttttc taattctttg
     2761 ggcttattac cgagaagcag caggtggaac accgtttata acaagaggtt gtgacagaag
     2821 tgggcaactt caagaaataa gaaggaattt acgaaaatta cttttcaagt ttttgtgaaa
     2881 tttcggctta tttttcgaag aagctacttc tggaacaccg tttataacaa gaggttgtaa
     2941 cagaggttgt gacacctgtg tcttgctcat ggaggcttat ctcgggcatc ttattccttt
     3001 aattttacat aaaaaggagg ctgtgacggt ttgtctcagc ccctttctat tgaaatcttg
     3061 cattcaaata acgttcttta tctatttatt tcttgccgta ctgggaactt ggtctaccgt
     3121 accaccaatt acctagtgta cgttgtagcc atggaattac ccagcgatcc aacccaatcg
     3181 cacgtccgga accgttcatc aatgcgaaag caacaaaaat gaaccaaatg tttacccagt
     3241 aaaacatgcc tgataagcag aacgtgatca ccaatacaat cgttgtcgca ctgcatagcc
     3301 atgtgaataa tccagcaatc aatgctaatg ccaaggcaat ttcaaagaat accatgaatt
     3361 tttggaagaa cattgctaca tcattgtttg gcatgatcac ttgcatgatc tttgtgaacc
     3421 agtctggtga cttctcaatc acagccattg gttcttcacc gtatgcataa ctcaaaccaa
     3481 aatgtgcagc ttgagtcgct gcttctccgg cacttgcccc tgctccgctg gcagctgtgg
     3541 ttgcatcacc agcagcagaa gcaccactgg caacatctgt tacattgtat tcccatgtcc
     3601 aagggaacac actgtttcca gtgaaccatg atccttcacc aaaccaagta cttggtttga
     3661 acacttcacc attcccaatg atttttttgg aagcttcaac gaaccaaact aaaccataga
     3721 aaatacgaag tggcacactc cataagacat tgccgtaacg agaagaatgt cctcgagtca
     3781 cgttgcgatc atctttgata tggaaaaact catgcattaa atattggaac atataataac
     3841 cagaacgaat atcaaagaag tatttcaaat tcacgatatg tttcattacg atagctaaaa
     3901 atccgcttaa atggatctta tcaaataggt tagctacccc ccatttggca ccaatagaaa
     3961 ccataaatcc ttgatagttt cctttaaatt tatgcttttc tccgcctttg atcgaagcca
     4021 cgatattcgc tgcggctgtg tgtcctgttt gttcagcagc ttgaacgatc tgcggtgtcg
     4081 gtgttcctgg gaattcttca taaaagacta aatcaccaat gatatagatg tttttatctt
     4141 cgtagccttt tgcctgcata tattcattgg cgatcaaccg ctgtccgcgt gctgattcta
     4201 atccaaagtc agcagcgtca gaagttcctt tgacaccagc tgtccaaatc agtgtgtgag
     4261 tcggaatgtt gcgtccgtct ttcaatttga tatgatcagc agccacttct acgattggtg
     4321 cattcaataa taattccacg ttgtgcttac gcaaatagcg ttcagctttt gcagcatcat
     4381 ttcgatccaa catattcaag atcgtcggca ttgcctctac gaccatcagt gtgatttcgt
     4441 ttggatctaa tttataatct ttggctaggc gatttttcca gtcgatcagt tccccaatca
     4501 tttcaatacc ggtaaatcca gaaccacaga caacgaaagt cagcatggct ttacgcagtt
     4561 catcatctgg ttccaatgca gcttttgcta ctgtcttctc aatatgttcc cgaatcctta
     4621 cagaatcttc aaatgaccaa agggtgaatc cgtgtgcttt gacaccaggt gtaccaaagt
     4681 cattcggctc tccgcccatt ccaaggatca gctgatcaaa aggataggaa cctaatttgg
     4741 tcttgacgat ttttcgttct ttatcgatac cgattacagt gtctgtgaca agttgtacat
     4801 tcttttgacg agcaaacaaa tgctgcagat cgtattggat tgcttctggt tctacacgtc
     4861 cgccagctac ttcatgcaac tcggtcatca tagtatggta cgaatgacga tcgatcagag
     4921 taattgctac atcttctttt ttcagtttct ttgccaagta ttttgtagct gaaacaccgg
     4981 catatcctgc tcctacaacg acaatatttt gctttgccat tgatcatccg ctccttatag
     5041 actatctata taatgaatat atgataattc ttttagtttg atacttatta cacaatctcc
     5101 atatattata tagatgttct gttcttttgt ctaactcctt ttggattttt ttcaaatttt
     5161 aaaataaagc gttttttcac gaaatttgct gaaagaaaac gtttgaaata ttacatgggt
     5221 tatttatcaa aaatagttga cttataggct aaaaagatta gaatgtataa tgaatcacaa
     5281 agaaaaattg tggactagaa atggaatggt ggggtattaa aatgaagaaa aaacgttttg
     5341 taactggatt tgccgtatta gcattttcag ctctagtgtt aggcgcttgc ggcgcagatg
     5401 acaacggaag ctcaaactct tcttctgaat catctacagc acaatcttcg actgcaaaaa
     5461 gcacaacaga atcatctgca aaagttgtag ctggcggaga tttgcaagac ggcacatata
     5521 aattagaaga aaaaaattat tcaaatggtt accgtgcagt ctttgaaatg gttgtaaaag
     5581 acggcaaaat cactgaatca aaatatgata acgtcaatga aaacggcgaa tcaaaaacaa
     5641 aagatgcaga atacaacaaa aacatggaag caaaatctgg cacaaaccca gaaaaataca
     5701 ttcctgagtt gaatgaccaa ttcttaaaag cacaaagcgc aagcggtgtg gaagtcgtaa
     5761 ctggagctac tcactcatct gaaagcttcc aaaactacgc acaacaattg atccaagctg
     5821 ctcaagctgg taacacagat acaatcgaaa tcgacaatgg tgctgatttg aaagacggca
     5881 cttacaagct tgaagaaaag aactactcta acggctaccg tgtgcaattc gaaatgacag
     5941 ttgctggcgg caaagtaact gaatcaaact ttgattatat cgacaaagac ggtaaatcaa
     6001 aacaagatga tacagaatat aatgaaaata tgaaagcaaa aagcggtaca gaaccaaaaa
     6061 cttacatccc tactctgaac gacgaattcg tcaaagcaat gggcgaagaa gatggctcac
     6121 ctgctaatgt agaagttgtt acaggtgcta cacacagctc acattcattc atcatgtacg
     6181 cacaacaatt agtgaatgca gctgaaaaag gcgacactca aacaatcgaa gtagataata
     6241 tcgtaacaga aaaataatca cataatcata ctaataaaag ttggagggtg ggacgatagc
     6301 cagattaggc agtcgtctca cctttcaaaa tgaagagagg aaaaagatgc ggaaaaaact
     6361 actattttcc ttaatcacac tgggcatctc ttttatggtg ctaactggct gcagcaactc
     6421 agcaaataaa acaaccactg aaagttcagc agctaccaag atattgaaag atccatattc
     6481 tgatgaacag ttcttgttag ggacttacgt tcgtatccgt gtgtatgatg aaggcaaaga
     6541 atcagcattg aaacctgcct tcgatcgagt aaaagaactt ggtgataaaa tcacgatcaa
     6601 tcaaaaaggt tctgagatcg atgaagtgaa tgagcaagct gggatcaaac cagtaaaagt
     6661 ttctgatgac gtctacacgt tagtcaagcg ggcctacgaa tatagccaag attctcaagg
     6721 cggattcgat atggcgattg gtgcaatcac acaattatgg cgaatcggtt ttgatgacgc
     6781 cagaaaacct tcccaagaag aaatcgatca ggcattgaaa ttagtggatt atcataagat
     6841 cgaactaaat gacaaagaaa aaactgttta tctaaaagaa aaaggaatga tcatcgattt
     6901 aggtgcaatc gccaaaggtt acattacgga cgaagtagtg aaagtcttga aaaaacaagg
     6961 agtgaccact gccatcgttg acttgggcgg taatgtctat gtcttaggcc acagcccaag
     7021 aggcgaaaac caagactgga cagtcggcat ccaagatccg aacatggctc gcggatctgt
     7081 tttaggttct attaaagaac ggaacaaaac attagttacc tctggaattt acgagcgtta
     7141 tttagaagta gatggcaaaa aataccatca tttgtttgat ccaaaaacgg gttatccgtt
     7201 cgacaatgat atcgcaagtg tcacgatcat aacagataaa tcaattgatg gtgacgggtt
     7261 gtctacagca gtcttttcaa tgggtgtgaa aaaaggactg gaatacgtcg aaagcgaatt
     7321 gaataacggg acggaagcga tcttcgtgac aaaagacgat aaagtatatg tgaccgatcc
     7381 aatcaaagac accttcaaat tatcggagga ctcccattac acgatgggtg accgttctga
     7441 actaaaataa ggagtacgaa ccatgacact aaaaatcttt ttagaagtag ttgaaatacg
     7501 aacaaaggtg gctagtgtat ttccttttgc ggtaggggta ttattctcta tcgctttttt
     7561 tcatgagata cattggctaa acactttttt attttttatt ggaatgctag tttttgattt
     7621 agcgactacc gctatcaata attacatgga ttatgaaaag gctcattctg aagtatataa
     7681 gtatgaagaa aatgtcattg gccgcagaaa tctatctcct gtattgattc gaaacatgat
     7741 tttagctatg attgcatttg tttttgttat cggcgttatt ctaacgatca atacgggttg
     7801 gcttttactt ttgatgggat ttatctgttg ttttattgga gtattctata cattcggacc
     7861 gattccttta tctcgaatgc ctttagggga aattttcagt ggggtgacta tgggccttgg
     7921 aatttttgcc atggtcgttt atgtcaatac attcggttca gatgtctatg atctgacagt
     7981 caacttttca tctggacaat ttgatttggt aggaaatctt tgggctatag cagcaatcgt
     8041 attagcttcc ttacccttgg tgtttacgat tgccaatatc atgttggcta ataatttgcg
     8101 ggatcttgac cgtgatattg aaaatcatcg ctatacacta gttttttata tcggacgtcc
     8161 tgcgggaaca gtcttattcc agctattgat gtatgcttgc tatttcgtga ttttaatggg
     8221 gttgttgagc ggtatctatg aatggccgat cttggtcacc ttcttgacct tgccaattgt
     8281 gaagaagcat ttggatcaat tcaagcaaac gttaccccag cctatcagtt tcagctattc
     8341 catcaaaaat atggtcttat tcaatgccag ttatgtattg gggctattac taacaatcat
     8401 tatgcaagcc atttaacagc atgaagaggt tgtgaaaaaa ctgccctgca tcaagtaata
     8461 agaacagcca aataaaaata gctccttata tttttcgttt ggttgggctt attaccgcag
     8521 atgcaagctt ttgaacaccg tttatcagga ggttgtgaaa aagtcgctta gtttcgagta
     8581 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaatttgg cttaataccg
     8641 agaaactggc ttttgaacac cgtttatcag aggttgtaaa atcagtgttt tgtcctgagt
     8701 attagagaaa caaatggaaa aatagttcct catattttgc cgttttttga gctaataccg
     8761 aaggacagct gattgaacac tatttgttcg gaaaaagtga ctaactatga cacaattttt
     8821 ttcacagtcc cttttttcac ttgatttgat tcgtaccagc gaacttgacg agaactttct
     8881 tggtttggta actatatggt attcttttaa catcctcgtt tgcttcttct aatttattta
     8941 tacaaagtca cactttttta aacatctgtt cttcgataca tttctatttc ttttaaacat
     9001 cgataccaat attttgaact taagagtgca taccaaatgg attcatttac tcttcgtcag
     9061 ttacgaagat aatcaaaatc tccaacttcc ctacttattt cgttttgaga attatcgaag
     9121 caatcttaca taaataacta aaaaacaaaa aaccagaaaa tcagtatact gattttctgg
     9181 ttttacatgg tttgttcaat aaaaagctgt tggaactgtt aatattaaat tatcgcatcg
     9241 ttacgaattc ttccgctcct gttggatgga tggctactgt attgtcaaag tcttctttag
     9301 ttgcacccat cttgatagcc acggcaaaac cttgcagcat ctcatcgaca ccaacaccaa
     9361 tagcatgtaa gccgacgatt ctttcttctt ttccgacaca gatcaatttc atttcgcatt
     9421 tttggcgata atcatttaat gcaaagtaca ttggggtaaa gcgtgaacga taaactttca
     9481 attcattttc gccatatttt tccaatgctt cttcttctgt caaaccaatt gttgcaattg
     9541 gtggatgcgt aaagacgact gtcggaacaa gtttatagtc aagataagaa tctttctttc
     9601 cattaaataa acgttctgat aaacgtctac cagctgcgat tgcaacagga gtcagatcta
     9661 atttaccgat cacatcacca acagcaaaaa tatgttcatc cgttgtattt tggtatttat
     9721 ctactttgat aaaccctttt ttgtccaatt ccacatttgt attttccaga cctaattttt
     9781 cggtattcgg tacacgtcca ccggcaaaaa gtacacaatc accagttaag gtttcaccat
     9841 tttcaaatag caccgtatat tcgtcattgt ttttagtaat ttctttagca atatgatgtg
     9901 cgtaagtttt tattccagct tcttggtaca tttggatcaa attgtccgaa agcattttat
     9961 caaacgttct aagtggtcgt tcttttcgat atgcccacat gacttcacta cctaaaccac
    10021 gaaatacacc tgaaagttca gccgcaatgt atccagcacc taaaacgatt gctctcttag
    10081 gtaactctgt taattcaaaa aatccatttg aatcaattgc gtattctcca ccaggaatgg
    10141 tcattttact tgcacggcct ccagtagcga tcaagatttt aggagcacga taggtttcac
    10201 cgtttacttc gatcgtttga ctatcgataa aagtagcgta accttttata gcctcgacat
    10261 gattgctatc taatcctctt tgataagcac cgtgtagaaa atcaatatat ttttctcgtc
    10321 tttctactag accggcgaaa tcaacatttt ttaaatctgc ttggataccg taacttgagg
    10381 catctcgttc aattgtttcc aaaatcgtac ttgcttgcca cataactttt ttaggtacac
    10441 aaccgacgtt gacacatgtt ccgcccaatt cattcgcttc aatcagcaaa acttttgcgc
    10501 catgcatccc tgctctgttt gctgaagcaa taccgccaga gccgccacca attacgatgt
    10561 aatcatatat ttgcattttg ttcctcctta ttgaagagat caatcaaaac ctttatttaa
    10621 cgatcctttt tctacagaaa acaagtgtaa cacttttctt gaattttgta tgcgaagatg
    10681 cttaaaacag ggatatatca gctttttcct tatttacgtt ttcggatcat atctccaggt
    10741 ttcaccggct gtgcgactgg catagttaaa atcatcattg gatttggtgc tcggtcaatg
    10801 gattcgcctt cttcattatg cataacttca acggtttgac tgaaatgact gaatcctgga
    10861 ccataaaatt cgatttcatc tcctacagaa aacaagtttc tctgacgtat cgtagcaatc
    10921 tgtgttgcct catcataaga taatacttca ccgacaaatt tatatactgg gattttacga
    10981 cgttcaccga ataattgttc gttttcagtt ggtgtattgt agtaaaaacc tgtagctaat
    11041 tctcgttgtg ctactttcca taactcgtcg atccattctt gcttgcagac atagttttca
    11101 ggatcttcca tataactgtc tacagccgct ttatagacat ttgacaccgt agatacataa
    11161 tggatcgatt tcattcttcc ttcgatcttg aagctgtcta cgccgttttc aatcaattct
    11221 ggaatatgtt cgatcattga catatccact gcactcatcg agaattcttc ttccacttcg
    11281 cctgtatcag ttaaactgtt tcgttcttga ccaaaaggca tatcgaacaa tccatacttc
    11341 cagcgacaag attgagaaca tccgccacgg tttgcatccc gcatcgacat atggttggac
    11401 aatgtgcatc gaccagaata agagatacac atggcaccat ggataaatgc ttcgatttcg
    11461 gtatccgtat ttttacgaat ctctgccact tcctccattg aaacttcacg agctaaaacg
    11521 acacgttcca acccttcttc tttccagaat tccaatgtct caaaattagt tgctgaagct
    11581 tgagtggata aatggatcgg cagacctggg gcttctgtcg cacaaatttc gatcaaagct
    11641 ggatcagaaa caatcactgc agaaataccg acatcacgca aactgcggaa aaattcccct
    11701 gctccatctt catttccttc atgggtgacc atattagcag caacgtatac cttcgcatca
    11761 tgttcttttg caaaagcaac tccttcagcc atttcttcat aagtaaaatt acctgcacgg
    11821 cttcttaaac catatgcatt cccgccaata tatactgcat ctgctccata atggatcgcg
    11881 gtttttaact tttccagtgt tcctgctggc gctaggattt ctggtcgttt caattttctt
    11941 tttactgtca tttctttcat catactcctt atttgatagc agaaggatca atataaaaga
    12001 atcctgtatc taattcacgg ttttcaggat gcaatttact gatttcgcta gtcatttttt
    12061 ccgctttttc ttgatcccag ctaccttcta aaatttgttt ttttgcttcg gcgaatagtc
    12121 gagtgatgtt tacgaatttt tcgcctggag taaataatcc gtccaatttc catgtacagt
    12181 aatgatgctg ataaagttca ttcaattcat tcgagagatt cagatcattg ctggcaaaaa
    12241 tatgtgtgcc atgactatct tcataaatag aataatgtgt ctcttctttt ttaggttccg
    12301 agataaaaag acctctttca cgatcttttt gttcgtcttg tttcgtatag ttatagtaat
    12361 tctgtagcag tggacgtttg gattgatgga tgcatgtagc accatacaca agggtttcta
    12421 caggaatatc gagtttttct tccatcgcct tcatctcttc aaaaggaact tcacgagcaa
    12481 gtactgctcc aactgcgcct tttttcgccc agaaattgat ttgacgtgcg ctcgttacta
    12541 atgtttcagc atcataaata tacggtatac gcaattcagg attttttttc atcgtataaa
    12601 taatacctgt atcaccaact gtgatcacat ctacgtttat ttcttttaaa aaagccaaat
    12661 attcaggtac atttttcatt ttttcaggat gaatgatccc atttacagca atattcgctt
    12721 ttttaccata cttgtgtgct aattctacca attgtcgctg ttcttctcgt gtaaatgaag
    12781 caggcagacg caatccaaat tgctcttcac cgaaatatag tgtatctgca cctgcagcaa
    12841 gcaactgttc tgcttgttca atagtttcta ctgtggttat gagttcgatc ataattttct
    12901 cctcctacaa cttcataata gtagcatagc tctaaaaact ttccaatact tcttctattt
    12961 aaagtcggat ttttgaaaat tatttcacaa aaaatcaaaa taaagttcga tatattctgt
    13021 ccaaatataa aaagagattg tgacaaaaat cttgtcacaa cctcttaatc aagtcttact
    13081 attataaaaa ttcagctaca caggccaagt aactataggg aacctactgt ttccttttta
    13141 ggtccgcgaa cattcaatgt gatcttacct tttgaagcac ctactgttac gtggtcacct
    13201 aaacgaattt ctccgccaag caaagcctcg ctcaatcgat cttcaatttc tttttgaagt
    13261 gcgcgacgga ttggacgtgc accatattca ggatcaaatc cagctttccc gatcacatcg
    13321 attgcagatg gtgtgatttt cacttgaata tcttgttctt tcaatcgttt gataatcgct
    13381 ttgctcatga tttggacgat tgtatggatt tcatctttac tcaatgaatg gaagacgaca
    13441 gtctcatcga ttcgattcaa gaattcaggt cggaaagctt tctttaactc ttctaagata
    13501 cgtttttgca tggctttgtg atctttagta acatcttgga cattgaatcc gacatttttt
    13561 tcttctcgaa tttgtgtcgc tccgatattt gaagtcataa tcatgattgt atttctaaag
    13621 tccacttttc tacctttaga atctgttaaa tgaccatcat ccaatacttg cagaagtaaa
    13681 ttgaaaacgt ctggatgtgc tttctccact tcatctaaga gaatcactga atatggtttt
    13741 tgacgaattt tttctgtcaa ctgtccacct tcatcatacc ctacgtatcc tggaggagac
    13801 ccgattaatc ggcttgtact gtatttttcc ataaattctg acatatcaac acggatcagg
    13861 gcatcttcac ttccaaacat gacttctgaa agtgctttgg ccaattctgt ttttccgaca
    13921 ccagttggtc caaggaacat gaatgaacca attggacgat caggatcttt caatccgctt
    13981 cgtgcacggc ggatcgcacg tgaaactgct ttgacagcct catcttgacc aacgacacgt
    14041 tcatgcagaa gtccttccag ttctaacaag cgttcgcttt cttttttctc caactgctgt
    14101 agagggacac cagtccactc cgatacaact gtagcaacat cttcttctgt tacacgatca
    14161 gaataacctt gctcctgttt tgcttcaatt gcagaaactt tttgcagctt tttacgtagt
    14221 gctttttctt gtctgcgtaa ttgagcagca ttttcaaagt cttgtttttg gatcgctgct
    14281 tccttttctt gtactagttc atcgatttct agtttgatca cagttgattt tgttaagtga
    14341 tcggtttgat ccaaacggac tttcgctgct gattcatcga tcaaatcaat tgctttatcg
    14401 ggtagttgac gagaagtgat ataacgaaca gaaagattga ctgctgctcg tacagcttca
    14461 tctgtgatct ccactccatg gtgctcttca tatcttgtac gtaatccttg aagaatcact
    14521 tctgcttctt caggagttgg ctcatctact tgaatgcgag caaaacgtcg ctctaatgca
    14581 gaatcttttt cgatatattt ttgatattca tctagggtcg ttgctccgat cgtttgtaat
    14641 tctcctcgag ctaacgccgg tttcaagata ttcgaagcat caattgctcc ttctgctcca
    14701 cctgcaccaa ttaaggtatg caattcatca atgaataaga ttacttgacc atcattatag
    14761 atttcgtcga tgaccttttt cattcgatct tcaaattcac cgcgatattt cgtaccagca
    14821 accaaagatc ccatatcaag catcatcagg cgcttttctt gcatgtcttc tggcacttca
    14881 cctaaaatga ttttttgagc taacccttca gcaatggctg ttttgcctac accaggttca
    14941 cccaccagta cagggttatt ctttgtccgt cggctaagaa tctggataag ccgtttcact
    15001 tctctcgaac gaccaacaac tgggtccagc ttgttttctt ttgctaattt cgtcaaatca
    15061 cgtgctaatg aatctagcgt aggtgttcct tgcggtgttt gccgtttatc ttgtacattt
    15121 acatttctac gacgattcat tccttttcca gcagaatcac cagctagccc cattttttgt
    15181 ttcagaactt gacgggcttt tgaaaggctg atacctaagt tgatcatgat tcgagtagcc
    15241 atgatacttt catcttttaa taagctcatc aaaatatgtt ctgttccaac tttttgagaa
    15301 ccagttttct tcacttcgct gctagctaaa gcaaaaactt gtttcattct tggtgaatat
    15361 ggaagaaaga ctcctttagg gtaagaacgg ataccaccat aaccaatcag atgttcaatt
    15421 tcttcataaa tttccgtttc attcaaatcc atttcacgta atacttttcc agcaattcca
    15481 ttttgctcga ccactaaggc tagtaaaaca tgttctgaac taactgattg atgtttcaac
    15541 cgttttgctt cttcttgtgc aatttctaat actgcttggg ctctttcagt aaaaagttct
    15601 tccatctaat ctcacttccc atcttcataa cttaaacgtt ccagaaatgc tcggagaatc
    15661 cgagcacgtg tatgatcttc atattcattg aaattcaatg tgttttttgc aattgcactc
    15721 aacatcaagt ttccttcttt ttttgtaatt atttggtctt cataaagttt ttgaataata
    15781 gcaaaagcat ttttctcact gattgtatca tcaaataact gatttatctg ctcaagtaat
    15841 tgttttttat ctgaaatcct tacttttgca attcgtatat aaccaccacc accacgtttg
    15901 ctttctacta gataaccacg ttggatggta aaacgggtat taatcacata gttgatttgt
    15961 gaaggaacac agttaaagag gtctgccatt tcaatccttc gaatctcgat catttcacta
    16021 ttttccagaa ttttctttat atatgattca attaaatccg aggtattctg atgtgccatc
    16081 ttattcattc cctttgacta atattgacct taattataac gaatttgata atagaaagaa
    16141 agaagaacgc tttttgttaa ataaatcctt tagaatcaac aaaaaaaaat acggaatata
    16201 ctattccgta tc
//
LOCUS       DARBEC010000064        15951 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.066, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000064 DARBEC010000000
VERSION     DARBEC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.066"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..809)
                     /locus_tag="SCL80_001968"
     CDS             complement(387..809)
                     /locus_tag="SCL80_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEG1107823.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCL80_001969"
     CDS             complement(823..2229)
                     /gene="atpD"
                     /locus_tag="SCL80_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEG1107824.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            complement(2252..3154)
                     /locus_tag="SCL80_001970"
     CDS             complement(2252..3154)
                     /locus_tag="SCL80_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEG1107825.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMYQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            complement(3168..4724)
                     /locus_tag="SCL80_001971"
     CDS             complement(3168..4724)
                     /locus_tag="SCL80_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEG1107826.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGNNSST
                     EDKLKSIQNA"
     gene            complement(4747..5289)
                     /locus_tag="SCL80_001972"
     CDS             complement(4747..5289)
                     /locus_tag="SCL80_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEG1107827.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNKAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYAYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMNYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLENMRNQLNR"
     gene            complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCL80_001973"
     CDS             complement(5276..5800)
                     /gene="atpF"
                     /locus_tag="SCL80_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEG1107828.1"
                     /translation="MLNQLAIAEVGNAMLGNVIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCL80_001974"
     CDS             complement(5891..6106)
                     /gene="atpE"
                     /locus_tag="SCL80_001974"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEG1107829.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVVALILVFGV"
     gene            complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCL80_001975"
     CDS             complement(6159..6878)
                     /gene="atpB"
                     /locus_tag="SCL80_001975"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEG1107830.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMVISLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCL80_001976"
                     /pseudo
     CDS             complement(7180..10023)
                     /gene="rnr"
                     /locus_tag="SCL80_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002418328.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
     gene            complement(10026..10781)
                     /locus_tag="SCL80_001977"
     CDS             complement(10026..10781)
                     /locus_tag="SCL80_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1107831.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLRW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCL80_001978"
     CDS             complement(10884..11120)
                     /gene="secG"
                     /locus_tag="SCL80_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEG1107832.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            complement(11238..11516)
                     /locus_tag="SCL80_001979"
     CDS             complement(11238..11516)
                     /locus_tag="SCL80_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEG1107833.1"
                     /translation="MEIKEEKNRLALFNDEQQEIGEMTWSDAGPDIMIIDHTFVDPAY
                     RGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            complement(11629..12597)
                     /locus_tag="SCL80_001980"
     CDS             complement(11629..12597)
                     /locus_tag="SCL80_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1107834.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     regulatory      12812..12856
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            12880..13854
                     /locus_tag="SCL80_001981"
     CDS             12880..13854
                     /locus_tag="SCL80_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HEG1107835.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIAFALKVNPNIGKHMDRFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEGAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     gene            13859..14434
                     /locus_tag="SCL80_001982"
     CDS             13859..14434
                     /locus_tag="SCL80_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEG1107836.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(14501..15640)
                     /locus_tag="SCL80_001983"
     CDS             complement(14501..15640)
                     /locus_tag="SCL80_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEG1107837.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT         4357 a         3443 c         2856 g         5295 t
ORIGIN      
        1 ctgagccaga atgtggttca ttctgactca gatttgattt tgcacaactc atcagtcctt
       61 attgacaaag agcccaaaag tcttgtcgca gtcccttatt ccaaataaac ggtgttcaat
      121 catctgaccc tcggtattag ctcaaaaaac ggcaaaatat gaggaactat tttccatttg
      181 tttctctaat actcaggaca aaacgctgat ttcacaacct ccgatatacg gtgttccaga
      241 agtagcttct tcgaaaaata agctgagatt tcacaaaaat ttgagaagca attttcgtaa
      301 attccttctt atgtcttgaa gctgaccact tctgtcataa cctcttttta ctgttcttaa
      361 acacggataa acatccgcat ctcattttat gagtgcttag atactttgat acgattgatt
      421 gctcggtgca acgcaacagt tgcccgtttc aattcgttcg tatcttgttt tgcttttgct
      481 tcttcgattt ggcgttctgc gcgttgtttt gcacgttccg cacgagatac atcgatatca
      541 cgttcacgtt ccgcactatc agcaacgatt gaaacaacat tatcacgaac ttccatgatt
      601 ccgccattaa cagcgatcca atcgacgtgg gtatctgaat ctgttcgttt tacacgaact
      661 tcatcaatcg tcaatggaac gatgattggt gcatgtttag gcaaaatccc aagctcacca
      721 gctgtcgtac gtgcaacgac gatagctgca tggtgatcat agaccaaacc ggcaggagtt
      781 accacattta ctgttaaata ttgatccatg gggcacccct ttctagtagc ctagtgtttt
      841 cgctttttct acgacgtctt cgattcggcc aacacttcgg aaagcttctt caggaagatc
      901 atcgtatttg ccttccaaga tttcgcggaa tcctttcact gtttcttcta cagggacata
      961 tgaacctgga agaccagtaa attgttcagc cacgttaaag ttttgtgaca agaagaattg
     1021 gactctgcgg gcacgagaaa ctaacacttt ttcttgatcg gataattcat ccatacctaa
     1081 gatagcaatg atatcttgta attcacgata tcgttgcaat acgtgttgga cttctgttgc
     1141 tactttataa tgttcttctc ccacgatttc aggtgctaaa gcacttgaag aagaagccaa
     1201 tggatctacc gctggataaa tcccttgttc tgttaattta cgttccaagt tggttgtcgc
     1261 atccaaatgg gcgaatgctg ttgccggcgc cgggtcagtg tagtcatcgg ctggcacata
     1321 gattgcttgg atcgatgtga tcgaaccttt tttcgtagat gtgatacgtt cttgcaattg
     1381 acccatttct gttgccaatg ttggttgata accaaccgca gaaggcatcc gtcctaacaa
     1441 ggctgatact tcagatccag cttgagtgaa acggaaaatg ttgtcgataa acaacagtac
     1501 atcttgacct tctacatcac ggaaatattc cgcaatcgtc aaaccagtca aagcaacacg
     1561 catacgtgca cctggtggtt cgttcatttg tccgaacacc atggctgttt tttcgataac
     1621 tcctgaatct ttcatttcat aatacaagtc attaccttca cgagtacgtt cacctacacc
     1681 ggtaaataca gaaataccac catgttcttg ggcgatatta tggatcagtt cttggatcag
     1741 tacggtttta ccaacaccgg caccaccgaa tagtccgact ttcccacctt ttaaatatgg
     1801 ggcaagcaaa tcgataactt tgatccctgt ttccaaaatt tctgtacttg tacttaattc
     1861 gtcaaaggct ggtgcttttt tatgaatttc gcttctttcc gcatcttctg ggaaaggtgt
     1921 ttctaaatcg atcgtatctc ctagtacgtt gaatacgcga cctaatgttt ctttacctac
     1981 aggaacagag attgctttgc ctgtatcaat gacttccatt cctctttgta aaccatctgt
     2041 tgattccatc gcaatggtcc ggatcactcc gtcccctaat tctaacgctg cttcaagaac
     2101 aacttttgat ttattttcgt catttttata aacaactaat gcgttgttga tatctggtaa
     2161 ggattggtct aaagaaaatt ccacgtcgac aacgggaccg attacttgaa caatcttgcc
     2221 tgaactcatc ttttttcctc caatctcaat actattctag tgccgaagct ccggcaacaa
     2281 tttcggtaat ttcttgggta atagcccctt gtcttgcacg gttgtaagaa accgtcagct
     2341 catcaatgat attagcagca ttatcagttg ctgttttcat tgctgtcata ccagcagcat
     2401 gttctgctgt tttggcgtca acgatcgcac cgtagatcaa actttcagca tactgaggca
     2461 ataactgaga caagatttct tcttttgaag gttcaaaaat atattcttgt tcgaaagtag
     2521 ttgcttcttc tggatctaag tctgagattg gcagcatttt ttctacacga aactgactag
     2581 ttagcgaatt gatatgatgg ttataacata cgtataattc atcgaatact tcgttttggt
     2641 acatagttgt tgccatattt acgattttgc ggacttcatc aaagcttggc tgatcagata
     2701 gattgcgcag ctcatatgct agattgatac cgcgtgcttt aaagaagtct gcaccagttc
     2761 cgccaattgc gatcatcacg taatcatcgg gtgattggtg gtcttcttct aaaatagaca
     2821 tcgtttgttt caatatcgag ctgttatatc caccgactaa accgccatct gcagtgatga
     2881 cgatataacc cgttttctta acaggacggc tgagcaacat gctgttgtaa ttgatatccc
     2941 ctctaggatt ttctgaggca atatcattta actgagtagc agtcaaatgc gtaacgattt
     3001 ctcttacttt tgttgcataa acctgaaact tctgtgaaga agcttcagat tttgtcaact
     3061 tagaagcaga caccatctgc attgcccgcg tgatttggct cgtttttttc gttgaagcga
     3121 tacgtgtctt gatttcattt aatgaagcac ccattcatct cacctcacta tgcattttga
     3181 atggatttca atttatcttc tgtagaggaa ttatttcctt ttgtagctaa gaacatgtct
     3241 ttatattcct gaatcgcact gttcaacgct tcttcttctg gaagatcttt tgttgtacgg
     3301 attgtttcaa agatatctga atgattggta tccagatatt cgaataattc atgttcgaag
     3361 tctaaaatac tgtctacagg gatactatct aagaatccat gtgtcaaagc atacaaaatc
     3421 actacttgtt tttcaacagc tagcggtgca tgcaattttt gtttcaagat ttctactgta
     3481 cggcgtccac gatttagttt tgcttgtgtt gctgcatcca agtcagaacc gaattgtgta
     3541 aatgcttcta attcacggta acttgctaag tctagacgta aagtcccagc aacttttttc
     3601 attgctttga tttgcgcaga accccctaca cgagatacag atagtccggc atcaaccgct
     3661 ggacgagtac ctgcgtagaa caagtcgctt tctaagaaga tttgtccatc agtgatcgaa
     3721 ataacgtttg ttgggatata agcagagata tctccagctt gtgtttcaac aaatggtaag
     3781 gcagtcattg aaccgccacc taaatcatca cttaattttg ctgcacgttc caataagcgt
     3841 gagtgtaagt agaaaacatc ccctggataa gcttcacgac ctggtggacg gcgaagcagc
     3901 aaggaaagtt cacgataagc aacagcttgt tttgaaagat catcaaaaat gatcaagaca
     3961 tgtttcccgt tgtacatgaa ctcttcaccc attgcagtac cagcatatgg tgcgatataa
     4021 agcaacggcg ctggttgaga cgcaccggca ttcacaacga ttgtgtaatc cattgcgcca
     4081 tattttttca atgtttcaac ttgtgtacga actgtagaat ctttttgtcc gattgctaca
     4141 tagatacaga tcatatcttg acctttttgg ttgatgatcg tatcgatcgc aatggaagtt
     4201 ttccctgttt tacggtcacc gataactaat tcacgttggc cgcgtccgat tggtacaaga
     4261 gcgtcgattg cttttagacc tgtttgcatt ggttcgttaa cagatttacg ttgcataacg
     4321 cctggtgcca ttgcttctac tggacgagct ttgtcagtaa cgatttcacc tagtccgtca
     4381 attggctgac ctagcggatt aactacccgt ccaatcaagg cctctccaac tggaacttcc
     4441 atgatctttc ctgttcgttt tactttatct ccttcacgaa tggactcgaa atcgccaagg
     4501 ataatgatac ctacatcatt tgattctaag ttttgcgcca ttccgtatga gccatttgaa
     4561 aattcaagca actcaccact catcgcgttt tctaaaccat gtgcacgagc gatcccgtca
     4621 cctacataag ttacggtacc gatttcttcg actgaaagca cgttttgata attttcaatt
     4681 tgttctttaa tcaaggcact aatttcttct gctttgatgg ccattcgatt cacctacctc
     4741 tttgtactat ctatttaatt gattacgcat attttctaat tgcgtgcgaa tgcttccgtc
     4801 gattactcga tggttagctt caacaatcgc gccaccaata atagaagaat caacgatttg
     4861 ttttaattcg actgattgat agttcattgt ttttgcaaca ttcttttcta attgtattcg
     4921 ttgttcgtct gaaagtggaa ctgctgttgt gacacttccc aacagcaaac cttgatgttc
     4981 atcatatcga cgttcatatt cttcgatcat gaacaaaaga tcatccattc ggttatacgc
     5041 ataaacaact tctaaaaagt ttttaacgat tccatcatag ccactaacaa gcttgtccat
     5101 gatttctcgt ttttcatgag gttctagacg aacatcactt aacatattac ctaatccagg
     5161 tacttgttca tagatttgac gtaagcttac gagatcttga taaatttctt ctgctttatt
     5221 tgaatcgatc gccagttcaa ataaagcttt cccgtaacgt cttcctactg tatatttatc
     5281 tagtttcatt tgaagaacct agaccttcga tatattgatt gattaatgat tcgtgcattt
     5341 ctggagataa ttctttgttc aagatttttt ccgcgatttg aagagatagt tctgcaacat
     5401 cgtcttttac agagttcagc gctgaatcac gttctacagt gatatcagct tgggctttac
     5461 tttttagacg agctgcttct tcttgagcat ccttcaaaat attttggcgg cttaattctc
     5521 cactttcttt cgcatttttg atgatatcag ctgcatcaga acgagaggct aacaattgtt
     5581 gttcgcgttc ttgttccatt ttcgctgagt tgatgcgaga ttgttctgca gaatccaaat
     5641 cattggcgat cttgtcttca cgttttttca aaatatcgct gattggtccc caagcaaaat
     5701 gcttcaagag agccagtagt atcaaaaatg agccgctgac aacgatgaca tttcctaaca
     5761 tcgcattgcc aacttctgca attgccaatt gattcagcat aggcgagcct tcctttcttt
     5821 gaagtacttc tagttttcat gtaaaaaagc agtaacaaac caaattgttt ctgccttaat
     5881 actgattcaa ttaaacacca aaaactagaa tcaaggcgac aactacccct aagataggaa
     5941 cagcttcgac tagagctacc ccgataaaca ttgttgtacg gaattgacca gacatttcag
     6001 gttggcgagc cattgattcg attgttttag agataacttg accgttacca taaccggctc
     6061 cgattgctgc tcccatgatt gcgattgctg ctgcgatata attcataatt gttttcctcc
     6121 taaattagat aaatagtagt ttgtttttaa aacgaatatt attcttccac ttcaatctta
     6181 tggctcatat aaaccataga taaagtaacg aagacaaatg cttggatgct tccgataaac
     6241 aaggagaatg cgatccagac catctctaat ggaattgcta acggcaatga gaaccagcct
     6301 aagcttatta ccatttttgc aatcaaggtt agcaaaactt cccccgcata aatattccca
     6361 taaagacgta aagcaagtgt taataagttt gtaaattctt caatcagttt catcggaaac
     6421 ataaaactat atggtttcaa gaacgaattt acgaaatatc ctttgaagcc taactttttc
     6481 accccaaata agtgagtaag taagatcatt ataaatgcca aagttaaagt aacaaacggg
     6541 tctgctgtcg ggcttttcca aagtgttgtt tcgttaggta aaacgatttt tgttactaaa
     6601 ccgatgttgt ttgcaacaaa aacaaataaa aacaatgtaa atgccaacaa atggaaattg
     6661 ctcaattctt ttctaggcag attatctgtt acgattcctc gactaaaatc aatgacccat
     6721 tctatcgcat tttgtttgcc tgttggtttc aacttaagat ttcttgtaaa aaagtaagct
     6781 aagaagaaaa caatcaaaca ggtcagtaac accattatac aaactgtacc atcaaaccaa
     6841 attggtccaa tgtggaacgt tagcgagcgt tcatccaatc catttcacct cttttccccg
     6901 aaattcacat aaaatcaatt catgtgcgta tctggttttt tccaatcggt ttctaaccac
     6961 acggtatcat accaccatga aacagaaaat tcaaaacatt ttcatgaaaa ttagacactt
     7021 ttttatttca gtcaaaaacg aagcgaattt ttctttatac acggtgctcc tgatctaaga
     7081 aaaaacctat gataatcatg ttttatcaga ttaaatgcta acaaatttta tcattaagta
     7141 aaagcaaaag caccgaatat gcgaaaaagg ataatgaaat tatctagaga aattttttaa
     7201 tgttcgatca atttgtcgat cgatatcttt tcgtttcaaa tcttcccgtt tatcataaga
     7261 ttttttccct ttcgccagac caatcaatac cttggcatag ccgttacgaa tatagacttt
     7321 taaaggaaca acagtgattc cagtattttt caattcattt tcaagccgga tgatttgttt
     7381 tttgtgtaat aataactttc tcgtgcgcaa cggatcatga ttaaaaatat ttccttgttc
     7441 ataaggactg atatgaacat tatgcaagaa agcttctccg ttgcggacgc gaataaatcc
     7501 atcttttaga ttgatccggc tgtttcgtat cgacttgatc tcggttcctt gcaagaccat
     7561 ccctgcttcc atcgtgtcga tgatcgaata atcatggcga gctttcttgt tttgtgcaat
     7621 taattttccc tcgccttttg gcatagctgt ttcctccaac tcattttatt ttttcttttt
     7681 cttcttttta acacctttgt aaaatggctt cttccctttt ttcttaccga acgtttgctt
     7741 actatcgcta tatcgtttgt tcttcttgcc atttctttct gggcgttttc ctgcttgttt
     7801 tccttcgcgt tttcctgatt ttccatttcg tgtttttggc cgttccaacg ctgtaacctc
     7861 ttctgcggaa acaagttcaa aatctactgc tcgttcatct ggatcagctt tgactacttt
     7921 tactcgcact ttctgtccga ttttaaaagt cagaccagtt ctttcaccta caagagctaa
     7981 atgattttca acaaaatgga agtagtcctg tttcaattca ttgatatgga tcaatccctc
     8041 tactgtattc ggcagttcga taaacaggcc gaatttcaca acagagctga tgatcccatc
     8101 gaattcttcc cccactttgt ccatcatgaa ttctgctttc ttcatggaat ctacttcacg
     8161 ttctgtttct actgcacgac gttccatctt agaactatga tctgcaattt caggaagttc
     8221 cgtagcccac gaactttgat tgctttcact tttatcttca ctgtaagaac ggatcagacg
     8281 atggacgatt agatcgggat agcgtcgaat aggagatgta aagtgtgtgt agtaatctgc
     8341 cgctaagcca taatgcccaa aattgtcttc tgaatagcgt gcttgttgca tgcttcttag
     8401 caacatagta ttgataactg cagcttctgg tttgtcttct actccttcga tcacatgctg
     8461 taaatctttt ggtgtgatcg ttcctttcgt tcctcggacc agaatgccca aagctgaagc
     8521 aaaatcaaag aagcgctgca ttttttcttc ttttggctgt tcatggattc ggtaaataaa
     8581 cggcagattt aatttattat aatgttcagc taccgtttca ttggccgcaa gcatgaaaga
     8641 ttcgatcaag cgttcaccaa ttcctctgct tcttagctgg atatcatttg ggtgtccttc
     8701 agcatcaaca aggatttttg cttcgcgatc ctcaaaagaa atcgcgccac gtttgattcg
     8761 catcgtttca aggattcgat gcaattcacc catttcttgg aacataggta cgagctgagc
     8821 atagcgttgc atcgtttctt cgtcttgatc ttctaagatt tgattgacag ctgtgtaggt
     8881 catccgttct gttgtttgga tcacacttgg gaaaatatca tgagagacta cttgtccatt
     8941 tggatcaatc tccatttcgc aactcatggt cagccttgga acatggggat tcagtgaaca
     9001 gataccattc gataaacgtt gcgggatcat cggaatcact cgatcagtca aatatacact
     9061 tgtccctctt tcatatgctt ctctgtccaa ttgactgcct tgtgtcacat agtaagaaac
     9121 atctgcaatg tgtactccaa gaaaataatt accgttatct aattttttaa ccgtaactgc
     9181 atcatctaga tcttttgcat cttctccatc aatcgtaaca atgagctgat ttcttaaatc
     9241 tctacgtcca gttatatcct tttcgctgat ttgatctgga acttcatctg cttcagacaa
     9301 tacatcatca ggaaatttcg tagggatacc attagccacg acaattgaga ggatatccat
     9361 gccagggtca ttcttatgac caatcacttg cttgattatc ccttctaaac tagtcgcata
     9421 ccctttttca gggtaatggg tcagttcgat cagcacaata cttccgtcaa ctggacgaac
     9481 tccctctgct gctgcatata ctttaaattg gttgagcttt ctatctttcg ggataacaac
     9541 gccgtaaaga tccgtttctt gtatttcttc ttctgaataa gcaacgaatt cccctgctaa
     9601 ctgagtagtc gcccggtctc gaatctcgac tactttcccc tctgctccgc gatccatatc
     9661 tgtatttgct cgcttcatga tatcgattgc tacggtgtct ccatccattg caaaaccagt
     9721 cgcttctttt gggatataga tgtcatcttc ttcgggatca atcgttacaa atccaaatcc
     9781 tcgttcattt gctcgaaatg ttccttcgat caagattggt tttaaaggaa gtttcacttt
     9841 tcctttttta ttgaaaacta tactctgttc tctttccatt tgagcaatcg tttgtactag
     9901 taatttaaaa tcatcgcttt tttggaaccc taaaccttca gcgatttctt ccatagaaaa
     9961 actgcgtttg cgactgcttt ccatgaaaaa gagaattttc tcttttatcg tttcttttgt
    10021 catatttatt cctcattcca aggtaatttt tctaaaaatt ccaagacgtc tttttctaat
    10081 tcgcgtcttg ctgtaccaac tgtaatcaca tgaccacttt caggatacca tcgaagtgtc
    10141 actcgctgac ggctcaactt gcgtgcagtt tcaaatacgc cattagggtc gatcatttca
    10201 tctttcccag cttgagctaa aaatatcggc tgttggatat ctgacaacct actttctacg
    10261 attgctgcct gatcttgtat atccattaat tgttgttcag ctaacgagcg gtaagcaact
    10321 gctttttcat tgatttcttt ctctgattta cctgctgtct tcagcacttg acgtacgtat
    10381 ttttcaaagt tttcaggcac gtttgttttt acaggagaca aaggcgaaca gaaaaaaccg
    10441 ccgccaatca ctgattcttc ttctagtgct cgtacagcaa agatgccgcc cattgataat
    10501 cctaatacgg caatatcaga aaactgctca gactgtaaaa aatggatggc ttttttagtg
    10561 tctgcccacc agctttctgc tttttcttgc aaaatatcat atggctccat tgttccgtgt
    10621 cctttaaaaa gcggcgcata aactgtataa tctgcttttt ccaaaaatcg tgcgagcatt
    10681 cgaacatcgt ttggacttcc tgagtaagcg tgaagcagca aaaccgctcg ctttccatgt
    10741 tttgcataaa ggggttttgg taattgtctc actcgtctca cctctttcgc ctcattttac
    10801 catagtttct ttcataactt cttcttaagc gttctctttg caataaaaaa agccgcccgc
    10861 cgttttgcga gaggcttatt caactacttc gaagataaat atgctaggac aaataataag
    10921 atcatccaga tagctcccat aactgcagtt gatcgctgca tgacagcttc aaagccgcga
    10981 gctttttgtt tgccgaatag ttgatccgca ccgccggtaa atgcactcgc tgcactattt
    11041 tgcttgcttg gctgcatcat aatcgcaatg accataacaa tcgataaaac gatcacgatt
    11101 cccaaaatta aattatacat atcgcagcct ccttcccaaa agatatcgtc tctttaactt
    11161 tatcatagat aaaagtccgt gacaactcta tataaatgga ataacagctg aaagttcaaa
    11221 actttcagct gttattctta tgactgacgt aagacgtctt gatattctgg tttcttttca
    11281 aactcttttt ttgcataagg gcataaagga atgatctttt tcccgtctcg tcttgctaat
    11341 tctactccag tatacactag cttttcagct aacttttgac cacgatatgc tggatctaca
    11401 aatgtatggt caatgatcat gatgtctggg cccgcatctg accatgtcat ttctccgatc
    11461 tcttgttgtt catcattgaa taaagcaaga cgattttttt cttctttgat ttccatatgc
    11521 cgcactcctc aaataactga tattttttta ttcctacctt attttagcag tctcctgatc
    11581 tataaacaat tatttatcct cgaattactt acaaaaaaga agagaaaatt aattcatcgt
    11641 tattctttgt tcaataaatg aaaaaatcac tgtccaatag gttttcggat ctttcatcga
    11701 tgcttccaag tgttctgctt ctggaacaag cagcagtgct ttatcccctg ccgtagcatt
    11761 ttgaatcgtg tacgctgctt ctgtagggac aaaatgatct ctttcaccat gaatgaccaa
    11821 taaaggcaga tggttgctgc caagctgtct cgtagctgat gcttgcttta aggagtaacc
    11881 ggcgtatttc tgggcataat gattcgcata tctcatgacc atttttttag gaaagacagt
    11941 gattttactt aacatatatc ggaattctgc ataaacagaa acatatccgc tgtctaaaat
    12001 aaatcctttg accgctgatg gaagtttttc accacttgcc atcatgatcg tagaagctcc
    12061 cattgatccg ccgtgtaaaa taatggacgc attcggctgt tcatctaaga tgagttggat
    12121 ccaggcaatt aaatctagcc gatctagcca tcccattccg atgatttctc cttcactttc
    12181 tccgtgcgca cgtaaatcag gtacaagaac gttgaatcct ttctctgcat atcttttacc
    12241 tatatgagac atgtctcttt tccctgtgct tcggtaatca tgtacgcaaa tgacccattt
    12301 attctgctta tttggatgga tgaacatctg accagctagt ttcaacccgt cataactttt
    12361 gattgttttg ggtgttccct gctcccaaaa gctttcacac tcttcttcta atttttgtcg
    12421 ttttttttct actgacgtga ctgcagcagg gtccatcaaa cgatgaccag tgtcttcata
    12481 ccagtcatat cctctctgta ctcccgctct tactaatttc tttgcataaa aataagaaag
    12541 aaaatggtaa ctgatcccta taattatcag aataagaatg aaataaaaaa taaccattgt
    12601 acacccgccg ctttctgcca aactataatc taactattat ccaatgtacc gatccatagg
    12661 attctttatt ttcggcacac gtaaattcca tttctttttc attgtatcat ttacttaaag
    12721 ttctcctcaa tagatttact tgtaagagag catttacatt cgcaataaaa gccagtatac
    12781 taatttcgtg cattataatc aagacgctcg tcttggactg gttcggaaac ttcccagaat
    12841 aaaaaactaa gtatctctaa agaaaggaga ttggaaataa tgcgaaaagt aattattgat
    12901 tgcgatccag ggatcgacga cactttagct ttatcattag ctgtcaaatc ccctgatatc
    12961 gaagtagttg ctattaccgt agtttgtggg aatgtccctg cagatatagg tacgcagaat
    13021 gtgctgaaat gcctggaacg ctgtgatcgt ttagacattc ctgtttatca aggaatggaa
    13081 aaacctttga agcaaccttt catcagtgca caagacactc atggtatgga tggtttaggg
    13141 gaaacaaatt tcccgatgat cttgagaaaa caggcagaac ctctccatgc cgttgatttt
    13201 ttggctgatt atttcaaaga aaagacaaat acatcagtca ttgctttggg tcctttgacc
    13261 aatatcgctt ttgctttgaa agtcaaccca aatattggga aacatatgga tcgttttgtc
    13321 tcgatgggtg gaacttacaa aagccatggt aactgttcgc cagttgctga atacaattac
    13381 tggtgtgatc ctgaagcagc ttcttatgta ttcgagaatc tcaaacaaac gatagaaatg
    13441 gttgggttag atgtgacaag agaaatcgtc ttgacaccga ctatcctcga atactgctgt
    13501 caaatgaatc ctgaagaggg agagtatcta aaagcgatca ctcgttttta ttttgacttc
    13561 cactggaaac aagaaagaat tttaggatgt gtcatcaatg atccgttagc tgttgcttat
    13621 tttatcgagg gggccatctg tgaaggtttc aaaagcttca ctgctgtgga aactcaagga
    13681 ataagtcgtg gacaaacatt ggttgatcgt tatgaatttt ggcagaaacc tgctaacagt
    13741 aaaattatga caaaagtaga tactcgtctt tttttccgaa agtttttaac cgttctttta
    13801 aatgcacaag aagaaacgat tatgaaagat ttggaaaggt taaagatggg ctaacaaaat
    13861 gaataaaaac aaaatggcaa ctcgcgtact tacagtgatg gctttgtgta ttgggatcaa
    13921 ttatattgga ggaacgattg ctttatggct tcgtttgcca atctatctcg attctatcgg
    13981 aacgattttt gctggtgcat tgcttgggcc tgtacctgga atgctgacag gattgtccag
    14041 cggtatttta agtggagtga ctacagatat tttttctctg tattattcgc caatccaaat
    14101 tctcacaggc ttgcttgctg gattgatttt gccaagatat ctcggcaaag ataaaaacag
    14161 cttgaaactg ttattactcg ctttgatttt gtcggtacct ggaacgatcg tttcttcgat
    14221 cattaccgtt cagttatttg gcgggatcac ctcctcagga tccagtatga tcgttcagct
    14281 attacatggg ttaggtttaa gtcagacaac aagtgtgatc ttggtacagg caggtacaga
    14341 ctaccttgat cgtttgttgt ctatcgtcgt tgtttcatta gtcatcacta tgttgccaca
    14401 acgcttgata tattcaaaaa gaaaaatagg ctgaataaac agagctggat tagcaaaata
    14461 atgctaatct ggctctgttt tttcatttat taaaaaaatc ttacgctaag taggataaaa
    14521 taattccttg atatttttca atcatatcca aataattatc gatttcaaca tactcatcta
    14581 cttgatgagg caaagtaacg acaccaggcc caaagacgac aaagtcgaag gaatggttag
    14641 ctttcgtaaa ttcggctgca tccgttgttg ctgccgctcc aactaaaggt aatggctggc
    14701 tgaattgttg ctgaatgcaa tgaatcaaag gagaatctgg atcagctttg acaggtattt
    14761 tgttgtaatc gatcatcaat tccaaatgat agtctgtctc ttgattcaat tcattgacga
    14821 ttgattgcag caatgcaatg attttatcat ttgggtattc tggaatcgat cgaatgttcc
    14881 cttggagctg cgcatggctt gggatgctgt tgacttgatt cccgccactg atcaatgtaa
    14941 cattatggat tgtttttcct aggacaggat tctcgattgt ttcagcgagg tgattcattt
    15001 cagcatttgc tttcgtaatg aattcattta ggtgattgat tgcattataa ccttggtctg
    15061 gcatagagct atgcgcttct tttccgtgcg aggtgactgt atagttgatt gatcccatgt
    15121 gcgtatacat caaactgtaa ttagttggct ctccgatgat caaggcatct aagtcatcta
    15181 catagcctgc tttcgtgagc tgttccccac ctagttcacc cacttcttct ccgacagtgg
    15241 ctaataattt gactgttccg ttaaatggtt ttcctgactc tttcagttcg atcatggcaa
    15301 tcaccattgc tgccaagcca ctcttcatat ctgtagcacc acgtccgtac aaacggttcc
    15361 catggatttc tgctgcaaag ggtgcatagg tccaagacga ttcatcacca gccgccacga
    15421 catccatatg cccagatagg cctaacactt ttcctgattg gcccttttgg taacgagcaa
    15481 tcaaattatc tctaccatct ctgtaagaaa caatctctcc tgttatgtca tgctttgcca
    15541 aaagcttatt caaataggct gctacttcac cttcgtttcc attcactgat ttaattcgga
    15601 tgatctcttg taaaatcgcg attttttctt cttttttcat ctaatcctct cccttgccat
    15661 ttctttttat tgtatttgag tgtgctcgaa ttttcaaata ggagcaacca aagtaaaaat
    15721 gaagaaagag agccacttta tgaaaaatat gctgctactt ccctcctatt ttatttatac
    15781 gagaggactt gctcatagat tcctattcta ctgttctatc cttaaaagct cttatatgat
    15841 gctagttatt gttagctttc ctttttcaag gatgatcttt tcttgtacca ttctttgtaa
    15901 acatcatata cagaagctag ttgcaaaaaa aactctctac ctgctgatat t
//
LOCUS       DARBEC010000065        15440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.067, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000065 DARBEC010000000
VERSION     DARBEC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.067"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCL80_001984"
     CDS             complement(62..1171)
                     /gene="rpoD"
                     /locus_tag="SCL80_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEG1107838.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(1195..3036)
                     /locus_tag="SCL80_001985"
     CDS             complement(1195..3036)
                     /locus_tag="SCL80_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEG1107839.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(3218..5533)
                     /locus_tag="SCL80_001986"
     CDS             complement(3218..5533)
                     /locus_tag="SCL80_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1107840.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(5718..6578)
                     /locus_tag="SCL80_001987"
     CDS             complement(5718..6578)
                     /locus_tag="SCL80_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEG1107841.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(6637..7158)
                     /locus_tag="SCL80_001988"
     CDS             complement(6637..7158)
                     /locus_tag="SCL80_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEG1107842.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            7436..8197
                     /locus_tag="SCL80_001989"
     CDS             7436..8197
                     /locus_tag="SCL80_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1107843.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            8223..10814
                     /gene="mprF"
                     /locus_tag="SCL80_001990"
     CDS             8223..10814
                     /gene="mprF"
                     /locus_tag="SCL80_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEG1107844.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCL80_001991"
     CDS             complement(11002..12189)
                     /gene="tuf"
                     /locus_tag="SCL80_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEG1107845.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            12557..12913
                     /locus_tag="SCL80_001992"
     CDS             12557..12913
                     /locus_tag="SCL80_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1107846.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(13154..14158)
                     /locus_tag="SCL80_001993"
     CDS             complement(13154..14158)
                     /locus_tag="SCL80_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEG1107847.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            14324..15175
                     /locus_tag="SCL80_001994"
     CDS             14324..15175
                     /locus_tag="SCL80_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEG1107848.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            15192..15437
                     /locus_tag="SCL80_001995"
     CDS             15192..15437
                     /locus_tag="SCL80_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107849.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
BASE COUNT         4482 a         3209 c         2731 g         5018 t
ORIGIN      
        1 tttttctttt aaaataaacc aaacagtttt actgatttct cttctggtca atagtccacg
       61 cttattctaa gaaatctttt aattgttttg aacgagaagg atggcgcaat tttcttaatg
      121 ctttagcctc gatctgacga atacgttccc gagtaacacc aaatactttc cctacttctt
      181 ccaatgttct cgtccggcca tcatctaaac caaaacgtaa gcgaaggaca ttctcttccc
      241 gatcagtcaa tgtatctagt acatcttcca attgttcttt tagtaactca taagctgcgt
      301 gttctgctgg acttgttgct tcctggtctt ctatgaaatc acctaagtga gagtcatctt
      361 cttcaccgat tggcgtttca agggaaacag gttcttgcgc gattttcaag atttctcgga
      421 ctttttctgt tggaagatcc atttccgcac cgatttcttc tggcgtaggt tctcttccaa
      481 ggtcttgcag caattgacgt tggatacgta tcaatttatt aattgtttca accatatgaa
      541 ctggaatacg gatcgtacgt gcttggtcag cgattgctcg agtgatggct tgacgaatcc
      601 accatgtagc ataggttgaa aacttaaatc ctttgcggta gtcaaatttt tcaactgcct
      661 tcatcagtcc catgtttcct tcttggatca agtcaaggaa ctgcatgcct cgtcctacat
      721 aacgtttagc gatacttact actaaacgaa ggtttgcttc tgccagacgc tgtttcgctt
      781 cttggtcgcc ttcttcgatt ttcaatgcta acgctacttc ttcttccgca gttaaaaggg
      841 agacacggcc aatttctttc agatacatac gaaccgggtc attgattttt acaccggttg
      901 gcgcagaaag atcttctgtt tttgcttgtt cagctttttt ctcagcactc ttcaagctgt
      961 gaattgatgg atcaccattt tcatccacca cactgatacc tgcgtcttca atcttttgga
     1021 tcaatttgtc catttcatct gcattcaaag tgaatggtgt agcaagctga ttggataaat
     1081 catcatatac aacttgtcgt ttgggtttgt tttctttgat gaaagctgct actgctgctt
     1141 catacgtttt tttatctgtt ccatttgcca taaaagaagg ccccccttga gtagctattt
     1201 ggcttgtttt aactgtttcg ttaaattgat gatttcaatc gtcaattcaa gctcaagctg
     1261 tttgtttcca gttcgtctgg cttcttgctg catttgtttt tttaatgtta tttcatcagc
     1321 taagccagat ttgctgatca catctagcaa gtcttggatt tctcgtggcg aactttcttc
     1381 tgccaccggt aaatattcga tttccaccac caaccgcctt aaattttcat cttgcaaaaa
     1441 gtctaaaaac tctgcaaggt tgaattcata atgttgtgag acatatgcat cgaataaaaa
     1501 ataaacttct gcatatgcat catgaataaa ctgaaattct cgttctttta aaatattacg
     1561 gacagaaact tctttaaatg cacgatataa tagaatctgc tctgcctttt ctacttgcgt
     1621 caaaggtctc tttttccgtg cagtttcttg tctcgtaacc tgagtgtgtt gactttcctg
     1681 aggttcttga cgattttgct gatttgtcct tcgaagatcg cgcagttgtt tttgtatcgt
     1741 ctctctgccg atctgaaact ctgttgacag ttgagataga tacatatctt gttcgattgg
     1801 tgaaacaact cggctaagtt caactagtaa ttcttcgagg tattcgatct tgtctttttc
     1861 attatctaaa tttttccctt gttttaaata acgtgattta aaagtaaaga ctgtttcgcg
     1921 cccatgagcc actaattctt ggaaagatgc ttctccatac ttacggagat attcatcagg
     1981 atcaagcttt tctggaacga ctacgacaga aagtgctaat cgactgtttt gccgaagcaa
     2041 ttcaatccct cgattggttg cttcaaaacc tgcgttgtct ccgtcatagc aaaagaccaa
     2101 ttctttcgtc agcttttcca tttgttggat ctgttgagct gttagacttg tccccataga
     2161 agctaatcca gttttgatac cagcctgcca tgcagcaatc acatccataa acccttcgaa
     2221 caacaaaatt tctccgtttt tccggatatc gcttcttgct ttatcaagat taaataaaac
     2281 ttgtctttta ttgaacagtt ctgtttctgg actattcaag tatttgggct gatctttttc
     2341 ctgccctttt tcaggtgcaa gccagcgtcc tgaaaaaccg atcgtctggc cttgagaatt
     2401 acgaagtgga aacatcactc tttgataaaa acgatcagac atacggccat catcatgcgt
     2461 gacaaacaat ccagagcgtt ctaacaattc atttgaaatt gattcattct tgaatacttg
     2521 ctgaagaaaa gaacgctcat taggtgcaaa acctatgttg aattcttcaa ttaattctaa
     2581 agacaaccca cgactttgca aatattctaa agctggctgt ccagcttttg tatgcaatag
     2641 catgtgatga tagatttccg ctgctttttc atgagcagca atcagtttcc ccgtagcaga
     2701 ttcaggattt tggacagcta tagtacgctg acgccattga tcttctaacg gtatctgttc
     2761 gaagtctgcc acttttacca ctgcttctgg aaaacttaga ccttctagtt cttgaagaaa
     2821 agtaaacacg tttccgcctt taccacaacc aaagcaatga aaaatctgct tgtcttctgc
     2881 aacagaaaaa gacggtgtac gttcttcatg aaaaggacac agccccaagt aatttttacc
     2941 agactttttc aactggacat attgtccaat cacctcaacg atattcgttt gactgcgtac
     3001 tgtttcgata acctcttgag gaatccgttg agccattttt tcaccccctg ctctattgat
     3061 atcttttggg tttaacagaa gatatttcag gagaataacc ggtaaaaaat cgcactaact
     3121 gaaatcagtg cgattttaaa ggacacaatt atacatgttt gattgtatca tatttcattg
     3181 ctttttcaag tataacacat ttctatctca ttaagcatca atttgctact cttttcagta
     3241 actgattttg aatatcagcc ttccagtaca atactcgaag aacaatattt acgagcatga
     3301 atacaaagaa gtatcctacg ctctctccca accctaagtt tacttccaaa attccatgga
     3361 acagcattgc agctacatat atccctacga tcgtcgttac tgctaggaag atgcgcaatg
     3421 ggttgaaggg ataacaagct tttacaactg ccatgcaact gatcccaatc agcaagtaat
     3481 acatcaaagt agtagtttca gcatttcccc atgcgaatgt ttttccaagt agatacaccg
     3541 caatgatatt cactactaca agcaaggcat ttggtaaagc attaataagt gctgtaggca
     3601 aataacggta agtgattttt cttttatctt gttcaaaaga taaaaagaag gaaggatacc
     3661 cttcaatcgc taaatcaatc agcgtgattt ggatagggat aaacggaaat ccaattgctg
     3721 taacggcaca tataatcgat aaaataaatg aataaatcgt tttgataaaa aagatgcctg
     3781 agactttcgt cacattgttg accactcggc gtccttcaaa caggacttct ggcaatgtag
     3841 taaaatcaga gtccaacaat accagattcg caatctgacg tgttgctcca tcaccttctg
     3901 ccatagcaat gctgcaatct gcttctctta acgccaaaac atcattcaca ccgtcgcctg
     3961 tcattgcaac cgttctgccg ctttctttca attcatttac cagcaatttc ttttgctgcg
     4021 gactcactcg tccaaaaacc gtatagcgat gagcagcttc tcgcacttcc atttcttcag
     4081 taatttgaga aagatcgaca tacgcatcat aattcggtaa tcctgcacgt ctagcgatat
     4141 tggagacagt aaaaggattg tctccagaaa tcactttcag atccacacct tcctcatgaa
     4201 gataagctag tgtttcttga gcgttttttc ggattggatc atcgatttca aacagagcaa
     4261 ccggctcaac aaatgctggt ttttcttcgt tcaattgctg atttggagcg atccccaaca
     4321 taagtacgcg atagccattc gcttgggctg tttttagact gtccggaagc cgatcttctt
     4381 ccaccaaacg ttcaggcgct cctacgtaca cgactcccag ttctggaaat tccatcgcgc
     4441 cccatttgcg atcagaagaa aatgcgactt tgtttgtcac atcataccga ttagaaagct
     4501 caaaataatc tctcatagcc cgcatagtga tgttgttatc actgctttct gccagatacg
     4561 tccccataat ttctgggatg gctttctcgt acagcggatg tagtgcttct acccgctgga
     4621 tactcatttt cccttcagta atggtccctg ttttatccaa gcataatgta tcgacatggg
     4681 ccagcgtttc aattgaatac atgtcttgaa caaggattcg tttttttgcc agtttggtta
     4741 cccctgtcgt taatgcaata cttattagca ataccaaacc ctttggaagc atgcctaaca
     4801 aagctgctgc agatgcaata acagaaattt tgatagaagc atcgcggatg aacagtgctt
     4861 ccaaaaatag tatgatcccc aaaggaataa tcacgaaact agtgaatttg gatactttgc
     4921 gaatcgaatc tacaagttca gaacgaatag ctttgtgagt ctttgcctcg ctagtcaagc
     4981 ggacagcata attatctgca cctactcgga taacttcagc catgacagaa ccgcttgtca
     5041 aaaagcttcc agaaagcaat tcagctccag tttctttgac aatcaaatca gattcgcctg
     5101 tcaaaagagc ttcatttgct tctgcccgtc cagatataac ctgtaaatcg gctggtatct
     5161 gttctcctgc tccgattttt accaaatctc ctaataccag ctctttagta gaaatcgatt
     5221 gttctttacc gtcccggatc actagtactt ctgattggga aacaatcgat aatttccgta
     5281 caagatttct tgcatgtatt tcttgataaa ttccaataac gatattgacg acaataattg
     5341 ctaaatacgc catgttggaa tacgcaccta caaataacag acaaatccca ataccaaagt
     5401 tcagaaaatt gaataacgtc aatatattat cgctgaatat ttgttttgta ctttttgatg
     5461 catcttcctg gtagtcattt tgctggccag aagccatttt ctgaactacc tcttgctgtg
     5521 taagtccatt cattttcttt ttctccattt tccgccaact ttcaaaaata atcacgtgtt
     5581 taagtctagc gtgttccaag attaaaaaca acttgttttt ataaaattta tagaaaaaca
     5641 cacgaatgat tttacaaaaa agatgccaac ctttttgttt gaatcacccg tgtgctgttt
     5701 ttattatcaa tctatcttta cttatattgt tcctccaaac ggctcatcca ccaacctagt
     5761 gctgtaccgg ctataaaaag aacaggacaa gctagtaccc cagcaactcc caatcccgcg
     5821 taatcggtag gaatgatcat aatcgttgaa gcaaaaacta cacctaaaat gaaatggaat
     5881 agcttgctat atgcctttga gaaaatgtaa tccatgattt ttgacaatcc aagaacacaa
     5941 agaatcccac caattccgat cggcacaatc acttcaagtc gtacttcttt aaatccgtca
     6001 gccatcgctt tatacaatcc catatacacc aaaaaattag aagggctcaa tcctgggacg
     6061 atcataccca aaccaatcaa tccgccggca atcagccatg ttccaaaatt ctgatcgact
     6121 tgcccgtcaa ataattgagc gccgtataat aaaaacaaat aagccagaac aaaagtaaca
     6181 accatgatga tcacatcaga agtatctctt ccttttttcc ctgcttcttt ccataacgca
     6241 ggaacagttc cagcgataca tccgacaaaa aaccataata tcgttgtttc atacgcacct
     6301 aataaaaaac tgactaaaaa agaaagtaga aagatgcctg tcactccgcc tagaccaaca
     6361 ggcagaaaaa acaaaacatt ttctttgaaa ttttttgtaa ggtgggctaa aaaagaaatc
     6421 agccgctcat acatgccaaa gacagctgct aatgcaccac cgctgactcc tggcaagata
     6481 aatcctgaac cgataaacat tcccttcaca aaccgtaaca tccagtcttt ggttgtgtac
     6541 cccacttctt gattttccat tttttgcctt ttttccacta tttgcgcact ccttctttca
     6601 tttaatttta ttcatgattg aacatttcct gctattttac ttaaagactt tttcataggt
     6661 ctctattatt ttttcattca ttttgacaat atcaggataa aacatgttac ctccattatg
     6721 atagagatag ccgccattgt aaagcaagga ctggatccgc caatatcggt attgtttccc
     6781 ttcctcattt cctaaaagtg ggctcagcac atctttagaa tacgcttctg ctaattctac
     6841 tgtgttttct ccgccatgat tttttacata ggcaatataa tctttcccaa aattatatgc
     6901 ttggacagct gtccataaat cgcatccttg tttctttgct tctactatca tttccgccaa
     6961 atatttgaca ccttgatcaa tactgtcagc tggattatca atgatctcag cttctccatg
     7021 cagactttcc gagctctgca tgagatctgt cccttttcct tttgattcgg tcaatattat
     7081 cgccttgatc aaattctcgt agttttccat atgataattt tttgcagctg ttgttatttc
     7141 tatttgatag ctttccactc gttttaccga acgataagtt cttcctcata aaaacaacac
     7201 cgcaccaata accgtcagaa tgatcatgta aaaaagaata cggataatag ataatcgttt
     7261 tcgcttcatt tcatcaccta gttattttga catctttttt tatcttacta tttctttatc
     7321 ttcttggcta tttcttcctt gatttctgtg tacgccttaa gaaaaaatga attttcctgt
     7381 attttttgac atcttacgaa aatgatcgtt gtttttcagt aaaaacaatt gcccaatgac
     7441 tgatataatc gttatacttt acacagtagg tgattatgtg aaaaaagaaa agaaatatgc
     7501 aaaaatgcca gatgggagcg aaatttacta tgaaaagagc ggtcaaggct tccctctctt
     7561 tttactgcat ggcaatgacg gcagcggccg ctttttttca gaacaggtcc ctgtactcga
     7621 acgctactat accgtgtatc tagtagatag ccgaggacat ggtcgttcga caaacgaagc
     7681 atctatgttg aattttcagt taatggccga ggatctgaat acgatcatgt tgctggaaaa
     7741 aatcgatcag gcagattttc ttggatttag tgatggagca aatctagcac tggtatttgc
     7801 cagcagcttc cctaaaaaag ttcatcgatt gattttaaat tctggaaaca cgcttgttaa
     7861 aggcgttcgt ttttctgcca gagtcatcag taatattcat tatgcatggg tatggttact
     7921 ctctttattt cgcccaagtc ttcgcaaaaa tcttttagtt atcaaacttt tgctgcatga
     7981 catcgggctt acagaaaatg atttaaaaaa aatcaactcc cccactttga tcatcgtggg
     8041 aaaaaaagat gtaatcaagc taaaacattc tttgtatata gctaaaacta taccaaaagc
     8101 ttcctttgtg cttgtgaaag aacaaggaca tgaattagct agaaaagatc ctgaacgctt
     8161 caatcgagaa gttcttcaat ttctttctga aacataacta ttaggaatag aaggtgattt
     8221 atttgttaaa aaataccata caatggctga aaaaccacct aggactgttc aaaacaatct
     8281 ttttgatttc tgtcattgtc attattgtcg gtgaattgat gtccatcggt aaaactctat
     8341 ctatccagca attaggagaa acgtttgcca ctatacctgt atggaaaagc ggactaatgc
     8401 tattgattgg tcttatctct gtgttaccta tggttggtta tgatattatt ttgaatcgga
     8461 tgcttgaaca aaagcagaat cctcgttacc tgtttgagac aagctggctg atcaatacga
     8521 tcaacaatat tgccggtttc ggcgggtttg ttagtgtggg gcttcgctct gaactatatg
     8581 gacaaaagaa agagagcaga aacgtgatcc aagcattatc gaaagttttc ttgtttttga
     8641 tggctggact ttctatttac agtctgcttt cttttttact tgtagtcttc acgccagttg
     8701 ctcctttttt gaagcaatat tggatatggt tgatcggcgg cggtctatat tttcctgtcg
     8761 tctttttatt tactactttc aaaaagaaag gattccttgg tggtacctca cttaaaactc
     8821 aaggacagct attgctagtt tcattgttgg aatggaccgg tgtcctgttt agtttccttt
     8881 ctgttggtta tttaatggaa atccggattg atctgtggca aacaattcca ttattcattg
     8941 ctgcttcagt gataggaatc gtgtctatga ttccaggaga aattgggagt ttcgatgtaa
     9001 tgatgattat cgggttgtcc gctattggtg tgccaagaga aatagtcgtt gtgtggatct
     9061 tgctctatcg cctgttctac tacatcattc cgtttttgat tggaatcgtc tttttcttca
     9121 aaaatatcgg ttccactttt gatcagcgtt attctggtat ccctaaacaa ttagcaacag
     9181 aaattgcgca taaaatcgtg gttgtcttat tatacttttc tggaatcatg ctagtacttt
     9241 ctgccactat cccgcaagct tttacagagt ttcgctggct tcatagtttg aacccattga
     9301 aatttcattt tattatccaa tttcctagta tactattggg atttctttta atcattatgg
     9361 gcagagggat cgcagcacgt gtgaaacgag catatttgcc tacaatcttc ttgattgttc
     9421 tagctttact ttatgtttta ttaagtgatt ttagtttcac accggtggtt ttcttgtcta
     9481 ttctgcttct gatcattctt gcttctaaaa acgaattatt tcgggaacaa ttgatctatt
     9541 catgggagtg gcgtacgatc gatggaatca ttattggtgc actgtccttg ctttacatcg
     9601 ttattggggt atacaatctg ccggatttcc ctcatcggcg tcaccatttt atttcctttt
     9661 tcttatttcc ttctgaaaag atttggtttt cgggattgtt ggccatcatc gctgttagtt
     9721 tcatgatcgt tctatttgtt catttccttc aaggagaaaa gaaacagatt ggcgaagcct
     9781 ttaatgaaga aaaggccctg aagatcctta caacttatgg aggaaactcc gatagtcaat
     9841 tgatcttctt aaaagataag cggatgtttg cctatgaaaa agacggtgaa ccaactgttc
     9901 ttctccaatt cgcttgcttc aataataaat gtattgtaat gggtgatcct tctggtaaga
     9961 aagaagattt tccagaagca atcgaagctt ttatcgaaga aacagatcga ctatgctatt
    10021 taccagtttt ctacgaaaca agcgaagaga ttgttatgat tttgcatgaa ttcggctacg
    10081 atttcattaa gatgggggaa gaagcttatg tagacctgaa tagttttaca acttcaggga
    10141 aaaaaatgaa gggcactcgt gctgtcttga atcggatcga acgagaagga tttacatttg
    10201 atgtcttaca accaccattt tctactgagc aaatgagtac tttcaaaaat atctctgata
    10261 attggttggg ttcacgaaag gaaaaagggt tttctttagg ctttttctct gaagattatc
    10321 tacaaagagc gcctattgca gttgtcaaaa atacccaaga agaaattgtc gcttttgcaa
    10381 cgatcatgcc tacttacacg aacaataaag taggaacgat tgatctgatg cgatatgatt
    10441 ctaaaaaagc accttctggc agtatggatt tcttgttctt gcatttgttt gcctatatga
    10501 aagaagaaca tatccaatgg ttcaatttag ggatggcccc tctttcaaat gtcggaactt
    10561 ccagaaagag ttttttacag gaaaggattg catacttggt ttacgagttt ggttctcatt
    10621 tttattcttt ccatggatta agagaatata aaagcaagta cgctacaaac tgggtctcac
    10681 gttatactct atattctaga gatagttgga ttgcatatgt tatgattgct ttgctgatta
    10741 ttgataatgc gccagttgag cagacacaag gtagaataag tggtttgaaa aaatggatac
    10801 gaagaaagta ttaaaaactg cataccgata gatgaacact ctcatgatga ataaaagaga
    10861 ctgaagtaaa acgaaagttc atgcttctac aatctcttct tatacaattg aagaacaaag
    10921 agcggtccaa acagaaatcc tgtttggacc gctcttttat tttaaacatt tttcattacc
    10981 aaaatattcg agtcgcgttt cttatgcttc gatctctgta acgatacctg aacctactgt
    11041 ccgtccgcct tcacgaattg agaatgtcgt tccgttttca acagcgattg gatggatcaa
    11101 gtcaacatca atcgtcacgt tgtcaccagg catgaccatt tctgtatctt ctggcaatgt
    11161 gattgttcct gtaacatcag ttgtgcggaa gtagaactgt ggacgatagt tgttgaagaa
    11221 tggagtatga cgtccacctt cttcttttgt caacacgtac acttctgctt tgaattttgt
    11281 atgtggagta atggaacctg gtttagcaag tacttgtcca cgttcgatat cgtcacgttg
    11341 gatcccccgt aacaacacgc ctacgttatc cccagcttcc ccgtaatcta acgttttacg
    11401 gaacatttct acacctgtta caactgcttt ttgtgtttca ggtttgatcc ctacgatctc
    11461 tacctcatca ccgacacgaa cagcaccacg atcaatacga cctgaggcaa cagttcctcg
    11521 acctgtaatt gagaagacgt cttccactgg caatagtaat ggtttatctg tatcacgttc
    11581 tggtgttggg atatattcat ctactgtatc catcaattcc ataatagcag cttcagcatc
    11641 tggatcgcct tgcaatgctt ttaatgcgga acctttgatc acaggagtgt catcgcctgg
    11701 gaaaccatat tcgctcaata actcgcgaac ttccatttct accaaatcga tcaattcttc
    11761 atcatcgact aaatcaactt tgttcaagaa aacaatcagg tattttacac caacttggcg
    11821 tgacaataaa atgtgttcac gtgtttgagg catcggaccg tcagttgcag aaacaactaa
    11881 gattgcacca tccatttgtg ctgcccctgt aatcatgttt ttcacatagt ccgcgtgtcc
    11941 tggtgcatca atatgcgcgt agtggcgttt ttctgtttca tattctacgt gtgcggtatt
    12001 gatcgtaatc ccacgttcac gttcttctgg agctgcatca atactagcat agtcttgagg
    12061 atttgctagg ccttttttgc ctaatactgt agtgatcgca gcagttaaag tagttttccc
    12121 atggtcaacg tgtccaatcg taccaatgtt aacgtgtggt ttacttctgt cataatgttc
    12181 ttttgccata atttacaaac ctcctaaata atatagatca ctgaaaaatt tcagctctca
    12241 cctttttatt atagtctttt tttgactaaa tcaaaaagaa tacgtccgac ttactttttg
    12301 taagattcga actaagctta ttatacgtca atattggtca aagtcaacta agagcactcg
    12361 tctgtcttat acgcgaaaaa agtacctttt ataaataaaa aaatgtgttt tttgagaaaa
    12421 acttcaagat agtccttaac tttcttttga agcaataaga acaaaatgag gaaaagttta
    12481 aaattcagct gttgccaaca actttcaata gacgaaatct tctatttcgt taataataag
    12541 gaaagaggtg aagtacatga aaaaattaat tggcgtactt gtcgttttag gcatatttct
    12601 cggcggaagt tttgcagctt atcattattt ttacggcggc gaagcttatt atacaaaaat
    12661 cacgacgaat ggcgaaatgt cagctaccca agcagataac ggagagaaat tcacgactta
    12721 tacctacaag caagcggctt ataataaaga tggtgaaaaa aaacaggtga cgcttcgcga
    12781 agaaagagaa catccattaa agatgaatgc gtatcttaaa ctcaaagtca atcctcgtaa
    12841 aggtgtatta agctgggaag aaataaaggc ttctgaagtg cctaaaaatg cggcagaaaa
    12901 aatcgataat tagtaaatgg tcttaaaatt ttattagata aaaagaggct gggacagaag
    12961 cgtttaactc caagaaataa gaaggagttg cttctgcttc cgccgtttat acgtgtttag
    13021 agcgtgagac aaaagtgttc tttacttttg tctcacgctc tttttttgtt atattcgtct
    13081 tttccgatcc ttctcttgac acaattcatt taactgatca ctggtacttg aacacctaat
    13141 attctagtca tttttataaa cgattcaata cttcatccac atttttgatc atcacggata
    13201 tcgtaccatc cggattattc acaaattcaa tcaaatctgg gttgcgatat acatctactg
    13261 gtacgatcag ttcgatcccg tttgatagct tgaatttttg ttttccaaat tttttctcag
    13321 aaatttcttg tacttctttt acaggaggtg cttctgggac aaatccttgc tcttgtactt
    13381 cttctttaaa agccattttt gccgtgatgt tttccttgaa gactttttcc gcaatcatct
    13441 catgatccaa ccgacctttt tcttctatcg tatcatagac agcttctttc aaatcagcca
    13501 tgatatcaaa ttcttctgtc tcaaattttt ttccaagatg cttggctact ttttggatca
    13561 cttttacatt ttcttctaaa gaaggtgcag gaacactttc gatcactctt ccagaaaaat
    13621 aaagctcttt tttgccagaa aactcatatt ttttttcaat taattcgtag gtccaatcag
    13681 tcaaattcac agcgaatgct tcatctgctt tttgtgtttt cgcccctaga attgctcgat
    13741 tcaaaatcaa gcggttgtcg atccctgctt cgtctgcttc aacaaagtga gtgtaagcct
    13801 cacgataatt gactttcaat aaagataagt acatcactgt atctaattca tacaagacaa
    13861 caaatacatc tgcactaggt gcttcttcac tttcttggta gatatcgaac caccgctgaa
    13921 cgattgcttc acttgcttcc acaaaatttt cttgagcgat tttaagtgat tcaacgatca
    13981 tgctttcttc tgctagttgt ccagtttttg tttgagcaga agagattttt tggatcttct
    14041 tttgaagaaa atcacgaata tattcttttg tcagatccag ctctttttgt gaaaagactg
    14101 gagaaccagt ttctctgtca ataatatgca gaattgcttt ttttaaatag atatccattt
    14161 tctcattccc ttctgtctta gtttacggaa aaaaaatcaa aaataccaga atcaaaatca
    14221 ttttctttta aacaaaaatt tcaagagtag aaacaaaatg gattcttttt ttcgaaaaat
    14281 gcgctacact tatagttgaa cgaataaaaa aggagctgaa agtatgaatt cattaacaag
    14341 cacttatcgt ctatcaaatg gttacgaaat cccggtagta ggattcggca catggcaaac
    14401 acctgatggg gatgtcgctg tttcctctgt taaagaagcg ctggctgcag gttatcgcca
    14461 tatcgatact gcccaaggat acaagaatga agaaagcgtc ggccaagcga ttaaggaaag
    14521 tggaattcct agagaagaga tattcttgac aacaaaatta tggaatgcaa accacagcta
    14581 tgaacttgtg atgtcttctt ttgaagaatc attgaagaag ctccaaacag attatttaga
    14641 tctgtttttg atccactggc caaaccctgt tgcttttcgt gataattggg aacaagcaaa
    14701 tgcagataca tggcgagcat ttgaagaact gtatgaagca ggtaaaatca aagcaatcgg
    14761 tgtgagcaat ttcttacccc accatctaga tacactagca aaaacagcaa aaattatgcc
    14821 gatggtcaat caagttttcc ttgcaccagg cgaattacag acaactgtag ttgagtatgc
    14881 aaaaaaacat gatatgatcc tcgaagcata tagtccatta ggcactggta aaatcttcga
    14941 tgtgcctgaa atgaaacaaa tcgctgaagc acatgacaag acgattgcac aagttgcttt
    15001 gcgttggtcg ctgcaacacg aattccttcc tcttcctaag tcagtgacac caagcagaat
    15061 caaagaaaat actgaattat ttgattttga attaacagaa gaagaaatga aacaaatcga
    15121 tcagttagat ggtgtagtcg gcaaagcaaa agacccagat actacccaat tttaagtcga
    15181 aaggtgattt tatggatcca atcgaacgct tgaattctct ctcggaagaa gttacccaga
    15241 cttttcattc cgattttgtc tttttgatcg atgcagagaa aatccaacat tttcctgcaa
    15301 gaaactggac acatgatcaa atcatcgaag aactgaaaaa acggttcgat cattcgctaa
    15361 tggtcaaacc ctggcacgaa catgaagtca tttattcacc tgaactacct gtttttgcct
    15421 tgataccgaa aaaatagaaa
//
LOCUS       DARBEC010000066        15230 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.068, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000066 DARBEC010000000
VERSION     DARBEC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.068"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            278..787
                     /locus_tag="SCL80_001996"
     CDS             278..787
                     /locus_tag="SCL80_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1107850.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="SCL80_001997"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="SCL80_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1107851.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="SCL80_001998"
     CDS             2322..3302
                     /locus_tag="SCL80_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107852.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="SCL80_001999"
     CDS             3424..4842
                     /locus_tag="SCL80_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEG1107853.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="SCL80_002000"
     CDS             4913..6382
                     /locus_tag="SCL80_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1107854.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="SCL80_002001"
     CDS             6392..6859
                     /locus_tag="SCL80_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1107855.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="SCL80_002002"
     CDS             6895..7767
                     /locus_tag="SCL80_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1107856.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFITSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="SCL80_002003"
     CDS             7848..10382
                     /locus_tag="SCL80_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1107857.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="SCL80_002004"
     CDS             10538..11311
                     /locus_tag="SCL80_002004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1107858.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="SCL80_002005"
     CDS             11304..12095
                     /locus_tag="SCL80_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1107859.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="SCL80_002006"
     CDS             12105..12596
                     /locus_tag="SCL80_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1107860.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="SCL80_002007"
     CDS             12614..13024
                     /locus_tag="SCL80_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEG1107861.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="SCL80_002008"
     CDS             13028..14428
                     /locus_tag="SCL80_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEG1107862.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="SCL80_002009"
     CDS             14451..14825
                     /locus_tag="SCL80_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1107863.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5286 a         2135 c         3046 g         4763 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attactcgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttacatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DARBEC010000067        14923 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.069, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000067 DARBEC010000000
VERSION     DARBEC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14923)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14923)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14923
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.069"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            425..1039
                     /locus_tag="SCL80_002010"
     CDS             425..1039
                     /locus_tag="SCL80_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1107864.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            1812..3551
                     /gene="yfcC"
                     /locus_tag="SCL80_002011"
     CDS             1812..3551
                     /gene="yfcC"
                     /locus_tag="SCL80_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1107865.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            3708..4892
                     /gene="iadA"
                     /locus_tag="SCL80_002012"
     CDS             3708..4892
                     /gene="iadA"
                     /locus_tag="SCL80_002012"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEG1107866.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            5003..5206
                     /locus_tag="SCL80_002013"
     CDS             5003..5206
                     /locus_tag="SCL80_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEG1107867.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(5307..5969)
                     /locus_tag="SCL80_002014"
     CDS             complement(5307..5969)
                     /locus_tag="SCL80_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1107868.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(5982..7484)
                     /locus_tag="SCL80_002015"
     CDS             complement(5982..7484)
                     /locus_tag="SCL80_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107869.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            8032..9711
                     /locus_tag="SCL80_002016"
     CDS             8032..9711
                     /locus_tag="SCL80_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEG1107870.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            9838..10317
                     /locus_tag="SCL80_002017"
     CDS             9838..10317
                     /locus_tag="SCL80_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEG1107871.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            10536..11216
                     /locus_tag="SCL80_002018"
     CDS             10536..11216
                     /locus_tag="SCL80_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEG1107872.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            11224..12555
                     /locus_tag="SCL80_002019"
     CDS             11224..12555
                     /locus_tag="SCL80_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEG1107873.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            12570..13532
                     /locus_tag="SCL80_002020"
     CDS             12570..13532
                     /locus_tag="SCL80_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEG1107874.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            13643..13981
                     /locus_tag="SCL80_002021"
     CDS             13643..13981
                     /locus_tag="SCL80_002021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEG1107875.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            14060..14767
                     /locus_tag="SCL80_002022"
     CDS             14060..14767
                     /locus_tag="SCL80_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEG1107876.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
BASE COUNT         4787 a         2594 c         3212 g         4330 t
ORIGIN      
        1 accaatgttt ttttgtattt tcttgcttgc acttggataa gagattactt acaataaatt
       61 cagatagaaa cagctgaata aaaaaaggct ccctgaccct tagttggcgc taagaatcag
      121 ggaatgacca gtcacacaca tacaccctga ccgatcattt tgccatagtc aatgcagtgc
      181 atcgacctaa tttattgtac ctaatctagg gtgaaaattc tacccctatg tagatatttt
      241 ccatggtctg cacgttgttt tatttgctat gtcaaggtat tgggcgtatt cgttcaatgc
      301 cttttttgtg tttgaaacag caaagaccac atgtgttact gtctaactac tgttttttta
      361 tgtacttcct tagattaggc agctgtttct attcatgtca agtatagcta ggcgggagag
      421 ataaatgagt ttatgttatt ttgactatta tcaccaattg gaagagaaat taaggataga
      481 agagccgctg atgcttcaag ctggcacttg gatcgcgaaa caaattaatc aaggtggtag
      541 attgcagata tatgctagcc gcacactaag tggtgtagca tttgaatttt gggatcaaac
      601 cccggatctt gtaccaagta tattgattga aaatccagcc ggaggtgtct atgaaacgct
      661 agaaggtgca ggacaggcaa ttattgatca gctttctgtc aaatcagaag acatttttct
      721 tcttctttcg aatgaaggaa gaaacccggc aatcatcgaa ctagctcagt ggatcaaaga
      781 aaatggtcat ccattaatca ttgttactgg atttgatttg tcacgaagta tcaaatcgca
      841 acatagcagt ggtttgcgat tatttgaatt tgctgatttg gttttggaca accacgccaa
      901 tttacatgat gcagtcttga ctttgccaaa tctagatcca gcgatctgcg gaacagcctc
      961 tcttgcaacg atctttttgc tgcagcaagt tttgtatttt acagcaaaac agctagtcca
     1021 ggacacaagt ggaaaatagt tatccacatg ttgacaaagt tccttaaaaa aataatgttc
     1081 cacataaaaa tggagcgttt gaaaaaaaga aaacgattat taatgaggat taacaacgac
     1141 tacttttttt gttttagtta ttttaagatg gatcatcttt gattttaagc ttgttttgtt
     1201 taaagtttgt tatttttata agttgtttca cagttatgag aaatgagtta taagaatatg
     1261 tagtgtcaga aaaataaaaa gtagtggaaa aagagacaag aaagaatcca tatggattct
     1321 ttcttgtctc ttaatacacg ttgatgaaag agtcttcccc aaaactcttt acaaagtaat
     1381 tatagtacat ttgttatcat tattgcaatg aagtttacct gatataacaa aattgtccaa
     1441 caggatacct ttttctaata gaaataaaat acacaaaaat tgaaggatag ataatggata
     1501 tttataagtg tttattattt tttttgcaat gaaattcaat ttttcaatga ttgtaaaatg
     1561 aaaaaaacag gacgagattt tctctaatct aaaaataaaa aaagaatttt aaggaaaaac
     1621 agagaaataa aatacttaat ataaatggaa agagaaaaaa ataatcatac ataggagaaa
     1681 aaaaacaaaa tagaaaaaag tgattttcta ataaattatt catgtggaaa tgtcattttt
     1741 actgtataat tcatttgatt atccattttt tattcatcaa aattactgaa aaaagaaagg
     1801 aagaaataaa tttgaagaaa acaaattctt caggaaaacc gaaacgcgac ttcactaaat
     1861 tatcgacacc ccatacgtat gtcattattt ttggtgtagt catctttgca tggatactga
     1921 cttttgtcgt tccagcagga aagttcagca cgcaagatat tgaatacaaa gatgcaaatg
     1981 gcgaaacaag tactcgtact gttttacgtc aagattcctt ccgttatgct tacgagctgg
     2041 ataagtcgta tgtgtttgat caattagaag aactgcaaga tcatccagca gaacgtgaaa
     2101 aactggatgt tccggaaaaa ggattggaaa aagtcattgc tgacggagaa aaaaatctta
     2161 cgcaggaaaa actagatgaa atctccttga cagatgatgt gctttacgat cagtacggcg
     2221 aaaatatcta tgatacttct aaaaaattgc ataaaacagc caaaatatgg ggaacagatg
     2281 attttggcgg ctttggtttc ttaaattttg tttttgaagg actagtatct ggagataagt
     2341 atggatctgc tgtagggatc gctgcattga tccttgttgt tggaggtgct tttggtatta
     2401 ttatgcgtac cggagcgatc gacgcaggta tctatgcgtt tatcagtaaa actaagggac
     2461 tggagcgttt agctttaccg ctattattct ttgctttttc attcggtggc gctacattcg
     2521 ggatggcaga agaggtaatt ccattctcaa tggtgatggt tccatttgtc attgcgttag
     2581 gatatgactc gatcgtagca gtaaccgtga cttatgtcgc atctcaagtc ggaaatgcga
     2641 cctcttggat gagcccgttt agcgtagcgg tggctcaagg gattgctgga atccccgtgt
     2701 tgtctggtgc gacattccga ttgattatgt gggtagttgt tactgcatta gccgctggtt
     2761 atatgatgat atatgcagaa aaaatccgta aaaaaccaga aagttcgttg acttacaaat
     2821 cagatgatta tttccgttct catatcaaaa agacatctga tgaaaataaa aaattcatgc
     2881 ttgggcataa attgatctta ttagagatgt tagttgtcct tgtctggatc gtttggggcg
     2941 tgactcaaaa aggctattac attccagaaa tcgcttctca attctttgtg atgggattag
     3001 ttgctggaat cattgctgtt cttttcaaat tagatggaat gaaagtcaat gacatcgctt
     3061 cttctttcca atctggtgca gctgatttag ctggtacagc aatcgtagta ggtatggcca
     3121 aagggattct tcttgtcttg ggaggatctg atgcatctgt tccttcagct ttgaatacga
     3181 ttttgcatgg gatcgggacg gcgttgaccg gtgttccagc tgtcattggt gcttgggcga
     3241 tgtacatctt ccaaagtttg ttcaacttgg ttgttacatc aaattcagga caagcagctt
     3301 tgactatgcc aatcatggct ccactggcag atttagtagg tgtatccaga caagtagcag
     3361 ttcttgctta tcagcttggt tcaggtttta tggacgcttt tacacccgta tcagccagct
     3421 tgattggtgt gctaggtgtt gcacggatcg aatgggcaaa atgggcaaga ttccaaatca
     3481 agatgcaagg attcctgttt gttttaggaa ctattttcat catgattgcc attgcaatag
     3541 gtctgcagta gtactggaga cctgtaaata aaaaagtaat ccaaacagta gataaggact
     3601 ccaagaaccg tcattttacg gtaaaatcaa aggagtaaag atcctatcgt ttggattatt
     3661 ttttaagatc ctatcatcag ttatacttaa agaaagagga aaaatgtatg aaaatcattc
     3721 gacaaattga agtgtttgca ccagattact taggaaaaat ggatgtactg attgccggtg
     3781 ggaagatttt ggctgtagaa gaacagctag aaggcggcta cgaaggagtg gaagtagaag
     3841 aactttcagg tgagggaaaa gtactaaccc cgggtttcat tgactgtcat ttccatattt
     3901 taggtggagg aggagaaggc ggttatcaaa atcgaacgcc agaagtcaca ttgagccaat
     3961 tgactacagc tggtgtaacg acagttgtcg gttgtttggg aacagacgga gaaggtagag
     4021 atatgactgc gttgatcagc aaagcgaaag gtctggaagc agaaggaatc tccacttatg
     4081 tatacgaagg ttcgtaccga ttgccggtaa aaacagtaac agattctatc atcaaagact
     4141 ttttgacaat tgataaaatt atcggaatcg gagaaatcgc agtatccgat caccgttctt
     4201 ctcagccaag ttttgaagag ttcgctcgtg cagtagcaga tgctcgtgta ggaggcatgt
     4261 tatccggaaa agctgggatc atcaatgtcc acttaggcgg aggaaaaaga aagctagagc
     4321 tattgacacg aatcgtagaa gaaacagaaa ttccgatcac acaatttttg cctactcatg
     4381 caaatcgcac accagaatta tttgaagctt gcgtggcatt tgccaaacgt ggtggcacga
     4441 ttgacttcac tgctagcgaa gatcctgatt tttgggagaa gacagatggt gaagttcgct
     4501 tcagcaaagc actgaaacga ttgatcgagg aagatgtttc tttagataat ttcacgatga
     4561 cctcagatgg acaaggaagc ttgccttatt ttgatgaaaa caatcatttc ctagggttag
     4621 gagtaggaag tgccaaggca ttgctagtag ggatcaaaga agctgtgcaa aaagaaagta
     4681 tccctttgga aatcgcctta cgagctataa ccagtaatcc agcacgtata ttgaaattgg
     4741 ataaaaaagg aaaaatcgaa attggtgctg atgcagatct gtgtatcttg gataaagaaa
     4801 cgcttgatat tgatacagtt atcgctaaag gagaaataat ggtccaagaa aaagaagtca
     4861 aagtatgggg gactttcgaa aaaagttttt aatcaacagc tctaactatc ttttttagaa
     4921 aacgagcgtt ctgtttgtct gtatctgttg ttgatgttac cctgaaggta gatcaataaa
     4981 caaactaaga ggaggaagca caatggcaga tttaaaagga cgttctaaag atatgaaaga
     5041 taaagtagtc ggtgaaggaa aagaagcata cggcaaagca acagacgaca agagcaaaga
     5101 aacagaaggg aaagcacaat cactaggtgc tgacatcaaa gaaaaagctc gaaatttagg
     5161 cgacgaagtc aaagacaagt tttctcgaaa agacgaaaaa aaataaaaac cacttattac
     5221 taattgaatc aagcaaataa aaaatccgaa ccatatctgc gagacagata tggttcggat
     5281 tttttatttg atcattatcc tgttttttaa acttcgattt tttgaaattt tttatttttt
     5341 agttcaaaga cttcatcaca gctttttgct acttcaatct catgagtcac gatgataata
     5401 cagcgatttt gttcatgggc gatttcttgg aaaagtttga cgatatcttg tgaagttgtt
     5461 tcatctagat ttccagtagg ctcatccgct acgattagtt gatgttcaca gcagatagca
     5521 cgaacgatag cgactcgctg ctgctgacct cccgaaagtt tggtcacctg tttttttgct
     5581 aatatttcat caattcctac acgagccagt cgatctaaag caacttgtcg ataattctct
     5641 tgcttggtct tagtgatttc cattgctgct atgacgttgt ctagagccga taaataagga
     5701 agcaagttgt aagcttggaa aataatcgag acatcatttt tacgataact tcttaacccg
     5761 attttgttca gtgaacgatc ttgatagtat acctttcctt cttttggtgc atctagtcca
     5821 gaaataagcg aaagaaaagt cgtttttcca gaaccacttg ctcctaaaat agcgtacatt
     5881 tttccagctt cgaaattcag ttctacatct ttataaagcg catcttgcgg atcttgatac
     5941 caaaatccca attgttctgt ccgtaacatc aaattcctcc tttagtttaa taggattttt
     6001 cttgggttta atcttaatat ccctatagaa gctaaaaaga cagaaaacag actgattcca
     6061 aatgctactc cggctaatag tcccaattgt gcaggtttga cattgatttc caattcgctg
     6121 acttgatcag aaacagcaaa tggattattc gtggttcctt gttgaccatc gcgttgaccg
     6181 ccttgttctt gtctgttacc tggcatttgt ccaccgccag gagcttgctg gttttgtgtc
     6241 gtggtttctg tctgttggtc aagtagctgc tgtcccacgg cattcgcgac gacgtttccg
     6301 ctaagcgaag caactcctaa tgcaagaatc atacaaatag ccacttcggt aaacaattgg
     6361 agaatgattt tacttctgga ttctcctaaa gatagcaaga cgcctaactc atgccgacgc
     6421 tcccgaatgg taatcataat gatcagagta agtatgataa taccggctac agccactagt
     6481 aaaacaacat ttttagaaaa acttgccacg ttgtttaaag gttccaacat tgactgatac
     6541 atggaatcat ttgattgtaa gctaaatgta tctgtgtcga tcagcttctc agcttctttg
     6601 agaaactcat ccattttatc tggatcagat agagtgtaaa tagcagaatc tatcgtgtta
     6661 tcgtcagatg tcccatttag ttcatttgca aaagtgtaag aagtgtaaag tgtattcgac
     6721 ggattcatga agttgaattt catacccata ctgccgcttg tttcgctgga ttcataaatt
     6781 cctttgatcg tcatttcata agtggtatct tcatcttcag tactcgtgat tgtgaaagta
     6841 tctccgactg ccaggtcatt tgtttctgct agagtagaat caatgactac attgtttgtg
     6901 cctttgtcgc tgtcatctat tccttcacca tccgtgattt ttgctgttcc atcagaaaaa
     6961 ttactatttt gtgcagttgc tgacacacca ctgatttgaa agtctgcttg agtcatttgc
     7021 ggattcccgc ccattcctcc agcaggttca ccgcctgcat cagtagtttc tgaactgtct
     7081 tcacttgaat cagaactagt gatggcagtt attccatctt ttgccatagc tgaggcggaa
     7141 ctttcaaagg aataactctt tacattatct aattcagcta ttttttccgc gtccgttaag
     7201 gaaactggtg taagagaaaa actgcctgga tctggacgtt cttcagtggt agaaccgctt
     7261 gaagaagttt gttcctgttt tttaaatgaa gcttctcgat tggcacttaa agtcactgtt
     7321 gctccaactt ctttttgtgc ttgattcgct gcttcttctg cagcgcttcg aatcgtgagg
     7381 ccagctagca caaaaataag aatggctgtg aacacggcaa acaataacaa tgaacgtccc
     7441 cattttactt ttgtactttt ccatgcacgt ttcataaaat tcatattttg catccctcct
     7501 agtagatgca tttgataata gacaacctat cttaaagcta gcttaaatct tatgctgttt
     7561 gcacaccatc aacatgaggt tctgtatata taaaaagtca ctatagttat tgcaaaaaat
     7621 gtggtaaaat gaaaaaagta tgtttcaaac cgacgaagaa gaaatgaaag aggaatagca
     7681 aaagaaatcc tagatgctac agagggattt ttacgtgcat agttgttcta acaattgctg
     7741 atactagcga tttagtttat ttgttgtata ataatgggga acagctgaat acttatataa
     7801 gtattctggc tatagcgttt cgcaaaagat ccattttgtt aatgaagtat atccaaaata
     7861 aaatgcccat ccaactgtca gaacgcggat gtgctggtaa ttttcctgct gattctccat
     7921 aagttgagaa atagtgattt tgcatatgct tcattggaat ggctcatata tttagataaa
     7981 gaagaaaatc gacatcttta aacttggatg tcataagaag aggtgaagtt tgtgagtacc
     8041 attaaaatta tcccattagg cggcgttcgt gaaaatggaa aaaacatgta tgtcgcagaa
     8101 gtgggcgagg atatttttat tttagattgt gggctaaaat atcctgaaaa tgagttgttg
     8161 gggatcgacg tcgtaatccc tgattttacg tatttagaag aaaacattga tcgtgtagct
     8221 ggtgtctttt taacacatgg acatgcagat gcgatcgggg cacttcctta cttgttgtca
     8281 aaaatttctg taccagtatt cgggacagag ttgacgatcg agttagcaaa attgaatgtc
     8341 agtcgtaatg aagcagccaa aggatttaag gatttccatg tggtagatga gcatacagaa
     8401 atagactttg gtgaaacagt agtgagcttt ttccgtacca ctcatacgat accagattca
     8461 gtggggatca atctgaaaac aaaagaaggt agtatcgttt atacgggaga ttttaaattt
     8521 gaccaaacgg ctatcccaat gtatcaaaca gattatgcac gattggcgga aatcggcaaa
     8581 gaaggagtct tagccttact aagtgaatcg gcaaatgcag aaaatcctca gcctgtagct
     8641 tctgaagccc aaatagcaga tgaagtattt gatacgatca aatattggga agggcgtatc
     8701 atcgtcgctt gtgtagcaag taatttgcaa cgagtgcaac aagtattgga tgcagcagaa
     8761 aaagcacaac gaaaagttgt cttgactggt caagatttcg aacgaattat tcgcacagcg
     8821 atgcgtttag aaaaacttca attaccaagt gaagatctgc ttgttacgtt gaaagacatg
     8881 aaaaactatg atccagaaga actgatcatt ttagaaactg gccgaatggg tgaacccatc
     8941 aagtctctgc aaaaaatggc aaacggtaca caccgtaatg tcaagattga agaaggagat
     9001 ttggtttata tcactactac gccaagcatc gcgatggaaa ccatcgtggc gaaaacggaa
     9061 gatatcattt atcgagctgg tggaacagtg aagttgatct ctgagaatat gcgcgtttct
     9121 ggccatgcca atcctaatga cttgcaatta atgatcaatt tgatgaaacc aacatatttt
     9181 gtgccagttc aaggagagta tcgtgaatta gctgctcatg cagatttggc tcatgcagta
     9241 gggatgccat acaaaaatat ctatattact ggacgcggag acgtgctgga atataagaac
     9301 aagcgtatga gtgttgcagg aactacaagt gcagaaaatg taatgattga tggattaggc
     9361 ataggtgata tcggaaacat tgttttacgt gatcgaaaaa tcttgtcaga agacgggatt
     9421 ttcgtagcgg ttgtcactat cagtcggcga gaaaagaaaa tcatttcaaa acctcaaatt
     9481 acttcacggg gattcgttta cgtaaaagct agccgtgatc tgatgaaaga aagcagcaat
     9541 atgattgaaa caatcgttga gaaacattta gaaagcaatg attttgagtg gagcaaatta
     9601 aagcaagaca tccgagatca attaggccgt tacttatttg aacaaacaaa acgccgccca
     9661 gtgatcttgc cagtcattat ggaagctact caacgtcgaa ataaaaaata aacaaaaaga
     9721 gtaagatgaa cctctctata gagtgctcgt cttgctcttt ttttgcgaaa aacaaataat
     9781 ttttgaatac agcatccctc gctttataat aaacgataga ataaagggag tgaatcaatg
     9841 atgagtcaaa caaaagtcga acgttattta gaagaacaaa agatcagcta tcaaccactt
     9901 gattttagtc atgtggtaga agaatctttc acagaagaac tacaaaaaag agggattgat
     9961 ccagctttga tatgtaaaac tctcgtagtg aaaggaaaca aaactggggt gatcatcgca
    10021 ttgattcctt tgaacgatca cttagattac aaaaaaacga gacaagtgac gaaagatcgt
    10081 aaagttggtt ttcctggaat ggattttgta ttaatgcata caggatatcc ccacggagca
    10141 aacaccccta ttggaataaa actcgcacat cctgattatt gttttttagc agacagttct
    10201 ttaaaagaaa aagaagaagt aattgtgtca agcggtgaaa tcgggagaag ccttcggata
    10261 aaagtagccg atttagaaaa gatcgtccat cctatttacg ctgatttaat tagttaaggg
    10321 acgagaagag aagctaagca gagcagagac ctcaaacttg agattatatc tgcttggtac
    10381 ttggtgcatc ctgtattatc tgttacaatt gctttgtaca tactgtacta tcaatccaat
    10441 gaaaatatat ttagcagcta tattttttca ttgatgtgca agtgatgaaa tcgtttttaa
    10501 taaaaaagat acagagaaaa cgcgaggtgt aaaatatgaa aattacgtat catggacatt
    10561 cctgtttaac aattgaaatg gaaaatggcc aacgcgtgat gattgacccg tttattacag
    10621 gaaatccatt gacagactta actgcagaag aagtaaagac agattggttg ctgattactc
    10681 atggtcatag tgaccacata ggagatatga ttcctattgc aaaagaaaat caagccacaa
    10741 ttatcagtat tgttgagatt gctgattatg cgaacagcag aggagtagat tcattcggta
    10801 tgaatatcgg tggaaaacat gcgtttccat tcggcgtggt aaaattcgta catgcacagc
    10861 atagttcaag ctatgaagta gacggtatcg ttcagtatat gggcgagcct agcggaatta
    10921 ttattcaagc agaaggaaaa acgatttatc atgcagggga tactgcttat ttcagtgatc
    10981 taggattgct tgcagaagaa ttcgatattg atgtcgcttt cttgccgatt ggcgataact
    11041 atacgatggg gccagaagat gcagcaaagg ctgcgcaaac aattcgtgcc aagaaagtag
    11101 ttccaatcca ttacaatact ttccctgtca ttgaacaaga tcccgaatct tttgtagagt
    11161 tactggaaga tagtgaagga aaaataatga gcgtctcgga aacgctggaa gtgtaggcag
    11221 aaaatggcga caaaacatga tttgatttta gaatatattg aaagtttacc tgttggcaat
    11281 cgaatttctg taagaagcat cgcaaaagaa ttgaatgtca gtgaaggaac agcgtaccgt
    11341 gcaatcaaag atgcagaaaa tgtcggacta gtttcaacaa ttcaacgagt agggaccatc
    11401 cgtatcgaac gcaaattaaa aaaacatatt gaacgtttga catttggcga ggtcgtcagg
    11461 atcattgaag gggacgtttt aggcggggcg gcaggattag acaaggtctt gaataaattc
    11521 gtcatcggcg cgatgactaa acatgccatg gaacgctata ttacgccagg atcactaatg
    11581 atcgtcggca accgggcgga agtccaaaaa ttagcattgg aagatggcgc cgcagtattg
    11641 atcacaggag gattcaatac gactgctgag attgcaaagc tagctgatga tcttgaaatg
    11701 ccgatcttac gcacctctta tgatacattt acagtcgcaa ccatgatcaa ccgagcgtta
    11761 agcgaccaat tgatcaaaaa agatatcatg cttgtcagtg atatctatat gccagctgaa
    11821 aagactcatt atttgcagca gacagatacg atttctgatt atcagcgttt gtcagaagaa
    11881 acgcagcatt cgcgttttcc agtcgtcaac cgccatcatc gtttgatggg gattgttacg
    11941 gcaaaagatg ttttagggaa atcgccaaac cagatcatcg atcgggtgat gacgaaagaa
    12001 ccaatcagtg taaagaaaac aatgagtatt gcctctgtta gccatcaaat gatctgggat
    12061 gggctagaag tcatgccggt cgtttccgat gacttgacat tggaaggctt gattacccgg
    12121 caagatgtaa tgaaagcgat gcagcttgtc cagcgacaac cgcagattgc agatacgatt
    12181 tcggatcaga tatctggaaa tatccatacg attgatactg accgtgaagg aaatcgtttg
    12241 gaaaacccta agttcaaatt cgtagtagca ccacaaatgg tcaatggcgt tggaaccatt
    12301 tcttttggcg tacttagcga aatcatttct gatgtcgcac aaaaaacaat ggtgatgaac
    12361 caaaaacgaa atatcttgat tgaacaagtg aacctacatt atttgcggct gatccaattg
    12421 gaaagcgaat tagacattcg tccacgtgta ttggaaatcg gcagacgttc cgctaaactg
    12481 gatatcgaag tgtttattga aaacaccatc gtttccaaag caatcgttgt ttgtcaagta
    12541 atggaacgtt cttagaaagc aggagtgaaa tgacagtaca gcaagaaata gtagatacaa
    12601 taaaaaaata cgataaaatc attatccaca ggcatttgcg ccctgatcct gatgcattag
    12661 gatcacaagt aggattagcc gaactattac gggaaagttt cccagaaaaa gaaatacgtc
    12721 aagtaggtga aacgattgaa gggcttcgct ttttagcaga aatgcaagaa gttgaagatg
    12781 tcttctacca aggagcgcta gtcatcgtga ctgatacagc taattcaccc agaatcagtg
    12841 atcaaagata tcagttggca gaaaaactga tcaaaatcga ccatcatccc aatgatgacc
    12901 cttatggaga tcttgtgtgg gtaaatacaa aagcaagtag ctgtagtgag atgattgcag
    12961 aatttgcttt gatgtttcct gaatctttaa agatgaacgt taaggctgca cggttacttt
    13021 atgcaggaat cgtcggagat acagggcgct ttttgtatcc ctccacgacg tcccgtacat
    13081 tagagattgc ttcgatattg agaaattatc catttgatgc aagtgctttg aacagagaga
    13141 ttgaacaaat gccgatgaaa gtcgctaaac tttccggtta tctttatcaa aatatccagg
    13201 tggatgaaaa tggtgcagga agaataatcc ttccgcaaga aattcttgat aaatatggaa
    13261 tcgatgattc agagacagct tcgatcgttt cacttcctgg agtgattgac gaagtgctgg
    13321 cttgggggat ttttgttcag cagccttcag ggtattaccg cgtacgttta cgttctaagg
    13381 ggccgatcat caatgaattg gctaaacgcc atcatggagg aggccatcca ttagcaagtg
    13441 gtgcgaatgc aaaagattta gcagaagtag aggaaatcta taaagagatc caagatatat
    13501 gccaatcgta taagagcaga aaaacagaat gatacttcgt tcgaaaacgt gccttttgta
    13561 gtaaaaatca caaatgtatc ttttcaagat cttctttttc cgttacaata gtgctcacta
    13621 aagattttaa aggagaaaga agatgacatt accaaattgc ccagaatgtg gctcagagta
    13681 cacatatgaa gatcgaggct tgttgatttg cccagaatgc gggaacgaat ggaacaaaga
    13741 agaacaagaa actgtcatag aagacggatt agtggtatta gatgcaaatg gaaaccgtct
    13801 gttagagggc gatagcgtga cagtgattaa agacttgaaa gtaaaaggtg cgtcaggtgc
    13861 cattaaacaa ggaacaaaag tcaagaacat ccgtttagta gaaggcgatc ataatatcga
    13921 ttgcaaggta gaaggatttg gaccgatgaa actgaaatcg gaatttgtga aaaagaatta
    13981 aagaatattc aaggtctttt cacattcttt tcctactact aatgataaaa taaagtaaac
    14041 gaaaaggagt gaaacgaaaa tgtatggtta tggaatgtat ggttttttag atccgacatt
    14101 tcttttggtc attcttggat tagttatttc cggaattgcg tcagcgtacg tcaatagtac
    14161 tttccgaaaa tacgatcaag tgagaagcaa aaacaatgtg actggtacac aagcagcaca
    14221 atatatatta caaagtcaac agataaataa tgtcggtgtt caacaaattg ccggagatct
    14281 cacagataac tacaattcag gtaataagat gttgagtttg tctcaagcaa ctgcacagtc
    14341 tacttcagtt gctgcgatag gtgtagcagc tcatgaatgc ggacatgcgg tacaagatgc
    14401 ttcgggttat gtcccattga agattcgtgc agcacttgtt ccagcagcta actttggttc
    14461 gatgatttcg ttccccttga ttatggtagg tgtattgctt agctggaact ctacgttaat
    14521 caatatcggg attttcgctt tttctttagc attacttttc caattggtga cattaccagt
    14581 cgaatttaac gcgtctcggc gagcaatcca aattttgagt gaaggcggac tactgacgga
    14641 agaagaagta ccgatggcaa gacacgtatt atttgcagca gcactgactt atgttgcagc
    14701 agcgctttcg acattcctgc agctgttgcg cttgatctta ttgttcggcg gcaaccgaag
    14761 agattaaaat aaagaagttg tgaaatcagc gctttgatct gagctaatac cgagagttag
    14821 ctgattgaat accatttatt tggaataagg gctctttgtc aataaggact gatgggttgt
    14881 acaaaatcaa atctgggtca gaatgaacct cattctggct cag
//
LOCUS       DARBEC010000068        14815 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.070, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000068 DARBEC010000000
VERSION     DARBEC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14815)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14815)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14815
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.070"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(296..1024)
                     /locus_tag="SCL80_002023"
     CDS             complement(296..1024)
                     /locus_tag="SCL80_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HEG1107877.1"
                     /translation="MLRIAIVDDDCTIVEGIRHYLLKKVPDIIIDHCYTDGDEAFKNL
                     QNQRFDLLITDITMGKISGLELIESLQQTSNRYFTSIVISAHQNFQYAKKGIDLSIKS
                     YITKPIDFPYLVKLVQEEKMRKSAANQNSLTDDSFNESIQQVIDYVQNNYSEKLTLKD
                     VATQFHYHPAYLGRAFKKETGYSFHDYLLDFRIKRASALLKDTFLSIDEISQKVGIDH
                     VSYFSVLFKKTTGHSPSNYRKKLL"
     gene            complement(1002..2576)
                     /locus_tag="SCL80_002024"
     CDS             complement(1002..2576)
                     /locus_tag="SCL80_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1107878.1"
                     /translation="MKNDLSKVIMRFTYILIFFITLLACFTLFFSYINVIEKEKNQNL
                     QNIQQITHYMETYNRILSGIVTEIYQNYDFQENLNDYYLSSPAEYMEDILSKDRSPTY
                     LVDNLKKLVQDDEVKNISISLDYEKYLSAYAPTKNEKISFIRSLSFISEDMPDATLIL
                     DVSINALKKSLFPTHYNYSLSNGIDSVNLSEDISPSKWKKTQQIAIGNHTLQVYENPN
                     YSSQTLLGLAIMIFLLMSLFILAIHQLLNRSFRNYIQQYQEIITSLNHSSTGDYKSIN
                     IENKVGDLKEIAMHINQFYLDNATYQKTLYENNWLKQEAKYAALQYQIEPHFLFNNLE
                     FIRMTAILNDDEEISDSIYLLSKLYRNNTYQPDIIDLKEELINIDLSLQLHQRRYRHN
                     FDYQLIDHIDQLMLPKLSLQPFVENYIKHGFDSSKKENRLTVYATETTNHYVLKIVEN
                     GKGMDQDKLEHIQQKLNDDIPYSENIGISNSFRRIKHLYPGDTRFVIESQLHVGTTIT
                     IKIQKGGNMHVENCNC"
     gene            2805..3743
                     /locus_tag="SCL80_002025"
     CDS             2805..3743
                     /locus_tag="SCL80_002025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107879.1"
                     /translation="MKLKKGLLGNLKKNRALFIMLLPGALWLFIFCYIPMFGVVIAFK
                     DFRISSRGFFTSLFNSPWVGWENFKYLFASQDAWIITRNTVVYNVVGILLSTFCTVSL
                     AIILSQMANKRLLKKMQTFMIFPNFLSWVVISYFVYAFLSTDKGLINILLSNFGVEPI
                     NWYSEPKYWPFIIVFTSLWKGVGYGSIIYYASIMGIDTSYYEAALVDGASKWQQIRNV
                     VLPHLRSIIVIMLILSVGSIFRADFGLFYQLPRNSGALYDVTNVLDTYVYRGLMNSGD
                     IGMSTAAGLYQSSVGFVLILITNKIVNKLDPSSALF"
     gene            3755..4666
                     /locus_tag="SCL80_002026"
     CDS             3755..4666
                     /locus_tag="SCL80_002026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107880.1"
                     /translation="MISKLKRQMYFSLPVERFFTILLILFTLSCIFPFIFVIVISLTD
                     EKALQLNGYELLPAQWSTDAYQYLIQDGGQLLRSLGVTIMITVIGTLITVFMTGTYAY
                     VLSRASFPYRKFFTFYLFFTMLFAGGMVPSYLVMTKMLGLKNTIWALILPLAFSPYNV
                     IILRTFFKKSIPESIIESAKMDGCSEFRVFFQIVLPLAIPGVATIGLFSSLGYWNDWF
                     NALLYIDTNKLIPLQYLLMKIQNSMEFLANNNDITLAQQQAIQNSLPQESTRMAMVVV
                     ATLPIAIVYPFFQKYFVQGLTIGGVKE"
     gene            4690..6162
                     /locus_tag="SCL80_002027"
     CDS             4690..6162
                     /locus_tag="SCL80_002027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEG1107881.1"
                     /translation="MKKNMQKYLIGGASVLTLGLLGACGSSNGEANNETGTTKVTWYQ
                     VGEEPKDFDEVMTKVNEKLKEEINVELDMRLIPDGDYQQKLGVMINSGEEYDICFVNG
                     TDYVNYGNKGAFISLNDHLDKELKDYAAELNEGFIEGGAINGETYGLPVNNDIATTEY
                     WTFNKDLVDQYQLDVENVEGVETLAPVLEKFAEKNTDTNITPIGMTRTSVCIPYDNII
                     GGGVPLAVAFEGDTQTVVNTYETPEMMTYLKAMHEFYKKGYILKDAATVTTDPFRIDS
                     KNWFLGRNSSGYQPLAQKKLEALAGHGLILKPAGKAFKTTGFARVAMHAVSASSKHPE
                     AALKLLNYVNTNQEVADLMVYGIEGKHYDRNEDGSITLTNSDDYYQGAWALVNTYLVS
                     KTSPITEESKKLEEESKKFQNEAPMSPILGFSFDASDFRTEIASVQNIVSQYEVLLSS
                     GTVDPEQYVPELLEKLEKAGLDKIQASAQEQYDEWRKSNK"
     gene            6213..6824
                     /locus_tag="SCL80_002028"
     CDS             6213..6824
                     /locus_tag="SCL80_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEG1107882.1"
                     /translation="MNENKWTKGFNWVFQLLLLNFTYFILICLGFGILGFFPATIALI
                     AGVVNRKKYGLQESVKFMSKIYKKNFVRYNLYCWSATLLLAGNITTIWLCWQQSAPLF
                     YLLSTLLSVITLFLVFAMLNWVVIKSLGLWMMGDRLIDYLLFTATMLPTVVMQGLVLG
                     IYFRILMILPVIFLFGGTAVTVILISKLTERALAKLPCQIQSH"
     gene            6854..8767
                     /locus_tag="SCL80_002029"
     CDS             6854..8767
                     /locus_tag="SCL80_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 20 glycosylhydrolase"
                     /protein_id="HEG1107883.1"
                     /translation="MKLTDKQVANIAFIQQLEIPLTQKDEEQIDLIFTDTKANVRQIK
                     ITKNEQQRICLSYSHEDLLYRGLFICLPRFLAQGGEMPYIEETSIIETSYSLDASRNG
                     VMNKETLKKFISYLALMGYNTLYIYTEDTYEVEDLPYFGYLRGAYTKEEVNEIVEFAS
                     GFGIEVVPSIQTLAHLTQFLKWYAQQELMDDQNTLLIGEEKVYAAIEKMIIACKEMYQ
                     TERIHLGMDEAYQAGLGRYLSLYGYCDRTTLMIQHMDRVLSIVDKYKMKPMIWSDFIY
                     KLLDETGKDRYYNPDFKLKALAAKGLPAGLTYVHWDYGSDEVSSYEKVIDNHLKFCDI
                     DHYVLASGAHIWGRLAPNHGKTEAIVSAAVKASKNKGVRSVMLTTWGDEGQETEHWHS
                     LIGALKLADYVYSEEEWNDFEEIYDRLLGTGSYQLMSELRYFDEVDTITNNNLQMASV
                     SKLLLWQDPLVGIYDFHVAAHNKIATRTIGTYYHELAQKLDMNKNWNPKQPILTTIRQ
                     RYILLAEILAQKAELGIYLQHCCLVEDLAGLMELANRCEALANRVNELASIHEIIWYQ
                     TYKTYGWEVLEQRYAALAHRLKTVARRIKNHLQGQDALEELLEERLPFCQYQVPLEIS
                     GFNYRQSSVSGYN"
     gene            8895..11876
                     /locus_tag="SCL80_002030"
     CDS             8895..11876
                     /locus_tag="SCL80_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 42"
                     /protein_id="HEG1107884.1"
                     /translation="MLSDYYQNLSVISVNTEPLRNYYIPYASKEEAKQELEREKTSQF
                     IDLNGTWDFQYYPSVQQLPENFFQEAFTESYDRISVPGVWQTEGFDLPQYINVSYPIP
                     FDPPYVPNDTPCGVYRRQFQIEQTDARFYLNLEGADSNCFVWINRNFVGYDQVSHSTS
                     EFDVTKYLQVGVNEIQILVMKWCDGTYFEDQDKWRMSGLFRPVYLLQRPTMHLTSYQL
                     RTLTDPKKGSGTLMVSFQRFTPIEVSLPISYELFHPDGRLIAKGECYSEQQINLDVED
                     CLFWTAETPKLYTLLLTCNGEYFKEKVGFRALTIKNRKLLLNGTPIKLYGVNYHDVNQ
                     QGTPVMTREQLRADLLLMKKLNVNTVRTAHYPKSPDFYELADEIGLYVINEADLECHG
                     VVNLYDTEANYQLIANDPTFEKPIMDRVIRSILPYQNRSSIIMWSMGNESGYGVNFEK
                     AQALARKLDDSRLIHNERSIEPVIDQVNDFSNLDVISQMYPPLEDVHRHCQRSDTEKP
                     FFMCEYSHAMGNGPGDLKTYFDLIQQYDSFLGGCVWEWTDHAINRGTLETPSYLYGGD
                     FGEKYHDGNFCVDGLLTPDRQLTVGALEYRNIHSPIQLIAVDEELQEVTLSNITAFTA
                     GSAFNLSLEWYIDGKKTVYEVMNPFILEPGKSCSLPITVPTTIHGAHTVAVSLSVYKN
                     DELYGQQQLFLKKEKMRLPRTAHHNDAPQISVKGTELIIKGESFIYIYQLAKAAFTSV
                     KINQKELLERPTQFSIWRAPTDNDRNLVNKWKAAGYAEMSLEIRKKDWKFVDDHLLIE
                     TSYRGVIPGLQPIFTMHIQLKINGAGVINQKVSVEKNPIFPDFPRFGMDWPLNDTFSK
                     VSYCGYGPYESYPDKHYAAMFGIYEMEIEEFDQPYIKPQEYGNRWQCDWVSIKNHQGS
                     TMTVYGNSNFSALPYSQAQLTEKSHHYELIKENRTWFNLSYKQNGIGSHSCGPYLEDR
                     FRFMEDTFDWNIVITFKGENR"
     gene            11873..13534
                     /locus_tag="SCL80_002031"
     CDS             11873..13534
                     /locus_tag="SCL80_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hyaluronate lyase"
                     /protein_id="HEG1107885.1"
                     /translation="MKETKINQVKKKLSRYQEVVYRNHCLKWSKNKLNVRFNGLFYRF
                     EQLEEVWKPVTNFQSCPVQLINDPKETYDLCITHLYNQHLKNDGFYLKVTNDNQLTIE
                     SANLRGFQYAMEALLESFFSVGDQLLLPELILKHEPSVKIRGIIEGFYGIPWTHEMRL
                     SLFSYMKDNQLNTFMYAPKDDELLRKKWRELYDAQELDKFKELLSAAEASNIDFWYLI
                     SPGNDIDITCEEDIQVLLKKLEQLIELGVFQFGLLMDDIDYQLKGAAKRRFREPAFAH
                     AYLVNRVEEYLSTRLSNHELVICPTEYDNRHGSRYLATLSQEIPEQLPFFWTGPSTLA
                     ASISTEELQEMASVYQRPMLIWDNIPVNDYLEDKELLFMSPYENRTPNLSKERYQVTG
                     VVSNPMAQLEASKFTINSMANYLWNCERFDPLETWTSVVEKVVGSKLQPAYLTLTNAF
                     PNHYTSSLLSDEELLLAKDPEWVTKEMAALVQAVKWIQQEQHTSRLRTELEPWLQAIT
                     ESWTFWQEWQLKKDPKEAEEWLAKKKTHRIGRDLVTAHLEQIIKS"
     gene            13574..14506
                     /locus_tag="SCL80_002032"
     CDS             13574..14506
                     /locus_tag="SCL80_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005229489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEG1107886.1"
                     /translation="MVKRLISANYSDVKKMTSKELKQSIKASEGRVIVCETVANTLPQ
                     SGEITNAEVASAFGADLILLNGFDCYRPAVFGLPNVTIEKVFDLQQTIENPIPKLKEL
                     VGRPVGINLEPIAPKTNMMSEKVEISQGRASSVETIQKAEELGVDFICFTGNPGTGVT
                     NLEITKSVKMAKKYFSGLIIAGKMHSAGSEEPVVNKEAIISYAEAGADILLVPSVGSI
                     PGFLEQDLIEAVRLAKKYDLLTMSAIGTSQESARPETLRQLAIMNKSCGVDLQHIGDA
                     GYGGLAPAENIFETSVAIRGMRHTINCVSRSINR"
BASE COUNT         4815 a         2333 c         2978 g         4689 t
ORIGIN      
        1 atttaaataa ctacatccat ttattctatc ttctaaaata gcctttttca tttcagttaa
       61 atagttcagc gccatttttg cactcgactt gcatagtagt tttttccata ttcctcaacc
      121 tcagcggggg ttgaagacat aaggaaaatc ctatgcgtta tgctcaactg gatctgatta
      181 aatattctaa tgtatttttc tctaccatca ttaaatggtt aatagccaaa aatcaccact
      241 tttcaatggc tccttgttgt ataagtataa ctagaagatg aacacgtctg agaaattata
      301 gaagcttttt acggtaatta ctggggctgt gtccagtagt ttttttgaag agaacagaaa
      361 aatagctcac gtgatctatt cctacttttt gactaatttc atcaatagat aagaatgtgt
      421 ctttcagtag agcacttgct cttttgattc gaaagtctag caagtaatca tgaaaggaat
      481 aacctgtttc ttttttgaag gcccgtccta aataagcggg atggtaatgg aattgtgtag
      541 ctacatcttt gagcgtaagc ttttcactat aattgttttg aacgtaatca atcacttgtt
      601 gaattgactc attaaaagaa tcatcagtta gagagttttg attagctgct gatttacgca
      661 ttttttcttc ttgtactaac ttaactaaat aaggaaaatc aatgggtttc gttatgtaac
      721 ttttaataga taaatcaatc cccttttttg catactgaaa attttggtgg gcactaatga
      781 caatactagt aaagtagcga ttggatgttt gttgcaaaga ttcgattaat tcgagtccag
      841 aaattttccc catcgtaata tctgtgataa gtaaatcaaa gcgctggttt tgcaggtttt
      901 taaatgcttc gtctccgtct gtgtagcagt gatcgataat gatgtcgggg actttcttca
      961 aaagataatg ccttatgcct tctacaattg tacaatcatc gtcaacaatt gcaattctca
     1021 acatgcatat tccccccttt ttgtattttg atagtaatcg ttgttccgac gtgtagttgg
     1081 ctttctataa caaaacgtgt atctccagga tagagatgct ttatacgtcg aaaagaattt
     1141 gagatgccga tattctcact ataaggaata tcatcattaa gtttttgttg aatatgctcc
     1201 agcttgtctt ggtccattcc tttcccattt tcaacgattt ttaacacata atggttggtt
     1261 gtttcagttg catagacagt caaacgattt tccttttttg atgaatcgaa gccgtgttta
     1321 atataatttt caacaaatgg ctgtaaagaa agttttggta acattaattg atcaatatga
     1381 tcaatcagct gatagtcaaa gttatgtcga taacgacgtt gatgtagttg caaggagagg
     1441 tcgatattaa ttaattcctc tttcaaatca attatgtcgg gctgatatgt attattgcga
     1501 tacaacttgc tcaataaata tatactgtcg ctgatttctt cgtcatcatt caatatggca
     1561 gtcattcgaa taaattccaa attattaaat agaaaatgag gctcgatttg atattgaagt
     1621 gctgcatact ttgcttcttg cttcaaccaa ttattttcat aaagtgtttt ttgatatgta
     1681 gcattatcta aataaaactg attgatatgc atagcgattt ctttcaaatc accgactttg
     1741 ttttcgatat tgattgactt gtagtctcct gtagatgaat gatttaagga agtaatgatt
     1801 tcctgatact gctggatata gttacgaaaa cttctattta ataattgatg aatagcaaga
     1861 ataaacaaac tcattaataa gaaaatcatg attgccagac ctaataaggt ttgtgacgaa
     1921 taatttggat tttcatatac ttgtaatgtg tggttaccga tagctatctg ttgcgttttt
     1981 ttccatttag aaggcgagat gtcttcagat aaattgacgg aatcaatacc attagacaaa
     2041 gaataattgt aatgtgttgg aaagagactc ttttttagcg cgttgatcga aacgtctaga
     2101 atcaatgtag catcgggcat gtcctcacta atgaaactta atgaacgaat aaaagaaatt
     2161 ttttcgtttt ttgtaggtgc gtaggcactt aagtattttt cgtaatctaa tgagatagat
     2221 atatttttaa cttcatcatc ttgcacaagt ttttttaagt tatctactaa ataggtaggg
     2281 gagcgatcct ttgacaaaat atcttccatg tattcagcag gtgatgagag gtagtagtca
     2341 ttaaggtttt cttggaaatc ataattttga tagatctctg tcacgatacc ggagagaata
     2401 cgattatatg tttccatata gtgggtgatt tgctgaatgt tttgcaggtt ttgatttttt
     2461 tctttttcaa tcacattgat ataggaaaag aacaatgtga aacaggcaag taaagtaata
     2521 aaaaaaatca atatgtatgt aaaacgcata ataacttttg ataaatcgtt tttcattcaa
     2581 agtcctcatt ttgataatct ctaattttat gaatcattat agcacatatt acaagtgatg
     2641 taaacgcttt gcaaacaggg ctacttatga ttgtgaatac agtttccgtt taaaaactat
     2701 tctattgtag aggtttaata aactaaaaat aatgtataaa aaattaatgt aagcgattaa
     2761 ccaagtgggt ataatcaagg aaacgctgag agaggagaga caaaatgaag ctaaagaaag
     2821 ggttattagg aaatctaaaa aaaaaccgag cattgtttat tatgctttta ccaggtgcgt
     2881 tatggttatt tatattttgc tatataccaa tgtttggagt tgtaatcgct tttaaagatt
     2941 tcagaatttc atcaagggga ttctttacaa gtttgttcaa cagtccttgg gttggctggg
     3001 aaaatttcaa atatctcttt gcttcgcaag atgcttggat cattactcgc aatacggttg
     3061 tttataacgt tgtaggtatt ttgctaagta ctttttgtac agtgtcatta gcaatcattc
     3121 tgagccaaat ggcgaacaaa cggttattga aaaagatgca gacatttatg atttttccta
     3181 attttttatc atgggtagtc atcagttact ttgtttacgc atttttgagt acggataaag
     3241 gattaattaa tattttacta agcaattttg gtgtggaacc cataaattgg tatagtgaac
     3301 cgaagtactg gccttttatt attgttttta cgagtttatg gaaaggcgta ggctatggat
     3361 ccatcattta ttacgcgtca attatgggga tcgatactag ttattatgaa gctgctttag
     3421 ttgatggtgc cagtaaatgg caacagataa ggaatgtagt cttgccacat ttaagatcga
     3481 ttattgtcat tatgttgatt ttgtctgttg gtagtatttt tcgagctgat tttggattgt
     3541 tttatcaatt gccaagaaat tcgggggctc tttatgatgt gacgaatgta ttagatactt
     3601 atgtctaccg tggattaatg aatagcggag atatcggaat gtctacggca gcgggattat
     3661 atcagtcatc agttggcttt gtcttgattt tgattactaa taagatagtc aacaagttag
     3721 atcctagttc agcactattt taggaggaat caaaatgata tcaaaattaa aacggcagat
     3781 gtacttttcg ctgccagtcg aacggttttt cacaatatta ctaatcttat ttactcttag
     3841 ctgtattttt ccctttattt tcgtaatcgt catttcatta acagatgaaa aagcattaca
     3901 actcaacggt tacgaattat tgccggctca atggagcact gatgcatatc aatacttaat
     3961 acaagatggc ggacagttac ttcggtcatt aggcgttaca attatgatta cagtgattgg
     4021 aacgttgatt actgttttta tgacgggaac ctatgcttat gttttatcaa gagcctcttt
     4081 cccttaccgg aagttcttta ctttttatct tttttttacc atgttatttg cgggagggat
     4141 ggttccttct tacttagtga tgacgaagat gcttggctta aaaaatacaa tttgggcttt
     4201 gattctacct cttgctttta gcccatacaa tgtcattatt ttaaggacat tctttaagaa
     4261 gtcgattcca gaatcgatta tcgaatccgc taaaatggat ggctgtagtg agtttcgagt
     4321 ttttttccaa atcgttttgc ctttagcgat cccaggggtt gcaacaatag gtttattttc
     4381 aagcttaggt tattggaatg actggttcaa cgcattgctg tatattgaca ccaacaaatt
     4441 gattccattg caatatttgt taatgaagat tcaaaattca atggaattct tagcaaataa
     4501 taatgacatt acattagcgc agcaacaagc aattcaaaat agccttcctc aagaatcaac
     4561 acgaatggca atggtcgtag tagctacttt accgattgca attgtttatc cgttcttcca
     4621 aaaatacttt gttcaaggat tgacgatcgg tggagtgaaa gaataaaaaa gaataggaga
     4681 tgttaaaaga tgaaaaagaa catgcagaaa tatttgattg gtggtgcttc tgtactaacc
     4741 cttggcctat taggtgcttg tggtagcagt aatggcgaag caaataatga aacaggaacc
     4801 acaaaagtta cttggtatca ggttggagaa gaacccaaag attttgatga ggtcatgaca
     4861 aaagtcaacg aaaaattaaa ggaagaaatc aatgtagagt tggatatgcg gctgattccg
     4921 gacggggatt atcagcaaaa gcttggtgtg atgatcaatt caggagaaga atatgatatt
     4981 tgtttcgtca atggcacaga ttatgtgaat tatgggaaca aaggtgcttt cattagtctc
     5041 aacgatcatt tggataaaga gttgaaagat tatgcagctg aattgaatga aggatttatt
     5101 gaaggtgggg caattaatgg cgaaacttat ggattgccag tcaataacga tattgccaca
     5161 acggaatatt ggacctttaa taaagatttg gtagatcaat accaattgga tgtagagaat
     5221 gttgaaggtg tagaaacatt ggctccagta ttagaaaagt ttgcagaaaa aaatactgat
     5281 acgaatatta cgccaatcgg tatgacccgt accagtgtct gtattcctta tgacaatatt
     5341 atcggtggtg gtgtaccgct agcggttgca tttgaaggcg atacacaaac agttgttaat
     5401 acttatgaga ctccagaaat gatgacttat ttgaaggcta tgcatgagtt ttataaaaaa
     5461 ggatatattt tgaaagatgc agccactgtt acgacagatc catttcgaat tgatagtaaa
     5521 aactggtttt taggtcgtaa ctcaagtggt tatcaaccat tagcacaaaa aaaattagaa
     5581 gctctagcgg gacatggatt aattttaaaa ccagctggaa aagcttttaa aacgactgga
     5641 tttgcaagag tggcaatgca tgcggtatct gcttcaagta aacatccgga agcagccttg
     5701 aaattgttga attatgtgaa taccaatcaa gaagtagcgg atttaatggt ttatggaatt
     5761 gaaggaaaac actatgatcg caatgaagat ggttcgatta ctttaacaaa ctctgatgat
     5821 tattatcaag gcgcttgggc attggtaaat acttatctag tttcaaaaac ttcaccgatt
     5881 acagaagaaa gtaaaaaact agaagaagaa agtaaaaagt ttcaaaatga agcaccgatg
     5941 tctcctattt taggtttctc ttttgacgct tctgattttc gaacagaaat tgcaagtgta
     6001 caaaatatcg tgtcacaata tgaagtactt ttaagctcgg gaacagttga ccctgaacaa
     6061 tatgtaccag agttgttaga aaaactagaa aaagctggat tggataaaat tcaggcatct
     6121 gctcaagaac aatacgatga atggcgaaaa tctaacaaat aattttttac taaatgaaaa
     6181 gaatagacta agcagttagt ggggggattc aaatgaacga aaacaaatgg accaaaggat
     6241 ttaattgggt ttttcaattg ttgttactaa actttactta ttttatacta atttgtttag
     6301 gatttggaat tttaggtttt ttccctgcta cgatagcttt gattgctggc gttgtgaatc
     6361 gaaaaaaata tggcttgcaa gagtctgtga agttcatgag taaaatctat aagaaaaatt
     6421 ttgttagata taatctatat tgctggtcag caactttgtt gttggccggc aatattacta
     6481 cgatctggct ttgttggcag caaagcgcac cattgtttta tttattaagt acattattat
     6541 cggtaattac actgttccta gtctttgcaa tgttgaattg ggtcgtgatc aaaagtcttg
     6601 ggttgtggat gatgggagac cgactgattg actatttgct tttcacagca acgatgttgc
     6661 caacggtagt catgcaaggc ttggtgcttg gaatttattt ccgaatattg atgattttgc
     6721 ctgtgatttt tctgtttggc ggaacagctg taacggtaat attgattagt aaattaactg
     6781 aaagagcgtt agcaaagttg ccttgtcaga tacaatcgca ctaatttttt cacaaaaagg
     6841 agtatttctt tctatgaagt taactgataa acaagtagct aatatagcct ttatccagca
     6901 acttgagatt ccattgacac aaaaagatga agaacaaata gacttgattt ttactgacac
     6961 aaaggctaat gtgcgacaaa tcaagattac taaaaatgag cagcaacgaa tttgcttgtc
     7021 ttattcacat gaagacttgc tgtatcgagg attgtttatc tgccttccac gttttttagc
     7081 tcaaggagga gagatgccat acattgaaga aacctcgatc attgagacca gttattcatt
     7141 agatgcttct cgtaatggcg tgatgaataa agagactcta aaaaaattca tttcttactt
     7201 agcgttgatg gggtacaaca cattgtatat ttacacagaa gatacctatg aagtagaaga
     7261 ccttccatat tttggttacc ttcgcggagc gtatacaaaa gaagaagtga atgaaattgt
     7321 tgaatttgct tctggttttg gcatagaagt agttccatcg atccagaccc ttgctcactt
     7381 gacccaattt ttgaagtggt atgctcaaca agaactgatg gatgatcaaa atactctact
     7441 gatcggtgaa gaaaaggttt atgcggcaat cgaaaagatg attattgcct gcaaagaaat
     7501 gtatcagaca gagcgtattc atttgggaat ggatgaagcc tatcaagcag gtctgggacg
     7561 atatttaagc ctgtatgggt attgtgatcg gacaacttta atgattcaac atatggaccg
     7621 tgtgttatca attgttgata aatataagat gaaaccaatg atttggagtg atttcattta
     7681 taagctatta gatgaaaccg gtaaagatag gtattacaat cctgatttta aactcaaagc
     7741 attagcagct aaaggattac cagcaggatt aacctatgtt cattgggact atggttcaga
     7801 tgaagtttcc tcctatgaga aagtaattga taaccatttg aaattttgtg acattgatca
     7861 ttacgtttta gctagtggtg cccatatctg ggggagacta gccccgaatc atgggaaaac
     7921 agaagcaatt gtttctgcag ctgtaaaagc ttctaaaaat aaaggtgtac gttctgtgat
     7981 gttaactact tggggagatg aagggcagga aacagagcat tggcattcct taattggggc
     8041 attaaaatta gcagattatg tctattctga agaagagtgg aatgattttg aggaaatcta
     8101 cgatcgttta ttaggaacag gtagttatca attaatgtcc gaattacgat atttcgatga
     8161 agtggatacc attacaaata acaacttaca aatggcttcc gtgagtaaac tgctactttg
     8221 gcaagatccg ttagttggta tatatgactt tcatgtagcc gctcataaca aaattgccac
     8281 tagaacaatt ggtacttatt atcatgagtt agctcagaaa ttagatatga ataaaaattg
     8341 gaatccaaaa caaccaattt taacaactat tcgtcaacga tatatacttt tagctgaaat
     8401 tttagcacaa aaggcagaac taggcatcta tttacaacac tgctgtctgg tagaagacct
     8461 tgcaggatta atggaattag cgaatcggtg tgaagcacta gccaatcgag taaatgaatt
     8521 ggcatctatt catgaaataa tttggtatca aacatacaaa acctatggtt gggaagtatt
     8581 agaacaacga tatgctgcat tagctcatcg tctgaaaacc gttgcgcgtc gtattaaaaa
     8641 tcatcttcag gggcaagatg ccctagagga gcttttggaa gaacgattac cgttttgtca
     8701 atatcaagtg ccgcttgaaa tttctggctt taattatcgg caaagctcgg tgtcaggcta
     8761 caattaacga aggaatcttc ccgttgaacg gaccacttgc tcatctaatg ggaaagtggt
     8821 tcgttttcta ttcataagta gcttaccact aactctttta ttaagaagag aaaaaaagta
     8881 ggaagtgaga aaatatgtta tcggactatt atcaaaatct gtcagttata agtgtcaata
     8941 cagaaccatt acgtaattat tatattcctt atgcaagtaa agaagaagca aaacaagagt
     9001 tagaaaggga aaaaacgagc cagtttatcg atctcaatgg tacatgggat tttcagtatt
     9061 atccttctgt ccaacagtta ccggaaaatt tttttcaaga ggcctttact gaaagctatg
     9121 atagaatttc tgttcctgga gtttggcaaa cagagggatt tgacttacct cagtatatca
     9181 atgtatctta tccaattcct tttgatccgc cttatgtacc taatgatacc ccttgtggtg
     9241 tatatcggcg tcagtttcaa attgagcaaa cagatgcacg cttttactta aatctagaag
     9301 gagcagatag taattgtttt gtttggataa atagaaactt tgtaggctat gaccaagtga
     9361 gtcatagtac atctgaattt gatgtgacca agtatttaca ggttggggtt aatgaaattc
     9421 aaattctagt gatgaagtgg tgtgatggta cctactttga agatcaggat aagtggcgaa
     9481 tgagcggtct atttcgtcca gtgtatcttc ttcaacgacc aactatgcat ctgacaagtt
     9541 atcaactacg gacgttgact gatcctaaaa aaggaagcgg aacattaatg gtttcttttc
     9601 aacgatttac tcctattgaa gtatcgttac ctatttctta cgaattattc catcctgatg
     9661 gacgtctcat agcgaaaggg gagtgttact cggaacaaca aataaatttg gatgttgaag
     9721 attgcctttt ttggacagca gaaacgccta aattatatac attactttta acatgtaatg
     9781 gtgaatattt taaagaaaaa gttggctttc gagcactgac gatcaagaat cgtaagttat
     9841 tgctgaacgg aacacctatt aaattatatg gagtcaacta tcatgacgtg aatcagcaag
     9901 gcacgccagt gatgacccga gagcagttac gtgcagattt gttattaatg aaaaaattaa
     9961 atgtgaatac tgtacgaacg gcacattacc ctaaatcacc agatttttat gaattggcgg
    10021 atgagattgg tctgtatgta atcaatgaag cagatttgga gtgtcatggt gtggttaatc
    10081 tatatgatac tgaagcgaat tatcaactaa ttgctaatga tccgacattc gaaaagccga
    10141 ttatggaccg agtgatccgt tctattcttc cttatcaaaa tcgaagctct atcattatgt
    10201 ggtctatggg gaatgaatct ggttatggag taaattttga aaaagctcaa gctttagcac
    10261 ggaaattgga tgattcacgg ttgattcata atgaacgctc gattgaacca gttattgacc
    10321 aagtgaatga ttttagtaac ttggatgtta ttagtcaaat gtatccccca ctggaagatg
    10381 ttcatcgtca ttgtcaaaga agtgatactg aaaaaccttt ttttatgtgt gaatatagcc
    10441 acgctatggg caacggtcct ggagatctta aaacgtactt cgatttgatt caacagtatg
    10501 attcctttct tggcggttgt gtctgggagt ggactgatca tgcaatcaat agaggaactt
    10561 tagaaacgcc atcttattta tacggtggtg attttggtga aaaatatcac gacggcaatt
    10621 tttgcgtaga tggtttattg accccagatc ggcaattaac agtaggtgca ttggagtacc
    10681 gtaatattca ttcgcctatt caactgattg ctgtagatga ggagctgcaa gaagtaacct
    10741 tgagcaatat aactgctttt acagccggtt cagcatttaa cctttctctt gaatggtata
    10801 tagatggaaa aaaaacagtt tatgaagtaa tgaatccttt catacttgag cctggcaaaa
    10861 gttgttcatt gcccatcaca gtacctacaa cgattcatgg tgctcatacg gttgctgttt
    10921 cgctatctgt ttataaaaat gatgagcttt atggacagca acagctcttt cttaaaaaag
    10981 aaaaaatgag attgccaaga actgctcatc ataatgatgc accacaaatt tcagtaaaag
    11041 gcacagagct gataatcaaa ggagaatcct ttatttatat atatcaatta gcaaaagctg
    11101 cgttcacttc tgtaaagatc aatcaaaagg aacttttgga acgtcctact caattttcaa
    11161 tttggcgtgc accaacggat aatgatcgta acttagtaaa caaatggaaa gcagctggat
    11221 atgctgaaat gtctttagaa atcagaaaaa aagactggaa attcgtagat gatcatctcc
    11281 tgatcgaaac gtcttatcga ggagttattc caggattaca gccaattttt acaatgcaca
    11341 tacagttgaa aataaatggt gcaggcgtaa tcaaccaaaa agtatcagtg gagaaaaatc
    11401 cgatttttcc agatttccct cgctttggga tggactggcc gttgaatgat actttttcga
    11461 aggtttctta ttgtggctat gggccgtatg aaagttatcc tgataagcac tatgctgcga
    11521 tgtttggtat ttatgagatg gagattgagg aatttgatca gccatatatc aaaccacaag
    11581 aatatggtaa ccgctggcaa tgtgattggg tatcaatcaa aaatcatcaa ggatctacca
    11641 tgacggttta tggaaactct aatttttctg ctctgcctta tagtcaagct caattaaccg
    11701 aaaagtcaca ccattatgaa ttaataaaag aaaatagaac ttggttcaat ttaagttata
    11761 aacaaaatgg tatcggctca cacagttgcg gtccttattt agaagatcgt ttccgtttta
    11821 tggaagatac ttttgactgg aatatagtga ttactttcaa aggagaaaat agatgaaaga
    11881 aacaaagatc aatcaagtca aaaaaaaatt atctcgttat caagaagttg tttatcgaaa
    11941 ccattgctta aaatggtcaa aaaataagct gaatgttcgt tttaatggat tattttatcg
    12001 tttcgaacag ttagaagaag tctggaaacc ggtaacgaat ttccaatcat gtcctgtaca
    12061 gctcataaat gatccaaaag aaacatacga tttatgtata acacaccttt ataaccagca
    12121 tttgaaaaat gacggatttt atttaaaggt tactaatgac aatcaactta cgattgaatc
    12181 agcaaacctt cgagggtttc aatatgctat ggaagcctta ttagagagtt ttttttcggt
    12241 aggagatcaa ttattattac cggaattgat tctgaaacat gaaccatcag tgaaaattcg
    12301 aggaatcatt gaagggtttt atggtatccc ctggacacat gaaatgcgcc tctctctttt
    12361 ttcatatatg aaagataacc aattgaatac ttttatgtat gcccctaaag atgatgaatt
    12421 attacgtaaa aaatggcgtg aactctacga tgcgcaagaa ttagacaaat ttaaggagct
    12481 tttatctgct gctgaggcta gcaatatcga tttttggtac ttaattagtc ctggaaatga
    12541 tattgatatt acgtgtgaag aagacattca agttcttctg aaaaagttag aacaactgat
    12601 tgagcttggc gtatttcaat ttggtctatt aatggatgat attgactatc aattaaaagg
    12661 tgctgctaaa cgccgttttc gagagccagc attcgctcat gcctatttag tgaatcgagt
    12721 agaggaatat cttagcactc ggttgtccaa tcatgaatta gtcatttgtc ctacagaata
    12781 tgataatcgt catggttctc gctatttagc aacgttaagt caagaaatac cagaacaatt
    12841 accgttcttt tggactggcc caagcacgtt agcggcgtca atttctacag aagagctgca
    12901 ggaaatggct tctgtctatc aacggccgat gctgatttgg gataatattc cggtaaatga
    12961 ttatttagag gataaagaac tattgttcat gagtccatat gagaatcgaa caccgaatct
    13021 ttctaaggag agataccagg ttacaggagt agtttcgaat ccaatggctc aattagaagc
    13081 ttcaaagttt acgatcaata gtatggctaa ttacttatgg aactgtgagc gttttgatcc
    13141 tttggaaact tggacgtcag tcgttgaaaa agtagttgga agtaaactac aaccagctta
    13201 tcttacctta accaatgctt ttccaaatca ttacacaagc agtctgcttt cagatgaaga
    13261 attactgcta gccaaagacc cagaatgggt cacaaaagaa atggctgctt tagtccaagc
    13321 agtaaaatgg atccagcaag aacaacatac atctagattg agaacagaat tggagccttg
    13381 gttacaagca atcacagaaa gttggacttt ctggcaagaa tggcaattaa aaaaagatcc
    13441 aaaagaagca gaagaatggt tggctaagaa aaaaacacac cgcataggac gtgatttagt
    13501 aactgcccat ttagagcaaa ttatcaaaag ttagatatct taaaaaataa aaagcaagaa
    13561 aaaggagaac aaaatggtca agagattaat tagtgcaaac tacagtgatg tcaagaaaat
    13621 gacgtcaaaa gagttgaagc aatccatcaa agcgagtgaa ggacgggtga ttgtttgtga
    13681 aacggttgct aatactcttc cacaatctgg agaaattacg aatgcagaag ttgcgtcagc
    13741 atttggtgca gatttgattc ttttaaatgg tttcgattgt tatcgtcctg cagtttttgg
    13801 actaccaaat gtgacgattg agaaggtttt tgatctacag caaacgattg aaaacccgat
    13861 tcctaaatta aaagaactag tcggtcgtcc tgtgggaatt aatttagaac ccattgcccc
    13921 taaaacaaat atgatgagtg agaaagtaga aatcagccaa ggaagagcaa gctctgttga
    13981 aacgatacaa aaagcagaag aactaggagt cgattttatt tgctttaccg gtaatccagg
    14041 cactggagta accaatttag aaattactaa atccgtaaaa atggcaaaaa agtatttttc
    14101 gggattgatt attgctggga aaatgcatag cgccggctca gaagagccag ttgtgaataa
    14161 agaggcgatt atttcttatg cggaagcagg cgcagatatt ttactagttc cttcagtagg
    14221 gtctatccca ggctttttag agcaagacct aattgaagcg gttcggttgg caaaaaaata
    14281 tgatttgtta acgatgtctg caatcggtac tagtcaggaa agtgcccgac cagaaacatt
    14341 gcggcaatta gccattatga ataaaagttg tggtgttgat ttacaacata ttggagatgc
    14401 tggatacggg ggattggcac cagcagaaaa tattttcgaa acctcggttg caattagagg
    14461 aatgcgacat accatcaact gcgttagccg ctcgatcaat cgctaacaaa attaaataaa
    14521 aaatggttaa attgattatt taagtgggaa catacttgag atatcagcta acttctgtca
    14581 ctttaaaaaa gggactgtga cagaagtgaa aatagacaaa aaatatccga acaagagact
    14641 aggatagcag aaaaactatc gttttgcaac gaggaccaac tggaccgtag gagcaattct
    14701 cttattgttc ggatatttcg cttgttcaag gacttatgtc tgtagtgccc ttttttggta
    14761 ggaaaaatgg tagggaacca tatcattttt aatgaaaatc aagcatttca aaagt
//
LOCUS       DARBEC010000069        14461 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.071, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000069 DARBEC010000000
VERSION     DARBEC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14461
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.071"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(580..1083)
                     /locus_tag="SCL80_002033"
     CDS             complement(580..1083)
                     /locus_tag="SCL80_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEG1107887.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            1442..2467
                     /locus_tag="SCL80_002034"
     CDS             1442..2467
                     /locus_tag="SCL80_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1107888.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            2790..3668
                     /locus_tag="SCL80_002035"
     CDS             2790..3668
                     /locus_tag="SCL80_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEG1107889.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            4144..4260
                     /locus_tag="SCL80_002036"
     CDS             4144..4260
                     /locus_tag="SCL80_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEG1107890.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            5188..5526
                     /locus_tag="SCL80_002037"
     CDS             5188..5526
                     /locus_tag="SCL80_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107891.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            5620..5850
                     /locus_tag="SCL80_002038"
     CDS             5620..5850
                     /locus_tag="SCL80_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107892.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            6250..6621
                     /locus_tag="SCL80_002039"
     CDS             6250..6621
                     /locus_tag="SCL80_002039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1107893.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(6895..7587)
                     /locus_tag="SCL80_002040"
     CDS             complement(6895..7587)
                     /locus_tag="SCL80_002040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1107894.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(7590..8375)
                     /locus_tag="SCL80_002041"
     CDS             complement(7590..8375)
                     /locus_tag="SCL80_002041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEG1107895.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            8612..10012
                     /gene="lacG"
                     /locus_tag="SCL80_002042"
     CDS             8612..10012
                     /gene="lacG"
                     /locus_tag="SCL80_002042"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEG1107896.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            10037..10354
                     /locus_tag="SCL80_002043"
     CDS             10037..10354
                     /locus_tag="SCL80_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEG1107897.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            10367..12040
                     /locus_tag="SCL80_002044"
     CDS             10367..12040
                     /locus_tag="SCL80_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEG1107898.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLGIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            12054..13685
                     /locus_tag="SCL80_002045"
     CDS             12054..13685
                     /locus_tag="SCL80_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEG1107899.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
BASE COUNT         4609 a         2340 c         2935 g         4577 t
ORIGIN      
        1 taaatattaa cgtgttttca aacagtataa aacggtgtgg tttttggaat aaaaaaataa
       61 catatctcat tttacgcttt tgttatttta tacttaaaaa ataacgacac agaaaatacc
      121 gtgtctcaga tttctctact acttctaaaa actactgttt ttttcctttg aaatgccttc
      181 aaaaagtagg atcaggcaga atatactggc agaatcaagg agaagggagc ctaaataagg
      241 agaaaaacca tagaaaatat aaagtaatta cttataaaac aagcttttta tagaaaaata
      301 atatacctga gtagggtata aattaagacg gaattccttt ctagattgag atattgacta
      361 ggaagaatgt taagatttta agcctttaag ctaaaaaaaa accctttttg ttaaataagg
      421 ggaatatagc ctgtcagttt caaactgcaa gtcgtcctgc tgcaataata tagtagatta
      481 gagatgaaaa tagatttttt taaaccaatg actgaataaa aagaccgtca tagatacaca
      541 ttggaatgtg tctattacgg tcttttttta cgaacgattt tattgaagac ggacagttcg
      601 ggtcaaaaat aaaaaagcta cgatcagaaa taagaataaa atgtatccca aaataatggc
      661 tagacggtct aacttatttg tcgatagatg tttgtttaga agttgatagg cccataaacc
      721 tatcagtcct cctgttgtat tagttatcaa atcagtgatg tccgctacgc caataccgaa
      781 taggaattga atcaattctg ccaagaagct tacaaggaaa ataatcccta attttttaga
      841 aaaagaaagt ctcttgaaat tgacattcaa aagcacccca aaaggaaaaa agacaatcaa
      901 gttataagtg gtctcacgta ataccgtcgt attttcaaaa gaaaaagtag aaaaagggac
      961 gagattgaca ttgctgtaag aataagtgag aaattgtgga agattaaatg atagcttgaa
     1021 taaaatcaac cataatagaa agaataaata aatccctaat aaaaattttg atacataccg
     1081 catagacatc tcctccattt aaaaagtata ccatcgaaaa ccgctgaaaa atcataaaaa
     1141 agtatgaagt ttaaggacca acgtagttat cgtacgaaaa ttagaagctt ttttttgttg
     1201 ggaatccggt ttctttaaca aagctaagat agcgagttgc ttggaaaagt tgctatcttt
     1261 tttctgtcaa aacgactcgg aatgttcgtt ttttgttcta atttctccaa gttgtttact
     1321 ttttgatact aagtttatga aaaatcaaga aatagttgtt atactaggta atgctgacaa
     1381 acgggattgg catttcatct tggttcagct ttttttccaa ttactaggaa ggagggatag
     1441 attgttcaag acttattttc gtttcaataa aatacaagat aatccttttc gtatcatgaa
     1501 tctgatgcta cttatcttta ttgtattatg gttaggttac ataaatcatg attttttgat
     1561 ttcaagtttt tcatcattag ggatctttac ttttttctat tatcaaaaca taccgatgaa
     1621 atatttgctg aaacgtatgt ttctgatcgg ttgcggactg ttggttgctt tcgttttggg
     1681 gatcttatcg acttatgttt tatggcttga accatttgcc gtagctttgg ttgctttttc
     1741 cagcagattc gtgttgcgat tattccatat ttctaaacca ggtggcttgt tttttgcgat
     1801 gctttctgcc atgggaacga gcatgcagct gccaatcgct caattaccga tcgtctcact
     1861 ttattttttt atgggtgtcg tttttgccct gattgcggca gtgattacaa aattactgga
     1921 ttcacggcct gagcagacaa ttgaaaaagc aactttaaaa gaaagatttc acgaagagcc
     1981 tttagtgatc attgattctg ttttctacag cgctgcctta tttctttccg tttatgtcag
     2041 tcacggcttg aatctgcata atccttattg gttgaccttg tcatgtgcat cgattttgct
     2101 agcagaaaac ttggatgcga tgaagcatcg acaagtccaa tatctgattg gatcgatggg
     2161 tgggttatgt gtgtcggcat tcttgtcatt tgtgccgttt acgcagttgc agaccatttt
     2221 tctgatcact tttctatatg gaatcgcaca atttctagtt gctagaaatt atgcagttgc
     2281 taatattttt cttaatccga tggcgttgat gctatctaca cttattcgtg gagcatactt
     2341 gatttctttg atcgaatatc gttttttagg gatcgtcata ggaagtttca tcggtttagg
     2401 tgttgcgtgg gtaatgacgg ttggtttgca acattattta acggttgttc gtcaaaaatt
     2461 agaatagaag gacaaatata tgtgccaaaa gaggttatga caagattttt gtcacaacct
     2521 ctaattcaag gtgttcaaaa gccagattct cagcattaag tcaaattcac acaaaaatgt
     2581 ggaaaggaac tttcaaaaaa attttatatt tattctatca aaaaattttg tttatgatgg
     2641 taactgaata aactttcctg agatggtcaa tgctttcctt gaaagtctta cagatattct
     2701 ttcttcatag aaagtttaag ttttatagtt agtgtacaat aaaagtatgt ttataccata
     2761 tggtgtccgg ataatcagtc atttaaacaa tgaggttcaa agatatgaaa aggggaaatt
     2821 tgatgaagaa aataagcaat gaaatccaaa ttctggcaga aactttaact cttcccaaag
     2881 tcgttataga gcaaatgatt gtacaagcaa aaaacatcct tgatgaggtg tttgagcaat
     2941 atagtcatga actaatggat cctaataaag caaaggcgac tgcaaagaaa atagacaaag
     3001 tctatgaaga ggaacctttt gcagcattag ctatttatct atatgcagct tctcaaagtt
     3061 ggaaaaaaat ttatcaacca ttgaagattc caagaactat ctatttggca acaatgaatg
     3121 cttttactag atttattcat gaacatttcc aagtaacagg aagctatcgc tttgaccgtg
     3181 gtttttggat ttggcattac actagtggct tgatctttcg gattggtgaa ttggagtacg
     3241 aaatgacata ttttccgaaa gggtacacgg atctaagatt agaaggaaag cagtgccttt
     3301 ctcttcatat tccttccgat gcagatttat ctcagaataa gataagagat agttacgaaa
     3361 gtgccattcg tttttttgaa ctttattttc ctgattacca ttaccaagtg atgtacacag
     3421 atacttggtt actatcaccc aatcttacta aatggttgaa aaaagagtca aaaatctgtc
     3481 tttttgcagc cgattatcga ttattatcag ttgatgaaca ggacgatagt ggagtccctt
     3541 ggatctttgg acgagtagat gcccaaatac atgattatcc agaaagtacg agtttacagc
     3601 gacaagcaaa agagcaattg cttgcaggcg agcacattgg ctctggctta ggtattctta
     3661 cttcataagt ttagtacttc gaaatagttt aaaagagcgt gagacaaaag taaagaacac
     3721 ttttgtctca cgctctaaac acgtataaac ggcggaagca gaagcaactc cttcggaaat
     3781 aagctgaaat tcacaaaaat ttgaaaagca attttcgtga atttcttctt atttcttgga
     3841 gttaaacgct tctgtcccag cctctttttt agtgttctat cgaacacaca gtacgttcaa
     3901 taggtaaact agctataaca atagatatta aagttaacgg acaagagggc cttttttagg
     3961 tagctaaaac gtaataatga aaatttattt ccagagatag gagtcaatca gctagtctga
     4021 tcgactgttt taacaagttg actttcattt taacttttga taccatttaa aacacaaatc
     4081 gtaattgttt cgatttgtgt tttttagcat gactacaatc aaagaagaaa gtgggtcgtc
     4141 catatggaaa aaacagatat ctcaagtgca taccgccgct tgaaaagccc aaatatcaaa
     4201 acacgaaaac gtgcgctgaa gatcatcaaa gatgtgaaac ggaatagtgg gaaaaggtaa
     4261 gaagaaggaa acttaattaa aaaaatagcc gaaaaacgtt tttcggctat tttcaataat
     4321 cggtttttgt gtaaggtttc cgaaagaact gctgttgatt ttccattttt aacatatgat
     4381 aaattggtag aatctttctt ttcaaattga taccattccc taattccagt tatatagtta
     4441 actacagaac cacaaagcac gtacgtttat aaaagtatta tctgtagtta tttttcaatt
     4501 ccctttcaac ctcttatttt gaccagtagt attgatcact ttacctttta acacctttgt
     4561 tcgtttttga aacattttta gtcaagaatg gaaacgtaac tacataatca aaggttttgc
     4621 gaaatatttc atgaaatatt tcatgtagaa gcgagcaata cggttgcaaa taaccattat
     4681 cgtttaataa gcagttacga aagctcccaa tatctctaac gttcagctca ctccatttct
     4741 tttcttcaaa aaaatcagtg attcgtatct ctttctattt gtcgaaaaag ttaactttaa
     4801 aaagtaacgc cgaaaaaata tgcaaaaaaa taaaaaagca gttactattg ctagaataac
     4861 atcaaggaat aataagtttt ggttttcaga taatagtcga taagttgtgt attggaattt
     4921 tagatacagt aataaagtat ttttcatgaa attttgtgag aatcactatt acaagtatct
     4981 ttaataaaat taatggttaa ggaggaactt aaagtagaat gtaaatgaat agttgtagtc
     5041 agtcgattgt gatactagac tcaaagttgc aggaataagt ttttcaattt gtgattggaa
     5101 gataaaaaat gaaaaagaag ggcttttttt tatactttgt tgattgtagt gtttattttc
     5161 cctctgttta gccaagtaac attttctatg actcaaccag tttcaagtga ggaacatgat
     5221 aacctttttc gtgctacaga agagtttaag aaatatcagg aaaatattgt tccggagact
     5281 ccattagcta gtgagccaat tactgatatt gatggaaacc aaataggatg gtatatacag
     5341 tacgaagtta tggtggtaaa taatgactca atagtaaaaa atacagtagg cttagagcta
     5401 acaccgattg aaaaggtgag ttcattgtta acgtatattt atactgatga aagtgacgag
     5461 actgagatca ttttaacgga ttactcaaaa attgctactg ataaaaagta tatattgtgg
     5521 atgtagacac tagtgaaata ctttatgata tagaaattga tgaaaattta ctagatgtag
     5581 cacaagaaat cgatcatcaa aagcaggaag ctattcgata tgctggtaga aaagggaaaa
     5641 gaaaatattt attacgtgaa tgtagcaaaa gttagagaag atgaaaatga gtggaaagaa
     5701 ttcaagagtc gctactcaat taactcgact ccgacattta cagtttacag agaaggatcg
     5761 atagaaaaaa ctgttttttg gactaaagaa agtggaatgt ctttagctga agtagaagag
     5821 tttttggatt atgttagtat gcaacagtaa gggagttctt attatgaaaa attttgacac
     5881 atattatgtt attgtttttt cgatatcttt gctgttaata ttactaactt taatgcagta
     5941 taaagagtat ccagagtatt ttattttaac ttttgtatat ttagtaagta gctacacact
     6001 tctatataaa ttcagaagtt cttctagagt ttctaaagtt tgtttcttaa ttagcttatt
     6061 gattctataa tatttttgat ttatatagga aattaaaaat attatagaat caacactcag
     6121 agaacgggtt ggtttttaat agatttaata agtactgata atgaaatttc tcctggttgg
     6181 gaaaacgaag ttggttaccc tgtagaagaa ttttttcaag attactttgc ggagtatgcc
     6241 gataggataa tgagagatta cgtaacagat aaaactcaag aagtcatcaa tcaatcccag
     6301 tcattaaatg aattatctga taaggcgaaa gaagaagcag ttgccattat ttcttcaggg
     6361 attagtgact atattgatat gagtggtgaa atcaattatg gcgataaaga caaaattgta
     6421 cgcatgataa aagcttttat caaaactttt gatgtgaagg cagatcttaa gtttaatgat
     6481 acttacctag taggaaaatt aattaatatc cttgggacag agaaagatac ggaaacgttt
     6541 atttcctctt tgtctatgtt gtttacagat aagcattttt caactcagct tggtggagag
     6601 gaactagtgg aaatctttta accattacgc ccagcattaa taaaagtata tcaaaaaagt
     6661 actcctgatg atttttaaga ttggtttgag aaataatgtg aaaacactat ttttaatgtt
     6721 aattatagat agagatgaac gcgtcatatg gttaaaagaa tgaaaaagtc aaagatacac
     6781 tgctatattt cactaaggat atagcagtgt atctttcatg tcgtttcact ggcagttttt
     6841 tataaaagga ggtgtgcatt tcagttgctt ttctttggta gttagttgaa tcgtttaaga
     6901 attaacggat gtggcagaca ggtgctctgt ccaaaaacca gtaaaatctt tgatggttcc
     6961 atctttattt agctgtgcct gttcaaatac tgttttgttt ttaccgaatg gatcaattgc
     7021 aataattgtt ccgatccccg cacgtttggc agcaagtaat cctgagtaag catcttcgat
     7081 aacaagacaa tcagccggag ctaagctcag tttgtctgcc gctttaagaa aaatatccgg
     7141 ttgcggtttc ccagggaaag agccgtcgtc atagacaatc ttgtcccagt caaaccagcg
     7201 ggctaaatcg aaaatatcaa aataaaaagc aacattctct ttgacagtag ccgtggcaat
     7261 cgttaatgga atctgttggt cagtcagctg gtcaagagtc gcttcaagcc cttttgtcaa
     7321 ctgaagctcg ttttcatttt gcaagcaaag catacgataa tagctttctt tttcttctga
     7381 caatacggcg atctcagagg cggtcaaatg ctctgaaatg aaatgtttca aaatctccgt
     7441 attcgtccga ccatgaatat tttttaaaat atcctcgtct gataaaccat cttttgtatg
     7501 atcatgtatc atatgcagcc aagcagcttc atgcagatga gagtccaaaa acattgttcc
     7561 gttgaaatca aatataatcc ctttcatagt tacgctccta tagtggtatt ttcggttgaa
     7621 tcagcaattt gcagatcgac atattgtgca tagtgctctt gaacctcccg cgagatctgg
     7681 cgatccgtaa tgagcagatc gaaatcatgc aaattataaa acacataaaa atcatctcga
     7741 ttgaattttt tattatcaac gagtagatat ttcttacggg aattatttag agcgatctgc
     7801 tgtgcttctc cttcttcggt actgtaagta gagatcgctt cgttgtgaac gccatttgaa
     7861 ctgataaatg cttttgtaaa ctttagtctt cttaaattgt cgttggtgat cggtccgaca
     7921 aatgcgccgg tatttttccg gaaatcaccg ccaatcagta gaatttctgg tccatctgct
     7981 tgagcaagaa tatcaaaaac tggataatta tttgtaatga tccgaatttt cttgttcagt
     8041 aaatgcgtcg ccaatagttc gattgttgta cctggtccta agaaaatcgt ttctccctcg
     8101 tgaatcaagg aggctgctgt ggcagcaatt tctttttttt cttgaatctg cagagtggct
     8161 ttttcagtgt gagaaagttc ttgatctaaa gtgtaggaaa gactttgagc ccctccatgg
     8221 acacgcagca gcttgcccga tttttctaat tcatctaaat ctctgcgaac ggtcatatca
     8281 gaaacatcta attgattgat gatttcatta acggtaagga ttcctttttt attgaccatt
     8341 tctacgatag taagcaatct ttcttttttc aacattgtct tcacctctaa tttaaggata
     8401 atctatcata taaacggaca acagtcaatc taattcgaac aaaagtaaac atttagggtg
     8461 gtttttatgt ttgttacaat agattttatt gctgttttct ggtttttatg attatttgtg
     8521 tttgtttttg ttgacaaatg ttttggatgc gtttacaatg caatcagtta gaaagagcga
     8581 aaactgatta tagatttgag gagagatgta catgcttaaa ttaccagaag attttatttt
     8641 tggcggagcc acagcagcct atcaggttga aggagccact aaggaaggtg gcaaaggcgc
     8701 tgttgcttgg gatgactttt tagaggaaca agggcgattc agtcccgatc cggcaagtga
     8761 tttttatcat caatatgcaa aggacattga attgtgtgaa cgttttggtg taaatggtct
     8821 aagattatcc attgcttgga gccgcatttt tcctgatgga gctgggaaac caaatccaga
     8881 aggaatcgct ttttatcatc gtgtgttcga agaatgtaag aaaagaaacg taacgccgtt
     8941 tgtgacgttg catcattttg atacccctaa acgtttgttt gaccatggag attttttgaa
     9001 tcgagaaaca atagaagcat ttgtttccta tgccattttt tgttttcatg aattcaaaga
     9061 agtcaaggtg tggagtacct ttaatgaaat ctatccagtt gcaaccaatc aatatttgtt
     9121 aggtgttttc ccaccaggaa tcaaatatga ttttacaaag atcgtcgcct gtcttcataa
     9181 tatgatggtt gctcatgccc gtgtagtgaa ctatttcaaa gaaaacgaac ttccgggtga
     9241 aattggtgta gttcactcat tagagacgaa atatgcggca acagacgcgc cggaagataa
     9301 gcatgctgcc tttttagacg atgcattgtc gatccgtttc ttattagatg ctacctattt
     9361 aggctattat tcaactgaaa ccttgactgc tcttgatgag atatgtgaag caaatcaggc
     9421 cagctatcat tttcctgaag aagatttcgt tgaattgaaa aaagcatcca cgcgtaatga
     9481 ctacttaggg atcaatcact accaatgcca ttttgtcaaa gcttacgatg gagaaaatgc
     9541 aatccatcat aacggtacgg gtgaaaaagg gacctctgtt tataaagtga agggaatcgg
     9601 agaacgtatt tataaagaag gaattcctcg aactgattgg gactggctga tttatccaga
     9661 aggtctatat gacttgttgc tgcgaattaa aagcgattac ccacattaca acaaaatcta
     9721 tatcactgag aacgggatgg ggtataaaga tcagtttgaa gatggcatta ttatggatca
     9781 gccgagaatc gactatttga gagtctattt ggaatcctta agcaaggcga taacagccgg
     9841 ggtaaatgtg aaggggtatt ttttgtggtc gcttatggat cttttctcat ggacaaatgg
     9901 gtataacaaa aggtatggct tgttttatgt tgattttgaa acccagaagc gttacccaaa
     9961 agaatcagct tactggtaca agttggtcag tgaaaccaaa acgattattt aataagtgat
    10021 aatggaggaa gaaaagatga ataaagagga attacaaatg cttggttttg agatcgttgc
    10081 atattcaggc gacgctcgca gtacattatt gaaattattg aaagaagtaa gacaaggaaa
    10141 ctttgaacat gttgattcgg cgttgaaaga agccgacgaa aatttaaccc ttgcacataa
    10201 ttcgcaaaca aaaattttag cggaagaagc atctgggaaa gagatggaga tgggattcat
    10261 ttttatccat gggcaggatc atttaatgac cactttattg ctgagagatc tgattcaaga
    10321 ctttattgta ctgtacagac agaatcaagg ataggagaag aacgctatgg atggaatcat
    10381 taaacaaatc gaaaaaggga aaccattttt tgaaaaaatc tcacgtaata gttatttagg
    10441 agccgttcgt gacggatttt taacagcgat gccagctatt ttattttcaa gcattttcat
    10501 tttgattgct gcggttcctg agatttttgg ttaccaatgg cctgaatctg tctccacatg
    10561 gctatggaaa gtctataatt attcgatggg tgttgtaggg ttactcgttt cagcaaccac
    10621 tgctcgctgc ttggctgatt cgatgaatcg tcgcttaccg aaaaacaaag taatcaacac
    10681 cacctctgtg atgctggcct ctgtggtaag tttcctgttg ttaagcgtct ctccgattga
    10741 aggtggattt tcaacggaat atatggggac aaagggacta ctggcttcct ttgtcgctgc
    10801 atttttaaca gttaatttat ataagttttg tgtgctgaaa gatattacca ttaaaatgcc
    10861 taaagaagtt cctggaacga tttcgcaaac cttccgagat atttttcctt tctctttttc
    10921 agtatttgcg gccgttttaa ttgatttagg cattcgacaa atcttccaaa tctcatttgc
    10981 agaagcaatc attacggtgc tgcagccgct atttacagca gctgacgggt acttaggaat
    11041 cgctattatc tggggagcga tggcgctgtt ttggtttgtc ggtgttcatg gaccgtcgat
    11101 cgtggagccg gcaattgcgg caattatcta tgccaatgtg gaaaccaatc tgcaattgtt
    11161 caattccggt gaacaagctt ctaaagtact gacggtgggt ttagggaatt ttgtaggaac
    11221 tatgggagga actggggcaa cattggttgt tccttttatc ttcctactct ttgctaagtc
    11281 taagcagttg aaggcagttg ggaaagcttc ctttattcca gtaagtttcg ctgtcaacga
    11341 accattgcta tttgcggcac ccatgatttt gaacccttat ttttttattc catttttgtt
    11401 aacacctgtt gtaaacgttt gcctgttcaa gttttttgtc gatgtattgg gcatgaacag
    11461 ctttatgtac gttttgcctt gggcaactcc agcaccgatc ggtttggttc taggaacggg
    11521 catgggtatc ttagcttttg ttctagcagc ggttctaatc gtcgttgact tcctgatcta
    11581 tctaccattt tgcaaagcat acgatgctgt cttagtagag aaagaagcac aaaaagcaga
    11641 ggtgatcgca accgaggaag gtgcttctgc cgatgtacgt gaggaagtac ctgtggcagc
    11701 gagtcaagtt atcagcgatg caaataaaga aatcaatgtg ttggtacttt gtgctggcgc
    11761 agggacaagt gcgatgttag ctaatgcttt aaaaaaaggt gcatctgaat cgaaagcacc
    11821 gatcagtgca atggcgggtg cttatggttc tcattacgaa atgctgcagg attttgatat
    11881 ggtggtctta gcaccgcaag tcaattctta ctatgatgat atcaaagaag acaccgatcg
    11941 cttaggaatc aagctggttg cgacaaaagg agcagaatat atcggattaa cgagagatcc
    12001 tgaaaaagct gttcagttta ttctgaacca atttcaataa aggaggagcc ataatgaaaa
    12061 tgggaatttg gttgcgtaaa gctgttttat ttgcagctgt gttaggggct ttgggggctg
    12121 tttcaaaaac tgttgaagca tctgccagcg aatttattcg cggagcagat atttcgatcc
    12181 tgtcagatat ggaaaaatct ggtgcaaaat attatgaaga cgatatttca aaagatgcct
    12241 tagagatttt aaaaaataat ggtgtcaact atgttcgact gcgtttgtgg caggatcctt
    12301 ataatagtaa tggagaaagt tatggagcag ggacaaatga tctggaaacg acgattgacc
    12361 ttgcaaaacg agcaaaaaat ctgggactta aggtgctgct ggattttcac tacagtgatt
    12421 tttgggttga tcctgggaaa cagaatcttc ctaaagcatg gcaaggtctg acctttgaag
    12481 aaatgaatac ggcattgtac gactatacaa agaacgttct gtcggaaatg aagcagctgg
    12541 acgtttatcc tgatatggtt cagatcggca atgaactgaa tagcggaatg ctctggcctt
    12601 atggcaagtc ttggggagaa ggcgggggag aatttgatcg attggctgct tttttgaagt
    12661 ctggtatcca agctgttcga gacactcaac ctaaaacaac tccagtaatg ctccacttag
    12721 ctgatggtgg cgatacagga gcatttactt ggtggtttga tgaaatcacg agtcgaggtg
    12781 tgtcatttga tttaattgga gtgtcgtatt atccgtattg gcacggttca atgaatgaac
    12841 ttcaaaccaa tatgaatgcg atcagccaac ggtatggtaa agaagtgatc gtagttgaaa
    12901 cagcttatgg gcatacaaca gcgaatgccg atactatgcc aaatgctttt ggtgaagccg
    12961 aagcagctgc ggggggatat gaaccaacac cgacaggaca agcagagtat ttgctggatc
    13021 ttgccgatcg gattcaggca gtaccgaata atcgcggggc gggcttcttc tattgggaac
    13081 ctctttggta caacggaaat gtgagttggg caacacaagc aggtatgaat tatctcagtg
    13141 ttcaatccac tatgggaaac gaatgggaca accaagcgat gtttgatttt gctggaaacg
    13201 ccttgccaag cttgcgtgcg tttaaacaag caggggcgca aataaatcta gttaaaaatg
    13261 caagtttcga aagcgatgga ccaacgacca gtccttccaa ttggcaaacc tggtttcaac
    13321 aaggaaccgc taagggtacg atcaaaacgg aagcgggcac tttttttggg gactacaaac
    13381 taactttttg gcaggatcaa gcatacgagg catcagctta tcaaaatgtt agtggcataa
    13441 gcaaaggaac ttacacattg agtgcctatg taatgtctgg gggagagttt gatactaatc
    13501 agttatatat caaaaattat ggtggagcgg aactaaatac tgcggttgtt aaaacaagtg
    13561 aatggacaaa aattacaatc aaagacattc caattacaac aggtgtttgt gaaattgggg
    13621 tttatagcaa ggcttcagga aatgcttgga cgaacgttga ttttgtttca ttggtgaaac
    13681 aatagactgc ggatcgtttc gattcaagat cggaggttcc cttcagccac atcgttagcg
    13741 tagaaggcag gaatcgaacc tgcacctcgc ctttgccaaa aataaccaga aacgatttcc
    13801 agtcattttt accaaaacga gtaaagcacc agacttcatc tacactttgt attatgctaa
    13861 agagagttca acgatttcgt aagaagcaat gaaaaaattg caaaaagttg tgcagataac
    13921 ataaagtttg tttaagaaag ggtaacctta gaattccgtc gtgaatatga tttgagcact
    13981 tagatggata gaaaaaagtt ctcggaaaac agttcgtttt tcgagaactt ctttttatga
    14041 taagaatgaa acaaattaac ttatattata gtctcttttg gtagggaacc atcttatttt
    14101 taatgaaaat caagcatttt agctattgaa gaatgttgat ttatagggat tccccctcca
    14161 tattatgttg gctctttatt cccactctgt tttcaacgta aagtgtataa aacagaatgc
    14221 gatactatca agggagttaa aacaatatac agaacagtaa aacactataa ataaaaataa
    14281 aagtgcaata aaagtgcaat ttttgcacct aatcatactc tgtttatata tgaaaactag
    14341 attctgaatc caatatttgg ttaacgagag gcgttcgaat ttgaacgtct tttatatatt
    14401 actttcaaat aaatatgagg tagaatacta tctattttct ttttaaccca attgttataa
    14461 t
//
LOCUS       DARBEC010000070        14390 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.072, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000070 DARBEC010000000
VERSION     DARBEC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.072"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(36..932)
                     /locus_tag="SCL80_002046"
     CDS             complement(36..932)
                     /locus_tag="SCL80_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1107900.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(925..1605)
                     /locus_tag="SCL80_002047"
     CDS             complement(925..1605)
                     /locus_tag="SCL80_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1107901.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(1606..2388)
                     /locus_tag="SCL80_002048"
     CDS             complement(1606..2388)
                     /locus_tag="SCL80_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1107902.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(2381..3298)
                     /locus_tag="SCL80_002049"
     CDS             complement(2381..3298)
                     /locus_tag="SCL80_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1107903.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(3462..3785)
                     /locus_tag="SCL80_002050"
     CDS             complement(3462..3785)
                     /locus_tag="SCL80_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107904.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            4573..7800
                     /gene="fss3"
                     /locus_tag="SCL80_002051"
     CDS             4573..7800
                     /gene="fss3"
                     /locus_tag="SCL80_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEG1107905.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYWQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            7913..8227
                     /locus_tag="SCL80_002052"
     CDS             7913..8227
                     /locus_tag="SCL80_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1107906.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            8240..8614
                     /locus_tag="SCL80_002053"
     CDS             8240..8614
                     /locus_tag="SCL80_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1107907.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            8624..8959
                     /locus_tag="SCL80_002054"
     CDS             8624..8959
                     /locus_tag="SCL80_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEG1107908.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            9042..10382
                     /locus_tag="SCL80_002055"
     CDS             9042..10382
                     /locus_tag="SCL80_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1107909.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            10460..11134
                     /locus_tag="SCL80_002056"
     CDS             10460..11134
                     /locus_tag="SCL80_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEG1107910.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            11127..11291
                     /locus_tag="SCL80_002057"
     CDS             11127..11291
                     /locus_tag="SCL80_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107911.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            11367..12509
                     /gene="dcm"
                     /locus_tag="SCL80_002058"
                     /pseudo
     CDS             11367..12509
                     /gene="dcm"
                     /locus_tag="SCL80_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            12499..12789
                     /locus_tag="SCL80_002059"
     CDS             12499..12789
                     /locus_tag="SCL80_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107912.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            13048..14232
                     /locus_tag="SCL80_002060"
     CDS             13048..14232
                     /locus_tag="SCL80_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1107913.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            14229..14366
                     /locus_tag="SCL80_002061"
     CDS             14229..14366
                     /locus_tag="SCL80_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1107914.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
BASE COUNT         4598 a         2634 c         3019 g         4139 t
ORIGIN      
        1 acatttttct actagttctc actaatcttg tataattaaa ccctccgcaa cttgacggag
       61 atggagaaga tgcccccgtc taatctagcg gtcatctctc ctcccattcc ttccaccaat
      121 ttttttgaca ggtaaagccc gattccgcta gattccgtat ttgtgcggct ggtattttcg
      181 gtataaaaac gctggaagac tttttcaaca gaggtttgcg ttcctggttt gataggattg
      241 gccactgtta atcgaatgaa tggatcctct tttgaaaatg tcacctttgc ttgtccatct
      301 gcataacgca ataaattctg tatgatattt tgcagcactc gcagcaacat gagcggatct
      361 gctaatacag ttgtatttat cgagtgttca gcgaacgcta cctcgatatt tttctcagaa
      421 aattgttcat aaaaagagaa aaatacttct tccacctctt tagaaaaaga aagcgtatgg
      481 agatccattt ctttttgctt cgtctctatt cgagcaagat cataaaactg atccgtcata
      541 ctgagtaatc ttgcaacaga ttgtttgatg atcgttaaat acttctcccg tttctccgta
      601 tcctctacct gttccagtaa ttgtacataa ccgttaatag cagtcaaagg agttctgaga
      661 tcatgagaaa ggccgacgat cgatagtctg atgttttctt ccatttcttg aaatgcttgt
      721 cctttttgtt cattttcatc caaccatgta ttcagtgcat ctactaggtc cattagattc
      781 ttttctcgaa attcgactga aagacgacca ccatattctt gtcgttcaga aagagtggcg
      841 attttcttag ttaatctctt gatttccctt tgcaggaaga aaataaaact tcccataaag
      901 ataacaacga gaagcaacag ccatttaatc aaatctaaac cccactcccc acaccgtatg
      961 aatatacaca gcatgtgcct ttgctagctt ccctcgaaga ttggaaatat gcatattcac
     1021 tgttttctca ctatcaaaat atggttcttg ccaaactgtt tcataaagat ttgctcgcga
     1081 aaatactttt ttcggatgtt ctaaaaaaag cttcagcaga tcgaattctc ttcctgtcaa
     1141 atggacagcc acaccattca catttacatc cctcgactct ttgtccagta agatttcctt
     1201 gtaatagatg cgttgcgaca gatcttttac ttggacagaa gtctgatgac gaagttggat
     1261 gccgattcgt aacaaaagtt cttcgatatc aaatggtttc gtcacatagt catccgctcc
     1321 tgctgccaac aaggaaactt tatctctttg atcgcttttt gctgaaagaa cgatgatcgg
     1381 aacattcgat gtctgtcgga tttctgctaa caatgcttca cctgaaagcc ctggaagcat
     1441 gagatctaga aggatcaaat cgacaggata tgtgcttaac aataattttc cttccgtacc
     1501 tgaataagca tcaagaagtt tgtatctctg ttcaagaacc tcctccagta aggaatgaac
     1561 agcttcatcg tcttcgataa tcaaaatagt ttccagcagg accacctact taatgtcaga
     1621 tttttggaaa ataaacattc ctagagctga tgataccacg attgttacta gactgaccag
     1681 aatcattttc ggtgcttgcg aataagttgc ttgttgcgca aagtccgtat acatgagcca
     1741 cgtttccgta aaccactccg ttagaaattc caagcctttg atttttgcga aaacaccggg
     1801 aatgaaggcc gttcccatga tataaagaaa agaaagaagc aaactgacac tggtacttct
     1861 agtgatgaat gtcaaagcca ctggaacaga taatgtccct aatgtcagca gaagcaatac
     1921 aggaaatact tttagaacat aatttccata gtttttgatt ccctcaactt caccaaatcc
     1981 gaaagcaagt gtgtaagcaa gcatggctag tcccgtgatc aaaaccacgc caagtgttgt
     2041 gatcaaaagc gtaacgagaa gtttcgacac ataataagtc attcgattcc gtcctaaaga
     2101 taacgtattg cgaatcgtac cgttttgaaa ttcttcccca atataaaagc acgcaaatgc
     2161 tgcgaaagcc agaagaatcc atgcagccat tttttctacc attaaaatcc ctgttgcacc
     2221 gttgatgact gtatccgttg cttggtttaa ttcattgccg tattttgtat gcatatacca
     2281 ttcgttccaa gaataaaaaa gcggaagtac gaccatcagt cctagtgaga cccacatttt
     2341 acggctcttc catactttta ttttttcagc agttaataga ttaaacatgt ggattgcctc
     2401 cagttaattg taagaaataa tcttccagtt tttgcccaac taggtgaata ccatccacag
     2461 gaacttttgc ttctaccaat gcttgattga tggctgctac ttggtcaatt ccttcaaaaa
     2521 gctggataac acggctagat tgtacaaaat aatccctata accaagagag tcgagaaccg
     2581 ttactgcttg ttccgtcgcc gaagtgtcaa tttttataaa ttgacgagat tcttgtgcca
     2641 attcttcttt cgataattct ttgatcaagt gtccttcatg caaaatacca tagtgagtgg
     2701 cgatctgtga caattcatct agcaagtggc tagaaagcaa gacggtgatg ccccgttctt
     2761 ttaccaaacg ttggatgatt tctcgcattt ctgccatccc tgctggatcc atcccattcg
     2821 tcggttcatc caaaatcaaa aattctggat tcgtgatcag tgcatttgca atagctaaac
     2881 gctggcgcat acctaaagag aaattctttg ctttcttttt ccctgtattt tccaatcgca
     2941 tcattttcag caggtcttcg atttctctat ccgacacacc gccgttcgct gcttggatac
     3001 gtaggttatc tcttgcagtt aattctggat aaagagcagg ggtttcaatc gattgaccca
     3061 tacgtcgtct tgctgcctgc aaatccgtcg ttccaaataa ttcgatcgaa ccttcatcaa
     3121 tcgagatcaa acccataata gttctcataa aggtagactt acccgctcca ttttccccaa
     3181 tcaatccata gatcattcca cgtttgattt cgattgatac tttatctaat gctttggcag
     3241 caccgtattt tttggttata ccagttgctt tcaagaccgt ttcatttcca aaatccatac
     3301 ttatccttct ttcttattga tgagtgaagt atagttgacg ggtataaaga aagcagccag
     3361 aaatgtataa agaaagtgta aattttccaa ttgaatgtca aaaaaaacaa tcggacaata
     3421 cgtgtctttc ctattatcag gcagtttgtt atacgcttgt atcagtttcc tgattctttc
     3481 ttttcgacca aagtcgtttc tctgtattcc tcttcactga aaatttttgc ttctttcaga
     3541 tcaagcttga gtaattttac cagaccggtt ataaggataa ctatagcaag actccctccc
     3601 agaaaaatta gactgtctcg cagacgtggt tgcgttgtct tccaatcaaa gagcgcacca
     3661 aacagtgcta atccaagtgg actggccacc atggtaatcg ttgttatcgt tgacataagt
     3721 cgtccttgta tatgcgttgg aacttctcgc tgaagccaga ccatataaaa agcaaaagaa
     3781 aacattaatc cttaaaaacg gacttatgaa aaataataca tgccgtttgg gatttacaat
     3841 tagctaccaa ataagaggtt atgacaaaag acttttcaca atcccttctt ccgaataaac
     3901 ggtgttctcc tgccaactcc tcggtcacaa gtcacaatat ttatcaaaca tgagaagcaa
     3961 tttttgtaaa ttccttctta tttcttgaag ctgaccactt ctgtcacaac ctcagaagcg
     4021 gataaataat attgtttgat gatactttat ctttttcccg tttgtttata gatcgaatga
     4081 tattatagac caagaatcct acctattaac atagctaaaa acacaaaaaa ggcactttcc
     4141 aaattgtgga aagtgccaga aaagttagga ttaaggtaac tgcgctcagc attgcttcgc
     4201 ccagtacgat tctcgtaact cgtgaactcg aacgataatc gcaattaacg ttttgagaac
     4261 tgtgaagctt tacgggcttt cttaagacct ggttttttct tcgtatcatt gaataagttt
     4321 gaaaaacctt gatttataaa ggttttcccg taaatatatt ttattatatt tgattaaatt
     4381 tgaaatgttt gtgttctaaa atgtgttcta atttaaaggc agtatatttc gtattttaga
     4441 ttaaattttt aggctcattc attctcaaat ccattctatt cagtcaataa aaattttaaa
     4501 aagactttat aaacaaagat gttcaagcga gcatcttatt tttttgtgct taatttgaaa
     4561 ggagaacatt tcatgtataa aaaacaaaaa aagaaattat gtgtattaac tattttgagt
     4621 ttggctgttc aagcaattgt tggagcaatt gctcctacga ttacttttgc tgatgaaatt
     4681 acccatccac aaacggtaac cgtagaacta gatttagcac atcaatatgc tgttgaagga
     4741 acttttagtg atggtcgtcc aatgtcagaa gtcactgttc cgcattatgc agtgtacaac
     4801 ggctttaaac aagatatttt ctgtattgaa ccaggagtcc cgatttacaa tgagttcact
     4861 cctggctatg agaaaaaccc gttaccagat atgtccgata aagccaaatt agtttccgtt
     4921 ttatggaaaa aagtaggtac agatgcagat actcatattg tggcacagaa gatgatctgg
     4981 caagaagtta atgggtatac ccttcactca ataaaacgat cagatggtag tgccgtaaat
     5041 atcgcagcca ttgaggcaaa aataaatcaa gccattgacg attatcagaa gaaaccaacc
     5101 ttccataata gtacggctaa aacagtatta gggcaatcga ctactgtgac ggatacgaat
     5161 aatctgaatt tgtcagagtt cgatgaggtt gtggaaaatg ccgcaaacat tgattatcgt
     5221 gtgaatggta atcagttgat aatcacccca aatgccaatt caaaagaaag tggtgtgctg
     5281 acgcttaaga aatctgctgg taccggaaca cctgtcgcct ataaaaaggc tggccaacag
     5341 actttaatgg ccggggcgat tgataaacct aatacctaca caatcaaaat tgatgtggaa
     5401 acggagggtt ctttaaagat caaaaaagtc gataaagaat cgggtgatgt tgtaccagga
     5461 acggttttcc atttagactt tggaaaaact ttacctgcaa aagacgtgac tactgacaaa
     5521 gaaggcattg ctacattgga tgagattcct catggtacaa aagtaaccat taccgaaaaa
     5581 tcagtaccgg cgccttatac gattgatacc acccccatga ctaccaccat taaagcaggc
     5641 gaaaccattt acgtgacttc aaaaaacgca cgggaaaaag ggcaaatcat tctggataaa
     5701 agcggagttg aaacagggag tgatctttgg aatgacaatt attccttagc tggtaatacg
     5761 tttgccattc gcaaagacag tccaaccggt gaaatcgttc aagaaatgac cacggatgaa
     5821 aacggtcatg cggaaacacc aaaagagatt gctaatgcgt tggaattggg aacctattat
     5881 gtgaccgaaa ctaaagctag tcatgggttc gtgaatacgt tcaaacctgt aaaagtcgaa
     5941 ttgaagtatg ccaatcaaac cgtagcccct gttaccagta acgtaaaagg gcaaaatcaa
     6001 gaagtcactg gcgaaaccac tttgacgaaa gaagacaaag atactgggga taaagcacaa
     6061 ggtaaggcag tctttgaggg tgctgaatac accttgttta ctgctaagga tggaaaagct
     6121 gttaaatgga gtgaggcttt taaaccagag ttagcgaagg gaacgaaagg ttctgatgaa
     6181 acggtcactt tgactttaga cgaaaagaac caagttgccg ttaaacattt agctatcaac
     6241 gagtattatt ggcaagaaac aaaagcgccc gaaggatata gccttgatga aacaaagtat
     6301 cctgtatcca tcaaaaaggt ggatgatagc gaaaaaaatg ccgtgattac ccgaaatgtt
     6361 acggcaaaag aacaagtgat tcgctttggc tttgatttct ttaaatttgc tggatcagct
     6421 gcgggcacta ccgaaactgg cttcaatgat ctgaccttta aagtgtcgcc attggaaggg
     6481 accaatgaaa tcacaggtgc cgaagatgaa gcgaccacag cttataacga gcaattaggg
     6541 tttgatggtt atggcaagtt tgaaaatctt ccttatgggg attatttact tgaagaagta
     6601 gaagccccag aaggttttca aaagattacg ccattagaaa tccgttctac ttttaaggaa
     6661 aacaaagaag actttgtgaa gagtgagtat gtctttacca ttaccgaaca agaccaaaaa
     6721 caaccaatta aaacggtgac cgttccttac gagaagctga cgaacaaagc attttctgtt
     6781 agtttgaacc gtttaatgct ctatgatttg cctgaggaag aagatagttt aacttctctt
     6841 gcgacttgga aagacggaaa caaagaatta acttctctgg attctaccga gctagttgat
     6901 aaattgagtt acaatctaca tgaaatcaaa gaagactggt atgtggtagc gcaagccatt
     6961 gatgtggatg cgacaaaagc tgcccaagaa aaagatgaaa aagccaaacc agtggtgatt
     7021 gcagaaacaa gcgcaacgtt ggcgaacaaa gagaaaacag gaacttggaa aattcagcat
     7081 aaattaaccg ccgaacaagt tttaaataaa actattgtct tattcaacta tgtgtatgaa
     7141 aataaggaag cttttgaagc aggcgataag ccagtagcga aggatgttag cttgaataac
     7201 caagcccaaa ctgtcagttg tacagtggaa caccatgttt ctatccaaac aaaggcccac
     7261 ctagaaaacg gttctcagac ctttactcat ggtgatgtgg tggatatgtt tgatgatgtg
     7321 tcgattactc atgatgtact ggatggctca aaagaagctt tcgaaacaat tctgtatgct
     7381 ttattaccgg atggtacaaa caaagaaatt tggaaatctg gcaagattga ttatgaagta
     7441 aatgataaag aattcaccaa aactgtgctt gcgaaaaaag tagataccgg aaaatatcca
     7501 gaaggaacca agtttacttt tgcggaaatc aattacgata aagatgggaa tatcaatgga
     7561 aaacacaatg aagatttgaa agaaaaatct caaaccttaa caccaaaaga agttccaacc
     7621 atactaagta cgccgaaaca accggaaaca ccaactgttc caagtggctc tcaagaatct
     7681 agtcccacag tgaagacctt ccctcaaacc ggggagaaaa attccaatgt tttactgttc
     7741 attggtttta ctttgatttt tgcgacggcg ggctattatt tctggaatcg ccggaactaa
     7801 ggtgatgaga tgatacaagt aaatcctaag aaccaacgtg cgcgaccgcc tcctaaaaaa
     7861 gtagtcgcgc tatttttgta tccaaaacta acaatgaaaa gaggaaaaga agatggaatt
     7921 gaaatttgtc gtgccagata tggctgaaac ctttggaaag ttaagttatg ccggtgaagg
     7981 agaaattata acagaaggat atggacggaa tactacggtg attggtcgca gttatcattt
     8041 gtattccagt aagcaacggg ccgatgatat tgaggtggta gtagctgcgg aagccggtga
     8101 aaaggatttt gatcaagacc aaccgctaaa agccgtgaat ccccatttgg ttgccaaagg
     8161 ctatgagatt gagaatcgtg ggtttactga ttacgtgttg tatgttgatg atttagtgaa
     8221 agcataggag gaaaagaaga tgagattaac agaaggtatt gtcgtagatt caggtttaac
     8281 atttgggaag ttgcgttttt ccgcattacg ccgagaagta cggaagcaaa atgaggatgg
     8341 aacaattggt aatgaggtaa aagaacgaac ctataattta aaatcctctg cacaagggcg
     8401 aatgattcaa gttagtatcc cagctaatgt acccttacgc gagtttgctt atgatgcaga
     8461 ggtagagttg gtcaatccaa tcgtggatac cgttgccaat tatgtttttc gagaaggaac
     8521 taccgttaat tggttcatta aggcggatga tttagtcttg aagcggcagc cgaatcaagg
     8581 aaatcctacg aaccaaaatg aaagaaagaa ataggtgaag attatggaaa ttaatgttga
     8641 acatatttta gattgtctcg atcaatatgg aaaaggcgag ctgactgagg aacaactcgt
     8701 taaagcatta acctctgatg aaaaaatgtt tctgattata catcaaggtt tacttggaga
     8761 aaatagcgac agtgaagaag actctgatgt actagactgg ttaggagaag aagagtcttt
     8821 ctttatagtt gtcgaagtaa atgaaaactt atgtcggcag gcagaatcca ttttggaaga
     8881 aattggtgtg gagatgccgg atgctattga aggcttttta aatcaattgg ttgaaacgaa
     8941 acaactcccc tcctcgtagc agtaaatgac tagcataact ttttgaaatt gcctaatgaa
     9001 atcctattgg gctttttcga ttggaggtgg aaaccatcaa aatgtacaaa ggacatcgca
     9061 tacgcgcagg agatcagcac ttggtttatc actttgttct tggctggcta ctcgctctgt
     9121 tcatcggttg gatgagcgtc ttttattttc aagagtttag acaatttgat atttctaagc
     9181 tatcactttc tactattgaa atcgtttggt ctataaaaga tttagtatgt ctgcttggaa
     9241 gtctcgcttt ttcaggagct atgatattgc tttatattca cttttttctt gatcattggc
     9301 gaagcttgtg gcatcgacaa aagctagctc ggatgattct agagaaccat tggtatgaag
     9361 tgaaacaaac tcaaagtgaa ggctttttca aagatttgaa tagtagccga accagagaga
     9421 ctatcagtta cttccccaaa atctgttatc gaatgaaaga tggattactg tctattcgcg
     9481 ttcaaatttc acttgggaaa taccaagatc agcttttaaa attggaaaag aagttagaaa
     9541 gcggactgta ttgtgagtta gtagaaaagg aactcaaaga ctcctatgtg gagtatactt
     9601 tactgtatga tatgatcgcc aatcgaattg ggatagacga agtagtggca gaaaatggga
     9661 ctctgcgatt gatgaaaaat caagtatggg cttatgattc tttaccccat atgttaatcg
     9721 ctggtggcac gggtggaggg aagacctatt tcctcctcac cattatcgaa gcactattga
     9781 agtcagatgc agaattgttt atcctcgatc ccaaaaatgc agatttagct gatttaggta
     9841 cggtgatgcc tcatgtttat tcacaaaagg aagaaatttc tgcttgtgtg gaagatttct
     9901 atgaacgtat gatggctcgt agcaaggcaa tgaaagaaat gtctaactac aaaacaggag
     9961 agaattatgc ctatcttgga cttccaccca actttttaat ctttgatgaa tacgtggctt
    10021 atatggagat gttaacgacg aaagaaagtg cggtgatttt gaataagctc aaacaaatcg
    10081 tgatgttggg tcgtcaatct ggtttctttc tgattttggc ttgccaaagg ccggatgcga
    10141 aatatttagg agacggaatc cgcgatcaat tcaactttcg cgtggctttg ggtcgtatga
    10201 gtgaactcgg atattccatg atgtttgggg aggtcgaaaa aaacttcttt atgaagcaca
    10261 tcaaaggtcg aggttatgtg gatacaggtg gcagtgtgat tagtgagttt tatacgccac
    10321 ttgtaccaaa agggtatgat ttcttagatt caattagtaa attagaaaag atgaatgatt
    10381 gagtgatctt caatactgac tttatacttc aagtaacgta taatactttt atatagtagg
    10441 aagaaagggg ataattaata tggaaagtaa tgattcttat ttagagtatg caaaagcaaa
    10501 taaggataag tttatttctt atattatcaa agggaaatcc cccgaagttg gacgagacgc
    10561 agtttttatg gcagggagtc ctggggctgg aaaatcagag gttgccattg gattagctga
    10621 gaattatgat aaccatgtga ttattgatgc ggattacttt cgcacccagt ttcctgatta
    10681 taatggaaaa aactctagtc tctttcaaaa agcatcttct tggttggtag agcaggcatt
    10741 tagatatgtc ttagatcatg gctattcttt tattctggat gcaacttttg caatattaag
    10801 tgctgaaaaa aatatctcgc gggcgttgaa aaaacatttt cgagtgacga ttttctatgt
    10861 ttatcaagac cctaaaatcg cttggaaatt tacacaagaa cgtgaaattg cagagggacg
    10921 tcatgtgccg aaaaaggcat ttattaacgc ttttttcaaa tcaagagaga acattgcaaa
    10981 agtaaagagt aaacatccag atgtattgct tcatatcata ataaaagatt atacgaataa
    11041 tatttccgaa gttcattatg ctacggataa tctagaatta tttttaccac tagggtatag
    11101 taggaaagat ttggaggttc aattacatga ctgagaaaga acaagtaacg aaaattgtaa
    11161 agaaatacaa taagagcatt gcggatttat cggaaaatgc tactgcaaaa gagttcaaaa
    11221 cggtgattaa atatgtagcc gatcaggcca atgaaaaaca acggaaatta gttggattaa
    11281 ataaaaaata gtaggacaaa ttaataatgt atgagccaag agcaaaatgt tcttggcttt
    11341 tttctttgca atgaatgagg taatgtatga gatttttaga tttattcgcc ggtattggtg
    11401 gctttcgttt aggtatggaa caggcgagcc atcattgtat cggtttttgt gaaattgata
    11461 aatttgctag acgtagttat aaagcaattc atgatacaag taaggaggtg gaaatgcatg
    11521 acatcacaag tgtatcagat gagtttattc aatccctcgg accagtggac atcctttgcg
    11581 gcggatttcc gtgccaagct ttttcaattg cgggaaagcg gcaaagattt tccgatactc
    11641 gaggtacttt attctttgaa atcgctcggt tcaccactct tctccgacct aagcttttat
    11701 tccttgagaa cgtccgagga ttgctcaatc acgaaggagg ggctacgttc gagacgatcc
    11761 tccgaacgct ggatggattg gggtatgacg tggagtggta ggtgcttaac tcaaaggcct
    11821 acattcccca aagccgggag cggatattcc ttatcggaca ttctcgagac gcatgtaccg
    11881 aacaagtatt tcctatcatt ggaccgtctt caacatctga tcaaaacatc agaaatttgg
    11941 taaatatcaa tccttccaat cgaggaatgg gtggacaagt ctatggttca gatggcgtag
    12001 cgccaacctt aacaggtgat gaaggaatca aaatcgcact gccagcaagg gatggaatct
    12061 ctgtagcagg actattaccg ggaaagtttg aacaaggtaa tcgagtttat gaagttactg
    12121 gaaccgcccc caccttgtca acgaaacaag gtggtacaaa aatcatgatt cgaaaaaatg
    12181 aaacagaata tcaagaggtg aagccagaag acagtgtgaa tctggctttt cctaattcca
    12241 caagtcgccg aggtcgtctg ggaaaacaga gtgttcatac cttactaaca ggagaccaac
    12301 aagcgatcgt cacggatcag tatcaaattc ggaagctaac acctagagag tgttggcgac
    12361 ttcagggctt tcccgattgg gcttttgatc gagattctca agtcaattca gatagtcagt
    12421 tgtataagca agccggaaac tctgtaacag ttccggtcat ttttgatatt gccagaagat
    12481 taaaagagtt ggatgtacat ggtttatgat gagttgattg gagaaattta ttgggtgatt
    12541 ggaaagattc agtcagaccc agaattagaa gaacaattgc gacggttaaa atttgatatt
    12601 cgcaagaatg gtgtcaaagt cgctggcgat ccatatttaa tgaatgagga gacggatgct
    12661 cgaattgaaa taaatcaagt aattgcagaa tttgagcgga ttgcggacca agcaaaagaa
    12721 ccagatattc gtcaatactt gttagaaatg aaagcagagt tggaagtaaa tggtattaca
    12781 gatgagtaac ctaaaccaac acaactgact gttaggcgct gaccgcggag gattcgcgaa
    12841 gcagaaatcc tccgtggtgc tgtgcctgtt catggacggc gttagccgac catgatgtct
    12901 cacccccgta tctaacaggg gggtacaaaa acacaaaaaa taagtaagca accattaaga
    12961 atgcttgttg tatcaaagat agtaagtaat ttgtataggt gttaactcat ttgaaaaagt
    13021 taacaccttg aatttggagg tgagaacttg gcacaacgga atttagatta tcggctatta
    13081 aaagaccgac gcaacgaata cggcgtttca caaaataaat tggctacggc ttgtggactt
    13141 agtcggccgt acttaaatca gatagaaaat ggtggcgtaa ccgcatctat aaaaaccatg
    13201 aggaaaattt ttgaccaact ggaaagcttt aatcccgatt tgcctttgac gttactgttt
    13261 gactatgtaa ggattcgctt tcccacaacg gatgcacgaa aaattattca agaaattctc
    13321 catttgaaat ttgagtatat gctccatgaa gattatgcct tttactccta tcaagaacag
    13381 tatgtcatgg gtgatattgt ggtgatgttg tctcatgaag aagataaagg tgtgctttta
    13441 gaattaaagg ggcgaggttg ccgacaattt gaaacttttt tactcgctca aaagcgtagc
    13501 tggtacgact ttttcgagga ttgtctaaag gccggtggcg tgatgaaacg cttggacttg
    13561 gcaatcaatg atcgagtggg gctattggat atacctgagc taacgaaaaa atgtcagaag
    13621 gaagaatgta tctccttgtt tcgtaccttt aaaagttatc gttccgggga acttttaaag
    13681 gctgatgaaa aggacggcat gggcaatacc ttatatatcg gcagtctcaa gagtgaggtt
    13741 tatttctgtt tgtatgaaaa agactatgaa caatatatca agttagggat tccattagac
    13801 caaactgaga cgaaaaatcg ttttgagatt cgactgaaaa atgaccgtgc ttttcatgcg
    13861 attcaagatt tgttaaaagg tcgtagcatt gagagcacca cgttttccat tatcaatcgt
    13921 tacttgcgat ttgcggataa agtagaaggt aaaaggcgaa ccaactggcc cttgaatgaa
    13981 caatgggggc gttttattgg acggaatcgg aaagagattc aattaacttc cgagccaaaa
    14041 ccgtatacca ttgaacgaac cttgaattgg ctaggccgac aagtcgcccc tacgtggaaa
    14101 atggctaagg agttggatcg attgaatcgg acaacctata tccaggatat ggtacagaat
    14161 gcacgcttat cagaccgaca taaaaagatt ttggagcaac aaagtatggc aattgaaaat
    14221 ttgattatat gaaaagagga atcgtcatgg gaattgtaaa agatattttt ttggtatttg
    14281 gtggcagtat gcttggtgtt actgtgatgt gcttgatgaa tgcaagtgca gcagcggatc
    14341 gagcaatgga aaagaaagga aagtgacaag atgaatttcg gacaaaattt
//
LOCUS       DARBEC010000071        13810 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.073, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000071 DARBEC010000000
VERSION     DARBEC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.073"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(221..1423)
                     /locus_tag="SCL80_002062"
     CDS             complement(221..1423)
                     /locus_tag="SCL80_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1107915.1"
                     /translation="MKTKYPCVYQDKNGRFSYLVELGTDQVTGKRIQKKGTKDSLGKR
                     FSSAREAHKEVMRIKNEYMVVNGFANYDLTYKEFMEQSYVPHYKASVQKSTWSSRECG
                     LIQITEFFGDKKLREINVRDCENYRIWLLNDSGYSQAYSSLMYGMFRKSLDYAVTLQF
                     LNENISKRTKAIPKGKSIVPYWTKEEFEEVLAKIYKDDFYEHMCFLMIWLYYMTGVRV
                     SEGLALNWNDVDFKRKKLRVHHTLEMTNQKDFIRKPYTKTESGLRIISLDDDTIEVLK
                     KWKVIQKNHGVEQFILSYTDLPLYRSTVQRIIQRYAKLAKVPLIQGKGLRHSHVSYLI
                     NEFNADILVVSQRLGHSSPEITLKHYAHLWSRNDEGIAEQMTGNIKFTCATKSKVANF
                     NGNQAVKI"
     gene            complement(1426..1620)
                     /locus_tag="SCL80_002063"
     CDS             complement(1426..1620)
                     /locus_tag="SCL80_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 domain-containing protein"
                     /protein_id="HEG1107916.1"
                     /translation="MKNTICRKDLEALGFKTHQATTIIRQAKTRMVNQGYPYYNNKRV
                     GVVPRQVIEEIIGVPLSQEE"
     gene            complement(1667..2515)
                     /locus_tag="SCL80_002064"
     CDS             complement(1667..2515)
                     /locus_tag="SCL80_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rep protein"
                     /protein_id="HEG1107917.1"
                     /translation="MQAYNQKIIETPTYIEIYDYEAIITPKRKTIENEVNLERKQKLA
                     WIYENDITLEKRKNYDELSAQGQYDSLKRKQKHYQSMRFEIARLVDTNFDNQTKFLTL
                     TFKENIQDIEYANNEFKKFIKRLNYDLYKTKKANLKYLATWEKQQRGAIHYHIILFDF
                     PFISFNRLTTIWGQGFVKINKIDVDSIENRGRYISKYFDKELELKEHKKKAFFKSRNL
                     KLPTVTKKMVKKPYDSTNQDILYTSEYIVRRPFFYNALALNELGSPEQVMDFQESTVH
                     YTKIKK"
     gene            complement(2720..3070)
                     /locus_tag="SCL80_002065"
     CDS             complement(2720..3070)
                     /locus_tag="SCL80_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002401157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107918.1"
                     /translation="MPIKITDLSLPKETLEKEYQVVNVTRWQKEGEILGWSYECILPK
                     LRYEKISVKVKSEHPVITPEELTQQGLAIITFTNLVIVPWGRSNGQFVSYGLSATADT
                     ATLIPRKQGTTTQN"
     gene            3665..4111
                     /locus_tag="SCL80_002066"
     CDS             3665..4111
                     /locus_tag="SCL80_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEG1107919.1"
                     /translation="MKDYVVQITKEVLEESKTLNKLSSKAKEEAVTILSSGIIDYIEM
                     SGEIKYGEKDRIAKMVKESIKTFDVKQDLTFNDSYLIGKLINILGTDEDTELFLSSLS
                     MIFTDKHFSTRFGGEELVEIFQSMRPALIKLYREKTPDEFYDWFEK"
     gene            4735..5727
                     /locus_tag="SCL80_002067"
     CDS             4735..5727
                     /locus_tag="SCL80_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1107920.1"
                     /translation="MYWEEIELEKTLGPGGEIRAVQRDKKNISKWEKLKKQLRRDKWM
                     YLLLLPGIIMTFIFKYVPMYGAVIAFKDYNPMLGILESPWVGLDHFREFISSPNFTLL
                     LSNTLKISIYGLLLGFFPPIILALSLNQIINKKIKQKIQLILYAPNFISVVIIVGMLF
                     LFLSANGPINGLVEKVTGQPIMFMSNPAYFRTIYILSGIWQGIGWSSILYTATLAGVS
                     TDLYEAANIDGANILQKIWHIDLPALRPVMVISFILAAGGIMNVGYEKAYLMQTSMNI
                     PASEIISTYVYKVGLQSGDYAYSAAVGLFNMIINVVLLLIVNNITKRLNDGQGL"
     gene            5743..6627
                     /locus_tag="SCL80_002068"
     CDS             5743..6627
                     /locus_tag="SCL80_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016608569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1107921.1"
                     /translation="MNSALSKIDRQILWLNRVIVTFLVVITIVPLIYVLVASFMDPAT
                     LINKGISFNPKDWTIQGYQRVFADDSILRGFVNSLFYSFSFSLLTVFITMITAYPLSR
                     PNLAGKKIIMTFFVITMFVGGGLIPTYLLVKNLGMLDSVWAIIVPGAINVWNIILART
                     YFSALPKELSEAATIDGANDLQILFKVMLPLAKPIMFVLFLYAFVGQWNSYFDAMIYI
                     KDPDLQPLQLVLRNILIQNQPSQDMVGANTAMAEMKQIAELIKYATIVISSLPLIIMY
                     PFFQKYFDKGVLVGSVKG"
     gene            6676..8283
                     /locus_tag="SCL80_002069"
     CDS             6676..8283
                     /locus_tag="SCL80_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1107922.1"
                     /translation="MKAKWMLPIVFTSVLILGACGGSKEKSKSSPDYELENVSFPLKE
                     KVSLKMMSQSAPLAPNDPNDKLIFQRLEEETNLHIDWTNYSSDFAEKRNLDIASGDLP
                     DAIFNAAAGDYDLLNWAESGVIVPIEDLIEDYMPNLKKIFDENPEYRQMSTAPDGHIY
                     SMPWIEELGEGKESIHTVNGMAWINKEWLDNLGLEMPQTTDQLMTVLEAFKTQDPNGN
                     GEADEIPFSFINNGGNEDLKFLMGAFGLGDNDDHLVVDNDGQVQFTANKEGYKEAIKY
                     FNEMNNKGLIDKEAFEQDWNTYVAKGKEHKYGVYFTWDKANITGADDSYDVLPVLAGP
                     DGTKHVTRTNGMGFSRDRFVITSANKNLELTARWIDKMYEPLQSVQNNWGTYGDEAQA
                     NIFELKDGMLKHLPLEGTAPGELRQKTEAGGPLAVLDSYYGTVTTMPDDAKWRLDLMH
                     DTYLPYITEDNIYPRVFLEKEETDRLAKIETDMWDYIYRKRAEWITGGKIDEEWEEYL
                     AELERVGLEEWIQIKQTGYDNFLKGAQ"
     gene            8284..9768
                     /locus_tag="SCL80_002070"
     CDS             8284..9768
                     /locus_tag="SCL80_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016621147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEG1107923.1"
                     /translation="MLVEETAILDKANRFIEMMRGKVNPQYRQNYHFSAPIGWINDPN
                     GFIYYKGHYHLFYQYYPYDSRWGPMHWGHARSSDLVHWEDLPVALAPDQPYDSGGCFS
                     GSAIERDGKLYLFYTGCQEIDGISHQHQCLAVSEDGIHFSKYSGNPVVAEKQLGENGL
                     IQEFRDPKVIVHNDGYYMVVATKSTQSTGRIVMFQSSDLYTWHFYSVLLEGTSEQGIM
                     WECPDLFHLDGRDVLIMSPIQIKKNGLEYHNISSTMACIGEVNWETGQMIVDNFHEMD
                     FGMDFYAPQTTRDPNGGRVMIAWMQMWDRTLPTHELGHLWAGSMSLPRELRVKKNRLV
                     QKPISTVYSHLVYQQGSENIAVTDTSVVFRNALSDSSYLHLVADLEKAEVFTIELLRS
                     KTDCLKLAYDTATQVFELDRQKLGRELTGAEEDPLNVRKVEVPLIDNQLVLEIFRDTS
                     SIEVFINGLEVMTATFYELEKARDILFHSIGESRIQAFETASVK"
     gene            9945..10937
                     /locus_tag="SCL80_002071"
     CDS             9945..10937
                     /locus_tag="SCL80_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005230454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1107924.1"
                     /translation="MKATMKDVAKLAGVGVGTVSRVLNNGSVKASTREKVEAAMTELN
                     YQPDFYARGLKMKHTNTVALILPTVWHPFFGEFAYHVETALERQNYKLYLCNSSGNSE
                     KEQEYIQMVSQNKVDGIIGITYSDIDEYISSNLPFVSIDRYFTEEVIYVTADNAAGGR
                     LAANELYRRGCQNVAYIGGYQETPNETKSRRKYFEIECRQLGISYKALDMLEPLDHLS
                     ERVTNFLLENPDIDGIFTINDPMALAVIDMLEKIGKNVPEDVQVIGFDGQKMTEGDPY
                     LVSTIAQPTKQMAEVAVAKLIAVINGETVEKRTVLPIEFGEGDTTKPLVRNDFA"
     gene            10989..11858
                     /locus_tag="SCL80_002072"
     CDS             10989..11858
                     /locus_tag="SCL80_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010748897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1107925.1"
                     /translation="MLYGGIEAGGTKFVCGIGNEQLQVIERKSFPTTTPEETMEQVFD
                     FFDKYKGNIASIGVGAFGPIDIQIESRTYGYITNTPKLAWQNYHFVGALENALSVPIY
                     WTTDVNAACYGEYMAGYGKGKNSVLYYTVGTGVGGGAVVDGKIVAGFSHPEMGHMLLQ
                     RHNEDQFEGICPFHHDCLEGLASGPAIEKRFGRKAQTISFDHPYWTIEADYLAQCVYN
                     TTLLYAPEVIILGGGVMKQPQLLELIRQSFKEKMGSYVDTPTLEEYLLTPRLADNAGL
                     IGCLALAIKKLNH"
     gene            complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCL80_002073"
     CDS             complement(12278..13771)
                     /gene="guaA"
                     /locus_tag="SCL80_002073"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEG1107926.1"
                     /translation="MITRRIREFGVFSELMSHRITAEEVKAMNPKGIILSGGPNSVYD
                     EKAFGIDEKIFDLGIPVLGICYGMQLITYKLGGKVKPAKNREYGKAELEITSAEAALF
                     QNTPEKQTVWMSHGDLVTQAPTDFDVVATSKDCPIASIQNAERKMYGIQFHAEVRHSE
                     YGNDLLRHFTLDVCQCTGDWTMDNFIDMEIAKIREQVGDKKVLLGLSGGVDSSVVGVL
                     LQKAIGDQLTCIFVDHVLLRKGEAEQVMESLGGKFGLNIIKVDAKERFLSKLAGVSDP
                     EEKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQGTLYTDVIESGTETAQTIKSHHNVG
                     GLPEDMQFKLIEPLNTLFKDEVRELGTQLGMPDSIVWRQPFPGPGLGIRVLGELTEEK
                     LEIVRESDAILREEIANAGLDRDIWQYFTVLPGIRSVGVMGDGRTYDYTVGIRAVTSI
                     DGMTADFARIPWEVLQKISVRIVNEVAHVNRIVYDITSKPPATVEWE"
BASE COUNT         4231 a         2337 c         2851 g         4391 t
ORIGIN      
        1 acctccagac ttcttgatag ctcaagtata tcttgaggtg catttgttag taaggtactg
       61 ttctatgggg cgcttacgtt gaaaaacgtt gatttatcgg cattccccat ccatattata
      121 tttacgtctt attcccactc aatatgagta gcctcgtaaa gaagctatta tatcagtgct
      181 ttttcagcct attcctcatt ttggtaggga aatggtaggg ttagattttt actgcttgat
      241 ttccattaaa gtttgcaact ttagattttg ttgcacaggt aaatttaatg tttcctgtca
      301 tttgttctgc aattccttca tcattacggc tccatagatg agcgtagtgc tttaaagtga
      361 tttcagggct agaatggcct aaccgttgcg acacgaccag aatgtctgcg ttaaactcat
      421 tgattaggta gctaacatga gaatgacgta atccttttcc ttgtattagg ggtaccttag
      481 ctagtttggc ataacgctga ataattcgtt gaacagttga acgatagagt gggaggtcag
      541 tgtagctaag aataaattgt tctacaccat gatttttttg aataactttc cactttttta
      601 gcacttcaat tgtatcatcg tccagagaaa tgatccgcaa gccgctttca gtttttgtgt
      661 aaggtttccg aataaaatct ttttgatttg tcatttccaa ggtgtgatga accctcaact
      721 ttttacgttt gaagtccacg tcattccaat taagtgctaa tccttcgctt acacgaacac
      781 cagtcatgta atatagccag atcattaaaa aacacatatg ttcgtagaaa tcatctttat
      841 aaatttttgc caaaacttct tcaaattctt cttttgtcca gtaaggaacg attgattttc
      901 cttttggaat tgcttttgtt cttttggaaa tattctcatt cagaaattgc agggtgaccg
      961 catagtctaa tgatttacga aacataccgt acataagtga actgtatgct tgtgagtatc
     1021 cgctatcgtt caataaccaa attcgataat tttcacaatc tcgcacattg atttcacgaa
     1081 gcttcttatc accgaaaaat tcagtaatct gaatcaatcc acactcacga gaactccaag
     1141 tacttttttg tacgctagct ttgtagtgtg gaacgtaaga ctgctccata aattctttgt
     1201 aagttaaatc ataattcgca aatccattta caaccatgta ttcatttttt atacgcatga
     1261 cttctttgtg tgcttcacga gctgatgaga atcgttttcc tagagaatct tttgttcctt
     1321 tcttctggat cctctttcca gtcacttgat ctgttcctaa ctcaactaga taagaaaagc
     1381 gaccattttt atcttgatac acacaagggt attttgtttt catagttatt cctcctgtga
     1441 aagaggaaca cctataattt cttcaattac ttgtcggggt actaccccaa ctcttttatt
     1501 attataatat ggatatccct gatttaccat tcttgttttt gcttgtcgga ttattgtggt
     1561 agcttgatgt gttttaaatc ctagagcctc taaatctttt cgacaaattg tatttttcat
     1621 tgtataaact cactttcaaa gaaaacacta tcattgggta atagtgttat ttctttattt
     1681 ttgtataatg aacggtactt tcttgaaaat ccatcacttg ttcaggactt cccaactcat
     1741 ttaaagctaa agcattgtaa aaaaatggac gtctaactat atattcgctt gtatatagta
     1801 tatcttgatt cgtggaatca tagggtttct ttaccatctt tttggtgaca gttggtagtt
     1861 tgagattccg ggacttgaaa aaagcttttt tcttatgctc tttcaattcc agctctttat
     1921 cgaaatattt gctgatatag cgcccgcgat tttcgatact gtctacatcg atcttgttga
     1981 tcttaacgaa accctgaccc caaatcgtag ttaaccggtt aaatgagata aaggggaaat
     2041 caaacaagat aatatggtag tggattgcac cgcgttgctg cttttcccac gtggcgagat
     2101 actttaaatt tgctttttta gttttataga ggtcataatt cagtcgtttg ataaattttt
     2161 taaattcatt atttgcatac tcaatgtctt gaatgttttc tttgaaagtg agagttaaaa
     2221 atttcgtttg attgtcaaaa ttcgtgtcta caaggcgggc aatctcaaat cgcatacttt
     2281 gataatgttt ctgtttgcgt tttaaactgt catattgtcc ttgagcggat aattcatcat
     2341 agttttttct tttttcaaga gtaatatcat tttcataaat ccaagctaat ttttgttttc
     2401 gttccagatt tacttcgttt tcgatcgttt tacgtttggg agtaatgatt gcttcataat
     2461 cataaatttc aatatacgtg ggagtttcaa tgattttctg attataagct tgcaaaaatc
     2521 atcactcctt gttgcgtatt cgattgtcaa agaaccagtc attttctcct gatgttgatc
     2581 tataaatcaa gttaaggggg aaggcctgcg tagcagccct tctagcctat ggggtgttgg
     2641 gcggtcttaa cggacagaaa cggctctgtg ggcgctgccc cgccgtttcg tcctaaccac
     2701 ccaacactcc atagtatagt tagttttgtg tagtggtacc ttgttttctg ggaatcaaag
     2761 ttgccgtatc agcggttgcc gataacccat aactgacaaa ttgtccattc gagcgtcccc
     2821 aaggtacaat caccaaattg gtaaatgtaa ttatggctaa accttgttgg gtaagttctt
     2881 ccggcgtgat cacaggatgt tcagatttga ctttgacact gattttttcg tagcgaagtt
     2941 ttggtaggat acattcgtag ctccaaccta gaatttcgcc ctccttttgc cagcgagtga
     3001 cattgacgac ttggtattct ttttcgagtg tttcttttgg taaactgaga tcggtaattt
     3061 ttattggcat cacaattctc cttttttata cgaaagttta tacgatttga ttaaaaaata
     3121 tagatggcca tctacgactt gtattatata cgagaactta tacgtagtca agagattttc
     3181 taaaaaaata tatacgattt tttatacgag tgttataatg aaaaaaatga taaggaggaa
     3241 tttgaatatg tttggtttag tacaacctaa taaagtgaag gtgggccaac gaatcaaaga
     3301 aataaaagac ggaatgtctc tctcttttac agaattaggg aatcgattag gtttaaaaaa
     3361 gccgacgatc agttcctacg ttcaaggcta tgcgttggct cctaagagtg tgatcaacca
     3421 attatctagt atcagcggaa aacctgtcgg ctggttttat tttgggcatg tggaagaata
     3481 tatccgagag tatttgtttc tcattggtca gaaaaaaata gtggacgatt atcctgaaat
     3541 cattgagaaa ataaagcaag aattttatac gggcgagttc aaaaatcctg cttgggagaa
     3601 tgagctaggc tatcctttgg aggaatttat ccaagaatgt tttgtagaat attcgagtga
     3661 aatcatgaag gactatgtcg tacagattac gaaagaggta cttgaagaat ccaaaacgct
     3721 aaacaaacta tcatcaaaag caaaagaaga agctgtcacg attctttcat ctgggatcat
     3781 tgactatatc gagatgagtg gtgagatcaa gtatggagag aaagatcgca ttgcgaaaat
     3841 ggtgaaagaa tcaataaaaa cctttgatgt gaaacaagac ctgacattta atgattccta
     3901 tttgattgga aagttgatca atattcttgg tacagatgaa gatacggagc tgtttctttc
     3961 ttctttatca atgatattta ctgataagca tttttccact cgatttggtg gggaagaatt
     4021 agtcgaaata tttcaatcga tgcgaccagc gttaatcaaa ctctatcggg agaaaacacc
     4081 agatgaattt tatgattggt ttgagaagta aaataagaaa agtggcttag attacaagtt
     4141 ggtgaaaaaa tttttttcag cataacatca gtaatctata aaggggttgt tgattatact
     4201 gtagaagtta agtactcaag agattttttg tacgtaaaca aagcaaaaaa gggtgcagtt
     4261 tcactagaaa atgtactctt ttttgtgatt tttcttagtt atagagtttt tttgaaactt
     4321 taaaaatgag aaaacatatt gattttaaag agtgtagaca tagcttcttt tgctaaatag
     4381 attaaactta ctggtgagtg gtcttgaaca tacttttaaa tcacatttta ctgagtttta
     4441 ttggatttta taattcattg gaaacctcgt acttaatctg tttttatcta tcgactagaa
     4501 tctgtatata ttccagctat caatgaggcc gcagaaaagg gtgcttaaaa ataatctgtt
     4561 gtttaaaata ttattatcag aatacttaga tacaaaattc tggcacaaag taaaaaaaat
     4621 attgacttaa aaaaatggat atggtaatct ataaacgaaa atagtggtaa cgtttccaaa
     4681 atgcatcttc gttaaatttt tattttttta aacgaaaatg gaaacgtttc taaaatgtat
     4741 tgggaggaga ttgaattgga aaagacattg ggaccaggtg gtgagataag ggcggtacaa
     4801 cgggataaaa aaaatatttc gaagtgggaa aaattaaaga aacagttgcg gcgtgataag
     4861 tggatgtact tgttgctctt accaggtatc atcatgacgt ttatctttaa gtatgtgcca
     4921 atgtacggtg ctgtgattgc atttaaagac tataatccta tgctaggaat tttggaaagt
     4981 ccttgggtag gactagatca ctttagggaa tttatcagtt caccaaattt tacgcttttg
     5041 ctgagcaaca cattaaaaat tagtatttat ggtttattgt taggtttttt tccaccaatt
     5101 attttagcac tgagcttgaa tcaaattatc aataaaaaaa taaaacagaa aatccagttg
     5161 attctatatg cgccgaattt tatctcagta gttattattg tggggatgct gtttttgttc
     5221 ctatccgcaa atggacctat taatgggtta gtagaaaagg ttacggggca accaattatg
     5281 ttcatgtcta atccagctta tttcagaacg atttatattt tatcgggaat ttggcaagga
     5341 atcggctggt catccattct ttacaccgca acgctggcgg gagtcagtac cgatttatat
     5401 gaggcagcaa atatcgatgg ggcaaatatt ttacaaaaga tttggcatat cgatttgcct
     5461 gcattgaggc ctgtgatggt gatcagcttc atcttggccg ccggcggtat tatgaatgta
     5521 ggatatgaaa aagcctactt gatgcaaact tcaatgaaca tccctgcctc agaaatcatt
     5581 tccacttatg tatacaaagt cggcttgcaa tcaggtgatt atgcctactc tgctgctgtt
     5641 ggtctgttca atatgattat taatgtcgtt ttattactga ttgtcaacaa tataaccaag
     5701 cggctaaacg atggtcaagg cttgtaatga aaggagaaaa ttatgaatag tgctttatca
     5761 aaaatcgacc gtcaaatctt atggttgaat cgagtgattg taactttttt agtcgtcatc
     5821 accattgttc cgctgattta tgtactggta gcttctttta tggacccagc tactttgatc
     5881 aataaaggta taagcttcaa tccgaaagat tggacaatac agggatatca acgggtattt
     5941 gcagatgatt ccattttacg cggttttgta aattcgttgt tttactcttt tagcttcagc
     6001 ttattgactg tatttattac gatgatcact gcgtatcctt tgtctcgacc taacttggcg
     6061 gggaaaaaga ttattatgac tttttttgtc attacaatgt tcgtgggtgg tggcttgata
     6121 cctacctatt tattggtaaa gaacttgggc atgttagata gtgtatgggc gattatcgta
     6181 ccaggtgcta taaatgtgtg gaatattatc ttagcccgta cctattttag tgctttgcca
     6241 aaagaacttt ctgaagctgc tactatcgat ggcgccaatg acttgcaaat actgttcaaa
     6301 gtcatgcttc cattagcgaa accaattatg ttcgtattgt ttttatatgc ctttgttggg
     6361 caatggaact catactttga tgccatgatt tacatcaaag atcccgactt acagccgttg
     6421 caactcgtat taagaaatat tttgatccaa aaccaacctt cacaagacat ggtgggagcc
     6481 aatactgcca tggcagaaat gaaacaaatt gctgagttga tcaaatacgc aacgatcgtc
     6541 atttctagtc tgccattaat cataatgtat cctttcttcc aaaaatattt tgataaaggt
     6601 gttttagttg gttctgttaa agggtaagta ttgttcacta tttgtatgaa taaaaattat
     6661 aaaggagatt ttaagatgaa agcaaaatgg atgctgccaa tcgtttttac ttccgtacta
     6721 attttggggg cttgtggagg ctcaaaagaa aagagtaaat caagccctga ttatgaacta
     6781 gaaaatgtct cttttccatt gaaagaaaaa gtatctttaa aaatgatgtc tcaaagtgcc
     6841 cctttggctc ccaatgatcc taatgacaaa ttgattttcc aacgattaga agaagagacc
     6901 aatctccata ttgattggac aaactacagt agtgattttg ccgaaaaaag aaatttggat
     6961 atcgctagtg gtgatttacc agatgccatc ttcaacgccg ctgcaggtga ctatgattta
     7021 ttaaactggg cggaaagtgg cgttattgta ccgattgaag atttgatcga agattatatg
     7081 ccaaatctga agaaaatttt tgatgaaaac cccgaatacc gacaaatgtc tactgcacct
     7141 gatggtcaca tttactctat gccttggatt gaagaattag gcgaaggaaa agagtcgatt
     7201 catacagtaa atgggatggc ttggatcaac aaggagtggt tggataattt gggtcttgaa
     7261 atgccgcaaa ccactgatca gttgatgact gtgttggagg ctttcaagac gcaagatcca
     7321 aatggtaatg gtgaagcaga tgaaattcct tttagcttta ttaacaatgg aggaaacgaa
     7381 gacttaaaat ttttaatggg ggcattcggt ctaggtgata atgatgacca cttagtcgtt
     7441 gataacgatg gtcaggtaca atttactgct aacaaagaag ggtataaaga agccatcaaa
     7501 tatttcaatg aaatgaataa taaaggtctt attgataaag aggctttcga acaagattgg
     7561 aatacgtatg ttgccaaagg gaaagaacat aagtatggcg tgtactttac ttgggataaa
     7621 gcaaatatca cgggtgcaga tgatagttat gacgtcttgc cagtcttagc gggaccagat
     7681 ggaacgaaac atgttactcg tacgaatggc atgggtttct caagagatcg tttcgtaatt
     7741 actagtgcga ataaaaactt ggaacttaca gctaggtgga tagataaaat gtatgaaccg
     7801 ttacagtctg tccaaaataa ctggggaacc tacggtgatg aagcacaggc gaatattttt
     7861 gaattaaagg atggtatgtt aaaacatttg cctttagagg ggacagcacc gggggaacta
     7921 agacaaaaaa ccgaagctgg aggaccatta gcagttcttg attcttatta cggcacagtc
     7981 actactatgc cagatgatgc gaagtggcgt ttagatttga tgcatgacac gtatttgcca
     8041 tatattacag aagacaatat ctatccacgt gtctttttag aaaaagaaga aacagatcga
     8101 ttagcaaaaa tcgaaacgga tatgtgggat tacatctatc gcaaaagagc agagtggatc
     8161 accggcggta aaatcgatga agaatgggaa gaataccttg ctgaattgga acgtgtaggt
     8221 ttggaagaat ggattcaaat aaaacaaact ggctatgata attttttgaa gggagcacaa
     8281 taagtgttag tagaagaaac ggcgattttg gataaagcaa atcgatttat tgaaatgatg
     8341 cgaggaaaag ttaaccctca gtatcgacaa aactatcatt tttccgcacc catcgggtgg
     8401 ataaatgacc ccaatgggtt tatatattac aaagggcatt accatctctt ctatcaatac
     8461 tatccttacg atagtcgatg ggggcccatg cattgggggc atgcgcgcag tagtgatttg
     8521 gtccattggg aagatctacc agtagcgtta gccccagatc aaccttatga cagcggcggg
     8581 tgtttctcag gaagtgcaat tgaaagagac ggaaaactat atttatttta tacaggctgt
     8641 caagaaattg atggtattag ccatcagcat caatgtttgg cggtctctga agatggtatt
     8701 cattttagca aatacagtgg taatcctgtg gtagcagaaa aacagctagg tgaaaatggg
     8761 ctaatacaag aatttcgtga tcctaaagta attgttcaca acgatggtta ctatatggta
     8821 gtagctacta aatctacaca gtctactggt agaatagtga tgtttcaatc gtcagatttg
     8881 tatacttggc acttttactc tgtgctgcta gaagggactt cagagcaagg aataatgtgg
     8941 gaatgtcctg atttattcca tttagacgga cgagatgtct taatcatgtc tccgatacaa
     9001 atcaagaaga acggactgga atatcataat atcagttcta ccatggcttg tattggtgag
     9061 gtaaactggg aaacaggtca gatgatagtt gataatttcc atgaaatgga ttttggaatg
     9121 gatttttatg caccacaaac aacaagagat cctaatggtg gacgggtgat gattgcttgg
     9181 atgcagatgt gggatcgtac attgcctaca catgagttag gccatctttg ggcaggctct
     9241 atgagtttgc cccgagagtt aagagtgaaa aaaaatcgat tggtacaaaa acccatctca
     9301 actgtatata gtcatttagt ttatcaacaa ggcagtgaaa atattgcagt gactgacaca
     9361 tcagtcgtat ttcgaaacgc gctgtctgat tcttcatatt tacatctagt cgcagatttg
     9421 gagaaagctg aagtttttac aattgaatta ctgcggtcta agacggattg tctcaaacta
     9481 gcctatgaca ctgctacaca ggtttttgaa ttggatcgtc agaaactagg aagagagctt
     9541 acaggggcgg aagaagaccc tctgaatgta cgaaaagttg aagtaccttt gattgataat
     9601 cagctcgttc ttgagatttt tagagatacc tcatcaattg aagtctttat taatgggtta
     9661 gaagttatga cggctacttt ctatgagctg gaaaaagctc gagatatctt gtttcattcg
     9721 atcggagaaa gtcgtattca agcttttgaa acagcgagtg tgaaataaat gtttgattgc
     9781 ttaggcgatg aatcaattat ttaataggtt ttgaaggata atgtttttag aaaagaacag
     9841 atagctagat ttcccatgga tttgctatta gtattttgaa aaaattagta atttatgaca
     9901 aaattcatta tactaaactt gtgcagaaat gtgagggatc aactatgaaa gcaacaatga
     9961 aagatgtcgc aaaattagca ggtgttggag taggaactgt ttcccgggta ttgaataacg
    10021 gttctgttaa agcttctact agagaaaaag ttgaagcagc aatgacagag ttaaactatc
    10081 aaccagattt ttatgcccgg gggttaaaga tgaagcatac gaacacggtt gctttgattc
    10141 tacccacggt atggcatcca ttttttggcg aatttgccta ccatgtagaa acagcattag
    10201 aaagacaaaa ctacaagctt tatttgtgta actccagtgg taatagtgaa aaagaacaag
    10261 aatatatcca gatggtcagc caaaataaag tagacggcat cattggaatt acttattcag
    10321 atatcgatga atacatttcg agtaatctgc cttttgttag tattgaccgt tatttcactg
    10381 aagaggtgat ttatgtgacc gctgacaatg cggccggtgg acgtttggct gcaaatgaac
    10441 tttatcgccg aggatgtcag aatgtagcat atatcggtgg gtaccaagaa acaccgaatg
    10501 aaacgaagag tcgtcgaaaa tattttgaaa tcgagtgccg tcaactgggg atttcctata
    10561 aagcgttaga tatgttagaa cctttagacc atctctcaga acgtgtaaca aattttttgt
    10621 tagagaatcc cgatatcgat gggattttta cgatcaacga tccaatggca ttagctgtca
    10681 ttgatatgct tgaaaaaata gggaaaaacg taccagagga tgtccaagtt atcggatttg
    10741 atggtcaaaa gatgacagaa ggagatcctt atctagtctc aacgattgcc caacctacaa
    10801 agcaaatggc ggaagtggca gtggcaaaat taattgctgt cattaatgga gagacagtag
    10861 aaaaacgtac cgtattgccg attgaatttg gcgaaggtga cacaacgaaa cctttagtga
    10921 gaaacgattt tgcttagtgc aaatacgttt ctttttttga aaatgagtga atgaaaggtg
    10981 tagataaaat gttatatggt ggaatagaag ccggtggaac aaagtttgtc tgtgggattg
    11041 gtaatgaaca attacaagtg atagaacgga aaagttttcc taccacaaca ccagaagaaa
    11101 cgatggagca agtgtttgat ttctttgata agtacaaagg aaatatcgca tcaattggtg
    11161 taggagcatt tgggcctatt gatattcaaa tcgaatcaag aacttacgga tatatcacca
    11221 acacaccgaa gttagcttgg caaaattatc attttgtagg agctttggaa aacgccttgt
    11281 cggttcccat ctattggact accgatgtaa atgctgcttg ttatggagaa tatatggcag
    11341 ggtatggaaa aggtaaaaat agtgttctgt attacaccgt tggtacaggt gttggtggtg
    11401 gtgctgtagt ggatgggaaa atagtcgcag gttttagtca tcctgaaatg ggacatatgc
    11461 tcttacaacg tcataatgaa gatcaatttg aaggaatctg tccttttcat catgattgtt
    11521 tggaagggtt agcatcagga cctgcgattg aaaaacgctt tggaagaaaa gcccagacta
    11581 tctcatttga tcatccttat tggacgattg aggcagatta tcttgctcag tgtgtgtata
    11641 acacaacgtt actatatgca ccggaagtaa taattttagg cgggggtgta atgaaacagc
    11701 cgcaactact cgagttgatt cgccaatcgt ttaaagaaaa aatgggttcc tacgtggata
    11761 cgcctactct cgaagaatat ctactaactc caaggcttgc tgacaatgcg ggcttgattg
    11821 gctgcctagc tttagcaata aagaaactca atcattagaa tagtatttat tagaactatt
    11881 cataacatac cggaagttta aaagggtatt ctgaaactta aatagtctca attcattctt
    11941 ctttcttgag aatctgagta gagactttta ttctgtttca aattaatata attacgaaat
    12001 gatattgagt acaagaaagt tctttttacc agatctttta aatcgaaaaa tctgatatat
    12061 atatttatag tactaaaaat ctgattagat aaaaagctaa ttcaatagga aaataaaggt
    12121 agtgaataaa agcaaagccc ttgttagtag ttaatctaac aagggctttg gtagggaaaa
    12181 tggtaggaaa ccatcttatt ttttacgaaa atcaggaact ttagtaattg aataatgttg
    12241 atttataggt attcctcatc catattatgt tggctcttta ttcccactcc actgttgcag
    12301 gtggtttgct ggtaatatca tacacgatac ggttaacgtg tgcgacttca tttacgatac
    12361 ggacactgat tttttgcaag acttcccaag ggatacgggc aaagtcagcg gtcatgccgt
    12421 caatagaagt gacagcacgg atacctacag tgtagtcata cgtacggcca tctcccataa
    12481 ccccaacaga gcgaatgcct ggtaggacgg tgaagtattg ccagatgtcg cgatctaaac
    12541 cggcattggc gatttcttcg cgcaagatag cgtctgattc acggacgatt tctaattttt
    12601 cttctgtcaa ttcaccaagg acacgaatac ctaagcctgg tccagggaat ggttggcgcc
    12661 aaacgatgct gtcaggcatg cctaattggg tacctaattc acgtacttcg tctttgaaca
    12721 atgtgttcaa tggctcgatc agcttgaatt gcatgtcttc aggtaatcca cctacattgt
    12781 gatgtgattt gatggtttgg gcagtttcag ttcctgattc gatcacatcg gtataaaggg
    12841 ttccttgtgc aaggaaagaa acgccttctt caccggcaag tttggttgct tcatcatcga
    12901 agacatatac aaattcattt ccaatgattt tacgtttttc ttctggatca gacacgccag
    12961 caagttttga taagaaacgt tctttggcat cgacttttat aatgttcaag ccaaattttc
    13021 cgccaagact ttccataact tgttctgctt cacctttacg caacaatacg tgatcaacga
    13081 agatacatgt taattgatcg ccgatcgctt tttgtaaaag aacaccaaca acagaggaat
    13141 ctactccgcc tgaaaggcct aataaaactt ttttgtcacc gacttgttca cgaatttttg
    13201 cgatctccat gtcgatgaag ttatccattg tccagtcacc agtacattgg cagacatcta
    13261 gtgtaaaatg gcgcaacaag tcatttccat attcggaatg gcgaacttct gcatggaatt
    13321 ggattccgta cattttgcgt tctgcatttt ggatcgatgc gatggggcaa tctttgctgg
    13381 tagcaacgac atcgaaatca gtcggtgctt gggtaactag gtcaccatga ctcatccaaa
    13441 cggtttgttt ttcaggagtg ttttggaaaa gggccgcttc tgcagaagtg atttccaatt
    13501 ctgcttttcc gtattcacgg tttttggctg gtttaacttt tccgcctaat ttataagtaa
    13561 tcaactgcat accgtaacag attcctaaaa ctgggatccc caaatcaaaa atcttttcat
    13621 caataccaaa ggccttctca tcataaacac tgtttggtcc gccagaaagg atgattcctt
    13681 tagggttcat tgcctttact tcttctgcgg taatgcggtg gctcatcaac tctgagaaaa
    13741 caccgaactc acgaatacgg cgagtgatca gttggttata ctgactaccg taatcaagta
    13801 cgataatttt
//
LOCUS       DARBEC010000072        13757 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.074, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000072 DARBEC010000000
VERSION     DARBEC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.074"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            206..544
                     /locus_tag="SCL80_002074"
     CDS             206..544
                     /locus_tag="SCL80_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107927.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(749..1324)
                     /locus_tag="SCL80_002075"
     CDS             complement(749..1324)
                     /locus_tag="SCL80_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEG1107928.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGF
                     YEWNEEKDKFMFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRMPLVL
                     TEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(2056..2637)
                     /locus_tag="SCL80_002076"
     CDS             complement(2056..2637)
                     /locus_tag="SCL80_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1107929.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(2820..3446)
                     /locus_tag="SCL80_002077"
     CDS             complement(2820..3446)
                     /locus_tag="SCL80_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1107930.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(3468..4799)
                     /locus_tag="SCL80_002078"
     CDS             complement(3468..4799)
                     /locus_tag="SCL80_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEG1107931.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(4954..5355)
                     /locus_tag="SCL80_002079"
     CDS             complement(4954..5355)
                     /locus_tag="SCL80_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEG1107932.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(5441..5647)
                     /locus_tag="SCL80_002080"
     CDS             complement(5441..5647)
                     /locus_tag="SCL80_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107933.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(5742..7244)
                     /locus_tag="SCL80_002081"
     CDS             complement(5742..7244)
                     /locus_tag="SCL80_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEG1107934.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCL80_002082"
     CDS             complement(7262..7717)
                     /gene="argR"
                     /locus_tag="SCL80_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEG1107935.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(7815..8432)
                     /locus_tag="SCL80_002083"
     CDS             complement(7815..8432)
                     /locus_tag="SCL80_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEG1107936.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKPFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQLVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            8653..10026
                     /locus_tag="SCL80_002084"
     CDS             8653..10026
                     /locus_tag="SCL80_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107937.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLILSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            10101..11213
                     /locus_tag="SCL80_002085"
     CDS             10101..11213
                     /locus_tag="SCL80_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEG1107938.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWSTEDYQKDISTYITKNPS"
     gene            complement(11285..12922)
                     /locus_tag="SCL80_002086"
     CDS             complement(11285..12922)
                     /locus_tag="SCL80_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEG1107939.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(13090..13698)
                     /locus_tag="SCL80_002087"
     CDS             complement(13090..13698)
                     /locus_tag="SCL80_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1107940.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
BASE COUNT         3711 a         2892 c         2464 g         4690 t
ORIGIN      
        1 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
       61 ttaagaaact aaaaaaataa tccaagaaac attgaaccta tggatccaaa agctacccaa
      121 atactatagc taaccccccg ctaccttcaa ttttgcagat aaaacgcttt gggagaaaat
      181 ttcaaagaca acaggccatc ctatcatgcc cgattataaa caaaaagctg gctattcctt
      241 ttgctctgac aaatttcaat tgggaaagtt cacgaaacta gcaggtgaag aagtttcaac
      301 tgttcggatc aaagaatgcc cgcttcaaat agaaacattg gttcaacgaa tttcaactag
      361 acaagacttt gctattgtag aacgtgagat cgtagctatt tttgtagaag aagggatttt
      421 acatgatcca actcatatca atgtggataa atggcaacct cttatctata aattcagaga
      481 atatacaact gccaaccagt cgttaacttt gaattttaga ttccaagaat tcaaagagtc
      541 ataatgataa acatcgttag gtataaataa aaaagactcg cagcacgagt aaaaaacaaa
      601 tagaagctat acattagaaa ggtcgactgt gaccctatag atgcagtata tgcagcttat
      661 gagcatttct tctgttctct ttgtttttgg gatctgtgct gaagtctttt tcgttttttg
      721 ccgctctttc tttgaaaaat cgattatgtt ataccttctc cattactaat tcaggcatct
      781 gctgagtaat gatttttcgc gcaaaagaaa tatctgttac ccaaggttcg atttgttctt
      841 cagtcaagac aagcggcatt cggtcatgga ttggtgcgac tgtgcggtta ggttcagtag
      901 tcattatgat tgattctgcc attttttcat cctgatggct acgataaaaa ccgccaatat
      961 acactatttt ttccttattt gaaaacataa atttgtcttt ttcttcattc cactcataaa
     1021 agccactcat tgggaaaaca caacgatttt gcaaaaatgg ttgatgaaag gtcttttttt
     1081 cttctactgt ctctgcacgg gcattaatca tcaattgtcc ttttttaaat ccggtaaatc
     1141 cccacttcat gatttccgga gtgaggatgt tttcttcctc ttttcttaac gtcacaacaa
     1201 gatttgacgg aaaaacttct ccagctgcta attctgctgc ctgcttcggt tgtttcgtct
     1261 gggcttctcg ataataattc tgaaggagct ctgactctag atcaaaaaaa taacgaccac
     1321 acatcctaat ccctcccaat ctttttttct atcatacact gcaacaagaa gttcgcacca
     1381 acaaaacgct ttcaattttt gtagaactaa aaaagttgtg acaagtgaca aactctccag
     1441 tgaatggtat tctcctgcca actcctcggt cacaagtcac aatattcaac aaacatgaga
     1501 agctgctttt tgaaattgtt ccttgtgact cggagtgaaa cggcaggctt acgacctctg
     1561 ggtaaacggt gttcaaaagc cagtttctcg gtattaagcc aaatttatgc aaaaatgtag
     1621 aaagccattt tcacaaaatt tttcttaata ctcgaaacta agcgactttt tcacaacctc
     1681 ttccttaaac ggtgttcaaa agcttgcatc tgcggtaata agcccaacca aacgaaaaat
     1741 atgagcagct atttttattt ggctgttctt attacttgat gcagggcagc tttttcacaa
     1801 cctctccact aaacggtgtt caatcagcta accttcggta ttagctcaaa aaacggcaaa
     1861 atatgaggaa ctattttgcc gtttgtttct ctaatactca ggtcaaagcg ctgatttcac
     1921 aacctctaat acacggtgtt ccagaagtag cttcttcgaa aaataagccg aaatttcaca
     1981 aaaatttgag aagcaatttt cgtaaattcc ttcttatttc ttgaagctga ccacttctgt
     2041 cacaacctct aattattaaa gaaccttttg cgaagttaac aatctttctt tgatcaattg
     2101 aaaattctgc ctaagtagtg atagatcctg tgtgactttc aacaataaga tacttccctg
     2161 gatttcagca atcaacgctt tggcttgacg gtctgcttct tcatcggaca gtttttcttc
     2221 agaaagtgcc aggatatttt gtttcgtata cttgacccat tgattgacta attctagtag
     2281 caacagacgg tattcttcat ccactgtact caactcaata atcaaattac ctacaggaca
     2341 gccatgataa gtgatctgtg tctcatgatc agcgaaaatc cagtccaaca tttcttctaa
     2401 tgcttcttct ggctgttttc cagcatttaa gatgtcttcg aacaattctg tctgccatac
     2461 gtccatatgc gcatggatca ccgctagaca aacttctttt tttgaagaaa agtaataata
     2521 aaattgacct tttcctgtat cagaagctgc tagaatatct cggattgacg tttcatgata
     2581 gcctttttta taaataagat ctttggtcgc ttccaagatc ttttcctttg ttttcaatga
     2641 tattctcctt tttcatttct catagtccta tattatacag taaattcatc attttttctt
     2701 cttaatcgtt taaaaaagac tgtgccaaat tatctgacac agccctcact ataaaactgt
     2761 tctcatatag ctagatgttc attgattcaa gaactgcgag gacaaaagca attcctatct
     2821 tatgatagtt gctctaatac ttcctctgtt gtcatcactt gatctgcaat aaagccaaag
     2881 ttcgtcaacg ctgcatccat tgcttcttgg ccaggagcac caattgcatc tgtcgctacc
     2941 acaacattga atccactctc taccaattca cgcatatggg aatcgctaca caagtttccg
     3001 ttcattccac caagaattac tgtgtcgatt ccattttttc tcaattggag cgccaaatca
     3061 ttgctttctg gtccaaagat tttatgagga gagctgatga tcgtattttc atccaaataa
     3121 ggtttcaatt cttcgataaa atcggctcct gaaccttctt ccactgattc atattgactt
     3181 tttctagcaa acatctgctg ctcaagcatc atatgttctc ctgcaccgcc aaattgccat
     3241 ttatggtcat gtgggtaaaa ataatgagga gaaataaaga gtttgtatcc tttttctttt
     3301 gcttgtttta ccatttcaat catattttcg atcgtattgt atttttctaa gatatctttt
     3361 gtcaaaccaa agccaccgcc tgctggtttc aagaactcga cttgcgggtc agtgagaact
     3421 actgctgtat ttttattatt gaacatggtg gattctccat tcgttattta ttttaataca
     3481 tcatttagag cttcactcat tgtcggatga gaatagattt gatctctcaa taacgtataa
     3541 ggaagttttc cattcatagc tagcgaaatc aagttgatga cttcatgaga atcttccgca
     3601 tagatacttg cccctagaat caagtcagtt tcagggtcga ctaaaacttt gaatacacct
     3661 ttaggatctt ctaatacttg tgctttaggg acaccgcttg tcatatactt gaataatttg
     3721 taattgacgc cttgtgcttt ggcttgtttt tctgttaaac ctacattgga taaagtcgga
     3781 gtgataaata cactgtaagg cacagctttc cggttattcg ttgttcgttt attctcgccc
     3841 ttcaactgat ccataatgat tcgataatca tctagagaga tataagtgaa ctgcaatccg
     3901 cctttgacgt ctccaatcgc ccaaatgttg tctgcatctg tacgaaggta ttcatccaca
     3961 gcgattgctc cgcgatctgt tagtttaaca tctgtatttt ctaatccaag tgatgctgta
     4021 cttggttttc ttccagttgc agccaaaact tttgacgctt ggattgttac ttcttggcca
     4081 tcttctgtaa aggtgacggc agctgttgat tcttggtctg ctacttgatc tacagataca
     4141 cctagatgga attcgattcc cgcatttgtc atgtcttcta aaatcatttg cgcgatatct
     4201 tcatcttcac gtggaagaaa ttcagaatgg gcatcaagaa cgacaacttt agatccgtat
     4261 tcattgaaca ttgatgcaaa ttccaagcca atatatccag ccccgatgat cactaatttt
     4321 tccggtaatt gttcctgatc catcgcttgc gtagaatcaa tcaagtagcg acttgtattc
     4381 aatcctttga ttggtaaaat gattggttca gaaccagtat tgatgaaaat ccgttcgcct
     4441 tcaacaatca ctttttcacc attattcgtc acttcgattt gatggttaga aagaaaacgt
     4501 gctgtaccgt ccatcactgt cccagttgcc tcatcgctga tcatatgata atttttggcg
     4561 cgaagcatgc ccgtcaattt ttctttccga gcaacagcct ctgtaaagtc gattcctctt
     4621 tctccattga aaatcaatga tttagaagga atacagccga tgttgataca tgttccgcca
     4681 tacatacgtg ttgacttttc aacgactaaa accgattcac cttttgaagc taacgccttc
     4741 gctaaagtct taccgccctt tccaaaaccg atcacaatat tttgatattt ttccatcatt
     4801 tttccctcgc ttctttttta cctaccgatt ggtacaaaaa tatgataacc gataattgtc
     4861 tattctccaa atcatctgct ttatttgtca aaaaaaacca gacaaaaagc ggtcctttca
     4921 cgtttttagt ctggttcgat ttcctttatt cttctaaaca atcgttactt ctacatcgat
     4981 atttcctcta gttgctttgg agtaagggca gaattcatgc gcatcttctg caattttttg
     5041 ggcttcttct tcgctgatgc catcgatatg cacttctaat actacaccga tctgatagcc
     5101 gccatcatct aaattataaa gagagacacg tgcttttact gtggactctg ctgtcattcc
     5161 tgcttctttg atcatatgtt gtaccgcccc attgaaacaa gaagcataac ctgctgcaaa
     5221 taattgttct ggatttgttc cttctgcatg gattcctgga ggagtgattt tcatatgcat
     5281 cgagccattt ggtgcctcga cttctccatt gcgcccgcca tggttgatca ttgctgtttc
     5341 gtatgctttt ttcattgtac tgctcctttc gattgaccat ttgctacttg gctataatta
     5401 tagtgtatga caagtaaggc gctttctaca aaaattctgc ttattcgaat accagaatgc
     5461 cgtcttctac tatttttcca aaaaacaaaa aaatactact cgtgaacaac agcgtcatat
     5521 ttgttccaaa ataaatagca atcttttcta cataatcttt tatatgattg attttaagat
     5581 acatatatac cagaaagttg accccataaa ccaatactgt taaaaaaata accatattcg
     5641 ttaccatttt ttcctccttt tgctggtctc cctaccagca ctcgatttaa ccataccact
     5701 ttttttgccg tccaagcaac tagcctttca aaaagactgg tttattctgc tgttaatgat
     5761 tcaagatcag aaaaatgtcc gaataatttt gctaatttta ctaacgccaa cttgttcttt
     5821 ccgaattcat cattttctat ctgtaatact agtaacggct catgtaagct taaacggatc
     5881 aaaaaccagc cgtttccgta tggctcttct agatcatatc gaataccttc ttcattttcc
     5941 gggtcgaagt tcatcccttt ttcttcttcc aacagtcgct tcatttcttc taatatcttg
     6001 tttccttgtt cgcgtggttc atcgctgaac aattttaatc ggatttcttg tacttctacg
     6061 ggctgtttca actctttgat ttgttcatct aatgtgatac cttttctttt taattctggc
     6121 aaaagcatca ggatctttgc gatgacatat gcaccatcat ccagaaaata gttttccttg
     6181 aatgcagcgt gtccgcttgt ttcgatggct agctgtgtat ctttcccaag acggttaagt
     6241 tctaatgctt tattgattac atttcgatag ccggaaatat aacgaacttg gatgcctcct
     6301 ttttcttcaa tgaatctttg tagatgagaa gaagtagggg aattcgtcac gattcttgtt
     6361 cctggatgct cttttaatac gattgtactt aatactgcga tcaaattatt tcgattgatc
     6421 actgttccag aagcactgac aactgcagaa cgatccacat ctgtatcgaa aatgatccca
     6481 agatctgctt catttgctaa aacagcctgc ttgatacttt ccatcgcttc tttattatca
     6541 ggatttggga tatgattagg aaatcggcca tctggttcca aaaattggga acctgttgaa
     6601 tccgcaccta agctattcaa tactttttca gcaaagaatc caccagcacc atttccggca
     6661 tcaacaatga ttttccaacc atttagtggt ttgctttcta gtgttccaat ccccttttta
     6721 atttttgtga ctaaatcttg cgcataaggt gtcaaaaggt ctgctttttc tacgcttcca
     6781 gtttgttcgc tttttatttt ttgctgctgt gataggataa aagcaatatc ttctttttct
     6841 gctcctcctt ctgaggtaaa gaattttatt ccattataat aatagggaag atggctagca
     6901 gtcagcataa ccgcagcatc gcacgagaac tgttcaaatt gagtggacat aaacatagca
     6961 ggcgtagtag ctaattcgaa atcgatgaca tgtatccctt gttctttcag actttggatc
     7021 aacgcctctt tcagtgctgg tccgcttatc ctactgtcat gaccaatccc aatcgtccat
     7081 tttttatttg gatcggttga tttcttcttt ttctttatcc agtttgctag tccccagccg
     7141 atctttttta ccatgtctgt cgtcaagttt acttcatatc cttcaactgg taatgcgatt
     7201 cctcgaatat ccgatccatt ttgcagcgct ttcatatctt tcatataaaa gtcctcctca
     7261 tttattccct cattttttcc agcttttctg caaatcgctg tgcttcttct tctgagcggc
     7321 aaataataat gaatgtatct acaccggcca tcgttccgac aacttcaggg taagccactt
     7381 catctagcca attcgttacc acatcggcac catttcgttc agtcaatacg ataaccatga
     7441 actgaacaga ctggatacgc aaaacctcgc gtctaattgc tgtacgcaaa cgttcttcat
     7501 ttaaagaaac agatggctgg ttaaataatg tatagcgtac tattttgttt tcattttgac
     7561 tttttactaa cttcatttct cgaatgtctc gagaaatcgt tgcttgagtc acatcggcgc
     7621 ctgcttcatg cagccgcgca agcagctctt cctgtgtccc aataggttgt tctgtaatga
     7681 tctggcggat cagtgcttgt cgctgtttct ttttcatatt agacacctct caatgttgat
     7741 tatagcaaaa gataagtaat atttcttcat attttggtat tttttacttt tattttttgt
     7801 tttcattcag gttgctaacc tttttctatt ttaacacgca aaatcgtctt cattttttca
     7861 ggtgctgatt ttcttggatc gctgatgtaa atctctttgt gttctttaga agctctttca
     7921 tagccttctt ttgcgatgaa tgcttctaat ttctcaaacg ttgctggttc ttcggcatag
     7981 ggcccgacgt gtaagatttg ggcacaaaga ccttcttcca tctttttgaa tactagttgt
     8041 tctttcaagt cttccggaag tttgctagaa cgctgaatgg cttcttgtgc tatcgcttct
     8101 gtaacaaagt caggctgtcg gatcatcaat tgataagaaa aatgttcttt tttcatcact
     8161 ggttcatgca aatacttttc ttgcagtccc cattgtcctt ccagtggata gacagtatac
     8221 atctgataat caggaatcag ccaatttttt ttaggcgaca tgcgtattgc atagctgact
     8281 gcatatagac aggctactct tcgtttaaag tcttcctgat tcgggtcgcc gatccctctg
     8341 atgctaaaga atggtttttt aggaacctct agtatttgcg ggcttttttt catactatag
     8401 ctcttgtctt tacgccattc aattttttcc atttttcttc ctccgcgaaa ttccatttag
     8461 ttatttatta tcatacctct ttttgagtga cttggctaat cgtgttgaca ctatttttca
     8521 gtttgtgcta gaataagtga agtaaataaa tcgtacagat aacacgtttc ggcgtgtcga
     8581 gagagaggcg gtttctattt ttataataga aatcactctc tttttttacg aaatttttta
     8641 gaggtgatgt aaatgacaaa acaaaaacag ctgcttttag tacctgcagt aattgcaaca
     8701 gggatgatgt cttttgcggg cgtattgatc gagacggcca tgaatgtaac tttcccaact
     8761 cttatcagac agtttgattt aacgactgca caagtccaat gggtaacaac gatttatctt
     8821 ttgatgattt cgatcattgt tccgctgtct acttatttga atcgcaattt tagtctcaaa
     8881 aaactctttt tagcttcaaa tctgcttttc ttggcaggta taatgatcga tttcttctct
     8941 cctaccttca tggtcctatt gtttggtcga ttgctccaag gtgcagcgac aggtatcgct
     9001 cttccgttga tgttccatat tatcttgaca tttgcccccc tcaatcgacg aggcgcgatg
     9061 atgggtgtcg gtactttgac aacggccatt gctccagcaa ttggccccac ttatggaggc
     9121 attatgactt cacattttac atggaatcat atcttcctgt ttcttgtgcc cgttttgatt
     9181 ctttctttga ttatcggctg gtttgctatt cccgagatgg cagtacaaaa aagtggttat
     9241 ttggattgga taagcgtttt aggaatttgt cttttgttta gtggaagtct aacatttttg
     9301 agtacgttgg gaacagtttc cagctggatt gctttaatca tcggcggtat tggctggttc
     9361 cttttttact atcgaagtaa aaaagtggag catccactga tccgactgtc tattctttca
     9421 aataaaacat tccgcttgtt tttgattggc ttcttagttt atcagttttt attacttggt
     9481 gtatcttttg tcttgccgaa tttccttcag atcgttcaag gaagtagtcc ttttgtcgct
     9541 ggattagcga tgcttcccgg cgcagcggtt ggtgctttgc tttctccttt ttccgggaaa
     9601 atgctggatc aatatggtcc gaagaagcca atttttgctg ggctgattct ttctttgatt
     9661 ggttggctag cattattcct actcatagga cacgcttcag tgggtgtatt gattgcttgt
     9721 catgtgtttt atatgatcgg tatcggatta tcttacagca atatgatgac caccggaatg
     9781 aatgcactga atgaagaact acaaggagac gggaacgcaa tgttcaatac cttgcagcag
     9841 ttttctggcg ctgtggctac ttcattagta gcggtcatta tcaattacgt ccagcatcat
     9901 acttcccata actatgaagt gtctactaca ttaggatcaa aagcagcatt aggcgtcttg
     9961 ttgctgttgc ttctcgtaag ctttgctcga ttcgcctatt atctgttctt tgctaaaaaa
    10021 gcgtagattc catatacggc aggtgttgca tctgccgttt ttttgtgtga aaatggttgt
    10081 ttgtaaaagg aggctttttt atggaaaaaa ttgccattat cggtggcggg atcattggta
    10141 tgactctagc cagttactta gatacatcaa aattcaatgt tgctttattt gatgaaggga
    10201 caggacaggc aactaaagct agcgctggga ttatctcccc ttggttgtcg aaacgcagaa
    10261 acaaacaatg gtatcgtctg gctaaagacg gcgctgcttt tttccctaaa cttataaaag
    10321 atttccaact tgatcaaacg atttacgcac aatcagggac acttttgctt cgacctgaaa
    10381 aagatttagc tgaattggct gttcttgccg agaaacgaaa agaagaagcg ccagaaattg
    10441 gaaacatcga atggctttca cctgaacaga catctcaagc acttccgtta ttgaaaccag
    10501 cagtttcact gcgtatcagt ggtggtggtc gtctggatgg aaaagcatat ttagaacatc
    10561 ttggtcagcg tttagcacaa aaacaaatgg agcagatcca ggcaaaagtc actttgcata
    10621 agaagagcga ttcttggctc atcaagggac cgtttggaga atacacagct gatcgtgtaa
    10681 ttttgacacc tggcccggca cttaagcctt tattacaaga attaggctat gctgcagata
    10741 tacgtccgca aaaagggcag ctcttagcat ttcagacatc ttataagatg agtcaagact
    10801 ggcctgttgc tatgcttgac ggagaagctg atttcattcc tttcatggat ggaaagatcc
    10861 ttttaggtgc tacccatgaa aacgatcaag gctgggattt ggctcctact agcgaagctt
    10921 ataagggatt gactaagagt acagaacagt tcttgactga tccagatcag ataaaaaact
    10981 ggccatacga gttccgtgta ggtacccgtg cttatacgtc agatttttcc ccatttttcg
    11041 gcgtactgcc agatgatcca agcttactgg ctgctagcgg tctaggctct tctggcttga
    11101 ccacaggtcc ttatatcggc tatctattag ccaacttctt aaatactggt cattggtcta
    11161 cggaagatta tcaaaaggac atctccacct atattacgaa aaatccatca tagaaaaaca
    11221 gctttttcaa gtaaggatcc cgagcgatat tgcgcatgga ttacttcttg aaaaagctgt
    11281 tattttagtt caatttctga atcaaattct ccaaactttc ttttgcttca tctggcaaga
    11341 gatcttttcc tatctgagct tcaatgaatc gtaaacgaat atcaagtcgt tgtttcacca
    11401 gatttttata ggacgtatcc tcaaaagaaa caggaaaatc tggtataggt aaatagtgga
    11461 tgtcttcaaa tggaccaaac gacttgaatt ctgccgtttg ttgcacgatt ctttcaacga
    11521 ttgacaatgt cgtttcaggt ttgatttttt tatcatcgaa atatccagta ttcaggacat
    11581 agcaggctgt ttgctgtttc aaaaacaact ctcgaaaagc ttgatagtct tccctaagag
    11641 gatagacacg gaaaggatta gcaaatggtt caatgactag ctcatctagc tgtctttttg
    11701 tagtgatatt ttccgcgtct gatcgttttg ttgctaatgt tgcaccaaaa gacgctgcta
    11761 aaactgggtc attgatcttg ataattggcg gaagcgaatc atctttcatg atccaaaaaa
    11821 cagcatcgat cttttcgcta agatgatcga ctcggtcggg tgtcgcataa cgtgatttga
    11881 ctgtccgccc atttccgttt cgtatatctc ctgttactag gactttttct cctttacttg
    11941 tacatgtcac gcccacattc tgacaagtaa ggaaagaaga aatggccgga tcatcagacg
    12001 gatagtcttg tgttttatca aagtaagaag gttccaaggc agtagcaaaa ccattttttc
    12061 gagaaataac gaaagcatcg tcatgcaaga tcgaaacaga gtgaccttcc gtatgttttg
    12121 ctaaggtaat cgttgatttt ccagaaccgg acaacccaaa tacagccatc gtataggtct
    12181 ggtcattact agcatattgt ttcatcccac cgtggcaggc aataaagcca ttgcgatttc
    12241 ccaatgccca agcaagcgtc aaagtcgctt tttttaattc tccaaagtaa cgtaatccta
    12301 gcacagccgc tgtattatgg agagcatcaa acaaaatgag tccatttgga aatgtttcat
    12361 gtgtccattc gggatcacaa aagatataaa gatctccctc atcaaaggaa acagactttt
    12421 gataagtctc taagatcttc gaattcgctg gctgaaaatt cagcaaataa gaatacaaat
    12481 tgttctcaaa tccttctgga atcagcaaat gactttgcac agaaaattct tcagataggc
    12541 caacgattac ttttgtatgg taaaactggc gcttgcgact ttgatacaca gcttctctta
    12601 aaattttctc gtatttgctt tgttccttgt ctaagtcctc aaagatccgc cgagcacttg
    12661 ctgttcttcc cacaatgctt ccatcattga atactagtgt cctaacatca gctggtaaat
    12721 ctagttcttc agcatgtgag attgttaaat ctgtgatgat cgttcctgga ctcccttttg
    12781 ctagttgata agctgtcttc ctgtcaaaaa cttcatgaac attattaccg taaaatgtcg
    12841 tttctacaat cgatcgaata ctggataaca gtggatttcc acttgtgatt tcgtttaatt
    12901 cgagactctc ttttgtactc atggctcctc ctcattccct aaacaaaaaa acgttttctt
    12961 tattatacat tattcgagtt tttatttgta ctgctgcgct gtgggaagca aacgagttat
    13021 ttttcacaca aaaaaaccga gaacttgaat aaacaagttt ctcggtttca tcattttcct
    13081 tttatcggct tagtaccagc cgtttgctaa ccagaatgtt ttagcagcat cccatgaacc
    13141 ataacgagat gttacatatt gttctgctac acgttcttga ttggcagctg aatagtcgcc
    13201 gtttaagtat gaagcatcta attgataacg gccgatgtaa cgtccgttag tagctgtgta
    13261 tgaaccgcct gattcttttt gtgcgatcca ttcttttgca gagcttgaag ttggcgctgt
    13321 tgcagcagct ggggcagcag gagctacttc aactgtttca acaggtgcaa cgtatgtatg
    13381 ttcttgcgtt gctgtttcag ttgtagatgc tgtattttct gttgttttgt tttctgttgt
    13441 tgctgaagaa tcatttgaag ttgggattgt taattgttca ccaacataaa tgcggttgat
    13501 gtttgcgatc gaattttttt cagcaattgc atcgatcatt gagttgtctc ctgcaaattt
    13561 ttgtgagatc ttagataatg aatcacctga ttttactgta taaacttcat cagcgtgagc
    13621 atttgtagtt cctacaaaca ttgcagtagc accagcagca aaagttgtac ctaaaacaat
    13681 tttcttaaat gaattcatat taaactcctt tttttctccg ttttttattc tttcgacgct
    13741 attaacttta tcattta
//
LOCUS       DARBEC010000073        13683 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.075, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000073 DARBEC010000000
VERSION     DARBEC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13683)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13683)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13683
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.075"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1404
                     /locus_tag="SCL80_002088"
     CDS             1..1404
                     /locus_tag="SCL80_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEG1107941.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1753..2385
                     /locus_tag="SCL80_002089"
     CDS             1753..2385
                     /locus_tag="SCL80_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEG1107942.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLSGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            2973..4412
                     /locus_tag="SCL80_002090"
     CDS             2973..4412
                     /locus_tag="SCL80_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1107943.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4707..4925
                     /locus_tag="SCL80_002091"
                     /pseudo
     CDS             4707..4925
                     /locus_tag="SCL80_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5174..5389
                     /locus_tag="SCL80_002092"
     CDS             5174..5389
                     /locus_tag="SCL80_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107944.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5606..5862)
                     /locus_tag="SCL80_002093"
                     /pseudo
     CDS             complement(5606..5862)
                     /locus_tag="SCL80_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6049..6960)
                     /locus_tag="SCL80_002094"
     CDS             complement(6049..6960)
                     /locus_tag="SCL80_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1107945.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(6979..7983)
                     /locus_tag="SCL80_002095"
     CDS             complement(6979..7983)
                     /locus_tag="SCL80_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1107946.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(7980..10103)
                     /locus_tag="SCL80_002096"
     CDS             complement(7980..10103)
                     /locus_tag="SCL80_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1107947.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10108..12555)
                     /locus_tag="SCL80_002097"
     CDS             complement(10108..12555)
                     /locus_tag="SCL80_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1107948.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLYGRVGVVQVHPVFE
                     ELFHAFDTRPPVQERME"
     gene            complement(12542..12931)
                     /locus_tag="SCL80_002098"
     CDS             complement(12542..12931)
                     /locus_tag="SCL80_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1107949.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            complement(12991..13494)
                     /locus_tag="SCL80_002099"
     CDS             complement(12991..13494)
                     /locus_tag="SCL80_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1107950.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            complement(13507..13668)
                     /locus_tag="SCL80_002100"
     CDS             complement(13507..13668)
                     /locus_tag="SCL80_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107951.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
BASE COUNT         4204 a         2699 c         2501 g         4279 t
ORIGIN      
        1 atgaaaaaat ctttaaaact tgcatcagca gttatattaa tgtcccaact ccttatagca
       61 acacctttcc atgtgttggc aagcgaaaat tcaacataca aaattgcaaa agaaagaacc
      121 gagacgactg aaatgagtaa ttccgatgta acagcagaga gtagtgaact tttgacaact
      181 tcatcaagtg aaagtagtac gacttcaaat gccgagtctt caacaacttc atcaagtgaa
      241 agtagtacga cttcaaatgc cgagtcttca acaacttcgt caagtgaaag tagtacgatt
      301 tcaagtaccg aatcttcaac aacttcaagt agtacaagtg aaataacttc ttcgaaagtg
      361 acaaaaccaa accatataga atcttcaaaa aaggaagcgc caaaaacgga aggacaaaca
      421 aaaacttttt cgacaccaca gcaaatagaa tcaacagaat ctttccaatc acaaccaata
      481 ctatctggta atgaagaaat tcattttgac aaaaatgaat ctgtagaaaa ttttattatg
      541 aaaattggtg agtctgcgag aaaaattggt cagaaatatg atttatatgc ttctgttatg
      601 attgcacaag cgatcttaga atctgccagt ggtcaaagcc agttagctca ggcaccgaat
      661 tacaatttat ttgggataaa aggaacttat aatggaaact ttgtcattat ggtaacaaac
      721 gaagatttgg gtaatggaac gctttatacg acccaatcaa aatttagagt gtatgaaaac
      781 tatgaggaaa gctttgaaga ttatgccaaa ttattaacta agggaattag tggaaataaa
      841 gatttttatg caggagcatt aaaagctaat agtaaaacat atcgagaagc aactaaattc
      901 cttacaggac ggtatgcaac agatacacaa tactatttga agctaaatga attaattaaa
      961 acctacgatt taaccaacta tgataaagaa gttaaaggtc cacaaatgag taagactgga
     1021 taccaagtgc ctattcataa ttattcaata accagtttgt ttggaaatcg tggaaatgaa
     1081 tttcaccgag gacttgatat ggcagctaca cagggtgagc cgattcatgc agttaaagct
     1141 ggtaaggttg ttaaagcaga gtttcactac tcttggggaa attacgtggt gatacaacac
     1201 gaagatggat caaccgcgtt atatgctcac cagcaacaat acctagtaaa aactggagat
     1261 aatgttttgc aaggacaaat aattggctat gtaggaagta ctgggaatag tactggccca
     1321 caccttcatt tggaaatttg caaagatagt agtctttctc agagcatgtt agttgatccg
     1381 aaaaccttat tattcggaga ctaaaaaagg taatcaatct gacaaagtaa caagaaaatg
     1441 gttggattct tagacctttg atcaggatat tgagactgga attgatatct cgattacgat
     1501 gggtttgaca aataaaacaa gtccattatc gaatatcgag tgtattattg ccgtctttat
     1561 gctcatattt tgagcaggtt tcactagatg aaaacaattt atctatcttg aggatttcgt
     1621 gtacgtatcg ttcagtcttg tattgtaaat tagtcataaa atcagactaa gaaaaattgg
     1681 aaatattttt ttctgattta ttaaaagtta attgaaatta tatggtactt ctttcttggg
     1741 agggtaggtg cagtgaagga aagatatggt caccttactg ttgttgagga gattactcat
     1801 aatggatata aaaaatatcg ttgcctatgt gactgtggga atgaaacgat tgtatatcga
     1861 tcaaacttac tatcaggtag aaccaaaagt tgtggatgtg gaaattataa aaataggcat
     1921 aaatataaaa atttgacaaa taaaactttt ggtaagttag ttgcaatcaa gccaactaaa
     1981 ctgcgaagtg aaggaactgt tgtttgggaa tgtcaatgtc tgtgtgggag aaaaaagtta
     2041 gtatcggcaa agcgacttct aaaaggtgat gtaaaaagct gtggatgttt gaaatcaaaa
     2101 aagcatcaca ttgaaaataa aaaattcgga ttacttactg caaagtatcc tttggacaat
     2161 aactgttcat ggaaaacaaa atgggtttgt cattgtgagt gtggcagtat gtgtgagata
     2221 agttatagta acttattttt tggacatact caaagttgtg gatgtttaaa aaggcaagaa
     2281 acaaggacat gtgttgaagg gacagtaata gagtgtctaa cgtcaaaatt accgaaaaat
     2341 aatacaagtg gtgttaaggg agtttatcgt tctaaagaga aatagattgc gtatataact
     2401 ttaaaaaaaa agaggtacta tttaggatcg tatgattcta ttaagagtgc agctcaagct
     2461 agaaaagatg ctgaaaataa attgtttcaa cctttgattg acaaatatcg aggtaatcat
     2521 taattattgt ggataataat taacagtgat atataaacat ctataatata ttattcatac
     2581 tattgatatt tagtataatc ctatggaatt gaatatcata gcaatgtatt tgaaaagcaa
     2641 attggaaaac aaataggtgc aacaccataa atatgaaaaa attgggaaaa agtagaatgg
     2701 aaatttgctt acgtctcacg agatagctca cactattaat ctgaagaaca atgaagtcga
     2761 cttttagaaa ttgctagaag aaagtaaagc gaattatcac tagttactat agagtaagtt
     2821 caaataaagc atctccaact tacatggtta catgaggata tcaaatcgaa attactcaag
     2881 cttcctatgg ctagagtcgc gagaagttgt tgggcagtag acataggtta tattgttctt
     2941 agctacacga tcattagaaa ggaaatccta ttatgtatag ttatcaagaa gatgcaaaag
     3001 tccaaaaaaa tcgctggtgg attttgattg ctatatctat gtttaccttc atgtccacat
     3061 tggatggcag tattgtcaat attgcactac ctaccatctc agatgatatg ggcataccaa
     3121 tgaatcaagc ggaatgggta gtgtccgttt atctgatgat tgtctgttct tgtttgttgc
     3181 tgtttggtaa aattggcgat agccgtggga aaatcaaagt atttcggatt ggtacgctga
     3241 tctttgtctt gggatcgctg ttgtgtgggt tcaatcattc gctaggtatt ttattgttcg
     3301 cacgggtggt acaagccatt ggtgctagta tgactatggc tacaaatgcc ggaatcatca
     3361 cggaagtctt tcccatacat gaacgtggtc gggcattggg agcaataggt gcgtttgttt
     3421 cattaggctc gattgctgga ccaggtcttg gcggcttgat tttgtcccaa ttttcttggg
     3481 cgtacatttt ttggatcaat gtaccggtgg gaatcgtaac gatgttgatt gctgaaaaat
     3541 tcttaccaaa agatatcacc atgagcggcc atacaatcga tggagggggc tttgctgcca
     3601 ttgcggctac gattatcagc ttttttggag caatcttttt ggggcaagaa agtggctttt
     3661 tccatccagt cccccttgta ctgttcgtaa tttcagtgat tgcctttgtc agctttttaa
     3721 aagttgaaaa gaaagcagcc tctccattga tttctttcgg tatttttaag aacaagattt
     3781 ttaccatgag cttgattaca gccttgttga ttttttcttc caacttcttt gtcaatgtag
     3841 tgattccttt ttatctgcaa aatgctcgcg ggttgagtcc tagctatgct gggctgttga
     3901 tgatggtctt tcctttgatg atggtcgtcg gggcgccttt aagcggctac ctgaccgacc
     3961 gggttggccc ggagattttg gtattagtag gcttgggctt attgtcggta acccaggtca
     4021 tgtatatgtt tttgtcagtg gactctcctt tgtggctata cattgtcgct actgccatca
     4081 tgggtgccgg caatgccttg ttccagtccc ccaataatac gatggttatg agctcggtca
     4141 ctatcgaaaa cttgggactg gcaggctcga tgaattcctt tgcccgaaat ttggggatgg
     4201 tcagcgggat ttcgctttct acgacggtct tatatcaagc gatgtctgct aagatgggcc
     4261 agcaagtaac taactatatc gcaaatcggc cggatgtctt tttatatgga atgaagatta
     4321 cctttttagg cagctttgtt ttgtgttttg ttgccttttt cctgactatc tggcgtatca
     4381 aaaaacaagg aggtattcag aaaaatgtct gaaaaaacca ctgttgaaga atttttggag
     4441 gcctgcgtat gggtgagtgg gatttccatg gatgatggaa gatgaagtga acttctaaaa
     4501 gccgaaaaac tcaagtatta tcttcaaaat actagtgctc aatgagatgc ttgatttgat
     4561 cacaagaaga ctgcgaaagc cgctggcaac cgcaagctag gtatcccacc gatggactac
     4621 gttttagagg ctactggtta tgaatacgga gccaacgctc tcatctgcat aagaatacat
     4681 cacccagatt atgtaaagat tttcgatact tctgtcaaaa aatcattaag atagataaat
     4741 gatttccgtc tagtcaaatt ggttcagaat gtagacacac agatggtaag aagtcactca
     4801 caattcgaga atggacttgt cctatttgcc gtatacatca taatcgaaac gtcaacgata
     4861 gtatcaatat tttgaccaag ggctatgaat acaagcattg ccttagaaaa catcaagaac
     4921 cttaggaaca acggggatag cttggttaat aagagatacc gctgtttagg cttgcaagta
     4981 tactctttta tcaagaattc cgtggcttta gtcatgagag gcttaatact atgaaaattt
     5041 tctttagcat ttttgattta ggttcctttt ctaattataa taagatgacc actacagtaa
     5101 cctactgaaa tatataaaca gccactgtag taccattttc aaagtagtct atgagaggag
     5161 aatcttcatt cttatgaacg ctggaattgc ggatatgaat ttaataaaaa aaacccttaa
     5221 tgattttact tccaattcaa tatcaaaagg tactggtatc aatttaagca caataaaaaa
     5281 gctgaaaagt ggtgaaagat cagtggaaaa gctgaatctt ttagatgcta taaaaataac
     5341 ggaatttgct atgaaaaatg gaaaagctga gattgaaata tggagataat tatcgtctag
     5401 tgaattactt gccacttagc tgaatttaac gatccttggt gcttgaaggg ggagattctt
     5461 gggaatagag catacttgca agcctatgtc tttactaaca acgatatctc ttattaacca
     5521 agctatcccc gtagttctaa ggcttttact tttatctaag caataatcgt attcaaagga
     5581 aatttttgac ccttagaaag tctcatttta tcagacgtga gcttaggcat tgctgaattt
     5641 tgttaattcg gttttaggtt gtgctttgag cataatatga aagtattctt tatcacgatt
     5701 tcattcatct aaggtaatgt gatgtgattc tgaaattctt tcaaaggcag tttttgcata
     5761 agctgacatt acatcatcaa tgacatgttt acaatgtttc actactaaaa taagggggta
     5821 accaagaaga aaaactgaat gattatttgt atctaattcc atggtatatc aaccatttcc
     5881 tatatagact gattatgaca tggattaaaa taaaaaggca ctatgtctta attgtcggtt
     5941 ttcgagttac cgaatggcat tcatcaccca cctatagagg atgggcgact tctgcctgaa
     6001 ataagttaca ctacttataa cttctaataa aattagattg agtcattatt atttaacatc
     6061 aacgattttc cagtttccat ttttttgtaa tactaatttg tactgaaagt agctagtggt
     6121 ttttgataca ttatcaaggt attttatctt tgtactcact agaaaatttt ttccgttttg
     6181 tagataaact ggattctcta actccgaaaa ctttaagtca atattgattg gtcgcatcgc
     6241 accattttca acataatact ccaattcttt ttcagaagct gatggataaa gtttaaaaaa
     6301 tgtttctaaa aaatcggtga tttcttccgt tgttttggca tcaatttctg aatcgttttc
     6361 tacttgtttt gcttcatatt ttgccttgtc tggctttcca atcattgttg gcaaactagt
     6421 tactaaatga ttcctgtttt catcttcaaa gattgtcact cgatatgcag aagaaattga
     6481 ttgcgtcttt tttccctctg taatatgttg ctccactaaa aatgaaacga caaattcttc
     6541 cgagtgtttt tcaacatcta gaatttttac agattgaact tcggaactcg tgggaatatc
     6601 tgctcgaacc atatcttgga ttaaagcgag accgttttct gtaaggtact gttcgagatt
     6661 actcattctc ttttcaattg cttctttgtt atttttccat gaaaaatatt ctttcgcaaa
     6721 atcctctgta aaactttcaa cgccagaagt attcactacc ttctctttta taaccacttt
     6781 ttcatgagtt gtatgttgat caatggcggt gaaattttta taaacgccaa aagcaagact
     6841 acaagacaat aaaagccaaa gggcaagcac catttttcga tgcttaccca cggatacaac
     6901 tcgttctttt ttcttttttg ggctcttttc cttttgattc cgttcaattt ttatcttcat
     6961 tctttttcct cttttctctt atttgtatcg gccagctccg ataagatgct gccgccaata
     7021 gttttccgtt agattcgtcc accccactgg atctcctgca tgaaacatgc gattctcccc
     7081 tgcgtaaatg cccacatggg tcacataagt accggcatca taggttgaat gaaagaaaac
     7141 taaatcacct ggtttggcat cttttagatc aatatgagtc atggcatcat attgatcctg
     7201 agccacccga ggaagtttca ggtcaacttt ggcataacac cattgggtca agccactaca
     7261 atcaaacccc gtttctggac ttgagccacc aaacacatag gccgttcctt catacttaaa
     7321 tgcctcatca aaaatttctt ggactatttt gtcatcaaac tgagttgtag ttaggtattg
     7381 cttcactaat aaacagtaaa acatattgcc ataattatac cgccacccac cattttcctt
     7441 aatcgcgatt gggttggggt aatcgacttt ttgaccacct gatttttcct ttgaaaaaga
     7501 ttcagctaaa gcaaaactat gcttcttccc gtttttcccg acatgatcta gatacccgcc
     7561 accatagtta taggcctgaa taatactatc ttgatcacac cctagttctt tggaacgctt
     7621 aaccaaagac gcaaaataga gacagccttg tttgatggat gcctcggtac ttaatgaatt
     7681 aggtggtaat ccggctgact cagaactttg cataacgtct tccattgtgc caccagattc
     7741 cacttgaata atcgccaaaa gaattggaat atactctgtt atatccgatt ctttggcata
     7801 cttttccacc attgcacggt gtttcaatac ttcttctgag acactagttc cgccaggaga
     7861 agtgccagaa ctaccatttc cctcactgtc ttcaccaaac aacaaaccca cacacatcaa
     7921 aatacccata aaagagatgg tcagaaaagt taatgaaata acacccactt tcttcaagtt
     7981 cattttttct cacctgacct tgattttgga cgatggttca ttcttttttg tactttttga
     8041 ttggtctgct ttggcagagt ggacctctgt ttcacttgtt ggaaagaatt tttcttactt
     8101 gctgattggc ttttcccttt tgaaatgatt tcttgactag ttagttttcg tttgatttcg
     8161 ggattcagat ttgctctcac aatccgcttt ggtgcttctt ttttcagcat catatctttc
     8221 tgaggttgag ctgctttatc tattggaata ttcgcccttc tttttccctg acgatttgtt
     8281 gcttcactca gtatcttgcg tttttcgtcc attttcgcat ttcttcgttg tcgttgtttt
     8341 tctcgcaaat ccactcgatc cgctttttct tggaacagtc cgcgcttgaa ttcttctggt
     8401 gcttttttcg acttttcgat tcctttatgg agattgtatt tcaaattcgt tggcgtattc
     8461 cttacctgat cctttacttg tttagtctta tctttcacca aagcttgggt atccaaaact
     8521 ttgccgattt ttctcccagc caaattcatt cgggattgtt tcttattatt tgaaactgga
     8581 ttctccttag ttaagtcaga tagtatttcg tgatcgttcg gtaatcgtga attctttcgt
     8641 agagaagaac cggatggctt aaatttggat tttgattgtt cctttgccaa cttatttccg
     8701 actgctgcac caccaccaaa agccaaagct cgtcccacat ttcgattgag ttgccgcaat
     8761 tttcgattca ttaacatttg cggttttcgc atgacgcgtc ttcccattga ctggctatca
     8821 ttactctgga ggttgaacat actcataatg tcgcccaatt tgaaatagat accggcaaag
     8881 gtcacaattt gcagaaaagc aaccatgaaa aatggatagg ttgccgaaat attgaaaaac
     8941 atcgtggaga tgctaaatgc agtcgtaatc accaaagtaa cacccgctcg catcataatc
     9001 gtattgaata aacgaataat cgccttcttt cctaaacttt cataagtcgg caacatggac
     9061 aataagaaac taattggcag aaacatcgca aagataatga agagaatttg cgagaacaac
     9121 ataatccctg ttagaagaaa aacaaagaag gaaatgccaa tattgaacaa taccaaaaag
     9181 accacggtcc ctaaacggtt catggtcttg gtaatactga gattcgcatt gtcattgtct
     9241 tcaatctctg ccttgaccgc ttcttcccga tctttgcctt tgttttcatc tggactcaca
     9301 gatagaatct tgttgacccg gtcttcacca atttcttcta tgttggaatc atcaaattgc
     9361 agtagtaacc aaggttgttg gacttgaatc gaaaacaaac tatcccgaat caaatccaca
     9421 ctatcttttc cttggctttc tgaattgggt acaacgatct cggttccaag ggtaagggcc
     9481 gcttcactaa catctgagct aaagtcattg atttttgtga tataggtagg cgcataagca
     9541 atgaacgaac ccgatagtag aaaaatcatc acaaaattaa tcacggctcg aactgctttc
     9601 gtggtttctc gttttaataa tccggtatac gccacataca cccccagagc taaaatcatc
     9661 aataatagaa acccaaaata aaatccggag gtctggagtc cattttcagt aataccagcc
     9721 aatgtttgga tattctttcc aatactttca gccgtatccg aaatgaaatc taatttgtaa
     9781 gcctcttgaa ccacataacc cgtggcgttc gacaaataca aactcaccgt ccaaatgaaa
     9841 ttagtaatgg cataaagacc atacatcacg cttttcccta agccatcacc ccaattccaa
     9901 ggcaaccaat cccaagagct atccacaaag aaatctagtt gatagttgtt cagtgaatat
     9961 ttggaatata gatttcctgc ttcaactgta tcatcaacta gccctgtcgc ctcggctacc
    10021 gtaccaacta aactcaatag aactatcaga gtgagtatgc taataaaaac gattcccaca
    10081 atccgaaaaa gtttcttttt catcccctca ctccattctc tcttgaacag gtggacgggt
    10141 atcaaaggca tggaagagtt cttcaaagac tggatgtacc tgaaccacgc ctacccgacc
    10201 atacaaatcc tgcatcaagc attggccatt ttccaaatct cgtaggcgtt tttgattgct
    10261 ttcatcttct cgatccaagc caaagaattc caaggttttc ttgatttcat gaatatcggt
    10321 actgcgaaac gcaaacttca acccgatatt attcttcaag cgttcatcca gtaaatcatc
    10381 actattttgc gtgacaaagt aaaccccggc attcatagcc cgcccagcac gaacgagctt
    10441 attcgataat gttttccctt gcgctacttg aagaaaactc cacgcctcat ctaagtctac
    10501 aattttgaag atttctcgat tactatggat aaagtccaag gcaaaagtgg aaatgacgat
    10561 tagcatggaa acactaagca attccatggt ggtatattct tcaaaagtgg tttctgcatc
    10621 tggtaaaacc aaatccgcta cttgaatgat atttaactgc ctatctaaac taatcgactg
    10681 ggtaatcgaa ccatctgaga aaagcaaatg ggcaaaatca taatcggtaa aggactcgat
    10741 gtgatccgcg atattttccg caactggtga gccttctttt cgaagctcat taattacttg
    10801 taataatccc cgttgattgc tttgggtaac cgtccgaatg gccttacgca acactggaaa
    10861 cttctcccca tctcgactag aaattcctgt taaaaaggtc agaatatcaa ttgctagact
    10921 ttcagaatcc ttctttttct ccataatcac ataaggatca agtaatcctt gattttttgg
    10981 cttactagtt aagttgagaa tattgatttc atccgcaatt tctggcaagg tttctttcca
    11041 gccaccccgt tccccttttg gatcgacaat cacagcttgt ccaccaaaaa ggacggcgta
    11101 ataaactaag agattgttac taaaggactt tccgccaccg agtgaaccga ggaatgccgc
    11161 tgctagcgca ttggtaactg aatccttgac cccttgtgag gccaatgccg gtttcaggta
    11221 aacattgcgc cccgtatcta cgttataacc aaagtaaatg ccttccaact cccctaacat
    11281 ctgagtggcg ccaaaaccaa gtccggcgag aaaatcagac gtaacatact ggatataatc
    11341 attcatgtat cgcttactag aaggtaagaa ttcttcatgt agccccatca tatcgccaaa
    11401 aggccggact agttttacat tggtatcatc ataaaaatct agcacttcat cacaccggcg
    11461 ctttagttca tctactgatt cggcacttac tcgaaccaca tagctcagtt tgtacatgga
    11521 ttctttggac tgatccaagg tagtttccaa ttcatctacg gaatccaatg catctaagac
    11581 attggaattt gtttcattgt cagattgata ggcatggttg tccaagtctt ttaattcctt
    11641 tttcttattg cgaacggtag caagtgcctt tttgttcgtc acaatctcca cattcatact
    11701 agtgtcaatt gggaatgtaa attgttgttg ctggtaatag aaaagttctg aggagggaaa
    11761 ctccatttcc cccacaattg tattaattgt taagtacgct acatatgatt catgattctc
    11821 atgttccatg cataagtacc ggggcttttc ttcaatcaaa caacgactgg gacgtaataa
    11881 gtcgtaacgt ttcaccaaag tttccgactt cagctttttc tttggtagct gaaattcata
    11941 ttcttcaaat ggtgtaccct tttcgccata gatatgttca atcaaataca tcagatcact
    12001 cggtgtgact cgtctgactt taaatcttcg agccaattta ctttccatta gtttttcaag
    12061 cttggtatat cggcgaattt cttcattgga taaggaaaca aaatcgccca tcaaatgatg
    12121 attcactcca tagacaaatt cttgaaagcc ggagaagaaa gatttcttga gattctttag
    12181 attgacctct tcatctgtcg caatcagttt gaagccaata aaaaagcgat aatcaatctg
    12241 actatcgcca atcatggata ctaatgcctc tgtctgtaaa tcaattcgtt gtttcgctac
    12301 ttctgttaat cgcccggtaa tttccttttt tgatcgttct tgcgtggcac gaatactact
    12361 ttctgtggca atttgtaagg catgaatctt gccttcacga ttttgcgcaa tcaattgacg
    12421 aaagttatcg tggacttgat atttctgttc aggtgacaaa aaggagtaat tgtaaggtat
    12481 taactcatag taggcaaaac attctccctc ttgattaaag acaagattat tttcaatata
    12541 cttaatcggg tacatagttc acactcctta ccagagtaat gggttcgtcc catcgtttct
    12601 tctgttcttt gactttcttt cctgcgaaag tgaccttaaa tcgtaagtga tagctaataa
    12661 aagatcgtaa aaatcccaat ggtttcttac cgtcaaacgt tttttgcgac atgaaccaag
    12721 taactgccac cggtatacct aagtatttta aaaaggcccc ctctatcata gatagtggag
    12781 gcacatgggc aaatacgatg acaaacagca aggataaaac aaaccaagcc atctggttaa
    12841 aagtcacagg aaaaggcagt tgaaagtcat taattgcata aatgaccttt tcaactgccc
    12901 aaatactggt gtagcttttt attttcttca tcaaatcatt cctttaaaaa gaaggatagt
    12961 ttagaaatca actacccttc aataattttg ttaatatgga tactcaaaaa cacctcgact
    13021 cgttaccaaa taattgcctc caatctccaa gtctctacca cacgcatcaa aatcaatata
    13081 cgtcttcaag tgttctggta cttctccaaa atcaccagct tctacaatca agaaatatgc
    13141 tacatcactc atatcatcgc aatctggata atagataata tcatccacat gctctaccat
    13201 ctcctcaaaa gaattgaaga aagcatgttg aatctcagaa gcaacttcac caactggcgt
    13261 tccttcaagt tcctccgcta agccacacaa ccgattaatt tcctcaattg gcgtatactc
    13321 atcaatctca aagggaagtt cataatcatg aattgcatat tcctcgtatt cgtcatttaa
    13381 cccaattcgt tctttcactt catcaaaatc gacaggtggg gtaaaccacg cccccaccaa
    13441 ttcaccttcg ttatatttcc cgaggttcgc aatataaaca cgcatctgtt ccatcgtgct
    13501 tcccccttac gcaccgacaa ttcggttaaa caaactgagt aagacatcct tcaccccaga
    13561 agcattaaag accaatccga ccgcaatcaa agcaatcacc aaaaagccaa tcagtttgga
    13621 aaactcccgc ttaaatccta aaaacaaacc aatgaccaca attgccagta agactaagga
    13681 ttg
//
LOCUS       DARBEC010000074        13429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.076, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000074 DARBEC010000000
VERSION     DARBEC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.076"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..914)
                     /locus_tag="SCL80_002101"
     CDS             complement(<1..914)
                     /locus_tag="SCL80_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEG1107952.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="SCL80_002102"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="SCL80_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEG1107953.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="SCL80_002103"
     CDS             2006..2176
                     /locus_tag="SCL80_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107954.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEETALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="SCL80_002104"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="SCL80_002104"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEG1107955.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="SCL80_002105"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="SCL80_002105"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEG1107956.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDTDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="SCL80_002106"
     CDS             4440..5321
                     /locus_tag="SCL80_002106"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEG1107957.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="SCL80_002107"
     CDS             5318..6034
                     /locus_tag="SCL80_002107"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEG1107958.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="SCL80_002108"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="SCL80_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEG1107959.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="SCL80_002109"
     CDS             6751..7752
                     /locus_tag="SCL80_002109"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEG1107960.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="SCL80_002110"
     CDS             7749..9110
                     /locus_tag="SCL80_002110"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEG1107961.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="SCL80_002111"
     CDS             9070..10221
                     /locus_tag="SCL80_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEG1107962.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSGAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="SCL80_002112"
     CDS             10196..11365
                     /locus_tag="SCL80_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1107963.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="SCL80_002113"
     CDS             11540..12919
                     /locus_tag="SCL80_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1107964.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13429
                     /locus_tag="SCL80_002114"
                     /pseudo
     CDS             13200..13429
                     /locus_tag="SCL80_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         4482 a         2340 c         2926 g         3681 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaagaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat acagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcatacagt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcgg ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgca atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagt
//
LOCUS       DARBEC010000075        13414 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.077, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000075 DARBEC010000000
VERSION     DARBEC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13414
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.077"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            136..309
                     /locus_tag="SCL80_002115"
     CDS             136..309
                     /locus_tag="SCL80_002115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107965.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="SCL80_002116"
     CDS             949..1422
                     /locus_tag="SCL80_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEG1107966.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="SCL80_002117"
     CDS             1431..1658
                     /locus_tag="SCL80_002117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107967.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="SCL80_002118"
     CDS             2294..2665
                     /locus_tag="SCL80_002118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107968.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="SCL80_002119"
     CDS             complement(2921..3163)
                     /locus_tag="SCL80_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1107969.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="SCL80_002120"
     CDS             complement(3195..4097)
                     /locus_tag="SCL80_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1107970.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="SCL80_002121"
     CDS             complement(4110..4298)
                     /locus_tag="SCL80_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1107971.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="SCL80_002122"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="SCL80_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1107972.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="SCL80_002123"
     CDS             complement(4913..5806)
                     /locus_tag="SCL80_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1107973.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKVAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6807)
                     /locus_tag="SCL80_002124"
     CDS             complement(5884..6807)
                     /locus_tag="SCL80_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1107974.1"
                     /translation="MDEEKNVIETDEKKYNWLVLDSAKPEEIEQAIEEFELPDDIFVG
                     TEYPEEVSRMERLYETKLEHPFSLVVINLDQSDNRIEKKLTPISFVLSDNLLITCIDR
                     KTEFVDRLLTKHGNHLNSFEKIITYTLLDIYTHYVKELREMKKRIDALDKAARKTTEN
                     EELYKQADLERDIVYIDHTLRDQRETMDQLWNSETFIKRLDDEQLLYDVHLRQRQTEK
                     MIEIYRDLLESIGDLFNGMMDNNLNHIMKYLDSAALIISVPALFAGIWGMNTGGLPGK
                     SSALGFILVVFGSLIAAVVLGYHLYRKDYTN"
     gene            complement(6856..7206)
                     /locus_tag="SCL80_002125"
     CDS             complement(6856..7206)
                     /locus_tag="SCL80_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1107975.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7239..8141)
                     /locus_tag="SCL80_002126"
     CDS             complement(7239..8141)
                     /locus_tag="SCL80_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1107976.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8134..8991)
                     /locus_tag="SCL80_002127"
     CDS             complement(8134..8991)
                     /locus_tag="SCL80_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEG1107977.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9325..10134
                     /locus_tag="SCL80_002128"
     CDS             9325..10134
                     /locus_tag="SCL80_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1107978.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10174..10671
                     /locus_tag="SCL80_002129"
     CDS             10174..10671
                     /locus_tag="SCL80_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1107979.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11056..11129)
                     /locus_tag="SCL80_002130"
     tRNA            complement(11056..11129)
                     /locus_tag="SCL80_002130"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11093..11095),aa:Ile,seq:cat)
     gene            11316..11639
                     /locus_tag="SCL80_002131"
     CDS             11316..11639
                     /locus_tag="SCL80_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107980.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11803..12057
                     /locus_tag="SCL80_002132"
     CDS             11803..12057
                     /locus_tag="SCL80_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107981.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12127..12372
                     /locus_tag="SCL80_002133"
     CDS             12127..12372
                     /locus_tag="SCL80_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1107982.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12448..12813
                     /locus_tag="SCL80_002134"
     CDS             12448..12813
                     /locus_tag="SCL80_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107983.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
BASE COUNT         3910 a         2477 c         2244 g         4783 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca actttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt tttcatctgt
     6781 ttctattaca ttcttttctt catccacaaa gtagtatctc aatgtatacc tcctttttgg
     6841 actttctcga aactattagc tgaagaagca gttctttaat ttgatattat aatcgagtac
     6901 tgcgatttca ttttctaatg aaaagctgtc aagctgtttg ttgaagtcca tgttcaatcg
     6961 tttaccgaaa taattttgct ctaatttaga tgtatagatg tccaataatg gatgctcgat
     7021 tttagtcgca tattctccaa attccgaaac aaaaggaatg atttgaaaaa agctgccttt
     7081 tgcaaaagca cctttgtccc ttagaaatgg atcggaatca ttgataagcg cgagtgcgat
     7141 ttttgtattt tccccaacct tttcaattaa cgtctctact gcatttttag acattgtaat
     7201 ttccattcct actccccctc ttcagttgtt gtcttctatt atttaacaac tacatctgtt
     7261 tttggttgaa cttttgcata ttttggatat ttagggaatt tagctacaca acggaaagcc
     7321 gtcatccctg catatttttc gtaatatttc ttcgttggcc cttcaaaatc cttgctccac
     7381 ggtttcacat gtcctgaaaa atggataatg taaggattgt tgcgagtttc ttcgtattca
     7441 cgttcacctg ctgctgttgg atgttttttt gcttttgcca tgatatagcc ttgtgcattc
     7501 caacgcggat gaagtggtaa ccaacggtca tgtaagatag catttaatgc atcttggtca
     7561 tggaatcgta atttttctgg attatgttcg atgaaatcta atactttttg cgtaatattt
     7621 tcatctaacc attttttcac attgatcagc atcaaacctg agttgaaata acgcattgat
     7681 ttagctgaaa ttttcatttt ttccagtcgt tgatggaacc cagcgtcttc aacagctgct
     7741 acgattgaat caccgaagtc aagacgccat aatttagaga tatcttgtaa agcgatcatg
     7801 tcgcaatcca tatataagat acgttcaact tctgtgccgc ggaacaattc cggaatcgca
     7861 atacgataat aagctgtttc tggaatacga tcgctgatta ccacatttgt aaagaattct
     7921 ttattgattt tcaagaattc cacatctgag ttcattcgtg cgtttttcac tgagaagcgt
     7981 aaaagctgtt tactttcaaa atcgatatca tcgtcaatca catagaaccg aacaaattta
     8041 gacgggttgc aattatctaa tactgagaca aataatgcag ctaaatgtgg tacgaatttt
     8101 gtgttacaac ttgcgactac tgctatttct tttttattca ttgatctcgc ctactttcgt
     8161 tagagtactg tgtgctaatt tttttaagta taaattggta tacggatgat ctttcaacgt
     8221 attccaaggt ttatcatgtc ctgtaaagtg aacgatcgaa ggtttttcat ttccttcttt
     8281 atactgacgt tcatattttt catttggtgc tggatgacgt tcaaaaatca gcgaagtctg
     8341 catgttccat tttggatgaa gttgttccca atcttcataa agtacagcat tcaaagcatc
     8401 ttggtcgtga tagatgattc gatcgccatt ttcactgagg taatggatag ttttttctgt
     8461 aatttctttt ttattccatt gatcaatgtc aatcaccatc actcctgaat tgaagtacaa
     8521 gtcatcggaa tcgattccta atctttccaa agctttcact tggcctggat cgataatagc
     8581 gccgattgtt tttccattta acggctcatc atatagtttt acgatatcgt ctagtactaa
     8641 cacatctgaa tctaagtaaa ggactttctt ataatcctct ttagctaata agtttggtaa
     8701 agaaattctt aagtaagcgg tagttgtgat atggtcactg actaggaagt cttcgtagat
     8761 atctttttcc actgtcagga attggatact tgcattactt gaatatttat ttaccgtttc
     8821 ttccaatcct tctttactat attctgatag accatcgtca atcacataaa attttatgcg
     8881 tcttgtttta ttgcaatttt ctaatgcagt tgcgatcatc acgctcaagt aaggagcata
     8941 attttcgtct gaagcagtta ccacaggaac tacgccgtat ctcttctcca tcaaaatcac
     9001 tcctttttct aatctttttc taacctgttc tacatctaga atattcctca tttggtttta
     9061 aagcaaaata tatgctttgt tttcattgaa tattttaaaa tttttttatc acaaaacgca
     9121 tcggtaagcg ctaactattc gtttttcatg gctttttatt caaaggtatt gaccaaaaac
     9181 tactatatat agccaccttt ttcttaagaa tgttttttga aaaaattcat aatgacaaag
     9241 caagttagtt tcattttttt aaattctcat ttaatcttaa ggtaacagga aaatagatat
     9301 gtaaaaagaa agtaggaaga taaaatgaaa ctagcagcga ttgatttaga cggtaccttg
     9361 cttgatcgaa atggaaagat accagaagaa aatatccaag cacttcggaa atttgatgaa
     9421 aatggcggaa tcgtaaccgt cgctaccgga aggaacagca tttctgctaa agatattttt
     9481 tccgaattag gaatcggtgg ctatttgatt tcttccaatg gttcactgat tgctgaaatg
     9541 aaggatggaa aaatggcgaa agttctcaga cgttcaaaaa tagatagcga tacactgaga
     9601 aaagccttct tctatgctca agaagcgaaa atatccatca ttgctagccg agaaacccaa
     9661 gatgatcagc tgacattcga tgaatcaaat ctagtcaaag atgaccctta ttatgaacat
     9721 ttcaatttac agaaccattc ttttgatcaa attttaaata tgttggaaga tccttctctt
     9781 agctatctta aattagcatt aacagataag agtgaagaaa aactacagaa gctacaaaga
     9841 gatctgcaag cagatggtat ccaatcagta ttctctgatc cgcatttctt agaaatcacg
     9901 cctacaggta tcactaaagc tcattcactt gtttttctgg cggattacct tggaatcgaa
     9961 tcgagtgaca tcatggcttt tggcgatcaa gaaaacgaca ttgctatgtt gaattacagc
    10021 gggttgagta ttgcgatggg gaatgcacag cgacaagtaa aagaaattgc aaaccaagta
    10081 acactgacga atgaagaagc tggtgttgct tatattttaa aaaacttcta ttaaaaagaa
    10141 taaaatgacg atatgaatgt aggaggaaag aaaatgttag ttattattga tatgcaaaat
    10201 catattcttg atccaaacag cgaattttat attccagagt cagatcaatt ggcagaacgt
    10261 attgctcaaa gattagctaa agcacgccaa aataatgaat atattctctt cacacgggat
    10321 ataccagttg aactgaagga caaagaagaa gaacgagaag atttgcaatt gatccctctt
    10381 ctttctccac aagaaaatga acgagtgatt aaaaagtatt actttacgat tccacctgaa
    10441 actttagctg aaatccagga aactttattt gaaagcaaag aagaacaaaa agagatcgaa
    10501 gtagtcggga ttgaaacaaa cttgtgtgta ctctctaatc tgataggcct gcagagtgct
    10561 tttcctgaag cagacttttt tgttgatcca atgttagtct ccagtagaaa acacggagaa
    10621 tccgctttag agctgttgaa agatttcaac gtctctatcg tcgaatcata aaaatatcta
    10681 tcttcattta cgatatttaa cacacaataa aaaaagcacg cagaactgtt ttagtcctac
    10741 gtgctttttc tatcaaagta gattggcttt tttatgacac agagtgaaac ggcaggctca
    10801 cgacctcttt cttaaatggt gttcaatcag ctgaccctcg gtattagctt aacaaacggc
    10861 aaaatatgag gaactatttt tccgtttgtt aagctaatac tcaggtcaaa acgctgattt
    10921 cacaacctct tccttaaacg gtattcaaaa gcttgcatct gcagtaataa gaacagccaa
    10981 gtaaaaacat aagaagctgt ttttacttgg ctgttcttat tacttgatgc agggcagctt
    11041 tttcacaacc tcttatgtac cttgtaggac tcgaacctac gaccggacgg ttatgagccg
    11101 tctgctctaa ccaactgagc taaaggtaca tcataaataa aatttatttt tgttactttt
    11161 caactatagc acgaatgaca aactctcaca agtttcaaaa tataagtaaa ataatattgt
    11221 tattcttatt tcttttactt gtgtcataac tattttgtta ctttaatttt tatttgctta
    11281 ttttagaaaa aatgttattc ttttatttat tctcaatgtt aggagtgatt attttgccat
    11341 ttattgcgat tttattcgat ttagttgcgg cagcagctta ttttctccaa ttaaattatc
    11401 aaacctcagg aattctgttt attggaatga tttttcaagg tgtgattacc cttttgcttc
    11461 ttgtcatgat gctcagctat aaaggaaaaa gatatgcccg tattcaaaca aaagtatttg
    11521 ttaaatatgt ttccattcgc tatgggatca tcattctttc ctttttagtg aatgcgatcg
    11581 tcctcttttt atatgtattg aattacttag atatcaatcc attgattttt tctcgataga
    11641 taagacacat tcagcggaac agttcctgct tatcagcaga aaactgttct gctttttcat
    11701 ttttcattga tctattcttc ttttattttc ctagcatttc atttgtgatg tatgcaataa
    11761 catgttatct tttattatat gaatcgttga ggagtccgtt acatgattac gttggtgaga
    11821 gttttgtttt gggtgcctgc ggtcgcactg gtcgcaagta ttgtttatct tatgaattgg
    11881 aacaaagaac ggttttattt agctattttg actcttccgg ctatctactt tatgtggaaa
    11941 gtttttaatt acaactactt tgaacccgat agtgttttta tagaagaatt atcaggactg
    12001 gttttgtctt tattgatcgt cattctttat ttgatacgtt taaataaaaa acattgagta
    12061 ctattgattg catgtgtagc tttctatagt cattataact attctactat aatgaaaggt
    12121 gattaaatga gtatcgaaga agctatttta ctggatactt tacgttgtcg tctgaatttc
    12181 ttaagaactg gccgatcttt atctcaagta gctgtttcta gcgcgttaag tattccttta
    12241 ttcaaatatc gtaagtacga agaaggagaa gaaattccaa gcatcattga attaatctat
    12301 atcgctgact tttacgatat ttccgttgat tacctaattg gtcgctcaga aacagcaagc
    12361 aaactgaagt gattttttcc agccttggtg ctggtttttt catttaacgt ctaaggttaa
    12421 caatctatat ataaaggaga catttctatg cgattatggc atgaaaaatt gatttcaaag
    12481 cttcctcgtc aacagctttt aggacaacat cgggaatgct gtgcattaag aggaaatggc
    12541 tggggaaaga aacatgctac tgtggattat gtttttcact attctcctta tagattattc
    12601 caatatcatc agcttgtcat ggatgaaatg gaaaaacgtg gttacagccc agcaccagag
    12661 tggaaaaatc ctatgtatcg tggcaaatca tgcgaagcat tttttgattt acctactgta
    12721 gagcgcaacg tgccgattta tccggaacac gatgaagttt atctagcaga atgtttagca
    12781 aatttggagc aaaaaggaat ttatcttacc taatataact agaaaagcct tctatctcct
    12841 cttacttcat taaaaagctt taaccatctt agatcaagct aaaatgtcgt aagttttagg
    12901 gttatcttca tcctcacgtg caaaactaaa agcgtactct agttttggtt catcgccaac
    12961 ttccctagac ataaatgaac atctcctgac gtggtttaaa agtaccgcat cttcgacgtc
    13021 tgtatacgat gcacagcaca gattgcttct tttgatcttt cttttgattt ttctgttgct
    13081 tcttctacac cttcttcgat tactacatcg atgcttacaa ttgcttctgc ctaatattcc
    13141 catgcatcgt cgatttaagc ctctgtagtc actgtgatcg tttccacatt ttaactcctc
    13201 tgtcgtggct tcagttgatt ttgtgatcag ttcagttgct aagccagagt cagaacaacc
    13261 tgccattgct agcgttaata ttgatagtaa aaatagtttt tttatcataa ggttcccccc
    13321 ctgaaaatag actaaaataa taacgctttc tttttttgtt ttcagacgaa aattaataat
    13381 ttctaaatat tctttaaaaa agcttataac tatt
//
LOCUS       DARBEC010000076        13399 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.078, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000076 DARBEC010000000
VERSION     DARBEC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13399
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.078"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(15..707)
                     /locus_tag="SCL80_002135"
     CDS             complement(15..707)
                     /locus_tag="SCL80_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1107984.1"
                     /translation="MQIELQHTHDKETFEKNYKVYFELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1144..1713)
                     /locus_tag="SCL80_002136"
     CDS             complement(1144..1713)
                     /locus_tag="SCL80_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1107985.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(1787..2110)
                     /locus_tag="SCL80_002137"
     CDS             complement(1787..2110)
                     /locus_tag="SCL80_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107986.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2264..3076)
                     /locus_tag="SCL80_002138"
     CDS             complement(2264..3076)
                     /locus_tag="SCL80_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1107987.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            3430..4278
                     /locus_tag="SCL80_002139"
     CDS             3430..4278
                     /locus_tag="SCL80_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEG1107988.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            4423..5364
                     /locus_tag="SCL80_002140"
     CDS             4423..5364
                     /locus_tag="SCL80_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEG1107989.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGLPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            5354..7525
                     /locus_tag="SCL80_002141"
                     /pseudo
     CDS             5354..7525
                     /locus_tag="SCL80_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCL80_002142"
     CDS             complement(7637..9808)
                     /gene="copB"
                     /locus_tag="SCL80_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEG1107990.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(9828..12014)
                     /locus_tag="SCL80_002143"
     CDS             complement(9828..12014)
                     /locus_tag="SCL80_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEG1107991.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCL80_002144"
     CDS             complement(12014..12223)
                     /gene="copZ"
                     /locus_tag="SCL80_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEG1107992.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12236..12676)
                     /locus_tag="SCL80_002145"
     CDS             complement(12236..12676)
                     /locus_tag="SCL80_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEG1107993.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(12750..13289)
                     /locus_tag="SCL80_002146"
     CDS             complement(12750..13289)
                     /locus_tag="SCL80_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1107994.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3765 a         2807 c         2381 g         4446 t
ORIGIN      
        1 gaggggggga ttttttatcg ttgtggaata tttaaatacc aacgcttgtc atgaaaatct
       61 tgtgctccac ccttagtatt tccttctgga tcgtttgtcg cacgcatcat gacgtatact
      121 ttcttattag ggaaatcacg catattgaaa gatacatgat agccaacatt tcctaaagta
      181 ttataagctt gatttacgtc tggtctataa atcccatcag ctcttactct agctagttct
      241 tttccagtat tgtagtccat aatgaaaata tactcgtatt tataattagc aatgtgccat
      301 ccagctacat gcaagtttgc gttttcgatt tctccgaact gatcaatgtg ggcgtaattt
      361 gttccatctg tcagcgtaga atttgcagca cctgctcgag ttggatcaat tactggttta
      421 tcctccgaag tagttggatt atcatccgta aaaccatgag ccaaatcata ggctagtttt
      481 tctttactta cacccatttc agaaagataa ccgtaaggat ctgtatgatc gccccaaata
      541 ttttgtgtta cccataaatg cgatttgatt cctggttggt tataaggagt gtctaatgtt
      601 aatggaatac catatttcat tgctgaatct cttgccaatt caaagtatac tttatagttc
      661 ttttcaaaag tttctttatc atgtgtatgt tgtaactcga tttgcacagg acttttggct
      721 cacgaggttg tcctactcta catcttgctg atagatatgc tgaaaggaaa acaccaacat
      781 tatgttttgc tttttcagct agaacgagac aagcaggaat acctttagaa gatatcaaag
      841 gttttttagg tcataaagat gtttccacta cacaagtcta tgcccatatt tctcccgaag
      901 tgaaaaaaag atcaatgaat caactagaaa actatataga agagcaaata aaaaagcact
      961 caaattgagt gcttttttaa gctcactatc actaaaacta tcactaattt tatttataag
     1021 cgaacatgtt tctatcactg attttttaaa tctagcgaaa aacccttatc ttaaaggcgt
     1081 tcgttcaatt ctttggcaag gtcttcaaag cctggcttgc ctagaagtgc gaacatggtt
     1141 ttcttatagc tcatgtagaa aaattttctt gatgatgatt ttatccatca attgagattg
     1201 actgttttgc tctcctatga tttcagctat gattgtccga ttttgcggtt ttcttcttaa
     1261 ctggtagttg attttcccag tttccatcaa ctcgctaagc atatccacga ctccgccaaa
     1321 tccggtcttt ttcgtaataa aacctagtgc taccattcgt tcatctagat tttttgctgt
     1381 aatcgtcaca ccagtaatga gcgagtgctg taatgtttgg gccagctctt ccatcccttc
     1441 agcattggat acgacggaag ctgttttgtt aataggttct tcgtttattt cttttggttc
     1501 tttcctctcc actttcactg tttgacgaag gacatctagt tcttgcaaga gcgtttcgta
     1561 ttgttcttgg gtcagcatca cgccagctac tttttcacga ttaaatatat atacgccagt
     1621 tcccgcgtct cgcgcttgag caaatacatc catcggcgat cgtttcactt ctgtgatcga
     1681 agatgtggga acttctaatt tctttaattc caatggattt cctcctttac taatcgcttt
     1741 ttattatagc acagactgac ttgttttgat cttttttcca aaggttttat cggtttttgc
     1801 ctaaccagta attgatatcg gtaagaagtc tttcgatacc aaatgaagga tggtacagat
     1861 acgttttttg atcttcgtta tggtctagtg gaaaaaaatg atcgatgatc actagatctg
     1921 cataccgagg cggcttattt cgagtacata catgtataga gatccgtttt cgatgtctta
     1981 gcgttttgtt cagtacccca gcaagtcttc ttgcttcttg gtgttcatag cgaaaaaaaa
     2041 agacgatttt cacttcagga gggtggacat ctaaataccc tcgttcctgg agtgttttca
     2101 gtaaaagcat cccccactct actgcggggt gtgcaagtgc ttgttggatc gctagccgaa
     2161 attgatatag tttatctttg gaattgggaa atttttctaa tgtttcctga taataacgaa
     2221 cttcttctat cagccggtca tggataccaa ttacgtaacg aagtcattct tggtaaagta
     2281 cttttgtcag ctttgatttg ctttcgctct caaattcgct attcagccaa aacagcgaat
     2341 caagcttttc agtcacaatc gtcaatttcc gatagtcttg tgtctcttct atccgtttcc
     2401 accgcttttc agtaatctgg ttacagctca agcgcaaaat tgctagataa tttttttctt
     2461 ttattggcaa aatctgctcc tctctaaata gcaattcagg aaacaattca aagaatagtg
     2521 ttcgactgtc tcctaaaatc tcgatttctg ccggatcctt tctttgcttc aataattgga
     2581 tgaacatctc agaaaaggac tgctctccat catctgcaaa aaaatgcgga aaatctttca
     2641 aaaagcaaaa cagcagatat cttacttttc tttctgaacc aaagatctgt gcttttttat
     2701 aagaaaaaga aaggtggtac tcgccaaacc actttctcaa tcgctcttgc gtagaacgat
     2761 cgtacttctg ctcgtaaggt tcattcaata gcagattttt cagtaaacaa aacgggggtg
     2821 attggatcag aaaaacaaac aagagataag gttcgctata tccttgatca taaactaact
     2881 gaacggtttt cttatgaaga atcagctgaa tgggacagtc gctttccgcc aaacgtttca
     2941 gatgccgatc cactgtttta acatcgattt tcagttcttc agctaaatca gcttttcgcc
     3001 agatcaaatc tttcattaga ttgattagtg aaaataaatt ctgtgcattt tttgataata
     3061 gtttttgcca tctcatcgat catttctcac ctgatttcta aacatattgt ttgatcaaaa
     3121 tgggttcact cctaaaaaga taaaaaactc cctttctatt tatttaaata cgcaaatctt
     3181 cctcattatt ttttatttta ctcaatttaa tcgtattctc atttcaaaac gaatgtactt
     3241 taaaaaaaca taataagatc actttgcttg tatgatgtat tcccttccat tacaatgaaa
     3301 taaatagcga ttttatgaga aggtgatgtg aaaatggaag aaaaaataga agaatccaaa
     3361 caaaaaatcg atcagttgat ataagaatta gaaaaagcgg tcatcagcaa acgttcaact
     3421 atccaattga tgatcgtggc cctattagca ggtggtcatg tcttattcga agatattccc
     3481 ggtgtaggta aaacgttact ggtcaaagca ctagcaaaag ctgtacaggg aaccttcagc
     3541 agagtccaat gtactcctga tctgctgcct agtgatattc ttggtttttc cgtatacaat
     3601 tcacaatcaa aagagtttga atttcgtccg ggaccgatct ttaccacgat cttgttagca
     3661 gatgagatca atcgtacgac tcctcgtact cagtcagcac ttttagaagc aatggccgag
     3721 aatcatgcaa cgatcgacaa caatacatac cctctagaca atcatttttt tgtcttagct
     3781 acgcaaaatc caatcgagta cgaaggaaca tatcctttgc cggaagcaca gctcgatcgt
     3841 tttttattcc gactacaaat cggttatcct tcttttgatg acgaactttt gctacttttg
     3901 gataaattcg aaaaagtgtt ggataactta aatgaagtat tgaacagtta tgaaatagaa
     3961 gaaataaaaa caaatgtggc gaatgtcttt gtcagtcccg aagtggcatc ttatgccttg
     4021 cagttagtgt cagcaacaag aagtcatgct gccatccagc ttggcatcag tccacgagga
     4081 agcctttcct ttattcaggc agcaaaagct tatgctttaa tccatgggag aaattacgtc
     4141 acgcccaaag atttgcagga tttagttcct tacgtgtttt cccatcgcct gattttttac
     4201 gatcgttcat tgaaggaaga cgaaaagaaa caaatcattc atactcttgt tgcacaaatt
     4261 cctattcctg tgaggtagat tgaggtagac aatggaaaaa atcaaagatt atttcattaa
     4321 ttttgtgtta tttctggtat atttcatcat tgtgcttttt gctcttgtat tcaacaattc
     4381 tttaggctgg ttcttgttgg ccttttttac tctttatgta tcatggagct tgatttcatt
     4441 aagcagcttt ctaaagaaac tttcggttac ttgtacattg cagccactcg tttatcgcaa
     4501 aaaaagtatg caggttattt tttccatagc aaaaaagaga actttttctt tacctctccc
     4561 tcgtttacaa atcggcttcc cagcaatctt tgcagaaaaa aacaagagat tttgattctt
     4621 acaaataaac cgcaagaagt ccaattgctt tggcagccaa aagaaagagg atgccatgaa
     4681 acattaactg tcatttttac agcctatgat agtctaggac ttttcagaaa aagtgcaaaa
     4741 agagagatct cttttcctgt gactgttctg cctgcttttt gtaagattca agcagaaaaa
     4801 ttacagagag ttctggaaaa aaaacagcag ctgaacgcgt accaaagagg tcttgatttt
     4861 cgggaacatc gcgcttatcg tccaggtgat tctttcaacc gaatcgattg gaaattgtct
     4921 gctcatactc aagagtggtt ataccgtgaa tacgattatc aagaagaaga atgttctcct
     4981 cttttttgct tttggggttt gcccagtcca aaatttgaac ctctgctaga cttgtatttt
     5041 tctttatggc atttgagaaa agaaaagcag ccggaacttc tgatattagg caaccgcgcc
     5101 tttcatggca aagaccccgg tcatacctat tttgcttctt tggaagctgg cgatgagaaa
     5161 gcttttttcg cacacttgaa aaaatatgca aaaaaacaaa tcgtcctctt tgttcctgag
     5221 aattctcctg aagtctcaga agctattgaa acattgtcaa aagatcgaac ggtttatctt
     5281 gtttatttcg tagaaaaaca actcatggtc caagaaaaag acaagatatg ttcattgcca
     5341 ggaggtgaat ggattgacga ttaaacggga aaaatggctg cgtggcgttt actcctttgt
     5401 gatactaagt gtaggtgctc cgatatttgt agtagctaat caattgcaaa acagctacct
     5461 tcttatccta actattggtg tcctatgcct tattgtctgc tgtattccta aaaaatgggt
     5521 acgtttgctg ctttctttgc cagtaatgat aggttgtttg tttttatatt ttccttcgaa
     5581 gacattttcc atattatgga tcttacagtt tttttcgtca gtcacagagg aattcttcca
     5641 tctatcccaa ggtaaacttt attatttgcc agaaaagaca gctttttttc tcataatgtt
     5701 gctgatctat ttatttatta cattgaccgt ggattacgac tattggtacg caccctttct
     5761 ttctttgatg gcttacttca tgatgcttac cgtttttcgg aaaaaagatt tactgtacga
     5821 aatgattgcg gtagtaactg ttttgttcgt gtatctggca gcaaggcaat ttttctccat
     5881 ccggttgttg tcgggaaaca ctcgattcca gtcattgact acagttctgc ttatttgttt
     5941 cttaactttt agcgctatct tgcctttata tcttccgaaa gtacaccaat ctctcgagga
     6001 aacgagtgaa ccgatccgag aatatcttaa tgatgaaggg ctttatgaca cactccatga
     6061 gtatcaatta accggaagtg caagaacagg cttcagtgag aatgatgaac agcttggtgg
     6121 acctttatat gataatggcg aagttgtttt tacagcgaac caaaaaggaa atggctattg
     6181 gaagatcgaa aataaaaatc attacaccgg ttctggatgg atagaagatc aggacgcacc
     6241 catccaacca atccaagaat tccctgttga catcacgagc taccaaaccg ttatagaaga
     6301 caaagaaaac attacattga ccctaaaaaa acaagttttc ttaccttatc cttatggaaa
     6361 tactaccttg actgattttt cattagctag tggacaaacg ctttactatt tgcctgaaag
     6421 ccagcgattc attcctgtcc cgaatgaatc gacacgttct gcaactttac aaacagaaag
     6481 gttagttttt acgagagaag aattaaaaag cagtcagtta aaagctgatg atttagaaaa
     6541 tcagcagaca aatcttcaat tgccaaaaag tttgcctaaa cgtattgagg acttggcggt
     6601 cgaattgacc aaaaatgaag caactttaat tcgtaaagtc gaagctgtct aacagtattt
     6661 aaaaacaagc ggaaatttcc gctattccaa aacggatacc gggcatactc caaaagaaca
     6721 cgattatgtg gattatttcc tctttgattc aaaagtcggc tattgtgaca acttttcaac
     6781 ttctatggtc gtccttcttc gaacacttgg tatcccagct cgctggacaa aaggttttac
     6841 aaatgggctc agaacaggca cagataatca agggaatgag gaatacagca ttctcaatag
     6901 tcatgctcat tcttggccag aggtatactt tcctggattt ggctggcttc catttgaacc
     6961 aaccccttca tttgccaatc caatgggttc acaaccagtt gctgaaacaa ccgaaaccgt
     7021 ccagcctgct atcgaacaac caaatgaaag cgaaaatatg gcagaaacta tttccagtga
     7081 gagatctgag gtcattccat cagaagaaac aacactcaca ccagcttata aaatcgtctt
     7141 attcagcctt ggagtgtgtt tatccgtctt gttgttcttt ctattgttat tcagaaaatc
     7201 tctgcgctgg acaagttatc ttatctatct tcattttttt gcttctacta atacgattgc
     7261 atatcaaaaa cttcttaacc ctttggaaac attggtaccg cgcaaacccg cagaatcttt
     7321 gcaggaatat agcctgcgaa tagaacaaga attcaagcta gatctgcgtt tcattcgatt
     7381 aactactcaa tatgaagcag aaatatatgg acgacaagca gatccggtaa aattcgataa
     7441 aaaaacctat ttagaaataa cgcatataat aagaaaaatg gtatacaaaa aaattctgac
     7501 aaaagggagc cgcttcaaaa aataagataa aatttctgga gaatctactt ctggaatgcc
     7561 gagaaccagc tgattgagta tcgattactc gaaaaaaagg ttgtgacacg ttttttgtca
     7621 caaccttttt tcattattat ctgattttca atgtcaaagc atttagagct acaactaccg
     7681 tacttaacga catcaagaca gcaccaactg ctggacttaa tatgatacca attggcgcaa
     7741 gaattccggc tgctagcgga attgcaagaa tattgtaacc tgccccccac cagaggttct
     7801 ggatcatttt ctttcgcgtt tgcttggcta gatcaagaaa atgcaaaata tcttttggat
     7861 cgctgtttgt caatacgaca tccgcagaat cgattgcgat atctgtccct gcaccgattg
     7921 ccatcccgat tgaagcacgg gctaaacttg gcgcatcatt gatcccatca cccaccatga
     7981 ttactttttt cccttggtca aggtatttct ggacaacagc ttctttatca tctggtaata
     8041 aaccgccata atattcttcg atccctaaat aatctgctac tgcttgggca gcgtttttat
     8101 tgtcaccagt aagcatgaca ggagtgattt ttctttcttt caatgttcgg ataaattctt
     8161 ttgcttctgt ttttactacg tcacctaaag caacgattgc agctagatga ttttctaaaa
     8221 tcaggaagct gatggtattt ccttgttctt ggtaaggttt caataatgct tgttctacgt
     8281 ttaatccgag acgctcaact tctttttcat taactatttt cacttgttgt tttttgactg
     8341 ttgcctctag cccgactcct gaaagatttt ttagattttc agcttgataa ggcttgattt
     8401 catgttcttt caaataattc ataatgccaa cagccaatgg atgattggca ttcgcttcca
     8461 aggctcctag atattgaagg atttcatttt tattgaacgc atcatcaagt acttcgacac
     8521 ccgttacagt aaattttcct tctgttagtg ttcctgtttt gtccagcatg atcacatcta
     8581 gatcattggc ttgctccagt gcattgcggt ttttcaaaag taatccgttt ttagctgcta
     8641 ttgatgtgga acgagcaaca actaatggga tagctaaacc tagagcatga ggacaggcga
     8701 tgataaagac tgttaccata cgttctaatg cattaggaag atctgtcaag aacaaccagg
     8761 cgatgaatgc caggataccc acaattaatg cgacataaaa cagccatttt gccactttgt
     8821 cagataatga ctctagcttg gatttttctc cttgtgcttt tcgcaccatt tccataacct
     8881 tcgctagata accattttca ccagttccag ttacttcgat ttcgatcgtt ccatccccat
     8941 tgatagaacc gccaatcact ttgtcattgg cttgtttttt gattcctctt gattcgccag
     9001 tcacagctga ttcgtcaaca attgtttcac ctttcaagat tacaccatct gttggcattt
     9061 tatcaccaga ccggacgatc aaacgatcac cttcgtttac ttctttcaat gaaactgtct
     9121 cttcttttcc atctttcgtc aagcgtttca ctgattctgg cagtaattct gctagttttt
     9181 gtaaagcatt gctggcattc gacacagcat tcatttcaat ccagtgacct agtagcataa
     9241 taacgatcaa agtagccaat tcccagaaga aatccatgac gtgtgtatgt ggattgagca
     9301 gatttgcgat aaacgaatag acactataaa tatatgcaac tgtaatcccc atcgcgatca
     9361 gtgtcatcat agcaggactt ttttgtttca attccatctt agctccactt aaaaatggct
     9421 gaccgccata gataaatagt atagttgcta agatcaatac gagccaatca gaaccaggaa
     9481 acgttacttg aaatggaaat tccattccca tcattggtga aaaaagaatg attgggatag
     9541 ctaggataag cgatagccaa aatttttgtt tgaaattccc catgtgcatg gaatgatcca
     9601 tgccgccgtg ttcgtgatcc atgtgcatcg aatggtccat ttgtttgctg tttgctatat
     9661 ctccatggtt atgtcccatg tgcatggaat gatccatctg actatggtca tgtttcatct
     9721 ccatctgttt ttcatgagat tctgtacttt ctttgtttac tacttctttt ttatgctcat
     9781 gttttttctc catatcgttc ccattcattt ttactccatc ccttctctta ctttttcatg
     9841 tgtcgattta agcacaggga gttcaacaat acactgacgg aactaaacgc catcgctcct
     9901 cctgcaatga ttggattcaa aaagcctagt gcggcaaaag gaataccaat cgtattatag
     9961 acaaaagccc agaaaagatt ttgtttgatt tttctcaatg tcaacctaga caactcgatt
    10021 gtttgtgcaa tggatgccaa actgctattc atcagcgtca catcggctgt ttccatcgca
    10081 atgtccgttc cacttcccat tgccataccg acatcagcta atgctaaggc cggtgcatca
    10141 ttgattccat cgcctgccat accgactttt ttgccatctt tctgaagttt ttctacgtag
    10201 gctgctttat cctctggaag tacttcagca aagatatgct ttggatcaat accaacttgt
    10261 ttaccaatcg tttcggctgc cagtttgttg tctcctgtca gcataaaaac atctaatcct
    10321 ttattctgga gctgcttgat tgcttgtttg acttctagtt tgatttgatc agccacggca
    10381 atcaacccga tcacttgctt ttcatcagct aaaaacatga ctgtttttcc ctgctgttct
    10441 aattctaacg cgtgttcttg gtattcatca aatgataggt tcaattcaat taatcgttta
    10501 cgtgtacccg caaaatagcg tactccgtta atcgtaccgc tgattccagc acctgggtgg
    10561 gctgtaaaat caatcatatc gtatgttgct gcttcttgca gttttccgta ttccacgatt
    10621 gctttaccta gtggatgttc agaagcttgt tctaaagtat aaaaaataga taggacttct
    10681 ttagaaccga ctagatctgt aactttgggc tttccttctg taatcgttcc agttttatcc
    10741 aaaacaatgc tgtctaagtg agcagctgct tctaatgctt ctccgccttt aattaaaatt
    10801 ccatttcgtg caccaacacc cgttcctacc ataatagctg ttggagtcgc taaacctaat
    10861 gcacatggac acgcaatgac taatacagag acactatgga tcaatgcgag ttgccaatca
    10921 ccagtgatca aacctgtagc tatgagcgtg ataaacgcga tgactaatac gatcggaaca
    10981 aaaatccctg agatccgatc tgcgattttt tggattgggg ctttgctccc ttgagcatct
    11041 tccaccattt gaatgatttg cgctaatact gtctgcttgc cgacttgtgt aacttccatg
    11101 tgaagtagac cattcgtatt gattgtcccg ccaaagagcg tgtcatccgg attcttttct
    11161 acaggaagat tttcaccagt aagcatactt tcatctatcg cactgcttcc tgaaataatt
    11221 cgaccatctg ctggaatctg ctcaccagga cggatgacta gttgatcacc aacgactact
    11281 tcttcaatcg ctaacgtttc ttctttcccg tttcttatca cttgggcggt ttttgtttga
    11341 agagacatca attgtttgat tgcattacct gttttcgttt ttgctgtatg ttccaaatat
    11401 ttgccgagca aaatcagcgt gatgatcata ctgctgcttt caaaataaag atcttggggg
    11461 tgtccacgga aaaatccgtt gtaaatgctc aatgcaaagg cagccgatgt tccaattgct
    11521 accaatacat ccatatttgg tgctttcgtc ttgattgcat ggtacgcacc tttataaaaa
    11581 cgagcaccga cataaaattg gacaggagca gacagcacta actggacgat tggaaaatga
    11641 aaaaagtgaa caatcgccgc atggcttcct aacatcatag caatcattga caacatcaac
    11701 ggcaaagtca aaaccgtact caatataaga tcgaatagca tttttttcaa atacgcttgc
    11761 ttttcttctg caattttttg tttgtgggct tcatcgtaca aaattgcacc atagccgatg
    11821 ttttctacgc tttggattaa tttttcagtg gtcgtccctt catatttgac acttgctttt
    11881 tcagtggcca agttgactgt ggcattgacc acaccaggtt gtccgttcag ctctttttcg
    11941 atcctagcag aacaatttgc acaagtcata cctgtaatga cgaacgtatc cattttggtt
    12001 tctgctgttg ccatcaaatc acctctgctt tataacccaa tccattgatt gcttgacaaa
    12061 tcttttcgga ttgaactttt gtttcatcaa acttgaccgt accgtttgct tttttcaaat
    12121 ttactttcac tttttgcacg ccgtcaagtg cgcttactgt ttcttctact cttgctgcac
    12181 aatgattgca gctcatacct tcgattgaaa attgttgttt cattttttct tcctcctatt
    12241 gatgttgttt acattcacat tgacctggga tacagttaca ttcgatcgta tctactggtt
    12301 gcttttgttc cagttgtttc aaaatctcat tgatatcttc ttgagtcaaa gtggcttctt
    12361 ctactagatc cgcaatcgtc tctccaatcc tttttgcaca gatatgggaa aaaagatttt
    12421 ccgtcgcact gcgaactgtt tccatctccg agacagctgg ataataaatg aatttttttc
    12481 cttcttgttc tgtccagatc acttcttttt ttaccaaacg acccagcaaa gttttgatcg
    12541 ttgctggttt ccaatccatc gaatcagcta agatctgcgt gatttcttgg gctgtcgtct
    12601 gtccaagtgt ccaaataaca cgcatgatct cccactctga atcactgatc tttactggcg
    12661 ttttgacttt ttccatttat ttcacacctt tcgtttacaa atgtaatcat tatcttatga
    12721 atacagatta cacttgtaaa caaaaactgt caattatttt tgctctctat tttcaattta
    12781 gttaaaaatt cttgtttctc tcgtcttgtt ttaaggatga tcagtttatt tcttgcatta
    12841 gctacttgga tcagctgctg tattttcggt tcattgtttt cttcaaaatg ccagataaaa
    12901 gataaaaatt ctaagtattc cctgtttagt ttctccgaaa atgattcagg catatctggc
    12961 cgagattttt tgtttatctt tctttttaga gaacgccaaa ctactcggta gatggcgaga
    13021 taacgcggca ctttaagcca aatgatctta tctgcttctt taatcctttc ctctaatgta
    13081 gaagtataat ttccttcaac gatccatgac ggattagata ccataaacaa ttgctgttct
    13141 tgcaaaaacc acttttttgc ctgaatactg tagtctgttg tatgccataa aaggtctagc
    13201 ggcagtagcg gaaagtcggt gatttctttg atcttccttg cgagggtcga ttttcccgac
    13261 ccactttgtc caatcacgat gatcttcatt cttctccctc acttctcttt ggaatttggt
    13321 tcgatcatac cacttataac gccgcaaaaa aacaagggac tgtgacaaaa gtcgtgtcaa
    13381 agtcccttgt ttcgaataa
//
LOCUS       DARBEC010000077        13141 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.080, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000077 DARBEC010000000
VERSION     DARBEC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13141
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.080"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            415..621
                     /locus_tag="SCL80_002147"
     CDS             415..621
                     /locus_tag="SCL80_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEG1107995.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(823..1824)
                     /locus_tag="SCL80_002148"
     CDS             complement(823..1824)
                     /locus_tag="SCL80_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1107996.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(1829..3742)
                     /locus_tag="SCL80_002149"
     CDS             complement(1829..3742)
                     /locus_tag="SCL80_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1107997.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLDTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(3910..4416)
                     /locus_tag="SCL80_002150"
     CDS             complement(3910..4416)
                     /locus_tag="SCL80_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1107998.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            4576..5016
                     /locus_tag="SCL80_002151"
     CDS             4576..5016
                     /locus_tag="SCL80_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1107999.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            5175..6515
                     /locus_tag="SCL80_002152"
     CDS             5175..6515
                     /locus_tag="SCL80_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1108000.1"
                     /translation="MSYSEFTKELLDILDLNLTFHEDAFRKERINDETCFVFDGTLTY
                     QPEECFHCHYQNKQTIIKWGWKKVSILLNDVSNYKTIVRINKQRFKCKHCGKTFLAED
                     SVSDRRCSIARRVKQAILELLSEPISMSLIARMKHISPTTVIRILRSLRPKTVSLNQP
                     LPEVVCFDEFKSVKNVSGAMSFVMMDGKTHQLIDIVENRQLNPLRDYFLRYPRKVREQ
                     VRLVVSDFYTPYRTLVKECFPNARIVADRFHISQHIGRAFTNHRIQVMKTFKKGDRRS
                     KHLKKYWRLLQKNAWELNGQHRYWRPSFRDHLTEAEIVDRLLYYDDSLKRGYEVYQVF
                     LSAIRRQDVPEFVALLKEDYKELPEHYQPVFTTFKKYRTEIKRALRVPYSNGPIECLN
                     NHIKVLKRIAYGFRNFQNYRERIFLYRGKYFKKTKNTTQLTKARTTTRLDKIAV"
     gene            complement(6584..7741)
                     /locus_tag="SCL80_002153"
     CDS             complement(6584..7741)
                     /locus_tag="SCL80_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108001.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(7744..8100)
                     /locus_tag="SCL80_002154"
     CDS             complement(7744..8100)
                     /locus_tag="SCL80_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108002.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="SCL80_002155"
     CDS             complement(8398..9372)
                     /gene="sgrA"
                     /locus_tag="SCL80_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEG1108003.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSESSTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(9568..10407)
                     /locus_tag="SCL80_002156"
     CDS             complement(9568..10407)
                     /locus_tag="SCL80_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEG1108004.1"
                     /translation="MIRIIRLIGSVLIVLVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            10592..11479
                     /locus_tag="SCL80_002157"
     CDS             10592..11479
                     /locus_tag="SCL80_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1108005.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(12063..12758)
                     /locus_tag="SCL80_002158"
     CDS             complement(12063..12758)
                     /locus_tag="SCL80_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HEG1108006.1"
                     /translation="MEIMLDTANTKSIYSCQQFLNLTGVTTNPTILKKEGASNFFSHM
                     KEIKKIIGQIPIHIQTIGRTEEEMIEDARVIIEELGKDTFIKVPVNQAGLQAIKKLKK
                     EGYLITGTAIYTELQGYLAINNGADYIAPYYNRMQNQGIDAMKVIDSLRTEIERTQRP
                     TKILAASFKNCEQVTTAIRSGAHAITLGKELLDQVLEMPSIDKAVRGFQEDWVQTFQA
                     ETIGELKKVADYF"
     gene            complement(12742..13140)
                     /locus_tag="SCL80_002159"
     CDS             complement(12742..13140)
                     /locus_tag="SCL80_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEG1108007.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT         3937 a         2646 c         2034 g         4524 t
ORIGIN      
        1 attatttttg tttcttattt tctaaaaaat taaaaaaaat tttggtgaat taagtatttc
       61 cttgataaca ctggcttttg gcctcatttt aggttatcat ttgtaatttt atttcacagt
      121 tgatatgatt gttatgtaaa ataaaaaagt ttaggataca tcgaattttt ggggaaattc
      181 ttttgggggt atcctaaatc ttttctatta tattcccata ttttctatta ttggtctaga
      241 cgtatgatgg taaactttgt acctatctga ttgctgaatc attaattgct tatcattagc
      301 catctttgtt tcaagttcgc tagatgatca ttttattcac atcctaacaa acttgtcaca
      361 tttatagatt cttaatctat ttatcataca attataaaaa aggaggtgct gcttatgaaa
      421 gctatccata tttctttgct ttttttcgct agtgcattga ttttatttgc tattcatctt
      481 tgttcccagc cttatatcaa taacaacgga atattagtgg aaccagcatt cttttgctta
      541 ccactcggct ttttgtccct tcttgcctcc gcaggcttcg gatttgttgc tttttttaaa
      601 cgatcaaaac aacaaatctg acttataaaa tttacgtaaa agagatcgtg acaataatcg
      661 tgtgatattc ccaaatttcg aataaaaggt gccccttgcc atctctaact cctcagtcac
      721 aagttacaat attcataaac catgaaaagc ttgtttattg aaattgctcc ttgtgacttg
      781 gagctaagtg acagattaca acctcttttg catcataaga ctttactcct tgaaaaacat
      841 tactaacttt tgatatgttt cttgcgcttg tttattgctg aaatcaaact gatattcatg
      901 gttcaaaaca gcatttttct ccgcataatc tacttttgtg actttcaccc ccgctttctc
      961 aaatacagaa gttacttctt ccatttgttt cgtaaacgta tttgtatttc catccgttaa
     1021 gaatgtgttt tgcgtccaac gatccgcatg tctgaccaag gctaaacttt tgaccttctc
     1081 tgattgttca aagtccttat tatcaaagta agcccatcca catttttcat aaagaaagct
     1141 agagatttta tcacttactt gagtaagtgc agaaggatcc aacagaccag acaaagaaat
     1201 aaacttcgta atctgcttgt tctccaaagt cggatcaaga tgatcagctt ttttaatttc
     1261 ttcattttga agtgctaaca aatattctcc actaatttgt gcacctgccg aatttccacc
     1321 aatcgaaata ttcgaccaat ctgcttgatc ttttatctct tttttagctg cttgaatggc
     1381 ttcgttcaat tggataactg gtgtaggatg ttttgcttcc ggtgatcgtt cataatctat
     1441 atttacgatg atttgctgcg tgtctgaaca tagatattcc aaataatcac gacaatcatt
     1501 tttatcccct cctacatatg cgcctccatg catccagaaa agaatcggta attttttctt
     1561 ttccactttc ggtgtgtaga tatccatatt attatttttg tactttgaag gataggatat
     1621 ttcttttttt atatcgattt gttcgatttt ttgctggaaa tcatctggtc gggcatatac
     1681 ttgttctttt tcacttgtgt caaattgttt tctaacaagc aagctaatcg gctttggtgt
     1741 gatttgatta atcaaaaata cagtgactaa aataactacg atgataactg gtactgcaat
     1801 cactattcgc ttgaaccatt tcattcattt accctctctt tcttcgccaa atccatgtga
     1861 taatcgtgct aacagataaa aatccacctg aataaatgag aagcgaatgt ttcttttctc
     1921 cagttttcgg caacattctt ttttttgcat ctgtcttttt ttgtactttc actgtcatct
     1981 gaatcggttg cgtatcatca cttgattgat cctctcgagg ttcattcttt cccttattgt
     2041 ctgcattctc tgtcggatct tgcttttttg gtggagctac tgggattttt ttgtaataga
     2101 aaataatagt tggtatcgtc tcattaaaaa tcccttcagt cggtccttca acatttgcta
     2161 attcgtatcc ttcgaatgtc ttttgctctg tcatataatt ttctccataa gctccgtaaa
     2221 gctggatttc gtcctggtct ttgagtagac ttttattgtt ttcatccaca tactttacgt
     2281 ttatctgatc atggaacact actttcaaaa agtccgttac atgtttcgtg taggcagcat
     2341 ggtcaacttc ctgtgccttt ccatgccctg ctccttctac taataaaaga tctttatacc
     2401 ctcctgataa ctcatataat ttttgcgcgt tagacacagg tactacatca tctgctgttc
     2461 catggataat caatttaggc aatgaatctg acattccaat cgtaggtaga gcagcttttg
     2521 tatccaaatg catctcctga tcaaaatagt attgtttcaa aaggcccata aataaatctt
     2581 catgctcttg atccccaatg atgtctaact gattttttat ggcatccggt agtgcggggg
     2641 ctactgtctg gctgtacgta aaacgtagtt gttcatcaat tgatgcaaat ccgcaatttt
     2701 caataattcc tttgacattg acaggaatat ctttcgctaa tacagacatc atcgtagctg
     2761 ctcccatcga accaccataa agtaaaatct tttgagatgg ccgctctcga acttcttgat
     2821 ttatccagta aaggacgtca tctgactcat attgtccata ggtcacataa tcgcctccgc
     2881 tattaccagt cgctctggaa tcaacaaata ataaattata cccggcctta taaaaatcat
     2941 tgtattcttc tgtaacgatc ccgttattcc aattccctcg aaatccgcca tggatcatga
     3001 ctgttttatc tgaaccttga tcgacataat atgcatatac atccatcatt ttgccatctt
     3061 gtttgtttag tacagtttga tgaagttgtt ttgtatttgg atagaagcct gtgttttctg
     3121 taggcgttgt cccatcatct gtacctccaa gcatcgacat caacacactg ttttttgcaa
     3181 aagcgatatc gtacaataga taagtagcaa tcagatgttt gataatctct ttatctacac
     3241 cataatcatt aacagcaagc tcagtgacta ataactctat taattttgac ttgatgtctt
     3301 ctgttggtcc attaagtatt tctgtcatct gtttaccgaa gtaattcaaa tattctttac
     3361 cattgaaatt ttcaccttta ccgatatcat cattttctgt cccattctct ttgtcactta
     3421 ctttagcaga agaggattcg tcgtttgaca tttttgtagc tgttgcttct tctgtttgtt
     3481 ggttatctgt ggtagcggtc gtttcttgat ctgtgctagt aacagatgtc tcaaccgttg
     3541 tttcagtatt tgtttctgtt tgtgaagtac tcgatttcgt ttcatttcca gcatcagtag
     3601 tttctgtggt ttctacctcc cgagattccg attcggaaga aatgctctct ccatatactg
     3661 tccattgcgg gacgcttgta cttacagcaa ataagatgac caacgctccc tttaatactt
     3721 gttttgttct caagctattc actccttttt atgtttctag gagaattata tgtaaacgca
     3781 aacatttttt atacaaaaaa taaatggttt atcccataaa aatagaacag gattattttc
     3841 tcattttacg taataaagca gaatggataa accagtatcc attctgcttt ttttaaacga
     3901 agaaacaact tattgctcca aaaatgtcag aaatcgttta tcccaaattt tctcgtaaaa
     3961 agcgattgtt tcctttgctt tcataaaagg attatcataa gttgtcgttg ctcctctaca
     4021 catcataagc tggtatccta atccatgagc aaacttcaca gtcgtatcta tacaatactc
     4081 tgcttgtgcc ccacataatt cgatcgaatg acagtccaat tcagttaata aaacactcaa
     4141 attagtctta taaaatgaat tcgcatgggt ttttctcaca aaataactgt gctcaggaat
     4201 agataattcc gacaatagct cccatggttt tgtttgtggc actaattgag catcttcgtg
     4261 ttgaacgaaa ataactggtt tcccaatcgt ttcgtatgtt ttgattcttt tattcactaa
     4321 tgctgtcact gtttctaatt cgtgtatcgg cgaagaatca aaacataccc ctttttgtaa
     4381 atcgatcact actattgcgt cggctgtgtt tgacatactt gtgccttctt tcttttttct
     4441 tgatttaata gtatttctta tctagctagt tccattatac gtgacaacat tttcttattt
     4501 tacacctcta aatggcactt ttcgactatt tcttcatgtt attatttcat aaatacttag
     4561 ctattggagg aatgtatgat gattaaattg tatacaggtt ctggctgctc gtcttcaaga
     4621 cgcgcaaaac gttggcttat cgtccatgat ataccgttta tcgaacagaa cttaattcgt
     4681 caaccactaa ccaaaaatga atttatccac ttactaaaat tgacaaacaa cggcgtgacc
     4741 gaaatgctct ctactcattc tattagttat aaaaatcttg ttcacagaat agatgaactc
     4801 agtttgagtg aactatatat tgagattcgt acatcccctt ctttattgaa aaaaccgatt
     4861 atctacgacc aatctgcaaa acttttgact ggatttagcg aagataaaat caggatgttc
     4921 ctttctaaaa ctaaaagaaa gcaagagctc aacgaactct tgctttctgg tcatcttgtc
     4981 gattttaaag aaaataatct ttttgcaatg tcttaatttt ggcataaaca atctgctttt
     5041 ctggatctat aatatctagt gttggtgacg ccttctgttg ccaatacttt ttcttttttc
     5101 taataaacgc aaacagacac cttgaaatcc tttagaataa agtcgacgaa aaccattcaa
     5161 aggagtgttt caagatgtcc tattcagagt ttaccaaaga gttactagat attctagacc
     5221 taaatcttac ctttcatgaa gatgcctttc gcaaagagcg cattaatgac gaaacatgtt
     5281 ttgtttttga tggcacattg acctatcaac cagaggaatg cttccattgc cactatcaaa
     5341 ataagcaaac gatcattaaa tggggctgga aaaaagtatc catcttattg aatgatgtca
     5401 gtaactacaa gaccattgtg cggattaaca aacagcgctt caaatgtaaa cactgtggaa
     5461 agactttttt agcagaagat tcagttagcg atcgtcgctg ctccattgcc cgcagagtca
     5521 aacaagccat tcttgagcta ttaagtgagc ctatttctat gtctttgatt gcacgtatga
     5581 agcacatttc tccgaccact gtcattcgaa tacttcgcag ccttcggcct aaaaccgttt
     5641 ctttgaacca gccgttacca gaagtggtgt gtttcgatga attcaaatcg gtgaaaaacg
     5701 tctcgggagc catgagcttt gtcatgatgg acggcaagac ccatcaactg attgatattg
     5761 tggaaaatcg gcaactcaat cctttacgcg actatttctt gcgctaccct agaaaagtcc
     5821 gtgaacaagt acgtctagtg gtttcagatt tctatacacc ctatcgcaca ttggtgaaag
     5881 agtgttttcc gaatgcccga atcgtcgccg atcgctttca tattagccaa cacatcggtc
     5941 gggcctttac caaccaccga attcaagtaa tgaagacctt taaaaaaggg gatcgtcggt
     6001 cgaagcactt aaagaaatac tggagactac ttcaaaagaa tgcgtgggaa ttgaatggtc
     6061 agcatcgcta ctggcgtccg tcatttcgag atcatctgac agaagcggag atagtcgatc
     6121 ggctattata ttatgatgac tccttaaagc gaggctacga agtctaccag gtttttcttt
     6181 ccgctatcag aagacaagat gttcctgaat tcgtcgcgct cctaaaagaa gactacaagg
     6241 agttgccaga gcactatcaa ccagtgttca cgaccttcaa gaaatatcga acagaaatca
     6301 aacgagcgtt gcgagttccc tattccaacg gccctataga atgtttgaac aaccatatca
     6361 aagtactaaa acgaattgcc tatggcttta gaaactttca aaactaccga gaacgaatct
     6421 ttttatatcg aggaaaatat ttcaagaaaa ctaagaacac tacccaactg actaaagcca
     6481 gaacaacaac acggctagac aagatagctg tctagccgtg tgagtatctc gattattcta
     6541 ttgaatttct tcaccaacac tcattgacga agagccgctt ttcttaatta acgaaatcca
     6601 atttttccaa tatggatcta tttttctcat tcctgatttt tatgatttca gctttcagac
     6661 gcgcccaatc attttttgta ggttgtccat tccagtaaaa tatctgtgta ttcgtttgat
     6721 catatgcctt tttgtcgata ggataatcgc taatgacaat atcaggtaac tgatcagcgg
     6781 atgcactata ttttattggg cgttcagtca tagataatat ccttttcttt gcccataatt
     6841 cctgtgtttt accatacttt gattgaatat agaatatgac aggttgttcg tgttttataa
     6901 aaatatccct gactaacata cagtattgaa aaatcaaccg tctgttcttt tcataataag
     6961 tattaagctc tggaattctg cgagctaccc cactaaaaaa ctgttgcatg attggataca
     7021 tcactggaaa tatatgctga aaatctctgt cttctgctga ttttccaaaa gcatccacct
     7081 tcttttttgt cccaaaaacc cttgaataca aatggatcat tgccaaattg acatatagat
     7141 aatttttttc tgaagttgta aaacaaatct tgaagttttt ttcaaaattt tctacaatag
     7201 acgattcaat ccagccccaa ctttcttgta ctttatctgg agtatccaac aaatcttctt
     7261 ccaaataggt gttcattaca ccaaacataa aataaaggta agcttgctct gttggagtga
     7321 tgtgattcat cgaccagaag tttgcttcaa aaaaaggctc tagtatgtaa tgcgtaaact
     7381 cttcttttgt tatcaatgga tgcacaaatt tgttcatctc ttctgggaca tacgtaatcg
     7441 agtggccttg tttcattcgt tgcaacgaaa ttcttagtag gctgatccat ttctttctat
     7501 ctatactacg cgtatatggc gcatgtttcg caaatttttc taatagttga cgtttatttt
     7561 ttatacttga taatatatca gaatgatttt cgtcgaatga ctgccaatac atcaagtgaa
     7621 aaaagtatcg gatacggtat tcttctcctt ctatcggatt accttttttc aagctgcaag
     7681 ataaatgata gtttttcaaa atagtattaa gttctcttaa ttttctttga atcgtacgca
     7741 aacttatata attttttgac taagattttc taaggattga taagtacctt tgtaaagtga
     7801 atccagtaaa atataaccaa tcgttccctc taaaagtata gaataaactt tactcagtaa
     7861 aaactgagaa gaaaactcga cttttatcat cttgttttct gaaagaaaag ataatttata
     7921 ttctggatac ctttcgcaaa acgattctgc ttgttgaatc accgaattca tcttagccag
     7981 ggtgtagttt agatctaaag cgatatcctt caccacatat aatttctcgt ctctttcata
     8041 acaatagttc aaaaattgat atacttgcct ttcattgata tcaataaatt cttcaaacaa
     8101 agtacatact cctttttatc tattatttag acttattccg acagctacat gaataacgta
     8161 caaatagatt cgttactgat ttttcagaga tactaccatg cgcagtgtgc attgtcaatt
     8221 attcttgctc ttattttcca tttgtgttcc tgttatttcc tgtttctcaa atatgttagt
     8281 aaacaaaaat agatctggca aagctacaac ttcgccaggc aatacagcac tctcgaatga
     8341 aatattcact caaaaatcta tcgttttatt caattttaga tctgttgctt ttattcttta
     8401 tttcactcga tggaagagag aacgaaaata gcttgctcct acaatcaaaa tgagtaacac
     8461 acccatccat gtaattagct tgttcaaggt tctactacca gtttctggta aatcgctttt
     8521 tctattatct gtttctttcg ctttttcttg atgatcaatt ccacgagaaa caaaatcagg
     8581 ctgttttagc acacttttta ggttgctccc tgcatttctc ttttgttcct tagttggtat
     8641 gataggtacc aaatcaagtt cattatttac ttttcctgtg ctgctttcgc tgctcgtgct
     8701 tcctgtgcta ctctcactac tcatgcttcc tgtgctactt tcactactcg tactctccgt
     8761 actgctttcg ctgctcgtgc ttcctgtgct actttcacta ctcgtactct ccgtactgct
     8821 ttcgctgctc gtgttttctg tgctactttc actgctcgta ctctctgtac tgctttcacc
     8881 gctcgtgttt tctgtactgc tttcactact tgtgctatcc gtactgcttg gcggtccaac
     8941 aactgtccaa ctgcttggtt tcaaattctc cgtgatcgtc atctgtccat cataaaaaac
     9001 aatcaaatca actttatctt tactcttgat ctcatcaatc gatagttctg cagaaatcat
     9061 tattgtatac tcaataccag cggtccattg tacgtctttt aaataaactt ccccaataac
     9121 tttttctgaa cttccattac catcccaacc atatgttaga acagcatgta catcactagc
     9181 attcacgctt ttgtttggtt tgactggaat agtaatgact tgttgtgtca tgtcataaac
     9241 aatccgatca gttttcacag taaatgaaag attctcattt gcccgttcat ttgcagaagt
     9301 actgattcca atcattgctg caattatact cagcccaaga ataacaaata atacggtcgc
     9361 tgttttcttc atttctatca ctctccatat aattaccttc tatttgataa aggaattata
     9421 taaaatgaaa atgatgagaa cacgacagtc agttgtgaaa atctaacaaa agatgtcgtg
     9481 tattcgcttg aatttgttta ctttttcgtt taatcaatga cagacttaaa aaagcaagac
     9541 cgatatcagc cttgctttct tcatctttta ttgataaaca gcagtgtatt tcttcatccg
     9601 ctttgtttta tatgcataga gtaacaaacc aacaatcaag actaacaaga ataatgtagt
     9661 tgatatagat gcttcagccc caaagtttcc cccagtaatt attgtatttt gcgattggat
     9721 cagagtggag aaaaaacctt gttcttgcgt tccgctgact ggtataccca aaatagctcc
     9781 ttgtgtccaa ttccaagtaa tatgagcacc aatagccgta aataaacttc ctgtactgta
     9841 gataagtgcg ctgaaaaagc aacctgctaa aaagatattg aaaactgcca gaaatgtcat
     9901 gttaggattc aatccgtgca aaagagcaaa taatgcagca gatccaatca ttgctattaa
     9961 cggaccaaat ctaagcatct gatgctgaaa aaagccacgg aataaaagct cttctgatgc
    10021 ccccactgca ataaataaaa taaaactcgt tatcagcggc acaatgtttg cgtttcctga
    10081 atctaaccga tacgcccccg tcaaatataa tggactagta ataagcagaa acactccaat
    10141 tccaacaacg agacctcccg ctaaataccc ccagtctttt cctcgaacag aaacaccata
    10201 atcttctatt ctttttcctt cgaccttact agcaagaaat aaagctagta aaaaaacagc
    10261 taaatgtccg accgtaccgg caattgctcc aatccattca ctatttccaa taaacatatc
    10321 cgcaagtgca ttattgacaa gaattcctaa aacaaaacca actaagacta ttaaaacaga
    10381 tccaatcaac cgaataatcc tgatcatctt tcatccctcc tttatattaa gtttaacaac
    10441 ccttttcttt cttgtctcgt gaaaactatg taaatgttct ccatcataga gagaatagat
    10501 ttttacgaac taagaatatc tacagcaacc ttacgaaaat gaaagactgt ctccctaatt
    10561 cttctgttag actgttttta ataaggaaag gatgggaaat tttttgaaaa aacaactact
    10621 aattatgtgc ctttcagcat gtgcaatttt actattctca ggttgtagca agtccaatga
    10681 aacgattgca acgtttaaag gtggaacact tacagttcag gatttttatg ataacccaaa
    10741 agttaaatca aataatcaaa cactattaaa aaaaatgatt gtcttcaaag catttgatga
    10801 tgtatacgga aaagagattt cttcaaagga agtaacaaaa gaatataatg aacaaatcaa
    10861 aaaactcggt ccaaactaca aggaacaatt aaaagcagtt ggccaaacag aagagacata
    10921 taagaaactc tttaaacaga tgctggcttt ccaatacggt ttgaaagcca atgtaaaatt
    10981 aacagataaa gatctagaca ctgcttggaa agaattctat ccagaagtta gtacacagat
    11041 cattctattt tcgactaaag aagaagcaga taaagcaaaa aaagaagcaa atgaagggga
    11101 aaatttctcc aagcttgttc aagcttatgg aaaaaataaa acattaaagg aaactgatgg
    11161 aaaaatgaat tttgattcaa ccaatccaga gattcctact gaagtaaaaa aagcagcatt
    11221 taaactaaaa aacggggaag tttctgatat cattcctgta acagatccaa cgacttatca
    11281 gcaaagctat tatctagtaa aaatggtaaa aaaacaggat aaaggttcta ataaagacaa
    11341 atacaaatct gagctagaaa aaattgctac cgaagcaaaa actcttgata cggaattcat
    11401 ggataaaaca atccgtaaag tgatgaaaaa agacaatgta actattaaag atccttatgt
    11461 aaaaaatata ttcaaataaa caaatttgac ccaattgggg atgtggcaaa atttgagaag
    11521 tgatttttgg aaattccttc ttatttttga ggcaaaccac ttttgccaca acttcatcgg
    11581 ggcctttttt tatttgtacc aaaacaaatc actggatttg ttacaatcat gaggatataa
    11641 tttaaaaaag ttttttattt ctatcacatg aatcaatgcg ccattaagta gattctttca
    11701 tgcttcccta ttttttcact tgccgtatcg cttcaaaaac aactatcata aactcaaact
    11761 cgactgaaaa gttattttaa aaaagactaa aaatatccct tctggacttg ttcctcttat
    11821 tctgttatac tagagaaaca cgaaagatat ttttatgcga cacttttcat ttgtttttat
    11881 gaaatttatt caaaactggc gtaacttcag agttacaagg atgtattaga ggttagggaa
    11941 tacatcgttt aaatgagaaa taagtaatag tcacccaaac tttaaattaa tgaatattga
    12001 atcgtcagat agcctcctcg agttttcgag aaggctatcg tttattttat ggagaaattg
    12061 attcagaagt aatcagcgac cttttttagt tcaccaatcg tttctgcttg gaaagtttgc
    12121 acccaatctt cttggaagcc cctcactgct ttatcaatcg agggcatctc taatacttga
    12181 tctagtagct ctttacctaa cgttatcgca tgagctccag atcgaatggc tgtagtgact
    12241 tgttcacaat ttttgaaact agctgctaat atttttgttg gtctttgcgt acgttcgatc
    12301 tcagtccgca acgaatcaat cactttcata gcatcaatgc cttgattttg catcctgtta
    12361 taataaggtg caatataatc tgctccatta ttaatagcta aatacccttg taattctgtg
    12421 taaatagctg taccggtaat caaataacct tcttttttca actttttgat cgcttgaagg
    12481 cctgcttgat tcaccggcac cttgataaac gtatcttttc ccagttcttc tatgataact
    12541 cgagcatcct ctatcatttc ttcttccgtt cttcctattg tctgaatatg gataggaatc
    12601 tgtccgatta tttttttgat ttctttcata tgcgagaaga agtttgacgc tccttctttt
    12661 ttcagaattg taggatttgt tgttacgcct gttaaattca aaaactgttg acaagaataa
    12721 atactttttg tatttgctgt atcaagcatg atttccatat agctttcccc cattctgatc
    12781 ctcttgatcg attttaatga ttgtcccctt tcttaaatgc ggcatttcct tatcttctac
    12841 atacattgtt cctggaagtt ccggctcatg agatccatca aattttatcg tgatatgtcc
    12901 taattcattc aaattagttt ggacaacatc tcctactgct gtgattacat acatttgttt
    12961 atcaaaacag agcttcatat cacaagttat ttcttttctt acctgattca attcaatctt
    13021 ataacaataa tctgttaatg tttccggtgc ttcatctcca aataaaatca ttattttttc
    13081 tttcctgaat acttctgctt ctataccaac atctgttaca accgtttcaa agatcgtcat
    13141 c
//
LOCUS       DARBEC010000078        12868 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.081, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000078 DARBEC010000000
VERSION     DARBEC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12868
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.081"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            185..1183
                     /locus_tag="SCL80_002160"
     CDS             185..1183
                     /locus_tag="SCL80_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1108008.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="SCL80_002161"
     CDS             1295..1804
                     /locus_tag="SCL80_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEG1108009.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="SCL80_002162"
     CDS             complement(1834..2493)
                     /locus_tag="SCL80_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEG1108010.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="SCL80_002163"
     CDS             complement(2495..3259)
                     /locus_tag="SCL80_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEG1108011.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="SCL80_002164"
     CDS             complement(3267..3944)
                     /locus_tag="SCL80_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEG1108012.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="SCL80_002165"
     CDS             complement(3981..5366)
                     /locus_tag="SCL80_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1108013.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="SCL80_002166"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="SCL80_002166"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="SCL80_002167"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="SCL80_002167"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEG1108014.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="SCL80_002168"
     CDS             6305..7162
                     /locus_tag="SCL80_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108015.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYRYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="SCL80_002169"
     CDS             complement(7243..7578)
                     /locus_tag="SCL80_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108016.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="SCL80_002170"
     CDS             complement(7645..9015)
                     /locus_tag="SCL80_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEG1108017.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="SCL80_002171"
     CDS             complement(9093..9629)
                     /locus_tag="SCL80_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1108018.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="SCL80_002172"
     CDS             9938..11419
                     /locus_tag="SCL80_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108019.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLKKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            11584..>12868
                     /locus_tag="SCL80_002173"
     CDS             11584..>12868
                     /locus_tag="SCL80_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEG1108020.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKMSFICADGETGQLIDVLPTRKLPRLTSYFLGCANPEE
                     VKFLVTDMNAAYFQLTKRILPNAKVVIDRFHIIKHMNQAFNELRIREMNELRKVGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLGFSTSLKEAY
                     PYFHELVEAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITNAMIYPYTNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT         3995 a         2569 c         2123 g         4181 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgcta tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcgctatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaaaaaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccggc tctataattt
    11461 atgggactga tgaatcagaa ttgattcttc agccccattt ttattttagg tattaaaaaa
    11521 catatcgttc tctagtatga ttaaatcacg acaaaataaa caactgagag gacgatatgc
    11581 tatatgaatg attctatcaa aaaaatgctg cgattaatag agaaagattt gatgattaca
    11641 gaggtctctt acgagacctt tcagaagaaa aagacgttga tcgtcgatgc tgttttctca
    11701 cctgctcccc atacctgtag aaattgtggt tctactgtgg tagatggaaa cgggaaagta
    11761 attgtagtga aaaatgggaa gaaggaaacg attgtccgct ttgaacaata caatcacatg
    11821 cctttggtta tgcgcttaaa aaagcagcgc tatacctgta aaaactgtcg aacccattgg
    11881 acggctcaaa gttattttgt tcaacccaga cattcaatcg cgaatcatgt cagatataaa
    11941 attgcttctt tactgactga aaaagtatct ttatctttta ttgcgaaaaa ttgtcaggta
    12001 tctttgacca ccgttattcg tacgttgaaa gagtttaaaa gctacttacc aaagcaatct
    12061 aagaggattc tccccagagt attgatggtt gatgaatttc gttcgcacgc ttccatagaa
    12121 gataaaatga gctttatttg cgcagatggc gaaacaggac aattaattga tgtcttgcct
    12181 acgcgtaaat tgcctcgatt aacaagctat ttcttaggct gcgctaatcc tgaagaagta
    12241 aaatttttag tcacagacat gaacgccgcc tacttccagc ttaccaaacg tattctgcca
    12301 aatgcgaaag tagtgattga tcggtttcat attatcaaac acatgaatca agcctttaat
    12361 gagttgcgta tccgtgaaat gaatgaactt cgtaaagtag gacagaaaag tcaggcagaa
    12421 aaactgaaaa agaactggcg ctttttgcta aaaaatcgtg caaacatcaa ccattatgaa
    12481 tacaaaacat ggaaaagttt tcgagcacca aaatacccat ttcttacgga agcgatgatg
    12541 attgatcgat tgcttggctt ttctacgtcc ctgaaggagg cgtatcccta ttttcatgaa
    12601 ttagttgaag cctttcgaga caaagaccct gacttatttt tctccttatt ggcagaactt
    12661 cccgaaacgt tagatgacgg ctttcgggaa aagctccaaa accttctgac ctatgaagaa
    12721 ggcatcacca acgcgatgat ctatccttac accaatggaa aaatagaagc gaagaatacc
    12781 catataaaga caatgaaacg agtatcctac ggatttaaat catttgagaa catgagaatt
    12841 agaatctttt tgatcaatca attaatca
//
LOCUS       DARBEC010000079        12734 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.082, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000079 DARBEC010000000
VERSION     DARBEC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.082"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCL80_002174"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="SCL80_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEG1108021.1"
                     /translation="MAVLARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="SCL80_002175"
     CDS             complement(322..891)
                     /locus_tag="SCL80_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEG1108022.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="SCL80_002176"
     CDS             1077..1979
                     /locus_tag="SCL80_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1108023.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCL80_002177"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="SCL80_002177"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEG1108024.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCL80_002178"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="SCL80_002178"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEG1108025.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCL80_002179"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="SCL80_002179"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEG1108026.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="SCL80_002180"
     CDS             8547..8732
                     /locus_tag="SCL80_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEG1108027.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="SCL80_002181"
     CDS             complement(8794..9990)
                     /locus_tag="SCL80_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEG1108028.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVARAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="SCL80_002182"
     CDS             complement(10160..11596)
                     /locus_tag="SCL80_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEG1108029.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="SCL80_002183"
     CDS             11889..12698
                     /locus_tag="SCL80_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1108030.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3803 a         2674 c         2249 g         4008 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctagtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcac gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DARBEC010000080        12445 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.083, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000080 DARBEC010000000
VERSION     DARBEC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12445)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12445)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12445
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.083"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(60..857)
                     /locus_tag="SCL80_002184"
     CDS             complement(60..857)
                     /locus_tag="SCL80_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1108031.1"
                     /translation="MKVSQKIILSILLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEP
                     KSVPTTLNVALIGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGM
                     NKINAAYSYGGVDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSL
                     DLDGVYINKGEQVIKAISQQLKDVTSIFKLPKAVGKLLGSIQTNLPESVLLDCGMDFL
                     KDNNKKIDTLSVPVDGSWDFNDNTPSGSVLELDLTKNQEAIKKFLNN"
     gene            complement(949..2112)
                     /locus_tag="SCL80_002185"
     CDS             complement(949..2112)
                     /locus_tag="SCL80_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEG1108032.1"
                     /translation="MKKRKKIDWWILGPYLTLSMIGLLEVYSASSYRLLQADENTKSL
                     LLRQLIFIFLSWSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGA
                     QRWISLFGIQFQPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPK
                     IAGALMILSIAWVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNH
                     AYERIATLRDSFIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIF
                     SIITEELGLIGALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVG
                     SIAGLMPMTGVPLPFVSYGGTSYLILSLGIGITLNISSKIQAEELPLYRPEKQ"
     gene            complement(2629..2835)
                     /locus_tag="SCL80_002186"
     CDS             complement(2629..2835)
                     /locus_tag="SCL80_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108033.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2851..3465)
                     /locus_tag="SCL80_002187"
     CDS             complement(2851..3465)
                     /locus_tag="SCL80_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEG1108034.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETKEAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(3612..4295)
                     /locus_tag="SCL80_002188"
     CDS             complement(3612..4295)
                     /locus_tag="SCL80_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1108035.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RSNTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(4404..4862)
                     /locus_tag="SCL80_002189"
     CDS             complement(4404..4862)
                     /locus_tag="SCL80_002189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEG1108036.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4946..6709)
                     /locus_tag="SCL80_002190"
     CDS             complement(4946..6709)
                     /locus_tag="SCL80_002190"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEG1108037.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6930..8390)
                     /locus_tag="SCL80_002191"
     CDS             complement(6930..8390)
                     /locus_tag="SCL80_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108038.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(8598..9524)
                     /gene="glsA"
                     /locus_tag="SCL80_002192"
     CDS             complement(8598..9524)
                     /gene="glsA"
                     /locus_tag="SCL80_002192"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEG1108039.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(9547..10974)
                     /locus_tag="SCL80_002193"
     CDS             complement(9547..10974)
                     /locus_tag="SCL80_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108040.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(11005..12219)
                     /locus_tag="SCL80_002194"
     CDS             complement(11005..12219)
                     /locus_tag="SCL80_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEG1108041.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
BASE COUNT         3753 a         2561 c         2057 g         4074 t
ORIGIN      
        1 ataaaagaag tcagaacata acatagattg ttatttctaa cttcttttat tttctttact
       61 taattattca gaaatttttt gattgcttct tggtttttgg tcaaatctaa ttccagaaca
      121 cttccggaag gcgtattgtc gttgaagtcc caactgccgt ctactggtac ggataatgta
      181 tcgatctttt tattattgtc tttgaggaaa tccataccac aatccagaag cacactttct
      241 ggaaggttcg tttggatact accaagtaat ttcccaaccg ctttgggtag cttgaatatt
      301 gaagttacgt ctttcagctg ctgagagata gctttgatca cttgctctcc tttgtttata
      361 tatacgccat ctaaatctag agatttttct gcatcgatct tgactccacc aggaaacagt
      421 tcattaacgc aatcgataaa atcttgaaat gtgatacttg cataataggg agcttcaaat
      481 ttgaaatttt cctttaatgt ttgatttaat aaatcaactc ccccataaga gtaagctgca
      541 tttatcttat tcattccgta acctggaata tcgacatatg aatctctcat gatagagatc
      601 agttttgctt gttgtgtttt ctggttgtat tgtgcaacca gaagtgaatc cgagcgaccg
      661 ttttcgtctt tcgaacgagc atctgaacca atcaaagcca catttagtgt ggtagggaca
      721 cttttcggct ctaccttttt cacagttgaa ctctgttttg cagacgaaaa tccatggata
      781 aactcaatcg aaaaatagcc aactactaat gtaagcagta acaaaataga taatatgatt
      841 ttttgagata ctttcattgc gaattcctct ttctaaaaga acctctcgct tgaaaaataa
      901 aagattgtac tttatgataa ttcaaactga gagtttctca gtttctcttt attgtttttc
      961 aggtcgatag agcggcaact cttcggcctg tatctttgaa gaaatattca acgtgattcc
     1021 gattcctaaa gaaaggatca gataacttgt tcctccgtag ctaacaaatg gcaatggcac
     1081 accagtcatt ggcatcagcc ccgcgatact tcccacgttc attatcgtct gaacgaatag
     1141 caaagttccg aatcctaaaa gaaacaatcc agcttgctga tttttacagc gactgcttaa
     1201 acaaaagatt ctcatgcata atgagaacaa taagaaaagc acacacaatg caccgattag
     1261 cccaagctct tctgtaataa tcgaaaaaat aaaatctgtt tctgtctctg gaagataccc
     1321 tttttttgtg atactatttc ctaatccccg cccaaatatg ccaccattat atagtgcata
     1381 aaacgaatgg gtcatttgat agcccgcacc atgactatcg ataaaagaat ctcttaaagt
     1441 cgcgatcctt tcataagcat gattaaacat ctgtggaagc caacctttat ttcctaaata
     1501 taataccccg cctgctgctc caatcagcaa tgcagaaaaa gtaacgatta gatagatccc
     1561 ttttttaaat ggaaccgccg ctgcccaaaa tatgacccac gcaatcgaaa ggatcatcaa
     1621 tgctccagca atctttggct gaaataaaat cagaaaagtt atacctactg taatcaggaa
     1681 tggttttttt agatcttttg ggctactatt tccgtcacga aaaaaccagc ttaaataaaa
     1741 aatcaaaaaa agatttgcca gttcagaagg ctggaattga atgccaaaca gagagatcca
     1801 acgttgtgcg ccgttgacag tgacaccgaa tatccctatt cttactaata ctaaaaagaa
     1861 aatcgataag gctaaaccgt atcctgctat tttagggtga agtaaatagt gtagtttgac
     1921 tgaacgagct aagaagatca cgctccaact caaaaatatg aaaattagtt gacgcaataa
     1981 tagacttttt gtattttcat cggcttgcaa taaacgataa gaactggcac tgtatacttc
     2041 taaaagaccg atcatggaca aagtcaaata aggacccaaa atccaccaat cgatcttttt
     2101 tctttttttc accgcatctc ctcctcacac agcaatccac ttgtcaggtg aggagcgagt
     2161 ttgtctcatc aaataagtca gtcacaaaaa tataaagaaa aggtcagaaa ccttttcgaa
     2221 caagaattct gacctagcat catctcatcg tccgagtttt agcactatac aacgtaaaga
     2281 aaacattctt agctattgga aaaagcctta atccgacagt ttctgtttta cccattggcg
     2341 actttcgacg tttctgggca acagcctatg tttgtatagg agcctcacct aacaaggtta
     2401 ttactatatt ctatttacga gttcatgata gacttttcct aatgattagg caatggaatt
     2461 tgaaatgaaa tagtgaaatt taacaatctt taatattttt tagaaaaaaa taacaaataa
     2521 gttgaaagaa tcccgttgca aattctttca gttgcaccag gattctttga taatttttta
     2581 gttttatgtg tttttttata tgtttttttc acttttcgga ataaatattt atttgttaaa
     2641 gtaattaaac aatccgctaa tcgtttgatc tggcatttct atttccttac catctaaagt
     2701 aacagtagta acgacaaaat ggtcttgcaa tggcggaact tctaagtact gcccttgcag
     2761 aaaatcattc actgtttcat attctttttt catttctttt gaatctgtcg atggtttata
     2821 gcttattttt aacataacta gtgctccttt ttattcggga cgtgcaatcg tgaaaatttc
     2881 tcctattgct gcataatcat ttcctgccaa acggcttaca gcattcaatt ttcttggatc
     2941 gatccttcct gtcttatcat catagactgt ttcatttaaa tgatatttct gtacttctag
     3001 caaaaataaa tcagctgtta ctttattttt attccgtatc tcgacatgtt catataagga
     3061 tgtttcaaaa cgaacttttg cttctgtcaa tgctggtact gaaatgatta ctgaatcagt
     3121 taaagtcaag tcagtcaggt acagttcgct ttcctcatta ggcagattgg ctgctgtttt
     3181 atttacttct tctacgttgt cctcatcaag gatatggata acagcttctt ttgtctcaag
     3241 aatattcttt gctgtgtctt ttactgcatc ttgttttctc tggatcgcca aggataagat
     3301 gggtcgattc gaactgacga tattgaagta actgaagggt gcgatattca ctatatcttt
     3361 tgctgattta gtcgttacta ctgctactgg tctagggatg actgaaccaa tcagcaactt
     3421 gtaattttca cgttctgtca attctgaagg actccgttca aacatggttt cgccttcttt
     3481 ctataaaatt cgttaaaaag cagatcttga agacaaaatg aaaagtaatg cttttttcat
     3541 cttcttagaa tctgctttaa actatctatc aatttttctt ttttgtcaag aatctgtgcc
     3601 gtgcgtgttt tttagttatt tgctttactt gcggcttcga catatctttt agtgatcaat
     3661 tgcggacggg tgataataga tccaactacg actgaaaaag cacctaattc atatgctttc
     3721 tctacttgtt caggagagtc agtatgtcct tccataatta ctgggatatc gacttgttct
     3781 atgatttgat tcaataaatc aaaatcaggt gtctgcgtct ttcgtgaata ttctgtatag
     3841 cctgctaaag tcgtagacaa aagatcaaaa ccatcttctt gcgcttgaag cgcttcctca
     3901 aaagtcgaaa tatctgccat agctaaccga ccgtgttcgt gaatcatttg gacaagttca
     3961 gcagttgttt tttctgtcgg acgtttacga gaagttgcat ccagtgcaat gatttctgct
     4021 tgcgtctcca aaagttcttc cacttctttt tctgtcgcag taataaacac ttctgaccca
     4081 tcgtaatctc ttttgataat ccctattacc ggtaaatcaa cgattcgctt gatttcattg
     4141 atatcttctg tcgtatttga tcgaatacca actgctccgc cttcttttgc agccaaagcc
     4201 attctcccca taataaaaga actgtgtagc ggctcgtttt ccaaagcttg acaagaaacg
     4261 acgactccgc ctttgatttg ttccagtgta tccacaatat ctcctccagt aatgttatat
     4321 aaaaatacag cttcatataa aaatgtaacc cttttctatt caggagtcaa taatgagcta
     4381 tttctttcag cagacctttc ttactaaaag aaaattactt caaattcttc gtatacgacc
     4441 agcttctctt ctgtaaattc tatgttcaac gcttctttat aagcatgttt aaaaaactca
     4501 gcatggactt tccgccaata agctaatgtc cgatcccctt ctccttcttt ccaagcatgc
     4561 tctttcgtga cttcactaaa aggcaagatg gtcacattta tatttttgat gatacaaact
     4621 ggttcatttt ttccattcag gaccacgtct aattgaccaa cttttggtaa aggctcattc
     4681 tcttgtttat acagatcgta accacttgat gtaccagtct ttatcttttt tacaactagt
     4741 tcgctcaact cgtctgccat tttttctgta tccccaaatg aaaagaaacc taatttttct
     4801 gattgataac taggatattt ttcttggaac tttttgacat attcttctac ttgctgatac
     4861 ataacatcca cccctttgct ttttataatg aaaaaaccat ccagatacta gtataaacaa
     4921 ttatctggat ggtgagcata atgaattatt gaatattcaa gacacgcaga ctatcttcaa
     4981 cgatttgttt tggtgttaaa tgataacgtt cataaagctg ccctacagga acgctatccg
     5041 tgaattcttt ctctgcacca aaatttaata cgttcattga agaatcacca taataacgac
     5101 tgatattttc gccaaatcct cctgacaaac taccatcttc taatgtgatg actaattcgt
     5161 gtgtttcttt taatttatct aatgtttcta cgtcattttc tgtgatcgtt cctggattga
     5221 tcaacgttgc ttcgattcct ttttttctaa aagcttttac tgtttctttt cctaattcaa
     5281 gaaaaccgcc taaagctaaa attgctactt ttcctccatt atttactgtt tgataggctg
     5341 gaatactata atcatttgcg atagctgtac catgttctac accatgtgta ggaattcgga
     5401 ttgctacagg ctgcgctgtt tgttcgaacg cccaatctag catagagatc atatcctctt
     5461 ttgttgctgg agcaagatat ttgatgttcg ggatacttga taagtaagga atgtcaaatg
     5521 ttccttggtg tgttcggtca ctagctgaga tcgttccgcc accgactaaa ataatcactg
     5581 gattctcgtt gatcgctaaa tcatgtgata attgatcata tgcccgttgc aagaaagtac
     5641 ttgattggaa gacgactgga cgtactccct gagaagccaa agcagaagcg aaagtgatag
     5701 agtgctgttc agcgatcccc acgtcaaaat aatgatctgg atgtttttct tggaatgttt
     5761 tcagtccaaa ggcgcctggt atcgctgcat tgactgcggc aataggagct gtacttgttt
     5821 gcattttctt atccaaataa tccatgatga tcatattaaa tgtttctcca gaagttactt
     5881 gctttgcctc cccggtggac agatcaaatg gtacatgcca atgatatttc attttattat
     5941 cgattgccgg ctgataaccg cgaccttttt ccgtatggac gtgaagtaca atcggatgat
     6001 caatatcttt tacttcttca aataaacgga tcaccgtttc cagatcgttt ccattatcca
     6061 cgtaacgata atcaaatccc attgctttaa ataggttatt gctgctttta ccgttggttt
     6121 gacgaagctc agctaagttt ttatataaac tgccatgatt ctcatcaata gacatctcat
     6181 tatcgtttac taatacgatt agattggatt gtaaaacacc accattgttc aatccttcaa
     6241 aagctaagcc accacttagg gagccgtcac cgataaatgc taccacattt cctgattctt
     6301 tcttcagatc tctcgcttta gctaaacctg ttgctaagct gatagatgta gatgtgtgcc
     6361 ccactgtaaa atagtcatgt ttactttcat gttggttgga atatccgctc acttcacggt
     6421 aatgtccttt ttcaaaacca ttttttcgac cagttaaaag cttatgacca taagactgat
     6481 gggaaacatc ccaaacgatt ttatcctttg gtgaatcaaa tacataatga aacgcaatcg
     6541 ttaattctac tacacctaga ttcggaccaa cgtgcccacc tacatcgctg tccttttgca
     6601 agattttttg gcgcatctct tctgccaact gtttcatttc ctcaacagat aattttttga
     6661 catctgcggg gctattgatt gcatccaata catgtaatac tgtactcata atacattctc
     6721 tcctccgtac tatcttcttt taatgaaaat agatttgtta cactttatct attctactac
     6781 ttagagttga ctctaagtca atagcatttt aaaaaaattt cagagaaagc ttgttatata
     6841 agtagatctt cgcacgaatc aggactcaaa taaaaacctt ctacagacaa ttaaaaagct
     6901 ttgtagaagg tctcctaatt aaggtctcat caagaaatat gtttttttaa taggtgaagt
     6961 tcctgagcaa tatgatacaa atggatgttt gcacctttta atgagattgg atagatcttt
     7021 gtcttttcag tttctaatcc attttgatag ctagcagcat cgcacaaaac cactcttttt
     7081 ttcgcacata gacattcatc aggcaattct tctaatgaac gaaataaaat aggatttatt
     7141 tcagcgatgc cttcttccgc atacaaatga aattgggttc gataaaaaaa ttgtttgact
     7201 gaactttctg aaacaacaaa aatttctatt tcctgtctcc ccgcttgttc actgttgatc
     7261 aaagaaagac ttttgacgaa ttttcgaatg aaattttcat tccatcttag ttggatttga
     7321 tttttctttt tccactcttc caatatttct acaatttttc cgtacagaag ttgttctttt
     7381 tctgatagaa gatagatcct ttccggtaaa aatacttgtc catctgccca caacgtacgg
     7441 acaaattgta aaaaggattg cttgaaaata aaattttctg catcgtcgat ttcagcgata
     7501 ctaataatct gtaaaatcaa ttcattcagt gataggtcat tgagaacaaa atttttataa
     7561 acaacttcga aatcttttct taacagctcc atgttctcat tggtataatt tctactgttg
     7621 aacaaagaaa aaatataata gacatcttct tcttgtaagt ctagaattcc tttttgctct
     7681 tccaatcctt gacagactaa ttgaaataac ggcttcgttt ctgcgctttt cttatcttct
     7741 tctttcagtc caaggggaaa tgcctttctt ccgattgcga tctctattcc tcgtatgata
     7801 aaggactttt catcttccga atatcttctt ttaaaaaaat gagtctctac ataatcaatc
     7861 aactcgttac aagcttcttc tacatacgga tttttatttt catagccttc ccaattgaca
     7921 taacgtacta aagctagcaa gatgttccgc gagtcaaatt ctgggataat gatttcttta
     7981 tttttcaaat aatcattttc tttaaaaaaa tccaatacga tttttttgat tgtatatact
     8041 ttgctcaaag aaataaattc aagatcagcc agttctgtac tcgtgaatat accattaaaa
     8101 taataactta aaaactttaa aaaataagat tgaccgtaca gaaactgtgt gagctcagaa
     8161 agagcaatac catgtttgaa tacgagttta tacttatgct tctctcttac ttgttctttt
     8221 atacaatatt ctagacactc ggctagactt tctagatcgt gaaggatcgt tggtgtagtc
     8281 acccttagag aaatagctgt ttgttcgatg tctatttctt ggaattcaaa taattgttca
     8341 cacagataca cttttctaaa tatatttcgc tcgatgtaat tttccagcat tcctgataca
     8401 cgcctccagt ttttcttatg attttattta atcatatctg taaatcgtat ttttgtttcc
     8461 tttttattta gaaaagccct ttcaaaaacc aataaaaaaa gaaactggga caaaagtcaa
     8521 acaagctcac aatttcagcc tcattgtagg tagctttgag ccgtttggac ttttacccca
     8581 gcctctttca agaatggtta ttcaaagatg tcagcgtcta gttctttgac tacatctttc
     8641 aacaattgga taccagctgc gctgttacca aatggatcta gtgccgggct gaagacacct
     8701 atgccgtaac gatttggcac agctgccatc aacccgccgc ctacaccgct ttttgcaggt
     8761 acaccaacat ggacggaaaa ctctcctgat tcatcataca aaccagcagt tgtcataatt
     8821 gatttgacga ttgtagcact ttcttcagta atgatttgtt tgccattcca aggagcaatt
     8881 cctttatttg ccagcacagc agctaatttt gctatgcctg tagcggtgac attgagggag
     8941 cattgtctga aataagcatc gagtatctcc ggtacgtctc cttcgatcat ttgattacct
     9001 ttcatatagt aagcaagaga gcgattgata tcacctgttc ttgattcaga atgatagatt
     9061 tcttcattca atgtgatatt aggatcatca cagatctctt tcataaatga caagattcgc
     9121 tcaaattttt catctgcatc tttaccggca atcaacgagg ttgttgcaat cgctcctgca
     9181 ttaacaaaag gattcattgg atgtttgcga tgattgattt ccatattcat gatagaattg
     9241 aaggcaaaac cggttggttc cgtgttgatt gtttgaaaga ctttttctat tccgttgtct
     9301 ttgattgcta ataacaatac gggaactttt gacatgcttt cgatggcaaa gcgcacatcg
     9361 gcgtctcctc cctcaatcag ttgttttgta tccaaatcat aaatcgccat acctaattgt
     9421 ttgggatcca cttcagctaa tgctggaatg taatccgcca cttgtcctgt ttgataatag
     9481 tggaaatttt ttccaatcaa ttgttctaat tcttttactt ccatttgaat aaccagcttt
     9541 cattttttat ttttctgcat gctggaattt atgacggcat gcgtagatcg taaatggaat
     9601 aactaagacg ataagataag aaacaatcag aatcgtttca taaatcccat gctgtccagc
     9661 tgcgatacta gcaggtggaa caaatgagat acaaaaagca aaaagtgaag tcaaaagacc
     9721 taaacaagca acgattactt ttacaacttt tccgcctgga acctgatagg ctctttcaag
     9781 actgtcatct ttaaaaacca gcttcaaata gcccgcgaaa aacataaagt acaacatcag
     9841 gtagattaca acagtcaatg acatcgctgc aagaaatgaa agattattcc ccccgccgct
     9901 taatgtaaag acggctgccc agattgttgc aacgatccca tttgcgatca tcaaatgagc
     9961 aggaacaccg tgtgtgtttt cttttgcaaa atattcagga agtaatcctt cttctgctgc
    10021 tacgtgaagt gcttttgaag gagaggttac ccaagaagca atttctccga tgacacctaa
    10081 acaaacaaga atcgcaagaa ttttcgcaat ccatgcaaga tttggatcaa tgtgtcttaa
    10141 taggaattct aacgattgaa tcacccctgt attaagagac aaaccatgtt gaggtactgt
    10201 agtagcgacg gtcaatccgc caaaagtatc taaaatgatt gccagaataa ccaatacaaa
    10261 cattgctaat ggataatcct ttttagggtt ttccatttca tttgcatgac tggcagatgt
    10321 ttctacgccc atatatgcta aaataaagga aacaaaaaca accagcgtag gcaatttcga
    10381 aaaatcagga atcaatgttt tcatagacag tatcgtttgt agtggagcgc ctgaagcgac
    10441 ataatggatc cctaagccaa acaagactaa tgctggcaac aagataccag ctacaaaacc
    10501 gatccgagca atcaatgaag tgtatttggt tccttttaac tgtgagagta aaatagccca
    10561 gaaaataatc aataggattg ccaatttcag atatttattg ttttgtatag caccaatacc
    10621 cgttgcatag gataatgcac cagttaagaa atacagcatg gtcaagtagc caactgttat
    10681 ctcaaaccat tggaaaaaga ttgcggcaaa accgaatcgt tttcccaatg tgcgactgac
    10741 ccaagtatag acaccgcctt tttcccatcc tttcaccgtt gccatctctg ctgcgcacaa
    10801 tgccacaggg ataaaccata gcaaaccgcc taataacaag aaaaatacta agctgaagcc
    10861 tgaggtcgcg aatgtgggat attcatataa tgaaacgacc attgagatgg tcatagccaa
    10921 aaagccaaag aacgttaatt tttttgcagg aattttcgtt ttgctttgtt gcatgaatct
    10981 tcctcttttc tatttgaatt tgtttcaatt gagtttgttg ccaaagaaga aaaagtccaa
    11041 tccttcttct tgttcttctt ttgtagtagt gatcgttgta aagtgcatcg ccgattttaa
    11101 aatcactgtt gtcagcacat aggcatacac ggcagtaatg atgacaccaa tcacttgaat
    11161 ccctaagaag taccagctgc ttggattcgt tagtaaatgg ctgttagatg caaagatccc
    11221 tatcagaatc gtacctgtga atccgcccat gccatgaaca ccccacacat caagtgcatc
    11281 atcccattgt ttctttttac gaaaatcaac agcagcatga cagacgatac ccgcaatgat
    11341 cccaatcagc atggcacttt ttgcttctac ataaccggca caaggcgtaa tggctgccaa
    11401 tccagcaacg gatcctgtta gtacgtcgac aaaatcaaag ccatttcctt ttacttttgc
    11461 aatgatcagc caagtaatca tcgcaaatgc aagagcgatg aatgttgaac caaatgcatt
    11521 gactgcttgt tcatctgctc gaagtgcacc accggaatta aaaccgaacc agccaaacca
    11581 aagcagtcct gtaccgatag cagctgccat aagactgcta tgctgacttc tttctcctaa
    11641 aggaatcttt ctttgaccaa gtgtgaggat acatgctaaa gagccgaatc cagctgtcgt
    11701 gtggatgact gctccaccag cgaaatctct aaatcccatc tgatccaaaa atccgccacc
    11761 ccatatccag tggcaaacag ggaaataaat cagcagattc caaaaaataa caagtataat
    11821 atagcctttt aaattcaatc tccctgcaaa agctcctgtc atcaaaggaa cggtaatcac
    11881 gataaacatc agttggtaaa gaaagaacat taaaaatgga atcgttgcac catgaaacat
    11941 gttggggaag cttgtaacat gtctcatcaa aaagaaatct gccgggtttc caataacacc
    12001 gccgatatcc cgtccaaagg ccaatccgaa tccaccaaat acccacataa tcgttgttat
    12061 cccaacggag agaaaagatt gagacatgat cgttagtata ttattcttcc gtgctaatcc
    12121 gccgtaaaaa aaagctagac caggagtcat caggcaaacc attgctgtac ataaaatcat
    12181 gaatgctgta tctcctgtat tgataggtaa tgtattcatt tttttcgcct cctttctacg
    12241 attacgagta taaaagaagg tgtagttgtt tttttcttct gtttttttga attaatactt
    12301 gcatttataa aagacagaca attaataaaa gcttgatgtg ttgtgctctt tttcgttttt
    12361 cacacaaata aagacaaaaa ataaacggtc ttctttttat agaaaaccgt ttattttcgt
    12421 ctttttttat ttttaaagat atttt
//
LOCUS       DARBEC010000081        12198 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.084, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000081 DARBEC010000000
VERSION     DARBEC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12198
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.084"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(160..324)
                     /locus_tag="SCL80_002195"
     CDS             complement(160..324)
                     /locus_tag="SCL80_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108042.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            619..1947
                     /locus_tag="SCL80_002196"
     CDS             619..1947
                     /locus_tag="SCL80_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEG1108043.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            2149..2982
                     /locus_tag="SCL80_002197"
     CDS             2149..2982
                     /locus_tag="SCL80_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEG1108044.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            2998..3735
                     /locus_tag="SCL80_002198"
     CDS             2998..3735
                     /locus_tag="SCL80_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEG1108045.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(3890..4858)
                     /locus_tag="SCL80_002199"
     CDS             complement(3890..4858)
                     /locus_tag="SCL80_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEG1108046.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            5090..5923
                     /locus_tag="SCL80_002200"
     CDS             5090..5923
                     /locus_tag="SCL80_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEG1108047.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            5957..6796
                     /locus_tag="SCL80_002201"
     CDS             5957..6796
                     /locus_tag="SCL80_002201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1108048.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            6822..7382
                     /locus_tag="SCL80_002202"
     CDS             6822..7382
                     /locus_tag="SCL80_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1108049.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            7581..9302
                     /locus_tag="SCL80_002203"
     CDS             7581..9302
                     /locus_tag="SCL80_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEG1108050.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            9467..10609
                     /locus_tag="SCL80_002204"
     CDS             9467..10609
                     /locus_tag="SCL80_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEG1108051.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            10625..11836
                     /gene="thiI"
                     /locus_tag="SCL80_002205"
     CDS             10625..11836
                     /gene="thiI"
                     /locus_tag="SCL80_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEG1108052.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
BASE COUNT         4154 a         2048 c         2535 g         3461 t
ORIGIN      
        1 ggttcgagtc ctactgccgg agtaaataaa gcttatgcaa agaaaaagaa atccaatcac
       61 ttgggtttct ttttctttta ttattatgat gaatcaagct ttctgttttt gtcacgtatg
      121 ttgtaatctt tacgaaagag tagagttggc tactcataat caaccatact tcccatcatc
      181 gctaaatacg aaataaatca gcattcctac accaatcaaa gcacaagctg caccaagaaa
      241 agggatgaca ttcccaaagt tatctgtatt cataagtatg ccaccaccca caaacaaaaa
      301 gccgccagca atcaggtttt tcattttatt catctccttt cttacgttat agtatttcct
      361 tctttcaatg agtattttga aagctatctt attgtatgat gttcttctag gcctcttctt
      421 tattatacag gccattttcg ttatctcata gaaatattcc tttttatctg aaaactattg
      481 tataccgtat gacatttttc ttcatgaagt tgtaagtata ttctcattca aatgaaaatt
      541 tcatcataga agagattgta tttctaaatt cttaagagta aaatacgaag tataacttgg
      601 atttggaagg atgattggat gaaagctatt acgcctgata tgacaacaaa atttaacaaa
      661 cagtttcaag caaatatcaa acagcaggca ttgcagcggg cagttgtgaa gaacggaatt
      721 actgcttctg ctgaaaatgt ttcagcacat gtggagaata caccggtatt ttctatagat
      781 ttggctacgg ggaaagtggc taatcaaatg caatcggggc gttgctggat gtttgctgca
      841 ctgaacacat tccgtcataa gatgctgaat caattccatt taaaagagtt tgaattatca
      901 caaaattaca cttttttctg ggataaatat gaaaaggcaa attatttcta tgaaaacatc
      961 attgcaacag gaaaagaggc aatcgatagc cggaaagtag ctttcttact cgctactcct
     1021 caacaagatg gcggccaatg ggatatgatc gtttcattat tccaaaaata tggcgttgtg
     1081 cctaaatcgg tgatgccgga aagtgcgaat agttcaaatt ctcgagattt gaataactac
     1141 ttgaacaaat tactgcgtaa agatgcagta gtgttacgga aaatggttgc tcaaggagaa
     1201 acattagacg ctattgaaga aaagaaagaa gaaatgctag aaagcattta taacttctta
     1261 gctatctcat taggaacacc tccaaaagaa ttcgattttg agtaccgcga tgaagaaaag
     1321 aactatcatc ttgaccgagg actgacacca caaatttttt atgataaata catcggtgta
     1381 aaattagatg attatgttag cgtgatcaat gcaccaacaa aagacaaacc attcaatcgt
     1441 tcttatacag tagagatgct aggaaatgtc gttggcggga aagaagtcaa atatctgaat
     1501 gttgatatgg aaactttcaa aaaattagcg attgctcagc ttgaagaagg agaatctgtt
     1561 tggtttggtt gtgatgtagg tcaatcttct actagaacta gtggcatcat ggccttagat
     1621 gcttattcaa tggatgatct atttgatacg gattttacaa tgactaaagc tgaacgttta
     1681 gattttggag aaagtctgat gacccatgcg atggtattga ccggtgttga cttagtagac
     1741 gggcaatcaa caaaatggaa agtcgaaaat agctggggcg aaaaagttgg aaagaatggc
     1801 ttctttgtta tgagtgatgc ctggatggac gaatatacgt accaaatcgt tgtaagaaaa
     1861 gatttattga cgaaagagca attaaatgcg ttcaatgaag aaccaattgt tctatctccg
     1921 tgggatccaa tgggtgcttt agcatagacg agtatttcaa tgaagagtat gtagtaaaga
     1981 taaaaagagc tggcttaagc cagctctttt tatctttaaa gtccattccc tgttttttat
     2041 ggtccctgtg ctataattct aaataaagga taaggattgg aaataatcct tttatcatta
     2101 aaaatatgat gacgaagtag aataaaattg ggagtagggg ggaaagtcat ggatatccaa
     2161 actgttttat ctgatctgaa attaaatggt aaagtgattc cagttgttgg tggcgatgtt
     2221 aatcagacct atcgtataaa aaccgaacat cgtgcttatt ttttaaaaat ccatccgaat
     2281 gtaaaaaaag gattttttga agcagaagta gacggattaa aagaactgtc agcattcgta
     2341 cgtgtaccgg atacgtatat gctaggtgaa acaagtgaag gcgcttattt gttaatggaa
     2401 tggatcgagc cgggaaaagg ggatcaacga gatcttgctg cagctttagc taatctccat
     2461 cagcaaacag cgccacaatt tggttttcgt aaagataact atttgggtac acttgttcag
     2521 aaaaattctt ttgaagaaga ttggtggact tttttcttta aagatcggct tgaatcccag
     2581 atttctttgg cagaagaaac gaatcgatgg aatgtacagc gtcaagaaaa gtatctgcga
     2641 tttaaagaac gtgtattgaa atcggttgaa ccaaaaaaga tcactccacg cctcttgcac
     2701 ggagacctgt ggagtggtaa tgtgttcttt gaccagcaag ggcatccagt ttttgtcgac
     2761 ccagctgttt cttatgggaa tcgggaacaa gatattgcaa tgagccagtt attcggtggt
     2821 ttccgaccgg aatttttaga tgcttatcaa actatatttc cattagaaaa gggatggaaa
     2881 gaccgactgc cgatttacca gttatattat cttttggcac atttgaatat gtttggagaa
     2941 agttacggtt cccaagtaga tcaattacta gaaaattttt aagaggaggg cgtgtttttg
     3001 gcaaaaaaat ataccaaaaa gcatgaagtt tcatattatg agtgtgacat caaccaaacg
     3061 atgacatttc cttcgatgtt aggagtagtt atcaaaacgt ctgaagatca aagcgaccag
     3121 ttgggaagag gcagtgaatt cgtcaatagc tttggactta cttgggtcat cacgaattat
     3181 tccatggaga ttaatcgtct ccctagagtc ggtgaagtaa tctcagtaac gactcaagcc
     3241 atggagtaca ataagttttt ctgttatcgg aacttctgga tccatgatga agatgggaaa
     3301 gaattagtca aaatagaatc tgttttcgta ttgatggatt ttgtgaatcg aaaaatgagc
     3361 agtgttaatg aagagatcat tgctcctttt gagagtgaga agatcaagaa aatcaagcgt
     3421 caggaaaaaa ttgaaaagat tgattcgggc ttcatgttgc catatcgtgt tcgtttttat
     3481 gatatcgata gtaatcagca tgttaataat gcgatgtatt ttaattggat cattgacgta
     3541 ttaggatatg actttttgac tacgcatgtt ccagaaaaag taattattcg atttgataaa
     3601 gaagtagagt acgggaatga gatcgaaagc cattttgaac aaatccctat cgaaaatggt
     3661 gtcaaaacaa aacatgaaat ccgaatgcaa gaacagctgt attgtgaagc aaatattata
     3721 tggaaaaata aataaaaaag aggttgtgac agaagggggc aacttcaaga aataagaaga
     3781 aatttacgaa aattgtttct caaatttttg taaattttcg acttagtttt cgaagaagct
     3841 atttttgtca cagcctctct ttttaagttt agctattaga taatgatgtt tagttttgga
     3901 taaacgaaat caattcttca cccgacaaac catagtttac agcaatcaat agttttaaac
     3961 gtgctttttg actattaata ctgttacaga aaataatccc cattttttct agttcttttc
     4021 ccccgccttc ataatcataa acaggctctg cgattccatt gaaacaacga gaaacaagta
     4081 ctacagggat tttttggaca agaagctttt ccaaagcagc taaggcttgt ggagggagat
     4141 tccccgcacc taaagcttcg attaccaaac cgtctacttt cgtatgggcg atggcttcca
     4201 ataagtcccc ttgcatccca gcatatgctt taacgatcgg gatagtccca tctacagcgg
     4261 aaatgtccag tcgctttgtt tctaaaagtt cctgcaagaa taaaattcga ttcttagtta
     4321 ctaagccgat gggacccaat gtaggtgttc ggaaagtagc aacatttgtg gtatgggtct
     4381 ttgtcacgta gcgtgcagaa tggatctcat cgttcatgac caccaaaacc cccttatcta
     4441 gcgcttcttc acaacttgcg acacgaatcg cactttcaaa attataaagg ccgtcacttc
     4501 caagttcatt acttgaacgc attgccccag tcaaaacaat tggtaatttg ttgccaatcg
     4561 ttgtatcaag aaaaaaagca gtttcttcta atgtatctgt accgtgtgtg atcaccactc
     4621 cggatatacc tgatgcataa gcggacaaga tacgttcttt taataccaac atgtgagaag
     4681 gcatgatgtg tggtgaggga agattgaaga tatcttcaac taataattct acatgcttgg
     4741 ggattgtgat ttctgcatct aataatgggt ttgccacatc aggactaaca ccgcccgtga
     4801 gcatgttttc tttcatggcg atcgttccgc cggtgtgtag tacaagtatt tttttcattt
     4861 tacagacttc cttacgaata attttgcata gccgttaaat cttattgtat actatataaa
     4921 agagttttgc tatcagtgaa gaataacgaa tagcctaact tatcataaaa aagctgacct
     4981 tattaaaatg gaaaagactc aaccttgacc tcctaataca atggagtatc tggttgagtt
     5041 tcttttacaa gaaagcagcc aaggaaatca atcacgaagg tggccggaaa tgatcagaat
     5101 cggattaaca tcgtttaaag aacatgacaa attaactggt aaaaagaata acactcttta
     5161 tgaatatgca gggtttctac ccttagtgga attagatact gcctattatg gaattccccg
     5221 aaagtcctca gttgagaatt ggataagtga agtacctgaa tcatttcgct ttgtagtcaa
     5281 agtctatcaa ggaatcagcg gtcaaggaga ctggaagcct tattatcaga ccgaagaaga
     5341 gatgattgcg cattttttag atgttatgtc gccaatggta gatgctggaa agatgttttg
     5401 ctttttagtc caattcccct catcattcaa atgtacgaaa gaaaatgtcc agtacttgcg
     5461 caaacttaga aagtggtttg agggttatcc tgtttctata gaattgcggg attattcttg
     5521 gtacagcgat gaatttcttg aacgtacaag aaactttatg tctagagccg ctttttcttt
     5581 agccatcatc gatgagccac agttgctgaa tacaacgatt ccttttgatc catttgtcac
     5641 caatccgaac tttgctcttt ttcgattcca tggtaggaat gcgcaaggat ggcaagcaaa
     5701 tgataaagag tggcgtaaaa aaagaacgtt atatcgttat tcagaaaaag aactgcaaga
     5761 actagcacat gaagtcaaag tagttgattc taagacaaaa gaggtaggtg tgatttttaa
     5821 taataattct ggtggtgatg cggctgaaaa tgcattagca ctgcaaaaaa tattaaatat
     5881 caagccagat ggactcaatc caacccaaat ggatctgttt taagggctta ttttattcaa
     5941 gaaaggggcg aagtctatga tcaaagctat tttttttgat attgacggaa cattggtcac
     6001 caatcgttca aaagcgttgg aatcaacaaa acaagcaatt gaatatgctc ggaaaaacgg
     6061 cattttatgc ggtgtagcaa cggggcgcag cccagtaaag atcaaagaaa tcattgatga
     6121 attggaatta gatatgtatg tcgtgtataa tggtcaactg gtttttactg cagatcgcac
     6181 gattatcgac cacccttttg aacaaaaagt attggaacat attgttgaat ttgcagatga
     6241 gaaccatcga caaatcgttt ttggtgcgag aaatcggtta gatggatcaa cgacgatgct
     6301 attaggacaa tcgattttta tcaagcgact agtcagcttt ttaccgcgta aattcccggt
     6361 acgtctgatg aaaaaaatcc tccaggtttt tagccctcac cgacaaaagg atcgttatga
     6421 aacgttagag atattaaaag aaccaattta tcaatgtata ttgctcagtc cggagtcaga
     6481 acagaaaaag ttagaacatc gctttcccga gtgtacgttt cagcgttcaa atacgtatac
     6541 tgtagatatc atcccaaaag gtggttcaaa attactaggc atccaagctt tcgcaaatgc
     6601 tgtcgaaatt gaaatggaag aaattatggc attcggcgat cattacaatg atatcgagat
     6661 gctgaaaggt gtaggaattg gagtggccat ggggaatgct cagatcgaag taaaacatgc
     6721 tgcagacttt gtgactcaat caaatgaaga agatgggata tacgaaggac tgaaacactt
     6781 cggtctgatt tattagtcaa tgaaagaagg aaaaaatcag tatggaagca gagacacctt
     6841 ttcaaaaagt agaaacattc cacaaaactt ttgatccaag aagaccagag atacccacgg
     6901 cttttacaga aaatgaagct catacacgtg ctgcgttcaa aatagaagaa cttgttgaat
     6961 ttgttgcagc tacttcacct aataaagaac aactgcatga agctgttatg ctgctgcacg
     7021 aagcattaga taaagcagaa gaaaaaatac ttggtaaaac acagtgggga agtgatccgc
     7081 tggttgaaca agtagatgca ctgacagata tcctttactt tacttatggt tctttttcat
     7141 tactaggaat agtgcctgat cgaatattcg aaatcgtcca tcaggcaaat atggggaaat
     7201 tatttccaga tggaaaacct cattatgacc ctgtaacaaa taaagtgttg aaacctgaaa
     7261 actggcagat ggaatatgct ccagaaccta aaatcgcggc agaaatccaa cgtcagattc
     7321 aagaagcgat tgctaagaag aaatccgctg atataggcat gtgcacaaat gaaaaagagt
     7381 gatcagagtt tcactatttt tgttatcttt atacgatgga aaaacaaaag attttcgttt
     7441 aagttagtga atgcatgaaa atagtatgct ataatctaga atagatgtct tttgcacgag
     7501 agacgtatta tttggattta tgccaagcag aacgccatct gttgcataaa agatgcatac
     7561 tgaaaacgga ggaaccataa atgggaaata atgttatcat cggaatcgtc attgcaatca
     7621 ttatagtcgc cattgtcgtc tttttggtta gttacttgat gaagaaaaaa aaccaagatc
     7681 gattggatgc cttagaaaaa cgcaaggaag aattatttga cttgccagtg attgaagaag
     7741 tcgatgatgt taaaaaaatg catctggttg ggcaaagcca aaataccttt cgtgaatgga
     7801 gtcaaaaatg gaatgacata tctacttcct cgttcgctga actagaaagt caaattttcg
     7861 aagtggaaaa cctcaatgaa agttttcggt tcttaaaagg aaaaaaagca gttgttgaag
     7921 cagaagctac aatggaccaa atggagaaac aagtgacgga aatccgtgaa ggtctaaagg
     7981 aattgcggga aacggaagaa cgcaactcac tagctgttca gcaggcactt gacgtctatg
     8041 aagaactgaa agaaaaagtc agagaagacg agcatgaata cggaccagct ctttttgaat
     8101 tgcaaaaaca gatcaaagtg atcgaaaaag aatttacgca gtttgtgact ttaaacactt
     8161 ctggagaccc tgttgaagcc cgcgaggttt tggataaagc agaaaaacat acgtatgaag
     8221 tggaagctct gatgaaaaaa attccaccgc tgtatgagga gctccatacc actttccctg
     8281 aacaacttga agaaatcagt gatacttacg agaaattgat ggaagaacaa tatgtattcc
     8341 cagaagaaga tcttgctgaa gatatcgcaa aagtaagtcg aagaattgaa aattcattag
     8401 ccaatcttga aaaaacagaa gttgaaacgg tcgaatttga aaaccgtgaa acagctgatt
     8461 tgattgattc gttatatgat atcttagaac gggaaatgga agctcagaga tatgtaaaaa
     8521 ctaaccaatc aacgattgca gagtatatca aacatacaac aaagaataac cgtcaattat
     8581 tgattgagct ggatcacaca tctcaaagct atacattgaa ccacaatgaa attggccgtg
     8641 tacgaggttt ccaaaccgaa gtggaagaaa tggaacgtca aaatgaacag atgattccac
     8701 agatccgtca acacgaaatt ccttattcag aagtgcgcac gttctataaa acagtcttca
     8761 aagtcttaga agatatcgaa aagcaacaag tagagatcga cgactcatta catgaattga
     8821 gaaaaggcga aaaagaagcg caagaaaaga tcgatacatt cgaattcaaa ttgcgcagtt
     8881 tgaaacgttt tgtagaaaaa caacgtttgc ctggcttgcc gaatgattac ctagaattct
     8941 tttttgttgc tactgaccgt attgaagaac taagtgttgt cttgaataaa attcgagtaa
     9001 acatggaaga ggtcaatcgt ttagttgctc tatgtgaaga agatctagaa ttattagata
     9061 agaagacaca tgatttagta gatgcagcag cattaacgga gcaaatgctc caatatgcaa
     9121 atcgttatcg tcatacacac gaagaagtga gagaagcaat tgataaaagc tactatttat
     9181 tcaataaaga atatcactat caagaagcat tagacgaaat cggtacagca ttagagcgag
     9241 tagaacctgg tgctttcaaa cgcatcgaag atttttattt taatcatcca gacttagtat
     9301 aaagcaaaaa agttagtaaa ccagcaagag caagacggat tattgtcttg ttcttgtctt
     9361 tttttgtata tttgaaaatt gaaacaagtg aaaatttcac ctgctcttga aagcaccttg
     9421 gacaaccgtt ataatagaaa agaatttcaa aaaagagggg tttcttatga tatattttga
     9481 taacagtgca acaaccccaa tctttccgca aagcttagat gcatatgtaa aaaccagtca
     9541 gcggattatt gggaatccat caagcttgca tgatttaggc aatcaagcta accggttgtt
     9601 gcagcaagca agaaagcaga tcgctgattt gatccatgta gaaccagaag aaatctattt
     9661 tacttcaggc ggaacagaag gagataactg ggtattaaaa ggaacgatga tcgaaaaaag
     9721 agaatatggg aatcatatga tcatttcagg agtcgaacat ccggccgtca gcgagacagc
     9781 agaacaatta aaagaattag gaatcgaagt aagtatcgcc ccagtggata aacgcggatt
     9841 tgtacgtgtc gacgaattga aagaattaat aagaaaagag acagtattag tctctgttat
     9901 ggctgtgaat aatgaagtag gatcgattca gcccatccag gaaatcagtg atcttttgga
     9961 tgattttcct aaaattcatt tccatgtaga tgctgttcag gcgattggaa aagtaccatt
    10021 ctcgcaatgg ttgacagaaa gagttgattt tgcaacattt tcagctcata aattccatgg
    10081 cccacgggga actggtttca tttattggaa aaagggtcgt cgtttagcac cattactgac
    10141 aggtggaggg caagaaagaa atcagcgaag tgggacagaa aatgttccag gtattgtggc
    10201 tatggttaaa gctttacgtt tatcattaga gataaaggaa caaaaacctg agcagacaca
    10261 acgcttaaaa gtagctctaa tcaatgcgtt gaagacgtat gagaaagtga cgattttttc
    10321 agaaggaact gactatgcac cgcatatttt atgttttgct ctaaaaggaa ttcgcggcga
    10381 agttctcgtc catgccttag aagaaaaaca aatatttgtt tctactacta gtgcctgctc
    10441 ctcacgtaaa catatggcaa gcagtacatt gcacgcaatg catgtaccaa atgatctggc
    10501 tacatctgct gttcgaatca gcttagatga aaacaattcg cttgcagaag tcgagcaatt
    10561 tatgattatt ttcaagcagc tttataagaa attttctgca atcaattaaa agaggagtgt
    10621 tcttgtgaaa tacagtgaaa ttatggtccg ttatggggaa ctttcgacaa aaggaaagaa
    10681 tcgaaaaagc tttatcatgc aactagcaca aaatgtcaga caagctttga aagactttcc
    10741 agaaatcaaa atccatgctg atcgggaccg gatgcatctt ttattgaatg gcgcagacgg
    10801 gcagctagtt attccaaaac tagcaaaaat atttgggatc caaaatttct ctccaagtat
    10861 tcgtgtagaa aaagatgtcg aagttttgaa acaagctgtt caagacatca tgaaagaaat
    10921 ttatactggg aaagaaacat ttaaaattat cgctaaaaga agcgatcacc aatttgaatt
    10981 gacaagtaat gaattgaatc aaacattagg taatgctgtt tttgatattt tcccgcatat
    11041 ccaagttcaa atgcgtcaac cagatattcc attacgtgta gagattcggc gtgatggcgc
    11101 ttatctatcg tatgaaacga tcaagggagc tggaggattg cccgtaggaa caagcggaaa
    11161 aggaatgctt atgctttctg gggggattga ttctcctgta gcagggtact ttgccatgaa
    11221 gcggggagtg gaaattgaag ctgttcattt tgctagtccg ccgtatacaa gtgaacaagc
    11281 actgcaaaaa gcaaaagatc taacagccaa actagcacct tatgtgggaa cgatccattt
    11341 tatcgaagtg ccatttaccg agattcaaga agaaatcaaa aagtcagtgc cacaaggata
    11401 ttggatgaca attactcgtc gaatgatgtt gcgtttgacc gatgaaattc gccgtattcg
    11461 tcatggttta gtcattttga atggtgaatc attaggtcaa gtggcttcac aaactttgca
    11521 aagtatggtg gctatcaatg aagtaacgaa tacgccaatc attcggcctg tagcgacaat
    11581 ggataaactt gaaatcattg aagtagcaga acagatcgat acatttaatc tttctatcca
    11641 gccttttgaa gattgctgta cgatttttgc tcctccacag ccaaaaacac gccctcaact
    11701 ggaaaaggtc catcaatatg aagagcgttt agcaatagat gaaatgatcc aacgagcact
    11761 agctggcttg aagatggaag agatacaagc agaatcgtct aatgaaaaaa ctgaagaatt
    11821 ttcagaattg ctataagagg ttatgaaaat agtgttttga ccagagtatt agagaaacaa
    11881 aacgaaaaaa tagctccatg tattttaccg tttttgaact aatgtcgaag attagctgat
    11941 tgaataccat ttatcaagaa gttatggaaa agccggttct tttttactaa ctgaaaaata
    12001 aaattttatt tagagatcaa gctacagttg ataaatgata taaaaaagag aatttcccat
    12061 gaaatagcga ggttgtgaca gaagtgggca gcttcaagaa ataagaagga atttacgaaa
    12121 attgcttttc aaatttttgt gaaatttcgg cttatttttc gaagaagtta cttctggaac
    12181 accgtgtatc agaggttg
//
LOCUS       DARBEC010000082        11669 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.085, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000082 DARBEC010000000
VERSION     DARBEC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11669)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11669)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11669
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.085"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..217)
                     /locus_tag="SCL80_002206"
     CDS             complement(38..217)
                     /locus_tag="SCL80_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108053.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESRIMCIALDLL
                     IAQEKAGFEVPALKK"
     gene            complement(598..1305)
                     /locus_tag="SCL80_002207"
     CDS             complement(598..1305)
                     /locus_tag="SCL80_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108054.1"
                     /translation="MNKVVYQLTTEDVSQFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYMESETSQTKVTVSHNRLDVYKRRPDTLKKGAELLRLETEKYIMNVYLERS
                     ELVENFVSYTICRKHYYSESEYYSIFESMELMEEEMEELNSKDATVDDYYLVFTRFLT
                     CGTESIVLMGMDYELTVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKGQ"
     gene            complement(1298..1735)
                     /locus_tag="SCL80_002208"
     CDS             complement(1298..1735)
                     /locus_tag="SCL80_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108055.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKEKLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNGKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYAKFAQIKKAKAMASDWLDTFENIEKNGAFYTIESEEKDNE"
     gene            complement(1750..2001)
                     /locus_tag="SCL80_002209"
     CDS             complement(1750..2001)
                     /locus_tag="SCL80_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108056.1"
                     /translation="MKTQHKLLTVKEVVQILVDKEVISEKVAEALLIPRYWTIETPLV
                     EDAPTMLFEDLVKIAGYELHYQENMETYEIEYWLTKLEN"
     gene            complement(2001..2207)
                     /locus_tag="SCL80_002210"
     CDS             complement(2001..2207)
                     /locus_tag="SCL80_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEG1108057.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGERAKCIKSYRLYQC
                     PTCGKKYTLSYGDYVLIDEKVKKR"
     gene            complement(2644..2904)
                     /locus_tag="SCL80_002211"
     CDS             complement(2644..2904)
                     /locus_tag="SCL80_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108058.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIESMQIRNPDHFTRSMGI"
     gene            2979..3182
                     /locus_tag="SCL80_002212"
     CDS             2979..3182
                     /locus_tag="SCL80_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative holin-like toxin"
                     /protein_id="HEG1108059.1"
                     /translation="MIKTVKFVTVAIRLKVRWCVCAYFQNLTERRSLLPVETALGLMI
                     SFGSLIATIIFGILAVVNNNQKK"
     gene            complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="SCL80_002213"
     CDS             complement(3323..3808)
                     /gene="ssb"
                     /locus_tag="SCL80_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1108060.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMATLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSSSPSPTTFEQRDVATPNNNGLNASQNPFGGQSIDISDDDLP
                     F"
     gene            complement(3811..4710)
                     /locus_tag="SCL80_002214"
     CDS             complement(3811..4710)
                     /locus_tag="SCL80_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEG1108061.1"
                     /translation="MTPTQFAAKTRNLAKSTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTEQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIRGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            complement(4723..5316)
                     /locus_tag="SCL80_002215"
     CDS             complement(4723..5316)
                     /locus_tag="SCL80_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEG1108062.1"
                     /translation="MQDKLKELLAIRDGNLSMKEARKFGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEFEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            complement(5392..5541)
                     /locus_tag="SCL80_002216"
     CDS             complement(5392..5541)
                     /locus_tag="SCL80_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108063.1"
                     /translation="MRNTTHKQQEIENQKAKIRLEQEKLRANKRRLQQLKNQQRIQKE
                     IELDR"
     gene            complement(5557..7761)
                     /locus_tag="SCL80_002217"
     CDS             complement(5557..7761)
                     /locus_tag="SCL80_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 3"
                     /protein_id="HEG1108064.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFSGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQAGADHGKIFKRLWINSLEKEAIYQGFQQLRDAEETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FQKEPYFEGKAQVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGTIHQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLKAEAIPTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTAAAFEQ
                     LSPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKTVGKVPVDLGWKKLFGK
                     ESDRKEKEEEPLLPKVTKGEAVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGILLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGEQKGTQATFLANIQKFVSHLLEVVPGQIQSTDFGSTLQEVKA
                     ASEKQEATRHLGVCPKCREQEVLLYQNVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNGRTSQSVRGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNYKKRTRRTTK"
     gene            complement(7772..9232)
                     /locus_tag="SCL80_002218"
     CDS             complement(7772..9232)
                     /locus_tag="SCL80_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEG1108065.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKHERQNF
                     LVRDDRLDHAVAFLWKDPQTNETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQLKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKVI
                     YEEVAKEEKITPEAIMAIHKTENNLQLTNQLVSAHKVNASFGQQLSVNDSIEAINLKD
                     ICREVAKELKACERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIYQQEIRKQKRAMLFQQGRQQERE"
     gene            complement(9293..11665)
                     /locus_tag="SCL80_002219"
     CDS             complement(9293..11665)
                     /locus_tag="SCL80_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108066.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQRKTNGQ
                     PMNDKRNGHSWFRQQERLENKVHSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIKEEIERFEKGESTFSAATIRKYQRKLETLEQLKERSEKGKENLLPEVQAI
                     IDSGRVTQWKKNPTIYFLKDYRKVALELNENGGFEESKKYQATSEEEKAAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQTVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTVETAEQKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNSFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
BASE COUNT         2892 a         2433 c         1958 g         4386 t
ORIGIN      
        1 gtgctatcac ttttagtttt taggataaaa agtgacgtca ctttttgagt gctggtacct
       61 caaatcctgc tttttcttgg gcaatcaata aatctaacgc aatacacata atcctggatt
      121 cacttttgcc gtacttctgc tttagttgct gcaattcttt ttgtctcttt tcatccaacg
      181 tcacgggtaa ccgtttattt cctattgcaa ttggcattag tactctctcc tttcatcgaa
      241 ataaccctat taaaaatatg gctgcaatca acttgaatat tagactcaca agggttaaca
      301 caaagcgcca aaagatcaaa caaaatacca gtattcctaa gaatactacg aagtttaaat
      361 gtagagctgc accatgacgc ataagtatct cgccaacaaa cagtacgaac aatagaggaa
      421 tgattttatt catatggatc tccttctttc tcttaggtta tcgggctttg cccgcttatt
      481 tcgcaccacc taagcggcgg gggcggttgg atcgattggt gcgaaataag aaccattccc
      541 ttaatttcaa ggttttttgt tcttatttca cacatcctca cttttcggta taaatggtta
      601 ttgacctttt ttcctttcaa atcgttgata tggcattcgt ttacggtcaa gttcattttg
      661 gatttcttct gtatcgtcgt aagtgtaggt gtctaaaaaa tcagttaaag tcgtgtacca
      721 gtcgtcgcct gggttatcta cttcttctac atattccctc aaaaatgctt cgggtacagt
      781 gagttcataa tccattccca ttaacacgat agattccgtc ccacacgtca aaaatctagt
      841 aaatacaagg taataatcgt ctaccgtcgc atctttgctg tttagctctt ccatttcttc
      901 ttccattaat tccatgctct caaaaattga ataatattct gattctgagt aatagtgttt
      961 cctgcaaatc gtgtagctga caaagttttc gaccagctca ctcctttcta ggtaaacatt
     1021 catgatatat ttttctgttt ctagtcgtag aagctccgct ccttttttta atgtgtcagg
     1081 acgacgttta tacacgtcta aacgattatg gctgactgta accttcgttt gactcgtttc
     1141 actttccata tacaactgtt cttccactct ttgaataaag tacttcattt cttccataca
     1201 actaaagtct ttttgaatcc gtctgattac ttcattcaag tgttctttgt ttgttaaaaa
     1261 ttgcgaaaca tcttctgttg ttagctgata aaccacctta ttcattatct ttctcctccg
     1321 attcaatcgt ataaaatgca ccattttttt cgatattttc aaaagtatct agccagtcgc
     1381 tagccattgc tttagctttc ttaatctgtg cgaattttgc ataagtcagt tctaaggctt
     1441 gatattcgga aggtttgata gtgcttggtt tgacacctac ttccaagcat agtaaggctt
     1501 ggaaaaacca ttttccattt tgttttttca gttcttccag tgttcgatag tacgtcgcaa
     1561 tgagattcat ttcgacacct gattcaatca tttcttccac tgcgactgac aatttttcct
     1621 tgtaatgttt tttgggtaca cgtgtttcat ggtataggaa cgtcatatcc tccatttttt
     1681 gtgccatttt atccaaaggt aatttgacaa attcgtcata gtaattttga gccatatttt
     1741 ttctcctttt tagttctcta atttggttaa ccaatattcg atttcataag tttccatgtt
     1801 ttcttgataa tgcagttcat atcctgcaat cttcactagg tcttcaaata gcatagtcgg
     1861 tgcatcctct actaaagggg tttctatcgt ccagtaccgc ggaataagta aagcttctgc
     1921 tactttttca gaaatgactt ctttgtccac caaaatttga acgacttctt ttacagtcaa
     1981 taatttgtgt tgtgttttca tcaccgtttc tttactttct catcaatcaa aacataatcg
     2041 ccataagaca aggtatattt tttcccgcat gttggacatt gatacaaacg atagcttttg
     2101 atacatttcg ctctttctcc acaatcgcat tcgacagtat gtggtgcatg atagtttggg
     2161 aataattcgc tgaagggaac accccaataa tcaaatttct ttcgcatgat tgattctgcc
     2221 tcctcttagg aaaggtttta tttcctttcg ttagttcttt ctttattgat ttgttggcca
     2281 gaatgaatca acgaaaagcg acaaggacaa gggcgcgcag cgtgccattt acccttgtcc
     2341 ttgtcgcttt ttggtgataa aaggcaactt gttgccggtc tggcaaatca ataaagagat
     2401 agcgaacagg aacgaacctt ttggtgagag gaagtttttt ctattttgtc ttgctttgca
     2461 agacaccata tcttagtgaa cgtgcaacgt tcacttcctt tcttctcgaa cggcggtggc
     2521 ggttggagtg agagaaaggt ttttcaattg agaaggcact tgtgtcttcc taaaaaggtc
     2581 ttttcctgtt tctatgtaaa acaaaaagag ctgcaatcct catttaggac tgcagctctt
     2641 cttttatatg cccatcgatc gcgtaaaatg atcggggttt ctaatttgca tcgattcaat
     2701 gactaattga ttccgtttat tgaaatgacc tgtgacggca atattgtatt ttccatcatc
     2761 cataattaac aatttatcga taatttcaat atctgcaact acacaattaa tcgattcatg
     2821 aattgtttgt aaagtaaagc gaaccaataa tggttgtgtt tttattactc ggattttatc
     2881 aagcaaacca acatatttgt tcatcacgat cacctcgcta aaattatagc aaaaacagct
     2941 aaaattgaga atttttattc gattttagat atgctataat gatcaagaca gtaaagtttg
     3001 tgacggtggc tatccgattg aaagtgaggt ggtgcgtatg tgcgtacttc caaaatctta
     3061 cagaaaggag aagccttttg cctgttgaaa cggcgctagg attaatgatt agtttcggtt
     3121 ctttgatcgc aacaatcatt tttggaatct tagcagttgt aaacaacaat caaaaaaaat
     3181 aaccgtcagc tttagccggt cgacgattat ttttcaaaaa taatattaag ctggtcaccg
     3241 tcttaaacgg tgctgttagg ggagttgtta gcgcaactcc tcttttcgta tccattataa
     3301 caactttatt gatcttttac aatcagaatg gtaaatcatc atcactaata tcgattgatt
     3361 gtccgccaaa tggattttgt gaagcgttca atccattgtt gttaggcgta gctacatccc
     3421 gttgttcaaa ggtggtaggg cttggactgc tggacgattg atcagcgtgc gcacggcttt
     3481 ctgtcgctgc tttactttct aatagttgga aattatcggc cacaacttct gtcacataca
     3541 cacgttgtcc ttgttggtta tcgtaggttc gggtttgaat ccgtccaaca acgccgatca
     3601 aactgccctt tttagctaat gtcgccatcg tttctgctgg tttgcgccaa atcacacagt
     3661 taataaaatc agcttctcga ttgccgtttt gattcgtgaa atttcggttg acagctaggg
     3721 taaaagtcgc tactgcagta ccactcgcgg tataacgtaa atctggatct ttggttaagc
     3781 gtcctactaa agttacatta ttgatcatct ttatctctcc atttcttgat tcatttgttg
     3841 tgatctttta aactgccgtt ttctttcttg attttcaatt ttgatcacac ttttcattag
     3901 gtgatcaatt tcgttgactg tatctgtaaa tgaaatggaa ttcgcaaacg tgttaactga
     3961 gcgatatttt tcccactgct tttccaaata gtcatttcct ctgatcttat ccatttctct
     4021 ttcataataa aaggacaacg gttttgtatc cccacgtttt gtaaatgtat ttcgaacact
     4081 tttgtataac tctttcgtat cgatattttc tagtttcgga atggtaaata agtcgtagta
     4141 atcttttgct ctcgaattat ctgtgccaaa gctgaacgta gcacttagct tttctgcaat
     4201 aatttgttct gttggataag ctggaatctc cactttttta tcttcaaaca ttaattggtg
     4261 atagtgtgta tctacttcag gataaataga atctcctgtt gtcacgtcca ttttaaacgg
     4321 tacacgtact ttctctaaat tagccaccaa tttaatttga aatccaggat aaaaatcggc
     4381 ttctcttgtt tccttcattc cttgaataga gaaaacaatg ccttcttttg tgggttttct
     4441 agttaaatca ttaaaaatgc tcttcaattt ttcttcagtt aatttgctat ttctaaatgt
     4501 cgtatcgata tcaattgttg tccttctatc gattccatat tttgatccta ataaaaagcc
     4561 gcctttcaaa atgaagtcat ttttgtactt tgattcagaa atttgttcaa ttagacgatc
     4621 catcataaaa tgccgttgca ctaactgtgc atttaatcct gtactctttg ctaaatttct
     4681 tgtttttgct gcaaattgtg tcggtgtcat ctttttcctc catcaatata agctttgcat
     4741 gtggtatttc atttcttttt ctacatgtag ttttgtcatg taatctctta atttacttgg
     4801 atctctcaat gggtcttcca tgtactcttt taatgcttct aatttcattt catattcaaa
     4861 ttcatatctc ggattccaaa tatcacaaag tgtacgctct ttatcataca tagtaatagg
     4921 atttccttga aaggaagttc cctctacttt tcctagttca taaacttctt tcttcacgaa
     4981 atgaaattgt aactgctgct cttttgtaat tgaacgatca acgttatagc catggggaaa
     5041 agttaagtga atgaatttcg gcatattcgt agaaagctgg taaatatcta aagtcgtttc
     5101 atgagagaag attcccctgg aaaatcgttg ctgaagccaa aataaatcat ctattccatg
     5161 accatcctct acataataac cacgatcaac tttgatcaat tttccttgat tcaccaatct
     5221 ttgtacagtg gttgctgcga ttccaaattt cctggcttcc ttcatagata aatttccatc
     5281 gcgaatagcc aacaattctt tcagtttatc ttgcatttac ttcccctccc ttcgttacaa
     5341 atacactaca tcaaatatat catagtagtt tatttgtaac aagattaacg tctatcgatc
     5401 taattcgatt tctttttgaa tgcgctgctg gttctttaat tgttgcagtc ttcttttatt
     5461 tgcacgtaat ttctcttgtt ctaaacgaat ttttgctttt tgattttcga tttcttgttg
     5521 tttgtgtgtt gtatttcgca taggataacc ccttcattat tttgtagtgc ggcgtgttct
     5581 tttcttatag ttcgtttttt tcttttcaga aaattcaaag cctgttgtaa agtcttcttt
     5641 taaaacgatc gttgcttcaa acgaaccttt ctgacctttt agtcctctga ctgattggct
     5701 tgttcggccg ttttgaatga tctctttcag ttgtgtggct gtgagtttct ttttggcaat
     5761 cgttgtccat aatttaaaat cacaggcttc agacgtacat gccgccacat tttgataaag
     5821 caagacttct tgttcccgac attttggaca aacacctaaa tgacgcgttg cttcctgttt
     5881 ttcactggct gctttcacct cttgtaacgt tgatccaaaa tctgttgact ggatttgtcc
     5941 cggtacgact tccaacaagt gagagacaaa tttttggata ttagctaaga aagtagcttg
     6001 tgttcctttc tgctcaccaa tctttaataa gtaactttcc catttggcgg tcatttcggc
     6061 acttgttagc agcggctctt tttccacggc ttggcaaagt aaaatccctt tatttgtcac
     6121 cacaagctta tttttttcta ccttgatata ttccttttgt ttcaaagttt caataatatt
     6181 tgctcgtgtt gcttctgtac cgattccttc tacttctttc aatatggatt gtgcttcttc
     6241 gctatccacg gtttttccag ctgttttcat cgctgtgatc aacgtacctt cggtataagg
     6301 ttgtggtggc ttcgtttctt tttcaaggac ttgtagatct accgtcaccg cttctccttt
     6361 tgtcacttta ggaagtagcg gttcttcttc cttttctttt ctgtcgctct ctttgccaaa
     6421 cagctttttc caacctagat ctactggtac ttttccgact gtctttagct gaagctgctg
     6481 aacttgcgtc aagatcgttg tttcttcata ggtatacttt tccgcaaaca tggccaccgt
     6541 tgtttttaac acttgttcat aaatagcctg ctgcagtgga cttaattgtt caaaagccgc
     6601 tgccgtcatg accgtctttg taggaatgat cgcgtaatgt tcctggactt tcttattatt
     6661 cacatatcga ctattcggtg tatgaatagg agtaggaatg gcttcggcct ttaaaaagtg
     6721 tttgtattcg tctaagtgat ccaacagata cgcatactcc ccttctgtaa tataaggggt
     6781 atctgttcgt gggtagctta gatatttccc ttcatacaac ccttgcatgg cttccaaagt
     6841 atctttggca ctagctttca tgagctgatt cattttagat tgaaggctgg atagagaaaa
     6901 aagacgtgga cttgccgcac tttttttctc ggtttcaact tggtggatcg tacctggctg
     6961 ttttcccaat tgaaccccct tttcttttaa ataatcttca aatgcttctt gcgtcgcttg
     7021 ggtttcattc ggatcaagct tcgcatcaaa tgcgccattt tgagcgatta cttgggcttt
     7081 tccttcaaaa tatggttctt tttggaagtt tctaatcttt tcctgtaatt gataaatcat
     7141 gtaaagcgtt ggggtttgga ctctgcctaa ggaaaaactt cctggtatgc ctttttgctg
     7201 caagagtaaa ctatacagcg ggctaccgtt catgccgatc aaccaatcgg cgatctggcg
     7261 tgcttgtgct tcttcaaatt ttgggtaggt ctcttctgca tctcgtaact gctggaatcc
     7321 ctgataaatg gcttcttttt ctaaggagtt gatccaaagt cttttaaata tctttccatg
     7381 atcggcgcct gcttgttgaa tgatcgtcca agctatcagt tccccttcac gatcgctatc
     7441 tgtcgcaata ataatggtgt cggccgattg aagttgctgt ttcaccacct tgaattgctt
     7501 tttcttatct ttaggaacat gatagtgata atgtgtaggg aaaataggca aatgctctaa
     7561 tgaccattgt ttccaatttt catcgtacat atcgggcgaa tctaactcga ctaaatgacc
     7621 aaaaccatac gtgatcacag tttctcctga aaacaatcga tcttggatct caaaataccc
     7681 atcttttcgg gtagctttag aaaagctatc ggcataagcc ttcgcttgtg acggtttttc
     7741 tgctaaaata acggttttca tcatgacgat ttcactccct ttcttgctgt ctaccttgtt
     7801 gaaacaacat ggcacgtttt tgctttctga tctcttgttg gtagatttca tgcttcaatt
     7861 gatcattcca atcctttttt acttcaggta caaattcatg ttcatgattt ttatagcctt
     7921 tgtagtattg ttctgcttta taagagaaaa gaatctgagc attaatatcc cctttttctt
     7981 gataaaaacg atcaaaatcg tacgtaccat ccacgcgttc acaagctttt aattcttttg
     8041 cgacttctct acaaatatct ttcaaattaa tagcttctat tgaatcgtta acagaaagct
     8101 gctgaccaaa agacgcattt actttgtgag cggaaaccaa ttggttcgtt aattgcagat
     8161 tgttttctgt cttgtgtatg gccataatag cttctggtgt gatcttttct tcttttgcga
     8221 cttcttcata aatgactttg tattcttttg gcacttgcca atcattagcg attcctcgtt
     8281 cacaacgtac tttctgattc gtaaatggat caactgcgcc cattaattgt tctttttcgt
     8341 agaaaatatg tcctgcacgg tcattatcca cacaaatctc gatggattga ggaaaggctt
     8401 gtttctttct caattcagat actgcctcac caaaataatg actgatcaca tggtgtttta
     8461 acccttccat cgaaactaac cacgtatcgt ttaactgatc acgatttagt gcagcatagc
     8521 taagtaaatc aatactactc tcaaaaaatt ttaactgctt agggtcaccg atctttaggt
     8581 taaagccatg attcgctgtc gaatttttat ctatatgctt atatgtgcct ctctcaccaa
     8641 aacgttcata atcaatgaac gttccttgat aactcgcccc tactgtctca tttgtctgtg
     8701 gatctttcca taaaaaggcg accgcatgat ctaaacgatc gtctcgaact aagaaatttt
     8761 gccgttcatg ttttggcttt agttcggcaa tcaacccttc tttttctgca taatcaatca
     8821 gccactctgg tagttttcgt tcttccttca gataattcct tcctttttct gtgagtggtt
     8881 gataatcaac ggggtgatct ttgtactttt ctgtctcaaa atgaagattt tccactcgtt
     8941 ctgttttgtc ttcattcttt agaataaaat cagtggctgc tttaaattgt tgcttagggt
     9001 cttggatctc tttgtctatg aaaaaaagtt tagctaattc aataatgtcg ccactaatat
     9061 tttgactgtt ccaataaaaa cgttgttttc ttcgatcaaa aacaaatgaa tcatggtctt
     9121 ctaagcgata gtctcgtcct ttattgacta cagccattcc attgtttcga gcaaaatcca
     9181 ctatatcgat ctgtttacat cgatcaataa gactcatttt tttctcggtc attttaaaac
     9241 ctcccaaaag aaaaaagact atccgttttt cagatagtct tttttacttg ttttatacac
     9301 gttctttttg tgacaattgt tgttcataat agatttcatt ttttcgtttt gccacttctg
     9361 acactgtctc aagtgaaaat cggaatgaat ttcctagcgc aaattcttct gaaggtaacg
     9421 ccgtttggat ctcttttcca taactttttc cttttaaaat tacttgatta tgctgtggat
     9481 cttcaataaa cgccgcatcc ttcaaatgta acgttcgacc cgtatcttct tccattactt
     9541 tcccgtcttt cttgtcgatc caaagtgaac caacaggaac aatattcttt tgttctgccg
     9601 tttctacagt atttttatgc tcctgcttca cttctgctgg ttggatctct ggttctcttt
     9661 ctctgtattg attgatttca ttagaaatgg tttgaatgaa ttcatttgcg acacggctga
     9721 catcattcat gattttccca cgttccgcag cttccttatc atccaacgtc tgaccattct
     9781 tcgtccatgc tgcaatataa ggaatagctt tctccgaagt gtccattcca aaatgtttgc
     9841 acacaatata agaagtcatt tctgcctgca attctttagt tgcggtatct aattctgaaa
     9901 atttgtgcat tcttgcatgg gctaattcat gaatacttgt agctaaatgc tgcgtaggag
     9961 tatttcgagt attcatgacg atttcttctt cgccagtaaa ttcgttacga acatatgccc
    10021 cccgtgcttg tcctaattct ctttgataga cttcactttc tgtcatatct cgaatttgta
    10081 ttccaatatt gttggctacc gcttgaatcc cagcttgaag ttctgactgt ccttttttat
    10141 ccaattgaaa atcaaagaca cggttcggaa agattttcgg atagtcttca ggttttaact
    10201 gtgtttgtgt gatatcaaac actttttcaa tcgtgtagta aggttttttg atcacttcga
    10261 tttgaccact ttcgattcgt tttttatctt ctggtgtagc ttctgtttct cgaacaaagc
    10321 cacgtccttt ctcataaaat cctttgaccg tttttctgac aaagatcttg ttcccttgtt
    10381 cccctttttt gatatagatt ccttcttttt tcaacgcagc acgacctaaa caagccgttg
    10441 cacctggaaa ttgttcctca atcaataatt gatttctaaa ggatcgttct ggaaaacgag
    10501 acataaaatt caaaagttct ttcacgtctt caggagaatg ggtatgcttt ttcaccgcat
    10561 ccagtgcctg ttgcgaaagg gatttgactt cttgcttaat ctcttctgcc gttcgttttt
    10621 gatagccctt ttgttttgtt tttgtttctt tcgactgcat ttgttctttc gaaacaggtt
    10681 catttttgac cgtttgtttt tgttcctttt caggtacttg ttcgcacaat tttgctaact
    10741 ctaaatagat gggttgatta ctgttgacgc catcccctag atccattcgg atctgatcga
    10801 ctacttctcc gttcttcact tcttcaaaat agaatttata ataacctgct tctgcggcgt
    10861 attggttatc caacgttttg agttgatcga tcaattctaa tgttacgatc tccccaccat
    10921 aacttttcaa tcctttttct ggatcatttt cgttgaattc tactcgatag aaatgatcgt
    10981 tttctttctc cttcaaagaa tcagttggcg cagcctcttc aagtaattca cgaacagctg
    11041 ctttttcctc ttccgacgtg gcttggtatt tttttgattc ctcaaatccc ccgttttcat
    11101 tcagttctaa agcaactttt cgatagtctt tcaaaaaata aatggttgga ttttttttcc
    11161 attgcgtcac tcgtccgctg tcaataattg cttgtacttc aggaagaaga ttttcttttc
    11221 ctttttctga tctttctttc agctgttcta atgtttccaa tttccgttgg tattttcgga
    11281 tcgtagcggc tgaaaaagtc gattcacctt tttcaaagcg ttcgatttct tctttgattc
    11341 ttggaatatt ttcgattgtc atatccaggc cgccgtactt gttatagccc atttccttta
    11401 gctcttcttg tctttcaagt ttttctacct gtttttcttg ttgcttgatc tcttctctca
    11461 gactatgcac tttgttttct aaacgttctt gttgcctaaa ccaagaatgc ccatttctct
    11521 tatcgttcat tggctgaccg ttggtctttc tctggtgttc aaacatcttc tcaatttgtt
    11581 cgtttaattc attttgcaat cgtactaatt ttcttttgga ttttcccaag cgactattgt
    11641 ctagtggttc tttttcttct gccatgtcc
//
LOCUS       DARBEC010000083        11641 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.086, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000083 DARBEC010000000
VERSION     DARBEC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11641)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11641)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11641
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.086"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(105..629)
                     /locus_tag="SCL80_002220"
     CDS             complement(105..629)
                     /locus_tag="SCL80_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108067.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCL80_002221"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="SCL80_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEG1108068.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
     gene            complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCL80_002222"
     CDS             complement(1218..2198)
                     /gene="pta"
                     /locus_tag="SCL80_002222"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEG1108069.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(2351..3031)
                     /locus_tag="SCL80_002223"
     CDS             complement(2351..3031)
                     /locus_tag="SCL80_002223"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEG1108070.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            3157..4026
                     /locus_tag="SCL80_002224"
     CDS             3157..4026
                     /locus_tag="SCL80_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEG1108071.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            4052..4567
                     /locus_tag="SCL80_002225"
     CDS             4052..4567
                     /locus_tag="SCL80_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108072.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(4614..5207)
                     /locus_tag="SCL80_002226"
     CDS             complement(4614..5207)
                     /locus_tag="SCL80_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108073.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(5232..5411)
                     /locus_tag="SCL80_002227"
     CDS             complement(5232..5411)
                     /locus_tag="SCL80_002227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108074.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(5488..5997)
                     /locus_tag="SCL80_002228"
     CDS             complement(5488..5997)
                     /locus_tag="SCL80_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108075.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(6093..6473)
                     /locus_tag="SCL80_002229"
     CDS             complement(6093..6473)
                     /locus_tag="SCL80_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1108076.1"
                     /translation="MNLTTIHHIAIIVSDYQKSRDFYVNKLGFEVIRENYRKERDDYK
                     LDLKLGSAELEIFGIANAPQRPNYPEACGLRHLAFKVDDIEETIAELQSLGIESEPIR
                     EDTFTGKKMTFFFDPDGLPLELHE"
     gene            6803..7552
                     /locus_tag="SCL80_002230"
     CDS             6803..7552
                     /locus_tag="SCL80_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEG1108077.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(7736..9013)
                     /locus_tag="SCL80_002231"
     CDS             complement(7736..9013)
                     /locus_tag="SCL80_002231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108078.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCL80_002232"
     CDS             complement(9092..9736)
                     /gene="pcp"
                     /locus_tag="SCL80_002232"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEG1108079.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(9935..10879)
                     /locus_tag="SCL80_002233"
     CDS             complement(9935..10879)
                     /locus_tag="SCL80_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEG1108080.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(10881..11567)
                     /locus_tag="SCL80_002234"
     CDS             complement(10881..11567)
                     /locus_tag="SCL80_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEG1108081.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
BASE COUNT         3321 a         2466 c         2047 g         3807 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgtttttggc
     1201 tagttcgcgt gtatttgtta atcaatcaat gtttgtgctg ctgtgataat cgataattta
     1261 tatacatctt cttcatttgc tccacgagac aaatcagaaa ccggtttgtt caagccttgt
     1321 agaattggtc cgattgcttc aaagttaccg aaacgctgag caattttata gccgatattt
     1381 cctgactgca attctggaaa tacaaagact gttgcttgac cagctacttc tgaatctggc
     1441 gctttcagtt gtgcaactga aggaacataa gaagcatcaa attgcaattc gccgtcgatc
     1501 atgtattgtg gcgctagttt tttagcgatt ttagttgctt caactacttt gtctacttct
     1561 ggcgatttcg ctgagccttt tgtcgagaag ctcatcattg ctacttttgg ttcgatatcg
     1621 aacaattctg ctgtttttgc agaatctacg gcgatttcag ccaattcttg cgcagttgga
     1681 ttcacgttga tcgcacaatc cgaaaagaca tatttttctt gatctcttcc acgtaccatg
     1741 atcatcgctc cgcttgtccg actgatgcca ggctttgttt tgatgatctg aagtgctggg
     1801 cgaacagtgt cgccagtcga atggatcgct ccagagacca ttccgtctgc aatccccata
     1861 tacgtaagca tcgtaccaaa gtaattcaca tcttttaaaa tcttttctgc ttgttctttg
     1921 gatgcctttc catttctacg ttcaacaaaa gtatccacca tttcatccca cttatcgtaa
     1981 ttatcagggc tgatgatcgt aaagctagat gttttgattc ctctggcatg cgctgcattt
     2041 cttatttctt cttcgttccc tactaaaatg ggttccacaa gttcttctgc tttcaaccgg
     2101 gcagctgcgc ctaaaatacg agcatcagta gcttccggga aaacgatttt gatcccacgg
     2161 cgaacaattt taaatcttaa actatcaaac aattccacaa gatgacctcc agacgttttt
     2221 atatgtatta tcatacacta ttccagaaaa aaagaacagt ttattttatc tgtatcagtc
     2281 aaaaatgctg gaaaaattaa gtttttcaca tacaaagcga actgcactat aacaggaaag
     2341 cgctgtttta ttacactgtt tcaggcagtt gccaatcaat cggttcttct cctaacgcaa
     2401 ggagcgcatc attcgttttc gagaagggtt tggaaccaaa aaagccacga cttgcagata
     2461 acggactggg atgtggagat gttatgatga tatggcgact cttatcgatc attttgattt
     2521 tttcttgtgc cggttttccc cacaagataa acacgattgg tttttcccgt tcatttaatt
     2581 tttcaatgat cgtgtctgtt aatctttccc aacctttccc gcgatgagaa tacgcttgcc
     2641 cttcccgaac ggtaagaacg gtgttgagta aaaggactcc ctgtttagcc catgacacta
     2701 gatttccatg ttgaacaggt gcgattccaa gatcatcata aagctcttta tagatatttc
     2761 ttaacgatgg cgggatcttt acgccaggtt gcacagaaaa agataagcca tgagcttgat
     2821 tttctccatg ataaggatct tgtcctaaaa tcaccacttt aacttcttca taaggagtca
     2881 attccaatgc ttcatagata tgatacatat ctggatggat cttttgggac gcatattctt
     2941 tttttaaaaa ttctcttaga tgcagataat aatcttttga aaattctgag gacaaaactt
     3001 cttgccaact gttatgaatg atttctttca aaaactccac ctcctaataa aaagaataac
     3061 tcacattttc ttcaatcaca agggacaatc ttcaccctca gcacttgatt aatatggtaa
     3121 actaggaaca agaagcagaa aggaacgata atcattatga tcaaattaat tgcatcagat
     3181 atggacggca cattattaga tgcccatatg agtgtttctc ctgaaaatgc cgaggcgata
     3241 cgttttgcaa atgatgccgg tgttgagttc atggtcgcta ctggtcgaaa ctatcaagaa
     3301 gctcgcgcag ctttagatga ggtaggaatc gattgtgcga tgatcacttt gaacggggca
     3361 caggtatttg ataaagaagg aaattcattg tttaccgtgt cgattccaaa taatcaagca
     3421 atctctgttt tagatattct agatgctaac ggtatttact atgaagtagc aaccaacgat
     3481 ggtctttatt ctgaaaatca agcgaaacga attgaaagtt ttgcttccat ggtggctacc
     3541 catttacctc accttacgta taaaatggcg attgccatgg cctcagctaa gctggagtta
     3601 ttgcacatca catacgtgga cagtattcgt gctattttgg aagaaaagaa tctggaagtt
     3661 ttaaaaatta tttgttttca tacagaaggg ccagctgtat taggtccagt tggaaagcag
     3721 atttctcttt taggcgatct agccgttact tcctctggtc aaaacaatat cgaagtgaac
     3781 cataaaaacg ctcaaaaagg aattgctgtt gcccatgtcg ctcacgagcg tgggatttct
     3841 ttagaagaag tcatgacgat tggcgataac ttcaatgatg tcagcatgct gcaagtagca
     3901 ggagtcagtt tcgcaatggg aaatgcagaa atcgaagtaa aagattatgc caaatataca
     3961 acggacacaa accttgaatc tggcgtagga aaagccatat tgcgcgcgat tgcagaagac
     4021 ttgtagtttt atctagaaaa gagggatata gatgtcagaa tttttcattc aggaaaagca
     4081 gcttggacgt gcaactcgga caattgtcaa aaacgaaaaa ggacattccc tgtttttgat
     4141 ggttggtcgc tggggaacaa agggcgatgc attgtctctt tatgccatga atggcgatct
     4201 cgttgctcac atcaagcaga taagcctgac attcggtacg cgttttgagc tgtataaaga
     4261 gttcgaaaag gtgggaacca tgcagaaaat attcaattgg cccggtgact tttattatat
     4321 tcgtagactt cactggacag tccaaggtga tatctataat catcaatatc aaatccatca
     4381 tttcaatcag caaatcatga aaatggataa agcaacactt ttttcaggag attactatgt
     4441 attggatgtc tcaaatgatg aagatgctcc agtttgtatt tgtattgctg cggttttaga
     4501 ttactggctc taccgtaaag acaaaagtcg cgataagcct gtttcttacc gacttaactt
     4561 aagttaaaaa gatgtacctg aaatgcggaa atacgcattt caggtacatc tttttagata
     4621 ttcttttgca tatgatcata aagatgacca ctgatcatcc gctgaccgac aactttgaag
     4681 tcatggcgtt cgtataattt tttagccttt ggattttttt catccacact tagaccgatg
     4741 acggagcgat tagctttctt cgccaattta ggtaatgctt ctaataatcg cgaaccgatg
     4801 ccttgtcctc taaaatcttc tgatacactg atagagtcca aataccattc gttgggaaac
     4861 gtttctttat caatgaacaa acgtgtagac gcatctagct gatatttctc taagataggt
     4921 accagtggct gatcgatgat tgattcttct tcatctgtat aaccgtaagc aactcctgca
     4981 actttaccgt ctatttcttc tacgatacct cgtgaataac tgtaacgata atttggatca
     5041 gtgattgctt cttctaaaat ctctaaagtc ttttgttccc ctgtctgctt caaaaatggc
     5101 agttccatat cttttagaat cactaagatc aatggggcga tttctggagc atcttctttt
     5161 ctcgcaaaac gaatcatagt ttcctctcct tttctccatt ctaacatgca gaaaaagaaa
     5221 tcgcaaaatc gttattttaa aaaatcttca atcttttcag taatgaaata accatgagta
     5281 acgattcttg ccgcttcagt tcggcaataa gggcagacga ctggcatttc tttatcatta
     5341 tgttcgacta cattattgac ttcttcgtat gcaaattttt ttccgcaatt agggcatttc
     5401 attttttcca tgttatctca acttcctttc atctttactc tcttagctta ctcttatatt
     5461 gtaaaaaaga gaagcaatat ccttcttcta tgcagatatc catacctgac ggtcctgttc
     5521 tccatctatt aatcggactt tataataagg acgttcatct ttgactagaa gtgtccactc
     5581 tttcactttt gcttttttcc cgtagtagct gactgcttcc ttcatcgctg cttgcggttg
     5641 tttgaactct tccgtttttt ctatatcaaa aagatgttct gcggcctttt ccgtgtcatt
     5701 tcgtacggtt gttttcccag tggatggatt gactgctatg acttgtcctt cacaaaaaaa
     5761 ctggtattca tatgttgcat tcgtaggatt ctccaaatct ttttcaactg caaaatgaat
     5821 agcagatatg ttatgttctt ttgtttcgtt ttttacgatt gctgctgctt ttttcaatga
     5881 gatccgatat tcctggtcac taatatcatc catgtgaaaa gcggcattga gcagtcccgt
     5941 cataccggta atccctatta cgatgagggc agcaacacca aacatcattt ttttcattct
     6001 tcattctcct gtttcttttt tttaaagaat atcaagaaat gaacgaatga aaaatcgaat
     6061 gaaacgaaaa aaaagtgaca attttgttat ctttattcgt gcagttctaa tggcaatccg
     6121 tctggatcaa agaaaaacgt catttttttt cctgtaaagg tatcttctct aatcggttct
     6181 gattcgatcc ctaaggactg caattctgca attgtttctt caatatcgtc tactttgaaa
     6241 gcaagatgac gcaagccaca agcttctgga tagtttggtc gttgtggcgc attagcaatt
     6301 ccaaaaatct ccaattcagc acttcctagt ttcagatcta atttatagtc atctcgttct
     6361 ttgcggtaat tttctctgat tacttcaaat cctaatttat taacataaaa atcacgtgat
     6421 ttctgataat ctgaaacaat gatggctata tgatggatcg ttgtaaggtt catatgagca
     6481 ctcctattct tcgtttaagc tcggatctat attaatctcc agttccattg taaatgtgtc
     6541 agattgaaaa aaatagctgt ttttcctttt tataagaaaa aaggcaaaaa aaacagaaag
     6601 taggtcctcc aagacactct tcctgttccc aatataataa atgaaagtaa gaaattatat
     6661 tttcaagaaa acaaacagaa aattatcata acacaaatta tttataattg aaagactagt
     6721 tttttttcaa gaaaagcata caatagattg tgttagtaaa tacagaacac actacattta
     6781 gtgtcttaag gagtcctcat acatgaagtc atattttcag tttttagctc atcagctaaa
     6841 aggctggccg caacaaaatt attatctttt ctttttcagt cttggctgcc aggtcatgac
     6901 tcttgtcaat agtcccatca cttggctgtc cgttattaca tttatcggta ccacgcttgg
     6961 tgtattgtgt attttatcca tcaatgcggc aaaatcagtc aacggtattt taggtatttt
     7021 gtctgcgatt tgttttatta tcgttgggtt ttcagcaaag aactatttaa gtatcggtga
     7081 acagattgct tatatgatca cattggatat ccccgtattg ttaagtaaag aatggaatca
     7141 taatatggct tcaaaaatcc gaaaattcac tggaaaaagc tggtttatcg caatcatcag
     7201 tactttcctt gtctatcttg tttcaggatt cctgattggt cgttatacag atgatcctcg
     7261 gccgtgggta gatgccattg ctttttctat tagcttgact gctggtatca tcagtttctt
     7321 gcgttacaac aaccaatatt actggtggtt agcttcaggg cttgctcaaa tggtcctttg
     7381 gtatatttca tttacgcatg gatctgcaag tttggcgatg ttcgtgaaca gctctgttta
     7441 cttgctgaat gatgtccttg catttaccgt ttcaccttgg tataaccaaa aagaacgtga
     7501 acgtatgcaa aaaatggaag aagaatacta caacgcacaa caacacgcat aaaaaaactg
     7561 agcgtgggac aaaagtaaag aacacttttg tctcacgctc taaacacgta taaacggcgg
     7621 aatcagaagc aactccttcg gaaataagct gaaattcaca aaaatttgaa aagcaatttt
     7681 cgtgaatttc ttcttatttc ttggagttaa acgcttctgt cccagcctct attttttata
     7741 gtaattcctt cagatacttt cttgcctcat cattgaaacg cagtgattct tctaacttct
     7801 gataatattc caacgcttgt ttctgagctt cagtaaaacg ataatgtttt cctttccatt
     7861 tgtcgattgt ctcttgcaca tttccagatt ggatatcttt cactactagt tgccattttt
     7921 cttttgagac tgtacggtct ttcttatcag cagattgtat agcttctcgt tctttacgca
     7981 atacttttaa aaaaccattt ctgactatcc gaggaatgct gtcgaagaac ccttctgtaa
     8041 aaccatagcg acaatattgc ttccaaataa atagatagat ttttggttgg atgaatcgtt
     8101 ggtttttcgc aaaatattcc atctgagcag tgacttctgg tgttcgttga cgttttgtct
     8161 ttctcaacac ggaaataaat gtttcaaaat aagcattagg atgtactggc acttcttcat
     8221 atacgatgga ttgccaatct ttacgtaaaa atgcgcgata gttgatcaag aattcttcta
     8281 catcctttac taattcttct gcaaagtcgt caaatagttc tttttttatc tgtcgctttt
     8341 ttgactgccc attgatgtgt tcacttattt tcttgatttc attttccaat gcttgataat
     8401 ccgctatgta aatcggcatt tctacttggc gaaattctgc gagccgctga gctaatttta
     8461 tatccggtaa atatggctgt ttcatccgac gattttggta caagacaaaa cagtattttt
     8521 gccatagtcc ttcaggaaaa tcgatattct tctgcttaag ccataataat tcgtctaagg
     8581 ccagatcaac ttttgtcatt ccctgatgga tcgaagctgc cactgtcgga gagacatgca
     8641 ggtgatcggc aaagtggttg atgcctagtt ttaggatctt tccggttttt ggcgatttaa
     8701 cgatgtattg cacttttaat tctctaccac actcacaata caatttttgc tggcttcgtt
     8761 ttgttcgaaa aaacggattg atcttgaagt cagaaaacac ccaatctgtt gcagctaaat
     8821 aatgttcttc cataaaacga gagctgatta gatatcggcg gtgcttatcg actagttccc
     8881 gctgtttttt cgttaatttt gcatagatct cccaacgttg actgcttgtg tattgtttag
     8941 aaattttgat gggatagctt ttgctgattt cttttggaaa ccattcttca aagacggatt
     9001 gccattcttc cacggctgtt tctttccttt ctaattacaa aaagctggct actaacataa
     9061 cgttagcaac cagccagtgt ttcttgctaa attagtgagt tgttccggca tttccggaaa
     9121 tatcttcttt attttctact gctgcttcaa ctgcataagt caaagaagaa gcaatgtctt
     9181 ctaaagacat agaaggcgta cccactggac gatcgatcac ttgatcgggt gagaatggca
     9241 cgtggatgaa cccacctcga attgccggga attgtgtttc gatcaaatga agcaaacgat
     9301 acatgatatc attgcagaca aatgttcctg ctgtataaga gatgtacgct ggtagaccat
     9361 gatcttgtac attttttgtc attgctttga ttggcaatga ggtaaagtaa gcggctggac
     9421 cgttttcttc taaagccgta tcgaatggct gatttccttc attatcaggg attcttgctt
     9481 ctgctagatt gattgcgaca cgttcgaacg agactgctga ccgcccacct gcttgtccga
     9541 cacagatcac gacatctggt tggtgctgtt ggatggcttc tttgactact tgactgcttt
     9601 tttcgaatac ggtcggtact tccactttga tgatttcagc accgctgatt tcatctggca
     9661 ttttttttac tgcttcatag gctggattca ctttgtcccc accaaatgga tcaaaacctg
     9721 ttactagtac tttcatctgt tgttcctccg tccgattgat ttaacatatg gttatctttt
     9781 atcataccat tttccttgaa cagcgtaaat aaaggaaaca atagtttaaa taacataaca
     9841 gatcaaactt aatatattct tataaaaaaa tagtgttcca ctacaatgtt agaagaggtt
     9901 gcgacaatta ttctgtcaca acccctctct tctattattt aaatagaatc atataagcaa
     9961 tttggaaaac tagcataaat aacgcaataa acaactggtt tttgatcact ccgtatttgt
    10021 ctttcatctc cagcatcgct acaggcacga tattgaagtt agcagccatc ggtgtcagca
    10081 gggtcccaca aaaaccgcaa gtcaatgcaa ccattcctat taacgctgga ttcgctccat
    10141 gactaaatac gaatggtgca ccgatcccaa ccgtcatcac ggtgatggcc gcaaatgcat
    10201 tccccatgat taccgtaaat atcaccatgc caagagcgta aatgatgatg ccgatgttca
    10261 catttccttc tggaacgatc gtacctactg cattggaaat cactgtccca actccagcgc
    10321 ttgtaaatac cgcacctaga gaagctaaaa gcattggcaa catgcttaac ggccctactg
    10381 tgcccagcat atctgctgca tcatctaaaa atgtacttgg tcgattatct cgagaaaaga
    10441 acatcaagat tagcatggct gcaaaaacac cgacgcccac gccgactaaa gcacctaaat
    10501 tggtaaatag agcaaacaag atcgcaaaga caccgatact caaagctgga ataaaaattt
    10561 tcattcctag tttgtctgac attttttcca tatattcttt tgatggaagc cggctttctc
    10621 ctttgctgac tttcttagca attgctggta tggtcatcgc aaagatcaag ccaccgttca
    10681 cgattgccgg aagccatctt cctcctgcaa taactacccc taagaccgtc cagaaaaagg
    10741 ctgacccata acgatgagga ttggttttat ctgttaaatt tcttactcca gtgtaaatca
    10801 atacgagtcc catcacgata taaaggatct ccagtaattt atcgcctaat aaaatatcag
    10861 gatttgtaaa aaagtccatc ttattgcccc tttcctggat aatatttctt cgctaatttc
    10921 ttttctttaa gatagaagta gacacttgcc acaactaaag ccgtgatagc cactggaact
    10981 tccactaaag caagctgtcc caatgttacg tcatatccta gatctttcag tgttgattgg
    11041 actagcaacg cccctgctcc tccgatgaat agtacttggc caaagaacca gcagacattt
    11101 tccatagctg aagccatacc ttttagttct tcttcatgct taggatggac ttctaaccct
    11161 tttgattcga tggtccccaa tgccatcggt aaaatgattg gacgaacaaa tccagcaact
    11221 ccaccaaaac tcacgttaaa agccgcaaag atcattcgaa agatcccata aatatctaca
    11281 atcataccag cagatacttt cttgaatcgc ttgatcaagg aagccgctga ttctttcaga
    11341 ccatttcttt caagcgttcc tgttgccaac ataatgatga taaaaatcgc catcccgcga
    11401 ttgtttacaa aacttgtacc taaaagttct aacattcctt gaatgccaag accgccagtc
    11461 aaagctgtaa caactaaagc aaccattacg atcaatatcg agtctaattt aaatgcaaac
    11521 ccgagaataa caatcaagac gcctaaaagg ctaagataat tttccatata ttttcctcca
    11581 taaaatgaca atattttttt aattataaag caaaacacat aaaaaatata gttgttttct
    11641 c
//
LOCUS       DARBEC010000084        11615 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.087, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000084 DARBEC010000000
VERSION     DARBEC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11615
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.087"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..305
                     /locus_tag="SCL80_002235"
     CDS             6..305
                     /locus_tag="SCL80_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1108082.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="SCL80_002236"
     CDS             378..1739
                     /locus_tag="SCL80_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEG1108083.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="SCL80_002237"
     CDS             1761..2021
                     /locus_tag="SCL80_002237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108084.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="SCL80_002238"
     CDS             2035..2787
                     /locus_tag="SCL80_002238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEG1108085.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="SCL80_002239"
     CDS             complement(2881..3486)
                     /locus_tag="SCL80_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1108086.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="SCL80_002240"
     CDS             complement(3539..4513)
                     /locus_tag="SCL80_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEG1108087.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="SCL80_002241"
     CDS             complement(4891..5610)
                     /locus_tag="SCL80_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1108088.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCL80_002242"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="SCL80_002242"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEG1108089.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWIVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="SCL80_002243"
     CDS             7161..8558
                     /locus_tag="SCL80_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1108090.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="SCL80_002244"
     CDS             8639..9079
                     /locus_tag="SCL80_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1108091.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="SCL80_002245"
     CDS             9227..9553
                     /locus_tag="SCL80_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1108092.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="SCL80_002246"
     CDS             9554..10303
                     /locus_tag="SCL80_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108093.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="SCL80_002247"
     CDS             complement(10383..10838)
                     /locus_tag="SCL80_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEG1108094.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="SCL80_002248"
     CDS             complement(11265..11540)
                     /locus_tag="SCL80_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108095.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
BASE COUNT         3668 a         2014 c         2360 g         3573 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aatccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaaa
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactg
//
LOCUS       DARBEC010000085        11598 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.088, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000085 DARBEC010000000
VERSION     DARBEC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.088"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            198..1004
                     /locus_tag="SCL80_002249"
     CDS             198..1004
                     /locus_tag="SCL80_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108096.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            1016..2236
                     /locus_tag="SCL80_002250"
     CDS             1016..2236
                     /locus_tag="SCL80_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108097.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            2226..3812
                     /locus_tag="SCL80_002251"
     CDS             2226..3812
                     /locus_tag="SCL80_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEG1108098.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            3851..5989
                     /locus_tag="SCL80_002252"
     CDS             3851..5989
                     /locus_tag="SCL80_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1108099.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKDGIGDFSLRPD"
     gene            complement(6357..7340)
                     /locus_tag="SCL80_002253"
     CDS             complement(6357..7340)
                     /locus_tag="SCL80_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEG1108100.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            7718..9109
                     /locus_tag="SCL80_002254"
     CDS             7718..9109
                     /locus_tag="SCL80_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1108101.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERSFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            9125..10165
                     /locus_tag="SCL80_002255"
     CDS             9125..10165
                     /locus_tag="SCL80_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1108102.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            10184..11269
                     /locus_tag="SCL80_002256"
     CDS             10184..11269
                     /locus_tag="SCL80_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1108103.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            11302..>11598
                     /locus_tag="SCL80_002257"
     CDS             11302..>11598
                     /locus_tag="SCL80_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1108104.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYP"
BASE COUNT         3957 a         1828 c         2266 g         3547 t
ORIGIN      
        1 acgagagagg ctgtgacaac cgtcgcagcc ttttttggtt tattgcaatt gtctagcatc
       61 ttctgtaaaa tagaaacgtt agagaaaatc ttgtatcata gaaaattaag aaagaaataa
      121 gaacgcagct cggtaccaaa aatatggtaa aatggcactt gactgtaatt cactgaaaat
      181 cgcttggagg aacacaaatg tttaaagatt tgtatttatt tatgaaggac atttatcaga
      241 acagaaaatt attgctacag ttttcgatca atgacttcaa atcaagatat gcaggatcgt
      301 ttttaggaat cttatgggcg tttatcaatc cgctatttac tgtattgatt tattggctgg
      361 tatttggtgt tggactgaaa gcacaaatga tcgatgcaaa atacccattt atcgtatatt
      421 tagttaccgg gatgattccc tggttcttct tctcagatgc atttgcatca actacggttg
      481 tttttcggga atatacttat ttagtaaaga aagtcgtatt caatattcgt atcttgccca
      541 cgacgaaaat cctatccaat ctgtacaccc atttattctt cattctgatt ggcatagctg
      601 tcgcgcttat aaatggtata tatccttcag caatgtcttt acagctgatt tattatttat
      661 tttgcatgtg tgctttcttg acaggattga cttggctgac ggcatcgatc caaccatttt
      721 tacctgatat catgcaatta attacgatcg tgctgcagtt gatcatgtgg acattaccga
      781 tattatggag ccctaatcag tttgattcag gtcttgtaag catattgaaa ttaaatccgc
      841 tgtactatgt tgttcaaggt tatcgtgagt catttttaag tgaagcttgg ttctgggagc
      901 atggaacata tactttgtat ttctgggttt tcacgttagt aatgttcgta atcggctctg
      961 cagtgttccg tagattgaaa ccacattttt cagatgtgct gtaaggagaa tagctatgac
     1021 agaatatgca attagactaa aagatatcac aaaatcttat aatatgtacg caaaaccttc
     1081 ggatcgattt cgtgaagccc tcaatccttt taagaaatcc tatcatgatg tattttatgc
     1141 gttgaaaaac gtgaatgtag aaataaaaaa aggtgagatg atcggtttcg tgggagaaaa
     1201 cggatcggga aaatctacaa tgttgaaaat cattacggga gtactgacac caacatccgg
     1261 cacgatggaa atcgaaggaa aaatctcagc tttgctagaa ctaggatcag gattcaatcc
     1321 tgagtattct ggctatgaaa atatctattt aaatggaatg gttctaggat tttcaagaga
     1381 agaagtcgat gaaatggtcg atgatatcat tgaatttgct gacatcggag accatattta
     1441 tcaacctgta aaaacttatt caagcgggat gttcgttcgt ctagcttttg ctgtagcaat
     1501 caatgtgaat cccgatattt tgattgtgga tgaagcattg gcagtaggcg atcttgaatt
     1561 tcaactaaaa tgtatggaaa aatttacaga aatcaagaac tcgggaaaga cgattttatt
     1621 cgtttctcat gatatcagtt cgattcgtcg tttttgtgat cgttcctatt ggttgaaaaa
     1681 cggagaagtc gtcgaatatg gcgatacgat ggaagtaacg acgaattatg aaaacttttt
     1741 aaagaaaaaa tcagttaaaa cagtggatcg gaataaaaac caagtagaac ggttcgccgc
     1801 tccagatatc gtcgacgtga cgaaagcgga attgttggat aaagacaaaa atccggtgga
     1861 tatgctggaa caaggagaaa aactatacgt taaagtaact tatgaagtaa aagatgatac
     1921 aatcaaaaat ccggttttag gaattgcgct acgaacagtc gacaattttt atgtatgcgg
     1981 acttaatact ctactagatg gtatcaagat tccttggaaa aaaggagaga atatctttta
     2041 tctggagtat gaaaagttag gtttgttagc tggtgaatat tattttgacg ttgcggtctt
     2101 tgaagaaaat gcaacggttc ctttggtata taagacaaag tacatgaact tgtttgtcag
     2161 cggttcttat atcggagaag gcatcgtggt cttagaccat aaatgggaag aagggactca
     2221 tagcaatgaa atatgatttt gaaatggacc tagatgaaca aagcagtgtg ggaaaaattg
     2281 ccgcacagat caaaccagga agtaaagttt tagagtttgg tcccggaaac ggccgattaa
     2341 cgaaacattt aatcggagca aaacaatgtg aagtgagcat tgttgagtta gataaggaat
     2401 tgttcgattt tgtcagcgaa tttgctcaag atggatttta tggtgatatc gaaagttttg
     2461 agtgggcaaa ctattatgct ggacagacat ttgattacgt cttgtttgcg gatgttttag
     2521 aacatttggt agatcctgga aaaacattga agaaagtcag agaatttttg aatgaagaag
     2581 gagagatcct gattacattc cctaacttgg cgcataattc tgtcatgatc gatttgttca
     2641 ataaccaatt gccgtgggct tcttatggtc tcctagatga aacccataat tctttttata
     2701 ctcatgatgg cttccaaaaa gtttttgaaa aagcgggatt attcattaat attgaagatt
     2761 atttgtactt agctgtaggt gatacagaat taaaatcaac ttatgaagaa ttgccggaag
     2821 ctgtacgcta tgatttcaaa atgcgaccat ttggtgaagt ctaccaatac tttttctcat
     2881 tgatgaaaca tccagtagca caaagttcca ttgcagagcc acaaaattct aattatgtaa
     2941 aagtgttaga agtaacccag caaacgaaac aagatgaaac aatccaacaa ataccattca
     3001 ataatttcac aggggaaaat gagacactaa gtttccccgt atcagaaaca acagaaagaa
     3061 tggtgtttcg cttcgcgcaa caacctagct ttatcgaatt cagcgcagaa gctgccggag
     3121 aaaaactaac atttatcgat tcgaatgctg tagtcaaaac agtaaatgat tgttatttgt
     3181 ttgatgggaa agaattacca gagttcgttc taacggatat ttctgggaaa gaagtgacta
     3241 tccattgtca ctatcgcttt atcggggaat tgacaccaac aatgaaagaa ttgttggaaa
     3301 cgattcgtcc catggctgaa gtcacaaagc agctttctga gaaaaatgac gaattagaaa
     3361 gagaaaatca tcatcttctt gaggaaaata caagactaga ccatacgtta aaaacaacca
     3421 caaatcgcta ttgcacattg ttggaaagtg atgaatggac aataaaacat agactaggac
     3481 gacgtaagaa agaaacttcg aaaaaaatcc aagaaaaaga actttctata tgtattgacg
     3541 agaaaatctg ggatgcagaa acaaaaatat taaaaatcat cggatgggga atcgctaatt
     3601 cggatcgcca gcccttgtcc tataagttat cagctgacca atcgccattt ttcgaagcaa
     3661 ttcctgtttc acgagaagaa gtcaatcaag cagaacagct cgcaaaagga acagaagcgg
     3721 gatttgagtt acgaatcctt tgcgagcaag aacgttcatt cttagtggaa gcagtagctc
     3781 aaaatggcca atcatggatc attgaaatgt aaataatgat taacttataa agataaggag
     3841 taaacgaatc gtgcaaaaaa aagaaattgc cgtatttatc gatcaaatca ctcgtgaacg
     3901 ggccactggc gatttgctta ttgtgggctg ggcaattgat gaagtgacca aagaaatccc
     3961 tacgataaag gtagaaaaag aaaatgtgat tgctgaagca acacatgtcg taagattgga
     4021 tatcaatcat ttatataatc ttgatgttaa gacccaaagc ggtttcaagg tacgtttatc
     4081 agggaaaatg agaggaaaag ccatcttaga cttccaaaca gcgagacatc aaaatggcat
     4141 agctgtgaaa ttaaacggta aataccctta tgatgacggc attgaatctt catgggaaag
     4201 aaaaaaaaga ttactgaaaa aagggataaa ttatgcaagg actcatggcg taaaaaaagc
     4261 gatccgtcgg gtcaaattgg aattgaagcc aggatcgatc gactatgcag agtggatcag
     4321 tcgtcatgag aaaccagatt tgaaagagca aaggaaagaa gcacaaggat ttgcgtaccg
     4381 tccattgatt tccatactta tgcctgtcta taacgttgaa atcaaatggc tcgaaaaatg
     4441 cattgattcg gtactggatc aaacgtatga tcattgggaa ctatgtatca gtgatgatgc
     4501 ttctactgat ccagctatcc gaaagtgtct ggaatcctat caagcaaaag atgatagaat
     4561 caaagtcgtt ttccgaaaag aaaatggaca catcagtctg gcaactaatt ctgcattgga
     4621 aatagcagaa ggagaattta tagcactctt agataatgat gatgaattac cgccatttgc
     4681 attatatgaa gtggcaaaag tgctaaacgt acatccagaa ctggatgtga tctacagtga
     4741 cgaagataaa atcgatgcag atggcaatcg ctttgatccg catttcaaag cagactggtc
     4801 gcctgataca ttgatgggaa ataattatat ctctcactta ggcgtatatc gaacaagcat
     4861 tgttaaagaa cttggcggtt tccgtaaagg ctatgaaggt tctcaagatt atgatttggt
     4921 ccttcgtgtg acagaacaaa tccctgcaga tcatatttac cacattgacc gagtcttgta
     4981 tcattggcgg acgatcccag gatcaactgc aagtaatggc gaagccaaaa gttatatcta
     5041 tgattccgga gtgaaagcat tgacagatgc cctgtctaga agaaacatca aaggaagcgt
     5101 acgtcctggc agaatttctg gattttatga gattgcttat gacgtcttgc aagaggattt
     5161 ggtcagtgtg atcattccaa cgaagaatgg ctatgaagat ctaaaaactt gtgtggattc
     5221 gatcatcgaa aaaacgtctt attcaaatta tgaaatcatt attgcggata acggaagtac
     5281 agatccaaaa atgcaagaat tatttgcaga atacaagcat cagctgaaag accgctttat
     5341 cgtagaattg attgacattc cattcaatta ttctcgaatt aacaatttag cagcagaaaa
     5401 agcaaacggc aaatactttt tattcttgaa taacgataca gaagtgattg agccagattg
     5461 gatgacggtg atggtttctt atgcacagtt tgatcgaatc ggctgtgtag gagcaaaact
     5521 gttttatcca gatgatacaa cgcagcatgc aggagtatta ttaggaatcg gtggtgttgc
     5581 agggcatgca ttgaataact atgatcgaac acattgtggt tatttcggcc gattggtcat
     5641 tgacgtcaat tacttagcag tcactgcagc ttgtatgatg gtcaaggcag ctgacttcaa
     5701 tgctgttaac gggtttgatg aaacattaga agtagcattc aacgatgttg acttgtgctt
     5761 gaaagtttat gaactgggtc gttacaatgt ctatgcacat caagcagaat tgtaccattt
     5821 cgaatctaaa tcaagaggtt atgaagatac tccagaaaaa caaagacgtt ttgctggtga
     5881 aatcaaaaaa atgcaagata aatggccagc atatattgcg catgatccat tttacaatga
     5941 taatttaaca aaagacggaa tcggcgactt ttcgttgaga ccagattaaa ctataataaa
     6001 aagctgttat gttgtgattc gctcgcacat aacggctttt tatagttttt tgtcaaaaaa
     6061 aggcagaagg tgccgactca aaaaaataag tcgaggttgg gacagaagtg tttaactccg
     6121 agaaataaga agaaattcac gaaaattgct tttcaaattt ttgtgaattt cggcttattt
     6181 aagaagaagt tgcttctgct cccaccgttt attcgttttt aaagaatgag acaaaagtgt
     6241 ttcttacttt tgtcccacgc tcttattcat gctcagagcg ccaatagata caaaatcata
     6301 gccaaagttt cacgaaccct agctgtcaaa gaatcgatat tttattttaa tataggctat
     6361 tttaaaataa atggtttaag gtattcctct acaaaagcca ctctatcaat tatcatgggt
     6421 ttaaaataag aagtgatgga gataaccaga ttgttcttag cgtcaatata aagaacattt
     6481 ccactattac caattgctga atatatttcg ttctcagaat ctaataccca ccataaatag
     6541 ccatatctca tattttgtaa gtcttctaaa gcactggttc taggtttgaa catctcgttt
     6601 atccacatgg gtggtacgat ttgttttcct ttatacatcc ctttatttag gcataataca
     6661 ccaatttttg ccagatcact tgccgataaa cacaaaccat atcctgccgt cccgatattt
     6721 agtggatcac aaaaccagac attggtttta ggagacttat ccattgtaaa ctgcttatgt
     6781 tcttcagctg tttcagcata ataattgaca tggtttggta tccctatagg atcaaataga
     6841 tacttattgg caaaatcaac agttgttagc ttacttgtct gatacaacaa gccagttaat
     6901 atgtgtaaac agacagtttg atagttgaat tcgccggtta taccttttct tcctcccaat
     6961 aaatctagtg atgtttttgt ccaatcttca cttgaacaga cttttgtcca tggatcgcct
     7021 ttacatttgt agggtgcttt cattgttagc aagtgcttga ttgtaatagc gtaaatagtt
     7081 ttctccccgc gtttcacttt atactctgga aaataatcta atactttatc atcgatactt
     7141 ttgattaatc cttgctctaa agcaatgcca atcagcaaag atacaatact tttcgttgct
     7201 gacatgatat gacaagtatc gtctttttta taaccattcc attcatctgt ataaacttct
     7261 accccatcct tatatgcgac aatttgacat atatttggtt gtcgaaaacc aataaaatca
     7321 tgcaactctt gtttattcat aagatattac ctcactttct tctataagca taatgtcatt
     7381 aaaaatcgat ttcaattttt tcgattcttc acgtaataca ggtattctta tggactagac
     7441 agcgctttgg gaagatggat tctctgaaaa atataaaggt tcactttatg agtggataaa
     7501 aaaagcagca aatacttcga cttctttaat ttaccaacat tagtgaaaat cgattcatct
     7561 ggagatttgt aatttagagt ccttgtcttg attagaggtg tataaagctt tttattagac
     7621 atttttaaat tccattacaa tttgcttata aactaggttt ttaaaggtga ctatggtaag
     7681 ataatagaga tattattttg tgattaggag tgtatatatg aataaaaatg gagaatggag
     7741 tgctgcacga cggatattca ttatattaat ggatgttatt gtatacaatg cagcaatata
     7801 tagtagtttc ttactgaagt ttcgtggaga gatcccatta cgaaatttag aaactttcca
     7861 acactcagca attattattt cagtgatttt tattgcgttg aacattttgt tgggtgcata
     7921 tgttttttat aatcgaatga tcagtgacat cgtgtttgtc acagtcatcg gtcaggtatt
     7981 gatgacacta gggatcatgg tagtgacttt tgcgggccgc tggtttgctt ttccacgtct
     8041 tgttatctta ctttcatttg ttctcggtac gcttttctta agtatctatc gtattctcgt
     8101 atataaactt tatttacgcg taagtcgaga taaaaaggtc gtcattattg ggttagaaaa
     8161 ggatgtcgca ccagcaatcc aaaattttgg ttcaaagaaa aacaacaaac ataaagtcga
     8221 agcagtcatt cttgataact attatgcaaa catcaaaaat atgattaaag acatcgatat
     8281 tgtctatctt gcttctcata tcgaagaaac tgaaaaaatc aaaatctatc aattagttac
     8341 taaaaatgaa aaaaaattgt ttttgaatac aacatttgaa aacttgacat tgatcaatcc
     8401 aaatattatg aatttcgaag atgaaagtat tattgaagca tctggattta gaatccaacc
     8461 tgaatatgaa ctattcaaac gtttgtttga tatcatcgtt tcacttgttt tattagttat
     8521 tgcttcacca tttatgttat tcacagcaat tttagtcaaa gtgacatcac cgggaccaat
     8581 catctataaa caagtgcgga tcacaaaaaa tcaaaaggaa ttttcaatct ataaattccg
     8641 ctcgatgacc gccacagccg aagcaaaatc tggaccagtt ttagctaaga gcaatgatgc
     8701 acgagtaacg cctgtaggta aatttattcg agctgtccgc tttgatgaat taccacaaat
     8761 cttcaatgtg ctgaaaggcg atatgtcgat cgtaggacca cgtcctgaac gttctttctt
     8821 cgtcgatcag ttcaatgcag aaaaccctta ttactactta cggcataatg ttcgtgctgg
     8881 tatcactggg tatgcacaag tctatggaaa atatgtctct gattacaaca gtaaattacg
     8941 ttttgattta ctttatatca aaaaatattc attgatgctt gatttaaaga tcttgctgca
     9001 aaccatcaag atcctatttg ataaaatgtc ctctcaagga cttgaagaag acgaaaatgt
     9061 gatctcattg gcagaaattg cttttccgaa tgaaaaaatc tttcgctagt tgggaggcgt
     9121 aaaaatgaac aatccgaaat tcagcataat cgtaccagta tacaatgcag aagcaactat
     9181 tggtcgaacg attgatattc taaaagaatt ggattatgaa gagtacgaag tcgttttagt
     9241 aaatgatgga tcaacagatc aaacacagga aatcattcaa gaactgattg aaaatgatga
     9301 acgttttgaa ttgattacta cgaccaatca agggcccgga ttagcaagaa acgaaggaat
     9361 aagacagtca aaaggagaat atctcttatt cttcgatgca gatgacacgc cagtaaaaac
     9421 gatcttgtca gattatgatc atttgctaga aaagagtcca aaagcggatc ttgttgttag
     9481 ttcgtttgtt tttcgtacaa tggacgaggg aagaatcgtt tccgaaaaag aaaacttagt
     9541 tacagaatgt cactatctaa ctcacgaaga gtttataaaa gacatgtatc agttaatgaa
     9601 tgaacagttg atgtatgttg tatggaacaa atgctatcgt agcgatattc tgaaaaataa
     9661 ccaaattcta tttaaaactt ataatagttg tgaggatcga atatttaact tacattatta
     9721 tgaatactgt catcaagtga tcatgaaccc aaaagtagaa tatatttatg aatttgaagg
     9781 tgggaagggg atcactaatc aataccgacc taacaagttc gaaacattta aagaattcta
     9841 ttcattagca aatactgtaa cagacgatat taataaagac ggtatggcgg cgcttctttt
     9901 aaaagggaca acttcagtta ttttttctat ttatagtacc tctgcaagaa cttcaaaaga
     9961 gaagaaagac gaagcaaagc agatattgag tgatccagtg atccgagaag ccaagcaaat
    10021 tgctaagacg gattcttcag caaaaaaaat aacaaagttg ctttacaata tgccagctcc
    10081 actattttta tctgctgtaa aagttggaag cttcgttgaa gtgaaaatgc cagggttaat
    10141 ggctgctttg aagcgagtat attagttaat ggaggaaaat ataatgaaaa gaattttgca
    10201 ttttcaggga cgaatgggtt taggtggagc agagtctttc atgatgaatt tatatcgtaa
    10261 aattgatcgt acaaactatc aatttgattt tttgatttat gaagattatg cagatgttca
    10321 agactatcat tcagagattg aaagattagg aggccgtatt tttgttgtac ctaatcctaa
    10381 gaaaaatatt tttaaatatt taatagaagt gaataagtta ctaaaaaaag aatctttttc
    10441 tatagttcac aatcaggttt attttggcgg gggaattaat ttatggttag ccaaaaagaa
    10501 tggaatcaga cagagaattg ctcatagcca tgcaactgaa gatggaaaat ctcaaaatat
    10561 tgtaatgaat gtattgcgta aatttttaac caaattgttg ttacaaaatg caacagacta
    10621 ccttgccgtt agtcaagaag ctggtgaaag tctgtttcaa aaccatccct ttgagatagt
    10681 tcataatggt attgatttag agctttatag taaaaattca gaagctaagg ttaataagag
    10741 aaaagaattg gatatctcaa tgagtacttt tgttgtggga aacattggac gattagagaa
    10801 acaaaaaaat caatcttatt tgttagagat ttttttagaa atactaaaaa tgaattctga
    10861 ttcttacttg attttagtag gtgaagggtc acttagaaat aaactgaaag ccaaagcagt
    10921 agaattggat atagagaaaa gagtaaaatt tttaggagaa agaaacgata ttcccgagtt
    10981 attatctgtg atggatgttt ttgtaatgcc ttctctttat gaaggtctgc caatggttgg
    11041 attagaagca caagcagcaa aattaaaatt agtcttagca gatactattt ccaaagatac
    11101 aaagttaaca aataatgtgg attttgtatc attagatgaa acaccacaag aatgggcaaa
    11161 aaaagtaata aaagagccgt atgctaataa aataacacaa gaactaaaga gttatgatgt
    11221 ttcgtacact actgaacaaa tggagagtat atatagtaga gatcattaaa aatgttcaag
    11281 atattttagg agtgattatt tttgaaaata tgttatttga tattggcata caatgatatt
    11341 ggcaacttaa agagattggt caatcgattg aacgaaaatg cagtttttat tattcatata
    11401 gataaaaaaa ctgatataga accatttatt gaagcattta aatcatatga taatgttgaa
    11461 tttttatgta ataaaaaccg tgttaaaatt tattggggtg gtttttccat tgtacaagca
    11521 gaaatcaatt tagtaaagag agcattacaa aatgagaaat atttaaaata tgtattatta
    11581 tctggtgctg attatcca
//
LOCUS       DARBEC010000086        11591 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.089, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000086 DARBEC010000000
VERSION     DARBEC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11591
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.089"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(176..661)
                     /locus_tag="SCL80_002258"
     CDS             complement(176..661)
                     /locus_tag="SCL80_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEG1108105.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    751..1096
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1141..1782
                     /locus_tag="SCL80_002259"
     CDS             1141..1782
                     /locus_tag="SCL80_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEG1108106.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIIMKQVEGKLNYYR"
     gene            1801..2436
                     /locus_tag="SCL80_002260"
     CDS             1801..2436
                     /locus_tag="SCL80_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEG1108107.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            2454..3269
                     /locus_tag="SCL80_002261"
     CDS             2454..3269
                     /locus_tag="SCL80_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEG1108108.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            3588..5024
                     /locus_tag="SCL80_002262"
     CDS             3588..5024
                     /locus_tag="SCL80_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEG1108109.1"
                     /translation="MEHKELTSFPKDFLWGSASAAYQVEGAWQEDGKGESVWDRFVRI
                     PGKTFKGTNGDLAVDHYHRYKEDIALMKEQGLKAYRFSVAWTRIFPNGRGEVNQAGLA
                     FYERLIDELIENDIEPMLTLYHWDLPQALQDEYNGWESRQIIEDFTHYAETLFEAFRG
                     KVHYWISLNEQNIFTSLGYLLAAHPPGVTDPKRMYEVNHIANLANASVINKFHELEMP
                     GKIGPSFAYTPNYPIDSNPENVLAAENAEDLMANYWMDVYMWGKYPIAAMRFLEEKGW
                     APTIEAGDAELLESAKPDFLGINYYQTATNAFNPLNGVGAGKMNTTGKKGSSEETGVP
                     GMYKKVENPFVERTNWDWEIDPEGLRIGLRRITSRYRMPVLITENGLGEYDKLTEDKQ
                     IHDDYRINYLQSHVKAIKEAISDGAEVLGYCTWSYTDLLSWLNGYQKRYGFVYVDQDE
                     TQEGSLERYKKDSYYWYQKLIEENGENL"
     gene            5213..7660
                     /locus_tag="SCL80_002263"
     CDS             5213..7660
                     /locus_tag="SCL80_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1108110.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            7890..8957
                     /gene="rlmN"
                     /locus_tag="SCL80_002264"
     CDS             7890..8957
                     /gene="rlmN"
                     /locus_tag="SCL80_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1108111.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(9150..10253)
                     /locus_tag="SCL80_002265"
     CDS             complement(9150..10253)
                     /locus_tag="SCL80_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1108112.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            10806..11354
                     /gene="aac(6')"
                     /locus_tag="SCL80_002266"
     CDS             10806..11354
                     /gene="aac(6')"
                     /locus_tag="SCL80_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1108113.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         3846 a         1955 c         2503 g         3287 t
ORIGIN      
        1 ttttcgtaga atcaataaat atggatttgg ctaactatgg aagaatactc gtattagttt
       61 tcagcgttca tactagttgt tacattgttt gattggccaa aagaatttct gatatatttt
      121 gtgctaaata aagaaaaaga cgcttgaatt gggaaataag aaaactactc tatcattaca
      181 aattttcata aactggcaga tgttctaagt aaacaagtat cttttcaaag tcggaaaaat
      241 ccataatcaa cgtagtggta ttgatatttg gatgaaagcc tacggtatct tgacgatcaa
      301 tagcagcatc gatcacgact tggatttttt tctctttgtc gtgcatcaat gctaatggag
      361 tcactgtacc tgctggaacg cctaggaggt caggaagctg ttcttcagaa acaaaagata
      421 agcgcttttc ttttaattgt tcagctagtt gttttaaatc ggcgtgcttt tcatcaggca
      481 agatgacaag atagatttgt tttccttttc ttgatttcaa taatagattt tttacttgcg
      541 gaccaggtag ggaaaaaggg acatttttga ctgacgtaat cgcaggatga tcgacttttt
      601 gaaaagagat acccagtttt tttagtagtt cataagcttc ttgttcggtt gcacgactca
      661 tcgcgcatca ccctcctttt tttattagtg tatcataaaa aagagttgac aaatgaaaaa
      721 taatcatgta aattacacgt aaagttaaaa caaatgacat cgaaaagaag agtagccgtt
      781 aggtgttttt agagagtctc tggttggtgg aaagaggcag cagttaaccg tgaaacacat
      841 cttggagtcg attttctgaa gcgtatagta ggaaaatccg gggagcccgt tacagctgcg
      901 gtcgttgcta gatgaccaga taaggctggc ttttccgtga ggagtcggta aaaaaaggtg
      961 gaaccacgtg attaacgtcc tttgatcttc ggatcaaagg actttttgtt ttcatctgaa
     1021 cggcaacgac tgtgtgaggt tttttctgca aggaaaaaat aaacagaggt ggaaccacgt
     1081 ataatacgtc ctcttggcat ttgccaagag gattttttta ttttaaagga ggaaaagaag
     1141 atggagtatt tgattgaagt tatgccgcaa ttattagtag gagcactcac aacattgaaa
     1201 ttatttttat ttaccttgat tggctcatta ccattaggga ttgttttagc attaggattg
     1261 tctttgcatt ttaagccgct acgctacttg cttcaaacat atgtatgggt tatgcgggga
     1321 acaccgctgt tgttgcaact gatttttatt ttttatgggt tgccgacgat tgggatcgtt
     1381 tttgatcgtt tcgaagcagc actcattgcc tttatcttaa actatgcagc ttattttgca
     1441 gagatctttc gtggagggat acaatcgatt cctcaaggtc aatatgaagc agcgcaagtt
     1501 ttgcaattaa gtccctttca aacggtgacg aaaatcgtca tcccacaagt gacaaagatt
     1561 gttttacctt ctgtagggaa tgaagttatc aacttggtca aagattcttc cttgatctat
     1621 atattaggtt taggtgacgt aatgcgtgct gggaaaatcg cgatggaacg cgacgcgact
     1681 ttactgcctt tgctaggcgt ggcaatcatc tatcttacat tgactggtat tctaacaatc
     1741 ataatgaaac aagttgaagg aaaattaaat tattaccgct aactgatagg aggaagaaat
     1801 atgattgaat tgaagaaagt gaacaaagca tttggtgaaa aaaaaataat caaagatttg
     1861 aatctaatca ttccagatgg gaaaacatta gctatcgtag gtccttccgg tggcggtaaa
     1921 accacgcttt tacgtatctt ggctggctta gaaagttctg acagcggaga attttattta
     1981 gacggtgtac cgttcaaccc attcgagctt aaaatgaaag aacaagttgt aggagtcgta
     2041 ttccaagact tccagctatt tccacatctg aatatttttg aaaacattac cttagcacca
     2101 aaaatggtat tgaaacagac aaaagaagaa tacaccaaaa aagcagaaga attagcggct
     2161 tcattaggga ttagtgattt attgaatatc tatccttatc agctttctgg cggacaaaaa
     2221 cagcgggtag cgattgccag agcattggct atgaatccac gggtattggc ttatgatgaa
     2281 ccaacaagtg ctttagatcc tgaattacgc cagcaagtcg agcgtttgat cctatcattg
     2341 aaagaagatg gggtaaccca aattattgtc acccatgatt tagtatttgc agaaaatgtc
     2401 ggtgatcaat tactaaaagt ttccccaatt cattaagaaa ggagaagata gagatgaaaa
     2461 aaaccagttt tctattcgct ttgatcgcag gtcttcttct ttttgcaggc tgttcgaaca
     2521 aaaaaacttc agcagataat tgggcgacta tcaacgaaga gaaaaaaatc gttataggtt
     2581 tagacgatac atttgttcct atgggattta gagataaaga cgggaagctc acaggatttg
     2641 atattgattt agccaaagca gtctttgaac aatatgggat tacggtagac tttcagccca
     2701 ttgactggtc aatgaaggaa tttgaactga acaatggaac gatcgatttg atatggaacg
     2761 gctattccaa aacgccggca agagaaaaga aagtgcaatt tacaaaacct tatatggaaa
     2821 acgatcaagt gctgatcaca cctaaagaat caggaatcac aaactttgga caaatgaata
     2881 ataagagatt aggtgcgcaa aatgggtcat ctggttatga tgtatttacg aaacaaccag
     2941 aagtattaaa agagatagtc aaaaaccaag atgctgtttt gtatgatagt tttaatgaag
     3001 cattgatcga tctgaaaagc agacgggtcg atggtctgct gatggataaa gtatacgcag
     3061 actattattt gaaacaaaga aacgaactag aagacttcaa cataacaaaa ggttcttatg
     3121 agagtgagga ttttgcagtt ggtgcacgca agtcagatag tacattagtg aaaaaaatca
     3181 atgaagccct agatcaacta gagaaaacag gtgaattcca aaaaatatcc gataaatggt
     3241 ttggcgaaga tgttacgccg agaaattaac aaatagggga ctgtgacaaa agacgtgtca
     3301 cagtcccttt ttccgaataa acagtgttct cctgccaact ccttgtgact cggagctgaa
     3361 tggcaggctc acaacctctt ggtaaacggt gttcaaaagc cagtttttcg gtattaagcc
     3421 aaatttatgc aaaaatgtag aaagccattt tcacaaaatt tttcttacta ctcgaaacta
     3481 agcgactttt tcacaacctc ctttacataa ttttattttg ttgtggaagg ggtttcgtgt
     3541 tattctaaaa gaggaacgat tgaaggttat aggaaaggaa gagatgaatg gaacacaaag
     3601 aacttacctc atttccaaaa gattttctgt ggggctctgc ttctgcagcg tatcaagtag
     3661 aaggagcttg gcaagaagat gggaaaggag aatcagtctg ggatcgattt gtacggatac
     3721 ctggaaagac atttaaagga acgaacggtg atttggcagt agaccactat catcgttata
     3781 aagaagatat tgctttaatg aaagaacaag gtctgaaagc ttaccgcttc tctgtagctt
     3841 ggacacggat ttttcctaat ggacgaggag aagtcaatca agctgggctc gctttttatg
     3901 agagattgat cgacgaattg attgaaaacg atattgaacc tatgttgact ttatatcatt
     3961 gggatcttcc acaagcattg caagatgaat ataatggttg ggaatcacgt cagatcattg
     4021 aagatttcac tcattatgca gaaacattat ttgaggcatt ccgcgggaaa gtccattact
     4081 ggattagttt gaatgaacaa aatatcttca cttcattagg ttatttgtta gcagcacatc
     4141 cgccaggtgt gacagacccg aaacgaatgt atgaagttaa ccatattgct aatttagcaa
     4201 atgcatctgt catcaataaa tttcacgagt tagaaatgcc tggaaaaatc ggaccaagct
     4261 ttgcgtatac gccaaattat ccaattgaca gtaatcctga aaatgttctg gctgctgaaa
     4321 atgcagaaga cctgatggcc aattattgga tggatgtcta tatgtgggga aaatatccga
     4381 ttgctgccat gaggttctta gaagaaaaag gatgggcacc aacgattgaa gctggcgatg
     4441 cagaattgct agaatctgct aaaccagatt ttcttgggat caattactat caaacggcga
     4501 cgaatgcatt caatccatta aacggagttg gtgctggaaa aatgaatacg acaggaaaaa
     4561 aaggttcatc agaagaaact ggagttcctg gaatgtataa aaaagttgag aatccatttg
     4621 tcgaacgaac aaattgggac tgggagattg acccagaagg attaaggatc ggactgcgtc
     4681 gtatcacaag ccgttaccgc atgccggtac tgattacgga aaatggctta ggagagtatg
     4741 acaagttgac agaagataaa caaatccatg acgactatcg aatcaattac ttacaaagcc
     4801 acgtcaaagc catcaaagaa gcaatttcgg atggagcaga agtcctaggc tattgcacct
     4861 ggtcttatac agatttgctt agctggctga acggttatca aaagcgatat ggatttgtat
     4921 atgttgatca agacgaaaca caagaaggat ctttggaacg atataagaaa gacagttatt
     4981 actggtacca aaaactgatt gaagaaaatg gagagaacct ttagttcaca tacttaaaac
     5041 aagaaggtac ggctggatat ggccgtgcct tttcttgata aaatcattta gaaattaaga
     5101 aaaactgtaa aataagctga aaaatgaaag aaagcgtcag ttctttcaaa caaaacttta
     5161 gttcccttac ttttttttgt ataatgaaac ttgatgaaat gagggataat aaatggcaca
     5221 aaagaaacgg cctactaaaa aaaggaaaaa gacgaaaaaa acgaaaaagc aaaaacagca
     5281 gcaggaacaa atgctgacga ttggtgttgg gtttgccttg attttatttg ctgtcttcgg
     5341 ctttttaaaa ttaggttttt taggaatatt gattgcaaac ggctttcgga tcattgcagg
     5401 aaatacttac caaatcctct gtcttttatt agccgtatta ggattttgga ttgtcattaa
     5461 gaatacagag ttttcaattg gtaaaaaccg acgctggttt ggcggcatac tcttttattt
     5521 cggtatctta ttattgttac atgcccattt atttggcaaa ctccatactg gggaaccaaa
     5581 tatcatgggg acgacatggg atttgttagc cagtgatata aaacaaagtc aagtagacaa
     5641 taacgttggt ggtgggatga ttggagcaat cctttatcat tttacttact tcttgattgc
     5701 acagcctgga agttatttag cagcagtgct attactggca ggaggagcat tcctgttcag
     5761 taatctggaa ggctatcagc tgttaaatgg tatccaatca attggagaac gagtacagga
     5821 attattggaa ggtgatcctg ccaagcaagc tcgaaagcaa gcggcaaagg aagagagaat
     5881 gaaacagcgc gcagaagcga aagaagcaag gcgcttagct gcaaaagaag ctgcagaaaa
     5941 agaagctgca gaatatgaga agaaaaaagc ggtgagccag caaagggaaa atactgctga
     6001 tgaatggcag gaaccagaaa atacagagcc ggagcagcta agttttgttc caatcgacag
     6061 ttttcaagaa aatatccatc ctgcgaatct agagaaacca gtaccagata caccaaaaca
     6121 gaccaataca gcagaaggtt tttcagatga gttgccagag gatgacggta cctcattaga
     6181 atttgagatt gaagcagaac aggaaaatca agactatgaa ttaccgacag ttgatttgct
     6241 tgattctata ccaactgtgg atcaaagtga tgagtataaa aaaattgaaa aaaatatcgg
     6301 cgtattagaa caaactttcc aaagtttcgg tgtcgatgcc aaggtcgtca aagccagctt
     6361 aggaccatcc gtcacaaaat tcgaagtcca gccggctgta ggggtaaaag taagtaaaat
     6421 tgtgaactta acagatgata tcgcgcttgc tttagcggcg aaagatgtcc ggatggaagc
     6481 accgattcca ggtaaatcac tgattggtat tgaagtacca aatggaaaga tcagtatggt
     6541 ttcctttaga gaaatcatcg aagcacaacc aaaccaccca gataaattac tagaagtacc
     6601 tttaggacgt gatgtttctg gacgggtaca aacggctgat ctttcgaaga tgcctcactt
     6661 actcgtggct ggttcgactg gtagtgggaa gtcagtagca attaatggga ttatcacaag
     6721 tatattaatg cgtgctaagc cccatgaagt aaaactgatg atgatcgatc cgaaaatggt
     6781 tgaattgaat atgtacaacg gcatacctca tctgttgacg ccggtcgtga cgaaccctag
     6841 aaaagcagcg caagccttac aaaaagtagt acaagaaatg gaagaacgat atgaaaaatt
     6901 cgcggcaacc ggagtaagaa atatttctgg ttacaatgaa tttgttcaac agaaaaactt
     6961 ggaaaacgga acgaaacatc caaccttacc attcatcgta gtgatcgtag atgaattagc
     7021 tgatttgatg atggtagcca gcaatgaagt tgaagatgcg attattcgtt tagcgcaaat
     7081 ggctcgggca gcggggattc atatgatcct agctactcaa cgtccaagtg tggatgttat
     7141 cacaggaatc atcaaagcaa atgtaccttc cagaatggca tttgcggtat caagcggtac
     7201 agattctcgg acgatcattg attctaatgg ggcagaaaag ctcttagggc gaggagatat
     7261 gctattcttg ccaatgggtg agaataaacc gatccgtgtg caaggagcat tcatttctga
     7321 ccacgaagta gaacgggtcg ttcaatttgt tacagatcag caagaagctc attatgaaga
     7381 gaaaatgatg ccgacagatg aagttgaaac gacaggtgct ccagaacagc cacaagatga
     7441 gctgtttgaa gaagcaaaag cacttgtcgt tgaaatgcaa acggcaagta tttcccttct
     7501 ccaaagaaga tttcggatcg gttacaaccg agcagcacgt ttagtagatg aactggaagc
     7561 tcatggagtt gttggcccgt cagaaggaag caagcctcgg aaagtattca ttgagcagga
     7621 agaaacatca gaaacgttgc cggacgataa tgacatgtaa aacttgtgtg atgaagggtt
     7681 gaaaaacgaa taaccaataa aaagacaagg catgatgata ccaaaagaaa aaatggtttt
     7741 tttccatctt ttctaatacg gtatgatgtc ttgtcttttt agtttttgtg agattaaagt
     7801 tctattcgat ttatttgtga agtattgcat taaggaaatg aatcatgtat agtatgtagc
     7861 gaaactttga gaaaaagagg gaaaatactt tggaaaaaca gtccatttat ggcttgacga
     7921 atgaagaatt aatcaattgg tttatagaaa acggcgagaa aaaattccga gctgcccaag
     7981 tttgggaatg gttgtatcaa aaacgtgtaa gtaatttcac agaaatgacg aatctatcga
     8041 aacaattgat cgaaaaatta tcggagcatt ttatcatcaa tcctctaaaa cagatggtcg
     8101 tccaagaagc ttcggatggt acagtaaaat atttgtttga actccctgat aaaaacatga
     8161 ttgagactgt actgatgaga caagaatatg gcttgtcagt ttgtgtgacg acacaagtag
     8221 gatgtaatat cggctgtacg ttttgtgcaa gcgggctgct caaaaagaac cgtgatctga
     8281 ctgctggaga aattgttgca cagatcatga tggtccaaca ttattttgat gagcgcaatc
     8341 ttggcgaacg cgtttcacat gtcgttgtaa tggggatcgg tgagccattt gataattacg
     8401 acaatgtaat ggactttttg catatcatca atgatgccaa aggattggca attggtgctc
     8461 ggcacattac agtttccact agtggattag cacacaaaat caaggaattc gctaataacg
     8521 gcttgcaagt caatctggct atttccttgc acgcgccgaa taatgaggtg cgaacatcga
     8581 tcatgcggat caaccgaagt ttcccaattg agaaattgat ggaagctgtg gatgagtatt
     8641 tagagaaaac aaaccgtcga atcacgtttg aatatatcat gttgaatcaa gtaaatgata
     8701 gaccagagca tgcgcaacaa ttagcagatc tattaaaaga taagaaaaaa ttagcatatg
     8761 tcaacttgat tccatataat ccagtcagtg aacatgatca atattctcgc agtccaaaag
     8821 ctgatgtatt gagattttat gatgtattga agaaaaacgg cgtaaactgt gtgatacgta
     8881 aagaacatgg tacagatatt gatgcagctt gcggacaatt gcgaagcaag caaatgaaaa
     8941 aaacagttgc caaataatcg tatcctactc gttttttcaa ttaccataaa aggagcagtt
     9001 ttcttaatta aggaactgtt caacttaaat aagagaaaag attctatagc gaaagctata
     9061 aagctttttt tgacaagaaa aaccctcatc aagcaaagaa atgtcctcaa tttgatgaca
     9121 gcctgcttga tgggggtttt agcaattatt taactgattt tcttatatcc ttgctggctt
     9181 tcttgtgtag catgataagt ttctacgtca agaatggcct gtttaaattt ttctcgggtg
     9241 atttcataag gcacatgttt aatatcattt gttgccatgg ctttatctaa gattgcttct
     9301 aaatcggtcg tgacattgag attcatgtcg gttaacgata ctggaagttg gttctttttc
     9361 ataaacacat agattttatc aaacaaatcg aactgctgat ccatcatcaa taaggtcagt
     9421 acaccataac tgacagatgc tccgtgaagg tatccctcca ttttttctag aacagtcgta
     9481 ccgtaataga atgagtgtgc catagcagaa ttatagttat tgttaactaa aacagaaacc
     9541 aatccggtag taacaatgat atccgaaacg acttgttcaa ttgcatatga gggacgatcg
     9601 tcggcactat ctttcaatgc ttgcgtacca taagtcaaga gacgatcaat gcacattttt
     9661 ccgatttgga ttcctaatct atccgtgtag cctagatcat cgccacgagc agaaaaagta
     9721 gattcgactt ctttggataa agcatcgcca atacctgccc ataagtattt atcaggtgct
     9781 tcggcaataa ttgctgtatc aatgaaggtg tgtacaggtg gttttgaagg aaagaagagt
     9841 ccgtccatta cgccttcttc gttatagatg acacataccg cagtgacggg cgaacagttc
     9901 gaaccaatag tgggaaaggt gaagactggt ttagctagtt tttcaccaat gacttttaca
     9961 gtatcgcaag cacggccacc accgactgca aacaggacgt ctgctttttg gacactttca
    10021 tgctggatca actgttcaat atttccataa gttgaattac ccccgtacca gagactggcg
    10081 acaacctcta tttcctgtgt caaagctact tttattttgt tttctgcctt tgaaagtgca
    10141 gtcttccctc caatcagcac tacttttgtc ccatattctt tgacgattcc atttgctttt
    10201 tcatacgcat ctgttccaat ggtataattt ggtaagaaaa cactttgact catattcatt
    10261 tccttctttc ttcgttgaaa cgggttaaaa ttttaaagag tgatcgatct gactgcggct
    10321 gtagatggag tagaggtgaa cctaagacag gataagtgat gaataaggtg gcatgaacac
    10381 cttgaaatca tcaataagta tcctaaaaac gaacaagtac aaaaacattg aaaacgacaa
    10441 taatgaattt ttgttcctct tagtatttct accgattccc tttgcttttt tgtaattatc
    10501 taaaaattct aaaaattaag attattatat gaaaacaggt tttatttgtc aattcataat
    10561 tggttttttt taaagcaaaa gataatctat atcatgatga acaaacagag ttttttcctc
    10621 caattgatgg ctttcctgat ctttcctatt ttgaataaag tgtttctgta taattttttt
    10681 attttataag taagataaat aatgaaagaa tagagatact gattggtagc taaagatttt
    10741 tgagcgagaa catttcgttt tttcaaaagg actgttagaa taagcaagaa caagggggaa
    10801 gacgtatgat aatcagtgaa tttgaccgta ataatccagt attgaaagat cagctttctg
    10861 atttactgag actgacttgg ccggaagaat atggagacag ctcggcagaa gaagtagaag
    10921 aaatgatgaa tccagaacga atcgcggtag cagcggtaga ccaagatgag ttagtaggat
    10981 ttattggtgc aatccctcaa tacggtatca caggttggga attgcatcca ttagttgtag
    11041 aaagctcccg acgaaagaac caaataggta ctcgattagt caattactta gaaaaagaag
    11101 tagcttccag aggaggaatc acgatttatt taggtacgga tgatttagac catggaacaa
    11161 cgttaagtca aacggacctg tatgagcata catttgataa agtggcttcc atccagaacc
    11221 ttcgtgaaca tccgtatgaa ttctatgaaa aattaggtta taaaatcgta ggtgtcttac
    11281 caaatgcaaa tggctgggac aaaccggata tttggatggc aaaaacgatt attcctcgac
    11341 caaattctca ataataaaaa gaggttgtga cagaagtaat cagcttcaag aaataagaag
    11401 gaatttacga aaattacttc ttgaattttt gtgaaattcc aacttatttc cgaagaagct
    11461 acttttggaa caccgtgtac cagaggttgt gaaaaagtcg cttagtttcg agtagtaaga
    11521 aaaattttgt gaaaatggct ttctacattt ttgcataaat ttggcttaat accgagaaac
    11581 tggcttttga a
//
LOCUS       DARBEC010000087        11566 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.090, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000087 DARBEC010000000
VERSION     DARBEC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.090"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            374..868
                     /locus_tag="SCL80_002267"
     CDS             374..868
                     /locus_tag="SCL80_002267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108114.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQAA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLSEAQARGLTPCSK
                     CYGN"
     gene            complement(1030..2202)
                     /locus_tag="SCL80_002268"
     CDS             complement(1030..2202)
                     /locus_tag="SCL80_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1108115.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEGSSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(2408..3223)
                     /locus_tag="SCL80_002269"
     CDS             complement(2408..3223)
                     /locus_tag="SCL80_002269"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1108116.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(3373..3819)
                     /locus_tag="SCL80_002270"
     CDS             complement(3373..3819)
                     /locus_tag="SCL80_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1108117.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(3816..4820)
                     /locus_tag="SCL80_002271"
     CDS             complement(3816..4820)
                     /locus_tag="SCL80_002271"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEG1108118.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(4860..5189)
                     /locus_tag="SCL80_002272"
     CDS             complement(4860..5189)
                     /locus_tag="SCL80_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEG1108119.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(5315..7642)
                     /locus_tag="SCL80_002273"
     CDS             complement(5315..7642)
                     /locus_tag="SCL80_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1108120.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
     gene            complement(8485..8778)
                     /locus_tag="SCL80_002274"
     CDS             complement(8485..8778)
                     /locus_tag="SCL80_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEG1108121.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(9181..10359)
                     /locus_tag="SCL80_002275"
     CDS             complement(9181..10359)
                     /locus_tag="SCL80_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1108122.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(10463..10777)
                     /locus_tag="SCL80_002276"
     CDS             complement(10463..10777)
                     /locus_tag="SCL80_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108123.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            10884..11102
                     /locus_tag="SCL80_002277"
     CDS             10884..11102
                     /locus_tag="SCL80_002277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108124.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWKS"
     gene            complement(11097..11492)
                     /locus_tag="SCL80_002278"
                     /pseudo
     CDS             complement(11097..11492)
                     /locus_tag="SCL80_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT         3349 a         2263 c         2018 g         3936 t
ORIGIN      
        1 cttaataaca gaactatagc aaaaaatccc gtgtatcaac acgggattag attaagtttc
       61 ctcaatcact attcataaca caattattat aacaagtaag taaacaattg aaaaggacat
      121 ttattgaaaa cggttgtttt ttacttaagg aggaaattta gtgaaaaaat tagttgcgtt
      181 gtttttggtc gcaggaagtt tgttgagtgg gtgtggtgct actacgtcta ctactcaaag
      241 cacaagttca gctcaggctt ccagcagcag taccacgatc tccgaaagta cggcagattc
      301 atcgaaagaa caaattgaaa agaaaaaaga agaagagaaa caaaaagcgg aaaaagctgc
      361 actagaaaaa aaagtgacag aagcggaatc tgctttgtca caagctgaag caaatcctac
      421 aaaagaaaac tatgcaactg cttcagcagc tatccaagcg ctacctgaaa acaaacaaga
      481 gttgacaagc cgtcttgctg caattgattc cacaatcaaa gcaaaagaag cagaagaaga
      541 acggaaaaag caagaagaaa ttgcacgaca acaagcagaa caagcgcaac aagaagcaat
      601 cgcacagcaa caagcagaac aggcgaggca agaagcaatc gcacagcaac aagcagaaca
      661 ggcgcgacag caagcagcag ctgaacaggc ttctcagcag gcacaagccg cagctcctga
      721 gcaaaataat gaacaaactg tctatgtcac accgaccggt tcaaaatacc atacacataa
      781 atgtggaaat ggtacgtata cccctgccac tttaagcgaa gctcaagctc gtggtttaac
      841 cccttgctcc aaatgttatg gaaactaatt caaaagttat actataataa taaaattctt
      901 ctgaaacata aaaaaataga agctggcgtt ccagatagta aaccaaaaat agcagtttac
      961 acgtccattt cgtcagcttc tctctttttt catttagtct gttccctctg cttttttgga
     1021 catcttcatt cattctttct caatcaaata gtacacctct ttttcttttc gcagtttata
     1081 tcttcctttt ttcaatttca ttgaaaactg tttcgaatgt ttatgtttta catggatttt
     1141 aagcaggagc tcctcatgtt gcaagcgata gttcctgcct tttaaatcag aaaatctcac
     1201 tagtccgtat tcatcagaag ctttcacaaa tggaactttt tctctagtaa tatagtgttt
     1261 gccgttgagc gtttttacag aaaagaccat tccttcgatt ggtcgtccgt ttgtgttctt
     1321 tacataaaaa gataaaggat agtttcgctt agcgatggcc aaagaatcta aataagcgat
     1381 gagtagccaa actaagccga ttgatccaag tagtataccg ataaaccaaa taaaaaattt
     1441 ccattttttc cattgatcaa ttccttggat cgcttttttt gcctcttttg cttgataagg
     1501 aatacgatgc cccgtcacaa gcagtcgatg tgtattgacc atataaggag tgcaggtaag
     1561 caaagtcacg agatctctgc ctttttggat acccaaagaa tctacttcgt caggtaagac
     1621 cacttggatt ttttctactt gatacgccaa tgtctttcca ttgatctgga tgaaaaactg
     1681 gtctcctttt ttcaacttag gcaaatctgt aaataatttt gcttctggga gtccacgatg
     1741 cccagaaaga acggcatggg tgtttttccc gccagtcgga taagaacttc cttccaataa
     1801 tgacgttcct ttttgcaaaa atatttccgt tgtctgatcg aatataggta agctgacatt
     1861 gatttttggg atttcgatga ctccaagcat atgttccata aaaaaggcat tactaggtaa
     1921 atcttttaat gcttttgcat caactgcttg gttgaaactg cctatccctg gtgatacgtt
     1981 ttgctctgcc aactgttggt ttttctttgt cattttttcc tgctgttttt ttatttcctc
     2041 tgaattctta ttcgatgctt gcttttgata atggatgatc atttgctggg caagaaaatc
     2101 attcagtgaa tcttctacaa aaggataaga aaagatcccg atcccaacga ctaaaatgat
     2161 tgccatgaag atgtccagta ttcttattct gcttttattc atcatgtcca cctattttct
     2221 aatatgtata tgaagaggtt gtgaaatcag cgccttgacc tgagtattag cgaagcaaac
     2281 ggcaaaatag ttcctcctat tttgccgttt gttgagcgaa taccaagaga ccgaccactt
     2341 gtcacaacct ttctccccat ttgaatcact atcgtttatt tatcgtatga tttttgtttc
     2401 caatagttta agcttctgtt tcttgatggc gttttttgaa atagatcact gctcctccaa
     2461 ctaaagcagt acctaccaag atgaatacga tgatccccat accaccagtt gaaggcaaga
     2521 ttcctttagg ttggttatcg atcgttgttt catcagaagt tgttggatca tcatctactg
     2581 taaattcagt gtacgcagct tcattcttag gtaatgcgta gccttctggt gcttttgttt
     2641 cttgaaggca gtatgcccct tgtttcagcc cttcgattga aaatttccct tctgaatcag
     2701 aaatcaattt cacttcttcc cagtcagaac ctgtgacttc atctgaccaa gcatatgggt
     2761 attctcctga ggcaaggttt gctatttttt tcattaattt aacagttgga tcttttgctc
     2821 ttagaataga aaattctgca tcggctaatt tttcttgcgt gttcacatct tgtttgatga
     2881 agtggaaatc accgtagttt tggacatttt ctacagtaat cgtgttatcc gtagttgtat
     2941 tttcccgatt gactacaccg tttggattcc caagattggc ttcatctgcg ccatggatga
     3001 taccggttgt ttgttgcaat tttccgtttt ttactataaa agtagtttct ttttgtggat
     3061 tggttaattg ataaccatct ggtgcttttg tttcaactaa agtataattg ccgtctatta
     3121 atccattgac actgatggat ccttcagtat cagttgtgaa tgtcaacgct gactcagcag
     3181 gatcatagag agcgacacca gtgttttcat caaaaccggt cacgaattga ttctctgaat
     3241 ttttgatctg gaattgcgcc cctgctaact tatctacccc tgaaaagttc ccagctttct
     3301 ggattttgat attgctttct ttgatgacat ttttagcata taggtgaatc gtgtctaaag
     3361 gtttgtcagg gtctagggta agcttaacac gatatttggc gacgtttcga caccgggctt
     3421 tggtgtttct acaaagacgt atacagcatc tttcccatca gatttacctg gaagagtcag
     3481 gttagctaat ccttcaccag aagtcgttac acttgcgaag ggtgtagcgt ctgctggaac
     3541 tgctgtgtct ttacttggat agtacgtagc agtgatgtca tatacgttga attcaacgcc
     3601 gttcaacggt tcgctattcg gaaatgtcaa ttccgttccg ttgtttattt gattttctgg
     3661 tgctgaagtg aatttcaatt tatgcaaatt aaccgtaaca ttttctggtg taggtgatac
     3721 ggctgcagaa acctctgcag ttcctaggcc gagaacgaaa ggaagcagca tgatagcagt
     3781 tcctaaaaac gtccatattt ttgttttgat gtttttcatt ttatttccct cctattgttt
     3841 cctttttgtt gttttttata aaaccaagct gtccctgttg aaaggactag caataaccct
     3901 aaaattgaga aacctccaag catctcttcg cttgtttttg gaaagcttcc tccatttttt
     3961 tcatttgatg gaccatttgt ctgcatctga actggcgttt ttccattatt cgtcggattt
     4021 gcttttggtt cgttgatgat ttccaatggt ataggatgaa gcggatcata actgcctttt
     4081 tcaacttcaa agagaatggt tgattgactc aatatatatc ctttaggcgg tttaatctcc
     4141 actagttcgt aacttccaaa agacaatcca tggacagaaa atgttccatc tttactggaa
     4201 gtaaaaatcg ttgctcttga atcatctttg gctacccaat gatttatccc gttttcttgc
     4261 gctaagtatt ggtcattttt tttgactaga aattgagcat ctgctagttt ttgtttcgtt
     4321 tgccgatcca ctttgacaaa ttgtttgcct cctgtttgga tggcaagatg tgtttctact
     4381 gtttgattgt cagtcgtcaa acgtacatcg ttatcaaatg tcgttttttc actgttttct
     4441 ttcaatgtca tttggtaacg gatcgttaat ttttttccag caaacgaact aagatctttt
     4501 ccagaatctg ataagacaaa accatgtttt attttttgtg tgtgaaatgt atgaatcttg
     4561 tctccaccaa tcaaaaaatc gatcgtttct ggttctagcc ataactgagg atcagctgta
     4621 tctttgatcg tataggttcc tatctcgttg atgttctcag gaattgttac ggtgatgaga
     4681 taagggactt tatccccatc tgcaaaatct gtctgcttat tctctactgt ttttgttaat
     4741 gtgagtgcag caggtatttt ttgttcgctc tttggataca agtggatggt cgtcaattta
     4801 tgattagatg aatctgtcac aggaagcaca acgacaaatg gtgctgattt ttcttttact
     4861 taatctgaag tctttatttc cgtaaatcgg taaacagcgt cacgaccttg ctcatctttg
     4921 tccgatgcac taaaagaagc aatgccctct tcctcgtcta ctgtttttgt cacattttct
     4981 gccagtattt tttcactatc acttttttgt gctagttttc tttgtgcgtc ttccacacta
     5041 cttccttctg accgcagctt gtaaaattca tccgagacat catatatagc gaaagtaacg
     5101 ccgttcaacc cttttactcc ttgaaagtta tccgggatat ttattccgtc gttttgactg
     5161 atacttggca tcttatcgct ttcgaacatt cgattatgca aagtaaatgt aataggtgat
     5221 tctctagctg acacagtcgg agaacctata aaaaagacca gtaggataaa aaagagaaca
     5281 gcaaatatat ccacacgtac attttttgtt tttttcatcg ctgttcacct gcttttttct
     5341 tacgatggat aaaataaaag aagccagaaa aaataaagat cagtcctaaa aagacaaagg
     5401 gcagtagacc tatcccacct gtcgaaggaa gcaagagagt cggattgatc gtcaacttag
     5461 cggtatctgt tgcgataatc gtataattat tgtttacact tccttcgcct aaactaatcg
     5521 ttactatttg tgatccaata tctgtccggc tgttaactgt tagggtataa tccaatgatt
     5581 cgcctaagac aacaccggta acgtggagat ttccaaccga aatcgagtga ctttgggcat
     5641 cataattaaa ggcagcgtca tcgacagtaa tcactgcgtt agctttatag atggtataag
     5701 ttcctgatgt gttggctata tcagtaaatg acaacttggg atttgctgct ttgactttgt
     5761 ctattccgct attcgttagt ttaattgtat agctgccagc attaaccata ttttttgctg
     5821 attgtgcatc tgctgtgtaa tccatactgc taagtgccac ttgtactgga gtaagcgaac
     5881 tagtgacacc ctctgctagc ttgacatgga ttgacgggac acctgctttt ccatcatatg
     5941 tcttactgcc actgagctct accgcaccgc tgatttgctt aatcgtatag acatcagatg
     6001 tctgcacgat agtaaataaa tagtttgttc tggcagctag tttagctagt ccgctttttg
     6061 tcaattgcca agacgtatag gttccaattt cagtaggcgc agttacttca actccctttt
     6121 cattcatcgc tttgaaatca gttggattcc atgtgatttg atcaatactg agatcattgt
     6181 ttaaaaggga tgttgattct gttttatcat taaaggcata tttaacgatc ggtacaacag
     6241 aaagaccatc atagtactta ctgccatttg cattgattga tactgttgca tttgtcttat
     6301 aatataggat gacttcagaa atctgttttc catttgcgtc ctttacgatt acatttccat
     6361 ttgtatctaa actagctaaa acactcattg ctgattggtt ctttgtttct atcgctgaaa
     6421 cagctgtaaa ggtatatccg ttgatagtag gcgtattagt cgtcaggtct tgtgcagcac
     6481 ttccgtaagt aagattcatt attttagaag gagcaatcat ctgtccaaaa tagtcttgat
     6541 acttaatagt gattggtgtt tgtttgcctg tataggtcac cgtatcgacg ttactttcta
     6601 gggctgtgaa agaaccgttt gtttgtgcaa aagaatagcc cgttactgct gttggagtga
     6661 taaccacttt gtcacccaca tggattccag ataatgtagt cccgtacgtt ccgatttttg
     6721 ctgctccacc attgactgta taagaaaaag ttcctgtccg attcgcattg acctgaaccg
     6781 ttgctgcttg ataggttgga aaatttgaaa catttccctt accatcaacg atctgcccat
     6841 ttttgatcga gtaccccgtg aatggaatat catttgctaa ctgtataccg attgaaaaag
     6901 tactcatacc tcctccggca ggaagccctc cccatgttga gggtgtaaaa gaataacttt
     6961 tatccgcatt gggtgtaact gaatcgatcg tatttccagc ggtagccacc cccatataga
     7021 tactttttgg gaatgtgata gtagtaccat ttactgtgga gccaataaac agattatgga
     7081 tatctggacg gttatcaata aaagcgattg ctttggttac gccttgtaac gcagttttat
     7141 ccgcactcat cgatccgtta accgcaacaa tgactccatc tttggcatat atcactgtat
     7201 atccatctgg tatggtatgt ccgttgaaat tcgcagtggc cgcaacagta ctaaatgtct
     7261 ggcttctacc tgtttctgaa taagctttct cccctgctgc ttttgctgct gtgatttctt
     7321 tttctgtcgg atcaatcagt tgagtcgagg atgtggttga ggttgtctcg ttgttgctag
     7381 actgattggt gctttctatg gactgtattt cagaataggg tggactattt gtctcctctg
     7441 tcgtttggtt agcaaaattt gacgtttctg tataaacgtt tacataaata cttgataagc
     7501 tccctaagaa agtaaaaatc aagatgatcg aaatcataat gggatgtaag tattttcttt
     7561 tagatacagc tttgagtcta gaaacaaaat gtttttgaca tatacgttgg attttatttt
     7621 gagttttctt aatcattttc atccctcctc tcaataaaaa atcatcaaat aatcctttac
     7681 acgttattct aaatttaatt tttattattt tattgatgta tagaaagttt ggggcttttt
     7741 ttctcttttt attctaaggt tattttagtc ttgtccttta atacaaagat tttattttta
     7801 aataaaaaat actctttttt tacgattatt gaaaaaaaaa gataaaactt gattaattga
     7861 agtattgctt aaatttggaa gctatgaggg aagaataaaa gagttaggac gggattatct
     7921 actatttttc tttgttttaa atatttgtta tctttgaaca taaaagagaa atgtgtcgac
     7981 tgcagtgttg tgtccctcat cacgaataaa cggtgttcaa ttagctgatc ttgtattcac
     8041 ttaaaaaatg gtaaagtacg agttgctatt ccccattttc aattttctaa cgctcaggac
     8101 aaaatactgt tttcacaacc tcattggaat aaaagcatat atattgtttc aagaacgaaa
     8161 tcttgttatc tttttaaagg gcatctgtcc aaaatctaat tttaggaggt acgttttggt
     8221 atttattcgt acaaataaaa gagaattatc tttattcatg taacgcatcc aattgatttt
     8281 ttcatgtacg ctcctggaac gattttactg acgattgctt atttaactgc gaatcgctca
     8341 ttagctttta tcatagctat ccatattttg aataacgttc ttggctttgt attatagata
     8401 cagatgacaa aaaaagttca agtatgcggt gtagaaccaa tatcgcatac ttgaactttt
     8461 ttcaatgagt ataaattaaa acttctaatt ttcaattccc atcatctgat atgcccgacc
     8521 gtctgaactt cttttgaaaa agccaaatgt gattagataa cgtctttgtt cgacataatc
     8581 gccactagaa aatacgttca gcagtatcga attaacctct gtttctgtat aagtcactcc
     8641 atggtcaaag cgtttcaaga cttctttcaa taggatgtat ttcttttttg ttgaacttgg
     8701 tacttgtttt acttttccat cattcatgaa tgtgtctaaa actttcatct tttcttgctc
     8761 cgtataagta taaaccatct tctttctcct tttgattcta agaatactcc atttatgtta
     8821 gaatattctg gattttttat taaagcggtt actcttaaga aagatattat catagtgctc
     8881 ttttttttac tactacttat aggtagattt attctaatcc attattctcc cctaccaata
     8941 attaaagaaa aaactggatc agggtgttca aattacatgc taagaaaaaa gaacccttga
     9001 ataccaaggg ttctttctca gtctgataac aatgattaac gacggatttc tttgatactt
     9061 gtgaaaagtc gttaaagtat tgatataaaa ggatttttat tacctctgac tacatattga
     9121 ctaatttgtt aaaaaatcgt ttttcaagaa tcctgtaata ccagtgcttt caccgttttt
     9181 ttaactctct aaagaactaa aagcaatcag aggttttacc ttttcatttg acgttaagtg
     9241 gcgataaatt ttcgaagttg tttggtcagt atggcctaag cgatctttca agacttccca
     9301 gctacagtgt tccgctacta agcttgccat tgtatgacga gctaaatgag tatgaaatgg
     9361 cttattaatt cctgagcgat tcacgcaccg tttgacgtga tcatttacat actctcgacg
     9421 ataaggaaca ccacgtttac tagaatcaag atacacaaac aaatatgttt cttctataat
     9481 aaacctgtaa tgttgcctca tctcacccaa agccttaatc aactgttgta gcttttccat
     9541 aactagagga gttactagaa ctcgtctagc agaactaact gtcttagtag tttcaattcg
     9601 aaaatcctta ctcttacctt taactgccgt ttgcttatag aacagaattt catttctttt
     9661 gaaatctata tctccaggct gtagagcaag cgcctcgctg actcggcagc cagtgtaaaa
     9721 tagaacatgg aaaagtacag acatcggtaa ttcctcatga acaatgccat aattcaaaaa
     9781 agttctcaat tcatcgacag taagaaactt gtcctcaata ttatttatgg cttcttttaa
     9841 atcagttacc gttaactgat attttggcat tctcacagac ttcattggat tttcacttat
     9901 caaattcttt ctttgacaat atttaaaaat catatttaga acttgaataa gtgattgtaa
     9961 actatttttg gcatagccaa catttgttcc attaacgcca cattccttta aggtaaaaag
    10021 caattcttga aggcactctg gtgtcaaatc agacaacttt ttatcaccaa tccatcgaac
    10081 aataattttt aattgttcct ctcgcttatc ataggttatt tccttttgtt catttaacga
    10141 acggtaatat tggaaccatt gctcacctac aacactaaac ttgtcattgc ttgcaacaat
    10201 ttttttctta agcctttgtt gtttctccat ttccagataa gcgagttgcg cctcacgttt
    10261 tgaattaaag ccacgtcttg ttgttcgata tgcttgaaag taccatgtga tcctaccagt
    10321 agaactgatt tgcttcttga atcgatctgt tttttccatt tattgattcc tcctattttg
    10381 aaggaaccca acaagcagaa accctttgga aatctttcta tctatgtcat cattgtaccg
    10441 catcttagaa agaaaatgta acttataaag atcgaagtag tcgcaatttt tctcgatagt
    10501 cattcgaatc actctttctc atccattcta aaacatcatc acgaaaatag tgtcctcgaa
    10561 tcccgtacca acagggagga aactcaccaa gttttactaa acggttcagt gtactatcac
    10621 ttatctgaaa tagtcgcttt aaatcagtat ttgttaatac cagaggtaaa tgactgactg
    10681 caagcaattt ttccgtgtaa tcttccagtt ttttttgtaa aattgaatag aactctattt
    10741 ccagagaatt aacatttgtt ttaacttcca actccatcac ccttctctct caaaatcaaa
    10801 acgaccacca cggtacccct taaaattgta aaaaaacact ccagtactcc atcaaaacgg
    10861 actagaaaag aggaattttt aatatgaaac aaggaattgc agacataaaa ataattaaag
    10921 aaattcttga aaaatctacg gctaatgcaa tagcattcgg aacaggaatc aacttgagta
    10981 cagtaaaaaa acttaaaagt ggagagagag ctgaagaaaa attaaatctt gccgatgcta
    11041 ttaaaatcac agaatttggc atgaaaaata tgccaaccaa aatcgaaata tggaaatcct
    11101 agctcttcca aagccatttg tcgttggtac caaacactgc cgagactttt cccaataata
    11161 tcgccaattt cacgatcatt aagaccgatg aagaaggaca taaaaatgat ttcttgtctt
    11221 tgactaggta atgctgcaat cgcatttgcc aactcctcat tggaaataaa gattttcata
    11281 ccaaggacaa taaaagtacc actactattc aaatttggtt ccaaataata gaactctgta
    11341 cctaaccctt tttgttccaa tatttccatt gatatttgcc gttggcgaaa tctagcgtac
    11401 ttcttttgaa tatttaacgc ctcattgcag actactttct tacaaaaact gtcaaacgta
    11461 tgttgaatgt gggtttcctc tgagtcgccc ataatattct ccccctcaaa aaattcattc
    11521 aactcgtttt acttctatcc ctttccatta ttactacaat ctagga
//
LOCUS       DARBEC010000088        11532 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.091, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000088 DARBEC010000000
VERSION     DARBEC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.091"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..679)
                     /locus_tag="SCL80_002279"
     CDS             complement(134..679)
                     /locus_tag="SCL80_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEG1108125.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDAVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(691..1383)
                     /locus_tag="SCL80_002280"
     CDS             complement(691..1383)
                     /locus_tag="SCL80_002280"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEG1108126.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYKPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(1402..1686)
                     /locus_tag="SCL80_002281"
     CDS             complement(1402..1686)
                     /locus_tag="SCL80_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108127.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(1699..2262)
                     /locus_tag="SCL80_002282"
     CDS             complement(1699..2262)
                     /locus_tag="SCL80_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEG1108128.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            2490..3152
                     /locus_tag="SCL80_002283"
     CDS             2490..3152
                     /locus_tag="SCL80_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEG1108129.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            3276..4472
                     /locus_tag="SCL80_002284"
     CDS             3276..4472
                     /locus_tag="SCL80_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEG1108130.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(4573..5949)
                     /locus_tag="SCL80_002285"
     CDS             complement(4573..5949)
                     /locus_tag="SCL80_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108131.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(5986..6852)
                     /locus_tag="SCL80_002286"
     CDS             complement(5986..6852)
                     /locus_tag="SCL80_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEG1108132.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(6941..7966)
                     /locus_tag="SCL80_002287"
     CDS             complement(6941..7966)
                     /locus_tag="SCL80_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1108133.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(7984..8658)
                     /locus_tag="SCL80_002288"
     CDS             complement(7984..8658)
                     /locus_tag="SCL80_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108134.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(8842..9735)
                     /locus_tag="SCL80_002289"
     CDS             complement(8842..9735)
                     /locus_tag="SCL80_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEG1108135.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(9809..11506)
                     /locus_tag="SCL80_002290"
     CDS             complement(9809..11506)
                     /locus_tag="SCL80_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEG1108136.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
BASE COUNT         3426 a         2523 c         1976 g         3607 t
ORIGIN      
        1 agagatttat taacgtaaaa agaataagtt tttttaaaat aacgtataaa aaagagcata
       61 aaacaaagtc gtttgtttta tgctcttctt ttattatttt gtgcttttta ttttatcaaa
      121 tcactttgtt tttttataaa atttctgcgc ctagtgtgga ctcaaaatgt ctcaatgccc
      181 attcatgtcc aacgggatcg aaacttgcca cggcatcttt gaaaatatgg agtttgtatc
      241 ctagattata cgcatcgact gctgtatgca acacacagat atctgtacat acgccggtca
      301 aataaatatc tgtgatctgg cgttctcgca aacggatgtc tagatctgtt ccactaaaag
      361 cggaataatg ccgtttgtct atccaataaa cattttcctc ttgctcatag tcttggtata
      421 aactttttaa agagccgtac aattcccgtc cacccgttcc tatcacatta tgcggaggaa
      481 ataagcggtt ctccggatga aaggaatctt ttggatcatg cgcatcaatg gcaaaaacga
      541 caaaatcctt ttgatcaata aacttctttg tatgagcagt cagctttttt tcaattactt
      601 gtcctgctgt cccagtagtc agctttccat cctctgcaac aaaatcatat gtgtagtcaa
      661 tcgagatcaa tgcgttcatc ttagtcctcc ttatacgaga tgtttgacaa attcgatcac
      721 catttctgct gatcgtttgc ctgcgtcttc gataaattcg tcaaagcttt gtgtcgccga
      781 gtgatctgct gtgtcgctca ttgcacgtac gatcaaaaat gggatattga actgacgagc
      841 tgtttgtcca acagcggctc cttccatttc gcaagctaat gcttcaggga aattcgttaa
      901 gatttctttg attttatctg gcgagtcgac aaatgtatca cctgtaacga tcaatccttc
      961 ttttgcgttc aaattcgttg cttttgctgc tttaaccatt tcactgcgca aatatgtgct
     1021 tgcttcataa tatagaggca ttcctggtaa ttgtcctggt ttgtagccaa atcctgttgc
     1081 atctgcatca aaataagcta ctttgtctga tataacgatg tcacctactt gtaagccttc
     1141 accgattccg ccagctgaac ctgtattgat caccatgttc acgccgtatt gttggatcaa
     1201 cagacttgtg gtaatagatg ctaagacttt tcctataccg gaacgtacga cgatcacttc
     1261 gtggttacct aatgaacctg aaataaataa agcgcctgcg cgttcccatg ataatggttc
     1321 gcttaggttc tctctcaaaa tttttacttc ttcttccatt gcgccgataa taccgatttt
     1381 catcttttta attccttctt tctatataaa tgcgattgct aaaaaaacca cacataacat
     1441 taggataaca ataataagag atttcatcaa aaaagagttc catttggtcg tcttttcccg
     1501 ttcgctgatc cttgttttcg tgattggttt attctttttg ctttctttac gataaaagct
     1561 ggcaatcttt ttttcttctt gctgataagc tgtttctgct tttcgctgtt tttttaatga
     1621 ctcgcttgct tgtgcttgtt gtcgcttgcg aagttcgctt cgcgtaataa gcggtccttt
     1681 ggccataagc cgatccctct actttccttt ggtcaatagt tcccagtatt gaatcgcata
     1741 aatcgtcttt gcatcacaaa tcaactgatc tttcatcgct tgttgcgcct cttcaagagt
     1801 tagatgatac aactccagta cttcgtcttc atcttgtgct aaaggattct cgaccttctc
     1861 taccccttgt gcatgataaa tatgaaggac ttcatttgca aaacctggtg aaagatacat
     1921 cgaagtaaga tggctcagcg acttcgcacg atagccagtt tcttcttcta attctctagc
     1981 tgctgtcatt tcgggatttt gtccttctcc tggatcgatt tttccagctg gaatctccaa
     2041 aatcactttt tctaaaggtt ttcgaaattg tttgacaaga agcagacgat cttgttcgtc
     2101 aaaagcaatg atcccgacac caccaggatg aaataccagt tctctcttcg aagttcctcc
     2161 atctggcaaa cgaacttcgt ctacagcaac atcaataatt tttccgctat agatttcttt
     2221 gcgactgatt gttttttctt caaattgctc attgttcagc atatcttctc ctgctttctt
     2281 tgtaatcata actgcttttt tttcattttt caatttttct caaaaactcc ttattagttt
     2341 accatagata aaccttagat tttctctaaa tatagaaaat ccttctttag tccgatactc
     2401 tttctagtag tccgtatgct aaaataagta tgtaatcgaa taacgaaaag aattcaatac
     2461 tcaacgataa atgacatgga gggtttatta tgataaaacg aaattggccg tggcttctag
     2521 cattggtctt gctatttctc tttgtccgaa acaatagttt ttcttttgtt ctaggacttt
     2581 tattgatagg tgtagtttta gttttgtggg gactctttgg ttcgcgcagg aaaaagggaa
     2641 aacaagaaga aattcctgca ctgactgacg aacttgaaac tcactatgaa aaaagcggta
     2701 tgacagcgaa tgaaatttca ttttttcgac aaacaatgaa tcaaacaaaa aatgagatca
     2761 atcaattgca aatgaatatg cagcagacag ccaaacttaa agcaatcgat ctccgccatg
     2821 atccagtcaa agcagcgaaa gctttattca aagaattggt gaaagaacca aaccgactgc
     2881 atgaagcaag tcagtttctt tatacgcatc tgccgaatct agttgatttg acaaataagt
     2941 ataatgagat caatgaccat gagatcaaaa acaaacagac ctatgaaaaa ctagaagaaa
     3001 gcgcgcagat catcgaccag ctgtcagctt taatcgcaca agattatcaa aaattcgttg
     3061 ctgaggatct agatgatatc gacgtagaga tatctgttgc gaaacagagc ttaaagcgtg
     3121 ataataaata tgaaccagaa caaaaggatt aactcttttg ttcataaaag aagactcgga
     3181 acattcagat gatgtctggc tgtttcgagt actttttcta aagtagagac attcatgtaa
     3241 gaatcgagaa cagattcaac aaggaggaat tttttatgga tcataaaccg actgaaacaa
     3301 aagatgttac accagttaac gatacacttg aagatttatt gaacaacccg ttttcaactc
     3361 caacagatac gctgaccact tcacaacaag cggagattga tcagctccaa gagcaacaga
     3421 cagctgctcg tttaattgac aaactaccgg cggaacgcca agaacaagct aaacagctag
     3481 cagcaaaaat cgatgctaat gatgcacagt ctgtcatcag ctacggatcg gctgcccaag
     3541 caaaattaag tgaattctca caatcgatgt tgaatcatgt gcaagcacaa gatattggtc
     3601 ctgtaggaga ttccttaact gaactgatgt atcgtctcca agaagccaat cctgacgaac
     3661 tgcgtgccgg agaagggaac atcttccagc gtgtcttcgg gaaggtcaaa caatcgatct
     3721 atgaagtgac agcaaaatat caaaagattg gtgcacaaat tgacaagatt tcagtgaaat
     3781 tagataaaga aaaagacggt ctgctaaaag ataatctgat gttagaacag ctttaccaga
     3841 aaaacaaaga ctactttgat gcactgaata tttatatcgc tgctggagaa ttgaaaatgg
     3901 aagaacttca aacaacgatc attccagaag cgatgaaaag agcagaagaa actggcgatc
     3961 agatggatgt tcagattgcc aatgattaca cgcaattcct agatcgtctg gataaacgta
     4021 cgcatgatct tcgcttagct agacagatca cgatccagca agcaccacaa atccgtttga
     4081 tccaaaacac aaatcaggcc ttggctgaaa agatccaagc atctatcgct acagccattc
     4141 cgctgtggaa aaaccaagtc gtgatcgcat tgaccttgct tcgtcaaaaa gatgcagtaa
     4201 ctgctcaacg ccaagtctct gaaacaacaa acgacttatt gaagaaaaat tcagaaatgc
     4261 tgaaaatctc agccatcgaa acagcgaaag aaaatgaacg tggaatcgtg gatatcgaga
     4321 cccttcaaac cactcaaaat gatttgatcg aaacaatcca agagacattg cgtatccaaa
     4381 aagaaggaaa agaaaaacgt cgtcatgcag aagtagagct tggacacatg gaagaagatc
     4441 tgaaacaaaa actgttggat ttaacccaat agatcacctt tcgcttgtta ctaaaacaaa
     4501 cgcccaacaa cagtttatta caactgttga tgggcgtttt tcttatcttt cttctaactt
     4561 ctccattgga gtctatttct cattcatctt gctgtgtcca tagccatagc caaaataaat
     4621 gatcagtcca ataaccatta cgataccgaa cacgatccat gtcacagttt gcagacgaag
     4681 catcaataca atacaagtca agatggagac gattggcaat agcggaacga atggcacttt
     4741 gaattctcca gcttttggtt tgccaaacat ttttctcaac ttcagtaagc ctagactaac
     4801 aagcatcaaa taggcgagcg ccatgatatt cgttagttcc gctaatacat tcaatgggaa
     4861 aaatccggca aagaatacag ctgccacacc agcggcataa gtcgcttttt tcggtgtacg
     4921 atgcttttga tcgatttctg tcataaactt aggaagtaag ccatccttgc tgacagcaaa
     4981 cagtaaacga gcaagtgaat acatcatcga gatcgttacc gtcagtagcg ttaaaattgc
     5041 ccctacagcg atcaccgcgc ccactgttcc atgtcctaca tacctcattg caaaagccac
     5101 tgggtcattg actcctagtt cagtatacgg aacgattcct gtcaagatca gcgtcacgat
     5161 gatgtaaagg acagtagcaa tcagtatcga accaatgatt cctcttggca catctttttg
     5221 cgggtttttg acttcctcgg ctgccattgc aacggcatca aatcccaaga aagcaaaaaa
     5281 cacaagagct gcaccatcta gcacaccgga aaaaccaaaa ggcatgaatg gctgccagtt
     5341 atcaggttta acgaaaaata ttcctacaat gataaacaac gcgatgatac caaatttgac
     5401 atataccatg gcattattca aacgtaatgc tttcttggct tcaagcgaca cgatgtaagt
     5461 gacgaatatc actacgagca ccgcaatcaa gtcaatgtat gtaccatttt ccggattgta
     5521 cccgccagtt aatgcttgtg gaagttcggc ccctaaactt ttcaaaaaac cttgcatata
     5581 gccagaccat ccggaagcaa cagaggaaac cgctagtaaa aattccccga tcaacaacca
     5641 ccctgtcagc caggcagcaa attcaccaaa tacaacatac agataggcat atggcccacc
     5701 aatcactggc actcgcgaag caaattctgc gaaacttagt ccagacaaaa tgacaacgat
     5761 tgcagctaag acaaatgaaa gtgacagtgc tggaccagca ttttgattgg ccgcaacccc
     5821 tgtcaccaca aatattcccg taccgatgat cgcaccaatt ccaagcatga tcaaatcaga
     5881 cgtttttagt tcttttttca tctcactcgt atgattgaga ttgatctctt tcttacgaaa
     5941 caaatccatt gaagttcctc cccattttag gttggaaaac agcgattaaa ctgttttcat
     6001 ataccctaaa aggataccac ctaaaatgac taaaaggcaa ccaacaataa cataaatgag
     6061 ctcttttttc gtttttcttt cgccaaggat gaaaatccca cctaaagtag agataatgat
     6121 ccccatttgt gaaagtgaga agccaacagc taaaccaagt gatttgactg ttagaagcat
     6181 gcagatgttt cctaagcccc ataataatcc acagatcatg tttttccaca caaactgatc
     6241 gactttaact ttacggaaag caaagaagct tgcgcctaag atcattccga tactttgagg
     6301 taagataatt gacatcgcat ccaaattcgc ccagttgacg atgatggtat atacaccata
     6361 accaatcgta gagaagatca atgctctaaa acccttacca aaatcaagca ttttattatc
     6421 tgtatctatt tttccttcgt catcttgtcg ggcagtcaaa tacgcaccgc ttacaagaag
     6481 agctaaagca ataataccgt atacataatc tttcgtttgt gtccattcat ggaagaaaac
     6541 ggctcccgca atcgtattca aaattaattg aaaaccagta gacaatggta gccctacaga
     6601 aacacccatg tacttcattc catggaactg tccattttgt ccaacactcc aagctaagcc
     6661 ggataaaaat ccgatcaaga aaatccaagg ggtgattaca ggactaacaa cgaaaaagac
     6721 aactaaagaa aacagaaatg caccgatcgt cattcctagt gtttgttgat tggcatcgcc
     6781 accaatttta ccacttacta aaccaatact accccatgcc aacataggga ctaatgctat
     6841 taaaatttcc ataaccaatc tccttttatt tcacatgata agaaacttat cagcgaaaac
     6901 gttaacaaca caagcacttt ttttcgtttc taacgatttt ctaacgtttt tcattataaa
     6961 aatttaagat tgggaaaaat aaagtcattg ttgtcccttt tccttcagtt gattcagccg
     7021 tcaaatggat acctaatttt tcggccagat tttttgctaa ataaagtcct aatcctgttg
     7081 aatgtgtttt gctaagtcga ccatttctcc cagtaaatcc cttatcaaag atccgtttca
     7141 agtcttccgc tgggataccg atccctgaat ctttaactga aaggctgact ccttgcgctt
     7201 ccttttctat gctcacaatg atgtttccgt gatccggcgt atatttgatg gcattactaa
     7261 gcaattggtt gaagataaaa gcgatccatt tagcatcggt caaaacagta tgatcctcac
     7321 caacgatctc gtagcgaata tttttttgga taaaataatt tgcctgactg cgtatcacag
     7381 gctgaatgat tccttttagt ccatattctt ggatgaggta gtcccgcgag aaactgtcga
     7441 gtcttgcata atacaaaact tgttctacgt attcatcgat ttttgataat tcattttcta
     7501 gtaatgtata cttttgatcg tcaatatcga actcgatcga acgaactagc aatttagaag
     7561 cggaaagtgg cactttgatt tcatggaccc atgagtcaat ataatctttt tggtcttgtt
     7621 gattactgat tgctgattgc attacttctt ggtgttcacg gatcagctga ttgatatatt
     7681 cttctaccag tttttcttct tctgtcctcc caccactcaa ataattttgc aaaggggaag
     7741 ttttttgtat gtcggctaat tttccccacc atcttctctt gctcaagtaa ccacccagca
     7801 gataaaaaat cagaaagacg ccctcaatca aagctaaata agcaattgtg ctcaaatcaa
     7861 ctttgatgtt tggcgccagc cacatcacaa agatcgtcaa tgcaataaaa aacagccatc
     7921 ccaagatcaa tgtccactga tctttcaaat atttaaaaaa attcatctta accgctcatc
     7981 ctttcaggga acgatatatc cctgtcctac ttttgtttcg atatatcctt ggatacctgc
     8041 ctgttcgatt tttcggcgta accgattgat gttgactgtc aatgtattgt catctacaaa
     8101 tcgttcgtca tcccacagtg ctcgcagcaa tttttcccga cttaagatct ttccattgtg
     8161 cttcatcaaa ataaataata gacgatattc atttttgctc aaatcaatga tctctccgtt
     8221 gatttccata cttccgttgt caacattcaa cgtaatgcca ttgtggctca tcatttcact
     8281 agataattct gtgtagttgt aagaacggcg aagtaatgca ttgatttttg caatcagtac
     8341 atcgatttgg aaatgttttg tcacaaaatc atctgccccc atgttcatag ccatgatcat
     8401 atccatattc gtattcctgc tggagataaa aatgatcggt actttcgata cttcacgtat
     8461 tttttgacac caataatagc catccaaaac gggaagatta atatctagta atacaagctg
     8521 tggttcttct ctttcaaagt ctgagaacac ttgattgaaa tcggtcgttc caaaggtttc
     8581 aaattgccat ttctcaagtt cctccatgat caattgtcga atgatctctt cgtcttcgac
     8641 taccatgatt tttgccataa aaaatccctc catctttctt cttgctacta caatatcaaa
     8701 gaagcccttg tgaaacaagt tggcttacct ggaaaatgtc attttagcaa acgttaaaaa
     8761 aaactggaaa tatgcgattt aatcgcgttt gtctcttcca agcagcttaa atttattatg
     8821 ttattcttaa taattggtct attatctgtt atttttcttt cgttgttttt gtcttttaaa
     8881 ttttcgataa caccaataac cgaaaagcgt tagcactagc aatataccta gaaggagtat
     8941 aagtaatcga taaagatggt atttttttgt ctgttctatc tttgaagaga cttcttctgt
     9001 aaagggaact cgtcttcctg tcactagtaa tcggtgagtg ttcacggtgt aaggtgtaca
     9061 agtgataagt gtgactagat cctgcttttc tctaattgct aatgtatttg tttttgttgg
     9121 aaggacaaca attttctcaa cgacttcata agcgagcatc cgaccataca cttcaagata
     9181 gaatcggtca tcttgctcca tttcttcaag atccgtaaag agttttttct cagcaatacc
     9241 gctatgggcg gtaatcacgg aatgtgtact ctctcctcca gttggaaaag aggtgccttg
     9301 caggactgtc gctcctctat ctaacaaaag atcattcgtt tcatcaaata aaggcaagct
     9361 aacattgatt ttcggaatat agatggctcc gatcatatgt tgttcgtaat attcttttcc
     9421 tggattttgg acatttctaa ccgcttgctc aaaaggatct ttgacttgtc ccattcctgg
     9481 gattactgat gtatgagcaa gcgtctgatt tttcttttca tgcactgcta attgttgctc
     9541 atgtttttct tctttatcgc gttcaagctg cttttgataa ttctcgatcc ttctttgatc
     9601 caaataattg ctgagagcat ctgctaaaaa tggatatgta aaaactgcag ctccggcaag
     9661 aaacatcact gccataaata attgtaataa aagactgatt tgggtttgag gtctattacc
     9721 tattttcttt ttcattttct tcaccttctt atttaaaaaa actgcagcaa cctttataaa
     9781 accgattgct gcagcttatt tttgttgctt atacttcaga cttcatttta gatttacggt
     9841 accagctgta cgctccaatc atcaaactta ttccgattag taagaatgcc aagagcccgt
     9901 tcccaccagt agcaggaagt gacccgcgtt tcatattcgg tactctttct ggccctggat
     9961 tttctactcc tacgatctga atttcttcat tatagcttcc ataagagatg ataaattcag
    10021 taaaggctgc ctcacctgtt ggaagtacat aaccttctgg cgccatggtc tctttcattt
    10081 gataggttcc ataatctaac ccttgaacct caaagtatcc atttccatct gcgacaaatt
    10141 ctgttgcttg tccgcttgtt gcccactcaa ttctatctgc tcctgattca taaacagcta
    10201 aaggcgactc ccctttataa aatattgcgt attcagaacg gtcttcattc cacaattcga
    10261 atcttgcatc tttaagcaat tcactgcttg atgcatcata tttttcaaac tttcttccac
    10321 ctgttgtgac tttctctggc ggctctactc cttcttcata atcaaattca tcatttcgtc
    10381 cgatttcaat aaccgctgtg ttgttatgga aatcatccgc ttctagttct gtggaaacct
    10441 tcaaattata cgtgatcgtt aaatctcctc cacgtgcatc tgctaatcta cctaaagtat
    10501 ctactttggc tactccattt tcttctgtca aaatcgtcac gacaaacccg ttgcttacta
    10561 cttctactat ataatcttgt ccttttgtta aaatcgtctc gccggctcgc acttctattt
    10621 cctgatcata ataagttaga ccaactgtag gcgtatctgt gattttgtaa tttttgataa
    10681 cagcgcttcc gttttcaagg acagaatcga tgaaataagg aataggtgct gtgatcgtgt
    10741 aagaagccgt ttttcctctt tctagatcaa tataagaaat cgtttcttgt tctccagctt
    10801 cattaataat cgttacttgt ctaaggtcgc tttcctctac attcatctgt ttatctgctt
    10861 ctctgatttc gtttttcgga tacaaatgca catctgtatt gtatgtgtct ttccatgtag
    10921 aaccatccca cacgccatcc accatatcag gcatcatcac tggcatcata acaaccattg
    10981 gagacgcgat tctggaaata tgagctggcg aataagtttc taagaaaaga tagactttat
    11041 ctctagaaga aggtgcttct tccgtcactt ggacggtaga gaataccgct tctccagctt
    11101 cattcgttgt ttgtttgtca atacttgtca gtcgatattt taaaaaccaa ctttctgtgt
    11161 gccgtttttg aatccattca atcgcttcgg cagaattaag accgtcttct cgttccggtg
    11221 ttaaaggatt atctcgaatc aaatcgtaat atacttccgt tacgtcaaac aaatcaaaat
    11281 cgactcctgg aatattttct tcaccaaaat ccgtagaaac caatccgcta ttcggtcgtt
    11341 cttccggaac agatgagaaa ccttttttgt gtaatgtaat cgtcacttca cttggttttt
    11401 gtgcgacttc ttcggctttg actagttttc cgttcccgaa aatccctatt cccgcagtaa
    11461 cgagtaacag tacactcatt cccgtgacaa tcattttttt attcattttt ttctccccgt
    11521 tcttttttta tt
//
LOCUS       DARBEC010000089        11224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.093, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000089 DARBEC010000000
VERSION     DARBEC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.093"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..1091)
                     /locus_tag="SCL80_002291"
     CDS             complement(243..1091)
                     /locus_tag="SCL80_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1108137.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            1090..2157
                     /gene="gnd"
                     /locus_tag="SCL80_002292"
     CDS             1090..2157
                     /gene="gnd"
                     /locus_tag="SCL80_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEG1108138.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            2159..3160
                     /locus_tag="SCL80_002293"
     CDS             2159..3160
                     /locus_tag="SCL80_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEG1108139.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALVERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            3157..3441
                     /locus_tag="SCL80_002294"
     CDS             3157..3441
                     /locus_tag="SCL80_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEG1108140.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            3474..4271
                     /locus_tag="SCL80_002295"
     CDS             3474..4271
                     /locus_tag="SCL80_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1108141.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNVAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            4285..5118
                     /locus_tag="SCL80_002296"
     CDS             4285..5118
                     /locus_tag="SCL80_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1108142.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIMGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            5136..5993
                     /locus_tag="SCL80_002297"
     CDS             5136..5993
                     /locus_tag="SCL80_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1108143.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLKQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            6015..6524
                     /locus_tag="SCL80_002298"
     CDS             6015..6524
                     /locus_tag="SCL80_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1108144.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            6561..6962
                     /locus_tag="SCL80_002299"
     CDS             6561..6962
                     /locus_tag="SCL80_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108145.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            7074..7427
                     /locus_tag="SCL80_002300"
     CDS             7074..7427
                     /locus_tag="SCL80_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108146.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            7461..7904
                     /locus_tag="SCL80_002301"
     CDS             7461..7904
                     /locus_tag="SCL80_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEG1108147.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            7921..9006
                     /gene="uxuA"
                     /locus_tag="SCL80_002302"
     CDS             7921..9006
                     /gene="uxuA"
                     /locus_tag="SCL80_002302"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1108148.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            8999..9712
                     /locus_tag="SCL80_002303"
     CDS             8999..9712
                     /locus_tag="SCL80_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1108149.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(9919..10068)
                     /locus_tag="SCL80_002304"
     CDS             complement(9919..10068)
                     /locus_tag="SCL80_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108150.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            10464..10664
                     /locus_tag="SCL80_002305"
     CDS             10464..10664
                     /locus_tag="SCL80_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1108151.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
BASE COUNT         3767 a         1752 c         2415 g         3290 t
ORIGIN      
        1 acaaaaataa taaaaaaaag aaaagataaa ttttttacta ctgtatacta actttgttat
       61 cgcatctaac aagtaactcg aataatacca atacaacctt ctccaaagat gcgatgacat
      121 acgttctaac ccaccgacta ttgatgttct gtaccaccct tgcagaacca aaaaagccac
      181 taccaaatat ttggtagtgg ctttttttgt tttatggatt tttcgctttt tctgttatgg
      241 tgctattctt tgatttcaga aatagtatgt cgaactttag cgatagaacg acgtgattct
      301 ttttcgttgt aaaacatcaa aatcacatat agagagtcca aaatgcttag ttgagaaata
      361 cgggaagcta aagcttcaga tcggaattct gtttcttcag aaatagaaat aaaaacgaca
      421 tcgccaagtt tagcaaggga agaattagct tggctagtaa taacgattgt ttttgctcca
      481 gtttttctta cggtttcagc aatatggatt gtttcttttg attgccctgt gtgagaaatc
      541 aaaacagcac agtctttttc cgtcaataaa gaagcttcca tcaattgaat atggtaatcc
      601 gtgctgtgag aagttgcgat aggggaacgc aaaaatttat gataagcgtc agttgctaca
      661 atctctgaac cgccaacacc gaaaaaatag acacgtttag attcattgat aatagtagcc
      721 gcttttttta gatccttttg ttgtaacaac ttttttgtgt catttaaagt tgaaatacta
      781 gactcgaaca ccttttgcgc catagtgagc tcagtatctg ttttctgaat attttcgtgg
      841 atagaaatag cagatagatc attttcttgg atcaacatgg ccattttaaa atctttaaat
      901 ccattataac caagtttttt tgtaaattga aaaaaagtag agtcagcaat gcccagttca
      961 cttgctaaat tgctaatagg actatgactt actttgcttg gattttctaa aatataatcg
     1021 gcgatagcct gttcttttgg gctgaagtcg ttgtacattg ttttgatttg tagtcttatg
     1081 gagacgctca tgagaattcc tcattttcat aataagatta tcattttttc cgttctcatt
     1141 aatgttaaag atacaaggag agtaggaaaa aatcaaacaa aaaatatttt tgtaaaatct
     1201 atttacaaaa aaattttttt gtataatgag ggtataagaa atggaggcgc tatcatggaa
     1261 attggtgtaa ttggtttggg aaaaatggga ttgaatttag ctttaaactt aaaagagcac
     1321 catcacaaag tacaaggact cgacatttca agtacagctg ttagtcaggc aagagaaaat
     1381 gacattcctg cttatcaaga gatctctgaa tttctagcta cttttgagca gaaaaaaatc
     1441 atctggttaa tgcttcctgc tggagagata accgaaaaca tgttaagaaa actttttcca
     1501 aaactcggac caggagacat tattattgaa ggcggaaact cttattataa agattcaatc
     1561 cgtcgagcag aagagtttca gaaaaatgaa atcggttact tggattgtgg gacttctgga
     1621 ggaatcgaag gagcacgaca tcaagcctgc ttaatgattg gaggagataa agaaactttt
     1681 ctatccgtgg aacaattatt caaagatgta gcaattgaaa gaggatatct gtatgctggt
     1741 gcatccggaa gcggacactt cttgaaaatg atccataatg gaatcgaata tggaatgatg
     1801 caagcaatcg gtgaaggatt ccaacttgta caaaaaagtg agtatgcttt tgacttggaa
     1861 caagtcgcac gtgtttggaa tcatggttcg gtgattcgcg gctggctgat ggaaatcatg
     1921 gaagatcagt taaacgaaca tcccaattta agtaattatc ggggaatcgt ttctgcatca
     1981 ggtgaagcaa aatggacggt tgaaacagcg ctagaaatgg ctgttccagt tccagtaatt
     2041 gcaactagtt tgttcatgag gaatctttca caggaagaag atagtttttc agcaaaagta
     2101 gtttcagcac taagaaatgg gtttggcgga catgaaatag ttgaaaaggg tgagtaaaat
     2161 gccagattat catatagcaa tcgttaattc cagcagtttc ggaagagtct ttccacacca
     2221 cttggaaaga ttaaagtcag ttggagacgt tcatttcttt actgttgatc aagagattcc
     2281 aggaaaagaa ctagcggagt tacttaaagg attcaatatc attattgcaa gtgttacgcc
     2341 attttttcga cgagattttt ttgaaaacaa agatgaactg ctattgattt cccgacatgg
     2401 tattggttac aacaatattg atttaaaagc agcaaaagaa caccagacaa tcgtatctat
     2461 catacctgct ttagttgaac gaaatgctgt agcagaaaat aatataacta atttgttgtc
     2521 catgcttcga cgcacgacac aagcacaagc aagagtcaag gacgatcgat gggaagatcg
     2581 ggcggagttc gtcggtagga cgctgtttaa caaaacagtt ggtgtgattg gtgcaggaaa
     2641 taccggaagc ggagtaatcg aaattttgcg taacggtttt cgctgtgatg ttctagctta
     2701 tgatccttac aaatcagatt tatacatcga aagctttggt gcaaaaaaag tagaacttca
     2761 agaattactg gaacaatcag atatcatttg tttatgtgcg aatttgacag aagagaacta
     2821 tcacatgatt tctgataaag aagtcgatca gatgaaagaa aatgtttatc tatctaatag
     2881 tgcaagaggc gctctagttg atgaagaagc gatgattaat ggtctgaaaa gcggaaagat
     2941 cgcaggatat gccacagatg ttttagaaga agaaccggga cgtcaagatc atccgtattt
     3001 agcgtttgaa caagtagtga ttacgccaca tatatcggcc tacacaaggg aatgtttaga
     3061 agcaatgggt gaaaaatgtg tcagagatgt cgaacagatc agtcagcacc ttcttcctga
     3121 acgttctgtc cagacaaaga gcaactatat aaaaggatga tgaagaaaat ggtgaacggt
     3181 ttaaaagtga aaacaggccc tcaattttat ttatatgaag aaggtggtat ttcaaaagtt
     3241 tctgatctct tgaaatcata cggcgcgaaa agagtactgg tcacccacgg aacggtatca
     3301 tgggaaaagg cgctacctaa attagtattt ttgaatgacg aaacgattca atttttttat
     3361 catcgttatt ctggagaatg tagttatgct gaagccagac gaatagctac aatcatcaaa
     3421 aaaatgaaat cgatttcgta gttggcgttg gcggaggaaa actgaccgat ctagtgggtt
     3481 atagtgccaa tcttgcaaga atcgatttcg gagtcatccc tactttagct agtaattgtg
     3541 ctccatggac acctcttgct gtcatgtata aggagaatgg acaagcagaa gggaaaacag
     3601 aacactttta cagacaagct gcttttttta taacagatcc ccaattagtg attgattccc
     3661 ctgttccata tttcattgcc gggttagcag atacactagc aaaatggtat gaatctgagc
     3721 tcatcttaga acaagatttt ttgcaagatg aatcattttt gaagctagca caagatactg
     3781 cgaagatctg caaagaagag attttgaatc attctgctaa agcaatccaa gatatggcac
     3841 aaagaaaact gacatcggag ttcaaacagc tttcagagat tatctttgct gttgctggtt
     3901 tagttggagg attaggagat aaatacgcaa gaaatgtggc agcacatgct atgcatgacg
     3961 ctatcagtaa atatattccg gaaagccatc gttttctaca tggagaaaaa gtagcgtatg
     4021 ggattttcta tcaactggca ctagaaggtc gctgggaaaa aatagacgag ttatatcctt
     4081 tgtatcggga attagaactg ccggtttctc tagaacaaat gggattattt ccgaaagata
     4141 aacagataat agataaaatg gctgaattta ttgattcgaa agagaaagtg catttgattc
     4201 ctatagaggt aacacagaaa tcactgatac agtcaatgat ggaactggaa ggttatatca
     4261 gaaagatctg aaggaggagg acacatggaa acattaagtg tagtacaaag tttgttgatt
     4321 gctttatggg tagccgcggt tatgtcccgc tggttaggtg gaggagccac gttgacctta
     4381 cgtttttctc ctttgatgac tggattagtc gtcgggttga tcatggggga agttgcacaa
     4441 gcgatgattg ttacagccgc tttgcaaatg atttatatgg gggttttttc tccaggagga
     4501 tcaatgccag ctgaaccatc tattgcagca gctatcgcag taccagtcgc attattaggg
     4561 gatctgtcgc cagaagcagc gattgccgtt gcggtgccag tcggattgct cggaagttac
     4621 ttgtatcagt ttcgtttttt catcaatact tttttaggta aatatacaga caaagctgta
     4681 gaagatttga atacaaaagc tatggcccga tcaattattc tttatccaac ggttgcctca
     4741 tttttattat tcgttccgct tgttttcatt gctttatatt taggggcgcc attgatcgct
     4801 gatgtaatca aagctttaga gggaacagtc gttatccaca ttcttgaagt agtaggtgga
     4861 gggttagcgg caatcggtat cgcgacaact gtatacgtta tcggtcgaaa agattatctg
     4921 gtctttttct tccttgctta ttttatgagt gttgttttga aatctttaga tattacgatg
     4981 gttacttatg ccatttttgg gattattatc gctttgatct ttgtccagtc gcaaaaaggg
     5041 aaacaagtac agcctgccac aagcaataac aattcagcat cttttgatga cgaagatgac
     5101 tatgatgacg gattttaatt taggaggaat gggaaatggc tgaaataatg gataatcaaa
     5161 caggtgctcc tgaagaaatc acgaaaaaag atgtaacaaa agcctatctg agatggcatt
     5221 ttgcaaacga aattcctcac tcttttgaac gttacttggc gccatcacta ctatacggaa
     5281 tgatgccaat cttgaaaaaa ctttataaag atgaagactc tttaaaagca gcatataaac
     5341 ggcagttact gttcttcaac actcaattaa gctggggtgg cggtgtgatt actggcctaa
     5401 tgtcttctat ggaacagcaa agagcagaag aagagtatga agaaaaagag atcttgatgc
     5461 aggatgactt gatgtataac acgaaagcgg gattgatggg ggcactagca ggaatcggag
     5521 atgcaatcga ttcaggaacc gtccaatata tttttatcgc aatcgcggta ccttgggcac
     5581 aacagggaag tcctttaggc gcactttttc cttttatctg ctttgggttg tatcaggttt
     5641 tactaggggt atttttcgca agacaagctt tcactatggg ccgtaatgct acaggcctaa
     5701 agcaaaacac tggtgttcag acagcgattg aaatgctatc agttttaggg ttgtttatga
     5761 tgggggtgct tgcgggaaac tatgtaaaag tagaatcttc attgaaattt gcgatatccg
     5821 gacgtgagtt tgttatccag gaaattcttg atcaaatcgt gccagggata ttacctttag
     5881 ctgtcgtaat gggtgtgtac ttgttctata cgaaaaaagg attgaaagtc acacaagcat
     5941 tattatggct gactggaata ttaattgtgc tagcaggaat cggtatttta taaaaagaag
     6001 ggaacgtgaa taagatgggt gtagtcaatt tagcgcgtgt ggatgagcgt ttgatccatg
     6061 ggcaagtaat ggtaacgtta tctcaaaaaa gtggagtgaa ttccattttt gttgtagacg
     6121 aagttgtagc aaaagataaa tttatgagag atctttacaa aaatgcagga agccgtacag
     6181 gacaaaaaac aattgtcatt acaccagaga aagctaaatt ctattgggat gaatatcaat
     6241 ttaaagaata caattgtatt ttgatcgcaa aaacagtcca aacgatttac gacttagtaa
     6301 aacacggaat ccccatggaa gaattgaata ttggagggat tgctcaaaaa aatcctgata
     6361 cagatctttt agtgacgaaa tctgtttatt taaataaatc agatgctgaa aaattgaaag
     6421 aaatgaatga acaatatggc gtaaagaata tttattttca agccactcca tctgcaccta
     6481 aaacaggatt aaaagatgta ttgaacaagt ttgatttgtc ataaacaaat gcttagaaaa
     6541 aagataaaga gggcgaaaaa atgaagaaga acgagctgtt tcgtgattgg gaatttcgtt
     6601 atcgctatat ttaccgtaag cgccgtacaa aaaagagtaa acagcgtttt ctttcagcat
     6661 tagtatcaga tatttacagt atgagaacag atgtgacagt cattgcttac gatacgctag
     6721 cctaccgatc aaaaaatatt tatgttggag atatcgaaaa agcagagaaa gttatctgta
     6781 cttattacga tacacccgtt catgcattag gaagttattt catgtttgat tggaaagacc
     6841 aaaggaaaaa aacaatctac tctatattat tgtccttcat tttgttgttc agtttaggtt
     6901 ggtggggaat gatgatatat aacaaaaatc ctcatcacgt attcgatctg ctatccgtat
     6961 aaacaggtat cactgtacta gcatttggga gctacttttt cttattggga aaagcagctc
     7021 gcggatggcc tagcagacaa acttttattc gaaatacttc atccatatta acgatgctag
     7081 aaatgatccg tacgattgat gatccgagtg tagcgtatgc gtttgttgat gaaggctgtt
     7141 atggagaaaa gggcttagac agcgtgagat cgggtatgaa aaaagaggct attctttttt
     7201 atctagactc tgttggggca gatacaccgc tacagttttc gggaaattat ttttcaaata
     7261 aagaacagtg gctgaaacaa gttgataagt tgaaagaaaa aaacgtcaat tatattttta
     7321 gtgcaagaaa aaagcaagcg cagttcttct atttgaccaa aacagattta cgagggaaaa
     7381 catttaattg gcaaaatgcc aatcagatta ttgctctttt tcgataagta aaaagtgcaa
     7441 aacaaggagt gagaagacta atgttggaaa tcatgatcgt tacacatggt aagttgagcg
     7501 aaggactgaa agatgcggcg gaagtaattg tcgggacatc gagccatatt gaaacaatgt
     7561 gcttagagtc tggggaggac atccaaacat tcggagagaa ggtcaaagaa aaattaacta
     7621 tgatcaacac agcagaaccc atgctagtat ttgttgatct aattagcgca agtccgtaca
     7681 atcaagcgct cttggcagtc aatgaactga ctgaagaaaa gaaagcagcc acttatgtca
     7741 tagggggcgt gaatctgcca atgttgttag aagcaatcaa tcatcagatg cttcacacac
     7801 cgatagaaga agcggtggaa caaattgtca aacaaggaaa agataattta actgtttggc
     7861 atgcttcagc ggaagaaaaa aaattagatg aggacgattt ttagaaggaa ggctgaaaag
     7921 atgaagtggg gatttagatg gtatggtgaa aaaggggatt cgatcccatt aaaatatgtt
     7981 cgccaaatac cgggaataaa tgggataatt ggaactttgc tgactaaaat gcctggagat
     8041 gtttgggaga tttcagaaat tgaggagttg aaagcatctg ttgagagcca aggactggaa
     8101 ctgcttggaa ttgaaagtgt agcgatccac gatgcaatca aggcagggac agatgaacga
     8161 gattactata ttgataacta tatacgaaca atcaaaaact tgtctcgatg tggggttcga
     8221 ttgatttgtt atagcttcaa accaattttt ggatgggcga aaactgattt gtttaatgaa
     8281 aacaacgatg gaagctattc tcttttatac gatcagaaaa tcgtcgatca gatggagcca
     8341 ggagaaatgt atcaattgat acacagccag tcaaagggat tcaaattgcc gggttgggaa
     8401 gaagaacggt tgaaaaaatt cgctcagctg gaaaaactgt atgaaggaat gaccgaagaa
     8461 gaattatttg aaaatctgac ttatttttta gaaaaagtga ttccagtgtg cgaaaaagca
     8521 gatgtaaaaa tggctatcca tccagatgat ccgccatggg aactttttgg ttttcctaga
     8581 gtgacgaaaa atcttggcga cttgaagaaa attctaaatg cagtagattc tccatataat
     8641 ggactaacgc tgtgcacagg atctttaggg gcaaatccgg aaaacgattt agttgaaatc
     8701 atccatgagg taggttcgcg catccatttt gtccacttca gaaacgtgga gtttttagga
     8761 gaaagaaagt tcaaagaaac ggcacatccc tctacagaag gctcccttga tatgtatcat
     8821 attatgaaag cgttggtgca agaagggttc gacggtccta tacgccctga tcatggtaga
     8881 accgtgtggg gtgaagtggc tatgcctgga tatggcctgt atgatcgtgc aatgggactc
     8941 acttacatgc aaggtctata tgaggcgata agtaaggaaa aaccactggg agagcattat
     9001 gaataaaatc aagctcttca tctttgatat ggatggcctt ttattagaaa ctggacgttt
     9061 agcttatcgt gcttatgtaa aatcagcaca aaagtatgat tatgaaatgc gtaaggaagt
     9121 ctattattta ctaactggcc aaacagaaat ggcgataaga gaacaaatgg gcattttgta
     9181 cggagaagat gttccatata tcaaatggcg agaagcaatc aatcaatata aagaaaaaat
     9241 cgtgaaagaa gataagagag tatatacgaa aaaaggagca gaagaaattt tatcttttgc
     9301 aaaagaaaga ggaatccata caatcgtggc ttcgtctaat acacgggtaa aagtagaaat
     9361 gtatttaaaa atggaaaatc tatatgcctt gtttgatcaa atcatttcag gggatgatgt
     9421 aaaaaaagga aagccggaac cagagatctt tttgaaagct tgttcaaaaa tgaatattcc
     9481 tccaagtgaa gcacttgtat ttgaagactc gattgcaggg atcgaagcag caaggcgagc
     9541 gggcattttg tcttttctca tcaaagatca tttgataggg ttgcccgatc ataatgggaa
     9601 gcataagttg aaagtagatg taagtttata tgaatccaat aaagcagact atgaatttta
     9661 tgacctgcat caagcaaaac gatttttaga aagtaaaggg ttgtatctct aataaagagg
     9721 actattttct agtcctcttt atttattgtc ctgcacgact gagggaacag atgaaaaaga
     9781 agtaattttt tattttgaag tatctacctt atttgtagtg aacaatttag aacaaacaca
     9841 aaacgcatct actgttatct accagtagat gcgtttaaag agaagaacga gaatcgtcca
     9901 tatgagggtt tccactcctt atgcagattt ttttaattct tgcactgctt gttcgatcgt
     9961 aacacctgtc gctacatgat tggtattttc tttgtcaaaa acgacaaata gattcaaatc
    10021 tgtatccaag gttactcgat attgtatatc tttgttatag aatgtcatag ccattctcct
    10081 ttctttaaat acttgctgaa aggcaacgaa cgcgttgata acgaaattcc atttataagt
    10141 ataccaccaa ttgaaaggtt ttcatagtta agaaatttta aagtcaaaag ttgcagataa
    10201 aagcaaataa acaagaaaac aaaaagtgaa tcaatcgcgg ttattcaact ttttcttcga
    10261 ttcgaagaca atagaagaca atgaatttta tatttcattg cattatcctt gtgttattag
    10321 aaaaagcatg atatattaaa tgagtaatct attttgaaac gtaatttgag cgatatatct
    10381 acatagataa aactcctttt gcctatcaaa tatttattgc aacgaaaaaa cgtacattga
    10441 tacgtatcag gaggaattta tttatgaata acggtacagt aaaatggttc aacgcagaca
    10501 aaggttttgg ctttatcact ggtgaagatg gaaatgatgt atttgcacat ttttcagcta
    10561 tccaaggtga cggcttcaaa actttagaag aaggccaagc agtgtcttac gatgttgaag
    10621 atggtcaacg tggccctcaa gcaacaaaca ttgttaaagc ttaataaaaa ttgactttta
    10681 accgtctttt cgaaagaaaa gacggttttt ttgtacgaaa aaattcattg gtcgttgaat
    10741 gggctgaata gtagtgtata aaacaatttt gtggagcgtg aagcttttat ggtagaggaa
    10801 aatacgaata aagtgatgtt gataaaaaga ttcctatctt gaatatgaat cgagatgtct
    10861 tgaaaatgaa tgagtttgac caatcattat cctatcgaaa tttacagttt cgttcgattg
    10921 gtctgtcaat taatgaaaaa gtaaaaaaga atatgttcta ggaagagggc ggcacatcta
    10981 aatttttaag taacggacag ttcaaatatc aagcattgaa agaggctatc ctactgtttg
    11041 aaggaatacg aatatataaa aacgacaaag aggaaaaagt agttcattaa atcccttcaa
    11101 gtgatgaaag aatgagatct aataaaacct gatctttcat ctgttttttt aataacgtaa
    11161 taattttttt attttttgtg ctttcgccgt tattgtagag tgatttagca ttcttatttt
    11221 taaa
//
LOCUS       DARBEC010000090        11144 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.094, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000090 DARBEC010000000
VERSION     DARBEC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11144)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11144)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11144
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.094"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..502
                     /locus_tag="SCL80_002306"
     CDS             <1..502
                     /locus_tag="SCL80_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108152.1"
                     /translation="IYKIEQNLELELLNESHYEEIYNFITDHPESMYFHEDIYHQGNS
                     KELEVVLRYNLFSFTSEFYFLRHKERIVGMVSIIVNPFQNLSYSIVNYLICEPAYTTT
                     ILTTVLENINEVSILEVKKLVIHLLNSTEDESLIPYLKDSHFFKEAVLSKEYREHDVL
                     RYAYIY"
     gene            566..751
                     /locus_tag="SCL80_002307"
                     /pseudo
     CDS             566..751
                     /locus_tag="SCL80_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347581.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
     gene            1325..1588
                     /locus_tag="SCL80_002308"
     CDS             1325..1588
                     /locus_tag="SCL80_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108153.1"
                     /translation="MFIKYGADTVATMFTEEYQVDYYEVIDKCVHFVYEKVWNFMNFA
                     ISEEILRVQENRTGIFTRDRKLNLIAHYIRNRWSSIPNFNYKK"
     gene            1922..2293
                     /locus_tag="SCL80_002309"
     CDS             1922..2293
                     /locus_tag="SCL80_002309"
                     /inference="COORDINATES: protein motif:HMM:NF016724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEG1108154.1"
                     /translation="MWLSDEKIQWRSYQKRILTNFATYLNNNSVHLVAPPGAGKTFLG
                     IEMIRRLDCKTLILVPSLILKKQWLTTIEEYFLVNPADSCYLSGAIEHPSHITVETYQ
                     TIYNRLKEEPTYGDQLELLVF"
     gene            2473..2895
                     /locus_tag="SCL80_002310"
     CDS             2473..2895
                     /locus_tag="SCL80_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108155.1"
                     /translation="MVKEEVLCPHQDFLYLIPANQETTQHYFTFLEKQNELVEALMES
                     QETVDYLLSLEFVQHPLEHSKEIYENFELFTSALIYLNHHNYELIPEHWQLLGSSKQQ
                     GKKASVPSLGKKELITIYQQMFEDQPELPIFYQLKKAG"
     gene            3040..3285
                     /locus_tag="SCL80_002311"
                     /pseudo
     CDS             3040..3285
                     /locus_tag="SCL80_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347587.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            3394..4350
                     /locus_tag="SCL80_002312"
     CDS             3394..4350
                     /locus_tag="SCL80_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108156.1"
                     /translation="MLILANQASTFVQTQQLRGRGLRLGAQNKVTNIWHLAIGLPGVP
                     LSEQPDLKRMLRRLGNISGLTFESSPKIESGGDRFALPLEYTTTTMETYTQSMFYFSC
                     ERSRLKEQWSTALADGWHLSMPIVVRKVDQVKQKQETSLPIVVKNIEPLSFWSAIFKG
                     NLLEYFQIRRRQKLWEKECARKKSLAICVQTTLQRLGEVSDRKVIVAADQQEFRCYLE
                     NAGYREGQLFTEAMRELLAPLENPRYLIEGKKIVLTVPERFGRNKQIANLFLNELNQS
                     SLPKCQLVYTRNPEGRQKLVTYRMRQVFQTRDEVIIQKNIWT"
     gene            complement(5186..5857)
                     /locus_tag="SCL80_002313"
     CDS             complement(5186..5857)
                     /locus_tag="SCL80_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1108157.1"
                     /translation="MTIKTSLQNQAYQSIRQQIIYADLEPGKKVSEKKLEETLSIGRT
                     PIREALIQLRHQGLVDTIPQSGNYISLIDLDLAKNARFVREHLERQIMLECCAKMNNQ
                     KKQVLETIIAEQEKAVSQKNARSFFHNDNLFHKACFEIAEREEVWQWLEDNNTHLERF
                     RWLRVKTEGLKWEKIMEQHHQLFKALTMQDPEEANFLTSLHLHLMLDEQNVVLTTHPK
                     YFKNI"
     gene            6174..7805
                     /locus_tag="SCL80_002314"
     CDS             6174..7805
                     /locus_tag="SCL80_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1108158.1"
                     /translation="MVLIKDWQDSIEGLNKMQVQLPYFNVEAVKQEGQKHPVWLHFGG
                     GNLYRGFHAEIAQCLADQNELESGVVVCETFDEEVVEKAYHDYNNDILEIVMGEDGEL
                     KKRLLAVTADSLYCNPKNITDYQRVVKYFEDPTLQFTTFTITEKGYSLKDSKGQFFSV
                     VEADIKSGPTNPQHTMSIVTSLLYSRFNAGELPIAMVSTDNFSQNGQRFQDSIFMIAE
                     AWQKKGYVSEKFIAYLKDKEKVTFPWSMIDRITPNPSESVKMDLLKAGFEDLDIIHTA
                     KNTNIAAFTNTEKVHYLVIEDSFPNGRPKLEAAGVIMTDRETVDKADIMKVTTCLNPL
                     HTALAITGCLLGYKSIQAEMQDDDLVGLIKGIGYVEGLPVVESPGVIDPKQFIDEVIN
                     KRLPNPYIPDTPQRIATDTSQKVAIRYGETIKKYVEKDGNAENLTFIPIAIAAWLRYL
                     LAVDDQGKNFVPSPDPLLVELQEKFSVLKLGEQDPAKIHCAVQEILENKEIFGLDLYQ
                     VEIGKKIEKIFCEMLVGPQAVRKTINKYITEVGGK"
     gene            7808..8887
                     /gene="uxuA"
                     /locus_tag="SCL80_002315"
     CDS             7808..8887
                     /gene="uxuA"
                     /locus_tag="SCL80_002315"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1108159.1"
                     /translation="MEMTFRWYGANEEKISLQEIRQIPAMKGIVGTLMDIPAGEVWPR
                     ERIKALKDEVEANGLTLKVIESVNVHDDIKIGLPTRDQYIENYKQTIRNLAEFGIEVI
                     CYNFMPVFDWVKSDLDYHLDDGSSTLAYISKNIPTDPEEIIRKVENSTNNFELPGWEP
                     HRLSQIKQLLEAYKEVDEAKLRENFAYFLKAIIPTCEEVGIKMAVHPDDPPYSIFGLP
                     RIVKNRDDLEWLCNVVDSPSNGITLCTGSIAERPDNDVYEILSEFCQRDRIPFAHVRN
                     IKYLSDNGLDFYEAPHKSEYGSLDMYKILKTMYDNGFNGYIRPDHGRMIWGETGRPGY
                     GLYDRALGAVYLNGIWEALEKGASK"
     gene            8961..10388
                     /gene="uxaC"
                     /locus_tag="SCL80_002316"
     CDS             8961..10388
                     /gene="uxaC"
                     /locus_tag="SCL80_002316"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEG1108160.1"
                     /translation="MFLDKNFLLENEIAKRLFHKFAEHQPIIDYHCHLDPACLLENKQ
                     YKNLTQVWLNDKGAGDHYKWRLMRANGIEEELISGAGNDYEKFLAFVKTVEKAPGNPI
                     FEWSHLELQRYFDIDLTISEKNAPEIWRRANEKIQTDSYRPKELLKMMDVKAICTTDD
                     PTDDLRYHTELSKQDNSFKVLPTFRPDKVWSIKSSGFSNYILKLSQAAGIPIKNFNGL
                     KQALSQRVDYFHQIGCRLADHGLNTYFYEIADEKILDNLLIKALAGKTDFHLSEIAQF
                     VTGIQLHLMSEYTRYGWTMQMHLNALRNNSSILKEQIGVDVGGDSMGDQASLTQNVVA
                     LFDTAEKSGCLPKTILYSLNPTDWLPLATAMQSFQGGMIQKIQLGCAWWFNDTAEGMR
                     QQLTTMAQQSLLSNFTGMLTDSRSFLSYPRHEYFRRVLCQLLSEWVKAGRIPDDEMYL
                     SQIVENICYRNAYNYFNFFEIESRK"
     gene            10472..11119
                     /locus_tag="SCL80_002317"
     CDS             10472..11119
                     /locus_tag="SCL80_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HEG1108161.1"
                     /translation="MKKVEILSKLEAAGVIAVVRGKNKDEAIKAINAITAGGIKGIEL
                     TFTVPNAEEIIKEIAKKYADQKDVIIGAGTVLDAFTARIAIMAGAEYIVSPSFDRQTA
                     EICNLYQIPYLPGCMTITEIKEALTAGVEIIKLFPGSAFGPSVVKTYQAPLPYVNIMP
                     TGGVSLDNLEEWFEAGVVAVGVGGNLLAPVEKGDFEEVTRIASKYIDKFYEIKNY"
BASE COUNT         3843 a         1576 c         2202 g         3523 t
ORIGIN      
        1 aatatataaa attgaacaaa atttagaatt agaattacta aatgaatcac attatgaaga
       61 aatttataat tttatcacag atcatcctga aagtatgtat tttcatgaag atatttatca
      121 tcaaggaaat agtaaagaat tagaagttgt ccttagatat aatttatttt cgtttacgag
      181 tgaattttac tttttaagac ataaagagag aattgtagga atggtttcta taattgttaa
      241 tccatttcaa aacttatcct acagtatagt aaattacctc atatgtgagc ctgcatatac
      301 tacgactatt ttgacaacag tattagaaaa tattaacgaa gtttcaattt tagaagtaaa
      361 gaagctagtg attcatttgc taaattcaac agaagatgaa tcactaatcc cctatttaaa
      421 agattcacat ttttttaaag aagcggtact atctaaagaa tatagagagc atgatgtttt
      481 gagatacgct tacatttatt aaataaaatt tctgatagta ttttaataat tcggatcata
      541 tcataagata taggagggaa aagaagtgat tttattgatt ttgtctctcg tattactagt
      601 tgtatctaat ctaatgaaaa ataatcctcc tacaaaaatt aattcttttt atggctttag
      661 aactaaaaag tcaatgaaaa atgaaaaaag ttggcattta gcacaaaaat tgttggggga
      721 tttttcaaaa aaaaaagtta tcccagtatt tttgatgggt attgtttttt tcagtataga
      781 gatttatagt ttagctattt taaaaaatga aaattttttc gttagtgtat taatgggtga
      841 aagtttaatc ctaatactga ttttgtgttt aattgttcat gaagtgaata aacaattgtg
      901 aattaaaaaa ataaaaacct agtataatca ctttaataaa ttgttggtag aaggtgatca
      961 aaatgggagt aaacgaaatg atgaaagtca gattgagttt ttaaatgata ccttcctcag
     1021 aacttcaaaa cgaattcaaa gattcgttga caaatcttga gcaagatcat ttaaaaaaat
     1081 attttctctt catttgacga aaaatgttta gaaatcttat ataagtgaag ataaagtaat
     1141 tggtgtaatt aattgtagag aatagccttt gagcaattga agcgatacaa ttatttagaa
     1201 aatgaacttc ctccagcata ctacatgatt ccttacggga cgatgttaaa gaagaaagca
     1261 agaaatttta gaagtattag tagataaact aatgggcttt tctcaattta agggagtagc
     1321 ccaaatgttt atcaaatatg gtgcagatac agtagcaacc atgtttacag aagaatatca
     1381 agtagactat tatgaagtaa tagataaatg tgtacatttt gtttatgaaa aagtgtggaa
     1441 ttttatgaat tttgcgataa gtgaagaaat cttacgagta caagaaaaca gaacaggaat
     1501 atttaccaga gacagaaagc ttaatttgat tgcgcattat atacggaatc gctggtcatc
     1561 aataccaaat tttaattata aaaaataatt atgaacaatc ttttatacaa tatacacaat
     1621 aaatcaagta gtatttatga aaaaagactt caaaaacaga atgattttct atcagtctta
     1681 gaaattatag agctaaaaat tttggtcacg cgtagaaacg cgtttttttt gtttttttga
     1741 tagtaaagaa cgtacaaaac aatgagaatt taaaagattg tactaccagt ttttttacct
     1801 tttcatttga gtttagagta agtaaaatat tactataaaa acacatattc ttgaagaaat
     1861 cttttttttg ctgtattcat ttagattatg atagagaaaa tacgtgagag ggggacctgg
     1921 gatgtggcta tctgatgaaa aaattcagtg gcgtagctat cagaagcgta tattgactaa
     1981 ctttgctact tatttaaata acaattcagt ccatctagtg gctccaccag gggctggcaa
     2041 gaccttttta ggaattgaga tgatccgacg tttggattgt aaaaccttaa ttttggttcc
     2101 ttccctaata ttgaagaaac agtggttaac aacaatcgag gaatatttct tagtaaatcc
     2161 agcagatagt tgctatcttt ctggggcgat tgaacatcct agtcatatta cagtggaaac
     2221 gtatcagacc atttacaacc gactgaagga ggagccaact tatggtgatc agctggagct
     2281 gttagttttt tgatgaagct catcatttaa aaaaaagctg gggtgaattg ttgattgact
     2341 tgaagaaaaa acaaccgaat ctgttgacga tttccttgac agccacgcct ccgattgata
     2401 gctctcagac ggagtggaag caatatattg agttaaaatg gcctgattga cgaagaaatt
     2461 agcattcccg agttggtaaa ggaggaggtt ctatgtcctc atcaggattt tctatatttg
     2521 attccggcta atcaggaaac tactcagcat tattttacct ttttagagaa acaaaatgag
     2581 ctggtggaag cgttaatgga aagccaagag acggtggatt atttgctttc tttagaattt
     2641 gttcagcatc cgcttgagca ttcgaaggaa atttatgaaa attttgagct gttcactagt
     2701 gcattaattt acttgaatca tcataactat gaattgatac ccgaacattg gcagctatta
     2761 ggcagttcta agcagcaagg taaaaaagct tcagttccat ctttaggtaa aaaagagctg
     2821 atcactattt atcagcagat gtttgaagat caaccggaat tgcctatttt ttatcaattg
     2881 aaaaaagctg gatgattgtt tgatgagtcg ctgcatttgt ttccagagta tccagacact
     2941 gttggtagtc aggcgattcc tgagaaaaag caagcagttt gtcaaatttt gttaaaagaa
     3001 gaagttcagc tgaagcaaga actgacagcg gtcattttga tggatcgtat ttgcaaggag
     3061 gtattagcag gcagtgatgg taccttagct tatggcgtgg ttcctttgtt tgatgaagtt
     3121 gttcgtctat taggtaacac tcagcaaatc ggtgccatct gtggagagtt cttactgtta
     3181 ccgaaaatgc tagtaactgt tgagctggca gaactagcga cgggtatgaa tgaacatcct
     3241 tatatgtcgg gttattttta tttgacggtg gatgacaaaa accgccaagc attgatgcaa
     3301 gcagttactc gcctattaac cgaaaagaag ttgaatattt tggtaggaac cgttagtttg
     3361 ctaggagaag gctgggattg tcctgcaatt aatctgttga tcttggctaa tcaagcttcc
     3421 acttttgtgc aaacacaaca actacgaggg cggggcttac gcctaggtgc acaaaataaa
     3481 gtaacgaaca tttggcattt agcgattgga ttaccgggag ttcctctgtc tgaacagcct
     3541 gatttgaaaa gaatgctacg gcgtttgggc aatatttcag gtctgacctt tgaatcttca
     3601 ccaaaaattg agagtggtgg cgatcgcttt gctttaccac tggaatatac gaccaccacg
     3661 atggaaactt atactcagtc gatgttctat ttttcttgtg aacgaagtcg gttgaaagaa
     3721 caatggagta ctgctttagc tgatggctgg cacctatcta tgccaatcgt cgttaggaaa
     3781 gtcgatcagg taaagcaaaa acaagaaact tcattgccga tagtagtaaa aaatatagag
     3841 cctctaagct tctggtcagc tatatttaaa ggtaacttgc tggaatattt ccaaattcgt
     3901 aggcgacaaa agctatggga aaaggaatgt gcccgcaaaa aaagcttggc catatgcgtt
     3961 caaacgacgt tgcagcgcct tggtgaagtt agtgatagaa aagtaatcgt agcggctgat
     4021 cagcaggaat ttagatgcta tcttgagaat gctggctatc gagaaggaca gctttttact
     4081 gaagctatga gggagctttt agcaccgctg gagaatccgc ggtatttaat cgaagggaaa
     4141 aagatagttc tgacggtacc agaacgcttt ggccgaaata agcaaatcgc taaccttttt
     4201 ctaaacgaat taaatcaatc gtcgctgccc aaatgtcagt tggtgtatac tagaaatcct
     4261 gaaggacgcc aaaagctagt gacttatcga atgaggcaag tcttccagac ccgcgatgaa
     4321 gtaattattc aaaaaaacat ttggacgtaa gttgatgaaa ataaattttg ctagagtaga
     4381 acaagtccga gaattaagtt gctacttaac tgataatgcc aaggacagga gaatcttttc
     4441 ttgacgactg aaagaaacta tttgacttat ataacggtgt gtgatgaata tttgcaaggc
     4501 attctcttaa aaagtactcg taatagtttt tttgaaaaca aaacagtact ttattaatag
     4561 gactgacaga gcgatataag aacatctgca gacagaggtg aatgcttttc tcatgatcga
     4621 tgaaacaatg gtacttcgga tattcttgct tttagtttga accgttgtga ttttctttga
     4681 ttcctaaaag tagttttata gaatgaaaat caagcatacg gaatgctcct ttgattgtgt
     4741 tgtggtgatt caatctaatg gagcttctat atgttttttt atttgcaaaa agaaccgata
     4801 gaatattttt atcagtctta gaaattatag agccaagaaa tttcaagcac acataaaaac
     4861 gcgtgtttta tgcattttat gagcaaaaat aactaaagac cattacgtaa aacataggta
     4921 aatcagcatt caatctcaaa ttatgtgatc aaatgttgat attatttgat tataagtttt
     4981 cttttgctat tatcagtctg caaataaaca gattatagag tatgaatgta gatagttcaa
     5041 tctttgttta cgacctcaca tgttttttta ctcaagagaa agtaatataa agtgactata
     5101 tttgacagaa ctttacataa aaaaagtagt tagttttctg tagtaatcag aaatgtaaat
     5161 aaattaaaaa aaagaaattt agcagttata tatttttaaa atattttggg tgagtagtca
     5221 atacaacatt ttgttcatca agcattaaat gaagatgcaa ggatgttaag aagttagctt
     5281 cttcagggtc ttgcatggtc aaggctttaa atagttgatg atgttgttcc ataatttttt
     5341 cccattttaa tccttccgtt tttactcgta accaacgaaa tcgttccaag tgcgtattat
     5401 tatcttccaa ccattgccat acttcttccc gttctgcaat ttcaaaacaa gctttatgaa
     5461 ataaattatc attatgaaaa aaactacgag cattcttttg actaacagct ttttcttgtt
     5521 ctgcaattat agtttctaaa acttgtttct tttgattatt catcttggca caacactcta
     5581 gcataatttg tcgttctaaa tgttctctga caaaacgagc attttttgct aaatctaagt
     5641 cgattaatga gatataattt ccactttgag gaattgtatc aacaagtcct tgatgacgga
     5701 gttgaatcaa tgcttcacgt attggagtac gaccgataga aagcgtttct tccaattttt
     5761 tttctgatac tttttttcca ggttctaagt cagcgtagat aatttgctga cgaatagatt
     5821 gataagcttg attttggaga ctagttttaa ttgtcatatt taatccctct caaaatttca
     5881 tttttgaatt tgatatttta gttctatgct cgttatataa tatcaaattt caaagtaaca
     5941 tactagctat taaaaaaaca aaaaatactc aaataagatc aaagagaaat taagctttaa
     6001 tttaagttag ttctaaaaaa tatatattgg tgtgctttta ctaaaaaaag acacaataat
     6061 tctgacatgg tagaaaaaag aatattttta gttttttctt attgacagcg attacattcg
     6121 attttatact acaattgtaa cggaaactaa catatcagta tggaggggta aatatggttt
     6181 tgattaaaga ctggcaagat tctatcgaag gattgaacaa aatgcaagtt caactaccgt
     6241 attttaatgt cgaagcggtc aaacaagaag gtcagaaaca tccagtgtgg cttcattttg
     6301 gtggaggaaa tctttatcga ggatttcatg cagaaattgc tcaatgctta gcagatcaaa
     6361 atgaattaga atccggagta gttgtgtgtg agacatttga tgaagaagta gtagaaaaag
     6421 cttatcatga ctacaacaat gatattttgg agattgttat gggtgaagat ggtgaactca
     6481 aaaaaagact tttagcagta acagctgata gtttatattg caatccaaag aatataacgg
     6541 actatcaaag agtggttaaa tattttgaag atcctacatt acagtttaca acgtttacta
     6601 ttactgaaaa aggatacagt ttaaaagatt ctaaaggaca atttttttcg gtagtggaag
     6661 cagatataaa atctggacct accaatcctc aacatacgat gagtattgtg acatccttgt
     6721 tgtatagcag gtttaatgct ggagagttac cgattgcgat ggtttctact gataatttct
     6781 cacagaatgg tcaacgtttt caagatagca tttttatgat tgcagaagct tggcaaaaaa
     6841 aaggatacgt ttctgaaaaa tttattgctt atttaaagga taaagaaaaa gtgacttttc
     6901 cttggagtat gattgataga attacgccaa atccttcgga aagtgtcaaa atggatttat
     6961 tgaaggcggg tttcgaagac ttagatataa ttcatactgc gaaaaatacc aatatcgctg
     7021 cttttaccaa tactgaaaaa gtccattatc tagtcattga agatagtttt ccaaatggtc
     7081 gacctaaact ggaagcggct ggtgtgatta tgacagatag agaaacagtt gataaagcag
     7141 atatcatgaa ggttacgaca tgtttaaatc cgctccatac ggcattagca ataacaggtt
     7201 gtttgttggg gtataaatca attcaggcag aaatgcaaga tgatgatttg gttggcttaa
     7261 ttaaagggat aggttatgta gaaggcttac cagtagttga aagtccaggc gttattgatc
     7321 caaaacaatt tattgatgaa gtaatcaata aacgtttgcc taatccctat attccagata
     7381 caccgcaacg aattgcaaca gatacttctc aaaaagtagc gattcgctat ggtgaaacaa
     7441 ttaaaaagta tgtcgaaaaa gacggaaatg cagaaaatct aacatttatt cctatcgcta
     7501 ttgctgcttg gttacgttat ttattggcag tcgatgatca aggaaaaaat tttgtgccaa
     7561 gtcctgatcc tttattggta gagttgcagg aaaaattctc agtattgaaa ttaggtgaac
     7621 aagatcctgc aaagattcat tgtgcggtac aggaaatctt agaaaataag gaaatatttg
     7681 gtttagattt atatcaagtt gagataggaa agaaaattga aaaaatcttt tgcgaaatgc
     7741 tagtaggtcc tcaagcagtt cgtaaaacaa tcaataaata tataacagaa gttggaggaa
     7801 agtaactatg gaaatgactt ttagatggta tggggcaaat gaagaaaaaa tctcactaca
     7861 agaaattcgt cagatcccag caatgaaagg aattgttgga acattaatgg atataccagc
     7921 cggagaggtc tggccgagag aacgaattaa agctttaaaa gatgaagtcg aagccaatgg
     7981 ccttaccttg aaagttattg agagtgttaa tgttcatgat gatatcaaaa ttggtttgcc
     8041 aacacgagat caatacatcg agaattataa acagacgatt cgcaatttgg cagaatttgg
     8101 gattgaggtt atctgttata attttatgcc agtctttgat tgggtaaaat cagatttgga
     8161 ttatcattta gatgatgggt cttctacgtt agcctatatt tcaaaaaata ttcctactga
     8221 tccagaggaa attattagaa aagtagaaaa ttctactaat aattttgaat tgccgggttg
     8281 ggaacctcat cggttatcac aaatcaagca attattagaa gcctataaag aagttgatga
     8341 agcgaaattg cgagagaact ttgcatattt tctcaaagca attatcccga catgtgaaga
     8401 agttgggatt aaaatggcag ttcatccaga tgatccacca tattctattt tcggattacc
     8461 tcgtattgtt aaaaatcgtg acgatttaga atggttgtgt aatgtagtgg attctccttc
     8521 taatggtatt acgttatgta ctggaagtat tgcagaacga ccagataatg atgtctatga
     8581 aattctatct gaattttgtc aacgggatcg gattcctttt gcacatgttc gtaatatcaa
     8641 atatttaagt gataatggac tcgattttta tgaagcgccg cataaatcag aatatggttc
     8701 tctagatatg tataagatat taaaaacaat gtacgataat ggatttaatg gctatattcg
     8761 tccagatcat ggtcggatga tttggggaga aaccggtaga cctggttatg ggttatatga
     8821 ccgagctcta ggtgcagttt atttaaatgg tatttgggaa gcgcttgaaa agggggcgag
     8881 taaataatta tataatggag tctatttaga agaaaaattt tctcttctaa aaactaaggg
     8941 aaggaaagga gtgcttaaat atgtttttag ataagaactt tttattagag aatgaaatag
     9001 ctaaaagact ttttcataaa tttgcagaac accaaccaat catcgattat cattgtcatt
     9061 tagacccagc atgtttgcta gaaaataagc aatacaagaa tttgactcag gtatggttaa
     9121 atgataaagg agctggagat cattataaat ggcgattgat gcgagctaat gggatagaag
     9181 aagagttgat cagtggtgca ggtaatgatt atgaaaaatt tttagctttt gtaaaaacag
     9241 tggagaaggc gccaggtaat cctatttttg aatggtctca tttggaatta caacgttact
     9301 ttgatattga tttgacaatt tctgagaaga atgcaccaga aatttggcga cgtgccaacg
     9361 aaaaaatcca aacggattcc tatcgcccaa aagaattgct gaagatgatg gatgtgaaag
     9421 ccatttgtac aacggatgat cctacagatg acttgcgata tcatactgag ctttctaaac
     9481 aggataattc tttcaaggta ttacctactt tccgtccaga taaggtctgg agtatcaaat
     9541 catcaggatt ttcgaactat attttaaaac ttagtcaagc agcgggtata ccaatcaaaa
     9601 actttaatgg gttgaagcag gcattgagcc aacgagtgga ttattttcat caaattggct
     9661 gtcgattagc agatcacggt ttgaatactt atttttatga aatagcagat gaaaaaatac
     9721 tcgacaatct gttaataaaa gctttagcag gaaaaacgga tttccattta tcagaaattg
     9781 cacaatttgt tacaggtatt caattacatt tgatgtctga gtatacaaga tatggttgga
     9841 cgatgcaaat gcatttaaat gctttaagaa ataattcaag tatattaaag gaacaaattg
     9901 gagtagatgt cggtggtgat tcgatggggg atcaagctag tttgacacaa aatgttgtag
     9961 cattgttcga cactgctgaa aagagtgggt gcttaccaaa aacaatttta tattcattaa
    10021 atccaaccga ttggcttcca ctagcaacag caatgcaaag cttccaaggg ggtatgattc
    10081 aaaaaatcca attgggatgt gcttggtggt tcaatgatac agctgaagga atgcgtcaac
    10141 agttgactac gatggcgcaa caaagtttgt tgtcgaattt tacaggtatg ttgactgatt
    10201 ctcgaagttt tctaagctat cctcgccatg agtatttccg tcgagtatta tgtcaattac
    10261 taagcgagtg ggttaaagca ggtcgaattc cagatgatga aatgtatctt agtcagatag
    10321 tggagaatat ttgttatcgt aatgcgtata attattttaa tttttttgaa atcgaaagta
    10381 gaaaataata caaaaaataa aagcttaaat ttatttattg taagttagta caactagcta
    10441 gagtatgtat ccataaaaga aggagataga tatgaaaaaa gtagaaattt taagtaagtt
    10501 agaagcggct ggagtcattg cggttgtacg tggaaaaaat aaagatgagg ccattaaagc
    10561 aataaatgca attactgcgg gtggaattaa agggattgaa ctaactttta cagttccgaa
    10621 tgctgaagaa ataatcaaag aaattgctaa aaaatatgct gatcaaaaag atgtaataat
    10681 tggagccggt acagttttag atgcgtttac tgctcgtatt gcaattatgg ctggagcaga
    10741 atatatcgta agtccatctt ttgatcgaca aacagcagag atttgtaatt tatatcaaat
    10801 tccttattta ccaggttgta tgacaattac tgagataaag gaggcactta ccgctggagt
    10861 agaaatcatt aaactatttc caggttctgc ttttggtcca tctgttgtaa aaacgtatca
    10921 agctccatta ccatatgtaa atataatgcc tactggcgga gtaagtctag ataacttaga
    10981 agagtggttt gaagcaggcg ttgttgcagt tggtgtaggt gggaatttgt tggctcctgt
    11041 tgaaaaaggt gattttgaag aagtaactcg aatagctagt aaatatatag ataagtttta
    11101 tgagataaag aattattagg agaaaaaata aataatacta taaa
//
LOCUS       DARBEC010000091        10930 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.095, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000091 DARBEC010000000
VERSION     DARBEC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10930
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.095"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..287)
                     /locus_tag="SCL80_002318"
     CDS             complement(<1..287)
                     /locus_tag="SCL80_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108162.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAE"
     gene            429..1547
                     /locus_tag="SCL80_002319"
     CDS             429..1547
                     /locus_tag="SCL80_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEG1108163.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            1675..3051
                     /locus_tag="SCL80_002320"
     CDS             1675..3051
                     /locus_tag="SCL80_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEG1108164.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            3452..5032
                     /locus_tag="SCL80_002321"
     CDS             3452..5032
                     /locus_tag="SCL80_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEG1108165.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            5230..5453
                     /locus_tag="SCL80_002322"
                     /pseudo
     CDS             5230..5453
                     /locus_tag="SCL80_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
     gene            complement(5658..5978)
                     /locus_tag="SCL80_002323"
     CDS             complement(5658..5978)
                     /locus_tag="SCL80_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEG1108166.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(6126..8360)
                     /locus_tag="SCL80_002324"
     CDS             complement(6126..8360)
                     /locus_tag="SCL80_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1108167.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            8839..9105
                     /locus_tag="SCL80_002325"
     CDS             8839..9105
                     /locus_tag="SCL80_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEG1108168.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            9105..10832
                     /gene="ptsP"
                     /locus_tag="SCL80_002326"
     CDS             9105..10832
                     /gene="ptsP"
                     /locus_tag="SCL80_002326"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEG1108169.1"
                     /translation="MVEMLKGIAASDGVAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
BASE COUNT         3480 a         1912 c         2215 g         3323 t
ORIGIN      
        1 ttttctgctt cttcaatcaa tatttttcct aacccttggt gtctgtattc agaaagaatc
       61 gccatccgct gtaatttcat ttttccgttt tctaaaggca aaagacgtac agttccttgc
      121 ggttcttttt tatctgagta caggacgaag tggatacaat gggcttcatc tttgtctatt
      181 tctcgagaca aaggcacacc ctgttcgact acaaaaactt gatgcctgat ccgtacagca
      241 tctaagtaaa tattgctcat tgtatctttt gtttgtacaa ctttcatggt ctagccacct
      301 tcctttcgta tagtatactg gaaaataaaa caaataaaaa gtatggcaag ttttcaaaaa
      361 taaaaaaaga ataaaagaat ttttaacgaa tataatatat actagtaaga aagaaaggag
      421 gaaatctatt gtttgaaaaa cttagacagt ctaaactaat gttttggtcg cttgaattat
      481 tgatcatagc tgcattgatc atgatttcat cagaaataaa ctttattttc gaaccgatcg
      541 ggacattttt ttccacgtta tttgcacctg tactgattgc aggctttctt tactatttat
      601 tgaatcctat tgtcaagctg ttgatgaaag caaaaatcaa aagaatatgg gctgtcgcaa
      661 ttgtgctgct ccttttagtt gcagcaatta tttggatttt acttagtgtc atcccaagtc
      721 tagtacagca aatttcctca ttggcatcta atatgcctga tttcatcaaa caggtagaaa
      781 gctggttaaa agaggtagcc gaattgcctt tgttcaagga agtagatatc aataaatatt
      841 ttgagcaatt agacatttct tacgggacaa tcattcagca attccttagt ggagtatcca
      901 atagtttagg ttcgatcgtt ggaacgatcg cttcggcaac cgtagtaatc attacagcac
      961 cattcatctt gttctatatg ttgaaagacg gagaaaaact agtgccaaac atccaacagt
     1021 ttttcccaga aaaaagaaga aaccaaattg tggagttatt ggggcaactg aataaaacat
     1081 tggctaatta cattagcgga caagcaatcg aatgtttatt cgttggtacc ttcacatttt
     1141 taggatatat ggtaattggt gtggattatg ctttcttatt tggggtcatt gcgggattga
     1201 caaacctaat tccatattta ggaccttatc taggactagc acctgcattt ctagtaacag
     1261 tcttcaatga tccggtaaaa gcattacttt gttgtgtggt tgtgctaatc gtccaacagt
     1321 tggatgggaa tatcatttat cctaatgtaa ttgggaaatc actgaaaatc catccattaa
     1381 cgatcattat cgttcttcta gtagctggaa atattgccgg attgcttggt atcttcttag
     1441 gtgttccttt ttacgcaatc tgcaagacga tcatttccta tataatcaaa atcgtcaaag
     1501 aagacaagca aaatgaaaac aagaagaaaa ttgctacgac ctcgtaggta gttttagtac
     1561 acttgttttt attgcaaaga aaattgcttt gtgataccat tttaacgtgg ctatagtgcc
     1621 acgttttttt ctatcgtaaa ctaaaaatta attttataag gagagaatgc gattatgaat
     1681 gctgaccctg agagtcagtc gctgttagcg cagattttat tattgatcgt tttgacattg
     1741 atcaatgctt ttttagcagc ttccgaaatt gctgttgttt cgatcaataa gaatcgaatc
     1801 gagcaaaaag ctgatgaagg agatgtgaaa tctcaaaagc ttttaaaaat attgcagaat
     1861 ccaaataatt tcctatcgac aatccaagta ggaatcactt tggtcaacat tttgtctggt
     1921 gcttcactag ctaatacttt atcggaacgg ttagctccgg tattaggtgg cggcacagcg
     1981 gcaaaaaata ttgcaaatat cattgtgctg gctattttga cctatgtatc gattgttttt
     2041 ggagaattgt accctaaacg tattgcttta aataaatcag aagaagtcgc aaagttcact
     2101 tcagggctga tacgcttgat tggtgtagta gctaagccct tcgtctggct gctttctgct
     2161 tccacaagtt tattggcgcg tatcacgccg atgacttttg atgatgaaga ttcaaaaatg
     2221 accagagatg aaatgcgtta tatgcttgaa aacgaaggcg tattgaataa tgaagaatta
     2281 gaaatgctcc aaggggtttt ttctcttgat acgaaagttg cccgagaagt catggtccca
     2341 cgaacggatg catttatgat tgatatcaac gacactgtgg aagaaaatgt gaatgaagtt
     2401 ttatccgaaa actattcaag aatccctgtt tataacgaag acaaagataa agtagtcgga
     2461 atccttcata caaagaattt actaaaagct gcccataaat tcggctttga taatttagat
     2521 atcaagaaaa tcatgcagga gcctttattc gttcctgaaa cggtctttat tgatgatctg
     2581 ttatatgaaa tgaaaaagac gcagaatcag atggctattt tattagatga atatggcggc
     2641 gtcgttgggt tagcaacact agaagacctt ttagaagaaa tcgtcggaga aatcgatgat
     2701 gaatcagatg aagttgagaa tctttatgaa aaaatcgatg aacacgaata tatcatccaa
     2761 ggacgtatgc tgattgatga gttcaatgaa gcttttgaca gtgatctgca tatgagtgat
     2821 gtagatacga tggctggtta tttgattaca gcattaggca tgattccgga tgaaggcgaa
     2881 aagttatcgt ttgatgtcga taatattacg ctggtttctg aagaaatgga aggttctcga
     2941 atccttaaaa tccgtgtcat ttttcatgat ccggaagaaa cagaagcgga acctgaagaa
     3001 gaacgaagat acttcagaaa agaattcgaa gatgacgagc caagaagata attggaaact
     3061 tggttatgaa agacgtgttg cttgaataaa caagcaacac gtcttttctt ttagtcagct
     3121 gacaaaaaaa tatttgactc ttatggtttg gtggcttttt cattcgctga atccatgcta
     3181 tactgattca gttgtagtag ttgacaaagg ttgtgaaaaa gctatcctgc attaagtaat
     3241 aagagcagcc aagcaaaaac agcttttcgt atttttggct tgggtgggct tattgctgat
     3301 aggctgtctc tagaacgccg tttactcgga aaaagagact gctacacggc ttttgtcaca
     3361 gccctatatg aaattgtctt tctttaagag atcggacaat ctgtgcctag agaaaatata
     3421 aataattgaa tggaagaaat ggagttaggt tatgaataat ccaaaattaa agcaacaagt
     3481 ggacaatcgc agaacttttg cgatcatttc ccatccggat gctgggaaaa caacgattac
     3541 agaacaatta ctattgtttg gtggggctat ccgtcaagcc gggaccgtaa aaggcaaaaa
     3601 aacaggtaat tttgccaaat cagactggat ggaaatcgag aaacaacgtg ggatttctgt
     3661 tactagttct gttatgcagt ttgattatca agggaaacga gtaaatatct tggatacacc
     3721 aggacatgaa gatttttctg aagatactta tcgtactttg atggccgtgg atagtgcggt
     3781 catggtaatc gatagtgcaa aagggatcga agcacagaca aagaaattat tccaagttgt
     3841 aaaaaaacgt ggcatcccga tatttacttt tatcaacaaa ctagatcgtg atggtcgtga
     3901 accattggaa ttgttagaag aattagaaga gctattagat atcgaatctt atccgatgaa
     3961 ttggccaatc ggtatgggta aagggctaga aggtctttat gatatctacc ataatcgtgt
     4021 agagttttat cgtccggaaa attatcagga tgaacgatta gtagaattgg atgaagatgg
     4081 actgcttccc gaaaatcatc ctctaactga aaattcctta tatgaacaag tcttagaaga
     4141 agtagaacta atcaaagagg ctggagatgc atttaatcaa gaaaaaattg ctcgtggcga
     4201 tcagacacca gtattttttg gttctgcatt gactaacttc ggtgtacaga cttttttaga
     4261 gactttcgtt gaattggcac cagctcctta tggacacaaa acacaagatg atcagatggt
     4321 aagtccttat gaggaagaat tttcaggttt cgtattcaag atccaagcaa atatgaatcc
     4381 agcccatcgt gaccgaatcg cttttgtccg aatctgttct ggaacattcg aacgaggaat
     4441 ggatgtctgg ttggaacgta caaataaaaa actgaaatta agcaatgtca cacaatttat
     4501 ggcagattcg agagaaaacg tcgaaaaagc agtagcaggc gatatcatcg gtgtatatga
     4561 tacaggcaat tatcagattg gggatacatt gttcgaagga aaactaaaag ttgcttatga
     4621 agaactccct tcatttactc cagagctgtt tatgaaagtg actgcaaaaa atgtcatgaa
     4681 acaaaaatcc ttccacaaag gaatctatca attagttcaa gaaggagcga ttcagcttta
     4741 taaaacatat ctaacggaag aatatatcat tggagctgtt ggtcagctac aattcgaagt
     4801 attccaatat cgtatgttaa atgagtataa cgccgaagta atcatgtctc caatgggaca
     4861 caaaatcgct cgctggatcg atccagaaga tttggatgag aagatgagtt caagtcgcaa
     4921 tatcttggct cgtgaccgtt tcgaccaacc gttgttctta ttcgagaacc aatttgcaga
     4981 acgctggttt gcggataaat atcccaatgt aaaattaaaa agtttgatgt aaagaagtct
     5041 tgacacaagc tgccctgcat caagtaataa gaatagccaa acaaaaacag cctctcatat
     5101 tttgtttaga gccgtgacgg attttccgtg acggctcttt ttactatatt aaaatcatag
     5161 aaatgtctaa tcaaaaaaat ataaaaatta ttttgcgtac atatgattaa agaaaggaga
     5221 gtgtgacaca tggaaacaga atggcaaaga cgtgaaagaa aaaaacagaa aaagaagaga
     5281 tgttacgatt atgtcgatta ttagatgtaa aagactggga caaaaacgag caaattgcac
     5341 aaaaagtacg atcaatcatc cataaagagg aaaaagttaa aaataaagac acgcgtagaa
     5401 ctgaaaatga aaagagaaaa gtacaaaaga aaagagaaaa atcaaaaagc tagaaagaaa
     5461 aagaggctga gacagaagcg tttaactcca agaaataagc tggaattcac aaaaatttga
     5521 aaagcaattt tcgtgaattt cagcttattt ccgaaggagt tgcttctgct ttcgccgttt
     5581 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtcccacgc tctttaatta
     5641 ttatgttcaa aatttgttta cacggctaat gtagcgccta attcatgttt tggaatcgag
     5701 atttcaacaa ggcctgccaa ttcgattact ttgatttcat cctcgtggta aacggcgtct
     5761 ttgattttat ctaagaaata atttttagca tcttcgattt ctttcggctg gattttacgt
     5821 ttgtcattga tcagcagaat ttcattatgt tcaggggctt cagtgtagac aatagtggta
     5881 tttccggctg tgtagacctt gactagatgg gcatcagtgt tttcaagctg gtttaataca
     5941 agacgggaat ggctgtttgt tacatttacc aatttcatgg cgctcacctc ttttatgtat
     6001 ttttaattgc ttgtttctct tattataatt tcttttttat aaaatgaaca gtattttgac
     6061 ttttctgaaa aaagaatgag acggcatttc tcaaagaaat accgtctcac gctataaaac
     6121 tggatttatt cagccgtttc ttcggctgat tctttagcca aacgttctgg tttagaagta
     6181 acgatgattt ttccatcttt atccagtttt gctttcaact catggataga agggtgatca
     6241 agataaaatt cagcgatacc atcttcgatt tgttcttgga tcgtgcgtcg aagaggacga
     6301 gcacccatag atggatcata gcctaagtca actaattttt ctttgacatt cgtaggaact
     6361 tcgatgtgaa gttgctgtgc tgccagcatt tggttgacat cgtctagcat tagtgtgacg
     6421 attgtcatca agttttcttt tgataatgca ctgaattcga tgatcccgtc aaaacggttc
     6481 agaaattctg gtgtaaagta gttgtttaat tggttcaata cagaacgagt aacaccttcg
     6541 cgtgccgcac caaaaccaac gtttgcttct actttaccag ttccggcatt acttgtcata
     6601 atgatgatcg tatctttaaa gctgaccgtt cttccttgtg catctgtcaa tcgaccatca
     6661 tctaagattt gcaagaacat atgcaaaaca tctggatgag ctttttctac ttcatctaac
     6721 aagacaagac tgtaaggatt tctccgtact ttttctgtta attgaccagc ttcttcgtat
     6781 ccgacataac ctggaggaga accaatcaat ttagagacac tatgtttttc catatattca
     6841 gacatatcga aacgaatcat tgaatcttgt gaaccgaata gttcgtaggc aagttgtttc
     6901 gcaagctctg ttttacctac accagttggt ccaacaaata agaaggagcc gattggacga
     6961 ttctgtttgt ttaatccaac acggtttcgg cgaatcgctt tcgctactcg atctacagcg
     7021 ttatcttgtc cgatgacatg agctttcaag tcatctgcta agtttttcag ttgtgtctgt
     7081 tctttttctt ttaattcacc cactggaata cctgtccgtt gttcaacgat tttttccata
     7141 tctttttcgg taatcacagg tgtttcctct tcagtgagct gacgttcttt cattttttga
     7201 agtttattga tctgatcgcg ataataagct gctttttcga aatcttcttc tcgagaagct
     7261 aaaacttttt gttcttctgc ttcttgcagt tttttctcga ttgttttagg atcaacgatt
     7321 tggatcgtca agtttttctt agaacctgtt tcatctagca aatcgatggc tttatccggc
     7381 aagaagcgat cttggacgta acgattggat aaagttgcgg ctgcttcgat ggcctcatca
     7441 gtatatttta catgatgata gtcttcataa cgtttttgca aacctttcaa aatactgatc
     7501 gtttcttcga cagttggttc atctacccgt acaggttgca tacgacgttc taaagcagcg
     7561 tctttttcga tgatgcggta ctcatttaag gtagtagcac caaccatttg caattctcct
     7621 cgagctaaag ctggttttaa aatattacca gcatccatat ttccatcacc agcagaacca
     7681 gcaccgacga tttcgtgaac ttcatctatg aataggatca cattttctgc ttgacggatc
     7741 tcttcaatca atttttgcat acgttcttca aattgtcctc gtataccagt accttggact
     7801 aaagatacga catctaaacg aatgacttct ttgtccatta atttttgcgg tacatcacca
     7861 tctacgattt tttgtgctaa accttcaaca actgctgttt taccaacacc tggttctcca
     7921 atcaagacag gattgttctt ggttcggcga tttaaaattt caatgactcg tttgatttcg
     7981 tcatcgcgtc cgatcacagg atcgatatca ccttgtcgtg cttgttctgt gatattgatt
     8041 ccgtattctc ccaataaacc ttctgtattg ccttgaggtg gctgaccacc attgaagttg
     8101 ttgtttccgc caaattgtgt tggtggtgtt tgctcataat ttccttgctg ttgcatttgg
     8161 cgagacatgg aacggaaaag atcgtccaaa ctgccaaatc caaatggatc attttgggcc
     8221 atattagtca gacctccatt ttgttgattt ttgatttttt gataacagct ctgacagtaa
     8281 tcgagttgtg tccgctgacc attgacagtg gcatacaagt gaatcgttgc ctcattcttg
     8341 ccgcagtttt gacaaagcat gaatcattca cgtcctttca caaaaatggg tacatccata
     8401 ttttacatct cgtttttcat gaggtaaaag aataacccct agttttttaa gagatttatg
     8461 aggtagaaaa ggtgaaatct ctattgacca ttcctgacct ttgactctta ttatactaaa
     8521 aataaaaagg gaagcaagta attcgtttca tatgaaaaca aagagggtca aaggaaagaa
     8581 tgataaacgt caggagaaga gaaagcgaca agaaaaagga aataaacacg tttgttataa
     8641 aatgtgaaga aaaggatgaa aaagaatccg gaataatttg aatactttta tctgaaattg
     8701 aatgaaaact gttgtatcta acgaaaaaaa atgttaatct ttacagatga aagggtggca
     8761 tttgttagga ttctctgaat tactaaaaaa gaaaaccctc acataaaaac aacactcact
     8821 taaaggagac cgattaatat ggaaaagaaa gaatttcacg tagtagcaga aactgggatc
     8881 cacgctcgtc cagctacact attagtacaa acagcaagca aatttaactc tgatgtaaac
     8941 ttagagtata aaggtaaatc agtaaacttg aaatcaatca tgggcgttat gtctttaggt
     9001 gtaggtcaag gttctgatgt tactattact gcagaaggtg ctgacgaagc tgatgcaatg
     9061 gcagcaatcg ttgaaacaat gaagaaagaa ggcttatctg aataatggtt gaaatgctaa
     9121 aagggattgc cgctagtgac ggtgtagccg ttgcaaaagc ttacctgcta gttcaaccgg
     9181 atttaacatt cagtaaggca acagtagaag atactgcggc tgaagaagct cgtttggatg
     9241 ctgcattagc taaatctact gaagaattgc agcaaattcg tgaaaaagca gcgcaaagct
     9301 taggtgaagc agaagcgcaa gtatttgatg cccatttgat ggttctttca gatccagaaa
     9361 tggtaggtca aatcaagcaa aatattaaag acaacagcgt aaatgctgaa tcagcgctaa
     9421 aagaagtaac ggatatgtat atcggtatgt tcgaagcaat ggaagataat gcttatatgc
     9481 aagagcgtgc tgcagatatt cgtgacgttg ctaaacgtat tttagcgcac ttattaggcg
     9541 ttactttacc aaatccatca atgatcaacg aagaagtggt cgttgttgca catgacttga
     9601 ctccaagtga tacagcacaa ttagatcgca attttgtaaa agcgtttgta acagacattg
     9661 gtggacgtac ttcacattct gctatcatgg cacgttcttt agaaattcca gcaatcgttg
     9721 ggacaaaaga aattactgct aaagtcaaag aaggcgatat tttggctgtc aacgggatcg
     9781 aaggcgatgt aatcatcgat cctacagatg aacaaaaagc tgaatttgaa aaagcaggtg
     9841 cagattacgc agcacaaaaa gcagaatggg aaaaattaaa aaatgcagaa actgtcacag
     9901 ctgacggcaa gcattttgaa ttagctgcaa acatcggaac accaaaagat ctagttggtg
     9961 tccataataa tggtggagaa gcagttggtc tgtaccgtac tgaattcctg tatatggatt
    10021 ctccagactt cccaacagaa gatgaccaat atgaagctta caaagctgta ttagaaggta
    10081 tggaaggaaa acctgtagtt gttcgtacaa tggacatcgg tggagataaa gaactaccat
    10141 atctgcaatt acctcatgaa atgaatccat tcttaggata tcgtgcattg cgtatcagtt
    10201 tatctgaaca aggcgacgaa atgttccgta cacaaatgcg tgcattgctt cgtgcatctg
    10261 ttcatggtaa tttacgtatc atgttcccta tggtagctac actcaaagaa ttccgcgcag
    10321 caaaagcgat ctttgaagaa gaaaaacaaa aattgataag tgaaggcaag gaagtatctg
    10381 atacgatcca agtcggcatc atgatcgaga ttccagcagc agcagtctta gcagacaagt
    10441 ttgcaaaaga agtagatttc ttctctgtag gtacgaacga tttgattcaa tacacaatgg
    10501 cagcagaccg tatgaacgaa cgcgtttctt acttgtacca accatataac ccatcaattt
    10561 tacgtttgat caaaaatgta attgatgcag cacacgctga aggaaaatgg gcaggtatgt
    10621 gtggagaaat ggctggagat caaacagctg ttccattgct agtaggtatg ggtctagatg
    10681 agttctcaat gagtgcgaca tctattctta aaacgcgtag tttaatgaaa cgcttggaca
    10741 cagcgaaaat ggctgaactt gctgatcgtg cattgaaaga atgcgatatg atggaagaag
    10801 tcgttgaact tgttcatgaa tatgtaaaat aaacaacgta tcaaaagctg gtgtggcttg
    10861 taaaagagct acgccagttt ttttgttctt ttttaagaaa acgtttttgt cagtaagaat
    10921 agacaaaaat
//
LOCUS       DARBEC010000092        10848 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.096, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000092 DARBEC010000000
VERSION     DARBEC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10848)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10848)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10848
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.096"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(64..486)
                     /locus_tag="SCL80_002327"
     CDS             complement(64..486)
                     /locus_tag="SCL80_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1108170.1"
                     /translation="MRNKKVIVRILIVSFLLFGCFKLIKTNEESNGKLINSNLEEVLE
                     LQKTNQSFFVYLARNDCKKCQEVESFLERYDKKFSRNVYRIETRKEPRQEELQTFLQR
                     YHVKSVPSFLVIHDRNVKKIERKTFFFEKTLNQFLPNF"
     gene            complement(476..2650)
                     /locus_tag="SCL80_002328"
     CDS             complement(476..2650)
                     /locus_tag="SCL80_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 family protein"
                     /protein_id="HEG1108171.1"
                     /translation="MMNAPKRPTHFEQMFSVDRKRKQEKETPKSRFQQLYFIVTLLGV
                     CLYPFFIVGSIPLAIVFMIDKKETRAHIYDFEYESFLKRRSSLFFIFALGFTLLNLFC
                     FIFVLPRGYLSCYLLFPLNLFSHALQFNGQTIFALLLGGTGAGSCLIAYASFIEKRKV
                     VSKKERQTALLNSKEYKERRKNKFQESQKYTAEYQQAYQVAIVIKDVALRNKQLASLA
                     KVILLGTDEYGLPYVMNFSELNQHVIIPATTGSGKTTVIQLLAEHACKFRIPVILVDG
                     KGAGETLQALKEVAEAYNRPIEVFVDGGDLRYNPVKNGNDIAIRDKLTNLAETESVYY
                     STAAKALLQVTIQLLDEFQKSHKIERNLVWLQHYLLPRNVLHLFADRIQLKNPDLFKE
                     KVVVEKKKTTKKKTKKNEAMDKTERDFADVEQLSEERIVEHDRKTVDLETYYLLLKEN
                     ISLMTEIEQTLFKRLFVRYEHKKNPFYLYATSENLQTNINMLLDSELGELFDTSGGKR
                     ELDIQEVVKEGKIVYISLNGLIYSEFIRTLAQMLVGDINFFASEVYKQAGETANRSKV
                     LVIFDEPASYLNDNLIDLVNKGRGAGVHAAYAPQTLADIDRIDPVLRKQLIGNVNTFF
                     IGKTNDPTEIDYWADLMGTEEDIELTEMTTQEAGYSDMGKVDWTGERGTKRDVDRFKF
                     NPNRIRALRTGEFVVYRTAEDVQEPPRVVYIRKPNLEKQYAK"
     gene            complement(2666..5008)
                     /locus_tag="SCL80_002329"
     CDS             complement(2666..5008)
                     /locus_tag="SCL80_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108172.1"
                     /translation="MRKKSLKWWTLLLLGGLFFCLQGATVSADQMNDGAGVTETDRSE
                     GENANDKVEVKEVTTDVDQLYKNYKKNSFELMTNDYESMNLKKKLSYNVAGVMKNFVW
                     SGVKMLGEFNASMVEFLFELDIISAIREPIQNLTQKIADNMLSIAGTIGISFSAIMMA
                     IKYAGEARMKQALRVFLMTILIFTGLSVLKDSGTNKNLFDVLFSLDSTVETAFVKVNP
                     VLTPEEETNYETKETEEVTADKKVETAGKLMAARVFYSNVYEPYLLLNYGTTNVDTIR
                     EKKVKYAGKEYDRINLLLDNDLGTKIGEKIHDKVTEYEADTLKNKNIQYYNSWKNTAF
                     ALFYFVVNLIQTVVYFVLSMFRLIIGTMQLLLVPMLPFLLFLGLFLVTMNVFGNYAKV
                     FGILTFLKGMTGFACILFSSFLSYGFQLSSTQDDPWEKILLIIVYLFAPLGIYLFRGF
                     LGSLFTGRMGVRGALAFAGNPFGAEAKLRREAKQRLKERKEARKAQKQKEKENARKRQ
                     ERQRKRGGGSDVRMPQTAKQATRSAFRRESAQQPRHKAPNRMERMAQKARDLHDQAKQ
                     AEEQSQERMQRTAMEKQARKTADLLQRELQGNPSLRERSRKGASRSPYRNQGSAMVVS
                     LANPKQKHKSYGQKHTPRTELNDRKRRQGQTSRRRKEALTPVTKTSSMKTRTVAGQKH
                     AVQKPLRKGNVPRATGSTTPKSVRRPEGVREHMSAVNRVTAQTKAPSTVSKAYSTMKQ
                     TKSPKQAPKTRSEFRATQAKMKRKREKQPIYRREMKQRKR"
     gene            complement(5030..7573)
                     /locus_tag="SCL80_002330"
     CDS             complement(5030..7573)
                     /locus_tag="SCL80_002330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1108173.1"
                     /translation="MAFLFLLVVFIIGIWQFKKKKTKLPRLEFPIDAIIRNFVLTKND
                     DVWVGYQLSSQVFPLNDIDFFKNYLQDGEGILGHDEYDYHFINIPRQFNLDTHIEETI
                     RDLVKGECADLGKVYFEQAGKILKDEVQLNEYRTYLFVRFTTEMKVSDPIEYFQLLKH
                     VFKKFIYRITGQRVPISSVLPTYETLEKELYTELSNYKPIERLSAQEIGQIYYYFFHR
                     SEEKLPERQLTPMEICEGVISNEAGYIAIEQLNQTHYSTFLSQTDAPLAMYGSSFIQN
                     LQDSLSFPIDTHIRLRFEHSKSDERKINKMAGRIYEQEKEYETVDGILDSDDVLNFGT
                     ERLMDVKDKLKRKEKRLCYSTVTLVVSASSKEELEERVKDIEFAIESTDFKFYRSVAD
                     QLTLFNQCLIGSTQRFRSYEQVATTGFVADYGLDLKKEVGNKKGFPLGRIVIAKKFKS
                     AKEALALSSKLVWYYPNLTKKSIEGAEHTNGNTLIIGPPGKGKSVLVKYIFLWLMFLG
                     QKILYIDPKNETQKFFLKALEKFGHIPEFVAMYERINFISLSSDESCRGMLDPLLFLP
                     KEEAIQTAKNVLGMLGEVDTNRETASHKKTVIQDAIDVVYTMPGKHSLSQVIEEIRKS
                     DKELAKLILGHSVGLGKILIGNEHSTPIRFDSQINVLGTQGIRIPTQKEIDSGRLNSE
                     QLAGMAIMEVIMKFTYIFSTNKEEDAAIIYDEAKGYEDTAQGSYLIDDSLRKGRANNT
                     DIYLVVQAFKDYDQEDKKELISYKFAFRPKQKEAREKLLSFFDMEVNSANLEMMEQLQ
                     TGTCLFQDHLGRNQPIAIDVLFDSWLLAVSSTENQDEKIQAMLENEKQR"
     gene            complement(7583..7981)
                     /locus_tag="SCL80_002331"
     CDS             complement(7583..7981)
                     /locus_tag="SCL80_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108174.1"
                     /translation="MEYNYTKEFKHPHKIYGIKGQKIPWISNGIRMEQLVIGGVFLFI
                     LLILFLIAYVNQISFLQTLFRKSWLIMIFGIGVLVWTLFSMKWDNKHFFEYLIGRTSY
                     YQKRKQRFEHEMYVPFFHEKVTYSLHKWKE"
     gene            complement(8062..8346)
                     /locus_tag="SCL80_002332"
     CDS             complement(8062..8346)
                     /locus_tag="SCL80_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108175.1"
                     /translation="MKLFDLKSLFDDVSGQVIWLLAIVAIVLMVGAYATQGFARAAFA
                     IIGVFILIMLVLLLSNAVDIGEWLKNIIFIKDSEENVGVTQMIVPWIQLS"
     gene            complement(8370..9368)
                     /locus_tag="SCL80_002333"
     CDS             complement(8370..9368)
                     /locus_tag="SCL80_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108176.1"
                     /translation="MSNAKFNPQAFQTKPPKQQKEKKQKKRFLFQKKKTEPSDRMIQF
                     SQKSAMSVFSGAFFCLFLLMGVVLLLNFGRVDTLTRLAVKKQVNPETLLTEVNQTLSS
                     TEQLKYEGQHFIERLFTYSPEKRNEWEASVNHYLAQGLEASDLGFVTTATNRVAKDVR
                     FIKLEEIDLTQAMYRLFYEVTFTEGEHWKSSQLSFPVSYQNNVLKVLDGVQFLNLSES
                     KNTNSVNYQENRFYSSGQAVTESEEIKLEAFVKRFFELYVKNDEKLALISNEKGLEGG
                     ELINVQLDHAVKNKEGNYFLQGKYRFYFEKGNEFQSNFSMEVKTSGDSYFVTTLNE"
     gene            9507..10846
                     /locus_tag="SCL80_002334"
     CDS             join(9507..9948,9948..10846)
                     /locus_tag="SCL80_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEG1108177.1"
                     /translation="MRKYTFVFKKKVVSDYLNNEGGYKYLAHKYQINRTLVRHWVRIY
                     NYHGWEGLVGGGKSYTTKFKLDVIEYMETNGLSIQETAKKFNIGSNRTLSKWIEQYEE
                     GGASSLESQKRGRKISMNSKLNIPKKLKDESLEEEVIRLRAENAYFKKVRNLDSRTRF
                     EQEEIKVKTILQLKKEFKLNLLLSIAQLAKSTYYYWVKKLDKPDKYSKIKQEITAIVK
                     ESRNSYGYRRVTLALKMKGYTINHKTVRKLMSQMGLTCQIRIKRYKSYKGTVGKIAKN
                     VLKRNFSVDTPNKKWVTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEE
                     MLQQAIKKKGTEGSLILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENF
                     FGVLKSEFFYREKFRSIEIFQSKLNEYIRWYNNKRIKLKLNGLSPVEYRKQSIK"
BASE COUNT         3280 a         2199 c         1715 g         3654 t
ORIGIN      
        1 cgaaaaaaat ttgaactcat aacaattttt tgtttttttc aacgtattct ctttcaattt
       61 tcactaaaaa ttaggaagaa attgattcaa tgtcttttca aaaaagaatg tttttctctc
      121 aatttttttg acgtttctat catgaatcac gagaaaactt gggacacttt taacatggta
      181 acgttgtaaa aaagtttgaa gttcttcttg tcttggttct tttcttgttt caattcgata
      241 tacattccta gagaattttt tgtcgtatct ttccaagaaa gattcaactt cttggcattt
      301 tttacaatca tttcttgcta gatacacaaa aaatgattga ttcgtttttt gaagttctag
      361 tacttcttct aaattactat tgattagttt tccatttgat tcttcgttag tttttatcag
      421 tttaaaacaa ccaaataaga ggaaactaac aataagtatc ctaacaataa ctttcttatt
      481 tcgcatattg tttctccaaa tttggtttcc gaatatagac gactcgcggt ggttcctgga
      541 cgtcttcagc tgttcgatac accacaaatt cgcctgttct caatgctcga atgcgattcg
      601 gattgaattt aaagcggtct acgtctcgtt tcgtgcctct ttctcctgtc caatcaacct
      661 ttcccatatc agaataacct gcttcttgtg tcgtcatttc cgttaattca atgtcttctt
      721 ctgtccccat taaatctgcc caataatcaa tttctgtcgg gtcatttgtc ttcccaataa
      781 agaacgtatt cacattccca atcaactgtt ttcgaaggac aggatcaatt ctatcaatgt
      841 ccgcaagcgt ttgaggggca tacgccgcat gcaccccagc gcctcgtccc ttattgacta
      901 agtctataag gttatcattt agataagaag ctggttcgtc aaatattact aggactttcg
      961 agcgattggc tgtttctcct gcttgtttat acacttctga cgcaaagaaa ttaatatctc
     1021 ccacaagcat ttgagccaat gtccgaataa actcactata aatcaatccg tttaacgaga
     1081 tataaacaat ctttccttct ttaacaactt cttgaatatc taattctctt tttcctccag
     1141 aagtatcaaa gagttcacca agctcactat ccaataacat attgatattg gtctgtaaat
     1201 tctcagaagt cgcatacaaa taaaacgggt tcttcttatg ttcgtaacga acaaacaacc
     1261 gtttaaacaa cgtttgctcg atttctgtca tgagagaaat gttttctttt aataatagat
     1321 aatacgtttc taagtcaacg gttttacgat catgttcaac gattcgctct tcacttaact
     1381 gttccacatc ggcaaaatca cgttctgttt tgtccatcgc ttcattcttt ttcgtttttt
     1441 tcttcgtggt tttcttcttt tctacgacca ctttttcttt aaataaatct ggatttttca
     1501 actgaatacg atccgcaaac aaatgtaaga cgtttcgtgg caataaataa tgttgtaacc
     1561 aaactagatt tctttcaatt ttatggctct tttggaactc gtctaaaagc tgaatcgtta
     1621 cctgtaataa cgctttggct gctgtcgaat aatacacgct ttctgtttct gctagattgg
     1681 ttaatttatc tcgaatggca atatcattgc cattttttac aggattataa cgcaaatcgc
     1741 caccgtccac aaagacttca atcgggcgat tgtatgcctc tgccacttct tttaacgctt
     1801 gtaaagtctc tcctgctcct ttcccatcga ctaagataac ggggatacga aacttacacg
     1861 catgttctgc tagtaattgg atcaccgtcg tttttcctga acctgtagtc gctggaataa
     1921 tcacatgttg gttcaattca ctaaagttca tcacataagg gagcccatac tcatctgttc
     1981 ctaatagaat cactttggct aatgaagcca gttgtttatt ccgcaaagca acgtctttta
     2041 tgacaattgc tacctggtac gcttgctgat actctgctgt atatttttgg gactcctgaa
     2101 atttattctt ccgacgttcc ttgtattctt tactatttag caaagctgtt tgccgttctt
     2161 tctttgaaac aacttttctt ttttcaataa aagacgcata cgcaatcaaa caacttcctg
     2221 cacctgtccc acctaaaagg agggcaaaaa tcgtttgtcc attaaactgc aacgcatgag
     2281 aaaacaagtt taatggaaat aataagtaac acgacaaata ccctctagga agaacaaaaa
     2341 taaaacaaaa tagatttaat agcgtaaaac ctaacgcaaa aataaaaaaa agagagcttc
     2401 ttcgttttaa gaagctctca tactcaaagt cataaatatg tgcacgtgtc tcttttttat
     2461 ctatcataaa gacaatcgct aaaggaatac tgcctacaat aaaaaatgga tatagacaaa
     2521 cacctaataa agtgacgata aaataaagtt gttgaaaccg actttttggc gtttcttttt
     2581 cttgttttct tttgcgatcg acagaaaaca tttgctcaaa atgtgtcggt cgttttggtg
     2641 cattcatcat actatcgatt tcctcctatc gttttcgttg tttcatttct cttcgataaa
     2701 tcggttgttt ttctcgtttt cgtttcattt ttgcttgagt tgctcgaaac tcactccgtg
     2761 tcttcggtgc ttgttttggt gattttgttt gtttcatggt tgagtatgct ttagatacgg
     2821 ttgaaggcgc tttggtttgt gctgttactc gattgactgc tgacatatgt tcccgaaccc
     2881 cctctggacg tcgaacagat ttcggtgtgg tagaacctgt tgctcgtggt acattccctt
     2941 ttcttagtgg tttttgtacc gcatgttttt gtcctgcaac tgtccgggtt ttcatcgaac
     3001 ttgtttttgt tacgggagta agtgcctcct tccgacgacg acttgtttgt ccctgacgac
     3061 gttttcgatc gttcaattct gttctaggcg tatgcttctg gccataagac ttgtgttttt
     3121 gctttggatt cgctaatgac accaccattg cagaaccctg attgcgatac ggactacgag
     3181 acgcaccttt tctacttctt tctcttagac taggattgcc ttgcaattct cgttgtaata
     3241 aatcggctgt ttttcttgcc tgtttctcca ttgctgttcg ttgcatacgt tcttgacttt
     3301 gctcttctgc ttgtttcgct tgatcatgca agtctcttgc tttttgtgcc atacgttcca
     3361 tacgatttgg tgctttatga cgaggttgtt gtgcggattc acgccgaaaa gcacttcgtg
     3421 tggcttgttt cgccgtttgt ggcatacgaa cgtctgagcc acccccacgt tttcgttgac
     3481 gttcttgtct cttccgtgcg ttttcttttt ctttttgttt ctgtgctttt ctcgcttctt
     3541 tgcgttcttt caaccgttgt ttggcttccc ttctcaattt tgcttctgcc ccaaatggat
     3601 ttcccgcaaa agctaacgct cctcgaacac ccattcttcc agtaaataga ctgcctaaaa
     3661 agcctcggaa caaataaatt cccaacggcg caaacaaata aacaatgatc aagagaattt
     3721 tttcccatgg atcgtcttgt gtactactta attggaaccc gtaactaaga aaagaagaaa
     3781 aaaggataca tgcaaaacct gtcattcctt ttaaaaacgt caaaatacca aaaactttgg
     3841 catagttacc aaacacattc attgtgacaa gaaacaatcc taaaaaaagt aagaacggca
     3901 acatcggtac taacaatagt tgcatagttc caataatcaa tcgaaacata cttaagacaa
     3961 aataaacaac ggtttgaatc agattcacga caaaataaaa caaggcaaat gctgtatttt
     4021 tccaactatt gtaatattgt atgttcttgt ttttcagtgt atctgcttca tactctgtga
     4081 ctttgtcatg aattttctcc cctatttttg tacctaaatc attgtccaat aaaagattga
     4141 ttcgatcata ttcttttcca gcatatttga ctttcttctc acgaattgtg tctacattgg
     4201 ttgtgccata atttaacaac agatatggtt cataaacatt actataaaag acccgagccg
     4261 ccatcaattt gcctgccgtt tccacttttt tatccgctgt tacctcttct gtttcttttg
     4321 tttcataatt tgtttcttct tcaggtgtta acactggatt gaccttcacg aacgccgttt
     4381 ccactgtaga atctaaagaa aaaagaacat caaaaagatt cttatttgtc ccagaatctt
     4441 ttaatacgct taacccagta aaaataagga tcgtcataag aaagacacgg agtgcttgtt
     4501 tcattcgagc ctctccagca tatttgattg ccatcataat tgccgaaaaa ctgattccaa
     4561 tagttcctgc aatacttaac atattgtccg caattttttg tgttaagttt tgaatgggtt
     4621 ctcgaatggc ggaaataata tctaattcaa agagaaattc aaccattgaa gcattgaatt
     4681 ctcctaacat ttttacgcca ctccaaacaa aatttttcat cactcctgca acattgtacg
     4741 atagcttttt tttaaggttc attgactcat agtcattggt catcaattca aacgaattct
     4801 ttttgtaatt tttatacaat tgatccacat cagtcgttac ttcttttact tctactttat
     4861 cgttggcatt ctctccttca gaacgatcgg tttcagtgac acccgcacca tcattcattt
     4921 gatcggcaga aacagtagcc ccttgtagac agaaaaaaag accccctaat aaaaggagcg
     4981 tccaccattt cagagatttt tttcgcaaaa aaaccacctc ttttttgttt tatcgttgtt
     5041 tttcattttc taacattgct tgaatttttt catcttgatt ttcagtagaa gaaacagcca
     5101 ataaccaact atcaaacaac acatcaatgg caatcggttg atttcttccc aaatgatcct
     5161 ggaataaaca ggttcctgtt tgtaattgtt ccatcatttc taaattggct gaattcactt
     5221 ccatatcaaa gaaggacaat agtttctctc gtgcttcttt ttgtttcgga cgaaacgcaa
     5281 atttgtaaga aattaactct tttttatctt cttgatcgta atctttaaac gcttgcacaa
     5341 caaggtaaat atccgtatta ttcgcacgtc cttttcgcaa gctatcatca atcaaatagc
     5401 ttccttgtgc ggtatcttca tacccttttg cctcgtcata aataatcgca gcgtcttctt
     5461 ctttgtttgt agaaaagata tacgtaaact tcataatgac ttccataatt gccattcctg
     5521 caagttgttc gctatttaat cgacccgaat caatctcttt ttgtgtcgga atgcgtattc
     5581 cttgtgtccc taacacgttg atttgtgaat caaaacgtat cggcgtactg tgctcattcc
     5641 caatgagaat ctttcctaaa ccaacgctat gccctaaaat caatttagct agttctttgt
     5701 cactttttcg aatttcttcg ataacttgac ttaacgaatg cttacctggc atggtgtaaa
     5761 ctacatcgat ggcgtcttgg atcaccgttt tcttgtggct cgctgtttct cgattggtat
     5821 ctacttctcc taacattcct aacacatttt tagcggtttg aattgcctct tcttttggta
     5881 aaaacaatag aggatcaagc atccctcgac agctttcatc tgaagataac gaaataaaat
     5941 taattcgttc atacatagcc acaaactctg gtatgtgtcc gaatttttca agtgccttta
     6001 aaaagaactt ttgggtttca tttttcggat caatataaag aattttttga cctaaaaaca
     6061 tcagccataa aaaaatatat tttacaagga ccgattttcc tttacctggt ggaccaataa
     6121 tcaacgtatt tccattggta tgttccgcgc cttcaatact tttctttgtt aaattgggat
     6181 aataccatac tagtttacta gatagcgcta acgcttcttt cgcagattta aacttttttg
     6241 caataacaat ccgtcctaaa ggaaatcctt tcttatttcc tacttctttc tttaagtcca
     6301 agccataatc tgccacaaaa cctgtcgtag cgacctgctc gtaactacga aaacgttgcg
     6361 tacttccaat taagcattga ttaaacaacg ttagctgatc cgctaccgat cgataaaatt
     6421 tgaaatctgt agactcaatc gcaaattcaa tatctttgac tcgttcttcc aattcttctt
     6481 ttgaagaagc agatacaacc agcgtgacgg tagaataaca caatcgtttt tctttgcgct
     6541 tcagcttatc ttttacatcc attaaccgtt ctgtaccaaa atttaataca tcgtctgaat
     6601 ctaaaatgcc gtcaacggtc tcatattctt tttcttgttc ataaatccgt ccagccattt
     6661 tattaatttt tcgttcatca gattttgaat gctcaaatcg aagtcgaata tgcgtatcaa
     6721 ttggaaaaga taagctatcc tgaagatttt gaataaagct actcccatac atggctaaag
     6781 gagcgtccgt ttgacttaaa aacgtcgaat aatgcgtttg attcaactgt tcaatcgcaa
     6841 tgtatcctgc ttcattcgaa atgacgccct cgcaaatttc cattggcgta agctgtcttt
     6901 caggcaattt ttcttctgat cgatggaaaa aataataata aatttgtcca atctcttggg
     6961 cagataagcg ctctatcggc ttataattgg acaattccgt atagagctcc ttttctaatg
     7021 tttcatacgt tggtaacaca ctacttatag gaactcgttg tcccgtaata cgatagataa
     7081 actttttaaa aacatgctta agcaattgaa aatattcgat cggatcagac actttcattt
     7141 ctgtagtgaa acgcacaaac agatacgttc gatactcgtt taattgcact tcgtctttta
     7201 aaatctttcc tgcctgttca aagtacactt tcccaagatc tgcgcattct ccctttacca
     7261 aatctcgaat ggtttcttca atgtgcgtat ctaggttaaa ttgacgtgga atattgataa
     7321 aatgatagtc atattcatca tgtccaagta tgccttcgcc atcttgaaga tagttcttga
     7381 aaaaatcaat gtcatttaaa ggaaatactt gggaagaaag ttgatagcct acccacacat
     7441 catcattttt ggtcaaaaca aaatttcgga taatggcgtc tatcggaaat tctaatcgag
     7501 gaagcttcgt ttttttcttt ttgaactgcc aaatcccgat aataaagacg accaaaagaa
     7561 acaaaaacgc catttactcg actcactcct tccatttgtg tagggaatac gtcacttttt
     7621 catggaaaaa cggcacatac atttcatgtt caaagcgttg tttccgtttc tgataatagg
     7681 acgttcgccc aattaaatac tcgaaaaagt gtttattgtc ccatttcatt gaaaacaatg
     7741 tccaaacgag aacaccaatc ccaaaaatca taatcaacca ggactttcga aacagtgtct
     7801 gcaagaaact aatttgattc acatacgcaa tcaaaaatag gatcaaaaga atgaagagaa
     7861 ataccccacc tatgactaat tgttccatgc gaatgccatt agaaatccag ggaatttttt
     7921 gtcctttaat cccgtaaatt ttgtgagggt gcttaaactc ctttgtatag ttgtattcca
     7981 tgttcactct acctcctttt acaaaaaaag aagcgaccag aagttctaat cttctgatcg
     8041 cttttgcagt ttgtcttttt cttatgataa ttgtatccag ggcacaatca tttgcgttac
     8101 ccccacgttc tcttcactat ccttaataaa gataatgttc tttagccatt caccaatatc
     8161 aacggcattc gataacaata agacgagcat tatcaaaata aagacgccaa taatggcaaa
     8221 cgctgcccgc gcaaagcctt gggtcgcata agcaccaacc attagaacaa tcgcaacgat
     8281 tgccaatagc caaatgactt gtccagatac atcatcaaat aacgatttaa gatcaaaaag
     8341 tttcatttcc attctcctct aagttcattt tattcattta acgtagtaac aaaataacta
     8401 tcgccacttg tttttacttc cattgaaaag tttgattgga actcgtttcc tttctcaaaa
     8461 tagaaacgat acttcccttg caaaaagtag tttccttctt tattttttac tgcatgatcc
     8521 agttgtacat tgatcaattc gccgccttct aacccttttt cgttagaaat caatgccaat
     8581 ttttcatcat ttttgacata taactcaaaa aaccgtttca caaatgcttc cagcttgatt
     8641 tcttcgcttt cagtgaccgc ttgcccagaa gaataaaatc gattttcctg atagttcaca
     8701 gaattggtat tcttactctc agaaagattc aaaaattgaa cgccatcaag tacttttaaa
     8761 acattgtttt gataactgac tggaaaagaa agttgactgc ttttccaatg ttctccttct
     8821 gtaaaagtaa cctcataaaa taggcgatac attgcttgtg tcaaatcgat ttcttctagt
     8881 ttaataaagc gaacatcttt cgctactcga ttggtagccg tcgttacaaa gcctagatca
     8941 gacgcttcca atccttgtgc taaataatga ttgacggaag cctcccattc gtttcgtttt
     9001 tctggcgaat aagtaaataa cctttcaata aaatgttgtc cctcgtactt caattgttcc
     9061 gtagaagaca aggtttggtt gacttcagtt aacaatgttt ctggattgac ttgttttttc
     9121 actgctaaac gcgttaatgt atccactcgt ccaaagttta ggaggagcac gacacccatt
     9181 aacagaaaca aacaaaaaaa agcgcctgaa aatacactca tggcactttt ttgcgaaaat
     9241 tggatcattc gatctgacgg ttctgttttc tttttttgga acaaaaaacg tttcttttgt
     9301 ttcttttcct tctgttgttt cggtggctta gtttggaacg cttgtggatt aaattttgca
     9361 ttactcatga gaaatgccat accgttctaa caataatttc ggtattttgt ccacaatcac
     9421 tacttcgtgg acaaccacac ttgccccctc caatgatgtg caccccaaaa gttagactag
     9481 aaatctaatt aaaaggggtg ctttttttgc ggaaatatac atttgttttt aagaaaaaag
     9541 tagtttcaga ctatttaaat aacgaaggcg gctacaaata tcttgcacat aaatatcaaa
     9601 taaatcgtac actggttaga cattgggtaa ggatttataa ctatcatggt tgggaaggct
     9661 tggttggagg tggcaaaagc tacactacaa aatttaaact tgatgttata gaatatatgg
     9721 aaacaaatgg tctttctatc caagaaactg ctaaaaaatt taatatcggt tcaaatagaa
     9781 ctctaagtaa atggatagag caatatgaag aaggcggtgc ttcttcactt gagagccaaa
     9841 aaaggggcag aaaaattagt atgaattcca agctaaacat tcctaaaaaa cttaaagatg
     9901 agtctcttga agaagaagtt attcgtttaa gagcagagaa cgcatattta aaaaagttag
     9961 aaaccttgat tcaagaacaa gatttgagca agaagaaatc aaggttaaaa caatccttca
    10021 gctcaaaaaa gaatttaaat tgaatctatt actttcaatt gcacaattag cgaagtcaac
    10081 ttattattac tgggtaaaaa aattagataa gccagataaa tatagcaaga taaagcaaga
    10141 aattacagcg attgtaaaag aatcaagaaa ctcttatggt tatcgtagag tcactttagc
    10201 gttaaagatg aaaggatata caatcaatca taaaacagtt agaaaattaa tgtcccaaat
    10261 gggacttacc tgtcaaatcc gaataaagcg atataaatct tataaaggaa cagtaggaaa
    10321 aattgccaag aatgtgttaa aacgaaattt ttcagtagat acacccaata aaaaatgggt
    10381 gacagacgtc actgaattca agataaaagg aagaaaaatc tatctatctc ctatacttga
    10441 tttatttaac ggagaaataa ttagttatag tatatcaact agtccaacat ataaactgat
    10501 tgaagaaatg cttcaacaag caattaaaaa aaagggaact gagggttcac tgattctaca
    10561 ttcagatcaa ggatggcagt atcagatgcc acaatatcaa aaaaaattaa aagagaataa
    10621 cattattcaa agcatgtcta gaaaaggaaa ttgcttagat aattctgtaa tagaaaattt
    10681 ttttggtgtg cttaaatcag agttttttta tcgagaaaaa ttccgatcaa ttgagatatt
    10741 tcaaagtaaa ttaaatgagt atattaggtg gtataataac aaaaggataa aactaaagtt
    10801 aaatggacta tctccagtag aatatcggaa acagtccatt aaatagtt
//
LOCUS       DARBEC010000093        10817 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.097, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000093 DARBEC010000000
VERSION     DARBEC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.097"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(25..162)
                     /locus_tag="SCL80_002335"
     CDS             complement(25..162)
                     /locus_tag="SCL80_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEG1108178.1"
                     /translation="MKRGIVMEIVKDILLVFGGSMLGVTVMCLMHASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="SCL80_002336"
     CDS             complement(159..1343)
                     /locus_tag="SCL80_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEG1108179.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPDLTKKCQKEECISLFRTFKSYRSGELLKPDDKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAYHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNQKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNQTTYIQDMVNNTRLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="SCL80_002337"
     CDS             complement(1599..1853)
                     /locus_tag="SCL80_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108180.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="SCL80_002338"
     CDS             complement(1878..3020)
                     /locus_tag="SCL80_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEG1108181.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="SCL80_002339"
     CDS             complement(3080..4432)
                     /locus_tag="SCL80_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1108182.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="SCL80_002340"
     CDS             complement(4503..4847)
                     /locus_tag="SCL80_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1108183.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDAEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="SCL80_002341"
     CDS             complement(4857..5231)
                     /locus_tag="SCL80_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1108184.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="SCL80_002342"
     CDS             complement(5244..5558)
                     /locus_tag="SCL80_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1108185.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCL80_002343"
                     /pseudo
     CDS             complement(5672..8897)
                     /gene="fss3"
                     /locus_tag="SCL80_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
     gene            complement(8976..9638)
                     /locus_tag="SCL80_002344"
     CDS             complement(8976..9638)
                     /locus_tag="SCL80_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108186.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            10255..10485
                     /locus_tag="SCL80_002345"
     CDS             10255..10485
                     /locus_tag="SCL80_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108187.1"
                     /translation="MMEDFIRKNISDEYADFYEQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETNFKNRSE"
     gene            10491..10790
                     /locus_tag="SCL80_002346"
     CDS             10491..10790
                     /locus_tag="SCL80_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108188.1"
                     /translation="MLFNETQLWFKFDPTNRFIKDFYKVWNSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
BASE COUNT         3000 a         2387 c         1833 g         3597 t
ORIGIN      
        1 aaattttgac cgaaattcat ttcatcactt tcctttcttt tccattgctc ggtccgccgc
       61 tgcacttgca tgcatcaagc acatcacagt aacaccaagc atactgccac caaacaccaa
      121 taaaatatct ttcacaattt ccatcacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatctttttg tgccggtctg atagtcgagt attatttacc
      241 atatcctgaa tgtacgttgt ctgattcaac cgatccaact ccttggccat cttccacgta
      301 ggggcgactt gtcgtccaag ccagttcaac gttcgttcaa tggtataagg cttcggttca
      361 gaagttaatt gaatttcctt ctgattccgt ccaatgaagc gcccccattg ttcattcaag
      421 ggccagttag ttcgcctttt gccttccacc ttatccgcaa atcgcaagta ccggttaata
      481 atggaaaatg tggtactttc aatactgcga ccttttaaca aatcttgaat tgcatgataa
      541 gctcgatcat ttttcagtcg gatttcaaaa cgatttttcg tttcggtctg gtctaaagga
      601 atccctaact tgatatattg ctcatagtct ttttcgtaca aacagaaata gacctcactc
      661 ttaagactgc caatatacaa agtattgccc ataccatctt tatcatcagg tttcaacagt
      721 tccccggaac gatagctttt gaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta aatcaggaat atctaacagg ccaactcgat cattgattgc caggtctaag
      841 cgtttcatca cgccaccggc ttttagacaa tcttcgaaaa agtcatacca gctacgcttt
      901 tgggcgagta aaaaagtttc aaactgccga caaccacgac cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcggc atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc actctctttt gaattggcat
     8221 ttggagtaat cactagttga ttgccgttca cacgataatc aatatttgcg atattctcga
     8281 caacttcatc aaactccgat aaattcaagc cgttcgtatc tgtcacagta gtcgattgac
     8341 ctaacacggt tttcactgtg gagttatgga agcttggttt cttttgataa tccgcaatgg
     8401 cttgattgat ttttgactca atggctgcga tatttactgc actaccatca gaacgtttaa
     8461 ttgagtgaag cgtagccatt aacttcttgc caaatcatct tctgggccac aatatgtgta
     8521 tccacatccg taccagcttc cttccataaa acggaaacta atttcgcttt ctccgacata
     8581 tcaggcaatg gatttttctc ataaccagga gtgaactcat tgtaaatcgg cacccctggc
     8641 tcaatacaga aaatatcttg cttcacacca ttgtaaactg catagtgcgg aacggtaact
     8701 tctgacatgg gacgaccatc gctaaatgta ccttcgacag catactggtg agccaaatcc
     8761 aattccacag ttaccgtttg tggatgagta atttcatcag caaaggcaat cgttgggcca
     8821 agcgatccac caattgtttg gcataataga ctcagtgttg cgagcaaacg ccaattctta
     8881 aatcttggct ttttcataaa ataaatctcc tttcgttttt gggtaataaa aaagacgtcc
     8941 aaattttaat tttgaacgtc tgatttagag tgatactatt tagttttctt tgtcttggat
     9001 aggttgtttc tctgcttata aattttgatt cttcgataat caaatccaat ctctgccatc
     9061 aattcctccc aatttttgtt cgttcttttc ataattgtat caactgatgg tagttttggg
     9121 gattgaacta gttttcgaaa ctttgtcatt gataaatcat cctgtagttt taatcggatt
     9181 atttcatttt ttagttctaa cagcagttcg aaatcagtaa atcgagatcc atcatttttt
     9241 tctttaaaaa gtggacgtat atctcctaac atctttttaa tggtactaag tgaaggaata
     9301 ttcttcccta ctgatttttg ttcgtacatt cgttgagagg ttatcttctc gcacttaata
     9361 aaggcttcta ctatttccaa taattcttgt tctgacattc tagcccattt tccataccca
     9421 gtattttctc gtcctatact aatcaataaa ttctcccatg atccatattt tttgttaatg
     9481 aaccacatac ttggtgcaga atttggattt tctgatagct ttttctggta ttgacggcta
     9541 gatgtaattc cgtgtttttc gatgaattca ttaattgtta aaatcagttt taactcactt
     9601 tgatttttcc aaatgatacg tccatcattt tgcttcacga taaaaactcc taatagttat
     9661 ttagatagaa gcctatcaca tcaacttttt agtagaaaag tacatgtttt gtcttttttg
     9721 ctaatagctg accctttgag aaacttgggt tcaaaagttg ctaaattcgg taaatgtttt
     9781 ggtatttttc gtattatatt tgtcgtcaag ctgactacta aattttgccc tattttgacg
     9841 acactgatag tcaatgtcgg acaatctagg acaattttta agggaatatg attgaaaaat
     9901 ggtccaaaac agccaaaaat ggcttaaaac tgtgatagag aaaaaagaaa aagcccttga
     9961 tatacaagga cttttcacaa atttcagatt tccaaggaag ttttccgtgt ttatcttctg
    10021 atttctttga tacgagcagc ttttccgtgt aatgcacgta aacaaaacca ctatattcta
    10081 ttatgtatct aataccctca aaacagctat aaatcaacaa taccgacata taataaacac
    10141 tatgttcaat ctgagatata tcaaagataa acgtattttt gccccctttt tgcccctcaa
    10201 atatcgcttt acaacgagaa cgtaagttcg tataatcgtt ttgaggtgat ttttatgatg
    10261 gaagacttta ttagaaaaaa tatcagtgat gaatatgcag acttttatga gcaaagcagc
    10321 aaaaaagata aattccagat ggatgtttca attttagcta tattagcttt ttccgaaaat
    10381 aaacaacctg taactgcaaa aaaagaaaca gtattctctg aaggcaaaat aaaaactcga
    10441 tatatattag aggtagaaac taattttaaa aacagatcgg agtaatggtt atgcttttta
    10501 atgagacaca attatggttt aaattcgatc ctacgaatag atttatcaaa gatttttata
    10561 aagtatggaa ttcagaagtt ttctttttag caatcgaaga tagcttatta atcaatctct
    10621 actattctaa taagaactat ttcaaaattc ctgctgcgaa aactagaatg aagaagggtg
    10681 tatacttttt gtttgatatc gtgactgacg tgccggacgc acgtagcgat catcggcgtt
    10741 atgactatat aaagtatact ttcgttgatc cagaaagata caaagattaa ataaaaaaat
    10801 aacaaacaaa ctcgctt
//
LOCUS       DARBEC010000094        10513 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.098, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000094 DARBEC010000000
VERSION     DARBEC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10513)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10513)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10513
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.098"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            34..657
                     /locus_tag="SCL80_002347"
     CDS             34..657
                     /locus_tag="SCL80_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108189.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1107..2156
                     /locus_tag="SCL80_002348"
     CDS             1107..2156
                     /locus_tag="SCL80_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1108190.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2153..3034
                     /locus_tag="SCL80_002349"
     CDS             2153..3034
                     /locus_tag="SCL80_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108191.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3037..3807
                     /locus_tag="SCL80_002350"
     CDS             3037..3807
                     /locus_tag="SCL80_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108192.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4086..4940
                     /gene="aroE"
                     /locus_tag="SCL80_002351"
     CDS             4086..4940
                     /gene="aroE"
                     /locus_tag="SCL80_002351"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEG1108193.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5118..6281
                     /locus_tag="SCL80_002352"
     CDS             5118..6281
                     /locus_tag="SCL80_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1108194.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6398..6808
                     /locus_tag="SCL80_002353"
     CDS             6398..6808
                     /locus_tag="SCL80_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEG1108195.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6982..9210
                     /gene="topB"
                     /locus_tag="SCL80_002354"
     CDS             6982..9210
                     /gene="topB"
                     /locus_tag="SCL80_002354"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEG1108196.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9381..9665
                     /locus_tag="SCL80_002355"
     CDS             9381..9665
                     /locus_tag="SCL80_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108197.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9825..10061
                     /locus_tag="SCL80_002356"
     CDS             9825..10061
                     /locus_tag="SCL80_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1108198.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10058..10456
                     /locus_tag="SCL80_002357"
     CDS             10058..10456
                     /locus_tag="SCL80_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEG1108199.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
BASE COUNT         3666 a         1767 c         2228 g         2852 t
ORIGIN      
        1 taataaaaaa taggagaaaa agtagaaaag aaggtgtttg ttatttggga agtagttaaa
       61 tcgagagtac aagaaatgaa tgaacatttc tcagtgtcat tcgcagaaaa tccaagtaag
      121 tattttcgac gtcttgtttt tctgctcatc ttatcattgc tgttaacagt tgcaatgttt
      181 ttattgccgg aaaaaatccg agaagagcaa gtaaatagtc tcgtttattc gtccatacca
      241 accagcaaat tgaaaccttt tgattacagc aaagctgatc agaaaatcaa acaaagccgg
      301 gcgatatcgg tgatgttcag caaaccgaac gggaaaacct atcaggacat gcttgatgtt
      361 tttaatgatc ctgagcttat ggaagaattg aatcgaccga tcttttatta tccgatcgtt
      421 tatgatgtac atgaactgga acaaaaatat aacatacgaa cggatgaagt gacatttatt
      481 ttctttgatg gaggaaaaga agcaaatcgg ataactgcgg gtaagggagg aataactgat
      541 tttgataagg agttgatccc tgaactgaat cgacttccgt tagccaatat caaacaatta
      601 gaagaagaat tggatagaac gggagtaact tcccaaaatt ccgagattgc taattgagac
      661 attggcgcga tgaaacggtg ggtctatata gcgctatttt taattatcag aacaagtgat
      721 gtttgatctt caaaataaca aataaaaaat taaaaaatat agttgacgaa attcaaaaat
      781 tcgattagac ttaaatcaat tcttaaggaa agggtgacct gtaatgaact tatcaaaacg
      841 tatttcagca gaaaacaaaa aaaattggcg cagctggcgt agataagttg tattaatggt
      901 gagactttcc catagataca actttttaga gtgcatttct aaagcttgta tctatgctag
      961 acatgaacat tgactgccgc atagattaga atctatgcgg ttttttgtgt aaagacaatg
     1021 accgctttgg ccattgtctt tttttgtaga ctggatactt gcctgattat aataaataaa
     1081 aagattgact ttgcaggagg aaaaaaatga aaaacaaacg attaataaca gttgtagtaa
     1141 ttattttagt ttatttagca ggtgcattta tttatggaaa agcctcaggc gaaaaagaaa
     1201 gtacttcgac gaaccagaca aaaaaaacaa cagttgttgg ggtattgcaa tacgtgagcc
     1261 atccggcatt agatgagatt tatcgaggaa tcaaagatgg tctgaaagaa gaaggattaa
     1321 aagaaggaga aaatcttgag atcaaatttc aaaatggaca agccgatcag agcaagctag
     1381 ccacaatgag tcaacagcta gtacaatctg atccagatgc cttgattgga atcgcaacac
     1441 ctgccgctca atcattagca aacacgactt catcgatccc agttgtgtta ggtgctgtaa
     1501 cggatcctgt gggggctgga ttagtcgctt ctttagagaa acctggtggt aatgtaacag
     1561 gtgtttctga tcagccgcct gttacttcac aaatcaagct tgcttctgat ttgttgccaa
     1621 atgcaaaaaa agtcggaatc ttgtatgctt cttcagagga taattcaaaa taccaagtag
     1681 cacaagcaga agaagctgca aaaaaagaag ggttagaatc agtaaaatat gcggtgcctt
     1741 ccactaatga gattgcacaa acggttcaag tgatgagccg acaagtcgat tttatctatg
     1801 ttccattaga taataccatt gccaatgcga tgcaaacagt ggtaaaagaa gcaaacaaag
     1861 caaatatccc cgtcattcca tctgtggata caatggtgga acaaggtgga ctagcaacaa
     1921 tcgggatcaa tcaatatcaa ttagggcttc aaagcggtaa gatggcggca gaacttgcga
     1981 gtggaaaaga aaaaccggaa acgacgcctg tttatacatt tgatcaaggg gatacagtga
     2041 tcaatcaatc tcaagctgat cgcttaggaa tcattgttcc ccaatcgatg aaggaaaaag
     2101 ccaaaatcat tacagatgac tcgcagcaag aaacatctaa ggaggatgac aaatgatcgt
     2161 atcagctatt tcacaaggca tgctttgggc aatattagga ttagggattt tcatgactta
     2221 tcggatctta gactttcctg atatgacgac agaagggtcc ttcccattag gtgctgctgt
     2281 ctgtgtgaca gctattactc atggggtagc gccgattgta gctacattat taggcgtagg
     2341 tgctggcatg atagcaggac tcgttacggg gttactttat acaaaaggaa agataccggt
     2401 tattttagca gggattttag tgatgtcagg attgaactca gttattttgt ttgtgatgcg
     2461 aacaccaaat ctttctttat tgaatcatcc gattctacaa gacagcttcc atcgctttgg
     2521 gttacctgat tactttgaca cagtccttct tggcttcatc tctttgggca tcttgattgc
     2581 tgtactgcta ttcttcttca atacagattt gggtcaaggc tatattgcga caggagacaa
     2641 cgaaacgatg gctcggtcgt tagggataaa aaccgatcgt atgaaaattt taggtttaac
     2701 tttgtcgaac ggtgtcattg cattatccgg tgcgctgatt gcccagaacg acggatatgc
     2761 agatgtaaat aaagggattg gcgtaatcgt catcggtctt gcttccatca tcatcggtga
     2821 agtgatcttc caagaattga cgctagcaga aagattgatt gctattgttg tcggcagtat
     2881 catttatcag ctattgattt tagtagtaat caaactaggg tttgacacga cttatctgaa
     2941 gttgttctcc gctatcatct tagctatttg tctgatgatc ccgcaattga agaaagcact
     3001 gaacttaaaa acgggttttg agaaggaggc atagtaatga ctgatatatt gacaatcaaa
     3061 aatggcagaa aagtcgtaaa caatggaatg aatgaagaga aagtactatt ggatgaaatt
     3121 aaccttacaa tcagagaagg ggacttcatc actgtccttg gcgggaatgg tgctggaaaa
     3181 agcacactgt tcaatacgat cgctggtacg cttcaactca gtcaaggatc gatctctttt
     3241 aaagatcagc tgatcacaaa agattcagaa gaaaaaagag cacgattcct ttctcgagtt
     3301 ttccaagacc ccaagatggg aacggctcct cggatgacag tagcagaaaa tttgctactt
     3361 gcacaaaaaa gaggtcaaaa acggaaattc cgtttacgta acttgaaaaa tcaaaaagaa
     3421 gattttttca gactatgtca agaagtcggt aatggcctag agcaacactt ggatactcca
     3481 gcgggtgaat tatctggtgg tcaacgccaa gcattaagct tgctgatggc gacgatcaca
     3541 gctcctgagt tactattatt agatgaacat actgcagctt tagatcctaa aacagctaaa
     3601 gcattaatgc gattaacaaa tcagcggatc aatgaacaaa aattgacttg tctcatgatc
     3661 acgcatcgaa tggaagacgc cttgaaatac ggaaatcgtc tgatcgtact ccaaaaaggg
     3721 aaaatcgttc aagatatttc tgaaaaagaa aagcaacatt tgacaatggg cgaccttttg
     3781 cagttctttg aagaagtagt ggattgattt cgacagaaaa attcctttca gtagaaggac
     3841 ttgaaaactc attgacatct tcctttgatt catcctatac ttaaaacaat aaacaattaa
     3901 acatatttgg gtgttttgta ggatcacgta gtcaatgatt ttaacaatta aatagaatat
     3961 tctttgctct gacaagtaaa cgatgagaaa agaatttgct actttgccac ctagacgttt
     4021 cgtacttttt cgtatcaaac gctaagtggc tttttttatt gaagtaagaa caaaggagga
     4081 ataagatgat ttctggacaa accaaattag ctgggctctt tgctgatccg gcttctcaca
     4141 gtttatcccc attgatgcac aatactgctt ttgaagcaaa caacatcgat gcggtgtatt
     4201 tagctttcca agtaaatcca atgaatttga agcaagcagt cgaaagtatt cgaacttttg
     4261 atatgttagg tgtaaatctt tcgatgccga ataaaacagc ggttattcct tatctggatg
     4321 aggtaagcca ggaagcgcaa ttgatcggag cagtgaatac gatcgtgcac gaaaagggtc
     4381 gtttgattgg ctataatacc gatggtgcgg gcttcatgca atccgtgcaa gaagctggga
     4441 tttcgatcaa aaagaaaaaa atgactctac tgggagctgg aggtgcagca aaggcagtca
     4501 ctgttcaagc tgcgttagac ggcgctgagg aaatcgtagt ttataaacga aacaaccaaa
     4561 cattcgctca agtaaaagaa acattcgaac aactcagcta tcgaacttct tgtaagataa
     4621 aagttcgtga ctttgccgat acgcatcaat taagaaaaga cttgaaagaa agctgtcttc
     4681 tagtcaatgc aacagatatc gggatgggaa agaaaatcaa tcagtcaccg ctcttagata
     4741 taagcatgat gcatgagaat ctagcggttt ttgatttgat ttatgcacct agagaaacac
     4801 gtttattaaa agaagcaaaa aaagtaggag caaaaggata caatggactt ggcatgttga
     4861 tctaccaagg agcaatcgcg tttgaattat ggacaaagga aaagatgccg atccatgtga
     4921 ttgaaaagct gtttgattaa aaaaggataa tagataaaga tcttttattc tcgcataaac
     4981 ggtctcaaga aaagtaatcc tttatgtatc gaagcattta tttcgttatt ttttattaaa
     5041 ttatcaaaaa atgataggct tttgtcgatt ggttatcgta tcctttattt atcagtaaga
     5101 aacgaggagg aaccacaatg agtcggatgg atcgctataa ggacatccat aagaaggcga
     5161 aacctctgaa gcgggatacg gggagtattt ttgctagaag agaacgaaaa cgaacgcgta
     5221 tggaaaatga ggaagatgct acccgtattt atgaaagaga gcagcattca tttgaacatt
     5281 tgaaccaata cgagcaacag atatacagag atgcaccaag agcagaagaa caacaaaaaa
     5341 ataaaaaatt tttcaatcgg caaaagaaga acaaaccgtt aaaacctcga agaaagcgat
     5401 cgtggaaaaa aatcattcta tcgattttgc ttgttttgat cctttattca ggtatctcat
     5461 tttttattgg taaatatgta gctgaacatg acacaagctt agaggcagcg acttcagaaa
     5521 cctttcatgg gcaaaaagcg acgaatggtg cgcataatat tttgatcctt ggaagtgata
     5581 cacgcggaga agatgccggg cgagccgata cgatcatggt cttacagttg gatggtcctt
     5641 cccataagcc gaaactgatt tcttttatgc gtgacagctt cgttacgatc ccaggggtag
     5701 gacaaaataa gatcaattca gcttatgcat acggaggagc cgatctagtg cgtcaaactt
     5761 tagtagaaaa ttttggaatc gattgtcaat actatgcgaa agtcgatttc aaatcgtttg
     5821 aaaaagtgat cgatgcctta ttcatgaacg gagtgaagat cgatgcagaa aaagatctga
     5881 atctggatgg tgtagatatt aaaaaagggg tccagaagat ggatggacat gttcttttgc
     5941 agtacgcacg tttccgaatg gatgaacaag gagactttgg ccgtgtcaga cgccaacagc
     6001 aagtaatgaa cgctattttc agccaattga agaatccatt gaatctgatc cgtgcacctt
     6061 atgcagctgg taaagcttta ggatatacat ccacagatgt cactgcttgg ttcttgacga
     6121 aaaacctctt ttcgattgca cgaggagctg gtggcattga tcgaataagt gtgccagtag
     6181 acggttcgtg gaattatggg caaagtgatt atgctggcag tattcttgaa attgataatc
     6241 aaatgaatca atctgctatt acagattttt tgaataaatg atcataaaaa agatcaagaa
     6301 gaagctgcgc aaggcttttg tcatgaatca atcaaacgat tcactcgtaa aaaacgcttt
     6361 cttatggtag atttaaccaa gagaggagct gtttacattg gatatctatc tgaacttcaa
     6421 aaatgagtcg cgtgaagcca tcgcttttta tgaaaaagtt tttcagactg aagcatcagg
     6481 tatcaccaca tacggtgaaa tgcctggaat agaacaaatg aacttgccag aagaagcaaa
     6541 aaatctaatc acgaatgcac ggctgaacat ttatgataca ttagttatgt ttgcagatac
     6601 gccagaagga attggtcctg aattagttgt gggaaataat gtttctttag tcatccagac
     6661 aagaaatgaa gaagaaacga agcgcctatt tgaaagctta gctgagggtg gtaagattga
     6721 gatgccactg gatgctgttc cgtgggctca gttgtatggg atggtaactg atcgatttgg
     6781 catcaactgg cagattaacc aagagtgaca gctaaagatg aatattttct ggaaacatca
     6841 agcagcaaac ggatcatcgt ttgtttgctt gatgtttttg tgtctatttc gactatacgg
     6901 tatctttttt ttattgccag atgcgaaaat atgttccata atagagaagt ccgcaaaaaa
     6961 ttaagtaacg gaggtcagaa gatgaagacg gtgatactag ctgaaaaacc ttcacaagca
     7021 aaagcctatg ccgaaacctt ccaacagcaa aagcggcatg aagggtatta tgaaatcagc
     7081 gatccattgt ttgctggtga agtaaccatc acttatggtt ttggacattt ggtagatatg
     7141 gttgctccag gagcttatga agagcgttgg gcaaaatggt ctttagaaaa tttgcctatt
     7201 ttcccagaaa ccttccgtta cgaagttcca atggacaaac gtgcacaatt ttcgatcgta
     7261 aaacgagaat tgcagtcagc cgataccatc attattgcaa cagatggtga tcgagaaggt
     7321 gaagccatcg cgtggtcgat tattcgacaa gcaaacgcat tttctaagga aaagaactat
     7381 ttacgtttat ggatcaattc tcttgaaaaa gaagcaatct atgaaggttt taagaatctc
     7441 caacctggaa agaactattt tccaaaatat aaagaagcgc aagcaaggca gaacgctgat
     7501 tggctgattg gaatgaatgg aagtccatta tatagcttgc tacttcaacg aaaagggatt
     7561 gatggcagtt tttctcttgg tcgtgtacaa acaccgacac tctatatgat ttatcagctg
     7621 caagaagaaa tcaaacactt caaaaaagaa acgtactttg aaggagaagg tttaattacg
     7681 acaaacaaag ggcagttttc tggaaaaatc gtaccaaaaa aagcatttaa aacccaagaa
     7741 gagttaacga aggaaataga aaaacttgga gcgcatttag gtaagcaaaa cggacggatt
     7801 cttgaagtta cgaaaaagga aaagaggttg catagtccac gtttattttc gctatctagt
     7861 ttgcagacaa agatgaatca gctaatgaaa gcaagtgcca aagctacact tgatgctgct
     7921 caaggattgt acgaaagcaa gtttctaagc tatccaagaa cggattcctt ttacattaca
     7981 gaaaatgagc atcagtatct ggttaaacat ctgcaaaatt ataaaaaatt tttaggaata
     8041 gaagaagtac agacaactga gatcaaagca aagaaacgat atgtcgatgc gaaaaaagtt
     8101 caagaacatc atgcaattat tccgacaaag acgattccga ctcctgcacg ctttgcgaag
     8161 ctttccaaat tacagcaagc aatctatttg caagttttgc gcactactgt ggctatgttt
     8221 gctgcagatt atatctacga agagacgacc gtaatgacgg gcgtacagca attaaagcta
     8281 aaaagcatcg gaaagatccc attacagcaa ggttggcagg aaatcttacg catcaagtca
     8341 aaaaagaaag aagctcagac tcttcctcct atcgtagaaa atgaagaagc agaagtagaa
     8401 ctgaaaagta tggagaaaga aacacaacct ccaaaaccct ataccgaagg aacgttgatc
     8461 acagcaatga aaactgcggg aaaaacgctg gataatgatg aagcgcaaga aattctcaaa
     8521 gaagtagaag gaatagggac agaagctaca cgagccaata tcattgaaaa tcttaagcag
     8581 aagcattaca tcgaagtcaa taaaaacgaa ataactgtta cgccaaaagg aatcactctt
     8641 tgtaaggcag tagccaatga accattactg acgagcgcag aaatgactgc gcgctgggaa
     8701 gggtacttga gaaaaattgg taaacaagag gggacaccat ctgttttttt agagaatatc
     8761 aaaaaattca tcctacattt attggcagaa gttcctaatc aaatcgacaa ggtagacttt
     8821 tcagatgaga ttagcgggac acgtcagata aaagcgatgg agaagaaaaa cgatcaatta
     8881 ggaagatgtc caaaatgtaa aaagggaata gtcatgcttt atcctaaaat cgctacatgc
     8941 ttgaatccgg aatgcgactt caaattgtgg ccaacagtag ctaaaaagaa gctgactaaa
     9001 acgaatctca gagatttgct ttcaaaagga aagacaagta aagttgtcaa aggttttact
     9061 ggtaaaaaag ggaaattcga tgcagtttta gtattaaaag aagatatgac aatcggtttt
     9121 tcttttccat ggatcgaaaa cagtggaaca aaagcagttg atggaaacaa agaaacatca
     9181 agaaaaaata atgaaaacga agtagagtaa atgaaaacaa tgaaatagcg gaatatttat
     9241 tccgaaaagt gagaatataa ataccaataa aactaaaaaa tatcagtgaa tcaagcaaaa
     9301 acgttggggt aggctgattt tatatagata gcaaggagtt tttttatttg aaaaaagtgc
     9361 attcattttt aaattatctt atgggagcaa tcacgattgt ggctgtgtac caagcatttc
     9421 taaagtatcc tgattacggg acgaagttgt tgtcattgac gcacaatcaa acagcatatt
     9481 caccaacgtt gattttatcg actttgactg caggagctaa aagcgctact tatttttttc
     9541 ataaaacgac aagcatgcta aagcgaaaga aacagcgaaa acaattgttg gtggtggtag
     9601 cggctatgat cattatttat tttctaccag ttattacaca agtatttcat caatattttg
     9661 gttaagaata cgagagtttg tgacaaaagt catgtcacaa acttttttct ttgtcatttt
     9721 ttcgtaagaa aaagcaaaat aaaagtatat acattgatta aaagtatata cttggatata
     9781 atgtacttac tatagaatga gaatggtata gagggtggcg aaatatgaaa aaaatgaaaa
     9841 agtataaaat tagaaaatct ggcaatagcg atgtaacaac tatcccacca gaagtaaaag
     9901 aattcatggg tgtgcagaca ggtgacgcaa tcagttatgt ttttcaatct gatggttctg
     9961 tacgaatgat caaagcacaa gaagaacctg atattgattc attagttgat tcgatcatga
    10021 atcaatatga agacgcgtta aaagatttgg ttgatttatg aggtatcttt cgactaaaga
    10081 aattatcaaa atcaacgcga aagttatttt acgtcactcg ccaggcgaaa tgatcggtgt
    10141 aaaagatgca aatgcattag atatggccgt caaacagcct tcacaagcag tattcaatca
    10201 agaactctat cctgaaatct atgaaaaagc ggcgatttta gcaattaatt tagctaaaaa
    10261 acatcctttt cataatggca ataaacgaac tgctcttgtt gccatgttgt tgtttcttca
    10321 attgaataat tgtccagttg ctttttctcg tcaagaagct gttgatttta tcataatgat
    10381 taccactagt tcattggatt tcgattcact aaagtccaag ataaccagtt atttacgaca
    10441 aaccgcaata aaatgaacga attaaaaact ttatttctca attagatcag catgtcatta
    10501 aggaatatgc tga
//
LOCUS       DARBEC010000095        10232 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.099, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000095 DARBEC010000000
VERSION     DARBEC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10232)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10232)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10232
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.099"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            314..1093
                     /locus_tag="SCL80_002358"
     CDS             314..1093
                     /locus_tag="SCL80_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108200.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            1139..2083
                     /locus_tag="SCL80_002359"
     CDS             1139..2083
                     /locus_tag="SCL80_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEG1108201.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            2099..2878
                     /locus_tag="SCL80_002360"
     CDS             2099..2878
                     /locus_tag="SCL80_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEG1108202.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2890..3588
                     /locus_tag="SCL80_002361"
     CDS             2890..3588
                     /locus_tag="SCL80_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEG1108203.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3616..4380
                     /locus_tag="SCL80_002362"
     CDS             3616..4380
                     /locus_tag="SCL80_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEG1108204.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4424..5875
                     /locus_tag="SCL80_002363"
     CDS             4424..5875
                     /locus_tag="SCL80_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEG1108205.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5907..7043
                     /locus_tag="SCL80_002364"
     CDS             5907..7043
                     /locus_tag="SCL80_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1108206.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDVVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            7043..8149
                     /locus_tag="SCL80_002365"
     CDS             7043..8149
                     /locus_tag="SCL80_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEG1108207.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            8155..9177
                     /locus_tag="SCL80_002366"
     CDS             8155..9177
                     /locus_tag="SCL80_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEG1108208.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9199..>10232
                     /locus_tag="SCL80_002367"
     CDS             9199..>10232
                     /locus_tag="SCL80_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEG1108209.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTR"
BASE COUNT         3738 a         1442 c         2100 g         2952 t
ORIGIN      
        1 aactattatt aattcaatgt taattttttt gttattctat tttttgtgta acttttaaaa
       61 acacctatga aaattatgga aataaaaatg aaatcgatta atttttatct tattaatgtt
      121 ttatttactt ttaaatcgta aattgcccta gtcaagaaaa cagtttgtgg tatgattata
      181 tagggaaaac ttgtttacaa atatttctgt gaatatatta gtttaattat gtttttttaa
      241 ataaaaagct gtcattttta tgtaagtgaa ttatatctta atttatctat catcaatact
      301 taggagtgaa tctatggaaa agccattcgt caagtcattg actgctgtgg cggtaggtgc
      361 aggtgctatt gcgatctgtt tgtttggata ccatatcaat aatcagcggc aacatcagca
      421 acggatcaat tatgcagaat ctgccatcac caatcaaaaa gacaccttga cttctttatc
      481 aaaagaagta gacaagctgt atagtacaaa agaaaaaatt tttctaaacc ctgaaataac
      541 agaagaaaca atcacaagtt tgtcaaataa gctatcctct gtcaaacttt cagcagatga
      601 ttacgacatc aaagaatcag aattgcctaa agaagctgtt gcaatccaag atgagaagaa
      661 agcaatcgtg acacagttag atagtgcaga atcaaaactg aagatccaga cagctgtcaa
      721 cgatttattt actaaaaatg tatcaaactg gcagcaagct gtagacgatg tcatcatcaa
      781 agaaaaggtg acaagtgcag atgtcgctcg tgtacgtgaa aatatgagct tttttaaaga
      841 tagtccttgg aaaacggttg ttatgcaata tctaggattc gctgatattc agatcgcgca
      901 agtcacgcaa ctagatcagt tattcgatac gatgttaaag gatggtcaag tgactgccac
      961 cgctacttac gatcaatatt tgacggtgct cagtcaaata gagcagatcc ggaatgaaaa
     1021 aataagcgca gcatacgcaa caaaagcaca aactgttgct gagcaaatgg gctatagcaa
     1081 tataagctat tgacagttaa aacgttgatt caagcaaggt tagcaaacgg aggaaactat
     1141 ggcaagacga agaagaaaat caagagggaa aaaaatattt ctgattattt tagcagttct
     1201 tatcgtatta gtattagcaa ttgtaggagt aggagcaaaa ttatatctgg atctctcaca
     1261 ttctattgag aaaacttacg aatcagtgga acgaacccaa ggagaacgaa cgaaaccagt
     1321 tgatctggac aaacaagaat ctttctctgt tctattgatg gggattgata ctggagattt
     1381 aggtcgggtc gatcaaggaa gatcagatac gatgatggtt gccacagtca gtcctgaaga
     1441 caaacagaca acgatcgtca gcattcctcg tgatacgtat gtcgacatcg taggtcgtga
     1501 cacaaaagac aagatcaacc acgcttacgc attcggcgga gctgccatgg cgatggatac
     1561 cgtacaaaaa tatgtagaca tcccaatcga tcactatgtc tctatcaata tgaaagggct
     1621 aaaggaactc gttgatgcag ttggtggtat cgaagtcaat aatgacctca ctttctcaca
     1681 agatggctat gactttccaa tcggtaaaat tacattgaac ggggatcagg cattatctta
     1741 ttctcgtatg cgttatgaag acccaaatgg tgattacggt agacaagggc gccaaagaaa
     1801 gatcgtagaa ggcgtagcga aaaaagtatt gagtctaaac ggtgtcagca actatcaatc
     1861 cgttttgaat gctctagagt cgaatatgaa aacagacatg aactttgatg aaatgaaaaa
     1921 aattgcttta gattatcggg aggcatttgg tacgattaag caagatcagc tgcaaggaga
     1981 aggcttcatg caagataata tctcctatca aaaagtcagt gatcaagaac ttgatcgtgt
     2041 gaaaaaagaa ttaaaagatc aaatgaatct agagaataaa taggaaagtg gagaaaaaat
     2101 ggaagagaca atcagcttga aagaaatttt tgaaacatta cgcaaacact tggtaataat
     2161 catcatctca atgtttgctg gcttggccat ctcaggaatc gtaacatttt tcatcttgac
     2221 accaaaatac agttcacaag cacaattgat cgtgacactg ccacaatcag acactgcaaa
     2281 tgtcaatgat gtcaatacga acctgcaaat gatcaatacg tataaagata tgatcgtcag
     2341 tgatttagtg ctcaatgaag tcaaagatcg tttagaaaca gaagacaatg tgaaaatgac
     2401 tgctgaacaa atcaaagatg ccatctcagt taatcaggca gaaaattcac aaatgttctc
     2461 gattcaggca ataagtacga atgcagtggc tgctcaacag atcgctaaca caacttcaca
     2521 agttttccaa gagaacgcaa aagatgtgat gaatgttgat aaaatctcga tcatttcagg
     2581 tgcagttgcc aatacgacac ctgtttcgcc aaataataaa ttgaatttaa tgactggctt
     2641 aatgttagga atgatggtag gtgtaggtct ggcattctta ttggaattac tcgatcggac
     2701 agtgaaagat gaaaaatatg tgacagatac attaggattc ccaatcttag gaacagttcc
     2761 agaaatgtca gcgaaagaat tgaatgctgg tatccgtaaa aaaggaccag ctcagacaca
     2821 accaaaagct acaaacgata aaacagcgcc atcacgcaga agccgctcaa gagtctagga
     2881 ggaaagaaaa tggcacgaac acagaaacaa aaagcagccc aaactcatgc agtcagtttg
     2941 gtcacactca ccaatccctc ttcaccaatc gctgaacaat atcgtacgat tcggacgaat
     3001 atccagtttg cctccgcagc tggtcagcaa atcaaaacga ttgttgtgac ttcatcagga
     3061 cctggagaag ggaaatcgac cactgcagca aacattgccg ttgtttttgc caaatctggt
     3121 caacgggtgc tgctagtcga tgcagactta cgtaagcccg tcatctataa aacgttcaag
     3181 ttgaacaacg caagcggatt aagtactgtt cttagttcat caggaagtgt agcagatgag
     3241 atccaacgaa caccagtcga caatttgtcg attctgccaa gtggacctaa accaccaaat
     3301 ccatcagaac tactaagttc gccaagaatg gatcagattt tagcagaagc aagacaacta
     3361 tttgatgtag tgatcttcga catgccgcca gtcgttgccg taacagatgc acagatcatg
     3421 tcttcaaaaa cggatggcac cttattggtc gtacgggaaa atacgtcacg aaaagaatca
     3481 ttgactaaag caaaagaact gttagatatg gtacaagcac gagtcttagg tgtcgtatac
     3541 aacggagcag aacacagcaa agatgctggt tattactacg gaaattaagg aataagaaga
     3601 gaggagacaa gagaaatgat cgatctacat tgccatatcc taccaggaat cgatgacggg
     3661 gcagaaaact tagaagcaag tattgcaatg gctgaaaaag cgattcagca aggcattacg
     3721 catattttat gcacaccaca ccacaataat ggaaaataca gcaatgaaaa atcacaagtc
     3781 atttctctag tcgcttcgtt acaagccgaa ttagagaaaa gacagttgcc tttgacgtta
     3841 ttggaaggac aagaagtacg tattaccgga acgctcatcg aagacatcca ccgagatgaa
     3901 attttattta cagatttaga tgatacatat cttctgatcg aatttccgac actggaagtg
     3961 ccgttatatg cagaacggct tttttttgag ttgttagaac taggaaagat accagtgatc
     4021 gtccatccag aacgcaacgc gcattttcga gaagacccaa atcatttgct tccgttccta
     4081 gatatgggat gtttggccca acttactgcg ccaagttatg ttggtgtatt tggcaaagat
     4141 atccagaaaa cagccaaaac aatggtcaaa cataatctcg tgcagatggc tgcttctgat
     4201 gcacacgggg tctctaagcg caccttttac ttaaaagagt gctatgagca gattgccaag
     4261 gagtttggta aggaaaaagt agtacagatg aaacaagtcg caaaggatct gatcaacggg
     4321 gatgagatca attatccaat ctatgaagag gtaaaaaaga agaagtttgg tttattttag
     4381 ttctttattc gtagataaaa aggaaatgag gagagagtga gagatgtata gaaaaaactc
     4441 aagtggttgg tctaaacata ttgattttat tttattagac ttattttgtg tccagcttgc
     4501 tttttatata tcatatgtaa tgcgacaggg tgactggaat ccgtatgtga ttcctattta
     4561 tcgccacatg gcaattttta tagaacttga gaatcttacg attatatttt tatttgaggc
     4621 atataaaaat gttttaaaaa gaggatatta taaagaggct gtagcttcta taaagcaatc
     4681 ttttatgctg ttacttgtgt gcagtttata ccttattaca atgcaggatg gaaatgatta
     4741 ttccagagtt tctttattta taatgggggt ggtgtatgga ttattaacat atataattcg
     4801 tatactttgg aaagggttgt tgcatcaccg catgaaaaac ggtgaagatg tatcgttagt
     4861 tattgtaaca tctcaaaaga ttgcaaaaaa tgtagttcgt aatcttaaag agaagaatta
     4921 tggcatgtat aaacttagtg gtgtgattgt agtagacaga gatatgtatg gaactaccat
     4981 cggaggtgtc ccagtagttg caaatgctga taatgcagcg gaatatcttt tacagaattg
     5041 ggtagatgaa gtattcatca atgttgacga aagtgttcca tatcccaaag aattaatcgc
     5101 cagatgttcg gaaatgggac ttgctgtaca tatgaatctt acaaaagtgt ctgatagtac
     5161 acgtggaaaa caatttattg gccgagttgg tgattatact gttattacat ctagtattaa
     5221 tgttatgacg atgaggcaag catttataaa gagaacgatt gacattcttg ctggaattat
     5281 tggatgtatt gcaacaggtg ttatttttat cttcgtagca ccagttattt acatcaaatc
     5341 accagggccg attttctttg cacaagaacg tattggtcaa aatggtaaga catttaagat
     5401 gtataagttc cgtagtatgt atatggatgc agaagaacgc aaagcagagt tgatgaaaga
     5461 aaataagatg agtaataatc tcatgtttaa gatggatttt gacccaagaa tcattggcaa
     5521 taaaatactt cctgatggaa caaagaagac aggaattgga caatttattc gatctacatc
     5581 attggatgag tttccacaat tttataatgt cctaatggga aaaatgagcc tggtggggtt
     5641 tagaccttgc ttgaagtcag aatatgagaa gtatgatttt catcatcgtg cgagaattgc
     5701 gatgaaacct gggattactg gaatgtggca ggtgagtggt agaagtgata tcacagactt
     5761 cgaagaggtt gtaaaacttg atgcagaata tatcaggaat tggagtatgg gattagattt
     5821 tagaattttg tttaggacag tcaaaactgt gcttcataga aacggttcca tgtaagataa
     5881 agatactata gaaggagtaa aacgtgatgg caagaaaaaa attaaaaatc tgtatgctag
     5941 gtcataaaag aattccatca agagaaggtg gaattgagat agtggtagaa gaattggcaa
     6001 cgcgaatggt tgcacttggg catgaagtga cttgctataa tcgaagtggt catcatgtta
     6061 gcggaaagga atttgaccag cataaaaata aagaatataa aggtgtaaaa ttaaagacta
     6121 tttttacatt aaatataaaa ggaattgcag caatgagttc ttcagtattt ggtggaatta
     6181 gagcagcatt cggaaaatat gatgttgttc attttcatgc agaaggacca tgtgctatgt
     6241 tgtggttgcc aaaacttttt gggaaaagat gtgttgcaac aattcatggt ctagatcatc
     6301 aaagagaaaa atggaacaag cttgctagta catacatcat gttaggggaa aagtgtgctg
     6361 taaaatttgc agatgaaata attgtactta gtgaaagcgt ccaaaattat tttgaagata
     6421 tatatggaag aaaaacaagg tttataccaa atggcgttaa aaaaattgaa ataaaatcag
     6481 caggattgat tacagaaaag tatggattaa caaaagattc ttatattttg tttttggggc
     6541 gattggttcc cgaaaaagga attcggtatc taattgaggc atttaaagat gttcagacag
     6601 ataaaaaact tataatagcc ggaggaagct ctgatacaga tgaatttgca aatgaactaa
     6661 aggaattagc aaaaggtgat gagcgaatta tatttactgg atttgtacaa ggacaggaat
     6721 tagaagaatt atatagtaat gcgtatatct atacacttcc aagtgattta gaaggaatgc
     6781 cattaagttt gttagaagca atgagttatg ggaactgttg tatagtttca aatatctcag
     6841 agtgtacgga agtagtagaa gacaaggcta tgatttttaa gaaatcggat gtaagtgatt
     6901 taaagataag attagaagag gcatgcaatc aatcggaaat ggttaaagtt ttgaagaatc
     6961 aagctactga gtttatttgt agtaaatata attgggataa aatcgtacaa gaaacattaa
     7021 atttgtatag gggaaatata taatgattcg aatattacag tgtgtgaatg atatgcatcg
     7081 tgcagggttg gagacgatgc taatgaacta ttataggaat atagatcgaa ctaaaattca
     7141 gtttgatttt ttgacacatc gtccatataa aagtgattat gatgaggaaa taataagtct
     7201 tggaggaaag gtctattatg caccgagact ttatccacaa aattatccta aatattttaa
     7261 atggatggaa caatttttta aggatcatcc agaatatgag attgttcatt ctcatattga
     7321 tgcgatgagt tatttacctt taaaagctgc aaaaaaagct gggattccga tacggatagc
     7381 acatagccat aatacatcga tagatagaga ttttaaatat cttttaaagc aatattatcg
     7441 atgtaagtta acgtctgttt tgacacatga attttcatgt ggaaaagtag caggtgattt
     7501 tttgtttcga aatgataaat tcactttgat tccaaatgca gtggatgcta aaaaatttta
     7561 ttatgatgaa agtattagaa atgatgtacg aatgaaatta ggaatttcaa gtcagacgtt
     7621 tgtaatggga catattggaa gaatttcata ttctaaaaat catcgttttt tgattgaaat
     7681 ttttaaagaa tttcacgcta ttaagaataa ttccgtatta ttaattattg gaactggtga
     7741 tatggaagaa gaaataaaaa attatgcaaa gaaaagtggc atagatgatg atattaaatt
     7801 tttaggaaat agaaatgata tagaaaaatt ttatcaagca tttgacgtat taatgttgcc
     7861 atcattattt gaaggggttc cgttagtggg tattgaagct caatttgcag atttaccgtg
     7921 tttcttttcg gagaaagttc ctacagaagt tgctttcagt gataagacca atttcataag
     7981 tttaaatcaa tcggcaaaag aatgggcgag agagattgca aatgtagata tctctcatcg
     8041 agattcagaa cgttcgattt taataaaggc agattataat attcaaacag catacaaaat
     8101 cttagaatca aaatattatg agttatttga attaatacaa agaggttaaa gaatatgaca
     8161 cagaaacatt caagtataaa aagaattata catcgcatat ccgaagaatt gaatgcaaca
     8221 agacgaattg caaagataac atcttggaag gaagcatttg ccacgttaca agctaaaatt
     8281 gatattcaag tcatgaatcg taatggatat aatgaaagta aaaaacagaa ggaacattta
     8341 ttaaaaaaac atgaggtaat gctgaagtat tatgaaaaga ctttttcaga ttttttgaaa
     8401 acatatgatt atagtcaaaa tcaaataatt aatcaatcaa aatcaccata ttctaactgc
     8461 atttgggttt gttggtggca gggattggat caatcgccgg aaattgtaaa agcttgtgta
     8521 aaatcaataa aacgaaatgc agggaaacat actgttataa ttttaacaga agataattat
     8581 agacaatatg ttgatatacc aaaatgggtg gaagaaaaga aaaacaaagg aatcattacg
     8641 agaacaaatt attctgattt attaagatta tctttgctag caaaacatgg aggtttgtgg
     8701 ttagacgcca cattttattg tacaaaacca ataccagatg agtattttga tatcgatatt
     8761 tggtctatta ggaggcctga ttatgctcac gcatccgtag catcaggata ttttgcgggt
     8821 tattcattag catgtaatca agagtctaga tggatgtttg ctataattag agactttttt
     8881 ttaaattatt ggaaaaataa tgatatgatg gtggattatt tgatgataga ttatatgatt
     8941 gttttagcac agagaataaa tcatgaaata gcacagacat ttgataaaat acctagtaac
     9001 aatccattat gtgacgaatt gattaaaaat ttaaataata tatatgatga aaaagtatgg
     9061 aacttattaa agaaagatac tttactgttt aagttgacat ggaaacaatc ttttaaaaag
     9121 caaaaagatg ataaagttac tttttatggg atgttggtag aaaacaaatt attataaata
     9181 ttagaggtat aaaaagttat gaaacacaag gtaagtgtga tagtaccaat ttacaatgtt
     9241 gaaaaatatt taagaaaatg tgtggatagt ttattgagtc agacattaaa agagatagaa
     9301 atcgtattag ttgatgatgg ttcgccagat gcttcggggg aaatagcaga tgagtatcaa
     9361 aaaaaatatt ctaatgtaaa aacaatacat agagaaaatg gggggttagg accggcaaga
     9421 aatacaggaa ttgagaatgc gacaggagaa tatgtggctt ttcttgattc agatgattgg
     9481 gtagaaagtg atatgtatga aaaattatac ttagttgcgt ccaaagaaaa tgctgatata
     9541 gtagttagtg gacattgtga ttttgcgaat gacaaagcta tggtgaaaaa gcaacatcct
     9601 ttgagtggga atgtatatga tacgcctaat agtattaaaa atgtacgaaa aaatttattt
     9661 ggacatagtc caaaagatca agaagtagag gccttcccga tgtcagtatg tatgtctttg
     9721 tataataaaa atctgctaga taaatataga ctaagatttg aaaaaatttt atctgaagat
     9781 acaattttta acttgaatgc atatgatgtc gcaaataaaa ttgcttttac agaatataca
     9841 gattattgtt atagaaaaga agaacaagag tctattacaa aaagtttatc tccaaataag
     9901 cagacggaat ttaaagaatt tttaataact ctaaaagcta tagcatcgaa agaagattta
     9961 gaatgtcaaa tcagagcaaa acgtatggca attgattatt gtaggcttta tgtgggctta
    10021 gttgatgatg caaatttggg aattaaagag aaaagaaagt atataaaaaa atttattgaa
    10081 gatgagggat taattcaatg ttggaaggga tatcctacaa atacgctacc atttcaacaa
    10141 aaattttttc ataaaatgct tgaatgtaag tgttatacaa tattgttgtt gttaagtaga
    10201 attagaaaga taattaaaaa tatgaccaga aa
//
LOCUS       DARBEC010000096        10194 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.100, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000096 DARBEC010000000
VERSION     DARBEC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.100"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            121..627
                     /locus_tag="SCL80_002368"
     CDS             121..627
                     /locus_tag="SCL80_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEG1108210.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            644..1543
                     /locus_tag="SCL80_002369"
     CDS             644..1543
                     /locus_tag="SCL80_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEG1108211.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            1630..2514
                     /locus_tag="SCL80_002370"
     CDS             1630..2514
                     /locus_tag="SCL80_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEG1108212.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(2706..2777)
                     /locus_tag="SCL80_002371"
     tRNA            complement(2706..2777)
                     /locus_tag="SCL80_002371"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2742..2744),aa:Arg,seq:ccg)
     gene            2837..4171
                     /gene="rpoN"
                     /locus_tag="SCL80_002372"
     CDS             2837..4171
                     /gene="rpoN"
                     /locus_tag="SCL80_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEG1108213.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            4320..5411
                     /locus_tag="SCL80_002373"
     CDS             4320..5411
                     /locus_tag="SCL80_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEG1108214.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            5452..6453
                     /gene="gap"
                     /locus_tag="SCL80_002374"
     CDS             5452..6453
                     /gene="gap"
                     /locus_tag="SCL80_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1108215.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            6563..7753
                     /locus_tag="SCL80_002375"
     CDS             6563..7753
                     /locus_tag="SCL80_002375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEG1108216.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            7857..8612
                     /locus_tag="SCL80_002376"
     CDS             7857..8612
                     /locus_tag="SCL80_002376"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEG1108217.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            8728..10026
                     /gene="eno"
                     /locus_tag="SCL80_002377"
     CDS             8728..10026
                     /gene="eno"
                     /locus_tag="SCL80_002377"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEG1108218.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
BASE COUNT         3565 a         1886 c         2112 g         2631 t
ORIGIN      
        1 tttttgtcac accttctttt ttgtatataa aaatcccaag taaatacata aaaaataaaa
       61 aaattgaaac ttttagcgca ccttgtacgt catattaagt gaggcagaaa ggagtggaga
      121 atgattttta ctggatataa gaaaaaacgg cagatcgaaa aataccgtca cctgctgaat
      181 ggactcatcg aaactgatta tcaaaaaatg ttccgtatca cgatgaatta cgtacatgat
      241 aaagaagagg ccttagatgt gatgcaagat agttttcata aagccctgaa ctattttgaa
      301 aaagaaaaag agatcgagca ttttagtgca tggttttatc ggatattgat ccgcacggca
      361 ttagatagtt ggcgcagaca aaaaaggacg cctgtggact tatttgaaat tcaagatatc
      421 ccccaacagt tacctttaga aacttccgtt gcagagctcc atatgatttt agataaaata
      481 gacagtccgg aaaaagagat tctgatcctc tacttttttg aaggatttcg tttgaaagaa
      541 attgcgacta tcttggatat gaatgaaaac acagttaaaa cgaaaatgta ccgctcattg
      601 aatgctttac gtcaaatcct agaatgaagg agagaagagt gccatgtatt cccaaaaaga
      661 aaatttaaaa aaactaaaga aaacttataa agaacaagca attcccaaag ggcttaaaga
      721 ggagatccgc cagcgtttta ttatcgaaga gcaatcattt ataaaagcaa agcggagaaa
      781 gagacgtctc caagggttcg gagtatcttt ggctatcgta atgattacaa caagtagcct
      841 tctttttaat agtcaagttc gaagctttgc agaagattta cccatcttag gatcagtcat
      901 tgaattgatc ttaggcgaac gctttactga tcgatctgaa aaaatcgata ttcaggtacc
      961 gaagatcaat acacaaaatg agaaagaaaa taaaacaatt catggattaa atcaaaaata
     1021 ctttcgcgaa ggacaagcag attttgaaaa agctgaaaaa gaatatggaa attttgaaac
     1081 ggatcattac caagttttgg gagattatca gaaaatcgta gacgataacc ggtttctcgt
     1141 gattgaaagg cagatcaccc aaacagcagc agatagccat gttgaaaaaa gatacgacac
     1201 aatcgacaag aaaaacagtg tccagctctc acttccactt ttattcaaag atgacaccta
     1261 tctatctgtt ttgacaaaag aagtcaaacg gcagatggcc gaacaagtca aggaagatcc
     1321 atctaagtac tattggactg agcaagatat tcaagaaggg acgatagaaa aaccaacact
     1381 tgttacgcct acacgaagtt tttatttgaa caaagagcat caattagttt tgactttttc
     1441 tcaatacgaa attgctcctg gttatatggg tacaccagaa tttgtcattc ctaagtccgt
     1501 gacaaaaacg attttggcat cggaagatta tttggatcat tagacgagaa gtagttgcga
     1561 ctagcagttt caattttcaa gtaagtacaa gtaaataata agaagagaaa aagagaaaga
     1621 agagatatag tgaaaagaaa ttatttagtt atcagtatta ttgctttatc cgtgcttttg
     1681 tttttcgaaa ttacttcggt ggcctccttg atcaccgaag taaaaggaca aacacactct
     1741 tcggagcatt cgtcacaatc tttcaaacaa tcagaacaat caaccattaa aagccaatca
     1801 actgaaacag ctaacgtcaa agaagccatt caagaatcag cggaaaaggt acaaagtcag
     1861 tcatcacaat caacacaaga cagtacaaaa acgagccgag atccggctgt caaaagcgtc
     1921 gcaatcagct ttgatgatgg accaggagcg accacaacac cgcagcttct gcgaatctta
     1981 aaagaaaaaa acgtgcatgc cacttttttc gtcttaggag aaaatacggc gcagcatcct
     2041 gaaatcgtga agcagacagc tgaagcaggg cacgagattg gcaatcacac atacgatcat
     2101 cagaatctgg ccactctttc tgcccagtcg atgacggagg aagtgacgaa agcagacact
     2161 gagatcaaaa aagtcactgg caaaaccccc acttttgttc gtccgcccta tggttctgtc
     2221 acgaatgtag gagcaacgat catccagcga ccgatcatcg aatggtctgt tgattcagaa
     2281 gattggaaga caagaaatcc tgatttgatt ttgcagaaga tccaagcaac agtatacgat
     2341 ggggcaatta ttttattcca tgatatctat cctgaaacga ttcgtgccgt tcctcaagtg
     2401 atcgattatc tgaaagagca agggtatcgc atcacgacag ttggggattt attagggcat
     2461 ccaacggctg tcgagaacta ttatggcaga aacgatcatc ggccagttca gtaagtgaga
     2521 caagtatctt taataaataa cacattttaa taagtagttg aacacataac taccttactt
     2581 gcaaaggctt aacaatatct atacaggtac aacaaaaaac tgtccaccaa ttttctttgg
     2641 cggacagttt tcacttaaaa ataaagaagc aactattcgt aaattatgct aaaatagcag
     2701 tagaacgcgt cctgagggaa tcgaaccccc gtctcaagaa ccggaatctt acgtgatatc
     2761 cactacacta aggacgcaag cactgggata attgtaacta aacagataaa aaattacaag
     2821 gggattggac aaaaaaatga aatttgagca aaatttttca caaaaacagc agcagacaca
     2881 gaaactagcc atgacgcaac agttgcaaca gtccatccaa gttttacaat ataacagtga
     2941 ggagctgttg gcattcgttg agaatcaagc aatggaaaat ccattggtgg aagttgttga
     3001 gccggaatgg cagccagatt acataaaagc ttcttcatct tcttatgaag gagaagagac
     3061 aaattaccta aatcagattc ctgatacaaa agggtcttta tttgattcat tgatcgaaca
     3121 agtccatttg aattaccgag atactttttt gaggaaaatc gttctttatc ttgtagaata
     3181 catcgatttg aacggatttt tgacgattag tttagaagaa gccggaaaag agattggagc
     3241 tacgcctatc caaatgctcg atgcgttgac tctgattcag caattagaac cagctggagt
     3301 aggtgcacgt agtttgcaag aatgtctcat gcttcaaaca gaacgagatg attatgcgcc
     3361 ggaattagct tatatcgtgt tggaagaatg ttttgaggaa ctggtggagc gaaaatggaa
     3421 agaaatcgca caacgttttt ctgttgatct acatgctgtt cagcagatat tcgactattt
     3481 acaaacgttg agtccaagcc ctggccggat tttcgaccgt agcagcgaat tgtacattat
     3541 tcctgatgtc cgggtattag tggacgacga taaaaatgta caagttatct caaaccgaaa
     3601 aaatcagcca aatatacgct ttcaagagag ttacttcaaa caaatgtcac aacaagcaga
     3661 taaagaaact gaaaattatt taaaagaacg gaaacaggaa ttcgaatggt tgaagaaaac
     3721 gatcctacaa cgtggggata caattttgcg ggttgctcaa gtcattgttt cccggcaaaa
     3781 agctttcttt atcgacaagg aacgtccaat caagcctctt acgttaaagg aagttgcaac
     3841 tgagatagat gtgcatgaat ccacggtcag tcgtgcagtc aacgggaaat atttggaaac
     3901 agacttcggt gtatttgaat taaaaaaatt tttcactaca cgaattccta cgaatagtac
     3961 agaacagaca gaagaccttt cagcagatac agcgaagaaa aagctgcagg aattagtcga
     4021 tcaagaagac aaaaacaagc cgttgtctga ccagaaattg gttgaattgc tgaaaaaaga
     4081 tgagatagcg atctcaagac gaacagtagc aaaatatcgt gatttattag gaatcccgag
     4141 ttcatcaaaa agaaaacgtt atgataacta atcagtcaaa agactagaaa aagtatttca
     4201 ctttttctag tctttttagt tgaaaaacga gagcaaaact gttagactta cagtcgtgag
     4261 gttgagattt ccctttattg atgtgaaatt cgaatagtta atttcttttt ttatcgtata
     4321 tgggtcgttt taagtctaca ttggacgtaa agtgtccaac taaggagatc gctatgctgg
     4381 atgaaataaa aatgattgaa tctgttgcac cagatattat cgaagtgttg caagaacgct
     4441 ataaaatttt acgaaatatc tattggatgc agccgattgg acgtcgaagt ttatcggaaa
     4501 gtatggggat cacagaacga gtattgcgaa cagaaacaga tcttttgaaa aatctgaatt
     4561 tgatcgacac ctcaaaaagc ggtatgacgc tgacaactaa gggcgaagaa gtttatcaaa
     4621 gccttgaaaa cttcatggat cagcttttag gtacgcatca aaccgaacaa cagttggcag
     4681 aatattttgg taaccaacgc tgtatcgttg tttcaggaaa cagcgaagag cagaccaaag
     4741 tggctgatgc attcggcgaa gcgctgagcg aagccctaga ccgactactt ccagaaggcg
     4801 agaatatcat cgctgtgatg ggaggaacta ctatggctgt ggtagccgaa caattgtcta
     4861 atctggaaaa taagaaacgg cataacctgt ttgtccctgc aagaggcggg attggtgaag
     4921 cgataactgt acaggcaaat tctgtcagtg caagaatggc tgctaaagca aacggtaacc
     4981 accgtgcact ttatgttccg gaacaattaa gtctggcaac ttacaattct ttactcaatg
     5041 agccatctat ccaagaagtc ttaaacttga ttagtgaagc gaattgtgtt attcatagta
     5101 ttgggcgtgc attacacatg gcagcacgtc gtaagatgac tgaaaaagaa ttagtcatgc
     5161 tgaaacaagc aaatgctgtt gcggaatctt ttggctactt tttcaatgaa aaaggtgaag
     5221 tcgtttataa ggtccctaga atcggcatag aattgcaaga tttggaaaaa gtcccgatca
     5281 tcatggcaat cgcgggcgga aaatcaaaag ccaaagcaat tcgagcttat atgaaaaatg
     5341 caccaaaaca aacgtggctc atcactgatg aagctgctgc aaatgagatt ttaaaagggg
     5401 taacccttta aaataaatat ttttttgatt ttcataagga ggaaatctta aatgacagtt
     5461 aaagtaggta ttaatggttt tggacgtatt ggacgtctag cattccgtcg tatccaagat
     5521 gtagatggaa tcgaagtagt agcaatcaac gacttgacag atgctaaaat gttggcacac
     5581 ttgttaaaat acgacacaac tcaaggacgt ttcaacggaa cagttgaagt tcatgaaggt
     5641 tcattcaacg taaacggaaa agaagttaaa gtattagcta accgcaaccc agaagaatta
     5701 ccatggggcg aactaggcgt agatatcgtt ctagaatgta ctggtttctt cacttctaaa
     5761 tcagcagctg aaaaacattt aacagctggt gctaaacgcg ttgttatctc tgctcctggc
     5821 ggaaacgatg ttccaacaat cgtttataac acaaaccacg aaacattaac tggtaaagaa
     5881 acagttatct caggtgcttc atgtactact aactgtttag ctcctatggc taaaacattg
     5941 aacgacaaat ttggtgttgt tgaaggatta atgacaacta tccacgctta cacaggtgac
     6001 caaatgactc tagacggacc tcatcctaaa ggtgacttcc gccgtgcacg cgctgctgct
     6061 gcaaacatcg ttcctaactc aacaggtgct gctaaagcta tcggtttagt aattcctgaa
     6121 ttgaacggta aattagacgg agctgctcaa cgtgttcctg taccaacagg ttcattaaca
     6181 gaattagtaa cagttcttga aaaagaagta actgttgacg aaatcaatgc agcaatgaaa
     6241 gaagcttcaa acgaatctta cggatacaac acagacgaaa tcgtttcttc agatatcgtt
     6301 ggtatgactt acggttcatt atttgatgct acacaaacta aagtaatgac agttggcgac
     6361 aaacaattag ttaaaactgt tgcttggtac gacaacgaaa tgtcatacac tgcacaatta
     6421 gtacgtactt tagaatactt cgctaactta taagatagac aaacgtacaa gtgtaaatta
     6481 tagacatgta catttaaaag cggggaagca atcgcgcttc ttccgctttt tttgataata
     6541 agtttattca ggaggaaata aaatggctaa gaaaacagtg aaagatatcg atttaaaaga
     6601 caaaaaagtt cttgtccgtg ttgacttcaa cgttcctttg aaagacggcg tgatcactga
     6661 cgacactcgt atcaaagcag cattgccaac aatcaactac gttctagaac aaggcggaaa
     6721 agcaatcctt ttctctcacc ttggacgtgt aaaaacagaa gaagataaag aaggaaaatc
     6781 tttagctcca gttgcaaaac gtctaggcga attattaggt aaagaagtaa cattcgtacc
     6841 tgaaactcgc ggagaacaat tagaagaagc aatccgcaac atgaatgacg gcgacgttgt
     6901 cgtattcgaa aatacacgat ttgaagatat tgacggtaaa aaagaaagca aaaatgatac
     6961 agaactaggt aaatactggg cttcattagg cgatgtattt gttaacgatg ctttcggtac
     7021 agctcaccgt gcacatgctt ctaacgtagg tatcgcttca actggtatcc caacagttgc
     7081 tggattcttg atggaaaaag aaatcaagtt catcggtgaa gcagtagaag aaccaaaacg
     7141 accaatgatt gcaatccttg gtggcgcaaa agtttctgat aaaatcggcg taatcgaaaa
     7201 cttgattcct aaagcagata aaatcttgat cggtggcgga atgacttaca cattctacga
     7261 agcaaaagga atcaagatcg gtaactctct agtagaagct gataaagtgg aactagcaaa
     7321 agaattaatc gaaaaagcag gcgacaaact tgtcttgcca atcgacaatg tctgtgcacc
     7381 agaattttca aatgatgttg aaacacaagt aatcgaagga gacattcctg atggtttgat
     7441 ggctttagac atcggcccag catcagtaaa attatttgct gatacattac aaggtgcaaa
     7501 aacagttgtt tggaacggac ctatgggtgt atttgaaatg agtaactttg ctaaaggtac
     7561 aatcggtgtt tgtgaagcaa tcgctaactt agaaggcgca acaacaatca tcggtggcgg
     7621 agactcagcc gcagcagcag aacaattagg atttgctgac aaattcacac acatctcaac
     7681 aggtggcggc gcaagcttgg aattgcttga aggtaaagaa ttaccaggac tagcagcaat
     7741 caacgataaa taaacgacca agtttgctca attctgacga gtaatcgatc aaaaggtgaa
     7801 gggagttttc atgactccct agccattttt caataaatta aggagtgtgt tcctcaatgc
     7861 gtaaacctat tatcgcaggt aactggaaaa tgaacaaaac tgcatcagaa gcaaaagaat
     7921 ttgctgaagc agtaaaaaac aaaatccctt caaatgacaa agttgattca gtcatcggat
     7981 ctcctgcatt attcttacaa gaattaatga catcagctga aggtacagaa ctaaaaatct
     8041 cagcacaaaa ctgctactgg gaaaactcag gtgcgttcac tggtgaaact tcaccagcag
     8101 ctcttgctga tctaggtgtt cattatgtga tcatcggaca ttcagaacgt cgtgaatatt
     8161 tccatgaaac agacgaagac atcaataaaa aagcgaaagc tatctttgca aatggtatga
     8221 caccaatcct atgctgtggc gaatctttag aaacctatga agcaggcaaa actgctgaat
     8281 ggatcgaagg acaaattaca gctggtttag ctgatctttc agctgaacaa gtgtcaaaca
     8341 tggttattgc ttatgaacca atctgggcaa tcggtactgg taaatcagct gacgcacaaa
     8401 tcgctgacga aatttgtgga gttgtacgtc aaacagttga aaaactttat ggaaaagaag
     8461 tatcagaagc agttcgtatc caatacggcg gttctgtaaa accagaaaat attgctgaat
     8521 acatggcaaa agaaaatgtt gacggtgctt tagtcggtgg cgcaagcttg caagcagatt
     8581 cattcttgtc attgttagac gctgtaaaat aattgttgaa aatttgcgaa aaatgcaaga
     8641 aatgtttgca ctaatgagca aaattaacta aaatcgtata tgagggaaat cccttaggaa
     8701 aaaacaaact caaaggagag acaaaacatg tcaattatta ctgatgttta cgctcgcgag
     8761 atcttagact cacgtggtaa cccaacaatc gaagtagaag tatacacaga aagcggagct
     8821 tttggccgcg gaatggttcc ttcaggtgct tcaactggtg aatacgaagc agttgaatta
     8881 cgtgacggag acaaagctcg ttatggcggt aaaggtgtaa ctaaagctgt cgacaacgta
     8941 aacaacatca ttgctgaagc aatcattggc tacgatgttc gtgaccaaat ggctatcgac
     9001 aaagctatga tcgctttaga cggaactcct aacaaaggta aattaggtgc gaacgctatt
     9061 ttaggtgttt ctatcgctgt tgcacgtgca gctgctgact acctagaagt acctttatac
     9121 cactacctag gcggattcaa cacaaaagta ttgccaacac caatgatgaa catcatcaac
     9181 ggcggatcac atgctgataa ctcaatcgac ttccaagaat tcatgatcat gcctgttggc
     9241 gctccaacat tcaaagaagc tctacgtatg ggtgctgaag tattccacgc attagcttca
     9301 atcttgaaag ctcgcggatt agcaacttct gttggtgacg aaggtggatt tgctccaaac
     9361 cttggttcaa acgaagaagg ttttgaagta atcatcgaag caatcgaaaa agctggctat
     9421 gtacctggta aagacgttgt tcttgctatg gatgctgctt cttcagaatt ctacgacaaa
     9481 gaaaaaggtg tttacgtact agctgattca ggcgaaggcg aaaaaacaac tgacgaaatg
     9541 atcaaattct acgaagaatt agtttctaaa tatccaatca tctcaatcga agacggatta
     9601 gacgaaaacg actgggacgg attcaagaaa ttaactgacg tattaggcga caaagttcaa
     9661 ttagttggtg acgacttgtt cgtaacaaac actcaaaaat tatctgaagg tatcgaaaaa
     9721 ggaattgcta actcaatcct tatcaaagtg aaccaaatcg gtacattaac agaaactttc
     9781 gaagctatcg aaatggctaa agaagctggc tacacagcag ttgtatctca ccgttctggt
     9841 gaaacagaag attcaacaat ctctgatatc gctgttgcaa caaacgctgg ccaaatcaaa
     9901 actggttctc tatcacgtac tgaccgtatt gctaaataca accaattact acgtatcgaa
     9961 gaccaacttg gtgaagttgc agaatacaaa ggtttgaaat ctttctacaa cttaaaaaac
    10021 aaataattct tttctaatga agatacatga tcgactattg aaagatagtc gagtatgatc
    10081 acttagttaa gtaatcgaac tctctacttg ctgaaatatc ggctctttgt caataaggac
    10141 tgatgagttg tgcaaaatca aatctgagtc agaatgaacc acattctggc tcag
//
LOCUS       DARBEC010000097        10086 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.101, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000097 DARBEC010000000
VERSION     DARBEC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10086
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.101"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(247..759)
                     /locus_tag="SCL80_002378"
     CDS             complement(247..759)
                     /locus_tag="SCL80_002378"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEG1108219.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCL80_002379"
     CDS             complement(773..3070)
                     /gene="recJ"
                     /locus_tag="SCL80_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEG1108220.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(3212..3655)
                     /locus_tag="SCL80_002380"
     CDS             complement(3212..3655)
                     /locus_tag="SCL80_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEG1108221.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(3656..4444)
                     /locus_tag="SCL80_002381"
     CDS             complement(3656..4444)
                     /locus_tag="SCL80_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1108222.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(4441..5382)
                     /locus_tag="SCL80_002382"
     CDS             complement(4441..5382)
                     /locus_tag="SCL80_002382"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEG1108223.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(5559..6146)
                     /locus_tag="SCL80_002383"
     CDS             complement(5559..6146)
                     /locus_tag="SCL80_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEG1108224.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCL80_002384"
     CDS             complement(6367..7674)
                     /gene="obgE"
                     /locus_tag="SCL80_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEG1108225.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            7968..8978
                     /gene="gap"
                     /locus_tag="SCL80_002385"
     CDS             7968..8978
                     /gene="gap"
                     /locus_tag="SCL80_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEG1108226.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
     gene            complement(9063..9998)
                     /locus_tag="SCL80_002386"
     CDS             complement(9063..9998)
                     /locus_tag="SCL80_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEG1108227.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT         2778 a         2298 c         1746 g         3264 t
ORIGIN      
        1 ttttacgggg gtgtgacaaa aatcggtcac atcctttttt tcgaataaac ggtgttctaa
       61 agccagtttt tcggtattaa gtcaaattgc ggaaaaagtg attttttaaa cttctactgg
      121 tctttgcttg tttccattta taaaaaaata gcgaccctca aaagtcagat agagaatctg
      181 acttttgagg gtcgctttaa aatcgcttct ttaagaataa catcaagaat agcttctttt
      241 ttctgcttaa tattccatta atgtaatgat gtcatatcct ttgattttgt cgcgtccatg
      301 gagatccatc aattcgatca aaaatgcgca gcctacgact acaccgccta attgttcgat
      361 cagttcgatc gtcgctttga ttgttcctcc agtagctagc aaatcgtcac aaatcagcac
      421 acgttgacct ggtttaatgg catccttatg caatgtcaat gtatctgttc catattctaa
      481 tccataagtc acttcgatgg tttctcgagg taacttccct tttttgcgga ctggggcaaa
      541 accgactcct aactcataag caacaggaca tcctacgatg aatccacggg cttctggccc
      601 aactaccata tcaattcgtt tttcttttgc ataatttacg atttgtttag tagcttcccg
      661 ataagcgtca ccatctgcca tcagtgggga aatatctcga aacacaattc cttttgacgg
      721 atagtcggga atactggcaa tataatcttt taaatccact ctttgtttcc tcctaggttg
      781 ttaaccattt ctttatggta ggaatatcgc tcaaaagcaa aaattcctca actttgattt
      841 tattgagccg tcgctggtat aatctgctct cagtcaaagg atgactttct gggctagcga
      901 ctttttgtaa aacaccgttc tctattgtaa caaatttcaa ttcaaaaaac acctgtatca
      961 taaaaattaa taatttttca gggatacgca aatattgagc tattgctttt attttatgac
     1021 gtatatcaat tcgttcttga gaatggatca atttaaacaa ttttgcgtac tgttctcttg
     1081 tgcctacccc attcaaatag gcctcatcag gtgaaatccc catcagataa acacgattga
     1141 atgaataaga ccgaaaaact tctttcagat ccgctaaatc atctggacaa tccataacaa
     1201 ctagagagtt tggttttctt ttttcaatta ttgcctttac agaatctagt ccttcgaaaa
     1261 caatcgcagc ttcctggata atctcaggca cgaatttcaa cgtcgaagaa tcaaatgcta
     1321 aataaaggct accttccgac gttcggctct gttcacggaa acgttttgcc cgccaatcaa
     1381 atacttgcag accacccaca gaaaaatcag ataccatcag ctgcggtttc ttacgcccgt
     1441 tccattcatt gatcgaaagc tttcctacca catctacctg attattcagc agttcttctt
     1501 cttgagtacc aaaaccaaaa gcgaccgcat caagctgact cgatgcatcg gaaaggacaa
     1561 atttcagatg ctgttggttt gctccgattc ttttgatatt ttctgcttgg acttgtcgaa
     1621 ataaaaactg aggcaaagga ttatccgtac caaatggcgc caatagtttc agctcatcaa
     1681 ttcgctctac tgttacttca tttggcagca atacttcatc aattctcaac tcaggccctc
     1741 tcatcaagtc aatttgatga tcgactatat attggttcat cttctcctgt agtatagtga
     1801 cattttcact tggcatcgtt aaaccgactg ctgcatgatg tcctccgaag taagtaaata
     1861 aatctcgcat ctggtcaagc atttcaaaaa gattcaaggc ttcaatacta cgtccagatc
     1921 ctttagctga tccatcttct tttaaagtaa ggacaagcgt tggtttaccc gtttcattca
     1981 ttattttccc agcaacaatg cccaaaacgc cttcatgcca tcctcggttt accaatagat
     2041 ggatatgatt ctccttattg accatcgcta atgcttcttg tgttatctgc tctacgattg
     2101 cttttctttc ttcattgatc tcattcaatt tttttgcttg aacggatgct tcctcttcat
     2161 caaacgtcgc aagtaacgag acggcaggac gtggatcgcc catccgaccg attgcattta
     2221 gtctgggagc aatcgaaaaa ccgatcgttg tttcgtctgc gtccctcatt tttacgccgc
     2281 tttcttcaaa tagcgcctga agtcctatcc gttctgaatg atgaatggca tcgattccta
     2341 atgaaacaag gattcgattc tcatcagtca atgaaactaa atcggcgatc gttcctatcg
     2401 ctactaaatc caaaaattct gcaggcggtt cttccaataa tgcagaagct actttgaatg
     2461 ctactccgac tcctgccaaa tctggaaatg gatagtgccc cgctggatga cgagggtgaa
     2521 caatcgcata agcgtctggt aaattctcag gtagttcgtg atgatctgta atgatcacgt
     2581 ctacaccagc ttgctgagcg tatgctaccg catcatttcc tgctaccccg ttatcaacag
     2641 tgatgatcaa ctggattcct tcttcgatct tttcttggta taccgattgg tttggtccat
     2701 acccatgctc aaatcgattt ggcaggaata cttctacttc tgcaccaagt aattccagtg
     2761 tttctttcat taccgttgca ctggtgatcc catctgcgtc ataatcaccg taaacgagga
     2821 ttttttctcc actgattaca gcttcttgga ttcggctgat tgctttatcc atatcgtgta
     2881 tcagatacgg atcatgtaat tcttgttcct ttggatgtaa aaatcggttg atatcctttt
     2941 cctcatggta acctctttgc cacagtaact taccgacaaa tggagataat ttcagttcac
     3001 ggatcatttg gagaaaggat tcagaaggct ctgtctcgct taacttccaa tcaaaatttg
     3061 cttgtctcac tttctcactc ctaacttagt tattatagca aaggaaatca gcaaaaaaaa
     3121 gcagaaagaa agaataaaaa cagatcagat ccgctagaca gatgttcagc aggacctgat
     3181 cttttgtacg accatttatg aaatatccct attagtcaaa ataattgaag ctttgtttgg
     3241 gcgattgagt tttttgttct tccggcgctt tcaattgact gatttgtgcg gttttcgctt
     3301 gtaactccgc ctgataagca gcttccaact ccgcacgttc gtttctaaac tcgcgttgct
     3361 gttcttcttt tgcttcttca attttctttt gaagttctgt ttgatattct gacatttcct
     3421 gagtcagctg tttgatttgc tttttttgct gccatagtgt tgccgaagca gtcaaaaata
     3481 caatcagtgc accaatcagc gcagaaccga tgatcactaa gatcaatggc gcagaaaagg
     3541 aagtgaagcc aaaacttaca ggaacatttt tattgttcaa tacagcaaaa agaacaataa
     3601 tcaaaactaa aacaaatcca agaatcacat tgctttgctt tttcaattcc ttcacctact
     3661 ttttgttaaa aattccgctg gctaaataat caccaatcgt tggaaacagc gtataaaatt
     3721 ttgatgcaac ttctaaaata aacggttgat tgatttcccg ttttggatgt ctcatattgc
     3781 ggacgatcat ttttgccaac ttggcaggtt ctaaaacgat ttgtccgact ttttccaaat
     3841 agcttccgct tggatcagcc ttatcgaaga aatttgtctc gattggacct ggattcactg
     3901 tcgtaacagc tacgccaagt ggtttcaatt ctaatcgcaa agcgttggag aagccaagga
     3961 ctgcaaattt cgtggcagaa taaaccgttg attttgcagt tgccattttt ccagccatag
     4021 aggcgatatt aatgatatga ccttgtcttc tttctgccat atcgatcgca aatttttgtg
     4081 tcaaaaccat cattcctaag atgttgactt caaacatatc gtatgctttt ccaaggtcga
     4141 atgtaaggaa gtcttcgaaa atcccaaaac ccgcattgtt taccagaata tcgacgcgcc
     4201 cgacttcttc catcaccttt tcatataatt cgtccacgct ttcaggattg ctgacgtcta
     4261 ccttgaaagc ataagcctct ttgccgctca agcgtctgca ctcgtctctg acaccttcta
     4321 caaatgccat tcttcgtgcg caacttataa cgattgctcc ttgctttgct gcttcataac
     4381 agatttgtgc gcccagaccg cctgaactac ctgttactaa aacaacttta tcttctaaat
     4441 tcatgcctct tcctcctcgc gaaatggaat ctcaatgcta tcaaaatctt tcataatcgt
     4501 cgtgttttca aacacttctt gtgcttcttt ttcaagctct aaagctgctt ttcctaaata
     4561 tctcgcactg atatgagtaa gtagcagccg ttttgcctgc gcttcttttg caacttcagc
     4621 agcttgatga gtcgtggaat ggaaatagtt gtgcgccatt cttgcttcat ctttattaaa
     4681 tgtgctttca tgtaccagaa tatcgctatt ttccgcaagc acaacggaat tatgtgtttt
     4741 tctcgtatcg cctaaaatag tcacgattcg tcctttttta tctggaccta cgaaatcttt
     4801 tccattgatc gtccgcccat cttctagttg gatcgtttca ccttgcttca gtttgccgta
     4861 cagtggtcca gctggaatac ctaattcttt tagacgatcc acctgcaatt ctcctttatg
     4921 atcatgttca accacacgat aaccaaaact gtcgatccca tgatttagtt tttttgtata
     4981 aacagaaaat tgctgatcag taaaaatcgg ggctgtttca tttagctcaa tgaatttcag
     5041 cggataactt aaacgcgttt gcgaaatacc taaagagact tttatgtatt cctctatccc
     5101 ttttggcccg taaatctcta atggcgtatc cccaccttga aatgaccggc tgctgatgag
     5161 cccgggaagt ccaaaaatat gatcgccgtg taaatgtgta atgaaaattt taccgatttt
     5221 tctcggacga atagtcgttc gcaaaatctg catttgcgtg ccttcgccac agtcgaacaa
     5281 ccaaacttca ttgcgctcgt ctaaaagttt caatgctata cttgtcacat ttctatgttt
     5341 tgcaggaaca cctgcacctg ttcctaaaaa ttgtaattcc ataatctacc tgactttctt
     5401 taagttcctc tttaatttta gggtaaaaag aagggataat caaccgtaag tcgagaatgg
     5461 gttcaaatta aaaataacgt tctaaagcga tagcgttttc tttgaaaatt actataaaaa
     5521 cagcagcgaa cccgtttgag atcctctgct gttttttatt aatttgaaga tccagaactt
     5581 tcattgtcgt tggaagtttg cggatgtttg tccttccaag tcttttgtgc agcttctttc
     5641 gcatgatttt cagcttcttt cgcatcatgc acatcttttt cgacatcttc ataatccaag
     5701 cgagtaattt ctccgcctaa ttccatcatt accaattgat tcagtgggcg attgtcctct
     5761 tcatcagcaa tcaagatcag tcctgtagct ccttcaccga ttttatttcc tacaaagtcg
     5821 aacacggtct gggcatcttt tatttctttg gcatcttgtg tcgcaccgat cattcctccg
     5881 gcaaaccatc ctaataaaat ccctagcgga ccacctaata caccaaccaa cattccgatt
     5941 aatccacccg tagaagattt gttattaccg gtaaagtcga taaaatcttc tattttgaat
     6001 tgatgtgtcc cattaggatc atggctgaca actgccatct gttctccttt aatcgaacga
     6061 acagcatgta actttttgat ttctgaaaat gcctgatatg cctggctttc tacttgaaaa
     6121 tttaaaatga ttactcgttt gctcattaaa aacgcccctt tcataccttc ttgttattta
     6181 ttttaccttt aattgaaaaa taagaaaaga aaagcactct ttttttccaa taaaataggg
     6241 ctgtgacaaa agtcgtatca cagctccttt ttccgaataa acggcgttct tgaagctgat
     6301 cacttctgtc acaacctccc ttaaatctga atctatattt tttggtattt gtttttattt
     6361 gcttgcctat tcgacaaatt caaattcaaa ctcaccgata cgtacaaggt cgccatcttt
     6421 cgcaccgcgt gcccgaagag cttcatccac acccatacca cgtaattgac gagcaaaacg
     6481 catcactgtt tcatcatgat cgaaattcgt catttggaat aatttttcga tcttctcacc
     6541 agataagatc cacgttgcat ctgaatcacg atcgatttgg aactcaggac cttctggatt
     6601 gaatccatag tgtacagttt cttcttccag ttcttcttcg tacaatggga attctggcgt
     6661 tacttctaac aaatctgctg tagcattcaa taacgcgtcc aatccttgtc gagtcactcc
     6721 agaaattgga aatactggga tgtcatcagc aaattcatct tctttttcct tgttcagctg
     6781 ttctttgaat ttcactagat tttccggtgc atccggcata tccattttgt ttgccacgat
     6841 gatttgcgga cgctctagca agcgtaagtt ataagtagat aattctttgt tgatggcaag
     6901 atagtcttca taaggatctc gtccttccat cccactcata tcgatcacat gtaaaattac
     6961 tcgtgttctt tcgatatgac gaaggaattg tgtacccagc ccaacacctt gcgaagctcc
     7021 ttcaatcaag cctggcaagt ctgctgcggc aaaacttctg ccatcacttg tagtaaccat
     7081 tcctagatta ggtacgagcg tcgtgaaatg ataagcgccg atttttggac gagcagatga
     7141 aataacggat agtaaagtcg atttcccgac agatggaaaa cctacaagtc caacgtctgc
     7201 caatactttc aattctagtt cgatttttcg ctcttgtcct ggttcaccgt tttcagcaat
     7261 ttccggtgca ggattccgag gagaggcaaa acggatattc cctcgtccac cgcgtccacc
     7321 ttttgccaca actagtgttt gaccattttc aattaaatct cctaaaagag caccagtctc
     7381 cgcatctcgg acagtggttc cttgtggtac tttaacatag gtatcttctg aaccacgacc
     7441 gtgcatccct ttactcatcc cattttcacc tggctgcgct ttgaaatggc gattgaaccg
     7501 aaaatccatc aatgtacgta acccttcatc gacgatcaag atgacatcgc cgccacgacc
     7561 accgtctccg ccagccggtc cgccgtcagg aacgtatttt tcgcgacgaa atgcaaccat
     7621 tccgtctccg cccttgccag ctttcacatc gatcgttact tgatctaaaa acatggacat
     7681 aattaagtcc tcctattatt catttattta tttgactgcc tgaatttcca caataaacaa
     7741 aagtcttact tattttatcg gtgaaatctg attttgtcta tcatttcagc aaaaagattg
     7801 tttaccttct tcttttttta tcatttggta aggtttccaa ccttttttct gctaccaagt
     7861 ataatttttt gacaaaatat tctttgcgta gcagaataac agtgtattaa ttgtattaat
     7921 tgaggatgcc gtttcacagc acccctaata aaggaggaac ttttgatatg acagtaaaag
     7981 taggtattaa cggatttggg cgtattggcc gtctggcttt ccgccgcatc aaagaagtct
     8041 cagatgatat tgaagtagta gcaatcaacg acttaacaag tccaactatg ttagcacaat
     8101 tattgcaatt tgactctact catggtactt atcctggcac agttactgca acagaagact
     8161 caatcgtagt ggatggcgaa gcaactcgtg tttatgcaga acccgacgca agcaaaatcc
     8221 cttgggcaaa agaaaacggt gtagatatcg tactagaatg tactggtttt tatacatctg
     8281 aagaaaaagc aaaagcgcat ttagatgctg gtgtcaaacg agtagttatt tctgccccag
     8341 ctggtgctat gaaaaccatc gtctataacg tcaatgatga tacattagat gaaaacgaca
     8401 aaatcatctc agcaggttca tgtacaacaa actgtttagc acctatggct tatttcttaa
     8461 ataatgaatt tgggatcgaa gttggtacaa tgacaacagt tcatgcctac acttccacac
     8521 aaatgctctt agatggacct gtaaaaggcg gtaatttgcg tgctgctcgt tcagctgctg
     8581 acaatactat cccacattct actggtgctg cgaaagcaat cggcttagtt atccctgagc
     8641 tacaaggaaa attacaagga cacgcacaac gtgttccagt cgttgatggt tcattaactg
     8701 aattggtttc agtattgaaa acaaaagtaa ctgctgatca agtaaacgaa gcaatcaaaa
     8761 aacacacagt tgacaatcct tcattcgggt atgatgaccg tcaaatcgtt tctagcgatg
     8821 tcattggaac aactcaaggt tcgatctttg acccaacgca aacagaagta acaactgctg
     8881 gtgatttcca acttgttaaa actgttgcat ggtatgacaa tgagtatgga tttacttgcc
     8941 aaatgattcg cttactagaa aaattcgcta acttgtaatt tgatagatca atcgataaaa
     9001 gaagcctttg actggatcca gtcaaaggct tctttctttt catcccctac ttttaaactc
     9061 gttcaatgaa catattggct taaaaattgt tggactactt gctgatattt ttcaggatct
     9121 gtcttgtatg cttccgcatg ctcggctcct tttacaacat acttttcttt tggtcccttc
     9181 gttgcacgat agaccttatc taacatttca aatggtacaa atgtatcttc gtctccatgt
     9241 ataaagagca ttgggcgctg gtttttcttc aattgttcca ctgcatttgc ttcaccgaaa
     9301 aagtagccag cacggacttt tgtcactaga ctcgttactt gcataagcgg aaatgctgga
     9361 agactaaaca tatcgtctag ctgataagca agctcatcat gaacggaaga ataaccgcaa
     9421 tcttcaatga ttgctttcac attatctggt agtttttctc cgctagtcat cataactgtt
     9481 gctgctccca tgctgacacc gtatagaacg atttcttgcg attttccatt gttttctagc
     9541 actttgttta tccactgtac atagtcttta cgctctggcc atccaaatcc aatgtaatcc
     9601 ccttcgcttt ttccatgtcc tcttgcatca ggaacaagga cattgtaacc catatcatga
     9661 aacatctttg cgtaattggt catcgtttca gcgtttccca tatagccatg agccacaaga
     9721 gctgtttttt cacttttcgt ttcagcagga agataaatcg ctgataattt cagaccgtct
     9781 tttgaagtga tggtccaatc gcttcggttt tcttttgaac taaaccattt atgtgcatcc
     9841 gtttctttgg atgtctgtgt tgtaccaggt gtatcttcgt ctaaaaagtc tttctcagaa
     9901 ggcacaacag cataattata aaaataattc cctgcaaaca ctaacgcaac aatcagagct
     9961 aaagcgacgc ctaaaaggat tttcatccaa agtttcataa atcatccatc tcatttctct
    10021 tttttcatat aaccttgaat tagtataatc aaagaatctc tactatgcaa tgttcatttt
    10081 tataac
//
LOCUS       DARBEC010000098        10051 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.102, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000098 DARBEC010000000
VERSION     DARBEC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10051
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.102"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(515..739)
                     /locus_tag="SCL80_002387"
     CDS             complement(515..739)
                     /locus_tag="SCL80_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108228.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            930..3707
                     /locus_tag="SCL80_002388"
     CDS             930..3707
                     /locus_tag="SCL80_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEG1108229.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            3788..4207
                     /locus_tag="SCL80_002389"
     CDS             3788..4207
                     /locus_tag="SCL80_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEG1108230.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            4219..4689
                     /locus_tag="SCL80_002390"
     CDS             4219..4689
                     /locus_tag="SCL80_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEG1108231.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            4706..5485
                     /locus_tag="SCL80_002391"
     CDS             4706..5485
                     /locus_tag="SCL80_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEG1108232.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            5472..6293
                     /locus_tag="SCL80_002392"
     CDS             5472..6293
                     /locus_tag="SCL80_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEG1108233.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            6310..7359
                     /locus_tag="SCL80_002393"
     CDS             6310..7359
                     /locus_tag="SCL80_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1108234.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            7372..8376
                     /locus_tag="SCL80_002394"
     CDS             7372..8376
                     /locus_tag="SCL80_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1108235.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            9009..10028
                     /locus_tag="SCL80_002395"
     CDS             9009..10028
                     /locus_tag="SCL80_002395"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEG1108236.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT         3370 a         1655 c         2096 g         2930 t
ORIGIN      
        1 tatagatgaa actctattta cttctttttt aatttgttct tgcaatcttt tttgctacgt
       61 aatttttgta aaaaagtggg ttgagagaaa tctaattgat tttcaaagaa ggagtatgaa
      121 aatgagaaaa atatttattc gtttattgtg ggtactatct tagaggaata aataatagtg
      181 gagaaaacta tggtttggcg cagattctat caggtttgat ggaacgaaga caacctcccg
      241 acatcaagtc aaaagctact tgagattttg atctgaaaat aaaaaaagaa aacaaggtag
      301 gaacatcaat gtttctacct tgttttcgta atcaatggga aaaaactaag agtggtgtac
      361 gtaatcaata aaaaggatgg tgacaacagt acctctttac tctctgaaag catcataact
      421 cgtttttcct cattatgcca ataaattgtt gttataccac atataaggat ttgtaatagc
      481 taaaaacaga caggaaagtt gaaatctgtg aatcttattt tgtatggaag gatttataga
      541 acgcaaaaat caagataaag atgaataaaa atcctgtttc ggctatatat tgcgtatcta
      601 cgccaataaa acgagtttgt tctgtcatag ccatataaag caaaggcaaa aatacaacta
      661 cagcgagata agatccgatg atttctcgaa aagttttcca ggaagtatgt ttcctgtttt
      721 tatatttatc cgatttcatt gaatagtcat agcctcctta ttctttcata tgttgaaatt
      781 ccagtataaa aactggaagg aactgtacgt tttttagaat aaaaattata agggataagt
      841 aggctgctgt ctataataag atatattttt caattcttta ttaaagattt tattgacaaa
      901 atgaatccgt tttctatgat ggaggagggg tgaataagtt gttgaagaag gaaattttat
      961 gttacttaga gaaccagaca gcctttttcg atttagaaca tatgaacgag gtctttacag
     1021 cacaaaggtt agctgatcat tttggggtaa aaagaaacac agtcagtcat tatttgaacc
     1081 agttaaatga tgagcaacta ctagtcaaaa tcaattctcg accagtgatt tatttccaca
     1141 aagaagcttt tgaaaaacaa ttttttaagt tacgcacgaa tttttatttt tctatcgatg
     1201 atctcaaaga ggaacagcct tttttcgcgc aaccaaaaga cttattctct tttatgatcg
     1261 gatacgacgc cagtttgaaa gaaagtatcg aacaaatcaa aacagctctt tactatccag
     1321 atggcggatt accacttttg attacaggcg aaagtggcac aggtaaaagt tatttagtta
     1381 atttggtcta tcagtattgt ctgcttcatg atctgctaga agattctgca ccattcatca
     1441 cttttaattg tgcacaatat gcagataatc ctgaactttt gactagcaat ttgtttggac
     1501 atgttaaggg agcgtacact ggtgcagaag aaaataaaaa aggggctttt gaagcagcag
     1561 atggtgggat cttgttttta gacgaggtac atcgcttaac accagaaggt caggaaaaac
     1621 tgttcaccta tcttgatcaa ggtgtgattt atcgtatggg agaacctaat ttgtcacgcc
     1681 ggatcaaaac acgtctcttt tttgctacga ctgaagagat tactagtcat ttcttaacaa
     1741 ctttcattcg aagaatacct atccgaatcg aactacctgc acttaatcag cgtagtcgta
     1801 atgaacggct agaactgatt tactcttttt ttctcgaaga acaaagaaaa atggggagcc
     1861 ctttgtcagt cagtggacca gctttgtcac ttttaacaag tcagaaatta tccggaaaca
     1921 taggagagct gaaaaatatc gtcaaagttt cagcagcaaa agcatatgct gaacagcgtg
     1981 aacagcctga aatccatgtc acgatccatc atttgcaaaa agaactactt gcgcagccta
     2041 ttactaaaaa tacagcacag caaggaatct atattacgca agaccagacc ttagagcaat
     2101 taatggagaa gcaacagccc gaacagcagc gaatcgttaa aagttttgag caaatcctaa
     2161 ttgatttcaa gaaaaataac tgtctgctat attcttgcga aggaaaatta aagcaagaag
     2221 tgattcaatt atttgatttt ttgctgtttg agaccagtag acaagaaaaa cacgagctgc
     2281 tggtttattg gacacaatct atccgagaga cctttcgaca aatggaaact gcctatcaaa
     2341 tcaaattcga tggaaacagt gtctatgcct tgagttacta tctctatcag cgaagcacta
     2401 tcaaatggtt accggaagaa catgatatta tccaactaat caaagaatta gaacaacaag
     2461 tttctcagtc ttatccatct agttatcact atgtccaacg aattctggaa ttaagtaaga
     2521 tcaaattaga tattgaagac acaagtatgg atcggatttt actgacgatc tatctaaaaa
     2581 aagcgaaatg gtcaaaagaa aaacgactgc caaaagcgat gattgctgct cacggttatg
     2641 caactgctag cagtatggca aatgtagtaa atcgtttatt acaagaggat ctatttgaat
     2701 catttgatat gccactagat gtcacaccac agcagattgc ttcagaaatc cttgattatt
     2761 gcgaaaacag cgatgtttca aatggattga tcatattagt agacatggga tctctgaaag
     2821 aaatccatca gttttttaaa aaacagttat ccgtaccgct tttgattttg aataatgtga
     2881 cgacaccttt agcaatcaca gtaggggaat gcctgcaaaa aaatgtatca ttagaagaga
     2941 tagcagaaga gactgttcga cagatccagc cagaatggcg actgctctat ccagaggaaa
     3001 acaaacccaa agcactcatt actacttgct tcactggaat cggtacggcg gtccacttaa
     3061 gtgaactgct agaaaaaagc ttacctacgg cttgtcagtt gaaaatcatc ccatatgaat
     3121 atcagcaatt aaaagataag aaaaacagtg acccattatt ttccatatac gaagtcgtag
     3181 gaatgattgg caccacagat cctgatatca ccaatatcga ctatctttca ttagaagacc
     3241 tcatctcggg ggaaaagatg tctgtactag ctgaatggtt ggacactact atgaatgctt
     3301 cagagaagga aatattcaat cagcgcatca ttcgtaattt ttcattggaa aaagtactgg
     3361 attccgtgac tattctagat acagagaagg tgatgggaga aatcgatcat tttatgagag
     3421 aattagaggt gcagttgaat cgaagcatca gcaatcccaa aaaattagcg ttgtatgtcc
     3481 atgtgagctg tctcattgaa cgattgatcc gacaaatgcc aatcgaaacc tatcaaggat
     3541 tagagaaatg gaagcagtgt caaaaagaag ggttatctgc tattaaatct gcgtttagtg
     3601 tcattgaaga gcattatagt gtcatgatcc ccaattctga gattgcctat atctatgata
     3661 ttttgagtga agatacagag tttctctcga tagaagaaga attttgaaca taagcaagtt
     3721 gttgccacta attttggcat gcaacttgct tttttagtat gtgtaagtga aaagaggaga
     3781 tgaaaaagtg gatagagaaa ttatcttagc ttcccatgga aaatttgctt cagggatatt
     3841 agattcatta gagctgatct atgggaaaaa tcattctatt actgtattcg attgctatac
     3901 tgatgagaat tttgatttag cggaaacagt aaggcagctt tttatgaggt acaaagataa
     3961 agaaatcatt gttctgacgg atctttttgg cggaagtgtc aacaacgaat tcttacaata
     4021 tatcaatcgg gatcatgtct atctcgttgc cggattgaat ttgccactat tgattgagct
     4081 agtatctcgg ttggacgctg aagtagagac aactgttttg atccagcagg tgctaactga
     4141 ttcaaaaaaa atgatccagt tttgtaacga gcggctcaat cagaaaatag aagaagacga
     4201 attttaggag gaagaaaaat gattgtattg acaagagtcg accatcggtt attacacgga
     4261 caagtagctt tttcatggac acaaacaata ggcgcagact gtattttgat tgctaatgat
     4321 gatgtaccta cgaatgagat acgaaagaca acgatcaaat tagccaagcc acaaggagtc
     4381 aaactagtga tcaaatcaat tgatgactcg attgccgcgt tgaaaagcgg ggtgacagat
     4441 aaatataagt tattcatcgt agtggaatcg atcgaggatg catataagct ggcagaagcg
     4501 tatccagaaa tcaaaaccat caacttagga gggatcaagg caaaacctgg tactcgcagc
     4561 atcggaaaag cggtaaatat tttaccagaa gaggaggaac gcttgaaacg attaattgac
     4621 aaacaaatag aagtagaaat ccgtcaagta ccagcagata aaaaagtgga tgtaagagaa
     4681 gttttgtaag aaaggaagaa cctcaatgat tgtacaagca gtcttattag gaattgttgc
     4741 atttgtcgca caatcagaat atgcactagg gacatcgttg ctttctcgtc cgattgtgac
     4801 ggggctgttt acaggaatcg tgttaggtga tgtaaaaact gggatcatta tgggcgcaac
     4861 tttagagtta gcatttatcg gatcgttctc agtaggtgct tcgattccac ctgatgttgt
     4921 gaccggtggg attttaggga cggcttttgc tattacagct ggagccggta cagaaaccgc
     4981 gcttttacta ggtttgccga ttgctacgtt gacattgata ttgaaaaata tttacttagg
     5041 gcttttgatt ccaattatga accataaagc agacaattat gcagaagaag gaaattataa
     5101 aggagtcgag catatgcact tgcttgccgg tatcggtctt tccttgatgc ttggtgttgt
     5161 ggtaatggtt tcttatatgg tgggaagcaa tacgatcgga aatctattaa atatgattcc
     5221 tgacttcgtt caaaaggggt tgtcagtagc tacagggttg attccagctc ttggttttgc
     5281 gatgcttgct cgtttgctga tcaacaaaca agttgcacca tattttttct taggttttgc
     5341 tatagcttct tacttagaga ttcctgtgac cggagtagct atttttggtg cgattttagc
     5401 agttgtggta gtcaatatta tgaatgtccg tcaactacca cttcaaacaa ctgcagggga
     5461 ggtagaagaa gatgaagaat tctgagatca aaaacagcaa aataacgaaa aaagagctaa
     5521 atcacgtatt ttggcgttct tttcaaatgg agttttcttg gaattatgaa cgtcagatga
     5581 atatggctta tgtttatgca atgattccga ttttgaaaaa gttatatcag tcgaaagaag
     5641 aaatggcagc agctttgaaa cggcatctag agttttttaa cacgacccca catatcgtta
     5701 cactgatttt aggaatcacg gctgcaatgg aggaagaaaa taaggatgat cctgaatttg
     5761 atgtgactgc gatcgatagt atcaaaacat ctttgatggg acctcttgca gggttaggag
     5821 attctttctt ttggggaact ttgcgattga ttgccacagg tgtgggtacc tctttagcca
     5881 tgcaaggaaa tattttaggc cctatcttgt tcattttgat tttcaatatt ccgcacattc
     5941 tgtttcgcta tctagcaaca ggttggggat atcgcttagg aacgggtttt ttgaaaaaga
     6001 tccaggcaaa cggtatgatg gaaagtttga cattaggcgc gtctattatt ggactgatgg
     6061 tcgttggagc aatgactgcc acgatgatca ccatcaatat tcctttaaaa atcggttcag
     6121 gagaaaatgc tgtgactgtt caaagtattt ttgatgatat cgttccaaat atcttatgtc
     6181 ttggagcatt tggcatagtg ttttatctat tgaagaaaga agtgaaacca ttaacgatct
     6241 tgatcggact agctttcttt ggaatttttg gttcgtggat agggattttt taaaaaagga
     6301 gcagagaaaa tgtttacgat gcaagattat atctatgaag aaaaggaagt actaagtacg
     6361 atcttgaaaa agaatgattt ttcgacaaga gaacatatga aaaaaacagt gaatttactg
     6421 atcttagcaa cgggttcatc ttataacgct tgtttagcag cgaagccagc attggaatct
     6481 tatggtgatc ttactgtcga tattcaagaa cctttttatt ttttcaatta tggaaaactt
     6541 tctccttcaa tcgatactgt gattgctgtg tcacaaagcg ggaaaagtgc ttctactgtt
     6601 gaagcagtaa aaatgataca aaaacaaggg ttacctgttg ttgctatcac aaatgacgtg
     6661 caaagtccac ttgctttaga agctgcacaa attattgatt taggtgccgg ggtagaatca
     6721 gtaggcttcg ttacaaaagg ctattctgcg actgttttac agttactact cttagggata
     6781 ggaattggaa taagtaaaaa taaaatcagc aaaaaaatag aacaagacta catgcaacag
     6841 ttgcgcaaaa taatcaacca tttaccagcg attattcaaa aaacggaaga attttttgat
     6901 ggatatcaaa gtttatttcg tttagcacag cgtttcgtca ccattggata tggtccaaac
     6961 tggggaacag ctaaagaagc agagacgaaa ttgactgaaa caatccgcgt tccgtcacaa
     7021 ggatttgagt tagaagctta tatgcacggc ccatacctgg aggcagatgc ttcgcatttg
     7081 ctattcttta ttgaagggga aagtgtaaat aaggaacgtt cgcaaaagtt gcaaagatat
     7141 atgagtcggt acgttggaga gactctcacc attaccacaa aaaaagcaag aaacgagaaa
     7201 acacttggat tagcaataga atgtgatgaa tatctgtctg tattagcgct tgtcgttccg
     7261 tttcaactat tcgcttataa aaccgcagta gctaaaggta ttgacttgaa caaaaagatt
     7321 tttgaagact ttgatacagt cttgaaaagt aaattataag aggtggaaat catgctgaaa
     7381 ttcaatgaag aagaacaaat caaagcaata aaaggcgcac tagctttacg tccgcaagta
     7441 gaagaaatca tcgataaact ttatgcggaa aaatttgacg ctgtctatta tctaggaatc
     7501 ggtggaacgt atgcttcctc gatgcaagct gtcacttata tgaatgggaa aagcaacttg
     7561 cctgtctttg tacagcatgc tgcagaatat tacactactg gaaacaaaag attaacaaaa
     7621 gattctatcg ttattttgtc ctctgttacc ggtacaactc aagaagtagt gaaagcagta
     7681 gaacaaatca agaaagtagg agcgacactg attggattta tcgataaagc gaacagtaag
     7741 ttgtctcagc tatgcgattt tgtggtcact tatccagctc ctggaacaga acaaatcaaa
     7801 ttttttatgg cagcagatcg attgatgtat ttgaatggag aattcgaagc ttattcagaa
     7861 tactatgaac agctcgaacg ttatttacca acagggttag tggaagcaga gaaaaaagca
     7921 gatgcatttg gcttatcatt tgcagaaaaa catcgacgtg atagtatgca ttattttatt
     7981 ggagcaggga atcaatgggg agctgtttat tcatatgcca tgtgttactg ggaagaacaa
     8041 agctggcttc catcgaaatc tatccatgct gccgagtttc ttcacgggac gttagagatc
     8101 gtagaagaaa caaccccagt cacattattt ttaggcgaag atgaacagag agtgttatct
     8161 gaacgtgtgg ctaaactttt gccaaaaatt tgcagcaatt atacattgat agacacaaaa
     8221 gactatccag tagaaggtat cagtgaaaaa tatcgcggac gtgttttgtc tttcttattg
     8281 atgcatgtag tgacacaacg catcgatgct catgtggagc agttgaactg ccatccatta
     8341 gagatacgcc gctactatcg tcagtttgat tattaggata gactggataa aaagaatata
     8401 aaaaataaag agagcagcga taactgctcc ctttattttt ttaaaatatt ggcatcaatt
     8461 acgttataaa acacattttt tgcattatct acagttcaac ttataatctg tttaatggga
     8521 catgtgggtg ccaaccattt tttgttatgc aatagtacta tttagttgtt gcatggatcc
     8581 gtagtaaaag tccaagtaca acgagttgcg ccagtttaca tagcgttttg tatccagaat
     8641 gttgactaaa acaaaagttc ctatgtcaac cgtcaacaga gacaatctgt ccgttgcagg
     8701 acctgacaga tttagaaatg ttcaatctgt ctcttatgta ttttaaatca atactcacta
     8761 ggagaagttt cctaacctcc taatagtaat gctggtagta acattgtcaa aactgcttcc
     8821 tcttaggaac aagcaaagca taacgagtgt aaaaataaaa tgtagactat tgtaacatat
     8881 cattgattat ttcaagtaaa aacgataaaa gaagtaaaca aacacttaaa aacaatggaa
     8941 attagtccca actttaagaa atgtatacta taatggaaat tatcaattat ttatttggag
     9001 gaatatccat gaaacaaaaa atcgcagttc ttggtcctgg atcttggggc actgctttag
     9061 cacaaacatt ggcagaaaac gggcatgatg tccgtatttg gggaaatgta cctgaacaga
     9121 ttgatgaaat caataagtat catacaaatc agcatttttt acctgatttg acgatcccag
     9181 aatcgattat cggatataaa gaattggcag aagcagtgga taacgcagat gctattttat
     9241 ttgttgttcc aacaaaagcg attcgttcag tcgcacagga acttattggt aaaatgaata
     9301 ctaagccaat cattatacat gcaagtaaag gactagaaca agatacgcat aaacgaatct
     9361 cagaagtttt ggctgaagaa attccagaag aaaaaagaca ggcaattgtt gtattatctg
     9421 gtcctagcca tgctgaggaa gtggcagtac atgatatcac aacaattaca gctgcaagta
     9481 ttgatcaaaa agcagcagcc tatgtacaaa aactgttcat gaatgattat ttcaggattt
     9541 atacaaataa tgatgtgatt ggcgtagaaa ctggtgcagc actgaaaaat atcattgcaa
     9601 ttggtgccgg cgctatccat ggattaggat tcggagatga tgcaaaagca gcaatcatga
     9661 ctcgaggatt ggcagaaatc agccgtcttg gtgtcgcgat gggtgccaat cctctgacat
     9721 ttatcggttt gagtggtgtg ggggacttga tcgttacttg tacaagtgtt cattctcgca
     9781 attggcgtgc cggtaacttg ttaggacagg gacataagct ggaagaagtt ctagaaaaca
     9841 tgggaatggt cgttgagggt gtggcaacga caaaagcagc agtagagcta gctcaacagc
     9901 taggtgttga gatgccaatt actcaaacaa tctataatgt tttgtataat ggagaagata
     9961 tcaaaaaagc agcaaaagaa atcatgttac gtgacggaaa aatggagaat gaattttctc
    10021 ttcgttaaat agatgatcaa tagttaatcg t
//
LOCUS       DARBEC010000099         9473 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.103, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000099 DARBEC010000000
VERSION     DARBEC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.103"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(225..611)
                     /locus_tag="SCL80_002396"
     CDS             complement(225..611)
                     /locus_tag="SCL80_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEG1108237.1"
                     /translation="MNKRKTEFILILISGITGILCSLLALMGGMFLSSIDSTTLNTTE
                     LTSKDIDMLVNLSGNVVLFGGITLVFSIMIFIIGFLVRKNQKTVVYGISLLILSIIPF
                     FLLTLLWVIPGVLGIIAGLMLLIRKS"
     gene            complement(608..1129)
                     /locus_tag="SCL80_002397"
     CDS             complement(608..1129)
                     /locus_tag="SCL80_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEG1108238.1"
                     /translation="MNKKSGIETLPNYVFAGFWLRFFAFLIDLICISIITHATIGLFY
                     KIMGLESDKSIITFYGIASLAIYLTYFVLLTKLNSGQTIGKMIFGIRVVSFEEKELSW
                     QTIFIRECICRFILKNFVFQIGYLVAAFTPKKQHIGDYFSNTSVVVIRSLEAINYGQK
                     ISNSKTLNLERIK"
     gene            complement(1131..2165)
                     /gene="sppA"
                     /locus_tag="SCL80_002398"
     CDS             complement(1131..2165)
                     /gene="sppA"
                     /locus_tag="SCL80_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEG1108239.1"
                     /translation="MNKRRWIAVGVACGLLLLSIIVALIPGKDKEEASNTTLTGINKI
                     FYGSNEITEETLEEGASNKKIVKLSVNGVIADTGESNLFSREQYNHQNFLTQLKKIQE
                     DKAVKGVLLEVNSPGGGIYESAEIAKEMAKIKKLDIPIYTAFKNTAASGGYYISAGSD
                     KIFATEETTTGSIGVIISGLNYSGLLEKLGVTDATYKSGALKDMMSPQHKPSEEENKV
                     IQEFVMSAYDRFVNVVAKGRNMDTNAVKELADGRIYDGNQAVENGLVDQIGYSEDALD
                     SLKKEKKLTDATVIEYKNDTTGFASSWLGNKIAEWQGLKATTSDRVISVFEKLGFSDT
                     PKPMYYYGGL"
     gene            complement(2198..3613)
                     /locus_tag="SCL80_002399"
     CDS             complement(2198..3613)
                     /locus_tag="SCL80_002399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HEG1108240.1"
                     /translation="MVSIKNYGKSRRGKQFMQNNNWFEHSSIYTKQHHSFYRWVLYPV
                     ILFLCIIGLFLTFAKKEVVIRTTAQLNPEKIEKLQVPLEAKIMENFLKENQFVHKDDV
                     LVRLDCSLIENEKAQIEQENQRITQQIKMAQLFIESISKGKNLFSTDDSFGYSNQLKS
                     MLSEKESLRYALKQSELNDQKQLEVYEKTKRQLEKQIESSDSKLQEWQQVQVAWSNNQ
                     SLKDFSKEMMANYENWQEQLNNVSDDQKNQVKLTISASINEQIEQLKKEVEQYQSEKA
                     KLVKPTTSENDRISQTEKGKQELEQAITTVKQTVTELNEKQEKNQSVIKSLDDQLSKG
                     ILKAPVSGTVHLNEETKGQAELAKGTVLAEIYPIHEKSKMKFTALLPANESTYIKEGM
                     KVHFKLDQKGGAPITIDGTLDEISENSTATEKGVFYAVKGTLKTTQSFPFRYGLTGEL
                     SLIVGEKTYWQQIVDVFLNKK"
     gene            complement(3582..5735)
                     /locus_tag="SCL80_002400"
     CDS             complement(3582..5735)
                     /locus_tag="SCL80_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide cleavage/export ABC transporter"
                     /protein_id="HEG1108241.1"
                     /translation="MIKSFPFVQQQDEKDCGVACLSMILKYFKTDIPIHKLRDLSGTD
                     LEGTSAFGLKKTFEKLSFDCMAIQADSEIWKERDLILPLIAHILVDNSYMHYVVIYKT
                     KGNVLWIADPANGKNRKTIEAFQKEWTGILLLPMPRETYIPIKEKVAGLSSFLPIIWK
                     QKGLVFHIVLASIFITFFGIGSSYYFQGILDFFIPNQARSTLNIISIGLIAVYLFRVL
                     FEYSRSYLLIILGQRMSMAIMLQYFKHVLTLPMNFFATRKSGEIISRFLDASKIIDAL
                     ASATLSIFLDIGMVLLVGVTLAIQNSTLFLITLCSLPFYVVAIFAFVKSYEKSNREEM
                     AAGAAVNSSIIESLKGIETIKAYSGEEKVYNRIDHEFVKLMKKSFRTVTLDNIQQGIK
                     HLIQLISSALILWLGSYFVMDNRISLGQLITYNALLVFLTDPLQNIINLQVKMQTAQV
                     ANNRLNEIFAIDSEKSNQSFEYMLSEQLFQQDITVKNVSFSYSMKAPTLNEISCTIPS
                     HSKTAIVGVSGSGKSTLAKLFVRFYEASEGSIHYGNINHLDIPFQILRDRVTYLPQES
                     FFFSGTIIENLIFGLDSPPSFEQIIEVCEIVQLNEFINQQPLRYETLLEEGGNNLSGG
                     QRQRLALARALLKNPDILILDEATSGLDTLLEHAIMNYLLNLKEKTIIFIAHHLPIAK
                     ACDQILVLHEGHLVEKGTHDELRFADGIYQKLWEI"
     gene            5993..6229
                     /locus_tag="SCL80_002401"
     CDS             5993..6229
                     /locus_tag="SCL80_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin"
                     /protein_id="HEG1108242.1"
                     /translation="MFKHAKCKGTKYCGNAQTVGGGNNAWGKLGQVVGGLTTGAVGGA
                     GLGTAICGPACGVVGGLYGAVAGGAAAGWDARKK"
     gene            6248..6463
                     /locus_tag="SCL80_002402"
     CDS             6248..6463
                     /locus_tag="SCL80_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Blp family class II bacteriocin"
                     /protein_id="HEG1108243.1"
                     /translation="MHIKNTKTTFILSSEELKNIQGGSAVGVLGTTFSGATAGVKLCS
                     AGGPYAIAACGVGGALLGAGFSMWTGA"
     gene            complement(7166..7918)
                     /locus_tag="SCL80_002403"
     CDS             complement(7166..7918)
                     /locus_tag="SCL80_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108244.1"
                     /translation="MSYPIILCEDQIIQLNQLERIIDNFILFHDKVFKIVLKTQSPLE
                     VKKYLKQFRPKQGIYFLDIDLNHEVNGIELAEVIRKYDVQAKIIFTTTHDEMLPVTIK
                     RRVETLGFVTKDQTLDEYRNEIVELLLLAQERIDATKQVTNQAFVFSIGSQTFTFDIN
                     DIYFVEASKLPHRLSLCTKDGQYEFYGRISELEKKYPMLTRISRACLVNIFNVKEIDF
                     KKRSLYFDSELARNFTLGKAQKIKEKLKESTV"
     gene            complement(7933..9180)
                     /locus_tag="SCL80_002404"
     CDS             complement(7933..9180)
                     /locus_tag="SCL80_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEG1108245.1"
                     /translation="MFVGNFFFFLLFYMTNTDLNKRKYLFVCLLIPLTFFVELFTDVS
                     AAIPIIGSYFILKEKKKTNIVLLNKLLLCMLIDDFRSIITSMVMTYMFSFEGIKGYGY
                     VVFQIMLDSLFLLMFIWLYRKLAINRIVEQSSSKLTAIFMIYLLTIGLFVSYVAHQYQ
                     VFDHFVFGVLIFLIIQMFVVLFLFIRITIRQREQYEQQLKRKELVYLKKYTDSLEKDQ
                     EKFARFRHDYKNLLLSLKEIANQNHDSILEKEIKELEIYSNSYLTNKFEYRYLKNIKN
                     EYLKSLLIAKLYQANNNKICCKFECPSTIIEVPVPIFDCIRVLGIILDNAIEASIESQ
                     DRELSLAIYRDDKQIEISISNSCREILLSIDTLMKKGITTKKGHQGLGLSSIEEINKK
                     NPNMFVNYQNDPYQFTTQVILLF"
     gene            complement(9256..9402)
                     /locus_tag="SCL80_002405"
     CDS             complement(9256..9402)
                     /locus_tag="SCL80_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEG1108246.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFSLF
                     KKCN"
BASE COUNT         3102 a         1673 c         1320 g         3378 t
ORIGIN      
        1 aaaagtgaag aatatttaat agaaaaacta aatttacttg ctgctttccc cttttccatt
       61 aatacaacaa tcagagagtg tctttttttc gaaaaaatta gaataaataa aagacacgta
      121 gtttttttat ctacatgtct tccatcgact acaagttatc atttctcaaa acgtatttag
      181 cttttaattg attaatcgtt tcacgttgtc tacacattgc ctaattaaga ttttcttatt
      241 agaagcatca atcccgcaat aatacctaaa actccaggaa ttacccacaa taatgttaaa
      301 agaaaaaatg gaataataga taaaatgagt aaactaatac catagaccac tgttttttga
      361 ttttttctta ctaaaaaacc tattatgaaa atcattatcg aaaaaactag ggtaattcct
      421 ccaaataaaa caacattccc gcttagattg actaacatat ctatatcctt actggttagt
      481 tctgtagtat ttaaagtagt tgaatcaata gaagatagaa acataccacc catcaatgct
      541 aaaagactac ataaaatccc tgttattcca ctaattaaaa tcaaaataaa ctctgttttt
      601 ctcttattca tttaattctc tccagattaa gtgttttaga attagaaatt ttttgaccat
      661 agttaatagc ttctaatgaa cgaataacaa ccactgacgt gttagaaaaa taatccccaa
      721 tatgttgttt cttgggcgtg aaagcagcca ccaaataccc aatttgaaat acaaaatttt
      781 ttaatataaa cctacatata cactcacgaa taaagatagt ttgccaactt agctcttttt
      841 cctcaaagct cactactctt atcccaaaaa tcattttccc aattgtttgt cctgaattca
      901 gcttagtcaa aagtacaaaa taggttaaat aaatagctaa tgacgctata ccataaaaag
      961 taataatgga tttgtcagat tccagtccca tgattttgta aaagagacct atagtagcat
     1021 gggtgatgat gctaatacaa attaaatcaa taagaaaagc aaaaaatcgt aaccaaaatc
     1081 ccgcaaatac ataattaggc aaagtttcaa tgccactctt cttattcatt ttatagccct
     1141 ccatagtaat acattggttt tggagtatca gaaaatccaa gtttttcaaa aactgaaatt
     1201 acacgatctg atgtagttgc ctttagccct tgccattcag caatcttatt acctaaccaa
     1261 gaagaagcaa agccagttgt gtcattttta tattctataa ccgtcgcatc agttaatttc
     1321 ttctcttttt ttagggaatc taatgcatct tcagagtaac caatttgatc caccaaccca
     1381 ttttctactg cttggttacc atcataaatt cttccatccg caagttcttt cactgcatta
     1441 gtatccatat ttcttccttt tgctacaaca ttgacaaaac gatcataagc actcatcaca
     1501 aattcctgta taactttatt ctcttcctct gaaggtttgt gttgaggtga catcatatct
     1561 tttaatgcac cacttttata cgtcgcatca gttacgccaa gtttttctaa taagccagaa
     1621 taatttaaac cagaaataat gacaccaatt gatccagttg ttgtttcttc cgtagcaaat
     1681 attttatctg aaccagcaga aatgtagtac cctccacttg ccgctgtatt tttaaatgca
     1741 gtatagatag gaatatctag ctttttaatt tttgccattt ctttagcaat ctcagcactt
     1801 tcatatattc ctcccccagg tgaatttact tctaataaca ctcccttaac cgctttgtct
     1861 tcttgaattt tctttagctg tgttaaaaaa ttctgatggt tatattgttc cctactaaaa
     1921 agattgcttt cacctgtatc tgcaataact ccattgacgg atagtttgac aatcttttta
     1981 tttgatgcgc cttcttctaa cgtttcttct gtgatttcgt tagatccgta aaaaatctta
     2041 tttattcctg ttagggttgt atttgatgct tcctctttat cttttcctgg aattagcgcg
     2101 acgataatgg atagtaacaa taatccgcac gctactccca cagcaatcca tcttctttta
     2161 ttcatataaa ctcctcctat attcttagta aaaataatta ttttttattt agaaacacat
     2221 cgacaatttg ttgccaatat gttttttctc ctacaattaa agacaactct ccagttaatc
     2281 cataacgaaa cggaaacgat tgagttgttt ttaacgttcc tttcactgca taaaaaacac
     2341 ctttctcagt ggcagtagaa ttttctgaaa tttcatctaa agttccgtcg atcgtaatcg
     2401 gagccccacc tttttgatcc aatttaaaat gaaccttcat cccttcttta atataggtac
     2461 tttcattcgc aggtaaaagt gctgtaaatt tcatttttga tttttcgtgg atcggataaa
     2521 tttctgcaag cactgttcct ttggctagtt ctgcttgtcc ctttgtttct tcatttaaat
     2581 gaaccgttcc agatacagga gcttttagta ttccttttga aagttgatcg tccaaacttt
     2641 tgataactga ctggttcttc tcttgttttt catttagctc tgtaactgtt tgttttacag
     2701 ttgttattgc ttgttctagc tcttgctttc ctttttccgt ttgactaatt ctgtcattct
     2761 cagaagtagt tggtttaact aatttagctt tttctgactg atactgttct acttcttttt
     2821 ttagttgctc aatttgttca tttatgcttg ctgaaattgt cagtttcact tgattttttt
     2881 gatcatcaga aacattattt agttgttctt gccaattctc atagtttgcc atcatttctt
     2941 ttgaaaaatc ttttaatgat tgattattac tccaagctac ctgtacttgt tgccattctt
     3001 gtaatttact atctgaactc tcaatttgtt tttctagttg tctttttgtc ttttcgtata
     3061 cttctaattg cttttgatca tttaattcac tttgcttcaa agcgtagcgg agtgattctt
     3121 tttctgacaa catgctcttt aattgattac tgtagccaaa actgtcatcc gttgaaaaca
     3181 aattttttcc tttacttata ctttcaataa atagctgagc catctttatt tgttgagtaa
     3241 tacgttggtt ttcttgttct atctgagcct tttcattctc tattaaagaa caatccaatc
     3301 taaccaacac atcgtcctta tgaacaaatt gattttcttt taggaagttt tccattattt
     3361 tagcttctaa tggcacttgt aacttctcta ttttttcggg gttcaattga gccgttgtcc
     3421 gaataacaac ctctttttta gcaaaggtta aaaacaatcc aataatacat aaaaaaagaa
     3481 ttacagggta taacacccaa cggtagaagc tatgatgttg tttagtatag atactagaat
     3541 gttcgaacca attattattc tgcataaatt gtttccctct cctagatttc ccataatttt
     3601 tgatagatac catcggcaaa acgaagttca tcgtgcgttc ctttttctac caaatgccct
     3661 tcatggagaa ctaaaatttg atcacatgct tttgctattg gtaaatgatg cgcaataaaa
     3721 ataatcgttt tttcttttaa attgaggaga taattcataa ttgcatgttc taacaaggta
     3781 tctaatccac tagttgcttc atctaaaatt aatatgtccg gattttttaa taaagcccgt
     3841 gctaaggcta atcgttgtcg ttgccctcct gaaagattat ttccaccttc ttccaacaat
     3901 gtctcgtaac gtaatggctg ttgattaata aattcattta gttggactat ctcacaaact
     3961 tctattattt gttcgaaact tggtgggcta tctaaaccga atataagatt ctcaatgatt
     4021 gttccactaa agaaaaatga ctcttgaggc aaatatgtca ctcgatcgcg taatatctga
     4081 aaaggaatgt ctaagtgatt aatgtttcca taatggatcg atccttctga tgcttcataa
     4141 aaacgaacaa ataactttgc aagagttgat tttccagaac cactaactcc aactatagcc
     4201 gttttactgt gcgaaggaat tgtacatgaa atttcgttca acgttggggc cttcatgcta
     4261 tacgagaatg aaacgttttt tacagtgata tcttgttgaa ataattgttc agaaagcata
     4321 tattcaaaag actggttcga tttttctgaa tcaatcgcga aaatttcatt taaacgatta
     4381 tttgcaactt gagccgtttg cattttcact tgtaaattaa taatgttttg taaaggatct
     4441 gtcaaaaata ctagtaaagc attgtatgtg ataagttgac ctagactaat tctattatcc
     4501 ataacaaaat aagaacctag ccataaaatt aatgcactgc taattaattg aatcaaatgt
     4561 ttaattcctt gttgaatatt atcaagcgta accgtacgaa atgatttttt cattaattta
     4621 acaaattcat ggtctatacg gttatatact ttctcttcac cgctatatgc tttaattgtt
     4681 tctattcctt taagactttc tatgatacta gaatttacag ctgcaccggc tgccatttct
     4741 tccctgttcg atttttcata gcttttcacg aaagcgaata ttgcaactac ataaaaaggc
     4801 aaagagcata gagtgattaa aaacaacgta ctattctgaa tagctaacgt aaccccaaca
     4861 agcaacacca taccaatatc taaaaatatt gataacgtag cacttgctaa agcatcaata
     4921 atcttactgg catcaagaaa acgagaaata atttcccctg attttctcgt agcaaaaaaa
     4981 ttcattggta aagttaacac atgtttgaaa tactgaagca taattgccat actcattcgc
     5041 tgtcctaaaa taataagaag ataactacga ctatattcaa acaatacccg aaataaatag
     5101 acagcgatca agccaatgga aattatattt aaagttgaac gtgcttgatt agggatgaaa
     5161 aaatcaagaa taccttgaaa ataatatgag cttcctatgc caaaaaacgt aatgaaaatc
     5221 gatgctaaaa cgatgtggaa tactaatcct ttttgtttcc aaattatagg caaaaaagaa
     5281 cttaatccag ctactttttc tttaatagga atataagttt ctctaggcat aggtaataaa
     5341 agaatacctg tccattcttt ttgaaacgct tcaatcgttt ttctattttt tccattagcg
     5401 ggatctgcaa tccatagtac gtttcccttc gttttataaa ttacaacgta gtgcatatag
     5461 ctgttatcaa ccaatatatg tgcaattaat ggtaagataa gatctctttc tttccagatc
     5521 tcactatctg cttgaatagc catacaatca aaagacaatt tttcaaatgt ctttttcaat
     5581 ccaaatgctg aagtaccctc taaatcagtc ccagataggt cccgtaattt atgaatcggg
     5641 atatcagttt taaaatattt taaaatcatc gaaaggcaag caacaccaca atctttttca
     5701 tcttgttgct gcacaaaagg aaatgatttt atcacttatc taacacaact ccttctaaaa
     5761 aatttttata gaaaaattct caatatagct ttaatacagt gaaaatattg tctcataatt
     5821 ccacaaaaac tttgaaaaca gttcttgaac actaactttt tcattcctga atcaaattta
     5881 ttttggaaat tttactattc aagaataaac ttgataagtt tcaggaagat ttttactgtt
     5941 aatcaaatac gtatgttaca gtagcaccgt aaaaaatatt atttaaagaa gcgtgtttaa
     6001 acatgcaaaa tgtaaaggaa ctaagtattg tggaaatgca caaactgttg gtggaggcaa
     6061 taatgcttgg ggaaaattgg ggcaagttgt aggtggactt accacagggg cagtaggtgg
     6121 tgcaggttta ggaacagcaa tttgcggacc agcttgcggt gttgttggag ggttatatgg
     6181 agctgtcgct ggaggtgctg cagctggctg ggatgctcga aaaaaataaa aaaggagaat
     6241 aagtagtatg catattaaaa acacaaaaac aacatttata ttatcttcag aagagttgaa
     6301 aaatattcaa ggtgggagtg ccgtgggagt tttaggcact actttttctg gggcaacagc
     6361 aggagtaaaa ctttgttcag ccggaggacc ttatgcaata gctgcttgtg gtgtaggtgg
     6421 agctttatta ggtgctggat tcagcatgtg gaccggtgcg taaacaatta aaaaatatca
     6481 aacctcaaga ttggtttgtg tcgattacga acataggtat gttctttatc ttttcaaaag
     6541 tttggaatgg acagaacttt ttactaaagc ttcttgtgct agtttttata attatattaa
     6601 atttttatct tttattaaag tcagataaaa attctttgga aatgtaatgg tagagatatt
     6661 tttacaaaag gacttgcagc gctgtagttg gtgctgcagg aggacctatt tcggccgtta
     6721 caggaggtat tggtaatgta cttgtcggat gtgcattaaa gcctgttcct gcaagttaaa
     6781 tttataaaaa caggaaagta attatctcaa atcctatcaa gtacaatcat aggattgcac
     6841 ttgacaggac aaatttttta actaaaaaat gaaaaacact ttctaaaaat tagtagaatt
     6901 tgccaaatct ttcgcatctt ccaccataca tttaatttta attccccttt atggtactta
     6961 ataaaaacaa gaaaagaact attcatttgg cccttttttg gttccagacc atagaaatat
     7021 tcctccagta actaatattg ctgttatgca cgtaactaat aacagctata aaaattggta
     7081 caactttaat aggattcaga aaaatttgca ctagtgaagg aatctatttt attagaaaaa
     7141 tgcaatctcc ctaaagaaaa aaccactaaa cagtgctttc tttcaacttc tccttaattt
     7201 tttgtgcttt ccctaaagta aagtttctgg ccaactctga atcaaaatat agtgaacgtt
     7261 ttttaaaatc tatttcttta acgttaaaga tatttactaa acacgctcga ctaattcttg
     7321 ttaacatagg atatttcttt tcaagctcac taattcttcc ataaaattcg tactgcccat
     7381 ctttagtaca caatgataat ctatgtggta attttgaagc ttctacaaaa taaatgtcat
     7441 ttatatcaaa agtaaatgtt tgagaaccta tggaaaaaac aaatgcttga ttcgtaacct
     7501 gttttgttgc atctatcctt tcttgcgcta ataacaataa ctcaacaatc tcgtttcgat
     7561 actcatctag tgtttgatct tttgttacaa atcctaacgt ttcaactctt ctttttattg
     7621 ttacgggtaa catctcatca tgagtagttg taaaaatgat ttttgcttga acatcatatt
     7681 ttctgattac ttctgctaat tctataccgt taacttcatg atttaaatca atatctaaga
     7741 aatatattcc ttgctttggt cggaattgtt tgaggtattt tttaacttct aacggacttt
     7801 gtgttttcaa tactatctta aatactttat cgtgaaaaag aatgaaatta tcaataattc
     7861 gttctaactg atttaattgt ataatttgat cttcacataa tattattgga taactcataa
     7921 atcctccttc cctcaaaaca acaaaataac ttgagtagta aattgatacg gatcgttttg
     7981 atagttcaca aacatattgg gattcttttt attgatttcc tctatactag ataaacctaa
     8041 cccttgatgt cccttttttg ttgttattcc ttttttcatt agagtatcta tagagagtag
     8101 tatctctcta caactattcg atatcgaaat ttctatctgt ttatcatctc ggtaaatagc
     8161 tagagacagt tctctatctt gactctctat agatgcttca attgcattat cgagaataat
     8221 tccaagcacc ctaatacagt caaatattgg aacaggaact tcaataatag tacttggaca
     8281 ttcgaattta caacaaatct tattgttatt tgcttggtat agcttcgcta tcaacaaact
     8341 tttcaaatat tcatttttta tattttttaa ataacgatat tcaaacttat tggttagata
     8401 agaattagaa tagatctcta attcttttat ttctttttcc aatatcgaat catggttttg
     8461 attagcgatt tcttttaaac tcagcaataa atttttatag tcatgtctaa atctggcaaa
     8521 tttttcttga tctttttcca gtgaatcagt atattttttt agataaacca attcttttct
     8581 tttcaattgc tgttcatatt gttctcgttg cctaatagtt attctaataa ataaaaatag
     8641 aacaacaaac atttgaatga ttaaaaatat taagacacca aacacaaaat gatcaaatac
     8701 ttgatattga tgagcaacat aagaaacaaa taaaccaatt gtaagtaaat aaatcatgaa
     8761 tatagcagta agtttagaac tggactgttc aacaatccta tttatagcta attttctata
     8821 taaccaaata aacatcagca aaaacaaaga atcaagcatt atctggaata ctacataacc
     8881 ataccctttt atcccttcaa atgaaaacat atacgtcata accatggaag taataataga
     8941 tctgaaatca tcaataagca tacataatag taatttattt aaaagcacta tattagtttt
     9001 cttcttttcc tttaatataa aataacttcc gattataggt atagctgcgg aaacatcagt
     9061 aaataattca acaaaaaaag tgagtggtat caataaacat acaaaaagat attttctttt
     9121 gttaagatca gtattagtca tataaaataa gagaaaaaag aaaaagtttc ccacgaacat
     9181 tcttaataga taagcttttg tatcgatagt aatcaagaga tcccctcctg attttaggat
     9241 atcataaaaa aaattttagt tacacttttt aaataatgaa aaaacacatt ccactaaatt
     9301 tgatgccggt tttccttgag gttttgttcc agcacctcct ttaatttttt taagctcttg
     9361 atctgaacat tgtttagcat ttagtctatt tttttcttcc attacgattc cccctacaaa
     9421 ctataaatac ttttatgtca ctgattcaat atacctattt ttctaatcaa aaa
//
LOCUS       DARBEC010000100         9361 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.104, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000100 DARBEC010000000
VERSION     DARBEC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9361)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9361)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9361
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.104"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(92..760)
                     /locus_tag="SCL80_002406"
     CDS             complement(92..760)
                     /locus_tag="SCL80_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1108247.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            894..1181
                     /locus_tag="SCL80_002407"
     CDS             894..1181
                     /locus_tag="SCL80_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108248.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            1597..3135
                     /locus_tag="SCL80_002408"
     CDS             1597..3135
                     /locus_tag="SCL80_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEG1108249.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            3132..4097
                     /locus_tag="SCL80_002409"
     CDS             3132..4097
                     /locus_tag="SCL80_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108250.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            4084..5406
                     /locus_tag="SCL80_002410"
     CDS             4084..5406
                     /locus_tag="SCL80_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEG1108251.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     regulatory      5469..5573
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            5647..6390
                     /locus_tag="SCL80_002411"
     CDS             5647..6390
                     /locus_tag="SCL80_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108252.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     gene            6405..7397
                     /locus_tag="SCL80_002412"
     CDS             6405..7397
                     /locus_tag="SCL80_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1108253.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            7409..8146
                     /locus_tag="SCL80_002413"
     CDS             7409..8146
                     /locus_tag="SCL80_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108254.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            8136..8858
                     /locus_tag="SCL80_002414"
     CDS             8136..8858
                     /locus_tag="SCL80_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEG1108255.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(8942..>9361)
                     /locus_tag="SCL80_002415"
     CDS             complement(8942..>9361)
                     /locus_tag="SCL80_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1108256.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQPVVQETVQTEK
                     QAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQLDSSYL
                     NGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
BASE COUNT         3068 a         1607 c         1972 g         2714 t
ORIGIN      
        1 tttttttata aagaagattt tactgaataa tatcatttta gtttacataa tgatatttct
       61 gtaaattaaa taaagtagtg aaaaataaga atcagtgctc taactgttca agaggatcaa
      121 gtggttcaaa tgtttctgta gtctgttttg cttccaattg gggcaaaaca gatccaattt
      181 ccagatactt cactttattc aatgtataag atagaataga acagctaagt aatgtataca
      241 gaaaatgtgt gagatccaaa taaagcaagg agacaactaa aataccaaaa gtaatcaaga
      301 aaagactttt gccaatctta atatgaaaat atttttcaat ggcttgataa atgatatctg
      361 ttccgcctgt gcttccgccg aatcgaaaga cgagaccgca accaatgcca gaaagaatac
      421 ctgtcatcaa actattgatc agtagactat ctacagcaaa atgtatatga aggctctccc
      481 ataaatgtaa aaagaaaact agcgcaaatg ttccgacgat cgtccggaga aaagcgtgtt
      541 ttccaaaaag aaacaatgag ataagcaata aaggtaaatt aaaaagcagt gaagtgacag
      601 aaggagcgaa tccaaaaaga ttatgcaaaa gcaaagcaat accagtaacc ccaccatctg
      661 ccagcttaga tggaatattg attcctgtca cagctaatcc gtagattcct gcgcctaaaa
      721 tgattccgca caattctgta aataaattta attttttcat ttctagcctc ttttcttttg
      781 aaaattttca gcaacttctt tattatactg acttatactc ttacttcaag aggaaaatga
      841 aaaaaaatta tttttggctg tttcttttta tttatcaaat aaggagtgga aaaatgcgag
      901 cagcagactt attgacaatt cttacaaaat cacccaaaaa aacagagtat caaggagttt
      961 ttttggctca agaacaaaaa ctggtgcctg tgacaatggt ccagataaat aaagcaaatc
     1021 aatttatctt tttatttgaa cacggcaaag caccacttgc tatgaaagac atctttcttt
     1081 ttttgatgaa gaaccgtgag aaagctgttt attatcagtc aaaagaagaa ctcattcctc
     1141 tttatggagt gaaagagata gaaaataaag tggtgatata agtaaaaata agaaaacaag
     1201 acgtttatga tgtcaaaaca accatttttt acatttttgg caaaaaagtg tcatgataca
     1261 gtataatatt cgttgaacag gccaataaag gtgttctccc ccacgagatt tctactttat
     1321 tggctgtttc tttttattaa aataaaagtt gtgtgtgtaa aaaagatata gctgattcca
     1381 agtgacaatt tcgtatagat ttttatggta ttcgtagtga aaaaacgaga accaaaaagt
     1441 tgatccgact ttgttgcttg gattcttttt ttgtcagcat atttagaggt atttttttct
     1501 tttttttgtt cgtgctgtaa ggttaaggca agacaagtta gttgggaaaa aggaaagaaa
     1561 tgtttatact taaagaataa cagaaaggaa ggtcgcatga ataattttat acaaacatta
     1621 agtgaaagaa aaggcgagtt gctggaagct gcatttcaac atctgtccat ttctctcatt
     1681 tctttgctga ttgcagcact gattgctatt cctttagcga tttgggcagc caatcataaa
     1741 aaaatggcag aagtcttatt gcagatcacg ggtgtgctcc aaacgattcc ttcacttgcc
     1801 ttattaggtt tacttatccc atttgtaggg atcggaactg ttccagcatt gattgcactt
     1861 gttatttacg ctttgcttcc gattttccaa aacacgtata ttggtctagc tgaaattgac
     1921 ccgtctattg aagaagcagc ggtagcattt ggtatgtcca gaatgagacg cttgctgaaa
     1981 gtagagcttc ccatcgcatt acctgtcatt atctcaggta tccgaactgc cttagtttta
     2041 atcatcggta cggctacttt ggcagcattg atcggagcag gtggattagg gacatttatt
     2101 ttgttaggta ttgaccgaaa tacacctgtt ttgacattga ttggtgcgat cagttctgcc
     2161 ttgcttgcaa ttatattcag cggactgatt cgctggttgc agcataagaa acctcgatat
     2221 tccatcatta ctttagcagc agtggtgtgt ttatttggcg gcgtttcttt atatcagtct
     2281 gccttgttta aaaatgaaac catcacgatt gctggaaaat taggagcgga acctgaaatc
     2341 ctgatccaaa tgtataaaga attgattgaa gacaacacaa aaacagaagt tgaattaaaa
     2401 ccgaattttg gcaagacgag ttttttgttc aatgcactgg atcaaggtca aatcgatatc
     2461 tatcctgagt tcacaggtac tgtcctagaa agcttggtga aggtacctga agcaatgaaa
     2521 agtgctgatt tgacaaaaga aaaaacatat gacgaagcag aagaattatt gaataagcaa
     2581 ttcgatatga cattattaaa accaatggct taccaaaata cgtatgcctt ggcagtcaag
     2641 agatcttttg cgaacgaaca tcaacttaaa aagatttccg atttggcagc tatctctgat
     2701 cagatcaaag cgggattcac actggaattt attgatcgtt cagatggata taaaggaatc
     2761 caagaagcat atggcttgaa tttttcaagt gtacaaagca tggagccaag tttgcgttac
     2821 caagcaatca ataatggaga tgtcaatatt gtggatgcct attccacaga tagccagctc
     2881 aaggagtacg atctagtgac tctagaagat gatcgagggt tattcccagc ttatcaaggc
     2941 gcaccgctga tgaaaaaaga ttttgctaaa gaacatcctg aaatcgttaa agcactgaat
     3001 aaattaagcg gaaaaataac ggaggatcaa atgattgata tgaattacca agtcaatgtc
     3061 gaaaagaaaa agccgacaga agtagcacga atcttcctga aaaatgaagg attagtaaag
     3121 gaggaagatc agtgactaaa ttaatcgagt tcaaacatgt gcaaaaaaag tatgatggaa
     3181 agtatgtcat cgatgaccta aatctggcaa tcaaaaaagg agaaatcttt gttttggttg
     3241 gtccttccgg tagtggaaaa acaactactt tgaaaatgat caatggatta tctaaaccat
     3301 cagcaggaga catctacttc aaaggaaaat cgttaaatga atataatctg cagaaaatgc
     3361 ggtggaatat gggatatgtg ctgcagcaaa tcgctttgtt cccgaccatg acagtcaagc
     3421 aaaatattga agtcatccct gaaatgttag gttgggaaaa gcagaaaaga gcagaccgtg
     3481 tagatgaact tcttcaaaaa gttggcttat ctcctgatat ttatcgagat cgtatgccta
     3541 gagagttgtc tggaggagaa cagcaacgga tcgggatcat ccgagctatt gctgcaagcc
     3601 cagatgtgat tttgatggac gagccattta gcgcattaga tccgatttca agaaactcat
     3661 tacaagaatt ggttttgtcc ttacacgaag aattgggcac gaccatcgtt ttcgttacgc
     3721 acaatatgga agaagcaatc aagctaggtg atcgtattgc atttatgaag gatggagaaa
     3781 ttatccaatg tgacacacca gagcagttat tgatgaatcc taaaaacgac tacgttcggc
     3841 atttctttga tgaaccgaaa cagacgaaag agtggcgagt agaagattta gtcgtcaatg
     3901 gatatttctt aaatgaaata ccagaaaatg ctcgtcaaca agtttgcttt gatacgccta
     3961 tgaaagaagt atattcgctt ctttcggtgt atccaagcat tgtgatagta gaaaagcaaa
     4021 gaagtattgg gtcgctgaca agtaaagaaa tctttgcttt tttaagtcga gaggaggaag
     4081 gaaatgaaaa catatgatgc aatcatcatc ggttcaggtg tgagcggctt aagtgctgct
     4141 tatggattga aagaagcagg taaaacagta ctagtggtcg aagaagatct atggggcggg
     4201 acttgtccta accgtggatg tgatcctaaa aaagtcttgt taagtgcagt agaggcacgt
     4261 aatcgggtaa aacaattgag tggaaaaggt tttaacgaga tcccaactgc caactgggaa
     4321 gaacttcaaa aattcaaacg aacatttacg gatccagtcc cagaaagccg aaaaaaacaa
     4381 ttagcagaag ctgaaatcga tcatctttca ggaaccgctc gttttttgga tgattcatct
     4441 attgaagtca acgaagaagt cttccatgcg gattacttag ttttggcaac cgggcaaaga
     4501 cctacgatct taccagtgga gggaaaggaa tacttgaaaa ctagtgcaga ctttttatca
     4561 ttgcctgtac tgcctaaaga aatcattttt atcggtggtg gttatattgc ttttgagcta
     4621 gctacgattg ccaatgctgc agggagcaaa gtaaccattg tccatcataa tcaacggcca
     4681 ttaaaagaat ttgaggcgtc tttggtagag gaagccgtcc atcaaatgga agcatctggc
     4741 attcagtttg cttttggtgt tgaaacccaa aagatcattt cagaaggtac tcgctatcgt
     4801 ttagtcggaa aagagacgga attagtagca gatatgattt tttgtgcgac tgggagacag
     4861 ccgaatacag aatctttagc acttgagcaa gcgaatatcg tctttgataa acacggaatt
     4921 gcagtcaatg attatctgca aacgagcaat ccaaaaattt ttgcgtgtgg agatattgtt
     4981 tcaaggaaaa ccccaaaatt gacaccagtc gccacttttg aaggaaatta tgtagctaag
     5041 cggataacgg atgcaacaag cgaacctatc aaatatccga ttatacctac gatcgtctac
     5101 gccagtccta aacttgctga agtaggagtt acaaaaagtc acgcctcttc gtcagatcaa
     5161 gtggtagaga tggatcttac cagttggttc acctatcatc gtgtcaatga accagttgct
     5221 aaggctgaat tgacctttga ccagcaaaat tatttgattg gtgctgcagt gattagcgag
     5281 caagcagacg aattgatcga tgatttgaca ctcgtcatca atcaaaaatt gactaaaaaa
     5341 gagttggata gctatatcat ggggtatccg acattggcaa gtgatctctc ctatttattg
     5401 aaataatcag catgataact aaagcgcttg ttgctttttc cttcttgtgc tacactttaa
     5461 aaaatgaata cctgtttctg gggatgtcgt agacgacatg agagtaagtt gtgaacttag
     5521 acccattgaa cctgtcagtt aatactggtg aagggagaaa catagcagac gcttgaagcg
     5581 ggttactatg tcgtgcccat ttcaagcgtt ttttgttatt catattagaa gaaaggaggt
     5641 aacaaaatga agaaaaatct ttatccagca gtcagtatgg cattcatttt aattatttgg
     5701 cagattgttg tcatagtttg gaaaatacct gcctttattt tacccggacc tgctgctgta
     5761 ttttctgcat tagcggcaga tgcacaagta ttactttccc attctgtcac gacattaaat
     5821 gaagcagtgc ttggtttggt aatagctagc gttctttctt ttattactgc cttggcaatg
     5881 gattattgga agtttttaga aatgtctttt tatccgttac tagtgatttc acaaatgttg
     5941 ccaatcatgg tgttagggcc attacttacc ctatggtttg gtttcggtat gcttccaaaa
     6001 gtgatccttg ttgtcttgat aagctatttt ccgatcgtag tttctttttc ggatacattg
     6061 aggaaagtat caaaagagca aatcatcttt ttgaaaacga tgggggcaga tacagggaag
     6121 atttatcgaa tatataaaat accagcgggt ttatccggct tcttttccgg tttaaaagtg
     6181 gctgctactt attgtgtcag cggagcagtc gttggagaat ggctaagcgc tcaagcaggc
     6241 ttagggtatt acatgatacg cgtaaaaaac agctaccaga tcgataaagt ttttgctgcg
     6301 atcatttgtg tcattctatt gagtttattg ctgaatggcg cttgttcatt attgaaaaaa
     6361 gggtattatc gtatgcttta tattttttaa gagaagggga tcaaatgaaa aaattatggc
     6421 ttttattacc gctactgttg cttttatccg cttgtggcac tgcaaaagaa acaagtagca
     6481 aacaagaaat aaaggatttg aaaaaagtga cacttgtttt ggattatgta ccgaatacta
     6541 atcatactgg catctaccta gcaaaagaaa aaggctatta taaagaggct ggtttaaatg
     6601 tccaaatcat cgaaccagga gataattcga caagtattgg tctagtaggt gcggacaaag
     6661 cacaatttgg tgtaagttat caagaagatg tcacttatgc tcacgcagat ggacagaata
     6721 taccagtcaa agcaattgct acagtgatca aacataatac ttctggattc gccaccctat
     6781 cagacagtaa catccattca ccaaaagatt ttgaaggaaa aacatatgca ggttggcaaa
     6841 gtccaagtga agaagctgtc ttaaaagctg tgatggaaaa agacggcggg gacttttcaa
     6901 aactgacaat ggtaggaagc aatggagaag ggccagaaag cttaggaaaa tcctcagata
     6961 tccaatggta ctttgaaggt tgggatatga tcaaagcaaa agaagcgggt attgaactaa
     7021 actatatccc attgaaagaa ttagatgaac ggttagatta ctatactccg gtaattatta
     7081 cgaatgatca actgatcaaa agtgatccag aattggtaca gtcttttatg gatgccacga
     7141 aaaaaggcta tcaggaagct attaaggatc caaatgacag tgctaaactg ttgcaaaaat
     7201 atgcaaaaga aaatgatcgg acgtttttag aagaatcaca agcattcttg tcaaaaaatt
     7261 atacggatga tcctgagaat tggggattga tggaagaaaa agtttggcgc aattatacag
     7321 cttttatgca ggaaaacgga ttgatcaagc aagatgtacc ttcgcaagaa ctattcacta
     7381 atcagtttat aaaataggag tgatagatat gtcaatccaa atcagccact tgaatgtagt
     7441 cattgaaaac ctgaaaatat tagaagacat ccatttcact atccctaaag atacctttac
     7501 atcgattgtc gcgccaagcg gagccggtaa gtcaacattg ctgaaaactt taacaggagt
     7561 ccagcctatg acttctggaa cgatcaaagt agacgatcaa aaagtaactg gtctccatac
     7621 agcatttagt tatatgccgc aagaagatat gttacttcct tggctgagcg tctatcaaaa
     7681 cgttacgtta tatcaaaaaa tcaatcatct aacaatcaat gaagaacaag taagggatta
     7741 tctggatatc tttgggttaa acggctatga acattttttg ccggaacaat tatctggcgg
     7801 gatgaagcag cgaacggcct tgttacgaac aatcatgaat ccttcttctt acttgcttct
     7861 tgatgaacca tttggagcgt tagatgctat gaccagaggg cagatgcaag actggatgtt
     7921 gaagctgcct aaaaaagcaa agcggactac tttacttgtg acgcatgata tcgaagaagc
     7981 aatttattta tcagacagga ttcttgtgct ttccgcccgt ccggcacatg tgattgctga
     8041 gattcaagtg cctgaaaaac gacggtcacg tgaatggcta ctgcagcaat cagagttgaa
     8101 gcaaacgatt tatcagttat tggcaggtga attagatgtt aagtgatgct cattgtcatt
     8161 tagatgggag tcagagttta gcactccttc agcaggaaca cacgattttg acgatcatca
     8221 attgtgattc gccagaagaa tggaaggaaa acagacagct tgcagcaagt aagactcaag
     8281 ccttaagtta cggtatccat ccttggaaag cagatagcta tacgtttgaa caagttgagc
     8341 cttttttgaa aaaagcaagg atcatcggtg aaatagggtt agataatatt tggacgaatg
     8401 taccgatgac tacacaaaaa aaagtatttg agcgacagct tgcttttgca gccataaatg
     8461 agaaacctgt tgtactgcac acaaaaggat gcgaaaaaga gatcttaaat tatatccaag
     8521 cttaccctaa tcactatttg atccattggt attcttcttt ggactatcaa caagactata
     8581 ttgatttagg ctgttatttc agcattggtc ttgatttgaa aaagaatcca gcagtctggc
     8641 aactagctaa agcagtccca atcgaccact tacttattga aacggatgga ctacatgcag
     8701 cagagtgggc tttagggggg attttttcag aaaaagatta tgtctcactg cttgaagaac
     8761 agttaaagac aatcgctgag atcaaagagc tatcagttga agaagttaaa aaaaatactt
     8821 atcaaaactt ggaacgtttc attagattag gtaaataaaa aagagatgtg acacaactag
     8881 tgtcgcacct ctttttggct ttctttgtat aaaagtaaac tgtttgatta ataaatgaat
     8941 cttagtacca accgtttgct tcccagaatg ctttcgccgc ttcccaagaa ccataacgtg
     9001 aagtaacata ttgttctgct actctttctt ggtttgcagc agagtagtca ccatttaagt
     9061 aagaagaatc taattggtaa cgtccgatgt aacgtccgtt cgttgctgtg taagaaccgc
     9121 ttgattcttt ttgtgcgatc cattcttttg cagagcttgt gcttgctgga gtagcagcag
     9181 tttctgttac agcaggagca ggttgtgttt cagcaacagg cgctgcttgt ttttccgttt
     9241 gaaccgtttc ttgtacaaca ggttgttcag cttggactgg tgcttcttga acggctgctt
     9301 gaggttcagc agctgcttgt gcagagcctt ctgttgggat tgttaattgt tgtcctgagt
     9361 a
//
LOCUS       DARBEC010000101         9281 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.105, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000101 DARBEC010000000
VERSION     DARBEC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9281
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.105"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..435
                     /locus_tag="SCL80_002416"
     CDS             <1..435
                     /locus_tag="SCL80_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M23"
                     /protein_id="HEG1108257.1"
                     /translation="YSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPVQAEQPVVQET
                     VQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYIGRYQL
                     DSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(759..842)
                     /locus_tag="SCL80_002417"
     tRNA            complement(759..842)
                     /locus_tag="SCL80_002417"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(806..808),aa:Leu,seq:cag)
     gene            1370..1870
                     /locus_tag="SCL80_002418"
     CDS             1370..1870
                     /locus_tag="SCL80_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108258.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            complement(2060..2404)
                     /locus_tag="SCL80_002419"
     CDS             complement(2060..2404)
                     /locus_tag="SCL80_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEG1108259.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(2558..3478)
                     /locus_tag="SCL80_002420"
     CDS             complement(2558..3478)
                     /locus_tag="SCL80_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEG1108260.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            3665..4156
                     /locus_tag="SCL80_002421"
     CDS             3665..4156
                     /locus_tag="SCL80_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEG1108261.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            complement(4180..6555)
                     /locus_tag="SCL80_002422"
     CDS             complement(4180..6555)
                     /locus_tag="SCL80_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEG1108262.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            6881..7507
                     /locus_tag="SCL80_002423"
     CDS             6881..7507
                     /locus_tag="SCL80_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEG1108263.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            7530..8234
                     /locus_tag="SCL80_002424"
     CDS             7530..8234
                     /locus_tag="SCL80_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1108264.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            8426..9259
                     /locus_tag="SCL80_002425"
     CDS             8426..9259
                     /locus_tag="SCL80_002425"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEG1108265.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
BASE COUNT         2828 a         1791 c         1651 g         3011 t
ORIGIN      
        1 tactcaggac aacaattaac aatcccaaca gaaggctctg cacaagcagc tgctgaacct
       61 caagcagccg ttcaagaagc accagtccaa gctgaacctg ttcaagctga acaacctgtt
      121 gtacaagaaa cggttcaaac ggaaacacaa gcagcgcctg ttgctgaaac acaacctgct
      181 cctgctgtaa cagaaactgc tgctactcca gcaagtacaa gctctgcaaa agaatggatc
      241 gcacaaaaag aatcaagcgg ttcttacaca gcaacgaacg gacgttacat cggccgttac
      301 caattagatt cttcttactt aaatggtgac tactctgctg caaaccaaga aagagtagca
      361 gaacaatatg ttacttcacg ttatggttct tgggaagcgg cgaaagcatt ctgggaagca
      421 aacggttggt actaagattc attatttgaa cgtttaaaca cattcaaaat atagtaagag
      481 gtcatgcgat ttattctttc gcatgacctc tttttgagtt taagtttagt ctttattcct
      541 taactatcgt ttgccagtat ttcttgtatc agaatctata aatctactat ttgaatcaat
      601 ctattaggtt ttaaactgtc ccaccttcac acaaattttt tttgatttat ttgaatctgg
      661 ttccgttgtt taaaattttt cctatattct tttagataat atgttatttt ctttctcatc
      721 gctccaggtg taccttctct ttataaaaaa acaaattatg cggctaagaa gactcgaact
      781 tccacgaggt caccctcact agcgcctgaa gctagcgcgt ctgccaattc cgccatagcc
      841 gcatcacatc ttttagttta ggattacatg tgttttttgt caagaacata tttttgttct
      901 ttttcatttc tgtacgccct cccttatgcc atctacacaa ataattcatc cgagcaactc
      961 catcatctat tgagggttgg tgaaaaaatc tgttttatcc atatgatcga tttcaatgtt
     1021 aaacgaacga ctctgggatt agtaactatt ttattttttt cttcatttct tttttcctct
     1081 ttttctcgat tcagctatct tctttcattt ggatattgat tatttcttct gttgattact
     1141 atccttctgg aattctagac gctgaagtaa ttcacgtttt ctttcttcta gagatcataa
     1201 gtaacgaatg aacgaacttc aaataatttt tttattgtaa ttcatatttg tattgttcga
     1261 tgattcttaa tacgatatga cacaataaat aaaaataggc atggctattt acaacaatag
     1321 tgctatagta gaaaacaaat gaataaggtt acttaaagag gtgaatacga tgaaactgat
     1381 caaactatgg gaagcagatt taaagaaagc ttatcagcta caaactagct ttggaccaaa
     1441 tgagagtgga tttatcaaca atgcttatgg ctatacattt gctgaatttt tagattatgt
     1501 aaaaaaatgc gaaaaaacgt ctttaggaat cgatcttcct gagagttacg ttcccgcaac
     1561 cgtctttatt ttagaaagtg atgaacatga gtatgtagga atttttaatt tgcgccataa
     1621 actgaacgaa acattaagaa acggcgctgg ccatattggt tacggcatct cccctaatta
     1681 tcgtaaaaaa ggatatgcca cacaaggatt agcattagta ctacaagagg caaaaaaaat
     1741 cggcattgat gaggcacttt tgtctgtaaa taaaaaaaat cttggcagtc ttaaagcaca
     1801 acaaaaaaat ggggctgcga tttatcgtga agatgagaaa gaatactata cgaaaatcaa
     1861 actgatgtaa aaaagaagga ttccaaaagg aaagggattt gaatgtcctt tatcaataga
     1921 acacatttat ttaattaact tataatatag cagacaaaat tttataaaca aagaaacctt
     1981 ttatctcttt tcttctagaa aggataaaag gcttttttgt ttttcctatt tgcttctcat
     2041 agctagtcag ccaagatttc tatcgtatcc ccaacgcaat ccgtgcataa cggctcatct
     2101 tatctgtcgt ccatgctgga taccagacta gtttgacttc tgctttcgtt acttctggca
     2161 cttcttttaa tgcgtcatgg atagattctg ttaaaatatc tgctaatgga cagcccatag
     2221 ttgttagagt catcttaatg actgtttctc cattttcagg attgaattct atatcataaa
     2281 tcaagcctaa attgacgatg tcgattccta attcaggatc aatcaccatt tccaaggcag
     2341 caagaatacg ttctttgatt agttctattt cttgtccaga ccgttcttgg tttgcttcgc
     2401 tcatcgtacg acactcctta acacgatttt tatttatcat actctttttt tagctgcttg
     2461 taaatgagaa tttagaagta agattctcgt ttaaatttat aaaaatgttc ttattagatg
     2521 taatagatta aatttattct aaaataaacc tgattcctca ttttttttgt gaacgatttt
     2581 ttatttctgg caatacgcca gttgttcgat acattttttg ttcgttttca tttgcaaacc
     2641 ggccaaatac tgaatgagtt ggtaaaatat tgataaatgc atcgtcgtcg atttcatgaa
     2701 cgatctgatc aagatcatac atttcgtagc gggtgatgac catcatgatc atgttccctt
     2761 gtttttttga atagccaccg acagacggca gtaatgtcat tccacgtacc atccgttttg
     2821 ctagttcttc tgttacctct tctggcttga cggtaacgat cattgctgtt actttttgat
     2881 gactcgtgtg gatggcatca attacttgag tcattgcata aatggaaata atcgtataca
     2941 aggcactttc ccagttaaag aagaagcctg cgatgactac aatgatacca tttagaatca
     3001 gcatgtagtt tccgactgtt tttccagttg tttgtgaaag tacaagagag atgatatcca
     3061 tcccaccagt tgtaaagccc atttttaaag ataatcctac gccaagacct aataaaacac
     3121 cgcctactag ggcattcatc aaaatattat cagtgatctg tccgactgga agcagcatcg
     3181 tcatcaccga tacacataca acattcacaa aactaaggat cgttgctcgt tttcctactt
     3241 tgagaaaccc taatacaaac acagggatat tcaaaagaaa aataaatgtt cctgtgtcta
     3301 tcttgatatg aaaccattca tataaaagcg aggaaatgat ttgagcgatc ccattcatcc
     3361 ctgcagaaaa gacattggct ggaatcaaaa aataatttaa agcgaccgca gcggtcaatc
     3421 cggttatcaa aataaccact attttcttta atctttcatt tttatgatac gcagtcataa
     3481 ttttactcac aagcacctct cccctacttt ggtattccaa atgtaagaat aacagtttat
     3541 ctattaaaat aaaatacttc gaactcaaat gagaagaatt ttagcaaaca ggtaagaatc
     3601 tgccctaatc ctatgataag atagatgtat ggtaaatatt tatatgtata tcaggagggt
     3661 tcctatggca aaagatgcta gcttcgacat tgtatcagaa gtcaatacag aagaagtaaa
     3721 aaatgcgatt cagcaaacaa caaaagaatt gtccaatcgc tttgacttca aggattctac
     3781 agtagaaatc aaattagaga acagcaatct agttgtattg agcgatgatg atttcaaaat
     3841 cgaacagatc aaagatgtat tatttggtaa gctgatcaaa agaaatgtgc caatcaaaaa
     3901 tatccatttt tccgaatcag aacacgctct aggtggcaaa gctcgacaaa ctgccgattt
     3961 ggtcaacgga atcgatcgtg aaaacgcgaa gaaaatcact acagctatca aaaatgaaaa
     4021 actgaaagta aaagcacaaa tccaagaaga tcagattaga gtgacaggta agaaccgtga
     4081 tgatctacaa gctgtgattg ctttattacg taaacttgac ttaccaatcg agttgcaatt
     4141 cactaactat cgttaatcaa aaaacgaggc tgacgtctgt cattcctcgt ttttttgatt
     4201 tactgcttct tttgataacc aaaaacgctg tattccttct tccaattccc caacacttcg
     4261 tacttctctt gctgtttgcc aatgttgtgg gaataaacgt cgataagaaa aactagaaaa
     4321 tcgttgatct gccaatacaa cgattccttg atccgccatg ccacgaatca ctcgaccacc
     4381 agcttgcaag actttgttca ttccaggaag ctgataagcg taagcaaatc caaggtgttc
     4441 cttctcatca taatatgatt tgatcagttc ctgctcatga ttcatctgcg gcaaacctac
     4501 acctacgatc atactgccga tcaatcggtc ccctctcaaa tcgattcctt ctgaaaaaat
     4561 cccgccaagt acgcaaaatc caatcaatgt ttcagcggga tttgtttgaa attttgttaa
     4621 aaatcgttcg cgttcttcct cattcatgtc cgtttcttgg attagcacat tgctgtcggg
     4681 atattgctgt ctgaatgctt ccaccgcaag atctaaatat tgataagaag gaaagaaaac
     4741 gaaataattc ccgacctttc catcagcaaa agcatgaatc gtctctacta attttgccag
     4801 actgtgttcc ctttttcgat aagtcgtctc taggtaattt gcgatgaaaa cttgctggtt
     4861 tccttctggg aaaggactcg gcagtttgta agccagactt ttttgtcccc ccagtgtttc
     4921 ctgataataa gagagtggtg aaaaactagc agaaaaaaga atactgcttt ttcctttatc
     4981 caacgactgc tcaagaaaca agcttggatc aatacaaaac tcttttacta tcagatcgcg
     5041 atacgttttt tcgactgttg tttcgtagtg atcatcataa aattcactga ccttcaaaaa
     5101 atgaagcaaa tcaaaataat aaggaagcaa ttgttcttgt gatgggtgct caggaaattc
     5161 tgccagccat tctttaattt tttcgcttaa ttgataacct gcctttacta aagattcagc
     5221 cggtgctttt tgatgatgat aatcccaatg atcttctttt gctatttctc ggatcgaatc
     5281 aaattctttt aatagtttgt tgaaacggcg atgaagtttt ttgaattcct ttggtatagc
     5341 ttccttactt cgtttcgttt tttcataaga tagttctgcc gaatacatct ctcgggaacg
     5401 attcaccaaa ttatgtgctt cgtcaatcaa aaacagatag tcctcgtttt tttcatcttc
     5461 aaaaaaacgt cttaaataaa cagttggatc aaataaataa ttgtaatccc caatgattac
     5521 gtcacaccag atactgacat ctaatgatag ttcgaaagga caaagtgtat gctttcttgc
     5581 ataagtttcg atgacttcac gagtgatctg attctcatga ttcaaaagat cccataaacc
     5641 ttcattaatc cgattgtaat aaccattggc atatgggcac tgatctggat tacaggtcgt
     5701 ctcatctaaa aagcagatct tgtctttcgc cgtcaaagtg acacttttta cttcagctcc
     5761 tgtatctttt aatgctgtca tagcatcttc agctacttgt ctagttattg ttttagccgt
     5821 tagataaaag atcttttctc cctcttcttc ccctacagct ttcaaagcgg ggaataacgt
     5881 agagattgtt tttcctgttc cggtaggtgc ttctacaaac aatttctgtt tcgtacggat
     5941 cgttttataa gcagctaccg ctagttcacg ctgacctttc ctatactcat catgggggaa
     6001 tttcaaagcc atcaaagagg cattgcgtac agtgcgccag ttttcttgaa aaacaagcca
     6061 ttctttataa tcttcagtca gctttttaaa aaaagtttct aactgctctc ttgtctgatg
     6121 ttcaaccttt ctcgtgatca cttcttctgc cgtctggaag taagtcaatt gcagatttat
     6181 ttcttgtaaa tcatgttctt gactatagat ataggcatac acccttgctt gatgaaagaa
     6241 aagttccact tgttctggtt ctaaatcttc aaatcgtggt tcggaggttt tgatttcatc
     6301 tatataataa ataccgtctc ttttgaaaat accgtctgca cggccttcaa cgatcaggta
     6361 atattcatct aattgaactg ttgtttttaa aaagacttct ttttcatagt tttctcccgc
     6421 atctttttgg agcttgcggt ggattctcgc tccttctaat gcagtatggt tacttttttt
     6481 acgattgtcg atacttccac gtcgcaaaat aaattctact aagtgccgca cagatatttt
     6541 tgactgtttg atcatttcca ccacctcttt ctctctaaga ttacctgaaa aaacctagtt
     6601 tagcaaaaag agctctcttt ccaaacataa aaaaacaccc tgctgattag gcagggatat
     6661 aggatataga atttggattt ttggatgaat ctaatgtaac aaggacatat taaaatgcta
     6721 tgaaaactta ctttccttct ctttagaata tccaaatact tttcatatag tctgataatt
     6781 cagataagta aagagaaata agtacctgtt tttgtttcct agcaatgaat gcgttatact
     6841 aatggatgaa agaagttttg gctatcaagg aggaatgcac atgcatatcg aaagattaaa
     6901 aacaggaatg attgaagaaa actgttattt ggtctacaac gaagaagcac ttttgatcat
     6961 tgaccctgga gaagatgcgg aacgaatcaa acaagaaatc aaaaaaacaa accaacagcc
     7021 ggtagcgatt ttgcttaccc atactcacta cgatcatatc ggtgcagtgg aacctttacg
     7081 cgcgtattat caaatcccag tttatgtcag cccactagag caaaaatggc taggtgatcc
     7141 gattttgaat ctttctggac tcggtagaca tgatgatatt gctgatgtta ttgtctcgcc
     7201 tgcagaatat gaatttgaaa tgaaaactta tactttagga aacatgtctt ttcgcgtcgt
     7261 ccctacccca gggcattcta taggaagcgt cagctttatt tttgatgatt ttgtcgtttc
     7321 tggcgacgcc ctgtttagag gaagtatcgg tagaaccgat ctgtatacag ggaatttaga
     7381 acaattactt catagtattc ggacccagct gtttgtatta cctgatgaat ttgcagtcta
     7441 tccaggacat ggcgatgcaa cgacgattga acaggaaaaa aggacaaatc cttttttcaa
     7501 tcgttaaaac gacttaggag gagaataaaa tgacagaaac tacactttat atcgtacgac
     7561 acggaaaaac gatgttcaat acaattgaac gagtacaagg ctggtgtgat acacccctca
     7621 caaagcaagg acaagaaggc attcattatt taggtcgcgg gttaaaaaat gtcgactttt
     7681 catttgctta ttctagtgac agtggtcgtg caatcgaaac tgcccgtatt attctaggag
     7741 aacattctaa gggaaaagaa attccttatt ttattgaaaa aaggattaga gaatggtgtt
     7801 tcgggtctct tgaaggtgga tatgacatgg aaatgtgggg tgtgatccct cgtgtattga
     7861 attttaaaac atatgatgaa atgtttacca cagaagtttc ttttgaacaa attgctaatg
     7921 ctgtatacaa cttagatacc gctgggtggg cggaaactta tgaaacattg cgagaccgtg
     7981 tatggacagg atttgaagat atcgcccatc actgcgaaaa aaatggtggt ggaaatgttt
     8041 tagtcgtatc tcatggtttg acgatcgcct ttttgcttaa tctgatcgat cctaaacagc
     8101 ctgttcgtgc tgggctttta aatggcagcg taacaaaggt cacttatgca aacggtcgat
     8161 tttctatcca cggcatcaat gatacgtctt atatcgataa cgggaaaaca catagtcgcg
     8221 actcgtttct ataatgggga tattttaatt tttaccagag aatcataaaa ggcaaagtct
     8281 gaacgagggc tttgtcttct tttttctctt cgtgtactta tctgctttat ttctcaagtt
     8341 tttcttaata ttaaaaaaca gattgggttt ctcaaaaaat aacggtatac tataagatgt
     8401 atcttaattg ggaaggaagg aatttatgtt ttttgcaaat attatcaaag cagtgattct
     8461 cggtattatt gaaggaataa cggaatggct gcccatcagt agtactggtc atttgatcct
     8521 agcagatgaa ttcatcaaac tggatgccag ttcacaattt atgtcgatgt tcaatgtcgt
     8581 tatccaatta ggggcgatct tggctgtcgt cgttctttat tttcacaagt taaacccatt
     8641 ttctccaagt aaatcaacgg tagaaaaaaa agatacgtgg acactttggt ttaaagtgat
     8701 cgttgcttgc ataccagcca tcatcattgg tttgcctttg gatgattggt tagaagcaaa
     8761 tttccacaag ttcttaccag ttgccattgt tttgatcgtt tacggtattg cctttattat
     8821 tgtcgaaaaa cgcaacaaaa acaaaacacc tcgttggact tctctgaatg actttacgta
     8881 ccaagcagct cttcttgttg ggttattcca agtcttggca cttgttcccg gaacatcccg
     8941 ttctggcgca acgatcctag gatctatctt gattggtgct tcgcgttttg tcgctacaga
     9001 gttttcattt ttcttaggga ttcctgtcat gtttggcgca agcggcttga agatcgtcaa
     9061 atatttagct gaagggaatt cattcaaaat ggaagaaact gttgttcttc ttgtaggaac
     9121 attcgtatct tttgtggttt ccgtcttagc tatcaaattc ttactaagct atttgaagaa
     9181 aaacgacttt accgcttttg gctggtatcg tattatcctt ggtatcattt tgattggtta
     9241 ttggtttatt gcaatgtaga aaaaaccaga acacttctct t
//
LOCUS       DARBEC010000102         8888 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.106, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000102 DARBEC010000000
VERSION     DARBEC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.106"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            857..1768
                     /locus_tag="SCL80_002426"
     CDS             857..1768
                     /locus_tag="SCL80_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-relaxation family protein"
                     /protein_id="HEG1108266.1"
                     /translation="MKYLLHGYFIDYSTRNSSTLAILCLLHELRVVTRKQLYKLLNFD
                     RSISKQGVTNALVRLYEKQLIDRKKYGTEMYYYLTKEGHQSIGGYYTLPKVPEYNLNH
                     HLQINNYLIKGMALAKDHPHLKRIISERRTVYETKDLNPKTKGRKFFVPDFIFRFRDK
                     ENHEINWSFEIELTLKTRRRYREAIFPKYIRELRTRPESHLIYVTPSGAIQEELHAFK
                     RIFFKRETEKDASQQELFERLHIFSALEFEEGLKALLENDPAVNWRTVEREKIYPNGA
                     VQSRLNLFEESTENEEKPKVKFTPEVL"
     gene            2255..2521
                     /locus_tag="SCL80_002427"
     CDS             2255..2521
                     /locus_tag="SCL80_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001245899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108267.1"
                     /translation="MTTKNEQLKEQARKELQERGLIIEGSFEGDFETYIGCYARPINK
                     PTALDPMNEQEVKEQEKHAVNGFPQDFTEWYEWEINNGKLENFL"
     gene            2593..2934
                     /locus_tag="SCL80_002428"
     CDS             2593..2934
                     /locus_tag="SCL80_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108268.1"
                     /translation="MAKNTPYQVGDIFYSSWGYEQTNVTFWQIVKLTEKTAWFQPIQK
                     KTVEHYSSMSGKVVPMKDKFIEYPLAKTKDSIVKKRIDPETYPDMLCGYRSWDTFHRY
                     KNQAVYESSYY"
     gene            2989..3408
                     /gene="ssb"
                     /locus_tag="SCL80_002429"
     CDS             2989..3408
                     /gene="ssb"
                     /locus_tag="SCL80_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEG1108269.1"
                     /translation="MMNSVNLVGRLTKEVDLKYTPKGNATGTFILAVNRNYTNANGER
                     EADYIRCVIWRKAAETLAKYTGKGSLIGINGRLQTRSYQNQQGQTMYVTEVLVTDFYL
                     LESKEINEQRSKSMANNQNNSSIPGNEIQISDSYLPF"
     gene            3485..3781
                     /locus_tag="SCL80_002430"
     CDS             3485..3781
                     /locus_tag="SCL80_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108270.1"
                     /translation="MKIIDSYDQRTMQYLGSFDYTDENIINYVASLPPYQSVRLVDFA
                     SDELVLTTMGNFLDYVGDKKWLEKILSKLIAKQTGEEPINAIQLFNRYEQENND"
     gene            3796..4227
                     /locus_tag="SCL80_002431"
     CDS             3796..4227
                     /locus_tag="SCL80_002431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEG1108271.1"
                     /translation="MKRLKRSIFIVLALIDFVGTGCEAIDVQKAPLSEIKQAITKQDE
                     EQPLNTQVEIVNQNKPSFSEEDFSLENGTRQFFSDLDRLNRVGVASALLHKSRMPTEE
                     RDRLYIKPTGWKQKKMKNGDYLYNRCHLIGYQRATSFPISM"
     gene            4409..4714
                     /locus_tag="SCL80_002432"
     CDS             4409..4714
                     /locus_tag="SCL80_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108272.1"
                     /translation="MKLDMPGRYKLLRVRLFLIKLTNLRMYGSITDLYSKDIRLRMQR
                     TLSKSKNVGHEIRDGRLAVYLSRVGKTCLIDSDESGNATKRFTEEIEQLAELNIRYF"
     gene            5118..5606
                     /locus_tag="SCL80_002433"
     CDS             5118..5606
                     /locus_tag="SCL80_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEG1108273.1"
                     /translation="MKKTIFVLAIFICFGLLLVGYLLFNSNEMRTISTNDLLKKSELK
                     DKKKESLAFIMFTQEECEYCIAINPIIDSVNKETLNKIKKYDISVDFQSKQKCKIKFV
                     PTIVAYSKNKEVGRIDNELDNLYRKKQDGSIVIDKKKTKTLLIDWMKKMDKQGEYLYG
                     ED"
     gene            6940..7242
                     /locus_tag="SCL80_002434"
     CDS             6940..7242
                     /locus_tag="SCL80_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108274.1"
                     /translation="MTKLGITLDDNIYDEKILLSGGEKQKISLARALIKSASVIILDE
                     PFRNLDNETRDNLRDYILNTKKEVCYIIITHDHILDNFLDSNDEILYLNKEYDNNE"
     gene            7235..8263
                     /locus_tag="SCL80_002435"
     CDS             7235..8263
                     /locus_tag="SCL80_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEG1108275.1"
                     /translation="MNSKKLQMGEIVRGPSQISIDVTYKCNFRCKHCYNASGGDNCFM
                     DNELTDDELLELTKDICNLKPYNICFCGGETLLRKKIVLSCATLLKEAGIPNIAIVTN
                     GSLVTQDVAKELFKAGINRFQVSLDGKNASSHEALRLYPNSFEKAINAIKLFRELSEE
                     VDVAFCPTSFNYNQLREVYQLVNTLKVKSLRIQPFMLSGRGMQNREIVPTVSQYRKLV
                     SDINYIKRNEDYTKIDWGDPIDHIIRHRTLLKDFSNFVSIRSDGGLMASPYLPIQVGN
                     IKCHSLSKYWRAGLYKVFSLDLVQDIATQVSSIQDMSKELKKGHIKWLNEDIEIDILD
                     EYKHDILI"
     gene            8319..8537
                     /locus_tag="SCL80_002436"
     CDS             8319..8537
                     /locus_tag="SCL80_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108276.1"
                     /translation="MISEPQMNNMYESPTIMGAGEAAPRACSAIMVCVLLASAGVGIQ
                     AGAVYTYAVVATVAVAAAAVWKTVGLVG"
     gene            8608..>8888
                     /locus_tag="SCL80_002437"
     CDS             8608..>8888
                     /locus_tag="SCL80_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108277.1"
                     /translation="MKYNNLKPKLLNENIEYIREENYGYLTIVPKNGTELKDNVFNPT
                     VRYILANCTGKNSVKKIANGLKKKFPSLEYEKFYQDVVGTIKFFKHYNI"
BASE COUNT         3340 a         1174 c         1706 g         2668 t
ORIGIN      
        1 acaaattcac tcattttttg agcaaaaatg tttcaaaatt tgaacaaaaa ccaattgaaa
       61 attgaagcaa atttcactcg aattgaatcg attttgagct aaaatcaata gaaaaaataa
      121 acgtattttg tttgaaatcc gtttattttt tttacgtttc agtattagta aggattattt
      181 gagggttatt tagagattat ttaaaggtta tatgtaataa gtggggggag ggctgtcggc
      241 gcttggtatg aaaggcattc tccgacttcc aggtttttga ccattttctc tcaacgtgga
      301 aaaaccgcca cttatgagag aaaatggtca aaaacctaag cgggaagtct ccgaataaca
      361 taccaagcgc cgacagccaa cagataaaga atacactact aaaaatagta gtagcataaa
      421 atttaaaagg gaaaaaatca acccctaccc cagtcttggg gtctcaaaac taactccccc
      481 ctaaccccct ctttcagcct cttttgaaag agggggagaa tcaaccccac ggccaacgcc
      541 ccagtgttct tggggctgtc cagaacagaa acttgcgaga gagtgggggt aaaacaagtg
      601 tagaggaaac ttgaagactt gttcgaacag aaacttgcga gagagccgga gtaaaacaag
      661 tgtagaggaa acttgaagac ttgttcgaac agaaacttgc gagagagttg gagtaaaaca
      721 agtgtagagg aaacttgaag acttgttcga acacaaactt acaagagagg ggagtaaagc
      781 agttgcagag gaatcaggaa gatttgttcg aacagaaact tgcaagaaag cattattaaa
      841 ttgaaggtga tgaaatatga agtatttatt gcatggctat tttattgatt attctactcg
      901 caatagtagt actttagcga ttctatgttt gcttcatgaa ttgagagtgg ttactagaaa
      961 acagctatac aaactattga attttgacag atcaattagt aaacaaggag taacgaatgc
     1021 tttagtgaga ttatatgaaa aacagttaat cgatcgaaaa aaatatggaa cagaaatgta
     1081 ttattatttg acgaaagagg gacatcaaag cataggtggg tattatacgt tgccgaaagt
     1141 tcctgaatat aatttgaatc accatttaca aattaacaat tatttgatta aaggaatggc
     1201 attggcaaaa gaccacccac atttaaaacg gattatttca gaaagacgta ccgtctatga
     1261 aacgaaggat ttaaatccta agacaaaagg aagaaaattt tttgtgccag attttatttt
     1321 tcgttttcga gacaaagaaa atcatgagat caattggtcc tttgaaattg agctaacgtt
     1381 gaaaacacgt cgtcgctacc gtgaagcgat ttttccgaaa tatattcgtg agttgcgaac
     1441 acgtcctgaa tcacatttaa tttatgtgac acctagtggc gcaattcaag aagaacttca
     1501 tgcgtttaag cgtatttttt tcaagcgcga aactgaaaaa gatgcgagtc aacaagaatt
     1561 gtttgagcgg ttgcatattt tttcagcttt agagtttgaa gagggattaa aagcattatt
     1621 agagaatgat ccagcagtca attggcggac agtagaacga gaaaaaattt atccgaatgg
     1681 cgctgttcaa agtcgtttga atttatttga agaatcaact gaaaatgagg aaaaaccaaa
     1741 agtgaagttt acgcctgaag tattgtaaaa gagggtgttc gcttcgctct ttttttgtgc
     1801 cagtttgctg gcaccgttca gacgcaagcg tctgaaccgg ctcgttttta ggaaggacta
     1861 acttgtttga tttatccgtt gtcaaagaag tggacgttcg agagtcccta atcgtatagc
     1921 agtcattcgg cgagtataac ctaaaacata acctgccaat tgccaagggt aaaatgcacg
     1981 ctgcgcgccc ttggcaatcg tcagaacatg ttttcgagcc ggttatgccg aacgaccgct
     2041 atacgaaaag ggcctctaaa cagaagtcca ctttgatcct tgacaactga aacccaaaat
     2101 caaacaagca ggccttcgtc taaaaacgag ccgaatgaag accttggggg ttgcaccccc
     2161 aaacccccgt gcccccgaca cactaccgaa aaggtggggg tgggtggcga aaaaatcaaa
     2221 taaaacattc tattcaagaa aggaagaaac aaccatgaca actaaaaacg agcaactgaa
     2281 agaacaggca agaaaagagt tgcaagaaag aggtttgatt attgagggga gctttgaagg
     2341 agattttgaa acctatattg gttgttatgc aagaccgata aacaaaccta cagccttaga
     2401 cccaatgaac gaacaagaag taaaagaaca agaaaaacat gcagtgaatg gttttccgca
     2461 agatttcact gaatggtatg agtgggaaat taacaacggg aagcttgaaa acttcttata
     2521 aaagtcagat gacgtgcaaa gagaatgatt ctctttgtac ggtaaaaata taaagaaaga
     2581 aggaaaaaga aaatggcaaa aaacacacct tatcaagtag gagacatttt ttatagtagt
     2641 tggggctacg aacaaacgaa cgtgactttt tggcaaattg tgaagttaac agaaaaaaca
     2701 gcatggtttc agcctattca aaagaaaacg gtcgagcatt attcgtccat gagtggaaaa
     2761 gttgttccaa tgaaagacaa gtttatagag tatcctttag caaaaactaa agacagtatt
     2821 gtaaaaaaac gaattgatcc agaaacctat cctgatatgt tatgtggtta tcgtagttgg
     2881 gacacgtttc atagatataa aaatcaagct gtttatgaaa gtagttatta ttaaggggat
     2941 agtttcccct ttcaaaaata ataaatagaa gaatggagag aaaaaatcat gatgaattca
     3001 gtgaatttag taggacggtt aacaaaagaa gtggatttaa aatacacacc aaaaggcaat
     3061 gcgacaggca catttatttt agctgttaat cgaaattata cgaatgcaaa cggcgagcgt
     3121 gaagcagatt atatccgctg tgtcatttgg cggaaagctg cggaaacttt agcaaaatat
     3181 actggaaaag gaagtttgat tggcattaat ggacgtcttc aaacgaggag ctatcagaat
     3241 cagcaaggac agaccatgta tgttacggaa gttctagtga cagatttcta tttgttagag
     3301 agtaaagaaa tcaatgagca acggtcaaaa tcaatggcga ataatcaaaa taattcttca
     3361 attccaggaa atgagattca aatttcagat agttatttgc ccttttaagt gaaaaataat
     3421 tttgttctat acctgatttc ttcaggtata gaacaaaaaa taaatagaga tgaggcgatt
     3481 ttttatgaaa atcattgaca gttacgatca acgaaccatg caatatttgg gctcgtttga
     3541 ttatacagat gaaaatatta ttaattatgt tgcaagttta cctccttatc aaagcgttcg
     3601 cttggtagat tttgcaagtg atgaattggt attaacaacg atgggcaact ttttagatta
     3661 tgtcggagac aaaaaatggt tggaaaaaat tttgtctaaa ttgattgcga aacaaacagg
     3721 agaagaaccc ataaatgcga ttcaattgtt caatcgatat gaacaagaaa acaatgatta
     3781 aagaggagag aaaaaatgaa aagattaaaa cgaagtattt ttattgtgct agcattgatt
     3841 gattttgttg ggacaggttg tgaagcaatt gacgttcaaa aagctccctt atcggaaata
     3901 aaacaagcaa taacaaaaca agatgaagaa caaccgctaa atacacaagt agaaatagtg
     3961 aatcaaaata aaccgagttt ttctgaagaa gatttttcgt tagaaaatgg aaccaggcaa
     4021 tttttttcag atttggatcg tttaaatcgg gtaggtgtag caagtgcact tttgcataaa
     4081 agcaggatgc caacagaaga acgagaccga ttatacatta agccaactgg ctggaaacaa
     4141 aagaaaatga agaatggcga ttatctttat aatcgttgtc atttgattgg ctatcaacgt
     4201 gcgacaagtt tccccataag tatgtaatac gaaacgggag gattaatctt aagattaatc
     4261 acaactaacc tatttgacag ttggaatgaa agctatcatg tctgctgaat caactggtct
     4321 gatactccta catgcaataa agtctactag gaagttattg tatgaagcta ggtaaaggcg
     4381 gcagaacaat ttctaagtgt ggaatcatat gaagttggat atgccgggac gctataaatt
     4441 actcagggtg agactgtttt taattaaact gacgaacttg cgaatgtacg ggtctataac
     4501 cgacttatat tcgaaagata taagattgcg aatgcaaaga acattatcga aaagtaagaa
     4561 tgttggtcat gaaattcgag atggaagact agcggtgtat ctgtcaagag ttggtaaaac
     4621 atgtttgatt gattcagatg agagtgggaa tgcgacaaaa cgttttacag aagaaataga
     4681 gcaactagca gaactgaata tacgatattt ttaaatactt tagcggagaa taaaaaaatc
     4741 ctctacagaa aactacggag ggcaacatgt gctattgttt caacaataca tttttaattt
     4801 ttaccataac aaaaaagtac ttgaaaaaaa acagaaatat gttaaattaa aaatgaaaaa
     4861 atggaagcgg ttacttattc ttttgaatct aagataaaaa ttcaaaagaa taacataacc
     4921 caaatatgat tatacttgag attctaaata tttatcgaaa aggaatttgt tttaaggaaa
     4981 aatgattcta tggacggtta ttgatttata tttaccaaat ggctaataaa atagtatttc
     5041 ttatattgta tagtttttgt ttttatgtga aaacatgagc aatagaattg tactagtagg
     5101 ataaggatgg atggaaaatg aaaaaaacta tttttgtttt agcgattttt atatgttttg
     5161 ggctactttt ggtaggctac ttgttgttta attctaatga aatgagaact atttctacga
     5221 atgacttgtt aaaaaaaagt gaattaaagg ataaaaaaaa agaatctctt gcctttataa
     5281 tgtttactca agaagagtgt gagtattgta tagcaataaa tccaatcata gatagtgtaa
     5341 ataaggaaac tttgaataag ataaagaaat atgatatatc tgtagatttt caatctaaac
     5401 aaaagtgtaa aattaagttt gttccgacta ttgttgcata tagtaagaat aaagaagtgg
     5461 ggagaattga caatgaatta gataatttgt atcgaaaaaa gcaagatggt tcaattgtaa
     5521 tagacaagaa aaaaacaaag acattgctta tagattggat gaaaaaaatg gataaacaag
     5581 gagaatattt gtatggagaa gattaaacag acatttccgt taattaataa atattggaat
     5641 aagaaaaaaa tttttatatt ttttattacg tttctgctgt catctttatt ttctctaatt
     5701 ttcccgtatc tatttagtaa tgttattgat ttatatttag agaatgagtt atcacagaat
     5761 attgtgcgaa tagtaacgat tcttatattt cagttatttt tagtcttaat taaagaagtg
     5821 tatggatata taaaaactat ttatgcattt gaagttgatt tatcaatgaa aattgatttt
     5881 cttaaggttt tacaaaaaaa aaatcttttt ttttcgcaaa cgaagcattc aggagaaatt
     5941 caatttagga tgttttcgga tatttctact atcgtaaata ctatctttat tttttctgtt
     6001 gaattacctg tgataggcgt tttagttctt gtagcggtat ttattataag taaagtgaat
     6061 cttatttttc tgattttttc actaataatg atgggagcta tggtgttaca gtataattta
     6121 tttcaattac cgcttaagaa aacaatcgag aatacaaaaa gtgttaatca aagtattaca
     6181 ggagaattga atgcacattt tgatcaatta gaaattataa gcgcatataa ttttgagaat
     6241 atctctattt taaaattcga ttctagattt aaagaattat ttaatgtttt caaaaaacaa
     6301 aataaattac taacactggg aagctgtttg gcacaggtta ttttccaatt atggattgga
     6361 ggaatatttc ttattgatgt tattttatta gctaaaggaa caataagtat tactaatttt
     6421 gtaatagtat ttatgtacgc taacatggtc cctactttat ggactacttt aattagtatt
     6481 atttggaaat ggcaagacta ttcagtgtca ttaaatagat attacgaata ttattcaaat
     6541 aacaacaaag tggctggcac aaaaaaaata aataaaataa attctattac tggtcaatca
     6601 ttaaattttt catataacaa aagaaaggta ttaagcaata taagtttttc aactgaaatg
     6661 aataaactat tcatgatatc aggagaaaat ggaacaggga aaacaacact gatgaaatta
     6721 tttactaaac agttgccggt tagtaataat caattattcg tcaacgggat agatattaat
     6781 atgattgata acgaatcatt aagaaggtta atttcaatcg caacacaaat accatatata
     6841 attaatagct ccgtgaagga gaatatttta atggggacta aaaactatga taaaaaaaaa
     6901 ttcagtaaat taatagaact attgggaata agtaaattta tgacgaaatt aggtattact
     6961 ttggacgaca atatttatga tgaaaaaata ttattatctg gaggagaaaa acaaaaaata
     7021 agtttagcta gggcgcttat taaatcagct tcagttatta tattagatga accatttaga
     7081 aatttagata atgaaactcg cgataatttg agggattata tactaaatac aaaaaaagaa
     7141 gtttgttata taattataac tcatgatcac attctagata attttttgga ttcaaatgat
     7201 gaaatattat atttaaataa ggagtatgat aataatgaat agcaaaaaac ttcagatggg
     7261 tgaaattgtt agaggtccta gtcaaatatc tattgatgtt acatataaat gtaattttag
     7321 atgtaaacat tgttacaatg cgagtggtgg agacaattgt tttatggata atgagttgac
     7381 tgatgatgaa ctgttagaac ttacaaaaga tatatgtaat ttaaagccat ataatatttg
     7441 tttctgtgga ggagaaactt tactgagaaa aaaaatagtt ttatcttgtg ccactttatt
     7501 aaaagaggca ggtattccta atattgctat tgttactaat ggatcattag taactcagga
     7561 tgttgcaaaa gaattattca aagcaggtat taatcgattt caagtaagtt tagatggaaa
     7621 aaatgctagt agccatgaag cacttagatt atatccaaac agttttgaaa aggcaattaa
     7681 tgctatcaaa ttatttagag aattgtccga agaagtggat gttgcttttt gtcctactag
     7741 ttttaattac aatcaattac gagaagtata tcaactagta aatactctta aagtgaagtc
     7801 tttaagaatt caaccattta tgcttagtgg taggggaatg caaaataggg aaatagtgcc
     7861 aactgtttct caatatagaa aactagtttc tgatattaac tatattaaaa gaaatgaaga
     7921 ttataccaag atagattggg gagatccaat tgatcatatc atccgacaca gaactttatt
     7981 gaaagatttt tcgaattttg tttctattag gtcagacgga ggattaatgg catcacctta
     8041 cttgccaatt caagttggaa atattaaatg tcacagtctt tctaaatatt ggcgagcagg
     8101 actttataaa gttttttctt tagatttagt tcaagatata gctactcaag tttcttcaat
     8161 acaagatatg agtaaagaat taaaaaaagg acatattaaa tggcttaatg aagatataga
     8221 aattgatatt ctcgatgaat acaaacatga tattttaatt taggtttatc attggaaagt
     8281 ttagataaac aaagtacttt tagaaaggag ggaaaaaaat gatttctgaa cctcaaatga
     8341 ataatatgta tgaaagccca actataatgg gagctggtga agctgcgcca agagcttgtt
     8401 cagcaattat ggtgtgtgtg ttattagcat ctgcaggagt aggtatccaa gcaggagcgg
     8461 tgtatactta tgctgtagtt gctacagtag ctgtagctgc agccgcagtt tggaaaacag
     8521 taggattagt tggttaggaa ttaataggag actaactgtc tcctattgat tccaatgatt
     8581 aagctaagga ggaattttaa gaaaataatg aaatacaata atttaaaacc gaaattactt
     8641 aatgagaaca tagaatatat aagagaagaa aattatggat atttaaccat agttcccaaa
     8701 aatggaactg aattgaaaga taacgttttc aatccaacag taagatatat attggctaac
     8761 tgtactggaa aaaacagtgt aaaaaaaata gcgaatggtt taaaaaagaa gtttcctagt
     8821 ttagaatatg aaaaattcta tcaagatgtt gtaggaacta ttaaattttt caaacactat
     8881 aatattat
//
LOCUS       DARBEC010000103         8818 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.107, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000103 DARBEC010000000
VERSION     DARBEC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8818)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8818)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8818
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.107"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(943..1356)
                     /locus_tag="SCL80_002438"
     CDS             complement(943..1356)
                     /locus_tag="SCL80_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEG1108278.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1812..2297)
                     /locus_tag="SCL80_002439"
     CDS             complement(1812..2297)
                     /locus_tag="SCL80_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108279.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2436..2651)
                     /locus_tag="SCL80_002440"
     CDS             complement(2436..2651)
                     /locus_tag="SCL80_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEG1108280.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2875..3675
                     /locus_tag="SCL80_002441"
     CDS             2875..3675
                     /locus_tag="SCL80_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1108281.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIKEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3694..5562
                     /locus_tag="SCL80_002442"
     CDS             3694..5562
                     /locus_tag="SCL80_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEG1108282.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELYDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5555..6046
                     /locus_tag="SCL80_002443"
     CDS             5555..6046
                     /locus_tag="SCL80_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEG1108283.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6034..6588
                     /gene="srlA"
                     /locus_tag="SCL80_002444"
     CDS             6034..6588
                     /gene="srlA"
                     /locus_tag="SCL80_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEG1108284.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6606..7601
                     /locus_tag="SCL80_002445"
     CDS             6606..7601
                     /locus_tag="SCL80_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEG1108285.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVPTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
     gene            complement(7743..>8818)
                     /locus_tag="SCL80_002446"
     CDS             complement(7743..>8818)
                     /locus_tag="SCL80_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEG1108286.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT         3047 a         1425 c         1682 g         2664 t
ORIGIN      
        1 tttgttacta tgaataacat aataagaaag taaagtggat tgatttgaga tctttttata
       61 cttcaaaaaa gataacaccc aactttcaaa aatatgtttt ggaagttggg acaaacgagt
      121 taatacattt taatacatta ttttttattc aaaaaaaata gcacaatggc aaagatcgta
      181 agagagatca taagccccaa tgaatgacta aaaagaaaag cagaatcaca tgccatatca
      241 tcccatattt cttcaagaaa ataaataagc gggaaaaagg gaaggaatag tagaacatcg
      301 atccagcacc atttttttct tcttttgaaa taacataaaa caataaacat gaacgtgcag
      361 ggaagtaaaa acaaagcttt actcataact aacaaaatac cagctccttt tatttttttc
      421 tcccccacat tcaaagagta acaatggtat gtacttaata atagaaaaat ggatcaactt
      481 gtatgaataa cagctgaaaa gatctgcacc aaataaaaaa cttccagcca aaactgactg
      541 gaagtttatt tttttctgaa atattctaac acaaatatga aaaagatggc cgaagctaat
      601 aagccgattt cgtagcaaaa caatgagata ccagagtcgt aataataaaa tcctctaatc
      661 ttatggataa aatagatcat cggacaagca gataagagta agtatttgtt aggccttttg
      721 aataagtaaa agaaagcact ggtcattatg ataaatggta tccaaaacaa aagtttactt
      781 aagacaatca aatgatcaac tcccatattt tttaccaaga gagatcataa catctttatc
      841 gtaaaaacaa aatatttttt tgctcatttt gaaaatttaa acatactcgt tcttatccaa
      901 atatataaaa atcgcccctc attcatcgag aggcgataag ttctaattta actcaatttt
      961 ttttgtttct tttggctgat aagctgtttt aggtaatacc aatttcagca caccgttttt
     1021 aaaggttcca gtgatttttt cttcatcaac attaggaaga tagaatgaac gtttgtaaga
     1081 gaccgaatgt ctttctttcc gtacatagtt gccatcttct ttatcttctg tatgggactc
     1141 atgattggcg gagatcgtca aagtattatc tgaatattca actgtcgtgt cttctttgtc
     1201 acagccaggc atatcagcag ttagtttgta ttctttttca ttttcgacta aatctacttt
     1261 tggataactt gaaacaccta aaaaatcatt gaatgctgga ctaaacacgt cattgaaatc
     1321 agggaacatg tcgcgaatgc taggtacatt tgccattttt gatcatcctt tcaataagta
     1381 ttttttattt atccctattt acgtaataac tctattttga aaaaaatgca agtataatgg
     1441 ttagctttgt gaatatttca tctaatacga tacaaaggct atctttctaa tttgaaaaag
     1501 ggatcattaa aataataaga agaaaatatg tcaaaaggta aaaaagtaaa ttcttctatc
     1561 caaatataga aaaattgaat ctagaaaacc gcccgacaat aaatttcatt aactcagttg
     1621 accgaaaatg aagtagatac caaaaattta tcaagagagc aagtagagaa gtgagtatat
     1681 tcataagaga aatagctgta acgaattatt aggttaatgc aaatgcttat ttatactccg
     1741 tataactttc tcagaaaaaa tgataccaat aatgacttgc tagtactgta aatccgtata
     1801 atatcatcta atcaaaatgg tgttccattt gcatctgtca tcgtaatcga tccttcgggt
     1861 gacactcgat aaaaaccaac gcttccagat cctccttggg aacggatttg tttgctggaa
     1921 gctgtgaaca aataatctcg atcaattttt tgataaaagc taataacaat atcgtcactt
     1981 tgtaattcgg aacggtgggc aataagaaat agaagtgctt tgtcagagtt ttctaatgga
     2041 agagaaggta cagtgctttc ttgcatttca gatgttgaag aaatgctaaa cgactgaata
     2101 cttgattggc tatgttgcga atctacatta ccagtagaag tattagtaat ggaagtagta
     2161 gaagtacttt tagttgaacc aacctcagtt gttgaaatgg ctgctgaaga ttctttaaca
     2221 gaattctttg ttttatatcc acaggcagaa aaaagtatta cagttcccaa caagcctgcg
     2281 taaatccctt tatgcatgat agctcctcct aaaatagatt atctttattc agtatatcac
     2341 ttaaacaaag aaaagcagat ggtttaaaat gaaaaaacct tcccaataaa gggaaggttc
     2401 atgaagataa taactttttt aataattata gctttctaat atctttcatc aacttttcta
     2461 gtaatcattg ggaaaaattt caagcagtag atcgcacaaa tacctacgac gatataaatg
     2521 atcttcgcaa aaatagttgt tgaaccacca gcaatcatag ctactaaatc aagttcgaac
     2581 aagcctacca acagccagtt caaccctccc acaatcaata aagttaatgc aatagcatct
     2641 aaagttttca ttagaattct cctttcttaa atgataaaac aattatatat caaaaagaga
     2701 aacattgctt atataacgct caggaatttt agcaatctat gcaaaaagat gaaaattttt
     2761 tttgctcttc cattagtaca cattttttag ctgaagctgt aaatttcatg tcttaatttt
     2821 ttttagtgag cttttatact ggatttgtaa gaaaaaagaa ggagaggatc acatatgtcg
     2881 gattggttgg atattgcagg aaaagtagta attgtaactg gaggttcctc aggaattgga
     2941 agaagtatcg tcgaaaattt gttgaagcag aatgcacaag tagcgaattt tgatgtaact
     3001 gaatgccgta tacagcatga aaacctacta tctttaaaag tcgatgtgtc ttctaaaacg
     3061 gacataaaag aagggatata taaagtaatg aaacatttta agacgatcga tggtctagtc
     3121 aataatgcag gaataaatat tccttctctt ctaatagata gaaatcatcc aaaaagcaag
     3181 tatgaattat cagaacaagt ttttgataag atgatcgctg tcaatcaaaa aagtgtatat
     3241 ttgatgagcc aagcagttgg aagaatcttg gtgcaaaagg gatcaggagt gatcgtgaat
     3301 ctttcttctg aatcaggatt ggaaggatca gaaggacaga gctgttatgc tgctacaaaa
     3361 gcagcgatga acagttttac tcgttcttgg gcaaaagagt taggaaagag gaatgttcgt
     3421 gtggtaggtg ttgcaccagg gatactggaa gaaaccggtt tgagaacaca agaatatgaa
     3481 gaagctcttt cttatacgag aggtataagc gtcgaacagc tgagaaacgg ttacagtcga
     3541 acaagtacga tccctctagg aaggagtggt aagctgcaag aagtggcgga tctagtctgc
     3601 tattatcttt cagaaagatc aagttatatc actggagtca cgactaatat ttcaggaggt
     3661 aaaacaagag gataaaggat aggggggacg cacgtgacgc tagtaaacag atggtaccaa
     3721 ttattgaaat tactagtcac tcacaagaaa gtttcaatcc aggaagtgca aaaagaaatc
     3781 aaaaccagta atcaaaccat aaaaaaagat attggggagt tgaatgaaga aattgccggt
     3841 attgcaaaaa tcatccaaaa aaacaaacat tttgaacttg aaatttatga ttttgatttt
     3901 tttgatgaga tcatgcaagg aaaattgaaa acagccagtg atttcaattc ggcatccaaa
     3961 agaatcgctt atttactaaa taggctgatc gaaacagagg atttcttacg tatggatgat
     4021 ttgtctgaag aagtcggtgt cagcagagga acagtggtca aggatctaaa gacattaaaa
     4081 gagatgatca atgattttga tgtaaagatt acaggcacac caaataaagg actaagaata
     4141 ataggagatg agctagaatt acgactactt ttattttatt ttgtttctcc ctacttttca
     4201 gagacgaaaa cagaacagtt tctagaagaa aaacaagcaa tcaaacattt tcagaagaag
     4261 acgaatgcta gtaaacaaga aatcaccctt ttaacgaaag ttatcggtat ttctttgaat
     4321 agaatctcta gtaactatct gttgaagaag cccatcaatc gttacagtgg gtgttttgaa
     4381 tatatccaag aattcaatga actgatcaat cagttggaag aaatctatga actaactttg
     4441 agccaatatg aaaaagattt tatcagtttt cctttgaaca tcatcaacac aagtataacg
     4501 atgataagga aagctaactc tgatttgcgc ttatatttcg accccatgat gatgcgtatt
     4561 cgacgattag atctcattga tctggatgag tccttcttat atcaagagat gaaacagcac
     4621 ctctctttat tgataaatcg ggtaattttt cgttatcgat tgactgattt attttatggt
     4681 gagatagaaa aaaagtatcc tttttcttac caaatcgcta gcgaatgcat acaagcatta
     4741 gaagctatct tgcaacataa gatctctaaa gtagagatca gctacctaac gttgtatttg
     4801 gagttagcat ttcgatatca aaaagagcat ttttcctcta aaaaagtagc cattgttcat
     4861 acgacaggaa aaggaacagc attaatgatc caaagacaaa tcaaaaggat attggggagg
     4921 gatatacaaa taacacatgt ttcagaagaa caatatgaaa aagtagatct aaacaaatat
     4981 ttcgctgttt ttacaacgat tccgttaaaa aatgtaaatg aagagacacc ggtgatccat
     5041 ttgactaatc tttttgatga ctcttggtta agggcacaat ggcaaaaagc aaaaaaaaag
     5101 cgattagccg ggtttcattc tactgttttt ttctatcaaa tatggaaaga aaaaaagacg
     5161 tatcaagaat atttaaacaa attagtcgct cctttgatca gaggctatta tgtggatgaa
     5221 gaatttgaaa aaaggatctt gaaaagagaa tatcagcaaa gcacatttgt taacaaagaa
     5281 tttgcttttc ctcacacaac caaccagcta tctcaacaca ttgttttatc agtagggcta
     5341 ataactgatc aaggtttagc agaaacagga ggaaatggag ttattttttt atttgccata
     5401 cctgaagaaa tgacggctga agttgagaat gaattatttg agctatacga tatcctattt
     5461 gaactcataa aaatgggaat agatgagttt attgaattag caccgacaca agaatcattt
     5521 ttaaaactaa tcaaaagtaa aggagagaga cttcatgaat gatatgtatc tattcatagt
     5581 tttcgcagct tcagcttatg tgctgcaagc agttatcagt tggaagcaac taaagcattt
     5641 caatcaaaca tatagcgaac tgcgaagaaa aggaaaagta gcgattggta aacgacctgg
     5701 gaaaatccgt tcaggcacga ttgttatgct agctatagac gatcatgatc aagttttaga
     5761 agtcagaaaa atgcaagggg ttacatcttt ggcaaaattt cattcattac ctgtctatgc
     5821 gggtgaagat atccattaca tcgatcgtta ccatccctca gttcgagcgg aagatcgttt
     5881 gacacaagca gctttagaaa atgccagaga agtatactta cgaatagagg caggaaatga
     5941 aaaaagtgaa caaccttcat tgccttttat tgctatgctc aataattttc ggatatggaa
     6001 aagtacgatc aaagaaaaaa ggagtgacaa acgatggata tcataacaaa atttgctgag
     6061 ggatttatga ggctgttcca aacaggtgca gaaacattca taagctggat gacaggaatc
     6121 gtaccagttg tcttgatgct gatggttgca atgaatacgc tgattgcttt tttaggtgaa
     6181 gaccgagtaa cgaaagtagc caaattatca gcgaaaaatc cgcttactcg ttaccttgtg
     6241 ttaccgttct tatctgcttt tatgttggga aatcctatgt catttacaat ggctcgtttc
     6301 cttccagaat actacaaacc aagctattat gctgctcaag ctcaattttg tcatacgagc
     6361 aatggtgtat tcccccatat taatcctgga gaattgtttg tatggatggg gattgctcaa
     6421 gggatcgaaa ccttagggct aaattcgatg gagctggcta tccgatactt gcttgtggga
     6481 ctgctgatga actttattgg tggttggata actgatttta ctactggatt tgtatgcaga
     6541 cagcaaggaa tcgttcttag caaaaaagta gagctttcag ttgattagag aaaggagagt
     6601 tttaaatgta tggtgatatt aagatcgtaa gaggaaacgg cgggtatgga ggtcctttga
     6661 ttattaggcc gacaaaagat aaacacaagt tcatctatgt cacaggtgga ggtgagaggc
     6721 caatgatcgt agataaaata gaagaactga caggtatgga gtctatcaat gggttcaaaa
     6781 catcaatacc tgatagtgaa atcgcgcttg caatcgttga ctgtggaggg actcttagat
     6841 gtggaattta tcctaaaaaa gggataccaa ccatcaatat cgttcccaca ggaaaaagtg
     6901 gcccattagc acaatatatc actgaagata tttatgtttc agcagtaggg ttagaacaaa
     6961 tacagccact aatggaaact gatgataaca aagaaacagt aaatactgca tctccagata
     7021 aaaagtacaa atatgatact tctaaaaaga taaccgagca aaaagcagag cagcaaaact
     7081 ggatagcaaa aattggaatg ggcgcgggaa aagtagttgc tacattcaat caatcagcaa
     7141 gagaagcagt agatacgatg atccggacag tgatcccttt tatggctttc gtttcccttt
     7201 tgattggtgt gatacaaggt tctgggatcg gtaatgtatt tgctaacatt atggcacctt
     7261 tagcaggaaa tgcagttgga ttaacaatca ttggctttat ttgttcactg ccatttttat
     7321 ctcctttgct tggtccaggg gcagttatta gccaagtgat tggaacattg atcggtgtag
     7381 agattgggaa aggaaatatt cctccccaat tagcattacc ggcactattt gctatcaata
     7441 ctcaaaatgc atgtgatttt attccagtcg gtctaggttt ggaagaagca aaagctgaga
     7501 cagtagaagt aggtgtacca tcagttcttt attcaagatt tcttaacggc atcccacgag
     7561 ttatcattgc ttgggtagca agttttggat tatatagctg agtttagtct aatggtgata
     7621 agtcaagatg aaatttaaga ttttctttat tgagtaatat gttttgaagc gcacgaaact
     7681 agacccgatc aaaatcaaat ttttttcggg ttgattcaag gatttgcgtc aataacggat
     7741 tgttatttct ctatcacact ccttggtggc gtatagagtt ggtgtttacg tagtagcgta
     7801 tccaccagac gcgtaaattt tcttgcggtt aggacgagtg cacgtttgtg ttgatgcttt
     7861 ggaacctcct tatatttact ttgataaaaa gctttgtatt caggaagata attttttaca
     7921 gagttggcag cttcaactaa gtagtatcta aaatatcgat ttccttgttt tgtcagaggt
     7981 gtattttgcg actgatagtt tccagattgg ttcactttcc aacttaatcc agcgtatttt
     8041 gccaattttg tctgatcttc aaatctttcg atttgaccaa tttcagcgat taaaccggca
     8101 gcatagactt taccaacgcc tggaatactg gttaagcatt gatattctgg aatgacaaca
     8161 attacttgtt cgatggcttt atctaattct ttgatatttt tttcaagtgc tcgtatttct
     8221 cgtacgagaa cacttaaaac aacattaatt gattcttgtg caagagcacc taggcggtaa
     8281 ctcatggtaa ttgcacgttg aattgcttta gcgatttttt ctggttgctt gaaacggcct
     8341 tttccttttt cttgaagtaa atcacagaaa gcttctaaag gaagctcagc aagttcatcc
     8401 aatgtgaaat cttccgtcat gagtgaaatg agggttgcac taaaaagact cgtcgattcg
     8461 tctcgcattt ctcgagcaag cgtattacat ttgtacgtta gattttcaag aaaatgttgc
     8521 tttgtacgaa tcaattgctt aataagttga tagcgagtac gtgttaaacg ctgtaaggcc
     8581 atgtatttct cttctttgat aggggaagtt gtgaatcgtt gaatacgcaa gaaatcagcg
     8641 attcttaatg catcgtttcg atcagtttta ttctcatcaa acatgcgact aaattgtttc
     8701 acccgaaaag gattttcaat cgttacaagt gtgttgagct tctttagttt ctcatcttca
     8761 tgaaaaaaca tggaaggatg gaagctgtac atggacgttg actccatgcc aatcacaa
//
LOCUS       DARBEC010000104         8664 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.108, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000104 DARBEC010000000
VERSION     DARBEC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8664)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8664)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8664
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.108"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1101
                     /locus_tag="SCL80_002447"
     CDS             <1..1101
                     /locus_tag="SCL80_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1108287.1"
                     /translation="KKETIVRFEQYNHMPLVTRLKKQRYTCKNCRTHWTAQSYFVRLS
                     HSIADHVRYKIASLLTEKVSLSFIAKSCQVSLTTVTRTLKEFKSYLPKPSKKILPRVL
                     MVDEFRSHASIEDKMSFICADGETGNLIAVLPTRKLPRLTSYFLGCTNPEEVEFLVTD
                     MNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQALNELRIREMNELRKAGQKSQAEKLKK
                     NWRFLLKNRASINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSAPLKEAYPFFHELV
                     EAFRDKDPDLFFSLLAELPETLDDGFREKLQNLLTYEEGITSAMIYPYSNGKIEAKNT
                     HIKTMKRVSYGFKSFENMRIRIFLINQLINVR"
     gene            complement(1280..1609)
                     /locus_tag="SCL80_002448"
     CDS             complement(1280..1609)
                     /locus_tag="SCL80_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEG1108288.1"
                     /translation="MNTNDIATVYVAFTAAEGGKRRPVYILKNGLDTISFLSITSKYH
                     TKSDKIKLKYVEIRDWKQAGLKKESWIDVGTLNELPKNNKKVKWKKIGELSPSDLQRL
                     EERMEEL"
     gene            complement(1599..1871)
                     /locus_tag="SCL80_002449"
     CDS             complement(1599..1871)
                     /locus_tag="SCL80_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEG1108289.1"
                     /translation="MEKMAKLQISMDEKLKNEAESIIDELGMSPKTAVTVFYKQIVEQ
                     GKIPFSIELSEKSKRIHNIQRLAEKLPVQKLDTDEKIEEWFNEDEY"
     gene            complement(2322..3125)
                     /locus_tag="SCL80_002450"
     CDS             complement(2322..3125)
                     /locus_tag="SCL80_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEG1108290.1"
                     /translation="MRFLHLSDIHFRENYQTNLFTAELFPTIKNPQLALEKLLQELSL
                     TVYDFILLTGDLVHEGGISDYQVLRKMLDQYFPTTPYYFCRGNHDRRSVFFEGMSVES
                     NETGEYIHCTNQNGLRIISLDSAQDTQHEGKISIGQMIQLQEWLGTPAEKGTILLVHH
                     PLSWEDPSIVTEVPEGFAELIAESDILGIFVGHIHQGSSAEFAGKKQFMAESLSFGVD
                     EYPDYSLFTNRTGFTSGSLIDGKIFTYQHYLTPHQIEIGRMKKPITGNH"
     gene            complement(3103..4227)
                     /locus_tag="SCL80_002451"
     CDS             complement(3103..4227)
                     /locus_tag="SCL80_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108291.1"
                     /translation="MGETLELRNIGKKYGEHTILKEIDLAVAAGERIVLLGPSGSGKS
                     TLLRMIAGLEKITSGELFLGTQLANKLPCGEREIAMVFQNYALYPHMTVKENIIFALR
                     ANKIKTADIQHRLEEALKMLGLTPYQDRLPKDLSGGQRQRVALARAVVKRSNYFLLDE
                     PLSNLDVRLRLDARKELVKIHQRYKQTFVYVTHDQIEAMTLADRIVLLNEGRIQMIDT
                     PKNVYTKPKNTFTANFIGSPGMNIMPVTYDQGTLKVASQFLTLNPAWQKQLLQVSGSL
                     YLGIRPEHVTFSKETSDLHGQVKYRELLGQSYAYTVKVDGIEMIVLNEKPTWQIGDAV
                     NLRIEPERMHFFDYQTQENLGYPDYFIEERGNSHALFASL"
     gene            complement(4243..5058)
                     /locus_tag="SCL80_002452"
     CDS             complement(4243..5058)
                     /locus_tag="SCL80_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEG1108292.1"
                     /translation="MKIKRWLPHLLFMFIIIIIIYPLLFIMANSFKPLKDAYQTITSL
                     IPQPFTLENFCYLFENLPIIKITRNTFFIATIITVVKLGVAFFAAYSFTYCRIKGKKI
                     WYFIIIGTMFIPFTVAMIPNYLLIAKLNLIDTTWGVIIPQFADAMGIFLLTQTMREIP
                     VSLLDVAALDNIKQTTVMSKIVFPLTRHAITSTGIWFFITSWNEYVWPVLILRTVDNY
                     TLPLAMQTYISSEGGTNFTVAMAISLITMFVPLVLYAIFQKYIIGTFVSAGIK"
     gene            complement(5048..5920)
                     /locus_tag="SCL80_002453"
     CDS             complement(5048..5920)
                     /locus_tag="SCL80_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006739050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEG1108293.1"
                     /translation="MKRYVGYLYLAPAIILLGVFVFYPLIYTIYLSFFDWNMVAPTKE
                     FVGFANYVKVLTDPVFHKVLKNTLSYTVLFVLLSCVFPYITSFLLGIVIEKGQNLYKP
                     LFFIPAVISLVVAGMVFTWILNPVSGPIAVLLGNFGLRLPMWMNLQGWVIAVIAGITA
                     WKVFGYNFIVIFAAIVGIDQEMIEAAKLDRVPIWRIFFTIVLPMSSSTGLYLLVMTIV
                     QGLQYVFTPIKIITRGGPNNGSANVIYYAYQKAFEMYKVGESSAISVLTLLIFAVFLG
                     VIFKFVEKGVYYEN"
     gene            complement(6005..7294)
                     /locus_tag="SCL80_002454"
     CDS             complement(6005..7294)
                     /locus_tag="SCL80_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEG1108294.1"
                     /translation="MLKKNLVIGVVSVLSVISLAACGSGNTKTTKKSEGRVTIEYWHV
                     NADTLGGKTVNELVDKYNQSQDKVKVVAKYNPDMYKGLMQNLQASVASKEIPDVVQVG
                     WAFKDYFAENFKYTNPQSLVEEVDPDNADYFKDHFLDNIMALSEKDGKQVGIPYSISN
                     PVLYLNQDILKAAGLDENGPKTWEEVQTFSKIIKEKTGKYGVYIQEPADNWAQQAILE
                     SNGASVIKDGKATFASKDGIAAYELYQRMVKDQTALHTTWEQGVQSFIDGNVAMVYTT
                     IAQRDNIQKNSTFTAKAVESPSWEGKKVKLPAGGAMLAVTSQTDKKKEATWDFLKYLY
                     SVESLAKWTEGTGYLPPRKDVADAQNGLKAFLAENQMMIPAIDQMDKMVSWSSFPGDA
                     GLEAEQKLLDMRDKILSGNSVNETMKATQKEINELLK"
     gene            complement(7522..8166)
                     /locus_tag="SCL80_002455"
                     /pseudo
     CDS             complement(7522..8166)
                     /locus_tag="SCL80_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303552.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
BASE COUNT         2689 a         1849 c         1370 g         2756 t
ORIGIN      
        1 aagaaggaaa cgattgtccg ttttgaacaa tacaatcata tgcctttggt tacgcgccta
       61 aaaaagcaac gctatacctg taaaaactgt cgtacccatt ggacggctca aagttatttt
      121 gtccgactca gccattcaat cgcagatcat gttcgatata aaattgcttc tttactgact
      181 gaaaaagtat ctttgtcttt tattgcgaaa agttgtcagg tatctttgac taccgttact
      241 cgtacattga aagagtttaa aagctattta ccaaagcctt ctaagaagat tctccccaga
      301 gtattgatgg ttgatgaatt tcgttcgcac gcttccatag aagataaaat gagctttatt
      361 tgcgcagatg gtgaaacagg aaacttaatt gctgtcttgc ctacgcgtaa attacctcga
      421 ttaacaagct atttcttagg ctgcaccaat ccagaagaag tagaattctt agtcacagac
      481 atgaacgccg cttacttcca gcttaccaaa cgtgttctgc caaatgcgaa agtagtgatt
      541 gatcggtttc atattgtcaa acacatgaat caggcactca atgagttgcg tatccgtgaa
      601 atgaatgagc ttcgtaaagc aggacagaaa agtcaggcag aaaaactgaa aaagaactgg
      661 cgctttctgc taaaaaatcg tgcaagcatc aaccattatg agtacaaaac atggaaaagt
      721 ttccgagcac caaaataccc atttctcact gaagcaatga tgattgatcg attgcttgag
      781 ttttctgcgc ctctaaagga ggcgtatccc ttttttcatg aattagtcga agcctttcga
      841 gacaaagacc ctgacttatt tttctcctta ttggcagaac ttcccgaaac gttagatgac
      901 ggctttcggg agaagcttca aaaccttctg acctatgaag aaggtatcac cagcgcaatg
      961 atctatcctt attccaatgg aaaaattgaa gcgaagaata cccacataaa gacaatgaaa
     1021 cgagtatcct acggatttaa atcattcgag aacatgagaa ttagaatctt tttgatcaat
     1081 caattaatca atgtaagata acaaaaaatc tgaaccagaa tgtggttcat tctgacccgg
     1141 atttgatttt gcacaactca tcagtcctta ttgacaaaga gccaaaaatc tgagccagaa
     1201 tgaggttcat tctgacccag atttgatttt gtacaaccca tcagtcctta ttgacaaaga
     1261 gcccttcttt ttagtattat tataattctt ccatgcgttc ttccagtcgc tgtaaatcgc
     1321 ttggacttaa ttccccaatt tttttccatt tgaccttttt gttgtttttg ggtaattcgt
     1381 ttagagttcc tacgtcaatc caagattctt tttttagtcc tgcctgtttc caatccctta
     1441 tttcaacgta tttcagcttt attttatcgg atttagtatg atattttgaa gtaattgaca
     1501 gaaacgaaat tgtgtccagt ccatttttta gaatatagac aggccggcgt ttaccgccct
     1561 ctgcagctgt gaaagcaacg tagacggttg ctatatcatt agtattcatc ttcattgaac
     1621 cattcttcaa ttttttcgtc agtgtctagt ttttggacag gcaatttttc cgctaatctt
     1681 tgaatattat ggattcgttt acttttttcc gacaattcaa tagaaaatgg gatttttcct
     1741 tgctcaacaa tttgcttata aaaaacggtg acggctgttt tggggctcat gcctagttca
     1801 tcaataatgc tttctgcttc atttttcaat ttttcgtcca tagaaatttg taacttagcc
     1861 attttctcca cctctttctg acaaacacat tatatcacgg aaagagttca aatggaatta
     1921 atacaactac ttttgaactc cttgaaatgc agtgtattta tctaaatata aggaaaggat
     1981 atagtgctaa atggccttga tagatagaaa agtttcaaaa tccgccacag cgataattga
     2041 agcgaacact caacgacaca cagaacagga aaagactcct aaaagaacca aattaactag
     2101 gacgtgtctg aaaactccac aaagatgtgg tatgctctgg taaaaaacga atggcacaac
     2161 cacgaagacc gatatggttc ttggaaaact ttctccaccc tttttgtaat tggagggata
     2221 gtggtttatt gatagcgatt ttccaagttc ttcaagtgga accagacatt gagcaccacg
     2281 gctaaaaagt gaatgaagtc tttattttta atcaataatt tttaatgatt accagtaatt
     2341 ggttttttca tgcgccctat ttctatttga tgtggagtta aataatgctg ataagtaaaa
     2401 atttttccgt caattaaact gccactagtg aatcctgttc tattggtaaa aagagaatag
     2461 tctgggtatt catctacacc aaatgataaa gattcagcca taaactgttt tttaccagca
     2521 aattcagcac tgctaccttg atggatatgt cctacaaaaa tacctaaaat atcactttcg
     2581 gcaatcaatt cagcaaaacc ttccggaact tcagttacaa tagatggatc ttcccacgac
     2641 aatggatgat gaaccaataa aatcgttccc ttttcagcag gtgtacccaa ccactcttgt
     2701 aattgaatca tctgtcctat cgatattttt ccttcgtgct gggtatcttg agcagaatcc
     2761 aaggaaataa ttcgtaaccc attttgattc gtacaatgga tatactctcc cgtttcattg
     2821 ctttcaacac tcattccttc aaagaaaaca ctgcgacgat catgattccc acgacaaaaa
     2881 tagtaaggtg tggttgggaa atattgatcc aacattttcc gtaagacttg ataatcagaa
     2941 atcccccctt catgtactaa atcacctgtt aatagaataa aatcgtacac tgttaacgag
     3001 agttcttgta acaatttttc caatgccaac tgtggatttt taattgttgg aaatagttca
     3061 gcagtaaaca agttagtttg atagttttct ctaaaatgga tatcagagag atgcaaaaag
     3121 cgcatggcta ttccctcttt cttcaataaa ataatcagga tatcctaaat tttcttgcgt
     3181 ctgataatca aaaaaatgca ttctttcagg ttctatgcgg agattgactg catcaccaat
     3241 ctgccaagta ggtttttcat taaggacaat catttctata ccatcgactt taacggtgta
     3301 cgcataactt tgacctaata gctcacgata cttcacttga ccgtgtaaat cgcttgtttc
     3361 ttttgaaaag gttacgtgtt ctggtcttat gcctaaatac aatgagccac taacctgaag
     3421 aagttgtttt tgccaagctg gattcaatgt taaaaattga ctggctacct ttaatgttcc
     3481 ttgatcataa gtaactggca taatattcat gcctggcgaa ccaataaaat ttgcagtaaa
     3541 agtattttta ggtttcgtat aaacattttt cggcgtatca atcatctgaa ttctaccttc
     3601 attcaataaa acaatccggt cagctaacgt catcgcttca atttgatcat gagtgacgta
     3661 gacaaaagtt tgtttataac gttgatgaat ttttaccaat tcttttcgtg catccaatcg
     3721 taaacgcaca tccaaatttg ataacggttc atctaataaa aaataattac tccgtttaac
     3781 caccgcacga gccaatgcta cacgctgccg ttgccctcct gacaaatctt taggaagtcg
     3841 atcttggtat ggcgttaaac ctaacatctt caaagcttct tccaaacggt gctgaatgtc
     3901 agctgtctta attttattcg cccttaatgc aaaaataata ttctccttta cagtcatgtg
     3961 aggatataaa gcataatttt gaaaaaccat tgcgatctct cgttctccac aaggtagctt
     4021 atttgctagt tgtgtaccta aaaaaagctc tccactcgta attttttcca aaccagcaat
     4081 cattctcagc aaagtagatt ttcctgaacc agatggtccc agtaacacaa ttcgttctcc
     4141 agcggccaca gctaaatcaa tttcttttaa aatggtatgt tctccatatt tttttccgat
     4201 atttcttaat tcaagcgttt ctcccatgat ttgtcctcca atttatttaa tacctgcaga
     4261 aacaaaagtc ccgataatat atttttgaaa aattgcatac aagactaacg gtacaaacat
     4321 tgtaattaaa ctaatcgcca ttgctacggt aaaatttgtc ccaccttcag aactaatata
     4381 ggtctgcatc gccaacggca aagtataatt atctactgtc cgtaaaatta ataccggcca
     4441 aacatactca ttccaagagg taataaaaaa ccaaataccg gtagaagtaa tcgcatgtct
     4501 agtaagagga aaaacaattt tactcatgac ggttgtttgt ttgatgttat ccaatgccgc
     4561 cacatctaga aggctcactg gaatttcccg cattgtttgc gtcaataaaa aaatgcccat
     4621 cgcatcagca aattgtggta ttatcacacc ccaagtagta tcaattaaat taagttttgc
     4681 aatcaacaaa taattaggaa tcatcgcaac agtaaaagga atgaacatcg taccaatgat
     4741 gataaagtac cagattttct tccctttgat tcggcaataa gtaaaactat atgccgcaaa
     4801 aaaagctacg cctaacttaa caaccgttat aattgtagca ataaaaaaag tatttctcgt
     4861 aattttgata attggaagat tttcgaacaa atagcaaaaa ttttccaagg taaacggctg
     4921 tggtatcaaa ctagtaatcg tctgataggc atctttcaat ggcttaaaag aattggccat
     4981 aataaacaat aatggataga taataatgat tatgatgaac ataaataaga gatgtggcaa
     5041 ccagcgttta attttcatag taaaccccct tttccacaaa tttaaatatt acccctaaga
     5101 aaacagcaaa tattaataaa gttaaaactg aaattgctga agattctccg actttataca
     5161 tttcaaaagc cttttgataa gcataataga tcacgtttgc actaccatta ttcggtccac
     5221 cacgagtgat gatcttaatc ggagtaaaca catactgcag accttgaaca atggtcatca
     5281 ccaataaata tagtcctgtc gaactactca taggcaacac aattgtaaaa aaaatgcgcc
     5341 agatgggtac acgatccaac ttagctgctt cgatcatctc ttgatcaatt cccacaattg
     5401 ctgcaaaaat gacaatgaaa ttataaccaa aaactttcca agcagtaata cctgcaatca
     5461 cagcaattac ccaaccttgt aaattcatcc acataggcag tcttaaacca aaatttccca
     5521 acaagacagc aattggtccc gataccggat ttaatatcca tgtaaaaacc atccccgcca
     5581 ctaccaaaga aatcacagct ggaataaaaa ataatggttt atacaaattc tgcccttttt
     5641 ctataactat tcctaataaa aaggatgtaa tatatgggaa cacacagctc agcaagacaa
     5701 atagaacggt ataggacaaa gtattcttca acactttatg aaagactgga tcagtcagca
     5761 cctttacata gttggcaaag ccaacaaatt ctttagtggg ggccaccata ttccagtcaa
     5821 aaaaactcaa atagatcgta taaatcaatg gataaaaaac aaacacgccc aacaaaataa
     5881 ttgctggcgc cagatacaaa taacctacat agcgcttcat ttccgacagc ctcctcatcc
     5941 ttatgtcaaa agcagacgac cttgtttttc ctttcttaca ggcgctgtcg tctgcttgct
     6001 ttgcttattt gagtaactca ttaatttcct tttgagtagc tttcatcgtt tcattgacgg
     6061 aattacctga taaaatttta tcccgcatat ccaacaattt ttgttcagct tctaatccag
     6121 catctccagg aaatgaactc caactgacca tcttatccat ctgatcaatt gctggtatca
     6181 tcatctgatt ttcagctaag aaagctttta atccattttg ggcgtctgca acatctttac
     6241 gtggtggtaa ataaccagta ccttctgtcc atttagctaa agattcaaca ctataaagat
     6301 attttaagaa atcccaagtt gcttcctttt tcttatccgt ttgtgatgta acagctaaca
     6361 ttgcgccacc agcaggtagt ttaacttttt tcccttccca acttggtgac tcaactgctt
     6421 tagcagtaaa agtactattt ttttgaatgt tgtccctttg agcaatagtg gtatagacca
     6481 tcgcgacgtt accatcaata aagctttgca caccttgttc ccacgtagtg tgtaatgccg
     6541 tctggtcttt taccatcctt tgatataatt cataagcagc gataccatct ttagaagcaa
     6601 aggtagcttt gccatctttt attacacttg ccccattact ttctaaaatt gcttgttgtg
     6661 cccaattatc agcaggttct tgaatataca cgccatactt tccagttttt tcttttatta
     6721 tcttagaaaa agtttgaact tcttcccaag tttttggacc attttcatcc aagcccgctg
     6781 cttttaatat atcttgattt aaatataaaa ccggattact aatggagtaa ggaataccca
     6841 cttgtttacc atctttttca gacagtgcca tgatattgtc taaaaaatga tctttaaaat
     6901 aatcagcatt atccggatcc acttcttcta ctaaagattg aggattagta tatttgaaat
     6961 tttctgcaaa ataatcttta aaagcccatc ccacttgtac tacgtccggt atttctttgg
     7021 aggcaacaga agcttgtaaa ttttgcatca agcctttata catatcagga ttatattttg
     7081 ctacaacctt cactttgtct tgagattgat tgtatttatc tactaattca ttcactgttt
     7141 ttccacctag ggtatccgca ttaacatgcc aatactctat cgttacacga ccctccgatt
     7201 tttttgttgt cttggtattc ccactaccac aagctgccaa agagatgaca cttaaaacag
     7261 acacaacccc tataacaaga tttttcttta acataacatt aacttcctcc taatttcttt
     7321 atggccattt ttctaatcaa gaaatattat gaactaccaa tgtaaatttc tagttaattt
     7381 tctgtaaata attcgaaatt tgtgcatttc ggggtacagg gtcactggac atatccatca
     7441 agctaagaat aaagccccca tatggtcatg tatcccatct ccaaatctct taactcctct
     7501 gttttgatta taaaatagtt tattctgtgc tcctttcttc cataaatagg cccatgcaca
     7561 acgtgaggtt atattataaa caccacagcg cgcaaggtac tcaatcgttt ctagttcatt
     7621 gactgtccat acagaaaatg tcaatcctag ctgctgcccc acttcacggg ccaacttagt
     7681 taaccatcct tcatgaatat tgacgatttg aacgctttga taggctaatt tctgtaaggt
     7741 atcgatcaaa tctgcttcgg taatatcccc aagttttttc ccttcgaact gattttcaat
     7801 attataaaat atatgctttc cccaaatcgc agcccaagat aattgtacat agccactaag
     7861 aacaatttgt ggccagattt gataggtttg tgccagcgaa aaaacggcca tttccaatcc
     7921 ctcttctttt aaatcacagt taatggtcat ggttgccttc tcattggcca ctgcttgaaa
     7981 cacctgctca agggttaccg aatctgggac aacttcttac ctgtcatggg ctagtattag
     8041 cgtaccatct gtttgctgac gcacatctac ttcaaaacaa agctggcgat ccaaacaaaa
     8101 gcgaagaaat gcccagctat tctcctccgt attgtcacta ccagagtgtg cagtgatcat
     8161 ggtcattttg acccctcctt taattccgtg attttctccc acacgtccac tataccgatg
     8221 aagcgttaat ttcttgtaaa ttccctgcgt tttttcagta aactagttgt aaatcttgca
     8281 tgctccttcc tacgaactca tgaatcgtcg attttgccct tctagctagt ttcaccaatt
     8341 aagtagttct acattccccg cttttaacct actacacctt tttttgtttt cagctattcc
     8401 ttcttcttgc tgaaagtcct tgaggttctt tcttacagga taccatcaag gtgttcaatg
     8461 gcttattccc attgaatgac cgagacttac tatcagattt ctgctcgttg aaccaagtct
     8521 ttgatacagg atttcttccg tcatcttctc acaaatacca ccttgcgttt ctctagcgat
     8581 tggtcgatgt gcaccctccc agtggactgt cactacccct ttttgctatg cctagcccac
     8641 aaaaaaagct gtcctcacag acag
//
LOCUS       DARBEC010000105         8586 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.109, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000105 DARBEC010000000
VERSION     DARBEC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8586
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.109"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(45..1511)
                     /locus_tag="SCL80_002456"
     CDS             complement(45..1511)
                     /locus_tag="SCL80_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEG1108295.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
     gene            1898..2494
                     /locus_tag="SCL80_002457"
     CDS             1898..2494
                     /locus_tag="SCL80_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1108296.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAGEKEYMNDADRFRVFG"
     gene            complement(2552..3034)
                     /locus_tag="SCL80_002458"
     CDS             complement(2552..3034)
                     /locus_tag="SCL80_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108297.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(3140..3337)
                     /locus_tag="SCL80_002459"
     CDS             complement(3140..3337)
                     /locus_tag="SCL80_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108298.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            3632..4390
                     /locus_tag="SCL80_002460"
     CDS             3632..4390
                     /locus_tag="SCL80_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEG1108299.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            4450..4878
                     /locus_tag="SCL80_002461"
     CDS             4450..4878
                     /locus_tag="SCL80_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108300.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            4924..4996
                     /locus_tag="SCL80_002462"
     tRNA            4924..4996
                     /locus_tag="SCL80_002462"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4957..4959,aa:Gln,seq:ctg)
     gene            complement(5146..5517)
                     /locus_tag="SCL80_002463"
     CDS             complement(5146..5517)
                     /locus_tag="SCL80_002463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEG1108301.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            5732..5917
                     /locus_tag="SCL80_002464"
     CDS             5732..5917
                     /locus_tag="SCL80_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108302.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            complement(6000..6188)
                     /locus_tag="SCL80_002465"
     CDS             complement(6000..6188)
                     /locus_tag="SCL80_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108303.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(6212..6475)
                     /locus_tag="SCL80_002466"
     CDS             complement(6212..6475)
                     /locus_tag="SCL80_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108304.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            6633..6890
                     /locus_tag="SCL80_002467"
     CDS             6633..6890
                     /locus_tag="SCL80_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEG1108305.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            complement(6961..7152)
                     /locus_tag="SCL80_002468"
     CDS             complement(6961..7152)
                     /locus_tag="SCL80_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108306.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(7293..7613)
                     /locus_tag="SCL80_002469"
     CDS             complement(7293..7613)
                     /locus_tag="SCL80_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108307.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            complement(7636..8070)
                     /locus_tag="SCL80_002470"
     CDS             complement(7636..8070)
                     /locus_tag="SCL80_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEG1108308.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            8405..>8586
                     /locus_tag="SCL80_002471"
     CDS             8405..>8586
                     /locus_tag="SCL80_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEG1108309.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNS"
BASE COUNT         2718 a         1694 c         1362 g         2812 t
ORIGIN      
        1 aggaactgct gcaaataagc aattcctgtt tttatgttca ttgattaatg aatagagccc
       61 attagttttt tgattggttc tttcacagta aatagcaaga taccaccgac gattgctact
      121 gcaccgataa taccgaagta agcagattct gttcccggtg tgtagaagcg tgcaatttgt
      181 gcattgattg cttgtgaaga agcatcagct aagaaccaaa tagcgattgt ttgtgattca
      241 aatgctcgtg gtgctaattt tgttgtcaca gacaaaccaa ctggtgaaag acacaattca
      301 gcaataacca cgatgaagaa acttcccaac aaccacaatg gacttacttt tccacttgta
      361 ccataaagca tacccggcaa catcaatagc aagtacgata atcctgcaaa taaaagtcca
      421 agagagaatt tcactactgt agatggttga cgttcaccaa gtttcgtcca catcgttacg
      481 aatactggtg tcagaataaa gatgaaaatt ggattcaatg attgatacca gcttgcttgg
      541 atcgttacac caaatagcgt agattgtgta cgttcactag caaataaagc taagatagat
      601 gaaccttgtt cttgtaatga ccagaaaatg attgctgcaa ggaataatgg gatataagcc
      661 catactcttg gtttttcttc agcagttaca tcttttgaag tcagcatttt gataaagtaa
      721 tacaacggta agataatccc caatacactg attgtattga caaaaaagtc aattgtcaga
      781 tgaccagtta gttgtgcccc gccgaatact gcagcgataa caacaagtgc gatgatcaag
      841 ttacggatga atcctttacg ttcttcagtt gtcaaaggat tcggcggtgt tgtacccact
      901 ccgtctaatg tcttgcgtcc ttggatgatg tattgtaata atccaaagaa catcccaata
      961 gcagctaaag agaaccctaa atgatagtta tattcttgac cgattgtacc aacgattagt
     1021 ggtgctaata atgcccctaa gttgacaccc atgtaaaaaa tcgaaaatcc tgcatctctt
     1081 cgcaagtcag tctttgaata aagatgtccg accatgccag aaacatttgg tttcaaaaag
     1141 gcggttccga taataatcaa tgccatcgaa acaaacaatg tacttacacc aaatggtaaa
     1201 gccaagacga tgtggccaag cataatgaaa actccaccga agaatacagt gcgttttgct
     1261 cctaacactc ggtcagatac ccatccgccg acgatactag acatgtagat caaagatccg
     1321 tagatcgaca tgatagctaa tgctgttgct cgtggtaatc caagtccccc gttggcaact
     1381 gtatcgtaca tataatagat aagtaatgca cgcataccgt aataactaaa tcgttcccac
     1441 atctcagtga agaacaaagt tgacaatccc ttcggctgcc cgaagaagcc cttgtcttgc
     1501 tgtttttcca taatctatac cccaattctt taaattttca gaaaatatta tacatcttta
     1561 ccgaaaaatt taactaaaaa acttatttaa gaaatataaa ctaattttct ataaaagcaa
     1621 gtaattttta atggcaatat cataaaaaaa agaagataga gatttaataa ttatatatca
     1681 gctgtttttt tcaaataagc aaaaattcct aaaacacaac aatgaaagcg ataactaaga
     1741 aaatccttac ataacgattt ttcttattaa aattgttttt tgaacttcgc ttattttttg
     1801 tgagcatatc tgtttactta ctttttgtaa ggtgctatac ttcatttcgt aatcaattaa
     1861 aaaaatgcaa aacgcaaatc aaaggagaaa ttttattatg acaactttta ctgacacttt
     1921 gaaaaaccgc cgttcaatct atcatctagg acgtaatgtt tcactttcta atgaagaatt
     1981 aacaactttg atcaaagaag caattaaaga aagcccaact gctttcaatg ctcaatcaac
     2041 acgcgcagtt atccttttcg gagatgcaca tgaaaaactt tgggaaatca ctgaagaagc
     2101 acttcgccca ttgactccag cagaagcttt cccaaataca caaaacaaat tagctggttt
     2161 caaaaatggt tatggtacag tattattctt caaagatact gacgtggtaa aaggtctaca
     2221 agaacaattt gaattatatg cagacaactt cccagattgg tcagaacaat caaacggaat
     2281 cgcaacagct aatacttggg tagcattagt tgataaagga ttgggcgcaa acttgcaaca
     2341 ttacaaccca gttattgacg aagcagttgc aaaagaatgg aatatccctt caaactggaa
     2401 actacgttcg caattagtat tcggttcacc agaaacaccg gctggggaaa aagaatacat
     2461 gaacgatgcc gatcgcttcc gcgtatttgg ataattttat ttgaaataaa aaacagaggt
     2521 tgtgacaaac atcttgtcgc aacctctgtt tttattctgg atcaatcata atatcaatct
     2581 ctgcctcatc gtttaagcgt tgggacaaaa atggaactaa ttcttctaat gatgcagaaa
     2641 atttcagcat cggtcctcgc catccgtttc ggaaaaaatt catttttacg attttagaac
     2701 cttttggctg atataataca attttattga tctcagaaaa aggaacacct tttttgtcaa
     2761 agctattcaa aacaagtcct tcttctgtga ttcctctact gtccaacaaa aaactaataa
     2821 aaatcattgc tgacaagatc ccttttgcca attcatctgc tgtaggatga ctgatgagtg
     2881 tataccaaat gactagtgac actaagaagg gtatcaccaa gtagacccat cgatttcgca
     2941 cagtccgata aataatttct tttcgaacca gccaaagaaa taaaaacatc acaacaacga
     3001 ataaaaacaa tagaaccgta aagaatgtta ccatgacaac tcgcctcgct ttttcttctc
     3061 cagaaaatag gaaaagaata ctgatttacc ttctcaaaga aattggactt gcagcacgcc
     3121 gcacgtccaa ttgttttctt tattcaaatg tatcatattt tgcaacaacc atgcttgctt
     3181 tttcatctaa taattcttgg tcctctgcag cacattgggc aactttgacc acggcgcagt
     3241 ttgtcatttt tgatacaact tcaccaatga ccgtttttgt aaaaccaatt ggtttgcatg
     3301 catattgttc tcctaaatta atcatttctt ccgtcatcaa aatcctccta aactgctgga
     3361 acttttctcc acattaccac tgctatttct ttggagattt aactcattga tttttattta
     3421 cagttaaaaa tcaaccttac aggcattata gcgtactgtt ttcgttttca caattaaaac
     3481 agctgttgta tttagaagaa tttttttcaa aaaattaaaa catattcctt aaaaactaat
     3541 tatttcctac atattaaaat ataatacttc catttccaat aaaaatacat ttataatagg
     3601 aaagataaac agctgaaaag ggagatttca aatgaagaaa aaaacgttca aaaaactatt
     3661 gtttctaggg ggcacagctg ctcttttatt aagcggtaca agttattacc tacatatcca
     3721 ttctccagaa catacaatga acactgcaca aaaaagttca acacaaaaaa cgaaaaagac
     3781 aaaagagaaa gcagaagttt tactagatgt tccattagag agccagtttg attctccttc
     3841 tttagaaaat ggctgtgaag tcacttcgct ttctatgctt ttatcttttt atggttatga
     3901 aacaactaaa aaccagttag ctgagcaatt ggattacgtc cctgttttca atgcagatgg
     3961 gactcatggc gatcctaatg aaggatttgt tggtgatatc agtggcggag actgggctat
     4021 ggtcgtctat gttcccccag ttgcctccct tgctcagcaa atcgtccaaa cggattatca
     4081 cacttttcca aaaaccgatg ctcaactcga tgatatcaaa aaagcactat ctaaaggaaa
     4141 tcctgtctgg acttctgtga ctatcaattt tgaagtccca gatgaaactg attttatgac
     4201 ttggaccaca aataatggag aagtaagagt cactcctctt gttcatgctt gtgtagtcac
     4261 tggatatgac aaagaaaata tttatgtcaa cgatccctat ggtgtaaaaa atcgagcagt
     4321 accgatcaac gatttttcgg cgattttcac tgcaatgggt ggacagatgc taactttgga
     4381 gaaaagctag taattcaaac aaaaatccct tatactcaag ataccaaacc tttgaaggga
     4441 gcgtagataa tggaaaaatt cacacataaa aaaatggatc caaatgaaat tccgattatt
     4501 tttgtccgtg actgcaaggg aaatgttcaa ggtaaagtct ctattaatga gtggaacgag
     4561 cgtcgcagac ctgctaccct caatgaactg gaaatcaaat tgtatcgtca atctcttgtt
     4621 tattacgcag atcaagagta cgagaaagcc accgatttat tgaaatttct aattgctcga
     4681 acagagtata ctcgctttga atatattgaa cgtttagcaa acatctatca tattatgaat
     4741 gaacctgtca aagaatatca gttattggac accgtcttgt cagtagcaga actgattgcc
     4801 cttcctgctg gattagaaaa gaaactagta cgccgccttt tacgagtaaa gcaacaacta
     4861 tccgatcaag aaaaataact tgcgtttttt taggaaagtg ctatgattat tttgttgaat
     4921 aaatgggatg tagccaaact ggtaaggcac ttgactctga atcaagcaat tgtaggttcg
     4981 aaccctgcca tcccaatttg atgtgcttgc tgaaaaaatg aaccccctgt aaaagaacaa
     5041 tctttttgca gggggattat tgtattattt ttactacttg tatttagttt acataattaa
     5101 attatgaata ataaaatcgg gcgtattctt aatacaaaaa ctgatttacc caataattgc
     5161 taatgctttc tccggaactt tttctctagg aacttcctca tacgtttcta tgtgtgctcc
     5221 tttattgcta attttcaaat aatgattttc ttttaacaca gaaagaccat taaattcaac
     5281 cgttcgttct ttcccattgg catcagcagc tttctgcaca taccttgctg taccatattc
     5341 atttacttcc tctggttttt gtgtctttac atagacctct cctttttcaa ctaatggatt
     5401 tagctgatca agtataccag ggatttctcc ataacttcct tcagtataat atttcagtcc
     5461 aaatagcaaa ataccactaa tcgccgccag tccaatcaaa tatttaatca ctttcatctt
     5521 tctacctcct tgttatatat tcagtatatg ggtcagttaa agaaattcca tccgtcccaa
     5581 gttggagatt tttctcctac tttagaagcg agaatcgagt gtttttttag taaaaatgat
     5641 cgttatttga tattattgac gtgtggataa atcagttttt ctcgttttgt ccatgtaaaa
     5701 aatcatgttg atagcaaagg atgtgttcaa aatgaatcaa gaagaattat ttcaaaaagt
     5761 caaggaaatg attaagaacg gcaatttcga tggcgcaaaa cgatttatcg aagaacataa
     5821 agaacaatta ggaccttata aagagaaagc ccaaaatctt ttaaaagatg taaatatcga
     5881 cagtgtcaaa aacaaattta aaaatctatt caaataagta aaactttcac ttttgtaaaa
     5941 tcaaaggagg acaatgctgc tgcatcgtcc tcctttggtt cttttctgaa tacttcatac
     6001 taaaataaac gtgtcggatc gtaatgataa gtcacattgc cgttcagtga ggcttttccc
     6061 accacatatt tacgattctc caacttcagc cacgcttctc gaaaagccat taattcttct
     6121 ctttttacat caatcttttc gatttttcca tcgatcaaat cttgaataag ttgttcatat
     6181 ggattcattt ttttcctcca tctgctgtca atcaataagt tgtaaaaata ggttcatatg
     6241 cttttttggt atctagatct tctctgactt tgactgtcgt ataaataatt ttttctactg
     6301 ggatactata ataacttcct tcaccattat tgaatcggtg aacgtctttc tttgctaaaa
     6361 taccatttat tttattcgtc agtacacgtt gttcgacact gttacttaca acatagtaaa
     6421 tcacccgaat attattgatc aaatgcaaat cgatttctaa cactacatga tccatgaaaa
     6481 tcccactcct tctttcactt tgattatagc aagtttctcc tctaatttca aaatcgatac
     6541 agattttacc ggataaatcc acaaaactgc ttgcgccatt tcgcacaaag tgttattttg
     6601 tatcatggac tttatttatc ggaggcaata gtatgatcgc gatcggtcaa tttgtttttt
     6661 atattccttt ttttattatg ataagcattc ttttctatta cataaaatgg acaaaaaaga
     6721 agttttcagt tttactggcc tctttacctg ctgtatattt tacttatcaa attttttctt
     6781 ttaggcattg ggaaacaacg tctgtattaa taacacacat catcgaattg acgctttcag
     6841 taatttttct tattatttgg atctattttt tatataagaa tcagaattga atccctcgca
     6901 tgtttaaaaa gaaaacgatt aaagatgcag caaatttctg ccgatcttta atcgtttttt
     6961 ctattctgtt ataccaaaaa aatgtttgaa tcggtgaagg atcgttcctt gttttaattc
     7021 tataaattct tgtgcgatcg ggtacttttc ttccaaattt tctttgttct tttcgatcag
     7081 gcttttcgct tcttcatatt ttttatctgc acatagtgct tttacttcct gtaatacttt
     7141 tcctgcttcc atttgcctca ccctcttgcc attttattgc tttaagtttt atttaagtta
     7201 tacgcattat tctagtttca tcccaaataa ccttttttca tttttactcc tcgaactaat
     7261 cagttttcta aaggctgatt ggttcgtctt ttttattttt cctccatttc attctgctct
     7321 tctgttaatt tatctaatgc tttgattgat gctgcggctc ttgcataatc atagctgtat
     7381 aattccataa atttatcagg cagaacaaac cacgctggat gataaccgtt agctgtgaca
     7441 gggaatcctt tatcttgttg gttatctcca tcccatctca gagcaacacc tttttttcct
     7501 ttccacttta ctaaagcgat cgagtatccc ttctttgttt gtgtgccgtc ttcaataatt
     7561 gctaaaattt ctattttttc ttttggcgca cggacttctt gaggacgaac cattttctat
     7621 tcctccctta tctcattact tcacgaccaa cacactgcaa ggggcatgtg atacgacata
     7681 agaagcggtc gatccaacta atgcttgttg tatagcacct tttcctgtcg ctcccataac
     7741 gatcaaatca atattttctt gtccagggat gacattggca atcaaatttc ttggatctcc
     7801 agtttcaact attgcgtgga tctcagcaat gccgtactct ttggcatcat tgattttttt
     7861 cagcatggct acttctgttc tttgtttttc atctgcatag atttttgaaa aggcataggc
     7921 actatgggtc agctcaactt tgttgatgat tgacacgata aataagtggg cttcattgtc
     7981 ctttgctaag tcaagggctt cagcgaacgc tttgatcgat tgtcttgatc cgtcaactgc
     8041 taccatgatg ttctggtatc ttttactcat tggaaccgcc tcctttttct tttattgtaa
     8101 catctcaagc aaaaatacaa aaaatatacg caatatgtaa ataaaatgat gaacaaaagc
     8161 aatttctttc aagttattta tacatttctt ttctcatcag aacacctgtt ctaatcccat
     8221 tatttctgca ttatgtgaaa ttcctcatca tttttcacat cgagcgaaca tcattctgtt
     8281 acaaaaaacg tggatttgtc ataaattgtc atgcttcttt tacctatgaa atattttcac
     8341 gtgttaaagt attcctcgtt gaacacaaca aataaaactt tttaacgaat aaaggagatt
     8401 tttcatgaca tcacttaaaa ctttactttt tggaacaact ttggctgccg gcgctgcatt
     8461 cttcatggga acgactgctc atgcggacga agcttatacc gttcaatcag gtgatactct
     8521 atcaacgatt tctcaaaaat acgttggtga taactccttg atcaatgcca ttgcagaatc
     8581 aaattc
//
LOCUS       DARBEC010000106         8494 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.110, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000106 DARBEC010000000
VERSION     DARBEC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8494)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8494)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8494
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.110"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..173)
                     /locus_tag="SCL80_002472"
     CDS             complement(<1..173)
                     /locus_tag="SCL80_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108310.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="SCL80_002473"
     CDS             complement(593..1714)
                     /locus_tag="SCL80_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEG1108311.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="SCL80_002474"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="SCL80_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEG1108312.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="SCL80_002475"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="SCL80_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEG1108313.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="SCL80_002476"
     CDS             complement(4304..5215)
                     /locus_tag="SCL80_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108314.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="SCL80_002477"
     CDS             complement(5232..6239)
                     /locus_tag="SCL80_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEG1108315.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="SCL80_002478"
     CDS             complement(6236..8365)
                     /locus_tag="SCL80_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1108316.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8494)
                     /locus_tag="SCL80_002479"
     CDS             complement(8370..>8494)
                     /locus_tag="SCL80_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1108317.1"
                     /translation="NGQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2681 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg tttt
//
LOCUS       DARBEC010000107         8248 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.111, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000107 DARBEC010000000
VERSION     DARBEC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8248
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.111"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            476..1339
                     /locus_tag="SCL80_002480"
     CDS             476..1339
                     /locus_tag="SCL80_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEG1108318.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1468..3090)
                     /locus_tag="SCL80_002481"
     CDS             complement(1468..3090)
                     /locus_tag="SCL80_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEG1108319.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3339..3860
                     /locus_tag="SCL80_002482"
     CDS             3339..3860
                     /locus_tag="SCL80_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1108320.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCL80_002483"
     CDS             complement(4046..5776)
                     /gene="spxB"
                     /locus_tag="SCL80_002483"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEG1108321.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5941..6759
                     /locus_tag="SCL80_002484"
     CDS             5941..6759
                     /locus_tag="SCL80_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEG1108322.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKRHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6862..7059)
                     /locus_tag="SCL80_002485"
     CDS             complement(6862..7059)
                     /locus_tag="SCL80_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108323.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            7228..7806
                     /locus_tag="SCL80_002486"
     CDS             7228..7806
                     /locus_tag="SCL80_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEG1108324.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7901..8038
                     /locus_tag="SCL80_002487"
     CDS             7901..8038
                     /locus_tag="SCL80_002487"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108325.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
BASE COUNT         2475 a         1483 c         1627 g         2663 t
ORIGIN      
        1 ccactaaacg gtgttctcct gccaactcct cggtcacaag tcacaatatt caacaaacat
       61 gagaagctgt tttttgaaat tgttccttgt gactcggagc taaacggcag gctcacaacc
      121 tcttggtaaa cggtgttcaa tcagctgacc ctcggtatta gctcaaaaaa cggcaaaata
      181 tgaggaacta ttttttccgt ttttttctct aatactcagg tcaaaacgct gatttcacaa
      241 cctctgattc acggtgttcc agaagtagct tctacggaaa taagccgaaa tttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttat tttgattaga acttgcgaaa ataaaccggt taaagtgtgt ctagcgcaag
      421 agatttgcta taatagacga taagggtaaa atgtaacgag aaggggttga aaaatgtgta
      481 tcaaattgta acagattctt gttgtgatct gccataccat gtattagcaa aagaaaaagt
      541 tgatttttta tcaatgaatc tccaattgaa tgatcaagag ttagtagatg acctcggaaa
      601 gacattcaat tatgaccgct acatgaatca gctgaaaaat ggtgcgatgc ctacgacatc
      661 acaaatcaat gttggccgat atttagaatt tttccgttct tatgcggaaa aacagattcc
      721 tgttttatat gtggcttttt ccagtggatt aagcggctct tacagtagtg cgcttcaagc
      781 ggttgaattg ctgaaggaag aatatgaaga ccctgagatc tatgtttttg atacaaaagc
      841 tgcaagtatt ggggaagggc ttttagtatt ggaagctgta agattgaaaa aagaaggtaa
      901 aagcatcgaa gaaaccctag agtggctgga aagtaattat ttgaaagtcc attcatgggt
      961 aacagtagac gatctgaaat atctagaacg aggcggtagg atttccaaag caagtgctac
     1021 tcttggcagt ctattgagtg taaagcctat tattacggtg gataaagaag gaaggctgac
     1081 gaatgttgac aaagttcgag gaagaaacaa gtcgatccag acaatcgtca atgagacggt
     1141 aaaaacaatc gttgcaccac ttgatcaaat cgtttatgtg gcttacgctg gggatctaga
     1201 gagcgcggag aaggcaaaac ggcttttgga agagcagata aagatgaaag atgtgaagct
     1261 ttatccctta ggaccaacga tcgccagtca cacaggctat ggctgtatcg cgattttttc
     1321 gatgggaatc agtcgataaa agcagtaact cattctgtaa aaaagcccag aaattgattg
     1381 taacaaaaac agcgactctt aaaagataga tgagcatcct atctcttaga ggtcgctgtt
     1441 tttttatttt ttaagctaaa taggccatta tgcccataac tcgttcaatt gtttttgaat
     1501 atctgcattt tccaaaaatt catcatatgt cgtatcagct cgatcgatca cacctttatc
     1561 cgaaacggca atgatgcggt ttgccaatgt ttggataaat tgatggtcat gtgacgcaaa
     1621 taagattgag ccagtaaatg ccatcaagcc gtcattcaat gctgtgattg attctaagtc
     1681 taaatggttc gttggatcat ctaatacaag tacgttagct ttcgaaagca tcatttttga
     1741 aagcatgcaa cggactttct ctccaccaga taaaacattc actggtttca atacttcttc
     1801 accagaaaac aacattcttc ctaagaagct tcttaagaag gtgttatcat cttcttcttt
     1861 acttgcatat tgacgcaacc agtctaaaat cgttagatca gtatcgaatt ctttcgttgt
     1921 atcctttggt aaataagaat tacttgttgt cacaccccaa cgtactgttc cagtatctgg
     1981 cgtaatttca cccatgataa ctttgaatag cgttgttgtt gttatatcat tttctgcgac
     2041 gaaagcgacc ttgtcttctt tgctcaaatt aaaagatata tcatctaaaa ttttctttcc
     2101 atcaatcgtt acacttacat tttctacctg caataagtcg tttccaatct cacgttcagg
     2161 tttgaatcca acgaatggat aacgtctaga agaaggttgg atatcgtcta aagtaatttt
     2221 ttcaagcatt tttttacgtg aagttgcttg ttttgatttt gatgcattcg cactaaagcg
     2281 ggcaataaat tcttggagtt ctttgatttg ttcttctttt ttggcatttg cgtttgcttg
     2341 aagtttagcc gctagttgac tagattccaa ccaaaaatca taatttccta cataaagttt
     2401 gattttacca aaatctagat ccgccatatg tgtacatact ttgttcaaaa agtgacggtc
     2461 atgggataca acgatcacag tattttcaaa attgatcaaa aattcttcca gccaattgat
     2521 cgattggata tccagaccgt ttgttggttc gtctagcaaa agaacatcag gtttaccaaa
     2581 tagtgtttgt gctaataaca cttttacctt ttgtccacca gttaattcac tcatttgctg
     2641 atggtgcaat tcttcaggga tgttcagacc ttgcagtaaa acagctgctt ctggttcagc
     2701 ttcccatcca ttaagctcag caaattctcc ttctaattca gctgctcgaa taccatcttc
     2761 gtcagtgaaa tcctctttca tgtaaatcgc atctttttct ttcagcactt catacagacg
     2821 cttgtgtccc atgatgactg tttcaagtac agtatagtct tcatagtcgt aatggttctg
     2881 tttcaatacg gccatccgtt catctggtcc catagtaacg gaacctgtgg aaggctgcag
     2941 atcccctgat aacactttaa ggaacgttga tttacctgca ccatttgcac caatcaagcc
     3001 atagcagttt ccgggggtaa atttaatatt cacatcatca aatagtttgc ggtctgaaaa
     3061 gtgcaaactc acatcattta cagtaatcaa tttttttcct cacttttatc attattagtt
     3121 ttgacaagtc ttatagaatt atatagggga aaggtaaaag attcaaggca atcgaacatt
     3181 ctttgtgttt cacgtgaaac agaatgaaaa aaataaaaat aaaagcggag cgaaaatagc
     3241 tactatagga tttattagtt tgcaacagca taatttttga cgttatttat aagagggagt
     3301 actataaaac tgtaaaagaa atgaaagaag gattgaaaat ggatgaaaaa agaaccatcg
     3361 actggggctc attgattttg ggaattttat ttgtacttgt ctcattgtta tcatttcaag
     3421 accctgtcgg aaatttagtt gcaattgtag tagtctttgc gatttttgcc tttattaaag
     3481 ggatatttga actgtttgta cggaacagat taaaagagct aacaggatat aaaggaaaga
     3541 tgcccttaat aatcgggatc atcgatattc tagttggcgt ctttttctta ttcaatattg
     3601 gtgcaggagt cgcagcattg ccttttgttt ttgcagtatg gtttattgct gattcagttt
     3661 tagcgttatt tactgctgac ttggcaagag gagtaagtga aggatattat tggtttacga
     3721 tcatcgtcaa tattttagga attttattag gaattttctt attgtttaat ccaatttctt
     3781 ctgcgttgac attaagcttt ttagttggat tctattttat gttatttggt atcacacata
     3841 tcgtctatgc atttagataa gtaaaatcaa acaaataaaa aagccggctt ccgattacag
     3901 ttttactgtt gtaattggaa gccggctttt ttatcatgga caaatatgca cgaggtgatt
     3961 tttaggtgtc gagacaaatt tatgcaaaaa tgtgagaagc tatatacaca aattttatct
     4021 taatactcga agcatagcgc agttgtcaca acctcttttc atataattca ttcaatactg
     4081 gcaaatcatg gacttcatat ttttctttga atgcttggat ttcttcttca gaatattttc
     4141 ttggatctaa atgcagatct tctactggca atgggcgctt gttattgatc ttcacatcga
     4201 ttactacagg acgatctgat tttgcagctg catcaaaggc aaatcgaagt tgcgagtatt
     4261 ctgtgactgt aaagctattt gccccgagcg cttctccaac tttaccgaaa tctgcatctt
     4321 ctaaaaatac accaaatttc ttttgttccg tatcttcttg ttctgcttcg atgaaaccaa
     4381 aagaatcatt cgaaaagaca acattaataa ttggaagctg atatttgact tgtgtaatga
     4441 tatcttgcat gaccatagca aacccgccat caccacttaa ggtaaatact tgtttttctg
     4501 ggtagcttag ttgtgcggca attcctcctg gcacaccatt acccatagtt gcgaaccatc
     4561 cagatgtcgt atgtttttgt ttaccgttca tattcaataa acgaatagaa tggatagtcg
     4621 tgttaccgac atctgtgacg aaaatcgcat cgtcttcagc aatacgattg atttctttat
     4681 agacagcctc tggacgaatc ggttcttcct ctcgatcttc aaaagatttc aaccaattta
     4741 cccatttttc tttattttct tgatttgcta ggtaccagct atcagcaggg cgagcatccc
     4801 ctaactcgac aagtctttgc aaagctgtgt ttgcatcacc gaggatcgac aaagatgttt
     4861 catggcgtcg tccaaatttt gacgcatcga tatcgatttg gataaattca gcagttggat
     4921 taaaaaatgc acgtccaaat gggaagtcgc taccgacaaa taagatcaga tcggcttctg
     4981 ccaatgcttc atttccgggt ttagtagcga cacgtccagc aaaaccaaga aagttttcat
     5041 aatcatcagg aataattcct tttgctagaa cggaagcgac aacaggaact gaaaaatgtt
     5101 ctgagaattc tttgatttgc ggaaatccgt ttcgcgttcc ttgaccaaca taaagaactg
     5161 gtttttttgc tttttcaaga taaggcaaag cagctagcaa atctttttct tctggtaata
     5221 tcacacttgt tttgtgagtg tgagcggtgg agaaaggttg ttcgtcaatt tcttcaaaac
     5281 caagatcaac agggatagtc acgatagcca cgcctttatg ctcataggcg gctttaatgg
     5341 cttcatctac tacatgcgga agactttgtg gtgtcatgac tgtacgattg tatacgctaa
     5401 catctgcgaa aataggattt tcatttagtt cctggaaaga gttatagttc atggatgtag
     5461 aagcaacttg tcctaagaac gctactagag gcacatggtc catctgtgca tcatacaaac
     5521 cattgatcaa atgtgtcgca ccaggtcctg cagagccgaa gacagcgccg actttccccg
     5581 ttaattttgc atctgcagcg gctgcgagtg cgccgacttc ttcatgacga acttgaatgt
     5641 agttgatttc ggttttttct tgatatagcg catccataat tgaattgaaa gatcctccgg
     5701 gaatgccgta aatatggtcg ataccccagc tttcaagaac ttttaccatt gcgattcctg
     5761 cattgatttt tgacatggtg tttcactcct ttagcaatga tgatacataa attttttgta
     5821 tctagagtat agaacttttt atcgaaaatc ccaactaatt cgtcttttca caaaaatgta
     5881 gtcatgtttt tgggaatatg ctagagtagt acatagatag attcaagaga ggggaatggc
     5941 atggttgaat ggctattgag acttgaacct tggcagcaag cacttgctgg gacagctttt
     6001 acttatttta tgacagctct tggtgccggg ttggtttttt tctttaaaga aatcaaaaaa
     6061 gaagtgctaa acttgatgtt aggatttgct tctggtgtca tgattgctgc aagtttttgg
     6121 tctttgttag atccggctat tacaaaagca gaagaaaatg gagatattgc ttggctagtt
     6181 gtaagtattg ggtttggttt aggcggtgtg tttttatata tggcagataa aacactgccc
     6241 catatgcatt ttggaccaca acatgaagca gaaggattgc ctacccattt aaaacggacg
     6301 atcttgcttg ttttttctat tacattgcat aacattcctg aaggtttagc cgtgggggta
     6361 gcattcggtg cagcagcaac tgcggataat ccgacagccg ctatattagc cgctgtttcg
     6421 gtggcgctag gtattggtat ccaaaacttc ccagaaggag cagctgtatc aatcccgttg
     6481 cgacaagaag gtctgagtcg taaaaaggcc tttgtttacg gacaagcttc tggaatcgtt
     6541 gaaccaattg ctggagtgat tggagcgcta ctagtaacga aagtggagct gctgcttcct
     6601 tatgcacttg cttttgccgc aggagcaatg atttatgtcg tggtggaaga attgatccct
     6661 gaagcacagc agacattgtc cagcaaacgt cattttgcag tttttggcgt catgagtgga
     6721 tttatcatta tgatgatttt ggatgtggca ttaggataaa taaaaaaaat atatatgata
     6781 tatcaaatag ggccgcgaac ttatatcgcg accctatttt tttagtaagt tatatcataa
     6841 tgaagtgaat ggtatcatac attaataata gttttttcta tgtgtatttc ctttgatcca
     6901 gcgaacaagc cagatgataa gtgcgattgg gattagaact ttgaccgcaa accataaaag
     6961 cccgcttagg acactgacaa aaagactgat gacgaagcta gctgcaatta gcactaaaag
     7021 aaccatgaag atatttaaga acatattttt ttgacgcata caaaaccctc ctcttgttca
     7081 ttctttacta tagaaaaagt ataacaggga ttgtttaaaa acacctccaa ctgaagactg
     7141 atttttactt catctactta aatgatcaaa caattgcttt ttctctggtt tatcatctaa
     7201 aatagagcgg aaaaggagga gaatatcatg cctaaaaatc gtcttgaagc atttacagat
     7261 gctgtaatcg cgattatcat gactatttta gttttagaac ttcatgaacc taacggagac
     7321 acgtgggagg cgctcgctgc attaggttac cgatttttta tctatattgt cagttttatc
     7381 attttagcga tttattggaa caatcatcat catttatttc aagttgtaca taagataaat
     7441 ggacgggtct tatgggcgaa cagtttcttt gtttttgcgc tttcgctttt tccgtttgca
     7501 acttcatgga tcagtgagta catcaatagc ctggctccag aaatgactta tggttttgtt
     7561 accttgaccg ctaatattgc gtattatatc ttggcaaaag aacttgtcaa agcaaataaa
     7621 ggccacgaga aagcattgaa ggcgatgttg aataattaca agaaatcgta tctatcgatt
     7681 ggtttgaatg tgttaggaat tgtcttaggg tatttagtag cgccagtagc cgtgctgatc
     7741 gcaaacatcg tgattctagc tgcttggatc atcccaagtc gtaccatcga atcacaatat
     7801 cggtaaaatg atccgtgttt aaaatttaag agaaaaatat agaaaaaata aataaacgat
     7861 caaatatccg agtgatacta gtattcaaag agggatgcag atggatcaaa aaagaattca
     7921 tgtaggactg attactagtt ttgtcacgat gcttatttat ttggatcaag gtcatttgac
     7981 ttggacagta gttggtgtcg taggattttt gacattattc acagcaataa aaaaataaac
     8041 atctttatgt aatctgatgt tctatctatt taagaaaagc atgtttacag cagtatgatt
     8101 ctgttgtaaa cgtgttttta tttgtggaaa atttaatttt tagaaaagtg tatcgaaaaa
     8161 tagatagtta gtttttccac agtggataac ttcgtaagag ataaaaataa aaaattagtc
     8221 aaagaagggc gtatagattt cttttgat
//
LOCUS       DARBEC010000108         8172 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.112, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000108 DARBEC010000000
VERSION     DARBEC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8172
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.112"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            94..1248
                     /locus_tag="SCL80_002488"
     CDS             94..1248
                     /locus_tag="SCL80_002488"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEG1108326.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYGLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            complement(1270..2022)
                     /locus_tag="SCL80_002489"
     CDS             complement(1270..2022)
                     /locus_tag="SCL80_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEG1108327.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(2386..5052)
                     /locus_tag="SCL80_002490"
     CDS             complement(2386..5052)
                     /locus_tag="SCL80_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEG1108328.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            5393..6844
                     /gene="cls"
                     /locus_tag="SCL80_002491"
     CDS             5393..6844
                     /gene="cls"
                     /locus_tag="SCL80_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEG1108329.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCL80_002492"
     CDS             complement(7173..7988)
                     /gene="thiD"
                     /locus_tag="SCL80_002492"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEG1108330.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
BASE COUNT         2373 a         1697 c         1457 g         2645 t
ORIGIN      
        1 aatccttaaa tattctcttt tttatatttc ttttaatctt tttatgatac cttagtaaag
       61 taagtttttt atcataagta aagaggtgtt gaaatgaaaa tagggattga tcgtctttcc
      121 ttttttattc ctaatttata tttagatatg acagagttgg cagaaagccg tggggatgat
      181 cctgcaaaat accatatagg gattggtcaa gaccaaatgg cagtcaatcg tgcaaatgaa
      241 gacatcatta cactaggagc aaatgctgcc agcaaaatcg taacagaaaa agaccgtgag
      301 ctaatcgaca tggtcatcgt cggaacagaa tccgggattg atcattcaaa agcgagtgcg
      361 gtaattatcc accatttact gaaaatccaa tcttttgctc gttcttttga agtaaaagag
      421 gcttgctacg gtggcaccgc agctttgcac atggcgaaag aatatgtcaa aaatcatcca
      481 gaacgaaaag tactagtcat agcaagtgat attgctcgtt acggcttggc aagcggtggt
      541 gaagtgacgc aaggtgtcgg tgctgttgcg atgatgatca ctcaaaaccc gcgtatttta
      601 tcgattgaag acgacagcgt atttctgaca gaagatatct atgatttctg gcgtccagat
      661 tatagcgaat ttcctgttgt tgatggtcct ttatctaatt ctacgtatat cgaatcattc
      721 caaaaagttt ggaatcgaca taaagaattg tcgggtcgag gactcgaaga ttatcaggcg
      781 attgctttcc atattccgta tactaagatg ggaaaaaagg cattgcaaag cgtattagac
      841 caaacagacg aagaaaatca ggaacgtctt atggctcgct atgaagaaag catccgttac
      901 agccgacgaa tcggtaatct ttacactggt tcattatacc tggggctaac ttctctactg
      961 gaaaattcga aatcactaca gccaggagat cgcatcggtc tattcagcta cggttctggt
     1021 gcagtaagtg aattttttac aggttatctg gaagaaaact atcaagaata tctcttcgca
     1081 caatctcatc aagagatgct agattctcga acacggatca ctgttgacga atatgaaacg
     1141 attttcagtg agacgcttcc tgaacacgga gaatgtgcag aatatacttc agatgtacca
     1201 ttttcgatta caaagatcga aaatgatatt cgttactaca aaatataata aaatcataaa
     1261 aagaaccctt cacgagtcgt gaaaggttct ttttttatct gcggaccaaa ggagcagtct
     1321 ataggtcagc ttagtcgtac taaggatttc tcgtttttca ctgaatagtt tgcgaatgta
     1381 cctggtcaca acaggatctt ttgcgtacgc gtcatagtat agcttcatca attcttcagt
     1441 aattgattcc tgtgtaaaat aagtaaaatc ttttttcttc actttgaatc cttcatcaat
     1501 taagtatgtt tcaaattgat ccattacaac tggcgatgta ttaggatctt cttctatcat
     1561 tcctagttgc ttttcgatag gataaaaaac atcatcgatt gattccacta ctctgattat
     1621 cacgcaccat ttgcttgact ttttcatcaa tttttctaat tgaataagat gtaccaatgc
     1681 tggatttgct gctgtaaaaa ccatttcata tgtctcattt gattggataa attcttctaa
     1741 cgactgtttg ataaaggaaa catttggcaa ctcatttttt tctgcttctt tttttgcatt
     1801 ttctagcatt cctgaagaaa aatcgatcaa atcatagcga ttaatttctt ttgctaagga
     1861 aagaaatctg ccagatcctc cagcaacgtc tgctgctgtt tcagcaggca ggactttctc
     1921 cgttttcaga taatttttta catcttcagc aatagttact tgcgattctt gctggatttc
     1981 ataatattct tcagcaaatt catcccattc tttttcattc atttgatttc ctctaatttt
     2041 ttgttatatg ttttcacttt tcggaataaa tcattctcct cttttatcta ttctatctct
     2101 tttgatgaat aataaaatga ttggctttcc gtctatcata aataaaaaaa gggactgtga
     2161 ccaaagtcgt gtcaaagtcc ctctttccga ataaacagtg tccaatcaga tgaccctcag
     2221 tattagctca aaatgctgat tttacaactt cttccttaaa cggtgttctc ctgccaactc
     2281 ttcggtcaca agtcacaata ttcaacaaac atgagaagct gtttgttgaa attgcacctt
     2341 gtgactcaga attaaacggc aggctcacaa cctcgttgat tcgttttatt tatttccttg
     2401 tactcgtaca atgatcaatt tagtgatttc catcaaaaca attgtcacaa gtgcgattcc
     2461 catacatgta aagacttctg tcaatccaaa tgctgatggg atagagaaga cttctttcag
     2521 tcctgggatc attgttaagc agtaaagcgc gctacataca actacagcta gtaagacact
     2581 tctgttagag aagaaccctg cttggatagc tgtttgtgtg tttgaacgtg ctgggaatgt
     2641 ttgcaatgtc cgacaccaaa tcaatgttga aaatgacatg gctacaccga attcaggtga
     2701 atgctgcatg ccaagccatt gagcaaagat aacagcaatc ccaatcagca caccacgata
     2761 cgttacagaa atcaatgttt tgtccgtaaa gattcctgaa tttggatctc ttggcgggcg
     2821 gttcatcgta gcaggttcag ctttttctgt acctaatgca attgctggca gtgaatcatt
     2881 gactaaattg atgaacaaca gttgtaatgc tgtaaatgga ttggaccagt cagcaactaa
     2941 ggcaaagatg atcgcaatga tcgcacctaa atttcctgca aatagataag aaatagcttt
     3001 cttgatatta tcaaacacat tacgtcctac ttctactgcg ctgacaatag aagcaaagtt
     3061 atcatccgtc aataccatcg ctgctgcatc ttttgccaca tctgtacccg ttcccatagc
     3121 aattccgatg tctgcttgtt tcaatgctgg tgcgtcattt acaccatcac ctgtcatagc
     3181 tgagatatga cctttatttt gccaagcacg aacgatacga attttattct caggtgaaac
     3241 acgtgcataa acagtgactt tttctaaaac ttcgtttagt tgatcttctg tcaattcatc
     3301 tagttccgtc ccggtcaaag ccaggtttcc ttcttcaaaa atcccgattt gttttgcgat
     3361 agcaacagct gttgttttgt ggtcaccagt aatcatgatt gttttgattc ctgcacttct
     3421 tgcttctgtt accgcctgag tcacttcagc acgtggagga tcgatcatag caagcaaacc
     3481 aacaaggatc aaatcgtctt catcttcaaa tgataaatca aaattatcaa gtggacggta
     3541 agcaaaagct agtacacgta atgcgcgtgt tgaaaaagct tcattttgct cttggaattt
     3601 tttcatcaat tcatccgtca tcggaacgac ttcaccattt agcaatactt ttttactgcg
     3661 ttggaagacg atatctggtc cgcctttaac aaacaagtaa tttttgttat caattgtgtg
     3721 tccagtagac attaattttc gatctgagtc aaaaggaagt tctgcttgtc ttgggtagtt
     3781 gtttcggatt tcctgataag gctggtttaa tttttcacta aagtcgataa acgctacttc
     3841 cgtagggtcg cccaatttag taccgtcttc actgatcgat gcatcattcg ctaaaacact
     3901 gatttcgatc aagcgacgtt cgtcagctgt ccattttgaa gggtcatttg tgaaatcccc
     3961 agtatcgcca ttacctaaaa agtagtcaac aactgtcatt ttgttttgtg tcaatgtacc
     4021 agtcttatct gtacagatga tacttgtcga accaagtgtt tcaactgctg gaagcttccg
     4081 aataatggca tgacgttttg ccattttatt cgttcccaaa gataacacga ttgttacgat
     4141 agactgtaat gcttctggaa tagcagctac ggctacggct acggcaaaca tcactgcgct
     4201 gacgatatct gcagtcatat ctcctgtacc ttctccaaga taaatacgaa acagttggat
     4261 acctaaaatc aataaagaca atgccaaaat agccaaactt agttttttac tgaatttatc
     4321 cagacctcgt tgtaacggag tgacttgctc agttgttgat tccaaaaggc cagcaacttg
     4381 tccgatctcc gtattattcc cagttgaagt cactaagaat ttaccgcgtc cgtaagttac
     4441 gatcgttcca ctgaatacca tatttgtacg atctccgatt ggtaccgtac ttgtgatatc
     4501 cgttacttct ttttctgcag aagtcgactc tcctgtcagc ataccttcat ctacttttaa
     4561 tgatcccgca tctagtaaac gtccatcagc aggtacataa tcgcctgcgt ctaacaacac
     4621 gatatctcct accacaagtt cgtttgccgg aatcgttttt tccttaccat cacgaatcac
     4681 tcgagcatcg ggcgctgaaa ggtttttcaa cgcttctaaa gaaccttctg ctttttttgt
     4741 ttggacaaca ctgattatag aattcaacat caatactgca aaaataacta agaattcgat
     4801 gtagtcaccc atgaccatct gaatcagtgc gacaatcagc aatacgataa ccattgcatc
     4861 tttgaaagtt tctaagaaca atttccaagt tggcttcttc ggcttttctt ttagctggtt
     4921 caagccatct ctttctaatt tttccttcgc ttgcgtggtg ctcaatccat cggttgagct
     4981 ctgcgtttgc tgcatcaacg agtcgatcgt ttctttatag accgcttgtt gtgcttgttc
     5041 cttgttttcc aaatttcatc ccccattttt tgatcttggc cctagtcctg tctgaaaaaa
     5101 aagagaccta agacaaatac gcatttgtct taggtctcac catttcatac aataccagaa
     5161 aaccatttgg ttaacttact gttgatatta tcgcaacaaa cgttgccggt tactccccta
     5221 aggagatatt caactgtact aatatcctac cttattaacc tacaaaaagc aactacttta
     5281 gcccgattta cctacatttt catgaaagga gtgaaaagta tattcacctt ggtaggcttc
     5341 tttcttctat catgctatac taatgttatt gtatgtaagg aggaacattt ttgtggtatc
     5401 tagtattata accgcccttt atctattaaa tgcacttatt gctttggtgg ctattttgat
     5461 caaaccccga gatgtagcag ccatttgggc atggctctta gtattgtttg cccttcctgg
     5521 ggtgggcttt gtattatatt tatttttcgg acgtggatta acggataaga aaaaattcta
     5581 tctccgacaa agtgacttga aagaattaga aaactttcag tcttttagag gagataccat
     5641 tgaacattac gatcctgaca tgggcgataa agacaaacag caatttgttg acttcttctc
     5701 ttcattaaat cgtatgccgc tgacaagaat gaattctgtc actcttctca cagacggaca
     5761 agagaaattg gattcactgc ttcaagatct aaaaaaagcc aaacattcga tccatatcga
     5821 atattacgca tttgtgacag ataatatcgg ccagcaagtc ttacatgttt tagaagaaaa
     5881 agccgcagaa ggcgtggaag ttcgaatatt atatgatgca tttggctctc atggcacaaa
     5941 agcaaaagat ttcaatcgtc taatcaaaaa tggtggacat gtccatacat ttgttacctc
     6001 acaaagggca ttacttcgtt tccgattgaa ttaccatgat caccgaaaaa tcgttgtgat
     6061 cgatggaaag attagttata ccggtggttt caatattgcc aatcaatatg taaatacaac
     6121 aaaaaaattc ggctattggc gcgatacgca tatacggatt ttcggtgccg cttcttcttt
     6181 gctccagctt cgcttcttaa cagactggaa cgtctcggta cctgaagaaa aaaaggtcgg
     6241 ctatcatttg aattatttct ttaaaaaagc agatcgagat gaatctaagc ttgctgatac
     6301 atccatccag cttgtttcaa gcggaccgaa taacgaaagg gaacaaatca agctttcatt
     6361 tatcaaattg attacttctg ctaaaaaacg tgtttggata cagacacctt accttgttcc
     6421 tgatgaaagt gtcattgctg ctttaaaaat cgcaactgcc tctggtgtag atgtgaaaat
     6481 catgattccc aacaaaccgg atcatccttt tatttatcga gcaacacaat attatgctcg
     6541 gcagctgatc aaggaaaatg tacaaatcct tgtctatgag aacggcttcc tccatgcaaa
     6601 aacattgata atggatgatg aaatctgcat ggtaggttca gcaaatcaag atattcgtag
     6661 ctaccgattg aattttgaaa caagtgctgt catttacgat cctgagtttt tagaagaact
     6721 tgctactcag ttcaaagaag atgagacaca ttgttcatcc atgacaactg aaacagtcaa
     6781 ggaaatgtct aactggctat tattcaagca acaaatttct cgattatttt ctccaatcct
     6841 ataatttttt tattcaagta gagaaaaata tactaagatt aaagggactg tgacaaaaat
     6901 cttgtcacag tcccttcttc cgaacaaacg gtgttctcct gccaactcct cggtcacaag
     6961 tcacaatatt caacaaacat gagaagctgt tttttgaact tgttccttgt gactcggagc
     7021 tggacggcag gctcacgacc tctttcttaa acggtgttca aaagcttgca tctgcggtaa
     7081 taagtccaac caagttaaaa acataagaag ctgtttttac ttggctgttc ttattacttg
     7141 atgcagggca actttttcac aacttctttc ttttactcta attcaccagc tgcccaatga
     7201 ttgatggggc catatttgtg ccctacattt atttcttcac gaattgcacg gtttacatag
     7261 tcttttgcca atcggatcgc ttgttcaact gtttttcctt tcgctaactc agctgcaata
     7321 catgcagata gcgaatcgcc agttccgttg atccggtcgg taggataaaa tggtgcattt
     7381 aaccaaaatg attttcctga ttcgaacaat acgtagtcat tgactgtttc ttgctgcgca
     7441 tttttagcat gatgtccttt gatcataacg ttttttgcac ccatatcttg cagttctttt
     7501 gctgcttgca caaaatcttc ttcagctgct aatgtccgtc cagtcagctt ttctgcttca
     7561 taaaaattag gtgtcagtac ttcggccaaa gggacaagct tttctttcaa gctttgcaaa
     7621 gcagattctt ctagtaaaag atttccatgt ttcgttacga ttacgggatc cacgactaat
     7681 ggtccaaaat catatttttg gtaatttttt acaactgtct caatcaactt tgaatctgct
     7741 agcatacctg ttttagctgc acgtatttga aagtccttcg caatcagttc aaattctttg
     7801 tcgataaaat ctgttggcaa tgtcacactt gctccgattc cgtaagaatt acctgcaaca
     7861 catgcggtca tgacagaaac accgtaaaca tttcttgtaa agaatgtatg catatctgct
     7921 tgcatccctg cgcttccatc gctatctgat cctgcgatcg tcattgcttg tacatactga
     7981 tcttccatca aatcgattcc tccattttta taaaaagatt tttcataata ctgttactgt
     8041 ttctatcata tgtttttcaa agtatccctc agtaaatata ccactacctt ttcttggtaa
     8101 tcaaatttat ttgaaaaaac atttgcaata aatcagaaaa tagtggtcgt ttatttgtat
     8161 tcgctattct aa
//
LOCUS       DARBEC010000109         8077 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.113, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000109 DARBEC010000000
VERSION     DARBEC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.113"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            100..633
                     /locus_tag="SCL80_002493"
     CDS             100..633
                     /locus_tag="SCL80_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108331.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(759..1436)
                     /gene="nth"
                     /locus_tag="SCL80_002494"
     CDS             complement(759..1436)
                     /gene="nth"
                     /locus_tag="SCL80_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEG1108332.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(1443..2141)
                     /locus_tag="SCL80_002495"
     CDS             complement(1443..2141)
                     /locus_tag="SCL80_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEG1108333.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2409..3593
                     /locus_tag="SCL80_002496"
     CDS             2409..3593
                     /locus_tag="SCL80_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEG1108334.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            complement(3599..4042)
                     /locus_tag="SCL80_002497"
     CDS             complement(3599..4042)
                     /locus_tag="SCL80_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1108335.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(4268..4555)
                     /locus_tag="SCL80_002498"
     CDS             complement(4268..4555)
                     /locus_tag="SCL80_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108336.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
     gene            complement(4726..6078)
                     /locus_tag="SCL80_002499"
     CDS             complement(4726..6078)
                     /locus_tag="SCL80_002499"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEG1108337.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            complement(6351..6797)
                     /locus_tag="SCL80_002500"
     CDS             complement(6351..6797)
                     /locus_tag="SCL80_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1108338.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            complement(7033..7959)
                     /gene="cysK"
                     /locus_tag="SCL80_002501"
     CDS             complement(7033..7959)
                     /gene="cysK"
                     /locus_tag="SCL80_002501"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEG1108339.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
BASE COUNT         2205 a         1624 c         1299 g         2949 t
ORIGIN      
        1 tacttgttta taattaaatc ggctaaatac acactagtct tttatttagt gccacttctt
       61 aataatccca ttgttattat caaataaaaa atacaatata tggttttgaa taattttttg
      121 aaagaaggct tcattttgag ttatctgata gtatgcttaa ttatcataat ttgtctgcac
      181 catctttttt tatcgaaaac aatacctcgt tattccaaga ataaaaaagc tgaaaaattt
      241 tgtttattat taaataaatt tactttaatt gctcctatcc ttgcatttat tattttttct
      301 gtacttttaa gcactactct taaaggaaaa tttatggaaa gaagctctca tgcaatgatt
      361 ctcacttttt tatggttgtt gttcaccaga atctatattt ttctgatgag cctgaaaccg
      421 cccaaatcca ttagtttatg tttagttata aatggtattt ttttattatc acttatcatt
      481 tttattacac ctttagatag atacgtcact tatttatata atcctttgga atactggact
      541 tattttatag gtatattgga aggcattatt ttctacatcg ggtattttcc caataaaaat
      601 aacaactttt atttttatcg caataaattg taataagtta ccatcctagc tactgagtat
      661 gtacatttac tttttcatgc aaaaaagata aacgaaacag cttttctcca taataacaag
      721 ataattcata catttatttg caattaaaga aaccacgtct acaggcgtgg tttctttttt
      781 agcgcctttt cttttagacg catccgattt ttcccatctt gacatattga tagcaaagga
      841 catacctcgc acttgggatt tcttgccgtg caatgatatc taccaaaaaa aatcagtgta
      901 tgatgtgtct ttacccataa ttcttgtggt actttccgca tcagtgtttc ttctacttcc
      961 atgaccgtag catctagttt gcagatcctc aggcgttttg agacacgttc cacgtgggta
     1021 tctactgcga ttgctggtat cccgaatgca tcccccaata cgacatttgc tgtctttctg
     1081 cctacaccag gaagagacat caattcttct cttgacgttg gcacttgccc atcgaaacgt
     1141 tcaatcagct gctgtgcaca cgctttgatg ttttttgctt tatttcgata caaaccaatc
     1201 gtttttattt taagaatgat ttcatcgatt gaagcttctg ctaacgcatc gggtgtcgga
     1261 aaagaggcaa acaaatctgg cgttgcttta ttcactgata catctgtcgc ctgggcactt
     1321 aagatcactg caatcaataa ctcaaatgga ttattgtgct tgagttctcc atgtgcttcg
     1381 ggaaacattc catacatcgt ttctagagct tccattgttt tttgtttgct taacatattc
     1441 cgctactcct ccaaccaatt tttcagtgag actttcggca attcttcttg ttgagctgac
     1501 tgttctactt ctctttgaag aagctgctgt tttcttcgtt tttggtcttc ttccacttgt
     1561 tgttttgtac gtagattttt tctttcccac gatagcaaaa tgcggtcaac gtaattaaaa
     1621 ctataagcct gatttaatac ggcttcccgc agtgctagct tcaaaatttc cggctgataa
     1681 tggtcttctt ctagccattg tccgatccgc tggaactcga tcgaagaaag cgggcgtcca
     1741 aattcttgtt caaataattg atacactgac tggatttctt tttcttgtgt tttttcttcg
     1801 tatttttttt cttgtgtttt taaatactca cctaatagat catagatggg gtaaagatta
     1861 tatcgatctg cttttttacc gttgacaatc gtcgtttcta tcttgataaa tccatttgtt
     1921 acgagacgat ccagtatttg aaaaatctga tcttgtttga ttcccatatc catagcaatc
     1981 atctgcaaat cagggaaagt atctccttca agctgtgcca tatgcaactg tagaacaaat
     2041 aaaaattctt cagattgtag accgatccta tgataatttt taagtaaaag attagatatg
     2101 actgtttgtc cagcgcttaa gtaattttct aaattcaaca tgatttctcc tccgctcaat
     2161 cacagtatac gtaaaaaaaa catgggaagc aagaacaggt ttcttttcta tttacttcac
     2221 tgatcggtca gatttacaat gtcgtctact tgatttttgt ttgttcatgt ttttgatgcg
     2281 tatcttcact tttcggaata aataaattaa gtagatttcg ctgttcctta gaatgttgac
     2341 aaggaaacaa aattactata cactattttt gtttccaagt taacattatt gcataaagga
     2401 gtttttacat gtacagtttc acaaataaac attctaaagt ttttggatac gtctctgtct
     2461 tattcactgg attaggattt actatcgtca gtcctgtact tccgttttta acgctgcctt
     2521 attctcactc catacatcag caagctttct atatcacttt gctcatgtct gtttatgctc
     2581 tagctgcctt cttatctgcc cccattctcg gtagcttaag tgatcatttt gggcgtcatc
     2641 cgatccttat tatcagttta ttaggatcaa gtatcggata tttgcttttt ggtttaggaa
     2701 attcgatatg gatgttgttc cttggtcgta tcattgaagg attgacggca ggagaaatct
     2761 ccaccttata tgcttacttt gccgacatca cggaacctaa tgaaagaacc aaagtttttg
     2821 gatggatggg tgctttagtc ggtataggaa caacgctggg tcctattata gggggtctat
     2881 tagctgagtt ggggaattct gttcctatat ttattggagc actctttact ttcttaaatg
     2941 ccgtttatgg ctacacattt atgctagaaa gccttccaat aaaaaaacgt tcagtagatt
     3001 taagtttctc ccatgtaaga ccttttcacc aattacaaca attattcaag atttcatccg
     3061 ttattccttt attgacagca ggttttgctg tttggttggc agctggctct cttcaatcta
     3121 tcttttctca attttcgatt gacgcctttc agtggaaggc tgggttggtt ggtttaagct
     3181 tctctttgat tggaatcttg gatactgtct cgcaattgtt cattatgcct cgattgctca
     3241 aaaaattttc agaacagcag atcacaagaa tagggatgtt cagtgaaatc ctcgcctatc
     3301 tattcatcac attatctgga attcttctac tgccgatttt gtttttattt gggattatct
     3361 gttatggttt cggtgattct atttttactc ctgtattcaa tggccaacta tccaattctg
     3421 tctcagataa ccagcaaggt cttgtaatgg gcggtaccca aagtatacaa tctctatcgc
     3481 gcgtcatcgg cccgctaatc gcaggacagc tttatgctgc agcaccattt cttccgacgg
     3541 tattcggatt tttgcttatg atttttgcct attatcacta caataaaaaa tagtagcttc
     3601 atttgctact tcgtataaat tctctgtatc gaccgataaa acgaaatact gtgtcaaatt
     3661 cttcttttga catattttca aatacagtag catctcgctg tacccatttt tgatggagtt
     3721 gctcgtgtct attccttatt ttttgtccga ttggcgtcaa ttgaaaatag atttcttttt
     3781 tattctcttc gctttgataa gtgtttatga tccctctagc tatcagtttt tttgttagct
     3841 tactgatggc tcctctagtc atgtaactag cattcgctaa ttttgtcacg ttaggatcag
     3901 ggattttccc gatatgttca atcacttcta gttcatttag agtaaatcct gcaaattctt
     3961 tttccatttc tggtttatta cgatgaataa tctcattagc taaatccaga tattcttcta
     4021 ttagcagttg tcttttatcc atttctacac cttctttttc tttcattata ttgttttttt
     4081 gtttcctatt caacaaagat aaaaatattt tgtaggaaaa cccaatctga aaccaagaaa
     4141 aaaaggggtt gacaaagctt gtcataaccc ctttttctga ttaaaagata gtaaagagac
     4201 agtcatcggt aattgtgatg attttttgtc atcacttaag gatgagcggc cttttcaaaa
     4261 actgtcttta aagatcatca tcagtttcta gcacatatgg ctgaccttca tatgtgtact
     4321 ctgtattcaa tgtgccgtca ttttcatctg agtagatgcg tctactgaaa ggaaaacgat
     4381 gagaaacatt atctttggcg tcatttgctg gcatcgtgta atattcctct tgcgtttcat
     4441 tcaactgaga gagtacttga tctgccaact catcaatatt gatcgttaat ttcttcgaag
     4501 cacttttata atcttctaaa aatgcatctt gttggacaaa atattcttta ttcatcttct
     4561 ttctcacctt tcttttcaaa atcaaataaa gctgcttgtt cttttatgta acagctttat
     4621 ctttgctgct tattgtgaca aaaaattcga agaaagacaa ctaattgtac ttttcacaaa
     4681 aaaagacaga gggtcgtgac aaaattgtgt cacaaccctt tttctttatt gtgcagtttc
     4741 gatttcgcct agcgctgctt gtgcaagtag gttcaagtaa ttccatgggc ggtcaaaatg
     4801 tggttggaag aagaaatctg aaagtgcgag atcttctaca gtcattttat tttgaacagc
     4861 taaagacatg gtatttgctg attgtgtaat gtcgtatttg gacatgaact gagcacctac
     4921 gatacgattc gttccttttt catagactag ctccatcatt actttttcag tagttggcat
     4981 gaactctgga cgataattat cttcaaatac agtcgcttgg acttctagat cgttcaaagt
     5041 tgcgctttct ttggtcacac ctgtcgaacc gatcgtccag ccaaataggt aaagtcctga
     5101 agttccttga gtaccacgat acgccatttt atgatctgtc aagttatttc ccacaagcaa
     5161 accttgacga acggcatttg ttgccaaagg aatatagttt tgcgtgccac ttggattgta
     5221 gttgactact gcagagtctc cagctgcaaa aatgtctgga ttacttgtct gcatgtattc
     5281 attcactttg attgccccgt ttggcagtgt atctactttt ccttcgatca gttttgtatt
     5341 tggacggaag cccacacata aaatgaccat atctgtatca aatgtatctg ttggtgtagc
     5401 tacttgtttc acttttcctt cttcatctgt gataaattca gtaacatttt cgcctagcgc
     5461 aagagtaaca ccgcggtctg tcaattcttt ttctaagata tctgtgaatg gtttatctag
     5521 atatttattc aagatgcgat ctaatccatc aatcaatgtc acttgttttc cagattcgac
     5581 aaaagcttct actaattcaa taccgatata tccaccacca acaattacta cacgttttgc
     5641 ttctttcgct tgtgcaataa tttcatttgc ttgattgtag tttttgcata ggagtacatt
     5701 tttcgcatcg attccactaa tcggtgggat aataggccat gaaccagttg tcatcactaa
     5761 tttatcataa gaaaccgttt ctgtttctcc tgttttcaga tcttttgccg ttactgtttt
     5821 attttctgta tcgacgtctg ttacatcgtg ttccatttta actgttgctc ctaatgaaga
     5881 taattcttca ggatttgagt agaacaagcc tgctgggtcc tttactactc cgccgacata
     5941 aagtgcgata ccgcaagata agaaagaaat attgtcattt ctttcatata ctgtcacttc
     6001 tgcttctgga tgatttttta aaatactttt tacagctgca gtacctgcat gagtacagcc
     6061 aattactact actttcattt atggtccctc cgattaacat tttgtccttc gacaaaacta
     6121 ctataacaga aataaaacgg atacaacagt ttaatgcttg tgaattcaca aaggtttgtt
     6181 tgtagtttca ttccttttca agcataagaa cccaatatct acaagattct tattttaata
     6241 atattcactt ttatatctaa gattgtgaat aaaagacaaa aaaataaaaa agctagcgtt
     6301 ttttttacac agctagcttt ttttaggtat aacacttgta gaaactgttt ttacatttgc
     6361 gccttttttg ctgactcttt acaatccgga cagagtccat acaattccaa ccgatgttca
     6421 ttaatctcaa aatcagtcag tctgcttgca gccatttcta tatcttcaag ccctgggtaa
     6481 tgaaaatcaa caatcttacc gcagttttca caaattacat gataatgacg ctttgaactg
     6541 aaatcaaatc ggctagaagc atcgccgtac ttcatttctt gaacaaatcc aatcgaagta
     6601 aataagcgaa ggttattgta aacagtcgca acactcatat ttggaaaccg atgttcaagt
     6661 gctttataaa tctcatcagc cgttggatgg gaatgatgtt caatcaaata ttctaaaacc
     6721 gcgtaccgtt gaggtgtgat tcgaatgttc tcttttttta acttttcgat aacctcttcg
     6781 actaacttat tttccatgta aacaccccac gttgttattc tcgtcttatc atactatttt
     6841 tacattttaa aatccactta acatgaaaag aaaattcaat tttattgtat tttaatgaaa
     6901 aaagacgtaa aacagtcgcg ttcactattc tacgtctcta ttcaaataat ttttctcatc
     6961 ataaattttt gtgtgttctt ttcatcttta aaagtttttt acataaacct atctggagaa
     7021 aaaccaagtg ccttatatct cttggtaaag ggctgtagat aaataacgtt caccattgtc
     7081 gggaaccaca gctaatacct tcttgccttt tcctaattct tttgcgactt ttagcgcggc
     7141 agcaattgca gcgccagatg agatgcctac taaaatccct tcttttcgcc caacttctct
     7201 agctgtttcc atcgcttcgt ctccactaat agaaaggact ttatcataca cagaagtatc
     7261 cagtgtttta ggaacaaaac ctgtaccgat tccttggatt ttgtgaggtc ccgcttcttt
     7321 cccttctaaa atagcagatt ctgctggttc taccccgatt aattcgattt tcggatacac
     7381 acgttttaat tctcggccag cgccagtgat cgttcctcct gtaccgatac ctgcaacaaa
     7441 cgcgtccaag ccatttactc caaatgcttg atgtatctca ggaccagtcg tcttctcatg
     7501 cacaagagga tttgcttcat tttcaaattg taatggtaaa aagtagccgt tttcttttgc
     7561 caaacgttct gcttctctga ttgatccaga aataccatcc gcaccaggag tcaaaatcaa
     7621 ctctgcgccg taccctttca tcaataaacg tctttcgata ctcatcgtct caggcatcac
     7681 gatgattact ttatatcctt ttgcaactcc taccatcgac aatccgattc ctgtatttcc
     7741 agaagtaggt tcaatgatag tatcacctgg tttcagtaga ccatcgtgtt ctgctttttc
     7801 tatcatactt aaagcaatac gatcttttac acttcctcct ggattaaaaa actcaagctt
     7861 tacaaaaaca tctgctgaat cttctggtac gattttattt aatttgacga ttggcgtttt
     7921 gccaatcaat tcggtgactg agttatagat agctgtcatc ttcaattcct ccttgtcttt
     7981 gctcttctta cttatcataa tacaatctgt tcatttatgg cgagtgattc gtctttgact
     8041 agcaaaaaaa agacaaagat ataccatata tggtaat
//
LOCUS       DARBEC010000110         7896 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.115, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000110 DARBEC010000000
VERSION     DARBEC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7896)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7896)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7896
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.115"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(22..996)
                     /locus_tag="SCL80_002502"
     CDS             complement(22..996)
                     /locus_tag="SCL80_002502"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEG1108340.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(1076..1951)
                     /locus_tag="SCL80_002503"
     CDS             complement(1076..1951)
                     /locus_tag="SCL80_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEG1108341.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            2081..3988
                     /locus_tag="SCL80_002504"
     CDS             2081..3988
                     /locus_tag="SCL80_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEG1108342.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(4529..7807)
                     /locus_tag="SCL80_002505"
     CDS             complement(4529..7807)
                     /locus_tag="SCL80_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEG1108343.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEVPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLESYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
BASE COUNT         2234 a         1665 c         1463 g         2534 t
ORIGIN      
        1 ttctttccct cttttttttg tttattcgaa ttctttttct tccagacgga ataacggact
       61 catcggtgta ttttcatgga tgcgtttgac cgcttcaccc attaattctg aacatgtaac
      121 gattgttaag ttttctggat gacgattttc ttctgtgaaa acagaatcag tgatacaaat
      181 atctttgatt ggtgcattgt ctaaaatact tttcgcatga tcggacaata atccatgtga
      241 cgcacaggca tatacttctt ttgcgccgtt ttcttttaat acatttgctg ctcgtgcaag
      301 cgttgctcct gtgttcaaga tatcatctac catgatacat gtctttcctt gaacatcacc
      361 gatcacataa ccaccctcag ttgaagcatc atctgcatga tctacgatgg ctaatgtgct
      421 gttcaaatat tctgaaagac tacgtgcacg ctgaacaccg ctgttttttg gcgaaacaac
      481 tacgatgtca tctcccgtca gacctaattg acggtaataa tgagcaaaca acggcatcgt
      541 aaataaatta tctacaggaa tatcaaagaa tccttgtact tgaaccgtgt gcagatctaa
      601 agtcagtact cgagttgccc ctgcttcttc gatcaagtta gcgatcaatt tcgcagtgat
      661 cggttcatga ggtttcgccg tgcggtcttg cctagcatac ccatagtaag gcaatacgac
      721 attgatcgtt ttcgcacttg ctcgcttcat tgcatcgatc atgatcagta gttccatata
      781 gtagtcattg acaggtgcat tcgttgattg aacgatatac acatgatccc ctcgaatact
      841 ttcttcgata ttgatggaaa tctcgccatc actaaattgc ttcacgacgc ttttacctaa
      901 ttctgttccg aacactttcg cgattttttc agctaatgga cggttgccat tcaaactaaa
      961 aatcttcaac gtgtcatcct gatacttatt actcatgtag tcctccccaa aacttctcta
     1021 ttcttcgata gtttaacata tttttccaag attttctgtt aatttcagca aattcttaag
     1081 gaagtgaaac tgttccgtta ctccaactgg caggaatgac tttatgataa tcgccccagt
     1141 tgtcgtgtac tttgtaataa cgttctcctg tttcagtttc aaaataagcg tacgctgtca
     1201 cccaatgatt gccatatgta ctgccaagta ctttcaaaat accaatcatc acaggtttgc
     1261 cttcacgtat acgttttgta gctcgctgcc atgaacctag aagagttgct cgtgcacgct
     1321 catgattgcc attcttcttt aaaaaactag aaatccctgt actaacctga aaaggaatcg
     1381 taggaagtcc tagtggttgg atctggctac gcaatgcctt cgtcaaaacc aacgaatcag
     1441 ccgtattttt cttgcgaatc tcatttggaa gcatccaccc cttacgatag tcttgataat
     1501 aggcaaggag aactgctgcc gcgtaagtac cacaaagata gcctgagggg gtagcccatt
     1561 tcttatacct caaaaaacgt tcttctggca atcccaccca gcaatttgcg agttcctttc
     1621 ctgacctttt ttcttcgatc aaaacgggaa tatcttcttt tctgttgatc catctagata
     1681 actcgattgg ctcttcattg atcagcttcc attttttaca agaaagaccc tcttgataag
     1741 tatattttgc tggagaatat tctaccggat gtcctgattg atcgataatc acataacttt
     1801 gatcttcaaa aaccagagct aaaaaaagca atttactttt tcttgactgc agctgctgag
     1861 tgctggctaa ttttttaatt tttttatcta ctttgaaata taaaggttta tccactactt
     1921 gattcagttg ggatcgcctc ctttttttca ttataagtga aggattgaaa aaaacaaatc
     1981 agtttgattg ttcgaatttt caatttttta tgttctattt gttaattcct tcattttgta
     2041 agataatggg cacacgaaac aaaaaaggag cgatcgatga atgaacaaag aacgattgaa
     2101 acaagatttc tatgaagcag tcaatgaaga atggcaaaaa acagcaaaaa tccctgcaga
     2161 taaaccagct acaggcggtt tccaagattt agttgacggg atcgaccaac ttttgatggg
     2221 agaaacagac gatctgcttg cccatccaga aaaagtgtct tcagaaaaaa tgaaacattt
     2281 ccttgcatat tatcaactgg caaacgatta tgaccgaaga aatgagttgc atgctcaacc
     2341 gcttcaacct ataatcgaaa aagttgaatc attgcagtct ttcgacgaat tgaataagca
     2401 atttccagaa tggctgttgg atacattacc attgccattt aatctggatg tagatgcaga
     2461 tatgaagaat gcgcaaacga acgccttctt tgcttatccg ccttctttat tccttccaga
     2521 taagacgtat tatgcatcag accatcccaa cggtgcacaa ctaataggcg ttttctttga
     2581 tatggctgtt cagttattag agcttgccgg caaagaaaaa agtcaggcag aagcaatcac
     2641 ggaacaagca gtgcaatttg ataaattgat tgctcctcat gtaaaaagtg cagaagaaaa
     2701 cgcagattac agcaaaatgt ataatccgca atcttttgat acatttatta aatacacaga
     2761 taaactggac ttgaaaaaac ttgtcacggg tttgattggg acaactccag ataaagtgat
     2821 cgtaactgat ccagtgtact tcgatcattt gaatgacatc ctgacagaag ctcattttcc
     2881 gttattcaaa agctggatga tcattaaaac agtacgttct cttagtggtt atctatcaga
     2941 agaattccgc caagtcagcg gaatctattc tcgcgtattg tctggaacag acgaagcaat
     3001 gcctcagaaa aaagcagcgt attaccttgc ctctggacaa ttcgatcaag tagttggcga
     3061 ttactacgga aaaaaatatt ttggcgaagc tgctaaaaaa gacgtagaac aaatggtgaa
     3121 aaaaatgatc gccgtttata aaaaacgtct agaagaaaat gactggcttg gggatgcaac
     3181 aaaagaaaaa gccgtcataa aattaaataa cttaggtatc caagtaggct accccgataa
     3241 gatcccagca ttattcgacc agttcaaaac agtacctgca gatcagggtg gtactttgct
     3301 ttcaaatact cttgttttca gcaaactcac attaaaagat cgtttcagca aatggaacaa
     3361 accagtggat cgttcagaat gggaaatgag tgctgacacg gtcaatgctt attatcatcc
     3421 atttcggaat gtgatcgtct ttccggcagc gatccttcaa gcaccatttt acagtctaga
     3481 acaatcaagc tctgctaatt atggtggaat cggagctgtt atcgctcatg agatttccca
     3541 tgcatttgat aataacggcg ctttatttga cgaatatggc aacttgaaca actggtggac
     3601 agaagaagat cttacacaat ttcaaaacaa agcacaagca atgatcgaag aattcgatgg
     3661 tctgcctttt gctgacggaa aagtaaacgg aaaattaacc gtttcggaaa atattgctga
     3721 tgcaggcggt ctaagctgtg cattggaagc agcaaaaacg gaatcagatc tttctatcga
     3781 ggaattcttt gtcaattggg caacgatttg gcgtacaaaa gcaaaaaaag aataccaaca
     3841 acttttactg caaatcgatg tccatgcccc tgctaaactc cgagcaaacg tgcaaccaaa
     3901 aaaccttgac gaattctatg aaacatttgg tatcacggaa caagacgcta tgtatctagc
     3961 gccagaaaaa agagtgcgaa tctggtaaga ggttgtgcca gaagtggtca gcttcaagaa
     4021 ataagaagga atttgggaaa atcgcttctc aaatttttgt gacatttcgg cttatttttc
     4081 gaagaagtta cttctggaat accatgtatt agaggttgtg aaaaagctgc ttagtttcaa
     4141 gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt tgacttacta
     4201 ccgagaaact ggcttttgaa caccgtttat cgagaggttg tgaaatcagc gtcctgacct
     4261 gagtattaga gaaaaaaacg gaaaaatagt tcctcatatt ttgccgtttg ttgagctaat
     4321 accgagggtc agctgattga acaccgttta gtggagagat tgtgaaaaag ctgtcctgca
     4381 tcaagtaata agaacagcca atcaaaagca gcaaggagaa cctcatctat aaaaagatag
     4441 aaggaagtga gacatctatt ggtctcattt ccttctattt ttactttcct cagtcaattg
     4501 ttttacatca taaaaggaga aattccactt atttttttcg ctgtttcatg acataaacaa
     4561 ctacaccgat taacaaaatt cctccagcta atgctagcaa cgggttatta aattctcccg
     4621 tttttgggag tccttgagtg ccattttttt cactttcttt tgctgtccta tccggatgag
     4681 aaggttttcc atttggtttt ccatttggtg attcatttcc tggattttct ctttctggtt
     4741 tgtcatttcc cgggggcaca tcggcagttt ctcgcacagt gatcgctttt tgctgggtaa
     4801 cagattctgt atgagcagac catttttccc catcaaatat ttccgaacga taagtgactt
     4861 ctagcgtata ttctcctttt gaaggtgctg aaaaaatagc ggtatgatta gaaaattgag
     4921 cacttgactg tgtttcaacg atttggatct tttctgggaa ataacgcata tcttcaaaga
     4981 caggcgttcc ttgatttggt gtgaaagttg acacaaaaac tgtaaaaggc ttcagttcaa
     5041 tgatatttcc ttctgcattc tccgccacac catttgcttc ttgtataata atcggattat
     5101 tttgttcatt tggttttgtc tgatttggtt cttgtacgat tgcatactct ggatattccg
     5161 aagacggtga ggtttctccg tcaaaaatag cacctacaga taatggaaga ccatccactc
     5221 tttccttttc tgtttgacga ttccccggtt taatctgcat cagtccatga acatggatac
     5281 ttccattttc atagcgcgta acaggctgct gaccttcttt cgaacatgta tttagagact
     5341 cgatccaatc ttttgatatg gtatgtccaa gagtattttc tgattgtttc tctcgttcat
     5401 tccaatagta gttcaacgag tggtagattt gatcttcgtt ttgattcaat gggcggatct
     5461 gctctctcat ctcaagattc tctgttcgga aagataagat accagaagca tggtagtctg
     5521 tgtttcctgc tgaagaggta taaagagcca cattatatga accgttatta aaacttgtac
     5581 tgctattgac gataatatct gttccgctat tggaatcgat tcctttcgca agattttcga
     5641 aagacaaact attgatcaat tcatgagggc ccgttaaaga agaaccgccc attttgaatc
     5701 cattaccgtt accttctccc tcaattcctg gtacccagcc gtttcgataa gcaactgagt
     5761 ttttgatgat tacttttccg atcgaacctg tttcattttt agcaaaaagg tcccaaccgt
     5821 catctgcatt atgatacgcg atacaaccgt caaatacatt tccttctcct attgtcagtt
     5881 tggccgcaaa tccatcggca tcttcaaatc ctggatcggc attttcaaaa gatgtacagt
     5941 tcttgactaa gttatgcgcg ggccatgctt cgaaaggatc atttgagcta ccgctgattt
     6001 ggattcctgt atttccattc cgataagcat gaatctcttc gatcaaatta tgatttccag
     6061 aaatctgtaa tcctttttgc atagctgcac tatttgtaat gtcaaaccct cgtaaacccc
     6121 agtagtgact gaacagtgtc atcccatttc cagttccttt aaaatcaagt acaggacgct
     6181 tgttttctgg atttttctct gcaatcaact gaatcgggtt ttctcttgtc ccgttaacgc
     6241 cttttggaat cgtcaatcct ctttcgaagg aataagttcc aggcgccatg acgattgtct
     6301 gcccagcata tgcgtatctc aatgcttgtg tcagagataa tggattttgt ctgcttccgt
     6361 ttcctgcatc tgacccttct ggtgttacat aaactgtcga atatttaggt ttacggtagt
     6421 ccactgtatg aagaatctct tttgtttcat aactagtcat ctctacgtat tcttctggcg
     6481 gatagttttt atctggcgta aaactgaaag taaattcgtt cgttccttcc atcagttttg
     6541 tctgataatc aaattcccga ttcgcagtga tagccaagtt ttttgcgttc ttaagcacct
     6601 ctcctgttgc tttattttta attgtcaata atccgtcagc atttgctcta aaggacatag
     6661 tatgatcttg gtttcctgaa tgattactag aggtcacagt gtaaatcgga tcaatcaagc
     6721 gagtaggtct ttcttgccca ggaggatctg tcgcaggatc tgagtaagca acttcgattc
     6781 cttctacctt gatcgtcata ttccttgctg cagcaaatcc tgcataaata accgattcat
     6841 ctgaagcgta taatttttcc cagtcataca tgatgtcaga agccatttct tctcctgttt
     6901 caggatcgta ataatatgac tctaaaccag tattcgtttt tttcatctct acgtccagtt
     6961 tagtaatcga gttcgtggcc ggaaacaaat tcccatgacc gtttccgaat atattataag
     7021 aacctgtttc tttttccaag aaccctgctg aagtctctaa aggtgttgtt tccactgaga
     7081 cactcccagg agctggcgca gcttgcggat cttcagatga tattcctgtg attgcccgtg
     7141 tccccaatcc aagtcgcatc gtatatttgc tgccgctcgt gttgaccact tcttgtctat
     7201 ccgagtccca gacatattcg atccgactgc ctagtatggc aaatgaattt gaataaaatg
     7261 cctgcccaaa caaatgattt tttggtactg cgtctcttac cattaaagca aatccttctt
     7321 gggcattcgt ataggtccaa gattctattt cgatcgtcgc ccgcatccta aagttgatgt
     7381 ctttggggat tgcttgataa taataggtca ttccatctga tccgcttgac tggaattttc
     7441 cgccgttatt cgtggaagag agccgcacgc cgtcttctaa tggttcaatg gcatacctac
     7501 tagcacttgt gctttctccg aaaacaaccc ctttccactc ttcatttgct tcttcagatt
     7561 gcttttttgt cttttcattt tctgattctt tcatcatagg atcatttcct tcttccgtat
     7621 ccatatcttc actagttctt ggtacttcct ccattgttga catcccttca atgttcacgt
     7681 gtgcattttc ttcgttcaca gactgttgtt cgttagcagc aacttcaacg acagaaatta
     7741 aaaccggggt actgagcata aggagattca tcattagcgc actgtatttt atacgttttt
     7801 ttaacaagtg tattcctccc gtaacaaatt agaaagcgtt tacttaaagt attacctaat
     7861 ttatttttac tgtcaataaa aaacaaaatt ataata
//
LOCUS       DARBEC010000111         7613 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.116, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000111 DARBEC010000000
VERSION     DARBEC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7613)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7613)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7613
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.116"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..790)
                     /locus_tag="SCL80_002506"
     CDS             complement(62..790)
                     /locus_tag="SCL80_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108344.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(803..2083)
                     /locus_tag="SCL80_002507"
     CDS             complement(803..2083)
                     /locus_tag="SCL80_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1108345.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(2224..2826)
                     /locus_tag="SCL80_002508"
     CDS             complement(2224..2826)
                     /locus_tag="SCL80_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEG1108346.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            3071..3277
                     /locus_tag="SCL80_002509"
     CDS             3071..3277
                     /locus_tag="SCL80_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108347.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            3589..4308
                     /locus_tag="SCL80_002510"
     CDS             3589..4308
                     /locus_tag="SCL80_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEG1108348.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
     gene            complement(4389..5072)
                     /locus_tag="SCL80_002511"
     CDS             complement(4389..5072)
                     /locus_tag="SCL80_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEG1108349.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
     gene            5290..7476
                     /locus_tag="SCL80_002512"
     CDS             5290..7476
                     /locus_tag="SCL80_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1108350.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
BASE COUNT         2729 a         1283 c         1432 g         2169 t
ORIGIN      
        1 aaaagaaccg aagggtttgg atcggctggt catttagtcg gtccaaacta ttcggttctt
       61 tttatgaaat caatttcttt aatccacgga tagaggctgg gatggtctcg ccgtttgcca
      121 acgtgacttc tctggttttt ttattaatcg accgcacatt ttgtgtattg ataacatagg
      181 atttatgaca acggataaaa ttccgatgca ttttctcgat ttctttgatc ttctcataga
      241 attgtaattg ggcatgagtc aaatggactt ccagtttatg tggcgctcct agcgaagtag
      301 aaaaatattg gatgtctttg atcggtaaaa atttgatctc atcattaaac tctattttga
      361 agatatcttt ttggatttgg gacatttgaa aatcttcgaa aattttgctc aggatatgct
      421 cgacattttc ttttaaatca taaagatctt ctttgacgat ataatcagtc ggttcgatgt
      481 tgctttttaa gattttcatc gacatttccg tatgggaagt cacatagatt atttttccta
      541 aaggatctaa ttcacggatc aatttaccga tcgctacacc atccatttca ctgtcttcta
      601 gttcgatatc taaaaagtaa ataccaaaag aagaagttct gatcttggct gcattgagaa
      661 gttcttccgg atcagaggta gaaatatgga cataactgtc taagttttca atcatgattc
      721 tatttttgat caccgttgat aaaacttcta attgttttgg atcgtcttca caaatataaa
      781 tgggaatcat atcattactc ctttatttta tttgtatata aatcgtttga gaaaaacgct
      841 ggttttcaat tgatgtttca aggaaaagat aaggattggt ctttgtcaat tcatccaaga
      901 taaacaagcc aaggccttcg ttttttggat ttgtttttgt tgagaaccct ttttgtttca
      961 actggtttaa cggtaatgta tttgttgttg tattgatcac acgaatcatc acggaatgat
     1021 tcttcttgag tatggtcaat tcgatttttt tcttctttgc agcactagct ccttcaatcg
     1081 cattgtccaa gataatacca agcatacgta caaggtttac cgtatgtttc gtatccagtt
     1141 cgattggctc aggaaaatga agctggacga tcaacccttt ttcctgtgcc atcatgagtt
     1201 ttaatgacag taaactacgg atctcaggaa ctttcatatt atccaacata cttagattgg
     1261 ccggaaaaag gttcatcatc tggcgtgtag gtaatatcgt ttgatcataa taagaccgta
     1321 atccttctaa gtcgccttct gaaatatagc tgtgaagaga aaaaagcaga ttttggtaat
     1381 catgtttgaa ttctctcaag tcttgatagt ttttttctag atctttgata taatcctgtt
     1441 gcatcctgac ttgttgctct aataactgat cagctaattt cttattagag attttccaat
     1501 aataaaccaa gatgatcagg ccaacacaca gatatagaat gaaaaaactc gtattcaaag
     1561 ctaagacatc aggtgtttca ccagaaaaac gggtataaaa aataatgata tagtatgtaa
     1621 ataatatcaa tgtcccgatc gtaccgatca tacggcggtc cacagtgtga aaccaagtct
     1681 ctaacgattt ttttaaacaa aaagctaata aagcagataa aaaagcggat aagacaatgt
     1741 gaaaatactg caatgaatct cctggttcaa taaccgaaga tttcaaaaca aaaaaatcgt
     1801 cgatgcttgt taagtgatct cctaaaagcg acgtcaataa cccaaaagga agcgcgtaaa
     1861 ccaattgttg acgcgaagtc gaaaagaaga aaaaagtaag catcagcatt cctatagcaa
     1921 ttagagcagt aaaaatccct aaaatcgaat agacccatcc aatcaaaata caagaaatca
     1981 gaatgactcc cgcaaacaga tatttgtatc ggatgtgatg gtcaatcaat aagatacaaa
     2041 ttgtgtaaat caaatattgc aacgctatgg accataaaaa cataaagtac agctcccatt
     2101 gatttttacc aaattatagc acaattagcg tattattttt ttaatttttc agggattttg
     2161 aattcagaac tcataccaaa gcagcagcca gatacaaaaa agtcagataa gttagttaat
     2221 tgtttaatga ttgttttttt catgaaagat tcctccaaaa ttcatcaagt agtttgtaat
     2281 aggaagcaac gtagctattt ctaaaacaat cccaagtagc aaataggtag aaaagtaaga
     2341 agctgagaaa aataaagtaa tgagagaaaa aattagaaac atgctgatcg ccttctttaa
     2401 taatggggga cgtttctcgg gttcaatggg attgattgct gtacctcttg atccatagcg
     2461 aagcagtaac aaaaagcagc tgattaacaa aaagaccatc attgggaagg agatagtcaa
     2521 ttgcagatgt ttcatcacca agggaacgcc ccataaataa agtagtgtcc acagcagaca
     2581 aacgaattca gagtcggaat gcaatccata agcatatctg cggatgcaga tataagaaag
     2641 aaaggtgatt cctgtttccg cccatgagcc tgtcaccaga gaaaagacta gaacgaccgc
     2701 tagttttcca agattattca ataaaacttc taaggcaaat tttacttgga catacaccat
     2761 atcttcttcc gatttgtctc cgctgaataa ggcttctaat aatttgttca caactttatc
     2821 tttcatctca tacgtacctc caccacaaat tttatgctaa aaaaaataaa aaacgtcttt
     2881 tgtttcataa aaagtggttt tgacatcaaa accgtcatga tctcctaaaa aaatcttcgt
     2941 tttccaagaa ccttaccaaa aaaagtgaca gttttgatac gaaaagtact ttttatgaca
     3001 agtttgtgct ttttcgagtg atttatgtta aaaaattact gaccttataa aaggtgatgg
     3061 agggattctt atggaaaatg aacaattgtt tgaagtcggc gaaacatatg catttaaatc
     3121 tgatttattt gaatcaccaa taaaaggaaa aatagaaaaa atctatgaaa atagtgtgat
     3181 gatacgtgtc atgaaatgcg cagtagaaga caatgatacc gctcataatc tgcactggca
     3241 gttagttgta cgcaaatcaa gtacaataga agaataaaaa cagataaaat gttgagagca
     3301 taaagaggtt gtgagtctcc cgtttagctc tgagttacaa ggaataatgt taaaaaacag
     3361 cttttcatgt tttatgaata ttgccttgtg accgaggtgt tggcaggaga atactgttta
     3421 tttgaaaaaa gggctgcgac aagacttttg tcacagcctt tattttcatg aataggagtt
     3481 acttttggct acttttagag aagtagcttt taaggagcag ataaagtcaa ggcttttatt
     3541 tctataaatg aagtagtaaa atgaagaaca gattttatta caggaaggat gccgataatt
     3601 gtgatcattg ttgcaggaac gatcggtgca gggaaaagca cactaactga aatgcttgcc
     3661 caagatttag aaacaaaacc tttttatgag aacgtggaag ataatgaagt attgccgcta
     3721 ttttattcta atccagaaaa gtatactttt ttattacaga ttttcttttt gaacaaacgc
     3781 tttttggcaa tcaaagatgc tttcagccat gatgacaacg tactagaccg ctccatctac
     3841 gaagatagta tgctgtttca tttgaatgca gatttagggc gtgtttctga agtagaagta
     3901 aagcagtatg aaggtctttt agaaacgatg ctgaaggagt tagaagaaat ttcgccacaa
     3961 aagaaaccag atctactggt ttatattcgt gtatcctttg aaactatgtt agcgagaatc
     4021 aaaaaaagag gccgggaata tgaacagcta gaacaagatc ctgaactcta ttcctactat
     4081 aaagaactca atcgacgata cgaagagtgg tatgaacagt ttgatatatg tcccaaaata
     4141 gtgatcgatg gggacaaata tgatttcgta gccgatccgg catgtggaga acaaatcgta
     4201 caagaaatca agatgcgggc aaagaaaatg acagaagagg cagatgccca gtcgatccgc
     4261 tacactacaa ataacccgaa agcaaaggga atctttacaa ataaataata taaaaactaa
     4321 gcaaattgct cactcgcaga tatttctctg tgagcagcat cttgcttagt ttttttgatt
     4381 cattgagatc aaatagtgaa ccgattcgta tgataaaatt gccataaatg aaatgtctga
     4441 gtgagggcca tgatcaaaaa taaaatggaa acgatcaaaa tcgaatgggc gccaaacaga
     4501 tgatgacaga aaacaaggac aatagctcct gcgataaagg caagaacgac acttagatag
     4561 tggaaaaatc gatcaaaatc tgctttttct ttagaaatac aataatcata taagttcgtt
     4621 gctacccctt ttaaatttcc agttgtaaat aaattagtgt aggcacgccc gttgatttta
     4681 tcaaaagcta cccactggat cgatgcagta aaagcaatca aaatcgttac taatgcggga
     4741 tgctcatgga cgggtgtcaa actaacaaag aaaaaaatca ctgcttcata atatagaaca
     4801 aacaagcgcc agaaatgttt ttcttttttt ctaaaataat caattagata ctttgttaga
     4861 agaatcccta agaaaaagaa aagggtagac aatacttttt taccaacact tagccattct
     4921 ccgtcaaatg cttggatgat cgatagaatg atattgccag tttgagcaga ggcaaaagca
     4981 ccatattgga tataagtata actgtccatg gctcctccga taaatgtcag caaaagtcct
     5041 attgtacgcc cctcgtgaaa ggaagtctgc atttttttca tcacctttta tgaatatttt
     5101 tgtcttcctt atcttagcat aaacgattga aaaaaaggaa aaattttcag aaaactaaaa
     5161 tcatatcgct tcccattttc atttttagaa aggtctgtta gtataaagaa aaaatagaca
     5221 acgaaacttt aaataaccaa cttgaaataa acaaattcaa agaagaaaga tgggaaggaa
     5281 atcaatcagt tgaatgaaaa agaaattctc cgattacaaa aagaattgaa acgctgggag
     5341 aacaagtata aaaagcttca acaagtcttt cggctacaag aagaaatcat tgctacctat
     5401 gaagcggctt atggcgaagc acccggactt attgaggaag aagtacaagt aaaagaaagt
     5461 gaaggaaaca gctctttctt taaaatgatg gagaaacgtt cctataatcg cttcaatcaa
     5521 gaacaaaaag cagctatcac atatgatatg aagaaacatc ttcgaatcat agcaggagct
     5581 gggagcggca aaacgcaaac aatctgtgca aaagctgttt atctgatgac gcaaaaacaa
     5641 gtagacgaag aacgcatact aatgatcact tttacaagga acgctgcaaa tgaactaaaa
     5701 aaacgagtag ataatttcag ccagagaaaa accgctgtcc atatcggaac attccatagt
     5761 atctttttta ggatatacaa tgaaatttgt cgaaaatttc ctgaagtagc aatgcaagga
     5821 atacaaggag acttttcaaa ggatactgca caaaaagtga atgctgtttt gcaacagctg
     5881 atacgtaaat ataatcttta tatatttgat caatatggag aaaaaacgat tgcttctcgg
     5941 ttagactatt ggcagaatat gaacctttca gtagaagaaa tggttcagtt agttaaagaa
     6001 aaatatgatt ccattgataa gaatgcacga cagccgatat cagaacgact gtatggattg
     6061 ctcacggaac tccaagaaca aaaaagaagc cagcaattgc tagagttcaa tgatgtactc
     6121 caaaatctga aaagcgcttt ggagaatgag gagatccagc attatatcgg ccaaaaatat
     6181 gattatctat tcattgatga atttcaagat actaatccac ttcaatggga aatcgtgaaa
     6241 cggatgacaa aggaaaacag gatcaagttg attgttgttg gagatgatga tcagagtatc
     6301 tatggattca gaggatcaga gccagcatac atcaagaact ttgaaaaaga atatcctacc
     6361 aaaactcttt tcttattgac taattatcgt tctcgggcag ctattgtcca aggagcaaat
     6421 agattgatca gctataataa aaatgatcgg ataccaaagg cgatgattcc agcacaaaaa
     6481 gaagcaggtg ttatggaagc ctatttgttt tctgatacta aagcagaaag tcagtggcta
     6541 atcaaacaaa ttcagacatt tattcaaaaa aatgggaaat ataaggaatc aatcatatta
     6601 taccgatcag cttcacaaac gcagcagctt gtccaatctc tgctgaaaac gaatattcct
     6661 tttgtactgg aagcagatag tccatatgaa ggtatctttg gtatcaagtc tttccaacat
     6721 ttctatcaaa aaattgttca ttggcaaaca gcactgaatc tgcaaaagaa aaatcaggcc
     6781 taccagcaat tgctccggca gctgatggca gactgctacc tgaaaaaaag tgaatgcgat
     6841 caatactttt ctcctaaata tcaaaatata gccgttgcag attatatttt gtcgataagc
     6901 cccaatttaa aatcgaaatc tgcagaaatc cgagagtttg aggaagcgtt gcgtaaaaca
     6961 gaccagcatc aagaaatcta ttcgcttgtt caaagctatc ttcatctgcc acgtatagct
     7021 aaggaaatcg accagagtga tcaagaatgg atacgagaag aagtaaagca gcatgctact
     7081 ttcagaagac tccaagcctt atcgcaagaa acccaagcga cagctaaaga attaaaaacc
     7141 cgcttgattg cttacagaca aggaaaactt gatgcactat gtatccagag cattcacaaa
     7201 agtaaaggtc ttagttaccg aaatgtcttt ttgattggtt gtaacgaagg aagtttgcct
     7261 tataacggag cgacagaaaa gaaagtaatc gactttaatg aaataaaagc agaaccagtg
     7321 acgacgatag aagaagaacg aagactattt tatgttgcga tgactagagc aagaaatcgt
     7381 ctgtatctat gtgtacctca aatgaaaaat tcaagaaaat tgaaagtatc gagatttatc
     7441 aaagaaaccg ggagtcgtgt gaaaaaagga aagtgataga agagagggtg tgacagaagt
     7501 ggtcagtttc aagaaagaag aaggaatgta cgaaaattgc ttctcaaatt tttgtgaaat
     7561 ttcagcttat tttcgaagaa gctatttctg gaacacagtt tattcggaaa agg
//
LOCUS       DARBEC010000112         7326 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.117, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000112 DARBEC010000000
VERSION     DARBEC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7326)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7326)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7326
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.117"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..654
                     /locus_tag="SCL80_002513"
     CDS             <1..654
                     /locus_tag="SCL80_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108351.1"
                     /translation="NLVNRKSTTKFANSFWFLKNINKTVEKNGVYKGDFHFQNAETIE
                     REHHYFDLLVNTVVELAKWAKSNETYSQVLKRIMSQETSFIYELSKSNLKYTYNEDEL
                     EILDNPENSQWIKELKREDFIETLRILKSLFDLKLAKATIMGIERDSYYWEEIGLYAP
                     QIEGEDKEKRIDNIINYINSLSEPLKCRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            837..3512
                     /locus_tag="SCL80_002514"
     CDS             837..3512
                     /locus_tag="SCL80_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEG1108352.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGITKIEATFVEAQKKLWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            3694..3933
                     /locus_tag="SCL80_002515"
     CDS             3694..3933
                     /locus_tag="SCL80_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108353.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLDFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNRRVVKKWYLVHS"
     gene            3949..5322
                     /locus_tag="SCL80_002516"
     CDS             3949..5322
                     /locus_tag="SCL80_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108354.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGEKAERPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLSYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEVHEARIVRYIFQLYLTKKYGYKKLCQRLTQQKLFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKATFLQAQEIRQSRCTAKKDTYLYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKRKVIQQKKQEAQWIKAFEEGNCSLEELKQQLQALQ
                     KPMSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDYVCRIEVTKDKTVCGIYFRQLTQ
                     NILERNE"
     gene            5326..6960
                     /locus_tag="SCL80_002517"
     CDS             5326..6960
                     /locus_tag="SCL80_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108355.1"
                     /translation="MEMIQTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMGYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPRPILVKGTHEPIISEEDY
                     QKVQERLALEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQGEPIQVKDVQRILTNLDRFLAQSEKKQIKALYRTFIEKITFDPNHKEMLEITMK
                     FTHAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            7123..>7326
                     /locus_tag="SCL80_002518"
     CDS             7123..>7326
                     /locus_tag="SCL80_002518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002395661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108356.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTG"
BASE COUNT         2616 a         1141 c         1454 g         2115 t
ORIGIN      
        1 aacttagtta atcgaaaaag tacgactaaa tttgcaaact cattctggtt tttaaaaaat
       61 attaataaaa cagtggaaaa gaatggagtt tataaaggtg attttcattt tcaaaatgct
      121 gagactattg aacgagaaca tcattatttc gatttattag tgaatacagt ggtagaatta
      181 gcgaaatggg caaagtcaaa cgaaacttat agtcaagtac ttaaaagaat tatgagtcaa
      241 gaaacttcat ttatctatga gttaagcaag tcaaatttga aatatactta taatgaagat
      301 gaacttgaaa ttttagataa tccagaaaat tcacaatgga ttaaagagtt gaagagagaa
      361 gactttattg agacactaag aattctaaaa tcgttatttg atttgaaatt agccaaagct
      421 accatcatgg gaattgaaag agattcatat tattgggagg aaattggact atatgctccc
      481 cagattgaag gtgaagataa agagaaaagg atagacaaca ttattaacta tattaatagt
      541 ttaagtgaac ctttaaaatg tagaggactt gattttatag gaacattagg atggacagaa
      601 acatactatg agaaaatata taaagaaata attgaacaat attttcctaa ataatttaat
      661 aaggattgat ttatcgtaaa agaaaaaagt tgtgatttcc aaaatcacaa cttttttctt
      721 ttgtcaagat tttgagtggg atatattttt gtagaactga agatcatgaa attatgagag
      781 gttggaggta atgtagtcat acaaataaaa attttttaga aagtgagtgg taattaatga
      841 aaatgaatca cacttcaatt cagaagcatt tacaactgtt aaatacactg gggatttctt
      901 atgttaattt gagaactttt tctacattaa acaagaaaac cgtttctcat attttttcta
      961 ctgcagattc tcaagcaatt attaactatt tagaaaaaca gaaattgtct gaaggtacag
     1021 taaacataac ttataatcct attaaacaag aggtgattac acaaaaaaag cattctgtga
     1081 gagacaatga tattgaaaaa attcggtttg tatttattga tattgaccct aaaaggaaag
     1141 agggaagtgc aacagatagc gaaaagaaaa aggcagaaaa tgttatggaa caagtagaac
     1201 gttatttgaa agaaaagaga atagaatcat ttgtaaaagt tgatagtggg aatggctacc
     1261 atatttttct tcccataaat gaacaaccaa ataatcacga gacgattctt actattcaga
     1321 attttcttca attacttcac cgaagatttg gagtagaaga tgaagttgat attgatactt
     1381 ctgtttataa tccttcaaga ttatgcaaat tagttggtac accagcggtc aaaggaaagt
     1441 ccacaaaaga gcgtccgcat cgtttatcta gtttcctttc tattcccgat aacatagata
     1501 gaagtaattg ttttaaaatt cttgaacaga tagttgaaga aaattccatg atgtcaacct
     1561 ctagagtaaa aagccatcca tcttttagag aagtgttacc ttttattcaa gcagactata
     1621 aacaatggtt agctcactat cctgaattaa cttataccat aaaagagggc gatcataaag
     1681 gagttacctt acttatattt gacgaatgtc cattaagaaa acacacgaat aattctaatg
     1741 gttcttgttt gtctgttacg gctgataata agattcgatt tcattgtctg catgcttcgg
     1801 ataaaaataa agatattcaa gactttgtaa aaaaatatcc gttgccaaaa cctgtaaatc
     1861 cttctgtaga atcattagaa tccaatcaag aatattattt tgaccagttt aaactgaatc
     1921 aagaaggagt atttttggtg aaagaaggtc aagaagaaaa agttgcttgt ccgatgttta
     1981 ttaagcaaat acgcaaaaat atcgaacaaa aagtcattac acttgttctt gcctattatg
     2041 tcgatcattg ttgggatgaa atagaaatag aaggtggatg tttacaaact aataatttta
     2101 aacaattagc aaaacatggc ttaatcatta aagctagaag agaagctgaa gtcgttgact
     2161 ttttacagat tcaacgggag aatgctccaa tcttttttga acatgaatct cttggctgga
     2221 aaaaagaaaa tgaagcaatg atttttcagt tacaagattc ttttggacgt gaaattcctt
     2281 ctgtattaac taaaaactca tgctaccaat taacaactaa tggtacagaa gcggaatttg
     2341 acgcattggt tcataagtat gttttaggaa cgaacttaga acttgcttgg tcgattggta
     2401 ttgcttctat tgtgttaggc tatttaaacg ttacgaaggc taatactttt ttgagtttac
     2461 taattaacct acagggcaag agcacaacgg gtaaaaccac tacgttacac tttattgcct
     2521 ctttatttgg aaatccaact acggttctta gaaatatgaa tgctaccaat aaagccatta
     2581 taaagctagc atcaaacaat cagggaggtg ttccactaat actagatgag ttaggagcag
     2641 caactcaaaa taactttagc tcgttgatct atcaattagc ttcaggagaa gaaaggctaa
     2701 gattagattc taattgtcag cttatgccac aagagacttt ttctgttttg acccttacct
     2761 cttctgaaga gcgtttggaa aattatttca atgagaaatt aactggtttg aaagttcgat
     2821 atatcgaatt ttctgatatc aagtttacaa aggatgcgaa tcacgcagag aaagttaaaa
     2881 gtctttcttc taatactttt ggttggggta taaagttatt tcttttgaaa ctctttgaac
     2941 aagggattac aaaaattgaa gcaacttttg tcgaagctca aaaaaaacta tgggctgttt
     3001 tacctgaaca ttctttgaag caacgcattg gtaataatct agctttgatt tatagtggag
     3061 ccctcctaat tcaagatata ctgggctatc ctttacgatt agagcatata cagacggtgc
     3121 ttattaattc gtacgaacag ttaattgtag atactagttt aggaagtgag caaacggtat
     3181 ttgaccgagt gaaaacattg ttggtgatga atagccataa atttttaaca aacagtgaca
     3241 acaaacatac gcccagtacg atttggggaa aagtatcaat tcaaaaaaat ggggatatta
     3301 aggtaaatat ttttcctatc ccgtttgagg acttgttaag gcgagaattt caagtaaagg
     3361 atctaaccta tctttttagg caattgcttg attcgggtaa tatgtgtgca gaaaaaggga
     3421 gaagaacgaa acgtattcac atgaatcgtc aagttcttcc aacatacgag attctattac
     3481 cttcttcact caaatcgtat tttcgattgt aatttagaat aaaccaaagt atgaaagtga
     3541 ggcaccatgt tatggaaata tttcttattg atttagcaaa acaactgatt acttatctgt
     3601 atgatagcga gcaaacccaa acggaagtaa atgaagcttg atctatgctt catttacttc
     3661 attcaatcaa acatgaaaga aaggaaaatt ctaatgacta aaaataaaat gacactaaaa
     3721 gcagaggttc ttctgtatat tcaagaacat ttttcaaatc aagccttttt tacaaaacca
     3781 atttacttgg atttcgaaat aagaggggta tctgctggat ctatcggggg tactcttcaa
     3841 gcgttgaaga acgaaggata tttagaaaat cactttgtgc aacgttcgtt caatagaaga
     3901 gtcgtgaaaa aatggtattt agttcattca tagagagggg aatcaagcat gcgtgtggct
     3961 tgttatgatc gaacgtctgt ttatgaagaa gcgaaaatta gtaatgacgc tcaatatgat
     4021 gcagtgaaag agtatgtttt agagaaatat gaacaaattc cccaacgatt ttcagacaaa
     4081 ggagaatcag gggaaaaagc ggagcgtcca gcttttcgac agcttagagc ttgggtttgt
     4141 gcgtcagaag aagagggggt gatctatgtt tggcgttatg atcgattggc acgtcaagcc
     4201 aaaatagcct tagaatttat tgagttgtgc caacaacatc aggttcaagt gtattcgatt
     4261 tctgaacctc ttcctgcttc tttctcacac gcgtcaacga ataaactatt tgtgcaatta
     4321 ttgttcactt ttgcagaaca acaacgaaac gtcattattg agaatgttcg tgccggactt
     4381 tcctataaaa agcaacaacg gcaatatttg tcttctgcgg taccatatgg gtatcgttta
     4441 gtgaatggga aaatccaaca agaagtacac gaagcaagga ttgttcgcta tatttttcaa
     4501 ctttatttga ccaaaaaata tggctacaaa aagctttgtc aacgattgac gcaacaaaag
     4561 cttttcttcc gcgaacgtcc ttttcagccc tatcatattt atagtatcct aaaaaatcca
     4621 ttgtactatg gcgaaatcaa aggaggatct ttggggaagt atctaggaac ctttgaaccg
     4681 attctctcga aagcaacctt tttacaagcc caagagattc gccaatcaag gtgtacagct
     4741 aaaaaagata cgtatctgta cttattacgt caaaaaattc ggtgtccctt ctgtggtcgt
     4801 catctgagtt cgaagtatca atggaataca aagaagacga agacgcttca ttattatcat
     4861 tgtaccgacc gaagctgcca aggaattttt cttcgggcag aaagggttga gtcagaagta
     4921 ttggaaaaga tacaggcttt ttttcaatac gaaggcgtct atcaagcctt gattgcccaa
     4981 ttaacgcaac aattagccga aaagcgaaaa gatgaaaaac gcaaagtaat ccaacaaaag
     5041 aaacaagaag cacaatggat taaggcattt gaagagggca attgttcact ggaagaactc
     5101 aagcaacaac tgcaagctct tcaaaagccg atgtctactt cttcgtttct ttcactccaa
     5161 cagtgtgaag caaaagtgaa agaatggctt caactaaaag acctctctag tcgacaatta
     5221 ttatgtgact atgtttgtcg gattgaggta acaaaggata aaacagtttg tgggatttac
     5281 ttccgacaac tgacacaaaa tattttagaa aggaacgaat gatctatgga aatgatacaa
     5341 accaaacgag tcgcattgta tattcgcgtg agtacacaag aacaagcttt agaaggacat
     5401 agtattcaag cacaagaaca aaatggacga cagtttgccg aacggatggg ctatgaagtg
     5461 gtagaagttt atgccgatga aggaatcagt gggaaatcca caaaaaatcg gttcgcctat
     5521 caacgaatga tgaacgatgc tcggaaccat cgttttgatc tactagtgat ttggaaattg
     5581 acccgtctcg gacgcaatat gttagatgtc ttacaaacag tcgaggaatt attgcgatat
     5641 gacgtaggat tacattcgat ctcggaacaa tttgacgtga ccacttcgac aggtaaattg
     5701 atgttacaac tccttgggag tttcggtgaa tttgaacgca accaaattgc ggagaatgtt
     5761 caaatgtcaa tgcaaagctt agtgaaagat aaaaagcgtt atgcaggagg ccgacgttta
     5821 ggctatgtca gtggtattga cacgaatggt caaaaacaat tggtcattga accagaagag
     5881 gcgaaaattg ttcgtctgat ctatgcaaaa tatttaggcg gagcagggta tcgagcgatt
     5941 gccaattttt tgaatcgtca aggatatcaa acagtcaaaa agaatgcttt ttctaccacg
     6001 gcagtcaaag atatcttacg caataaaatt tatgcagggt atttagaata tgcgcgctac
     6061 atcaattggg aaaccaaacg acggaagggg aagaatccga gaccgatttt agtgaaagga
     6121 acccatgaac cgattatttc ggaagaagat tatcaaaaag tacaagaacg ccttgcttta
     6181 gaaggaaaac aacccaaatg gaaccataca ggtgaaaatg tattgacagg cttgttacgt
     6241 tgtccagaat gtggcgcacc gatggcagcg agtaatgtca caaataccct aaaggatggc
     6301 acaaagaaac ggattcgata ttattcttgt tcagtctttc ggaataaagg agcaagtgtg
     6361 tgtcatgcca attcgattcg tgccgaacaa gcggaaacat ttgtcgccga ccgattaaag
     6421 gagatcgttc agctaccaga agttcttccc cgattggtgg cagcgttaaa tgaagaaata
     6481 gtgcgacaaa gccaaccatt ggaacaagaa ctcgtggtgt tactggaacg aaaggaagaa
     6541 ttgaagacta aaattgaaaa atgggaagct gctttggaag atagtcctga attgtttccg
     6601 atgttaaaag accgcttaga cgaattgact gaaaaacgac gacagttgca cattcgagaa
     6661 aatgaaatct tagggatctt tcaacaagga gagccgattc aagtcaaaga tgtccaacgg
     6721 atattgacta atttggatcg ttttcttgcc cagagtgaga agaagcaaat caaagccttg
     6781 tatcgtacct ttatagagaa aataaccttt gatcctaacc ataaggagat gttggaaatt
     6841 acgatgaaat tcacgcatgc cgtggtacaa caattgaatg agcaatatca aatagccgta
     6901 tccaaaacaa aggatacggc tatttttgtg ttaaaaacac cctttacgtt aacgatctag
     6961 tctaggttat atcaaacaac gttagagggg cgctaacgct tctctagcga tttgaaatcg
     7021 ttagagaagt agttagggag gtccttatca taaataggga tatttattaa tgaatatttg
     7081 aaatataatt gtatataaga attaaaaaga gtggggatag atatgaaaaa gatgggtaag
     7141 tgtattttat gtgaaaaata cactgaactt actaaagaac atgtaccacc taaaaaatca
     7201 gggaatactg gaggtaaaaa gaccagaact ggtaatttag atgatttcct taagagcgat
     7261 tttacaaaag gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa tgtttattat
     7321 actgga
//
LOCUS       DARBEC010000113         7308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.118, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000113 DARBEC010000000
VERSION     DARBEC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.118"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            35..532
                     /locus_tag="SCL80_002519"
     CDS             35..532
                     /locus_tag="SCL80_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108357.1"
                     /translation="MNNQITLAYLDYDDLDALQVVSVVSFYESFIDGADPLDMQGYLQ
                     TQLTTEILAVELAQSTSKFIGIKDHQILIGYMKVNDEKDAIEIQRLYLLKDYQNKGLG
                     QRLLDEANRYAKTKQKRYLRLTVYEKNHAGIRFYERHGFKKIGIKHFPLGKQDRICPI
                     LEKEI"
     gene            complement(515..1252)
                     /locus_tag="SCL80_002520"
     CDS             complement(515..1252)
                     /locus_tag="SCL80_002520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108358.1"
                     /translation="MTTEHFDLANPVTKVDDIPDYEMYSQTIDSLNKRFGNRVLKGIE
                     IGYIASEKDRIIDYLADKDYDLKLLSVHHNGQFDYLDDEVKDMDPAIVIPQYFAQLSE
                     ALVVIEADVFAHFDYGFRVFGLSVAEFKQYEAQFLPILDQVIKNKLAFELNAKSAYLY
                     DNLALYEYVIDLYLSRGGTLFSVGSDGHYLEHFRFHFDDLFALLKAKGVTELAIYQKG
                     KRIMVPLPVQKNVLSIKSEDVFLDFFF"
     gene            complement(1444..2622)
                     /locus_tag="SCL80_002521"
     CDS             complement(1444..2622)
                     /locus_tag="SCL80_002521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfm2 family transposase"
                     /protein_id="HEG1108359.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKVRTLNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDIYSRG
                     VHDVLLFITDGLKGMKDTIHQIYPKAKYQHCCVHVSRNIAHKVRVKDRKEICDDFKAV
                     YQASSKEEANTFLGSMIEKWQKTYPKVTQSLIKNQDLLTFYEFPPGIRRSIYSTNLIE
                     SFNKQIKKYSRRKEQFQNEESLERFLVSIFDTYNQKFLNRSHKGFQQVTDTLASMFTE
                     "
     gene            2789..4828
                     /locus_tag="SCL80_002522"
     CDS             2789..4828
                     /locus_tag="SCL80_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEG1108360.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAASTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKMITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLITPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(4937..6088)
                     /locus_tag="SCL80_002523"
     CDS             complement(4937..6088)
                     /locus_tag="SCL80_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1108361.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(6242..6772)
                     /locus_tag="SCL80_002524"
     CDS             complement(6242..6772)
                     /locus_tag="SCL80_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEG1108362.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(6871..7221)
                     /locus_tag="SCL80_002525"
     CDS             complement(6871..7221)
                     /locus_tag="SCL80_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEG1108363.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
BASE COUNT         2529 a         1352 c         1339 g         2088 t
ORIGIN      
        1 atttatagta tactatgtca aaaggaggag ttagatgaac aatcaaatca cactagcgta
       61 tttagattat gatgatctag atgctttaca agttgtaagt gttgtcagtt tttatgaatc
      121 gtttatcgat ggcgcagatc cacttgatat gcaaggatat ttacagacac aattgacaac
      181 tgaaatattg gcagtagaat tggcacagtc aaccagtaaa tttattggta ttaaggatca
      241 tcaaatactc attggttata tgaaagtcaa tgatgaaaaa gatgctatag agattcaacg
      301 gctctatctg ctcaaagatt atcaaaacaa agggttaggg caacggttac tcgatgaagc
      361 gaatcgttat gctaaaacga aacaaaaacg ttatttacgt ttgactgttt atgaaaaaaa
      421 tcatgcaggt attcgttttt atgaacgtca tggatttaaa aaaatcggga tcaaacattt
      481 tcctttagga aaacaagatc gtatttgtcc gattttagaa aaagaaatct aaaaaaacgt
      541 cctcactttt gatgctgagg acgttttttt ggactggtaa tggaaccata attcgctttc
      601 ctttttgata aatcgctaat tccgtcacac cttttgcttt taacagagca aaaagatcat
      661 cgaaatggaa acggaaatgt tcaaggtaat gcccatctga tccaacacta aacaaggtac
      721 caccacgtga aagatataaa tcgatgacat attcatataa cgctaaattg tcatacaagt
      781 aagcgctctt agcatttaat tcgaatgcca atttattttt aattacttga tctagaatcg
      841 gtaaaaactg cgcttcatat tgtttaaatt cagcaacgga tagaccaaat acccgaaaac
      901 catagtcaaa atgagcaaag acatctgctt ctataacaac tagtgcttct gaaagttgtg
      961 caaaatactg aggaatcaca atagcaggat ccatatcttt gacttcgtca tctaaataat
     1021 caaattgacc attatgatgc acactgagta attttaaatc gtaatcttta tcggctaaat
     1081 agtcgatgat ccgatccttt tcagaagcga tgtaaccgat ttcgatgcct tttaaaacac
     1141 gattgccaaa acgcttattt aagctatcga ttgtttgact gtacatttca taatcgggga
     1201 tatcatcgac tttagtaaca ggattggcta agtcaaaatg ttcagttgtc acgacgtaac
     1261 catcgtagtt tgttaaatag tcctcatttc ttgggagttt tgagactact atatgcgttt
     1321 ttttgttgat ttaccaacat gtttagacat tttcctctag gaaaaaatta actagcacca
     1381 cgggttttat tgggagcgtc aataattttg tgtaaataac tcgtccttct gcaaaataat
     1441 gggttactca gtaaacattg aagctaatgt atccgtcact tgttggaagc ctttatggct
     1501 tctatttaag aatttttgat tgtatgtatc aaagatggat actaggaaac gctctagtga
     1561 ttcttcattt tgaaactgct cttttctgcg gctgtatttc ttgatttgtt tattgaaaga
     1621 ttcgattaaa ttagttgagt aaatgcttcg gcggatacca ggcggaaact cataaaaagt
     1681 caataaatct tgatttttta tcagtgactg cgtcacttta ggataagttt tctgccactt
     1741 ctcaatcata ctccctaaaa aggtatttgc ctcttccttt gagctagctt gataaacagc
     1801 cttaaaatca tcacagattt cttttcgatc tttgacacgt actttatgag caatattacg
     1861 ggaaacgtga acacagcaat gttggtattt agctttagga taaatctggt gaatcgtatc
     1921 tttcatgcct tttaagccat cggtaataaa cagtaagaca tcatgaaccc ctctggagta
     1981 aatgtcctgt aacaactcat tccaaacgta ggtcgattca gtcggggcaa tcgcataact
     2041 tagtacttct ttagtgccgt cttctcgtat accaatggca atatagattg cttctttgga
     2101 gacggtttgc cgttttaaag gaatataagt agcatccatg aaaatagcga catacttatc
     2161 atttagagtt ctaactttaa atgcatttac ttcttcagtc agcactttag tcatgttgga
     2221 catggtttgg ggcgtgtagt gatgcccata cattttctca atcaagtctg cgatttccga
     2281 catggtaata ccttttcgga agaggtgaat gacggtctcc tctaaggtat cattcgtccg
     2341 tctataagca ggaacagtct gttgcttgaa ctcgccgttg cggtcgcgcg gaatctggag
     2401 atgaagttcc ccgtactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc
     2461 cgtgttaaaa ccaatgcgat cgtacttttc gtaatcgagg aaagccgtta actccgtttt
     2521 gagaagcgta ttgacagctt tttccaaatg gacacggaat aattcattca aatcgccttt
     2581 gttcgctaga gtctttagaa tttctgtagt aaaatcgttc atatggaagt cctcttttct
     2641 gtgaatgtgt tgtggtaact ctattctaca gaagggactt ccttttttct atttacacaa
     2701 aatattttac actctcgttt tattatgtta gaataaacag gtataaatag tgaaataaag
     2761 gaatgacaag caagagaagg aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg
     2821 aacaggcgct tatattgccg gcgcagtgat cttaatagca gctgcgggtg gcggttattt
     2881 ttactaccag cactatcaag aaacccaagc agtagaagct ggagaaaaga cagttgagca
     2941 atttgtccaa gctttgaaca agggagatta taacaaagct gcagaaatga catcaaaaaa
     3001 ggcggcaaat aaaagtgcat tatctgaaaa agagatctta gataaatacc agaatatata
     3061 cggtgctgct gatgtcaaag gacttcagat atcgaatcta aaagtagata aaaaagacga
     3121 ttctacttac agtttttcat ataaagcaaa gatgaatacc tcattaggtg aattgaaaga
     3181 tctttcttat aaaggaacat tagacagaaa tgatggtcaa accacgatca actggcaacc
     3241 caacttggtt tttccagaaa tggaaggaaa tgacaaagta agcctgacca cacaagaagc
     3301 agcaagaggg aatattatag atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa
     3361 acaattagga gtcgttccaa gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa
     3421 agcgattgct tcctccttcg acttaacaga agatgctatc aatcaggcga tttcacaaag
     3481 ctgggtacaa cccgattact ttgtcccatt gaaaatcatt gatggagcaa cgccagaact
     3541 tccagctgga gctaccattc aagaagtaga cggcagatat tatcctttgg gtgaagcagc
     3601 tgctcagctg attggttacg tgggagatat cacagcagaa gatattgata agaatccaga
     3661 attaagcagt aatggaaaaa tcggtcgctc aggtttggag atggcttttg ataaggatct
     3721 tcgtgggaca acaggtggga aattaagcat tacagatgca gacggtgtag agaaaaaagt
     3781 tctgatcgaa catgaagttc aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc
     3841 acaaaaaaca gcttttgaca gtctaggagg aaaagctgca tcaactgttg cgacaacgcc
     3901 aaaaaccggt gatcttcttg cgcttgctag ctctccaagc tatgatccaa acaaaatgac
     3961 aaacgggatc tcacaagaag attacaaagc ttatgaagaa aatcctgaac atccattcat
     4021 cagccgattt gcgacaggtt atgctcctgg atctacgttt aaaatgatta cagcagcaat
     4081 cggtctcgac aacggcacta tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg
     4141 gcaaaaagac agttcttggg gatcttttca aacaacgcgt gtcagtagtg atgtatcaca
     4201 agtagactta aaaactgctt tgatctattc cgataatata tatgcggcac aagaaacgtt
     4261 gaaaatgggt gagaaaaaat ttcgtacagg cttagataaa ttcatttttg gtgaagacct
     4321 tgatttgcca attagtatga atccagcaca aatttctaat gaagatagct ttaactcaga
     4381 tatcttgcta gctgatactg gatatggaca gggcgaactt ctaattactc ctatccagca
     4441 agcagcaatg tattctgttt ttaccaacaa tggcacactt gtctatccta aattgattgc
     4501 agataaagag acaaaagata agaagaatgt catcagcgaa acagcagtac aaacgatcgt
     4561 gccagatctg agagaagttg tgcaagatgt aaatggtaca gcacattctc tttctgcttt
     4621 agggattccg ttggcagcga aaactggtac agcggaaatc aaagaaaaac aggatgtaaa
     4681 ggggcaagag aacagtttct tgtttgcttt caatcttgat aaccaaggtt atatgatggt
     4741 cagcatgttg gaaaataaag aagatgatga ttcagcaacc aaacgagcat cggaactatt
     4801 gcaatacctc aaccaaaatt atcaataaaa aattgctttt taagtacagt aaaaggactg
     4861 tggcgttatc gtcacagtcc ttttttcaaa taaacggttt tcacgatgtc tctttcacaa
     4921 cttctttttg atttactcac acttcataaa ctttttttgt aaaatatttg agaacaagtg
     4981 gagaaataag tgtacttaat aaaatcacga taactaaagg tgaatagtac tgtggttcga
     5041 tcaattgact ttgctgtcca atttgtaaaa tgatcaaagc catttctcca cgcgaaatca
     5101 taccagcacc gaccatcaat gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat
     5161 aacctccaag caatttggtc aaaatcgcta caagcgtcaa tatagaaata aagagtagtt
     5221 gttcactaaa gtcagaaaaa tcaacttcta atcctacact gacaaaaaat actggtataa
     5281 atactgcata tcctaaagcc tctacattgt ggtatacttc ttctttaacc tttgtctgac
     5341 tgacagctat cccagcaaaa aaagcaccaa tgacagagct aagcccaaca agatccgcta
     5401 cgtaagacat tcctaaacaa ataactagcg atacgatgat aatagaagaa tttgcgtaca
     5461 ctttttctgc aaatgaaagt aggaaaggcg caatccattt gactaataag aaaataaaaa
     5521 caaaataaat tagctgttca atcaaaacaa gcggtaaaga agcattgcta gaagcttccc
     5581 ccgttagaaa agataggctg aaacttagaa ctaaaacgac taaaatgtca tctaccacag
     5641 aagcacccaa aattgttgat ccttcttttg tattcactac gtttagttct tttaatactt
     5701 ctactgaaat actgacagaa gttgccgcaa gaataatccc aaagaaaatc gcttcatttc
     5761 ccgaaacttg gaatgctttg cctgtcaacc agccaaaaac aaccgggaag atgatcccaa
     5821 gcaaagcaac gaacatacca ggtcggaaat attttttcaa aagagataaa tcactttcca
     5881 gaccagctaa aaacattaat aagatcacac caatctccga aaagtcatgg actagaatat
     5941 caggtttgac ccaattcaat ccagcttggc ctagcaaaac accaacaagt aattgtccaa
     6001 tcactgctgg gatacctaat ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata
     6061 tcaggcataa aataccaata aattccattc tcatcattcc tttgtataaa atatagcccg
     6121 aacttagcat aacatacatt attgaaaaaa taggaaaata tactcacaaa aaaaccaaca
     6181 atccaaaaga tttttttgga ttgttggttt ttatacggtc tattttaata actaaaaatg
     6241 attaacgtat agtcattttt cttattctga tgaaattgat aatctcctgg atgcccatga
     6301 aaatcaaaat aaagccgaat atttggaata gaagcatcaa acttttgaat ggattgaaca
     6361 ataagacgaa tccgccaatc aacaacagaa tactactata aacactccaa cttttagctt
     6421 ttgtattcat gctgaagaac aactggaaca agctgttgat gataacagct aatccaagaa
     6481 gaatcggcag gatagaaacg attgcaggtg caaaagcata gacgatcaga gctaagatca
     6541 ggaaaaaaag accactgaac agaccgatac tccaagaacc tgttttcctt ttaaaacggt
     6601 aagcctcaaa aagattcaac aacccaagca aaacaagata ggcggtgatc agataaccga
     6661 taaaatgaaa aacagcagaa gggtttagca caatcgcaat cccagaaata atataaacag
     6721 cagctcttaa taaggcgtat ttttgaatat tttgtttaat cgtctcaaac atataagaaa
     6781 acctcctttg acttatttta acgtgaacta gtcattttac caaattaaat gttacaaaat
     6841 gagcgaagga gaagaatatc tctggaaaaa tcaacttctg aggatctttt ccattttttt
     6901 gccttttgct agctcatcga tcattttatc taaataacga atttctttca tcaagttatc
     6961 ctcgatgtct tctactcgta caccacaaac aacacctgta atcaaggaac gtgaaggatt
     7021 ggggacaggg gcttccgcaa aaaattgttc gaaagtcact tgtttttgta gttgatattc
     7081 tagttctgtt tgggaataac ccgtcagcca acaaatgatt tcatctactt cctcttttat
     7141 acgatctttt ctctccgctt ttgctacata cataggataa acgcttgcaa aacttgtcgt
     7201 aaaaatacga ttttgcgcca taatcagtca cctttctttt tttcaaatta tttactactt
     7261 atagtatata agaaaaagag aagaaaaagc gattattcaa aaaaacaa
//
LOCUS       DARBEC010000114         7097 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.119, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000114 DARBEC010000000
VERSION     DARBEC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7097
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.119"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(1778..2443)
                     /locus_tag="SCL80_002526"
     CDS             complement(1778..2443)
                     /locus_tag="SCL80_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108364.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            2623..4605
                     /locus_tag="SCL80_002527"
     CDS             2623..4605
                     /locus_tag="SCL80_002527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEG1108365.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            4609..5247
                     /locus_tag="SCL80_002528"
     CDS             4609..5247
                     /locus_tag="SCL80_002528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108366.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            5275..5703
                     /locus_tag="SCL80_002529"
     CDS             5275..5703
                     /locus_tag="SCL80_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108367.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            6160..7071
                     /locus_tag="SCL80_002530"
     CDS             6160..7071
                     /locus_tag="SCL80_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEG1108368.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
BASE COUNT         2439 a         1066 c         1310 g         2282 t
ORIGIN      
        1 caccccgttg ttatatgacc gtgttttgag aggaaccaat aaaaagcata ggctaactag
       61 gggttccttt ttcttttgtt tttttggaat ccgcttaagt gaaatgtatc tgaaatgaaa
      121 cggagataaa gtgatttttt gctataatga caaggtttta gctgtttcag ataccatgta
      181 acccaaaata gtttattgag aaaataggag agtatttgat actaactagt aggaaaaaat
      241 gagataaaaa ttaaatggaa tcatttcagg taatctcaaa taaaaaaata tacagtcctg
      301 tggcacttta cctatgggac tgtatatttt ttactctcag cgggcttgtt ttgactccgt
      361 gtctgactcc gtcgtattga ttttaaatga tattcgatga ccatttaaca ggtcattttt
      421 gaaatttttg tctctatatg actcagtttg ataattattg atagatcaat ttgggtctat
      481 atcgaacaag ggaataatcc aataaaaaat gatttagaat atcaaaataa caaaaaagag
      541 gactacctgt attcgtcctc ttttttgacc agattttgac cagtctaatt tacattctaa
      601 tatattcata tgagctaatt ttttgattaa atggtaagtt tttccttgtt tgttttagtt
      661 taaactagtg tgtaatttaa aaatctatat ggaagggtct gtggggtaat acttaaataa
      721 aggctaggaa cctgataaca tcaaggttcc tagccttttt ataataaaat gaagcgaaaa
      781 taatttcttt taaagagccg agattatcag ctatataaga gtgaatttga tgcaaaaaac
      841 agagccgaaa cagtcttggt gttcgtagct ctgcttatat gtttcgaatt taagacaaag
      901 agttggcaaa agatgttgat aaggaagata agataactgt gttatataaa ctaggtaact
      961 aactgtaaca agctaatttt aagagattct tattagaaga cgtttaggaa tataactaag
     1021 aaaatgatta tacaggtaat tgtcatttca ataatgtttt ctaattttcg cgtctttgtt
     1081 ttaagaaaaa ggccagaatc agttggaaaa aagttcgtac gatagtgaag aggggcaaaa
     1141 tttcctacta atattcccac taacaagaga taaaacggcc aggttggtgt gattcctcta
     1201 taaaaaaatg cccatgctac cgaaatccca tagattaaca actgaacaat ttttacaatg
     1261 aaaaaattgt tctgccatta ctcaaagtct cttcctcctt gtatcgttta tttgtttcat
     1321 tttatcatta tgataggcgc ttgaacaatc cattttgtat tttattgagt actcttggat
     1381 aaactaccag cgattatcat atttatttat gttactgtaa gccatcatat aaaagtaaat
     1441 gagcagatca tagaaatata aaaaagaact aaaattgatc taaattatgt gcagcttgac
     1501 tacgtaccaa tctatattac ttactagatt ggtcgttaat ctattcggat cgaatgagca
     1561 aaatattttt gtgaatgtcc atccttgatt atgattccaa aaagaatcat tgggaggctg
     1621 tggcgtaaat attatactat aagccagaaa tacctatcat tctgaacttt tttatttagg
     1681 aactttcatc tcaaggcttt ttcaattaat ttgtacccct gttttttgga taagatcatg
     1741 tgcattgatt ttttctatct ctacgttcat ctcctactta atgcctatct ggctcctgag
     1801 ggcttctttt taaggcgtcg tcatagtctt ggctgaggat ttcttggtaa tctgagggaa
     1861 tggcttccca agtgagcagg cgaacgttca tgacgtgttt tcctttgtgt ttgattacag
     1921 catggttttt ctctttttct acctctccag cgtgaaaaga cagccatgta ccatggccaa
     1981 agatatcatt acatacccaa cgttcgatgg aattgtccac gtatcctaca gggagtgtgg
     2041 tatagccgat ttgtggtttc aaaaaaggat tgaatcgatc gatcgtttca tttttgtatt
     2101 gataattgat aagaaaaaga tagaccaaac aaaagacgag cagaaagaaa gatagtgtct
     2161 ttttgagtga gaagggttgt tttgatgaat aagatttctt gttaacaaga ggttcatctg
     2221 gcaatgttgg ttctattgat tcggtagctg attcaataga aggaaaatca atcggcagac
     2281 ctaaagtatg atgtaaaagt gacagcattt caaggtctgg cattgtacga gaattttccc
     2341 aactagaaat cgttttatcg gaaacaccaa ttttttcgcc taattctttt tgcgtaagct
     2401 tacaatttct gcggtaagtt cgtatttttt ctccggtgtt catcttcttc tcctttcgat
     2461 tctacagtat atagatttat tctacatgac ctatacactt gatagatacg tttttttgat
     2521 gaaaaacggt atgattttat caaagaatca tttaagtaag ggagagtggt gataatcaaa
     2581 aaaaagttca cagttttact cacaatcata gtgaccggta tcatgagtgg ttttgccttg
     2641 gttagtataa ataaacaggt actctatcgg acaaatgaat ttttccatca aacagatact
     2701 tatactccga ttcgattgcc acaacatttt attgggcaag aagaatatga gcagctaatc
     2761 atggtatttg aaaatgtctc atcagaaact gagctttctt atatgaaaaa aacagtaaat
     2821 gaatccaatg aagtagatac atcttggaac ttcaagatac agccaaagct tgaaatgaca
     2881 ttgtattatc aaggggatgt caaatcttct cacgagaagg taccctattc taatacctcc
     2941 tttcgcgtaa cgtccattga agaatcgatc aaaataaaag attttgaagg tgaatttttc
     3001 ttgatgacga gagaaaaaga agcctatgaa cggttcattt cactattcac tgatcgattc
     3061 aatcaagcat ttcacactga ttatgccact aatatttttg aagactttga aaatagctat
     3121 tcgtattcgg ttatttcaaa tacagacgta tataattatc gagtgtattt aactattgga
     3181 ttgattcttt tattcctttt gttatcatcc tttttcttat tgatgaataa agaaatcagt
     3241 ttattgttga ataatgactt ttcattacat caagtattgt ggtgtttatt ggggaaaaag
     3301 aaaatcatta ttacattcat aattgcagga atcattggtg gttttatttt tatgtattat
     3361 aaggagcaat ttctttattt gttccagatg tacgttttct tgttcagcgg aatatatggt
     3421 atctctttga tttctctttt attagttgaa aaaatcgctt cgttaagaga agcgttcaac
     3481 tatatccaat acattctttt tacatttgga ttgttgtttt ctcttctttt gaccgttgcc
     3541 aatgtcgaat tagcaactat cgttttttct tcttcacagt tagctgtttc ccatgaacca
     3601 ccaaaagaaa tcgctggtga ggactatcat gtattttatc cagtaaccat cgggaaaaac
     3661 cacgtggatt ttatttattc caatcgtttt gcatcagcag cagatcagga attatatgaa
     3721 actttgaata aaaacggaag tatattagtc aatgtatcag attacttgaa tgaagaaaat
     3781 gaacagtttg gcagagggat aaaaataaat ctgaattatc ttaagaaatt tcctttgatt
     3841 gatgtctctg gaaggttgat ccaaataacc gaaaaagaaa cgcaccctgt tatattgatc
     3901 cctgaacgat atagacatac agatttaaaa aatgacctgg cgcaaataac agaatattat
     3961 caagaaatca gtgaaaaaga accgaaattt ctatttataa aaaacaatca acccatttat
     4021 acatttattc cttctatccc ctggatcgaa cattatccgg ttgttgaaat actgacattg
     4081 aaaaacagta tgtatttgga aagaaatatt ctcagcggag aaatctatcc accgttaaag
     4141 atcaagatag gctctttgtc gaaagaaagg ttatttgagc tgattgaaga aagtcagtta
     4201 cgtgataact tgcagttatc atttccgtat actcagactg aagagatagc tgtcaaagtg
     4261 ttaagcagat catttcatta cttgatacag atatttttac tcactttctt ttctttcttc
     4321 ttgttgagct atgtcatgtt atgcatatat tttgtctaca attgtcggaa aatcgctatc
     4381 tttcgttcaa gtggttattc gctctttgaa acgtataaag atttcttcat gatgaacctc
     4441 atcaaatggg gaacgacaag tgtaatcttc ctttttctta tagaaagaga gcctaaatat
     4501 ctttttaata tttttttctt tagcttcatt gattttattc tttcagttgt ttttattttg
     4561 tctacagaaa agaaaagtca gttattgctg atgaatggag gttaacaatt ggagccgttg
     4621 atagtgataa aaggtgcgaa aaaaatcatt cgaaacaaaa cgattcttca tttggatcgt
     4681 ttcactgcgg atgctgggga tcttgtgact gttgttggag aaagtggagc aggtaaaagt
     4741 acgttattga atatattagg attgaatgat cagttcagtt caggacaatt ctatttgttc
     4801 gatcaagaga cgaaacaatt aactgggaga gagaagcgct tgataaaaag aaataccata
     4861 tcttttcttt ttcaagattt tgggttgatt gaatatgaaa caatcgattt taatttagat
     4921 attggattga agttttcgaa tttaaatgtt tcagataagc gaacagcaaa gtatgaggca
     4981 ttgaaaaaag taaatataaa caagaagcta agtacgccta tctacacatt atctggtggt
     5041 gagaagcaaa gaatcgcctg tgcgagagtc ttattgaaga atagcaaaat cattttagcc
     5101 gatgaaccga ctgggtcatt ggataaaaga aataaggata aagtagttga aatcttatcg
     5161 caacaagctt cccaaggtac gttagttatc gtagtcacgc atgatccata tctgaccaaa
     5221 ataggaaaca agaagatcat gctttaaaaa tcaaaaagga aaggatgtcg ttttatgaaa
     5281 aaagcagtta ttatttgtgc actagggata ggttttgttg gaggaaacgc tatgtcatct
     5341 gaagtggaag caaattcagt tcacggtgaa atctattaca aatctttaaa gccaggagag
     5401 attcattttg atgctaaagg taatattatt aagtcaaatt taccagtaga ccgggaaatt
     5461 tctagaacag caattaatca tagctctgga agatgggttt attatagtga tacaacttgt
     5521 acgagaaaaa caggtcattc taaccactat agtcgtgtct ataatcgtca cggtgcaaaa
     5581 gcgaaagttg gaaactctat tcgatcagca aatgcaacaa aaggagtatg ggcctatgca
     5641 agtgcgacag gaaatagaac tgacacattc tcatgttggt acaatccaac cggttggtat
     5701 taagtatcca aaagtttata taaaatagaa aaaacatata atgaaacagc gttaaaagtt
     5761 atttttggta gttttaatat attcattttt agttgtataa aaatatatca taataaaaac
     5821 aacaaaaaaa tgtctggaaa atcgaacata aaagtgtctg atttgaatat cccagcagcg
     5881 gaagaagaaa aacaaaatcg ataacatttg ttctaagaat tagttacgtt aaatacggaa
     5941 cgcaagatag acttgaaaaa cggtaacaaa agaagtagta tattaaggtg tgatgtgcta
     6001 cttgacattt attctgctga gcgaactttc tggattgtgt agccttatat ctattcagga
     6061 cgtatcgagc agattagtaa aggtgttctt gatgattttc atcttgaaca cctttgtttt
     6121 tagcaattcg caaaaaacga aaagtaggag aattgataaa tgaaaaaaag atttttagca
     6181 cttgccatcg tactaggcac tggcttattg tcaggctgta cgaatgccgg tgaaaaaaca
     6241 gccgttagtt ataaaggcgg tacaatcagc gaacaagaag taatggatag cttgaaaaaa
     6301 atgcaaggag cagattccgc tgtgcaacag ttgatcgttt atcaagtatt tgaagataaa
     6361 tatggagatg acgtatcaac caaagaaatc gattctcaat atgatcaaac aaagaaacaa
     6421 ttgggtgatt catttgattc acaattgaaa tctgcaggtt acacagaaca aacattcaaa
     6481 gacagtatca aacaaagtct agcctttcaa gaaggtctaa agaaacatat caaattgacc
     6541 gatgaagatt taaaaacggc ttgggaatct ttccatccag aagtggaagc tcaaattatc
     6601 caagtagcaa gtgaagatga tgcaaaagat gtgaagaaag ctgctgataa gggagacgat
     6661 ttctctaaat tagccaaaga caagtcaact gatactacga caaaagaaga tggcggaaaa
     6721 gtgaaatttg attccactac tacaacggtt ccggcagaag tcaaagaagc agcctttaag
     6781 ttgaaagatg gtcaagtttc tgatgtgatc acttctacca atgcttcaac ttatactacg
     6841 gaatattatg tggttaaaat ggtgaaaaac caaaacaaag gcaacgacat ggataaatac
     6901 aaaaaagaat tgaaagaaat tgcaacagac acaaaattaa gtgacagtac cttccagaat
     6961 aaagtaattg gcgaagtatt gaaagacgct aatgtaaaaa tcaaagacaa agactttgaa
     7021 aatgttttga gtacattcac ttcagattct agtacagcaa gtagtaaata agcggtataa
     7081 ataaaaaata tgaacta
//
LOCUS       DARBEC010000115         6782 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.120, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000115 DARBEC010000000
VERSION     DARBEC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6782
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.120"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCL80_002531"
     CDS             complement(57..1586)
                     /gene="ahpF"
                     /locus_tag="SCL80_002531"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEG1108369.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCL80_002532"
     CDS             complement(1596..2159)
                     /gene="ahpC"
                     /locus_tag="SCL80_002532"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEG1108370.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(2303..2965)
                     /locus_tag="SCL80_002533"
     CDS             complement(2303..2965)
                     /locus_tag="SCL80_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1108371.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            3149..4795
                     /locus_tag="SCL80_002534"
     CDS             3149..4795
                     /locus_tag="SCL80_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEG1108372.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAVLLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPNMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDLQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            4786..5868
                     /gene="uxuA"
                     /locus_tag="SCL80_002535"
     CDS             4786..5868
                     /gene="uxuA"
                     /locus_tag="SCL80_002535"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEG1108373.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFGLPGWEP
                     ERLAPIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLA
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNGYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(5976..>6782)
                     /locus_tag="SCL80_002536"
     CDS             complement(5976..>6782)
                     /locus_tag="SCL80_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M protein trans-acting positive regulator"
                     /protein_id="HEG1108374.1"
                     /translation="FIDLSVDILRPNLPICREQRILVNILARLSITKIESEETSLEQY
                     KLLKKANVPHVLYSREMVESLCTVFDLFIPEKEKILYSLAFARNKLYNGFLNFNQPNT
                     PLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILEKENVQWMVEDLVHLYDR
                     YKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQNDFMENCNIVISDCPLKQL
                     PSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQSKKENQVTS"
BASE COUNT         2105 a         1261 c         1277 g         2139 t
ORIGIN      
        1 aaaattcttg tcaccatcct tttttccgct ctctgacttt ttacaatctt catagctcaa
       61 gtgcgcatca gataatcgaa tgctcctaaa gctgcagtag ctcctgaacc cattgaaaca
      121 atgatttgtt tgtatggact atctgtacaa tcaccagctg cgaaaacgcc ggggatattc
      181 gttgcgccat ttttctcggt cactatttca tttcttggat tcatttcgat cgttccatct
      241 agccaatccg tattagggag taagccgact agaatgaata ctcctgaaac agctagtgta
      301 tgcatctcca tcgtacggcg atcgatatag gagagagatt ctacctgatt cgtaccgctg
      361 atttctttcg tcgctgcatg tttgataaca gtgacattcg caagagagac tagtttatcc
      421 tgtaagattt gatctgcttt taattctggt aagaattcta gaacatatac atgttttgct
      481 aacccagcca aatcgattgc tgcttcaaca ccagaattac caccaccgat aacagcaatc
      541 tcttttcctt taaataaagg gccgtcacag tgtgggcaat aagcaattcc tttattttta
      601 aactcttttt cgcctggtac attgatagaa cgccagtgcg cacctgtcgc gatgatggcg
      661 gtttttgctt taagtgatat accgttagct aagccaatat taataaaatg ttgctcttta
      721 tttaaggaaa cggcctgttg attcatcata atgtcgatag ggtaactacg cacatgactt
      781 tctacttgct gcatcaattt tggaccttct gtgtaagagg taccaatcat attttcaatc
      841 cctagtgttt caacgacttg tccgccgaaa gaatcagcga caagtcctgt acggattcct
      901 ttacgtgctg catagatagc tgcactacta gctgctggtc caccgccaat cactaataca
      961 tcataaggtt ctttttcaaa caactcttct tcaatcaaag ggccagtgat tttttcaaca
     1021 atttgttcga tcgtcatgcg tccgctacta aattcttctc catttaaaaa cacagttggc
     1081 actgccatga tttttttatc atcgatttct ttttggaaca tcccaccttc aatcatcgta
     1141 tgagtaatat ttggatttaa tacagctaat atattcaatg cctgtacaac gtcggggcaa
     1201 ttatgacaag tcaagctaac ataactgtca aaatgcaatg tttgtgttat ttttttgatt
     1261 ttaagactca aagattcact gattttcggt gcccgtccac taatttgtaa catagccagt
     1321 aaaaaagagg aaaattcatg accgaggggg ataccagcaa aaacgattcc actttctttg
     1381 ccaactggat tgatagaaaa actaggtgtg cgcatcgaat ctcttttttc ccaagagatt
     1441 ttaggagaca tttctgctat ttccttcgta aattcagcta gtttttgact attttcacta
     1501 ttatccaaac taacagaaaa aacaatagga gattcaatca gtgctaaata ttgttcgaga
     1561 ttttgtttag tttctttgtc taacatatcc accacctaaa tctttccaac aagatcaaaa
     1621 cttggtttca atgtttcgcc actttctttc cattttgccg gacaaacttc acctgggtgg
     1681 gtacgtacat attgagcagc gcgaatctta tcaatcagtg cactagcgtc acgaccgatt
     1741 ccatctgcat tgatttccat agcttgaacg attccgtctg gatcaataat aaatgttcct
     1801 cgctgagcta atccttgctc ttcatccaaa acatcaaaaa tacgagaaat ctgatgagac
     1861 ggatcaccga tcatcacgta ttcgatttta ccgatagcat ctgatgtatc atgccatgct
     1921 ttgtgagtaa agtgggtatc ggtagagcaa gaaaagactt caacacctaa agatttcaat
     1981 gtactgtatt gttcttgtaa gtcttctaat tcagtaggac aaacaaaagt aaaatcagct
     2041 ggatagaagc aaagaatact ccagttcccc ataagatctt tttcactgac ttctaaaaat
     2101 tctccttgat gataagcatg tgcattaaat tcttcgattt ttttcccaat caatgacata
     2161 gtaaaacctc caataataat tatttattta taattattgt aatttagttg gagttgcaaa
     2221 gtcaatggat attttgttat ttaataaaga ttactcaaaa acgaaggtta attataaaaa
     2281 tagaagaggt cgcaagaaaa tgctactgct ggaaatactc gggataacgt tgagtaacag
     2341 tttcctgttc tttcagcatc atgtgtaagt gcaatcccgt ttgaaatcga gcttcttctg
     2401 gttttttatc aactaaagcc ttgagtaaat cataatgttg ttccatgatc gtttcccatg
     2461 gaagcccctg aatggtcact cgtaaccagc ggaaacgttc taaatgggta ttatggtcat
     2521 ctaaccagtt ccaaacttct tttcttcctg caatttcgaa acaagcttca tgaaataaat
     2581 tatctgcacg gaaaaaagcg cgttcgtcat ttattttgac tgctttttct tgttcttcta
     2641 aaatcgtttc tagcactatt cgtgtttgtg tgtccaattt ggaacaacat tcaatcatga
     2701 tttgctcttc caactgttct cggacaaagc gagcattttc tgcagaaaca agattaattt
     2761 tggaaacata cgtaccactt tgaggtatcg tatacacgag ttcttgctga cgtagttgaa
     2821 tcaatgcttc gcgaatggga gtccggccga tagcaagatg ttcctccagt gccttttcag
     2881 atattttcct tccaggttct aagtcagcat agataatttg tttacgaatc gcttgataag
     2941 cttgtgattg caagctgctt ttcatcttta ttcctccaaa atcatttcta tgaacgtagt
     3001 ataacagaag aggtctgaaa aacaatctgg tatgtcacat taactagtaa atttgtataa
     3061 atagtaaaat tttggttgac atagcgattt ttattgcata aaattcactt gaatacaaaa
     3121 actaatatgt tagtttagga ggggaaaaat gattgattta aacaactgga ggaactttat
     3181 ccctgaatta tctcaaaaag aagtagaaat acctcatttt gaccgtgcat cattaagaga
     3241 aaatggacaa aaaaatcctg tctggatcca tttcggtggc ggaaatcttt atcgaggatt
     3301 tcatgcagaa atcgctcaac gtttagcgga tctaggcgaa ttgtccactg gtgtcattgt
     3361 ttgtgaaaca ttcgatgaag aaataattga aagggcttac caagattttg atgatttgat
     3421 tcttgaagtg gtcatgccaa gcagtggaga gttcgtcaaa cgtatattag ctgcgacagc
     3481 tgacagtctg tattgtcatc cagattgttc tgaacacttt gataagatga ttcgacactt
     3541 tgaaaatcct agtttacaat tagttacttt tgctattact gaaaaaggtt atcgattgaa
     3601 agatacaaat aataaatata taggaccgat tgcagtcgat ataaaaaatg gtccaaagaa
     3661 gccatcacat acaatgagta ttcttgctgt gctactttat aaacgttttt tatctggagc
     3721 ttggccgatt gcacttgtct ctacagataa tttttcacaa aacggtagtc aattacgtac
     3781 ttctttgcta acgatcgcaa aagaatggca aaaggaaggt tatgtacaaa atacatttat
     3841 ccaatactta tcgaatgaag aaattgttgc gtttccatgg acgatgattg atcgtatcac
     3901 cccgaatcct tccttgtcag ttagcaaaat gttggcaaaa gagggctttt caaatatgtc
     3961 gattttccaa accgcaaaag gaaataatat tgctccattt gtcaatacag aggacatcca
     4021 ttatttaatc atggaagatc attttcccaa tggacgtccg aaattcgaaa aagctggggt
     4081 attcttgact gatcgtgcaa cggtagataa agcagatacg atgaaggtga cgacttgcct
     4141 taatcctttg catacgtctt tagctattat gggttgttta ctggggtata ggagcatagc
     4201 tgaggagatg aaagatccag atttatcgct gctagtttct gaaatcgggt acaatgaagg
     4261 cctcccagtt gtggagtctc caggtatcat tgatccaaag atctttctgg aagaagtaat
     4321 tactgtgcgc ttttctaatc caaatattcc taatatgcct cagcgaatcg ccacggatac
     4381 ctctcaaaag gtggcgattc gttatggtga gacgattaaa aaatatatgg aaatagaagt
     4441 taaagacccg cagacacttg tattcatacc attgactctc gctgcttggc ttcgatacct
     4501 tattggcatt gatgatcaag gaaaaccatt catcccgagt ccagacccac tattgacaga
     4561 ccttcagcat aagctttccg atattcaact gggtacgcaa gatacatcac atattcatga
     4621 gaaaatggct cctattttgt caaataaaga aatttttggt gtagatcttt accaagcaga
     4681 gcttggcaga aaaatagaag aaatgttcat caagatggta gcaggaccaa atgccgtacg
     4741 aaaaacatta caggattact tacaagaaca tggaggaaat ttatcatgga aatgactttt
     4801 agatggtacg gatcacagga agaaaagatt accttagatg aaatcagaca gattcctgcg
     4861 gtcaaaggga ttgtaggcac attgatggat ataccagtag gagaggtatg gcctagggag
     4921 aggatcaaag aactaaaaga agaaattgag tctcgtgggt taacactaaa agtaattgaa
     4981 agcgtcaatg tgcatgatgc cattaaaatt ggattacctt cccgtgagca gtatatcgaa
     5041 aactataagc agacaatccg aaacttggca gaatatggaa tagaagtcat ctgctataat
     5101 tttatgcctg tatttgactg ggtgaaatct gaccttgact accgcttaga agacggctca
     5161 tccacgttag cttttatatc tgatgatatt ccagatgatc caagagaaat cgtcgcgcgc
     5221 atcgaacatt ccagcaatga atttggattg ccgggatggg aacctgagcg cttagctcct
     5281 atcaaatcct tgcttgaagc ctatgcatca gtagacgaag aaaaacttcg agaaaatttt
     5341 gcctattttc tccaatcaat catcccaaca tgtgaagagg taggggtcaa aatggctgta
     5401 catccagatg atccgccata ctcgattttt ggattagctc gaatcgtaaa gaatcgagaa
     5461 gatttagatt ggttgtgtcg catagtagac agcccttata acggaatcac tctttgtaca
     5521 gggagtattg cagaagatcc agaaaatgat gtatatggga ttttagcaga attttgccaa
     5581 agagatcgaa tcccatttgc acatgttcga aatattaaat ttttagctaa tcgagatttc
     5641 tatgaggcac cacataaatc cgaatacggt tcattagaca tgtaccgcat actaaaaaca
     5701 atgtatgaca acggatttaa cggatacatc cgtcctgatc atggaagaat gatctggaat
     5761 gagacaggaa gaccaggata cggcttatat gatcgagcat taggtgctgt ttatctaaat
     5821 ggattatggg aagcaatcga aaaaggaaac aaaagtaaag ataattagtg aaaaaaactg
     5881 taattttatt atgtaaataa gaaggatgtt caaattcttg atacataaat ccggaggttg
     5941 ttaataagca ttcggattta tgtatcttat ttggtctagg aagtaacttg attttctttt
     6001 ttcgattgat ccatgagtgc ttcttctttt aattcaaaga tatattctga gatcctcata
     6061 atcacttttt tccagtcttc tggtgtgatg attcctttta aaatatagat ttttttgata
     6121 tgagaaggca attgtttcaa agggcagtcg ctgataacga tgttgcagtt ctccataaaa
     6181 tcattttgta ttttgatcga ttcatttgaa aaaatttgtt ctatcttaac gatcagatct
     6241 ttactgatat aaaaatcacg cgtatagttc acaccaatca caattcgcgg ctgttttttg
     6301 tagcgatcat acaaatgtac taggtcttca accatccatt gcacattttc cttttctaaa
     6361 ataactggta acaagtgttg attttgttct ctgaaatcca tataaaattg ttcaatggcg
     6421 gtttggactt ctttcaatac gctatgttct tgataaagca tgaaagtagg aaggggcgta
     6481 tttggttgat tgaagttaag aaatccatta taaagtttat ttcgtgcgaa tgccaatgag
     6541 taaagaatct tttctttttc aggtataaac aaatcaaata cagtacaaag ggactccacc
     6601 atttctcttg aataaagaac atgaggaacg tttgcttttt ttaaaagttt gtattgctcc
     6661 aagcttgttt cttccgattc gattttcgtg atagaaagtc gagctaaaat attcactaag
     6721 atgcgttgtt cgcgacaaat tggcagatta ggtcgcaaaa tatctacaga gagatcaata
     6781 aa
//
LOCUS       DARBEC010000116         6570 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.121, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000116 DARBEC010000000
VERSION     DARBEC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6570
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.121"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            127..579
                     /locus_tag="SCL80_002537"
     CDS             127..579
                     /locus_tag="SCL80_002537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108375.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            593..1282
                     /locus_tag="SCL80_002538"
     CDS             593..1282
                     /locus_tag="SCL80_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108376.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            1407..3758
                     /locus_tag="SCL80_002539"
     CDS             1407..3758
                     /locus_tag="SCL80_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEG1108377.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            3838..4233
                     /locus_tag="SCL80_002540"
     CDS             3838..4233
                     /locus_tag="SCL80_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108378.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            4558..5175
                     /locus_tag="SCL80_002541"
     CDS             4558..5175
                     /locus_tag="SCL80_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108379.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            5216..5515
                     /locus_tag="SCL80_002542"
     CDS             5216..5515
                     /locus_tag="SCL80_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEG1108380.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            5549..5716
                     /locus_tag="SCL80_002543"
     CDS             5549..5716
                     /locus_tag="SCL80_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1108381.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(5761..6366)
                     /locus_tag="SCL80_002544"
     CDS             complement(5761..6366)
                     /locus_tag="SCL80_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108382.1"
                     /translation="MGKIGYIRVSSQDQNLDRQLAMMEGLNIDKLFQEKASGKDTERP
                     EFQKLLNYIREGDCVIVTSLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQ
                     KRLVFKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2265 a          910 c         1285 g         2110 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc aattatctta tcgtagatat atattataaa tttaataaga aagcgaggtg
      121 agaatcgtga aaaaaacgat actaagtatt ttttcagtat ttacaatatt aattgggggc
      181 tattttcttt tgaatttaga ttctgttaat tcaaacgctc aaataaaaga tcaaagagta
      241 gctgaacata ataataaaaa aatgagtttg accaaaaata tctattctat gtcagatgaa
      301 gaattgtata aagaatttgg tgatttatcc ggggaacagt tagaagaaag tattccagga
      361 attagtgact cagataaaat tgttgtcttg tctgctggtg ggcaattaat gggagatggc
      421 aaatccgaat tagttagtta taaacaaaat cacagacagt ttattgatgt taatactgat
      481 tccaacgcta tttcgattaa ggatctaaaa gagcttttaa atagtaaaag aaatgaatta
      541 aagcagataa aagaaactaa ttcagtggaa ttagagtaaa ggaagattca ttatgaaaaa
      601 gatttttggt gtttctgttg ggctacttat gatgttaaca tttggaaatg cagtatatgc
      661 tgctgagaaa gaagttattt cagatccaga gcagtacgcc tcacgatact ttgctgattt
      721 tgaagaagaa gataaagtac ttgcattgcc agaaggtggt tttttaaaag gggaagctaa
      781 aattgtatct atggataatc cagatataat tcaaggttca tataattcag aaactgatcc
      841 tgcttcagtt tcagtgacag aagcaaaaaa aattgttgct gaaaaattca aaaatgatat
      901 tgaaactact ccaattatgc ctcgaggagc atttccaccc gatgttggta ctagcggaca
      961 ccttcttgta ctagcaggag gaggaagcta taaatcacag ccatttagtg gtgcgggatg
     1021 gcgttttgga ggtatgggtg taaaaccagc tgatggtaca ggcaactttt tacgttggaa
     1081 atcctacatt gacgatggaa gagtaggaaa tgtaaatcaa gccaacgcaa ctaaatctgg
     1141 ttctattcaa ggaatggcat tgtatgctcg tgaaggattc aaatactttg caccaaacgg
     1201 gcctggttgg acaatgaata taggtattta ttacacttat aatcctgttg caggtacgta
     1261 ttatgttgtt caaaatattt aataaaacag cacaaagaga tttctttgtg ctgttttatt
     1321 atttttagtt gttgacaaac actaaaatat aaaatataat caacacataa acgaacgtat
     1381 gttcggcaag aaagcagggg atttttatgg atgagatggt tttaagtact cagaaatggc
     1441 taaacaagaa atacagtaat gttacagggt ttgataaggt tccagaaaat gggcgaacag
     1501 ggtggcctac aatctatgga cttattgaag gactacaagt agaacttggt attactaatc
     1561 tagtagctaa ttttggacct accactgaaa aaatgtacga taatcaggtt actcctaaat
     1621 ggggcaaaaa tctacctaaa aatattgtat ttcttattca aggtgccttt tggtgtaagg
     1681 ggattaatcc aggtggcttc gatggggtat atactccata tttagatact gctgtaaaag
     1741 agttgcagac tgatgcaggg tttaatggaa gcgctgtaac tggagttttg gatgcgaaat
     1801 gggccaaagc gttatttgat atgagtgctt ttgttcttgt tcccggcgga gatagtaaaa
     1861 ttagaaaaat gcagcaatgg ttaaatgtaa attatttaga atacacggga ataatgcctt
     1921 gtgatggtat ttatcaaaga aattcaaatc aagcattaat ttttgcatta caagctgaac
     1981 taggatattc tcccagtgaa gcaacagggt cttatggtaa tgggacaact agtaaaactt
     2041 atcctgtgtc tgaaggtaac tcgggaaact atgtaagaat aattcaatat ggattatatg
     2101 ttaatggtta ttttgaagac ggtacattcg atggtatttt cactaaatat atgggtttag
     2161 aagtactggc attccgaaaa tttatggtat tgcctgaata tactgaaata gcagatgttg
     2221 ttgtaataaa aggactattg tctagcgctg gagatattag aagatctgca gatggcgcag
     2281 atacttctac gcaattaact agatctcaaa tccaaacatt ggttgataat gatataaaaa
     2341 tagttggtag atatcttact ggtactgttg gaattggaaa agatgaacga aataagtatc
     2401 taactacaga ggaattgaat aatatttttt ctaaaaattt atctgtgttt cctatctatc
     2461 aagatggagg tgcagcatta gcttatttta cttatgatcg tggcttatca gatggtaaga
     2521 aggctataga tgcagcaaaa aatctaaata taccattaac aacagttatt tattttgctg
     2581 ttgatctaga catgttaggg gaagaaattc ttgcatatgc tggagaatat tttaaggggg
     2641 tatctgctgt aatgtcatat agcgggtatc aaacgggtgt ttacggaaca agaaatgttt
     2701 gttctcaaat aatcaataac gggtatgctt cgtttgcatt tgtttcagat atgtcgactg
     2761 gttatagtgg gaatttaggt tttcctatgc ctagacaatg gtcttttgat caatttgttg
     2821 aatttaccat aggaagtggc aacggagctg ttggtatcga tttaatcgct acatcgggta
     2881 gagatagcgg atttaatgag ttatcaaatg atactaataa tgataattac attgccaaat
     2941 acaatcagaa ggttataaat cagatggtac gtgcgtatca atatctatca caagctggtc
     3001 ttgataatcc ttggaaccct catttgacat tttatagata tgtaaattat tctggtttgt
     3061 catgggacat catttcttct cctgtaacag agcatgatag aaaaatttat gatgaatata
     3121 ggaataaatt aactaatagc gagggcctgt ataattattt tatagatcct aatactggtt
     3181 caataatagg tttgcctcac ttaattgtga cgttacaatc acagatgttt ctagccgaca
     3241 caatcaatga tagtgtttct gactttgcag gttggttagg agatttaatg acttgctggg
     3301 gtgaagttaa gaaattagga atacaaatgg agcaaggtgt atttgcatta gtaggtacgg
     3361 ctgcaggtgg aacatttagt ctagaagatt tataccaaga tgttgacgcg gtaaatgttt
     3421 ataccttagc aaaaagaaat tctgattcaa cagctttatc ggctttaaaa acgtactatt
     3481 gtgataaggg tgctattgat agatttaatc aatttgttaa gaacagattt ggttcttcag
     3541 ataaaatcgc ttcagtaaca aatagtttat tagaaaatga cgaaacagtt gatatcttag
     3601 gtgtagggat aatttcattt ttcattgata aaatagttaa taattcactt gggacagatt
     3661 ttacttatct tgattttatt ggagatgagg ggaaagttgc aaatggattt tctgaaaaaa
     3721 ttattcaatt tcaagaaaat agaagtaaac ttaattaaga atcgtttact atttaaacag
     3781 tgtccttttc ctttttaaat atatacgata aaataagtat atgaattgta aaaggacatg
     3841 atgattatgg aagaaataga atggaataat ctgactcaaa ataataaaaa taattctgga
     3901 tgtggaatta tctttatgtt tgttatagtt ttgggaatag tagtactttt aataactgtc
     3961 aagatagtag attactttgc gaatactcct gaagaacaaa tacagagaaa acttcctgag
     4021 tttgaaagag taccacctta tgatccaaga gataattcat ttcaagtaaa aaaattaaaa
     4081 gagtctccaa atgaaatgtg gtttgaagtt acagttgaat caagcgactt caaagaaaaa
     4141 aagaaagaat acaattatat tgtaaaggca aaagtatcaa aaagatttat ttttaaccct
     4201 aaattagaaa tatatagtgt tgtaatagaa taatagtgtt aaaaaaattg taaaagttaa
     4261 agaagtcaaa ttctgatgtg aaaaataaaa tataccatgg aagacataaa agaaaaactg
     4321 gataactagt ttatttaatg gcagaccgtt aaggcctgcc atttttatgt attttagcgg
     4381 aataaatata atcatgcggg agtgtaaact tactgtttca aaaagtatac tttttgacat
     4441 gccttgattt tctttgttta tgtatggcat aataaccaca taatacaatt tgaaacgttt
     4501 ctgatttaat ctgattgttt tgaaacaatt ttagtgaaaa aagggtagat aaaaacgatg
     4561 agaaaatttt tttatatccg ggtcagtagt tatgatcaat cgatcgaacg acaactgatt
     4621 tccgcgaagg aattaggaat ccctgaagaa tatatatttg tagaaaaagc cagcggcaaa
     4681 gattttaaac gccctgaata tcaattaatg aaacggatgt ttcgaaaagg agatttactc
     4741 tatctacaat cattagatag actaggcaga aataaacaaa tgattttaga agaatggcga
     4801 gaattaacta aggaaaagca gattgatatt tctgtgttag acatgcctct gttagatact
     4861 gctagttaca agcaactaga tgggttagag acgcttatct ctgacttaat tctgcaatta
     4921 ctcagctata tggccgaaga cgagcggaaa cgaattaata gccggcaaag agaaggcgta
     4981 gaagctgcga aacaaaaggg tgtaaaattc ggcagaaaga aaattgcttt acctcctgaa
     5041 tttcctgcga tctattctga ctggaaagca ggaaagataa ctgcagtcta tgctatggat
     5101 caattgggca tgaaacgaaa taccttttat cgacgggtga aagaatatga acataccttg
     5161 caccactttt cttgatagat ataacagcta ttagacggga agggagagtt tgaaaatgga
     5221 cttaacgaat caaagggtcg tttttactgg aacgctgcag caaatgacac gaacccaagc
     5281 catcggtttg gttcactctt taaacggcca ttgtcaatca agtgttactt caaaaacaaa
     5341 tttcctggtt tgtggtcagt attctaagtc gttgtttgat gattctctct atacaaaaaa
     5401 ggaacaaacc gcgcgggact tgattgcgtt aggtcatgag attaccattt tatcagaagt
     5461 cgaattttat gcgttaatta gtcaacagtt gaaagaatgg caacggtatt tataatcgtt
     5521 agtaaaattt acaaggagga aaatttttat gaagcgaaaa tcttatgatc gttggggcat
     5581 tgaaataact agttttaatc gaaatattgc ttttcacgtg gagtgtgact gtggggaatt
     5641 agcaaaaagt gtttatcaaa aaaattactt ttactgtcct aaatgccatc gccgatacgg
     5701 ggtacatgga acatagaaac gaaatagtgc ggtagaaaag cgaatgaatg tagtatattt
     5761 ctacaaatca ttcgctttct ttattttata aatagttggt cgagagatcc cgttttcttt
     5821 tgcaatctct gcaactggaa tgccttgctt taacatgtcc accacggttt taaaaactaa
     5881 gcgtttttgc ggatctgttg agttaataga atactcgatt ggtcgtccct tatatcggcc
     5941 ggctttttga gccaataata ctccttgccg ctgacgttca cgaattttct cacgctcgtt
     6001 ttgtgcaata taacttagca gtgataaaaa catatcgaac atagcggtat ccaggagttc
     6061 atttccagta ttaaaatcta aaaactgtgc atctaatatt tttaacgcta cttttttctg
     6121 tttcataaaa gcaactgtat tttttatatc ttcataatct cttcctaaac gatctagtga
     6181 tgtaacgatg acgcaatcac cttcacggat ataatttagt aatttttgaa actcaggacg
     6241 ctcagtatct ttaccactgg ctttttcttg gaataattta tcaatgttaa gtccttccat
     6301 cattgccaat tgtcgatcca aattttgatc ttgtgaagat actcgaatat agccaatttt
     6361 tcccataaaa aactcctttt taaaagtgat ataatttaca tgtaaaattt acttgtagtt
     6421 ttactgctat catagcattt ttaaaaataa attttatgta gacttaaaat ttacttcatg
     6481 gagggtttaa atggatgaaa tcaaaagtaa caaaaaagca tttggcattt atttaggaga
     6541 acctagtaca gaacaattaa accaattttt
//
LOCUS       DARBEC010000117         6287 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.123, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000117 DARBEC010000000
VERSION     DARBEC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.123"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..177
                     /locus_tag="SCL80_002545"
     CDS             <1..177
                     /locus_tag="SCL80_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108383.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="SCL80_002546"
     CDS             190..690
                     /locus_tag="SCL80_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1108384.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="SCL80_002547"
     CDS             787..2634
                     /locus_tag="SCL80_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEG1108385.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="SCL80_002548"
     CDS             2637..3533
                     /locus_tag="SCL80_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1108386.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="SCL80_002549"
     CDS             3583..3972
                     /locus_tag="SCL80_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108387.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>6287
                     /locus_tag="SCL80_002550"
     CDS             3959..>6287
                     /locus_tag="SCL80_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1108388.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGGQAVIVDPKGERGGWKE
                     TLPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILTFLTGISSRDGEKF
                     PVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTDYDFAHLLFSDGSI
                     TQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVISTFALDFIHSNREI
                     FKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDDLLDERLKNNIGLK
                     FAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT         2118 a          933 c         1348 g         1888 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccgg
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tggtcaagct gtgattgtcg atccaaaagg
     5461 ggagcgagga gggtggaaag aaaccttgcc agaaattgcg gatgaaatca gtatcctcaa
     5521 cttgacgagt aagtcagaaa atcaaggtct gcttgatcct tatgtgatta tgaagaaaaa
     5581 gaaagactcg gaaagtttag caattgatat tctgactttt ttaacaggaa tttcaagtcg
     5641 agacggggag aagtttcctg tgttacgtaa agccattcga acggtgactc aaagtgatca
     5701 acgggggtta ttacaagtta ttaacgaatt gcgaaaagac gggtcaccaa tcgcggaaaa
     5761 tatcgccgat catatcgagt cctttaccga ttatgatttt gctcatttgc ttttctcaga
     5821 cggctcgatt acccaatcga ttagtttaga taaacagcta aatatcattc aagtggcgga
     5881 tttggtttta ccggatgcag aaacgacttt tgaagaatat accaccatgg aattgcttag
     5941 tgtttcgatg ctaatcgtca tttccacttt tgccttggac tttatccata gtaaccgaga
     6001 aatcttcaaa attgtcgatt tggatgaggc ttggagtttt cttcaagtgg ctcaaggaaa
     6061 aacgttatcg aataagcttg ttcgtgctgg tcgggccatg aatgctgggg tctatttcgt
     6121 cactcaaaat agcgacgatt tattagatga acgtttaaaa aataatatcg ggttgaagtt
     6181 tgcttttcgc agtaccgata ttcatgaaat caagaaaacc ttggaattct ttggtttgga
     6241 tcaggaggat caaagcaatc aaaaacgcct gcgagatttg gaaaacg
//
LOCUS       DARBEC010000118         6120 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.124, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000118 DARBEC010000000
VERSION     DARBEC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6120)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6120)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6120
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.124"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..168
                     /locus_tag="SCL80_002551"
     CDS             <1..168
                     /locus_tag="SCL80_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1108389.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVAPDDAQRLSENLKIMQ
                     KELQKIWQQLK"
     gene            150..1064
                     /locus_tag="SCL80_002552"
     CDS             150..1064
                     /locus_tag="SCL80_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1108390.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     TLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPNHQIAIYTHTDKDHIHN
                     HIVINSVNLETGKKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKTEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYTQFCDSLKTFGIHCLNRGKNFTYELVHKKK
                     KVRSNKLGKDYEKETILHELDRRERTYNQQRTNELRRARASIRETAEEVRREQRTFTP
                     EQSEIRPTRRNHEQHVERGTDHQQELSL"
     gene            1309..1998
                     /locus_tag="SCL80_002553"
     CDS             1309..1998
                     /locus_tag="SCL80_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108391.1"
                     /translation="MAFYLPYLLISASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLV
                     GVIFALSIVLSGLFGVLEGNKRVSRSILLGDITQKYEAACDKKKKEQALAQKMEEFTA
                     CYEDMNNIFVDQEKRLTDKNMKKLTRFYQNLPEKQQKEVQEKYEQVKKDVQYFKDTQT
                     EEACCNLFSDTIPFSTSEQERKERQQSVTYERYETLLQQATNIQNPTKKETALNYLES
                     VKEWLDQQQQN"
     gene            complement(2119..2205)
                     /locus_tag="SCL80_002554"
     CDS             complement(2119..2205)
                     /locus_tag="SCL80_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1108392.1"
                     /translation="MFERFICPLLVEVCVSLFSYWLNNRNKK"
     gene            2881..3831
                     /locus_tag="SCL80_002555"
     CDS             2881..3831
                     /locus_tag="SCL80_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1108393.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     LTFDQLKDLSNYKATANNRFINDLEMTYDKLMDLRFGKRSANGLDIDRFVMFSEFKIR
                     GTAKDPYVDIQVYEKALPLLNNLDEWVRYSLQQFNELQSSYAKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYWNNQANVDNRVIKPIRQELTAIFKGLSIKKKYGKGRGKPVV
                     GYQFTFKPEIKKADDFNKHAPTKEEQNKALALADKQKEYEEPATTINEKIKQKKRELE
                     LLQQIKQLEKEKEALEKQTESALSKKEKKSLLSDLDNLFQ"
     gene            3852..4385
                     /locus_tag="SCL80_002556"
     CDS             3852..4385
                     /locus_tag="SCL80_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1108394.1"
                     /translation="MQPQETFTIKELSELFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLGNQMLLDRLHEYEQDNDKNVNIPSKNVVTNKLLDQLREENEFLRE
                     QLALREQNNSHIQKLLDQQQQLTLQANKQIEKLQEKLQLTYTEEAPENDSTTLSEATE
                     TIDKQIDTEKKWWQLWK"
     gene            4401..4571
                     /locus_tag="SCL80_002557"
     CDS             4401..4571
                     /locus_tag="SCL80_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108395.1"
                     /translation="MTRKNLLFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
     gene            5021..5554
                     /locus_tag="SCL80_002558"
     CDS             5021..5554
                     /locus_tag="SCL80_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108396.1"
                     /translation="MKKFTLGVALLTIGAVVLPSTTIVYASENSQVVSLNENNATENV
                     VIEYTEDGMVVTKYEDGYSAEDFANSSDIMTRAAVARWGKKEYTNIAVTTGVAANAIN
                     AAFWAGGKASTSMFGIPGWAIGALLTGASWTKKGASPGNAVAKEWDKNGNGWIGFYYQ
                     RGYDAAGRVVATRYSTL"
     gene            5566..5769
                     /locus_tag="SCL80_002559"
     CDS             5566..5769
                     /locus_tag="SCL80_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108397.1"
                     /translation="MFLISKSLKSLKTFTKKNPQIATIRIFIFAIAVAIIVNMLSGGQ
                     LYKSGLFLVLILVVLDIIKSKKK"
BASE COUNT         2266 a         1059 c         1129 g         1666 t
ORIGIN      
        1 caggcaatcg cgcgcgattt agccaaagct ggagggaata tcaatcaaat tgccaagtgg
       61 tgcaatacac accaacacga cgttgctcct gatgatgctc aacgtttatc cgaaaaccta
      121 aaaatcatgc aaaaggagct acaaaagata tggcaacagt taaagtaagt cgaaccactt
      181 catgtagccg agcaatcaat tacgcagaac cacgagcaac cgtcaaaaca ggaataaact
      241 gtgacattga ttatgccaaa agtgaaatga aacaaatacg cacgctttat ggaaaagacg
      301 atcgtgtaca agcacactta ttgattcagt cctttcgacc aggagaaatt accgctgaaa
      361 aagccaatca actaggggca gagtatgcga aaaaaatagc ccctaaccac caaatcgcga
      421 tttacacaca tacagacaaa gaccatattc ataatcacat tgtcattaac tccgttaatc
      481 ttgaaactgg gaaaaaattt caagcccatg ggcaagcatt tttaaacaaa tgttatgacc
      541 taaatgacga gatttgtctt gaacatgact taacaatcac ggaacgagga aagaaaaccg
      601 aaaaacgaac catgtctgaa attaaattaa aagaaaaaaa tgaaccggtt tggaaagacg
      661 aaattcggtt tgcgattgat caaacaatga aaaacaaaaa aactcgaacg tatacccaat
      721 tttgtgattc acttaaaaca ttcggcattc attgcttgaa tcgcggaaaa aattttactt
      781 acgaattggt ccacaaaaag aaaaaagtac gatccaacaa actaggaaag gattacgaaa
      841 aggagacgat tttacatgag ctggacagac gagaacgaac ctacaatcaa caacgaacca
      901 acgagctacg aagagctcga gctagcattc gcgaaactgc agaagaagta cgaagagaac
      961 aacgaacttt tacgcctgaa caatcggaaa ttagaccgac tcgaagaaac catgaacaac
     1021 acgttgaacg aggaacagac caccaacaag aattatcgct ctaaaacgtt aaaagacatc
     1081 gaaaacgaaa taaagagaca acgtgccttt cttgcgcctt atacccaaga aaggatacaa
     1141 actacgatgg attcacaagt caaaaaactc caccaagcgc tattagacca gcgaaaccaa
     1201 gaacaaaaaa aggctttaat tcaacgatcg atggatattc ttgccccctt atgttgctta
     1261 gtactaacat tattagcctt ttggttggta tggggagaat aaacagacat ggcattctat
     1321 ttaccctatc tactcatttc tgccagtatt ttaggaatta tttggcttag ctataaggct
     1381 tatcaaacaa gatattcatt aaaaggctct aaaattcgat ttaagcgatt tttattggtc
     1441 ggggttatat ttgccttatc aatcgtttta agtggtttat tcggcgtttt agagggtaat
     1501 aaacgtgttt caagaagcat tttattaggg gatatcactc aaaagtatga agcagcatgt
     1561 gataaaaaga aaaaagagca agcattagca caaaaaatgg aggaatttac cgcttgttat
     1621 gaggacatga acaatatttt tgtggatcaa gagaaacgtt taacagataa aaacatgaaa
     1681 aaactcactc gtttttatca aaacttacca gaaaaacagc aaaaagaggt acaagaaaaa
     1741 tatgagcaag taaaaaaaga cgtgcaatat tttaaagata cgcagacaga agaagcttgt
     1801 tgtaacttat ttagcgatac aatccctttt tcaaccagtg aacaagaacg aaaagaacgg
     1861 caacaatcgg tcacatacga acgttatgaa acactgcttc agcaagcaac aaacatacaa
     1921 aatccaacga aaaaagaaac ggcattaaat tatctagaaa gtgtcaagga atggttggat
     1981 cagcaacaac aaaattgaat ttgattagca atcatgttaa actgaacctg caaatgatca
     2041 aacattagat acacgaaagc cccagagagg tcggattctc tggggctttt tttcaggttg
     2101 atgtcgcctt tattgatttt acttcttgtt tcggttattc aaccaataac tgaataaaga
     2161 gacacaaact tctacaagaa gggggcaaat gaaccgttca aacattagat acacctcctt
     2221 ccagtcgcaa gactgtcgga taggcgacaa gttattatac cttacaaaat aaaatctgtg
     2281 tggtgttttt aagtatccct tattttcggg ttgttagggc ttgccctgac cgcctgtaag
     2341 gcgctttaag aacaagataa ggttttttac ccaagactgt cttaaaacga cttagacgag
     2401 ctatcagagg cttcccccac ctccgttact gacttgtttt tagcaaagga gcctttaggc
     2461 gactgcgcag tacaatgtga gagcatagcg atcacacttt tttagcgacc gtagggagca
     2521 aggttttgaa ttggcagttt tgaaaaatgc gcgaacctgt tggtagaaaa aagaagtggt
     2581 ttttaggcgg gaaattttgt caccctcttt tataatctct ttttaaacct ttttttaaac
     2641 ctctttaccg tcggctgtgt ccttactcct tcaatggata cagcgtttta taaactacat
     2701 tttgtctgct tttaaactac attttgtctg cttttaaact acattttgtc tgcttttaaa
     2761 ctacattttg tctgctttta aactcttttt ttgcactcac gtatgttata atatgcatta
     2821 tcacagacaa aacgttgttt ataacgtctg tatccattga aggagtaagg aggaatatct
     2881 ttggcaaatg aaatcgtgaa ataccatcat gaattaaata ccattccgtt gcggaaattt
     2941 acaccagttg aaatgaatct atttttctct attgtgtcta gaatgcgcga aaaaggcgat
     3001 aaaacagtcc gtcttacttt tgaccaacta aaagatttaa gtaattataa agcaactgct
     3061 aataatcgtt tcattaatga tttagaaatg acttatgata aattaatgga tttacgtttt
     3121 ggtaaaagaa gtgctaatgg gctcgatata gatagattcg ttatgtttag tgagtttaaa
     3181 atacgtggaa ctgctaaaga tccatatgta gacatacaag tttacgaaaa agctttacct
     3241 ttactaaaca atttagatga gtgggttcgc tattcactcc aacaatttaa cgaattacaa
     3301 agtagctatg caaagactat gtttcgatta ctaaaacaat atcgaactaa aggttttgct
     3361 tatttttcta aagaggattt tcatgaacta ttagatattc caaaaagtta ctggaacaac
     3421 caagcaaacg ttgataatcg tgttataaag cctatacgac aggaattaac cgcaatcttc
     3481 aaaggattat caatcaaaaa gaaatacgga aaagggcgag gaaaacctgt cgttggctat
     3541 caatttacgt ttaaaccaga aatcaaaaaa gcagatgatt ttaataaaca tgcaccaacc
     3601 aaagaagaac aaaataaagc attggcttta gctgataaac aaaaagaata cgaagaacct
     3661 gctacaacaa ttaacgaaaa aatcaaacag aagaaacgag aacttgaact cctccaacaa
     3721 atcaaacaac tagaaaaaga gaaagaagct cttgaaaaac aaacagagtc cgcactttcg
     3781 aaaaaagaga aaaaaagttt attatctgat ttagataatt tattccagta gcaacctagg
     3841 aggcaaccag tatgcaacca caagaaacct ttacaataaa ggaattgtca gaacttttta
     3901 agatgagtag gcaagcaata tcgaaacata tccaaaaatt agattcttcc atgatagcta
     3961 aaaatgagcg tggttataag gttgtattgc gttctggagt attgcaactt gctcgcaact
     4021 taggcaacca aatgttgcta gataggttgc acgagtacga acaagacaac gataaaaatg
     4081 ttaatatacc aagcaaaaac gtggttacaa acaagttgct agatcagttg cgtgaagaaa
     4141 atgaattttt acgagaacaa cttgccctca gagagcaaaa taattctcat atacaaaaac
     4201 tactcgacca gcaacaacaa ctcaccctac aagccaacaa acaaatcgaa aaactgcaag
     4261 aaaaactaca gttgacttat acagaagaag ctccagagaa tgactcaacg actctttctg
     4321 aagcaacaga aacaatagac aaacaaattg atactgaaaa aaaatggtgg caactttgga
     4381 aataactgga gggaacattc atgacaagaa aaaatcttct attcggttct attttactag
     4441 gaattctagg attcagtccg atgttattgt cagtgatcaa taaagggaat aaacatggtg
     4501 aattgaaatt gaatccaaat tattacaaca gtcctaacga agaattgttt gtaaaagaaa
     4561 cagataaata ataaaaaaga ctgctctttc gagcagccag ttaaaaaata atgagtgttt
     4621 attcttgaaa aagcaacgcg cttatgaagc gctaccaaaa atttatcata ttttattaaa
     4681 aataacaacc cagtaaaaaa tgactggtaa aaattcacaa agaaacggaa agaagcaacc
     4741 gcagaaaatc aagcttaaaa agagcttttg acctagacca atcgaaaatc acaaagtgag
     4801 cgcctgtatc tattgctaca ggcgctactt ggcatagtat acattatgtc aacaagaatt
     4861 taatataaca tattttccta atgcattcta actataccac attatcaaaa atataaatta
     4921 tctttactaa ataaaatatt gacaagccta taccttttgt tttatgattt tcacaaagaa
     4981 aacgttttct atatattttt ctgtatcagg aggaaattct atgaaaaaat ttactttagg
     5041 ggtagctttg ttaactattg gggcagttgt tttaccgtca actactatag tttatgcttc
     5101 agaaaattcc caagtggtat cattaaacga aaacaatgct actgaaaatg tagttattga
     5161 gtacactgaa gatggtatgg tcgtaactaa atatgaggat ggttatagtg cggaagattt
     5221 tgcaaattct tcagatatca tgacaagagc tgcagttgca agatggggca aaaaggaata
     5281 tacaaatatt gctgtaacca ctggagtagc tgctaatgct attaatgctg ccttttgggc
     5341 aggagggaaa gctagcactt ctatgtttgg tatcccaggt tgggcaattg gtgctttgtt
     5401 aacaggtgca agttggacta aaaaaggtgc ttctccaggg aatgcagttg caaaagaatg
     5461 ggacaaaaat ggtaatggct ggattggatt ttattaccaa aggggatatg atgcagctgg
     5521 aagagttgta gctactagat attcaacgtt ataagttaag gagtgatgtt tttgatatct
     5581 aaatcattaa aatcattaaa aacatttact aaaaagaatc cgcaaatagc tactattcgt
     5641 atttttatat ttgctatagc tgttgctata attgttaata tgctaagcgg agggcaatta
     5701 tacaaatctg gtttattttt agtgttaatc ttagttgtct tagacataat aaagtctaaa
     5761 aaaaaataaa tcgatacatt ttagaagaat agggttgttt gaatattaca aagcaacaat
     5821 ttcaccttcg gtgaaataag cgaaacttta caaaaagtgg ctagcaaatg ctagcctttt
     5881 tttgtattgt ggagcgtgga ttttgagggg gattcaaggg ggagcttgcg ctcctttgac
     5941 aagcggttat ttgcaccaaa tacggaggta tttggctagc aaatatagag cttgccaaac
     6001 ccttttagtg agctttactc attgtacctt tttaggtaca atggtattgc gaacgatacg
     6061 tccgtaggac gtactgaaag ggtgggactt atgagcgaac aacatcaaaa tttggctagc
//
LOCUS       DARBEC010000119         6002 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.125, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000119 DARBEC010000000
VERSION     DARBEC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6002)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6002)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6002
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.125"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..395
                     /locus_tag="SCL80_002560"
     CDS             <1..395
                     /locus_tag="SCL80_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108398.1"
                     /translation="ISLKDESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQE
                     YYFYKCITKRVCCICGKTGADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKI
                     GVINFKNKYQIKGIKLSHETIKKLRIGG"
     gene            398..1228
                     /locus_tag="SCL80_002561"
     CDS             398..1228
                     /locus_tag="SCL80_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEG1108399.1"
                     /translation="MAEISWIKLKTTMFDDEKIRLIQAVPESDAIIVIWIRLLVLAGK
                     TNDDGLIYIQRNMPYTEEMLATLFGKNVNTVRLALTTLANFNMIDLSSDGLIAISNWE
                     KHQNIEGMDKVRLKNAERNRKYRERKRQERLKLENDVSVTSRDGTDKDIEEDKDIDKE
                     EKKGKYSDEHLRLAKKMQRNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEYV
                     INWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKKNHKKQSQKLQYSNPSEYDDLPI
                     "
     gene            1244..2095
                     /locus_tag="SCL80_002562"
     CDS             1244..2095
                     /locus_tag="SCL80_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1108400.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSYVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            2092..2451
                     /locus_tag="SCL80_002563"
     CDS             2092..2451
                     /locus_tag="SCL80_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEG1108401.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCSQYRV
                     PLILAKKYRNTFKEERF"
     gene            2466..2627
                     /locus_tag="SCL80_002564"
                     /pseudo
     CDS             2466..2627
                     /locus_tag="SCL80_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
     gene            2624..2929
                     /locus_tag="SCL80_002565"
     CDS             2624..2929
                     /locus_tag="SCL80_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108402.1"
                     /translation="MSVDKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWAEKPK
                     RKTPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            2929..3240
                     /locus_tag="SCL80_002566"
     CDS             2929..3240
                     /locus_tag="SCL80_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEG1108403.1"
                     /translation="MNELITKVEQWAKDKGLDQADPKAQFLKVAEEFGEIASAMARSN
                     DELFKDSVGDVIVTLIILSMQKGANIQECLEMAYNEIKGRTGKMVDGVFVKSSDLEDS
                     E"
     gene            3237..3410
                     /locus_tag="SCL80_002567"
     CDS             3237..3410
                     /locus_tag="SCL80_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108404.1"
                     /translation="MMMPKEIYSDALRGARNQLKMAKRAYEIRPTIENERRVKAIRRR
                     CSIYGELQKEDSK"
     gene            3407..3823
                     /locus_tag="SCL80_002568"
     CDS             3407..3823
                     /locus_tag="SCL80_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YopX family protein"
                     /protein_id="HEG1108405.1"
                     /translation="MIPRFRAWDKRKNVMRDVAVLHFTKNGKTNFIEYWINPTELKSY
                     HVRNIELMQSTGLKDKNGIEIFEWDIVSVSVRNGFDYLDNKVCVVKNSIGHSGLVCAT
                     VDEDLEYQIFNTELFEEYTYEVIGNIWENSELLEEQ"
     gene            3823..4491
                     /locus_tag="SCL80_002569"
     CDS             3823..4491
                     /locus_tag="SCL80_002569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1642 domain-containing protein"
                     /protein_id="HEG1108406.1"
                     /translation="MNKQELIDELAKYVKRYENVMDEHGQGRYGAYEVSLKLVKRLNE
                     SKITDEQAWNKVAEAYPESAQSLRNTLDNAVFGKTGEPQKHVMPKFVAEWIEYAKKKG
                     DSLAISFKPWNLYGVEYSKADRWIGDNQETFARAWLDGYEVEKEPLYYVPLPYEVWDE
                     EAAELKTEYLYLHYEITSDETRIFPTKEPRKGFVAKLDELTIKSADENYWPFAVPVEE
                     VVEG"
     gene            4488..4865
                     /locus_tag="SCL80_002570"
     CDS             4488..4865
                     /locus_tag="SCL80_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108407.1"
                     /translation="MKKLHQLISEKESELQNLEDSLGLGFPIVEQVKMVQISHLQLEL
                     EDLRQIEDPYQLNDNQQIVLEWLKLTASTGKPMQVVFWMMNNAAWGHLDELRDPLMEL
                     TDKQQFEVLTAFAQWGLEQEEKE"
     gene            4862..5161
                     /locus_tag="SCL80_002571"
     CDS             4862..5161
                     /locus_tag="SCL80_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108408.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKTRKKPKVKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKLLAKSKDNLAVVRLVDMEVINNEI"
     gene            5151..5309
                     /locus_tag="SCL80_002572"
     CDS             5151..5309
                     /locus_tag="SCL80_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108409.1"
                     /translation="MKFKIFEEDTRYKLEKKLNEFAKNNEIQHISLTASKSGYTTYYA
                     AVVSYVIQ"
     gene            5403..5819
                     /locus_tag="SCL80_002573"
     CDS             5403..5819
                     /locus_tag="SCL80_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autolysin"
                     /protein_id="HEG1108410.1"
                     /translation="MQLLREVDFKQTRCNARDVLKNFRRLERMAGRSLIDIKSPIITD
                     MPKAPKHGNKAEDAIIQMMDIEAERDAILAALMALSLISRQILYYSFCDVNKHSNYEI
                     GQLIRGYGEKNVEKLKSIALIEFAEAYKKGVLVQYR"
BASE COUNT         2231 a          899 c         1391 g         1481 t
ORIGIN      
        1 aaataagcct caaagatgaa tcgaatacga ctgtgagtga tgctaatgtc ttagcaaata
       61 tcatcttaga tttcatcttt gaacatcata ttcctttcaa agaaggctat gagattttac
      121 cagcgaatca agaatattac ttctacaaat gcattacaaa aagagtttgt tgcatctgtg
      181 gcaaaacagg agctgacatc gatcactttg acaaagcgct aggaagacga aagcgaaaag
      241 aagttgatca ttcagagtac acatttgcag cactctgcag aatccatcac acggagaagc
      301 acaaaatagg tgtgatcaat tttaaaaata aatatcaaat caaaggaatc aagttaagtc
      361 atgaaacgat taaaaagtta aggataggag gataaatttg gctgagataa gttggatcaa
      421 acttaaaact actatgtttg acgatgaaaa aatacggtta atccaagctg ttcctgagtc
      481 ggatgccatc atcgttatat ggattcgatt actagtttta gcaggaaaga ctaacgacga
      541 tggtctgata tatatccaga ggaacatgcc ttataccgaa gaaatgcttg ctacattgtt
      601 tggcaaaaac gtaaatacgg ttcgcttagc gctaactaca ttggcaaatt tcaacatgat
      661 tgatctaagc agtgatggac taattgccat cagtaattgg gaaaaacatc aaaatatcga
      721 gggtatggat aaagtaaggc taaaaaatgc tgaaagaaac cgtaaataca gggaaagaaa
      781 gagacaggaa cgtctcaaat tggaaaatga cgttagcgtg acgtcacgtg acggtacaga
      841 taaagatata gaagaagata aagatataga taaagaagaa aagaaaggta agtattctga
      901 cgaacactta cgccttgcta aaaaaatgca aaggaattta actgaagatt ttccaaaaga
      961 aatgaacaaa gtagatatcg aaaaatgggc agacacaatc aggttgatgg aagaaagaga
     1021 taaagcatct atagaagcga ttgagtatgt gatcaattgg ctacctacaa atgaattttg
     1081 gtttggaaat attagaagtg ctaagaaatt gagagaaaaa tttgagaagc tcaaattcga
     1141 aatcaaagca gacaagaaga atcataaaaa gcaaagtcaa aaactacagt acagcaatcc
     1201 tagtgaatat gacgacttgc caatttaaaa ggagatgcat cacatggaaa gcctagcaaa
     1261 tgctatggag aagctaataa gaagagtatt agtgcaaagc ggaaaatgtc cagaatgtag
     1321 cgaacctttg tatagttggc gagctaaaaa taaggatggt tcagaacgtt gtaaaccaac
     1381 atgtatgagt tgtggttata aagcgttacg tgtgaaagag gatatacaga ccgaacggat
     1441 atataacgac agcttaaaag cacgagcatt gagttttttt caaaatggtt cggtattaac
     1501 agataaaact ttgtttaaat gcaaaatgga gaattatcac gtagtggacc aagaaacgaa
     1561 aattgcttta gaaagagcta aaagctatgt aaatgatgtc ctactgaacc atcctgcaca
     1621 tttcattcta tcagggaaat caggaagcgg aaaaagccac ttatcaatgg cgacagcttg
     1681 ggaaatactt gagcgctcaa attatgacaa gaaaatactt tttataagct atcaagagtt
     1741 attagagcaa ataaagtttt cttataacaa tgctgaactg agaaaagaaa ttgaaggatc
     1801 gcttatagcc gatatcaaaa caactgattt ggtggttttt gacgatattg gagctgaatt
     1861 aggtagcggg gtatcaaata gtaggcaatt tacaaacaac acgttaaaca cgctcttaga
     1921 agccagacag aacaaggcaa cgatcatcac aacaaactta tctggtcctg aactaagaga
     1981 agcctacggt gaaagaattg tttctaggat atttaagaat tcagaaggtt atgcgctgaa
     2041 attccaacaa acagcagaca agcgcataaa accagtgaaa ggtagtatcg catgaataaa
     2101 taccgtaata aaaaaactgt tcatcgaggt atcaagtttg attctatcgc agaagcagag
     2161 tattacgatc tagccttgtg gcaagctgaa gcgaacggct ggaaagtaaa acttcaggaa
     2221 agatttgagc tgatgccgaa atttgaacta gacggaaaga agtatcgcaa gatcgagtat
     2281 attcccgact tcacatttta taaaaacggc aaactagtca aagtcgtaga tgttaaaggg
     2341 atgcagacaa aagatttcaa aatcaaagcg aaactcttct gcagtcaata tcgagtacca
     2401 ctgatcttag cgaagaaata tcggaatacg ttcaaggaag aacggtttta acgaggtgat
     2461 ccatcatgac aacagaagaa gtgattcaaa tgcgtattcg aagccttcag cgtgagattg
     2521 acgatctgta acggacaaag gcagtgatgg tcaatgaaac ggcgaggaag gcaatcgatt
     2581 tgcacataga aaatttaaga aggaaaatcc atcgattgga ggaatgagcg tggataagaa
     2641 agcagcaatg aaacgaatca tcgaactgac acattctgag aattggcaag aagacaaaga
     2701 aatagttgca gaagtccaaa agctcggtaa atcaatgtgg gctgaaaagc ctaaacggaa
     2761 aacgccgaga aaaattgcaa tctggcatgg tgatcgaatt ctagtaacag gtactgctga
     2821 acagttatct gaaattactg gattaagcaa aaacattatc tgggatagag ctaggagctt
     2881 atggattgat tcaaaaggac gacagtttag gtatgtggag gagaaataat gaacgaacta
     2941 atcacaaaag tagagcagtg ggctaaagac aagggactgg atcaagcgga tccaaaagca
     3001 caatttttga aagtagctga ggaattcgga gaaattgcat cggcaatggc aagaagtaat
     3061 gatgagctat ttaaagatag cgtaggagac gttatcgtca cgctgattat tctttccatg
     3121 caaaaagggg caaacataca agagtgttta gaaatggcgt ataacgaaat caaaggacgc
     3181 acaggaaaaa tggtagatgg tgtattcgtg aagtcgagtg atttggagga cagcgaatga
     3241 tgatgccaaa agaaatctat tcagatgcat taagaggtgc tagaaatcaa ttgaaaatgg
     3301 caaaaagagc atacgaaatt cggcccacaa tcgaaaatga acgcagagta aaagcaattc
     3361 gccgtagatg ttcaatttac ggcgagttac agaaggagga cagcaaatga taccgagatt
     3421 tcgagcttgg gataaacgaa agaacgtaat gagagatgta gccgtcttgc attttactaa
     3481 aaacggcaaa acaaacttta ttgaatattg gataaatcct accgaattga aatcatatca
     3541 cgtgcgaaac atcgagctca tgcaatcaac agggctgaaa gataagaatg gcattgagat
     3601 tttcgagtgg gatattgtat cagtcagcgt gcgaaatgga ttcgattatt tagataataa
     3661 agtttgtgtt gtcaaaaatt caataggaca ttcgggatta gtgtgtgcca ctgttgatga
     3721 agatttagag tatcaaattt ttaacacaga gctgtttgaa gaatacacgt atgaagtcat
     3781 cggaaatatt tgggagaata gcgagttatt ggaggaacag tgatgaataa acaggaattg
     3841 attgatgaat tagctaaata tgtaaagcgt tatgagaacg ttatggatga gcatggccaa
     3901 ggaaggtacg gcgcttatga agtatcttta aagttggtga aaagactaaa tgaatcaaaa
     3961 attacggacg aacaagcttg gaataaggta gctgaggctt atcctgaatc ggcacaaagc
     4021 ttgagaaaca ctttagataa tgctgtattt ggtaagactg gtgaaccgca gaagcacgtt
     4081 atgccgaagt ttgtggcgga gtggatagaa tacgctaaaa agaaaggtga tagtctagct
     4141 atttcattca agccgtggaa cctctatggt gttgagtata gcaaggctga tagatggatt
     4201 ggagataatc aagaaacgtt tgctcgtgct tggctagacg gctacgaggt cgagaaagag
     4261 ccgttgtatt atgtcccgct accatatgaa gtttgggatg aagaggcagc ggagcttaaa
     4321 acagagtatt tgtatctaca ttatgagatc acaagtgatg aaactagaat atttcctaca
     4381 aaagaaccta gaaaaggttt tgtcgcaaaa ttagatgaac ttacgattaa atcagcagat
     4441 gaaaactact ggccgtttgc tgtaccagtg gaagaggtgg tagaaggatg aaaaaattgc
     4501 accaactcat atctgagaaa gaaagcgaac tccaaaattt agaagatagt ttgggattgg
     4561 ggtttccaat tgttgagcaa gttaaaatgg ttcaaatcag tcatctacaa cttgaattgg
     4621 aagatttaag acaaatagaa gatccgtatc aactcaacga taaccagcaa atcgtgttgg
     4681 agtggttgaa actaactgcg tcaacaggaa agccaatgca agttgtattt tggatgatga
     4741 ataatgcagc atgggggcat ttagacgaat tgagggatcc tttgatggaa ttaactgaca
     4801 aacagcaatt cgaagtccta acagcattcg ctcaatgggg attagaacag gaggaaaaag
     4861 aatgaaacta aaagacggat tttacgctag tagccacggt atcggcggtt taatgctaga
     4921 catgccgaca aagaacccta aaacacgtaa gaagccaaaa gtcaaagtcg gtgacatggt
     4981 tcgctgtgaa gcagaaggat tcatctatcc atttcgtgga tatgtagaac acgtctataa
     5041 tcactcagcg atcattcgca ttgaaaacac gatggaatgc gacaagttat tagcgaaaag
     5101 caaagataat ttagcagtgg tgagattggt ggatatggag gttataaaca atgaaattta
     5161 aaatctttga agaggacact cgctataaat tagaaaaaaa attaaatgaa tttgcaaaga
     5221 ataatgaaat tcagcatata tctttaacgg cttctaagtc aggttataca acttactatg
     5281 cagctgttgt gagctacgta attcaataaa aggaactcgg caaataaaaa agccggatca
     5341 ctccgactga tgtaataaat ccgacaagtt tattatatca cataaaggag cggtttgact
     5401 tgatgcaatt gttacgagag gtagatttca aacagacaag atgtaatgcg agagatgtgc
     5461 tgaagaactt tcggcgcttg gagcggatgg caggtcgctc tttgatagat attaagtcgc
     5521 cgattattac ggatatgccg aaggcaccga agcacggcaa taaggcagaa gacgcgatca
     5581 ttcagatgat ggatatagaa gcggagagag acgcgatttt agcggctttg atggcgctta
     5641 gtctgattag ccgtcagata ctctactaca gcttctgtga cgtaaacaag cactctaatt
     5701 atgaaatagg gcaattgata cgaggatacg gagagaagaa tgtagagaag ctgaaatcca
     5761 tcgcattgat cgaatttgct gaagcataca aaaaaggcgt gttagttcag tatcgttaat
     5821 tttgtagggt ttttgtaggg atagtgtagg gtttttgagc ggtttaacgt gatattatga
     5881 tagtgtcgaa agattagtga taggtctaag acaaaataat aataaaagga acatcgtttt
     5941 attattgttt cacaattaag cttcgataga cagcagcgga aatattaaga ataaggatgt
     6001 ga
//
LOCUS       DARBEC010000120         5990 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.126, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000120 DARBEC010000000
VERSION     DARBEC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5990
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.126"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(480..767)
                     /locus_tag="SCL80_002574"
     CDS             complement(480..767)
                     /locus_tag="SCL80_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1108411.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(787..1011)
                     /locus_tag="SCL80_002575"
     CDS             complement(787..1011)
                     /locus_tag="SCL80_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEG1108412.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(1466..1636)
                     /locus_tag="SCL80_002576"
     CDS             complement(1466..1636)
                     /locus_tag="SCL80_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108413.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(1651..2184)
                     /locus_tag="SCL80_002577"
     CDS             complement(1651..2184)
                     /locus_tag="SCL80_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1108414.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(2205..3149)
                     /locus_tag="SCL80_002578"
     CDS             complement(2205..3149)
                     /locus_tag="SCL80_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepB family plasmid replication initiator
                     protein"
                     /protein_id="HEG1108415.1"
                     /translation="MANEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMREKGDKTVR
                     FTFDQLKDLSNYKATANVRFVEDLKATYEKILSLRFGRRSESGLSYDMFVMFTEFKIN
                     GDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQFNELQSGYAKTMFRLLKQYRTKGFA
                     YFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEDQNKALALADKQKEYEEPATTINEKIKQKKRELELL
                     QQIKQLEKEKKALEKQTESALSEKEKKSLLSDLDNLFQ"
     gene            3786..3872
                     /locus_tag="SCL80_002579"
     CDS             3786..3872
                     /locus_tag="SCL80_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1108416.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(3993..4682)
                     /locus_tag="SCL80_002580"
     CDS             complement(3993..4682)
                     /locus_tag="SCL80_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108417.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(4927..5841)
                     /locus_tag="SCL80_002581"
     CDS             complement(4927..5841)
                     /locus_tag="SCL80_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEG1108418.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(5823..>5990)
                     /locus_tag="SCL80_002582"
     CDS             complement(5823..>5990)
                     /locus_tag="SCL80_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEG1108419.1"
                     /translation="QAIARDLAKAGGNINQIAKWCNTHQHDVDPGDAQRLSENLKIMQ
                     KELQKIWQQLK"
BASE COUNT         1595 a         1107 c         1000 g         2288 t
ORIGIN      
        1 gctagccaaa tattgatttt gttcgctcat aagtcccacc ctttcagtac gtcctacgga
       61 cgtatcgttc gcaataccat tgtacctaaa aaggtacaat aagtaaagct cactaaagct
      121 cactaaaggg cttggcaagc tctatatttg ctagccaaat acctacgtat ttggtgcaaa
      181 taaccgcttg tcaaaggggc gcaagctccc ccttgaatcc ccctcccccc cactctccac
      241 aatacaaaaa agactagcaa atgctagcca ctttttgtaa agtttcgctt gtttcacctt
      301 cggtgaaatt gttgcaaatc aataattagg tagagctaca tttttttcgt tgttcaaaga
      361 ttcttatgcc atttactatt aataaatcta taaatatgat aatttttcgg ctctataatt
      421 tatgggactg atgaatcaga attgattctt cagtcccatt ttcattttat tccaaagaat
      481 taactccaaa taccaataga agcccatcct ctatatactt gagattcctt actaacaatt
      541 tcttgtaatt ctattaaaga tttaggtaat ccaaatttat gttttaaaat atatcttcca
      601 agaaaactac ttaatttcat actaacttca ccaacacttt ttccctcact gatttcttta
      661 gaataaagaa agagcttttc ttttaagtca ggatagtcat ctatagttgc ttcattgtat
      721 actttactaa ttgcatttaa aagtttttct tctttagtaa aatccattat aataacctcc
      781 ctaccactac cttctaggtg cccatggacc atgattaacc caaccattaa caataatttt
      841 tccaatttct cctttagctt gattccaatc tacccaacat ttttctttgt tacaataaag
      901 accatttcca tagtaagtag ctgcatcaac ttttattcct gtaccgatgc cagctagaca
      961 agttcctaat attcctagaa taacacaatg ttttaatact ttctttttca tgattgtttt
     1021 tccttttcgt ttttttaaaa ttgcttgtta tattaaataa tcttagtaat tttattatat
     1081 aacattttat tatataacaa tgattaacgt cataaaaagt agttttgaat tcataatcgt
     1141 ttaaataaaa ttataagatc agttatttaa acagctagta gacataatgt acactatgcc
     1201 aagtagcgcc tgtagcaata gatacaggcg ctgattttgt gaatttttat tggtctaggt
     1261 taaaagctct ttttaaggct tggttttctg cggttgtttc tttctaattc tttgtgaata
     1321 ttgatcggtc attttttaaa tacttgttat ttttgtttgt gtttgttatg ctttttacat
     1381 atctcatatt catttattat tcttttctaa ggttaataat aggataatct ctttcagaaa
     1441 gactgctcgt acgagcagtc ttttcttatt tatcaagttt ttctacaaat acttcctcat
     1501 tttgactgtt gtagtaatta ggattgagtt ttagttcccc ttttttattt cctttgttgg
     1561 ccactgataa taacattggg ctaaagctta atattcctag taggatagaa ccgaatagca
     1621 agtgtttttt tgtcataagt atttccttct tcatttccat agctgccacc attttttcca
     1681 attctttgtt tgttcgtcca ttgtttctgt tgcttcagaa agagtcgttg aactattctc
     1741 tgaagactct tccgtataag tcaactgcag ttgctcctga agtttttcga tttgttggtt
     1801 ggcttgcagg gtgagttgtt gttgctggtc gagtagtttt tgtatatgag aattattttg
     1861 ctctttgagg gcaagttgtt ctcgtaaaaa ttcattttct tcacgcaact gatctagcaa
     1921 cttgtttgca accacgtttt tgtttggtat ataagcattt ttatcgttgt tttgttcatg
     1981 tttatgcaac ttatctagca acctttgatt gtctaagttg cgagcaagtt gcaatactcc
     2041 agaacgcaat acaaccttat aaccacgctc atttttagct atcatggaag aatctaattt
     2101 ttggatatgt ttcgatattg cttgcctact catcttaaaa agatctgaca attcctttat
     2161 tgtaaaggtt tcttgtggtt gcatactggt tgcctcctag gttgctactg gaataaatta
     2221 tctaaatcag ataataaact ttttttctct ttttccgaaa gtgcggactc tgtttgtttt
     2281 tcaagagctt ttttctcttt ttctagttgt ttgatttgtt ggaggagttc aagttctcgt
     2341 ttcttctgtt tgattttttc gttaattgtt gtagcgggtt cttcgtattc tttttgttta
     2401 tcagctaaag ccaatgcttt attttggtct tctttggttg gtgcatgttt gttaaagtcg
     2461 tctgcttttt tgatctcagg tttgaacgta aattgatagc caataacagg ttttccccgt
     2521 ccttttccgt atttcttttt gattgttagt cctttgaaga ttgcagttag ttcttgacgt
     2581 ataggtttta taactcgttt atctatatca ggttgcttat aacttttagg aatatctaat
     2641 aattcaagaa agtcttcttt ggagaaatat gcgaaacctt tggttctgta ttgctttaac
     2701 agacgaaaca tagtctttgc atagccactt tgtagctcgt tgaattgttg taaagaatag
     2761 cgtacccact cgtctaaatt atttaacaaa ggcagtgctt tttcgtaaat tcgtatatct
     2821 acgtaaggat tttctacgtc cccgttgatt ttaaattctg taaacattac aaacatatcg
     2881 taagaaagcc cagactcact tctacgacca aagcgtaaag ataagatttt ttcataagtt
     2941 gcttttaaat cttctacaaa tcgtacattt gcagttgctt tgtagttact taaatctttt
     3001 agttggtcaa acgtaaaacg aactgtctta tccccttttt cacgcattct agacacgatg
     3061 gaaaaaaaca aattcatttc aaccggtgta aattttctta aaggaattgt atttaattca
     3121 tggtggtatt ttacaatctc attagccata taatcacctc aatccaatta tgaatgacat
     3181 tttttaaatg tcaagacatt catatttgac gttcaaacag acaaaatgtc cttcataaac
     3241 agacaaaatg tccttcataa acagacaaaa tgtccttcat aaacagacaa aatgtccttc
     3301 ataacattct gtatccattg gtagagtaag gacacagctt acggtaaaga ggtttaaaaa
     3361 gaggtttaaa aagagatata aaaagagggg ggctaatttt cccgcctaaa aaccacttct
     3421 tttttcttta aacaggttcg cgcatttttc aaaactgcca atccaaaacc ttgcttccta
     3481 cggtcgccaa aaaggtgtga tcgctacgct ctcacattgt actgcgcagt cgcctaaagg
     3541 ctcctttgct aaaaacatgt tggtaaagag gcctttgagc ggttctgaat gcttatctaa
     3601 gccgttttaa gacagtcttg gataaaaatc cttatctagc tttaaagcgc cttacaggcg
     3661 gtcagggcaa gccctaacaa ccctaaaata agcgatactt aaaaacacca cacaagttta
     3721 attttttaag atataataac ttgtcgccta tccgacagtc ttgcgactgg aaggaggtgt
     3781 atctaatgtt tgaattgttc atttgccccc ttcttataga agtttgtgtc tctttattca
     3841 gttattggtt gaataaccga aacaagaagt aaaatcaata aaggcgacat caacctgaaa
     3901 aaaagcccca gagaatccga cctctctggg gctttcgtgt atctaatgtt tgatcatttg
     3961 catgtttagt ttaacatgat tgccgatcaa attcaatttt gctgttgctg atctaaccat
     4021 tccttgacac tttttagata atttaatgcc gtttcttttt tcgttggatt ttgtatgttc
     4081 gttgcttgct gaagcagtgc tttataacgt tcgtaggtaa ctgattgttg ccgttctttt
     4141 cgttcctgtt cactagttga aaaagggatt gtatcactga ataaattata acaagcttct
     4201 tctgtctgcg tatctttaaa atattgcacg tcttttttgg ttcgctcata gttgtcttga
     4261 acctcttttt gctgtttttc tggcaagttt tgataaagac gagtgagttt ttccatgttt
     4321 ttatccgtca aacgtttttc ttgattcaca aaaatatcgt tcatttcctc ataacaagtg
     4381 gtaaactctt ccattttttg tgttaacact tgctcctttt tctttttatc acatgctgct
     4441 tcatactttt gagtgatatc ccctaataaa atacttcttg aaatacgttt atttccctct
     4501 aaaacgccga ataaaccact taaaacgatt gataatgtaa atataacccc tggcaataaa
     4561 aacctcttaa atcgaatttt agagcctttt aatgaatatc ttgtttgata agccttatag
     4621 ctaagccaaa taattcctaa aatactggca aaaatgagta gatagggtaa atagaatgcc
     4681 atttctattt attctcccca taccagccaa aaggctaaca gtgtcatgat taagcaacat
     4741 aagggggcaa gaatatccat tgatcgctgc ataattactt ttttctgttc tttatttcgc
     4801 tggtttaata gcgcatggtg gaattttttg acttgtgaat caattgtagt ttgtattctt
     4861 tctttggtat aaggtgcaag aaaagcacgt tgtttcttta tttcattttc tatgtcttgt
     4921 aacgttttag aacgataatt cttgttggta gtctgctcct ctttcaacgt gttggtcatg
     4981 gtttcttcga gtcggtctaa tttccggttg ttcagtcgta agagttcgtt gttctcttcg
     5041 tacttctttt gcagtttcgc gaatgctagc tcgagctctt cgtagctcgt tggttcgttg
     5101 ttgattgtag gctcgttctc gtctgtccag ctcatgtaaa atcgtctcct tttcgtaatc
     5161 ctttcctagt ttgttggatc gtactttttt ctttttgtgg atcaattcgt aagtaaaatt
     5221 tttcccacga ttcaagcaat gaatgccgaa tgttttaaga gaatcacaaa attggttata
     5281 cgttcgagtt tttttgtttt tcattgtttg atcaatcgca aaccgaattt cgtctttcca
     5341 aacaggttca tttttttctt ttaatttgat ttcagacatg gttcgttttt cgggtttctt
     5401 ccctcgttca gtgattgtta agtcatgttc aagacaaatc tcgtcattta ggtcataaca
     5461 tttgtttaaa aatgcttgac catgggcttg aaatttgttc ccagtttcca gattgacgga
     5521 gttaatgaca atgtggttat gaatatggtc tttgtctgta tgtgtgtaaa tcgcgatttg
     5581 gtggtcaggg gctatttttt tcgcatactc tgcccctagt tgattggctt tttcagcggt
     5641 aatttctcct ggtcgaaaag actggatcaa taagtgtgct tgtacacgat cgtcttttcc
     5701 atacagcatg cgaatttgtt tcatttcgct tttggcataa tcaatgtcac agtttatccc
     5761 tgttttgacg gttgctcgtg gttcggcgta attgatcgct cggctacaag aagtggttcg
     5821 gcttacttta actgttgcca tatcttttgt agctcctttt gcatgatttt taggttttcg
     5881 gataagcgtt gggcgtcgcc aggatcaacg tcgtgttggt gtgtgttgca ccacttggca
     5941 atttgattga tattcccgcc agctttggct aaatcgcgcg cgattgcctg
//
LOCUS       DARBEC010000121         5809 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.127, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000121 DARBEC010000000
VERSION     DARBEC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5809)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5809)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5809
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.127"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            450..1604
                     /locus_tag="SCL80_002583"
     CDS             450..1604
                     /locus_tag="SCL80_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase C47"
                     /protein_id="HEG1108420.1"
                     /translation="MIILKPKNKIYLGLLFSLMIGIFSIGITNTYASENSNFNINSQP
                     IEEQAINFAKTDFEHSITSILLMENNTETDINYTLGSPFKMLDGENTANSSIFFPVFS
                     DADNTIKYVYTVKKNTNGEYSGSISQFLAKELQNLVDSNITDTTLFFQDNSIYYSDNG
                     QIKLLWKSPLSTEKNGVSSFTEKNDLISVNIKNKIKNFSKKQNFPAVKRVKSVSSGDN
                     FIMINWRIAETQTSFPWCAAYVEAAILCNKTDVRATSARAIMHYTYPNLNNSQIESKS
                     LNQDQIIKYANYCGVYPKKVARILNLSEVQTQLRKGNAIYMGTAGYGNYSNSRHALAL
                     FGWVNQGGYQTYYVWNPWYNYPTVISANAGPVTIPVPGGGFTWDNTITNW"
     gene            1598..2020
                     /locus_tag="SCL80_002584"
     CDS             1598..2020
                     /locus_tag="SCL80_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108421.1"
                     /translation="MVNKPIVLVMGLVLVLFLTSCSKKQEIQKTELIPFLGTWQTKDH
                     SERLVITNLNTSKNIEQYTILVKENNHKIQIIDKNLNINEQVFIYKATNNLLTLRSNK
                     KQLDYTFSLDSPKKLVFILTTNDGTEGTAKPIEFFKAD"
     gene            3143..4075
                     /locus_tag="SCL80_002585"
     CDS             3143..4075
                     /locus_tag="SCL80_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108422.1"
                     /translation="MANEIVKYKPELNTIPLRKFTPIEMNLFFSIVSRMRDKGNQKIR
                     FYFDQLKDLSAYKQTAGEVFIKDLTRTYDHLMDLRFGKKSNGGLTIERFTMFTDFEIH
                     GEADNPYVDIKVHEKALPLLNNLESWVRFSLTEFRDLNSSYAKTMFRLLKQYRTQGWA
                     EFSKEDFLELLDIPKSYRQTNINQFVLKPIKEELTPLFKGLTIRKKYGKGRGKPVIGY
                     RFTWKAEINHADDFSKGKQEDLRTKLFNIEHNGELTQEEKWRAKDRILDLPLGTHEAN
                     FKKQQQTEKEEVEKQTLSNELKQDLLGNLQNLFD"
     gene            4092..4607
                     /locus_tag="SCL80_002586"
     CDS             4092..4607
                     /locus_tag="SCL80_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEG1108423.1"
                     /translation="MADKTIKDLAEELGVSKTAINKKVSDENRKLWFAKIGNKFVINE
                     IGQKAIKSMFFTENENSNQQPVGEKSKTENHKNANQVSELELFFFLEKQLNQKDIQLQ
                     EKDEQLKQMQKLLDQQQQLTLQANQQIKKLHEQLQLTYAEESSEKEPLSVTEETQKGQ
                     NKEKKWWQFWQ"
     gene            4903..5184
                     /locus_tag="SCL80_002587"
     CDS             4903..5184
                     /locus_tag="SCL80_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108424.1"
                     /translation="MDILVRNINPKIITRVDKRCQELTTKTGEKWSRNKYLKVLIESD
                     FDRQLMNYKKEQFDLLLEKFIDIQTKNTETLNDYVHTNNQLIETLIGGI"
     gene            5186..5602
                     /locus_tag="SCL80_002588"
     CDS             5186..5602
                     /locus_tag="SCL80_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108425.1"
                     /translation="MRKTSVQKHVTLYPSHIDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EKQLNPSQRNQPKNRQKETSQTFTSKNFDPTNFLKR"
BASE COUNT         2227 a          961 c          931 g         1690 t
ORIGIN      
        1 aaaaaaaaag actgctcgaa agagcagtct caagcaaaaa ataaacgaac aaacgcaatt
       61 actcacgaac acaaacaaat acaataaaat acctaaataa tgaaaataag tgataaaaaa
      121 caagggtatt tatagtttaa caagcgctca taaaaataac aagtctttaa aaaatgacag
      181 ataaaaatgc acaaagaatc ggacacagat aaccgcgaaa aatcaagctt tagaaagttg
      241 tttttagcta gaccaatgaa aaatcacaaa gtccgcccct gtatccgttg gtagaggggc
      301 tacttggttt agtgtacatt atgttaacta gctcgttaaa taacgattat gttcccaaaa
      361 caatatttta tgaactataa caaaaatttg ttatcgcata ttgtatattt taattatagg
      421 tgtatatcac ttaattttaa cacaaaggcg tgattatttt gaaacccaaa aataaaattt
      481 atctaggtct cctcttttcc ttgatgattg gaatttttag tataggaatt acaaatacct
      541 atgcttcaga aaattctaat ttcaatatca actcccaacc aattgaagaa caagcaatta
      601 attttgcgaa aactgatttt gaacactcca taacttcaat tcttttaatg gaaaataaca
      661 ctgaaactga tattaactat actcttggtt cgccatttaa aatgctcgat ggagaaaata
      721 cggcaaatag ttctattttc tttccagtat tttcagacgc tgataatacg attaaatatg
      781 tttataccgt caaaaaaaat acaaatggag aatattcagg aagtatttca caatttttag
      841 caaaagaact tcaaaatcta gtagatagca atataacaga tacaacctta ttttttcaag
      901 ataatagcat ttattattcc gataatggcc aaattaaact actttggaaa tcacctttaa
      961 gtacagaaaa aaatggtgtc tcttccttta cagaaaaaaa tgatcttata tccgtaaata
     1021 taaaaaataa aattaaaaat ttctctaaaa aacaaaattt tcctgcggtt aaacgtgtaa
     1081 aaagtgtctc ctccggagat aactttatta tgattaattg gcgtatagca gaaacccaaa
     1141 caagttttcc gtggtgcgct gcttacgtag aagctgctat cttatgtaat aaaacagatg
     1201 tacgcgctac ttcagctaga gccattatgc attataccta tcctaattta aacaacagtc
     1261 aaattgaatc aaaaagtctt aatcaagatc aaataataaa atatgcaaat tactgtggtg
     1321 tttatcctaa aaaagtagca cgaatactaa acctttcaga ggtccaaaca caactaagaa
     1381 aaggaaatgc catttatatg ggaactgctg gttatggaaa ttatagtaac agccgtcatg
     1441 cacttgcatt atttgggtgg gtcaatcaag gtggttatca aacttactat gtgtggaatc
     1501 cttggtataa ctacccaact gtaataagtg caaatgcagg gccagttaca attcctgtac
     1561 ctggaggtgg ttttacttgg gacaatacca ttacaaattg gtaaataaac ccattgtttt
     1621 agttatgggt ctagtgttag ttcttttctt aactagttgt tcaaaaaaac aagaaataca
     1681 aaagactgag ctgattcctt ttctaggaac ttggcaaact aaagatcact cagaacgtct
     1741 agtaatcact aatttaaata cttcaaaaaa tatagaacaa tataccatct tagtaaaaga
     1801 gaacaatcat aaaattcaaa tcattgataa aaatttaaat ataaatgaac aagttttcat
     1861 ttacaaagca acaaataatt tactaacact gcgctctaat aaaaaacaat tagattacac
     1921 ttttagtttg gattctccaa aaaagctcgt atttattttg acaacgaatg atgggacaga
     1981 agggacagct aaaccaattg aattttttaa agctgattga tgaatagttg tttgatatta
     2041 caaaacaata atttcaccga aggtgaaaca agcgaaactt tacaaaaagt ggctagcatt
     2101 tgctagcctt ttttgtattg cggagcgtga gttttgaggg ggattcaagg gggagtttct
     2161 cccctttgac aagcggttat ttgcaccaaa tacgaaggta tttggctagc aaatatagag
     2221 cttgccaaac cctttagtgt gttttgtaga aagggacctt atagtcactt tcacattaca
     2281 aacgatacgt cctatggacg tatcaaaagg gtgggactta tgagcaaatt aaagcaaaat
     2341 ttggctagca atccttctaa aaaatacacc tattgatcag agtcaaaata aatcagtttt
     2401 cgagtgagcg aatccgaatt tgcaaatctg aagctttgca aatgagtgtg ccggcttccg
     2461 taaaagtcta ggtaacatta gtcgatatat acataaggct ctaatttgcg ttttaaggcg
     2521 ttttaaacgt tctggtatgt aatttatacc aaagcgactt taaaacgcct taaaacgaat
     2581 tttataggtc ttgtaggacg tttcagggtt gttagggctt gccctgaccg cctgtaaggc
     2641 gctttaaaaa caagataagg gattttatct agacttgtct taaaacgccg tagatgagcg
     2701 ctcagagctt cttaaaggac tccttacgaa ctgtttttaa gcaaaggagc ctttaggcga
     2761 ctgcgcagtt caatgtgaga gcgtagcgat cacaccttta aagcgaccgt agggagcaga
     2821 ttcccagttt aggaaatttc gcgaacctgt ttggagaaaa aaagtaggaa atttttaggc
     2881 gggaaatttt gcccccctct tttataatct cttttataac ctctttttaa acctctttac
     2941 cgtcggctgt atccttactc tcccaaggcc tgtagctttt ttagggcatg aaattatctg
     3001 tttataggca tgaaattatc tgtttatagg catgaaatta tctgtttata ggcatgaaat
     3061 tatctgtttt aggtattgtt ttaagacata atatacctta aaattatgtc aataaaatta
     3121 gttagactgg agtatcaaat aaatggcaaa tgaaatcgta aaatataaac cagaattaaa
     3181 taccattcct ttaaggaaat ttactccaat agaaatgaac ttgttttttt ctattgtttc
     3241 tagaatgcgt gataaaggta atcaaaaaat acgcttttat ttcgatcagc ttaaagattt
     3301 aagtgcttat aagcaaactg ctggagaagt ctttataaaa gatttaacaa gaacatacga
     3361 tcatttaatg gatttacgtt ttggaaaaaa aagcaatggt ggattaacca tagaacgatt
     3421 tacaatgttt actgattttg aaattcatgg agaagccgat aacccttatg tggatataaa
     3481 agttcacgaa aaagctttgc ctttacttaa caatttagaa agttgggtac gtttctcttt
     3541 aacagaattt cgagatttaa atagcagtta tgctaaaaca atgtttagat tactaaaaca
     3601 ataccgaaca caaggatggg ctgaattttc caaggaagat tttttagaac tattagatat
     3661 tcctaaaagc tatagacaaa caaatataaa tcagtttgta ctcaaaccca taaaagaaga
     3721 actcacacca ttatttaaag gattaacgat tcgtaaaaaa tatggtaaag gccgaggcaa
     3781 acctgttatt ggttatcgtt ttacttggaa agctgaaatt aatcatgctg acgatttttc
     3841 aaaagggaag caagaagatt tacgtacaaa attatttaat attgaacaca atggagaatt
     3901 aacgcaagaa gaaaaatggc gcgctaaaga tcgaatacta gatcttccgt taggtactca
     3961 tgaagctaat tttaaaaaac aacaacaaac tgaaaaagaa gaagtggaaa aacaaacact
     4021 ttcaaacgaa cttaaacaag atttattagg aaatttacaa aacttattcg attaaagtat
     4081 aaggagtatt tatggcagat aaaacgatca aagaccttgc tgaagaatta ggagttagta
     4141 agacagctat taacaaaaaa gtttctgacg aaaatcgaaa actttggttt gcgaaaatcg
     4201 gaaacaagtt tgtgattaac gaaatcggtc aaaaagctat aaaatcaatg ttttttaccg
     4261 aaaacgaaaa ctcaaatcaa caaccagttg gcgaaaaatc gaaaaccgaa aaccataaaa
     4321 acgccaacca agtttccgaa ttagaactct tttttttcct agaaaaacaa ttaaatcaaa
     4381 aagatataca gctacaagaa aaagacgaac aattaaaaca aatgcaaaaa ttactcgacc
     4441 agcaacaaca actcacctta caagccaatc aacaaatcaa aaaacttcac gaacaactcc
     4501 agctaaccta tgcagaagaa tcttcagaaa aagaacccct ttctgttact gaagaaactc
     4561 aaaaaggaca aaacaaggag aaaaaatggt ggcaattctg gcaataaccc aacaattttt
     4621 attcataaaa attgttgggt tactttcttt ttttgctttt acaagcatag cgacatgatc
     4681 tcacgtcgct attttttaaa gagcataagg aaacgcggta tcactttctt acgaaaggtg
     4741 ctaggtaggc tgtgcctacc acgtttctaa gaaaaaaatt tgctatcact ttttcgccat
     4801 tgtggctagc ttatgtatag tatttcggta aaaaacagct atacaaatac tatatttgta
     4861 tgatatacat aaattataca cacataagaa aggacgtttt ctatggacat tttagttcga
     4921 aatatcaatc caaaaattat tacaagagta gataaacgtt gtcaggaact tacaaccaaa
     4981 acaggagaaa aatggagtcg aaataaatat ttaaaagtgc taatcgaaag tgattttgac
     5041 cgtcaactta tgaattacaa aaaagagcaa ttcgatttat tactcgaaaa atttatcgat
     5101 attcaaacaa aaaatactga aacattgaac gactatgttc atacaaacaa ccaactcatt
     5161 gaaacactta tcggaggaat ataacatgag aaaaacttct gttcaaaaac acgttaccct
     5221 ttatcccagt cacattgacg ttttagattc catcatcgaa aaaaatccag atatcaataa
     5281 ctactcggaa gccattcgct ttttatgtat gagttatgaa cctgatacca atacaacaac
     5341 taccagtaac caagccaaac taaatgcgat gggaaaagaa atttctatca tgaccgaaat
     5401 tatcgcagaa tttgctgatt tgtatttaaa agatacaggc gttcttatcg gtacaaactc
     5461 gaaagtatat caagaagtaa aagaaaaaat tgaaaaacag ctaaatccct cccaacgaaa
     5521 tcaaccaaag aatcgtcaaa aagaaacgag tcaaacattc acttctaaaa atttcgaccc
     5581 aaccaacttt ctaaaaagat aaaaaaataa accaggaaat ttttaatttc ctggtttatt
     5641 ttctagagag cggtcttcca ataagatttg caaatcgatc ccaatttgcg taaaaaaaaa
     5701 gctgctcgta cgagcagcta aacaagaaaa agtcagcttg ttttaagggt aaaataagtg
     5761 taacaaattt tgcgaatgca acgatctttt ttattatacg caaacaatc
//
LOCUS       DARBEC010000122         5790 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.128, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000122 DARBEC010000000
VERSION     DARBEC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.128"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(99..1229)
                     /locus_tag="SCL80_002589"
     CDS             complement(99..1229)
                     /locus_tag="SCL80_002589"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEG1108426.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="SCL80_002590"
     CDS             complement(1877..3079)
                     /locus_tag="SCL80_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1108427.1"
                     /translation="MPKIKNIYQDKKTKKWFFRAYLGIDENGRKVQKTKRGYASQKDA
                     KIAYDKYMETHDFNKTIPNQLCSTNQMTFEEFYKDRFVKWYERQVKSQTYENAQFIFE
                     KRMQFFYHFRVRDITSHDIEDWLFELSQTESRNSRKKVNGETLSKSYINRIRGHLKIV
                     LDRAVKEGVIAKNPVEDVSSLPSENKKVEFWEHSEFQKVMNELKDDKIQTHHRKIVYE
                     MLFYTGIRIGELEALSWSNVDFEKNTITIEKTLIYNTKDDWYFSTPKTKCAYRTIGLG
                     KKLSLKLRQWYDLQKRIGNFEYVAQLDGTFTPPYSFANWLKEAAKKAGVKPIKLHALR
                     HSHVAFLIEQNIQPLSIQERLGHANIQITLGTYGHLYAKSDQQVVSAIDNVQEGAILS
                     KNDNLLPV"
     gene            complement(3139..3342)
                     /locus_tag="SCL80_002591"
     CDS             complement(3139..3342)
                     /locus_tag="SCL80_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEG1108428.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="SCL80_002592"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="SCL80_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="SCL80_002593"
     CDS             4879..5691
                     /locus_tag="SCL80_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108429.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
BASE COUNT         1717 a         1189 c          921 g         1963 t
ORIGIN      
        1 aaataaataa tataaaaggt aaataaaaaa gagtggattt ctccactcca tgaaaagccg
       61 actttttggg acgctttttt ttgccaagcc tcacattttt atagccaaac ttcttttgcg
      121 acttgctgca ataattggat tttcttccat tgttcttctt ctgttaaatg gtttccttct
      181 tctgttgaag caaacccaca ttgaggactg atacttagat tttccaatgg tacaaattgt
      241 tgtgcttctt ttattcgttc ttttattttc tccgtatctt ccaaatctgg aaacttagaa
      301 gtaactaatc ctaaaacaac tttactttct ttgttactcc aatgtcttaa aggagtaaaa
      361 tctcctgctc ggtcagaatc gtattccaaa aagaaaccat cataatttgt tgaaaacaac
      421 tcttctgcaa tgggttcata tccaccggaa aacagccaag aagaagcgtg attcccgcgg
      481 cagatatgtg ttgtgatcgt caaatcttct ggtttgtctt ccagtgcgtt attgataatc
      541 tctgtacaaa tatgggccat ttctttcgga tcaattcctt tttgaagaag tgcttctctt
      601 ttagtttgat cagctagaaa cgcccaattt gtatcatcga attgcaggta acggcaacct
      661 ttttcataaa aagcatgaat cgctgctttg taagtagagg acaagtcctc ataaaaatca
      721 gatagatcag gataaatctc cttgattttt gaagaaccat cattagatag gatctcttgt
      781 cgtaaaatca tcgtgggact agggatcgtt gcttttgcaa caaaagtatc atctgtttcc
      841 actacttctt tcagaaaagc aaaatctttt aaaaatgggt gatttggatt gaaagataat
      901 ttatctacta cccgaatatt ataagctggt gctgcttttc cgtggaatgt ttgattatat
      961 ccagtttctg gaacatagcc ttcgatacca tttagttctt ctaaaaaatc aagatgccaa
     1021 aatccacgcc gaaattcacc atctgtaatt cctttgatgc cgactgcttt ttggtcttcc
     1081 actaattttt ttatttcctg gtcttcgatt gcacgaagtt cttcttttga aagtaagcct
     1141 tcttgaactt tttttcttgc ttctttgatc gattgcgggc gtaagaaact tccgacatga
     1201 tctactgtaa atggtctatt tttgctcatt tcactcactc ttttctttgt tttttgtaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga accatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta
//
LOCUS       DARBEC010000123         5406 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.130, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000123 DARBEC010000000
VERSION     DARBEC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.130"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..985)
                     /locus_tag="SCL80_002594"
     CDS             complement(<1..985)
                     /locus_tag="SCL80_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
                     /protein_id="HEG1108430.1"
                     /translation="MTQVHFTLNNEEVQSIIEHSVKDDVSKNILTTVFNQLMENQRTE
                     YIQADDYERSESRQSQRNGYYERDFTTRVGTLELKVPRTRDGEFSPTVFERYQRNEKA
                     LLASMLEMYVSGVSTRKVSKIVEELCGKSVSKSFVSSLTEQLDPMVNEWQNRSLSGTN
                     YPYLMTDVLYIKVREDHRVLSKSCHIAIGITEGGDREIIGFMIQNEESDDTWSIFFEY
                     LKERGLKGTELIISDAHKGLVSAIRKSFTNASWQRCQVHFLRNIFSSIPKKNSKPFRE
                     AVKAIFKFTDIELARTAKNALVGEYIDQPKYTKACEILDNGFEDAFQYTVIG"
     gene            1137..1832
                     /gene="vanR"
                     /locus_tag="SCL80_002595"
     CDS             1137..1832
                     /gene="vanR"
                     /locus_tag="SCL80_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1108431.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1810..2964
                     /locus_tag="SCL80_002596"
     CDS             1810..2964
                     /locus_tag="SCL80_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1108432.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3179..4147
                     /locus_tag="SCL80_002597"
     CDS             3179..4147
                     /locus_tag="SCL80_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1108433.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4140..5171
                     /gene="vanA"
                     /locus_tag="SCL80_002598"
     CDS             4140..5171
                     /gene="vanA"
                     /locus_tag="SCL80_002598"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1108434.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5177..>5406
                     /locus_tag="SCL80_002599"
     CDS             5177..>5406
                     /locus_tag="SCL80_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
                     /protein_id="HEG1108435.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPKRAV"
BASE COUNT         1626 a         1052 c         1224 g         1504 t
ORIGIN      
        1 ttccgataac cgtgtattga aaggcatctt cgaagccatt atccaatatt tcgcaggctt
       61 ttgtatattt aggctggtcg atatattcac cgactaaggc attcttagct gttcgagcga
      121 gttcaatatc cgtaaactta aagatcgctt ttactgcttc tctaaacggt tttgaattct
      181 tttttggaat ggaactgaag atgtttctta aaaaatggac ctggcatctc tgccaacttg
      241 cgttggtaaa tgacttacga atcgcagaca ctaggccttt atgggcatca gaaatgatga
      301 gttctgtccc ctttaggccg cgttctttta agtattcaaa gaagatggac catgtgtcat
      361 cactttcttc attttgaatc atgaagccaa tgatttcacg gtcgccacct tctgttatcc
      421 cgatcgcaat atggcagctt ttagaaagca ctcgatggtc ctcacggact tttatgtaga
      481 gaacatcagt catcagataa ggataattcg tacctgagag tgaacggttc tgccattcgt
      541 tgaccatcgg gtctaactgc tcagtcaggc tagaaacaaa agatttcgat acagattttc
      601 cacatagctc ttcaacaatc tttgaaactt tacgtgtcga aacgcccgaa acatacatct
      661 caagcattga agcgagcagt gccttttcat ttcgctgata acgctcaaac accgtcggtg
      721 aaaattcacc atcacgtgtt ctaggcactt ttaattcgag tgtacccaca cgagtcgtaa
      781 agtctcgctc ataatagcca tttctttgac tctgacgact ttctgaacgt tcatagtcat
      841 cggcttgaat atattctgtt cgttgatttt ccatcaattg gttgaaaaca gtggttaaaa
      901 tatttttaga aacatcatct tttaccgaat gttcaataat actttgaacc tcttcgttgt
      961 tcagtgtaaa atgtacttgg gtcatgtaac gtcctcctgg gtatgttttt gtggttaaaa
     1021 acattgtacc gtaaaagggc tgttacatgg ccttttatct tttacacaat tatatggact
     1081 ttatcctata atctgataaa ttattaaggg caaacttatg tgaaagggtg ataactatga
     1141 gcgataaaat acttattgtg gatgatgaac atgaaattgc cgatttggtt gaattatact
     1201 taaaaaacga gaattatacg gttttcaaat actataccgc caaagaagca ttggaatgta
     1261 tagacaagtc tgagattgac cttgccatat tggacatcat gcttcccggc acaagcggcc
     1321 ttactatctg tcaaaaaata agggacaagc acacctatcc gattatcatg ctgaccggga
     1381 aagatacaga ggtagataaa attacagggt taacaatcgg cgcggatgat tatataacga
     1441 agccctttcg cccactggag ttaattgctc gggtaaaggc ccagttgcgc cgatacaaaa
     1501 aattcagtgg agtaaaggag cagaacgaaa atgttatcgt ccactccggc cttgtcatta
     1561 atgttaacac ccatgagtgt tatctgaacg agaagcagtt atcccttact cccaccgagt
     1621 tttcaatact gcgaatcctc tgtgaaaaca aggggaatgt ggttagctcc gagctgctat
     1681 ttcatgagat atggggcgac gaatatttca gcaagagcaa caacaccatc accgtgcata
     1741 tccggcattt gcgcgaaaaa atgaacgaca ccattgataa tccgaaatat ataaaaacgg
     1801 tatggggggt tggttataaa attgaaaaat aaaaaaaacg actattccaa actagaacga
     1861 aaactttaca tgtatatcgt tgcaattgtt gtggtagcaa ttgtattcgt gttgtatatt
     1921 cgttcaatga tccgagggaa acttggggat tggatcttaa gtattttgga aaacaaatat
     1981 gacttaaatc acctggacgc gatgaaatta tatcaatatt ccatacggaa caatatagat
     2041 atctttattt atgtggcgat tgtcattagt attcttattc tatgtcgcgt catgctttca
     2101 aaattcgcaa aatactttga cgagataaat accggcattg atgtacttat tcagaacgaa
     2161 gataaacaaa ttgagctttc tgcggaaatg gatgttatgg aacaaaagct caacacatta
     2221 aaacggactc tggaaaagcg agagcaggat gcaaagctgg ccgaacaaag aaaaaatgac
     2281 gttgttatgt acttggcgca cgatattaaa acgcccctta catccattat cggttatttg
     2341 agcctgcttg acgaggctcc agacatgccg gtagatcaaa aggcaaagta tgtgcatatc
     2401 acgttggaca aagcgtatcg actcgaacag ctaatcgacg agttttttga gattacacgg
     2461 tataacctac aaacgataac gctaacaaaa acgcacatag acctatacta tatgctggtg
     2521 cagatgaccg atgaatttta tcctcagctt tccgcacatg gaaaacaggc ggttattcac
     2581 gcccccgagg atctgaccgt gtccggcgac cctgataaac tcgcgagagt ctttaacaac
     2641 attttgaaaa acgccgctgc atacagtgag gataacagca tcattgacat taccgcgggc
     2701 ctctccgggg atgtggtgtc aatcgaattc aagaacactg gaagcatccc aaaagataag
     2761 ctagctgcca tatttgaaaa gttctatagg ctggacaatg ctcgttcttc cgatacgggt
     2821 ggcgcgggac ttggattggc gattgcaaaa gaaattattg ttcagcatgg agggcagatt
     2881 tacgcggaaa gcaatgataa ctatacgacg tttagggtag agcttccagc gatgccagac
     2941 ttggttgata aaaggaggtc ctaagagatg tatataattt tttaggaaaa tctcaaggtt
     3001 atctttactt tttcttagga aattaacaat ttaatattaa gaaacggctc gttcttacac
     3061 ggtagactta ataccgtaag aacgagccgt tttcgttctt cagagaaaga tttgacaaga
     3121 ttaccattgg catccccgtt ttatttggtg cctttcacag aaagggttgg tcttaattat
     3181 gaataacatc ggcattactg tttatggatg tgagcaggat gaggcagatg cattccatgc
     3241 tctttcgcct cgctttggcg ttatggcaac gataattaac gccaacgtgt cggaatccaa
     3301 cgccaaatcc gcgcctttca atcaatgtat cagtgtggga cataaatcag agatttccgc
     3361 ctctattctt cttgcgctga agagagccgg tgtgaaatat atttctaccc gaagcatcgg
     3421 ctgcaatcat atagatacaa ctgctgctaa gagaatgggc atcactgtcg acaatgtggc
     3481 gtactcgccg gatagcgttg ccgattatac tatgatgcta attcttatgg cagtacgcaa
     3541 cgtaaaatcg attgtgcgct ctgtggaaaa acatgatttc aggttggaca gcgaccgtgg
     3601 caaggtactc agcgacatga cagttggtgt ggtgggaacg ggccagatag gcaaagcggt
     3661 tattgagcgg ctgcgaggat ttggatgtaa agtgttggct tatagtcgca gccgaagtat
     3721 agaggtaaac tatgtaccgt ttgatgagtt gctgcaaaat agcgatatcg ttacgcttca
     3781 tgtgccgctc aatacggata cgcactatat tatcagccac gaacaaatac agagaatgaa
     3841 gcaaggagca tttcttatca atactgggcg cggtccactt gtagatacct atgagttggt
     3901 taaagcatta gaaaacggga aactgggcgg tgccgcattg gatgtattgg aaggagagga
     3961 agagtttttc tactctgatt gcacccaaaa accaattgat aatcaatttt tacttaaact
     4021 tcaaagaatg cctaacgtga taatcacacc gcatacggcc tattataccg agcaagcgtt
     4081 gcgtgatacc gttgaaaaaa ccattaaaaa ctgtttggat tttgaaagga gacaggagca
     4141 tgaatagaat aaaagttgca atactgtttg ggggttgctc agaggagcat gacgtatcgg
     4201 taaaatctgc aatagagata gccgctaaca ttaataaaga aaaatacgag ccgttataca
     4261 ttggaattac gaaatctggt gtatggaaaa tgtgcgaaaa accttgcgcg gaatgggaaa
     4321 acgacaattg ctattcagct gtactctcgc cggataaaaa aatgcacgga ttacttgtta
     4381 aaaagaacca tgaatatgaa atcaaccatg ttgatgtagc attttcagct ttgcatggca
     4441 agtcaggtga agatggatcc atacaaggtc tgtttgaatt gtccggtatc ccttttgtag
     4501 gctgcgatat tcaaagctca gcaatttgta tggacaaatc gttgacatac atcgttgcga
     4561 aaaatgctgg gatagctact cccgcctttt gggttattaa taaagatgat aggccggtgg
     4621 cagctacgtt tacctatcct gtttttgtta agccggcgcg ttcaggctca tccttcggtg
     4681 tgaaaaaagt caatagcgcg gacgaattgg actacgcaat tgaatcggca agacaatatg
     4741 acagcaaaat cttaattgag caggctgttt cgggctgtga ggtcggttgt gcggtattgg
     4801 gaaacagtgc cgcgttagtt gttggcgagg tggaccaaat caggctgcag tacggaatct
     4861 ttcgtattca tcaggaagtc gagccggaaa aaggctctga aaacgcagtt ataaccgttc
     4921 ccgcagacct ttcagcagag gagcgaggac ggatacagga aacggcaaaa aaaatatata
     4981 aagcgctcgg ctgtagaggt ctagcccgtg tggatatgtt tttacaagat aacggccgca
     5041 ttgtactgaa cgaagtcaat actctgcccg gtttcacgtc atacagtcgt tatccccgta
     5101 tgatggccgc tgcaggtatt gcacttcccg aactgattga ccgcttgatc gtattagcgt
     5161 taaaggggtg ataagcatgg aaataggatt tactttttta gatgaaatag tacacggtgt
     5221 tcgttgggac gctaaatatg ccacttggga taatttcacc ggaaaaccgg ttgacggtta
     5281 tgaagtaaat cgcattgtag ggacatacga gttggctgaa tcgcttttga aggcaaaaga
     5341 actggctgct acccaagggt acggattgct tctatgggac ggttaccgtc ctaagcgtgc
     5401 tgtaaa
//
LOCUS       DARBEC010000124         4855 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.131, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000124 DARBEC010000000
VERSION     DARBEC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4855)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4855)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4855
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.131"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(220..972)
                     /gene="xth"
                     /locus_tag="SCL80_002600"
     CDS             complement(220..972)
                     /gene="xth"
                     /locus_tag="SCL80_002600"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEG1108436.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
     gene            1085..2005
                     /locus_tag="SCL80_002601"
     CDS             1085..2005
                     /locus_tag="SCL80_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1108437.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQEADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            2078..3013
                     /gene="murB"
                     /locus_tag="SCL80_002602"
     CDS             2078..3013
                     /gene="murB"
                     /locus_tag="SCL80_002602"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEG1108438.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            3311..4789
                     /locus_tag="SCL80_002603"
     CDS             3311..4789
                     /locus_tag="SCL80_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108439.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
BASE COUNT         1704 a          779 c          938 g         1434 t
ORIGIN      
        1 atctgcggta ataagcccaa ccaaacgaaa aatatgagga actatttttg tttggctgtt
       61 cttattactt gatgcaggac agctttttca caatctctcg ataaacagtg ttcaaaagcc
      121 agtttctcgg tattatgcca aatttatgca aaatgtggaa agccattttc acaaaatttt
      181 tcttaatact cgaaactaaa cgactttttc acaacctctt tatttcaaat ctaattctac
      241 tggacaatga tcactgccca taatgtccgt atggatcttt gcatcagcta gtcctggctc
      301 taaatctttg ctcgtcaaaa agtaatcgat ccgccaacca gcattgtttt tccttgaatt
      361 gaatcggtag ttccaccaag agtagacacc ttcctgggtt ggataaaaat aacggaatgt
      421 atcgataaac ccgttatcca acagtcgaga aagagccgct ctttcttctg gagtgaatcc
      481 agcatttttt tgattcgttt tccagttttt caaatcaatc ttttgatgag cgacatttag
      541 atccccacac acgatgactg gtttttgttt ttttagattt tccaaataat tataaaaggc
      601 ttcttcccat tctaatcggt aatctaaacg ttttaattcg ctttgagaat ttggcgtgta
      661 acaggttaca agaaagaaat cggaatattc caaagtaatc agacggcctt ctgtatcatg
      721 gatatcgata cccattccgt aagtggcatt caatgctggt tttttagcaa aaatcgctgt
      781 gccggaatag ccttttcgtt ccgcgtaatt ccaatattga taataacctg gaagatctaa
      841 atcaatttgt cctgcttgga gtttcgtttc ctgcagacag aaaaaatcgg cgtctaactc
      901 atgaaaaact tctaaaaaat ttttatttac gatggcacga agcccgttta cgttccaaga
      961 gataaatttc aataaaagca cactccttcg aattaatttc cttacctttg cgatactatt
     1021 gtacctcatt ttagtaaaaa atggtgtaag atgaaagtaa ctaaatgatc caggaggaaa
     1081 gtagatgaag attggtgtaa tcggtgtagg gaatatcgca gaaaaagcat atttaccaac
     1141 ttatgcgaaa aaacaaggaa cacttgattt ttattttgca acaagaaata aagaaacaaa
     1201 agaaagaatt caaaaagagt acgggttcca acatctatat gaaacgatcg atgagctttt
     1261 agaagaaaaa atcgaagcat gtatgatcca cgcagcaaca aaagtacatt atgaattagc
     1321 caaaaaatgt ctagaaaatg gtatccatgt ttatattgac aaaccactga gtgtccatct
     1381 aaatgaagtt cgtgaattgc aagaattagc tgataagaac caaataattt tgatgatcgg
     1441 tttcaaccgg cgctttgctc ctatggtaga agaattgaaa cgtatcccag ataagcggtt
     1501 gatccacctg caaaaaaatc gaatagcggc aaaagaaacg acagagttcg tcctttatga
     1561 tttgttttta catcttattg atacagctgt gtatttatta gatgaaccca ttttgagaac
     1621 aaaataccag ataagagaaa caaaggaatt tttggaatat gccattttac aattagaaac
     1681 acaaaatcaa acggctattt taacgatgga tttagcaagt ggcgctaata tagaaaaata
     1741 tcaagtaaca agtaaaaatg gtacttatga agtaaacgat ctgacccagc ttgtcattca
     1801 agaggctgat ggaaaaaaag tgaaagaatt tggcgactgg acaaacacgc tagtcaaacg
     1861 cgggtttgag ccaatggtag aggccttctt ttctcagatc cgtttaggta aatcggaaac
     1921 agaattgctt aaacaaaaag aagttgtaag aagtcatgaa ttatgtgagg aagtgcttaa
     1981 agatcactac cgtcatcaac tttgaacaag actggcaaga ttgtattaag cgtgatagaa
     2041 taatgaggac gtttgagaaa agaaaggaaa atcctcagtg aataaagaag aaatagttga
     2101 aagcttagcc acgattacta tattgtttga tgaaccattg atgaactata cgtttacgaa
     2161 aacaggtgga ccagctgatg tccttgcatt cccgaaaaaa caagaagaag taaagcaaat
     2221 catcgattat tgccgaatcc atgatatacc ttggatggta ttaggaaatg caagcaatct
     2281 gatcgttcaa gatggcggga ttcgcggtgt agtcatcatg ctgactgaaa tgaaacagat
     2341 tcatgtcaaa ggaacgatgg tcattgcgga agcaggtgcc tcactgattg atacgactta
     2401 tgcagctttg gcagaatcat tgactggatt tgaatttgct tgcggcatcc cgggaagtgt
     2461 cggtggagcg gtttatatga atgcaggtgc gtatggcgga gagatcaaag atgtttttgc
     2521 tgaagtggat cttttgcttg aagatggaac attgaaaact ttgacaaaag acgaaatggt
     2581 attttcttat cgtcacagta aagtccaaga attacgtgct atcgtattgg aagctcgatt
     2641 cagtttgcaa acaggggatt atgaagcaat caaagcaaga atggatgagt tgaccgaatt
     2701 gcgtcaatca aaacagccat tagaataccc ttcatgcgga agtgtcttta agcgcccagt
     2761 aggacactat acaggtcagc tgatccaaca agcaggattg caaggcctga aatggggcgg
     2821 tgcacaagtc tctgaaaaac atgctggatt catcgtcaat atcgatcatg cgacagcgac
     2881 agattatatt gaattgattg cgcacatcca agaagtaatc aaagaaaaat ttgatgtgtc
     2941 tttagaaaca gaagtgcgga tcattggaga aaaacaagca atatcagaaa caagcagaag
     3001 tttagccaat taataagtaa tggaacttaa aataagtaaa agagcaggac ggtggccgtc
     3061 ctgctctttt ttgtggttgt ttacgcaaat ggcgcaaatc ttgtacattt taacacaata
     3121 aaacaaaaaa tatagaatat ttcctcataa atttttccat tttttgcaat attctcaaaa
     3181 aaaagataaa aaatgagaat aactgatgaa aaaacaagca taatccatca taaaatcagt
     3241 ataggtgaaa agatgaactt tcatctgcat atcaagtaat tggtatgagg cattttttga
     3301 ggtgtaggat atgaagttgg attaccttat agaagaggaa gatagagaaa aactgctgct
     3361 agctagatat ttagaatttg agaaaaagaa ttatgtagtg ctgcctaagt tatatgaaac
     3421 attaggtttg tctaaattta aaacaagaaa ttatatcata gaattaaata aagaatttac
     3481 tgaattcaaa acaaagccag agatccaaat ttcagaagaa gcagagatcc atgtcgaacg
     3541 tctgaatttg acggatatca aaaatttaag aagaatctac tttcaacgat caaaaattgc
     3601 tcgactgtta acggagatcg tggattcaaa agtaacgatc gaaaaatttg cagagaatca
     3661 ttttttaagc atatcaagag cctatgtaaa aagaagagaa ttagtctctt ttcttgaaca
     3721 gcatcaaata aagttaaaga aaaacatatt agttggggaa gaaataaaca tacggaacgt
     3781 attatttggg gtatatttcg gggtctttaa tggtttttct atgccattta gagaaagcgt
     3841 catgaatgaa gcaaagagta tcgtgcaata ttttgaatat ctgttcaatt taaaactgtc
     3901 tgcaacaaaa cgaatcaaat tcaatttgct aaccactatt agtttgttga gggcctatgg
     3961 gaatcaacca atcggaaaac gtttcttttc agatgaatac tttaagagct cagaaggaat
     4021 gaagatcatc aaggatttat cacatatttc ttttcttcat atccctaaag agcagatcat
     4081 tgatgagatt tcttatatca tgttgttttt gatcacggaa gaagaatttg aattagatgg
     4141 cgcatttgga cttgcggaag ctcattttca tgatatccac atgattagtc aaaaaatgtc
     4201 agtggaattg attgatcaat taccattttc gattgaaatc tctgaagaag aaagactata
     4261 tatacgaaaa gaacttcgta tgggtatgat gcatgtcaat cgaaaaaatt ggatattcga
     4321 ctttgagatg actagcttta cgtctaaaaa gcagctacag tttttcttcg agacgtatcc
     4381 aatattcagt acagcaatcc aaaaggtcat acaagagaat aaacgctatt tcttgggaaa
     4441 agatgaacgt ttcatgattc gcttatttta tgattatctc tttttattgg tcggtatttg
     4501 tccggtatca tattttgaag caccgattca tgtctgtatt gatttttcgc aaggatctgc
     4561 ttataccgat tacattattt ctcaagtgaa aggatttaaa aattacaata ttgtgattga
     4621 gagtcgaatc acttctcgta cgaatattta tttatctgat tgtttacttg aaaaatttaa
     4681 taaagaacag ataatttgga aaaatccacc gactccagat gactgggaat attttggaaa
     4741 taccatcatc agagaaaaaa atagacattt aactgaaaaa atcagctagt atttatggat
     4801 ggcagctttg tctcacatat ttattagctg atttatgttt ttttcaaaga aaagg
//
LOCUS       DARBEC010000125         4829 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.132, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000125 DARBEC010000000
VERSION     DARBEC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.132"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..1118
                     /locus_tag="SCL80_002604"
     CDS             72..1118
                     /locus_tag="SCL80_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1108440.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIGSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            1135..2358
                     /locus_tag="SCL80_002605"
     CDS             1135..2358
                     /locus_tag="SCL80_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEG1108441.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            2447..3334
                     /locus_tag="SCL80_002606"
     CDS             2447..3334
                     /locus_tag="SCL80_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HEG1108442.1"
                     /translation="MKLGFIGTGVMGSAVARHLLEAGHEVAVYNRTKAKADPLVTEGA
                     IWADTPKAVAEQSNILFTMVGYPKDVEEIYYGQSGIFSADISGHILVDLTTSTPSLAE
                     KIAKTAKEKGADALDAPVSGGDLGAKNGTLTIMVGGEEAVYDQVLPLFKEFGTTFTLH
                     GSAGKGQHTKMANQIMIAGTMTGMTEMLVYAQKNGLDLKKVIETLSGGSAANWSLSNY
                     SPRILKEDYTPGFFVKHFIKDLKIALEEAEKMDLVLPATTQALKLYEELADKGFENDG
                     TQALIKLWWPEGKIPEKKS"
     gene            3420..3650
                     /locus_tag="SCL80_002607"
     CDS             3420..3650
                     /locus_tag="SCL80_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEG1108443.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(3707..4672)
                     /locus_tag="SCL80_002608"
     CDS             complement(3707..4672)
                     /locus_tag="SCL80_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEG1108444.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
BASE COUNT         1639 a          796 c          978 g         1416 t
ORIGIN      
        1 aaaaataggg cttaagtttg agtgacagtt tgtaggctat gttatactat ataagtgaag
       61 ggggacgtaa ggtgaaggta ttattatatt ttgaaagtga aaagatgctt gcaaaatctg
      121 gaatcgggcg tgcgcttgat catcaaaaac gtgctctgac ggaagtcggt atctcttata
      181 cactagatga aaaagaagat tatgacatcc tgcacatcaa tacatacgga atcaatagtc
      241 ataatatggt caataaagcc agaagagaag gaaaaaaagt tgtctatcat gcgcattcta
      301 cagaggaaga tttccgaaat tcctttatcg gatcgaacca gctttctcct atcgtaaaaa
      361 aatatctggt aggattgtac caaaaagcgg actatctgat cacgccaaca ccttattcaa
      421 aacagttgtt ggaaagctat ggtatccgtg tgccgattca agctatttcc aatgggatcg
      481 acttagagaa atatcaccct gatccaatga aagaacaaaa gttcagagaa tatttcaaac
      541 tgtctccgga tcaaaaagta atcatttgtg tcggtttgtt tttcgaacgt aaaggaatca
      601 ttgatttcgt agaaattgct aagaagatgc ccgaatacac atttatttgg tttggtcatg
      661 ttcccatgta ctcgattcct cggaacatcc ggaaaattgt aaaagaagat catcctgaaa
      721 atgtcctgtt tcctggttac attcgtggag agatcataga aggtgcgtat tcaggtgcag
      781 atctattctt tttcccttct tatgaagaga cagaagggat cgttgtttta gaagctttag
      841 caagcaaaca aaacgtactt gttcgagata ttccagtgta taatggctgg ttagaagata
      901 gcaagaactg ctatatgggg aagacaaacg aagaatttat ccgactgatc caacagatta
      961 ccaatcaaga actaccgagt acgacacaag ccggttatca aacagcaaaa gagcgaagta
     1021 tcaataaaat cggtgaagaa cttaaaaatg tatacgaaag tgtactgaat gaaaaagtct
     1081 ctagcaaatt aaaaaaagta aatcaggtca aggactagtt tggaggcgaa aaacatgaaa
     1141 atcggctttt ttaccgatac ctactttccg caagtcagcg gagttgctac atcgatcaaa
     1201 acattaaaac aagaattaga aagacatggg catgaagtct atatttttac aacgaccgat
     1261 cctaatgcgg ataagttgga aaaagatgtg attcgaatgc caagtgtacc atttatttct
     1321 ttcaaggatc gaagagttgt tgttagaggc atgtggtatg cttatttggt tgccaaagaa
     1381 ctagaactag atcttattca tacccataca gaattcggag cagggatact tgggaagatg
     1441 gtagctaaga agttgaaaat acctctgatc catacttacc atacgatgta tgaagactac
     1501 cttcattata tcggtaaagg aaaagtgatc aggcaatccc atgtaaaata cttgtctaga
     1561 ttatttgcaa atcatacgac aggtgtagtt tgtccaagtg aacgtgttat tgatacatta
     1621 cgcagctatg gtgtaatagc accgcttcgc gtgattccta ctggtatcga cattgaaaaa
     1681 ttcaaacgtt ctgatatcac tcagcaggat atcagcaaat taagggagtc actaggtctt
     1741 caggaaaacc atttgatgat tctttcttta agcaggattt cttatgagaa aaatatccaa
     1801 gccttgatta atgggtttcc tcaggtcatc tccgcttatc ctaacgccag attagttatc
     1861 gtaggaaaag gaccttatgt agaagagtta gaggaattga tctcagaact gcagttagaa
     1921 gaatttgtgc agttcacagg agaagtggac aacaatgatg tagcacttta ttataaagcg
     1981 gcagattatt ttgtcagtgc ttctacttca gaaacacaag ggcttactta tacagaagcg
     2041 atggcagccg gaacaccttg tgtcgtggaa ggaaatgctt atttgaaccg attgttcgat
     2101 catccttcat tagggaaaac ttttgaaacc gacgaagact ttgcaccagc acttattgat
     2161 tatatttcca gtgaagttcc taaagatcca aaagttttag aagctaaact ttatgatatt
     2221 tcatctacac atttcggcga agctatgatc gaattttatc aagacatggt tgtttatcat
     2281 gaagaagaga tgcgaagaaa agaagaagaa gactcgattg aacgaatcaa aataaaatta
     2341 aattcattta aaagataaaa ttcaaacatt ttttatgaaa aaatccttgt ttctagcgta
     2401 tactagcata gaagcaagga tttttgattt ttaggaggtc gaaaaaatga aacttggttt
     2461 tatcggaaca ggcgtcatgg gaagtgctgt cgcacgccat ctactagaag caggacacga
     2521 agtagctgtc tataatcgga caaaagcaaa agctgatcct ttagtaacag aaggagctat
     2581 ctgggcagat acaccaaaag cggtagcaga acaaagtaat attctcttta caatggtagg
     2641 ttaccccaaa gatgtagaag aaatctacta tggacagtcg gggatttttt ctgcagatat
     2701 ctcaggacat attttagttg atttgactac aagtacacca agcctggcag aaaaaattgc
     2761 aaaaacagca aaagaaaaag gcgcagatgc gttagatgca ccggtatccg gtggtgactt
     2821 aggagcaaag aatggtacac tgacgatcat ggtcggtgga gaagaagccg tttatgatca
     2881 agtactgcct ttattcaaag aatttggtac gacatttaca ttgcatggtt ctgctgggaa
     2941 aggtcagcat acaaaaatgg ctaatcagat catgattgca ggtacgatga caggaatgac
     3001 ggaaatgctt gtctatgcac aaaaaaacgg acttgattta aagaaagtga ttgagacgct
     3061 ttcaggtgga agtgcagcta actggtcttt aagcaattat tctcctcgta ttttaaaaga
     3121 agattacaca cctggttttt ttgtcaaaca ttttatcaaa gatttaaaga ttgcattaga
     3181 agaagcagaa aaaatggatc ttgtattgcc agcaactacc caagcattga aactatatga
     3241 agagttggca gataaaggtt ttgagaatga cggaacccaa gctttgatca aactttggtg
     3301 gcctgaagga aaaataccag aaaaaaaatc ataaaaaacg agttttttta gagaaaatca
     3361 atgcaagtag gctatgcttt tgatacaata taacagaaat aagaaaagga ggacctccga
     3421 tgacacattc acaattagtc gcaatcataa aacgactgga ggctatggtc gagtcagcag
     3481 ataatgaact gcaagttcgc cgttttgaaa aagaaggcgt agagaagtgt acagtaactt
     3541 atgataaagc gaccgataca tttgaattaa ctgaaacaga tactcatcag caatacgaat
     3601 ttgataatat cgatatcgta gcgatggaaa tctatgactt aatccaataa attttaggat
     3661 aattgacatt tgaagttgaa gatgcctaaa agaagccgag aagcaactaa tttagttgct
     3721 tttcagcttc ttttttgttt aacagttcaa tttcaaactg ttctttttct aattttttgc
     3781 ggtaactgat caagatttgt acaggatcga tttcttcttt tgacaattga cgtttcaaag
     3841 ataaatgagc ttctttcttt tcataataga agtcaggaga aacgtcacca gaggtaatca
     3901 ttgtttctgc aagttgacca taacaaacga ggtagtgctt tttctgactc tcatctgctg
     3961 ctaatggcat gctgaaggtg agcgatggtg ttcttaaaaa ctgcttttgt tcctggatcc
     4021 gtctaataaa ataagtaact aattcgatat ctttttgaag cataccggct ttttcgaaat
     4081 tcaactgata gatgaaatgt tgttgtcttt ttttcaagta agtcagtagc tttttatttc
     4141 ggccttgaaa cagcccttgg agttcctttt ttttctgttc aaaagtcatt tcctgtaggt
     4201 caggaagctg gactcgcaaa gctaacaaac taatttcaga caaccaaggc atttgataag
     4261 tttcttccat tatacgcaaa atagacggca tttttttata gagacgaaaa ggcccgtatg
     4321 ttcgagccgt aggagtatct gttaatacaa atcccttatt ggtaatatgt acataattat
     4381 aattactgaa ataattcatt tgtcggttat aaaaaggacg gtaatgttgg atcaattgac
     4441 actcgagcaa taaagcatct aattccgtgt ctactacgac gaaatcaaaa tctgttatat
     4501 gatgaatcat acgtaatact ttttttgaat gctgtttatt ctgatgaaaa tagctgctta
     4561 ctctattttt gagccgtttt gctttaccaa catagatgac cttaccatct cggtctttca
     4621 taagatagac acccggcagc atggggagag ttttcatttt ttcttttaac ataggatctc
     4681 cttttacgct ttgaataaaa agactataac aaattttgaa ataatggaca agttttaggg
     4741 aattctatag ttctgttcac agaaaatact ttgataatca aaatatgtga gatagatgat
     4801 ttttctaata aaagaaatta taaatgctg
//
LOCUS       DARBEC010000126         4788 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.133, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000126 DARBEC010000000
VERSION     DARBEC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.133"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            564..989
                     /locus_tag="SCL80_002609"
     CDS             564..989
                     /locus_tag="SCL80_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108445.1"
                     /translation="MICKNKKKIIAIIASFSLIFSTACTVLLTNNANVEAASSSYKHN
                     HAGYRCYKMSYYVSPSKCKQLARQTNKLNTVSAIANFIGLSGLTPGIISLVFGSAVSA
                     NNVFVTAARRGKGVQLNYIAHFSNTTTYNYSSNQNYVIK"
     gene            1019..1183
                     /locus_tag="SCL80_002610"
     CDS             1019..1183
                     /locus_tag="SCL80_002610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108446.1"
                     /translation="MKLTKIAVILAVIAGIILYFVLPANQFFLYFGIPAILIVNGVRI
                     FEQRKKCSST"
     gene            2141..3118
                     /locus_tag="SCL80_002611"
     CDS             2141..3118
                     /locus_tag="SCL80_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108447.1"
                     /translation="MANEIVKYHHELNTIPLRKFTSVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGIA
                     YFSKEDFHELLDIPKSYRESDINKKIIKPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNKALELVNQQIYSEKDPTSLQEKIKEQKRKLELL
                     QQLEELERKQNNLNKENKEINEQIPDNNSDIPPELKKGLLDNLQNLFKE"
     gene            3128..3625
                     /locus_tag="SCL80_002612"
     CDS             3128..3625
                     /locus_tag="SCL80_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108448.1"
                     /translation="MTDKTIKELADILGVSKDKVKYQLRKLPSNFTYKKGNIIYLKKE
                     AVTTITKVLSGNEPGNLPSELPDSLPTDYLLKEIETKNNHIEEKDRQIEKLQLALETQ
                     QKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENKSTTLSEETESIEKQSKTEKKW
                     WQFWQ"
     gene            4001..4282
                     /locus_tag="SCL80_002613"
     CDS             4001..4282
                     /locus_tag="SCL80_002613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108449.1"
                     /translation="MDILVRNINPKIITRIDKRCQELTTKTGKKWSRNQYLKILIEND
                     FDHELMNYKKDQFDLLFEKFIAIQAENTNILNDYVHTNNQLIETLIGGI"
     gene            4284..4700
                     /locus_tag="SCL80_002614"
     CDS             4284..4700
                     /locus_tag="SCL80_002614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108450.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDVNNYSEAIRFLCMSY
                     EPATNTTTTSNQAKLNAMGKEISIMTEIMAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     ENQLNPSRRNQPKNRQKETNQTFTSGNFDPTNFLKR"
BASE COUNT         1757 a          816 c          786 g         1429 t
ORIGIN      
        1 aaaaaaaaaa gaaaaatccg cttctgcgaa ttcttctttt ttcattttgg ggcgctgccc
       61 taatccctaa gtcccggtta ttcaatagca tggaaactca aagaataaga cgttccctgg
      121 aataaaaaaa aggctgctcg ttcgagcagt ccatacatag gaaaatgaaa aaacgacgtc
      181 ccttactgag aaacgctatt aaacttttaa caaatcctct aaaaaataac aagtctttaa
      241 aaagtgaccg gtaaaaatgc acaaagaact aaaaaagaga aattgcatca aatcaagcat
      301 tagaagctag taactagcta gaccaattga aaatcacaaa gtgagcgcct gtatctattg
      361 ctacaggcgc tacttggcat agtgtacatt atgtcaacaa gaatttaata taacatattt
      421 ttctagtttg ttataacaat gccactctaa caaaaatgta agcattatcc ttattaaaag
      481 ctccttgaca aggactatac cttttatttt atgattttta catagaaaac gttttctcta
      541 ttttttgttt agaggtgatt tttatgattt gcaagaacaa gaaaaaaatt attgctatta
      601 ttgctagttt ctctttgatt tttagtactg catgtactgt cttattaaca aataatgcta
      661 atgttgaagc tgcaagctct tcttacaaac ataatcatgc tggttataga tgttacaaaa
      721 tgagttatta tgtaagtcct tctaaatgca aacaattggc tcgccaaact aataaactta
      781 ataccgtctc agctattgca aactttattg gactatctgg attaacaccg gggattatat
      841 cattagtttt cggatcagca gttagtgcta ataatgtatt tgttacagcc gctagaagag
      901 gaaaaggcgt tcaattaaat tatattgccc attttagtaa taccactacc tataattata
      961 gttctaacca aaattatgtt ataaaataat taaaacttta aactaggagg aatgctttat
     1021 gaaattaaca aaaattgctg taattttagc tgttattgct ggcattattt tatattttgt
     1081 attgcctgct aatcaattct tcctttactt tggaatacct gcgattttaa tagtaaatgg
     1141 cgtaagaatc tttgaacaac gaaaaaaatg tagctctacc taatcgttac aaaacaacaa
     1201 tttcaccttc ggtgaaataa gcgaaacttt acaaaaagtg gctagcaaat gctagccttt
     1261 tttgtattgt agagcgtgaa ttttgagggg gattcaaggg ggagcttgcg cccctttgac
     1321 aagcggttat ttgcaccaaa tacctccgta tttggctagc aatccttcta aaaaatacac
     1381 ctatcgatca gagtcaaaac aaatcaattt tcgagtgagc gaatccgaat ttgcaaagct
     1441 gaagctttgc aaatgagtgt gccggcttcc gtaaaaatac atatggctat aatttgcgtt
     1501 ttaaggcgtt ttaaacgttc tggtatataa ttataccaaa acaactttaa aacgccttag
     1561 atctaatttt agaggtctta tagaacattt tagggttgtt agggcttgcc ctgaccgcct
     1621 gtaaggcgct ttgagaacaa gataagggtt ttatactaag actgtcttaa aacggcttaa
     1681 acaaacgctc agaacttttc aaaggagttc tttatgaact gtttttaagc aaaggagcct
     1741 ttaggcgact gcgcagtata atgtgagagc gtagcgatca cacattttta gtgaccgtag
     1801 ggagcgagtt tcccagttta gaaaaattcg cgaacttgtt tgaggaaaaa agcaggagtt
     1861 ttttaggcgg gaaattttgc ctcctctttt ataatctctt tttaaacctc tttttaaacc
     1921 tctttaccgt cggctgtatg cttactctcc caaggtgtgc agaatctttt gggggtcttt
     1981 ttgtctgttt ttaggggtct ttttgtctgt ttttaggggt ctttttgtct gtttttgggg
     2041 gtctttttgt ctgtttttag gggtctttaa ttaggggctt gacccctatt agaaagctct
     2101 ttataatttt gttaagtagt taatcttgaa ggaggatttt atggcaaatg aaattgtgaa
     2161 ataccaccat gaattaaata cgattccttt acgcaaattt acgtcagtag aaatgaattt
     2221 attcttctct atagtatcta gaatgcgtga cgtaggcgat aaaaaagtac aatttacatt
     2281 cgaacaatta aaagatttaa gcaattataa agcaactgca aatgttcgat tcatagatga
     2341 tttagaaaca acgtatgaca aattaatgga tttacgtttt ggtagaagaa gtgctgacgg
     2401 tttgcaacgt gaacgatttg ttttatttaa tcaatttaaa atcgatggaa aagctgatat
     2461 tccttttgct gaaatacaag tccatgagaa agccttaccc ttacttaata atcttgaaga
     2521 atgggttcga tattctctcc aacaattcaa tgaattagaa agcagttatt ccaaaaccat
     2581 gtttagatta ttaaaacaat acagaaccaa aggaattgcc tatttttcaa aagaagactt
     2641 tcatgaatta cttgatatcc ctaaaagtta tagagaaagt gatataaata aaaaaattat
     2701 aaagcctatt cgccaagaac taaccgcaat cttcaaaggt ctgacaatca aaaagaaata
     2761 cggaaaagga agaggaaaac ctgtcattgg ctatcaattc acgtttaaac ctgaaataaa
     2821 aaaagcagac gactttaata aacacgcacc aacaaaagaa gaacaaaata aagctttaga
     2881 actagttaat caacaaattt actctgaaaa agaccccact tcattacaag aaaaaatcaa
     2941 agaacagaaa cgaaaattag aacttttaca acaattagaa gagctagaaa gaaaacaaaa
     3001 taatctgaat aaagaaaata aggagattaa cgaacaaata ccagataaca attccgatat
     3061 tcccccagaa ttaaaaaaag gcttactaga taatcttcaa aacttattta aagaataagg
     3121 ggtttttatg acagataaaa ctatcaaaga attagcagat attctaggtg tttcaaaaga
     3181 taaagtgaag tatcaactga ggaaattacc tagtaatttt acctacaaaa aaggaaatat
     3241 tatctacctg aaaaaagaag cagtaacaac aattacaaag gttttatcag gtaatgagcc
     3301 gggtaactta cctagtgaat tacctgattc attacctacc gattacctac tcaaggagat
     3361 tgaaactaag aataatcata ttgaagaaaa agataggcaa attgaaaaac ttcaactagc
     3421 tttagaaaca caacaaaaat tactcgacca gcaacaacaa ctcaccctgc aagccaacca
     3481 acaaatcgag aaactgcagg aacaactaca gttgacttat acggaagaat cttcggaaaa
     3541 taaatcaacg actctttctg aagaaacaga atcgatagaa aaacaatcaa agacggagaa
     3601 aaaatggtgg caattctggc aataaaacca gcaatttaaa ttaataaaaa ttgctggttt
     3661 tattcttctt tttgcttttg cgagcagagc aacatgggcc catgttgcga tattttggtg
     3721 gcgccccaat ccccgttttt tcgttgaatc ttactcggac tgcactcgtt ttacagctga
     3781 tttttctgaa ttttgaaaga gcataaggaa acgtggtatc actttcttac gaaaggtgct
     3841 agataagctg tgcctatcac gtttctcaga aaaaaatttg ctatcacttt tttgccattg
     3901 tggctaggct atgtatagtg ttttggcgaa aaatagctat acaaatacta tatttgtatg
     3961 atatacataa attatatact gatacgaaag gaaaatttcc atggacattt tagttcgaaa
     4021 tatcaatccc aaaattatta caagaataga taaacgttgt caggaactaa caaccaaaac
     4081 gggcaaaaaa tggagtcgaa atcaatacct aaaaatttta atcgaaaacg attttgacca
     4141 cgaacttatg aattacaaaa aagatcaatt cgatttatta ttcgaaaaat ttattgccat
     4201 tcaagcagaa aatacgaaca tactaaatga ctatgttcac acaaacaacc aactcattga
     4261 aacacttatc ggaggaatat aacatgagaa aaacttctgt tcaaaaacac gtaacccttt
     4321 atcccagtca cgttgacgtt ttagattcca tcatcgaaaa aaatccagac gtcaacaatt
     4381 attcagaagc cattcgcttt ttatgtatga gttatgaacc tgctaccaat acaaccacaa
     4441 ccagtaacca agcaaaactc aatgccatgg gaaaagaaat ttctatcatg acagaaatca
     4501 tggcagaatt tgctgattta tatctaaaag atactggtgt tcttatcggt acaaattcta
     4561 aagtatatca agaagtgaaa gaaaaaattg aaaatcaact caatccttcc cgacgaaatc
     4621 aacccaaaaa tcgtcaaaaa gaaaccaatc aaacctttac ttctggaaat tttgacccaa
     4681 caaactttct aaaaagataa aaaaagaaac cgaaaaataa aaaaattcct cgatttcttt
     4741 ttagggatcg gtcttccagc aagatttgca agttggtacc aacttgcg
//
LOCUS       DARBEC010000127         4751 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.134, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000127 DARBEC010000000
VERSION     DARBEC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4751)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4751)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4751
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.134"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(182..1144)
                     /locus_tag="SCL80_002615"
     CDS             complement(182..1144)
                     /locus_tag="SCL80_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEG1108451.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1146..2585)
                     /locus_tag="SCL80_002616"
     CDS             complement(1146..2585)
                     /locus_tag="SCL80_002616"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEG1108452.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2770..4716
                     /locus_tag="SCL80_002617"
     CDS             2770..4716
                     /locus_tag="SCL80_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEG1108453.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKINGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
BASE COUNT         1509 a          812 c          785 g         1645 t
ORIGIN      
        1 acagttgtgt aaaaatatca ttttagagca tgaaaaaagg agtggatttc tccactccaa
       61 aaaagtctaa cttttggggg tcactacaag ttgacaactc ctttttgttt acaaatcaat
      121 tggatatcta caaattgtat tttctaacga attttataga cattaaaaaa ttaatttaac
      181 gttaaatact tcctccagaa agtattgaga ttggtaaaat ataatcctta tttgtctttt
      241 cgcctttaat ttttttcaga agaatatcaa caataagact tgcaatttca tcaattggtt
      301 gtcttattgt tgtcaattgt gggaaaaatt tctcaataaa agaggtacca tcgtatccaa
      361 taatttttaa atcttctggg atattaagtt taagttgatg agcaattttc atagtgagaa
      421 tggctgttaa atcatcagaa acaaaaatac catctggttt gtgatttata atagttgact
      481 ttatttccat ttcacgacgg atagtagaca gattattagg aattttgaaa atttttccat
      541 ttggattttg aaaagaaaaa cctagtgcac gtaattctgt gggagaatcc gtgttatcat
      601 ttcctgtaat cataatggta ttttgacaac catttttttg taaagtctta gctgcaagtt
      661 ttcccccttc aaagttatct gaagaaacaa tggaaacatt aggagctaaa tttctatcaa
      721 aagcaacaat aggtgctctc acacgctcat aatcagcaat tccaagatta tggcttgatg
      781 agattatgcc atcaacttgg ttggcttcaa gcatttcaat gtattctctc tctttagcag
      841 gatcattttg actattgcaa ataatcgttt tatatccatg tttaaataac tctatttcga
      901 gatactcaat gagttcagag taaaagatat tactaatatt aggaaaaata agaccaataa
      961 gctgaggtga ctttccttga agtccacgtg ctaaattatt tggtttataa ccaagagttt
     1021 tcatagcttc atgaactttt tgttttgttt tttctgataa atatccctta ttattaatga
     1081 cgcgtgaaac ggttgttggg ctaactccag ctaattcagc gacatcagtt aattttgcta
     1141 ccattttaat attttaattc aaagtatttt ccagtaggtt cgcctgattt gatttgaata
     1201 ccattttggt gattttcagg gaatacacga ctagtaaaca ctttttctcc tttattaata
     1261 aaaatctcaa tgattgagtt atctacaaaa atgttagcag ttgtttcttt agtatcaatt
     1321 gagcattcac gaatcgtacc aaagtcagtg gcatattgaa tgcctgcttt gctgcgatct
     1381 aaaataattt ttccttcttt ggtatctaca atcaagctaa gtccatttcc tttactatca
     1441 gagaaaagaa gtaactccga tttctgattt ggaggaaaag ttagttcaag ctcataagta
     1501 ttatttgttt ctgcttttgc tgtaaattgt tcagaactag cacgtaatga tgtaatagct
     1561 tctactggat attggtaaag tttaccatct tttattgaaa gttcttttac aagactcatt
     1621 gctccctgat aatcatactt atcagttgga taatcgatat caggtagtcc aatccaactg
     1681 acaatcagag cacgaccatc aggagtattg aatccttgag tcgcatacgc ttcaaatcca
     1741 taatcaagat tttgaatttc tgaagcgccc acaagtttta ctttttctat gtcaagcgat
     1801 tgacagatct taaaagtatt cggatagata ttcccataat ttagctcaga tttatccaac
     1861 ccttgaggac aataaatgag aacaggtttt ttgtctacaa atactagatt tggacactca
     1921 atcatgtatt ctgaaccagt gccgccaaaa tcaaggttgc caacttcctc ccatgtttcc
     1981 acgttattgt catccgcttt gtaaagtttg ataaagcctg atttttctaa attttgtgct
     2041 ccaacaatcg catacaattg tccttcataa ttaaacattt gtggatcacg aaaatgatca
     2101 gttacatcag caggtttatg aataagaaca ttctcacctt tcgtaatgtt gtagtcttta
     2161 tccatccatg ctccgatttg aagtgtatca cgttcccaat tttcatttcg aacgtttcct
     2221 gtatagagta agaatagatt cccctctatt tcatatgcag aaccggaata agcgccgtgg
     2281 ctatctcgtt ttgtgtctgg aagaagttct gctcctgtta catgaaaatg aactaaatca
     2341 tctgattcat tgtgtaccca ttctttcaac ccatgagcag ctccaaatgg ccaattttga
     2401 taaaaaagcg tatattttcc gttaaaaaaa gaaaaaccat taggatcgtt taacaatcct
     2461 gttttaggtt cgatatgata gctagcacgc caaggtgatc gttttacatt atccttaatt
     2521 ttatccatct catctttagt ccaatcagaa taagcacgat atcgtatttc tcgaggtaag
     2581 ttcatatttt actccttcaa tttatctttt tacaactagt atggtaaacg ttttccgttg
     2641 aaaaatcaat catttcattt aaaaaaataa actatatgac aaacgtttga catattttta
     2701 agatatgcta aaataaattt gcatttcagt gaaccgtttt cacgtaaaaa taaaaaagga
     2761 gtgattcgaa tgaattacag taaagtagct tccgacgtaa tacaagcggt cggaaaagat
     2821 aaccttattg cagctgcaca ctgcgctaca cgtcttcgtc tcgtactaaa agatgacacg
     2881 aaagtgaatc aaaaagcttt agacgaaaat ccagatgtga aaagcacatt taaaattaat
     2941 ggacaatatc aagtgattat cggtgcaggt gatgttaatt tcgtttatga cgaacttatc
     3001 aaaaaaactg gactttcgga aatttcaaca gatgatttaa aacaaattgc tagtaaagat
     3061 aaaaaattca accccattat ggcattaatt aaattattat cagatatttt tgttccaatt
     3121 atacctgccc ttgttgcagg tggtttatta atggcattac aaaacttttt aacagctgct
     3181 ggactttttg gaccaaaatc aattgaagaa atgtatcctg cgattgaagg tatttcctca
     3241 atgatccaac tgatgtccgc tgcaccattc atgttcttac caattttagt aggtatttca
     3301 gctgctaaac gttttggagc aaaccaattt cttggtgctg caattggtat gatcatgaca
     3361 acaccagatc ttggtggctc aactgaatat tggaatattt ttggttatca tgtttcacaa
     3421 acaaactatg cttatcaagt tattccagtt ttagcagctg tttggatatt atctatatta
     3481 gaaaaattct ttcataaaaa attgccatca tctattgact ttacattcac gccattatta
     3541 tcagtaatga ttactggatt tttgactttc acagttattg gccctgtaat gttattgttg
     3601 tctaatggaa ttacagacat tattgtttgg ctatataaca ctactggatt tattggaatg
     3661 ggtatttttg gaggaacata ttcacttata gtcatgactg gtttacatca atcatttcca
     3721 gctattgaaa ctcaattact ttcagcgtgg acaaatggta tcggacatgg agactttatc
     3781 tttgtagttg catctatggc aaacgttgct caaggtgctg caacatttgc catttggttc
     3841 ttaactaaaa attctaagac aaaaagtctt gcttcctctg cgggtgtctc agcacttctt
     3901 ggaattacag agcctgcctt gttcggagtt aacttaaaat accgctttcc attcttttgt
     3961 gctttaattg gttcaggaat tgctacagca gtagcaggat tacttaaagt agttgctgtt
     4021 tcacttggtt cagcaggttt cttgggattt ctttcaatta atgcaacatc tattccattt
     4081 tacgtattgt gtgaattgat tagctttgta gttgcttttg caattactta cttctacggt
     4141 aaaacaaaag ctgctggtat ctttaaagca gaagaaattg ctgaacaaac accagttaat
     4201 tcttcaaaag tagcaacaac agatccaata tctactgatg ttgaatcaac acaaacaaaa
     4261 gtttcatcag aaacaattgt tagtcccatt tctggtaaag tgattagtct ttcttcagta
     4321 aatgatccag tcttttcatc agaagctatg ggaaaaggga ttgccattaa accaaatgga
     4381 aacacggtcc attctccagt agatggtact attcaactca cgtttgaaac tggacatgct
     4441 tatggtatta aatcaaataa tggtgctgaa gttctaattc atattggtat tgatactgta
     4501 tcaatgggag gaaaaggatt tacacaaaaa gttactacta atcaaaaagt tagaaaaggc
     4561 gatattcttg gagcatttga ccgttcaatg attgagaaag aaggtcttga tgatacaaca
     4621 atgattattg tgaccaatac ttcggattat tcagaagtaa atatagtagc tgaaaataca
     4681 gtcactgagg gtactgattt acttgaaatc aaataaatat atttccctga gtaaagatgg
     4741 aattttcttt c
//
LOCUS       DARBEC010000128         4719 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.135, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000128 DARBEC010000000
VERSION     DARBEC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4719)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4719)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4719
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.135"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            91..807
                     /locus_tag="SCL80_002618"
     CDS             91..807
                     /locus_tag="SCL80_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1108454.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            826..2409
                     /locus_tag="SCL80_002619"
     CDS             826..2409
                     /locus_tag="SCL80_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEG1108455.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            2544..3122
                     /locus_tag="SCL80_002620"
     CDS             2544..3122
                     /locus_tag="SCL80_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108456.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            3068..3586
                     /locus_tag="SCL80_002621"
     CDS             3068..3586
                     /locus_tag="SCL80_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108457.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            3594..4634
                     /locus_tag="SCL80_002622"
     CDS             3594..4634
                     /locus_tag="SCL80_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEG1108458.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
BASE COUNT         1736 a          523 c          940 g         1520 t
ORIGIN      
        1 ttatagagga agaaacatta tttcaatgat taagagtcga gagcacaaac ggtagacgat
       61 gttattttta taaaaagata taatttatta atggtcgata attatgaaag gaaaaatatt
      121 cttaaaaaaa agagtatagg atgtcgtatg aaaatagtcg ctatagtacc aatgaagtta
      181 aataacaaaa gattgccaca aaaaaacacg aaagctttta ccaatggaaa accattgtgt
      241 tattatatac tttcaacact tttaaaagta aaacagattg ataaagtgta tgtctattgt
      301 agtaacccag atattaaaga atttatacca gaaggtgtaa catttttaaa gagatctagt
      361 tccttagatc aggatacaac gagtattaca caggtattag ccggtttcac aaaagaagta
      421 gaggctgatg tatatgtaat gacacacaca acagcaccat ttattagcga aaaatctatt
      481 gaaaaaggac tagatgccgt gctgacagga gaatatgatt ctgcatttgc tgcaaaaaaa
      541 ttgcaagatt tcttatggaa agacggtgaa ccatttaatt atgaattgga caatattcct
      601 agaacacagg atttgccgcc tttatacgaa gaaactagtg gattttatat ttataatcgc
      661 aatgtgatga ccgagttgca tagaagaatt gggaaaaaac cttgcatagt tgaagtaggt
      721 gagattgagt cagttgatat tgatgaagcc gaggatttta tgattgctga tgcgatttat
      781 aatcatctta ttttacaagg tagttaagac agggggaagg ttaggatgaa caatgtacag
      841 attttagatt gcacacttag agatgggggc tattgtaaca attgggaatt tggatttgat
      901 aatacaaaaa aaattatttc tggacttata gaatcaggaa ttgagattat agaatgtggg
      961 tttctcacaa ataaaacagt atacaataaa aatgttacaa aatatacgac cttagaagaa
     1021 ttagcacaga taattcctga aaaaagagaa ggaaaattat ttgtagcaat gatgaactat
     1081 ggcgaatata atatagagga tcttccagat tatgatggtt catctattga tggaattagg
     1141 attgcgtttc ataaaaaaaa ttgcaatgaa gcgttgaaat tatgtcgtga gataaagaaa
     1201 aaaggctact tagtttttgt tcaagctatg gtatctctca attatacaga tgaagagttt
     1261 ttagcagtaa taaaaaatgt gaatcagttt aaaccatacg ctttttatat tgttgatagt
     1321 tttggaatga tgaaaggaaa agatttaact cgtctttttt acatgattga gcataatcta
     1381 gaaaaagata tacagatagg ttttcactca cataataaca tgcagcttgc atattctaat
     1441 gcacaaaaac ttataaccgt acaaacaaat cgaagcctga ttattgattc ttcggtgtac
     1501 ggaatgggaa gaggagcagg taatcttaac acagaactat ttgtagaata tttgaatgat
     1561 aatgcaggaa aaaaatatca attaaaacca ttattatata taattgatga aatactaaat
     1621 agtttctatc agaagagtta ttggggatat tcattaccaa actatatttc agcagttcat
     1681 aatgctcatc ctaattatgc aagctatctg tcatataaga atactttaac aattgaagca
     1741 atgaatgaaa tattcgaaat gatggatgaa gaaaaaaagg tatcttatga taaacaatat
     1801 atagaagatt tatactacaa atatatggaa actgggaaaa tacaggagga atatatggaa
     1861 gaactgagaa aatttctctc agacaaaaaa attctactga ttgcaccagg aaaaagttct
     1921 gtagatgagg tagaaagaat aaaagagttt gttaccaaag aagaagtcgt aataattagt
     1981 gtaaattttg aatattctca tatagatata gattacgtat ttttaagtaa tttaagacgt
     2041 tttcatgaat taccgaaaga aagcagaact aaatgtattg ttacatcaaa tatacaagca
     2101 tcggatgtat atttacggac gagttataaa aagttgttat ctgaaaggga agttgtaaga
     2161 gacaatgcag gattaatggc aattagattt ttagccgata tgaatgtgga tgaaatattc
     2221 ttagctggat ttgatggata ttcacataat gaagatgaga attatggtga gcaatccatg
     2281 gaaattataa caaaatttgc attattagat gcaatgaacg tgcaaatgag agatgaattg
     2341 tcagaattag ctaaaaaagt aaaggtgaca tttttaacgg atccacgttg ggttcgaatt
     2401 gataagtagt agtaataatg tttttaaaat tttgataaag agatagtgag agaataaaaa
     2461 tgggtaaagt cttaaataaa aaagaagaaa aatatttaag ggacgatggg gcgagatgaa
     2521 actctaattt tgaactttta agaatcattt ctatattgtg tattgttgct taccatgcta
     2581 atttgcgttt aaattataat gcagacggaa ttatagtaca tggttcatta actgctaacc
     2641 agattttctt gaattatatt ggtatttggg gtcagctagg agttaattgt tttatattta
     2701 tttcggcata ttttttaagt gagaaaagaa acattcactt tgaaaatgtt ttgggaattg
     2761 tattagaatg tattttttat agtgtattag tttatggaat tctttgtgca tgtggaatag
     2821 aaacattttc attgaacagc tttgtaaaag aaatattatc atttgctcta ggtagatatt
     2881 ggtttattac atcctattta gcattgtata ttctaagtcc gtatttaaat atcatgctta
     2941 acaatctgga aaaacttcag ttgcaaaaaa taagtatgtt attgtttata ggtgtatgct
     3001 gttatcgtat gttatttaaa acatctttgt ttggggattg tgctgtttta attgcactat
     3061 atgtcatatg ttatacactg aaaaaaacga taataatatt tttgagagac acagaatcat
     3121 aggaacaatt gtatgtataa cagttatacc acttctcata tccataggaa tacttttcaa
     3181 atttatacca aatgcaatgg ttaaccagat ggttcatatt ggttctccat tgatggttat
     3241 agcaagtata tttttatttt atgtatttaa aaatttttca ttttacagta agtatataaa
     3301 taaacttgcg agaaccagtt taggggtata tattttacat gaaaatcatt atttcattga
     3361 tggtatgaag tctagaggct taatatggga tgggatattt ggattaggca aaaggttttt
     3421 agatagggat ttttggattt ggtttataat tgcagttgta gttgtgtgca taatttgcat
     3481 ggctatcgat gttgtaagag aaaaatttga aagacagatt tttaaaatta agagtgtaaa
     3541 gaacctgtgt aaacttatga atcatgttat aaatatatac ccataattaa taaatgagga
     3601 gaaaagaagt gaaagttata tcattcggag aattattatt gagacttgca gcacccggtt
     3661 attcaaagtt atttcaaaga gatagtttgg agactacatt ctgcggaggg gaagctaatg
     3721 tagcggtatc cttagcaata tttggatatc aatcagaatt tataacaaag cttccggatt
     3781 cagatgttgg atatgcggca gctaatgctt tgcgttattt tggagtaaat gttgataata
     3841 ttatatttgg agagggacgc atgggactgt attatttaga aaaaggagca gctcagagac
     3901 catctaaagt aatttatgac agaaaatttt ctgcaatttc gttagctgat agtgatgaat
     3961 ttaagtggaa ggagatccta aacgatgcaa attggtttca ttggacaggg ataaatccag
     4021 cattgtctga gaatctcttt gagacgtcct tagatgcatg tcaggctgcc aaaaaaagag
     4081 gtataacaat ttcttgtgat ttaaattatc gatccaagct ttggacatca gaggaagcaa
     4141 aagaaaagat ggaacagata ctaccattgg ttgatatttg tatttgtaat gaagaagatg
     4201 cagaaaaagt gctaggaatt aaagctgagc atacaaatat taatagtgga gaattaaata
     4261 aagatggcta tgtgcaggtg gctagggaga ttcacaagaa gtttggatgt aggtatgtag
     4321 ctacaactct gcgtagaagt tattcggcaa gtagaaatgg ttggagtgca atgttatacg
     4381 aagcagaaaa agataaagcg tatttttcca cagaatacaa catacaaatt gttgatcgcg
     4441 ttggtggtgg agacagcttt tctgctggta taatatatgc gttatgtgaa aatatgtccg
     4501 gacaaaaggc tattgaattt gcgactgcat ctagttgttt aaaacatact atagaaggtg
     4561 attttaatcg aactacaatt gaggatgtat ataatttaat gaatagcggt ggaaatggtc
     4621 gcgtgttgcg ttagtcaaca tataaagata aatcacagta atttttatgg tgattatctg
     4681 tcttagtagt gattagaata acgtgaaaat aaattgttg
//
LOCUS       DARBEC010000129         4713 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.136, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000129 DARBEC010000000
VERSION     DARBEC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4713)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4713)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4713
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.136"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(213..443)
                     /locus_tag="SCL80_002623"
     CDS             complement(213..443)
                     /locus_tag="SCL80_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="resolvase"
                     /protein_id="HEG1108459.1"
                     /translation="MDEAMDHPLLNKFIKDLILQIFSMIDEQEPTESKRHRAQGIIKI
                     AKRMFSIKGVLSYTVLIQKIPNHLVYKNNVED"
     gene            complement(673..1491)
                     /locus_tag="SCL80_002624"
     CDS             complement(673..1491)
                     /locus_tag="SCL80_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEG1108460.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1652..2341
                     /locus_tag="SCL80_002625"
     CDS             1652..2341
                     /locus_tag="SCL80_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEG1108461.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2355..3857
                     /locus_tag="SCL80_002626"
     CDS             2355..3857
                     /locus_tag="SCL80_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1108462.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3870..4346
                     /locus_tag="SCL80_002627"
     CDS             3870..4346
                     /locus_tag="SCL80_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEG1108463.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
     gene            complement(4443..4553)
                     /locus_tag="SCL80_002628"
                     /pseudo
     CDS             complement(4443..4553)
                     /locus_tag="SCL80_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252604.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1590 a          673 c          830 g         1620 t
ORIGIN      
        1 tatatttact tcagatggta aggcaacaaa ataaaataca ataggcagat gaactaataa
       61 ataaaatctg tattaaatac agataaatta atataaaaat caaaaggatc ataaactttt
      121 aacaagtgat gaaatgggtg gaaattgatt cttatagaca gtttgtctca taacattgaa
      181 gtattttgca attttagcat tgaaacactt ttttaatctt caacgttgtt cttataaaca
      241 agatgattgg gaatcttttg aattaacact gtatagctta gaacaccctt tatagaaaac
      301 attcgcttag caatcttaat aattccttgt gcccgatgtc gtttagattc agtaggttcc
      361 tgctcatcga tcatggaaaa aatttgaagg atcagatcct taataaattt atttaagaga
      421 ggatgatcca ttgcttcatc catactcgat aaactagtga taatgagcta cacgtttttc
      481 tttcaagttg atcaatagct tccacgataa aaatatcttt agataaaatc aagaaccgta
      541 ggaactacag ggatatttgg tcaataagag ccaccgctat tagtaaaaaa taagctatca
      601 agtatgctct catcccaaga attgcctact tcaatcgtta aaaaccgtta ggtttagcta
      661 agtgggggta gttcactcaa tacttttatc aataatagag cgcaaaactt tttctcttaa
      721 ttcaactgaa tcaatattat tgttttgttc ataaccatga actaatagat cacaaatata
      781 aagttgtgat atttttccag ctaaagatcc accattaaga aattcttcaa tggctgtttg
      841 taaaactaag tctgcagact ttcctatagg tgaatgaata tagttagtta tagcaataat
      901 ttttgcgcca ttttttttag ctattttcaa tgaatcataa gtatctttcg ttttcccaga
      961 taaagaaaaa atgattacta aatcattaac tgttaataaa gaagcgactt gggcttgaaa
     1021 atgagggtct agtacagctt ttgcttgaac tcctacgcga agaaacatat tttctaaatc
     1081 aagactagta tttccactag atcccactcc aaatatatat atgttttttg attgagtgat
     1141 cattttaatg gcttcattca atttttcttc gtctaaaagc ctaatagtgg aatagagagc
     1201 ttctgttaaa ctattagcaa tttcatcata gtactttaca ctagtcggat gttctttttt
     1261 ttgactaaag tcttcctttg caatttcaat ttttaaatca gaaaaaccag aaaaaccaat
     1321 tttttgacaa aagcgaataa tagtagcatc gccaactttt gtgtttttct taatatcact
     1381 catcgtacta taaataatag ttttcccaga atttaaaata aaatcagcga cttttttttc
     1441 agacttcgtt aaaaatggat aattttcttt aattaaatag atataactca tacaaactcc
     1501 tctacaattt caacttttct ccattatact ccaaaataat aaaaaaaact cctaaatact
     1561 ccatttttat ctttactttt atgaaagcgg atgctatatt gtttttggag ttgttatcca
     1621 aaaataataa aaaattggag tgtttaggat aatggataaa aagaggtttt tagatcttat
     1681 ttcaaaaggt ttgattgttt cctgtcaagc attacctggt gaaccatttt acaaagagga
     1741 agaaagttta atgccttatt ttgctttggc tgctcaaagg gctggagcag ttggtatacg
     1801 tgcaaattct gttcgagata ttattgaaat tagaaagtgt gtatcattac caatgattgg
     1861 aattattaaa agagattatc caccagaatc tccattcatt actgccacta tgagagaagt
     1921 agatgaatta gtagaaaccg gtgttgaggt aatcgctctt gattgcactc ttagaaatcg
     1981 acatgatgga ctatctataa attcttttat tactaaaata aaagaaaaat atcctcatca
     2041 gttatttatg gcagatattt ctacatttga agaaggcaaa aatgcatata aagcaggaat
     2101 agattttgta ggaactactt taagtggata tactgaggaa agtaaaaaac aagatgggcc
     2161 agatatagat ttgattaaaa aattggtaga agaagggatt tgtgtaattg ctgagggaag
     2221 gattcatact ccagaacaag caaaagagat aaaaaaaata ggagttgcag gtattgttgt
     2281 gggtggtgct attactcgac cacaggaaat tgcatcaaga tttgttaaag cattaagtta
     2341 gggggagaaa taaaatgttt aaaaaatttt cacaattagg acgcgctttc atgttaccaa
     2401 ttgctatatt accagtagcc ggattactat tgggactggg aggagcgtta acaaatgaga
     2461 gtgctataaa tgcttatcca tttttagatc aaccgtggtt gcatacaatt ttaagtatta
     2521 tgagttatgc tggaaatgca gtttttgcaa acctagcact aatttttgca attggtattg
     2581 ctgtaggact tgcaaatgga gataaaggaa cagctggttt agctggtgga gtagcttatc
     2641 tagtatatac tgcaactatt agtgggtttc ttgaactttt ttcagcaaaa gatgcaacac
     2701 tagatacagg tgtagtggga tcaattgttg ttggtggtac tgttgcattt ttacacaatc
     2761 gttatagaaa aattgaatta ccacaattct taggtttttt tggaggatca agatttattc
     2821 cgattgtttc tagtttagct gcaatcatta ttggttcttt cttttattta atttggccac
     2881 caattcaaaa tggtttagta gttgctggtg aacaaattgc ccaaatgggt agtttaggaa
     2941 cttttttata tggtttttta ttgcgtttaa caggtgctgt tggactacac cacaccattt
     3001 accctttgtt ctggtataca tctttaggtg gtaccgaaac tgtagctgga tcaacaattg
     3061 ctggagccca aaatattttc tttgcccaac tagctgatcc aaaccacact ggtctgttta
     3121 cttatggtac tagatttttt gcaggaagat ttgcaacaat gatgtttgga ttaccagcag
     3181 catgttatgc aatgtatcgt gcaattccta agaaaaatcg taaaaagaat ggtgggcttt
     3241 attttagcgg ggcccttaca tcatttttaa ctggaattac tgaaccagtt gaatatatgt
     3301 ttttatttgt tgctccttgg ctttatgtga ttcatgcatt cttagatgga ttatcttttt
     3361 attttgctga tatcttaaat attcgtgtag ggaattcctt ttcaggtggt ttgatcgact
     3421 ttttattatt cggtgtactt caagggaatg acaaaactaa ttggttaaaa ttaattcctt
     3481 ttggtattgc atgggcattt atatatttct ttgtttttac attctttatc aaaaaattta
     3541 aggtagctat tcctggaatg gaagaagaat cagaagttca ttctgaaaaa gtaataaaca
     3601 aaaatacatc attacatgaa gagtctctta tcattattga agctcttggt ggaaaagaaa
     3661 atattgagtc tgttgaagct tgtgcaactc gtttgcgtgt tgctgttacg aatggggaga
     3721 ttattcaaaa acaaacaata aaagacactg gtgcaactgc tgtcttcgaa gtgaagggtg
     3781 gcatccaagc tgtttttgga gggaaagctg acttattaag tcaagaaatc aatcaaatat
     3841 taggaaaaga taattaggag gtttttaata tgtttgaaat tttcaaaaag aaaaaacaag
     3901 aaacttttta tgcaccttgt tccggaaaaa tcatttccat cacagaagta aatgatcctg
     3961 tcttttcaca aaaattacta ggtgatggtt ttgccgtcat acctgatggt tcaaaaattt
     4021 atgctccatt ggctggtcga gtaacaaata tatttcctac taaacatgct attaatataa
     4081 aaagcaactc aggcattgat tatttgattc atattggaat tgatacagtt gaattacaag
     4141 gagagccttt ttcgatttgt gttcaagaaa atgatgaggt tactcctgaa acctcgttgg
     4201 ttgatgtgga ttttgacaaa ataataaaat ctggtaaaga tacttctgtt atagttgttt
     4261 ttccggagaa aaatcagttc aatgatttaa taatagataa tggtcgaatt gctcatggga
     4321 attcatgtgg aaaaatcact ttttaaaaaa gtgtatataa aaagctataa aataagctaa
     4381 ataggatatt tgtcaaatga agttggtgaa cagaaagaac tgttttcttt ctgttcattt
     4441 ttttatttta ttagtaatga ttccccatgc caaatcatga tccgtaattt aaagttataa
     4501 aagcttcgaa atccattggc atttcgttgg aataccttga tatgattatt caaatataaa
     4561 atatagagag aagagagaga gggaagatat ttataaaggg caaagttatg tttactcaac
     4621 ttaggaaata gaaatagatc aatttatatt ctcttgtcaa tcgaatatga accttagcac
     4681 tatataccaa atatagtaaa atgctgaaag atg
//
LOCUS       DARBEC010000130         4646 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.137, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000130 DARBEC010000000
VERSION     DARBEC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4646
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.137"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(133..717)
                     /locus_tag="SCL80_002629"
     CDS             complement(133..717)
                     /locus_tag="SCL80_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108464.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(928..1131)
                     /locus_tag="SCL80_002630"
     CDS             complement(928..1131)
                     /locus_tag="SCL80_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108465.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(1141..1563)
                     /locus_tag="SCL80_002631"
     CDS             complement(1141..1563)
                     /locus_tag="SCL80_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108466.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRDGIAIGNPTNDLTVTENYLIVSPLLALAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            1801..2415
                     /locus_tag="SCL80_002632"
     CDS             1801..2415
                     /locus_tag="SCL80_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108467.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIIASVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
     gene            complement(2580..3404)
                     /locus_tag="SCL80_002633"
     CDS             complement(2580..3404)
                     /locus_tag="SCL80_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001809248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108468.1"
                     /translation="MIERLKDFMISVWDVAMLADESNYTSSIYRLEDSNWIEISLELK
                     TLPNSVNYIIRDITSEAQQLVNYMHGHESIPFSLILLKRSKNISNTRDRFISIITACE
                     IGVKEFYQQEKPDLTLIFENLQSPPVDKLLGSMFKGYFNIEFPKEIRKEIKNFIEKRN
                     KLVHSPQGSKPTIEECFECYMAVLKTFNFFYKINDSFMYRDFYEESIELTPTEPNKSQ
                     ITLSKELERKVESQELAIGISITNVSTYKELYEAKSQDNKDFVENIDKEPTNTVGD"
     gene            complement(3428..3583)
                     /locus_tag="SCL80_002634"
     CDS             complement(3428..3583)
                     /locus_tag="SCL80_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108469.1"
                     /translation="MEYKFNLNLKEVLPTQGTININGQDNDDDIVIKKIEFIDSDINV
                     FFILKKN"
     gene            complement(4042..4299)
                     /locus_tag="SCL80_002635"
     CDS             complement(4042..4299)
                     /locus_tag="SCL80_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEG1108470.1"
                     /translation="MIKAWSDDAWDDYLYWHEQGNKSNIKKINKLIKDIDRSPFAGLG
                     KPEPLKHDLSGKWSRRITDEHRLIYRVENETIFIYSAKDHY"
     gene            complement(4292..4561)
                     /locus_tag="SCL80_002636"
     CDS             complement(4292..4561)
                     /locus_tag="SCL80_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEG1108471.1"
                     /translation="MEAVAYSNFRQNLRSYMKQVNEDAETLIVTSKDVEDTVVVLSKR
                     DYDSMQETLRTLSNNYVMEKIRRGDEQFSKGAFKTHDLIEVESDD"
BASE COUNT         1533 a          816 c          626 g         1671 t
ORIGIN      
        1 taatattaac aaatgaataa aatccttaca acatttgaga ataagttatc agtagacact
       61 tacaaaaaat tctcatacta ttgtcacttt tctagataaa tggcaaaaat tttcagcatt
      121 aaatgacaaa aactaatgag aatttgtctt tccatttttc tcctccattt ttgccaaata
      181 acgataaatg gtggttcttg agacgttcca ccgttccgca atagtcttaa taggtgtacc
      241 gctatccact aaagtcttca gtaattccaa atcttgttca gacaattttt caggacgacc
      301 accaaaccgt cctcttgcac gagcagctgc tcgtcctgct gcggatcgtt ccaggatcaa
      361 attacgctca aattctgcaa atgccgcaaa caaatgaaac atgagctgtc ctgtagaact
      421 tgatttgtcc atcgtaatat tttcttgaat actatgaaaa ctcacaccac gctcattcag
      481 tcgattgacg attgaaatca aatcttccat atttctacct aaacgatcta agcgccaaac
      541 gacaagtgta tcgccagagc gaacaaaatc aatggccatt tctaatcctg gtcgattact
      601 ttttgcccca ctcatatgat cagtaaaaat tttttcacaa ccaaatttat ttaggctgtc
      661 ttcttgtaaa tccaaatttt gtaaaccagt agatacccgg gcgtatccta ttttcaaaaa
      721 aatcactcct tcattaattt tattgtaaca taactttatc catatagttt aactgaacat
      781 agatattgtt atgggtttat gaacatgaaa tgaattgttt ttttgaaaaa taaagcaaaa
      841 aaagaaagtg tccagaaatg ggtgatttta tgaacacttt caattagaaa aaaagaatgt
      901 cgactagaca ctcttttttt aaaatcatta tttttctttt tgatatgttt tttggtctct
      961 tttttttctt ttttcttcag aatcttctag attagaaatt gatttttgaa ttttttgtgt
     1021 ctcctgtaaa gttcgctgaa cttgtaaagc tgattcttct ttcggtaaat taattttact
     1081 tagaatatcg ctatcttgat aaaaatttgg tgtgttcaca aattctggca taaaaaatcc
     1141 ctactttcta gttaaggttc ctgaataagt cgcaatccaa ttgtttccag ctgatttata
     1201 actttgtaaa tacaattttc catagtacca ataaccattt cttttttctt tccaattccg
     1261 tgattgagga ggtctagaag gccaaccaac tttatcaaaa atcatcgttc ctgtcactcg
     1321 tatcgtttta gaagctgcta gagcaagtaa tggagaaact attaaataat tttcagtaac
     1381 agttaagtcg ttagttgggt taccaatggc tattccgtcc ctatctaaag gagttaattc
     1441 ttggttatta tctactacag tagttggttc atttgtacta tcagcaaatg taggaattgt
     1501 agttccagtt acactaattc caatagttaa aatacctaaa gataaaataa acttattttt
     1561 cattgtctat cacctcgaaa tcaaaactaa gaaaacgttt tcttaataaa tcttaacaga
     1621 taaatcaagt tatatactag aaaattcata aaatatattt ttaatgtcat aatcgtttaa
     1681 attttcttga ttgacattta tttacaaaaa atattgccat ttaaaaagaa aagtgacata
     1741 tggcttcctc gtgtatgatt agggtgtatt ttaaatagtt gcagaaagta ggtaaaagtt
     1801 ttgagagttg catacatacg tgtctcatcg atcgatcaaa atgaacaacg ccagattgaa
     1861 gaaatgaaaa aattcggtgc agagagaatt ttcatcgaga aacaatctgg cgcaacgatt
     1921 actcatcgac ctgttttcca agaagcatta gattacgtcc gagatcaaga tatttttatt
     1981 gtggaagcta ttgatcgact tggaagaaat tatgacgaga tcatcgcttc agtcaattac
     2041 ttgaagaaga aaaatgtgaa gctgatcatt actagtttac cgatcatggc tgaagcaatt
     2101 ggcaatcctc tcctggataa attcattaag gatctgatca tccaaatttt ggccatgatc
     2161 gctgaacaag aacggactga atcgaaacgt cgccaggcac aaggaattaa gatcgcaaaa
     2221 gcaaacggcg tctacaaagg gcggcccaaa ttatacagtg cagacgcaaa agatccccaa
     2281 cgtcgccttg tttacaaaag catcgttgaa gatcttaaaa atggtgttgc gattgctaag
     2341 attgctaaag actacaatgt tacgagacaa accgtgtata gaataaaaaa agacagcatg
     2401 gtcaatgaca aatagttgat catgctgctt ttattatctt ctcatcttta atttcctaca
     2461 tataattact atctataaaa ttaagtctta ctcagaacag ataaagcata aaattccaac
     2521 aaaccatttt ttacttaact aaataaagag tctgttaata taatcttcac ttatttacgt
     2581 taatccccta ctgtatttgt tggttcctta tctatatttt ctacaaaatc cttattatct
     2641 tgtgatttag cttcgtataa ttctttgtat gtcgacacat tggttatact aattcctata
     2701 gctagttctt gagactctac ttttctctct agctcctttg acaaagtgat ttgagattta
     2761 tttggttccg ttggagtcaa ttctatagat tcttcataaa aatctctata cataaagcta
     2821 tcattaattt tataaaaaaa attaaatgtt tttagcactg ccatataaca ttcaaaacat
     2881 tcttctatag ttggtttact tccttgagga gaatgaacca atttattacg tttttctatg
     2941 aaatttttaa tttcctttct tatttctttc ggaaactcaa tattaaagta ccctttaaac
     3001 atgctcccta ataatttgtc tacaggggga gattgtaaat tctcaaatat tagagttaaa
     3061 tcaggtttct cttgttgata aaattccttt acccctattt cacaggctgt aattatcgaa
     3121 ataaacctat ctctagtatt tgatatattt ttacttcttt taaggagtat aagagaaaag
     3181 gggatacttt cgtgtccatg catgtaatta actaattgtt gagcttcaga agttatatct
     3241 ctaattatat agttaacgga attaggaagt gtctttaact ctaatgaaat ttctatccaa
     3301 ttactatctt ctaatctgta tatgctcgaa gtataatttg attcgtcagc aagcatcgct
     3361 acatcccaaa cactgatcat aaaatctttt aaacgttcaa tcatttcagc aatattgcta
     3421 aaatcagcta atttttcttt agaataaaaa aaacatttat atcagaatca ataaattcta
     3481 ttttttttat tactatatcg tcatcattat cttgaccatt tatgtttatt gttccctgag
     3541 tggggagtac ttctttcaaa ttcaagttaa atttatattc cattacttct cctccaatat
     3601 ataaaaatta cttaactgta tccaaattta atatgtaaat aaatcattaa tttttagcta
     3661 aattctatga gaagtttatt tttgttgttt ttgctaatta actaaaacta gtacaaaaaa
     3721 tttaaaatag attattaaac ctattgctta ttctattcag tcatatatat aagcgtcatc
     3781 ctttagtaac taacatccca cactacaata gtattataac atatctattc aacttcaaat
     3841 tgttgaaatt taagccattt atatttactt ttcctcaact ttagaaatga ttttactaac
     3901 cttatatccg taatacgcgt aacaaacaca ttcattaata acaatcaaca ctaagctata
     3961 ttagcttttt aaatgactag ggtaaataaa ataaggatca aaaactttta agtttctgac
     4021 cctttcctta cttccgattg gttaatagtg atcttttgca gaataaataa atatcgtttc
     4081 attttcaact ctatatatca gtctatgttc atctgtaatt cttctggacc attttccaga
     4141 taaatcatgc tttaatggct caggttttcc taatccagca aagggggaac gatcgatatc
     4201 ttttattaac ttgttaatct tttttatatt gcttttgttt ccttgctcat gccaataaag
     4261 ataatcatcc caagcatcat cagaccaagc cttaatcatc agattcaacc tcgattaagt
     4321 catgtgtttt aaatgcacct ttggagaatt gttcatctcc tcgacgaatt ttttccatga
     4381 cgtaattatt agaaagtgtt ctcaacgttt cttgcataga atcataatct ctttttgata
     4441 atacaacaac tgtatcttct acatctttac ttgttacaat aagtgtttca gcatcctcat
     4501 taacttgttt catataacta cgtaaatttt ggcggaaatt tgaataagct actgcttcca
     4561 ttcctttcac cccttattta tttctataaa acaattgtac attatattgt acaattaagc
     4621 aaatgattaa tattctaact tcttat
//
LOCUS       DARBEC010000131         4634 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.138, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000131 DARBEC010000000
VERSION     DARBEC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.138"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            174..518
                     /locus_tag="SCL80_002637"
     CDS             174..518
                     /locus_tag="SCL80_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEG1108472.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="SCL80_002638"
     CDS             519..938
                     /locus_tag="SCL80_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEG1108473.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="SCL80_002639"
     CDS             complement(1030..1380)
                     /locus_tag="SCL80_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108474.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2566)
                     /locus_tag="SCL80_002640"
     CDS             complement(1346..2566)
                     /locus_tag="SCL80_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEG1108475.1"
                     /translation="MLSNTPTDNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILT
                     LLSVLLAYRHKVILNGPLFASILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQ
                     YIMIGLFGSALSPVVSYITFGMRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGF
                     TLYNIGFTSGLIAMTFTAVLRLFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFY
                     YNSFRLLGIREIFDSSGKLTTDFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPV
                     IGAILSAVGFSAFGCHLKNSFPILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPIS
                     GFYGSFYGVIAGVLHIALVHNVSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKE
                     KKTLGKRIIKKNHR"
     gene            2932..3498
                     /locus_tag="SCL80_002641"
     CDS             2932..3498
                     /locus_tag="SCL80_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEG1108476.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3643..3730
                     /locus_tag="SCL80_002642"
     tRNA            3643..3730
                     /locus_tag="SCL80_002642"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3675..3677,aa:Ser,seq:cga)
     gene            4064..>4634
                     /locus_tag="SCL80_002643"
     CDS             4064..>4634
                     /locus_tag="SCL80_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEG1108477.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1684 a          778 c          891 g         1281 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaacacct gtaaaaccaa gataaaaata ctttcttttc tgtgaaacga ttcgtttttg
     2641 aggatgaata taaataacgt cagtttcgtt ttccattaca caccttcttt ttcattttct
     2701 ttaactataa cttgaaatga aagatttgga caactatcat ttaggtcaaa actaaaaatg
     2761 taagggaacc cattgaattt aaccaaattt ttttaatttt gcaaaaaaaa gttaaagaag
     2821 actgaaaaaa aatttaaaat ataacgtttt gagtaaaatg tttgcaaaaa atgctaaaaa
     2881 tagggtacaa taaaacaaca tatactgcag taaagaagtg ggggagtttt aatgagaaga
     2941 aaagtctata ccaaggatca aattctaaaa gctgcttatg atgttgttgc aaaggaaggg
     3001 tttaaaggat ttactgcgag aaatattgct aaaaagatgg ggatttcgac acagccgatt
     3061 tatctggaat tcaaaaacat ggaagattta aaaaatacgt tgctggattc tatctttgaa
     3121 caactaaaag aagatgtttt ccctattgaa cgtactggtg ataaaatcat tgatctcggt
     3181 ttgaactata tctactttgc aaaaaagcat catagtcttt acatggcatt gtacattgat
     3241 gaacatgggg acggaaagaa aatgcatgat ttttcataca attatttccg tgaaatagtt
     3301 aaagaagatg aaagatacaa tcaattatcg gatgaatatg tcgatgctct gcatactggt
     3361 acatgggtag cagtgactgg cttagcgtca ttgatgtcat caaacatcat gcatccaaca
     3421 gaagaacaaa tcattgcact gatcaaacgg acaattgact cgattttagc attagataat
     3481 cctgaaataa tcagatagtg ttctatcagt atgaatttga tcggtaagtc cgttggctgc
     3541 aaactgtcaa tggacttttt tgctaaaaaa agcgatttct gttatttatc tattagaaat
     3601 aggcttgaca tttcgtgaaa aaagtttaaa aatagtactt acggaaggtt ggcagagtgg
     3661 taatgcaccg gactcgaaat ccggcgaacc ggctaatacc ggcgcgcagg ttcaaatcct
     3721 gtaccttcct tatatcaacg attagaggtt gtgaagaagc tgtcctgcat caagtaataa
     3781 gaacggccaa acaaaaatag cttttcctat tttttcgttt gattgggctt attaccgcag
     3841 atgcaagctt ttgaacaccg tttgttcgga ataaggggct gtgacaagac ttttgtcaca
     3901 gccccttatt gttgtacttt taacctacaa tattttttga ttccccctga gtactatttt
     3961 ttataaatgc tagtaaatca atcttctttg gccttttttt gataaaaaaa atactagatg
     4021 taagataaaa gtgtaaaaac gatttgtttt aggaggggaa acgatgtgta ccgcaatcac
     4081 ttatgcaacg aaagaccatt attttggaag gaattttgat tatgagatgt cttataatga
     4141 agtggtcact attactccaa gaaattatcg tttcgatttt agaaaggtaa aaaatttaga
     4201 taaacattat gcaatgatag gaatagcagc tggggtatcc aactatccac tctattatga
     4261 ggctacaaat gaaaaagggt tgagtatggc aggactgaat tttcctggca atgctgatta
     4321 taaagaatta caagaaggaa aagataacgt tgcaccattt gagtttattc catggatact
     4381 agggcaatgc tcaaccatag acgaagctaa agaattattg gctactatca atctagtcaa
     4441 tatcgatttt agtgaaaaac tacccttgtc acctttacac tggttattag ctgataaaga
     4501 aaaatcgata gtaattgaaa gtatgaaaga tggacttcat ttatatgata atcctgtcgg
     4561 tgtgttgaca aataatccgc catttgatta tcaattattt aatctgaaca attatcgctc
     4621 gctatcaaat ggaa
//
LOCUS       DARBEC010000132         4473 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.139, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000132 DARBEC010000000
VERSION     DARBEC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4473)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4473)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4473
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.139"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            321..1052
                     /locus_tag="SCL80_002644"
     CDS             321..1052
                     /locus_tag="SCL80_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEG1108478.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            1197..3224
                     /locus_tag="SCL80_002645"
     CDS             1197..3224
                     /locus_tag="SCL80_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEG1108479.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGHSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRIDIPVENQRIGTFEGKLSWILADVPGN"
     gene            3332..4429
                     /locus_tag="SCL80_002646"
     CDS             3332..4429
                     /locus_tag="SCL80_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEG1108480.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
BASE COUNT         1572 a          717 c          923 g         1261 t
ORIGIN      
        1 aaaatataat atacattttt tatttaatta taattaatat tgattgaagt ctaggcagtc
       61 aagtcacatt ttgatagata ggagggggac ttacgaatta ttttaaaggc tatgacagct
      121 gtatttctag attgtgtttg catcaagttg caataaaaac tagaaatcaa atcgggctga
      181 taatcattta taactaatcg atacatagga ccaaatatag gtctatgttc ttttatttca
      241 taagtatcgt aaaacagaat tttgctatgg attaatccaa tcgactgata tgtttagtaa
      301 ataaagaaag aggaatgtca atgatgaaaa aagaaatcgt attaacagca gcaatgatgt
      361 tattcagcat ggtagcaagt acgacttttg tttctgcaac tgaagtatac ccaaaggaat
      421 ataacacaga aggaacgatt acatttgaag caggagatga gggtgtaact cctcctgttg
      481 atccagagaa tcctgatccc aataaacctg tcgatccatc agatcctcca agccctggaa
      541 caggaggggc attatcgatt gattatggat caaaattcaa atttgggact caaaaaattt
      601 ctactgcgga taaaacgtat tatgcagctg ctgatgtgat gaacgatggg tctagaaaac
      661 cgacatatgt ccaagtgaca gataggagaa gtaccttatc tgggtggaaa ttaagtgtct
      721 ctcaaccaga acagtttaaa actgccagtg gggatgaact agttggagca cagctgaaat
      781 ttacgaaagg gcaggctgtt tctttggttg atccaacata tacgccgcaa acggtgaatt
      841 ctgaattaac gttgacaccg ggaggaaaca atacgttagc aattaatgct aaatcaggaa
      901 caggtgtggg gacttgggtc tatcgatttg gtgcaaatga aaatgaaaat caagatgcgg
      961 tacagctttc ggtacccggt aaatctgtca agttagcgca acaatattcc actaaattag
     1021 tttggacatt agaagataca cctaacaact agtttactga aaagaactgt taaaaataaa
     1081 tacgtagtca tattgtcttg cgaggtaatc gagcaaaaat tgtcaaaggc taggctaaat
     1141 ttgtttggat acgaatagtt catctaggaa tttaacgatc aggaggaaaa gatatattga
     1201 aaaagaggag gatcgtgctc attttaagca gtctcttagt gactaatatc tttccgatca
     1261 tagggtactc tttggaattg aatcagttgg agcatggaaa tagaagagac gaagaaagca
     1321 aactcacttc gacattcgac agtttaatag aatccaacca gacacaagaa agcagtgagg
     1381 atttaagtat ttcgagtagt ggagagacca ctgacaacgt agaagctgag ggttcggaca
     1441 aacaaggact agaagatgca ccaatttctg cggttgagct gcctgacgat cccaatatta
     1501 tttctatcga taaaattttt caaacaccta tcggtactag cacgtccata ttggaaaatg
     1561 ggaagttact gcagttgaac ccagcagaaa ggtctcaaag aggagctatt tggtcaaata
     1621 agaagattag tttactctcg gattttacat ttaaatctta tttatactta ggaaatcaac
     1681 gagatagagc gggagatggc atgaccttta cattgactgg cgatcctaga atggctagta
     1741 atcccagtca agttattgga tcgccaggta tgggaattgg ctcatattct actcgttctg
     1801 gccaacctta cattgcgaat gcgctctcga ttgagtttga tacgtataaa aatagtggtt
     1861 cttctgatcg aatggatcga gagataactg ctgataatag agggcatgga catgtcgctt
     1921 ttgtcacgcc taaaccaaat aataataatt attctggaga gcatactggc gtgacggtag
     1981 ctcctacatt tctttctaat ggcacatggc gcttgttaat aattaaatgg gacgcggagc
     2041 aaagaagtct tacttatgat ttggaaggtg tcggtcactc ttctcggatt atacctgatc
     2101 tcattaggca gtttggttct acagaagtaa attgggggtt tacttcttca acaggtactt
     2161 tttttcaaga aaatgctgtc gctgtcacgc gacttccatc gaatgtcaca tcaacagctg
     2221 atgtaaaagt caacgatagt gattatgggg ccatcgctga agctggagga aacgataaga
     2281 taactatcag aaatacattg aaggtcgagg gggattttct tgaatataag aaaatgcaga
     2341 ctagtatcga cctcccacaa gaattaacta ttcccgagca tatcttgatc aatggaaatc
     2401 aaattgatgc caaagatatc ttgataaccg gaaatcgagt cacgctaaat ctgacagatt
     2461 attttattgc tggagatacg ttaataatcg aattggagac aactttgcag gaagtaattc
     2521 ctgaaaaagt actcactatg cattttgaat accttgaaaa cgggaatttg attgagcaat
     2581 cgaatgaaat aaacctaaag atcgcaaagc tgaaggagat agatatccat gtttattata
     2641 aagataaaga cagtcagatt gatattgctg aaatgaaaac aatcactggg aaaataggag
     2701 agaagtataa agaaactcct attgatattg aagggtatgt ttttgactcg gattcagcga
     2761 atacagaagg catcttctcc gaaaatacag aggatatcta tttttattac agaaaaggca
     2821 aactttatct ctcaactgct cctcaatatg tcaattttgg aaaacataaa atatccagta
     2881 gaccattaag tgaattcgga cagttttcag gtgggttgaa aattatagat gaacgggctt
     2941 ttggaagttg gaaattacaa ttgaaacaag agaaagttct tacaaatgga gattttgagc
     3001 ttccagaagc tctttccttt gttacctctg aaaatacaac ggttataggg acatccgctg
     3061 ttacgatttt cgaaagtgat caaaagggtg aaagcgattt gtcctctctt ttagattcta
     3121 cagctcatcg tggaatcaga atagatattc ctgtagaaaa ccaaagaatc ggaacctttg
     3181 aggggaaact atcttggata cttgcagatg tcccaggaaa ttaacaggaa gagaagaata
     3241 taacaaaatt attgagtttc aagaagggca caggtaccat aggctcttgt gtcccacaga
     3301 gacaagattt tgttatagtc ttctcttaca tatgattaaa ggagcgattt tcttgagacc
     3361 aaaacaacaa ttatttataa cactcctagt tattttaggg gcacttttta acaatatatc
     3421 agtgtcagct agtgcattta atttttcagt gacgccgata acttcagaga atcaaatcga
     3481 caagagaaaa acttacttcg atcttcaact tgatccggat caggaagtag aagtaaaagc
     3541 ggaattgaga aatgatacag aaaaagaagt gaaaatcgat atttctgtga atagcgcaac
     3601 caccaattca aatgtgatgg ttgaatacgg aaaaaacgag attgaaaaag acatgagcct
     3661 gatctttgat ctggtagatt atgtgagcta tccacagata gtgacgctaa aacctaaaag
     3721 tgtccaaacc gttgcttttc atgtcaggat gccgaatgag cgatttgatg gtgtattggc
     3781 tggtgggatc acattccaag aaatccagac ggaaaaggac caaacagaaa ctaaggatca
     3841 aagcttatcg attgagaatg aatacaccta tattattgct ttgttgatgc aacagacact
     3901 gaacgaagtt gctccgaacc ttactcttca tgaagttaaa ccagatcaaa tcaatgcaag
     3961 aaatgtcatc ttagcaaacg ttcaaaatga tcaaaaaacc tatatcaatc aagtagttat
     4021 tgaaacaaaa attacaaaaa aaggacattc tgaagtttta tatcaagagg aaaaagaagg
     4081 attgcagatt gcgccgaata caaatttttc ttttccaact gctttaaatg gtcaaccgct
     4141 aaccccaggt gaatatcacc ttacaatgac tgttttagga aatgaaaatg aaaatggaaa
     4201 attttcgaga aaaaaaggaa atactacgat caactatacc aatcaatggg tatttgaaaa
     4261 agattttaca atagacggaa aagtagccaa ggaattgaac acgacggatg ttacgattaa
     4321 aaaagacaat aattggattt atatcttagt agcttcactt ttcttattgt ttattttatt
     4381 atgcatactt ttgtttgttt tgagaaaaaa agaagagaaa gaaaagtaag gttaagataa
     4441 caaatagtaa atttttatta ttgtaggatg gtt
//
LOCUS       DARBEC010000133         4425 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.140, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000133 DARBEC010000000
VERSION     DARBEC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.140"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            690..1658
                     /locus_tag="SCL80_002647"
     CDS             690..1658
                     /locus_tag="SCL80_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEG1108481.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="SCL80_002648"
     CDS             1669..2484
                     /locus_tag="SCL80_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEG1108482.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="SCL80_002649"
     CDS             2552..3262
                     /locus_tag="SCL80_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEG1108483.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4425
                     /locus_tag="SCL80_002650"
     CDS             3298..>4425
                     /locus_tag="SCL80_002650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1108484.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIKN"
BASE COUNT         1490 a          661 c          921 g         1353 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaat
//
LOCUS       DARBEC010000134         4396 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.141, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000134 DARBEC010000000
VERSION     DARBEC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.141"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..93
                     /locus_tag="SCL80_002651"
     CDS             <1..93
                     /locus_tag="SCL80_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014125701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1108485.1"
                     /translation="RGLYKKTRKEGTLFGFSVCTEIKVLLGIPA"
     gene            complement(151..1371)
                     /locus_tag="SCL80_002652"
     CDS             complement(151..1371)
                     /locus_tag="SCL80_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEG1108486.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1391..2002)
                     /locus_tag="SCL80_002653"
     CDS             complement(1391..2002)
                     /locus_tag="SCL80_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEG1108487.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(2045..2977)
                     /locus_tag="SCL80_002654"
     CDS             complement(2045..2977)
                     /locus_tag="SCL80_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEG1108488.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3398..4258)
                     /locus_tag="SCL80_002655"
     CDS             complement(3398..4258)
                     /locus_tag="SCL80_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1108489.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1257 a          882 c          727 g         1530 t
ORIGIN      
        1 cgaggattat ataagaaaac ccgaaaagaa ggcactctct tcgggttttc ggtctgtact
       61 gaaatcaagg tattattggg aatcccagct taaatcatag ataccgtaag ggactttatt
      121 ctttatttaa aactttgcaa cagaaccttt ttattttaca gaagcactcg aaactgcatc
      181 tactgcagtt tcatcgtgtg tgctggcgct ggttactgca tctgtttttc caccatgaac
      241 agatgcactt gaaaccgcat cttcatttcc aacaagttgt tgacccgttt gttgtaaata
      301 ccactcaatg attggtttga aatcaagaat gtattcattg attccagcat actctccatt
      361 ttcatcatgc attgcttggt aattatgcac aatatatttt tctggcactt gttttggtac
      421 atgaacacgt accgcatctg ttgctccgct tcgcagttgt tgaattaccc actctacatt
      481 tttatacgat ttttctgggt gacagttggc taatggattc cctacctgac cgggcacacg
      541 agcagctaac atctctttcg cttcttggcg caaattataa tataaaaatt ggttattatt
      601 atctgcaaaa gttaattcca tcggcatcga atctaaaaag taattgattt gatccactgt
      661 caataaccca cgatttaatt tgacgtaagt atttccagaa acggcaccga ctgcttcaga
      721 agctttttcc aaccaatgat catctgacat atcaacatcg ggtattgtcg tagcaattgt
      781 tcctgtcgca tgcaaatctt ctggtggaag ctttttaggc cctgctaagg tagacacgac
      841 tttaatgaat tttacaaaac ttgctaatgt actgcctaaa aagtcaacag ttcgtggatc
      901 aatcaagcga tcttcttggt caaatgcttc ttttgctttt cccaataaaa attcatttcc
      961 gggcatcact aatgcattca cgcctggtgc gtccaaaatt tggcgcaagt gtaattgcgc
     1021 gcgtgaagtt ccttgatcgt agtaagaagc acccacaacc attacaggtt tattttcaag
     1081 tggatggatt ttaaatgata gccattcaag tgcacttttc aatcctgctg gaaccgtatg
     1141 attgtgttct ggcgtagcaa taattacccc atcagccatt aagattttgc gatttagctg
     1201 ttgaatagca ggactatttg tttgatcatc atcttgattg aacagaggta aatctttgat
     1261 ttcaatgatt tctaaattaa atagttcatg atattgtttt tggataaatg ttaataattt
     1321 acggttatat gaaagttcag ctgctgatcc gacgatacct attaatttca tctttgtttg
     1381 ctcccctcga ttaaatcgtt tcccatgaaa agctttcggc ttcacgttta tttgcttggt
     1441 gcgcatgttg caattgacta gtgattgtga caaagagtaa gaaatctttg aatagttcac
     1501 gtaattttgc gacttgttct tcatcaatga gtcgtccttg ctcatcaaag gcttggagtg
     1561 aatgacccaa taaaaattct gagcttggca taattcgtgc ctttaactct ggtgaatcca
     1621 atatttggcg caaatgtgct tgtgcgcgtg aagaacctaa cgtgccataa gacgctccgg
     1681 taatcatcac tggtttatcc acaaacggaa aaataccata tgaaagccaa tttaatgcgt
     1741 taatcaatga agcaggaacc gcatggtcat actctggtgt gctgatgatt acgccatctg
     1801 cttgttcaat ttttccagca atttctttaa caacatttgg tagttctgtg ttttcaggtt
     1861 tgttaaatag tggcagctcc ttgatttcga caatttcgat ttctgcctca tttacaaaat
     1921 aatttttcat aaactgtaat aattcacggt ttgttgaccg cgttgaattt gttcctacta
     1981 atccaataaa tttcatcttc attctctttc ctttctaaca tcgatttctg actgttcgca
     2041 cgtactaaat tttttcaaat ccttttgtat acaagaattg attctctttt gttacaactg
     2101 ctccctcgat acccggctgt tcttcaagcg ctttcaacac ttctaaagaa gatgctccaa
     2161 ataaacgtgt agtccagatt tctccatcta cagaagaatc agacaaaatc gtcaggcttg
     2221 ctacgtcatt tttaactgga aagccagttt tcggatcaat gatgtgatga tacactgacg
     2281 aaccattttg tagataacgt tcgtagattc cagaggtaac taaagattgg ttgtttagtt
     2341 taaccaccgc aagcgactct tcacgtgaac ctttaggatt tcgcaaacca atacgccaag
     2401 caccatccga acgtcttggt gattcaccaa aaacaacgat atttcctcct aagttaacta
     2461 aagcaaacgc tgcatttcgc tctcggcaat gtgtaattaa tttatctgca atgtaacctt
     2521 ttgctaacgc ccccaagtca atcatcattc ctggtttggt taaaaaaacg gttttcattt
     2581 tttcatctaa ttgaatttgg tgtggatccg ttagcagaag taactcagtt atctctgctt
     2641 ttgttggaac caatgcatct tcaaatccaa tgtgccattt ttgtacaacc ggaccaatag
     2701 caatattcag ataactattt cttgcacaac tatgtgcttt tcctaatttg atgagctcaa
     2761 aaagttcagg atgtacaatc acagcttgtc gtccagcagc ttgattgatg gccattaatt
     2821 cagaagttgc atcatgtgcg ctgaagcgat gttcataaac gtgcaaccat tcgcctaact
     2881 ctgctagtaa tcgactaggc tcttcatgcc caacttgtaa tgtaattgtt gtccccatca
     2941 tttgccagat tttcgtttct tcgatcacct gattcatttt gtaccttctt tacttgaact
     3001 acggattaca tgagtaatta gtttttattt ttgttactgt tttcacaacc agatgatacg
     3061 cctgctagat ttatttgtca accgatttta tatatttttt tatataataa ggtaatttta
     3121 atataaaatt ttatattgta aaaaaacaaa taagggaaac aaacaacaca tttttgtgtg
     3181 ttgcttattt ccctctaact atcgttttct atttgatggt taacctactt aaaataaata
     3241 ctgcaacgac aaacggagat caagcatgct ttgtaacgta ttttacgata attacgactt
     3301 tattttatta ctctatcttg ctgccatacc cgtctatccc taatcatctt tggtgaattt
     3361 tttcaaagtt agcataacaa aaataagtat tttttcctta gttaatcaat agtatcattt
     3421 ttttcatatg atttaattca tcaattaatt ttttgttttt attaatttta gcaaaagcta
     3481 gtgcatcttg aatcaattcg agcacttctg ccttcgactt atttttagcc tgtgcgttct
     3541 tcgctaattg ggcagcagct cgtgctaagt aataagtcaa atggttatcc gaacaaatat
     3601 cgattgcgta atttagtagt gcatcacttg tttccaaatc accgtcttgc gcaaaaaaaa
     3661 caccacaatg aaagacgata tttaacaccc gccagatatc ttctgttgta cgaatcggat
     3721 acgtataaat tttttcaaac actcgattga aataaacttc tgctttttcc aactcttttt
     3781 ctctagcata gaccattcca atacctgtat atgccaataa acgaaacaac gtgttggctg
     3841 tttctgccat caacaactga tcaaaatgga acaacacatc cgtaattggt gcattcgtaa
     3901 aaatcatttc aaatcctttt agatagtagt aacgtgcaat cagctcagac tcgtcaatta
     3961 ccttgggatc aatggctgct aaaatctgtg tcaattgttc atattcactc gtaatcagtg
     4021 aaaactctgc tttttctagc tgttcatcaa gttgtgtatt gatcacgttc atcgaagaaa
     4081 aaagttcaac taacggaaga ttgattcgat tacataattt gattaaaatt tttaacgaag
     4141 ggacttgacc attgttctca aatttactta atgttgcttg tgtacaaatt ccttcagaca
     4201 cttctttttg agataagttg gcagcctttc ttgccgtaat aaatttttca atgttcatta
     4261 ttttctctcc aatcagacct cacacgaaaa tcatttctgt ctcgatttta gcatgttttc
     4321 tttgggaatg ctttaggttc tgttgcaaag ttttaaataa agaataaagt cccttacggt
     4381 atctatgatt taagct
//
LOCUS       DARBEC010000135         4345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.142, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000135 DARBEC010000000
VERSION     DARBEC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.142"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(387..1853)
                     /locus_tag="SCL80_002656"
     CDS             complement(387..1853)
                     /locus_tag="SCL80_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108490.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKKITEPETVVQP"
     gene            complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCL80_002657"
     CDS             complement(2144..4228)
                     /gene="cadA"
                     /locus_tag="SCL80_002657"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEG1108491.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
BASE COUNT         1309 a          927 c          697 g         1412 t
ORIGIN      
        1 aataaatggt gttcaatcag ctgaccttca gcattagctc aaaaagcggc aaaatatgag
       61 gagctatttt tccgtttgtt tctctaatac tcaggtcaaa tcgctgattt tacaacctct
      121 tccttaaacg gtgttccaaa gcttgcatct gcggtaataa gcccagccaa acgaaaaata
      181 tgaggggcta tttttgtttg gctgttctta ttacttgatg caggacagct ttttcacaac
      241 ctctgattca cggtgttcca gaagtagctt cttcgaaaaa caagccgaaa cttcacaaaa
      301 atttgagaag caattttcgt aaattccttc ttatttcttg aagctgacca cttctgtcac
      361 aacctcttgt aacaagtatt tctattttaa ggctgaacca ctgtttctgg ttcagtgatt
      421 tttttgtttt tgtctcgacg agcaattgct ggcaagatca tacctaataa gatcaagacg
      481 attggtgtga tgatattcgt aaataattgg aaccaccatg ctttaggatc agcagtataa
      541 tctacttttg gcaccattcc taggatacat gcaaacgcag tgaaggcaaa gcaccaagca
      601 ccaaagataa aaccgatttt tggattttta acaaatttat attcagaatt gaatttctta
      661 taagctttat tcaacatcat atatgccaag aacacccata ggtaacgcat tggcatgaca
      721 actgagttca agttcgtcaa ccattttact aattcattca tatctttgat accaaaaatc
      781 ggcaacaaaa taatgattcc tactagtata cctgtcagaa gataaccatt caccaatgta
      841 cctttacgat tacgtttacg tagccagttt ggtacaaatt ctggatccgc atctgctaac
      901 aagattttta gtggtgcatc aattgaaaag gctaatgcgg agatttgtcc aatcatgttt
      961 gtcaatgcgt aaatgatcat caatgagtta ccgataccat agtaattacc taaaatttga
     1021 aaagcactgt atgccccatt tgccatcaag tctttaggaa tattatcact agcaaacagc
     1081 atacccattg cgactgagcc taatacagca caaatcccta ccattgctgc taagaagatc
     1141 atccctttag gaaattcctt tgcaggattt cttgtagagt tgacataagg ggatattttt
     1201 tccgcccctc caactgcaaa tactagcatc gaaacggttg tgaaataact gaaatcaaat
     1261 gttggaatat atgttttcac ttgattcata tgtggtgtgg caaattccat cgttggattg
     1321 atgaacggtg ccgttacagc taaaagaata aataagatcg acatgacgaa catcgctgtt
     1381 ccggccagtc cgccaatcac tttcaaagta gaaagtcctt ttgttgataa atataaaaag
     1441 gcaagaaata taataagtga aataactgct accatttgta ctgacatcgt atttacgata
     1501 tttccatttc cttggactgc ccaaccacca gcaatcagaa ttgcttgagg cttttgtgcc
     1561 aaataaggaa tatggacgac ccagtaagtc caagcggcat agtatgccag tcgtttatta
     1621 gatgtcgtat tttcgaccca tgaactgacg cctcctttac tatccttgaa ggctgatcct
     1681 aattgtccaa cgatcaatgc gtatggaata aaataaatgg ccaaaatcaa tagccaagag
     1741 gtgacaactg atactccttg ctgagcatag ttattaacta cattccccat gccccaaacc
     1801 atgttaaatg caattaacgc aacagtaaac cagcgtaatt gtttgttgtt cattcgttct
     1861 ctcctttatg ataaaaattt gttatccaga tgtaatcata ttcaattttt aacaaactta
     1921 caagacagtg ttattttatg tgtgtattct tttatttaat acacatcgtt ttttcatatt
     1981 ttttccatca aaaaaataag ttattttctt ttatttcatt gaccttattg aatttcaagc
     2041 tattttgaat tttctgcaaa attaaaaaca ttagttattt tataatccta tctattttct
     2101 ttacattttt cattaagaaa aggatataaa aaaaagagga cgttcattcg tcctcttttt
     2161 ttacatgcaa gcttcgcatg gcattcaaaa cagctaatac tgtcacccca acatctgcaa
     2221 atactgcatc tcccattgat gcaaaaccaa aagcacctaa ggcaagtacc aaaattttta
     2281 tcccaatagc aaagacaatg ttttgtttga cgattttcaa tgttttcttt gctaaatgga
     2341 ttgcttctgc aatcttcgat ggttgatcat tcatgatgac tacatctgct gcttcaattg
     2401 cagcatcgct tcccaatcca cccattgcga ttcctacatc tgctcttgct agcaccggtg
     2461 catcgttgat tccatctccg acaaatgcgg tctttttatt gtttctttgt aagatttcct
     2521 ctaaccgctg cactttatct tcaggaagca attcgctata tatcttgtcc acgcctactt
     2581 gcttcccgat atgatcagca atttttttcg agtcgcccgt caacataact gtgttcttca
     2641 caccagcctg ttttaattga accaatgcgt ctacggcatc ttctttcaag gtatctgcaa
     2701 taacaagata accactgtaa ctttgatcga tcgctagata aagcaatgta ccgatttctt
     2761 gacttaaagg aaaagagatt tcaaatcttt ccatcaattt gtgattacct gctaatactt
     2821 gtttgccatc aacttcagct aagatccctt cacctgcaaa ttcctggata ttagttgttg
     2881 gtaaaagcgg acctgcgtag ccttctaaaa cagattgagc aatcggatgt gtagaaaact
     2941 gttctgcact cgcaatatat tgcatgaatt tctttggatc gatttttgta tcgatcgtct
     3001 ggatcgaaaa gtcacctttc gttaatgtgc cggttttatc gaataccact gtctcggttt
     3061 gcgcaagcaa ctccaagtaa tttgaacctt ttactaatac accgatttta cttgcgcctc
     3121 cgataccgcc aaagaaacta agtggcacgg aaatcactaa tgcacatgga catgagatca
     3181 ctaaaaatgt caatgctcga tagatccatt cagaaaacgc cgctccagaa acaacaagtg
     3241 gaggtaaaac agctaataaa acggccaatc ctacaacgac tggtgtgtaa tagcgcgcga
     3301 atttagtaat gaaattttca gctggtgctt ttttactgct ggcattttct actaattcta
     3361 ataatttgct gatagtagaa ttttcataag atttttctac ttgaactaaa aatggtttat
     3421 ttttattgat aaatccgctt aatactgttt caccaacata tacagattta gggactgatt
     3481 ctcctgtaag tgctgacgta tcgactaatg attctccttc gatgatcgtt ccatctaacg
     3541 gtacacgttc accaggcttc acaagtacaa actgaccgat ctgcacgtct tcagggttca
     3601 cggtcatcaa tccttccgtc gtctgcacgt gagctgtttc tggacggatc gccatcaatt
     3661 cgcgaatcga ttttcttgaa tgactgactg ccaatccttg gaaatactcg ccgatttgat
     3721 aaaatagcat cactgctacg gcttctggat attcaccgat caacatagcg ccaatcgtag
     3781 caacagacat aagaaaattc tcatcaaaga tttttccttt gacgatattc atcactgcct
     3841 tcttcactac atcatatcct atcagcagat aaatcaataa ataccctatc aagcgaattg
     3901 gttcattaaa atcaaataat agcaataaag cgatacctgc aagggaaaat aaaatccgga
     3961 acagcgtatt tctctctttt tcatttccat gttcagatac tttttcctta tctatttctg
     4021 ttactttgac atctggttct aattccttca ctactttttt tgtttcttgt tcaatcgtag
     4081 ccaaatgatc ttcctctgcg tcaatcgtta gttttccaga agtgaagttt accgttgctt
     4141 ccttgactcc attgatcttc tgaactcctt tttcgatttt cattgcacag ttcgcacaat
     4201 ctaaaccttc taatctgtaa cttttcattt taatgacctc ctcgcctcaa catatgaaca
     4261 tctcttcata actaattata aatgaatggt tattcatatg tcaaggtatc cgaaagaaaa
     4321 aatgaaaaac atctgcatcg gaaaa
//
LOCUS       DARBEC010000136         4330 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.143, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000136 DARBEC010000000
VERSION     DARBEC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4330
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.143"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..283)
                     /locus_tag="SCL80_002658"
     CDS             complement(<1..283)
                     /locus_tag="SCL80_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1108492.1"
                     /translation="MSKKGTIINEWEKLTAPSNRRLKYQSLTLPLKMQWSIDRITIVG
                     KLKENIYYHTPNDVLILNFEQLMRLNEGNGYLRSVSNNGWQLLDQHEENI"
     gene            complement(732..2140)
                     /locus_tag="SCL80_002659"
                     /pseudo
     CDS             complement(732..2140)
                     /locus_tag="SCL80_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
     gene            complement(2361..2723)
                     /locus_tag="SCL80_002660"
     CDS             complement(2361..2723)
                     /locus_tag="SCL80_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108493.1"
                     /translation="MGMTLADIKAQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFETFADFERWLSSISDDQQHEKSVDNEWLADEKSAPSERDYPDESDGLFGVIPNKK
                     SQMSESKFTSIFNDVNLR"
     gene            complement(2727..3065)
                     /locus_tag="SCL80_002661"
     CDS             complement(2727..3065)
                     /locus_tag="SCL80_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108494.1"
                     /translation="MVAILEVRCDMDKIDLREETVDRIELMYERLADYLNLPDEDNYS
                     EMLERIMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDESEKEFWLKLMSHVMQLNQI
                     SVINSKEKEM"
     gene            complement(3044..3463)
                     /locus_tag="SCL80_002662"
     CDS             complement(3044..3463)
                     /locus_tag="SCL80_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108495.1"
                     /translation="MTDLSEKMTIKNLADELGVSKNTVRNQLVNSGFDLAQYKENGII
                     MLDKELIGVVRSIYRSKSDQLTGQLPVNNTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            complement(3456..3689)
                     /locus_tag="SCL80_002663"
     CDS             complement(3456..3689)
                     /locus_tag="SCL80_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108496.1"
                     /translation="MTEKQKYFGKKNWLVNLTGDYVVVVFLDSKKMSGKLLDVTQYEL
                     ILKNNKIDSPIIIPKHAVKYIYAVAESSGEAND"
     gene            complement(3686..>4330)
                     /locus_tag="SCL80_002664"
     CDS             complement(3686..>4330)
                     /locus_tag="SCL80_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1108497.1"
                     /translation="FNPNKISQFLASSMKNFIHDLFIEPHFSRADIACDIIDVPDEFI
                     TQYRVVDPVSFKPIYGRSGKLETAYWGSRASERQIRMYNKKLEQERKRKIVPPEITTW
                     WRLELQLRRGKATDWHEMVHESLDSFASPHYFPPNTKVTERIMVKGLLYDHSEWGNLA
                     KNTKKKYRDLLKQESQNDELTNHLRETFAESADDLKKELDTWLLGLDVTEEEEK"
BASE COUNT         1226 a          962 c          621 g         1521 t
ORIGIN      
        1 caatattctc ttcatgttgg tctaacaact gccaaccatt attactaaca gatcttaaat
       61 aaccattacc ttcatttagg cgcataagct gttcaaaatt taaaatcaac acatcatttg
      121 gagtgtgata ataaatattt tctttcagtt ttccgactat ggtaattcgg tcaattgacc
      181 actgcatttt taagggaagg gtcaaacttt gatactttaa ccgcctgtta gagggggctg
      241 ttaatttttc ccactcattt atgatcgtgc cttttttact caaattttgt tcctcctcaa
      301 agaaaaagag acttctggaa agaattttca aaagttcacg taactcttaa aaattcatta
      361 ccaaaaaatc tctagtcttg aagaaatcta aaaatattcg tataatgatt aagaagttac
      421 ggagctagtg cgattagttt ccgtaaaggt cgagaacgtg tccagcgttg ctcgacttta
      481 tttttttgct cttttttcag tttatttatt tgataactca ataatagcac ttctctttag
      541 ctatgcaaat atccaaaaaa taaaaccgca ccctttgaaa agggcgcccc caaacttcgc
      601 taacgctagg ttggccaaaa aatggacttg ccatttttta gcaaatgtca gcttcaggga
      661 ttagtggttt agccgtcaac tccttatcct cggcacaaac cgcctccggt accgttgact
      721 gcacatcgta actaccactc gatctcatct tcagaacgct ctaatttacg ctgtgagacg
      781 ttttgacgat aaaataaggt tttctcctgc tctaacactt ctaattcctc agaaagcccc
      841 atatcctttt ctgcaagacc aaaacggata gacaaacgag tgatcgattt ttgaaatgcg
      901 ttttttaatc gctctaaacg ctggcgtaaa gagcgatttt cttctttcag ctttttattt
      961 tccacttgta aatctgctaa ttgttttcta ccagacaaat gacgctcttc ttccaatgta
     1021 gctacatact catttaattc tgtattttgc tcaagcatct gtaaatattg ctcactcatg
     1081 ttatcagcat ttctcttggc atttgttgct ctcgtagctc gttgatacaa atcaacaaaa
     1141 tcattcccag ataaatgata cgaaccatca ggaagcctct tgaaactttc taaaggaaca
     1201 atatcttttt ggaaaatgac cgccatctct ttttttacat tcagcatatt tttcttctct
     1261 tcttcaatct tcactctctc agaagccacc agagcctttt ctcgctcatt ttcttctctt
     1321 tgcctagaaa aatatacctc aactcatcag aaacgctctc acgctctttt acggccgttt
     1381 gacgcaattc tgaaatctcg ctcatgattt ctttatattc gtgaacatct ttgattttgt
     1441 tagtaccgac taactcacgc tcccaaccaa aatctgccat caaacgttca attgactcaa
     1501 cttcctgatt cctaaaatta ttccaaatca ctcgaccatt ctctttatct gctcgtaaac
     1561 cctgctgttg caaggctttg ttaaaacttg gttgcttttg aacaccccgt ttataccctt
     1621 cagcaacagg aacaatattc aaatgcaggt gcggacttac ttcgtcatta tgaataacag
     1681 cattgtagac ggataaatta ggattccgtt ctttaaattc tttgaaatat tcaactaaaa
     1741 ttttgttggc aatcactcga ttttgttctg cttgttgttc cgatatgcca gtagaagaac
     1801 catcatcacg agtagtacga aaatcatcaa gagtaccaat ttgcacgata aattctcgct
     1861 gtggttccaa ttttttatcc ttgcgaacct ttgctaaata gtcctcgatt tttctatcgg
     1921 ctcttttctg ctttgcatta tatttttcta cggcttcacc gaataattct tcatattttt
     1981 cacgaatatt tgtttgttct aaatagatat tttcatccga ttttgtgaaa tcaatatgct
     2041 tatgccagtc attgattttt tggctatcag ttaactcacg attattatga gaaatgcttg
     2101 tattctttgt tccctttttg aacgaaatac tgaaccccaa gttaaaaccc tccttcggct
     2161 gtcgctaata aaaagagtgt aaacctttct gttactcttt gtcaaagagt aacattttcc
     2221 taattgtgac atggctgtca caattccaaa tgctctccga gggtattcgc tgtcacaagt
     2281 gacaacgaaa aaaggagccg aaagcgtgtc acgctctcaa ctccttttac gctttatttt
     2341 gcttcacgca aaataatcgc ttatcgtaaa ttcacgtcat taaatattga cgtaaattta
     2401 ctctcactca tctgactttt cttattcggt atcactccga ataagccgtc agattcatcg
     2461 ggataatctc gttcgcttgg cgctgatttt tcatcagcaa gccactcatt atccactgat
     2521 ttttcgtgtt gctgatcgtc agaaatcgac gatagccacc gctcaaaatc agcaaaagtt
     2581 tcaaacccaa aacgcttccg aagagccttg cccattgcag cattttcttt ctcaatcaag
     2641 cgttttttct gttggcgaag tttcgcttgt tcttttttta atttttcttc ttgcgcttta
     2701 atatctgcta aagtcattcc cataaactac atctcctttt ctttggaatt aataacggaa
     2761 atttggttta actgcataac atgactcatt aatttcaacc aaaattcttt ttctgattca
     2821 tcagttgttt gcgctaaatg cttatgaaca aattcattag tcaaaactga agattttgct
     2881 aaatcagcat tgataaccga gttgagaaca tccataattc gttcaagcat ttcactataa
     2941 ttatcttcat ccggcaaatt caaataatca gctaaccgtt catacatcaa ttcaattcta
     3001 tcgactgttt cttctctcaa atcaatttta tccatgtcac acctcacttc cagaattgcc
     3061 accattttcg attttttaat tcttgatttt ctagtaacaa ctgatcgttt ttttgttgct
     3121 gaagcagggt caatttttgt tgatcacgaa tcatttctgt taattcagaa atatgtttgt
     3181 ccttctcagc taattgatca cgcaaaaact cattctcttt ttctaaagga aaatcactgc
     3241 ctgtgttatt gaccggtaat tgaccagtca attgatcact ttttgaccga taaattgacc
     3301 gaacaacacc aatcaactct ttatctagca tgataatgcc attttcttta tactgagcaa
     3361 ggtcaaaccc tgaattgacc agttgattgc gcactgtatt tttcgataca cccaattcgt
     3421 cagcaagatt ttttatggtc atcttttctg acaaatcagt cattagcttc tccactactt
     3481 tcagctaccg cataaatata tttaacggcg tgcttaggga taataatcgg agagtcaatt
     3541 ttattatttt tcaaaatcaa ttcatattgc gttacatcaa gtaacttacc ggacattttt
     3601 ttgctatcca aaaaaacaac cacaacgtaa tcacctgtta aatttaccaa ccaatttttc
     3661 ttaccaaaat acttttgttt ttctgtcatt tttcttcctc ctctgttacg tctagcccta
     3721 gaagccatgt gtctaattct ttttttaaat cgtctgccga ttccgcaaag gtttctcgca
     3781 aatgattcgt caattcatca ttttgacttt cttgcttcaa caaatccctg tattttttct
     3841 ttgtattctt agctagattt ccccactcag aatgatcata aagcaaacct ttcaccataa
     3901 tccgttcagt aactttagta tttggcggaa aataatgtgg actagcaaag ctatccaaac
     3961 tttcatgaac catctcatgc caatcagttg cttttcccct tcgtaactgc aattctagtc
     4021 gccaccatgt cgttatttcc ggcggaacaa ttttacgttt tctttcttgt tccagttttt
     4081 tattatacat ccgaatttgt cgttcactgg cacgactacc ccaatatgcc gtttctaact
     4141 taccagaacg cccataaatt ggtttaaaag acacaggatc aactacccga tattgtgtaa
     4201 taaactcatc aggtacatcg ataatgtcgc aagcaatatc cgcacgtgaa aagtgaggtt
     4261 cgataaacaa atcatgaata aaatttttca tggaactagc taagaattga ctaatcttat
     4321 tcggattgaa
//
LOCUS       DARBEC010000137         4277 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.144, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000137 DARBEC010000000
VERSION     DARBEC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4277
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.144"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            110..491
                     /locus_tag="SCL80_002665"
                     /pseudo
     CDS             110..491
                     /locus_tag="SCL80_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303242.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            622..1572
                     /locus_tag="SCL80_002666"
     CDS             622..1572
                     /locus_tag="SCL80_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002821555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEG1108498.1"
                     /translation="MLKTYSLTKDTLLVSSESNIKNSSVVYVYGDEREYIESSEHKYD
                     FKIGNILTFDDRVAYDTMIDKNDEKSLLVSFLFPEVHEGGHLDTLTTSVVFLVFKNKL
                     IIFTKQKLKFIPELLSNMVLRDVNHFVEEVLLKIMQKDFYMMLNDLRGVKEKIDDLDS
                     EISTSGSLKPTFGDLLTLQKYMIALSTTYKANHKALDFIKKNFTTIDDIDFSTKKIID
                     DLYDTSDTMDRIILSYSQYLDKLENMINNMISYQLNMIMKTLTEISIVLTIPAIIFGF
                     WGINVHVPFEKSSYGVLAVLIISAILSLICWLLMRRKTYL"
     gene            2090..2545
                     /locus_tag="SCL80_002667"
     CDS             2090..2545
                     /locus_tag="SCL80_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1108499.1"
                     /translation="MVQQIVLPIKDSSVLKMVQDTLLDSFRAGRRNYTVFQVGKATLL
                     RVSDVMTLKKSDVYNPDGSVKNATFIHDKKTGKANTLYLKPVQQDLLQYHDWLAQENI
                     NSEWLFPSTAHYDLHITEKQFHKVMAHVGDLLGINLEDKEKKLRVRKPF"
     gene            2886..3488
                     /locus_tag="SCL80_002668"
     CDS             2886..3488
                     /locus_tag="SCL80_002668"
                     /inference="COORDINATES: protein motif:HMM:NF013878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108500.1"
                     /translation="MLDPLFTDETFITFGFGHLVELAEPGHYDEKWQNWKLESLPIFP
                     DRYDFEVAKDKGKQFKIVAELLKKANTIIVATDSDREGENIAWSIIHNANAFSKDKTF
                     KRLWINSLEKDVIRSGFQNLQPGMNYYPFYQEAQTRQIADWLIGMNASPLYTLNLQQK
                     GVQGTFSLGRVQTPTLYLIFQRQEAIENFKKEPFFEVEAK"
     gene            3582..3914
                     /locus_tag="SCL80_002669"
     CDS             3582..3914
                     /locus_tag="SCL80_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase I"
                     /protein_id="HEG1108501.1"
                     /translation="MGNQEGIIADVQTKEKKTNSPSSFSLSSLQSKVNQLYKATASQT
                     LKAMQGLYEAKLLSYPRTDTPFITENEFAYLKANFGKYSGFLGLDLEMLLSLIMIRSN
                     NSTQILTN"
BASE COUNT         1509 a          665 c          757 g         1346 t
ORIGIN      
        1 atgacactat cgttcttcat caacgtaagt tagatttgtt gaaagagcag aaaaaaggat
       61 ttctacaaaa aatgtttgtt tgatatttta aatagaaaag agagataaag tgaagttttt
      121 aaattttttg aactataaat atttaaactg ggtattatat ggtattacat tattttttat
      181 tatttattac ttttttaccg atgatgatat ttttttaatt ttagcaatac tttttcttgt
      241 ttcatctaat tttagtgata acttaattga agttcttaca gaaccagaaa ttcggtctat
      301 gacggatgaa gaacaacaga ttattaaagc tatgatttcg gaaaataaga aagacgtagt
      361 agttatcaaa aagattagag agtatacaaa tttaggtgtt ttacaagcca aaaaagtata
      421 tgaaaaaaaa aaaaagggtc tataattaca aattagataa tacattagaa gctggagaca
      481 agattgtcta gataatgtgt gagataaaat ttttggcagg ttatcataca taagacaaaa
      541 tgtttttaaa actgataacg tatttattcc acaacaatta cgcatttatt tctgagaaaa
      601 atataaagca ggaggattac aatgctgaaa acttattcct taacaaaaga tacattatta
      661 gtttcgtcag aatcaaatat caaaaattct tctgttgttt atgtttatgg tgacgaacga
      721 gaatatatcg aatcttctga acataaatat gactttaaaa taggcaatat actaacgttt
      781 gatgatcgag ttgcatatga cacgatgata gataagaacg atgaaaaaag tttgctcgta
      841 agttttttgt ttcccgaggt acatgaaggt gggcatctag ataccctgac gacatctgtt
      901 gttttcttgg tatttaaaaa taagttgata atttttacaa aacaaaaatt aaaattcatt
      961 cccgaactct tatccaatat ggttttacgt gatgtcaatc atttcgtgga ggaagtactt
     1021 ttaaagatca tgcaaaaaga tttctatatg atgcttaacg accttcgtgg agtcaaggaa
     1081 aaaattgatg atttagactc agaaatatca acgtccggtt cactcaaacc aacgtttggt
     1141 gatttattga ctcttcaaaa atacatgata gctttgtcaa caacttataa agctaaccat
     1201 aaagcgcttg atttcattaa aaaaaatttc acaactattg acgatattga ttttagtact
     1261 aaaaaaataa ttgatgattt atacgataca agtgatacaa tggatagaat cattttaagt
     1321 tacagccaat atttggacaa attggaaaat atgataaaca atatgatttc gtatcagcta
     1381 aacatgatta tgaaaaccct aaccgagata tcaattgttt tgactattcc agcaattatt
     1441 tttggctttt ggggaatcaa tgtccatgtc ccgtttgaaa agtcttctta tggtgtttta
     1501 gccgtattga ttatctctgc tatactaagt ctaatttgct ggcttttgat gcgacgcaaa
     1561 acatatttgt aaagtatttg tgatacccta atatgattta aagcagaatt tctttgttga
     1621 ctttttaaaa ggctgattcc tttcaataat ttaatagctt cagcggctgc tcagaagtca
     1681 ataaattgga ctatttttat tcagttactt aggcaacatt tgagatacga ttgcgattat
     1741 aaaaattgat ataccaacca atggctgctt ggaagactga catattagcc tcgatatcat
     1801 cgaataacta aatacagaaa aatacaataa atattatcaa actagggact atactagaaa
     1861 acatcagtac attccttaac tcagggtcta taattagaaa atcgcccctt tgaaaactga
     1921 ttaaaacagc ccccattatc tagtgactag ataatggggg ctgttttttt gttaagtgat
     1981 ataataaacc ataaaagaag tttagaaata cggtcctggt catcaataaa taaacaattt
     2041 aattaattat ttatttaatt atttaatttc agaaaggtat acatgaatca tggtgcaaca
     2101 aattgtccta cccatcaaag actccagcgt cttaaagatg gtacaagata cacttctcga
     2161 tagtttccgt gctggtcgcc gaaattacac cgtttttcaa gttggtaagg ccacgctact
     2221 acgtgtgagt gatgtcatga cattaaaaaa atcagatgtc tataatccag acggctctgt
     2281 caaaaatgca acctttattc atgataaaaa gaccggtaaa gcaaacacgt tatatttaaa
     2341 gcctgttcag caagacttgt tgcaatatca tgattggctg gcccaagaaa atatcaattc
     2401 tgagtggtta tttccctcga cagcccatta tgatcttcat atcactgaga agcagtttca
     2461 taaggtgatg gcgcatgttg gtgatctctt aggaatcaac ttggaagaca aagagaaaaa
     2521 attgcgagtc agaaaaccgt tttgagatat gaacagcatt tgccttcacg ccttagcgat
     2581 tttaaataat agccttgaaa gtgattatgg aaaagccctc tacgctcaat gcaaaattga
     2641 cgtagaaact atttttgatc gattgaaagg tgtatttggt atgtgaagaa cccatgtcag
     2701 aggcaagaac gttgcaccta ctgacattgg aatgatgctt atgagcatga atctaaccaa
     2761 attatccctt gaggctagaa gaaaagcgga agcttttcaa cacaaagcag ccaaaaataa
     2821 aaaacgcgac gaagcaatca catttatgat tattcatcgc gttttttata tttagagcta
     2881 gttatttgct agacccacta tttacagatg aaacgtttat cacctttggt tttgggcatt
     2941 tagtggaatt agcagaacca ggtcattatg acgaaaagtg gcaaaattgg aaacttgaat
     3001 ctttgccgat ttttcctgat cgatacgatt ttgaagttgc aaaagataag ggaaagcagt
     3061 ttaaaattgt tgcagaactt ctcaaaaagg caaatacaat tattgttgca acagatagcg
     3121 acagagaagg tgaaaatatc gcctggtcga ttatccataa tgcaaatgcc ttttcaaaag
     3181 ataaaacatt taaaagacta tggatcaata gcttagaaaa agatgtgatc cgaagcggtt
     3241 ttcaaaattt gcaaccagga atgaattact atccctttta tcaagaagcg caaacacgcc
     3301 aaattgccga ttggttgatc ggcatgaacg caagcccttt gtatacgtta aatttacaac
     3361 agaagggcgt acaaggtaca ttttcactag gacgtgttca aacgcccacc ttatacctta
     3421 tttttcagcg ccaggaagcc atagagaatt ttaaaaaaga accttttttc gaggtggaag
     3481 ctaagtaaaa caagggtcat ttaagggcgt tctaagcccc acacagcgtt ttaaaaccca
     3541 agaggagctt tttagctttt gtttcttcta aacaagctaa aataggcaat caagagggga
     3601 taattgctga tgttcaaacc aaagagaaga aaacgaatag tccgagttcg ttttctttaa
     3661 gtagtttgca atcaaaagtc aatcagcttt ataaagcgac agcgagccaa actttaaaag
     3721 ctatgcaagg actgtatgaa gcaaaattat tgagttatcc aagaacagat acaccattta
     3781 ttacagagaa cgaatttgct tatttaaaag cgaattttgg caaatatagc ggttttttag
     3841 gacttgatct tgaaatgttg ttatcattga taatgatacg ttcaaacaac agcacccaaa
     3901 ttttgacgaa ttagtgaaac tctatgaaaa agacgtagta aaacatgcaa catcctattc
     3961 taatcagctg gtaaagctta attaggttat ataagcttga aaataaaggg tttgtccaat
     4021 atggacaagc ctttttctta tgttttgcca cagatttgcc acacttattg tgtaagagta
     4081 tcaaggatag gtataatttg atactttgtt tttttagtga tgtgcaaata aatttctgtt
     4141 acacgactac ctctagaatg ccctacacgg tcttgaattg cttctagggg tattccttac
     4201 tctgctaaga aactaatatg cgtatgacgg aatatatggg tcgtaaggtt ctgttgcgaa
     4261 gtttgaaaat caaacgc
//
LOCUS       DARBEC010000138         4203 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.145, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000138 DARBEC010000000
VERSION     DARBEC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.145"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(58..1824)
                     /locus_tag="SCL80_002670"
     CDS             complement(58..1824)
                     /locus_tag="SCL80_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108502.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(1784..3537)
                     /locus_tag="SCL80_002671"
                     /pseudo
     CDS             complement(1784..3537)
                     /locus_tag="SCL80_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(3647..>4203)
                     /locus_tag="SCL80_002672"
     CDS             complement(3647..>4203)
                     /locus_tag="SCL80_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEG1108503.1"
                     /translation="YIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGLLSGII
                     FVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVFGYDRA
                     IPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGIWGIIS
                     VLVVASLSMIGVLIGRKVLKKYKK"
BASE COUNT         1427 a          797 c          592 g         1387 t
ORIGIN      
        1 tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc tatttcatca
       61 tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt tctttatgag
      121 atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat caactagttt
      181 tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc gatgcgatat
      241 aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac gtttttcatt
      301 ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat ttttcaaaaa
      361 agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc cgttttctcc
      421 gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt tagctaaacg
      481 agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta tattttccaa
      541 aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc taaacaaaga
      601 agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt catcataatc
      661 atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag cacctactaa
      721 cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg gggtattgtt
      781 atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag gactatctga
      841 tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag gagctaaata
      901 aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt ttattgcggc
      961 tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta agtgaacacc
     1021 tactagaatg actactgcta aactaacata tgagaaaata gaggataagg ccatgactga
     1081 aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt ttttgaaaag
     1141 tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca attctatagt
     1201 ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat tggctttcgt
     1261 ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca aaataacagc
     1321 taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga aaaacagagc
     1381 cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt caacgtcaga
     1441 catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata atggtaaatt
     1501 tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag tactattata
     1561 taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa caaacgatac
     1621 ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta tcagcatcat
     1681 tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa tagaagcttt
     1741 agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa aacttttcat
     1801 tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta tacacttgat
     1861 gtttagccat taattcatta tgatttccac gttctactat tttcccttct gacaagacaa
     1921 gaatttcatc tacagattta atgctggata atcgatgagc tatcataata actgtttttt
     1981 ctttcatcag atgttgaaaa gccttttgta gttcatactc attatctgga tcaatagatg
     2041 cactggcttc atccataata acaatctttg catcttttaa aattgctctg gcaatagcta
     2101 tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag gtatgctctc
     2161 tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg tcaaatactt
     2221 tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc tgattgaata
     2281 gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt gaatagtgat
     2341 ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa cctgaaatta
     2401 gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa attctttttt
     2461 gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa aagctgacat
     2521 tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt agttggtctt
     2581 gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct tcaaacaagt
     2641 gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt aaaaaaaaca
     2701 acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata aaaaaggtta
     2761 taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga acaaaaggtg
     2821 ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt gctttattca
     2881 aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt ccgcggatat
     2941 attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga ataaaatttg
     3001 tattctctgt cattttcttt aacaagaata caccaattac taacatggcg attagaacca
     3061 atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt gttactatgg
     3121 cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc gcgttgtcat
     3181 caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa cttgcttctg
     3241 ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca aggatatgag
     3301 aaaataagcc agaaaaaata taaaataaag ctctaaagtt aaccaagcaa cagtttgaac
     3361 cgcataactc ttagctaaat attcatctcc tacaataatt agagactgaa gaaatttgta
     3421 aatactgtaa tatccataga ctgttaatac gacagaaaaa tcagaaaata aagtggctaa
     3481 ccatccgtag tatttcattt tagggacata actaaataat tttttgtata ctttcatttt
     3541 ttcctccccc gcattcaatt tcttctctta ttcgtttaca actgttaatg ctttccgtaa
     3601 tagcgtaaat tagagatgta caaacagata aaaggtaaac tacgttctat tttttgtatt
     3661 tttttaagac ttttcttcca atcaatacac caatcataga taaacttgct acaactaaca
     3721 ctgaaatgat tccccatata cctaccgcat agtggatcat tgcatccatt gttgcagcag
     3781 attgtccttt atctatccaa tcttgacgaa aacgatctgt aaagaaccat ataggaatcc
     3841 actgtccggc agcactgcct aattgcatta gagcccaaga cgcaacttga ggaattgctc
     3901 gatcatatcc aaatacagat agaataatat cagctaagat cgccatacta atcgaactga
     3961 taaaccaagg taagtacgcc ccgcccatta acgtaaaaaa taacaataaa acaattgtgt
     4021 aaatagtaaa aattcctttt ctagggtagt tatagctaat ataaacaaat atgattccag
     4081 ataataatcc aaaaatcccg ggggatatgc tgtgaccaaa cgccccacca atactactaa
     4141 ccattattga aaaaaagtaa attactaggg aaataactgt agctaaacca ataaaaatat
     4201 aat
//
LOCUS       DARBEC010000139         4101 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.146, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000139 DARBEC010000000
VERSION     DARBEC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4101)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4101)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4101
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.146"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(210..1715)
                     /locus_tag="SCL80_002673"
     CDS             complement(210..1715)
                     /locus_tag="SCL80_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEG1108504.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(1712..2398)
                     /locus_tag="SCL80_002674"
     CDS             complement(1712..2398)
                     /locus_tag="SCL80_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108505.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2591..4012)
                     /gene="gndA"
                     /locus_tag="SCL80_002675"
     CDS             complement(2591..4012)
                     /gene="gndA"
                     /locus_tag="SCL80_002675"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEG1108506.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1130 a          861 c          695 g         1415 t
ORIGIN      
        1 tatttttatt tattattttt aaacacgaat attacagcat tttattgttt tttcgttcat
       61 ctaaaagata cttacattta attaacggat gttattctat ttaattgttc gctagttttt
      121 ttataatgac atccctgaat ttctcacttg cttgtatttt gtaccttatt ctttgttgga
      181 tatataaaaa aacaagtaaa gcacatgctt tacttgtctt ctgcctttgc gataggaata
      241 aagattctaa aaattgtccc ttgccctact acactttctg ctaagatacg tcctttatag
      301 ttttcaacca gttgtttagc gatagacaac cccagaccat ttccaccctt cgttctagct
      361 cttgctttat ccactcgata aaaacgattg aagatcttat taaggtcttc tttgggtatt
      421 ccttcgccaa agtcctgaat cgcgatctca aattcattca gagtggaaga aatagagata
      481 tggacttctt ttcgtgtagt tgaatacttt actgcgttat ccaatatgat aatgattaat
      541 tgttcaaagt gattacgata aatttgcagg gtcacttctt ttgtcaaatc atcatctaaa
      601 gtgatcacat agtctggata taatatcttg aaattattat aaacttgata ggtgacttct
      661 tttcctttag ttgtttcatt cgcataatga acatctactt gttctgctct tgataagtct
      721 agcatctctt gaactaagct tttcatacgg ctgatttctt gcatgctggc gcttaatgat
      781 tcttctaata tctcaggatc atctttcccc caacgattta gcagactaag atgcccttca
      841 ataattgcaa caggtgtcct taactcatga gatacgtctt ccacgaattg ctcttgttgt
      901 tcaatataca aacgcatacg gtcaagcatc tcattgaata tttcagctaa atctgccaat
      961 tcatcatttg tgtcgatttc aggcatgtgg atatctgatt gcggatcttt acgtatagta
     1021 tccattgtat ctctgagtac cttgagtggt tttaagaaat aagaagataa aaagaaacct
     1081 aaaatactac ttaagatcaa agaaacaact tctaaaacaa taagagtcaa taatagctta
     1141 tttcgaatat catagaaaga agataattca tagaagcttt gcgcatagcc gatcttttct
     1201 cttgtacctt tagaatatat aggctggata gataaaaagc cagtttttcc ttctagtgta
     1261 acgatcgttg gaactttacg tgtagtctga atcaggtttc gactgttttc atgggtttta
     1321 aaaatcaagt tctcgtccaa atcataaaca taaagatcca ttgatggctg ccctagctca
     1381 gctataaagg aatcgatctt cattaagttt ccttcgacca gtgaagtatg atctacgacc
     1441 tcatctccgt catttgtcgc tttcgtcaga ttacgatacg tatttactaa tgtcagttct
     1501 tcatccgagt tagccaaacg agaagctact tctgaaatcg tacgctctac attgtttctt
     1561 tcttttgcca caatcagatt gatagatgat ttataggtaa tgacagcgaa aattgtaaac
     1621 acaacgaata tgaagaaaga actcgcaaag gcccacttga ttgtcaagga gggccctttc
     1681 agttctcttt tttccgattt tttcggcttt atcacgagcg catcacataa cctgttccgc
     1741 gtacagtttg gatatagctt tcctccccgg gaacgtcaat tttgttgcga agataacgaa
     1801 tatatacgtc cactacgttt gtttcaactt ctgtttcgta tccccacact ttatttagta
     1861 aaacgtcacg tgctaatacc acgttcacgt tttccattaa agtaagcagc aactcgtatt
     1921 ctctttttgt tagttcaatt acttcatttc cgcgacgtac gacacggttt tctttttcga
     1981 tagtcaaatc gcgataagta atagtagttt gtttagccac atttttatcg ccttcaatat
     2041 cgattcgacg caacaatgca cgaagacgtg ctaataattc ttcgatagca aatggtttga
     2101 cgatgtagtc atctgctccg tggtcaagtc cagatacacg atcgatcaca gaatctcttg
     2161 cagtcatcat aatgattggt gtatttttga cttggcgtac acgtctgcag acttctaaac
     2221 cgttcaattc tgggagcatc agatccaaca atatggcatc ccactcatta tttaatgccg
     2281 catctaaacc agttcggccg ttgtagtgga cttctgcgtt atacccttca tgcttcaact
     2341 ctagctcgac aaatcgtgcc aagttctttt catcttcaat gattagaata ttgctcattt
     2401 attattttat cctttctatt ctactggctt tttaaaaact ctatcattat accgataata
     2461 tgcttaaaaa tctatagtca ttttatcatg gatagcaatt ttaaaaaaga gctgaagtct
     2521 actcataaca atcgacttca gctctttcgt cctcttttta ttttctcatt tcctaacagc
     2581 cgaaagacta ttattcttca tggtaccatg agtaatggaa gattccttct ttgtcgacac
     2641 gctcataggt atgtgcgcca aagtaatcgc gttgggcttg gattatattt gcaggcagac
     2701 gttctgctct gtatgaatcg aagtaagcga tggctgatga gaaagtcggt acaggtacgc
     2761 cagcttggac agccaaggca acgacgtctc tgactgattg ctgatatttt ttcgtgattt
     2821 caacgaagta atcatctagc aataaatttt cgatctcagg atttttgtca taggcatccg
     2881 taattttttg caagaatcga gctcggatga tacatccagc gcgccagatt tttgcgattt
     2941 caccaaatgg caagtcccaa tcaaattctt ttgaagcgac acgtaattga gcaaatcctt
     3001 gggcataact catgatttta ctgaaataca acgcttctcg gattttttcg accaattctt
     3061 ttttgtctcc cgtgaactca aaatcgttcg tacgagacaa aatcttgctt gcttgtacgc
     3121 gttcttcttt ataagcagaa atatagcggg caaatacaga ttcagtgatc aatggaagtg
     3181 gcacacctaa gtcaagcgcg ctttggcttg tccatttacc tgttccttta ttcccagctg
     3241 catcaaggat cacatctact actggtttac ctgttccttc atcgtcttta cgtgtcagaa
     3301 tgtcagcagt gatttctatc aaatagctgt ctaattcgcc ttgattccat tctttaaaga
     3361 tatcagccat ttcttctact gaaaggttta aaatattctt cattaagtca taagattcag
     3421 caatcagttg catatctcca tactcaatac cgttgtgtac cattttcaca taatgtccgg
     3481 ctccatttgg gccaatatat gtgacacatg gtgctccgtc ttctgctttt gcagaaattt
     3541 gctctaagat tggtgcaact agcgcatagg cttctttttg tccaccaggc atgattgaag
     3601 gtccttttaa agctccttct tcaccaccgg aaacgcctgt accgataaag ttgatgccag
     3661 agttggctaa ctcttcatta cgacgcatcg tatctttaaa gaatgtattt cctccgtcaa
     3721 tcaaaatatc gcctttatca aggtgaggaa gtaactcttg gattgttgca tctgttgccg
     3781 gtcctgcttt gaccattagc aagatccgac gtggtttctc gatagattct acaaattctt
     3841 cgattgtata tgttgctttg aaatttttat ctgggtgttc ttctactaca gcggttgttt
     3901 tagcccctgt acgattgaat aaagccactg aatagccgcg gctttctatg ttcaaggcta
     3961 gatttttacc cataacggcc ataccgacaa cgccgatttg ttgttttgac atatgaactc
     4021 ctcctacata cgggatttct ccctaatctc ttacattata gcttaacacg gctacattgc
     4081 aaactaaaga acaacatatt t
//
LOCUS       DARBEC010000140         4036 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.147, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000140 DARBEC010000000
VERSION     DARBEC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4036)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4036)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4036
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.147"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(242..391)
                     /locus_tag="SCL80_002676"
     CDS             complement(242..391)
                     /locus_tag="SCL80_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108507.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            853..1065
                     /locus_tag="SCL80_002677"
     CDS             853..1065
                     /locus_tag="SCL80_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108508.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1343..3142)
                     /locus_tag="SCL80_002678"
     CDS             complement(1343..3142)
                     /locus_tag="SCL80_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEG1108509.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3266..3862)
                     /locus_tag="SCL80_002679"
     CDS             complement(3266..3862)
                     /locus_tag="SCL80_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEG1108510.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1449 a          711 c          689 g         1187 t
ORIGIN      
        1 atattaaata ttatacagat aataacggaa tattatcatc gaaacagcta acaaaattgt
       61 cataaaaaac gagatggttg atattagaac ttgtgttttt gtttaggtct agagataaag
      121 agagacaatt tccaaatata tgtaatgaat acaaaaagac gtttgtcgcg gattaccgtt
      181 acaaacgtct tttattgatt ttccgagatc tcatcagtcg ccatttggaa tggttcactc
      241 cttatctaga ttcgtttaat gctcggactg cttgttcgat tgtaatacca aacgcaacgt
      301 tattttcgtt ttgtgcatca atcgccataa aacggttaga agttgtatca aaagctacgc
      361 ggtattgcat atttgttttt gtaaacgtca taaaattcat ctcctttgta atgtaattta
      421 gttcttgaaa gtttccttta aactttcgaa cttataccaa gtttagcatc gtacgttttt
      481 tgatgcaatt aaaaagcatt tatcattttc cttatttatt ttttcttaaa tggtttagct
      541 attttctttt aatccgataa gtagcccaat catcaaggtt acaaatacta ttgcttgaat
      601 ataaatcata tgctcacctc tttataaata acttagcaat ataaagaaaa aaacgcacga
      661 acaacatagg aatataaaca aacgtttttg tcacaacgtc taagatcgtg ttaaaaaata
      721 gttatctaaa aagcagaaag atactcaaaa taaaaaataa aaaaatggat aaaaacgttt
      781 tcaaatacaa ggtaatttgt tattataaaa taaagaaaaa acaaaataaa aataaagaaa
      841 agagggaatg aaatggtaaa agcagaagta ggaagatctt atatatgcaa accgatcgga
      901 gtatctcatg aactaatcgg ctgtattgaa cgtacatata tcaacacggc tgtcgtgtat
      961 gttgaaagtt atgaccaacg agactatcaa cttatcaatg aaagcaagta tcgcatgctc
     1021 gtaaaattct gtgatattag cgcacctgct gaattggttt cttaaacagt atttttgaga
     1081 attcgcaaga actcccttat aatataaata aagacagaag ggagcgagaa gatatgccaa
     1141 aaagaaaaag ttcagggaaa atgggaatta ttcttgcagt gatctctgca attcctgcta
     1201 ttttagctat tgttcgtgcg atgaaggaat caagagaaag ttaagcacac attatgagcg
     1261 tcaaacaaaa aacgcatata cctgcggtgg caggttatgc gttttttgtt gtataaagac
     1321 ttttttcatt aacgattttt gattagccga ctcccaaaac aaaatttagc agtttatctg
     1381 aataagtgat ttcttcatct gtttgtttca cactttcatt tagtagtccc atctcatcgg
     1441 ttatgatacc atccacacca taaaacatca tgcgactcat agcatctgaa tcgttgattg
     1501 tccatacata tacttctttt ccgtcttggt gggcagaatt aatgaagtta cggttgatcg
     1561 tactatattc cattgtcaga aaatcagcag gcgtcacagg gggcccaaca atattgaaag
     1621 gtaagatgta gcccacataa aagtttggtt ctgcttcttt taaatctgaa acggtttggt
     1681 acgtcaaact ttgcaaaata tgatggttct ctaagataac tttgcggtat ttatggacaa
     1741 atcgttcgac aaggtcatcc gaatcttttt tagaggtctt gatctcaata agtaattttt
     1801 ggtttagctt atgtgctttt tccaaataag aatcaaaaga acaaacaggg gcttgcactc
     1861 cgttttcagt aactgtaaga tttgtcaatt ctttcaatgt aagatcctgt ggtgttccat
     1921 ttacccctgt taagtgacga agatttgcat catgcatcac tacgaaatga ccatcttttg
     1981 tttcctgaac atccatctct acataatcag gatgatagtc tctgcttgtt ttttcaagtg
     2041 cttctaaagt attttgcgcc ccattttggg tactcacacc tctgtgagag acagtcactg
     2101 gttttgtctg aacagctgaa gtcagataat ccatattgta aagacagacg ccaataccga
     2161 aaaaaactgc aaaaagagtc aaaccagtgt ttttcagcgc agagaaacgc aagttggggg
     2221 cttgtgggat aaaccactta gggatctcag gcaagaagcc ttcgtcatcc atgaaatcaa
     2281 caatgatata aaaaatccct acggtagaca tcacgatatt gaataataaa atcccttgta
     2341 aaagcgtcat agcaaacaca gcgctgatca atgcatagtc tgggaaaaat tgttcaacca
     2401 tactttgtgc caaaataaca acgatatatc ctaaagaaga aaggagtaaa atcgtaccgc
     2461 caatcacaat aaattggccc gtgatggcca aaagacgcga tttggtcaat gaccagcttt
     2521 cccgaatggc tgctcgaaaa ggacgatctc gtagaatcat ttccggtaaa gcaaaaatca
     2581 gtcgaatccc aatatagcca aggaaaatat aaacgagtaa aaaggaactg acgatgatcc
     2641 aacgattagc aaaaataaaa tccataatga aagcggggat ttttattttt gacagtagat
     2701 cagaattaaa acttaatccg ctgatcggta gaatcaataa aaaataagca aggaaaaaca
     2761 aaaaagtaat gggtctcact ttcttcagct gaagtatcgt caagtgtaag agttgcttca
     2821 aagagattgg ttctttcttt ttaataaaaa aaacactcat caaaagaaaa gtaaattcga
     2881 aataaactaa cagtaaaatc gtcatcaaaa ctacgattaa cgagagtaga accccaggat
     2941 gttgagaaaa aattgaagga atattgtcat aagacaaata gtcaattgcc cctcggtgta
     3001 aaataaaacg ggtcgtactg ctcaataagg gtaaacaaat aaataaaata aagccatgca
     3061 tcagcaagac atcacggaaa taagcttgtg ttccttttaa aaagtcccaa gcattaccta
     3121 agctacgttt caaataattc atcctatcac ctgctcttca cattgtagcg caaatctaat
     3181 gaaaaaggaa agggtgcagt gtgttttgca gaccctttcc cagatttaac attgtcaatc
     3241 aatgactaat tttacaaaat aaatattatc caagaagacc acttagatat tgacctccat
     3301 aaatcaaggc catactatat aagaaaatcg acagtggttt agcaatcaaa atgattagtg
     3361 tatatcgttt cactgacatg tttgtcaatc cagccatcaa acataacgca tcgtcaggag
     3421 caactggaag aaaaatagct agagcaaaca gcttctcaaa acggttttga ttatccagcc
     3481 agccaatata tttgttatat gtcttatcgc taatcatgga aagtatgaat ggctttccat
     3541 aacgacgccc tagcagaaaa atgataatcg aaccaatgca gattccaata tagttataga
     3601 taaatccagc ataagggcca aatatcagga caccagctgc agtactgatc cctccaggaa
     3661 tgattggaat aactacttgc aggatttgga tgaagacaaa aataatcggt cctaaaatga
     3721 tggaatcacc tactaatcct cttaacgcat tcaaatcttt gaaaacacct aaattaataa
     3781 aataaatcgt cagcgcgatt gataggccta atccgataat ggatatgaaa tttatcagtt
     3841 ttcgagaggc agcaatattc attattcatc aactccttag aggttctttg ttaactatac
     3901 tttactagaa aagcaactaa ctgcgccatt ttttgatatg acagaaaaca aacaattttg
     3961 taagttcatc ataagtataa aagattgatc tcaaaaaaac atccaactaa agacttattt
     4021 aataacttgt tttttt
//
LOCUS       DARBEC010000141         3950 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.148, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000141 DARBEC010000000
VERSION     DARBEC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3950)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3950)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3950
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.148"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..727
                     /locus_tag="SCL80_002680"
     CDS             <1..727
                     /locus_tag="SCL80_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1108511.1"
                     /translation="IDFLSTPFDEESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYG
                     LPVLLSTGMADIHEVSEAVDCFIDSPVTILHCTSNYPASSEDINLLAINTLKEMFGIP
                     VGYSDHSLGYDVGICAVAMGAKVIEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNT
                     EIFLGDGVKRAMPSELSTKEVSRKSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLY
                     QYIGKKVKRPISADSVLLEEDVE"
     gene            724..1872
                     /gene="neuC"
                     /locus_tag="SCL80_002681"
     CDS             724..1872
                     /gene="neuC"
                     /locus_tag="SCL80_002681"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEG1108512.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            1845..2522
                     /locus_tag="SCL80_002682"
     CDS             1845..2522
                     /locus_tag="SCL80_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEG1108513.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            2512..3654
                     /locus_tag="SCL80_002683"
     CDS             2512..3654
                     /locus_tag="SCL80_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEG1108514.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            3667..>3950
                     /locus_tag="SCL80_002684"
     CDS             3667..>3950
                     /locus_tag="SCL80_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1108515.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINL"
BASE COUNT         1427 a          515 c          789 g         1219 t
ORIGIN      
        1 aattgatttt ttgtctactc cttttgatga agaaagtgca gactttctaa aacaaattgg
       61 tgtacaagga tttaaaattg gctcaggaga tttaacaaat ttaccttttc ttagaaaaat
      121 agatatgtat gggttgccag ttttattatc tacaggtatg gctgatattc atgaggtcag
      181 tgaggctgta gattgtttca tagatagtcc agttactatt ttacattgta cttcaaatta
      241 tccggctagt tctgaagata ttaatttatt ggcgattaac acattaaaag aaatgttcgg
      301 gatacccgtt gggtactccg atcattcatt gggatatgat gttggtattt gtgctgttgc
      361 gatgggtgct aaggtaattg agaaacattt taccttggat aaggaactac ctggtccaga
      421 tcataaagct tctttagatc ctcaggaatt tgcagatttt gtaaagcata ttagaaacac
      481 tgaaatattt ttaggtgatg gtgttaagcg agcaatgcct tctgaactta gtacaaaaga
      541 agtatcaaga aaaagcctag tggtcactga gaatttaaat gtgggagata ttcttacaga
      601 gaaatctctt acaataaaac gtcctggaaa tggaatagct cctaagtttt tatatcaata
      661 tattggcaaa aaagttaaaa gaccaataag tgcagatagt gttctgcttg aggaagatgt
      721 tgaatgaaaa aaatacttat agtaagtgga acacgagctg attatggaat atataaacct
      781 gttgctgaga atttactaca agataattgg gatgtatcat ttgttgtttc tggaatgcat
      841 ttatcccata aatatggatt tactaaagaa ttgatcttag ctgacggatt tagaattagt
      901 ggagaagttc aaagtttgtt tttagagaac aataagggaa acatggctcg gtctgtggca
      961 ttagagataa atggtttcac aaatatattt gaacaaacta gccctgattt tgtattatta
     1021 ctaggggata gaggagaaat gttggcagct gcgatagctt gtttgtatct tggaatcaaa
     1081 acagcacata ttcacggagg cgaagtgtct ggtactgtag atgaatcaat aagacatgcc
     1141 ataacgaaaa tagcatctgt tcattttgct gctacggaaa aaagcaaaga gcgtttaata
     1201 aaaatggggg aagattcttg gagagttttt cccgtcggag ctcctagaat agatactatt
     1261 ttaaataata gattacctag ttttagagat ataaaagaaa aatatcatct aaaatttgaa
     1321 aagaaaaaat acgttttgca agtatttcat ccagttgtaa ccgaactaga aaatatagaa
     1381 aaacaaattt tagctttgat caattcaatg gaacaacaat ctcaaccaat tatttgcatt
     1441 ttacctaata gtgatgctgg tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa
     1501 acggatatta tttatattac gaatttagag ccggaggaat acctgactat attaaaagag
     1561 tgcaaattta tgatcggaaa ttcttcttct gggataattg aagctgcatc attcaaaata
     1621 ccagtaataa atttaggaac cagacaaaat ggacgagaac aatctaaaaa tattataaat
     1681 gcaaatctaa atgaaaatga aattgaaact gctttgaata aaatacaaga tcctcagttt
     1741 ataaaaagct tgaaaaatgt taagaacatc tacggtgacg gcaacagttc tagaagaata
     1801 acaaaaacat tgaatcaaat attgaatgat ggggaagata ttaagtggat tcaaaaacaa
     1861 atagcttatt aaaatatact agtgatccat acattattat tggggctggc ggacacgcaa
     1921 aagtcattat tgaaacctta agattagaaa ataagttaat atatggattt tttgatgata
     1981 agcaaaataa aatgcaagat ctgaaatatc ttgggaaaat aaacgaattg aatagctggg
     2041 cggatagcaa aaaaaaaatc tataaattta ttgtagctat tggtgactgt gtagtaagaa
     2101 aaaagattat aaaaaaattg aatatgccaa gtgagcaata tggaatagct gttcatccaa
     2161 gtgctatagt ggcaaaagaa atttcaattg gttatggaac tgttattttt gcgaatgctg
     2221 taattaaccc agatgctgta attggagagc atgctataat aaacacaggt agcattgttg
     2281 agcatgattg tagaattaat aactatgttc atttatctcc aggtgtatgc ttagcgggtg
     2341 gagtacatgt aggtgtagga actcaaatag gaattggttc tcaatgtatc cagatgaaga
     2401 aaattggatc ttggtgtatg ataggcgcag ggagcactat tgttaaggat attccctcac
     2461 attccttagc ttatggcaat ccagctaaaa ttaagaaaga aggaattaat tttgaattct
     2521 agaattttat tatcaagtcc tcatatggga ggaacagaag aaaaatatgt tgctaaggct
     2581 tttaaagata attggatagc gccattaggt gaaaatgtgg ataaatttga agaaagtatt
     2641 acaaaatata ctggttctaa gggcggagct tctgcgttaa gttcaggtac agctgccata
     2701 catcttgcct taagactgct taatgttgga aaaaacgata ttgtctttgc tccttcattt
     2761 acttttatag ctacagtaaa tcctatttta tatcaagggg ctgtaccagt gttcattgac
     2821 tcagaaccta atacatggaa tatgagccct gttgcactag agagcgcatt agaagaagct
     2881 gaaagaaaca accaattacc taaggcaatc atcgtagtga atttatatgg tcaaatggct
     2941 gattatgaac gaattttagc tattgcaaac caatttaatg ttccagtgat agaagatgca
     3001 gcggaatcgt taggttctac ttataaggga atttcgtcag gcttatttgg gacaattggg
     3061 atctattcat ttaacggtaa taagatcatc acaacctcag gtggaggagc tctagtgtct
     3121 agtgataaaa aatatgttga gcattcaaga tttttagcaa cacaagcaag agatcaagca
     3181 ccgtattatc aacatagcga aataggttat aattaccgtc taagcaatat ttctgccggt
     3241 atcggacgtg gacaaatgga agtattgaat gatagagtag ctaaacgaag agaaattttc
     3301 aatcgatata aggaagcttt ttctggaatt gatagaattg aaatgatgcc agaagtggat
     3361 ggcatggtgc caaatcgttg gttgtctact ttaacgatca atgattataa ttctattgat
     3421 caagtagtta acgtaataaa taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg
     3481 aaaccactac atttgcaacc agtatttcaa ggggtaaaaa tgtattacca tgataataat
     3541 cttagtgtgt cagaaaagtt atttaatagc ggcttatgct taccttctgg aagtaatatg
     3601 acagatagtg aacaagaatt tgttattgat gagtttaaaa aaatattcaa gtaggtgtga
     3661 tattttatga acataggaat tattggttta ggttccatgg ggaaaagaag acttagactc
     3721 attcaagaac actttaatga tattttacta tgtggtgttg atactaatga ggcgagacga
     3781 aaggaagttg aaaaggaatt tggaatacca acttataata gtctaacaga ggcaaaaaat
     3841 ttgtttgaaa taaattcagt ttttgtttgt acatccccta taacccatga atttattata
     3901 aaagaggctt taaatttaga ttgtaatatc tttacggaaa tcaatttatt
//
LOCUS       DARBEC010000142         3883 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.149, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000142 DARBEC010000000
VERSION     DARBEC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.149"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            218..673
                     /locus_tag="SCL80_002685"
     CDS             218..673
                     /locus_tag="SCL80_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108516.1"
                     /translation="MNAINWHEFVNYMESLLDDLKSFSIADAVKHGLPVSTTMKLRGL
                     KIPDEIIKLRTKTIIETAKVVDNMNHELKGKKTTYEKDFERFLKSVIGKYLGKKSFSE
                     LRDEEIVPTPMVSKIRKNKAEGNYYLGTIAKIIDYLENKEQKKGTKKGR"
     gene            792..1850
                     /locus_tag="SCL80_002686"
     CDS             792..1850
                     /locus_tag="SCL80_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliS"
                     /protein_id="HEG1108517.1"
                     /translation="MEKNVNSTSTKDQYSIIGGLLGDKDKRTRHGLRIMLATFLGVLF
                     LLTGLGSYIYQLGNYNYYLVNNTTPIGSSLEFARSGAEITISDVWTDQNRDVTVVKLG
                     YSQNARKLLSTQGKNYNLYMATKEKDRPKKLEMSYGMLSTDGDAFLFLKGKLEERAYQ
                     VFIANQVSLISGDDTADDDTKVNVNEADSVTKALSQYSMNDVNNEGVISFGKKAKPVN
                     TPDNINFRINPYSDSTNVYQGSFLTKDGDIDYSKVVAVTSKNNVVNKLEDQLNASNTN
                     IKKIEATKAEYLERLQQNPDDKDSQESIRQLDSRMETEIKAINDTQKLIDQYKNADFN
                     KDSFGDMKTTFKYLTLTD"
     gene            1892..3841
                     /locus_tag="SCL80_002687"
     CDS             1892..3841
                     /locus_tag="SCL80_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEG1108518.1"
                     /translation="MENSTEFFKNIQTEFNHALTPMKPPKHKLVGRDDELHELSLILN
                     RPETPQAILLGEAGTGKTALASEWAERQRQDGKKVQVYKLSIGQLRVGGDDVLQTRLE
                     TLIPRLKEYERILKAEDKDNEFVLFIDEFHKVVSSFGSGNKIGGDSLKEPLANESLQM
                     ITATTRKEYENYIKRDDALGRRLQPLELNELKSDVVVDILRQWLNTSSVKDDPQASFR
                     LSQRIPDELLQEIVQANKLYREDLSEPAKSIAVMEDLIAKDRVDGNAYNRESLLQIFG
                     KYNIQLDSDFDMEKLQEELKSIKGQPLAVYTITMLFKRMKLGFDRFKGRKQPKATALF
                     TGSTGVGKTAMAKAVARGLYGDENKVLLVNMPDYKTKDDAMRFRRLIGTHVRHDPSAI
                     IVCDEYEKAGVETLDAFLSILDEGRISFTERGWDDREVHDTVSLSNTILFATTNAGAE
                     MFDSLNRYSSLSRVTNKENSNEYSTELKREYRKVASGIKQALIGEGLKPEVINRFGRI
                     VPFWALSEQTLLEIAYNEMLKSAELVRKHLNIDLRLPPRKERVYGQNFYADDLSMHLV
                     FDMASQEDSKSGGARNILHLVESEFEEAITEAIITYPNAKIFDVKLSEAGAFEGKGDS
                     NAEIIAKPSVAESKENKIVFSIGGA"
BASE COUNT         1434 a          564 c          775 g         1110 t
ORIGIN      
        1 tcttttcgtc tgataaatgg ataatattag ttattttatt gctaattagt cttttatgat
       61 gactgaaata gctaaaataa atatcttgtt gaataattgc tgatacttta agggatatct
      121 aaatttgctg attgatttat ttatgttatt atgatattat aataacataa atgtgttacg
      181 cccgaacctc attttaaaaa aatgaagggg tgagacaatg aatgctatta attggcatga
      241 atttgttaac tatatggaga gcttactaga tgatctaaaa agttttagta ttgctgatgc
      301 agtaaagcat gggttaccag ttagtacaac gatgaaatta cgaggattga aaattccaga
      361 tgaaattatt aagctgagaa ctaaaacaat tattgaaacc gcaaaagtcg ttgacaatat
      421 gaaccatgaa ctaaaaggca agaaaacaac ttacgagaaa gattttgaac gttttttaaa
      481 atctgttatt ggaaaatatc taggtaaaaa atctttcagt gaattgagag atgaagagat
      541 tgtacctact ccaatggtta gcaaaatccg aaaaaacaaa gctgagggga attactatct
      601 tggtacaata gcaaaaatca ttgattattt ggaaaataaa gagcaaaaga aaggaacgaa
      661 gaaaggaaga taatttttgt gttaaatatt aatggggaag aatataccga aatgattcaa
      721 aatcccgaac ctagaattac cgaaaaaacg tctgattgtt tgctctttta tgaaaggggg
      781 cagacaaact gatggaaaaa aacgtcaatt caacatctac caaagatcaa tacagtatta
      841 ttggagggtt gcttggtgat aaggataaga gaacacgtca cggattacgc attatgcttg
      901 caacttttct aggagtgctt tttttattaa caggcttggg tagctacatt tatcaattag
      961 gaaactataa ttattatctc gtgaacaata caacacctat agggtcttca ttggagtttg
     1021 ctcgatcagg agcagaaatc actatttcag atgtgtggac agatcaaaat agagatgtaa
     1081 cagtagtaaa acttggttac tcccaaaacg ctagaaagtt gttatctacg caaggaaaaa
     1141 actataactt atatatggca acaaaagaaa aagatcgtcc taagaagctt gaaatgagct
     1201 atgggatgtt aagtactgat ggggatgctt tcttattcct aaaaggtaaa ttagaggaaa
     1261 gagcttatca agtttttatt gcaaaccaag tatcactaat ttctggtgat gatacagctg
     1321 acgatgatac caaagtcaat gtgaatgaag cagattcagt tacaaaagca ctttctcaat
     1381 attctatgaa tgatgttaat aatgaaggag taatttcttt tgggaaaaaa gcaaaacctg
     1441 ttaatactcc tgataatatc aatttcagaa taaatccata ttcagattca actaatgtct
     1501 atcaaggaag ctttcttacc aaagatggcg atattgatta ttcaaaagtt gtagcagtta
     1561 caagtaagaa taatgttgta aataagctag aagatcagtt aaatgcttca aatactaaca
     1621 tcaagaaaat tgaagccaca aaagctgagt atctggaacg tttacaacaa aatcctgacg
     1681 ataaagattc gcaagaaagt attcgtcaac tagacagtcg aatggagacc gaaattaaag
     1741 caatcaatga tacacaaaaa ttgattgatc aatacaaaaa tgctgacttt aacaaagatt
     1801 cttttggaga catgaagacg acatttaagt acctaacttt gactgactag aagtaaatta
     1861 ttcttatttt agaggaagtg aacacaaaca gatggaaaat agtaccgagt tttttaagaa
     1921 tattcaaacc gaatttaatc atgctctcac accaatgaaa ccaccaaaac ataagttagt
     1981 gggacgtgat gacgaactcc atgagttgtc actgatcttg aatagaccgg aaacaccgca
     2041 agctatcttg ttaggtgaag ccggaacagg taaaacagca cttgcaagtg aatgggctga
     2101 aagacagcga caagatggaa agaaagtaca agtatataaa ttatcaatcg gacaattgcg
     2161 tgttggggga gatgatgtcc tccaaacacg tttagaaaca ttaattccaa gattgaaaga
     2221 atatgagcga attttaaaag ctgaggacaa agataatgaa tttgtcctct tcattgacga
     2281 gtttcataaa gttgttagct cttttgggag tggcaataaa attgggggtg attcgctaaa
     2341 agaaccgtta gcaaatgaat cacttcaaat gattaccgct actacaagaa aagaatatga
     2401 aaactatatc aaaagagatg atgccttagg gagacggcta caaccgttag aattgaatga
     2461 actaaaaagc gatgttgtcg tcgatatttt gagacaatgg ctaaatacat cctctgttaa
     2521 agatgatcct caggcttctt ttagattatc gcaaaggatt cctgatgaat tacttcaaga
     2581 aatcgtacaa gcaaacaaac tttatcgtga ggatttgtcc gaaccggcta aatcaattgc
     2641 tgtaatggag gatttgattg caaaagatcg agtagacgga aatgcttata atcgagaaag
     2701 tctattgcaa atatttggta agtataatat tcagctagat tctgattttg atatggaaaa
     2761 acttcaagag gaattaaagt ccataaaagg acaaccgtta gcagtttata ctataacaat
     2821 gttgtttaaa cgtatgaagc ttggatttga tcgttttaaa ggtcgaaagc aaccaaaagc
     2881 aactgctctg tttacaggat caacaggggt cggtaaaact gcaatggcta aagccgtggc
     2941 aagaggactt tatggcgatg aaaataaagt tctcttagtt aatatgccgg actacaaaac
     3001 caaagatgat gcaatgcgtt ttagacgact tataggtact catgtaaggc atgatccttc
     3061 tgcgattatt gtgtgtgatg aatatgagaa agctggtgta gaaacactcg atgccttttt
     3121 atcaatttta gatgaaggta ggataagttt tactgaaaga ggatgggatg atcgagaagt
     3181 tcacgacacc gtttcattgt caaatacaat tttatttgcg acaacaaatg caggtgcgga
     3241 gatgttcgat tcgttaaacc gatatagctc actatctcgt gttacaaaca aggaaaattc
     3301 taatgaatac agcactgaac taaaaagaga ataccgaaaa gtcgcatcag ggatcaaaca
     3361 agccttgatt ggtgaaggtc taaagcctga agttattaac cgatttggac gaatagttcc
     3421 attttgggct ttatccgaac aaactcttct ggaaattgct tataatgaaa tgttgaagag
     3481 tgctgaatta gtacgaaaac atttaaatat agatttacgt cttccaccta ggaaagaaag
     3541 agtatatggt caaaattttt atgcagacga tctctccatg cacttagttt ttgacatggc
     3601 ttcacaagag gattctaaat cagggggtgc aaggaatatc ttgcatttag ttgaatctga
     3661 gtttgaagaa gcgattactg aagcaataat tacttatcca aacgcaaaaa tattcgatgt
     3721 taagttgagt gaagccggag cgtttgaagg aaaaggtgat tctaatgctg aaattattgc
     3781 aaagccttcc gttgctgaaa gtaaagaaaa caagattgta ttttcgattg gaggggcgta
     3841 gctatgaaat tcaagaaata tttactacaa aaccttagaa taa
//
LOCUS       DARBEC010000143         3873 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.150, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000143 DARBEC010000000
VERSION     DARBEC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.150"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            156..2255
                     /locus_tag="SCL80_002688"
     CDS             156..2255
                     /locus_tag="SCL80_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEG1108519.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            2451..3314
                     /locus_tag="SCL80_002689"
     CDS             2451..3314
                     /locus_tag="SCL80_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEG1108520.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            3419..>3873
                     /locus_tag="SCL80_002690"
     CDS             3419..>3873
                     /locus_tag="SCL80_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEG1108521.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKE"
BASE COUNT         1542 a          646 c          781 g          904 t
ORIGIN      
        1 cttgagacta cgcagtctca agcttttttt tctataggaa ggaaattcat tcattatttt
       61 taagtaaatc ctaaaaataa gctattggcg tgacgtatag aatatgatag attaaatggt
      121 agcgtaaacc tagtaaagga gccgatcgct aagtgatgaa aaaatgtcca aattgcggat
      181 cgaaaaaaat ggagcagaat actatctgtc cagactgcgg tcatcaaata actgcagaag
      241 atcagcaaat aaaagaatca attgaagaaa caaatacaga tgcagctatg caggataaat
      301 caactgaaga tgcagtcttt cctgatacag aattaaacga tccaatcgaa tggtcggaat
      361 tgaaagattt acctctagaa tctgtaatgg aattattcga aaaagcagaa ccagaagacg
      421 aagaaccacc tgctcacgat caagtaacta atatagagaa aaatgaacag aagaaagcaa
      481 ctgttcatga gacttcggaa gaatcaggaa agaatcaaga agaaacgctt gaatcaaaac
      541 aaaaaagaag agtagctgaa ctgaaagaaa tcgtcgataa tgaggaagaa aattcgatac
      601 tatccgctta tatcaaagca catcgtgaag atacaaaaga agagcacgcg aaagagctgc
      661 tcaaaatgat cagtgaaaaa attgcacagg aaaacaaagt agagccagaa aaggaacaag
      721 aaactgaaaa agactctgat agagaaaacg ccacaggtaa agaaacagta aaaataaaag
      781 acgtaaaaac ggatatcaca gaaaaaactg aagtatttac tgaaaaacaa ttagaaaagg
      841 aaaaaacgaa taaactgccg gaagcagaga aaaaagaaat actcgaaaac acagacaaaa
      901 agtcaacaga gcccgatgaa ataaatagtg aatccgatgc tgaaaatcaa tctgtaagca
      961 aagagggtac tgatcttgaa gcaaaaatag aaaaagaaga gaaccagcct aagctgtttg
     1021 aagcagaagc gaaatcaata acaatcgtgc cagatactga aaaaacacat caagaatcaa
     1081 acttagaaaa aacagatcca gataaagcgc aaaagaaaaa atcaaaaaaa gtaccttatc
     1141 tcgttttagc agcagtactt ctactcggtg gaggaagctg ggcttattat gaccatcatc
     1201 aaaaagtaga agccgaagta gcagcagaag caaaaagaca gcaacaaatg gaagaacttc
     1261 atgagaaact tgctgccttt tatacagatg atcaccatca atttattcgt gcagatatga
     1321 ttcatcaaga tttatcaaaa ctaaaaaatg atttatccag tcttaagaat gaaaaaggat
     1381 attcagagtt agaaacaact taccaagata tccaatctaa gatccgagat atccaaaaag
     1441 tgaatgagtg gttcatagca cctgccatcg tcgatgatca tctagcagat gacatcaagt
     1501 tgaaagcaga taaagagatc caagcaatcg aaacggataa cacagatttt ggaaaattga
     1561 ttcaacaagc acaaacagaa gctaaaaatc aatatgctga attgcaaaca gctaaagaaa
     1621 aaacagctat agtctttaaa gacggaaaag tagtggattc agcaacacgc gaccaataca
     1681 agacggcaaa agaagcagta gaaaaagtga aaaatacaga tctatctgct cagttgaatg
     1741 aacaattgaa acaagtagat aagatgctca ctgataaaga gaaaaaagaa gaagaggaaa
     1801 agaaaaaagc agaagaagcg aaaaaagcag ctgaagaaca agcaaaacaa gccgctcaag
     1861 cagcagctgc agctcaagcc gctcaacaag ctgcggtcgc acaagcagca cagcaagaag
     1921 cggcatcctc gaccccttcc tcttcaaact cagcgaatca gccaatcaaa tctgccagag
     1981 caagtgatat tgccgatgca tctaatccag catggacgtg ggcaccaggt gtccaaagca
     2041 aagtgattgc tacatgtatc caacgtggat atatcgttga aggaggttac agattagaaa
     2101 aagccagaat cgaaaatggg gaaggctatt ataatctata tgccacttca acaaaatctg
     2161 cattgatgaa aggcattgga gaaagtgctt tgccatttta catcgtaacc atcaactgta
     2221 aaactggatg gttcggagga aatgggagca attaatttta ggcaaatgaa agaggctgtg
     2281 agcctgccgt ttaacaccgt ttattcgaaa aaggggagta tgacacaact gttgtcatac
     2341 tccttttttc acctttccaa accaaggctc agctattcaa gtagataaca ttatgttatg
     2401 atagcaaaga aatgtattaa gaaaaccttt cactatagag ggagaaaaaa atgaacgatt
     2461 tattagcaac tatatttaca ggtattatta ttgatgaaaa tgaaaaaaac tactttgtcc
     2521 aaaaaaacgg acaaaccttt ggcttaagca aatcagaagg tgaacacaaa atcggtgaag
     2581 cagttgaagg attcggttac caaaatcaaa aacatgaaaa tcggatcact acacaaattc
     2641 ctaaaagccg tatcggtcat tacgcttttg gaatcgttac tgctacacga aaagatttag
     2701 gtgcgtttgt cgatatcggt cttccagata aagactttgt cgtatctctt gatgaattgc
     2761 cgacaatgac agagctatgg cctaaaaaag gggatcgctt gatgatttcc cttagagttg
     2821 atgcaaaaga ccgtatttgg ggcagtttag cagatgaaaa aatattcaaa tctttaagca
     2881 aatatggaac agaagaaatg aaaaacaaag atgtgatagg aacagcatac cgcttgaaat
     2941 taacaggtac gtacgtttta acggaagatt tctatcttgg gtttatccat ccttctgaac
     3001 gatataatga acctcgtcta ggcgagcaga taaaaggtcg agtgattggt gtccgaccag
     3061 acggtgtact gaatctttca ttgaagccaa gagcatatga agctatatca gatgatgcac
     3121 aaatgatttt aaccatttta gaacgttctg cagatcagca aatcaacttt acagataaat
     3181 cggatccaga agaaatcatg aaagcttttg gtatcagcaa aggagcattt aagcgcgcat
     3241 taggtaatct tttgaaacaa ggattgatta cacaagaaaa cggtgtgaca aaactagtca
     3301 aaaaatctaa ttgagaatcc cttgcatgtt tttcttaact agcttataat aattttaatt
     3361 aacatagtgt tatatctttt tttatttgta attattataa attgttgggg ttgagtaaat
     3421 ggtcatgaat gctatttcgg cgatgaaaaa aacaaaacag cagctacatg aagcggggtt
     3481 taagctgaca ccgcaaagag aagctactct gcttgtgctt ttggaaaatg agaaagagca
     3541 tttatctgca gaagaaatct tctttttagt gaaaaaaaag agtccggaaa ttggacttgc
     3601 tactgtttat cgaacccttg aaatcttgac agaattgaaa attgtcgata aagtaagttt
     3661 taatgatgga ttagcccgtt atgatttgag aaaagaaggc gccaaacatt tccatcatca
     3721 tttactatgc ttgaactgcg gcacgatcga ggaagtagaa gaggatcttt tacttgaagt
     3781 ggagcaagta gtagaagaac gttatcattt tttagtaaaa gatcatcggc taacgttcca
     3841 tggaatctgt caggaatgct atgaaaaaga aaa
//
LOCUS       DARBEC010000144         3796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.151, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000144 DARBEC010000000
VERSION     DARBEC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.151"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..254)
                     /locus_tag="SCL80_002691"
     CDS             complement(<1..254)
                     /locus_tag="SCL80_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108522.1"
                     /translation="MMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFEFIYPIV
                     EATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDT"
     gene            complement(244..1443)
                     /locus_tag="SCL80_002692"
     CDS             complement(244..1443)
                     /locus_tag="SCL80_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002389929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1108523.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDIEGKVLSMYARGMSQCDIAATVEDIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKVGGVEDVFFISMDGVSGLKEGAKAIFPSVIVQRCIVHLVRNALRYLPSK
                     DYKEVCRDMKKFYSASSLNAAHAAFDSFQNRWSHYSGAVDVCKRNFSHVEQLFDYGSA
                     IRKIMYTTNAVESIHSSFRKVTKKGVFSNENAQTVDKVHFMDDVYSLFFYNRFMKTNA
                     RSDSYDV"
     gene            complement(1573..2949)
                     /locus_tag="SCL80_002693"
     CDS             complement(1573..2949)
                     /locus_tag="SCL80_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108524.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            complement(2949..3605)
                     /locus_tag="SCL80_002694"
     CDS             complement(2949..3605)
                     /locus_tag="SCL80_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEG1108525.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            complement(3615..>3796)
                     /locus_tag="SCL80_002695"
     CDS             complement(3615..>3796)
                     /locus_tag="SCL80_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108526.1"
                     /translation="SEDSMISGGSLINNSSFMSNITTLGQSYLILISIIVLSVVFASS
                     LMLIKKPKEILSKIS"
BASE COUNT         1176 a          708 c          523 g         1389 t
ORIGIN      
        1 gtatcgactt ccttaatggt ttgtcgcatg gaacggatac cgaataaata ctgaataaac
       61 accaatttga tcagaatcac tggatcgata cttgggcgac ctaaatcgga gtaagtggct
      121 tcgacgattg gataaataaa ttcaaaatca agcgcggcat caatctttcg aacgagatga
      181 tcttgaggaa ctagatcatc aatcgtaagc atcgcaaatt gatttctacc attcgtattg
      241 tttttagaca tcatagctat cactccttgc attcgttttc ataaatctat tataaaagaa
      301 aagactgtag acatcatcca taaaatggac tttgtctaca gtctgcgcat tctcattaga
      361 gaacactccc ttttttgtga cttttctgaa gctagagtga atactttcaa ctgcattagt
      421 agtgtacatg atttttctaa tggcgctgcc ataatcaaag agttgttcaa catgagaaaa
      481 gtttcgtttg catacatcta ctgcaccaga ataatgagac caccgatttt gaaaactgtc
      541 gaaggcagcg tgtgcagcgt ttaaagaaga agcactgtag aattttttca tgtctcgaca
      601 gacttcctta taatccttac ttgggaggta tcgtaacgca tttcgaacaa gatgaacaat
      661 acagcgctgc acaatgacag aaggaaaaat tgcttttgcg ccttctttga gaccggaaac
      721 accgtccatc gaaatgaaaa aaacatcttc tacaccacct actttcagtt catcaaatac
      781 ttgcatccag cgatttttag attctgtttg attgagccat aggcctaaaa tctccttgtt
      841 tcctttaaga tcatagccaa gaatggtgta cacagcatat tctttagctt catagttttc
      901 gcgtaaagtg acgtacatac aatcaacgaa tagaaatgca taacacttgg ctaatggccg
      961 agcttgccat tcttccaatt caggaaggac ggcatccgtg atatcagaaa tcatttcgtg
     1021 ggaaatatca aagccgtaaa tatcttcaac ggttgcggcg atatcgcatt gactcattcc
     1081 tcgtgcatac atggaaagaa ccttaccttc gatgtcggag acatctcgtt ttctattagg
     1141 aatcaactca ggttcaaaag aagcctcccg gtctctggga acatcaatgt ttactttacc
     1201 aaagctcgtt ttaagcgtct tattgccata accgtttcgt cgattgtcat gtttctttgg
     1261 ctctttagaa tgggcgtcat accctaaatg gttattcaat tctccttgaa gcattttttc
     1321 aaataaggga ccaaacacat cttttaaaac atcttgcata tcctctacag attcgggttg
     1381 ataagtattc aaaatggatt cagctaactt ctcagcatca ggatttcttt tctttctagc
     1441 catcgtgtaa tcacctcttg atcttatttt agaaaaataa aaaccgtgaa gcaaccaccc
     1501 gcctaggatt gatggttact tacacggttt atattacact ctcatttttg gtgctaaaaa
     1561 gcaccgaaaa ttttaattat tgtcaattaa taattctttt ggattccttc ttaatatatt
     1621 gaatgaagaa ataatcaatg atacaagaag tactaaactc ataaatataa ctacataaat
     1681 catgaattta ggtaatacat tgatatctaa acttgataat gtcttgttaa atccttcagc
     1741 ttctgcccca ccacctaatt ggctagatgc agattgttta gctatttgtt tagctatatc
     1801 accagtaacc ttattcaata tattattccc aagcttgtca gctgtatatt gagctaagaa
     1861 ataggaacca acgaatgcgg ggattgatat aaataccatc tcaattagga attgaccaaa
     1921 aatttctaat tttgatatac ctaatgatag taatactgct atttctttct ttctagcatt
     1981 catccataag aataataata atgaaactac aactcctgca aatattaatg agccaaaaaa
     2041 taatttattt gaaattgagt aaatacctga tatagattgt tgtaatgcag ggtaatttga
     2101 tgaactttta atcaaattat attctctcca atttatatct aatttcccaa gatcttttat
     2161 tacactatca agattcttat caccttttac aaagaatgtt gcatcttggt atacagctgt
     2221 atcttcggta ttaccataaa ctttagcagc tgtatgaata tcagtgatta atgtattttc
     2281 gtatagttct tgtgctgcac ttactccacc gctattatgc ccatcgaata gacctttaat
     2341 ttctacttct actgtttcat ctgcaccttt ctcattatca gcatcaaata aattagattt
     2401 tatttttatc ttatctccaa ctttaaggtt atttttttca gctaaatctt tatgcattaa
     2461 gattttattt ttatcttcat tttctaaatg ctttccttct actaatttat atgcttctga
     2521 tacaaatttt gtttcttttg ttgagtcgtt aaccccagtt aacataactg ttcttttaaa
     2581 attctttgct ctttcaggtg attgattcgc aagagtctct tgagtttcaa taatatcata
     2641 atcaactaaa tctgcaacac tgtttattct ttttacatag gagtctatac tatctgtttg
     2701 agatatcttt ttaatatctt caccttttac atttccccca cctctaggtg ttcctggatt
     2761 tacttgtcta tttatctcca tagaaaaact atttgttata ttagcaaatg tttcttttga
     2821 agctctgtcc gtagcatctt taatagataa actaatgaga cttaaagttg acatagataa
     2881 aataactaat atgataatta atgattttaa actttttcta gttacataag caaatgcatt
     2941 ttttatcatt attcaacctc caaattcatt ttatttactt ttttcaactt tttaccactt
     3001 agttctaaaa tgatatccgc agaatctgct acttccttac tatgtgttac aactattaca
     3061 catttatttc tatctttagc taatgtcttt aatatattaa ttatttctcc agcagtaaca
     3121 ctgtctagat taccagtagg ctcatcagct agtattattg gagcatctga taccaatgct
     3181 ctagcaatag ctactctttg ttgctgtcca ccagataatt tcataacatt tctttttatt
     3241 tgttttttat ctaaacctag ttcaaacaag atactttcat ctgctgattt atttactaat
     3301 ctaatatttt caattggtga taaataatct attaaattat agttttgaaa tactaaagat
     3361 atattatttt ttctatgatt actatatcct ttattttgta tatcttcatt cttaaacaat
     3421 atttttcctg tttgaggttt atcaagtcca gcaagtaagg aaagaagtgt agattttcct
     3481 gttcctgact ttcctactat cgcataaaac ttcccaagtt caaatttttg atttactcct
     3541 gacaaaactt tttctttaga atttgcataa ctatatgcta cattctttat ttctaatata
     3601 tccattattt tctcctaact tatttttgat aagatttctt taggcttctt gattaacatt
     3661 aatgaagaag caaatacaac tgataaaaca ataatactta ttaatattaa ataactttgt
     3721 ccaagtgttg ttatatttga cataaaacta ctattattta ttaaacttcc accggaaatc
     3781 attgaatcct ctgagt
//
LOCUS       DARBEC010000145         3753 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.152, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000145 DARBEC010000000
VERSION     DARBEC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3753)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3753)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3753
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.152"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(202..2199)
                     /gene="tkt"
                     /locus_tag="SCL80_002696"
     CDS             complement(202..2199)
                     /gene="tkt"
                     /locus_tag="SCL80_002696"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEG1108527.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2301..2552)
                     /locus_tag="SCL80_002697"
     CDS             complement(2301..2552)
                     /locus_tag="SCL80_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEG1108528.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2699..3322
                     /gene="lexA"
                     /locus_tag="SCL80_002698"
     CDS             2699..3322
                     /gene="lexA"
                     /locus_tag="SCL80_002698"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEG1108529.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3502..3720
                     /locus_tag="SCL80_002699"
     CDS             3502..3720
                     /locus_tag="SCL80_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108530.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
BASE COUNT         1039 a          801 c          694 g         1219 t
ORIGIN      
        1 aatggaaagt tttgtctcgc ttaatttgac aaataatatt ctcaagaaac acaaactaat
       61 ccgaagaatt ctttgttttg tcatttaaat gtaacatagc cgaaaacgaa aagtaatatt
      121 gatttattct tctaacacat aaagaacagc tgtctccaac ttgcagacag ctgttcttat
      181 cagtcaaaca attgcttgga tctatgatag ttgattgtac gtattcacga cattttctac
      241 tgtaaatcca aattcttcta aaattttagt gccaggggca gatgcaccga aatgatcgat
      301 tgtaatcgtt gcgccctctg ttcctacgta gcgttcccat ccaaatgatg cagcagcttc
      361 gatagccaca cgtttcttca ctgatttagg aagtacactt tctttatatt cagaagattg
      421 tttttcaaag aggtcaaagc ttggcataga aacaacagaa acatctttgc ctttttcagc
      481 caattctttt tgagctttca cagccaaatc aacttcagaa cctgttgcaa tcaaaatacc
      541 ttctggtgtt tctccttgtg atggagaaag aacatacgcg ccttttttca ccatttcatc
      601 tgctacttct tttgtagaag gcaatactgg aaggttttga cgactcaata caaggatagt
      661 aggcgcatct gttgtctcca tcgctacttt ccaagcagca cgtgtttcat ttccgtctgc
      721 cggacgaatc acttgaacac caggcatgca gcgaacagat gctagttgtt caatcggttc
      781 gtgcgttggt ccgtcttctc cgacagcaac cgagtcatgt gttaagacgt aaacaacagg
      841 agtatgttgg attgctgcta aacgaactgc tggtcttaga taatcgacaa agacaaagaa
      901 tgttccaccg tagatacgag ttccgccatg aagttggata ccattcatag ccgaagccat
      961 tgcaaattcg cggacaccga accagatgtt gcgtccttca taatgttcag gagtgaaatc
     1021 tttatctgca gccaccattg tattattgga acctgacaag tccgctgaac caccccagaa
     1081 gcttggtata gctttcgata attcttggat gacttcttta cttgaaacac ggcttgcttg
     1141 actgcttcca acttcataag ttggcaattc tgcatcccag ttttctggta gtttgccatc
     1201 aaaagcatct tcaaattgtt gtgctaattc aggataagct tttttataat tggcaaacat
     1261 ttcattccat gcatcttcag ctttttgtcc ttcttctatc atcgtttgat ggaagcgggc
     1321 agctacttct tctggaacag tgaaatctgg atactcccaa ccataaactt ctttagccat
     1381 cttgatgcca tcttcaccaa gaggagcacc atgaacagca gatgttcctt ctttaggagc
     1441 accatagccg atgactgttt ttacttcgat caatgttggt ttgtctgttt cagcttttgc
     1501 ttcttcaatc gctttagaga ttgcttctag gtcatttccg tctttcacta agatatgttg
     1561 ccagccatat gcttcatagc gagcaccaac gttttctgtg aaagcttttg aagttggacc
     1621 gtctagtgaa atatcgtttg aatcgtataa aacgatcaat ttccctagtt tcatatgacc
     1681 tgccatcgaa cttgcttctt gagatacccc ttccatcaag tctccgtcac cgcatattgc
     1741 atacgtatag tgatccatca cattaaagtt ttctttgtta tacgttgctg caaggtgcgc
     1801 ttctgccatt gccataccga cagccattgc aatcccttgt cctaatggcc ctgtagtggc
     1861 ttctacacca tctgtatgat gtacttcagg atgtcctggt gttttagaat cccattgacg
     1921 gaattgtttc aaatcatcga ttgtcacttg atagccagct aaatgaagca agctatacaa
     1981 cattgctgac ccatgtcctg ccgaaagaac gaaacggtcg cggtcagccc agttttttga
     2041 tgtcgttgga tttactttca ggtgttttgt ccataacgca tatgccatag gtgctgcgcc
     2101 catcggcaat ccaggatgtc ctgagtttgc tttttggact gcttcaatac ttaatgtacg
     2161 aattgtgtta acgccaagtt ggtcaatttt gtcaaacaaa ataacccctc ctattaattt
     2221 aggttttttt tacctttatt tcttacactt tcattctata agatgtcaca cgaaaaagca
     2281 atctttttca gtatttttta ctaaaagttg tcatttcggt tgtgtaagcc ttttttcttt
     2341 tggatttctt ttaatttttc cggggtcacg tcattgccct caggatcgac gactttcatt
     2401 ccttcgatat gatgacgcat accggaacga aaagcttcca aatactcttt gcgcaagttg
     2461 atttgttctt gttcttcggc ttttgagaga ccattttcct tctttttacg tgcaagttca
     2521 ttgatccgct ctattttttc aggtgacagc atgctcagcc ctccttttac tctccttcat
     2581 attatataaa aactgaaaaa aaaacaacta gataaactaa ataaaatacg aacattcgtt
     2641 tgatttagcg ctttttatat ggtactattt atttatcaag tgaaagaagg tgtaccttgt
     2701 ggcccgacaa actgaatcaa gacaaatcga agtcttacga tatatccatg aacaagtaac
     2761 ccaaaaagga tacccgccga ctgtccgtga aatcggggaa gctgttcagc tttcttctac
     2821 ttctactgtt cacggtcatc ttgcacggct tgaaaaaaaa gggtttatcc aaagagatcc
     2881 gacgaaacct cgtgcaatcg agttgacaaa acaaggttta gaaaagattg ggattcgtcc
     2941 aacaactatt ccagtacttg gcgtcgtaac agctggagaa cccattttag cagtagagga
     3001 tgcttcagac ttctttccaa tccctcctaa tctttcttcc gaagaaggca gtctctttat
     3061 gctgacaatt cgtggagata gtatgatcaa tgctgggata ttggatggtg acaacgtgat
     3121 cgttcggaag caagaaactg ctcaaaatgg cgatattgtc attgcaatga ctgccgagga
     3181 cgaagcgact tgcaaacgat tctataaaga aaaaaacttt ttccgtctgc agcctgaaaa
     3241 cgacttgctc gaaccaatca tcttagatca agtatctata ttaggacgag tagtaggact
     3301 ttatcgtagt catatctatt aattgctact aattaaaaga aaacttttta aaaagagttt
     3361 gagacgttgg tctcaaactc ttttttcgcc cgggttcttc ttctttcaaa tttttatcac
     3421 ttccctcttt acaaaccgaa caaatattcg tatactaaat atacgaacaa ttgttcgcat
     3481 aaattaaaga ggtgagaatt catgaaatca atgagacgtc ttggcttagt attaactgtt
     3541 ttgtttttag gtattattat tggtaatttc ggcatacgtc cagctttact tgtgtttgtt
     3601 ccgttattta ctttgtggtt catgttgtgg gatgaaaaga agtaccgtca atctgagcgg
     3661 cgcagaattc atgaagatca tttctatcat gagccctact acagatccag gcaatcataa
     3721 taaggattga ctgctaatta ttcaaaagac aag
//
LOCUS       DARBEC010000146         3718 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.153, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000146 DARBEC010000000
VERSION     DARBEC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3718
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.153"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            102..2543
                     /locus_tag="SCL80_002700"
     CDS             102..2543
                     /locus_tag="SCL80_002700"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEG1108531.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
     gene            complement(2873..3577)
                     /locus_tag="SCL80_002701"
     CDS             complement(2873..3577)
                     /locus_tag="SCL80_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108532.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         1193 a          654 c          813 g         1058 t
ORIGIN      
        1 tttaaggatt gttaaaattt aatcttagaa aaaaataact ggaacaagag ctatttgttt
       61 ttacagcaaa gaatggtata tttttgtagg aggtggctta cttgaaagaa gtcgttatga
      121 tagatgctgc aagaacgccg ataggaaagt atcgcggaag tttaagtcca tttactgcag
      181 ttgaattagg cacacttgtg actaaaggat tattagataa aacaaaactg aaaaaagata
      241 aaatcgatca agtaatattt ggaaatgtcc tgcaagctgg taatggtcaa aatgttgcca
      301 gacagattgc tttgaacagc ggtctccctg tagatgtccc agcaatgacg atcaatgaag
      361 tttgcggatc tggtatgaaa gcagtgatct tagcacgcca gctgatccaa ttaggtgaag
      421 cagaacttgt gatagccggc ggaacagaga gtatgtctca agcacctatg ctgaaacagt
      481 atcagtcaga aacaaatgaa tatggtgaac caatttccag tatggtcaac gacggattga
      541 ctgacgcatt ttcaaatgca catatgggat taaccgcaga gaatgttgca acacaatttt
      601 ctgtgagcag agaagagcag gatcgctatg ccttgtcgtc ccagttgaaa gcagcacatg
      661 ctgtcgaagc cggtgtattt tctgaggaga tcatcccagt caagatttct gatgaagacg
      721 tgttatctga ggatgaagca gttcgtggaa atagtacatt ggaaaaactg ggcacgttac
      781 gtacagtatt ctcagaagaa ggaactgtaa cagcaggaaa tgcttccccg ttgaatgacg
      841 gtgcctctgt ggtgatcctt gcatccaaag aatacgcaga aaataataat ctgccttatt
      901 tagcaaccat caaagaagta gcggaggtcg gtattgaccc aagtatcatg ggaatcgctc
      961 caatcaaagc cattcaaaaa ttgacagata gatcaggaat gaatttatca acaatcgatc
     1021 tttttgagat caatgaagca tttgcagctt cttctatcgt agttagccaa gaactgcagt
     1081 tagatgaaga aaaggtcaat atatatggag gagcaatcgc attaggccat ccaattggtg
     1141 caagcggtgc acgtatcttg actacattag cttacggatt gcttcgtgaa cagaagagat
     1201 acggtattgc ttcattatgt attggtggag gtttaggctt ggcagtcttg ttagaagcga
     1261 atatggagca aacacataag gatgtgcaaa aaaaaaagtt ttatcagctt acgccatctg
     1321 aacggcgcag tcaattaata gaaaaaaatg tattgacaca agaaactgcg ttgatttttc
     1381 aggaacaaac actttctgaa gagctctccg atcacatgat cgaaaaccaa gtgagtgaag
     1441 tggaaatccc aatggggatc gcgcagaatt ttcaaatcaa tggcaagaaa aaatggattc
     1501 cgatggcaac agaggaacca tctgtcattg cagctgcaag taatggtgca aaaatctgtg
     1561 gaaatatctg cgcagaaaca cctcagcggc tgatgcgagg gcaaatcgtt ctatcaggaa
     1621 aatcggagta tcaagcagtg atcaatgcag ttaatcatcg aaaagaagag ctgattcttt
     1681 gtgcaaatga aagttatcct tcaatcgtga aacgcggggg cggtgttcaa gatatctcta
     1741 ctcgggaatt catggggagt ttccatgcat acttatctat tgatttttta gtggatgtaa
     1801 aagatgcaat gggcgctaac atgatcaact cgattcttga gagtgtggca aacaagttac
     1861 gggagtggtt ccctgaagaa gagattctat tcagtatttt aagtaatttt gctacagaat
     1921 cattggcttc tgcctgttgt gaaatcccat tcgaacgctt agggagaaac aaagaaatcg
     1981 gtgaacaaat tgccaaaaaa atccaacagg caggggaata tgcgaagctt gacccttacc
     2041 gtgcagcaac gcacaacaaa gggatcatga atggtataga agctgtagtg gcagctacag
     2101 gaaatgatac acgtgcggtt agcgcttcga tccacgccta cgcggcaaga aatggattat
     2161 accaaggatt aacagactgg cagatcaaag gggataaact agttggaaaa ctaacggtgc
     2221 ctttggctgt tgccacagta ggcggagctt ccaatatcct gccaaaagca aaagcatctt
     2281 tagctatgtt agatattgat tcagccaagg aactagcaca agtaattgct gcagtagggt
     2341 tagctcaaaa cctggctgct ttgcgtgcat tagttacaga aggaatccaa aaaggccaca
     2401 tgggacttca agctcgttca ttagcgatct caattggggc tattggtgag gaaatcgagc
     2461 aagttgcgaa aaaattacgt gaagcagaaa agatgaacca gcagacagct atacaaatat
     2521 tggaaaaaat ccgggaaaaa taaaaaagca atatctttag aagaataaag caagatttac
     2581 agttatttta tttactgcag atcttgcttt tttctctatc agaaaatatg tttattatta
     2641 gaatttttta tagttaaggt taggcatctt aagaaatcag gcaactccaa gatatcttca
     2701 ggtagaagag aagtcgccta acattgtagg atcataggtg atttgttgga caggtcaagc
     2761 tgatatttga gcctgatgtt agaaggaatt tctttttact tgctgtagaa attattatat
     2821 gactaaaaag gctgggattt taaatcccag ccttttatga caaatagaat ttttatcgat
     2881 cagtaatcgc tgtcgctgca aattgttcga tcaaaggaat caaagtcaat ttgactttgt
     2941 atttccgttc gagtgctttt tcgataacga actcattttc agtagtcata gaaatagttg
     3001 ctaatttacg aggttcatca gcattttttt cattaataac tacttcatgt tcattttgtt
     3061 ctgtgtaaaa tttaggatac tcagcaacgg cttttgaaaa atcagcaaaa cttaaagcgg
     3121 cattcgtatc agttgagcgt gtattttcag ttgtgtccgc ttttggtttt tccgtttttt
     3181 cttctggcac actgactgtt tcttctgtaa ctggtgtcgc aacagcttgc gcagttggtt
     3241 catcgttttt caatcgttga tcattccagt aagacacgat ttcaataaga agattgtcac
     3301 ggaatcgttc gaaaatcccg ttttctcctg aacgaattct ctttaataca gtattgaagt
     3361 tgtcatcgat tggcacgttg atccgtgtat cttcatttgt tttcccagct aatccgatcg
     3421 ttaagatttg acggtttttg ggatcaagca tgacttgtaa cggcaaacca taacgtcgca
     3481 agctctcttc ttctagagaa cggaataatt gtcctcttac accatccaat gaaggacgga
     3541 attgatttaa taatttttgt ttctgttttt tcttcatatg ttctctcctt cgctcatacc
     3601 ctttcgatta taagaaaaaa acagccaaat gaaaaggagt aatgcaaaaa aacttgcgta
     3661 tacgcaaatt acaacagaaa aatttgcgta ttttcccgtt ttttgaaaga aaaataaa
//
LOCUS       DARBEC010000147         3600 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.154, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000147 DARBEC010000000
VERSION     DARBEC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.154"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1125
                     /locus_tag="SCL80_002702"
     CDS             <1..1125
                     /locus_tag="SCL80_002702"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1108533.1"
                     /translation="IKKLNDVDVLEHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKKLLEAISSLQTTPENEAWLQGALEVFKHEQ"
     gene            1115..2332
                     /locus_tag="SCL80_002703"
     CDS             1115..2332
                     /locus_tag="SCL80_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1108534.1"
                     /translation="MSNDTQPEIRFPGFTDDWEQRKLGDFVVDYVEKTSVQNQFPMLT
                     SSQQKGIVLQEDYFANRQVTTENNIGYFVLPRGYFTFRSRSDNDVFVFNRNDIIDRGI
                     ISYFYPVFTLKSADSDFFLRRINNGIQRQLSIQAEGTGQHVLSLKKFKNIVAMFPSEE
                     EQQKIGTFFKQLDDTIALHQRKLDLLKETKKGFLQKMFPENGAKVPEIRFPGFTEDWE
                     ERKLGELGKTQSGIGFPDAEQGGSEGTPFFKVSDMNNIGNEYEMRNANHYVSNEQIER
                     KKWKPIKDVPAIIFAKVGAAIMLNRKRLVTSPFLIDNNTMAYLFNNTWDIYFGKILFE
                     TINLPRYSQVGALPSYNSSDIENISVKVPVKDEQQKIGTFFKQLDDTIALHQRKLDLL
                     KETKKSFLQKMFV"
     gene            2345..>3600
                     /locus_tag="SCL80_002704"
     CDS             2345..>3600
                     /locus_tag="SCL80_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1108535.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDSQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDGVYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNEKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT         1239 a          614 c          756 g          991 t
ORIGIN      
        1 atcaagaaat tgaatgatgt agatgtttta gaacacgatg gggacgtgat cggggatgcg
       61 tatgagtttc tgatcagtca atttgcttca gaagcaggga agaaagccgg agaattttac
      121 acacctcata tggtttctga tatgatggcg caaatcgtaa cgctcgatca aaaggaacga
      181 cgattcttta gtgtttttga tccaacgatg ggttcaggtt cattgatgtt aaacgtacgt
      241 aactacttaa cacacccaga taacgttaaa tatcatgggc aagaactaaa tacaacgaca
      301 tacaatctag caaaaatgaa cttgatttta catggtgtcg acgccgaaga aatgaatctc
      361 cgcaatgggg atacgttgaa taaggactgg ccaacagatg aaccttacac atttgatgca
      421 gtagtaatga acccacctta ttcggctaat tggtcagcag atacgacttt cttagatgat
      481 tctcggttta atcgttatgg gaaattagcg ccaaaatcca aagcagattt tgcgtttctt
      541 cttcatgggt tctatcattt gaaggaaaca ggtaccatgg ccatcgtctt accacacgga
      601 gttttattcc gtggcgctgc agaaggggtc atccgtcaaa aattactaga agatggcagt
      661 atttatgctg tgatcggtat gcctgcgaac ttgttctttg ggacatcgat tccaacgacg
      721 gtgattgtgt tgaagaagaa tcgtcaaact cgagatgtat tattcattga tgctagtcgt
      781 gaatttgtta aagggaaaaa tcaaaataaa ctatcggaag aaaacattca aaaaatcctc
      841 gaaacctacg ctgaaagaaa agatgtcgag aaatacgctc atttggcaac ctttgatgaa
      901 atcaaagaaa atgactataa cttaaatatc ccacgctatg tagatacttt cgaagaagaa
      961 gaaccaattg atatggttca tgtcggaaat gacatcaaga agatacgaca agaacagcaa
     1021 gtgctagaaa aaaaactttt agaagctatc tcttccctgc aaacaacacc tgaaaatgaa
     1081 gcgtggttac aaggtgcgct ggaggtgttc aaacatgagc aatgatacac aaccagaaat
     1141 ccgtttccca ggatttacag atgattggga acagaggaag ttgggggact tcgttgtaga
     1201 ttatgttgaa aagacaagtg tacaaaatca atttcctatg ctaacttcat ctcaacaaaa
     1261 agggatagtt ttgcaagaag actattttgc aaatcggcaa gtaactacag aaaataatat
     1321 tggttacttt gtactaccta gaggatattt cacgttccgt agtcgaagtg ataatgatgt
     1381 gtttgtgttt aaccgtaatg acattattga tagagggatt ataagctatt tttatcctgt
     1441 atttacttta aaatctgctg attccgactt cttcttaaga cgtattaaca atggaatcca
     1501 acgacaatta tctattcaag cagagggaac aggtcaacat gttctgtctc taaaaaagtt
     1561 taaaaatata gtagcaatgt ttcctagtga ggaagaacaa caaaaaatcg gcaccttctt
     1621 caaacaactt gacgacacga tcgctcttca tcagcgtaag ttagatttgc tgaaggaaac
     1681 gaaaaaagga ttcttacaaa aaatgttccc tgaaaacgga gcaaaagtgc cagaaatccg
     1741 gtttcctggt tttacggaag attgggaaga gcgtaagttg ggagaattag gtaaaactca
     1801 atcgggaatt ggatttcctg atgccgagca aggtggttct gaaggtactc cattttttaa
     1861 agtttccgac atgaataaca ttggtaatga gtatgaaatg agaaatgcaa atcattatgt
     1921 atctaatgag caaattgagc gtaaaaaatg gaagccaata aaagatgtgc cagcgattat
     1981 atttgctaaa gttggcgcag caataatgct taatagaaaa cgtttggtca caagcccttt
     2041 cttaatagat aacaatacca tggcttattt atttaataat acttgggata tctattttgg
     2101 taaaatattg tttgaaacta taaatttgcc aagatactcc caagtagggg cattgccttc
     2161 ttataacagt tctgatattg agaatatcag tgttaaggtt cctgtaaagg atgaacaaca
     2221 aaaaatcggt actttcttca aacagctaga tgatacgatc gctcttcatc aacgtaagtt
     2281 agatttacta aaagaaacga aaaaaagctt cttacaaaag atgtttgtct aggagggaag
     2341 aagaatgaaa attagtcacc gtgaggaagc agaagtcgaa gaacagttga ttcgtgtact
     2401 tggagaaggg cataatcagt ggacgtatcg tccggatcta aaatcagaag aagacctttg
     2461 ggtcaattta cgccaaaaaa ttatctcaaa taatcaagca gaactgaatg attttccttt
     2521 gaccgataaa gaatttgaaa cgatcaaaac ggaattactg ctgcgaacga aaaccccatt
     2581 tgatgctgcg aaatggttaa aaggtgaaaa tgggatggct cggatcacga ttgaacggga
     2641 agattcgcaa ttgggttctg tctcacttat cctttattcc aatcaagaca ttggtggagg
     2701 aatctctact tatgaagtgg tccatcaaat tgcaaaacgt ggaagtaaca tagaagcacg
     2761 ggatcgacgt tttgacgtca ccttattaat caatggatta ccgattgtcc aaatcgagct
     2821 gaaacaagta actgcaaaag atggtgttta tcaggcattt aaccaaatca aaaaatatgc
     2881 agaagaaggt atgtttcgga acaatatctt ttcgacctta cagttgtttg tggtttccaa
     2941 tgaacaaacc actcggtatt ttgcaaatgc cttaccgaaa gacttgcacc ctaagttttt
     3001 atttagttgg cgaacgaaag acaatgaaaa agtagaaaac ttatatgagt tttgtaaaca
     3061 agtgttaaat atcccggatg ctcatcgttt gattgcggat tatacaatcg tcagtgaaga
     3121 tcaagacaat aaaaccctca tggtgttaca tccttatcaa gtacatgcga ttcaagcctt
     3181 gtttattgcc gcaaataaac atcaatcagg gtatgtgtgg catgcgactg gttcaggaaa
     3241 gacgttgact agttttgtat ctactaagct tttagcacga aaatcgggga tcgatcgaac
     3301 gatcatgtta gtggaccgca aagatttaga taatcaaacc accacagagt ttacaaaatt
     3361 tgcttcggaa tttaatactg ggatttcttc cgggaacgcc aaagcgaata gcttgatcgt
     3421 tggaacggga agtgccaaag aattaagtga aaccctgttg gcagatgcca atgcaaatgt
     3481 tgtaatcatt acaacccgtc aaaaactaga tgcggcgttg aaatatgcaa agaaacaaga
     3541 agagaaaaaa ggaaccaatc gcttccaaaa attaatgggc caacatatcg tgtttgtagt
//
LOCUS       DARBEC010000148         3563 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.155, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000148 DARBEC010000000
VERSION     DARBEC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.155"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..2303)
                     /locus_tag="SCL80_002705"
     CDS             complement(<1..2303)
                     /locus_tag="SCL80_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108536.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTTGEDGKLAIDGLAPGAYQLVET"
     gene            complement(2975..3243)
                     /locus_tag="SCL80_002706"
                     /pseudo
     CDS             complement(2975..3243)
                     /locus_tag="SCL80_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
BASE COUNT         1000 a          792 c          560 g         1211 t
ORIGIN      
        1 gtttctacca gctgatatgc tcctggtgct aaaccgtcaa ttgccagttt tccatcttct
       61 cctgtagtta aaccggtttg tagcgtttct ccttctcggt tctgtaactc aaaaacagct
      121 ccttgaagga cttcaccact gccttcagca tgtttaatta gttcaacacc acccgtatca
      181 aggatgtttt caatggttaa cgtcacttct tctatggagt attcatttat aaaaacatca
      241 tgtaccgtcg catctaaaag atacccttcc ggtgcttctg tttctcggac ttgatagcta
      301 cctacaggaa gcggttcact taaaaattct ccgttttctg ccgttatacc ttcttgtatc
      361 acttgtgaat cgcttgataa aatttcaaat tttgctcctg ccagcctttg gccattctca
      421 tcagttttaa ttactttaat acgcccatcg attttccttg ttcttacagt actcgtattc
      481 aatcgattgt cactattcac aagcgttgca aagtttaaaa tttgatcgcc gaccgcatac
      541 gcctctacat tgaccttaaa agagagggta aaacttgtcg gaccaccaat cgtattattc
      601 gcactgaaac gaactattcc attattgact tcggcatcaa tgaaagaggg aatatctgat
      661 agaaatgaca caaattccat gccttcttcc aaaacatctt caataacaag cgtatttctt
      721 gttccagaaa acatcgagac atgcagagta tacgttagtt cattttcatt gagattgata
      781 acatttttgt ccacactttt tcttaaagag attgccggaa gattactttc agggattctc
      841 aagcggtagt ctcggctcat tacctgatta ttgtaggtca tactcgctcg attccgagaa
      901 aacccatcct cttgtcttcc ttcattgaac aagtcgacat cggtcacttc aagagcatat
      961 tcaataaaat acgattgacc ctctgctaga ttaccaaagt ccacaacaag tgtattcgtt
     1021 tccttattaa aagatacttt gtccacaaat tcctcggaaa cataatgagt tctaacgttt
     1081 gcttcacctg gattcgttac ttctgcgatc cgaaccccac ctgaaatagg ttctctattt
     1141 ccagtggaac tccatgtagt cggattcggt gccaaagttg ttccttgcgg tagtacatcg
     1201 gtaaccacta caccgtcttt tcgtgatcct tcataattta cctgcaaaat aaatttatta
     1261 ctgttggtca cgtcttgccc gtcaatcagc agattcccaa gatttccttg gctgtcgatt
     1321 tctctagagc catagtataa tttcgaaaaa atgggtcttg atgaaggaat aggcgtaatt
     1381 gaaaattccg tgtttgctgc tcgctcagca taagtaagac tcacttgaaa gtcattattt
     1441 ggctcagtac cgcctgattg aaagaaagca acttcaaacg tgatactgaa tgtatacgac
     1501 tgagcggctt catttaatcc ttcataatca aaggtaaatg gaaaaacaac gtctgtgcct
     1561 tcttggtaaa atgccagtgg ctttaaaggt gaatcatcag cgacctccac tctaatgaac
     1621 tcaattaagc caatcgatgc tctaggaact cgtacgacca tctccccata ttcttcaaga
     1681 atatcgccag cgcctccttg aatgtatccc tttaacgttt gtgtatgcct gctcggcaaa
     1741 gtggggcgct cagcagagat gccgatgtca taattcaaga gctcctcttg tctttcaatc
     1801 tgagattcct gcgttataga tggctcagat gatctggctt cattaaaaga ttcacttaag
     1861 ctttcttctg gtatattaaa agattcactt gtgctttctt ctggtacatt aaaagactca
     1921 cttgtgcttt cttctggtac actgatttct attgagaccg tatttgattg aatatcaccg
     1981 cttcttgcgg tcaattcgta tttacctgaa tgctgggaag aataagtaat cagacgttcg
     2041 taatcattga gttgcttcac ttccccttca atcaaatcct cactcttatc aatgaccaat
     2101 gcatttagca ggtctggtgt tcctctgact cgcaactgga actgctcacc caaagcagcc
     2161 tcctcttgat caacttcgat cgtcagcgaa cgctctggct gttctgctaa agactcagct
     2221 agaaccgtta tgttcccaaa ttgattgatc acaataagca taactagaaa catacttaac
     2281 agtttatgaa atttgttcct cactattact cccccttata attttgacta aacaacaagg
     2341 tacccgataa ttttctaaaa agattcccca actcaaaatg gctgcgttac ctccctcgta
     2401 ttttcggttc aactcaagcg ttgcaatgta agctgtaaac aaagctaatg attgccgatt
     2461 gtcttctgtt gattgattcg atagctttgt atgactttta gttgtccgtg tggcttaacc
     2521 ataattatga tttcacgatt cctcctgcca gtgccttgca caagctggag cgtaacccta
     2581 atagttattt tgttaatgta cttatatact tttatacttt tccattagaa acaatcacta
     2641 aatcgttgat accaaggtca gactctagaa tttcatcagt gaatccctgt attggttaac
     2701 cttcatcaat cccaaaaaaa tatatcaatg cttagcatca aaatgaatat atagtttaga
     2761 atacttggga ttcatcgata actgatcgct tatttggcca tattatttgg cacttggttc
     2821 aactaaacgc atttaggatg ttccttcatt ctcgtcatgg atgttctcaa accgtcatta
     2881 ctaataaact gtccaccgaa tcgatggttc atttcttgca ttttccacgt ccaattactc
     2941 tttttgctct ttatttgggc gtagtttcac tttcttaact tgtatcgtct tccatcaact
     3001 ccttaatcat cttcttagct ttattggctc ggtttccttg taaaaggtat ctacaaatag
     3061 tgacaatctg tattaaatct tcatcatttt ctgtaatgtc aataatttct atattctgta
     3121 ccatatattg caaagggttt cactaagttc ataaccgaag cgaattaaac aacttttgta
     3181 tagaataact accttttcta tttttccatt ggtaattcta tctattaatt ggtttaatcc
     3241 ttttgttgta gttgattcca ctaccaagat gattctctcg aattttaaag cttgaagatg
     3301 tattgctatt tttgctaact ggcgtatttg caactatttt tcccttctat tcttccttca
     3361 ttgattcccc tctaaaatta ctatccataa aaaattgcat aaatgacaat agtatttata
     3421 ttagtcatac tgctttttaa cacagcaaat tccagaaaaa cattcgttac atatttgtga
     3481 atttttccaa aaaataacac ttttataaaa cgacgaaaaa taaatcacta ccagtcaact
     3541 tctataatat taccaaataa caa
//
LOCUS       DARBEC010000149         3434 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.156, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000149 DARBEC010000000
VERSION     DARBEC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.156"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            19..2208
                     /locus_tag="SCL80_002707"
     CDS             19..2208
                     /locus_tag="SCL80_002707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000470930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEG1108537.1"
                     /translation="MFISTLQIRNFRNYESEIFRFDNETNTIIGENDSGKTNALTALR
                     ILLDDSYYYSSKTLKESDFFHGIQNGWQGHWIIISATFEGISEEEFDNEICASISLNS
                     ESQTILEELISNADKGVGSISLFIRPNKAIRKQLFDISTELDSHQFNEFRNSIRLSDY
                     EFYYTSKSNLNFCINENYDKLVGRLNDNIASNPEEDDEALLGSRIDMSDIFKHISVVY
                     VDALRDVLREMKNNRNPVKRIMETIESKISSDNVESLKTIIQQLNETITSVPEIRKIG
                     ENINRQLNSIIGSVYSPNLLLSSTMSDDMGSLAKFLSMKPEQNIDLDLLGLGHLNMIY
                     LALKIVEFEACRSRELLNIMLIEEPEAHIHHHIQKTLFEGLNLQKNYTQILMTTHSVH
                     LAEVSEISRMNVLKSFNGKSIVMNPANNLNSFAKEKLKKNNLNLINAVERYLDVKRNG
                     LLFSKGVILVEGDAEEILIPQIVKKLFGINLDELGIGLINVGSTSFEYIASLFDDDRI
                     QRKCAIVTDLDEQAIPSEHRLYKSTAQEKGQARKEKLSSLFNENNWVNCFYANTTFEI
                     EFLDVNKENILTYVSPIIDDAFKDKKTITAYKEEIQSEQYLERNLAMLKLANHVGKGW
                     FAIELGEKIDFLIKIPKYILNAIAFACQEIVDLSIYKKILLHTIDLYEETTELSALSA
                     SLKTSDTADLNIAIISLLEYFGKDEDVVYLINQIENYTKILYAEVSK"
     gene            2205..>3434
                     /locus_tag="SCL80_002708"
     CDS             2205..>3434
                     /locus_tag="SCL80_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000181735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEG1108538.1"
                     /translation="MTNIFEKIVNDLNLEQKDVVESEDNLYVNACPGSGKTRVLTRKI
                     AYQAIKHDGSLKRIIAITYTNRAAEEIKERLSLLSIDDDINIWVGTIHQFCLEFIIYP
                     FKMNLSRVSKGFTIIDDYTQRAYVSQINNMLNLNLKTYELNKIDLSLSHDFKINEVQY
                     TQVSEMYHTMLEKNKEIDFDMILLVAYKILTVNSNVARNIASNIRAIYVDEFQDTREL
                     QYNVIAKLLQNNPQIQSMFVGDIDQAIYGSLDGIAKSVEELEMLTSHTFQSKTLHGCY
                     RSNQRLVDFYSNFQSCPYEIESRGNNKNDRGFISYDCKVTKEDLPNIIKNIIKEKIES
                     GIHEKDICVLAPQHYPLFSLGEYLKKELPYCNFDAINISPIKVNNHSLFFKLAILYFT
                     ESGEKVRRRKLIANDILI"
BASE COUNT         1294 a          461 c          577 g         1102 t
ORIGIN      
        1 tagttaggag atctgcttat gtttatatct actcttcaaa ttcgaaattt tagaaattat
       61 gaatccgaaa tctttcgatt cgataatgag acaaatacta ttataggtga gaatgattca
      121 gggaaaacca atgctcttac tgcattgaga atcttattag atgatagtta ttactatagt
      181 agtaaaacac taaaagaatc agattttttt catggaattc aaaatgggtg gcaagggcat
      241 tggattatta tctctgcgac atttgaagga atcagcgaag aagaatttga caatgagatt
      301 tgtgcttcca tttctttaaa ttctgaaagt caaacaatct tagaagaatt gatttcaaat
      361 gcagataaag gtgtaggtag tatttctcta tttattagac ctaacaaagc tattagaaag
      421 caattatttg atatttccac tgaattagac tctcatcaat ttaacgaatt tagaaattct
      481 attcgtctat ccgactatga attttactac acatctaaat caaatctaaa tttctgtatc
      541 aatgaaaatt atgataaatt ggtaggaaga ctcaatgaca atatagctag taatcctgaa
      601 gaagacgatg aagctctgtt gggctcaaga attgacatgt cagatatttt caagcatatt
      661 tcggttgttt atgttgacgc tcttcgagat gttcttagag aaatgaaaaa caatcgaaac
      721 ccagtcaaaa gaatcatgga aacaattgaa tcaaaaatat catcagataa tgttgagtca
      781 ttaaaaacaa ttatacagca attaaatgag actattacca gtgttcctga aattaggaaa
      841 attggcgaaa atattaatcg gcaactaaat agcataattg gttcagtgta ttctcctaat
      901 cttcttcttt cctcaactat gtcagatgat atgggttctt tagcaaaatt tctttcaatg
      961 aagccagaac aaaatatcga cttagactta ctagggttag gacatctaaa tatgatttat
     1021 ttagctttga aaatagtcga atttgaagcg tgtcgttcaa gagaactact taatattatg
     1081 ttgattgaag aaccagaagc tcatattcat catcatattc aaaaaactct gttcgaagga
     1141 ttaaatcttc aaaaaaacta tactcagatt ctcatgacca cacattctgt acatttagct
     1201 gaagtgtctg aaatatctcg gatgaatgta ttaaaatcgt ttaatggcaa atctatcgtt
     1261 atgaatccag caaataatct gaactcattt gctaaagaaa agttaaaaaa gaacaatcta
     1321 aatctaataa acgcagtaga gagatattta gatgttaaaa gaaatggatt gcttttttct
     1381 aaaggtgtta tcttagttga aggagatgct gaagaaatac ttattcctca aatagtaaaa
     1441 aaattatttg gtataaactt agacgaactt ggaataggtt tgattaatgt tggtagtact
     1501 tcgtttgaat atatcgctag cttgtttgac gatgatagaa tacagagaaa gtgtgctatt
     1561 gtaactgatt tagatgaaca ggcaatccca tcagaacatc gattgtataa aagcactgca
     1621 caagagaaag gtcaagcaag aaaagaaaaa ttatcctccc tttttaatga aaacaattgg
     1681 gttaattgtt tttatgctaa tacaacattt gaaattgagt ttttagatgt gaataaagaa
     1741 aatattctaa cttatgtcag tccaattata gatgacgcat tcaaagataa aaaaacaata
     1801 actgcttata aagaagaaat acaaagtgaa caatatttag aaagaaattt agcgatgtta
     1861 aaactagcca atcatgttgg taaggggtgg tttgcaattg agttaggtga aaaaattgac
     1921 tttttaatca aaattcctaa gtatattctt aatgctattg cttttgcatg tcaggaaata
     1981 gtagatctct caatttataa aaaaattcta ctccatacaa tcgacttgta cgaggaaact
     2041 acagaattgt cagctttatc agctagttta aaaacaagtg atacagctga tttgaatata
     2101 gcaataattt ctttattaga gtactttggt aaagatgagg atgttgtgta tctaatcaat
     2161 cagatagaaa attacacaaa aatactttat gctgaggttt caaaatgaca aatatcttcg
     2221 aaaaaatcgt aaatgattta aatttagaac aaaaagatgt tgtggaaagt gaagataacc
     2281 tttatgtaaa tgcatgtcct gggagtggga aaacaagggt acttacaagg aaaattgctt
     2341 accaagcaat taaacacgat ggatcactaa aaagaataat tgctatcaca tacactaata
     2401 gagctgctga agaaattaag gaacgcctaa gtttacttag tatagatgat gatattaata
     2461 tttgggtggg gacaatacat cagttttgtc ttgaatttat tatttatcct tttaaaatga
     2521 atctctcaag agtatcaaaa ggatttacaa ttattgatga ttatactcaa agagcttacg
     2581 tcagccaaat taataatatg ctgaatttaa atctcaaaac gtatgaactg aacaaaattg
     2641 atttgtcact ttctcatgat tttaaaatta atgaagttca gtatactcaa gtttctgaaa
     2701 tgtatcacac tatgttggaa aaaaataaag aaattgattt tgatatgatt ttactagtag
     2761 cgtataaaat tttaactgta aacagtaacg ttgcacgtaa tattgctagt aatataaggg
     2821 cgatttatgt ggatgaattt caagatacta gagaattaca atacaatgta atagctaaat
     2881 tacttcaaaa caaccctcaa attcagtcaa tgtttgttgg agatattgac caagcaattt
     2941 atggatcatt agatggtata gctaaatcag ttgaagaact agaaatgcta actagtcaca
     3001 cttttcaatc aaagacgcta catggctgtt acaggtctaa tcagagatta gtagacttct
     3061 actctaattt tcagagttgt ccttacgaga ttgaaagcag agggaataat aaaaacgacc
     3121 gtggttttat aagttatgat tgcaaagtta ctaaggagga tttacccaac ataataaaaa
     3181 atataataaa ggaaaagatt gaaagcggca ttcatgaaaa agatatttgt gtacttgctc
     3241 ctcaacacta tcctctgttt tcactgggtg agtatttaaa gaaagaatta ccgtattgca
     3301 attttgatgc tataaatata tcaccaatta aggtaaataa tcatagttta tttttcaagt
     3361 tagcaatttt gtactttact gaatcaggtg aaaaggttcg aagaagaaag ctaattgcaa
     3421 acgatatttt aatt
//
LOCUS       DARBEC010000150         3129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.157, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000150 DARBEC010000000
VERSION     DARBEC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.157"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            103..1347
                     /locus_tag="SCL80_002709"
                     /pseudo
     CDS             103..1347
                     /locus_tag="SCL80_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            1360..1992
                     /locus_tag="SCL80_002710"
     CDS             1360..1992
                     /locus_tag="SCL80_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1108539.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKALRKEVKPLLDFLKKRDIP
                     LALASSSPAVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTAALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            2197..2424
                     /locus_tag="SCL80_002711"
     CDS             2197..2424
                     /locus_tag="SCL80_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEG1108540.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            2493..2759
                     /locus_tag="SCL80_002712"
     CDS             2493..2759
                     /locus_tag="SCL80_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1108541.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            2749..3018
                     /locus_tag="SCL80_002713"
     CDS             2749..3018
                     /locus_tag="SCL80_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1108542.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         1102 a          466 c          617 g          944 t
ORIGIN      
        1 tgaaattgct ttcctttaaa atgactcatc gtcattcctc ctgctatctt tttctattat
       61 tctaccttat ctgatagtag atttaaaact ttgcaacaga accagaaaac caaagatcag
      121 cttaacgaat tttttgctaa tgttcttgaa accccttatt ggtatgcaaa aaaaatctta
      181 agccttttat ctgagtgttt agatcaacct gtgtatgaag aggacatttt ctttattacg
      241 ctatattttt atagtttgaa tgtcacgaat gttaaaacag gtattaagag tattgtgcta
      301 gctcatggtt attctacagc tagtagtatg gcgaatgttg ccaaccgtat gctgaataag
      361 aacgtttttc aagcttatga tatgccttta gatattacat tagagaaaat tgaagcaaaa
      421 ttgattcaat tccttggaga ttatcgaaca gattcaggct tgattttatt ggtagatatg
      481 ggttcactaa accaactagg cggtttatta gtgaatcatc taaatggacc actgttaatc
      541 attgatcata tcaatacacc gttattattg gaagtcggcc aacaagtatt aaacaataaa
      601 agtatcaccg aaataggagc agaaattgaa acaagtctta ctttcaaaaa gcagttacta
      661 cttcccaaga aaaagaaaaa gaaagccatc attacttgtt gttatacagg gattggtagt
      721 gcgacccaga ttcaggagat tttaacgaaa tgcttgaaag atgcagttag agaattagta
      781 attattccgt atgattacaa aaagctgtta gaaaacaaga attttgagac tccttttcaa
      841 ttatacgatg tcatcatgat tgtaggtaca gaagatccca aaattaatca gattccatat
      901 attggattag atcgcctgat taatggggga agcattggcg aatttgttca ttacttggaa
      961 aaagagctga ctattgaagt gaacgattta caaaaagaat taattttcaa tttttcaatt
     1021 agtaaaattg tcgaaaattt aaccattttg gatccagata aggtgctatc ttttgtacaa
     1081 aaagcaatta aagaattatc gaagttatta aattttgatt tttctaataa tcagctgttt
     1141 ttgttgtaca ttcactgtag ttgtatgatt gaacgaattg tacgtaaaga aagtgtggat
     1201 gagcaagagg acattgaaga atatatgcaa gcaaatggag aaataatgga taaaattcat
     1261 agcgcttttt ttgagattga aagggaatac acaattgaaa taccagattt agagttgcga
     1321 ttattgagcg atattgttca aggataggag tgaattcgaa tgacaatggg agtaattttt
     1381 gatatggatg gggtactcat agatagcgag acattttatt ttgagagaag aatgaacttc
     1441 tttcaagaac tggagataga tccaggatca aaccagattg atgattatgt ggggaaaaca
     1501 gaagctgcaa tatgggaaac attagttcct gaggacaaat cattaagaga ggaactttat
     1561 ccgaaatatg tagattatcg aaaaacgcat ccaattgatt tcgctaaagc tttacgaaaa
     1621 gaagtgaaac cgttacttga cttcctcaag aagcgggata taccattggc actggcatct
     1681 tcttctccag ctgtcgagat agagagtatg ctcattcaat gtgaacttac tgaatatttt
     1741 tcctttgtta taagtggtga gtcattaaaa caaagcaaac cacatcccga aatatattta
     1801 aaagcgaaac agcaattaga ctgtgacttc tttattgcag tagaggattc aacgattggt
     1861 attaaatcag caaaggcagc aaatatctat acggcggcac taaaacaacc aatgcaaatc
     1921 gatcagagtg aagcagacat acaaattgaa agtctacaag aactggaaca aattgtttta
     1981 gaaaaaggtt agtgaattaa tggaaataag gctatatatc tgtgctagct attttgacta
     2041 accaaaaaaa cttgctataa ttcaggtgta gtaaaaggtg ctggtaacac cttttacgaa
     2101 aatgcttcgg cagtgataat gggattggct ttttaagttt tttattttgt tcgcaatccc
     2161 taaggtcatg tgaatgatcc tagggattgc tagaatattt tgcaacagaa ccaaaatacg
     2221 aatagttatc aagcagccat attgactgcg gtcaatttag gaggggatac tgatactact
     2281 gctgccgtaa ctggtgctct tgcgggtatt ctttacgggg gtgaagtgat ccctgaaaat
     2341 tggagaaaat cgcttcaaaa tgaccagctt cttaaaacga tcagtcagag attttgcgat
     2401 accgtgctat ctcaaagaat ttgaataatg gatatgatgc aaatcttgtt tgtactaacg
     2461 ttgtattgac aaaaaggatt ttatttagta caatgttagt acaaatgatg aacggaggta
     2521 gaatcatgtc tcaatcaacc attacattta ggattcccga cgatgaaaaa gaactggtat
     2581 tagaatatgc aaaagcacac aattcttcat tgaccgaact ttatcgaaat gctgtactgg
     2641 ataaaatcga agatgaaatc gatctacgga ctttacggag tgcaattgaa gtttccaaag
     2701 agaaaaaaga gagtggtatt tctcaagagg aaatggagaa actgctaaat gaattataaa
     2761 gtcatctaca tgccgcaagc ccagaagcaa ttaaaaaaaa tggacagaaa ccaagctaaa
     2821 ataattgtcg cctggatcag aaagaattta gaaaacacga ctgacccgcg tcagcacgga
     2881 aaaggtttga ctggaaataa gagtggggaa tggcgctatc gaattggaaa ctatcgaata
     2941 ttggctaata tttgtgattc tgaaattgaa atccagatat tctcaattgg acatagaagt
     3001 acaatatata aaagataacc gtttttggaa cgaagtagag tttcgaaagt caatgtataa
     3061 tcttttgaat tttgcataat accacaggta gaaactaccc gatactatcg gttggtttct
     3121 gctttttta
//
LOCUS       DARBEC010000151         3089 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.158, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000151 DARBEC010000000
VERSION     DARBEC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3089
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.158"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(34..389)
                     /locus_tag="SCL80_002714"
                     /pseudo
     CDS             complement(34..389)
                     /locus_tag="SCL80_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(649..1197)
                     /locus_tag="SCL80_002715"
     CDS             complement(649..1197)
                     /locus_tag="SCL80_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108543.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            complement(1198..2055)
                     /locus_tag="SCL80_002716"
     CDS             complement(1198..2055)
                     /locus_tag="SCL80_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1108544.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            complement(2341..2627)
                     /locus_tag="SCL80_002717"
                     /pseudo
     CDS             complement(2341..2627)
                     /locus_tag="SCL80_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2617..2946)
                     /locus_tag="SCL80_002718"
     CDS             complement(2617..2946)
                     /locus_tag="SCL80_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1108545.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
BASE COUNT          905 a          626 c          386 g         1172 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga accattaaat gttcttactg cctcttccac
       61 actaatttct tcgttcaatt tcactaataa tacttgatct cgcttattat aatcgctttc
      121 atttatccta agagtcaaat ctttttccac cataactttt acacccaata aattttgcgg
      181 tttatttttc tctgttgaaa ttctttgtgt agcctttaaa ctgacaatta gctcattggg
      241 tacatactta tttgagagtc atctaattgg ctcataatcc tttctttttg gttagtatct
      301 gcagttactt gtttagtatg agccatgaac ccaaacatac tcaccccaac aaccaatcct
      361 aaatatattt tctttttaaa ctttttcatc tacatctcct cctaatagtt ttattttaga
      421 gttagctcta ttacttattt actaactcaa caacagatta tcatacactt gttaatgaaa
      481 tcacaatatc tcattaaaac acaccctaaa atcataaccg ttcctatgct cattttttgg
      541 attttttaat cacgtaacca gaaaaataaa tcccctctat ttcacgcaaa tcaaagaaga
      601 actaagtaac taccgtttaa aggtagttac ttagttcttc tttgattatt atctaacttt
      661 attattcatt ccccaccctt tgcgcagtga gtcttcggta tcttgtttta tctgactggt
      721 agttcttatt atttctccac gatcatctaa ttctggaaca attggatatt tttctctgaa
      781 caagtctagt tctttcacca cgtcattaaa caaatcttgc ttatcttctt gcgtaagatt
      841 ctgtttaata tactcatcta aagcttttct aagcaattca tctcttgtca tattagtctc
      901 atacttttct actaaaactc tggaagtcgc atttaacaaa gtcgacagtt ttaaaggatg
      961 cttggtggaa taaaccttat caaactttct gattggtgcg ccaacttttt ttgttcttcc
     1021 tctactctgt ctttcaacat tggaggaagt ttttatttct tgaacttggt gcacttctaa
     1081 ttgcttattt tcagatggtg taattgcttg ctgctttttc tttgcttctg cttctttttt
     1141 tattttggcg agtcctttgt tgttcccaaa aatatcaagt cctttgatgt cagccaatta
     1201 ctccacctcc gcataccaat tctcgtgttc ttccaattct ttcaaaatac tgataaataa
     1261 ttcgtggctt ttgcgatccc attgttttaa gagacctttc tttgtcttct ccatcgtaat
     1321 acctgatccg tcgtatcgtt ttaagcgcgc atttttgagt acaacatttt taatcaaatg
     1381 ttccccataa atctcttggg cttgtttata catttcctga tctactgcgt caccgctttc
     1441 caacatgaaa ggaataacac cagcaacata taaatttgta tcaaattctt tcacaaaaaa
     1501 gtccatatac tcaagatatt gcatcgcatt tctcatcgat tttacttggg tctgtaagac
     1561 gacgattgcc caatccgcaa tgtacatcgc ggatttacta tatacagaaa tagaaggtgg
     1621 gacatctaaa tagatgacgt caaactcatc aaataatggt tctagctttt ctttcatata
     1681 tttgaattgg gataatgaat cattatattg gtctttgatc caatcatcat acatttcaaa
     1741 ggacatgtct gctggaagta tatagaggtt attgattact ttatagacag cctctttagg
     1801 tggaagattg tttatcaaca cgtcagtcac agtattttcg taaacaatcg gttcaccttc
     1861 tccgcctcct tcttcgttag tgaagttcat atgcgttaag ttcatcacat cagaagtatt
     1921 tccttgcata tccaagtcaa ttatcaaggt tctttttcct tcattaactg ctgattcaaa
     1981 ccctaacatt tgcgcgcacg tacttttacc tacgccacct ttaaaattac cgattacata
     2041 tttcttcgct accacgttcc tacctctttt cttttagcaa tattcatttg atactattat
     2101 aacatattta tttttagtta ctaccaaaaa aaagatatat atgtactacc ttataattaa
     2161 taagtaaaag gtagtacata aataaggaaa agctgaaata tattgattta tagctttcct
     2221 caaagtaatg acgttattta atttatcgag aagcagagaa tactcgcgac tttagtcatg
     2281 tagacgaatc agattttcga taaaaaagaa gccgtccttc ttaagaacga caacaaatca
     2341 ctagattaat tttttcaact gttcataaaa gttttcatgc gttccacata acaatactgg
     2401 aataaccttg ttttctataa tctcgtaagc tactctatat gttgtgcccg catatttgaa
     2461 cccactagac caaatacctg ataaatcccc agttttttgt tcacccttat atggatcaat
     2521 cgcaatttca tcataaatta aattcaaaaa tttttctttc aatggcttct cttttagctt
     2581 tttaaatact taatgacatg ttttgaagga acgatttcat aagccaattt ctgttgccaa
     2641 ctcctcttta gtcattactt tagaattagt aagtgtatct tcagcaatat ctctaaagga
     2701 agaatgcatt ttgttttttc tattcttaaa ttctacaaga atctcatctt tatcgtagcc
     2761 ttctgcaatt atatcagaca aaatatcttc gctaaaatca taaaattctt tttgtggttc
     2821 aacaaattca taaagaatac cattctctac caactttgct tcaacttcga ctccattagg
     2881 aacattaaaa tctttaggta ctgtcaacat gattgaattg ccttgttttc ttgtttttgt
     2941 aatcatttaa atccctcatt tctaacagta tgtaattact tagtaaatac attataccac
     3001 gagataatgt atttacaagt aattactgtg actcgtaatg cgtccttgct gaataaattt
     3061 caaccacgtg ttcatcttca tctacctta
//
LOCUS       DARBEC010000152         3026 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.159, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000152 DARBEC010000000
VERSION     DARBEC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.159"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(292..654)
                     /locus_tag="SCL80_002719"
     CDS             complement(292..654)
                     /locus_tag="SCL80_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108546.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
     gene            complement(728..1108)
                     /locus_tag="SCL80_002720"
     CDS             complement(728..1108)
                     /locus_tag="SCL80_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEG1108547.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            1172..2365
                     /locus_tag="SCL80_002721"
     CDS             1172..2365
                     /locus_tag="SCL80_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1108548.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            complement(2442..3026)
                     /locus_tag="SCL80_002722"
     CDS             complement(2442..3026)
                     /locus_tag="SCL80_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEG1108549.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
BASE COUNT          944 a          579 c          498 g         1005 t
ORIGIN      
        1 aactattttt taaatttttt agaaatgtga tataacatcc acttcagaat caacgctgtt
       61 aaatttatca tgtatttctt ccttggtcaa ctataaatta agcattactt tgtgaaatga
      121 tttttcgatc aattattatt attcgttata caaccgtttt caacatattt tgaaaataaa
      181 atgcactaaa aaactatctt tagcagaaca tttcattgta ttttttggac caaacaataa
      241 aaaaagaagt ttgaaaaaaa ttcaaacttc tttgttcgct taatctgtat tttagaagaa
      301 aggcactccc tctacttcgt aagttccaag aacagagcgc atttgacctg gttcttccca
      361 aatatatcct tcgcctaatt cttctctttg attcttttgt tcagtttcaa agacaaacaa
      421 cggaacattt tcctctttaa tatgtgtatt tgtcaattcc atcaaactaa tcttgctaac
      481 atcgatatga actatttctt ttaagaaatt aagtatattc gctaaaccag tctttccttc
      541 agaaagttcc gctaacgcaa attccaactt gtcatcaatt gaatggacca aaaatttttt
      601 agtaccatcc tccaaatgca acataattgt accagcaact cttttgcttg ccaaaatttc
      661 cacctcgatc aacttgttct atggcttaca taaaaattct agcacatttt cagaaaaaat
      721 acacttttta tttcttattt tctttccgtt ctagttcaga taatgcttct tctatccaaa
      781 taggtaactg ctccgcaata gtggtaaatc cgatcttacg atttttattc gtgaaatacc
      841 tttttctgcg atatggaaca gctggatttg ttttttctaa cgttcttaaa gataaactga
      901 tttttttgga atattcatca atgtcgatga tctgaactgt tactggctgt ccgactttta
      961 acaatgaatg gatatttttc gtataaccgg attgtacttc tgaaacatga ataagtcctt
     1021 gcgtctcgtt atcaagagaa acgaatgcgc cataaggctg tatccctgtc acttttcctt
     1081 caattatttg tccaatttta taagtcatct acttccctct cttcgcattt atcattatac
     1141 tgtaaatagc tcaaaaaaag aagcgacgaa aatgactgga ggtcttaaga tgatcgaatt
     1201 tgataaacag atttcaagaa aagatacaga aagtgtaaaa tgggacgcaa ttgctgagac
     1261 atatcaaatg gatgacttac ttcctttgtg ggtagcagat atggacttct tgtctcccgg
     1321 tggggtagct aaagcgttta gtgaatacat caaacatgga atctttggtt atgccactct
     1381 gtctgataaa ctatatcagg ctatcattca ttgggaacgc caacaccatg ctgtggagtt
     1441 gacgaaagaa aatatcgttt ttacaagtgg tgtgttaacc agtttagctg cagcagtaca
     1501 aacttttaca aaaccaggcg attctatttt gattcatgac ccagtttatc ctccattttc
     1561 ctctattata gaaacaaatc aacgtcacat cgttcgaagc tctttgctag aaaaaaataa
     1621 tcatttcatc atggacttgt ctgacatgga gaaaaaaatc gaagaaaaca atgtaaaagc
     1681 aatgatttta tgcaatcctc acaatcctgg agggcgtgtt tggagcaaag aagaattgaa
     1741 acagttaagc gaattgtgtt taaaacatca agtgattctg tttagtgacg agatccatca
     1801 agatttaact ttattcgatc atacatttac ttccatgcta acgattgatc ctaatttaga
     1861 tgatctacta gttgctttta cttccgcaac caagactttc aatctagctg caatcaaaaa
     1921 ttcaatggta tttatcaaaa atccagagat gaaagctgat tttgaaaaac atttagtgat
     1981 gaaccaacag cacgaaatca acacttttgg actgattgga acccaagcgg cttatgagac
     2041 gggtggagaa tggttaagcc aactgattcc ttatctagaa aaaaatgttc aaacagttaa
     2101 tcagtttttt gaacagtatc ttccgaaagt gagaataatg aaccctgaag gaacttactt
     2161 aatgtggtta gattttaccg cctatatttc agacgaccaa gaattggaga acactcttgt
     2221 tcggaaagga aaagtcgtat taaatcctgg catcacgttt ggtccttccg gtcatgggca
     2281 tatgcgcttg aatattgctt gtccagaaga aactttactg gaaggactta atcgaatcaa
     2341 gtatgcttta gagacaatcg attaaatcat ttattcaaaa taaaaggacg agaccttact
     2401 aggtttcgtc ctttttcata attactccgt accataccaa actattctga aatttcaatt
     2461 gtttcaatca ctacatcatg tacaggtcgg tcttgtggac cacgttgtac atttgcgatt
     2521 tcatctactg tgtccatccc ttctagtaca tgtccaaaga ctgtatgtcg gaaatctagc
     2581 caaggagttc cgccaccttt atacgcttca atgatttctg atgggaatcc agcaccttct
     2641 agttgtccaa gcatattagc tggcacattt gtgttattta caataaagaa ttggcttccg
     2701 tttgtgtttg gacctgaatt tgccatagat aaagctccgc gaaggttgaa cagttcacga
     2761 ctgaattcat cttcaaaact ttcgccataa atactttcgc cacccattcc tgttccagtt
     2821 ggatcgccgc cttggatcat gaaatcagga ataacgcggt gaaaaatcac gccatcataa
     2881 taaccttttt tggaaagttc aatgaaattt tgaaccgtct tcggtgctag ttcagggaat
     2941 aattgaacag taatgtcacc acggtttgtt ttgataacag cttttggtcc tttttcactt
     3001 tttaaatcta gttgtgggaa tgccat
//
LOCUS       DARBEC010000153         3012 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.160, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000153 DARBEC010000000
VERSION     DARBEC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.160"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            177..359
                     /locus_tag="SCL80_002723"
     CDS             177..359
                     /locus_tag="SCL80_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEG1108550.1"
                     /translation="MINLFLLVTASTLFFTGISLIISYLIYKKAENHTSKAIGLAVSS
                     FLTLLTLFVTLSFSFV"
     gene            462..746
                     /locus_tag="SCL80_002724"
     CDS             462..746
                     /locus_tag="SCL80_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108551.1"
                     /translation="MLTATLQQPTHVAIAESYTINAGIVKQLIKDREFKKFNSRQAYN
                     RQLKKAILKHKDSFIGNLESVDVKTVSTAFSVVEVTIDGRLISLFAFGIN"
     gene            762..998
                     /locus_tag="SCL80_002725"
     CDS             762..998
                     /locus_tag="SCL80_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108552.1"
                     /translation="MEGKTFAEIWTEFIEVPTRIDPITSEKVLDADFYNLKAGTSLEC
                     VWMYLEEHFKGVTLGHVLYHNGAEWDAIGNIHFS"
     gene            1020..1274
                     /locus_tag="SCL80_002726"
     CDS             1020..1274
                     /locus_tag="SCL80_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108553.1"
                     /translation="MNYTDEEQIALEYVFTNEEGEEIEKVLIISSKDLEKALAYEPYE
                     STVTVIEFLDTYTSNDTWEIMNICNANNIQFAEKTNKSFY"
     gene            1356..1541
                     /locus_tag="SCL80_002727"
     CDS             1356..1541
                     /locus_tag="SCL80_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HEG1108554.1"
                     /translation="MPKTAREIVKILKSNGFKKTGQTGSHAKFYNSKTKRTVVVPIHN
                     GDVPLGTEANIWKQAGL"
     gene            1608..2003
                     /locus_tag="SCL80_002728"
     CDS             1608..2003
                     /locus_tag="SCL80_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1108555.1"
                     /translation="MKTKNIFYPAIFEKEGEAYNIVFPDLPEISTFGNSLEEAYLNAK
                     DALGLALYSVPDAEFPKPSAVEELTLKANQVVVIIQLNIKLFRRSLNTKTIRKNVSVP
                     EWLVLLGKEKNINFSQLLQKALEEELLEK"
     gene            1993..2370
                     /locus_tag="SCL80_002729"
     CDS             1993..2370
                     /locus_tag="SCL80_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108556.1"
                     /translation="MKSKKETEPMKATHLNYFSRTLINNLLKDTTGISVSTKHFANDL
                     IIFEDNNLRIEMFIEQATPNSIPEFCYTHTEKMKHAKSVKDVEALSESLTNALNLVKK
                     AKLLFEFQTDYHNELLAQSEILD"
     gene            2392..2766
                     /locus_tag="SCL80_002730"
     CDS             2392..2766
                     /locus_tag="SCL80_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108557.1"
                     /translation="MKQTQTETVNDTLADQLAGYYKLFEMGKAQRLIHYLWKYKILFL
                     FWESTLTFSVLFYKSDFYNQHLVLGSFLLIYTILMLNLYTYITLEEHLVISDAGIERN
                     IKRVSFKITLNKPTDKKLSEAF"
BASE COUNT         1058 a          507 c          523 g          924 t
ORIGIN      
        1 tttatgttta tgtatatgta tatgaatata aacgtattag gtacgaacac ttttaaattc
       61 ttttctgggc tttgttttta gggtagggct aaaaaaaaca tcaccatctg atccaaaaca
      121 gctcagaaac gcattttaac gtatctgata ccaatattac agaaggagtg tatcgtatga
      181 ttaacctatt tcttttagtg acagctagta cattattctt tactggaata tcactgataa
      241 tttcctattt gatctataag aaagccgaaa atcacacaag caaagcaatc ggactagccg
      301 tttcatcctt tttaactttg ctgactttat ttgttacttt atcgttttct tttgtttaga
      361 acagctgatc cattagcacc tttttctctt tacattatac acactgttgt gtataatgta
      421 tttgtaaaca agctaattaa cttttaggaa ggagtgacaa gatgctaaca gctactttac
      481 agcaacctac tcacgttgct attgcagaaa gttatacaat taatgcaggg atcgttaaac
      541 agctgatcaa ggatcgagaa ttcaaaaagt ttaacagtcg gcaagcgtat aacagacagc
      601 taaagaaagc tattctaaag cacaaagatt ccttcatcgg gaacttggaa agtgtagatg
      661 ttaaaactgt ttcaacggca ttttcagttg ttgaagtgac tattgacggt cgcttgattt
      721 ctctatttgc ttttggaatt aattgatagg gagtgtgaac tttggaaggt aagacttttg
      781 cagaaatttg gactgaattt attgaggtac caactcgcat tgatccgata acttctgaaa
      841 aggtattgga tgcagacttc tacaatctca aagcaggtac tagccttgaa tgcgtttgga
      901 tgtatttaga agagcatttc aaaggcgtaa cgctcggtca cgtcttatac cacaatggtg
      961 ccgagtggga tgcaattgga aatatccatt tttcttaata taagaaagga gaacacaata
     1021 tgaattatac cgatgaagaa caaatcgctt tggaatacgt tttcacaaat gaagaagggg
     1081 aggaaattga aaaagtcttg atcatttcat caaaagactt ggagaaggct ttggcttatg
     1141 aaccatacga atcaaccgta actgtcattg aattcttaga tacctatact tctaatgata
     1201 cttgggaaat tatgaatatt tgcaacgcta ataacattca atttgctgaa aaaaccaata
     1261 aatcatttta ttagttttgc aataaaacat cctctttttc tattgattcc atacacaaca
     1321 atgtgtataa tagattttgt aaagggaggg tagctatgcc taagacagca agagaaattg
     1381 taaaaattct caaatctaac ggcttcaaaa agactggaca aactggcagt catgcaaaat
     1441 tttataattc caagactaaa agaacggtcg ttgttccgat ccacaatggt gatgtaccac
     1501 taggaacaga agctaatatc tggaagcaag cagggcttta acagccttgc cttcctattt
     1561 ggtattaggc atactatcct ctcttacact agaaaaggag cgaggaaatg aagacaaaaa
     1621 atatttttta tccggcaatc ttcgagaaag aaggagaagc ttataacatc gttttccctg
     1681 acttaccgga aatatctacc tttggaaata gtcttgaaga agcatattta aacgctaagg
     1741 atgctttagg attagcgcta tatagtgtac cagatgcaga atttcctaaa ccatcagcag
     1801 tggaagaatt aactttgaaa gccaaccaag tggtagtaat catccaacta aacattaaac
     1861 ttttccgaag aagcttaaat acaaaaacaa ttagaaaaaa tgttagtgtt ccggaatggt
     1921 tagttctttt agggaaggag aaaaacataa acttctctca actacttcaa aaagcactag
     1981 aagaagagtt gcttgaaaag taaaaaggag accgaaccta tgaaagctac acacttaaac
     2041 tatttctctc gaacgttaat caataatcta ttaaaagata caactggcat tagcgttagt
     2101 accaaacact ttgccaatga tctaattatt tttgaggata ataacttgcg aattgagatg
     2161 tttattgaac aggcaacccc taactcaata cctgaattct gctatacgca cactgaaaaa
     2221 atgaaacatg caaaatcagt taaagatgtt gaagcattaa gtgaatcact aacgaacgct
     2281 ttaaacctcg ttaagaaggc aaaattgtta tttgaatttc aaaccgacta ccacaatgag
     2341 ttgcttgctc aatctgaaat tttagattaa ttgaaacagg agaagagaaa gatgaaacaa
     2401 acacaaacag aaaccgttaa cgatacgctt gctgatcaat tagcaggtta ttataaatta
     2461 tttgagatgg gtaaagcaca gcgcttgatc cactatcttt ggaaatacaa aattttgttt
     2521 cttttttggg aatcaacact aacattctca gttctttttt ataagtcaga tttttataat
     2581 caacacttag ttttagggag ttttttgctt atctacacca ttttgatgct taatttatat
     2641 acctacatca cacttgaaga acacttagta attagcgatg cagggatcga gagaaacatc
     2701 aaaagggtat ccttcaagat cacgttaaac aagcctaccg ataaaaagct ctcagaagcg
     2761 ttctgagagc ttttgacatt ttcttgtata attttatcct aacacattac aaagcaccta
     2821 attcgagctt atatcaagca aatttgctat ttattactca atacatatct aaaaattact
     2881 tgatggaaaa ttagatacat ggtaagcatg aaaagaaaat caatttgagc agatgcagta
     2941 caatacatag caatttaaaa gctctcagaa ggcttctgag agcttttaat atattctttg
     3001 ataatttgtc tt
//
LOCUS       DARBEC010000154         2948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.161, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000154 DARBEC010000000
VERSION     DARBEC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.161"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1350
                     /locus_tag="SCL80_002731"
     CDS             <1..1350
                     /locus_tag="SCL80_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEG1108558.1"
                     /translation="TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDT
                     VKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNVTNLPALPEGTTVTDVTPGGT
                     IDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNADKYNPEGQKVTTDLNKEPDA
                     SEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTYPDGSSDEVEVDVTVTDTRSD
                     ADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYTWKEKVDVSTAGNKKGTVVVT
                     YPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTGKTEPNAEVTVTLPDGSQHHG
                     TADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNVVVSSNKKDSGKNGSKGSKTD
                     NQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGLILLGTLGLLGYKNRKKENE"
     gene            1484..2827
                     /locus_tag="SCL80_002732"
     CDS             1484..2827
                     /locus_tag="SCL80_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEG1108559.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNFILKAVTGLNIET"
BASE COUNT         1171 a          398 c          561 g          818 t
ORIGIN      
        1 actaacttgc cagcgttacc agaaggaaca acagtaacag atgttactcc cggcggtaca
       61 attgatacca acacaccagg taattacgaa ggtgtcattg aagtaacgta tccagacggt
      121 acgaaagata cggtgaaagt tccagtagaa gtgacagaca atcgttctga cgccgataaa
      181 tatgaaccaa cagtagaagg cgaaaaagta gaaattggag gcacagtaga tttaacagat
      241 aatgttacta acttgccagc gttaccagaa ggaacaacag taacagatgt tactcccggc
      301 ggtacaattg ataccaacac accaggcaat tatgaaggtg tcattgaagt aacgtatcca
      361 gacggtacga aagatacggt gaaagttcca gtagaagtga cagacaatcg ttctaatgca
      421 gataaataca atcctgaagg acaaaaagtg actacagact taaataaaga gccagatgca
      481 tctgagggaa ttaagaacaa agaagattta ccaaaaggaa ctacttatgc ttggaaagag
      541 aaagtagatg ttagcacagc tggaaataaa aaaggtacta ttgtagtgac atatccagat
      601 ggatcatctg atgaagttga agtagacgtt acagtgacag atactcgttc cgatgcagat
      661 aaatataatc ctgaaggaca aaaagtgact acagacttaa ataaagagcc agatgcatct
      721 gagggaatta agaacaaaga agatctacca aaaggaacta cttatacttg gaaagaaaaa
      781 gtagatgtta gtacagctgg aaacaaaaaa ggtactgtag tagtaacata tccagatgga
      841 tctaaagaag aagtagaagt tactatttct gtagtagata aaaaagcacc gaataaacct
      901 caagttgatc ctattacaga gggtgaccaa attgttactg gtaaaactga accaaatgca
      961 gaggtgacag ttacattacc agacggaagt caacaccatg ggacagctga taaaaatggt
     1021 aattttacag ttaaagttcc taaattagag gcagggacaa aagtgatagt aactgcaact
     1081 gatgaatctg gtaatactag tgaacctact aatgtagttg tttcttccaa caaaaaagac
     1141 agtggaaaaa acggtagtaa aggtagtaag actgacaatc aaggtagcaa cagcaatcaa
     1201 gacaaaaata gaggcaaaag ccagtcctca aaagtttttc ctaagacggg agagagtgac
     1261 agtaatatct ttactattag cggtggttta atactgttag gaacgttagg attattaggg
     1321 tataaaaatc gaaaaaaaga gaatgaatag aaaatcaatt tcttaagtct tctctgagaa
     1381 gaggaagaat ctttttcttc ctctttttgc ttttttatga tgggaaataa gtgttagtta
     1441 aagatgaata tactttttat agaaaggaaa agaatgtaat taagtggaaa aaatagtaat
     1501 tattggttca aaccattcag ggctagccgc agctcgattt ctaaacttaa gcgattcaaa
     1561 ttatgaagtt attctaatcg ataaaaaatc agaaattagt tacttggaat gtgggacatc
     1621 gttatttata aaaggagaag taagtaattt agaagatctt ttttatcaaa atagaatgac
     1681 actaaattct gagataaaaa agttatttct agagacagaa gttgacaaaa ttgatattca
     1741 aaataaaaag attagttgtg taaaaaaaga tggtacaaga ttctctcaag aatatgacaa
     1801 agttatatta gctacaggtt caaagcaaat tatgctagat ataccgggga ataatttagc
     1861 taatattttt tcgataaaga attttccaaa tgcaaaagag atttttaaag ctttggatga
     1921 tagtactatt aaaaaagtag gaattattgg agcaggatat attggagttg aaattgctga
     1981 agccataaga aaaagaggga aagaagtata tcttttcgat gttgcagata gagtgttgtc
     2041 gacttattac gaccgtagtt tttcagataa agttgaagaa atactatcaa aaaatggaat
     2101 acatctttgt ctatcagaaa gtcctaaaaa atatttagga agaaaaaaga ttgaaaaaat
     2161 agtcactaca agtggtaccc aatactctat ggatatggtc gtacaagcta taggatttct
     2221 atctaatagt ccaattgggg aaaaggaact cttgagagat gttagtggag catatctaac
     2281 taatgaatac cagcaaacaa atataaaaga tatatatgct attggggatt gtgctacaac
     2341 tttttttacg tccattaata gaagtagtgt tgatttttct atttctaatg ctctaagaac
     2401 agcttatatt gcaactcaac atattaacaa tcaagatttt acaccttctg gttcccaatt
     2461 tacaaatggc ttttctatat ttaattacaa tttttacgca gcaggtttga atttaaaaac
     2521 agcacgatta ttaagtgagt ctatagatta tatagaaata gaagatttca ttagacctgc
     2581 ttttttgaga aaaaatagtc gtgtaaaatt aagaattagc tttgaaaaaa atacaaagag
     2641 aatagtaggt gtacaacttt gtagtcaaga agatttatct ggactattat caatgttttc
     2701 tcttgctata gaagaacaaa ttacattaga caaattgaaa atgttagatt atttatttca
     2761 tcctagtttt agccaaccct ataattttat tttaaaggca gtaacaggat taaacattga
     2821 aacataatcg ttactaaatg aaatcaagaa atatactgta ttactcaatt cattgaaaat
     2881 atttatcata atttttaata gaaaacacta ttaaagagaa aaaagagagg taaatcaaat
     2941 gaaaaaat
//
LOCUS       DARBEC010000155         2827 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.162, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000155 DARBEC010000000
VERSION     DARBEC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.162"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            799..1053
                     /locus_tag="SCL80_002733"
                     /pseudo
     CDS             799..1053
                     /locus_tag="SCL80_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002405927.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
     gene            1123..1635
                     /locus_tag="SCL80_002734"
     CDS             1123..1635
                     /locus_tag="SCL80_002734"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108560.1"
                     /translation="MTNKNNERYSTELKATAIKKMMPPENRGIRQLSQTLGIPEATLY
                     SWRKKARIQGNTTPGTKKNVEKWSSEDKFLIVMESFSMNQVELAEYCRKKGLFKEQIE
                     AWKKTCLSANEQEENRTRELATELKEEKKQARQLEKELRHKEKALAEAAALLLLRKKA
                     HAIWGDQEEE"
     gene            1632..2669
                     /locus_tag="SCL80_002735"
     CDS             1632..2669
                     /locus_tag="SCL80_002735"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1108561.1"
                     /translation="MISSSDRKQAVELIQEANRNGARLTYACNELNISVRTYERWTRE
                     GTIAHDQRPLAKRPVPKNKLTDQEREKIIETVSKKEFMNLPPSQIVPKLADCSIYIAS
                     ESSFYRVLREKNMQHHRGRSQVSQKRIPPSHLATKPNEVWTWDITWLKGPIKGLFYRL
                     YLIIDLFSRKMVGWEIWESEEARHAEKLIKQTVLKERIQGQPLVLHSDNGSPMKAATF
                     QVLLEKLGIQSSYSRPRVSNDNPYSEAAFRTLKYRPEYPNNGFKTIDDARKWTKEFVD
                     WYNDSHLHSGIQFVSPAACHEGRHLAILEKRKQVYEDAKKRHPERWSKGVRNWTPHQS
                     VALNPLKETES"
BASE COUNT         1023 a          496 c          564 g          744 t
ORIGIN      
        1 attgatgacc acgaaagtgc atggcgatat ttgatgttga tactttatgc agtaccctat
       61 gtattcttcg ctatcattct gtggctcata gcattgtttt ttagagtatt taatgacctt
      121 taggtagatt tatatataaa aagggctaaa tacgcaaaat acccccttaa aatgcaaaat
      181 aaatgcaaat aagggggtaa ctccttatga aaaaacaact aatagtatta taacacgttt
      241 ttatgccgag aaaacttatt ggttggaatg ggctatgtgt tagctaactt gttagcgagt
      301 tggttggact tgaattggga ttaatcccaa gaaagtacca actcaacaac acataaagcc
      361 ctgtaggttc cgaccaataa ggaaattgga ataaagcaac gaaaggagtt gagtaaaatg
      421 agtgagaaaa cgaagaaaat cattcgagta tcattagctt tttcacaagt agtagtgcaa
      481 tcattgatag tttattatac ttaccagcaa tataaattat ctaaagagaa ttaagttttg
      541 attatgtaat aagttttggt actaaaaaag acttgatcta attagaccaa gtcttttgtt
      601 agtgttatat taataacaaa ataaaaataa gaagtcgctc actccccgac caaagtttgt
      661 gagcaaaata aacagtcgat gtttgtttct taattgtata tcttgatatt aaacgattta
      721 agattattct tcaagatata tattcgcaaa tgtaattgga aaagcgactt cttaaacaaa
      781 attaaggagt cgctttttat ggcaaaaata acgaaaaatg agcaagaaaa tttggtggaa
      841 aattacacaa aaaaaccggc gtactctaat agccggttag acgcacattt cgtgtgcact
      901 tctaatccga aattgagctt tgacgctatg acgatagtcg gaaaccttaa taaaaacagc
      961 gctaaaaagc tatccgattt tatgagttta gatccacaaa ttagactttg ggatatactt
     1021 caaacaaaat ttaaagctaa agcactacaa gaccccctat gttaggctag ttgtcgtata
     1081 ggataataag ttataataaa gactatacgg aggatgatta ccatgacaaa taaaaacaat
     1141 gaacgttact ctaccgaact gaaagcgaca gccatcaaga aaatgatgcc acccgaaaat
     1201 agggggattc gccaattaag tcaaactttg ggcattccag aagcgactct ttattcttgg
     1261 cgtaaaaaag ctagaattca agggaatact acacccggaa cgaaaaaaaa tgtcgaaaaa
     1321 tggtcaagtg aagataaatt tttaattgtg atggaatcat tctcaatgaa ccaagtagaa
     1381 cttgcggaat attgccgaaa gaagggctta tttaaagaac aaattgaggc ttggaagaaa
     1441 acgtgcttat ctgcgaatga acaggaagaa aatcgaacga gagaacttgc aactgaacta
     1501 aaagaagaga aaaaacaagc gcgtcaatta gagaaagaat tacgtcataa ggaaaaagca
     1561 ttagcggaag ccgctgctct cttattacta agaaaaaaag cacacgcgat ttggggggac
     1621 caagaggaag aatgatttca tcttcagatc gcaaacaagc tgttgaactt attcaagaag
     1681 ccaatcgcaa tggggcccgt ctcacctatg cctgtaatga gctgaatatt agcgttcgaa
     1741 cctacgaacg atggacgcgt gaaggtacga tagcgcatga tcaacgccct ttggcgaaac
     1801 gtcctgtacc caaaaataaa ctgacggatc aagaacgcga aaaaataata gaaaccgttt
     1861 caaaaaaaga atttatgaat ttgccaccta gccaaattgt accaaaactt gcggattgct
     1921 cgatttatat tgcatcagag tctagcttct atcgtgtatt gcgagaaaaa aatatgcaac
     1981 atcaccgtgg ccgaagtcaa gtttcacaaa aacgcatacc gccaagtcac cttgcgacga
     2041 agccaaatga agtttggact tgggatatta cttggctaaa aggtccaata aaaggactct
     2101 tttaccggtt atatctcatc attgatttat ttagccgtaa aatggtaggc tgggagattt
     2161 gggaatcaga agaagctcgt catgccgaaa aacttattaa acagacggtg cttaaggaac
     2221 gtatccaagg acagccgttg gttttacact ccgataatgg cagccctatg aaagccgcga
     2281 cttttcaagt cttactcgaa aaattaggaa ttcaaagttc ttattcaaga ccacgagtga
     2341 gtaatgacaa tccttattcg gaagcggctt tccgaacgtt aaaatatcga ccagaatatc
     2401 ccaataacgg ctttaaaaca atagatgatg ctcgaaaatg gacgaaagag tttgtcgatt
     2461 ggtataacga ttctcatttg catagtggga ttcaatttgt gtctcctgca gcgtgtcatg
     2521 aaggacgaca tctcgctatt ttagagaaac gaaagcaggt ttacgaggac gctaaaaagc
     2581 gccatcctga acgctggtca aaaggtgttc gaaattggac tccacatcaa tcggtggcgt
     2641 tgaatccttt aaaagaaaca gaaagttaac aggagcataa tctttcaaga ttatcaaata
     2701 accctcgtca cccaataggg gaaaaagtta ttcttcccct attggataac gaggccaacc
     2761 gaccataggg aggttctgct ttttgatagg tggaaaataa aaatacgaca actatattga
     2821 caaacac
//
LOCUS       DARBEC010000156         2739 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.163, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000156 DARBEC010000000
VERSION     DARBEC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2739
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.163"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            700..2610
                     /gene="ltrA"
                     /locus_tag="SCL80_002736"
     CDS             700..2610
                     /gene="ltrA"
                     /locus_tag="SCL80_002736"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEG1108562.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQKDKLVSQAKKI
                     ASPSKGKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFTNRLGTNKRG
                     RLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVYLANCYTLEG
                     RKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITLEEFQQANEI
                     HCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNKVQLIRLNKY
                     RVLAGNQELNLI"
BASE COUNT         1072 a          425 c          558 g          684 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaagacaa gctggtatca
     1861 caggcaaaga aaattgcttc tccctcaaaa gggaaaacgg aattagaaga aacaagactt
     1921 tataattcaa tggtattagg tatgcaaaat tattatcaga ttgcaacgaa tgtaaatctg
     1981 gattgtatga aactgaatag agctgtaatg gctatcttca ctaacagatt aggtacaaac
     2041 aaaaggggca gattgagaaa aacaggcaga caacttacga caaaggaggc aacaagatat
     2101 ggtaaatcta aaatgcttcg gtatgttgct ggcgagggcg aacctatttt ccctgttgga
     2161 tatatccaac acagaaaacc aatggcaaag gtgtatttag cgaattgcta cactctagag
     2221 ggaagaaaat ttatccacac caatctaagt gttgataaat tactgatgta ccagttaatg
     2281 aaattatctt tggaaaatcg cacaatagaa tacgcagaca atcgaatttc gctattttct
     2341 gctcaacatg gaaaatgtgc tataactctg gaagaatttc aacaagctaa tgaaatacat
     2401 tgtcatcata ttgtaccaac tggagctggt ggaaatgacg attataaaaa tcttattttg
     2461 gtaaaagagg cggtacaccg tctaatacac gctaaaacag aagaaacaat acaaaaatat
     2521 atagctctat tgaagctaaa taaagtccag ctcataagac taaataaata tagagtatta
     2581 gctggcaatc aagaattaaa cttgatatag gtctactaca taaatacttt tcaatataaa
     2641 cacgatggaa cgcggagtgc ggtgaaagtc gcacgctccg tgtgaatggg gggaaaagtt
     2701 tgagatattt ataatatcag gaacttacct atccatata
//
LOCUS       DARBEC010000157         2727 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.164, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000157 DARBEC010000000
VERSION     DARBEC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.164"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            27..377
                     /locus_tag="SCL80_002737"
     CDS             27..377
                     /locus_tag="SCL80_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108563.1"
                     /translation="MSRLIIQTAFYKAIIPENLDEVKLYLWEDNGEIVGFSGVSAEEL
                     VMLFLDPDFIGKRYGSQILIELIETEDIKRIDVNSQNKYAKHFYLSHGFEIESEEDLD
                     GFGKPYPITHLVKK"
     gene            791..1609
                     /locus_tag="SCL80_002738"
     CDS             791..1609
                     /locus_tag="SCL80_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1108564.1"
                     /translation="MNKNPHNLKLVAVDMDGTFARTDYTYDIPRFKAVLSRMKAVDCQ
                     FVVASGNQYYQLRSLFPNYYDELSFVAENGAFVKDKTEVLFSADMPRKAIEVVLDLCK
                     DYPEVKNVMCGLNSAYCERGKVSQAFFDLTNIYYHRLEWVADFAEVQDKILKFAPTMP
                     VEKTEFYVELFREKLAGLLVPTSSGHGSVDLILSECHKTFGLKMLVERLGINPDQCVA
                     FGDGGNDIEMLEYCGLSYEMDNATEAVKQVAKHQCPSNDEDGVLVTLDRLFPNT"
     gene            complement(1682..2041)
                     /locus_tag="SCL80_002739"
     CDS             complement(1682..2041)
                     /locus_tag="SCL80_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1108565.1"
                     /translation="MSDNLFLEVQPEVFKQVPRLKDGFLLTLNDGSNQFSSAEGCCMI
                     GDHFLLVSTQHYIPAYDLTIPCEEATVKTSEYDTTFINGHLVLEVTPSNQTIRLKNES
                     GILDSNVQVYFDSSDAS"
BASE COUNT          940 a          380 c          481 g          926 t
ORIGIN      
        1 gagtgtaaca taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgttttata
       61 aagcaatcat tcctgagaac ttagatgagg ttaaacttta tttatgggaa gataacggag
      121 aaattgtcgg ttttagtggt gtaagtgcag aagaattagt tatgctattt cttgatccag
      181 attttattgg taagcgatat ggtagtcaaa tattaattga gctgattgaa actgaagata
      241 taaaaagaat tgatgttaat agtcaaaata aatatgcaaa acatttttat ttaagccatg
      301 gatttgaaat tgaatctgaa gaagacttag atggttttgg taaaccatac cctataacgc
      361 atttagtaaa aaaataattc atgaatggtt tattacctga ttgaacatga ataaaagtaa
      421 tatttttatt ttgatggcct atttctaggt tatgaattat ccagatcaaa tttgtgtatt
      481 acattcagta ataaggtgct tatcttagga tcaacttatg atactagctg accaaggatg
      541 taccagttat agaaaatcag ttaaagtaaa aatgataaaa atcttatgtc aacactgatt
      601 taggctgacc tgtgggttag gagaaagaca ggtcggtagc ttttaataca aaaaatggct
      661 tgtatgtatc tagatagtta actgaaatta gaaatgttct tatttttaac ggagtaagaa
      721 aaataggctg ctttaggtct gaatactttt tcaatataat gaagatatca aaggcgacag
      781 gagtgttttt atgaataaga atcctcataa tctaaagttg gtagcagtag atatggatgg
      841 aacatttgct cgtactgatt acacatacga tatcccaagg ttcaaggctg ttttatcgcg
      901 aatgaaagct gttgattgtc aatttgttgt tgcaagtgga aatcaatact accaattgag
      961 atcgttattt ccaaattatt atgatgaatt aagttttgtt gctgaaaatg gtgcttttgt
     1021 taaagataaa acagaggtac tattttcagc cgatatgcct agaaaagcga tcgaggtggt
     1081 ccttgattta tgtaaagact atcctgaagt aaaaaatgtg atgtgtggtc tgaatagtgc
     1141 ctactgtgaa agaggaaagg tgagtcaagc attttttgat ctaaccaata tctattacca
     1201 tagattagaa tgggtagctg attttgcaga agttcaagat aaaattttaa aatttgctcc
     1261 gactatgcct gttgaaaaaa ccgaatttta cgttgaattg ttccgtgaaa aattagccgg
     1321 gctattagta cctaccagta gtggtcatgg ttctgtagac ttgattcttt ctgagtgcca
     1381 taaaactttc ggcttgaaaa tgttagttga gcgcttgggg attaatcctg accagtgcgt
     1441 tgcttttgga gatgggggca atgacattga gatgttggag tattgtggtc tgagttatga
     1501 aatggataat gcaacggaag ctgttaagca agttgctaag catcaatgtc cttcaaatga
     1561 cgaagacggt gttttagtga cgctcgatcg attgttccct aacacgtaaa cactaggtaa
     1621 ttcatcaaaa aacctcgata taagaaatat tcgaggtttt aaaactatat ttagaacaga
     1681 tttacgaagc gtctgatgag tcaaaataga cttggacatt gctatctaaa ataccacttt
     1741 cattctttaa acgaattgtt tgatttgatg gagttacctc taatactaaa tgcccattaa
     1801 taaaggtagt atcgtattca gatgttttaa ctgttgcttc ttcgcaaggt attgttaaat
     1861 catatgctgg aatataatgt tgtgtggaaa ctaataaaaa atgatcacca atcatacagc
     1921 atccctctgc tgaagaaaat tgattagagc catcatttaa agttaacaag aatccatcct
     1981 ttaaacgagg tacttgctta aatacttctg gttgtacttc taaaaataaa ttatcgctca
     2041 ttttcacgac caccatcgac ttccacagtt gaaattgaca ttttatttaa cacacctatt
     2101 cccccttaac tataactcaa ttctatataa aaaatattct attctcaaat aattgaactt
     2161 ttcaaatcgc caatttactt cggctttatc agagctcatt catatcaaga gcgattaacg
     2221 ctgtaaaaat taaaccgaag atctgataaa ttttctctat tttctacaat atttaaaacg
     2281 cttccttagt ctattatata tctatttctc ttaggagtat gtttagatgt ttgaataata
     2341 gttgcccaac tttaaaggtt attcttataa tattttactt cattttccaa tagaaaattc
     2401 aaaggattac tggtttttct aaaatagttt agtttaaata cataagtatc ctccgacact
     2461 gtattagttc ataatcatct aaatacaaaa ttttcaaata aaaatatttc aagctctatc
     2521 gttgacgaat gtaaacgata cttataaagt aatagaaatt gattacattt gtaaacttaa
     2581 aatggttaat ttatctcaca taaaactaag aataaaataa tgattaaaag tggttagttt
     2641 ttatacgatt tagcctatcg gtaaaaatga attagagagt gtaaaatatt ttgtgtaaat
     2701 gaaaaaatcc atacaaaaaa ggaagtc
//
LOCUS       DARBEC010000158         2652 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.165, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000158 DARBEC010000000
VERSION     DARBEC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.165"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            270..1202
                     /locus_tag="SCL80_002740"
     CDS             270..1202
                     /locus_tag="SCL80_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1108566.1"
                     /translation="MTYTHLTTDELVIIESYFKMNQSVAKTAHCLNRSRQTIHKVYLF
                     FKQGKSALEYYQQYKKNKSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPIS
                     CSARTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEF
                     GHLEGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFK
                     SITFDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNS
                     VDEFFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
BASE COUNT          949 a          368 c          516 g          819 t
ORIGIN      
        1 ttccttgaat gttataaaag tatctgtata atgttcagta tattttgatg aacgcatatg
       61 tagtcctcat ttcttgggag ttttgagact actatatgcg tttttttgtt gatttaccaa
      121 catgtttaga cattttcctc taggaaaaaa ttaactagca ccacgggttt ttattacgcc
      181 gattgtaaaa ttaagctaga caactaaaaa agtcatttgt gctacactca aaatagttcc
      241 gaccaaagaa ttataaggag tgagtacaaa tgacctatac ccatcttaca acggatgaac
      301 ttgtaataat agagtcttat ttcaaaatga atcaatctgt tgctaaaact gcccattgct
      361 tgaatcgttc aagacaaacg atccataaag tatatctgtt cttcaagcaa ggaaaatcag
      421 ccttagaata ttatcaacag tataagaaaa acaaatcaaa ctgtggtaga cgtccgcttg
      481 ttttacccga ggaacaatca gaatatattc aaagaaaggt tgttcaagga tggacacccg
      541 atgtgattgt tggtcgtgca gcgtttccta ttagttgttc tgctcgtacc atttatcgta
      601 tgttcaaaaa ggggctattt gattcttctg acttaccgat gaaaggcaag cgtaaaccga
      661 atggacatca agaaagacgt ggaaaacaaa ctttccgccg ctctattcat gaacgtgaaa
      721 aggattatag ccaattctca aatgagtttg gtcaccttga aggtgacact atcgtaggtc
      781 tgaaacataa aagtgctgta attaccttag ttgaacgatt atcaaaagtt atcatcacat
      841 tgaaaccgtg tggtagacaa gcgattgata ttgaaaaaaa attaaatcaa tggtttgaat
      901 ctgtaccgaa aaacctattc aaatccatca cttttgattg tggaaaggaa ttttcaaatt
      961 ggaaacagat cagtaatgtc aatgatattg ccatttattt cgctgatcca ggaacgccgt
     1021 ctcaaagagg cctaaacgag aattctaacg gattgttacg tagagatggt ttattgaaat
     1081 ctatggattt caattcagta gatgaatttt ttattcaatc tgtcgcatct aaacgaaata
     1141 atattcctag aaaatcactg gattatcgaa cgattatcga acacctttgg aagtattttt
     1201 gagttacgta agtattgatg atctgtctaa tttaatttga caattaagaa agtattaact
     1261 tgttttgtga aaaagagaat ttgaaaaacg attggcgaac aaatgctcgg taggtatggt
     1321 ataaatacag agctaattgg tgtgatgaaa tcagatattc gcgtgagatt cagtaaagta
     1381 ggagttcgaa aacagctagg tgacaaacag tattgctttg gtacttcgga atggtttatc
     1441 aaattcttat caacagttca cttctcttta tgtttgagtg atgattcgtt aggttattgc
     1501 ttggaaagac cggatgagag tagaagagtt taattttata aagtgaaaag aggtaatgta
     1561 cgatatacgt agggctgaac caatagagga taagcaaacg atgttacagg cgcaaaattc
     1621 atagcttact gttttggaga taacagaaga ggaaataatg cagaagtaag gcgaaaaaag
     1681 gacggaagcc taacagcata tgaaataaag aaaaatatta taactactga taaatcggta
     1741 ttgagcaaag tactaatagg agtcaaaaac attcataagc tatgagtgtt tcttggctac
     1801 ttttttattt ttatgtatct acaaaagttt ccaggggcag aacctgtgat tattaatgga
     1861 agaacctcaa gtattgtctt aaatcattgt tatgaaaatg gattaaaaga gttcatcaag
     1921 gtgtgattaa caagttactt ttcattacat cgaatttttc agaagttgct tatattggtg
     1981 atgatattaa tgatttatca tgtatgacat ctgtgaagaa gcgggaggaa tagtagaatg
     2041 tccaaaaaat gccacaagaa atgagattga attatctgat tttattgtaa ctcacagtgg
     2101 tggtgacgaa tctgtgatag agtttattga gtggatattg gaatcgatat agtgtagtag
     2161 tacagaaaaa ttatcataca attaatacta ggcataagaa tattggcata ttctagagtt
     2221 aaaagtaact attcgaaaag gataaggtta gaattttttg ttttgccagt ggaagaatat
     2281 aaaaagggca ttggaaatta atatattaga atgctaagcg gagaaaaaaa tatatgaaaa
     2341 gtacgtcctt gtgatgggat ggaaaaatac acttatttca aacaacgata atataattat
     2401 gtgaaccgaa gtatactcgc ttctaattca atcctcaaat cccgacagtc gcgacttcaa
     2461 aaaagcgtat agttttaatg tatccccata tgatccgaaa aatggtaatt tacaattaac
     2521 agtttgatga tgttgaagat gtggaaatgc tgaaattacc atgatgagaa acacattaat
     2581 taaagggaat taattaacta gaatataatc tgattcaatt tgaattaagc aaaaaaaggc
     2641 tgagttttaa at
//
LOCUS       DARBEC010000159         2606 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.166, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000159 DARBEC010000000
VERSION     DARBEC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2606)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.166"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(882..1400)
                     /locus_tag="SCL80_002741"
     CDS             complement(882..1400)
                     /locus_tag="SCL80_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEG1108567.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
     gene            2518..>2606
                     /locus_tag="SCL80_002742"
     CDS             2518..>2606
                     /locus_tag="SCL80_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108568.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVE"
BASE COUNT          858 a          473 c          386 g          889 t
ORIGIN      
        1 atttgttctt ttattaaata cacctacaac ttataaattg atggtattta gataacacga
       61 aagaataaaa ataacagctg atagtaaagt ttgttgacga ttgctactca atcgaaagtg
      121 agttagtgcg tatgaacttg cttctgaaaa cttatgaaat gaaagacctt ttacatcttg
      181 cagaaatcgg tactgggatt aaactaagca ataaaaacta atgttcccct cattctttga
      241 acttctacta tttgatttat ttattctgat aaatgacgat ttcaccttca ggatcttcaa
      301 atcccgtata gaatttattt aagatctctt cagtatcagt aataggatag gcgataggcc
      361 acataccctt tgatggttag aataaactgc tcaaaatctg tatcgtatgt tggctttagc
      421 atattaacaa aggctatctc caaattataa accataagcg ttgaatcgtt cttaacaatt
      481 tcaattcttc ctaacccaaa gtcagaagat ataattaccg gacttgcctt acccttcatg
      541 tgggttgttg agattccata tttaaaggga attgtcggta ctatcggcgg tctgaaaaac
      601 atcccaaaaa taaaaaaatc tttcccctgc tagtctccta agtccctttg tagcggatat
      661 ggcaccataa tctcatttta tcgaagtgag tcatggttta tctgttggcc aaccaaaatt
      721 gatacaatct aacgatgcac cctctacggc attctagggg agtgcacttg cattcaccca
      781 ataaaaaccg gcagttagca aagaacttat gtagtcgtat gttcttactg ttaacataca
      841 agaggtctcc tttccacaat tgcttagcag taaacgggaa tttacaaagc taatctcttc
      901 cattttctta tctttgttct aaaagtccca aatggagcaa ctgtatttac atgaataaat
      961 ttatatactt cccatacagc cgttttggtt gcctcgtctg cccattttct tttgtgtgca
     1021 gtgaatagat cttcatcact cattgaatca atcatcatgt taatatcatc aatattacga
     1081 ttgagaattt cctctaattc tttcaatggt agatgtgcat aggtatttgt aaaccactta
     1141 tataactctc ctaactgatt ccatttaaat tgatcagaag gcgttttaac agataatcca
     1201 tttctttcat cattttccca ttttagaatt aaattcgtcc aaccaacttg atatgctaaa
     1261 ttttcagcag gtgttctatc cacttcctca actcttttat ccttcaattc ttcaggaata
     1321 gtttcaaatt ctaaaatata tttagtataa gtcctattta tttctttttt taattcttct
     1381 ttatttttat atgttctcat tatattacct ccataaattc ctaattgtct cctttaattc
     1441 atctataatt tcatccacga tattttaatc cgttctacaa tgcatatctg atatgcattg
     1501 actgtctaac tcatatgtga atgatttata aaatatccca acgtctcatc taaagaaatg
     1561 ttgatcgtca gcaaggagct aaaaaatacg aatatatttc ttttgtagta aaatcggact
     1621 ggtttgcata tcattttctt cgtaaatatc aaaatcatgt taactactat caattactaa
     1681 taaaaactac cactttaagc gttaagaact gttaagttta gttaagtgag gatagttcac
     1741 aaaatccatt gttaaacttc acgaaagtgg taaaactcaa aatgctttat ataggattac
     1801 agcatcgctc tttcttcgat tgcgcgttgg gtaaaaaata ttatgaagta aaaattgacg
     1861 atgacacgtt ccttacggct catcagatga aacagctaca aaaacgaaat gatcaactcg
     1921 aagaaaattt aattctaaaa aagcgattgc catattcacg cctcgctcga cgaactatta
     1981 aaggtcaaga tttacctgaa gatcatcctt tttaggaaat tgaagctttt tatgttgcac
     2041 aatgcattta taatactaca atgatgtttt caccagatat tcttctaggg tgcggtgtaa
     2101 tgaaacagat acacttgcaa aagaaaattc accgcgaatt tactaaaatt atgaataatt
     2161 atgttaatct tccgaaattt ttttccaaaa ttaagggatc aagctggtgt tatagattgt
     2221 ttaaggttgg caaaaaaata acttcttttt aaacaattat agaaaaccga tactctgtta
     2281 agttggttga cacggtgtcg atttttatga ctttcttatt ctgaaacttc actaacggac
     2341 agtacctaaa aagtctatag tatggacgtt tacgagtact attttatcaa aaatgctgct
     2401 catttcaact tatatgtttt agtgaactac ccccacttag cttggatagg ctacaattaa
     2461 ctagacagaa aaattaaggt gtgtagacta gaagaaaaca taccaggagg aatttttatg
     2521 tctaagagaa cacgaagaac tttttcacaa gaattcaagc aacaaatcgt caatctttac
     2581 ttagctggaa agccacgtgt agaaat
//
LOCUS       DARBEC010000160         2496 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.167, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000160 DARBEC010000000
VERSION     DARBEC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.167"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            72..257
                     /locus_tag="SCL80_002743"
     CDS             72..257
                     /locus_tag="SCL80_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1108569.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALIGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            269..517
                     /locus_tag="SCL80_002744"
     CDS             269..517
                     /locus_tag="SCL80_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108570.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKVTPKEFIEKHLVDL"
     gene            548..727
                     /locus_tag="SCL80_002745"
     CDS             548..727
                     /locus_tag="SCL80_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108571.1"
                     /translation="MGKFNRALVFSAPLIIYALGLWGSRQALIGTIVYMVWIFMGLDE
                     AEAEYRAKKPTEGAD"
     gene            complement(770..1240)
                     /locus_tag="SCL80_002746"
     CDS             complement(770..1240)
                     /locus_tag="SCL80_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108572.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            1327..2025
                     /locus_tag="SCL80_002747"
     CDS             1327..2025
                     /locus_tag="SCL80_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEG1108573.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            2203..>2496
                     /locus_tag="SCL80_002748"
     CDS             2203..>2496
                     /locus_tag="SCL80_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108574.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYG"
BASE COUNT          917 a          358 c          497 g          724 t
ORIGIN      
        1 ttagttattg tcaacgaata ttagctaatt ttttagctta cagattattt ttttagaaag
       61 gagctatttt tatgtctgag aatttagact taaaaattcg agcggagatg agaaaaagaa
      121 gaatgacttt caaagaacta gctgcgctta tcggtatttc aggagcttat ttatcagata
      181 ttctaaacgg caatcgtgat ggaaagaaag cacaacagca tatcgaaaca gtgaaaaaaa
      241 tattggacat ccgatagggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa
      301 aaaatacaat tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc
      361 aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt
      421 atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa aggtaacacc
      481 taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg
      541 aaggttaatg ggtaaattca acagagcatt ggtattcagc gcaccgctaa tcatctacgc
      601 tttaggactt tggggaagca ggcaagcgtt gataggaacg attgtttaca tggtttggat
      661 ttttatgggg cttgatgaag ctgaagctga gtacagagcg aaaaagccaa ccgagggggc
      721 tgactaaaaa tgaatagaaa agagaaacta gaatgtatat tactattact cagtttaatt
      781 ctttcactaa tttctctatt gggaagtttg tatttttgat atcaacaatt ttgtggattt
      841 gggttctatt tagagtaaat atcatttttg tttggtgatt aaggagttta ttaaaattaa
      901 ttttccctac ttcaaatgca aggtaacaag atatagcact tttaggagga atatttagcg
      961 gtacagcttc agagtatatt ctattatcat cgactgatcc tcctttagca attaatttct
     1021 taaatggagt agctgaccat ctgcggttta agttagcgga tgaaagttct aaattaacta
     1081 acatagctgg ttctgttgaa tagttagaaa tgattacttt tgtataaatc atatctgcag
     1141 cgaagtaaga agcattaagt tcaacttcta gttgagggcg cattcctttt atttttatcc
     1201 ctgttaaaaa cgtactcaat ataaaactta ttattgacat ccattgaaaa attgttaagt
     1261 gaaaaaaatt caaaataaac accaccagtt tttaactaaa ttataccaaa aaggagagaa
     1321 gaaataatgc aagaattagt aattttgaaa aataaagaag ctgtgactac gagcttacaa
     1381 gtggcagaaa gcttcgagaa aaaacatcaa catgttttaa gagatattga tgcactaaaa
     1441 aaagatgtgt ccaattttgg acagatgttt gtagaaggta atgaaccaga ttcatatggc
     1501 agaaatcgac gagttttctt cattagtaga gatggatttt tcttgctggc tatggggttt
     1561 acaggaaaga aagctatcta cttcaaacaa aaatacattg aagcattcaa cgaaatggaa
     1621 gatgttattc gcaagaatac tgttcctcaa acaattgaag atatgatgat ctatcaatta
     1681 gaagaaatga aagatgttaa aaaagatgtt tccatgctta aagatactat gcgaattagc
     1741 ggacaacaag agtttgaaat taagcaaaaa ggaaatatga aagttatggg agttctaggg
     1801 ggaaaagaaa gccgagctta tgaagaaatc agcaaaaaag tattctcaaa attttggtct
     1861 gaatttaaac gtaccttttc aatcccaaga tatggcgagt tacctcgtaa gagattcgat
     1921 gatgctgttt catttattga aatgtggttg ccagaaactg cgatccgtat ggaaattgat
     1981 caactgaaca gacaacagag actttttggt gatgacaatg aatagagctg aagcgctaag
     2041 aatagggacg gcaattgcta attgctggtg gaaatactat aaaccaatca tcctaagcca
     2101 acaacatatt gacaagcaaa aagcatggca acaaataaaa aagtgactcc gccggcaagc
     2161 aaagagtcac aaagaaaaca catcataagg agattttagc atatggaaaa agaactttcc
     2221 actctagatc aatatttgat tgatcctgat tggggcaagc cgaaaattga ggaaacaagt
     2281 ggtcgaaaaa tcagacgaaa tcttttgacg aatgaagaac tagcttggga tcaagatgat
     2341 ttaggcaacc atgtaactat ttgggatcat gtttatctta tccatctatc gaagcattcg
     2401 aataaacctg aatatattta cgtcatcgaa gatggcttga ttgatgcgct agaagagtac
     2461 gacagagata acttgattga tatctcttat tacgga
//
LOCUS       DARBEC010000161         2452 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.168, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000161 DARBEC010000000
VERSION     DARBEC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2452)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2452)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2452
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.168"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            148..2301
                     /locus_tag="SCL80_002749"
     CDS             148..2301
                     /locus_tag="SCL80_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEG1108575.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
BASE COUNT          837 a          428 c          543 g          644 t
ORIGIN      
        1 gtaactttaa gtgattgctg tgaaacaaaa agttgatttt atcttgaaat taggtaaaga
       61 atttcttgct ccgcccattt tttctttttt attaagaagt ttttgggtac aatgaagcat
      121 gaatgtaaat aggcggaagg ataggaaatg atgaaagatt ttatgaaaaa aatattaaat
      181 acttcttggt tagaaaaaat aagaaaaggt gcacaagaac ctaagaacaa aacgtttcgc
      241 cggtcccatg ttccttttcg cttaaatttt ctttttttca ttatatttac gttatttgtt
      301 gcgttgattg ctagattggg ttatttgcag atcgtgaacg gcgaagagat ggaggcgcga
      361 gtgaagtcta cttctacgat tacggtccaa gaaagtactc ctcgcggaat gatctacgac
      421 tccacaggaa aagctttagt tacgaataaa gcgaatcagg ccatcacata tacgagagga
      481 acacaagtta cggctaaaga tttgcttgaa atcgcaacga agctgaactc gctgatcgat
      541 gttccggtag atgaaaatct gacagataga gataaaaaag atttttggct ggctgatccc
      601 aaacatttga aagaagccac aaaaagactt tcggcaaaag aaaagcaatt agggaacagc
      661 gaacaatata gtgcgatggt caataaagtc acagctgatg agattaattt aaacgaggag
      721 caattaaaaa ttgctaccat ctttaaacgg atgaatagtg catatgaatt aaacactgtc
      781 tatatcaaaa actctggtgt tactgatcaa gagttagctg ttgtggcaga acgcgcttct
      841 gaacttccag gagtttcgac tggcacagac tggacacgag aatataatgc agcaagttca
      901 ctgaaaagta ttcttggcag tgtgacgact gaaaagcaag gacttccagc agatgaagca
      961 gagaaatacc tagcaaaagg ttattctcgg aatgaccgtg taggacaaag ttatttggaa
     1021 aaacaatatg aagatgtcct gcaaggaacc aaaacccaat atgaagtttc ccttgataat
     1081 gaaggaaatg tttcgaatca aaaagaaatc ttctctgggg aaaaaggttc taatttaatg
     1141 ttatctatga atgcagaatt ccaatcaaaa gtagaagaaa ttttgaaaag gaattatcaa
     1201 acattgatca acaacggcaa agcacagtat tcgcctggtg cttatgcagt ggctatgaat
     1261 cctcagacag gagaagtgct agcaatgact ggtttcagtc atgaacaagg ttccaaagaa
     1321 atcaccgaga atgctttagg cacgattact agcgcctttg atccagggtc tgtagtcaag
     1381 gctggtactt tgacagcagg ctgggcaagt aacgcgatca gcggaaatca agttttgatt
     1441 gatgagccga ttcgtctgca aggtgcttca gaaaaaagct ctgtctttaa tcgaagcgga
     1501 caagttgctc ttgatgcagt caaagcatcg gaattgtctt ctaatacgta tatgatcaaa
     1561 gtggcgttaa agatgttagg actagactat acacctggaa tgggactacc tagtttagat
     1621 gaagaagcta aagcttatca gcaattgaga gactctttca aagagtttgg tcttggtaca
     1681 acgactggga tcgatttgcc gaatgaatct cctgggatct cacgttctgt tgattatatg
     1741 aagaagttca atgcggataa tggcaaagaa tggtatgctc caggtaactt taccgatttg
     1801 gcatttggac agtttgacac atatacacca atccaattag ctcaatatgc ttcaacagtc
     1861 gctaatggcg ggaaacgagt acagccgcac ctagtcaaag cgatctatgg caatgacgaa
     1921 aatggaaact tgggtgaagt gaagaaagaa attggcacaa cagtagaaaa tacggtcaat
     1981 atttctgcag aaaatatggc gatcctccgt gaaggattcc accaagttgt ccatggtacc
     2041 gatccttaca caacagccaa accattagcc agtgcgaaaa tggacctttc cgcaaaaaca
     2101 gggactgcag aaacggttgc ggaaggtcat ccagatattg cgacagtcaa tagtaatatc
     2161 gtggcatacg gaccaacaga caatccggaa attgccatca gtgttgtctt gccgaactta
     2221 ttagacgaac aagaccacat aaacttgacg attgctaaag aaataatgga cgcgtattat
     2281 gatatgttca tggcaaaata aaaaagaggt cgtgaaaaag ccgcttagtt tcgagtatga
     2341 agaaaaattt tgtgaaaata gcttctcaca tttttgctta aatttgactt cataccgaga
     2401 aactggcttt tgaacaccgt ttactcggca aaaaaggttg tgacaaaaat tg
//
LOCUS       DARBEC010000162         2436 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.169, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000162 DARBEC010000000
VERSION     DARBEC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2436)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2436)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2436
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.169"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..272)
                     /locus_tag="SCL80_002750"
     CDS             complement(<1..272)
                     /locus_tag="SCL80_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108576.1"
                     /translation="MINLGEWGMIAGSITAIVSLILLVIRPIAASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKT"
     gene            complement(309..446)
                     /locus_tag="SCL80_002751"
     CDS             complement(309..446)
                     /locus_tag="SCL80_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1108577.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEADKIINKDKSA
                     S"
     gene            complement(448..894)
                     /locus_tag="SCL80_002752"
     CDS             complement(448..894)
                     /locus_tag="SCL80_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108578.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGVRSDGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYEALEHENGNLKQLNAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            complement(891..1040)
                     /locus_tag="SCL80_002753"
     CDS             complement(891..1040)
                     /locus_tag="SCL80_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108579.1"
                     /translation="MKVVYKSIKPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            complement(1054..>2436)
                     /locus_tag="SCL80_002754"
     CDS             complement(1054..>2436)
                     /locus_tag="SCL80_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108580.1"
                     /translation="GKPNLMSKLKSSDFNVGYHGSLTSDNEKLHFTSDGTGSIIMFTR
                     INTPQLASGKTYTLSAKVRFDEGTTGAIDKLRLVYRTSPGEKILLEANSTNITTDDVG
                     KEITIKGTANVNYQITNLDRFYMSISFVDRDKINGGFKLYDIKIEEGSTATPYQPNLL
                     DDPYWVGKTSLGENIANKGTTFPIKSSAYEIYKGNTEEELIIGQTYTITLKGTKPASQ
                     TFVAYNYWNVNFGELKPVEGLTDVWSLTFTPTKLEPGLPKDLRIFQLPKETAGACQID
                     WLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDITPEYTEKGLNDTVSLTEPQSI
                     EGLKNFEDGIQSKGKSVLTSDDNKYEVVTLTVTNGNTGSAKLYREGKTVTIYFFALNG
                     KSSGGNDSTILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGS
                     AYSFVITYSI"
BASE COUNT          638 a          502 c          420 g          876 t
ORIGIN      
        1 aaagttttga tttgttgcgt gtgttccacc aatttttctg tatgtgtatc taatctttca
       61 tcgtgtttct ttagttcttc atgaatcatc aatcgatctg atttgctcga ttctaaatct
      121 ttagtcagca aatctaaatt gtggcttact tttgaaagag tctcagtaat tttcgagaaa
      181 gatgcagcaa tcggtctaat tactaataaa atcaaagaaa cgatagcggt tattgatcct
      241 gctatcattc cccattcccc taaattaatc atgtgacaac tcctttacct taaataaaaa
      301 cgcatcaatt aagatgcgct cttatcttta ttaatgattt tatctgcttc ttcgtctgta
      361 atgcatagtg gaacgaatag tcgaacttga tcgtcagtaa aacagcccca atcatacatc
      421 attttcacat cgctaaaact aaacatacta ctcacctcct tttgaagctg gatttagttg
      481 ctctttaatt tctgaaatgt ctttgctatt ttgtaacgaa gcaagcatca tttttgaatt
      541 gatttgtgct aaactgtcag ctttttcttt caatgcagta ttttcctgtt taatcgctac
      601 atcgcttagc atgagtttgg catttagctg ttttaggttg ccgttttcat gttcaagagc
      661 ctcgtacatc gctttgagat tgtttaaatc gttgtgatct aacgcgttcg ctaaaataat
      721 ccattggttc aatttaggat caaacatctg atcagcaatc gttagcggtt cgccatctga
      781 acgaacccct tcgagcggtg gctgatctgt gtaaggaacg gatacaagca tgtcgtccaa
      841 tacttttcct gcatactctc cgccagtacg gccatatttc caaatgtttt tcatttattt
      901 ccctcccagt aattgaattt cggtttccaa tttggaatcg gtggttcgac ttctgtgcat
      961 tcttccggta aatgttcttc gtcattcaca atgatttgct cgaatccgta aggcttgatt
     1021 gatttgtaaa ctactttcat tagatttcct cccttaaatt gaataagtga taacaaatga
     1081 ataagctgat ccataacttg agtttcttcg ccatttaatg gctccatctg caccaataga
     1141 taactgagca ctgttcaaag tagaacggtc tatcgagcca accagttgct caaaactaat
     1201 tggtggccga tagccttctg gaattgttag tatcgttgaa tcatttccac cactgctttt
     1261 cccattcaac gcaaaaaaat aaatagtgac ggtttttcct tcacgataaa gctttgctga
     1321 tccggtattc ccgtttgtaa ctgttaaggt tacgacttca tatttgttgt cgtctgatgt
     1381 caatacagat tttccttttg attgaatacc atcttcgaaa tttttcaatc cttcgatgga
     1441 ttgcggttca gtcaaactaa ccgtatcatt caagcctttt tcagtatatt caggcgtgat
     1501 gtcccagctg tagtcgttcg gattgttgct atctttcaat ccttcaccaa agtatttaaa
     1561 ctgactaata ttcggggttc gggtgtcgcc tttttcaatt ttgagccagt caatttggca
     1621 tgcgccggcc gtttctttag gcaactgaaa aatgcgaagg tctttaggca aacccggttc
     1681 aagtttcgtt ggtgtgaatg ttagagacca tacgtctgtc aatccctcaa ctggttttag
     1741 ttctccaaaa ttaacgttcc aataattata tgctacaaag gtttgacttg cgggttttgt
     1801 tcctttaagc gtgatagtat acgtttgacc tattataagc tcttcttccg tgttaccttt
     1861 atatatttcg tacgcgctag atttaattgg gaacgtcgta cctttatttg caatattctc
     1921 acccaacgat gttttaccta cccagtaagg gtcatctagt aagttaggct ggtacggtgt
     1981 ggctgttgag ccttcttcga ttttgatgtc gtacaactta aatccgccat ttattttatc
     2041 cctgtcaaca aagctaatac tcatataaaa tcgatctaaa tttgtgattt gataattaac
     2101 gttagctgta cctttgattg ttatttcttt ccctacatca tctgttgtaa tatttgtact
     2161 atttgcttcc aataatatct tttctcctgg tgatgtacga tacaccaaac gtaatttatc
     2221 aatagctcct gtagttcctt catcaaatcg aactttcgca ctcagagtat aggttttccc
     2281 actagcaagc tgaggtgtat taatacgcgt aaacataata atgcttcctg taccatcaga
     2341 agtaaaatgt agcttttcgt tatccgaagt tagtgacccg tggtaaccaa cgttaaaatc
     2401 gctcgatttt agtttggaca ttaaattcgg cttacc
//
LOCUS       DARBEC010000163         2408 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.170, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000163 DARBEC010000000
VERSION     DARBEC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.170"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(243..464)
                     /locus_tag="SCL80_002755"
     CDS             complement(243..464)
                     /locus_tag="SCL80_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108581.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(562..807)
                     /locus_tag="SCL80_002756"
     CDS             complement(562..807)
                     /locus_tag="SCL80_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108582.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1047..1661
                     /locus_tag="SCL80_002757"
                     /pseudo
     CDS             1047..1661
                     /locus_tag="SCL80_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2031..>2408)
                     /locus_tag="SCL80_002758"
     CDS             complement(2031..>2408)
                     /locus_tag="SCL80_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1108583.1"
                     /translation="PIEFDNDERLLLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRS
                     YLSDRVFDVLEKLSKSGILKEEYDSSFDEYPSRYILISEDYKKFKRYFLKNEKKISVI
                     MEKYKAPIQADPFSNLDESDLPF"
BASE COUNT          816 a          419 c          332 g          841 t
ORIGIN      
        1 actatatact tcttcccaga acttgtccgt ctccttcaaa ttatctctac ctctcgagtt
       61 cattgggtgg tttgcccatt tattaaacat ggaatcacct ttctcatact catatattta
      121 tgtcctattt caattatatt tatatatgct taaataaatt tgtatcctga aatttattgt
      181 aatatgaagc tgtattttac atatagtaac caaagagtta ataaaataaa aagagcgaac
      241 cattatagat cagctctttt ttcattgctg tttttaatct tatctagaat cacttcaaat
      301 gtttcaaagg tatttacatc taactgacat aaccaaagca agtacttctt ttctttgtaa
      361 gtccaatcaa tattacggta agaggacatt agattatggt accttgctct ttgttcaact
      421 gaagccaatt cccagcgttt ttcaaatatt ttttcttctt ccattattga tccctcgcaa
      481 gacgctacct caaacttaaa taatgggcag cgaaagtcta attccttaga tctaagtata
      541 gcaaacaaat atgaaaaaaa cttagaaggt agtaaaagtt cttccgtctt ttgctttgct
      601 tctattctta atgtgtttcg ttatcggtgc cctacaaaga ttttcattaa ttacacactc
      661 atttattgtt gtgtattttc tgacatttct ctcttgatca attattttaa ttccatgggc
      721 atcctgatag ttcataggag aggagcggct atcagaaata gttgcaccta tctcttgaac
      781 ttcttttaca acttctggaa aatccatcca tcctgcttca gctgtcaaaa gtaataatct
      841 ataatattta tctttccttt tcaatctaag aaatcgtcac cccattcttt ttcttctatt
      901 ataaactatt taacttttag aattcaaaaa ccgtttgtct atttagagtt taccctaaat
      961 agacaaaaat ataacattga aaaatttaga gttctaacgt atgattaggg tgtattataa
     1021 acgttataga aaagtaggga ataaatttga aaattgctta tattagagtt tcatccatcg
     1081 atcaaaatga acaacgtcaa attgaagaaa tgaaaaagtt tggcgctgaa aaaattttta
     1141 tcgaaaaaca atctggagcg acgattagtc atcggccgat tttccaggaa gctttagatt
     1201 ttgtcagaga gcaagatatt tttattgtag aagccattga tcgacttgga cgcaattatg
     1261 acgagattat cgattcagtg aattatctaa agaagaagaa tgtgcgactt attatcacca
     1321 gtttaccgat catggctgaa gcaattggaa atcctctcct agataaattc atcaaagact
     1381 taatcattta aatctcagcc atgattgctg aacaagaacg tactgaatcg aaacgacgcc
     1441 aggcacaagg aattaaaatc gctaaagcaa acggcgtcta taaaggtcgt cctaagttat
     1501 acagtgcaga caccaaagat cctcaacgtc gccttgtcta taggagtatc gttcaggatc
     1561 ttgagaatgg ggttgctatc tcaaagattg caacagacta caatgtgact aggcaaacaa
     1621 tttaccgaat aaaaaaagaa atcgaccaac taattgtatg atttcttttt tgaatcacgt
     1681 aattattaat tagctttgtc atcaatacag acaatcattt tagattcatc tagctaagtt
     1741 gattacattt atgttaaaaa gaatttagtt aacataaata taattgatgc ataaatcgtc
     1801 cttatttaaa ggcatttaac attgacaact tagtataatg tatcttatgt aaactaataa
     1861 gatttgaatt ttattttata cacactcgat aatagatcaa ttttatggtc ccccgcattt
     1921 acgattgcac aagagaaaca taaagtcgac ctattagcat ttcttttcaa ttcaaacatt
     1981 aaaaaagttt atacctctgc tactaaaaaa tgaaattatc tatttaaaat ttaaaacggt
     2041 aaatctgatt catccaaatt ggaaaatgga tcagcctgta ttggagcttt gtatttctcc
     2101 ataataacag atatcttttt ttcatttttt aaaaaatacc ttttaaattt cttatagtcc
     2161 tctgaaatca aaatatagcg tgatggatac tcatcaaaag aagaatcgta ttcttccttt
     2221 aaaattcccg atttacttaa tttttctagt acatcaaata ctctatcact caagtaactc
     2281 cttaaattgt tatcactttc ccattgaact acttttctat aagttgaatt atcagttgaa
     2341 aagatagaat tttcatccat aaacgcatat attagcagca atctttcatc attgtcaaat
     2401 tctattgg
//
LOCUS       DARBEC010000164         2407 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.171, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000164 DARBEC010000000
VERSION     DARBEC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2407)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2407)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2407
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.171"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            5..1150
                     /locus_tag="SCL80_002759"
     CDS             5..1150
                     /locus_tag="SCL80_002759"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEG1108584.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="SCL80_002760"
     CDS             1321..1779
                     /locus_tag="SCL80_002760"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEG1108585.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..>2407
                     /locus_tag="SCL80_002761"
     CDS             1938..>2407
                     /locus_tag="SCL80_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEG1108586.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
BASE COUNT          802 a          452 c          455 g          698 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttcttta
//
LOCUS       DARBEC010000165         2398 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.172, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000165 DARBEC010000000
VERSION     DARBEC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.172"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            207..2069
                     /locus_tag="SCL80_002762"
     CDS             207..2069
                     /locus_tag="SCL80_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1108587.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="SCL80_002763"
     CDS             2069..>2398
                     /locus_tag="SCL80_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1108588.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DARBEC010000166         2354 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.173, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000166 DARBEC010000000
VERSION     DARBEC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.173"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            451..1944
                     /locus_tag="SCL80_002764"
     CDS             451..1944
                     /locus_tag="SCL80_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEG1108589.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            2079..>2354
                     /locus_tag="SCL80_002765"
     CDS             2079..>2354
                     /locus_tag="SCL80_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEG1108590.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRK"
BASE COUNT          807 a          368 c          479 g          700 t
ORIGIN      
        1 caaaatgtca aaatatattt gtgcgttttg cgttgacaat ttagagtgaa aagaggtaaa
       61 cttaattcta tagaaaccac agataattca ctatttacaa agtagcaaaa gcagtggtac
      121 tctttaaaca gaaaaattac ataaaaaaaa gagacggaaa taattgattt tagcagaatc
      181 aattatttct gaatcacatg gttaggacat gtgttcacgc tcgtctcaag aatatgtaag
      241 tagctaccaa ctacttatat taaaagttga tcggatacct tttccgattg cctttattct
      301 accaaaaaaa ggcgagcaac tcaacagaag aacgtttatt tagtgtactt aaattaaata
      361 ttcttaaaca ctgaagagag aacacatact atggctgaga ttaaagctaa actgtgttct
      421 ctttttgttt gagaaaggac gttacaatcg atgttgagcg aaatttattc agctattctt
      481 aaaaacggct tacgtacata caaatatcga aacagcacca tgaaacctgc aggtcatatt
      541 gattccagta ggagtggtgc tatctttgcg taccgtagca agcaattaat gaatgttggg
      601 cgtggtatgg tcattacttc ggaagaagct attttagaga acgaaaagaa actaacgcat
      661 tggacaccta atacctatcg ctttggtact tatgcggatg attatcgaac agtggttaaa
      721 ggtcattctg agaaaaactt aagtcaaatc aacacatttg tagttgatat cgattcaaaa
      781 gagaaccatc aaggcgaaat tattttagcc tgtttagatc aagtgggcta catgccaacg
      841 ctaattttag aatcagatca tggttatcaa gtttattttg tactaaagac accaggctat
      901 gtcacaaaga aaagcaattt caaggtgatc gatgtagcaa aaaaaatttc aaaaacaatt
      961 cgtctacagt tggctgaaaa attgtccgga gtggatctag gatgtaacca cttcggaatt
     1021 gctcgattcc cacaagaaaa taatattgtt tttaaagaaa tgaactatca gtattctttt
     1081 gctgactgga ttcaatggtc gatgaagctt gaatcagatc aaaaaacaaa agaagatcga
     1141 gaacgaaaac taattgtctt tcctgagaaa aaagaaattc gtcaagtaga tgaaccgtgg
     1201 ttcgatatgt tacttcataa agcaaacatc gtcggcggag aaggacgtct tggtcgaaat
     1261 aatgtgattt tcacgctttc tttagcttat tactcttcag gttatgcatt ggatacttgc
     1321 gaatacaata tgttcacatt caacgaacgg ttacaggaac cgctatctga agcagaatta
     1381 gtaaagattg tacgtagtgc ttattctggt aattatcaag cagcaagcag agaattcatt
     1441 attgaattgt gccagcaatg ggtttcacca accttaaaaa ataatgatct gttcattcaa
     1501 cgacgtggct ggtggaaatt taaaaagcca cgagatcaaa gaaaatatag tcataaaaat
     1561 gaatggaaag aagatctatt ggcttatcta agcgaaaaaa gtcttgctag tgagccttat
     1621 ttgaacctgt caaaaaaaga attggcttca caactaacga tgcccaagcg aacacttgat
     1681 aagatcttaa atgagttacg agaggagaat aaagtcttct acaaggtaac acgaggaaga
     1741 aatggcggaa tgatcattgc ttcagtcaaa acgttgttcg cgagggttat tcaactaagt
     1801 aaagaaaaga gactagatta ttcaacaagg atcacagagg tatttggact caacgagagc
     1861 ttagtagaag agacatttag acaattatca acccccttga accctcttaa agaagtgaat
     1921 ctttttgaag tagatacagg ttgaaaaata aaaggtgcaa attgccttat acaatatatc
     1981 tatgatactt ctgtaggtgt tgctcccttt gcctagctac gtgtaggtgg tatacttata
     2041 actgcgtatg cagtcttatg tatcatggag gaagttgtat gtcgttaaaa aattatgtct
     2101 attcacatcc tgttaatgtg tttatcattg gtcgtttggg attatctgtg gaagtgttct
     2161 gtgagcttta tggttttaag caggggactt tgtcttcttg ggttaccagg gaaaaaacag
     2221 ttgcttcttt gccgattgag tttcttcatg ggttgtcctt agcttctggg gagactatgg
     2281 atcaggtata tgattgtttg tgtgtgttag agcaggagta tattgagtat aaaatagcta
     2341 acgagttaag aaaa
//
LOCUS       DARBEC010000167         2349 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.174, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000167 DARBEC010000000
VERSION     DARBEC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2349
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.174"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..557
                     /locus_tag="SCL80_002766"
     CDS             <1..557
                     /locus_tag="SCL80_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1108591.1"
                     /translation="NIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAFKS
                     MGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIEIH
                     LDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFFEN
                     VVTGKENWNDLYHANEVLKIAEEK"
     gene            558..1310
                     /locus_tag="SCL80_002767"
     CDS             558..1310
                     /locus_tag="SCL80_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEG1108592.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            1315..2262
                     /locus_tag="SCL80_002768"
     CDS             1315..2262
                     /locus_tag="SCL80_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEG1108593.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT          833 a          301 c          454 g          761 t
ORIGIN      
        1 aaaatatcat ggaagttgtt cgtaatagta ttcaaagtaa aaaagtatat tctgtaagag
       61 ctatttgttc tacttatctt cctgattgga gaaaaggtac tgattataga aataattatt
      121 ctgcctttaa atctatgggg ggaggagtag aattagactg tatacatgaa ttggattata
      181 ttataaacct atttggtttc cctaaatcag taaaaaaaca gtttggtaaa tattcgaatc
      241 ttgaaattaa cagtaatgat ttagcaacat atatattgag atacgatgat aaaattatag
      301 aaattcattt agattatttt ggaagagttc ctacaagaaa atttcaattg tttactttag
      361 atgaatgtat caatgttgac ttattaaata ataaaataag ttttgaagaa aacattgtta
      421 aagaaatgcc tgaatctgct aatgatatgt atgagaagga gttgaaatat ttctttgaaa
      481 acgtagtaac aggcaaagaa aactggaatg atttatatca tgcaaacgaa gtattgaaaa
      541 tagctgagga gaaataaatg gaaattttat ttactatttg tggtagagct gggtctaagg
      601 ggtttaaaaa taaaaatttg aaatatttgg caggaaagcc cctagtccat ttcgctttag
      661 caatgataga tatctatcaa caaaaacatc ctgaacataa ttgcacgact tgtttgaata
      721 cggacagtaa tgagttagta gaattgacga ctaagttaaa tccgtctgtg aaaattgtag
      781 aacgtaaacc tgaattagct acagatactg tagggaaaat tgaagtgata cgtgattcat
      841 tcttacaact agaaagagaa gataatatat ttgatttaat tatagattta gatttgactt
      901 cacctttacg tgaagtttct aatcttgaag aattaataga tgaacatcta aaaaatttag
      961 ataaagacgt ctacttttca gttgtttctg caagacgtaa tccctatttt aatatggtaa
     1021 aaagaattga aaatgaagtt actatagtta ataaatcaag atacacatct aggcaacaga
     1081 cacctgaagt ttttgaaatg aatgcctcaa tgtattcttt taaacctgaa tttctaaaaa
     1141 aaagtaatta tatttttgat gggcaatgtg ggataataaa aatgagagat tatctaattt
     1201 tagatattga tagtgaagaa gattttaaat ggttagaatt tttgtttcct aaatttttag
     1261 aagattcttc ggagcttcaa gatatctata attatttata tcggaggtaa aaggatgagc
     1321 tgttatctta tcaccggtgg ggctggcttc attggttcta ccttagccaa tcatttagga
     1381 gaagaacatg tggtaatcgt cgtcgatgac ttatcaatgg gaaaagtaga aaatcttgat
     1441 atgacgaaaa atatcacttt tattgaggga gatgtcgctg agaaatcctt gatggaagag
     1501 atcatgaaag ctaataaatt tgattatatc tttcatttag cagcagttgc tagtgtggca
     1561 gattcagttg cccgaccagt agaaacacat cgtgtgaatt ttgaaagtgt attaatgatt
     1621 ttagaattga tccgcaagta tcaacctgat ttaaaacgga tcgtgtttag ttcttcagcg
     1681 gctgtttatg gggatgaacc gactttacca aaaaaagaag agtccgtgat tcgcccattg
     1741 acgccttatg cggttgataa attcgctgca gaacaatatg tgctagatta ttgtcattta
     1801 tatgatgtac ccggtagcgc agttcgtttt ttcaatgtct atggtccaaa tcaaaatccg
     1861 aattctccat acagtggtgt gatttctatt ctcgtagatc gctataagaa acaaatcgta
     1921 ggagaagcaa cttcatttac attatttggt gatggaagcc agtctagaga ctttgtttat
     1981 atagatgatg taatccaagc attactttta gttgcgaatg aggaagctgc actggggaaa
     2041 caattcaatg ttggaacggg taaagcaacc acacttttgg aattgattca tgcaatcaat
     2101 gaaattctcg gtgtagaatt gacacttgaa tacaaagaag agcgttcggg tgatatccat
     2161 gactctctag cagatattac taagatccag tcaattggct atcaaccgaa atatggtatt
     2221 ttaagtggaa tgactaatta tttgaaaact gaaatcaatt aaaacatact aagataagat
     2281 tagagggtaa attcaggctc tttgtcaata aggactgatg agttttgtaa aatcaaatct
     2341 gggatagaa
//
LOCUS       DARBEC010000168         2325 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.175, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000168 DARBEC010000000
VERSION     DARBEC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2325
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.175"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..1211)
                     /locus_tag="SCL80_002769"
     CDS             complement(<1..1211)
                     /locus_tag="SCL80_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEG1108594.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLDDTIALHQRKLDLL
                     KETKKGFLQKM"
     gene            complement(1201..>2325)
                     /locus_tag="SCL80_002770"
     CDS             complement(1201..>2325)
                     /locus_tag="SCL80_002770"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEG1108595.1"
                     /translation="IKKLNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHM
                     VSDMMAQIVTLDQKERRFFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYN
                     LAKMNLILHGVDAEEMNLRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDD
                     SRFNRYGKLAPKSKADFAFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLED
                     GSIYAVIGMPANLFFGTSIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENI
                     QKILETYAERKDVEKYAHLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKK
                     IRQEQQVLEKELLEAISSLQTTPENEAWLQGALEVFKHEQ"
BASE COUNT          650 a          495 c          383 g          797 t
ORIGIN      
        1 aacatctttt gcaagaagcc ttttttcgtt tccttcagca aatctaactt acgttgatga
       61 agagcgatcg tatcatctag ctgcttgaag aaggcaccaa ttttttgttg ttcatttata
      121 tccggtggaa actgaaatgt cctttgttct ataccacctt tagtcacatg taccattgtc
      181 gttccgttag ttgattcttt aattttctct ttatcagtca atagccaagt atatagatac
      241 tgtttgtcca attccttaga taaaaattta agtttccaaa tatgataatg gtagattaca
      301 cgttcttcat tccacatttc aggaccgaag cttgttgccc ataaatataa gagatctcct
      361 ttattagcat acttgtcttc ctctaattct aagtttgaat aataccatct atcattagta
      421 ttaaaattac ctactcttaa aaccctatat tttccagaat caagtaattc ttgttgttta
      481 aaagctctac cattcaaaaa aactaccact tcccccaact tccgctcttc ccaatctccc
      541 gtaaatcctg gaaaacgaac ttctggcact tttgctccgt ttttagggaa cattttttgt
      601 aagaatcctt ttttcgtttc cttcagcaaa tctaattcgt gctggtgaag catgatggtg
      661 tgatcaagtt gtttgaaaaa tgaaccgatc ttgatctgtt cgtcataagt tggaagtgag
      721 ataggtgtct ttttaccact atcaattgta taggttccga cggttccaat gcctgcaacg
      781 tcacggatat acttctgaat tttgttattt atgaacgagt agtaaaagaa gtttccgtca
      841 attttatgct cattcttgaa ccaaatgatg tttccatcct taaagtaaat tgggttatca
      901 tcagtgatta ataaggggac acctattgat ccgacaccag ttacaagcaa gtctccttga
      961 gaaactttac ctgaattttt cgaatattca ttatattttt cttctgaaat gtaaagataa
     1021 tcggatggtt cctcattttt tgcagctgag acaatatcac gagcacgtaa aaatctaata
     1081 cctgagttag tccaatctga ttgatgaatt cgcttcacag aagtaacatc catcatatct
     1141 cctaacttac gctcttccca atcttccgta aaaccaggaa accggatttc tggtcgtgta
     1201 tcattgctca tgtttgaaca cctccagcgc accttgtaac cacgcttcat tttcaggtgt
     1261 tgtttgcagg gaagagatag cttctaaaag ttctttttct agcacttgct gttcttgtcg
     1321 tatcttcttg atgtcatttc cgacatgaac catatcaatt ggttcttctt cttcgaaagt
     1381 atcgacatag cgtgggatat ttaagttata gtcattttct ttgatttcat caaaggttgc
     1441 caaatgagcg tatttctcga catcttttct ttcagcgtag gtttcgagga ttttttgaat
     1501 gttttcttcc gataatttat tctgattttt ccctttaaca aattcacgac tagcatcaat
     1561 gaataataca tctcgagttt gacgattctt cttcaataca atcaccgtcg ttggaatcga
     1621 tgtaccaaag aacaagttcg caggcatacc gatcacagca taaatactgc cgtcttctaa
     1681 taatttttga cgaatgaccc cttctgcagc accacggaat aaaaccccgt gtggcaagac
     1741 gatagccata gtccctgttt ccttcaaatg atagaaacca tgaagaagaa acgcaaaatc
     1801 tgctttggat tttggcgcta atttcccgta gcgattaaac cgagaatcat ctaagaaggt
     1861 cgtatctgct gaccaattag ccgaataagg tggattcatc actactgcat caaatgtgta
     1921 aggttcatct gttggccagt ccttattcaa cgtatcccca ttgcggagat tcatttcttc
     1981 ggcatctacc ccatgtaaaa tcaagttcat ttttgctaga ttgtatgtcg ttgtatttag
     2041 ttcttgccca tgatatttaa cgttatctgg atgagttaag tagttacgta catttaacat
     2101 caaggaacct gaacccatgg ttgggtcaaa gacactaaag aatcgtcgtt ctttttgatc
     2161 caacgtcacg atttgtgcca tcatatcaga aaccatatga ggcgtgtaaa attctccggc
     2221 tttcttccct gcttctgaag caaattgact gatcagaaat tcatacgcat ccccgatcac
     2281 atccccatcg tgacctaaaa cttccacgtc attcaatttc ttgat
//
LOCUS       DARBEC010000169         2234 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.176, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000169 DARBEC010000000
VERSION     DARBEC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.176"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..544
                     /locus_tag="SCL80_002771"
     CDS             <1..544
                     /locus_tag="SCL80_002771"
                     /inference="COORDINATES: protein motif:HMM:NF033516.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1108596.1"
                     /translation="ISKENLLNQDFSTETICEKWAADITYIPTKKNGWCYLSSIMDLH
                     TKRIISYTFSKRMTVDCVIQTLNKAKIHYHIPEGMILHTDLGSQYTAREVEQWLKTNK
                     IRHSYSRKGTPYDNAGIESFHASLKKEEVYTTSYSDFEEANRALFSYIEGFYNRNRIH
                     SSIHYLTPQEFEELAKEKMA"
     gene            complement(900..1472)
                     /locus_tag="SCL80_002772"
     CDS             complement(900..1472)
                     /locus_tag="SCL80_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108597.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENCVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(1488..2093)
                     /locus_tag="SCL80_002773"
     CDS             complement(1488..2093)
                     /locus_tag="SCL80_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEG1108598.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
BASE COUNT          701 a          454 c          339 g          740 t
ORIGIN      
        1 catttcaaaa gagaatctct taaatcagga cttttctact gaaacgatct gtgagaaatg
       61 ggcagctgac attacgtaca ttcctactaa gaaaaatggt tggtgttact tatcttcaat
      121 catggattta cacacgaaaa gaattattag ttatacattt tcaaaacgaa tgactgtgga
      181 ttgtgtcatt caaacattga ataaagcaaa aatacattat cacattccag aaggaatgat
      241 tctacacact gacttgggca gccaatacac agcaagagaa gtggaacaat ggcttaaaac
      301 caacaaaata aggcattctt atagtagaaa aggaacacct tatgataatg ctggaatcga
      361 atctttccat gcctcattga aaaaagaaga agtctacacg actagctact cagattttga
      421 agaagcaaat cgagcgctat ttagctacat tgaaggattt tataaccgaa atcgaatcca
      481 tagctcgatt cactatctaa ccccacagga atttgaagaa ttagcaaaag aaaaaatggc
      541 ttaaccgtct ctactaaaga caatcaacac cgtagggata tggagataaa atcaatagag
      601 tcaatagatt atttatcgat aataacacta aaaaagccac gttcctaaac gtgacttacg
      661 ctgaacgata tgtgttgttt ggtagacgaa caaatcgttc attataaata tatgatggta
      721 tcctaatata gtaagcgtgg ctagcatacc tttaattact ttacctttta cagttaccat
      781 acacattttt tgagcagaaa tcaagtaaat aaaaaaatat tatatttcac aacatgctaa
      841 tagactcata tctagaacgc attaagtatt tacctgtctg gttcgctgag acttcttttt
      901 taactttctt caattttctt ctttattcta aaaagcgtac tgcgactgaa accggttata
      961 gcttcaactt ccttatacga tttacctgaa gtaagcagtt catatgcgtg cctcttcttc
     1021 ggtgtgattg ttgctttcgg acgtccttct ctgaaattag gattattctt tcttgcaaac
     1081 atttttcctt cttgggtacg ttccacaatc atatcacgtt ccatttctgc tacactcaac
     1141 aacgtccgtg tcaccattcg ccccatggac gtattttcaa tcgttcctag attcaaaacc
     1201 ttgatcgtta cgttatcatc gagtaaaggt tcaatgatct ctaatgcttc tctcgtgtta
     1261 cgagcaaatc gatcaagctt cgtaacaact atttcatctc caggcttcag accagccaac
     1321 agctgattga attttggccg atcccgtttt gtacccgtaa atttttctga aaaaatattc
     1381 ttcttctcta cgcagttctc aagcaactgt tgaatttgtt cttttgtact ttgagaagcg
     1441 gttgagacac gtgcatatcc atatttcgtc atttggtcca cctactttta caaatcatct
     1501 atcgtatact cagaataccc ttcatagaaa tagctgacat cgattccttt catatataac
     1561 tcgcgatcgt caattttatc agtcagagca ttggaaatta gatagcgtat ttccaaatcg
     1621 tttatgggac ttcgctccat tgcagaaaga tagtctgctt tatcgattaa gttccaatct
     1681 acaacttttt gcaattcttc ctttaaaatg agatccagcc aaattctagt acttcggcca
     1741 tttccttctc gaaatgggtg agcgatattc atctccacat atttcttgat aatctcttca
     1801 aaagaagttt gtggcatctg atcaatatgc tgtaacgaat gttctaaata cataactggt
     1861 gcaaatcgaa aatttccttt tgcaatattg accgatcgga tttttccggc aaaatcgtaa
     1921 atatccgaaa atagaaattc atgaatttca gataaacctt taaatgtacc aacttcgatt
     1981 tcatttattt ttccacaatc gtacaattct ttcgcttttt gtttactgag tttttcttca
     2041 accttagcca actcaacttg attggttaat cccaatttat tctctaaaat catgcgaaat
     2101 ctccttaact ttatgacata agtttatgaa acactctcaa ccccattata tcgcgatttt
     2161 gtttatgtgt caaaaactat taagtttctg acccccgata gatttttcaa gtgtctctat
     2221 tttgcacagt ctag
//
LOCUS       DARBEC010000170         2220 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.177, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000170 DARBEC010000000
VERSION     DARBEC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2220
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.177"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..>2220
                     /gene="acm"
                     /locus_tag="SCL80_002774"
     CDS             105..>2220
                     /gene="acm"
                     /locus_tag="SCL80_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEG1108599.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLL"
BASE COUNT          872 a          343 c          441 g          564 t
ORIGIN      
        1 ttagttaaaa gattggaaat gagtggggga ggaattgaag aaaggatttt tgagagatga
       61 tatagtaggt tttattttta gttaaaatag gggagataga aaaaatgaaa aagtgtagaa
      121 atattatttt atcaatgtta tttattatta ctaatataac atcactgatt cctgttcatg
      181 tctatgcgga tgcaggcaga gatatcagca gtaatgtcac ttcgttgacc gtggacccaa
      241 cgaatattac agatggagga aacataaaag taaaattctc gtttgacgaa aaaaaacaaa
      301 atatccaacc aggtgattat ctttggatca attggccaag tgaaggaaat attcgcggtg
      361 aaggattcca aaaagaaatc ccattaatga ttgaaaataa aaatgttgga actttaacgg
      421 tcagaaaaga ttctgcgcag gttgtgttta atgaaaatat taaaaaccta gactcagtag
      481 aaggttgggg gcaatttgag atacaggcca gaaacgtaac cgataccggt gaagaaaata
      541 cgggaccatt caccgtgacg agcggtgata aaacagctac tgtaaatgtg acaaagccag
      601 cttctggttc ttcttctagc gttttttatt ataaaacagg agacatgcta cctgaagata
      661 ctaagcacat tcgatggttt ttgaatatta acaataacgg cacttatgtt gaacaaccag
      721 ttaaaatcag tgatgaaatc caaagtggac aaagattaga tccaagtacg tttgaaatta
      781 atcaaataca cttaggtgaa cagaaggttt atagaggaga agagggaatc caacaatttt
      841 tacaagattt ccccagcgct acatttaatt tttcagtaac tgataattac attgaaataa
      901 ctataccaaa aaactttgtc aatcttagga aaataatggt gagttataag acgatcatag
      961 aaaacccaga acaaataaat tttgagaatc attcggaagc ttggtttaaa gaatttaata
     1021 aacctgctgt tgatggagaa agtttcaatc atacagttaa aaatatttca gcttctggtg
     1081 gagtcaacgg tacagttaga ggcgaattga agattttcaa atatatcaat gacacagaga
     1141 ttggtatccc aaatgtgact ttcgaattaa gacgagcaga tgaacagcct attcaaggac
     1201 agtctagtat cctgttgact tcgaatgaac agggagaagc tagtatcaaa ggcctgcaag
     1261 taggagacta tgttgtaaaa gaaaaagaag cacccaattg gattgatttt gatccactaa
     1321 gttcaaatga actcaaattc tccataaacg aaaatgatac agagggagtc agtttaccta
     1381 tttataacaa gaaaaaggtt acgaacatca cagcaactaa aatttggaac ggtggaacca
     1441 ctccaagacc gagtatttat tttaaattat tcagagcaag tacaaacaat tgggaaccag
     1501 ttcctgatgc agaaacaaaa cgattagaca atggaataac ttcagtgact tgggaagata
     1561 tccaacagta tgatgatagt ggaaacgaat atacgtttaa agtgcaagaa gtagatcaaa
     1621 acggaaatga ttatgtccct tcaggttatc aaaaaattga aaacgggcta gtcgttacaa
     1681 atgagaataa agaagtcatt tcagtctcag gacaaaaaac atgggaagac aatgagaacc
     1741 aagacggcaa acgaccgacg acgatcacag taaatctatt agccgacggt caaccgatcc
     1801 aacacaaaga agtaagtgaa aaagatgatt ggcgctatcg tttcacaaat ctgccaaaat
     1861 acagagacgg gcaagaaatc atctatactg taacggaaga caacgtgccc gaatacagta
     1921 cgacgattga cgggtataac atcaaaaaca gttatacacc ggaaaaaaca aatatatcta
     1981 taccaggtag ccatataaca ccgtggaaaa attttccaga gaagacagag agagagaaaa
     2041 ttagaaatct attctctcaa aaattgcaaa tatttgctca tacaggagaa tccagcgaac
     2101 aaagaagcaa ttatattagt aagagagctg tacctaaaca gataacaaat aaatcaaaaa
     2161 gcattcttcc taaaacaagt agtaaaaaat ctagttttgc tgtaatcatt ggtttattga
//
LOCUS       DARBEC010000171         2185 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.178, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000171 DARBEC010000000
VERSION     DARBEC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2185)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.178"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..723)
                     /locus_tag="SCL80_002775"
     CDS             complement(<1..723)
                     /locus_tag="SCL80_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEG1108600.1"
                     /translation="MNSWIFCIDSDGCVMDTMTYKHNLFFGPIAAEIFDVHHREKFLQ
                     DWNNINLYSETRGINRFLGLVKTLEKNDVPSINRLKHWVSVTKELSNPSLKREIEYSQ
                     SEDLLKALKWSEEVNRQISQAKGLDKPFLSARDTIKALKKYGKIIIVSSANKEAIQEE
                     WERHELLSLVDELCCQDKGKKEDIIRSVIENGCDLDKILMIGDSPGDLEAANKNKVFF
                     YPILVNKEKESWENLRTDILFKC"
     gene            complement(734..2110)
                     /locus_tag="SCL80_002776"
     CDS             complement(734..2110)
                     /locus_tag="SCL80_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1108601.1"
                     /translation="MERKFGLRDKIGYMFGDFGNDFTFIFASSFLMIFYTKVLGISGA
                     TVGTLFLVARFIDAVTDITMGRIVDGSKPSKTGKFKPWILRMSGPVALASFLMYQTSM
                     ASASMSIKVIYMYVTYILWGSIFYTSINIPYGSMASAITSVAEERTALSTFRSIGATL
                     AGMFIGVLTPIFIYTRDSSGAQIVRGGSTFTIVAGVFSVCAVICYVICYKLTVERVKS
                     DESQLKVSLLKSLKGLAKNRALLSIIAAAIFLLLAQLLTMSMNNYVFPDYYNNAQGIV
                     LMNFINPILMLVLVSPVSLIFSKKYGKKELASVGMFFSAIVYLLLFLIKPSNMYVFLV
                     LTSVGYMGLGIFNTVIWANITDVIDDQEVKSGQREDGTIYAVYSFARKIGQALAGGVG
                     GWTLSIIGYDSLAKVQSGEVLQKLYNSSTLIPAVCFFVVGLILFFVYPLSKEKVLENY
                     NLLNQKHN"
BASE COUNT          750 a          433 c          261 g          741 t
ORIGIN      
        1 acatttaaat aaaatatcag ttcttaagtt ttcccatgat tctttttctt tattaaccaa
       61 aataggataa aagaaaactt tattcttatt tgcagcttct agatcaccag gagaatcacc
      121 aatcattaat atcttatcca aatcacaacc attttcaatt acacttcgaa taatgtcttc
      181 tttttttcct ttatcttgac aacacagttc atcaactaag gataataatt catgtctttc
      241 ccattcttct tgaatagctt ccttattagc tgagcttaca ataattatct tcccatattt
      301 tttcagagct tttatagtat ctctagcact taaaaagggt ttatctaatc ctttagcttg
      361 actaatctgt cgattgactt cttccgacca ttttaaagct tttaataaat cctctgactg
      421 agagtattct atttccctct tcaaagatgg attagacaat tcttttgtta cagataccca
      481 gtgctttaat cgattaatcg atggtacatc atttttttct aatgttttaa ctaaacctaa
      541 aaatctgttt attccacgag tctctgaata taaattaata ttattccaat cctgtaggaa
      601 tttttcccta tgatgaacat caaaaatctc tgcagcaatt ggcccgaaaa agagattgtg
      661 tttataagtc attgtatcca tgacacaacc atctgaatca atacaaaata tccaactgtt
      721 catgtttttt ctcctagtta tgtttttgat ttagcaaatt ataattttct aatacttttt
      781 ctttagataa tggatatacg aaaaataaaa tcaatcctac aacaaagaaa caaactgctg
      841 gaatcaacgt tgaagagtta tacagctttt gtaaaacttc tccactttgt acttttgcta
      901 acgaatcata ccctattata gaaagagtcc aacctccgac accgccagct aaagcttgtc
      961 caatttttcg ggcaaaagaa tatactgcat aaatagttcc atcttctctt tggccagatt
     1021 tcacttcttg atcatcgatt acatcagtaa tattcgccca aatgactgta ttaaaaatac
     1081 ctaaccccat atatccaaca gaagtaagaa ctaagaatac atacatatta ctaggcttta
     1141 tcaaaaataa aagtaaatat acaatagcag agaagaacat ccctactgac gccaattctt
     1201 ttttaccgta ttttttactg aaaattaaac ttacaggtga aacaagtacc aacatcaaaa
     1261 taggattgat aaagttcatt aaaactattc cctgtgcatt attataataa tctggaaata
     1321 cataattgtt catactcatt gttaaaagct gagcaagcaa caaaaaaata gctgcagcta
     1381 ttatagaaag caacgctcta tttttagcta atccttttaa agatttcaac aatgaaactt
     1441 ttaactgcga ttcatcactt ttaacccgtt ctacagttaa tttataacat attacgtaac
     1501 aaataactgc acacactgaa aatactcctg ccacaattgt aaaagttgat ccaccacgta
     1561 caatttgagc tccagaacta tctcttgtat aaataaaaat tggtgttaac acgccaatga
     1621 acatacctgc taaggtagct cctatactac gaaatgttga taacgctgtc ctttcttctg
     1681 caacagaagt gatagctgat gccattgaac cgtaaggaat attaatagat gtataaaaaa
     1741 tacttcccca tagtatatat gttacataca tatatataac tttaatactc atagaagctg
     1801 atgccattga tgtttgatac attaaaaaac ttgctaaagc gactggacca ctcatccgta
     1861 aaatccacgg tttaaatttg ccagttttag atggttttga tccatccaca atacgcccca
     1921 ttgttatgtc tgtaactgca tcaataaatc ttgctactaa aaataatgtt cctacggtag
     1981 ctccgcttat ccccaatact tttgtataaa aaatcattaa aaaagaacta gcaaaaatga
     2041 aagtgaaatc attcccaaaa tctccaaaca tatatcctat tttgtctctt agaccaaatt
     2101 ttctttccat ataatttgcg ctccttccta ttgttaagcg ttttcattct gttgaactgc
     2161 caacttacgt actatactct gaagt
//
LOCUS       DARBEC010000172         2102 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.179, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000172 DARBEC010000000
VERSION     DARBEC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.179"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            169..1539
                     /locus_tag="SCL80_002777"
     CDS             169..1539
                     /locus_tag="SCL80_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4357 domain-containing protein"
                     /protein_id="HEG1108602.1"
                     /translation="MMDKSNEEQTTLYTKYWRRLEEVFDNSKGMEDFFRYYLAAITGE
                     YSAKHILYQAFKDYWHKERDVSSDAELLQKLVRYAGYFARLYYNKPEGKYSEVLSDFQ
                     SMESMMPAPFVLGLSEWYYHDQFITEDQYIDAIKVVNDYQLRRYFNGDDTSRVSKAFP
                     SYLKSVRKYAKANGYENIVDIVIYVLVTRNQSNNMALPSDKALKSNLLNANAYAMRLA
                     RWLLEKIENRENSATLDMSNLSIEHIMPQTSTSYWEEKAGTSGEEYTGLVNTIGNLTL
                     VTNPDNSAAGNKDFETKKKIFEDTLHIRMNKDLYELTEWTSSDISARSEALINELITM
                     YPYLRSSGDYEHDGNREIFLEAQGIKATGYLNEDETVIIHSGSEIYSKIKDIASDSLD
                     ETRQELLDNGIIEETIGGLQFVQDYTASSVSNAAALLLGGSRNGWDYWKYDNGISIND
                     SLRNKK"
     gene            1560..1844
                     /locus_tag="SCL80_002778"
     CDS             1560..1844
                     /locus_tag="SCL80_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108603.1"
                     /translation="MDKNKLRYAILKLRDEKKNPFIELKGQVPEEDILEQTKFLSRNG
                     LLESMVWADNTVHIWGSVSLEGEKYLVENSGLAKAYALAKEVKNWIPFWG"
BASE COUNT          730 a          308 c          444 g          620 t
ORIGIN      
        1 ttgatttacc aacatgttta gacattttcc tctaggaaaa aattaactag caccacgcgt
       61 attgtttata ttaaattaga tgaccgtgat aatgcacagc aaatttttga aagtatcaat
      121 tctaccggtg aaagattgac tgcctcggat ttgattcgaa acttcatcat gatggataaa
      181 tctaatgaag agcagactac gctttatact aaatattggc gtaggttgga agaagtcttt
      241 gataattcta aaggaatgga agactttttc agatactacc ttgctgcaat aactggggag
      301 tattcagcaa agcatattct ttatcaagca ttcaaagact attggcataa agagcgcgat
      361 gtatcaagtg atgccgaatt gctccaaaaa ttagtgcgat atgctggtta ttttgcgaga
      421 ctttactata acaaacctga aggaaaatac tcagaagtat tgagcgattt ccaaagtatg
      481 gaaagtatga tgcctgcacc ttttgttttg ggattatctg aatggtacta tcatgaccaa
      541 tttatcactg aagaccaata tattgatgcg attaaggtag tgaatgatta tcagcttagg
      601 agatatttta atggggatga tacaagcaga gtgtcaaaag catttccttc atatttgaaa
      661 agtgttagga agtacgctaa agctaatgga tatgaaaata tcgttgatat tgtaatttat
      721 gttcttgtta ctagaaatca atcaaataat atggcacttc catctgacaa ggcattgaaa
      781 tctaatctat tgaatgccaa tgcttatgcg atgagattgg cccgttggtt acttgaaaaa
      841 atagagaatc gagaaaattc agcaacactt gatatgagta acttgtccat tgaacacatc
      901 atgccacaga ctagtacttc atattgggaa gagaaggctg gtacatcggg ggaagaatac
      961 acaggattag taaataccat tggtaattta acactcgtta caaatcctga taattcggcc
     1021 gcgggaaata aagactttga gaccaaaaag aaaatctttg aagataccct tcatattcgt
     1081 atgaataaag atttgtacga attgactgag tggacatcat cggatatttc tgccagaagt
     1141 gaagctctaa tcaatgaact gattactatg tatccctatt tgagatcgtc gggcgactat
     1201 gaacacgatg ggaataggga aattttctta gaggcgcaag gaataaaagc aactgggtat
     1261 ttgaacgaag acgagaccgt gattatacat tcaggttcag aaatttattc taagattaaa
     1321 gatattgcat ctgattcgct agatgaaact cgccaagagc tgttggataa tggaatcatt
     1381 gaagaaacaa ttgggggact acaatttgtt caagattaca ctgcctcatc tgtttcgaat
     1441 gcagcagctc tgctattagg gggtagccgg aatggttggg actactggaa atatgataac
     1501 ggaataagta ttaatgattc gctaagaaat aagaaataga gcatagaggt ggtgttaggt
     1561 tggataagaa caaactaaga tatgcaattc ttaaattgcg tgatgaaaaa aagaatcctt
     1621 ttattgagct taaagggcaa gttccagaag aagatatttt agaacaaact aaatttcttt
     1681 cgcgcaatgg cttactagaa tccatggttt gggcggataa cactgttcat atctggggga
     1741 gtgtttctct ggaaggggaa aaatatttag ttgaaaactc aggtcttgct aaggcgtatg
     1801 cattggcaaa agaagttaaa aattggattc cattttgggg ctaagataag tttttaaacg
     1861 gagaaggtag caaatcataa ttggtttgtt gccttttttg attgcccaaa aagaaaaatg
     1921 acaaagtata gagataaatg acatagtaca cgggaaaaat gacattgtat caccgacaac
     1981 gtctaaatag atgttgatat atatacgttc cgtacaaata attataaaaa tttaaatgat
     2041 ttgagtagaa gtatcagtga atatctcaag gggaatagct atctcaagat tcgattttca
     2101 ca
//
LOCUS       DARBEC010000173         2085 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.180, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000173 DARBEC010000000
VERSION     DARBEC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2085
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.180"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            162..392
                     /locus_tag="SCL80_002779"
     CDS             162..392
                     /locus_tag="SCL80_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEG1108604.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            489..1790
                     /gene="murA"
                     /locus_tag="SCL80_002780"
     CDS             489..1790
                     /gene="murA"
                     /locus_tag="SCL80_002780"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEG1108605.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            1803..1979
                     /locus_tag="SCL80_002781"
     CDS             1803..1979
                     /locus_tag="SCL80_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEG1108606.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
BASE COUNT          664 a          345 c          438 g          638 t
ORIGIN      
        1 ttttgtctca gtcccttttt ttgcatattt ttagcattta tttcctaaaa aatgtaaggg
       61 gattcctata tttttaagta tacttgtttt tccttttata cattttatgg tattatcatt
      121 ttattagtta ttataaaaca atcgttagaa aggacgtcat tatgcaagta tacggaattg
      181 atgcggtgct tcgaatcgtg agtcatttgg cgtttatcta cttagccttc tggtctttac
      241 gttcgttacg cattgaaact tttttcagac caatgcataa tacgcaaatt agaatggcaa
      301 tcgtactttt ttcaattttt ttagggtaca ctgcaagtaa ttttttctta gaagttatcg
      361 ctttatgccg aaatcttttc gttagcctgt aaattttaaa aaaaggtact attttaaaag
      421 caaggatgtt ataatagaaa agattttgag taagtgtatg ctcattggaa ttttttggag
      481 ggaacaacat ggaagagatc atcgtaagag gtggcaatca attaaatgga acagtaagaa
      541 ttgaaggagc taaaaatgct gttttgccta ttttagctgc tagtttgtta gcagaagaag
      601 gtattacgac attagacaac gttccgattc tttctgatgt cttcactatg aatcaagtga
      661 ttcgtcactt aaacgtagat gtagattttg atgaacaaaa gaatcaagta acaatcgatg
      721 cttcaagaca attagaaatc gaagcgcctt atgagtatgt tagtcaaatg cgtgcttcaa
      781 tcgttgtgat gggtccgcta cttgccagaa atggacatgc aaaagtagct atgcctggtg
      841 gatgtgcgat tggtaaacga ccaatcgatt tgcatttaaa aggatttcaa gctttaggag
      901 caaaaattat tcaaaaaaac ggctatatcg aagcaattgc ggatgaattg atcggtaaca
      961 cgatttattt agatttccct agtgtaggtg ctactcaaaa tatcatgatg gctgccgtaa
     1021 aagcaaaagg aacaaccatc attgaaaacg tagctcgcga accagaaatc gttgatttag
     1081 caaacatttt gaataaaatg ggcgctcaag tctatggagc tggtactgaa acaatgcgta
     1141 ttgaaggcgt ggatcattta catgcagtca accactcaat cgtacaagat cgtatcgaag
     1201 caggtacctt tatggttgct gctgcaatga cacaaggcaa tgtgctgatt gcagatgcta
     1261 tctctgaaca taaccgccca ttgatttcta aattgatcga aatgggtgca gaaatcattg
     1321 aagaagaagg cggagtccgt gtcatcggac caaaacatat cttacctaca gatgtaaaaa
     1381 cgatgcctca tcctggtttc cctacagata tgcaagctca aatgacagct atccaattag
     1441 ttgcagaagg tacaagtgta gtgactgaaa cagtgtttga aaatcgtttc caacatttag
     1501 aagagatgcg ccgtatgaac gctcatgtca aaatcgacgg aaatgtagca atcatggatg
     1561 gaaaccatga attgcaaggt gcagaagtat atgcaacaga cttgcgggca gcagcagcat
     1621 tagttttagc tggcttgaaa gcaaatggaa tcacacgtgt gcgtaacttg aattatctag
     1681 accgtggata ttataatttc catatcaaac tacagcaatt aggtgcagac gttgaacgtg
     1741 tagacatgga tcaaacttct gctgaaaaaa cagcacaaac gattgcttaa ggaagtggct
     1801 gaatgagtaa ccaacgatat atcttattaa ctttgataaa gattttagtg gtgatcttat
     1861 tgcttatcct tctttttgtg gccggaacga tgatcggata tggtgtcatc ggtggcggca
     1921 atccatttaa agtattccag ccaagtttgt ggattcatat tcgagacttt tttcattgat
     1981 aaataagaat cacagtggtt ctagtcgata tgaagtgagc catgaagcag aacatgacaa
     2041 aagaaaacag aagggaacgt tgacgttagt cgcgatccct ttttt
//
LOCUS       DARBEC010000174         2077 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.181, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000174 DARBEC010000000
VERSION     DARBEC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.181"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            692..1024
                     /locus_tag="SCL80_002782"
     CDS             692..1024
                     /locus_tag="SCL80_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEG1108607.1"
                     /translation="MSYTGILSLKDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYIGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIG"
     gene            1643..1984
                     /locus_tag="SCL80_002783"
     CDS             1643..1984
                     /locus_tag="SCL80_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 domain-containing protein"
                     /protein_id="HEG1108608.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYDQTLTDNPIGFGLLPEDDEGNEWFKMTLMNDNGDELSVEDTWSYLSDYIVSF
                     EIIEFVADKEE"
BASE COUNT          729 a          279 c          433 g          636 t
ORIGIN      
        1 ataaaaatca caaacgtagc cttttacgtc ataagtgtta aaagaaataa ttaatttcaa
       61 atatagaaaa gggaacgtat gtttgcatac tccttttttt tgagttataa taatacgcga
      121 agagagataa tttcattatt taaacaagct aaaaatcttg atattacttc ttttcaggca
      181 atacgaatat tattcgctta cattttttgt ataaatttaa ttgtctgcta tgcttgtgat
      241 gaattgattt tcgttctgca cttttggatg ataaatttgg acaattgaaa atacaaaaaa
      301 aagaaaggcg gtatgaactc ggaaggctgg taaccttcgt cagcgttcat acacggcatt
      361 aagatttggc ccttaatgcg ttaacctttc agtgttacgg tattacccgc aatggattta
      421 gtataacaga tattcaaaag tgataccact ttttttggct gtgttaaggt taaaaatacg
      481 tgatattacg atgaatctat gcctaagcac aatgttataa aaatgtccgt gtggctcttt
      541 gccgtatgac gaacgctgaa attagaggga acgcatgttt ggatgcgttc cctctttttt
      601 gtttgtcatg aacgaaaatc aattctctat ttttgaaaaa taacaagaaa aacttgttaa
      661 aaatatttaa agaaattggg tggtttattt tatgtcatat acaggaattt tgagtctcaa
      721 agatatttgt cactacggta aacggtgtac cgctacagag aaaatcacga agaaactatc
      781 aactggccaa agtaaaaccg ttgtgcagtg taagaaatat ataatccaga aagacaaagt
      841 ctcagaggaa atgatttatt acattgggaa acaaaagcaa ataattttaa aagatcctat
      901 ccccttaaaa gagctatatc caacaatcaa acatgtttat gaccaaaacg gtgtactgat
      961 tggtcgaaga aaaaatggag tcttgcgttg taccgcaaaa gggatggggc gtttgatcgg
     1021 ctgaattgat cattctcata acttgtattt tatcgttttt cgcttgcttg aaaatattgt
     1081 cgagacttct aaaggaactt agagtcacta gaaaaataag gagtagactt gatctatcaa
     1141 caattccctt cggggagtga cggggtaggt tttaacgatt ggtaacgtaa aaaattggtc
     1201 aatcgagtaa atgcgcttgt ttggatagtg gtgaaatgaa tttgtgtaaa taataagcgc
     1261 agagtaaaca agagggaatt tagaagttag acaagttaga atagcgtcta gattaatcat
     1321 gagcagctac taagggatct agactttgac gcaaactcat ggaataagga gtaggtcttc
     1381 tcgtaatggg gaggacgcac aagaaattgt gtcgtgtgct gcagtacatg tagatacttg
     1441 cgaggacgtt gattattgga tcaatcgagc atttaccgct gtctactagg gtttctcgtt
     1501 tcggacgcca taaacgagtc gaaatttttt atgtattttt aagtaaacga aaaacgataa
     1561 aatatgaaca agtcctgaat aaagtgaaaa aattgatata aaacatagag aaaatgaaat
     1621 ttacgtaatg gaggaatgac aaatgaaaaa ttatgatccg aatatacgat tgggcacaca
     1681 tactattaag gtttctttcc aacgttggga ttacaaagga tttgtgacgt ttagaagagg
     1741 cggaaattgt aaaggattag atgttttagc attagacgag gatgatttat acgatcaaac
     1801 attgactgat aatccaattg gattcggctt gctacctgaa gatgatgaag gtaatgaatg
     1861 gtttaaaatg acgcttatga atgataatgg agacgaatta tctgttgaag atacttggtc
     1921 atatttgagt gattatattg tcagctttga aatcattgaa tttgtagcgg ataaagaaga
     1981 ataaaaactg gaggaataaa ttttacagat aaaggcttag aaggagtcac gatgattaat
     2041 aaaaaggtag tattacctta ggcattgagt aaggaat
//
LOCUS       DARBEC010000175         2065 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.182, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000175 DARBEC010000000
VERSION     DARBEC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.182"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1008
                     /locus_tag="SCL80_002784"
     CDS             <1..1008
                     /locus_tag="SCL80_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108609.1"
                     /translation="LLDAPYYLSKVALGENLIKPESQQPVTNSNYLINTYNIKPMVKG
                     KKYTITLEGTKPATQVFRPFFTRATGDAWEVGDLQPVEGLTDVWSGTFTAAADPHPTS
                     PQVQICQVPSTSLGNCTIKWLKLEEGDTRTPNIEQYKYRGIGMRDSNNPKDYVWDLAP
                     EYVEDNLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANAT
                     AIDELDNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGRSSGGNDST
                     ILTIPEGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYT
                     I"
     gene            1025..1174
                     /locus_tag="SCL80_002785"
     CDS             1025..1174
                     /locus_tag="SCL80_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108610.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1171..1617
                     /locus_tag="SCL80_002786"
     CDS             1171..1617
                     /locus_tag="SCL80_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108611.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1619..1756
                     /locus_tag="SCL80_002787"
     CDS             1619..1756
                     /locus_tag="SCL80_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEG1108612.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1794..>2065
                     /locus_tag="SCL80_002788"
     CDS             1794..>2065
                     /locus_tag="SCL80_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108613.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLVEHTQQIKT"
BASE COUNT          753 a          358 c          425 g          529 t
ORIGIN      
        1 ctacttgatg caccgtatta tttgagtaag gtggctttgg gtgagaatct aattaaacca
       61 gaatcacaac aaccagttac taatagtaac tatcttatta acacctataa cattaaacca
      121 atggtaaaag gtaagaagta taccatcaca cttgaaggaa ctaagccagc aacacaggtt
      181 tttagaccat ttttcacacg agcaacagga gatgcatggg aggttggtga cttacaacca
      241 gtagaagggt taactgatgt ttggtctgga acatttacag cagcagctga cccacaccct
      301 accagcccac aagttcagat ttgtcaggta ccaagcacaa gtttaggaaa ctgtacaatt
      361 aaatggttaa aactagagga aggcgacaca cgaaccccga atattgagca atataaatac
      421 cgaggaatcg gcatgcgaga ctcaaacaat ccaaaagatt acgtctggga tctagcacca
      481 gaatatgtcg aagacaatct ggccacagat attaaaattt ctgaaattac tggtaaagca
      541 aacaattata ccgatgggaa agtatcggag attaattcgc agttgactgc ttcaattaat
      601 gaagtagaca ccacagctaa ggatgctcaa acaaaagcga atgctaatgc gactgctata
      661 gatgaattag acaataagat cgatgaacgc attaatgata cagctactac cacattaaca
      721 gttacaaacg ggaataccgg atcagcaaag ctttatcgtg aaggaaaaac agtttctata
      781 tattttgtgg ctttaaacgg aagaagcagt ggtggaaatg attcaacgat actaacaatt
      841 ccagaaggct atcggccacc aattagtttt gagcaactgg ttggctcgat agaccgttct
      901 actttgaaca gtgctcagtt atctattggt gcagatggag ccattaaatg gcgaagaaac
      961 tcaagttatg gatcggatta tacctttgca attacttaca cgatttagaa aagcgtgaat
     1021 cgatatgaag gcagcatata gaccaattga accttacgga ttcgagcaaa tcattgtgaa
     1081 tgatgaagaa catttaccgg aagaatgcac agaagtcgaa ccaccgattc caaattggaa
     1141 accgaaattc aattactggg agggaaataa atgaaaaaca tttggaaata tggacgtact
     1201 ggcggagagt acgcaggaaa agtattggac gacatgcttg tatccgttcc ttacacagat
     1261 cagccaccgc ttgaaggaat tcgtgctgat ggcgaaccgt taacaatcgc tgatcaaatg
     1321 tttgatccta aattgaatca atggattatt ttagcgaacg cactagatca caacgattta
     1381 aacaatctca aagcaatgta tgagtcgtta gaaaatgaga acggcgattt aaaacagatc
     1441 aatgccaaac tcatgctaag cgatgtagca attaaacagg aaaatactgc attgaaagaa
     1501 aaagcggata gtttagcaca aatcaattca aaaatgatgc ttgcttcgtt acaaaatagc
     1561 aaagacattt cagaaattaa agagcaacta aatccagctt caaagggagg tgagtagtat
     1621 gtttagtttt agcgatgtga aaatgatgta tgattggggc tgtttcacta acgaacaagt
     1681 aatggttttc gttccgttgt gcattactga agaaaaagca gataaaatca ttagcaaaga
     1741 agagagcgca tcttaattga tgtgcttttt attttgattc aaggagttgt cacatgatta
     1801 atttagggga atggggagcg atagcaggat caataaccgc tatcgtttct ttgattttat
     1861 tagtaataaa accaattact gcatctttct cgaagattac tgagactctt tcaaaagtaa
     1921 gtcacaattt agatttgctg actaaagatt tagaatcgag caaatcagat cgattgatga
     1981 ttcatgaaga actaaagaaa cacgatgaaa gattagatac acatgcagaa aaattggtag
     2041 aacacacgca acaaattaaa acttt
//
LOCUS       DARBEC010000176         2040 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.183, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000176 DARBEC010000000
VERSION     DARBEC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2040)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2040)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2040
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.183"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1872
                     /locus_tag="SCL80_002789"
     CDS             <1..1872
                     /locus_tag="SCL80_002789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1108614.1"
                     /translation="SAENMEEIKKMFPKSTWFGFTGTPIFPENRKQAKGQLARTTHDQ
                     YGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENKIYRKLKEVETYAEYSSEQIN
                     RMIDQMEPVKKESYLDPAVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQLIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLAIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPDKTKGLIVTFRKPATMEKN
                     VEDATKLYSKAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSPTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYL
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          738 a          330 c          416 g          556 t
ORIGIN      
        1 agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg gtttggcttt
       61 acaggtacac cgatttttcc tgagaatcgg aaacaagcca aagggcaatt ggcacgtaca
      121 acccatgatc agtatggaga ggtcttgcat acctatacca ttaagaatgc gctagaagat
      181 ggctctgtct taggatttca agtagagcat gaaaacaccg tggagccgac ttcactagag
      241 aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc atcagaacag
      301 atcaatcgta tgattgatca aatggaacct gtcaaaaaag aaagttattt ggacccagct
      361 gtttttgaag cagatgaaca tatccaaaaa gtcattcata aaatgttccg tccagataat
      421 gcctatacaa aatttgattt tcgaaatggc aggccaacga aatctgcaat cttaaccacg
      481 agttccattg acatggccaa aaagtattat cgggccatca aagagatgac gaaagagcct
      541 gattggttaa caaaagagtt tcccaatcag ctcattcgtg aaggacgaac gatggaggat
      601 cctgattttc cacgaatcgc tattacctat tcattagaag aaaatagccg agattcaagt
      661 gcccaacaag aagaaatgca aaaaattatt gaggaatata atcagtatta tgatacttcc
      721 tggtcattag cagacattga gcgttataac ggagacatca ataatcgttt ggcacgaaaa
      781 agagcggaat tcaaacaatt tgggaaacaa atcgatttag cgattgtagt ggatcgtcta
      841 ttaacaggtt ttgacgcccc tacgattcaa accctatttg tggatcgtaa tctagagtat
      901 gcaggattga tccaagcttt ttcacgtacg aatcgtacgt atcctgataa gacaaaagga
      961 ttgattgtga cgtttcgtaa gccagcgaca atggagaaga atgtcgagga tgcgacaaaa
     1021 ctgtattcaa aagcaaaaga agaatcaggt ttgatttatc caagttatca ggaatccaaa
     1081 aaaagattca aacaagcata tggtaagtta aaagaggttt catccatgga atccattgat
     1141 gaacatacgc cacttgaaat ccgtatcgag tacgtcaaag catttcaaga attgaatcgt
     1201 gcttatgaat cgttagtgac ttatgacgat tacaatgatg acatggtgaa atctccaact
     1261 ttgaacaatc aaattcaact tttggaagaa caaattggcg tgtacgagac cgtgaagggt
     1321 tcattaatag aggaagaacc agaaaaagaa ggagaagatt tttcaacgat cgaattttat
     1381 agtgaaaata ccactaagtt gtatgatatt gattcaactt atattgatca attactagga
     1441 acttatgctg cgaatagttc agatgcacga gaagaaattg aaaaagcttt ggctaaattg
     1501 aataaaacag aaggagtcaa agaagtttac cgacaaattt taaatgcatt tgatgtgggg
     1561 actttagata gtaatgaaga tatctttgtc attaaacgtc gctattttac acaagcgagt
     1621 gatgatttga ttcatgtctt ttccaatgag tggtttgttt ctgagaaaga attacatgct
     1681 tctaatctgc aatatctacc aggtgaagat ccaattccta atatgaaagc aattatcaac
     1741 agtaaggatt acgaaggata taaagccaaa catccagaag cgaagccatt taaataccca
     1801 caagaaatga agcgagcatg gcgtaaaatg ttagatgatg agttgattcc acttgagaat
     1861 gaattgaggt aaacgagaag agttaagaaa attgtacgat tcaaagatgg tagtcaagtg
     1921 gaagtgtaga aaatagggat agatgtcttt tatttgttca cattttataa cgaagtattt
     1981 aagacagcta ttgaataatg tttcctccac ctttttcatt catttaccgg ttctgttgca
//
LOCUS       DARBEC010000177         2003 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.184, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000177 DARBEC010000000
VERSION     DARBEC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.184"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..378
                     /locus_tag="SCL80_002790"
     CDS             <1..378
                     /locus_tag="SCL80_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEG1108615.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="SCL80_002791"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="SCL80_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEG1108616.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="SCL80_002792"
     CDS             1859..>2003
                     /locus_tag="SCL80_002792"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108617.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DARBEC010000178         1986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.185, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000178 DARBEC010000000
VERSION     DARBEC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.185"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..425)
                     /locus_tag="SCL80_002793"
     CDS             complement(<1..425)
                     /locus_tag="SCL80_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEG1108618.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIP"
     gene            complement(534..1565)
                     /locus_tag="SCL80_002794"
     CDS             complement(534..1565)
                     /locus_tag="SCL80_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1108619.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
BASE COUNT          611 a          327 c          343 g          705 t
ORIGIN      
        1 ggaatctgtt ttgattttgt tccatttgaa atttctctca ctcgcttttt tgtctcatca
       61 tcaaataaaa tgaaagggtc ttctaaacga tcgataaata cttccattgt tgttgttggt
      121 agatgataag aagtcatgac aggcactgca ccaaggtaag acacagaaac agctagaagg
      181 tatgtgtcaa aagcggaact cttataaata ataactttgt ctccggattt cactccaaga
      241 tgagccaatt gataagcccg tttcaacaca ctttcgcagc attttttgta agttgtttca
      301 agtcctaatt ctgggaaagc tgaaagtggc tcatcaaaga taatcggggt ctcggaaaag
      361 ttagctgcag cttcttgaaa attcgtaaat aagttcaaag gggaatacgt tatggtctcc
      421 atcattcaca ctccattatc ttttgttaag taatatacaa attcaagata atcgtaacat
      481 aatcctttat ataataacat agtttattgt gaaaaaaaga tcatacattt gttttatatg
      541 gaagaaaccc gaacgaatgc cgctaattcg tgataagaaa aatcaaccat cttttcccag
      601 atatcttgaa aagaatggga tgagaggtat cgattaccac ataaataatt taccgcgtca
      661 gcatcatcta aatatagttc tgcataataa cgatatgttt ttctgcatag ttctggcgat
      721 acgtttttat atactgtaat tttgctcaaa atttggcgaa tagtccgagg agaaatttgc
      781 tgatcatgtg aggaacgaaa aacaaaagaa ttttcgtccg tattttgtgc tgttaactta
      841 aaatagttct ccagaaatgg catcgcttct tttggtattg gtactttatt cagtctgttt
      901 tcttttctat tcagattaag aatctgcttg gtcaaagata catctttacg tttaagttct
      961 actacttcat ggatctttaa tccgcaatac agaagaatgg cagtaattgc aaggttcctc
     1021 ggtctattct tttcttcaaa gctagcttct ctgcttgttt gaaagccttc aatcgggata
     1081 aacaaatgct caaaatcttt gaaaagttct gcatcgatat atcgcggagc tgctttggtt
     1141 gtttccggta aacttgtacc caaaagagaa atctcttctt caatcagtcc tttttgatac
     1201 aaaaatcgta aaaaatgatt taatgaatat tgcttccttg aaatcccgta atagctattt
     1261 tcaaacaatt gctccgtttc ttggttcgct gtagtttgga acgaacgagt atgtcgatag
     1321 attttagcta agaaattctg ataatcttgt ttttctaaat ggataatcag ctgatcatcc
     1381 acttcttcag aaaatacttt tctcaaataa tcagtaaaca gatgaaaatc ttttgcgtat
     1441 tcatagcgac tgcctaatga aagggaagaa cgattttctt ctaaaaactg ttgaaagacg
     1501 gaagggagca agtgtgtacg ttgctttaac ttttcttgca gttgttttct agttttgctg
     1561 tccattacaa attccctcca atagtgttca ttttctatat tttaccgcat ttcatcaaat
     1621 gaagatagac agtttgtctt gatcttagga agcaaatttt taactaagta ctagctaaaa
     1681 gatatgaagt aagaagatac atttttagtt attttattag tgcagtcatt cttttgatct
     1741 tttaattgat ttatcctctt tttagctatg tggttacttc atgagcaatg aaatggattt
     1801 ggttcgttct aatggcagga ggctctttct tttatttccg aaaaattaga aaacattttt
     1861 tcagaatcga ttggctctcc atttattcaa taatggtttg tctaacgata ttgttgggat
     1921 aattcttaaa gaagtattag aaatagttgg tacttcatta ctttacatga agatttattc
     1981 tatggt
//
LOCUS       DARBEC010000179         1977 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.186, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000179 DARBEC010000000
VERSION     DARBEC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1977)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1977)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1977
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.186"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..427)
                     /locus_tag="SCL80_002795"
     CDS             complement(<1..427)
                     /locus_tag="SCL80_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEG1108620.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLK"
     gene            complement(424..1077)
                     /locus_tag="SCL80_002796"
     CDS             complement(424..1077)
                     /locus_tag="SCL80_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEG1108621.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            1365..>1977
                     /locus_tag="SCL80_002797"
     CDS             1365..>1977
                     /locus_tag="SCL80_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108622.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGRLDDSP
                     SHCEHCGSIRIIRNGSYTTRTQILKVKEKLTILELKRTRFLCYDCGQTFSAKTDLVDE
                     HHQLTKELKQAILIELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSMKSVSGSMSFICVDGTTHQLFTILEDRRLYKLTQYFL"
BASE COUNT          622 a          351 c          254 g          750 t
ORIGIN      
        1 atttaaggct taaatctttc gcatcttttt gcatatcagc tgtagaaaca aattgtagat
       61 ttgttttttt accatcatta agctttattt ctctttcttc aattatcaaa gaattactat
      121 cactttctaa attaacattt atattatctt tgatttgtat ttcattctta ttattttttt
      181 cttttatgaa tacttttatt tcactactct tggaataaaa ctccaaagtt tgttctatat
      241 attttatttc ttttccattc atattactag aaatttcatt tgccatatta tgaatctttt
      301 cttttctcgt ctccaaatat gttttaggaa agataaaaaa aattaatgaa tgaactaata
      361 taattattat tccaagaaca gaaaatattt gtatgaacat ttttggaaat atctttaatt
      421 ttttcatttt ctctccaatt tatatcctac atttcttata gtagttatac agtctaattg
      481 taattttttt ctaagttctt ttatatatac atctattact cgatcataag gagtttcttc
      541 gctatctttc catacataat caataatttg cattcttgtt aacacttgtc cattattatc
      601 caataaacat tttaatactt ctagttcttt tgcatttaca tctatttctt caccatttat
      661 tttagctgta tagctattaa aattaaccga tagatcctta tattcaaact tctctaaatg
      721 tccataattc ttcttaatta aagaatctat tctagctttt aagaccggca atgaaaatgg
      781 cttttctaca tatccgtcta ctaaattagt aaatgcatca attttatatt cttcatcact
      841 aaatgcagtc aagattagaa ttggtaaatt gcttttcttt ctaatttctt tcaacacttc
      901 taaaccattt ataaaaggta tctgaatgtc taagataacc aaatttatat cgctattaaa
      961 ttttgacaag gcttctcttc catctttagc ttgaataaca gtatatccaa attctgaaag
     1021 atattcactt attccctctc ttatcatatt atcatcttca acagttaata ttttcatata
     1081 tacctccact taagcctata cactagatat tatacagcat attatggctc tataatatct
     1141 agtgttggtg acaccttctg ttgccaatac tttttctttt ttctaataaa tgcaaacaga
     1201 caccttgaaa tcctttagaa taaagggttc tataatattt tgtgttgttg gaaaatcgaa
     1261 ggcgattttc tactaacact ttttcttcgt ttcaactcaa aaagagactc acttttggta
     1321 aaatttagtc gaccaaaaca tcaccctacc aaaggagtcg ctctatggat aatcatacta
     1381 gaaaattact cggattaaca gataaacatt tattttttga tgaagaatgg ctgattgaaa
     1441 aagaatataa aggcgttcaa gcgcagttta ttaaagggag attggatgac tcaccttccc
     1501 attgtgaaca ctgtggatcg attcgtatta tccgcaacgg ttcctatacg acacgtactc
     1561 agatattgaa agtcaaagaa aagttgacta ttttagaatt aaaacgtacc cgttttctat
     1621 gctatgattg tgggcagacc ttttctgcga aaacggactt agttgatgaa catcatcaac
     1681 taacaaagga actgaagcag gcgattctca tagaactgta cgaaaatcaa tcacgtaaac
     1741 taatcgctaa gaagtacttc gtctcggatg ggacggtgac acggatcttg cgtgaagcaa
     1801 cgaagcatta ccagccacgt atgaacttcc ttccgacggt gttgtgtatg gatgagttca
     1861 aatcaatgaa gtctgtttct ggttccatga gttttatttg tgttgatggg acaacccatc
     1921 agctatttac gatccttgaa gatcgcagac tttacaaact aactcaatac ttcctgc
//
LOCUS       DARBEC010000180         1970 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.187, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000180 DARBEC010000000
VERSION     DARBEC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1970)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1970)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1970
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.187"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            274..840
                     /gene="amaP"
                     /locus_tag="SCL80_002798"
     CDS             274..840
                     /gene="amaP"
                     /locus_tag="SCL80_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEG1108623.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
     gene            853..1041
                     /locus_tag="SCL80_002799"
     CDS             853..1041
                     /locus_tag="SCL80_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEG1108624.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            1054..1611
                     /locus_tag="SCL80_002800"
     CDS             1054..1611
                     /locus_tag="SCL80_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEG1108625.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            1643..1885
                     /locus_tag="SCL80_002801"
     CDS             1643..1885
                     /locus_tag="SCL80_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEG1108626.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
BASE COUNT          719 a          305 c          404 g          542 t
ORIGIN      
        1 aaataaatag aatcttgtaa taataactga gcataatctt tgcaattttt aatcttgaat
       61 aatatccttt aagtatagcc agcaatcaaa agtaaataga gaataagtaa gcaattgtta
      121 tttcagtatc agactggcaa taaaacgccg ttatgatcca gcaatgtttg gtagcgcttt
      181 atttactgat gctatgctaa ctgttcgcta tctatactgt gattgtcggt agagttattt
      241 acagggtaaa aataagttta taggaggtgg aaaatgaata aggggttaaa aataatcggt
      301 gttatcatga gtttactgct actttcagta tttctagcga ttgcgttgat caatgcgccg
      361 tatattatgc ctgaacaatt ggaacgattt cgcttcttta ctttaacgaa ttactacatg
      421 caacaatata ttttctgggc agctgtagta tttgccgttc ttgctattat catcttgcta
      481 gtcgttcttt tctatccaaa gtcaagagga actttcgtta tgaaacggga agatggcaaa
      541 ctaacaatcg acaaaaaagc gatcgaaggg ttggtacgtt cccatttaca cgaagaagag
      601 ttcattcatt cacctaaagt acggatacgt tcaacgaaaa atcgaatcca tatccatgtc
      661 aacggagact tgaaacgaac ttcttcatta gtagggaaaa caggtgtatt gatgcaagat
      721 atcgaagaag aagtaactaa agtactcgga acaaaagaaa cggtcaaagt ggcagtcaca
      781 tacagcggtt atgaagaaca agaagataca agagactaca agcactctcg agtggaatag
      841 gagggccaaa taatgaaaga actattttca gcatacaagc tccctattat ctttgggggt
      901 cttggactac tgctagctgt tttatggtta acgattggat ttttcaagac attgttgatc
      961 ataatcatga cagcaattgg cgcaggaatc ggtttttatt tgaaacagtc aggaatagtt
     1021 gatcaatttt ttaaatcata ggaggaaaca aagatggaaa acaaaaacat gaatacaaca
     1081 aatggatcag ttttggcaaa tgaaaaagga gaaaaccgca tgatgaatgg tgaattgact
     1141 tatgaagaca aagtcatcca aaaaatcatc ggttatgcat tgaaagatgt agatggtcta
     1201 ttgacattag atggcggatt tttctcaaat atggctgaaa aattagtcaa cacagataac
     1261 gtaacagcag gtatcaatac agaagtaggt aagaaacaag tagctgtgga tctagatgtg
     1321 atcgtagaat acggtaaaga catcgaagat atttacaacc aaatcaaaga attgatcagt
     1381 actgaagtaa acaatatgac acatttagat gttatcgaag tgaatgtgaa tgttgcagac
     1441 atcaaaacac aagaagaata ccaaaaagat caagaaacag tacaagacaa agtaacagaa
     1501 gcagcaaaat ccacaggaca atttgcttca cgccaaacag ataaagcaaa atctgctgta
     1561 ggcaaaggtg cacaaaaagt caaagaaaat aacgaaccac gtgtacaata agaagtaaga
     1621 agtcagttag gaggaattta aaatgggatt tatttgggca ttaatcgtcg gcggtatcat
     1681 tggagcaatt gcaggagcaa tcacgaataa aggatcatca atggggatca tcgcaaacat
     1741 cattgctggt ttagtaggtt cagcaattgg acaagcttta ctaggaactt ggggaccaac
     1801 aattgcagga atggctatca ttccatcatt gatcggtgca atcattttag tattagtcgt
     1861 ttcattcttt gcacgtaaat tataagcaaa ttgaagaaaa acttatgttg aaatgaagaa
     1921 caaatttgag aaccgtagcg acactcgctg cggttctttt ttatatattt
//
LOCUS       DARBEC010000181         1945 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.188, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000181 DARBEC010000000
VERSION     DARBEC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1945)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1945)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1945
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.188"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..695
                     /locus_tag="SCL80_002802"
     CDS             <1..695
                     /locus_tag="SCL80_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108627.1"
                     /translation="YLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYFVYCPELLLYD
                     VKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYGIDETDIFAKV
                     NSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTLLISPISTISF
                     SPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLSEEKILLPENW
                     AELRKLVEYGII"
     gene            682..>1945
                     /locus_tag="SCL80_002803"
     CDS             682..>1945
                     /locus_tag="SCL80_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1108628.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKI"
BASE COUNT          665 a          246 c          298 g          736 t
ORIGIN      
        1 cttatttgaa gaagagtata ttttcaacaa gtaaaaacgt tcaaagagtt tttaaaatat
       61 taaataaaaa tagcatcttc aaaaaaaaaa ttgacgatta ttttgtctat tgtccggaat
      121 tgttacttta tgatgtaaaa aatataaaaa aaattcccaa aatagatgaa aacagtaaat
      181 tgatgaaaaa catacaatca atctttggga tagaagaagt ttttgtcgga gataaatgta
      241 tcttttttga tcaaccttta ttatctgatg gttatggcat agatgaaaca gatattttcg
      301 ctaaagtaaa tagtatattt tctggaaaaa aagaaacttt aattaagctt catcctagag
      361 caaggacaaa tcgttatggt gaaaaaaaca tattgaattc cagtttaccg tttgaagtct
      421 tgctacttga aaatgacttc tcttcaacac ttttgattag tcctatatct acaatttctt
      481 tttcaccatc tatgatgttt gaaaagacga tcaactctat tatgttggct aaattaattt
      541 taaatgaata tacagcatta tctgaagata aaaaagaaac attgacaaat attgttcttt
      601 tctgtgaaca ttataaccgt ctatcagaag aaaaaatttt actcccagaa aactgggcag
      661 aattaagaaa gttggtagag tatgggatta tttaacacac tttccaagtc aaaattagtt
      721 aagtccagtt tctggtatac tatagggaat ttttttatta agggaatttc attcataact
      781 attcctattt ttgtaaatat tactactcaa gcagaatatg gtttgattaa taatttcact
      841 tcatttgtat caattttttc gattcttgtt gggctaagcc taaattcggc aattaataat
      901 gcaagttttg actataaaga tgacataaat gaatttatgt catcggtact ttctctagct
      961 acactttcgt taattgtgtt cttattaata gggaacatat attttttgtt ttcaaatgct
     1021 ttttttgata tgaaccaact tgtatttaat ttgatgttat tacaaagttt tgctagtttt
     1081 gtaattagtt atatccagtc ttattttacg attaacttta accattttaa atatttgatt
     1141 ttatctttta tatctacttt tttgaatatt gcaatctctg tagctttgat gcagacatta
     1201 tattttgatg ataaatatgt tggcagagta gtcggcggaa cactgtcatt tgtgttgata
     1261 ggtttagtaa tttatttaag aataatgata aagggtgctc atttttttga taaaaaacag
     1321 tggagatacg ctttaaaaat ctctttgccg ctaatcccac attccttagc aaatatagtt
     1381 ttagcacaat tcgatagaat tatgattaat tcttatttag gtcctgctgc ggggggaata
     1441 tattcctaca ttagtaacat aggtattatt ctaagcgtaa tatggatgtc tactaataac
     1501 gcctgggttc catggttcta tggtgagatg agtaaaaata atgaaaaaaa agtgaagaaa
     1561 gttagtaact attatttagt agtctttact ttgattacga tgattgtaat ggtagtttcg
     1621 attgatttag caaaatttat ggctccgaaa gactattatt ctgggttatc tttagtggtt
     1681 ccgataacct taggctattt ctttcagttt ttatatagtt taccagttaa tgctgaattt
     1741 tatgagaaaa aaacatcgtt tattgcgatt ggtacagtag caagtgctat agttaatata
     1801 ctattaaaca tgattttcat acctaggtta ggaataattg ctgctggata cacaaccgta
     1861 tttacgtatt ttttactttt tatttttcat tataatatcg ctaaaaaaat tacgaataaa
     1921 caattatttg acacaaaaaa aatat
//
LOCUS       DARBEC010000182         1942 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.189, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000182 DARBEC010000000
VERSION     DARBEC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.189"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..221)
                     /locus_tag="SCL80_002804"
     CDS             complement(<1..221)
                     /locus_tag="SCL80_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1108629.1"
                     /translation="MTSRKTKDERTRNWTFVVYPESAPENWREFLDELHVPWVESPLH
                     DKDVNPDGELKKPHWHVLIMFSGKKTYDQ"
     gene            complement(288..443)
                     /locus_tag="SCL80_002805"
     CDS             complement(288..443)
                     /locus_tag="SCL80_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEG1108630.1"
                     /translation="MISNEKKRVVVTLTKEVADNLDKIAKQMGLSKSGLITIWVNANG
                     KENEQKK"
     gene            1060..1281
                     /locus_tag="SCL80_002806"
     CDS             1060..1281
                     /locus_tag="SCL80_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108631.1"
                     /translation="MRVIKSEKFTLAEIMTTSELAQELDYNQAYVLRLAKEHLTEGKE
                     YRSAGRRNYLFSPEALKKLEKVITTKEGE"
     gene            1282..1539
                     /locus_tag="SCL80_002807"
     CDS             1282..1539
                     /locus_tag="SCL80_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108632.1"
                     /translation="MPKETTINVEQLEKLLESYSNDFIELTGGKVEETTDLFDLLLTN
                     ILELNSEEQKEHKGKLIAPSYRKVAKSAVPTGFLTKQPYIF"
     gene            complement(1675..>1942)
                     /locus_tag="SCL80_002808"
     CDS             complement(1675..>1942)
                     /locus_tag="SCL80_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEG1108633.1"
                     /translation="SDIIAHGGAEIASYLSVTSAERYELIREMMSFVDSKNITEIKDL
                     IDYAMSERFDDWFPLLCDNSAYIIGQYIKSNRHGGSVNSKINKG"
BASE COUNT          648 a          441 c          293 g          560 t
ORIGIN      
        1 atttgatcat acgtcttttt accgctaaac attatcaaaa catgccaatg aggctttttc
       61 aattcaccgt caggattcac atctttatca tgaagtggac tctcaaccca aggaacatgt
      121 aactcatcta aaaactccct ccaattttca ggagccgatt caggatagac tacaaacgtc
      181 caattccttg tgcgttcatc tttagttttt ctactcgtca tgctataatt accttaattg
      241 aacaacagtc taacagcaaa agcagtgcgc caacactaat gcttttgcta ttttttttgc
      301 tcattttcct ttccgttagc attaacccaa attgtaataa gaccagactt actaagaccc
      361 atttgtttcg caattttatc aagattatct gccacttcct tagtaagcgt tacaacgact
      421 ctcttttttt cattactgat cataattcgt atcctccaac gactttttct taaaaaccat
      481 agtaacaaaa aagtaactaa ggtgcaacag tttagatact aattagcgaa taataggcct
      541 taaacccttg gcattactga ttttgtcaaa aaaaaacgct gaaacccttg ggagagtaag
      601 gctaaacagc tatcgaccaa tgaccaatga ccaataaggg gggtgtcgta gtccccccct
      661 cctccgccca attcataaac accaaaacca aaaaacaaac ctcagggctc cgccccgaac
      721 cccgacctcc ttctaccgaa atatatgcag gcgccacgct tactatttcg ttcagctatc
      781 ggcttttgca atctaacacg accgtttcgc taccagtcgc cttacaggct ccattctcgc
      841 ttctcttacg tgttttaacg attgcgccga taactaaacg aaatagacgc taaatcgctt
      901 aaaacgcaaa atagaccctt tttagagcat tgattttttc tactagtcat ttcgataata
      961 taaaattgac cacaacacag aaaacttata ttataagctt atgtcgtaag tccgacgtac
     1021 tcacgtaata tagacggacg ttaaatggaa ggagcataaa tgagagtgat aaaatcagaa
     1081 aaattcactt tggccgaaat tatgacgacc agcgaacttg cacaagaact tgattacaac
     1141 caagcttacg ttttgcgctt agcgaaagaa catctgactg aaggaaaaga atatcgctct
     1201 gccggtagaa gaaactatct tttcagccca gaagccctta aaaaactaga aaaagtcata
     1261 accaccaaag aaggtgagta aatgcctaaa gaaacaacta tcaacgttga acaactagaa
     1321 aaattgctag aatcatattc caatgatttt atcgaattga ccggcggaaa agtcgaagaa
     1381 accaccgacc tctttgacct acttctcaca aatattctgg aattaaactc tgaagaacaa
     1441 aaagagcata agggaaaact gatagcacct tcctacagga aggtggctaa gtcggctgtg
     1501 ccaactggtt ttcttacaaa acaaccttat attttttgat atcacaaaaa aatccatttt
     1561 ggacctattt tcggtcataa tatagtacct acttttggtc atagtttcgt ccgtagtttt
     1621 ggtcatagtg actaagactt tcacgcacaa aaaaagagct gaaattaaca gctcttatcc
     1681 tttatttatt tttgaattta cacttccacc atgacgattc gattttatat attgcccgat
     1741 aatataagct gaattatcac aaagaagcgg aaaccaatca tcaaatcttt ctgacattgc
     1801 atagtcaatt aaatctttga tttcagtgat attcttagaa tcaacaaacg acatcatctc
     1861 tctaatcaac tcatatcgtt ctgcactcgt tactgacaaa taactagcaa tttctgcacc
     1921 accatgagcg ataatatcac tt
//
LOCUS       DARBEC010000183         1890 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.190, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000183 DARBEC010000000
VERSION     DARBEC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.190"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..280)
                     /locus_tag="SCL80_002809"
                     /pseudo
     CDS             complement(62..280)
                     /locus_tag="SCL80_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(546..740)
                     /locus_tag="SCL80_002810"
     CDS             complement(546..740)
                     /locus_tag="SCL80_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108634.1"
                     /translation="MMGFFLRFYIIELTGRLTVFFGNFAIKPFAEVTAHYICKWLDCK
                     NKIKKVFHYISSSPCQIRPG"
     gene            complement(840..926)
                     /locus_tag="SCL80_002811"
                     /pseudo
     CDS             complement(840..926)
                     /locus_tag="SCL80_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT          657 a          266 c          251 g          716 t
ORIGIN      
        1 taggtatttt tattatatat agatttaaca tatgtcggtc ggttgattga tatagctaaa
       61 gttataactc aaccgaagcc gattcatcta cacgaataaa gttgcgagtg ttctcggcta
      121 attcataaaa attgttatgt gttccatata gcaatactgg aataacttta ttttcttgaa
      181 tctcataagc aactctatac acggtgaccg catacttaaa tccattagac caattacctg
      241 ataaacctcc agttttttgc tcttacttaa atggatcaat ggcaatttca tcacaaacct
      301 atagaggatg agtgacttct gcctaattac gttaaaaaat attgaaagta actctatgta
      361 gcaaaaaata tctagaattt catttcctca aaactgatcg atattgtatc taaaaatata
      421 tactacctaa aataggtagt atatattaaa atatgtgctt ttgttagaaa aatcaataca
      481 taaattatca tatctgtata tgtcatttag gtatttcagt tatgttataa tagttttgct
      541 gtctgctacc ccggcctaat ctggcaaggg gaggaggaga tataatgaaa tacctttttg
      601 attttgtttt tgcaatcaag ccacttgcag atgtagtgtg cagttacctc tgcaaatggc
      661 ttgattgcaa aattgccaaa aaagacagta agcctacccg ttaattctat aatatagaaa
      721 cgtaaaaaaa accccatcag cctgcacgct gatggggttt ggtgatataa tgaaatacct
      781 ctttgttact cataattgta ccataattag tcacgaatgc aaccaataga aaaaactact
      841 tagaacggta gatcatcgct aataacaatg cattgtccac caaatggatt ttgtgaagca
      901 ttaaatacat tgttattaat tgtcgctgct tttaactagg cctctcccct tctattataa
      961 aagaagatat atatcatttc ttaattacct actaccttaa accaaaattt aagtactacc
     1021 taatttaagt attacttaaa caatctaaaa aatagttatc ccaattgtta tgcttttata
     1081 atacgattat ttgtaacaaa atgaatatta tataattaaa cactaccata tttaggtagt
     1141 gtttaattat tagcaaaaca aaaaatattt attctattct cttaacttca actaagttat
     1201 gctgcacacc tttatataaa tcatatgcaa ctcattagct agacaaacta caagcatgta
     1261 gttttatctg ttaattatgt actacctaaa taaagtagtt tataattagc aagtatatat
     1321 atttaaaaat ttaagattaa tttttttatg acttgtacaa atttatctga tattctctta
     1381 ttaagaacta cctaatttgg gtagttctta ttttttacat tttaagtagt atttaattgc
     1441 aatttggtag tattttttta ttatcttatt cttagaacgg tagtacttaa tataaatata
     1501 ggtagtatct aatttaagta ttaatggtag tatctaaatt aacaggtagt atgtaactac
     1561 tttaaagtaa tttttttcag attaacacag tagtatctta ttaaataagt taattttaat
     1621 ggtagtacat aattattata tatatctgac attggtagta cttattgtat tttttagtag
     1681 tatataatct attgttttct agcctaaagc cttgtcttat aagaataata taatatatac
     1741 tactgaaata aggtagtata tattactagt aatggtagta tgttttatat gcataaggta
     1801 gttcttaatt tattttttgt tttgacactg gtagtactta attatcttta attttaaaac
     1861 taaattttat tcatacgttt ttctggtagt
//
LOCUS       DARBEC010000184         1883 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.191, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000184 DARBEC010000000
VERSION     DARBEC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.191"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(2..259)
                     /locus_tag="SCL80_002812"
     CDS             complement(2..259)
                     /locus_tag="SCL80_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108635.1"
                     /translation="MSTPSRGIKNTPVLTGVWVTDRLDFNWCQKLMSRKGDEILAIYG
                     YSRISTSNQDYKTQIQTLENAGAEIIFLENHWIIEHLWKYF"
     gene            complement(327..782)
                     /gene="tnpA"
                     /locus_tag="SCL80_002813"
     CDS             complement(327..782)
                     /gene="tnpA"
                     /locus_tag="SCL80_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEG1108636.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1025..1883
                     /locus_tag="SCL80_002814"
                     /pseudo
     CDS             1025..1883
                     /locus_tag="SCL80_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          597 a          366 c          354 g          566 t
ORIGIN      
        1 ctcaaaaata cttccaaagg tgttcgataa tccagtgatt ttctaggaag attatttctg
       61 cgccagcatt ttctaatgtt tgaatttgtg ttttgtaatc ttggttgctt gtgctaattc
      121 ttgaatagcc gtaaattgcc aaaatttcat caccctttct ggacataagt ttctgacacc
      181 agttaaaatc aagtctatca gtaacccata cacccgtgag gacgggtgta tttttaatcc
      241 ccctagaagg ggtcgacact cgctgcccct taaaagggga tttttaaaag accgccaact
      301 gctcatcttc actcacttac ttctctttat tttttaaatg gttctacgta ctctctttta
      361 cttataatat atcgaatgcg atcatcagct tcttgttcac ggatgtattt ttcatttgtc
      421 ttttgattca atccgactgt actcacatag tatctcttcg accaaaagga tttgtttccg
      481 tatttatatt tgagatttgc gtgctgctcg tggattagta ccgcactacg acctttcaga
      541 taccccataa aactggatat acatattttt ggtggtactc gaacaagcat gtgaatatga
      601 tctagcattg catgtgcttc tatgatttcc acatctttca tttcgcacaa cttcctaagt
      661 atttttccaa gatcttttcg taattgccca tagattactt ttcgttgata ttttggtgta
      721 aaaataatat gatacttaca attccaacga gtatatgcta aacttttatc gtcatttgac
      781 atgaaaaaac tcctttcgat ctgatgagcg gttggtagcc actcactagt atatcgaaag
      841 gagtttttat cgctaaagat acttcttcca cgcgtagaaa cgcgcggttt tcttgtttcg
      901 cacgctttac gcacaaaact gactgaagtc attaagaaaa taaaagggtc taaaactttt
      961 aagttttgta ccgaccccca aaagttagac caaaaaatct aacttttgga ggtcggtatt
     1021 ttttatggct aagtatactt ttgaacttaa attaaagatc gttcatgatt acttggatgg
     1081 taaaggcgga agtgattatc tggcaaagaa atattccatt aaggctccat cacaagtgaa
     1141 gcgctggatc aatgcctatc aagaatttgg tgaagagggt ctagtaagaa agcgacaaaa
     1201 gaaaaaatat tctgttcaat ttaagctgga tgcgatagag ttatatctaa caagtgagct
     1261 gtcctatcgt gaagttgcca acacacttaa catgaacaac cctaatttga tcgctagttg
     1321 gatgcgtcaa ttccgtgagg gtgggatcga tggactctca aaaacgaaag gatgtccacc
     1381 tatcttgtct aagaaaaatg aacccaaaaa taagaaaaaa tctacaacta aagcaacgtc
     1441 taaagaacgt gaacgcatcg aggagttgga gaaacgagtc cgttcccttc aaattgaaaa
     1501 tgccttttta aaagaattga ggaagctgcg aaaacaggaa gcccaacaac gacgaatgaa
     1561 gcaatcgcac gaatcatcgc aagcctccga ggaccgttta aattagttga gcttctagaa
     1621 actttaaagt tccctaaggc aatctttatg tactggcaaa aacgattgga ccgtaaaaat
     1681 ccagatcaag agattgaagc ggaaatgctt aaagttcggg agaagcacaa ggattatggc
     1741 tgtttacgga tgaccaatga actaagaaac cgcggtttct ctatcaataa aaagaaagtt
     1801 cagcgcctga tcaagaagct aggaataaaa gtaacgacat acacacgtaa atcacggcgc
     1861 tacaattctt atcgaggaca agt
//
LOCUS       DARBEC010000185         1796 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.192, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000185 DARBEC010000000
VERSION     DARBEC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1796)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.192"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            474..569
                     /locus_tag="SCL80_002815"
     CDS             474..569
                     /locus_tag="SCL80_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEG1108637.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(872..1117)
                     /locus_tag="SCL80_002816"
     CDS             complement(872..1117)
                     /locus_tag="SCL80_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108638.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            1462..1746
                     /locus_tag="SCL80_002817"
     CDS             1462..1746
                     /locus_tag="SCL80_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108639.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
BASE COUNT          573 a          347 c          292 g          584 t
ORIGIN      
        1 cttctgtatc cgttgggaga gtaaggctca actgccccct aaaaagggtt ctaaaaaatc
       61 tttaaaaaat aaataaaata aacgcgcgcg cgcgagcaaa atttcccgcc aaaaaatcac
      121 ccacttttta aaataaacag gttcgcgcat gatattaaac tgccaatcta gctccctatg
      181 gtcgctgtaa gaaggagtga tcgctacgct ctcacatttt acttgcagca gtcgccttta
      241 ggctccttcg ccaaaaataa ttcattaggg actcctttgt ggcgtcctga gagctcgtct
      301 aagccgttct aagacaagat tcgataaaaa ctatgtcctt cgttctaaag cgccttacag
      361 gcggtcaggg caagccctaa caaccccaag gcaataaaaa acttaggctt atttttgctt
      421 ttttattgta taattgattg tcgcctatcc gacagtcttg cgactggaag gaggtgtgtt
      481 tcgtgttcac taactttatt tgctcccttc ttgttgggct tttaagcgct ttgttcagtt
      541 actggttgaa taaccgaaac aagaagtaaa acttaattaa ggcgactcag cccacaaaaa
      601 aagccccaga gaatccgacc tctctggggc tttcgtgtgt ttcgtgttca cttatttgca
      661 atcaaattat aacagaaata aatatttatt tcaaaaatat cctacaaaag tctaaaatcc
      721 gtaattattt cttatatgca acatttacaa ataaagtcgc tctgagagct cgtatttttc
      781 gatttaagac gttttattta gtttttggtg tattttcatt ggtccaagct aaaaaactct
      841 tagaagcgat tttagagcca tttaaaccca cttaattata ttctccatga aattcgctcc
      901 atgcgtcaaa agctgcgtct gaaattccgc cgtcttcgtt cactagttgc attaattttt
      961 ccaacgatcg ttttctttcg ggtgtcatat ttttatattc caacaatttt tcgtctgtta
     1021 aatcgaactt gattgcttga attgcttgct ctaatttttc taagttttga aaatattcgt
     1081 aaggaaattt ttccattttt tctttctcca gtctcacaat ttttctagaa ataaaatcac
     1141 ttctttgccg tctatactct caaaaaaaat gtcgtttgaa aaactaaata aatttttgat
     1201 cgtgcatttt taaatttggt tatcgattac acgtgcagca ctcccccccg aattttaaag
     1261 agcataagga aacgtgtacc cactttctaa aaaaagtggg tagataggct gtgcctatca
     1321 cgtttctcag aaaaaaattt gatatcactt tttcgccatc gtgggtagct tatgtatagc
     1381 attttggcaa aaaacagcta tacaaatata gtatttgtat ggtatacata aattatacat
     1441 aattgcaaaa ggagattttt tatggacatt ttagttcgga atattgatcc aaagtttgtt
     1501 gcaacaatag acaaacgttg ccaagaactc acgaataaaa caggagaaaa atggagtcga
     1561 aataaatatc taaaagtttt aatcgaaagt gattttgacc gtcaacttac gacttacaaa
     1621 aaagaccaat tcgatttatt gctcgataaa tttattgcta tccaaacaga aaatactcaa
     1681 atattaagcg agtatgtcta cacaaataat caaatcattg aaatgcttat cggaggaatg
     1741 atataaccat gagaaaaact tctgtccaaa aacatgttac tttgtaccct agtcat
//
LOCUS       DARBEC010000186         1792 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.193, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000186 DARBEC010000000
VERSION     DARBEC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1792)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1792)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1792
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.193"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..258
                     /locus_tag="SCL80_002818"
     CDS             <1..258
                     /locus_tag="SCL80_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partitioning protein ParA"
                     /protein_id="HEG1108640.1"
                     /translation="MIGFVPYLVDTDSATIKSNLEELYKQHKEDNLVFQNIIKRSNKV
                     STWSKNGITEHKGYDKKVLSMYKNVFFEMLERIIQLENEKE"
     gene            356..565
                     /locus_tag="SCL80_002819"
     CDS             356..565
                     /locus_tag="SCL80_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEG1108641.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            582..854
                     /locus_tag="SCL80_002820"
     CDS             582..854
                     /locus_tag="SCL80_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEG1108642.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEV
                     NLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYSKQVLNKEKVA"
     gene            856..1719
                     /locus_tag="SCL80_002821"
     CDS             856..1719
                     /locus_tag="SCL80_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin zeta"
                     /protein_id="HEG1108643.1"
                     /translation="MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAI
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLEKELNRKVSGKEIQPTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTP
                     PIPKTPKLPGI"
BASE COUNT          726 a          282 c          322 g          462 t
ORIGIN      
        1 atgatcggtt ttgttcctta tttagttgat acggacagcg caacgataaa atcaaacctg
       61 gaagaactgt ataagcaaca taaagaagat aacttggttt tccaaaatat tatcaagcga
      121 agtaataaag taagtacctg gtctaaaaac ggcattacag agcataaagg ctatgacaaa
      181 aaagttttat ccatgtataa gaacgtattt tttgaaatgc ttgagcgaat tattcaatta
      241 gaaaacgaaa aagaatagaa tcacaaatca caagtgatta atcacaaatc acttgtgatt
      301 tgtgattgtt gatgataaaa taagagtaag aagaaataga aagaagtgag tgattgtggg
      361 aaatttaggc gcacaaaaag caaaacgaaa tgatacacca atcagtgcaa aaaaagatat
      421 aatgggagat aagacggttc gtgttcgtgc tgacttgcac catatcataa aaatcgaaac
      481 agcaaagaat ggcggaaacg taaaagaagt tatggatcaa gccttagaag aatatatacg
      541 gaaatattta cctgacaaac tttaaaagga gtgaaaatga aatggcagtt acgtatgaaa
      601 aaacatttga aatagagatc attaacgaat tatcggcaag cgtttataat cgagtattaa
      661 actatgtttt gaaccatgaa ttaaataaaa atgactctca attattggaa gtcaatttat
      721 taaaccaatt aaagcttgca aaacgtgtaa atctttttga ttattcttta gaagaattac
      781 aagccgttca tgagtattgg cggtcaatga atcgttactc aaaacaagtt ttgaataaag
      841 agaaagtggc ttaatatggc aaatatagtc aattttactg acaaacaatt tgagaatcgc
      901 ttaaatgata atttagaaga attgattcaa ggaaaaaaag cggttgaatc gccaaccgct
      961 tttttacttg gtgggcaacc agggtcaggg aaaaccagtt tgcgatcagc aatttttgaa
     1021 gaaacacaag ggaatgttat tgtcattgat aatgatacct ttaaacaaca gcaccctaat
     1081 tttgatgaac tagtgaaact ttatgaaaaa gacgtagtaa aacacgttac cccttattct
     1141 aatcgcatga cagaagcgat cataagccgt ttgagcgatc aagggtataa tttggtgatc
     1201 gaaggtacag gacgaacaac agacgttcct attcaaaccg caacaatgct tcaagccaaa
     1261 ggttatgaaa caaaaatgta tgtcatggca gtacctaaaa tcaactcata tttaggaaca
     1321 attgaacgat atgaaaccat gtatgcagat gatccaatga cagccagggc aacaccaaaa
     1381 caagcgcatg atattgttgt caaaaactta ccgaccaatt tagaaaccct tcataaaacg
     1441 ggcttattta gcgatataag gctatataac agagaaggag taaaactcta ttcaagtttg
     1501 gaaacacctt ctattagtcc aaaagaaacc ttagaaaaag aattgaatcg taaagtatca
     1561 gggaaagaaa ttcaaccgac tttagaacga atagagcaaa aaatggttct aaataaacac
     1621 caagagacac ctgaatttaa agcaattcaa caaaaattgg aaagcttgca gccacctaca
     1681 ccaccaatac ccaaaacacc taaacttcca ggtatttaaa ccttaaaaaa ggaaaagagt
     1741 agttaccaaa aaacggtaac tactcttttt ttataaaaac atttcctcat gt
//
LOCUS       DARBEC010000187         1738 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.195, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000187 DARBEC010000000
VERSION     DARBEC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.195"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="SCL80_002822"
                     /pseudo
     CDS             1..1234
                     /gene="aph(2'')-I"
                     /locus_tag="SCL80_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
     gene            1218..1610
                     /locus_tag="SCL80_002823"
     CDS             1218..1610
                     /locus_tag="SCL80_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108644.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
BASE COUNT          715 a          133 c          297 g          593 t
ORIGIN      
        1 atcattaaaa aaacattata cagagccttg ggaagatgaa gtttttagag taattattga
       61 atataacaat gttcctattg gatatggaca aatatataaa atgtatgatg agttatatac
      121 tgattatcat tatccaaaaa ctgatgagat agtctatggt atggatcaat ttataggaga
      181 gccaaattat tggagtaaag gaattggtac aagatatatt aaattgattt ttgaattttt
      241 gaaaaaagaa agaaatgcta atgcagttat tttagaccct cataaaaata atccaagagc
      301 aataagggca taccaaaaat ctggttttag aattattgaa gatttgccag aacatgaatt
      361 acacgagggc aaaaaagaag attgttattt aatggaatat agatatgatg ataatgccac
      421 aaatgttaag gcaatgaaat atttaattga gcattacttt gataatttca aagtagatag
      481 tattgaaata atcggtagtg gttatgatag tgtggcatat ttagttaata atgaatacat
      541 ttttaaaaca aaatttagta ctaataagaa aaaaggttat gcaaaagaaa aagcaatata
      601 taatttttta aatacaaatt tagaaactaa tgtaaaaatt cctaatattg aatattcgta
      661 tattagtgat gaattatcta tactaggtta taaagaaatt aaaggaactt ttttaacacc
      721 agaaatttat tctactatgt cagaagaaga acaaaatttg ttaaaacgag atattgccag
      781 ttttttaaga caaatgcacg gtttagatta tacagatatt agtgaatgta ctattgataa
      841 taaacaaaat gtattagaag agtatatatt gttgcgtgaa actatttata atgatttaac
      901 tgatatagaa aaagattata tagaaagttt tatggaaaga ctaaatgcaa caacagtttt
      961 tgagggtaaa aagtgtttat gccataatga ttttagttgt aatcatctat tgttagatgg
     1021 caataataga ttaactggaa taattgattt tggagattct ggaattatag atgaatattg
     1081 tgattttata tacttacttg aagatagtga agaagaaata ggaacaaatt ttggagaaga
     1141 tatattaaga atgtatggaa atatagatat tgagaaagca aaagaatatc aagatatagt
     1201 tgaagaatat tatcctattg aaactattgt ttattgatga taatatggtt tttgatattg
     1261 ataatttaaa aggatttctt aatgatacca gttcatttgg gtttatagct aaagaaaata
     1321 ataaaattat aggatttgca tattgctata cacttttaag acctgatgga aaaacaatgt
     1381 tttatttaca ctcaatagga atgttaccta actatcaaga caaaggttat ggttcaaaat
     1441 tattatcttt tattaaggaa tattctaaag agattggttg ttctgaaatg tttttaataa
     1501 ctgataaagg taatcctaga gcttgccatg tatatgaaaa attaggtggt aaaaatgatt
     1561 ataaagatga aatagtatat gtatatgatt atgaaaaagg tgataaataa atgaatatag
     1621 ttgaaaatga aatatgtata agaactttaa tagatgatga ttttcctttg atgttaaaat
     1681 ggttaactga tgaaagagta ttagaatttt atgatggtag agataaaaaa tatacatt
//
LOCUS       DARBEC010000188         1717 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.196, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000188 DARBEC010000000
VERSION     DARBEC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.196"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(154..531)
                     /locus_tag="SCL80_002824"
     CDS             complement(154..531)
                     /locus_tag="SCL80_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108645.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(831..1712)
                     /gene="galU"
                     /locus_tag="SCL80_002825"
     CDS             complement(831..1712)
                     /gene="galU"
                     /locus_tag="SCL80_002825"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEG1108646.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT          484 a          380 c          252 g          601 t
ORIGIN      
        1 aaaaaggaat ggcttatccc gatacaacaa gcattccttt aaaaaagatt cttttatata
       61 agtgaaaaaa gcttttttcc accaattaaa taaatctctc atctcaatag tttatccatt
      121 attttatctt tttttgcgta tacctactaa atattaccgt cttcttctcg ttcttctact
      181 ttgttcgaga gaatctgtac tagaaaaagt agttgtatgt ctaccttttc tgcctatccg
      241 agactttctt ttcatatatg ggatataaat gattagatac agtacagcaa aaaataaagc
      301 aaatacgcaa gcttcccaac tacctaacgc caattcaacc atcaaggtag caaccccaac
      361 ggatgctgca taccagataa aaatcagaaa cgtttctttt tttctgacca tgcgaaacat
      421 gataaataga ctaataagca atagtggcaa cacaaatatc caattgcttc cttccatcag
      481 ccacttgagt atatcaagca gcgactgtgc aacagcgact aattttccca tagtgaacac
      541 acaccttttt ttataattaa aaaattcgcc tttttattct actatgaatc ttctggcttg
      601 gctttcattt ctcttatgta atcattttct tttatacaac ttaaaaaaaa attaaaccat
      661 tagcataata ttttctgaat ctattcacga aagtcttttt ctatctttcg ttttccacga
      721 ctcaactgat ctagagtcaa aaaactaaaa cgacagttgc gcaaatgacg cacaagctgc
      781 cgctttaata acaaatagcc tttcattctc agcaggccac ttatttccag ttaactttct
      841 gttcttagct ttttactcaa ctggataata tatgcactta atgaagtccc aatttccgaa
      901 tgttgaagtc catattcaat atttgtcttc atatatccta acttatcgcc tacgtcgtat
      961 ctttttcctt taaactctct cgcatagacc gcctctgtct ggttaagagt ctgaagtgca
     1021 tccgttagct gtatctcgtt tcctgctcct ggttcttgag tttctaatat ctcaaatatt
     1081 ttaggagtca gtagataacg tccgataatg gctaaattac ttggtgcttt tcccatttca
     1141 ggtttttcta caaacccttt tactctgcaa cgaccctttt cgctatctgt tttaggatca
     1201 ataattccgt atttagatgt ttcattttct ggtacacgca tgacggcaac agcggaagct
     1261 tgtgtttctt cataatccat catcaattgt tgagtcaatg ggatatcatc tttcatcaaa
     1321 tcatccccca gcatgacgac aaacggttca tttcctataa atgcttttgc ttgaagcacg
     1381 gcatccccca gtcctcttgg atgagattga cggatgaagt gaagattgac ttgagtcgtt
     1441 tcttcaacta aagcgagtaa ttctgttttt tccttttccc gcaaattgtt ttccaattcg
     1501 atatttgcat caaagtgatc ttcaatgggt cttttttctt ttcccgttac gatcaagatg
     1561 tcttcgattc ccgaatccaa tgcttcttcc acaataaact ggatcgttgg tttatccacg
     1621 attggaagca tttcttttgc catggctttt gttgccggca aaaaacgtgt tcctagccca
     1681 gcggcaggaa tgactgcttt tttgaccttc acttgct
//
LOCUS       DARBEC010000189         1621 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.197, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000189 DARBEC010000000
VERSION     DARBEC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.197"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            186..1274
                     /locus_tag="SCL80_002826"
     CDS             186..1274
                     /locus_tag="SCL80_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108647.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            1271..>1621
                     /locus_tag="SCL80_002827"
     CDS             1271..>1621
                     /locus_tag="SCL80_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEG1108648.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKY"
BASE COUNT          629 a          182 c          270 g          540 t
ORIGIN      
        1 ttgacggata atacgctcac ttatccagac actcaaatga tttttctctt agtacctatg
       61 atagtttcgt gggttgataa tcaacaacga atatcggaat agatagctaa agtatatttt
      121 aattatgaat ttataattag ttgtaaaagt taatatttaa cggacaggaa gtagggtgaa
      181 aaaatttgaa aaaaatttta gtgttagcaa atacatatta tcaaataata gtagcaatac
      241 aaatgaaaaa tacaattttt tccgatgagt atgttgaaat tattatttct gatcattcta
      301 aaaacactaa agaaattgcg aaaaaaatag aagaaaaaaa agtgtttgat ggctgtcatt
      361 atttagaaag caaatctttt gatgataata ttgggtattt tcaaaagatt aaggactgtc
      421 ttcagatttc ttgtggtaaa tcaaatcgat atgcctatta tttgaaaaac tgtaaaagtg
      481 aatattttga tgaatttatt tgtttttcaa tgggagaggt ttggttggaa ggaatatatg
      541 cttgcttata tcgaaataat aaaaatgtac aggtatcaat tttagaagaa ggtattttaa
      601 gctatggagt tgacattatt ccaacagcaa gaacaaaagc tatcgcagta ctcaattact
      661 taaaaaaaag gactccacta tataacaaaa gctataattt ttattgtttt tttcctaata
      721 tatatacagg aagagagaat acaatacaaa ttccattagt tacagctggg tcaagaaccg
      781 cattgcaact ttcggatatt tttaacccta tgatagatat ttataaagaa aaaaaatata
      841 tattttttac gtctgtttat gattttgagg gcggagaacc tgtcggagaa tatgaattag
      901 tatgcaaaat agcagactta gtaggaaaag ataatctaat tgttaagacg catcctagag
      961 atgtaagaac aatttataaa gataatggat ttatcgagga taaaaattca tcaatacctt
     1021 gggaggcgat tcaattgtca ggcgatttta acgataaaat atttatgaca attaattcag
     1081 gaagtgttat ttcaggtagt acaatgtctg caaaacctgt acgcacgtat tatatgtata
     1141 aattgtgtaa catagaagga aatccgtctt gcaaaaagaa tgcgtatgat attgagcgat
     1201 tgcttaagca aaaaacgatg aaaaacattt taaagaatgt gaaaatagca gaaagcttag
     1261 atgatatact atgagcaaaa ctaaatattt aattaaaaat atgggaatat taacaataag
     1321 taatttttct tcaaaaattt tagttttttt gttagtccct ttatatacaa gtgtattatc
     1381 cacaacagaa tatggcacat atgatttgac aatttcaaca gccacattat tgtttccaat
     1441 tttaacctta aacattgtag atgctgtgat gcgcttttta atggataaag ataaaaataa
     1501 aaaaaatata gcatctatag cgattaaagt tatattttta agcgtgatag tctttggatc
     1561 cgtaatgtat ttgttaaata ttacaaaatt atggcaagat gtttatggat tagaaaaata
     1621 t
//
LOCUS       DARBEC010000190         1619 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.198, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000190 DARBEC010000000
VERSION     DARBEC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1619)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1619)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1619
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.198"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            101..868
                     /locus_tag="SCL80_002828"
     CDS             101..868
                     /locus_tag="SCL80_002828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108649.1"
                     /translation="MKKDELTKYNEEFLATIDMKMDLKSYELYQLLFSYISPTKLKKT
                     GVVDLPVKAIINYMDVDQNLETELKTIVNTLQRQAVYEISNLDKKYKTYTTVSAIDRI
                     HWDLEEHSVSFSFSEDVIPLIVSIKKKYPFFVFEDLASFQKKYSIVLYKYLAMYYKKY
                     LIAKNKGDFEKAEIYKNPLIEIVKIREITKTENEYKTISNFDRRVLRESVEEINEKSM
                     LNIDFAKVRKGRNYNFAVQFNISSEFDEKTYREKKLG"
     gene            1060..1458
                     /locus_tag="SCL80_002829"
     CDS             1060..1458
                     /locus_tag="SCL80_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HEG1108650.1"
                     /translation="MKYFFCAVFEWKGNEYAVKFPDFPDLYTHGETLEDAFEMAQEVL
                     EGYLVLLELQEKEIPSPTPVQELNARIKRSENVNLFVVKANTKVAKARNKQNYDRKSV
                     TVEHYLNVLAAEENINLSKALNDLLREKLI"
BASE COUNT          591 a          210 c          313 g          505 t
ORIGIN      
        1 aatactgtta aatatctcga aaaatcaatt acgagttatt tgccttatgt gaacctttat
       61 gattaacaac tgaaaaaatg cgtgaaggaa ggaaaaactt ttgaaaaaag atgaactgac
      121 aaagtacaat gaggagttct tagcaacaat tgacatgaaa atggatttaa agtcgtatga
      181 actttatcaa ttgctgttca gttatatctc acccacaaag ctaaagaaaa caggcgtagt
      241 agatttacct gttaaagcga ttattaatta catggatgtt gatcaaaatc ttgaaacgga
      301 actaaaaacc atagttaaca ctttgcaaag acaagcagtt tatgaaattt cgaatctaga
      361 caaaaaatat aagacgtata ctactgtatc tgctattgat cggattcatt gggatttaga
      421 agagcatagt gtatctttca gtttttcaga agatgtaatt cccttaattg tctcaatcaa
      481 aaagaagtat ccgttctttg tttttgaaga cttagcatct tttcaaaaaa aatatagcat
      541 cgtgttatac aaatatttgg cgatgtatta taaaaaatat cttatcgcca aaaataaagg
      601 agattttgag aaagctgaaa tatataagaa tcccctgatt gaaatagtta aaattcgtga
      661 gattacgaaa acagaaaatg agtataaaac aatatcgaat tttgatagac gtgttttaag
      721 ggaatcagtt gaggaaatta atgaaaaatc aatgctgaat attgattttg ctaaagttag
      781 aaaaggcaga aattacaatt ttgctgtcca gtttaatatt tctagtgagt ttgatgaaaa
      841 aacttataga gaaaaaaaat taggttaatg caaaatatat cttactcgtt tgaagatgct
      901 tcagacgtat gctaagatat atagtgcctt gggagcaagg tattaatctc ctttagaggt
      961 taattagctt gtgatcgcct atcaattttt ttggtaggcg attctttttt ttgaaacaaa
     1021 aaagcaaaaa ttatttttta attagagagg tgcaatgcga tgaaatactt tttttgtgca
     1081 gtattcgaat ggaaaggaaa tgaatatgca gttaaattcc cagattttcc agacttatat
     1141 acacatggtg aaactttaga agatgctttt gagatggcgc aagaagtatt ggaaggatac
     1201 ttggtgcttt tagagttgca agaaaaagaa attccatcgc ctacgcctgt tcaagagtta
     1261 aatgcaagaa tcaagcgatc tgagaatgtt aatttatttg tcgtaaaagc taatacgaaa
     1321 gttgctaaag ctcgaaacaa gcaaaattat gatcgaaaat cagtgactgt ggaacactat
     1381 ttaaatgttt tagcggctga agagaatatt aatctgtcga aagctttgaa tgatttgttg
     1441 agagaaaagt tgatttgatg tctctcagat gatcatatta agcaaaaaca gcttgtttta
     1501 ggacgtatgg atttaacaga attaggcaga agtcgcccat cctctatagg tgggtgatga
     1561 atgccgttcg gtatgagggt taccgttggt aactcgaaga ccgacaacta aggcgtatt
//
LOCUS       DARBEC010000191         1609 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.199, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000191 DARBEC010000000
VERSION     DARBEC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.199"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            519..>1609
                     /locus_tag="SCL80_002830"
     CDS             519..>1609
                     /locus_tag="SCL80_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108651.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTG"
BASE COUNT          543 a          158 c          284 g          624 t
ORIGIN      
        1 ccttccaaca gtatataaaa tgagcgtaaa aacggctatt tttgccttaa caacgatttt
       61 tttgtataca aatacgatat gctcattaat tattggaatt tgtagtgctg tttaaattaa
      121 tgaaggtgta attatatgag tgatcatttt gaaaaagttt gttgagaaga tgtgcttcta
      181 gtcatgtgat gggagaaaat aatttaactg ttagagtgaa tagttaaatt attctggtta
      241 tatgaaaaaa gtagtttcta aaataatctt gcagggatta acttaaagaa attgagagaa
      301 tagtttcttt gaaatttcta tatacaatga gaagctgaaa tttactttta tttgatgact
      361 ggaaatagaa attaaatata ttgtattgtt acattaaaga aaacttgtat ggaaaaatca
      421 gtacaatacc attatttaat gttcttcact taaagcgcaa aaaatatcag tttattaaac
      481 tcgtatatag aacaaatata tgtagacggg gcgaaacgat gagtgtaaaa aaaatgctat
      541 ttatggtaag tacaataatt gtattaacat cccgattttg gggaattaat actagtttat
      601 ctatgcgata ttttattatg gcaatatggg atttatattt tatgttaagc tatttttctt
      661 atcgtgagaa aacatataaa aaatacgtta tcaagaataa cttttggttg actataatgc
      721 cgtttgtgat attttctatc tatactctaa taatatgggg aattggttca aatgcagaat
      781 ttgaaaattt tataaagtta tgttccacgt tgctttattt aattcttgca tatggttacg
      841 catgtacagc attttactta tttaaggcag aaggaattga ttttattttt tggtcaggtg
      901 ttatttcata tatgagtggt tcagtttttt atttaattgt atcttatggt ttaaatggtt
      961 taatttctta tataaaaagt cttataacag gtgaaacaaa tactgcaaat tttgctatgg
     1021 aggtacatga tttaactttt gcgatggggg tttttttcct ttattacgta ttttttgaaa
     1081 ataaaaatac aaaaaaacat aagataaaaa ttatcgtatc tttaatttta attttcatgg
     1141 gattgaagag aattgaactg ttagctttaa gtggtgcaat tctagtgtat tatgttttat
     1201 taaagtgggg aaaaaagatt caaatccgtg cattagtgtg tactatttgt gcaactgtta
     1261 tttcgttagt gtttatttac attatagata caggaatttt ggcattgctt atgtcaaatt
     1321 tgggcattac agatgacggt gcgaggcttt cattttatct atattcatct aaattttatg
     1381 aacttggttt aggatatgta ggaacaggat ggacatggtt ttcaaaatac ttttttaatc
     1441 tttatttatc gcactttagg ttaaatggtg gaagaattgc agcaagccta cacagtgata
     1501 tattaactat ttatatagaa gttggatgta tagtttttat tatatggatg ttttggttat
     1561 tttttgtgaa accgataaga ctaactaaaa aatttggatt gaaaacagg
//
LOCUS       DARBEC010000192         1550 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.200, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000192 DARBEC010000000
VERSION     DARBEC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1550
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.200"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            219..1388
                     /locus_tag="SCL80_002831"
     CDS             219..1388
                     /locus_tag="SCL80_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1108652.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
BASE COUNT          572 a          222 c          247 g          509 t
ORIGIN      
        1 tttatagcaa agaaagttta ataagagttt ggacctagca aacaatatta atgaggtcat
       61 taaatcttat tatctttagt gatataacga aatatctaca cagacaaaat gatagcgtaa
      121 aatattttgt gtaaatagat atttagggta gaaaaaaaga aagtccattc tgtaaaatta
      181 aagttaccac aaccgaaatt ttataggagg actttctcat gcccaatttt actacagaaa
      241 ttatggaaac actaatcaat aaaggtgatt tagatgaatt atttcgatgt catttagaat
      301 tagctgttaa ttcattattg caggctgaat taacagcttt tcttgattat gaaaaatatg
      361 atcgtgctgg gttcaattca ggtaattccc gtaatgggaa ttattcacgt tcattcagaa
      421 cagagtatgg agaattaaat ttggtaattc ctagagatag aaatggtaag ttttctcaac
      481 aaactttacc tgcttataaa agaaccaatg actctttaga aaccactatt attcagctat
      541 ttcaaaaagg aattactatg tctgaaatct ctgaattgat tgaaaaaatg tacgggcatc
      601 actatacgcc acaaaccatt tcaaatatga ctaaaatagt ttctgaagat attattgcct
      661 ttaaagaaag gtccttagaa tccagatact ctgtcatttt tatggatgct actcacattc
      721 ctttaaaaag acaaactgtt tcaaaagaag cagtttatat tgtaattggc attcgattag
      781 atggaaccaa agaggttctt gggtttagtc ttgctccaac tgaatctgct tatgtttgga
      841 aagagattct tcaagaccta aaagaccgtg gtttaaaaga ggttttatta gtcgtaactg
      901 atggtttaag tggcattaac gatagtattc atagtgttta tccaaatgct caatttcaac
      961 aatgttgtgt ccatatatct agaaatatcg ctcataaggt tcgtgttagt gatcgacaag
     1021 aaatctgtag cgatttcaaa ttagtttatc aagcaagttc aaaagaagaa gcaaataatc
     1081 aaataaggtt tatgatagat aaatggaaaa agcagtatcc acgagtggtt aaattgctca
     1141 tgaatcctgc tatattaacc ttttataatt tccctccgtc aatcagaaga actatttatt
     1201 caactaattt gattgaaggt tttaacaaac aattaaagaa atataccaaa agaaaagagc
     1261 aatttcctaa tgaagaatct ctggagagat ttctagtatc tcaattcaac aactataatc
     1321 aaaagtttct atgccgagtt cataaaggtt ttaaagaaat acatgataca ttagaatcaa
     1381 tgttttaact tgatcaacag aatgggcttt tccatttaca caaaattatt gacgctcccg
     1441 acaaaattat gatcttgcgt aaagttataa aatattaata aaaatatttt attaatgaaa
     1501 agcttggtag cacggttatt catatgctaa tttatttcac tttatagtaa
//
LOCUS       DARBEC010000193         1541 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.201, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000193 DARBEC010000000
VERSION     DARBEC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1541)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1541)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1541
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.201"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..221
                     /locus_tag="SCL80_002832"
     CDS             <1..221
                     /locus_tag="SCL80_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEG1108653.1"
                     /translation="NKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIITGF
                     GAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            218..1237
                     /locus_tag="SCL80_002833"
     CDS             218..1237
                     /locus_tag="SCL80_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEG1108654.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGANEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAAN
                     PTLSDGTNHSHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIY
                     RPDVNQAYNTSGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPKR"
     gene            1426..1499
                     /locus_tag="SCL80_002834"
     tRNA            1426..1499
                     /locus_tag="SCL80_002834"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1460..1462,aa:Ile,seq:cat)
BASE COUNT          498 a          288 c          321 g          434 t
ORIGIN      
        1 ataataaaac gttcgaagta ctaaaatggt tcgcactggt aattattccc gcactagcta
       61 ctttcgtggg gttagttggt aaagcgctca attggcagta cacagatatc tgtgttgtca
      121 tcattactgg ttttggcgcg tttttaggga gtgtgttggg tgtatcaaat cgaacctaca
      181 aaatgttctc ggctgaaagc gaagaaggag gaaacaaatg aaaaagaaaa ttactattac
      241 tgcgatgagc ctgttaacgg ctcttttttt attgccaatt aacggatttg cctatactat
      301 caacaatgaa tttaatttgg gcgcaaatga aggtagctca caagtagcaa ataatcagta
      361 tattttactg catgaaacgg ctaatgaaac agcaacagga cgcaatgaag cgcagtatat
      421 gcaacgttca tggactagcg cttatactgc ttatattgtg ggagacggcg gaattgttta
      481 ccaagtcgga caacctggtt atgtacagta cggtgctggt tcatatgcta atgccaatag
      541 tcctgtgcag attgagttac aacacacaca tgataaagca acttttgaaa aaaactataa
      601 agcatacgtt gaattggcta gagattcagc aatgaaatat ggtattccat taacattaga
      661 cacgccttat aaccaaccag gaatcaaatc acatttatgg gtaactcaaa acatttgggg
      721 cgatcacact gatccgtatg gatacttatc agaaatgggt gtaagtaagg aaaaactggc
      781 ttacgattta gctcatggat ttacagacga aaacccaacg acttctgaga acaagcctgt
      841 cattgatcca acccgagcag gtgcagcaaa tccaacttta tcagatggaa cgaaccattc
      901 tcacattgat cagtttggag aaatcgaaaa tgcgaactta cacgtcgctg gatggcacat
      961 cgctaactat aagtacgagt atattttcat tatggactac aatactggga aagaattagc
     1021 tcgagtaaga gctgatggaa tttatagacc ggatgtaaat caagcttata atacttctgg
     1081 aaatgttggc tatcatgtat cttttaacat gcgtaacttc cctaataaga aagtctatgt
     1141 catgatgcgg gcaacgaatg atccagaggg aaacactaaa ggcggtgcgc aagatttcca
     1201 tgacaaacgt tggtatttaa atattcctaa acgataaaaa tagctcctcg ttgaggagca
     1261 ttacataact atattgacaa ctataaaaat cattcgataa aatagtgatg ttatcgcata
     1321 tcttcactat cacccataat agtcacactc caagctatgc gataacaggt ttgttgccac
     1381 acattctact ggttgattgt ttatggcttt atgtggcaac aaccagtacc cttagctcag
     1441 ttggtcagag cagacggctc ataaccgtcc ggtcgtaggt tcgagtccta cagggtacat
     1501 taacgtagcc atttgaatcg ttctgtgtta gaattttttt g
//
LOCUS       DARBEC010000194         1507 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.202, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000194 DARBEC010000000
VERSION     DARBEC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.202"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..340)
                     /locus_tag="SCL80_002835"
     CDS             complement(<1..340)
                     /locus_tag="SCL80_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108655.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNP"
     gene            complement(342..1400)
                     /locus_tag="SCL80_002836"
     CDS             complement(342..1400)
                     /locus_tag="SCL80_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEG1108656.1"
                     /translation="MVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPGLLARQGF
                     IEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQIFYITLSS
                     SRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFSFGTNYAK
                     DFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIWKAALKVI
                     RPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLVSSGIIAF
                     ICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFNKRDVISI
                     VFWYYLGSISYLYLKKEN"
BASE COUNT          587 a          242 c          183 g          495 t
ORIGIN      
        1 ctggattttt tttgtaaaca ttggagaaaa gtatttttgt gaaataattt tccccctgta
       61 taaaaatttc atcataaacg gataaatcat ccgagaaaaa ttgattatgg ttagttaaca
      121 agttaatttt acgtttaaag gaagaaccga ttttccggtg attcacaaaa acaacatcat
      181 taaatagccc taattctttt atacgctcac tatatcttct agcgttttca aattcagaat
      241 aaataaataa atctcctttt gattctcggt aaaaattttg ataaacattt aatatattaa
      301 tcaaatgtaa aggagtccaa gcaatgaaag ctgtcttcat attaattctc cttttttaaa
      361 tacaagtaag aaatgcttcc caaataatac cagaaaacaa ttgaaatgac atcgcgctta
      421 ttgaaaagta catgagcctc caccatttca tctataaaaa tagatagtat aaaaattaaa
      481 acaataccaa gaaacgcata attcacttta cttctaaaaa atgttttgag taaactaaag
      541 atatttaaca catagaaagc atagatacat ataaaagcaa ttataccact tgatacaagt
      601 acttgaagaa atccattatg catgtttcca tgtgctcttt ttagttcatt cacatctgta
      661 gtatcaagat ctactttttt caaaaatttt gcatcgctat tcccatagaa atcggtctct
      721 ccataaccaa ataacaaagt tggtcttata actttaagcg cagctttcca aatcgtaaaa
      781 cgaccagctg aaatttcgga gttatcacta gaatgctgta atattacttc tttttctgga
      841 agtggagttt tatctgattc atttgactct ataacttcta cttttctatc aatcgataca
      901 ggaattgttg ctaaaaaatc cttagcataa ttagtaccaa aagaaaaata taccaataga
      961 actaataata tgcttaccat ccatttgatt tttacaggta gtacttctct aatgctcttt
     1021 ctaaattttg aattggcaaa caacaaaaga aataatacta aaaaagaaca aattactacc
     1081 tgggcacctc tagacgaaga aagtgtaata taaaatattt gtacgataaa attcatcata
     1141 taaaaaacac gaatcgtttt tgttattttt gaaattaata cactaattaa acttaaaata
     1201 atagaagtat atccaaacat agctccaaca tttggacttg tatagacacc aaataaccga
     1261 tgttcaataa aaccttgtct tgcaagtaat cctggcgttc ctgttgggac atgataagaa
     1321 atcaaaaaaa taaattgata gatagatatt agcgcagcta aaaaaattat gataatgaaa
     1381 acattattaa aatacaccat ttttttcttt ttttcctctt cactttgtga aaagtctgca
     1441 ggaaaaatta ctatcaaagt aatcagcaaa tatccaaaat tataaataga tggaattaaa
     1501 aaatttt
//
LOCUS       DARBEC010000195         1501 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.204, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000195 DARBEC010000000
VERSION     DARBEC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.204"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(368..1171)
                     /locus_tag="SCL80_002837"
     CDS             complement(368..1171)
                     /locus_tag="SCL80_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108657.1"
                     /translation="MSQEVTEKIIDSDVDLVSEQTNAIVRYNNGLNTTALRGFKPVEM
                     DVFWAILSKMKRQGTAEVTFDFNLFRYLVKYDRREKDSFYSVLKALSDKLGTLTYRFE
                     DSDVYEQLWLFQKFRIDKQKETVTIKANEEFEFILNSIGKNFTRFELDNFTKLRSSYT
                     KELYRHLMQYRNKEWRNGYWVVKVEDFRRALSIPSSYRMTNIDKRILEQAKEEFLAPD
                     ENGIQVFETFDYEKIYAKKGRRVDRLRFTFSEPESNLPTISMHNWLEED"
BASE COUNT          454 a          296 c          186 g          565 t
ORIGIN      
        1 aaaaaactta tctaattact ctatattaac ttttaacaaa tagttgactt gtccaaaaat
       61 gttaccttca atctcacaaa gatattaaga tgtttcctac caaaactgat atcaccggac
      121 tttttattct gtgttgtcca aaagtgttac ctttagtcga tcaacaaatt aaaaaaaatt
      181 gtttttttct tgacttgtcc aaaagtgtta cccatagttg ttaaaatttg tatagattag
      241 ccattctttc gaatattatt tagactgaaa cttgcttaca aaaaaacttc ttgcatttat
      301 tgtccaaaag tgttacccat acttttttaa agaagcaggt tgtattaaaa ttacaacctg
      361 cttcttctca atcttcttct aaccaattat gcattgaaat ggttggcaag tttgattctg
      421 gctcactaaa agtgaatctg agccgatcaa ctcttcttcc tttctttgca tagatttttt
      481 cataatcaaa agtttcaaat acctgtattc cgttttcatc aggagctaaa aattcttctt
      541 tcgcctgctc tagtattctt ttgtcaatat ttgtcattct ataacttgaa ggtatactta
      601 atgctcttct aaaatcttca actttgacta cccaataacc atttcgccac tctttatttc
      661 tgtattgcat gagatgacga tacaattctt ttgtgtagct acttcttagt tttgtaaaat
      721 tatcaagttc aaaacgagta aagtttttgc caattgagtt taagataaat tcaaattcct
      781 catttgcctt aattgtaact gtttccttct gtttatctat ccggaatttt tgaaaaagcc
      841 acagctgttc atagacgtct gaatcttcaa aacggtaggt taaagtacct agtttgtcac
      901 tcaaagcttt taacacagaa taaaaactgt ccttttctct tctgtcgtat ttgaccaaat
      961 aacgaaagag gttgaaatca aaagttactt ctgctgttcc ttgacgtttc atttttgata
     1021 aaatagccca aaacacatcc atttcaacag gcttaaatcc tcgcaaagct gttgtattca
     1081 gaccattgtt ataacgcaca attgcatttg tttgttctga tactaaatct acatcactat
     1141 caataatctt ttcggtaact tcttgagaca ttgattactc tccaatcttc tttttgcgct
     1201 cctttaatca tgttaatctg gtactaatta aaggaaacaa ttttttctgc ttgttaccta
     1261 aattaaatca caactaaagg taacagtcaa caaagatttg ttactaatct aattttctat
     1321 tttttcgcct tccaaatcaa gaaaaccgaa ggtaacgtct tatcctagtg tgatacctca
     1381 aatgtccact tttgttacct ttgctgtcca aaagtgttac ccttactgtc cacttttgtt
     1441 acctttactg tccaaaagtg ttacctttat acccctttaa tacttactct cccaagagat
     1501 t
//
LOCUS       DARBEC010000196         1488 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.205, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000196 DARBEC010000000
VERSION     DARBEC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.205"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            288..695
                     /locus_tag="SCL80_002838"
     CDS             288..695
                     /locus_tag="SCL80_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108658.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            709..1110
                     /locus_tag="SCL80_002839"
     CDS             709..1110
                     /locus_tag="SCL80_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108659.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1112..1483
                     /locus_tag="SCL80_002840"
     CDS             1112..1483
                     /locus_tag="SCL80_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108660.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
BASE COUNT          571 a          183 c          259 g          475 t
ORIGIN      
        1 ttgaggggaa gtacatatag ttgttgatat agttgctaat attgttagct aatttgttag
       61 taaaactgta tacgaattta gatagtaaaa aaacagtttg ttccttatat agaggcactt
      121 ttactactga ttaaatgtca gatatgctta caaatatact tataaatatg cttacaaaat
      181 tagctaatat ttccgttgac aaagctttgt tactattgtt aaactaaatt gactagcaaa
      241 agtgaaacat aaatttatta aataaatgta aaaattagga gaaatcaatg tcttatagag
      301 tacaattaat aatttcagaa gatgtagaaa gtcagcaatt tggcactaat gttattaata
      361 aagtaattaa tccagctctt tctattaatg cgcctttaat tcctactgct ttatcatttt
      421 ctgtaacagc tgtggtgtct gagatagagg atacagaaaa gataaaaata gtagaaattg
      481 aagttttaaa caagaatgaa aaacaaattt tttctacggg tgaagtatcg gtaagcttgc
      541 ctcctcaagt taacgatata aactttaata taaacgccag aaatgtcttg gttgaagaag
      601 caggagagca ttatgctgtt gttaaattta atggaactga gattggaagg caaatctttg
      661 atatcaaggt caacaaacca gtggaaaaaa attaaggaga cgatacggat gcttgatata
      721 gtaccatcaa atacacatcg gagtggtagc ttaataaatt ttgctgcgat ttttgcttgc
      781 gcatcggtaa ttctagcatc tccagttgtt gtggatgcaa gttctactcc gaaaactaaa
      841 aatgataatc aacttgtaat tactacaaaa aatgagatta acacaatttc aagtaatgat
      901 ggcaaaattt ttgatgtgat aaatacggtt ttaaaaagaa aagctgaaat caatataaaa
      961 tatgatgaag atttaaactt gtatttcttt gttatcaaaa caacatctga attgttttcc
     1021 tcagattacg atgttttgga tactctcgat aatgtattat ctgactataa atatatgggg
     1081 aaatctgtag tagcaacttt gggggaataa aatgtttgac tttaatgaat atttatctat
     1141 ttccaaacaa attgaatcag atgataaata taattctaaa gaatcttata gaagaactgc
     1201 tgtttcaaga gcatactaca gtgcttttaa aaaatctgat gagtatttga aagaaaatta
     1261 tgatattatt tataatggaa gtagcggaaa aggaagccac caaaccgttt ggaatttgtt
     1321 ttcaacagtt aaggaattga ataccttagg aatacataat agtgggtata gaatgttaga
     1381 gaaaagaaaa tgtgctgact atattcctaa tgaaaccatt acgaaaacag atatggcgct
     1441 gatgaatcga gaagcagaaa aaataataaa caaactcacc taatgaat
//
LOCUS       DARBEC010000197         1479 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.206, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000197 DARBEC010000000
VERSION     DARBEC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1479
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.206"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            131..505
                     /locus_tag="SCL80_002841"
     CDS             131..505
                     /locus_tag="SCL80_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEG1108661.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="SCL80_002842"
     CDS             complement(619..1248)
                     /locus_tag="SCL80_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEG1108662.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="SCL80_002843"
     tRNA            complement(1382..1454)
                     /locus_tag="SCL80_002843"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
BASE COUNT          500 a          225 c          260 g          494 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataatagga
//
LOCUS       DARBEC010000198         1476 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.207, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000198 DARBEC010000000
VERSION     DARBEC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1476)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1476)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1476
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.207"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            338..421
                     /locus_tag="SCL80_002844"
                     /pseudo
     CDS             338..421
                     /locus_tag="SCL80_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            470..553
                     /locus_tag="SCL80_002845"
     CDS             470..553
                     /locus_tag="SCL80_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1108663.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            678..1415
                     /gene="erm(B)"
                     /locus_tag="SCL80_002846"
     CDS             678..1415
                     /gene="erm(B)"
                     /locus_tag="SCL80_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1108664.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            1360..>1476
                     /locus_tag="SCL80_002847"
     CDS             1360..>1476
                     /locus_tag="SCL80_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA adenine methyltransferase"
                     /protein_id="HEG1108665.1"
                     /translation="MSKYCLFLIVIYYLTGGNNSMSRFCKFGKLHVTKGNVDK"
BASE COUNT          525 a          225 c          252 g          474 t
ORIGIN      
        1 cgcaactgtc catactctga tgttttatat cttttctaaa agttcgctag ataggggtcc
       61 cgagcgccta cgaggaattt gtatcgataa gaaatagatt taaaaatttc gctgttattt
      121 tgtacattta acttgacggt gacatctctc tattgtgagt tattagtggt acagttttca
      181 accgttttaa ttataaaaaa gtggtgcatt tttaaattgg cacaaacagg taacggttat
      241 tgcaggtgta tttcttatct atgggtttaa catggatttt atcattaaaa tcatgagtat
      301 tgtccgagag tgattggtct tgcgtatggt taaccctaaa gttatggaaa taagacttag
      361 aagcaaactt aagagtgtgt tgatagtgca ttatcttaaa attttgtata ataggaattg
      421 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      481 ccaaatgcgt tatgtagata aaacatctac tgttttgaaa cagactaaaa acagtgatta
      541 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      601 acagataact aaaattacaa acaaatcgtt taacttctgt atttgtttat agatgtaatc
      661 acttcaggag tgataacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      721 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      781 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      841 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaata
      901 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      961 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
     1021 ttgaaagccg tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
     1081 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
     1141 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
     1201 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
     1261 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
     1321 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1381 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1441 ggaaagttac acgttactaa agggaatgta gataaa
//
LOCUS       DARBEC010000199         1440 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.208, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000199 DARBEC010000000
VERSION     DARBEC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1440
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.208"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            105..1406
                     /locus_tag="SCL80_002848"
     CDS             105..1406
                     /locus_tag="SCL80_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1108666.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          474 a          288 c          264 g          414 t
ORIGIN      
        1 gatagaaaat cattctatcg gtcccttttg agcatgaaaa aacaaaacat acagaagctc
       61 cgttagaatt gaatcaccac aacacaatca aaggagcgtt ctgtatgcct gatcttcatc
      121 atatcaaact actacttgga atcaaagata aaaacatttt catcacaaat gtggaatcga
      181 agagcattaa gaatatcaag agtcttgttg tttctgcgac gttaagtaaa gaaattcgtc
      241 gctgtccttt gtgtaaacag atgaatcatg aaggcatgat cgttaagaac ggaaagaaaa
      301 aatcgctcat tcaacttaat aaatgtgcga accaactgac ttatcttgct ttagcgaaac
      361 aacgctacca ctgtcgtggc tgccacacct attttacggc gaatacctac attgtcgata
      421 gaaactgctt tatcgctaaa caagtgcgct ataaaatttt agaagagcta actgaaaaac
      481 aagcaatgac gacaattgct aagcattgcg gcgtttcttg gtcgaccgtt tctcggacac
      541 tcgcttctct gatccccatg acaaaagtaa aaagaaattg gttaccgcgt tgtttattaa
      601 tggatgaatt tcgttcactg aaaaatcaag tcggtcccta ctccttttct tgtatggatg
      661 gcgatactgg aaaattactg gatattcttc cttcacggaa gaaaaaggct ctcgtttctt
      721 attttatgca atttgaacga cgcgcacgat tgaaggtaaa ggtcatcgtg acggacatga
      781 atgtttctta tgcctctctg ataaaggaat gttttccaaa ggcaaaactc gttgtggatc
      841 gttttcacat tgtcaaacat ctcattcgta aatttgagga tattcgcgta cgaatcatga
      901 aaagctttga tcgaaacgat ccaacacaag ccaaatatta tcgacaatta aaggcgttgt
      961 ctcggcttct gatcaaacga caggacaagc ttgtttatga caaatggacc aaatggcgca
     1021 attttggttg ggcgtatctc accgaaagtg aagtggtgga tcgtctgctt tcgatctctg
     1081 acgaattacg aatcgcctat ttttactatc aagaaattct tcaagcgttt cacgacaaag
     1141 aggcggacac tttctttaaa cttgtaagaa cgatgcccaa ttccgttcca aaagaactgc
     1201 attctataaa aaaagctttt ataaactatg aatccggcat tcgtttagcc ctcgaattgc
     1261 cttattcaaa tgccaaaata gaaaatctgc atacccacat caaagcattg aaacgagtcg
     1321 cttatggctt tcgaagcttt cggaaaatga agaccagaat tttcttacta aataatctga
     1381 ttacctatga atcaaaaaac atctaaagta gaaaaggaat tctaccttag atgttcttat
//
LOCUS       DARBEC010000200         1424 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.209, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000200 DARBEC010000000
VERSION     DARBEC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.209"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..276)
                     /locus_tag="SCL80_002849"
     CDS             complement(<1..276)
                     /locus_tag="SCL80_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEG1108667.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSI"
     gene            401..1186
                     /locus_tag="SCL80_002850"
     CDS             401..1186
                     /locus_tag="SCL80_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
                     /protein_id="HEG1108668.1"
                     /translation="MPNKNRTFESILNEGLTTEPASIIFDEVIDDVEDSKYHKNLKEL
                     FYFALNNGPSLFTGISVPDSDNPLIMAKIYLTKWCNKYIRDRNNPALKKPLKTFGEKD
                     AALTTRVAANANIDDQKVLNDYLRGHFLYMSAENMNGSILEEYLAEVLEPEGWIWCAG
                     SVYRAVDFCYLGTSPILLQVKNKYNTESSSSSAIRVGTTIRKWNRLNKSTKISGLDSP
                     IPNWKALIEMTEASKELAAKLTENSYLAYINEKSTRELWTLDD"
BASE COUNT          533 a          232 c          218 g          441 t
ORIGIN      
        1 gatcgaatac cccaaaatat caacgattgt ggcaatcagg tcttcttttg attcttcatc
       61 tgaaagtaat tcacgataca acattgtttg tttgcttact gtgtcatatt cctctaagga
      121 ttcatctgct aaaagaacca cttgttccaa caaacggtcg gataaatatt tatagaaaat
      181 cagtcccaat aaataatttt tatattctga cgcatccatt ttactacgga gattatctgc
      241 cgcactaaat aatttttgat ttaattctgc acccaaaaag atacactcct atctcttatt
      301 taaagttgct atataccctc ttattttagc atactccctt ttagaaaaag caaaaaaaaa
      361 agaaaaaatc ttattcttga taacaagcta cgtgacgtat atgcctaaca aaaatagaac
      421 ttttgaatca atcttaaatg aaggtttaac cacagaaccg gcatccatta tttttgatga
      481 agttatcgat gacgttgaag atagtaagta tcataaaaat ttaaaagaac ttttttattt
      541 tgcactcaac aatggaccaa gtttgttcac tggtatatca gtacctgata gtgataatcc
      601 attaattatg gcaaaaattt acttaacaaa atggtgtaat aaatacatca gggataggaa
      661 taacccagca ttaaaaaaac ctcttaaaac atttggcgaa aaagatgctg ctttaactac
      721 tagagtagca gcaaatgcta atattgatga tcaaaaagta ttgaatgact acttgcgtgg
      781 acatttttta tatatgagcg ctgagaacat gaacggaagt attttagagg aatatttagc
      841 cgaagtgtta gaacctgaag gttggatttg gtgcgccggc tctgtatatc gagctgttga
      901 tttctgttat ttaggaacct caccaattct tttacaagtg aaaaataaat acaatacaga
      961 aagcagttcg agttcagcta taagagtagg aacaacaatt agaaaatgga atcgtttaaa
     1021 taagtcgaca aaaatttctg gtttggatag ccctatcccc aattggaaag cattaataga
     1081 aatgactgaa gctagtaagg aactggcagc taagttaact gaaaatagtt atttagctta
     1141 tattaatgaa aaatccacac gagaattatg gacactcgat gactaacaat atccttatta
     1201 gtatctatcc acgatacatg gaaaaaatta taaacaaaca aaaaaacttt gaatttagaa
     1261 atttctatct tacctcagat ccttataata tcttttggat ttacgaaaca atccccacca
     1321 aagctttaaa gtataaattg attgtaaaaa atccaattac taaacttaag aaaaatcaac
     1381 actacttttt aggagacgat gagtttgaac aattagttta aaag
//
LOCUS       DARBEC010000201         1424 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.210, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000201 DARBEC010000000
VERSION     DARBEC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.210"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(150..1337)
                     /locus_tag="SCL80_002851"
     CDS             complement(150..1337)
                     /locus_tag="SCL80_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEG1108669.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DARBEC010000202         1418 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.211, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000202 DARBEC010000000
VERSION     DARBEC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1418
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.211"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..625)
                     /locus_tag="SCL80_002852"
     CDS             complement(<1..625)
                     /locus_tag="SCL80_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEG1108670.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFD"
     gene            complement(784..1050)
                     /locus_tag="SCL80_002853"
     CDS             complement(784..1050)
                     /locus_tag="SCL80_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1108671.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(1040..1396)
                     /locus_tag="SCL80_002854"
     CDS             complement(1040..1396)
                     /locus_tag="SCL80_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1108672.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT          390 a          277 c          179 g          572 t
ORIGIN      
        1 gatcaaaaat atctaagttg tcatcaaaat ctttatcagt tccggtaact tttttgttaa
       61 ttactgaagg tgtcttgtaa gaaatagaat ctaaatcaaa gcgttttgat tctaaattat
      121 tttgtgcttt ctcaattaaa taattcactt tttttgtgag aaaccttctg atattgtctt
      181 tgctatatgt tccataaaaa cccttatctt ctaaagagtc cctgtacttc ttaacttcta
      241 tatactcttc agccaaaact tgtggaactg gttcatctaa gaaaaaaacc cctacctcgc
      301 ctttgctatt tttataaaag taatcaattt ctgctatagt tcccgatcta taattatcaa
      361 ctggagaacc caacttatgg ccaaaaatcg ctaataatat atcactattt tctacaattt
      421 gtttattaat aacactttgc ccagagctat tatttatata agtaggaatc gcattcgttt
      481 cccatcgaat tggcatcaaa attacttttg tattatctgt attttctata ttccatctat
      541 atatggtttc ttctacttca ttcctctttg ctgctatatc acctggtgat gctataaata
      601 cttttattag atttatggtt ttcatttatt gtacctttct ctttatgtct ctttaaagta
      661 atttttgtat aaaatttatt gacttttaca ctataggaca acttttatct atttagtaca
      721 aaaaggccca ctaaactaat tgattagtta ggagcctatt acaggtatat tttcaactcg
      781 cttttaagca aattctacta ccagcttttt gcctaatgcc ctagcgatat catcaagttt
      841 atcgaaagat acgttatccc ctctctcaat tcgagcaata gtagattgag ttgtatgtgc
      901 tttttgtgca agctccgctt gagttaggcc tgcttcctcc cgtgcatgat aaagagcaac
      961 cgcagtttcg agttttcttc cttcacggtc atatgcttct gcaaaatctt tgtctttttg
     1021 cttttcttca atcaattttt taatcatcat ttgtgaacca ctcctctcta agttcttttg
     1081 catgtttaag ttcacttatt ggtgttttct ccgttttttt agtaaatcca tgggtaatga
     1141 cataccttcc attgaccaca tggaaataga tagctctttg gatgtttgat cctaccttac
     1201 ttcttaattc gtacaaatta gtgtctaatt ttttcaccca tttcaaccgt tgagctacta
     1261 gcatcccatt cttttctgtt tcttctattg tacgtaaaag cttcgcacta tctttttttg
     1321 gtaatgtttc caaccattct aaaaattcat catgaccatt aggtctttta aatgtttcaa
     1381 aagatatttt ttccataccc aattatatca tatattga
//
LOCUS       DARBEC010000203         1375 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.213, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000203 DARBEC010000000
VERSION     DARBEC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1375)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1375)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1375
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.213"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            191..445
                     /locus_tag="SCL80_002855"
     CDS             191..445
                     /locus_tag="SCL80_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEG1108673.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDIIQAKQEDNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            446..763
                     /locus_tag="SCL80_002856"
     CDS             446..763
                     /locus_tag="SCL80_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEG1108674.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
BASE COUNT          493 a          196 c          266 g          420 t
ORIGIN      
        1 gatgttaacg gaagtgtatg gtgagtagtt cactcgtatt ttgatatcgt ttgtatatgt
       61 tttcagcgtt ctaagtggct tattttgtcc aataataata tggagtggaa tcccttaaga
      121 aacaccgttg gcaattgttg ttataacatg ttataattta ttcatcaaaa actaaaagga
      181 gtgtgataac atgggaaaag aactaaaggt tagaaagatc ggcaatagcg tgggagtgat
      241 ccttccaagc tctcttggat taaaaagcgg agacattatc caagccaaac aagaagacaa
      301 cttattcata ttagatacta ctcaaatagc aaaagaacac gatcgcaaat tgattgaaga
      361 aagtttccaa gactttgaga aaggtttaac agtttcagaa attgaaatgg taaaagcttt
      421 cggaaaatac ggctggagtg aatagatgaa agagtacacg ctagtttatt ctaaaagttt
      481 tcagaccagc cttgaaacga taatagaaga atgggaaaat gagcttgctc tttctccagc
      541 cagcataaaa agatttgtag gtgcgatcca gaaggcttta gaactaacca aaaattttcc
      601 aactatgcat gaggaagttt ccgaaatcta tggaatggat atcccaacat atcggatcgt
      661 gatcggcaaa agctatgcct tattttatcg gatcgaccat gaaaaaaaag agatacttgt
      721 aggacgtatt ttccaatcta aacaaatgaa actaggcttt taacaataat taggaaaaag
      781 tcgctcatcc tctacaggtg ggtgattaat gccgctcgat atggttgtta accgtcggtt
      841 actcgaagat tgatatataa gacttagtac ctttttattt tgctccatgt tatggccgaa
      901 ggtctaaaga tacaatcatt agtctagaga aaaattaaga accttaggaa ctacggggat
      961 cgcttggtga ataagagaca ctgctattag tgaaaaaata agatatcaag tacgctctat
     1021 tcccaaaaat ttcgtgactt tactcatgag aggttcaagt agtatatttt taatcaaaat
     1081 tgacgtaact tttaataaat actacctaaa taaggtagta tttatttttg tgttttaaaa
     1141 gaggaagtcc aatatatcta tgggaaactt cttaaatatt atgttggtac atgaaaatat
     1201 acttccctgc tagactattt acaacatttt actttgtacg tattcttgtt ttaaaaggaa
     1261 tgggataaaa tataggtaca ataactattt gatgctaaat ataattaatg gaggagagat
     1321 agtgaacata cttaatgaaa aaagatatgt atggtttgct ggtaaaaaat atatt
//
LOCUS       DARBEC010000204         1364 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.214, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000204 DARBEC010000000
VERSION     DARBEC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.214"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..>1364
                     /locus_tag="SCL80_002857"
     CDS             80..>1364
                     /locus_tag="SCL80_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEG1108675.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQLI"
BASE COUNT          465 a          263 c          257 g          379 t
ORIGIN      
        1 tttaggtatt aaaaaacata tcgttctcta gtatgattaa atcaccacaa aataaacaac
       61 tgagaggacg atacgctata tgaatgattc tatcaaaaaa atgctgagaa taacagagaa
      121 agatttgatg attacagagg tctcttacga gacccttcag aagaaaaaga cgttggtcgt
      181 cgatgctgtt ctctcgccta ctcctcgtgc ttgtagaagt tgtggttcta ctgtggtaga
      241 tggaaacggg aaagcaatta tagtgaaaaa tggaaaaaag gaaacgattg tccgttttga
      301 acaatacaat catatgcctt tggttatgcg cctaaaaaag caacgctata cctgtaaaaa
      361 ctgtcgcacc cattggacgg ctcaaagtta ttttgtccaa cccagacatt caatcgcaaa
      421 tcatgttaga tataaaattg cttctttact gactgaaaaa gtatctttat cttttattgc
      481 gaaaagctgt caggtatctt tgaccaccgt tattcgtaca ttgaaagagt ttaaaagcta
      541 tttaccaaag caatctaaga agatcctccc cagagtattg atggttgacg aatttcgttc
      601 gcatgcttcc atagaagata agatgagctt tatttgcgcg gatggcgaaa caggaaaatt
      661 aatagatgtt ttgcctacgc gtaaattacc tcgattaaca agctatttct taggttgtac
      721 caatccagaa gaagtagaat tcttggtgac agacatgaac gccgcctact tccagctcac
      781 caaacgtgtt ctgccaaatg cgaaagtcgt gattgatcgg tttcatattg tcaaacacat
      841 gaatcaagcg ttcaatgagt tgcgtatccg tgaaatgaat gaacttcgta aagccggaca
      901 gaaaagccag gcagaaaaac tgaaaaagaa ctggcgcttt ttgctaaaaa atcgtgcaaa
      961 catcaaccat tatgaataca aaacatggaa aagtttccga gcaccaaaat acccatttct
     1021 tactgaagca atgatgattg atcgattgct tgagttttct ccgcccctaa aggaggcgta
     1081 tccctttttt catgaattag ttgaagcctt tcgagacaaa gaccctgact tatttttctc
     1141 cttattggca gaacttcccg aaacgttaga tgacagcttt cgggaaaagc ttcaaaacct
     1201 tctgacctat gaagaaggca tcaccaacgc aatgatctat ccttattcca atggaaaaat
     1261 agaagcgaag aatacccaca taaagacaat gaaacgagta tcctacggat ttaaatcatt
     1321 tgagaacatg agaattagaa tctttttgat caatcaatta atca
//
LOCUS       DARBEC010000205         1355 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.215, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000205 DARBEC010000000
VERSION     DARBEC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.215"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1355
                     /locus_tag="SCL80_002858"
     rRNA            <1..1355
                     /locus_tag="SCL80_002858"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DARBEC010000206         1351 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.216, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000206 DARBEC010000000
VERSION     DARBEC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1351
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.216"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            550..1083
                     /locus_tag="SCL80_002859"
     CDS             550..1083
                     /locus_tag="SCL80_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEG1108676.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          430 a          249 c          254 g          418 t
ORIGIN      
        1 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta ttaagaggtt
       61 gtgaaaaagc tgcactgcat caagtaataa gaacagccaa acaaaaatag ttcctcatat
      121 ttttcgtttg gttggactta ttaccgcaga tgcaagcttt tgaacaccgt ttattgagaa
      181 gttgtgaaat cagcgtcctg acctgagtat tagagaaaca aatggcaaaa tagttcctca
      241 tattttgcta ttttttgagc taataccgag ggtcagctga ttgaacaccg tttagtggag
      301 aagttgtgaa aaagtcgcct actttcgagt cgtaagaaat ttttttgaaa atggttcctc
      361 acaactttgc ataaatttga cttaataccg agaagtcatg atccccatct aactccaaat
      421 cacaaggaat gagttcaaaa actagctttt ctcaacaaat attacataaa aaattctatg
      481 ttttttgtta aatttatttg cttttttcaa aagtatgtta cagtaatttc ccaaggaggt
      541 tttacagaaa tgaattttgt tgccatggat tttgaaactg ccaatcatca gccttatagt
      601 gcttgttctc ttgcgctagt catggtaaaa aatagccaga tcgttgatga attctataca
      661 ttgatacagc ctgagactcc ttttttctgg cgaaatgtcc aaattcacgg aatccatcaa
      721 gaagacgtac gcaatgcgcc gaaattccca gaagtctggc aaactatcca accatatttt
      781 caaaaaaatc gtttagtcgt tgcacataat gctgcttttg atacaaaggt cttagcggga
      841 tgcttagatt attatcatct tgaacagcct aattatcttt ctttatgtac agtgaaaagt
      901 agccgtcgcc tgtttcctga atttccaaat catcgattga acactgtgtg cgaaaatttg
      961 aacatccaat tagatcatca ccatgatgca ttggaagata gtcgtgcatg tgcggaaatc
     1021 ctgctttatc aagaaaaaaa attcgggaca gatccattga aaaaacttgt gactataaag
     1081 taaaagaata cttatgaaac acacaaagag gtcgtgagcc tgccgtttca ctccgagtca
     1141 caaggagcaa tttcaaaaaa tagcttctca tgtttattaa atattgtgac tggtgaccga
     1201 ggagctggca ggagaacacc gtttattgga gaggttgtga aaaagctgtt ctgcatcaag
     1261 taataagaac agccaaacaa aaatagcttc tcatattttt catttggttg gacttattac
     1321 cgcagatgca agcttttgga caccgtttac c
//
LOCUS       DARBEC010000207         1340 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.217, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000207 DARBEC010000000
VERSION     DARBEC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1340
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.217"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            39..656
                     /locus_tag="SCL80_002860"
     CDS             39..656
                     /locus_tag="SCL80_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108677.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMTKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
BASE COUNT          501 a          175 c          212 g          452 t
ORIGIN      
        1 gcgtttgatt ttcaaacttc gcaacagaac cgagaacctt gggttttcaa gtgctttttg
       61 gttcattaat cttaaccata gtgtttttaa agtggaaaac aagcaacaaa aaaagtatgt
      121 ggagccttgt aggaatagtt cttattattt ttactgtgac tgcagcaaca gaagcaaatt
      181 attcgcataa aattcagtcg tataaaggtc gtttagttat gaccaaaaaa gatgttacac
      241 gttatacatc ctcaagaact ggaggcctat atgactatgc accattagat gcattgaaat
      301 ataaagatca tttaaagaaa catgaagtaa aagttggtga tcggtgggag aatgcatctc
      361 ataaagcata taattccaaa atagtctata cagtagaaga tgccgatgag caaaatatca
      421 cattacctgt attcaattat tggggaacag ttgtgaaagt aaatgggaaa aagagcacta
      481 tagaaaatga caatggttta gtgtctatta aaggtacggg gaaaagaact atagttgagg
      541 tttcatctac gtatccaact acgatgattc tatctttatt aattagttca ataactttta
      601 tattcttatt tacacgttat ttaataagat gctgcggtga aatgaaaata gattagatag
      661 atattataaa tatgttgatt ctattttcat agcaatggag aaaatacatt agcaatatat
      721 aaaagaaggt atcaagagtc acagtgaatt tattccgaaa agtgaaaaga gcaaatataa
      781 aatttaatga aaatatagaa ctaaatagga aaacgtagac gtaattttct acgttttcct
      841 tttcatatat attaaatatt acggaaaatt atataaacta atttagtgtt ttgtttgtaa
      901 ttcctaattt tgctccactt ttagataatt tttaggtcta tttaactcat cagacattct
      961 atgctcattt ttctccaaat ttgtttcaac tctttcgtac ttaattttgc gtatttgaaa
     1021 attcttcttt tagagtactt ttattacgat actagtatga tatatatcta tagcaaacag
     1081 ttaaaatctt cctaaaataa gcgaatagca ttcctaaaac ctataaaatt tttagatgaa
     1141 gcgacaagtg gacaattaat aaactacttg aacatgaaat tatggatcac ctttcaaatt
     1201 tagaaaatgg aattcttata cttattgcgc aacatttatt gattgcaaaa ttatgtgaca
     1261 aaaaaaacta gtagaagaaa gaataatggc ttgataataa agtactacct attaaaggta
     1321 gtactttatt taatttagta
//
LOCUS       DARBEC010000208         1321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.218, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000208 DARBEC010000000
VERSION     DARBEC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.218"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            67..1074
                     /locus_tag="SCL80_002861"
     CDS             67..1074
                     /locus_tag="SCL80_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEG1108678.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            1086..>1321
                     /locus_tag="SCL80_002862"
     CDS             1086..>1321
                     /locus_tag="SCL80_002862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HEG1108679.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELS"
BASE COUNT          524 a          166 c          247 g          384 t
ORIGIN      
        1 gttatatatt ttttaaagat taagaatcga attatagatg actataattc ataggaggag
       61 atataagtgt ttaaaggaaa aacaatatta gtaactggcg gaactggttc cattggaagt
      121 gaaattgttc gtcagctttt aaaatatgaa ccaaaagcta tcagaattta tagtagaggt
      181 gaagaaaaac aattttatat gcaacaagag ctctcaaatc acactaattt gagattttta
      241 attggtgata tcagagactc tcaaaggatt gattatgcat gtcgtgatgt ggatattatt
      301 tttcatgctg ccgcaatgaa acacgttcca gcaagtgaat ataatcctat ggaagctgta
      361 aaaacaaatg tgatgggaac tcaaaatgta atagatgcag cgatcaaaaa taatgtaaaa
      421 cattttgtag gtatcagtac agataaagta gtgagtccta ctaacaccat gggtgcgaca
      481 aagttattga gtgaaaaatt aataatagct gccaatcagt ataaaggaaa aacagaaaca
      541 atattttctt gtgtaaggtt tggaaatgtc atggggtcaa gtggatctgt tattcctctt
      601 tttagtaaac aaataaaaaa caataaagat attactttga cagatgttga tatgacacga
      661 tttatgatgt caataccgga tgctgcaaga ttagttctgt ctgctgctat gaattcaaaa
      721 tttggagaaa catatgtctt aaaaatgccg gcaatcaata ttttaacttt agctcaatca
      781 ttaataaaaa tatataatga tacacatgat gaaaaatata gaggtgaaat aaaagttgta
      841 ggtatcaggc caggagaaaa attatatgaa gagctaatga ctacagacga aattgaacgc
      901 tcatatgaaa atgataaaat gtatatcatt ccaccacact atcatgctac tgacgaactg
      961 tacaaagatt atgaaaaaat taaaatcact gcatactctt cagatgcaac agaattaatg
     1021 agtggacagg aatttattag aatcgcaaaa gagaataatg tcgtgacttg gtaaggagtt
     1081 ttatgatgaa atcagaagta tatgtaattg cagaaattgg tgtaaaccat aatggaagtc
     1141 tagatttagc tttagaaagt attgataaag ctaaagaatg tggagctaac gcggttaaat
     1201 ttcaaacatt taaaacgaat aagctagttt ccaaaaaggc tccaatggca aaatatcaga
     1261 aggataatct taaagaggac agttctcaat ttgaaatgct aaaaaaatta gaattatctc
     1321 a
//
LOCUS       DARBEC010000209         1294 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.220, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000209 DARBEC010000000
VERSION     DARBEC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.220"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1244
                     /locus_tag="SCL80_002863"
     CDS             <1..1244
                     /locus_tag="SCL80_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEG1108680.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DARBEC010000210         1278 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.221, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000210 DARBEC010000000
VERSION     DARBEC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1278)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1278)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1278
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.221"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            172..1212
                     /locus_tag="SCL80_002864"
     CDS             172..1212
                     /locus_tag="SCL80_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEG1108681.1"
                     /translation="MTDFKFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCRVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFVPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAEIESGISIVFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNYE
                     SSPSTITKDWLE"
BASE COUNT          512 a          180 c          210 g          376 t
ORIGIN      
        1 agttcttaat gactttaaag gtagtacata attcatttta tttctgattt taggtagtat
       61 ttaattaaaa aacagtttta atgcgtttgc attttttatt tatctttgct attttcgaaa
      121 agagtaggta attttacata aataaaacaa cgtgaaagta gaggatctac aatgactgat
      181 tttaaatttt ttaaggcgga tagagtttac aacgaattat tttatcaatt tccaaaagtc
      241 tttattgttt ctgacgaata caaaaaaatg aaagattcaa ctaagattgc ctatatgctt
      301 ttaaaagcaa gattagagat cgcaatcagc aaacggcaaa tcgatgaaga aggtaatgtt
      361 tattttactt atacgacaaa tgaactatgt agagtattaa actgccaaaa acaaaaagcg
      421 atagcaatca aaaaagagtt ggaatccttt ggtttattat tacaaaagca gatgggattt
      481 aacaaacagt tagggaaaaa taatcctaat agactatatc tagcagaatt aaaagtctca
      541 gaaaatgata tctacttact cgaaaaattt gatagagaga atagggaaaa cgttgataaa
      601 tcagagggta tgaaaatcat acccaccctc gacgaaaaat cagacgctga atcccttggg
      661 gctcaagagg gtatgaaaat cataccgtgc caaaacgttg ataaatcaga gggtatgaaa
      721 atcataccag aacttaataa taatatatta gacactaata gacacaatat agacactgaa
      781 aaagaccgcc tacaagatca attgttgtta gacaattttg agacaattat gacaaacgac
      841 agcattgcta cgtttgtccc tgaacgagta ttaaatttga taaaaacatt ttcttcaagt
      901 tacagtgaag ctcaaaaaac cgtccagact attcataatg caaagaaaaa agctgaaata
      961 gaaagtggta tttcgatagt ttttgaagaa ctcgatagtt attatgtcaa tgcagaacaa
     1021 gaattataca cgacactgtt aaaagcctat caaaaattaa aaaccgaaaa agtcgaaaat
     1081 atccagaacc tgatttttgt ctatgtaaaa aattggttta tcgaaaaacc aatagctgct
     1141 aaagtatcaa gtgaaaaacg tttgaattat gaaagctccc caagcactat tacgaaagac
     1201 tggttagagt gaaactttac tgacaaagtg attgttgctt catggagtaa cattaaaatt
     1261 aaaaattttt tattattc
//
LOCUS       DARBEC010000211         1267 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.222, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000211 DARBEC010000000
VERSION     DARBEC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1267)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1267)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1267
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.222"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            12..>1267
                     /locus_tag="SCL80_002865"
     CDS             12..>1267
                     /locus_tag="SCL80_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEG1108682.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWSNLRQ
                     KIISNNQAELNDSPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIERENPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIIQIELK
                     QVTAKDGFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKTLMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGVSSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVV"
BASE COUNT          423 a          222 c          285 g          337 t
ORIGIN      
        1 agggaagaag aatgaaaatt agtcaccgtg aggaagcgga agtcgaagaa cagttgattc
       61 gtgtcctcgg ggaaggacat aatcagtgga cgtatcgtcc ggatctaaaa tcagaagaag
      121 atctttggag caatttacgt caaaaaatca tctcaaataa tcaagcagaa ctgaatgatt
      181 ctcctttgac cgataaagag tttgaaacga tcaaaaccga attattgcta cggacgaaaa
      241 ccccatttga tgctgcgaaa tggttaaaag gtgaaaatgg gatggctcgg atcacgattg
      301 aacgggaaaa tccgcaattg ggttctgtct cacttatcct ttattccaat caagacattg
      361 gtggaggaat ctctacttat gaagtggtcc atcaaattgc aaaacgtgga agtaacatag
      421 aagcgcggga tcgacgtttt gacgtcacct tattgatcaa tggcttaccg attatccaaa
      481 tcgagctaaa acaagtaact gcaaaagatg gtttttatca ggcgtttaat caaatcaaaa
      541 aatatgctga agaaggcatg tttcggaaca atatcttttc gaccttacag ttgtttgtgg
      601 tttccaatga acaaacgact cggtattttg caaatgcctt accgaaggat ttgcatccta
      661 agtttttatt tagttggcga acgaaagata ataaaaaggt agaaaactta tatgagtttt
      721 gcaaacaagt gctaaatatc ccagatgccc atcgcttgat tgcggattat acgattgtca
      781 gtgaagatca agacaataaa accctcatgg tgttacatcc ttatcaagtg catgcgattc
      841 aagccttgtt tattgccgca aataaacatc aatcagggta tgtgtggcat gcgactggtt
      901 caggaaagac gttgactagt tttgtatcta ctaagctttt agcacgaaaa tcggggatcg
      961 atcgaacgat catgttagtg gaccgcaaag atttagataa tcagaccacc acagagttta
     1021 cgaaatttgc ttcggagttt aatactgggg tttcttctgg taatgccaaa gcgaatagct
     1081 tgatcgtggg aacaggaagt gccaaagagt taagtgaaac tctgttggca gatgccaatg
     1141 cgaatgttgt gatcattaca acccgtcaaa aactagatgc ggcgttgaaa tatgcaaaga
     1201 aacaggaaga gaaaaaagga accaatcgct tccaaaaatt aatggggcaa catatcgtgt
     1261 ttgtagt
//
LOCUS       DARBEC010000212         1246 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.223, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000212 DARBEC010000000
VERSION     DARBEC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1246)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1246)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1246
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.223"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            200..742
                     /locus_tag="SCL80_002866"
     CDS             200..742
                     /locus_tag="SCL80_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108683.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..>1246
                     /locus_tag="SCL80_002867"
     CDS             898..>1246
                     /locus_tag="SCL80_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEG1108684.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMP"
BASE COUNT          514 a          141 c          238 g          353 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccag
//
LOCUS       DARBEC010000213         1215 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.224, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000213 DARBEC010000000
VERSION     DARBEC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.224"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..256
                     /locus_tag="SCL80_002868"
     CDS             <1..256
                     /locus_tag="SCL80_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108685.1"
                     /translation="FYIEDIAVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDN
                     NLIACKFYHNCGFKIGSVDTMLYANFENNFEKAVFWYLRF"
     gene            349..1143
                     /locus_tag="SCL80_002869"
                     /note="aph(3')-IIIa"
     CDS             349..1143
                     /locus_tag="SCL80_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1108686.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          385 a          206 c          296 g          328 t
ORIGIN      
        1 cttttatata gaagatatcg ccgtatgtaa ggatttcagg gggcaaggca taggcagcgc
       61 gcttatcaat atatctatag aatgggcaaa gcataaaaac ttgcatggac taatgcttga
      121 aacccaggac aataacctta tagcttgtaa attctatcat aattgtggtt tcaaaatcgg
      181 ctccgtcgat actatgttat acgccaactt tgaaaacaac tttgaaaaag ctgttttctg
      241 gtatttaagg ttttagaatg caaggaacag tgaattggag ttcgtcttgt tataattagc
      301 ttcttggggt atctttaaat actgtagaaa agaggaagga aataataaat ggctaaaatg
      361 agaatatcac cggaattgaa aaaactgatc gaaaaatacc gctgcgtaaa agatacggaa
      421 ggaatgtctc ctgctaaggt atataagctg gtgggagaaa atgaaaacct atatttaaaa
      481 atgacggaca gccggtataa agggaccacc tatgatgtgg aacgggaaaa ggacatgatg
      541 ctatggctgg aaggaaagct gcctgttcca aaggtcctgc actttgaacg gcatgatggc
      601 tggagcaatc tgctcatgag tgaggccgat ggcgtccttt gctcggaaga gtatgaagat
      661 gaacaaagcc ctgaaaagat tatcgagctg tatgcggagt gcatcaggct ctttcactcc
      721 atcgacatat cggattgtcc ctatacgaat agcttagaca gccgcttagc cgaattggat
      781 tacttactga ataacgatct ggccgatgtg gattgcgaaa actgggaaga agacactcca
      841 tttaaagatc cgcgcgagct gtatgatttt ttaaagacgg aaaagcccga agaggaactt
      901 gtcttttccc acggcgacct gggagacagc aacatctttg tgaaagatgg caaagtaagt
      961 ggctttattg atcttgggag aagcggcagg gcggacaagt ggtatgacat tgccttctgc
     1021 gtccggtcga tcagggagga tatcggggaa gaacagtatg tcgagctatt ttttgactta
     1081 ctggggatca agcctgattg ggagaaaata aaatattata ttttactgga tgaattgttt
     1141 tagtacctag atttagatgt ctaaaaagct ttaactacaa gctttttaga catctaatct
     1201 tttctgaagt acatc
//
LOCUS       DARBEC010000214         1189 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.226, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000214 DARBEC010000000
VERSION     DARBEC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.226"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            21..974
                     /locus_tag="SCL80_002870"
     CDS             21..974
                     /locus_tag="SCL80_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEG1108687.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            946..>1189
                     /locus_tag="SCL80_002871"
     CDS             946..>1189
                     /locus_tag="SCL80_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEG1108688.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEE"
BASE COUNT          425 a          187 c          229 g          348 t
ORIGIN      
        1 ataggaaatt ggagggaact atggaaaact tacggactgc atttaaaaca tcagataaac
       61 taggaaaagt tgtaatgctt acaggtagaa aaggcggcat tggtaagact acagacaatg
      121 atttattagc gattgtttct agtcaacttt ttgaaaaaga cgtgttgcta attgattatg
      181 accaacaaag aaatactact tccaacattg gatcaacgta tcaaattact agctttgatc
      241 gatccatgtc tgcagcaatc aaaaaagggg actgggtttc aggtattact caagtaagtc
      301 cacatttata tattatggct ggctctcctg gatctgaaga acttaatgaa tacttgtccg
      361 aaaaatatcc tgatcgtcgt aagagatcat tggccttcat taaaccttta gaagaattga
      421 gaaaaaattt cgattatatc tttatcgact gcccaccatc aacagataat gtggttcgtg
      481 cattcttaac tgctgcagat tatattattg caatgcaaga attaaaacgg tacgcaatgg
      541 aaggaacaga agattttatt aataaagttt tagttccgat tgtcacgaat tttgaagagt
      601 ctcatcttca aatcattgga attttgcctg ttcttttctc agtgcgacgt tcctctcaac
      661 acgcaaacta tcaaaaaaca attgataagt atggtgaatc aaacattttt aaaagcattg
      721 ttaaaggctc ggatcggtta gaaatgtatg gtgagaatgg tatccaatta aatgattatg
      781 tagatcgaag atggtgggct atttttgcag acatctttac agaattagaa gaaagaattg
      841 aatactatga acaacatggt gatattgaag gatttgatta tacacctaaa tatgctgact
      901 caatggccaa tcatatccta cctcttggaa aggagattaa actaaatggc attattacca
      961 acgaacaaag ataagaagaa aattggtgct gaagatattt caattaaaga tgatattaat
     1021 gtcgagttag gggaacgaaa gtacactgcg aaagaaagaa aaccagttca agtagatcct
     1081 cccgtactaa aaaccattcg ggatcttagt tatgcaaaag atattcctat gtatgaaata
     1141 gtcaaacttg caatggacgc ctatgttgat actttaaacg acgaggaac
//
LOCUS       DARBEC010000215         1177 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.227, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000215 DARBEC010000000
VERSION     DARBEC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1177)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1177)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1177
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.227"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..156
                     /locus_tag="SCL80_002872"
     CDS             <1..156
                     /locus_tag="SCL80_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEG1108689.1"
                     /translation="DKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPVDA
                     ISYGVRK"
     gene            175..>1177
                     /locus_tag="SCL80_002873"
     CDS             175..>1177
                     /locus_tag="SCL80_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEG1108690.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     "
BASE COUNT          500 a           95 c          205 g          377 t
ORIGIN      
        1 gataaggata agtgtattaa ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat
       61 tcagttgaga attgcaatca tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt
      121 ccagtggatg caattagtta tggagtaaga aaatagagag gagtagttag gaaagtgata
      181 aaaaatgcta tagcatatat tacaagaaag aaaaatagaa ctttaataat ttttatcata
      241 ttaactatag ttctttcttg cttgtattct tgtttaacaa taatgaaatc aagcaatgaa
      301 atagaaaagg ctttatatga aagttctaat tcttcaatat caataacaaa aaaagatggt
      361 aaatatttta atgttaatca atttaaagat attgaaaaat taaaagaaat tgaagaaaaa
      421 ataattcaat atgatggatt agcaaaacta aaagatgcta aagtagttag cggagaacaa
      481 agaataaata gagaagattt atctgacgaa tttaagaatg ttgtttcact cgaagctaca
      541 aataatacta aaagaaatat tttatttagt agtggagtat ttacaattaa agaaggtaaa
      601 aatatagaag aaaatgataa gaattcaatt attgttcatg aagaatttgc taaacaaaac
      661 aatctaaaat taggtgatga agttgatctt gaattactag atattgaaaa aagtggaaaa
      721 ataaaaagtc ataaatttaa aattataggg atcttttctg gtaaaaaaca ggaaacatat
      781 acaggattat cttctgattt tagtgaaaat atggtgtttg tagattattc aacaagccaa
      841 gagatattaa ataattcaga aaataatgaa attgcaaata aaattttaat gtattctggt
      901 agtgcagaat ctacagactt agccttaaac aaattgaaag agcttaaaat tgatgagtca
      961 aagtattttg ttgaaaaaga ttctaatgcg tttgaagagt ctttagagtc agtgagtgga
     1021 ataaaatata taataaaagt aatgacttat tctattatgt taggtgggat ggttgttctt
     1081 tcattaatct tgattctatg gttaagggaa agaatttatg aaataggtat attcttatct
     1141 attggaacat ctaagataca aattataatg caattta
//
LOCUS       DARBEC010000216         1166 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.228, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000216 DARBEC010000000
VERSION     DARBEC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.228"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(257..1129)
                     /locus_tag="SCL80_002874"
     CDS             complement(257..1129)
                     /locus_tag="SCL80_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEG1108691.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPKLAWQNFDFIGTMKKEFPIPIS
                     WTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGVGGGALQEGRFIEGFSHPEMGHMLVV
                     PHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGKKGQEVAEDDPYWEIEASYIAQCAYN
                     TTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKLVNGYVETPELSKYIVTPALEDNAGT
                     LGCLALAREAVLHS"
BASE COUNT          320 a          278 c          225 g          343 t
ORIGIN      
        1 atttgagtgt aacataaatg gcttttttat ttgtctcgct taattataca aacggcgtgt
       61 aaaaaactat tgcacaatac aagtggccta cggctatcga aatttccata actttcgagc
      121 aagaatttat ctcattcaag gcttgatctt ccaagtaaaa caaaaaccag ttaagcactc
      181 tgcctaactg gttatgacta aattttcagt tgtatctttc tctaccaaca ctaaatgtcg
      241 aagagccttt tttcgtttat gaatgtaaga ccgcttctcg agccaatgcc aaacaaccga
      301 gcgttccagc gttgtcttct aatgctgggg tcacgatata tttgcttaac tcaggtgttt
      361 cgacataccc attgaccaat tttgcaaatg cgtcgtggac gttttgcagc atatggcgtt
      421 gtttcatcac accaccgcca aaaatgatca catctggaga aaacatcaag gttgtattgt
      481 aggcacattg agcaatatag gaggcttcga tttcccaata aggatcatct tctgcaacct
      541 cttgtccttt tttccctaag cgtttttcga tggcgggtcc agcggccatc ccttctaaac
      601 agttgccatg aaatggacag ctgccagcaa agtcatcttt tggatgaggg acgacgagca
      661 tatgccccat ttctggatga ctaaaccctt cgataaagcg cccttcttgt aaggcaccgc
      721 cgccaacacc cgtaccgatc gtatagtaaa cgacactgct caatcctttg ccactgccga
      781 agacatactc accataggca gccgcattga catctgttgt ccaagaaatc gggatcggaa
      841 attctttttt catggtaccg atgaaatcaa aattttgcca tgccaatttc ggggtcgagg
      901 taatgtaccc ataagtggcg gaatcgcgat ggatatcgat aggcccaaat gagccaatcc
      961 caatccctgc taaatctgct tggtattgtt tgaagaagtc aatgactaaa ggcattgttt
     1021 cctcaggtgt cgttgtcgga aagctcactc tttccttgat cgtcatttct tcatcgccaa
     1081 tggcacacac aaattttgtg ccgcctgctt caattgcacc gtatagcata gacatttctc
     1141 cctttatttt agaatgaaat cactaa
//
LOCUS       DARBEC010000217         1164 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.229, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000217 DARBEC010000000
VERSION     DARBEC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1164)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1164)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1164
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.229"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..834)
                     /locus_tag="SCL80_002875"
     CDS             complement(<1..834)
                     /locus_tag="SCL80_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30-like element IS6770 family transposase"
                     /protein_id="HEG1108692.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTLYRLFSKGIFDIDTLPMKGKRKPNGHQEKRGKQQYQRSIHDRPDNYPHFNSEF
                     GHLEGDTIVGIHHKSAVITLVERLSKVIITIKPNGRKALDIETALNQWFSRFPKNFFK
                     SITFDCGKEFSNWKAISNQHDIDIYFADPGTPSQRPLNENSNGILRRNGLPKSMDFRK
                     VN"
BASE COUNT          360 a          202 c          203 g          399 t
ORIGIN      
        1 attcactttt ctaaaatcca ttgatttcgg cagtccatta cgacgcagaa tcccgttaga
       61 attctcgttt aatgggcgtt gagaaggtgt tccagggtcc gcaaaatata tatcaatatc
      121 atgttggtta ctaatggctt tccagttaga aaattctttt ccacagtcaa acgtaataga
      181 tttaaagaag tttttaggga agcgagaaaa ccattgatta agggcagttt caatatctaa
      241 tgccttacgg ccgttgggtt taatcgtgat aatgacttta gataatcttt caactaaagt
      301 aatgacggca cttttatgat gaatgccaac gatcgtatca ccttcaaggt gaccaaactc
      361 agaattgaaa tgaggataat tatcaggtcg atcatggatt gagcgctgat actgttgttt
      421 tccccgtttt tcctgatggc cattgggttt tcttttacct ttcatcggta gtgtgtcaat
      481 atcaaatatt cctttagaaa ataaacgata aagtgttctc ataccgcatg aaacaggcct
      541 ttcttttcgc ccgataatga cgtcaggcgt ccaaccaaga gtgactttct ctttaatgta
      601 atctacttca tgagcaggta attgaatgac ttttctacca cattttttct tattttcttt
      661 atactggtgc caataatcaa gagcagtctt gccttgcttg aacttattga ctacattata
      721 aatagtttgt atagcacgtc ccattcggtt agcgatttca accggcttat tatgttgaag
      781 ataatatgat tctatcattg tcagttcgtc tatggtaaga tgtttgtagg tcatttatgg
      841 ttacactcct ttgttttctt tcgtcggaaa tacaatttga gtgtaccata aatgcctttt
      901 tatttttcta acttaatttt acaattcgcg atatattaaa caacgttgtc gcgcttcatt
      961 gaaacatttt atgaaaattc caaaaaagag ttgacacatg tttttagacg tgataagata
     1021 actaagtcgt taaaaagaac cacttgcgaa gcttgattct atcgaaaaaa aacttcaaaa
     1081 aagttcttga caaataacaa acgataagtt atgataaatg agttgctgaa agatttcgaa
     1141 aaacgaaatc gaaaaaaact ttta
//
LOCUS       DARBEC010000218         1143 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.230, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000218 DARBEC010000000
VERSION     DARBEC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.230"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCL80_002876"
     CDS             complement(18..923)
                     /gene="xerC"
                     /locus_tag="SCL80_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEG1108693.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
BASE COUNT          338 a          232 c          194 g          379 t
ORIGIN      
        1 tgttcttttc cttattttta cgctcttgga tggaaagaac gatagttttt ttgtaaactt
       61 tcttttgtta catgggcata gatctgggta gtcgataaat tggcatgtcc tagcaattct
      121 tggactgtcc gcaaatctgc cccattattt aataaatgag tagcaaatgt atggcgcagc
      181 atgtgaggat ggatatcact atccaaactg ctttttttga tcatttgatt caagacatat
      241 tcaattccag taggcgtgat tggttttcca tgatgattca caaaaacgat atcatgctct
      301 tcgtgatatt ttgtcatcaa tacctggcgg ccttccctga aatattcttg cagagcatct
      361 tgagcaaaag aacctagagg agcatatctc tctttgttcc ctttaccatg gatcaacatg
      421 acctctgaat caaaatcaat gtctgatacc gacaaactgc tacactcact taaacggatg
      481 cctgacccgt aaaggacttc cagtaaagcg cggttcctca aatccaaagg actatctcct
      541 gctacgctgt caaaaagtgc ttgcatttca ttttcataaa aaaaacgtgg cagtctcaaa
      601 tttttctttt tgagatgaac gtaagaaaat ggattttctt ttatcacttc ttctttgaga
      661 agatactgat aaaacgaacg taggctggct atcttgcgac tgatagaatt tctgctatag
      721 ctaggacgat gatcagctaa tgcactcaaa tagacacgaa ccgtaaaata ttccgccttc
      781 agcagatcag cgtctccaga ttcctccaaa aaatgtttga aatggagaat gtcttcttca
      841 tatgcttctt tcgttttttc tgaatatcct ctttcgacaa tcaaataacg caaaaatttt
      901 tgttcccacg tggcttctgt cattttctca ccttcctttt gtcccagaga ataatttagc
      961 acagcgttta taaaaagaca aataaattgt cagaatttaa ccaatatttc taagtttgtt
     1021 cacaattggc gcaattcctc acattttgtt tgagaaattc ttaagaaaaa cgcaagaaaa
     1081 agggctctat aatttatggg actgatgaat cagaattgat tcttcagccc cattttcatt
     1141 tta
//
LOCUS       DARBEC010000219         1138 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.231, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000219 DARBEC010000000
VERSION     DARBEC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1138)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1138)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1138
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.231"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            54..464
                     /gene="spx"
                     /locus_tag="SCL80_002877"
     CDS             54..464
                     /gene="spx"
                     /locus_tag="SCL80_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1108694.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            478..834
                     /locus_tag="SCL80_002878"
     CDS             478..834
                     /locus_tag="SCL80_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEG1108695.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
BASE COUNT          403 a          164 c          216 g          355 t
ORIGIN      
        1 attttaaaag aaattactgt agaattggat tataattaaa aatgagtgag aaaatgataa
       61 aagtatatac ttcgcctagc tgtacttcct gtagaaaagc aagagcatgg ttgcaagcga
      121 atcaacttga atttgaagag aagaatattt ttgccgaacc gttaacagaa agtgagatca
      181 aaaagatctt ggcagcgact gaaggaggag ttcaagatat tatttccact cggtcgaaaa
      241 tttacgaaaa gctgggcata gattttaacg aattaacttt aaagcaaatg atcgtcttat
      301 tcaaagaata tccttcatta ttgagacgac ctattttgat tgatgaaaaa agaatactaa
      361 tcggatttaa tgaagatgag atccggtcat ttgtccctcg tgaaattcga tgtgttgcca
      421 gaaatctgat ctatttacaa aacaataaca tcttaaattc atagggaggg agccttcgtg
      481 gaagtgaaag tctatggatc agcgcactgt gtaacaagtc tgaaggcgaa acaatggtta
      541 gaacaccatc aattgcctta tcaatttgtt gacttagaag aacgcgatct aagtacagaa
      601 gaagcagaag aattatgcag ttttgaagaa gtgcaagcag aacagctatt tgcgacttgg
      661 tctgaggctt ttcacaacat tgcgattgac ctttcctgtg taggaaaaaa acaactgatt
      721 tttttatgca cacaacatac caatctcctc agacgtcctt tgattattat cgatgaagtt
      781 ctatttattg gatacaatga gcagttgatg gaacagctta tcttaaataa atagacttta
      841 actgttttaa tcaagtcact gagcatttca tacagttgta acagtcgaac acttagcatc
      901 aagcgtgtta tgtgttcttt ttttgacttt atttttctac tatagagaca tgctcaattt
      961 atgtggtcaa aattcaaatc taggtgggga tatagagtta aaaatatatt gacatgtgta
     1021 aatttacatg atatattcat ttctgttgtt ttaagtagca aagcaataaa atagtatttt
     1081 aaaaaacata taaaatagtt gttgacatgt tagaaatgac atgataagat aactgagt
//
LOCUS       DARBEC010000220         1129 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.232, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000220 DARBEC010000000
VERSION     DARBEC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1129)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.232"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            123..815
                     /locus_tag="SCL80_002879"
     CDS             123..815
                     /locus_tag="SCL80_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1108696.1"
                     /translation="MRKKIIINAGVIIGLMLVIFSGKQYADNILLWGGEDNIEKINNN
                     LTILDTALENKEQKISILNSQLSSNNQTLEQLRTNIEVSKQKVASLENDKNQLVSEKT
                     NLENQLSAKDSELQGKQNEINAKIDEINQKVAEINRLNSDWNNRLQNEKNKFNEEQST
                     INSLNQQINQMLSEKNNIQNQLQATQQKLEEEKSKNSSLNNYIEKLQKAKNDVEDTAN
                     KAQQIVNEHTIQ"
BASE COUNT          496 a          128 c          165 g          340 t
ORIGIN      
        1 atttctctat cctttttaaa taatttttta gacaaaaaac agaaagtgtc taaagaaagc
       61 ttgacgaaga aaataatttt taataactta tttaaggaaa ttttttgaag gagaagataa
      121 aagtgagaaa aaagattatt attaatgctg gagtaattat aggattgatg ttagtaattt
      181 tctccggaaa gcaatatgct gataatattt tattatgggg aggggaagat aatattgaaa
      241 agatcaacaa caatttaact atattagaca cagctctaga aaataaagag caaaaaatta
      301 gtattttaaa ttcacaattg tcttcaaaca accaaactct tgaacaacta agaactaata
      361 ttgaagtttc caaacaaaaa gtggcatctt tagaaaacga caagaatcaa ttagtatcag
      421 agaaaactaa tttagaaaat caattgtcag caaaagactc tgaattgcaa ggaaaacaaa
      481 atgaaattaa tgcgaaaata gacgaaatta accaaaaagt agctgaaata aatcgattaa
      541 atagcgattg gaataatcgt ttacaaaatg aaaagaacaa atttaatgaa gaacaatcta
      601 ctattaatag tttaaaccag caaattaatc aaatgttgag tgaaaaaaat aacattcaaa
      661 atcaactgca agctactcag caaaagctag aagaagaaaa gtcaaaaaat agtagtttga
      721 ataattacat tgaaaagctt caaaaagcta aaaatgatgt agaggatacc gcaaataaag
      781 cacaacaaat tgtaaatgaa catacaatcc aatagatata gttttaattt aacaaatggt
      841 atgtttggag ataatgagat agaatataaa tgtcataata ttatacaatc taaacaccgt
      901 ttccttttct atttttgatg acatcagtat atagctactc gaaagagtag tctttttatt
      961 attctcgctt attattgata tgaaaatttc gatttgacaa aaggtaccga ataattttgt
     1021 aatatgaatt attatcaaga agtaattatg atgataacat atggaaacta gttagtaata
     1081 ataacaggtg attaaacttt tgaacgactt gtaattctat attttttct
//
LOCUS       DARBEC010000221         1126 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.233, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000221 DARBEC010000000
VERSION     DARBEC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.233"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            122..>1126
                     /locus_tag="SCL80_002880"
     CDS             122..>1126
                     /locus_tag="SCL80_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEG1108697.1"
                     /translation="MSHTQLIKDTLNILDLNIYFEENCLTKEKYKGQICLIYKGTLLY
                     KPEECSHCLCVVPSRIIRWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADT
                     SVAEKHCFISQKVRWSVIARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQT
                     LPKVLCFDEFKSVRSVASAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREA
                     VQLIVIDMYAPYVSLVKKLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHS
                     QRGLGKKLKRYWKLLQKEEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQ
                     L"
BASE COUNT          340 a          217 c          222 g          347 t
ORIGIN      
        1 gctctatact ttttgatgtt ggtgaacttc accaacatct tttttgtata aaaaaatata
       61 gaaaagacac cctgaaatcc tttagaatta atttggctac caattcatag gaggattcaa
      121 gatgtctcat actcagctta tcaaagatac cctaaatatt ctagacctaa atatctattt
      181 cgaggaaaat tgtttaacta aagaaaaata caaagggcaa atttgcttga tttacaaagg
      241 aactcttctg tacaagccag aagagtgttc ccattgtctt tgtgtcgtcc cctctaggat
      301 cattcgttgg gggacgacaa ccgttcggtt gttactgaat gatgtgtctg agtatcgcac
      361 ttatctggag ctgaagaaac aacgatttaa atgtaagtct tgccaaagaa cattcgtagc
      421 ggatacctct gttgctgaaa aacactgttt cattagtcaa aaagttcgtt ggtctgtcat
      481 tgctcgctta aaagagaata cttccatgac ggaaatcgct cgtcagaaaa atatttccac
      541 ttcttcggtc tatcgtgtca tgaagcgatt ctaccgccca ttgaatcctt tcaaacagac
      601 tttgccaaag gtcttatgct ttgatgaatt caaatcggtt cgaagtgttg ccagtgcaat
      661 gagttttatc atgatggacg gacaaagcca tgcattattg gatattgtcg aaaatcgacg
      721 cttgccttat ttagaacgct atttttctcg gttttcatta gcgactcgtg aagccgttca
      781 attgattgtt attgatatgt atgcgcctta tgtttcgttg gtgaaaaagc ttttcccgaa
      841 tgctcagttg atcatcgatc gtttccacat cgtccaacat attggcagaa ctttcttgaa
      901 tcatcgagtg aaagaaacca cggtacgact taaaggaaat tcccactctc aacgagggtt
      961 agggaaaaaa ttgaagcgct attggaaact cctacaaaaa gaagaagcaa agttaaacta
     1021 tgaaaaacgg gtttggcgtg ccagttttaa agattatttg actgaaagtg agattgccga
     1081 tcgactgtta gctgcttgtc ccaacttacg ccaaggctat cagtta
//
LOCUS       DARBEC010000222         1117 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.234, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000222 DARBEC010000000
VERSION     DARBEC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1117)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.234"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCL80_002881"
     CDS             complement(<1..899)
                     /gene="vanY"
                     /locus_tag="SCL80_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1108698.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHT"
BASE COUNT          328 a          228 c          160 g          401 t
ORIGIN      
        1 ttagtatgtg ttgatccggg aaacactgtc acaattacac cgtctatatt gtttcctgat
       61 atctcataac gaagattagt cggcacatga atggtggtat ttttagtaac aggataataa
      121 aagatctcat atttttcccc atttacacta acagaaatgg ttttttcttc ttttaggtaa
      181 tccatatatt cctcgagaac gaaattcttt tctttcataa tcgcactatg tggtaaacca
      241 acatagcgaa tatgccatgg ttcatattga attcctgtta actctgtttt gtcctctgga
      301 taacgtaaaa tgaacccgta tttccaagca ttttcttcta tccactttcc ttcaggggct
      361 cgttccattt tcgtcaagct tgatcctaca tctagtgata aacctgaatt atgctcacta
      421 taacctgctg gtaaggcata ctcagccccc atttcttggt aaagcacact ttgctcatca
      481 aagtctcgat agccactatt aataataaaa tgactaacgc caccctttac agcatcattg
      541 accatctctg aaaatttttg tgctatttct tttgacatat aaatattact atcaagcaac
      601 ccgtatccat ttattaattc gtcatgttta gataaattca cgatatctga cttcacactt
      661 tcttggcgaa caggatattt actattgatt aatagcagat ttccttgata aacctgttct
      721 tctgtaattg ttttctcttg ggtattttca gaagtcccac tattttctaa atgttctttg
      781 ggattttgat cataattttg aaattcgact ttttcctgag aaaacagtgc ttcattaacg
      841 tagtcataac ctaagtatat taagaataac aataacaata aaaaaaacaa cttcttcatt
      901 ttcagtctcc tccttaacta tatagttgga agtatgaaat atttctagaa tcttcccctt
      961 aaacggcaca atctcgtttg agattgtgcc gttttgcccc gtaaattaca gtcaattatt
     1021 tcgccctact ggatttacta ttatcaccaa tgtagatgaa ttttcataag tgatacccat
     1081 gctgaattta acgaggctga actgcctgtt gacaatg
//
LOCUS       DARBEC010000223         1106 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.235, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000223 DARBEC010000000
VERSION     DARBEC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.235"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1087
                     /locus_tag="SCL80_002882"
     CDS             <1..1087
                     /locus_tag="SCL80_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEG1108699.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DARBEC010000224         1088 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.236, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000224 DARBEC010000000
VERSION     DARBEC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1088
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.236"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            456..1088
                     /locus_tag="SCL80_002883"
                     /pseudo
     CDS             456..1088
                     /locus_tag="SCL80_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          378 a          188 c          189 g          333 t
ORIGIN      
        1 atttttttaa aacactagat tactcactat ataaaatgtg attaatttta ttattaataa
       61 aatatttttt atattacaaa agtagtgttt caaagttagt agagaaaatg tagttatata
      121 tttaaatttt taccagaaag aaatgaacac cttcaattat ctaaatctcg aaaatggtta
      181 gatgtaatca tcaggaaaaa caatcttaaa cgaattacta ttcatggtct tagacacaca
      241 catgctagct tgcttttagc aagctagtgc aaatattaaa gatgtgcaag agcgtttagg
      301 ccactcttct attcaaatca ttatggatct atatattcat attacagaca aaagaaaaga
      361 agaaaccgta gcgcaattcg caaaatatag gctctataat ttatgggact gatgaatcaa
      421 aattgattct tcagtcccat tgttatttta ggtatatgat tcgtacattg aaagagttga
      481 aaaactatct tccgaagccg tcaaaaagga ccctgccaaa agtattgatg gtggatgaat
      541 ttcgttcgca cgcttcaatt gtggataaaa tgagctttat ttgtgcggat ggcgagactg
      601 gaaaactcat tgatattttg cctacacgaa aattgtctcg attaacacac tattttagtc
      661 gctgtgcaaa tcctgaagcg gtggaatatt tagtgacaga tatgaacgcc gcctacttcc
      721 aactgacaaa acgtgtctta ccaaatgcga aagtcgtcat tgattgtttt catatcatca
      781 aacatttgaa tcaggcgttc aatgattttc gtgtacaaga aatgaaaaga ttacaccagt
      841 tagggcaacg acgggaagcc gagaaagtga aaaagaactg gcgctttctt ctaaaaaacc
      901 gtcaaaaaat caatcactat gattacaaaa catggcgcag ttttcgtgca cctaaatacc
      961 cgctactcac tgaagcaatg atgattgatc gattgctggc attttcaacg cctttaaagg
     1021 acgcctatac ttttttccat gagctgaccg aagcctttag agacaaggaa tcagacttat
     1081 tttttacc
//
LOCUS       DARBEC010000225         1069 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.237, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000225 DARBEC010000000
VERSION     DARBEC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1069
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.237"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..969
                     /locus_tag="SCL80_002884"
     CDS             <1..969
                     /locus_tag="SCL80_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEG1108700.1"
                     /translation="PINTFAYTINDEYNLAPNQGDSRLAIPNKIILHETGIDAPARNV
                     AANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAGNANPYAPVQIELQRTYDKA
                     LFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIISHLWVTNYVWGNHTDPYGYL
                     SQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGAANPTLTDGTNHAHIDQFGE
                     IENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADGIYRPDVNQAYNTSGNVGYH
                     VSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRWYLNIPKR"
BASE COUNT          368 a          185 c          214 g          302 t
ORIGIN      
        1 cccattaata cgtttgctta tactattaat gacgagtata atttagcgcc gaatcaagga
       61 gactccagat tagcaattcc taacaaaatt attttgcacg aaactggaat agatgcacca
      121 gcaagaaacg tagccgccaa catgaaaaat aattataacg gaagcaattc ttatactaca
      181 gatgttattg gtgacggtgg gattgtttac cgtgtgggtg agcaaggata tgtttcgtgg
      241 ggagctggta acgctaatcc ttatgcgcct gtacagattg aattacagcg cacatatgat
      301 aaagcattgt ttgaaaaaaa ctatcgagct tatattgaat atacaagaga tagtgcaaaa
      361 aaatatggaa ttccattgac tcttgatcaa ggaacttctt tatttacaaa aggaatcatt
      421 tctcatttgt gggtgacaaa ttatgtttgg gggaaccaca cagatccata tggttactta
      481 tcgcaaatgg gagttagcaa agaaaagctt gcttatgatt tagctcatgg atttaccgat
      541 gaaaatccaa caacttctga aaacaagcct gtcattgatc caacacgagc tggtgcagct
      601 aatcctacgc tgacagatgg aacaaatcac gcccacattg atcagtttgg ggaaatcgaa
      661 aacgcaaact tgcatgtggc tggatggcac attgctaact ataaatacga gtatattttc
      721 attatggact acaatactgg gaaagaatta gctcgagtaa gagctgatgg aatttataga
      781 ccagatgtaa accaagctta taatacttct ggaaatgttg gttatcatgt atctttcaat
      841 atgcgtaatt ttcctagtaa gaaagtctat gtaatgatgc gggcaacgaa tgatccagag
      901 ggaaacacta aaggcggtgc gcaagatttc catgacaaac gttggtattt aaatattcct
      961 aaacgataaa aatagctcct cgttgaggag cagtacataa ctatattgac aactataaaa
     1021 attattcgat aaaatagtga tgttatcgca tatcttcact atcacccat
//
LOCUS       DARBEC010000226         1060 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.238, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000226 DARBEC010000000
VERSION     DARBEC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.238"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(38..994)
                     /locus_tag="SCL80_002885"
     CDS             complement(38..994)
                     /locus_tag="SCL80_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108701.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          344 a          176 c          160 g          380 t
ORIGIN      
        1 tttagaaaaa gcagtcggac tttctaaaag aagttcacta ttcgatcctg tcgatgattt
       61 cttcaggaaa atcgtctatt tgcttgtcaa ttttgctgtc tttgtatttc tcttcttgga
      121 tagtcaacaa aatctttgta agattccccc agtctactgc tcttggcgga tcgagccaaa
      181 ccactacttg agatgcactt tcttcatata gattattcct attcgtgatt ataacatccg
      241 ggaactcagg ctttcctgcc tctatcactt ccgtgttcag attaatgtac aagcttaaat
      301 ttttcttgat aaattgatta taggcagcac cataagaaaa atcgatgatg atctttactg
      361 gttcagaaat cagttgaatg ggtatgtgat tgatcaataa taataaataa ctgaaaaaaa
      421 gaaaaaattt atcttgaagc aactctttgc gttttgacgt tagatcattt atatactcta
      481 tcaaatagca ataaaatgct ggatagtttt ctctaaaaaa agaaacatca agtggaaatt
      541 catcagcttt attaatagtg taatgcaaaa gtttaaaatg tattttggtt aactctcttc
      601 ggatcaattg gctcgtctct tctcgcaaca gattaaactg attttcaaaa ctttcaatga
      661 atcgttcatt caaccattcg attgtttcat cttcattttt tctcaagtat ttttgatcca
      721 atttatcatg aagataaagc caagagagtg tcttattgat aaaaataacg gatttttctc
      781 ctaatactcc ttttaagtgg taaaatccta tatccgaaaa aaatagcgac tgattgctag
      841 caatatctaa agaatcagct tgttcagcca acaggttgat tgctaaaaag tggaataatt
      901 tctttttttc ataagcagac agaggataat actgttttaa atcttttaac attttttctg
      961 tttttataat tataggtagc gtatacaatt ccacaaaact tatctctgtt gagttaaaaa
     1021 gattatataa aaaaagccga atgtttttag gattccctgt
//
LOCUS       DARBEC010000227          997 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.239, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000227 DARBEC010000000
VERSION     DARBEC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 997)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 997)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..997
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.239"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(26..913)
                     /locus_tag="SCL80_002886"
     CDS             complement(26..913)
                     /locus_tag="SCL80_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEG1108702.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
BASE COUNT          288 a          207 c          179 g          323 t
ORIGIN      
        1 taatttcttt gcctttttat ctaaatcaaa cgtttagttc ttgaccaact aataaagaat
       61 aactggacat agacatgcca tttttcgcca ttagatcatc aacagacatg ccaaattttt
      121 gtgcaatatc ccatagcgta tcacctgaca cgactgtata tgtctgacca ttatttgtat
      181 ttacatctgt tgttgaagat gatgacgttg aagtcgtcgt agtagtcgtt gttgttgtac
      241 ttgtggttgt tgtagccgaa cctgttgatg gcgtatcaaa acgggttaaa ttataagtat
      301 taatcaatga gttcaatttg gccccataat ttggatcggt agcataacga cctgtcaagt
      361 acgctgttgc atcataaaaa cttgttgtat tgcttttcca cacgccagca taatgataat
      421 cgcccgtgga aaaactggtt gaacgaagaa cattcgcatg gtcttggaag gattcccaat
      481 aagaagaata ttttcggaat ggctcattca tcgttaccca ttgtccgttt acatattctt
      541 tggttgccat ataaacgacc tgtccgttat agcttccttt cacaccgaat agattataat
      601 ttggcgcact agctaatccg gaagtgccat atgcactttc caacaacgct tgagcgatca
      661 tgactgaagc atacaagtca ttacttgcag caacttctga cgcagaccag ccgatttgat
      721 cgataaacgc ttgaggatca gcgactgttt gagatccttg ttcatctgca tgaacatgtt
      781 gcggtaccgc tgtaaccatc atcggcccaa ctaaaagact cgttccaatt aaaggagcaa
      841 tcgctttttt attgatcacc atttttcttt taggtctatg gtgccgggca ctgcgggtct
      901 tcagctcttg cattcaaatt cctcctcaaa ataaaaaaac ttaataaaaa gttctcttct
      961 taaaacaaac ttaattaatt aaattatact tttaatt
//
LOCUS       DARBEC010000228          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.240, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000228 DARBEC010000000
VERSION     DARBEC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.240"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>994)
                     /locus_tag="SCL80_002887"
     rRNA            complement(<1..>994)
                     /locus_tag="SCL80_002887"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          209 a          307 c          225 g          253 t
ORIGIN      
        1 agcacgtgtg tagcccaggt cataaggggc atgatgattt gacgtcatcc ccaccttcct
       61 ccggtttgtc accggcagtc ttgctagagt gcccaactga atgatggcaa ctaacaataa
      121 gggttgcgct cgttgcggga cttaacccaa catctcacga cacgagctga cgacaaccat
      181 gcaccacctg tcactttgcc cccgaagggg aagctctatc tctagagtgg tcaaaggatg
      241 tcaagacctg gtaaggttct tcgcgttgct tcgaattaaa ccacatgctc caccgcttgt
      301 gcgggccccc gtcaattcct ttgagtttca accttgcggt cgtactcccc aggcggagtg
      361 cttaatgcgt tagctgcagc actgaagggc ggaaaccctc caacacttag cactcatcgt
      421 ttacggcgtg gactaccagg gtatctaatc ctgtttgctc cccacgcttt cgagcctcag
      481 cgtcagttac agaccagaga gccgccttcg ccactggtgt tcctccatat atctacgcat
      541 ttcaccgcta cacatggaat tccactctcc tcttctgcac tcaagtctcc cagtttccaa
      601 tgaccctccc cggttgagcc gggggctttc acatcagact taagaaaccg cctgcgctcg
      661 ctttacgccc aataaatccg gacaacgctt gccacctacg tattaccgcg gctgctggca
      721 cgtagttagc cgtggctttc tggttagata ccgtcaaggg atgaacagtt actctcatcc
      781 ttgttcttct ctaacaacag agttttacga tccgaaaacc ttcttcactc acgcggcgtt
      841 gctcggtcag actttcgtcc attgccgaag attccctact gctgcctccc gtaggagttt
      901 gggccgtgtc tcagtcccaa tgtggccgat caccctctca ggtcggctat gcatcgtggc
      961 cttggtgagc cgttacctca ccaactagct aatg
//
LOCUS       DARBEC010000229          994 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.241, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000229 DARBEC010000000
VERSION     DARBEC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 994)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..994
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.241"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..555
                     /locus_tag="SCL80_002888"
     CDS             <1..555
                     /locus_tag="SCL80_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108703.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVFRN
                     ISHKVRVNNRKEVCDDFKMVYQASSKEVALEARGAFAKKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEGELEQMLSQPMEN"
BASE COUNT          324 a          176 c          188 g          306 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttttcgta atatcagtca caaagtgcgt gtcaataatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tagaagcacg tggtgctttt
      241 gcgaaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataagca aatcaagaaa tacagccacc gcaaggaaca gttccaaaac
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcgagc tcgaacaaat gctgagtcaa
      541 ccgatggaga attagaaaca atctacagta gggaagaacc atttacacaa aattattgac
      601 gctcccaagc taaaactcct attaatcgat ctactaaaaa taaataaaaa aattcttttc
      661 aatttttatt tatggtatga cataaataaa gtaaagttat tcttctaggt taatcttatt
      721 aatactcttt ctcactagtg ttgcatgcaa cactagtgag aaagagtatt aaatatactg
      781 caaggcaaga catttggtaa ttgaagaata acaagcaaaa aaatagcagt caggtctctt
      841 gacaaagtat agttgctatt ctttttttag gccctacttt aatgaaattt ttgtttggag
      901 ggaacgactt cattcctttg attatttcag ttgaatttaa tagagaagcc tggcgtctat
      961 taagaaatac ttttcttgat gagcgtcaat tttt
//
LOCUS       DARBEC010000230          987 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.242, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000230 DARBEC010000000
VERSION     DARBEC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..987
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.242"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..327)
                     /locus_tag="SCL80_002889"
     CDS             complement(<1..327)
                     /locus_tag="SCL80_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEG1108704.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNI"
     gene            complement(327..>987)
                     /locus_tag="SCL80_002890"
     CDS             complement(327..>987)
                     /locus_tag="SCL80_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEG1108705.1"
                     /translation="KKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPD
                     WHPWEDYRNFFVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDS
                     FTLIIEHETGIVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTD
                     IQLYDEVMHDNNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEV
                     N"
BASE COUNT          334 a          164 c          109 g          380 t
ORIGIN      
        1 aatatttttg taatcataat tatattgagt aagatcaata ctctcagaaa aagcaggttt
       61 ttctaaaaat atgtaatcaa ctttttcaac aattacattt agtgtttcca gatgcttaaa
      121 agtcggattt gtaataaaag ctatatcata atgtgtatcc aaatcgtctc tattttgaat
      181 atacgttatt tcttcaaact ctttttttcc actgctacta taagctgtta tatcaaaatt
      241 aatagaattc ttttttccat agcttattag attttttaga tgtctctttc caattgaccc
      301 taacccaaaa aaaattactt tcatcattaa tttacctcaa tgtcgttaat taagttaata
      361 atatacttat ctttttcaaa agtatattgg ggttccattt tcttttccaa aagcgtgaaa
      421 aattctttaa tctcctctaa atatgcatct tcaataatag tatgtgaata attattatca
      481 tgcataacct catcgtacaa ttgaatatct gtcatactag aagtttcttc attccatgaa
      541 aatagagaat ccgggttacc ttcccattct attaaaatct tttcaccaat tatagttaaa
      601 tttcttttag ctactcttga aacaacatct acacttaaag ttccaactat accagtttca
      661 tgctcaataa taagagtgaa actgtccgaa taatcaatat caagattgga taatttctgg
      721 gtcattacag taaacgattt aactttccca aatgtatcta taatccaagg taattcaata
      781 gcgaatattt ctctacaccc attagttttt ttgtttgaaa caaaaaaatt gcgataatct
      841 tcccaaggat gccagtcggg taaatactgt cctacatggt attgataaga aattttttta
      901 ttatgcgtca ctttagactt aatatattga atttcttttc tcttcaaaaa tgtagaagat
      961 agataaagta atctatcttt cttttta
//
LOCUS       DARBEC010000231          986 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.243, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000231 DARBEC010000000
VERSION     DARBEC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.243"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..704
                     /locus_tag="SCL80_002891"
     CDS             <1..704
                     /locus_tag="SCL80_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEG1108706.1"
                     /translation="YSVIFMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAP
                     TESAYIWKEVLQDLRKRGLEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIA
                     HKVRVRGRKEICEDFKLVYQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFY
                     NFPHAIRRTIYSTNLIEGFNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRI
                     HKGFKEIQDTLESMI"
BASE COUNT          333 a          161 c          163 g          329 t
ORIGIN      
        1 attattccgt tatatttatg gatgcgacgc atatccctgt gaagcgacag actgtttcga
       61 aggaagcagt gtatattacg attggtattc gtttggatgg aaccaaagaa gttcttgggt
      121 ttactattgc cccaactgag tctgcttata tttggaaaga agttcttcaa gatcttagaa
      181 aacgtgggtt agaagaagtt ttattagtag tgacagacgg attaagctgt attgaagaaa
      241 gtatccatag tgtgtatccg aatgcccaat ttcaacaatg ttgtgtgcat gtatctagaa
      301 atatcgctca taaagttcgt gttcgaggtc gaaaagaaat ttgtgaggat ttcaaattgg
      361 tttaccaagc gaattcaaaa gaagaggcat tggatcacat cgactttatg actaggaaat
      421 ggaaaaagca gtatccaaga gtcgtcaatt tactcttgaa tcctgcccta ttaacctttt
      481 ataatttccc tcacgccatc agacgaacaa tttattcgac gaacctgatt gaaggcttta
      541 ataagcagct aaaacgatat actcgaagaa aagaacaatt ccctaatgaa gaatctctag
      601 agagattctt catttctcaa tttaatcaat ataaccaaaa atttttaggt agaattcaca
      661 agggatttaa agaaattcag gatacattag agtcgatgat ttaactgtaa ttaacagaat
      721 ggattttcca tttacatata attcttgacg ctaccataat ctaaataacc atcatattca
      781 tctactactt atataattga gttttattaa actttttaag tttctattct atcaaatatt
      841 taaacaatct ctgcatccga aaagactacc tcgtatagtt ttactaacaa tatttttatt
      901 agcgtgttca aatcctgatt tcttgtatca attattttct gttcactttc aataagaaaa
      961 aagtctttac ttccatggat aataat
//
LOCUS       DARBEC010000232          973 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.244, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000232 DARBEC010000000
VERSION     DARBEC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 973)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 973)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..973
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.244"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            6..>973
                     /gene="bsh"
                     /locus_tag="SCL80_002892"
     CDS             6..>973
                     /gene="bsh"
                     /locus_tag="SCL80_002892"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEG1108707.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKY"
BASE COUNT          344 a          128 c          172 g          329 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tat
//
LOCUS       DARBEC010000233          951 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.245, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000233 DARBEC010000000
VERSION     DARBEC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 951)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 951)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..951
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.245"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(107..511)
                     /locus_tag="SCL80_002893"
                     /pseudo
     CDS             complement(107..511)
                     /locus_tag="SCL80_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
     gene            complement(570..>951)
                     /locus_tag="SCL80_002894"
     CDS             complement(570..>951)
                     /locus_tag="SCL80_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108708.1"
                     /translation="KEICDDFKAVYQANSKEEANTFLSGMIEKWKKNYPKVTQSLIEN
                     QDLLTFYDFPPSIRRTIYSTNLIESFNKQIKRYSRRKEQFQNEESLERFLVSIFDTYN
                     QKFLNRSHKGFQQVTDTLVSMFTE"
BASE COUNT          287 a          168 c          157 g          339 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttccattggg agcgtcaata attttgtgta
      541 aataaattgt cctcctgcaa aataattagt tactcagtaa acattgaaac taatgtatcg
      601 gttacctgtt gaaaaccttt atggcttctg tttagaaatt tttgattgta tgtatcaaaa
      661 atgctgacta gaaagcgttc tagtgattct tcattttgaa actgctcttt tctacggctg
      721 tatcttttaa tttgcttatt gaaagactcg attagattgg ttgagtaaat ggttctacga
      781 atgctaggtg gaaaatcata aaaagttaat aagtcttggt tttctatgag tgactgcgtc
      841 actttaggat agtttttctt ccatttctca atcatgccgg ataagaaggt attcgcttct
      901 tcttttgagt tagcttgata aacagcctta aagtcatcac agatttcttt t
//
LOCUS       DARBEC010000234          948 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.246, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000234 DARBEC010000000
VERSION     DARBEC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 948)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..948
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.246"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..948
                     /locus_tag="SCL80_002895"
                     /pseudo
     CDS             1..948
                     /locus_tag="SCL80_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558187.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS1485 family transposase"
BASE COUNT          351 a          162 c          180 g          255 t
ORIGIN      
        1 gtaaagcaat ctaaaacgag tggttcattc aaagaaaaag ataatcttac gcctgaacaa
       61 aaagaattgt tagaactacg taaaagaaac cagcaattag aaatggaaaa tgatatttta
      121 aagcaagcag cgctgatatt cggacgaaga gacaagtaat cgatgcgaat aagcatcttt
      181 accctatatc agcgatgtgc agaatattag gtctatcacg tcagtcctat tattatcaat
      241 caaaaccaaa gaaagacgaa tcagaacttg aagaagtagt cgctgaagaa tttatccgca
      301 gccgaaaggc ctacggctca agaaaaataa aaaaagcctt atcaaaacga ggcattcaga
      361 tcagccgacg aaaaattagt agaatcatga aaaatagagg attaaaatcg agctatactg
      421 ttgcttattt taaagtacat cattctactt gcaatgaagc caaaacgaca aacgtattga
      481 atcgtaaatt cttaagagac aacccattag aagcgatcgt aacagacttg acttatgtac
      541 gagtcgggaa aaaatggaat tatgtctgtt tcattttgga tctgttcaat cgagaaattc
      601 tcggctattc ttgtggagaa cataaagatg ccgttctagt aaaaaaagca tttagccgta
      661 tcaaacaacc tctgacagag gttgagattt ttcatactga tcgtggaaaa gagtttgata
      721 accaagctat tgatgaatta ttaacaactt ttgacatcaa tcgatcattg agtcataaag
      781 gctgtccttt tgataatgcc gtagctgaat caacttataa gtcgttgaaa gtagaatttg
      841 tctatcaata cacatttgaa accttacaac aattggattt ggagttattt gactatgtca
      901 attggtggaa ccaccttcgg ttgcacggta cacttggcta cgagacac
//
LOCUS       DARBEC010000235          927 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.247, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000235 DARBEC010000000
VERSION     DARBEC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 927)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..927
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.247"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..788
                     /locus_tag="SCL80_002896"
     CDS             <1..788
                     /locus_tag="SCL80_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108709.1"
                     /translation="ALIDSKFIKVPKLKSLVRIKLHRQPKGIIKSATISRHSSGKYYI
                     SLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFTSKMEKKLKREQRKLSR
                     RALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNKLSTEMIKNHDIICIED
                     LNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKVDKWFPSSQICSECGHK
                     DGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          341 a          146 c          168 g          272 t
ORIGIN      
        1 ttgctttgat tgatagtaaa ttcatcaaag ttcctaaatt gaagtcatta gtaagaatta
       61 agcttcatag acaaccaaaa gggattatca aatccgctac aatatcacgt cattctagcg
      121 gtaaatacta tatttctttg ttgtgtaaag aagaaattat cgaattacct aaaactaatt
      181 ccacaattgg tattgactta ggtattatgg actttgcgat tctttctgat ggacaaaaaa
      241 ttgataataa taaattcacg tccaaaatgg aaaagaaact aaagcgtgaa caacgcaaat
      301 tgtctaggcg tgctttattg gctaaaaata aaggtaccaa tctatttgaa gctaaaaatt
      361 atcaaaaaca aaaacgcaaa gtagctagat tgcatgaaaa agtaatgaat caacgtactg
      421 attttctgaa taagttgagt acagaaatga tcaaaaatca cgatattatc tgtattgaag
      481 acttaaacac aaaaggtatg ttgcgaaatc ataaattagc taaaagtatt tctgatgttt
      541 cttggtcaaa gtttgtgact aaattacaat acaaggctga ctggtatgga cgaaaaatca
      601 tcaaagtaga taaatggttt ccgtctagtc agatttgttc agagtgcgga cataaagatg
      661 gcaagaaatc tcttgaaatt cgagaatgga cttgtcctgt ttgtcatact catcatgatc
      721 gtgatatcaa tgctagtatc aatattctga ccgaaggtct aagactacat tctatgggat
      781 tggcttagat ccaatcatca agaaccgtag gaactacggg gatagcttgg taaataagag
      841 acaccgctgt tagtaaagaa atacgctatc aagtatgctc tattcccaag aagctcccac
      901 ttcaagcgtt aagaaccgtc aggttta
//
LOCUS       DARBEC010000236          917 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.248, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000236 DARBEC010000000
VERSION     DARBEC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 917)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..917
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.248"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>917
                     /locus_tag="SCL80_002897"
     CDS             <1..>917
                     /locus_tag="SCL80_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="polysaccharide biosynthesis C-terminal
                     domain-containing protein"
                     /protein_id="HEG1108710.1"
                     /translation="QFLIQFAKGLEKVKAMGIAGIISTIVTIIANIFFLLILKWGLIG
                     FFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPLIATVVGWWINST
                     ADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKEYGDNETAKFYGN
                     TFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIANVLNCASGLLGPI
                     LSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSYVIYVVRKRAVGK
                     DIFIKHYHIVVESWGLLFVQAIIEIYFKN"
BASE COUNT          291 a          115 c          176 g          335 t
ORIGIN      
        1 tcaatttcta atacaatttg ctaaagggtt agaaaaagtt aaagctatgg gcatagcagg
       61 tattatcagc acaattgtaa caataattgc caatattttc ttcttgctta ttctgaaatg
      121 gggattaatt ggttttttca ttgcaaatat tttatcacag attatttcag ctacttatct
      181 gtcagtatta gtgagaatat gggaatatat cgattttgag gctaaagatc ctcagctaaa
      241 gagagaaatg ctaaattact cgattccatt aattgcaact gttgttggat ggtggattaa
      301 tagtacagct gataggtatg tggtttcctt tatagtaggt gtctcggcga atggcttatt
      361 gtctgtggca tataaaatac ctcaaataat taatactttg cagggaatat ttatacaggc
      421 ctggcaaatt tctgcaatta aagaatatgg agataatgag actgcaaaat tttatggaaa
      481 tacatttgtt atgattaatt tgttaatgtg cataacctgt tcaagtctta tcatattaac
      541 tagaccgttt tcacatattc tttatgcgaa agacttttat gaagcgtgga aatatgttcc
      601 atttttatta atagctaatg ttttaaattg tgcatcaggt ctgttaggac ctatcttatc
      661 agcaaaaaaa gattctaaat ccatgatgtg gtctgcaatc ataggggcat tagttaatgt
      721 gattatgagt gtagtccttg tttataagat aggggttcaa ggtgccacaa tagccactgt
      781 tatatgttca tatgtaatat atgttgttcg taaacgagct gtgggaaaag atatttttat
      841 aaagcattat catattgttg tggaatcttg gggattattg tttgttcagg caatcataga
      901 aatatatttt aaaaatt
//
LOCUS       DARBEC010000237          902 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.249, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000237 DARBEC010000000
VERSION     DARBEC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 902)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 902)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..902
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.249"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            263..886
                     /locus_tag="SCL80_002898"
     CDS             263..886
                     /locus_tag="SCL80_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEG1108711.1"
                     /translation="MYLAYLRVSSDDQSLARQHKLIENWSVKNNIAEDDLKIFEEKVS
                     GKNIENRPELQELISFIRERDTIIIPSLDRLARNSKDTKDLLQLFRSKGASIEILDLP
                     SFNGVTDPALRDLLTNLVIEVFSYVAENERKKIKERQKQGIEIAKKNGRYTGKKIQYH
                     ANATGKNKLIYDEIVDGLKKKETISKIAKNAGVTRKTVYRVKRDLGM"
BASE COUNT          332 a          129 c          167 g          274 t
ORIGIN      
        1 gttttaaaat aaactaatgt ccttattttt gtgttcacat ttctgttacc taagaaatac
       61 ttaaaggagg tgatgtagcc agcaacgact acaacttgaa cccgctatct acagtttatt
      121 tgttgtttct tttgggtata ctctaatggt acaaatccat atttgcttaa agcgttgatt
      181 tttcagcggt ttttctttat tgcaaagtgt accctaagac ctgtatacta atagtaaata
      241 gattggatag gaatgatggc aaatgtattt agcttattta agagtttctt ccgacgatca
      301 aagtttagct cggcaacata agctaattga aaattggtca gtcaaaaata atattgctga
      361 agacgattta aaaattttcg aggaaaaagt ctctggaaaa aatattgaaa acagaccaga
      421 gctgcaagag cttatttctt tcatacgtga acgagatacg attattattc ctagtcttga
      481 tcgattagca agaaatagta aagatacaaa agacttactt caattgtttc gtagtaaagg
      541 tgcttctatt gaaatattag acctaccgtc gtttaatggg gtaactgacc ctgcattaag
      601 agacttgcta acaaatttag tcatagaagt gtttagctat gttgctgaaa acgaacggaa
      661 aaaaatcaaa gagcgtcaaa aacaaggcat tgaaattgca aagaaaaatg gtcgttatac
      721 tggtaaaaag attcaatacc atgccaatgc aacaggaaaa aacaaattga tttacgatga
      781 aattgttgat ggtctgaaga aaaaagaaac tatttctaaa atagcaaaga acgcaggagt
      841 tacaagaaaa accgtttatc gagtaaaaag agatttagga atgtaatatg ttccagttag
      901 aa
//
LOCUS       DARBEC010000238          862 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.250, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000238 DARBEC010000000
VERSION     DARBEC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.250"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(49..121)
                     /locus_tag="SCL80_002899"
     tRNA            complement(49..121)
                     /locus_tag="SCL80_002899"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(86..88),aa:His,seq:gtg)
     gene            complement(124..197)
                     /locus_tag="SCL80_002900"
     tRNA            complement(124..197)
                     /locus_tag="SCL80_002900"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(163..165),aa:Trp,seq:cca)
     gene            complement(204..284)
                     /locus_tag="SCL80_002901"
     tRNA            complement(204..284)
                     /locus_tag="SCL80_002901"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(248..250),aa:Tyr,seq:gta)
     gene            complement(286..361)
                     /locus_tag="SCL80_002902"
     tRNA            complement(286..361)
                     /locus_tag="SCL80_002902"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(326..328),aa:Phe,seq:gaa)
     gene            complement(397..469)
                     /locus_tag="SCL80_002903"
     tRNA            complement(397..469)
                     /locus_tag="SCL80_002903"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(434..436),aa:Asp,seq:gtc)
     gene            complement(479..551)
                     /locus_tag="SCL80_002904"
     tRNA            complement(479..551)
                     /locus_tag="SCL80_002904"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(516..518),aa:Val,seq:tac)
     gene            complement(567..638)
                     /locus_tag="SCL80_002905"
     tRNA            complement(567..638)
                     /locus_tag="SCL80_002905"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(603..605),aa:Glu,seq:ttc)
     gene            complement(653..742)
                     /locus_tag="SCL80_002906"
     tRNA            complement(653..742)
                     /locus_tag="SCL80_002906"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(706..708),aa:Ser,seq:gga)
     gene            complement(782..855)
                     /locus_tag="SCL80_002907"
     tRNA            complement(782..855)
                     /locus_tag="SCL80_002907"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(819..821),aa:Asn,seq:gtt)
BASE COUNT          240 a          238 c          195 g          189 t
ORIGIN      
        1 gtcaaagtcc cttgccttac cgcttggcta taccccaaga ataaaaaggg cgaatgatgg
       61 gaatcgaacc cacgaatgcc ggagccacaa tccggtgcgt taaccacttc gccacattcg
      121 ccatggcagg ggcagcagga attgaaccca cactaacggt tttggagacc gctgttctac
      181 ctttaaacta tgcccctata aaatggagga gagtggattc gaaccactga accctaagga
      241 acggatttac agtccgtcgc gtttagccac ttcgctactc ctccatggtg gcgcgggaca
      301 gaatcgaact gccgacacac ggagcttcaa tccgttgctc taccaactga gctaccgcgc
      361 catattttaa aattattaat tgaataacaa gaacgacggt tccgacggga atcgaacccg
      421 cgatctcctg cgtgacaggc aggcatgtta acccctacac cacggaacca gttcattatg
      481 gaggttaacg ggatcgaacc gctgaccctc tgcttgtaag gcagatgctc tcccagctga
      541 gctaaacctc caacattcaa ttaaaatgac ccgtacggga ttcgaacccg tgttaccgcc
      601 gtgaaagggc ggtgtcttaa ccgcttgacc aacgggccag gtaaatttgc tacggagagt
      661 aagggattcg aacccttgag acagtttggc accatctaca tgatttccaa tcatgctcct
      721 tcggcctctc ggacaactct ccagagtgaa agaagctcaa tactctttct caaaatgaga
      781 actccggcag taggactcga acctacgaca tcatgattaa cagtcatgcg ctactaccaa
      841 ctgagctatg ccggaataac at
//
LOCUS       DARBEC010000239          830 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.251, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000239 DARBEC010000000
VERSION     DARBEC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 830)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 830)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..830
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.251"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            10..>830
                     /locus_tag="SCL80_002908"
     CDS             10..>830
                     /locus_tag="SCL80_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEfa13 family transposase"
                     /protein_id="HEG1108712.1"
                     /translation="MNDFTTEIMEILINKGDLDDLFRRHLELAINALLQAELTVFLDY
                     EKYDRAGFNSGNSRNGNYSRSFKTEYGELNLVIPRDRNGDFSQQTLPAYKRTNDSLET
                     TIIQLVQKGITMSEISDLIEKMYGHYYKPQTISNMSKIVSEDVSAFKERTLEAKYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFTIAPTESAYVWKEILQDLKDRG
                     LEEVLLVVTDGLSGIDDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDF"
BASE COUNT          284 a          123 c          152 g          271 t
ORIGIN      
        1 gacttccata tgaacgattt tactacagaa attatggaaa tactaatcaa taaaggtgat
       61 ttggatgatt tgtttcgtcg tcacctagaa ttggctatta acgctttatt acaagctgaa
      121 ttaacggtat ttcttgacta tgaaaagtat gatagagctg gatttaattc aggtaattcc
      181 cgtaatggga attattcgcg ctcttttaaa acagagtacg gtgaattgaa tttagtaata
      241 cctagagata ggaatggaga cttttctcaa caaactttac ctgcttataa aagaaccaat
      301 gactctttag aaaccactat tattcagcta gttcaaaaag gaattactat gtccgaaatc
      361 tctgatctaa tcgaaaaaat gtatggtcat tattacaaac cacaaactat ttcaaatatg
      421 agtaaaattg tgtctgaaga tgtttcggca tttaaagaaa gaactttaga agctaaatac
      481 tcagtcattt ttatggatgc tactcatatt cccttaaaaa gacaaaccgt atcaaaagaa
      541 gctgtctata tagtaattgg tatcaggttg gatggaacta aagaggttct aggatttact
      601 atagctccaa ctgaatctgc ttatgtttgg aaagagattc ttcaagatct aaaagaccgt
      661 ggtttagaag aggttttgtt agtcgtaaca gatggtttaa gtggtattga cgatagtatt
      721 catagtgttt atccaaatgc tcaatttcaa caatgttgtg tccatatatc tagaaatatc
      781 gctcataagg ttcgtgttag tgatcgacaa gaaatctgta gcgatttcaa
//
LOCUS       DARBEC010000240          767 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.252, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000240 DARBEC010000000
VERSION     DARBEC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.252"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(232..>767)
                     /locus_tag="SCL80_002909"
     CDS             complement(232..>767)
                     /locus_tag="SCL80_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEG1108713.1"
                     /translation="DYPRINRNNTTAFKIFRAIINRCLRMIKLPPKIRHHKFDLYHGS
                     QWWALSKECLTELIQMYEQNQQDYLNFKIGMFAPDEKFFHTLFFNSSFKNKNVIGGPD
                     LPLELKNIEETTLQTSKLANIHIIDPSMNKWFDERDIDQIKESEKLFVRKVKSDYSDK
                     LLNRIDKEILKVGNIDE"
BASE COUNT          270 a          124 c           88 g          285 t
ORIGIN      
        1 ataaaactaa tcattgctcc aatcagtagc caagaaaaac gcattttaaa aaaatttttc
       61 tttataaaaa cctcatacaa aactataatt ccaccccaag caaaagctat tttcatcggg
      121 atattagtta cattaaaagt actaggcata aaataaagca gacaaatgat taaatttatt
      181 actcgaaacg catctcgact taataaactc ttctctaaaa caggtactat tctattcatc
      241 tatatttcca actttcaaaa tttctttatc aattctattt aatagtttat cagagtaatc
      301 cgatttaact tttcttacaa ataatttttc actttctttt atttgatcta tatccctttc
      361 atcaaaccat ttgttcatgg aaggatcaat aatatgaata tttgctaatt tcgaagtttg
      421 taaagtagtt tcttcaatat ttttcaattc aagtggtaaa tctggtcctc caataacatt
      481 tttatttttg aacgaggaat taaaaaaaag tgtatgaaaa aatttttcat ctggggcaaa
      541 catacctatt ttaaaattca ggtagtcttg ttgattttgt tcatacattt gtattaattc
      601 tgttaggcat tctttcgaca aagcccacca ttgagaacca tgatacaaat caaatttatg
      661 gtgtctaatt tttgggggaa gtttaatcat cctcagacaa cgattaatta tagctctaaa
      721 aattttaaat gcagttgtgt tattcctatt aattctagga taatcat
//
LOCUS       DARBEC010000241          759 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.253, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000241 DARBEC010000000
VERSION     DARBEC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..759
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.253"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          266 a          139 c          101 g          253 t
ORIGIN      
        1 gaaccggggg ctttattcct gcagtccggc tacgccttcc tgcaatagac aaaactccag
       61 atcgggtaat tcgcttgtgg cgaaacccga cctggagttt gtctctgtat tttgaaccct
      121 aaaaattaat caaacagact gatattctca taatcagcgc gaaataagcc attaagtaca
      181 gttttcctac ctatttttga taacccgctg gttagttaat attccttttc cgagagagat
      241 aaatgattgg ttaaatctat atacatctca acctttaaaa atgttttcga aatacttcat
      301 cagtacatgt catacaaatt accagtgcat tctcaaattc tagttcttta attgatttca
      361 gttgaggagc agttactttt ggcggaaatt tccatttttg tccaaacctc ttcccccgat
      421 gaaatttctt tgttgcaatt attgcaatga aaaatttttg tttcaaaaat atacataatt
      481 acttcctcca ataaaaacta tctacctctc aaattatttt tctctaataa gaaaaacaga
      541 aaaggtaata agatacaaca gtgatgattc ctaataaaga aaagactaca ataatatttt
      601 tctaacttat cgccttcaaa gaaatagttt cttatttttc actaaaataa aaaatttatt
      661 gcaaaaaaaa tagaagtgac aaaattatca atcatttcaa ggtaatataa ctacctgagt
      721 ttattatcga ttaaaatgct aactacattt gcaagagaa
//
LOCUS       DARBEC010000242          744 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.254, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000242 DARBEC010000000
VERSION     DARBEC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..744
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.254"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..329
                     /locus_tag="SCL80_002910"
     CDS             <1..329
                     /locus_tag="SCL80_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108714.1"
                     /translation="ELSAFTSLRSTYAKTLFRLLMQYRSTGYYVVRIEEFRSLMDIPE
                     YYQMGNIDQKVLNPALRELKKYFNDLKVTKIKAKKGNKIAKLEFTFSSLTSDLPKISL
                     RNWLEE"
     gene            complement(466..>744)
                     /locus_tag="SCL80_002911"
     CDS             complement(466..>744)
                     /locus_tag="SCL80_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108715.1"
                     /translation="DTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGT
                     NSKVYQEVKEKIEAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT          230 a          123 c          130 g          261 t
ORIGIN      
        1 ttgaattatc tgctttcacg tcgttgcgtt ctacgtatgc aaaaactcta ttccgactgc
       61 ttatgcagta ccgttctact ggttattatg tagttcgtat tgaagagttt aggagcttaa
      121 tggatatccc agaatactat caaatgggta acattgatca aaaagtatta aatcctgcat
      181 tacgagaact aaaaaaatac ttcaatgatc ttaaagtcac aaaaataaaa gcaaaaaagg
      241 gcaataaaat cgccaaatta gaatttactt tttcaagcct aacgtcagat ttgccaaaga
      301 tttctctgcg taactggtta gaagaataaa taaaaaagaa ctcgcccccc ttacattaag
      361 caagtagaca agtcccttac aaccgactaa gattgctagt acattctagc atattgtatg
      421 gggcttctaa ataaaaaaca gataggaata actcctatct gttttttatc gttttagaaa
      481 atttgttggg tcgaaatttc tagaagtgac tgtttgactg gtttcttttt gatgattctt
      541 tggtcgattt cgatttgatg gatttagttt agcttcgatt ttttctttca cttcttgata
      601 tactttagaa tttgtgccaa taagaacgcc tgtatctttt agatataaat cagcaaattc
      661 tgcgatgatt tcagtcatga tggaaatttc ttttcccata gcatttagtt ttgcctgatt
      721 gctggtagtg gtcgtgttgg tatc
//
LOCUS       DARBEC010000243          738 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.255, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000243 DARBEC010000000
VERSION     DARBEC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 738)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 738)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..738
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.255"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(91..>738)
                     /locus_tag="SCL80_002912"
     CDS             complement(91..>738)
                     /locus_tag="SCL80_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element ISEfa8 family transposase"
                     /protein_id="HEG1108716.1"
                     /translation="IMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEA
                     IVTDLTYVRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKL
                     FHTDRGKEFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFET
                     LAQLRLELFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          202 a          171 c          140 g          225 t
ORIGIN      
        1 ttgtgaatgc tgcggtgtta tggctacgat tgcccttgac aatgagcatc ctcggatctg
       61 attttctccg gcagaagcac aatcattcaa ttacgctgcc tctccagagg tatcagatcc
      121 gcgctcatta tcaaggattc gcttcgccaa tctctgttgt cgaatttgga tcggtgtttc
      181 atacgccaac gtgccatgta agcgtagata gttccaccaa tgcacataat caaacaattc
      241 tagacgtagc tgtgccagtg tctcaaattg gtattgatgc acgaattcta ctttgacaga
      301 tttataggtt gattccacaa cggcattatc atacggacaa cccttcctac tcaatgaacg
      361 agtaattcca aaaccattca gaatctcatg aatggtttgg ttatcaaatt cctttccccg
      421 gtctgtatga aaaagcttga cgtctgttaa agaatacggt atccgtccaa aggcttcttt
      481 taccaatgag gcatctttct tctcaccaca ggaataacca ataatttctc gattaaacaa
      541 atcaagtatt aagcagatat aatgccactt tttccccacg cgaacataag taagatccgt
      601 aacgatggct tccaatggct gttcttgtgt aaaggtccga tctaatacat tcgctgtttt
      661 cgcttcatta caagctgttc gttgcccttt aaaatgagcg atcgtatagg tagatgtcaa
      721 tccgcggcgt ttcatgat
//
LOCUS       DARBEC010000244          733 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.256, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000244 DARBEC010000000
VERSION     DARBEC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.256"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>733
                     /locus_tag="SCL80_002913"
     rRNA            <1..>733
                     /locus_tag="SCL80_002913"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          226 a          146 c          216 g          145 t
ORIGIN      
        1 attgtgtgaa tacatagcac attcgaggta gacgcagaga actgaaacat ctaagtacct
       61 gcaggaagag aaagaaaatt cgattccctg agtagcggcg agcgaaacgg gaaaagccca
      121 aaccagcaag cttgcttgtt ggggttgtag gactccaata tggtagttct ttcagatagt
      181 cgaatgactt ggaaaagtca gtcaaagagg gtaaaaaccc cgtagacgaa atgtggaaga
      241 cacctaggag gatcctgagt acggcggaac acgagaaatt ccgtcggaat ccgggaggac
      301 catctcccaa ggctaaatac tccctagtga ccgatagtga accagtaccg tgagggaaag
      361 gtgaaaagca ccccggaagg ggagtgaaat agaacctgaa accgtgtgcc tacaacaagt
      421 caaagcccgt taatgggtga tggcgtgcct tttgtagaat gaaccggcga gttacgattg
      481 catgcgaggt taagttgaag agacggagcc gcagcgaaag cgagtctgaa tagggcgttt
      541 gagtatgtag tcgtagaccc gaaaccatgt gatctaccca tgtccaggtt gaaggtgcgg
      601 taaaacgcac tggaggaccg aacccacgta cgttgaaaag tgcggggatg aggtgtgggt
      661 agcggagaaa ttccaaacga acttggagat agctggttct ctccgaaata gctttagggc
      721 tagcctcgga att
//
LOCUS       DARBEC010000245          712 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.257, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000245 DARBEC010000000
VERSION     DARBEC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 712)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 712)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..712
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.257"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..446)
                     /locus_tag="SCL80_002914"
     CDS             complement(<1..446)
                     /locus_tag="SCL80_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108717.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT          216 a          123 c           95 g          278 t
ORIGIN      
        1 aactttctct tttcaaaaaa aatttgatat ttttttagat ttctttttag tctttgtaat
       61 actttataag ccacagaata actcacgtga tgctgtttag caaaattttc tactgagata
      121 tatttttcct gaaagaaagc caataccatt ttcatagata aagaatccgt tatataacga
      181 gataacagag ctttgctaga acattttcca ttaatttcca gacatatatc caattcactg
      241 acggttactc gcaaattttt ctctagatcg aacttttgta aatcatgttt cagcttatcg
      301 atcgtttttt tcaagagaaa tgtcgatagc gacaactctt cttgaacatt cgatattgac
      361 tctgataaat ttgagcttgc ttctaaatat ttaagtaatg aataacttct gtgatcttct
      421 ttgcttaaaa aaaactcata attcattagt catcagctct ttctacgtat attactttcc
      481 atgcattcac caactagctg gggataaaac tgatttcctg gatccgagtc actgttgtca
      541 gcgattcttg tttgtataat atacatcgtc ctgtcttttt tttaacattt tggagagcgt
      601 tttatttggc ctttctcgcg atcaaacctg ttattagaat tatatcattt actagtatgc
      661 atttttatta ataaaatggt atattttata ttgttataaa aagagcccaa cc
//
LOCUS       DARBEC010000246          709 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.258, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000246 DARBEC010000000
VERSION     DARBEC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 709)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 709)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..709
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.258"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..219)
                     /locus_tag="SCL80_002915"
     CDS             complement(<1..219)
                     /locus_tag="SCL80_002915"
                     /inference="COORDINATES: protein motif:HMM:NF024673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108718.1"
                     /translation="MTATEIRNNKLKKKISTIFFTNKQRYGATKIHQVLLKEGISVSL
                     KHVQKLMKQLNLRSIVVKKYRPQRSNKPI"
     gene            complement(330..584)
                     /locus_tag="SCL80_002916"
     CDS             complement(330..584)
                     /locus_tag="SCL80_002916"
                     /inference="COORDINATES: protein motif:HMM:NF013677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108719.1"
                     /translation="MTRYEENFKQMIVELNQTGRSVRGLAKEYGLSEATIYKWKNLYL
                     PDQSTGLTGKEVAELRKENARLNEELEILKKAAAIFSRKT"
BASE COUNT          215 a          127 c          101 g          266 t
ORIGIN      
        1 gatcggttta ttagaccttt gaggtctata ttttttaact acaatcgacc ttaaatttaa
       61 ttgtttcatt agcttttgga catgtttaag agatactgaa atcccttctt ttaataaaac
      121 ttgatggatc tttgtggcac catagcgttg tttattcgta aaaaaaattg ttgaaatctt
      181 ctttttcaac ttattatttc tgatttctgt agctgtcaac ggtttattct tataaaaata
      241 atagacactt ctagggattt ctaataaccg acacaacaaa gaaacagtgt aatccttaca
      301 ccatttttca ataaattgaa caagcgaatt taggttttcc gagagaatat ggctgcggct
      361 ttttttaaga tttcaagttc ctcatttaaa cgagcatttt cttttctcag ttcagctact
      421 tcttttccag tcagtcctgt ggactgatca ggtaaatata aattcttcca tttgtaaatc
      481 gttgcttcag ataagccata ttctttcgct aaccctcgaa cagaacgtcc agtttgattc
      541 agttcaacaa tcatttgttt aaaattttct tcatatcggg tcataaaaat tcatcctctc
      601 gtagataagt ttaatttatt cttgtctcta cgaaaagctg tctaagattt gatactaaca
      661 ccaaatgttg tataccccca aacaatacac atattttgaa ggattagga
//
LOCUS       DARBEC010000247          692 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.259, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000247 DARBEC010000000
VERSION     DARBEC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..692
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.259"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            132..344
                     /locus_tag="SCL80_002917"
     CDS             132..344
                     /locus_tag="SCL80_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014834536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108720.1"
                     /translation="MAVWQIQLKERGAYGLISLSDVLCRKEMPDLFSLDQIIAIIGAA
                     AVVITAVAKASDSILAWLKFKKEQKK"
     gene            506..>692
                     /locus_tag="SCL80_002918"
     CDS             506..>692
                     /locus_tag="SCL80_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000357244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEG1108721.1"
                     /translation="MDKFNRLTLINQFEILKALNPNEEKYYAEKIEILTEGYEYHYSE
                     IFENISDPLPEEDSRFVL"
BASE COUNT          246 a          103 c          122 g          221 t
ORIGIN      
        1 aaaacactat atattccgaa aagtgagtat ctccattcat aaaataatca acatatttaa
       61 aaaatctaat tagctttttt agtcaatcta aagttcttta ttctatgata taatgttact
      121 gtagaaagtt tgtggcggtt tggcaaatcc aattgaagga gcgtggtgcc tatgggctta
      181 ttagcttatc ggacgtactc tgtagaaagg aaatgcctga tttgttctca ttggatcaaa
      241 tcattgctat tatcggagca gcagcagtag ttattactgc cgtagcgaaa gctagcgatt
      301 cgattttagc ttggttaaag ttcaaaaaag aacaaaaaaa atagagccgt ctcaactttt
      361 ggcgaggtag acgagctcta aagctttatt tacatgccat ccgctttgaa cggactacac
      421 tgggcatgtg acagcatgcc ctttcattta tattatatct cattctttta tataatgaaa
      481 gtaaataaat catggaggat aaaatatgga taaatttaat agattaacat taataaatca
      541 gtttgaaatt ttaaaagctt taaatcctaa cgaagaaaaa tactatgctg aaaaaataga
      601 aatactaaca gaaggttacg aatatcacta tagtgaaata tttgaaaata tttctgatcc
      661 attgccagaa gaagattcgc ggtttgttct ag
//
LOCUS       DARBEC010000248          679 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.260, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000248 DARBEC010000000
VERSION     DARBEC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 679)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 679)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..679
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.260"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..212
                     /locus_tag="SCL80_002919"
     CDS             <1..212
                     /locus_tag="SCL80_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ORF6C domain-containing protein"
                     /protein_id="HEG1108722.1"
                     /translation="AYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMW
                     LPETAIRMEIDQLNRQQRLFGDENE"
     gene            386..>679
                     /locus_tag="SCL80_002920"
     CDS             386..>679
                     /locus_tag="SCL80_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108723.1"
                     /translation="MNKELSTLDQYLTDSEWGKSNIKETNNRKIRRNLLTNEELSCDQ
                     DDLGNFVSIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYNRDNLIDISYYG"
BASE COUNT          258 a          114 c          131 g          176 t
ORIGIN      
        1 gagcttatga agaaatcagc aaaaaagtat tctcaaaatt ttggtctgaa tttaaacgta
       61 ccttttcaat cccaagatat ggcgagttac ctcgtaagag attcgatgat gctgtttcat
      121 ttattgaaat gtggttacca gaaactgcga tccgcatgga aatcgatcaa ctgaacagac
      181 aacaaagact tttcggtgat gaaaatgaat agagctgaag cgctaagaat agggacggta
      241 attgctaatc gctggtggag acacaataaa ccaaacatcc taagccaaca acatatcgaa
      301 aagcaaaaag catggcaaca aataaaaaag tgactcagcc ggcaagcata gagtcacaaa
      361 acatatctaa ggagaatttt agcatatgaa taaagaactt tccactttag atcaatattt
      421 gactgattct gaatggggca agtcgaatat caaggaaaca aataatcgaa aaatcagacg
      481 aaatcttttg acgaacgaag aactatcatg tgatcaagat gacttgggaa attttgtgag
      541 tatttgggat catgtttacc ttatccatct atcaaaacat tcaaataaac ctgaatacat
      601 ctatgtcatc gaagatggct tgattgatgc gctagaagag tacaacagag ataacttgat
      661 tgatatctct tattacgga
//
LOCUS       DARBEC010000249          635 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.261, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000249 DARBEC010000000
VERSION     DARBEC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 635)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 635)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..635
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.261"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..270)
                     /locus_tag="SCL80_002921"
     CDS             complement(<1..270)
                     /locus_tag="SCL80_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108724.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFR"
     gene            complement(276..>635)
                     /locus_tag="SCL80_002922"
     CDS             complement(276..>635)
                     /locus_tag="SCL80_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1108725.1"
                     /translation="RNQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPP
                     RIDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQC
                     ALMERAIAAKRKELDKK"
BASE COUNT          164 a          135 c          110 g          226 t
ORIGIN      
        1 acggaagtag aagaattcct tccagaagtc cgttggcatg cctgtatcga taaaaatatc
       61 gcctatcagc gcatagatga agtttcgttg ctgtacggtg aaacgtctag gatcaataaa
      121 acgaatttca atgactcgat caccatcgta gccgtcatac atcgtcttta gtcgatcaat
      181 gttcacttcc tcattgatgg ttgcgcttat gtcttttcct ttgaactttt tcagaaccgc
      241 tgaatatgaa tcgattaatg gtttaaacac tcatatcact tcttatctaa ttcttttctc
      301 ttagctgcta ttgctcgctc catcaaggca cattgctcat agcttaactg ttcaatagtt
      361 tcaacgttat cagctaagag ccctaattta tctgtctgct cattaacata ttcgattaag
      421 gttttggtca tatctttacc catctgctca ttgaaagctt ctagaatcgt ctctagcatg
      481 tttaatttct ttgtatcgat tctaggtggt gttggaatat cttccccttg aaatacatat
      541 aatcccagtc cgtgtagagc caatgctttc acaaagcatc gcttcaatga gttattgatt
      601 tgcattgcat ttggtttaac aactggttgg tttcg
//
LOCUS       DARBEC010000250          596 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.262, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000250 DARBEC010000000
VERSION     DARBEC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.262"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            134..532
                     /gene="spxA"
                     /locus_tag="SCL80_002923"
     CDS             134..532
                     /gene="spxA"
                     /locus_tag="SCL80_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEG1108726.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
BASE COUNT          208 a           92 c          119 g          177 t
ORIGIN      
        1 cattttttta aaaaaattat gaaaaaaagg tagctattta aaagttgatt atgtataata
       61 gaaacagatg ggagattatt atgatcttct gttacaaaaa agtgattctt atcacgaaaa
      121 ggagtggtgc acgttgttaa cattatatac ttccccaagt tgtacttcct gcagaaaggc
      181 tcgtgcgtgg ttgcaagaac atcagattcc atttgttgaa cgtaatattt tttcagaacc
      241 attgaacagt tctgaattaa aagcgatttt acaaatgaca gaagacggaa cagaagaaat
      301 catctcgaca cgttccaaag tattccaaaa actgaacatg gatttagatg atctgccgtt
      361 acaagaatta ttggaacttg tgcaaaacaa tcctggattg cttcgccgtc caatcatgat
      421 tgatgataaa cgtctgcaag ttggttttaa tgaagatgaa attcgtcggt tcctaccgcg
      481 cgatgtacga caattagaat tacgtcaagc acaattgatg gctggattat agaaggataa
      541 tacgaaactt ggaaaagact ggctctttgt caaatgatgt tggtaaagag aaatct
//
LOCUS       DARBEC010000251          588 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.263, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000251 DARBEC010000000
VERSION     DARBEC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 588)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 588)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..588
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.263"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(65..150)
                     /locus_tag="SCL80_002924"
     tRNA            complement(65..150)
                     /locus_tag="SCL80_002924"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Leu,seq:taa)
     gene            complement(167..238)
                     /locus_tag="SCL80_002925"
     tRNA            complement(167..238)
                     /locus_tag="SCL80_002925"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(204..206),aa:Gly,seq:gcc)
     gene            complement(250..322)
                     /locus_tag="SCL80_002926"
     tRNA            complement(250..322)
                     /locus_tag="SCL80_002926"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(287..289),aa:Thr,seq:tgt)
     gene            complement(340..421)
                     /locus_tag="SCL80_002927"
     tRNA            complement(340..421)
                     /locus_tag="SCL80_002927"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(385..387),aa:Leu,seq:tag)
     gene            complement(429..501)
                     /locus_tag="SCL80_002928"
     tRNA            complement(429..501)
                     /locus_tag="SCL80_002928"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(466..468),aa:Lys,seq:ttt)
     gene            complement(505..577)
                     /locus_tag="SCL80_002929"
     tRNA            complement(505..577)
                     /locus_tag="SCL80_002929"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(542..544),aa:Val,seq:tac)
BASE COUNT          149 a          169 c          138 g          132 t
ORIGIN      
        1 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattt
       61 gaaatgccga ggaccggaat cgaaccggta cggtgatcac tcaccgcagg attttaagtc
      121 ctgtgcgtct gccagttccg ccaccccggc gcgattgctt tggcaaagcg gaaaacgggg
      181 ttcgaacccg cgacccccac cttggcaagg tggtgctcta ccactgagct atttccgcgt
      241 gtttaaagat gccggctaaa ggacttgaac cctcgaccct ctgattacaa atcagatgct
      301 ctaccaactg agctaagccg gcatgtaaaa cttaaattat gcgggtgaag ggacttgaac
      361 ccccacgccg taaggcgcta gatcctaaat ctagtgcgtc tgccaattcc gccacacccg
      421 caaaaatatg agccgtacag ggctcgaacc tgtgaccctc tgattaaaag tcagatgctc
      481 taccaactga gctaacggct catatggagg ttaacgggat cgaaccgctg accctctgct
      541 tgtaaggcag atgctctccc agctgagcta aacctccatg gataactt
//
LOCUS       DARBEC010000252          556 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.264, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000252 DARBEC010000000
VERSION     DARBEC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 556)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 556)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..556
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.264"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..221)
                     /locus_tag="SCL80_002930"
     CDS             complement(<1..221)
                     /locus_tag="SCL80_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108727.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNK"
BASE COUNT          191 a          101 c           55 g          209 t
ORIGIN      
        1 tccttattat tagatactaa ttgatcaatc atagggagcc attttttgat tttcactaat
       61 tgattgctca taaatctttc actggacttt tgttcaatct taaattgcaa aacttctaac
      121 tcataattcc catcttctga tttatttttg ggaacaacta tcaaactttt ataaagttta
      181 tccacatcaa aatttatctt ttttgcataa tttgtagcca ttaatacatc tccttattca
      241 taataaattt catatgacgc tcctctcaaa atttaccggt agactagaaa aaagaataaa
      301 agatacgtca attcacactt tattcttaaa aaaaaactgg tctctctacc ggtaatatcg
      361 attgagtccg cctttatcga ctttatcctt tttttatatt aaattttttc accggtagac
      421 agggagagaa agaataaact aaaaagccct taattcttaa tgttttcacc tccttaccgt
      481 tcctaccgct aaaatttaat ataactaaat tctataacat tagaagaaat tttaaatacc
      541 tttgataaaa aatatt
//
LOCUS       DARBEC010000253          526 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.265, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000253 DARBEC010000000
VERSION     DARBEC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 526)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 526)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..526
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.265"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCL80_002931"
     CDS             complement(6..359)
                     /gene="tnpB"
                     /locus_tag="SCL80_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEG1108728.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(349..>526)
                     /locus_tag="SCL80_002932"
     CDS             complement(349..>526)
                     /locus_tag="SCL80_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108729.1"
                     /translation="IVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIYNGINNY
                     ILQAILKELLSNAL"
BASE COUNT          153 a          116 c           90 g          167 t
ORIGIN      
        1 ccagcttaaa aaactccttt ttttcctggc aaaatagctc gaggttgatc gatacttaat
       61 ccttctaaca accatcgaac ctgttggttc gttaaggttc gaacttcgtc tttcgtccga
      121 ggccatttta acttgccgtt ttctatccgc ttataacaga gaagaaaacc atcaccgtcc
      181 caatagagaa gtttgaaacg gtcttgacgg ttcccgcaaa ataaaaaaac ggcatcttcg
      241 taaagctcga gttgatactc ttgttgaata agactggcaa gaccatcaat tccttttcgc
      301 aaatctgttt ttccgcacac aatataaata tgtttggttt gaagtaaatc ataaagcatt
      361 ggacaacagt tctttcaaaa tagcttgaag aatataattg ttaataccgt tatagatcaa
      421 aacttcagaa ccattctctc ttttgatgcg catcgctaac ttggcagccg cgggtttacc
      481 tttttctggt gcactctttt cgtttcctaa ttggacagga acaatc
//
LOCUS       DARBEC010000254          517 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.267, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000254 DARBEC010000000
VERSION     DARBEC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..517
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.267"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>517
                     /locus_tag="SCL80_002933"
     CDS             <1..>517
                     /locus_tag="SCL80_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108730.1"
                     /translation="DLQERGLKNVLLFITDGLKGMVGAISRFYPKARFQHCCVHVSRN
                     ISHKVRVDDRKEVCDDFKMVYQASSKEVALEARGTFAEKWKTSYPKVVESILSNDHLL
                     TFYDFPLAIRKSIYSTNLIESFNKQIKKYSHRKEQFQNEESMERFLVSSFDTYNQKFL
                     GRSHKGFQQAEG"
BASE COUNT          152 a          104 c          114 g          147 t
ORIGIN      
        1 gaccttcaag agcgcggttt gaaaaatgtc ctcctgttca tcacggatgg cttaaagggg
       61 atggtaggag cgattagtcg gttctatccc aaagctcgtt ttcaacattg ttgtgtacac
      121 gtttcccgta atatcagtca taaagtgcgt gtcgatgatc gtaaggaagt ctgtgatgat
      181 tttaaaatgg tgtatcaagc ctcatctaaa gaggtggcgt tggaagcacg tggtactttt
      241 gcggaaaaat ggaaaaccag ctatccaaaa gtggttgaat cgattctttc gaacgatcac
      301 ttgctcactt tctatgattt ccccttggcc atacgcaaga gtatttattc tacgaacttg
      361 attgaatcct ttaataaaca aatcaagaaa tacagccacc gcaaggaaca gttccaaaat
      421 gaagagtcga tggaacgttt cttagtctcg tcttttgata cttacaacca aaaattccta
      481 ggtcgcagtc ataaaggttt ccaacaggcc gaaggcg
//
LOCUS       DARBEC010000255          511 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.268, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000255 DARBEC010000000
VERSION     DARBEC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.268"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          157 a           76 c           78 g          200 t
ORIGIN      
        1 tttacttgag gagtaaaaat gaaaaagtat atagggttgt tggttggtat gtatatagaa
       61 tagaagataa atgtgaagaa attgtgcatt atttcttcac atttattagg ttatagttta
      121 agaaagttta aacattgcgc aatttaatta ttttagctgt ttcttttcat aaaaaaacat
      181 gttgtgactc tgagacaaat catagtccat agtgatgtct ctagagtaac tacgcctaat
      241 attacgaggc atcatattaa tctatcagca ctagtaccta aactagcatc cctttcgaat
      301 attttttctt atataataaa gtttgctttc actcctctaa tctcaccaaa tcgactggct
      361 tgcgttgtgt gatactagga actgattggt ttagaacgac ttttctattg taatcatatt
      421 tttcgccatc ctcgttgtct tctaatcctt caatgaatga tccctaatgt gtgtattaaa
      481 taatttaatt tttttgttcc tatttattta a
//
LOCUS       DARBEC010000256          511 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.269, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000256 DARBEC010000000
VERSION     DARBEC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 511)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 511)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..511
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.269"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            144..401
                     /locus_tag="SCL80_002934"
     CDS             144..401
                     /locus_tag="SCL80_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108731.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
BASE COUNT          214 a           65 c           71 g          161 t
ORIGIN      
        1 tttttctgtc cacgttcatt ttactccaaa gttttcctca tttcaaaaga aaatcacaga
       61 atacacgaag aagcgtttct atttgtataa actatatcaa agttctatac ttaaattatc
      121 aaataaaaag gacgtgaaga taaatgtcaa atttagaaaa caaagaagaa aaagttgtaa
      181 acaaaattgt ttctgttgtc aataaactgg ataaagagtt agacgaactt gatacgttat
      241 ctgaaaatcc agaaaaaaaa cataacctaa aaaaatggtt agttgaacga aaggctattc
      301 atgaaattaa aaaagttctt catgaagctg ataaatatga aaaatatgat gaaaaagaac
      361 ttgataaaga atttaaagaa atcaatgact tattactata attgatttat acgactcatt
      421 tttgagtcgt cagactgcaa acaacactct aaatttagga gtttgtctac agtctgaata
      481 gattttaaat ttattgtcca taatgttatt a
//
LOCUS       DARBEC010000257          508 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.270, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000257 DARBEC010000000
VERSION     DARBEC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..508
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.270"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..452)
                     /locus_tag="SCL80_002935"
     CDS             complement(<1..452)
                     /locus_tag="SCL80_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108732.1"
                     /translation="MNDFTTEILKTLANKGDLNELFRVHLEKAVNTLLKTELTAFLDY
                     EKYDRIGFNTGNSRNGSYDRTVKTEYGELHLQIPRDRNGEFKQQTVPAYRRTNDTLEE
                     TVIHLFRKGITMSEIADLIEKMYGHYYTPQTMSNITKSFTEEVTAFNGR"
BASE COUNT          126 a          110 c          112 g          160 t
ORIGIN      
        1 cgcccattaa acgccgttac ctcttctgta aatgattttg ttatattgga catggtttgg
       61 ggcgtgtagt aatgcccata cattttctca atcaagtctg cgatttccga catggtaata
      121 ccttttcgga agaggtgaat gacggtctcc tctaacgtgt cattcgtccg tctataagca
      181 ggaacagtct gttgcttgaa ctcaccattg cggtcgcgcg gaatctggag atgaagttcc
      241 ccatactcgg tcttgaccgt acggtcatag gagccgttac gagaattacc cgtgttaaaa
      301 ccaatgcgat catacttttc gtaatctagg aaagccgtta actccgtttt gagaagcgta
      361 ttgactgctt tctccaaatg gacacggaat aattcattca aatcgccttt gttcgctaga
      421 gtctttagaa tttctgtagt aaaatcgttc atagggaagt cctctcttct gtgaatgtgt
      481 tgtggtaact ctattctaca gaagggac
//
LOCUS       DARBEC010000258          507 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.271, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000258 DARBEC010000000
VERSION     DARBEC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 507)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 507)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..507
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.271"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..506
                     /locus_tag="SCL80_002936"
     CDS             <1..506
                     /locus_tag="SCL80_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1108733.1"
                     /translation="YLWKKKNRQSFYSWKMDETYIKIKGRWHYLYRAIDADGLTLDIW
                     LRKKRDTQAAYAFLKRLHKQFGEPKAIVTDKAPSLGSAFRKLQSVGLYTKTEHRTVKY
                     LNNLIEQDHRPIKRRNKFYQSLRTASSTIKGMETIRGIYKKNRRNGTLFGFSVSTEIK
                     VLMGITA"
BASE COUNT          176 a           96 c          103 g          132 t
ORIGIN      
        1 attatctttg gaagaagaaa aatagacaat ccttctattc atggaaaatg gacgaaacct
       61 atatcaaaat taagggacgt tggcattatc tttatcgtgc aattgatgcg gacggcttaa
      121 ccttagatat ctggttacga aagaaacggg atacgcaagc agcctatgct ttcttaaaac
      181 gactccataa acagtttggt gagccgaaag caattgtgac cgataaagca ccttctcttg
      241 gctccgcctt tagaaagtta cagagtgtgg gtttatatac taagacagag caccgaactg
      301 tgaagtatct taacaattta atagaacaag accatcgacc tattaaacga cgaaataaat
      361 tttatcaaag tctccgtaca gcctcttcca cgattaaggg catggagacc attcgaggaa
      421 tatataaaaa gaaccgaaga aatggaacgc tcttcggctt ttcggtgtct actgaaatca
      481 aggtattaat gggaataaca gcctaag
//
LOCUS       DARBEC010000259          501 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.272, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000259 DARBEC010000000
VERSION     DARBEC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.272"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..124)
                     /locus_tag="SCL80_002937"
     CDS             complement(<1..124)
                     /locus_tag="SCL80_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108734.1"
                     /translation="MKLFVGIDVSSEKLDVCFLTDDNQLSILSEISVANDIEGAS"
BASE COUNT          181 a           92 c           89 g          139 t
ORIGIN      
        1 atgaagcacc ttcgatgtca ttggcaacag agatttcaga taagatagac aattgattgt
       61 cgtctgttaa aaaacaaaca tctagttttt cagagctaac gtcaataccg acaaataatt
      121 tcatggaaga aggacctcct ttcgatataa attttggaaa atgcttcgat actgtgggat
      181 ttcccagaaa ctcactgtat agccacaacc tcgcctaata gaatacggtg ataaaagagc
      241 tagaagctgc cttccaaact actatttgaa agtctagtcc attatcgaaa cagctaatga
      301 gttggtagtg ataacagtgg ttcgggtaac agactaaaca gggtagacaa acgctacagg
      361 aggaaatagc aaattcccgt tggatccttt ccatacatcc attgtactct gagaaatcac
      421 acaatatcga aacaaattcc gttaggaatg tgttattcaa taaattttat aatctagaaa
      481 cattttgaat aaaaactatt a
//
LOCUS       DARBEC010000260          485 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.273, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000260 DARBEC010000000
VERSION     DARBEC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..485
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.273"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(62..259)
                     /locus_tag="SCL80_002938"
     CDS             complement(62..259)
                     /locus_tag="SCL80_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEG1108735.1"
                     /translation="MNNGTVKWFNSDKGFGFITGEDGNDVFAHFSAIQGEGFKSLDEG
                     QAVTYDIEEGQRGPQAVNIVK"
BASE COUNT          158 a           89 c           68 g          170 t
ORIGIN      
        1 gaattttttc taccgacata aacaaaaaaa ccacctcata tgaggcggtt aacgttcaac
       61 attattttac gatatttact gcttgaggac cacgttgtcc ttcttcaata tcataagtaa
      121 ctgcttggcc ttcatctaaa gacttaaagc cttctccttg gattgctgag aaatgagcga
      181 atacgtcatt tccatcttca ccagtgatga aaccaaaacc tttatctgag ttaaaccatt
      241 ttactgtacc gttattcata tatatttcct cctgatacgt atataatacg tgttttattg
      301 caattaatat tacttggtaa aaggagtttt tatagtgtga atatatcgct cagattacgt
      361 ttcaaattag attacttatt tactataaca cggatgtaat gctaacacaa ggataatcac
      421 tcgatatacg gtttaaatat tattatttgt caaattaagc gagacaaaac tttccattta
      481 cgtaa
//
LOCUS       DARBEC010000261          475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.274, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000261 DARBEC010000000
VERSION     DARBEC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.274"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            83..>475
                     /locus_tag="SCL80_002939"
     CDS             83..>475
                     /locus_tag="SCL80_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEG1108736.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRF"
BASE COUNT          165 a           53 c           92 g          165 t
ORIGIN      
        1 atccttttgt ataataacaa ccaacctatt tacgagttgg ttgttaaagg ttattattta
       61 tttgtaaggg gggaagaaag gaatggatga aaaaacggaa ttaacagtca aatatcaaaa
      121 tgaattgaat ttagtaccgc tgaaaaattt caatgctaag cagatggatt tattttttgc
      181 attatgcgct cgtatgaaag atcgtggttt gaaaaaagtt agattttctt tcgaggaatt
      241 aaaagagtta agtgattata agatgactgc tacaaaagcc tttgtcggtg atttagaaaa
      301 gctctataaa gatatgctta atcttactta ccgtactgaa gatgaggatt ctattcgcta
      361 ctttgttttg tttactggtt ttgaaatcaa taaaacaaaa aagtttgttg aagtcagtgt
      421 taatccggac ctagaatata ttattaatgg tttaacagct gaatttagta gattt
//
LOCUS       DARBEC010000262          475 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.275, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000262 DARBEC010000000
VERSION     DARBEC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.275"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>475
                     /locus_tag="SCL80_002940"
     CDS             <1..>475
                     /locus_tag="SCL80_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108737.1"
                     /translation="TAGEGTSIESGTTVFAVKDGVSLPEDKLPVLKAKDGYTDAKWPE
                     EATQPITADDTEFVSSATKLDDKSDADKYNPEGQKVTTELNKEPDAFEGIKNKEDLPK
                     DTKYTWKEKVDVSAAGNKKGTVVVTYPDGSSDEVEVDVTVTDNRSDADKYEPTVEG"
BASE COUNT          192 a           72 c          112 g           99 t
ORIGIN      
        1 tcaccgcagg agaaggaaca agtattgaaa gtggaacaac agtctttgca gtgaaagacg
       61 gcgtaagctt accagaagat aaacttccag tgttgaaagc aaaagatggt tatacagatg
      121 cgaaatggcc agaggaagca actcaaccaa ttacagcaga tgatacagaa tttgtatcaa
      181 gtgcaacaaa actggatgat aaatctgacg ctgacaaata taatcctgaa ggtcaaaaag
      241 tgactacaga attgaataag gaaccagacg catttgaggg aattaagaac aaagaagatt
      301 taccaaaaga tactaagtat acttggaaag aaaaagtaga tgttagcgca gctggaaata
      361 aaaaagggac tgttgtagtg acatatccag atggatcatc tgatgaagtt gaagtagatg
      421 ttacagtgac agacaatcgt tctgacgccg ataaatatga gccaacagta gaagg
//
LOCUS       DARBEC010000263          460 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.276, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000263 DARBEC010000000
VERSION     DARBEC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 460)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 460)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..460
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.276"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(20..91)
                     /locus_tag="SCL80_002941"
     tRNA            complement(20..91)
                     /locus_tag="SCL80_002941"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(56..58),aa:Glu,seq:ttc)
     gene            complement(113..200)
                     /locus_tag="SCL80_002942"
     tRNA            complement(113..200)
                     /locus_tag="SCL80_002942"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(164..166),aa:Ser,seq:gct)
     gene            complement(225..298)
                     /locus_tag="SCL80_002943"
     tRNA            complement(225..298)
                     /locus_tag="SCL80_002943"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(262..264),aa:Ile,seq:gat)
     gene            complement(312..382)
                     /locus_tag="SCL80_002944"
     tRNA            complement(312..382)
                     /locus_tag="SCL80_002944"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(348..350),aa:Gly,seq:tcc)
     gene            complement(384..459)
                     /locus_tag="SCL80_002945"
     tRNA            complement(384..459)
                     /locus_tag="SCL80_002945"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(424..426),aa:Asp,seq:gtc)
BASE COUNT          132 a          128 c          108 g           92 t
ORIGIN      
        1 taaaactata ttaaattaat gacccgtacg ggattcgaac ccgtgttacc gccgtgaaag
       61 ggcggtgtct taaccgcttg accaacgggc caggtaaaac aaaatattga aacggagaag
      121 gagggatttg aaccctcgcg ccggtttccc gacctacacc cttagcaggg gcgcctcttc
      181 agccacttga gtacttcccc aaaaccaata agattttgta ctaatgggcc taaatggact
      241 cgaaccatcg acctcacgct tatcaggcgt gcgctctaac cagctgagct ataggcccat
      301 aaaaaactaa aagcgggtga cgagaatcga actcgcgaca acagcttgga aggctgtggt
      361 tttaccacta aactacaccc gcatggcggt tccgacggga atcgaacccg cgatctcctg
      421 cgtgacaggc aggcatgtta acccctacac cacggaacca
//
LOCUS       DARBEC010000264          455 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.277, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000264 DARBEC010000000
VERSION     DARBEC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 455)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 455)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..455
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.277"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            78..281
                     /locus_tag="SCL80_002946"
     CDS             78..281
                     /locus_tag="SCL80_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEG1108738.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            281..>455
                     /locus_tag="SCL80_002947"
     CDS             281..>455
                     /locus_tag="SCL80_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEG1108739.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLV"
BASE COUNT          184 a           48 c           87 g          136 t
ORIGIN      
        1 gatatttaat attacaagca atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa
       61 gaaagaaaaa gaaagtattg aaacattgtg ttattctagg aatattagga acttgtctag
      121 ctggcattgg tacaggaata gacgttgacg cagctactta ctatggaaat ggtctttatt
      181 gtaacaaaga aaaatgttgg gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt
      241 ggggagataa tctttttggt agttttagtg gaggacgata atgacttcaa taaacattga
      301 tcacacaaaa aaagtcatac atgaattata caatagttta atgacacgac ctaatcagac
      361 gaaagcatta ttagatatta ctgatgtttt atttcaggta tataaaaaaa ttgatacagc
      421 aaaatatcct gaaattcttg taaataaatt agtta
//
LOCUS       DARBEC010000265          429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.278, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000265 DARBEC010000000
VERSION     DARBEC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.278"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>429
                     /locus_tag="SCL80_002948"
     CDS             <1..>429
                     /locus_tag="SCL80_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108740.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSAHTSCDDNGFILST"
BASE COUNT          157 a           75 c           88 g          109 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgcc catacttcat gcgacgacaa tggatttatt
      421 cttagcaca
//
LOCUS       DARBEC010000266          429 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.279, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000266 DARBEC010000000
VERSION     DARBEC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 429)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 429)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..429
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.279"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            8..424
                     /locus_tag="SCL80_002949"
     CDS             8..424
                     /locus_tag="SCL80_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEG1108741.1"
                     /translation="MMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVLSASIP
                     ILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEKLLYIT
                     KTSPYNKTDEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          177 a           56 c           57 g          139 t
ORIGIN      
        1 agagaaaatg atggatcaaa aagcgtttat cgaatctatt gattctacaa tcgatagatt
       61 aaaaaaagaa attcgatttt ataatagagt aattggtatc ggaaatatta taaaaatagt
      121 cttatcagct agtatcccaa tattaattga tcaggcttcc gaacacaggt ctttactatt
      181 gattgtttca attgcttcag caataattac aattatacaa agtggtatgt ctgcatttaa
      241 ttatcaagat aaagtacaga cttcgacaga gttattaatg aaaattgaaa aagaaaagtt
      301 gttatatata acgaaaacat ccccatataa taaaacagat gaagaaaatt tccatttaat
      361 cgttacaacc cttcaaactg aattaaatga tattatctct gattttaatc aagttaataa
      421 ctaaaaatt
//
LOCUS       DARBEC010000267          415 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.280, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000267 DARBEC010000000
VERSION     DARBEC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.280"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..339)
                     /locus_tag="SCL80_002950"
     CDS             complement(<1..339)
                     /locus_tag="SCL80_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEG1108742.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYT"
BASE COUNT          116 a           70 c           68 g          161 t
ORIGIN      
        1 tgtataactc tgaacaggat ttttcttact tttgaaacgt ggaaatccaa cggatttatc
       61 ccgaaaaaaa ttcttgtatg ctttatctaa attaagttgg gcattggcta aagcaaggct
      121 atcaatttct ttcaaaaacg gaaattcttt cttgtattta gctggtgtcg gaaaagtcat
      181 ttttttagaa gaatcatttt taacttcttc atacgccttt tttcggtcat caagcattag
      241 attgtagacc ttacggacac aaccaaaaga tttggcaaag aagatttctt gttcctctgt
      301 tggatagatt ctgaatttgt atgcttttag ttgttccata aagttcacct actttctatt
      361 tatgattgtt tccttgattt tggatatact tcttaatcac atcaattggt gcgcc
//
LOCUS       DARBEC010000268          401 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.281, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000268 DARBEC010000000
VERSION     DARBEC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 401)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 401)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..401
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.281"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          112 a           98 c           76 g          115 t
ORIGIN      
        1 attataaata actcataaaa aatattttga ctttcaaaat cacttttgct accagaagtt
       61 gaggcgaagt gttttaagca tagttgtacc ctagtttgac cgttaaaaat aaataacgat
      121 cgctagagtg caactatgtt tgtttgccct accgtcagcg tcggcttggc ctctcacatc
      181 cattaccgtc gggattatct ccgttacata cccttacaaa atacgccctg taagcattgc
      241 atgaaaccac cgtgcatttg caccgtattt ccgccctagc cgttcaaatc ggtcgctacc
      301 accattcaga aacacgtgcg gtaatggaag gtttggagga agctatccag accactaacg
      361 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DARBEC010000269          381 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.282, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000269 DARBEC010000000
VERSION     DARBEC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 381)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 381)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..381
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.282"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(21..94)
                     /locus_tag="SCL80_002951"
     tRNA            complement(21..94)
                     /locus_tag="SCL80_002951"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ile,seq:cat)
     gene            complement(108..181)
                     /locus_tag="SCL80_002952"
     tRNA            complement(108..181)
                     /locus_tag="SCL80_002952"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(145..147),aa:Met,seq:cat)
     gene            complement(209..281)
                     /locus_tag="SCL80_002953"
     tRNA            complement(209..281)
                     /locus_tag="SCL80_002953"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(246..248),aa:Ala,seq:tgc)
     gene            complement(301..374)
                     /locus_tag="SCL80_002954"
     tRNA            complement(301..374)
                     /locus_tag="SCL80_002954"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(338..340),aa:Pro,seq:tgg)
BASE COUNT          103 a          108 c           90 g           80 t
ORIGIN      
        1 tgaaacttac aacgccacaa tggaccttgt aggactcgaa cctacgaccg gacggttatg
       61 agccgtctgc tctaaccaac tgagctaaag gtccaggctc ttaaaaatcg cggcggaggg
      121 gatcgaaccc ccgacctccc gggtatgaac cggacgctct agccagctga gctacaccgc
      181 gaaaaagata ttcattattt aaatgaaatg gagcctagcg ggatcgaacc gctgacctcc
      241 tgcgtgcaaa gcaggcgctc tcccagctga gctaaggccc cgtgtttaaa tttataatta
      301 tcgggaagac aggattcgaa cctgcgaccc cttggtccca aaccaagtgc tctaccaagc
      361 tgagctactt cccgtttaat t
//
LOCUS       DARBEC010000270          327 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.283, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000270 DARBEC010000000
VERSION     DARBEC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.283"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(74..>327)
                     /locus_tag="SCL80_002955"
     CDS             complement(74..>327)
                     /locus_tag="SCL80_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1108743.1"
                     /translation="NKLKEYKIFQSMSRKGNCLDNSLMENFFGLLKQEIFHGKVYNCF
                     EELKSAIDSYIYYYNNERIKQKLNWQSPVQFRKTTVTVV"
BASE COUNT           94 a           59 c           51 g          123 t
ORIGIN      
        1 agtgaccccc aaaagttaga cttttttgga gtggagaaat ccactctttt tttcatgctc
       61 taaaatgata tttttacaca accgtaacgg ttgttttacg aaattggacg ggactttgcc
      121 agtttaattt ctgttttatc cgttcattat tgtagtaata gatatagcta tcgatggctg
      181 attttaattc ctcgaaacag ttataaactt tcccgtgaaa aatctcttgt tttaataagc
      241 caaaaaaatt ctccataagt gaattatcta agcagttccc tttccgagac atgctttgaa
      301 agattttgta ctccttcaat ttgtttc
//
LOCUS       DARBEC010000271          324 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.284, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000271 DARBEC010000000
VERSION     DARBEC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 324)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 324)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..324
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.284"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          145 a           42 c           51 g           86 t
ORIGIN      
        1 attccttagg cagcttttgc tgtgtagaaa ggaataaata ataacgaaag ttcaaaagct
       61 aatgccgact attctagaca ttaaagaaga cgaaaacgaa cgataaagaa agaaaagtta
      121 tgaataatcg tttattgaaa aaaacttgaa aaaatacaaa aaattcgttg accaagctaa
      181 atttagttgg tatattatta cttgtccaaa agacagaaga aaatatttat tcaaaaacat
      241 aacaaaaaag ttattgacat gttactaacg acatgataag ataactgagt cgttaaaaaa
      301 gaaccacttg cgaagcttga ttct
//
LOCUS       DARBEC010000272          321 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.285, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000272 DARBEC010000000
VERSION     DARBEC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.285"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(69..321)
                     /locus_tag="SCL80_002956"
                     /pseudo
     CDS             complement(69..321)
                     /locus_tag="SCL80_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298938.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT           79 a           78 c           51 g          113 t
ORIGIN      
        1 tccaaactcg taactcgagc ttggaacagc tttttatcta cttcgtttcg aatttttgaa
       61 caaaaaaata aaggctcctt gcctttccat agccaggtgg ctaatttctt taaattcatt
      121 gcagcaaaca ctagcatcgt atgcgtcgcg actttttcca agcctcgata cttggtccaa
      181 cgcatcccat gcttttcttt tgcatccgca aatattcgtt caatcgtttg tttccgtttt
      241 ttgtaaattg atttattcag atctgttagg cgaagatgtt ccacttcatc catcagatct
      301 ccccaaacat gtctcgtgat g
//
LOCUS       DARBEC010000273          308 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.286, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000273 DARBEC010000000
VERSION     DARBEC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 308)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 308)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..308
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.286"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            19..>308
                     /locus_tag="SCL80_002957"
     CDS             19..>308
                     /locus_tag="SCL80_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEG1108744.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTET"
BASE COUNT          112 a           44 c           66 g           86 t
ORIGIN      
        1 aaatggaggc agaatttgat gagtgaaatc aaagggacaa cgaactttga aaaacttttt
       61 agtcgtaagt taaataaaat tctcaagaaa aaaggaaatt ttgattattt atcttgggct
      121 cacgcgtggg agattatgaa aaagaatgat ccacaggcaa cggtaactat taatgagtac
      181 aaacactaca gggttgtttc tggaactcat caagactttc ttgttgagga atataaacct
      241 tttcttatgg atgaaactgg gacttatgta tctgtctcag taacggttaa aggacacacg
      301 gaaaccga
//
LOCUS       DARBEC010000274          307 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.287, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000274 DARBEC010000000
VERSION     DARBEC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..307
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.287"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(76..>307)
                     /locus_tag="SCL80_002958"
     CDS             complement(76..>307)
                     /locus_tag="SCL80_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108745.1"
                     /translation="LKNLPETLDAQLRTKLQNLLTYEEGIYNAMIYPYSNGKIEAKIP
                     HIKTLKRLSYGFKSFENMKIRIFLINQLIQVK"
BASE COUNT           93 a           46 c           56 g          112 t
ORIGIN      
        1 atgttggtga agttcaccaa catcaaaaag tatagagccg atagaaggac tgaccttctg
       61 tctcagattt ttttgttatt tcacttgaat caattgattg atcaaaaaaa tcctgatttt
      121 catgttctca aatgatttaa atccataaga taatcgtttc agtgtcttga tatggggaat
      181 cttggcctct attttgccat tagaatacgg gtaaatcata gcattgtaaa taccttcttc
      241 atacgttaaa aggttttgaa gtttcgtgcg aagttgtgca tcaagtgttt caggaagatt
      301 ttttaat
//
LOCUS       DARBEC010000275          305 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.288, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000275 DARBEC010000000
VERSION     DARBEC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.288"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCL80_002959"
     CDS             complement(<1..302)
                     /gene="tnpA"
                     /locus_tag="SCL80_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEG1108746.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLW"
BASE COUNT           95 a           45 c           50 g          115 t
ORIGIN      
        1 ttccaaagaa attgtttgac tcttggaaag tcacgtttta tcaatcttga gctggcactt
       61 ttataggcat tgatgaattt tgtcaattct gttttaggtt gagctttgaa cataatatga
      121 acgtggtctt tatcatgatt ccactccact aaagtaatgt gatacgattc tgaaattctt
      181 tcaaaagtag ttttggcata atcagatatt tcatcatcaa tcacttgtct acgatatttc
      241 gttactaaaa caaggtggta ataaagaaga aatactgaat gattatttgt atctaattcc
      301 atgtt
//
LOCUS       DARBEC010000276          291 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.289, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000276 DARBEC010000000
VERSION     DARBEC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..291
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.289"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>291
                     /locus_tag="SCL80_002960"
     CDS             <1..>291
                     /locus_tag="SCL80_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS5/IS1182 family transposase"
                     /protein_id="HEG1108747.1"
                     /translation="LVKQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRP
                     KGKKGLLSKNEFLYDEYFDCYICPQDQMLAFSTVTREGYREYKSNPKECVNC"
BASE COUNT           86 a           55 c           58 g           92 t
ORIGIN      
        1 agctcgtcaa acaatcgaag agactttttc ctgaaatcaa ttgtgttgtc gctgatgctg
       61 ggtataagac acctaagttt gttcactttt tgactcattt gaaccttcga ccttgtttgc
      121 cttattcaag gcccaaaggg aaaaaggggc tattatcaaa aaatgagttt ctctatgacg
      181 agtattttga ttgttatatt tgtccgcaag atcaaatgct cgctttttca acggttactc
      241 gagaagggta ccgagaatac aaatccaatc caaaagaatg tgtgaactgt c
//
LOCUS       DARBEC010000277          283 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.291, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000277 DARBEC010000000
VERSION     DARBEC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 283)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 283)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..283
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.291"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            1..283
                     /locus_tag="SCL80_002961"
                     /pseudo
     CDS             1..283
                     /locus_tag="SCL80_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          109 a           57 c           56 g           61 t
ORIGIN      
        1 aacaaatcgt cgatctctat ctcgctggta agcctcgcgc agaaattatt cgagagtatg
       61 agcttacgcc ttcttctttc gataaatgga tgaagcaagc acaatcaacg ggctcattca
      121 aagaaagaga caacttaaca ccagaacaag cagaattgat cgcactaaga aagaaaaata
      181 agcaactcga aatggagaat gatattttaa agcaagcggc gctgatattc ggacgaaaag
      241 acaagtaatt gatgccaaca agcataaata ttccatatca gcg
//
LOCUS       DARBEC010000278          270 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.292, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000278 DARBEC010000000
VERSION     DARBEC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 270)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 270)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..270
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.292"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>270
                     /locus_tag="SCL80_002962"
     CDS             <1..>270
                     /locus_tag="SCL80_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEG1108748.1"
                     /translation="IHRRFYTSICHQKITTDTTEFKYHEVDAKGIIRQKKLYLDPFMD
                     MFNSEILSYRISEKPNALAVMEGLEEAIQTTNDCPYRRTFHSDQGW"
BASE COUNT           83 a           56 c           53 g           78 t
ORIGIN      
        1 atccatcgtc gtttctacac cagtatctgc catcagaaaa tcacgacgga tacaactgaa
       61 tttaaatacc atgaagtgga tgccaaaggc attatccgtc aaaagaaatt gtatctagat
      121 ccttttatgg atatgttcaa tagtgagatt ttatcctatc gtatctctga aaagcccaat
      181 gcattagcgg taatggaagg tttggaggaa gctatccaga ccactaacga ttgtccctat
      241 cgtcgtacgt ttcactcaga tcaaggttgg
//
LOCUS       DARBEC010000279          269 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.293, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000279 DARBEC010000000
VERSION     DARBEC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..269
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.293"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT          113 a           28 c           49 g           79 t
ORIGIN      
        1 ttaaggtgtc actaaaaatt ataaaaaata gtgttgacgc acttttgatg tcatgttata
       61 ataacgaagt cgtttggatg agatagctaa taaagcttga ttttatcgaa aaaaacttcg
      121 aaaaagttat tgacaaataa caataaagat ggtatgataa tcaagtcgct gctaatgagt
      181 ggcaaagacg tttagaaaaa ataaacaaag aagttgttga cattctcttc tgaatctgat
      241 aagattaaag agttgttaaa acaaaaaga
//
LOCUS       DARBEC010000280          255 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.294, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000280 DARBEC010000000
VERSION     DARBEC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 255)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 255)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..255
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.294"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           79 a           46 c           39 g           91 t
ORIGIN      
        1 aattggagtg gtagttaatt agtgacttat ttttgatttt atagcactga tactataaaa
       61 tatagatatc atcatattac acaatcttaa tactaactta aaaaatatct cctttcacaa
      121 gtatggtgat aaaattcgtt ccgggctacc tttttaggta gcctacttta atctttatac
      181 ctttctggat caacgaaagt atactttata tagtcataac gccgatgatc gctacgtgcg
      241 tccggcacgt cagtc
//
LOCUS       DARBEC010000281          240 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.296, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000281 DARBEC010000000
VERSION     DARBEC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.296"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>240
                     /locus_tag="SCL80_002963"
     CDS             <1..>240
                     /locus_tag="SCL80_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1108749.1"
                     /translation="IWLRKKRDTQAAYAFLKRLVKQFDEPKVVVTDKAPSITSAFKKL
                     KEYGFYQGTEHRTIKYLNNLIEQDHRPVKRRNKFYR"
BASE COUNT           86 a           46 c           46 g           62 t
ORIGIN      
        1 tatttggtta cgtaaaaaac gggacacaca agcagcctat gcttttctta agcggttagt
       61 gaagcagttt gatgaaccga aggttgtagt cacagataaa gccccctcta ttacaagtgc
      121 ctttaagaaa ctaaaagaat acggctttta tcaagggaca gaacatcgta ccattaaata
      181 cctgaataat ttgattgaac aagaccatcg tccagtaaag agacgcaata aattctatcg
//
LOCUS       DARBEC010000282          240 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.297, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000282 DARBEC010000000
VERSION     DARBEC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 240)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 240)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..240
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.297"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            80..>240
                     /locus_tag="SCL80_002964"
     CDS             80..>240
                     /locus_tag="SCL80_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1108750.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT           92 a           44 c           36 g           68 t
ORIGIN      
        1 ttaagctaga caactaaaac agtcatttgt gctacactca aaatagttcc gaccaaagaa
       61 ttataaggag tgagtgcaaa taacctatac ctatcttaca acggatgaac ttgtaataat
      121 agagtcttat ttgaaaatta accaatctgt tgctaaaact gcgcattgct tgaatcgttc
      181 aagtcaaacg atccataaag tatatctgtt cttcaagcaa agaaaatcag ccttagaata
//
LOCUS       DARBEC010000283          239 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.298, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000283 DARBEC010000000
VERSION     DARBEC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 239)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 239)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..239
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.298"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>239
                     /locus_tag="SCL80_002965"
     CDS             <1..>239
                     /locus_tag="SCL80_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1108751.1"
                     /translation="AIIALKPEGRKAVDIENSINEWLQSVPKNLFKSITFDCGKEFSN
                     WKSISNTNDIDIYFADPGTPSQRGLNEHSNGLLRK"
BASE COUNT           84 a           46 c           43 g           66 t
ORIGIN      
        1 gccattatcg ccttgaaacc agaaggccgt aaggcagttg atattgaaaa ttcgattaat
       61 gaatggcttc aatccgtacc caaaaatctt ttcaaatcaa ttacctttga ttgtggaaaa
      121 gaattctcta attggaaaag tattagtaat acgaatgata ttgatattta tttcgcagac
      181 ccaggaacgc cttcccaacg gggattaaat gaacattcaa acggactcct ccgaaaaga
//
LOCUS       DARBEC010000284          233 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.299, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000284 DARBEC010000000
VERSION     DARBEC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 233)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 233)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..233
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.299"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>233
                     /locus_tag="SCL80_002966"
     CDS             <1..>233
                     /locus_tag="SCL80_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_064305258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS6 family transposase"
                     /protein_id="HEG1108752.1"
                     /translation="QQDVIIVAVGYYLRYNLSYREVQEILYDRGINVSHTTIYRWVQE
                     YGKLLYQIWKKKNKKSFYSWKMDETYIKIKGKW"
BASE COUNT           83 a           35 c           45 g           70 t
ORIGIN      
        1 tcagcaggat gtgattattg tagccgtggg ctactatctt cgttataacc ttagctatcg
       61 tgaagttcaa gaaatcttat atgatcgtgg cattaacgtt tctcatacga cgatttatcg
      121 ttgggtgcaa gaatatggca aactactcta tcaaatttgg aaaaagaaaa ataaaaaatc
      181 cttttattca tggaaaatgg atgaaacgta catcaaaatt aaaggaaaat ggc
//
LOCUS       DARBEC010000285          227 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.300, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000285 DARBEC010000000
VERSION     DARBEC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 227)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 227)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..227
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.300"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..>227)
                     /locus_tag="SCL80_002967"
     rRNA            complement(<1..>227)
                     /locus_tag="SCL80_002967"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT           48 a           77 c           45 g           57 t
ORIGIN      
        1 atcgtctgca ttccttagcc tcctgcgtcc ctccattgct caaacaaaaa caacgggtac
       61 aggaatatca acctgttatc catcgcctac gcctgtcggc ctcggcttag gtcccgacta
      121 accctgggcg gacgagcctt ccccaggaaa ccttagtcat tcggtggaca ggattctcac
      181 ctgtctttcg ctactcatac cggcattctc acttctaagc gctccag
//
LOCUS       DARBEC010000286          224 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.301, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000286 DARBEC010000000
VERSION     DARBEC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 224)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 224)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..224
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.301"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>224
                     /locus_tag="SCL80_002968"
     CDS             <1..>224
                     /locus_tag="SCL80_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HEG1108753.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHEDNEGDSGVAA
                     ESQTPAKPAAEPTVDTESAGSSSEGVFQFK"
BASE COUNT           70 a           47 c           58 g           49 t
ORIGIN      
        1 ccgtcacctg ttacaggaac ggttaaaaat gtgattgttc cagaaggtac cgtagcaaat
       61 gttggggatg tattagtaga aatcgatgca ccaggacacg aagacaatga aggggacagt
      121 ggtgtagccg cagaatccca aactccagct aaaccggctg ctgaacccac tgttgataca
      181 gaatcagctg gatcgtcaag cgaaggagtc ttccaattta agtt
//
LOCUS       DARBEC010000287          223 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.302, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000287 DARBEC010000000
VERSION     DARBEC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 223)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 223)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..223
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.302"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
BASE COUNT           91 a           36 c           28 g           68 t
ORIGIN      
        1 attgtgatcc acctcttttg tttattagtt taaaccatca aaccattttt aataaaatat
       61 gtaaattcct ttgctcaaaa aagtagcttc tttacgattc gacaatttca aataaagcag
      121 gtaaaaactg gctgtcagaa aatagccaaa ctgaagcaaa aaataatgtt aaaattaatt
      181 aatgaacgaa aaaaatccct cacctaatat tacggtgagg aat
//
LOCUS       DARBEC010000288          211 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.303, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000288 DARBEC010000000
VERSION     DARBEC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..211
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.303"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(<1..194)
                     /locus_tag="SCL80_002969"
     CDS             complement(<1..194)
                     /locus_tag="SCL80_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEG1108754.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRR"
BASE COUNT           54 a           39 c           40 g           78 t
ORIGIN      
        1 caacgtcgct tattttcttt gtattgttca aagtattcaa gtgctgttcc accacatttg
       61 agaaagttat agacattgta aattgtttgg cgtccacgtt taagctgctt cgcaacgata
      121 gcaaccggag tttcttgatg aaaatatgct tctatcatta caagctcgtt tggtgtaaga
      181 tgggtataag tcatttatgt tcactctcct t
//
LOCUS       DARBEC010000289          205 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.305, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000289 DARBEC010000000
VERSION     DARBEC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 205)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 205)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..205
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.305"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>205
                     /locus_tag="SCL80_002970"
     CDS             <1..>205
                     /locus_tag="SCL80_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108755.1"
                     /translation="LVMDMNASYCQLLKTVFPNAEIVTDRFHIVQHINRSFNQLRVQI
                     MNRFRTSHSENQKKYRRLKRYWKL"
BASE COUNT           62 a           49 c           38 g           56 t
ORIGIN      
        1 ctagtgatgg acatgaatgc cagctattgt cagttgctta aaacagtctt tccaaatgcg
       61 gagatcgtaa ctgaccgctt tcatattgtc caacacatca atcgcagctt caatcaatta
      121 cgtgtccaga tcatgaatcg ttttcgaacc tcacactctg aaaatcagaa gaagtaccgt
      181 cggctcaagc gatattggaa actct
//
LOCUS       DARBEC010000290          203 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.denovo.306, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000290 DARBEC010000000
VERSION     DARBEC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 203)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 203)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..203
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.denovo.306"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..>203
                     /locus_tag="SCL80_002971"
     CDS             <1..>203
                     /locus_tag="SCL80_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108756.1"
                     /translation="FEDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLE
                     CLNNHIKVLKRNAYGFRNFYNFK"
BASE COUNT           71 a           46 c           31 g           55 t
ORIGIN      
        1 tatttgagga ttgtttaact agagacatta gcggtctgcc agaaacctat acaaccactt
       61 tacgaacgtt taaaaagttt ctgccacaga tccaaaatgc gcttcactac agctattcaa
      121 acggaccatt ggaatgctta aacaaccaca tcaaagtatt aaaacgcaat gcgtacggct
      181 ttcgcaactt ttacaatttc aaa
//
LOCUS       DARBEC010000291        19645 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.055, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000291 DARBEC010000000
VERSION     DARBEC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19645)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19645)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19645
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.055"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            253..567
                     /locus_tag="SCL80_002972"
     CDS             253..567
                     /locus_tag="SCL80_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000421280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1108757.1"
                     /translation="MELKYVIPNMEKTFGQLEYAGEGNIEQRRINGRPTILSRSYNLY
                     STIQRADDIVVILPSEAGEKHFESDEKVKLINPHITAEGYKIGNRGFTNYIMHADDMV
                     KA"
     gene            588..965
                     /locus_tag="SCL80_002973"
     CDS             588..965
                     /locus_tag="SCL80_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001234189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEG1108758.1"
                     /translation="MRLAEGIVIEKDQTFGTLKFSALRREVRIQNEDGTVSEEIKERT
                     YDLKSKGQGRMIQVSIPASVPLKEFDYNAEVELINPVADTVATATFRGADVDWYVKAD
                     DLVLKGQTKPHQKYKKEASESEK"
     gene            975..1748
                     /locus_tag="SCL80_002974"
     CDS             975..1748
                     /locus_tag="SCL80_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002388713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108759.1"
                     /translation="MTNTPLILKEIPKDEAISFIRQYHYSKILPRLCKYFLGIFSEEK
                     LLGVVELGWGTQPLQTIRKLFPDSSLQTTDYLEIGKMCFLPEMNQTNYFGSQALSALI
                     KWLKEHTDCHFLYTLADGIEGKCGYVYQASNFFYCGYFKTSVYRDKQSWEKIHPRSAR
                     LLLEENARFEQVEKKHWLSQAFCEYKGIEKINGRMFRYLYPLTKEAKKLLGHTLYRRH
                     YYPKEKNLRFEKRIAYQKYEAISQPTFDKQARIYNTQLF"
     gene            1770..3173
                     /locus_tag="SCL80_002975"
     CDS             1770..3173
                     /locus_tag="SCL80_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001130245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEG1108760.1"
                     /translation="MPLLHDRGERIKANDKHLVYHFVLISLLVVFLVVLLPFHWRALQ
                     SVNWQAVQLDTFFYQLHTPYFWSSILTALFVCGLISWLYYHYRIDSIKQLEHRQKLAR
                     MILENHWYEAEETQSEGFFKDLPSKKTKQRIRHFPKIYYRMKDGLIHIHVEITLGKYQ
                     DQLLHLENKLETGLYCELVEKTLKDSYVEYVLLYDTIGNRIGIEDVVAQDGRVKLMES
                     VYWEFDSLPHMLIAGGTGSGKSYFILTLIESLLHTNAKLYILDPKNADLADLGAVMNN
                     VYYRKEDMLACINQFYEDMIDRSETMKQHPNYKTGENYAYLGLSANFLIFDEYVAMMD
                     MLGRESTTVIHKLKQIVMLGRQAGFFLILACQRPDAKYFSDGIRDNFNFRVALGRMSE
                     MGFGMMFGSDTQKQFFLKPIKGRGYVDTGKSVISEFYTPLVPKRYDFLGEIGKVIQKK
                     QSEPVPHEVKGTGSDLL"
     gene            3355..4539
                     /locus_tag="SCL80_002976"
     CDS             3355..4539
                     /locus_tag="SCL80_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000426690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEG1108761.1"
                     /translation="MENEKIWIKELKEKRLAYGVSQNKLAVASHITRPYLSDIETGKA
                     VPTQQVKEDLLNALERFNPDNPLEMLFDYVRIRFPTNDVAQIIGEVLRLNMDYMLHED
                     FGYYSYPEHYRFGDIVVLVSHDVSKGVLLELKGKGCRQFENFLLAQHRSWYDFFQDCM
                     EHKGIFKRLDLAINDKTGILNIPDLAKKCKQEECISVFRSFKNYRSGELVHRDEKPYM
                     GNTLYIGSLKSEVYFCIYEKDYEQYVKNDVPLEDAEVKNRFEIRLKNDRATQAMKDLL
                     AHQQAEKTAFEIINRYIRFADKDDTKRRSDWKTNERWEWFIGKNRGALRLTTQPEPYS
                     FERTLNWLHHQVAPTLKIASILDVLNGTTIISTMIQEAKLTEKHEKLIEQQHLAMEDL
                     IT"
     gene            4536..4826
                     /locus_tag="SCL80_002977"
     CDS             4536..4826
                     /locus_tag="SCL80_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000055367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108762.1"
                     /translation="MSLLTKELKKLGFQCGIEFQAYIQNTGKYTSLIIEGKRQAGDTI
                     YTYDFYKVRFYNNYTNRVTVYGEHLTPFQLLRRVKSYIYYREKYLKERRTIT"
     gene            4823..5044
                     /locus_tag="SCL80_002978"
     CDS             4823..5044
                     /locus_tag="SCL80_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108763.1"
                     /translation="MNFGQNLYNWFLDNAQSLVLLAIVVIGLFLGFKREFSKLIGFLI
                     IALIAVGLVFNAAGVKDVLLNLFNRIIGA"
     gene            5086..5865
                     /locus_tag="SCL80_002979"
     CDS             5086..5865
                     /locus_tag="SCL80_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000678782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="abortive infection family protein"
                     /protein_id="HEG1108764.1"
                     /translation="MKGFMNMFTNDQFKSLLIKTLERKSSSNYYEGGNEIVSKMKISE
                     VTLDETDDFTYYKGYKKGWNTLCTYLKIRVPFDDLDFFESHKDLITQTASSIYDKQGD
                     NVLVDTILVPLPENYEVINFSQLKISDVVSQAIEDAESFMSNGEYQRAFDRVHTAFHG
                     YLIEILKKYEITVPRDENLSKLYSRIQQLIEKEIQPTELADIVKTTIRSSNGMISSLN
                     EARNRHSLAHPNTNIIGKREAKLIIGISSTVTDYISGYLDK"
     gene            5932..6426
                     /locus_tag="SCL80_002980"
     CDS             5932..6426
                     /locus_tag="SCL80_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000870467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEG1108765.1"
                     /translation="MKVYVANLGRYNEGELVGAWFTPPIDEEEMAERIGLNEDYEEYA
                     IHDFELPFDVDEYTPISEINRLCEAIQEIEGTPIYNELKEIQGMWFSSLEELLENKED
                     IHCYSDCDSMEDVARYYVEETGQLGEVPSNLQNYIDYQSLGRDMEIEGNYLVTSHGVF
                     EYCQ"
     gene            6482..7120
                     /locus_tag="SCL80_002981"
     CDS             6482..7120
                     /locus_tag="SCL80_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108766.1"
                     /translation="MVNVFENLKLLKLDMKDKGWVIDSFYFRYKQQNYIVLVKLFEKE
                     EKVPEYALLKLEFLKENDFSDMLAVYANSVKLYTDTKTIREYFGIEYSSNLGDVLFQF
                     SQTLARFIPTEVSEKKNEDQKEAMCFSLSQSDSEDPRKKYCFSVRRNPLKGNGELGKR
                     SPFNDNKTRLYRPSLYERLGSDTNLSFRYSMNPDDEETDEGIIAKWTKNKIE"
     gene            7192..7587
                     /locus_tag="SCL80_002982"
     CDS             7192..7587
                     /locus_tag="SCL80_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108767.1"
                     /translation="MKKIRSYTSIWSVEKVIYAINDLQLPFPITFTQMTWFIVSLFVV
                     ILFGSLPPLSLIDGALLKYLGIPIVLTGFMCQKTFDGKKPYGFLKSIITYALRPKVTY
                     AGKQIRLKKETMNESITIVRSEQLDGISD"
     gene            7571..10024
                     /locus_tag="SCL80_002983"
     CDS             7571..10024
                     /locus_tag="SCL80_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEG1108768.1"
                     /translation="MAYPIKYMEDNLVFNHDGEVFAYYELIPYNYSFLSSDEKIQVHD
                     NFCQLIAQNRDGKIHALQLATESSIRATQEQSKKEITGRLKEIAYEKIDEQTEALISM
                     IGDNQVDYRFFIGFKLYLNEQEVSVKNVGKEIQKAFAEFFHGVNHKLMGDFVSMPMAE
                     IERFSKMEQLLESKLSRRFQIRKLEKDDFGYIIEHLYGQTGVPFDDYDYHLPKRYEDK
                     KVLVKKYDILKPTRCLIEENQRYLRIEQEDTTTYVAYFTINSIVGELDFPNSEIFYYQ
                     QQQFTFPVDTSMNVEIVANKKALSTVRNKKKELKDLDSHAWESDNETSTNVVDALESV
                     DELESNLDQTKESMYKLSYVVRVSAKNLDELKQRCDEVKDFYDDLSIKLVRPFGDMLG
                     LHNEFIPSSKRYINDYIQYVTSDFLAGLGFGATQMIGENSGIYVGYNLDTGRNIYLQP
                     SLASQGIKGSVTNALASAFIGSLGGGKSFSNNMLVYYSVLFGGQATIVDPKAERGEWK
                     ETLPDIAHEINIVNLTSENDNKGLLDPYVILKLPKDSESLAIDILTFLTGISSRDSEK
                     FPVLRKAIRSVTQSKQRGLLLVIDELRKEDTPISNSIADHIESFVDYDFAHLLFSDGS
                     VTQSISLEKQLNIIQVADLVLPDSNSSFEEYTTMELLSVAMLIVISTFALDFIHSDRS
                     IFKIVDLDEAWSFLQVAQGKALSMRLVRAGRAMNAGVYFVTQNADDLLDEKMKNNIGL
                     KFAFRSTDLVEIKKTLEFFGVDKEDENNQKRLRELENGQCLVSDIYGRVGVMQFHPIF
                     EELLHAFDTRPPVRNEVEE"
     gene            10021..12048
                     /locus_tag="SCL80_002984"
     CDS             10021..12048
                     /locus_tag="SCL80_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000192391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108769.1"
                     /translation="MTQKQKKFLHYSWITLLCVGVILLLLGTLGNAVQATGLVDETID
                     TSNEYSKYGLNQYQLDYYVDNSWGWLPWNWSDGIGQSVMYGLYAITNFIWTISLYLSN
                     ATGYLVKEAYALDFISQTSQAIGENMQTIAGITPNGFSSSGFYVGFLLLFILIVGIYV
                     AYTGLVKRETTKVIRAVLNFVVVFILSASFIAYAPDYIAKINDFSKDVSTASLNIGTK
                     IVLPNTDSQGKDSVDMIRDSLFSVQVKQPWLLLQYGSTDVEALGEERVESLLSTSPDT
                     NNGEDRENVVIEEIEDHDNTYLTLPKTISRLGTVFFLFVFNIGISIFVFLLTGIMIFS
                     QVLFIIFAMFLPISFLLSMLPSFDGMGRHAITKLFNVIMTRAGITLIITVAFSISTML
                     YSLSASSPFFMIMFLQIVTFAGIYFKLGDLMSLFTLQSNDSQNMGRQVFRRPRQMMNR
                     QTRRFQRSMSKIFRGKAGSTKQKTMTTKQNQTNKANHTRQNERTTPKKETALSNVKKQ
                     QGGIGQRLGEKTAHVLDTKNRLVNKRKQAKQQIQATPTHVKYALHQGKENVTHNVTDF
                     KQSMADTKAQRKQQRMNQQEQKRKNIAQKRLELDKAKEKRRKENPSSPVQNNQQHVRK
                     RQQTVTPVLSQNTKRENHQRPLKKEQKQKTPPKQFNQRKLNKRTDKRGQQT"
     gene            12045..13067
                     /locus_tag="SCL80_002985"
     CDS             12045..13067
                     /locus_tag="SCL80_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000768374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEG1108770.1"
                     /translation="MKLKITVICSVVFSLFFFLLLFLVIFFADDTDSGENNKDSSIPQ
                     GGVTVSPEVLAHRPLIEKYGKEYGIEDYVSYILAIMQVESGGTAEDVMQSSESLGLPP
                     NSLSTEESIKQGVKYFSELLTSAEQQGVDIDSVIQSYNYGGGFLNYVRSHGKKYTYEL
                     AEQFSKEKSGGQKADYPNPIAIPVNGGWRYNYGNQFYVQLVSQYLTDTSPTEFDDETV
                     QVIMDEALKYEGFPYVFGGASPTTSFDCSGLIQWVYDKAGISLPRVAQDQYDATQEIS
                     MEEAQAGDLIFFHSTYNAGTYVTHVAIYLEGNRFYHAGDPIGYGDLSSRYWQDHLIGA
                     RRVIHN"
     gene            13084..14013
                     /locus_tag="SCL80_002986"
     CDS             13084..14013
                     /locus_tag="SCL80_002986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017650613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEG1108771.1"
                     /translation="MIIKIERKEKKEKPKKSKKPSKQRKVPMIKVGTHKKLTFVLWVL
                     LIGSVGFGIYKNFTAIDTHTVRETEIIKQQIVDTNQVESFVKSFAKDYFSWQQSQEAI
                     DKRNEKLTHYLTEELQVLNEEMIRKDIPTSSSVNDIQVWQVSQVNENTFEVLFSVEQV
                     ITEDKDKETISSSFHVVVHIDESDNMVIIKNPTMSKKPQKSDYQPKQLESDHTVDTET
                     MDEITSFLETFFQLYPTATEKELTYYVSNHVLPMINKEYVFEELVNPIFTRKDNQVIV
                     NVAVKYLDQETKATQISQFELILEKQDNWKIVK"
     gene            14258..14374
                     /locus_tag="SCL80_002987"
     CDS             14258..14374
                     /locus_tag="SCL80_002987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEG1108772.1"
                     /translation="MILKYPENICMLCIPMVMHKNPSDKSIYHWDFYALLGF"
     gene            14390..15193
                     /locus_tag="SCL80_002988"
     CDS             14390..15193
                     /locus_tag="SCL80_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEG1108773.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLFPLYH
                     GSAKSNIGIDNLIEVITNKFYSSTHRGPSELCGNVFKIEYTKKRQRLAY"
     gene            15428..15664
                     /locus_tag="SCL80_002989"
     CDS             15428..15664
                     /locus_tag="SCL80_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108774.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            15671..17623
                     /locus_tag="SCL80_002990"
     CDS             15671..17623
                     /locus_tag="SCL80_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEG1108775.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(17695..18192)
                     /gene="dfrG"
                     /locus_tag="SCL80_002991"
     CDS             complement(17695..18192)
                     /gene="dfrG"
                     /locus_tag="SCL80_002991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEG1108776.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            18554..19594
                     /locus_tag="SCL80_002992"
     CDS             18554..19594
                     /locus_tag="SCL80_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000288739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEG1108777.1"
                     /translation="MYTSINGELCKIDRAYSGEIVILQNEFLKLNSVLGDTKLLPQRK
                     KIENPHPLLQTTVEPSKPEQREMLLDALLEISDSDPLLRYYVDSTTHEIILSFLGKVQ
                     MEVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVSPLP
                     LGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYSPVS
                     TPADFRMLTPIVLEQAFRKAGTELLEPYLSFKVYAPQEYLSRAYNDAPKYCANIVNTQ
                     LKNNEVIIIGEIPARCIQDYRNDLTFFTNGLSVCLAELKGYQVTTGEPVCQTRRLNSR
                     IDKVRYMFNKIT"
BASE COUNT         6710 a         3265 c         3822 g         5848 t
ORIGIN      
        1 tgctttatcc cactcaatta ttagataaaa tagaatttga ttataatatt taaaatacct
       61 aggtagtcgt tgttttaaat atagtaggaa tcggacgctc agtacggagc gtctattttt
      121 ttacccaatt ttgaaaggaa gtgaacttat gaaaataaaa aatcaagaat caaaaggtcg
      181 ctctcccccc tttaaaacca tcacacaaat aagattcatt caaacataaa aacagaaagg
      241 ataggtaaaa atatggaact taaatatgtc attcccaata tggaaaaaac atttggacaa
      301 ttagaatatg ctggtgaagg gaacattgaa caacgtcgaa taaatggcag accaaccatt
      361 ctctctcgta gttacaattt atactcaacg attcaacgag cagatgatat tgttgtcatt
      421 cttccctccg aagctggcga gaaacatttt gaatccgacg aaaaagtcaa actcatcaac
      481 ccacatatta ccgcagaagg ttataaaatt ggcaatcgag gatttaccaa ttacattatg
      541 cacgcagacg atatggtgaa agcataagaa aggaaaggtg aaaaaatatg agattagcag
      601 aaggtattgt gattgaaaaa gaccagacat ttggcacatt gaaattttcc gccttgcgtc
      661 gtgaggttcg tatccagaat gaagatggta cggtttccga agaaattaaa gaacgtacct
      721 atgacttaaa atcaaaagga caaggacgca tgatacaagt cagtatcccc gcttctgttc
      781 ctttgaaaga atttgactac aacgcagaag tggaattaat caatcctgtg gcagataccg
      841 tagcgacggc tacttttcga ggggcagacg ttgattggta cgtgaaagca gacgatcttg
      901 tattaaaagg acagacaaaa cctcatcaga aatataaaaa agaagcgtcc gaaagtgaaa
      961 aataggtgtt gcttatgaca aacacaccat tgattttgaa agaaattccg aaggatgaag
     1021 ccatttcttt tattcggcaa taccattatt caaagatact ccctagattg tgtaaatact
     1081 ttctagggat tttttcagaa gaaaaattat taggcgttgt ggaattaggc tggggtacgc
     1141 aacccctaca aaccatacgc aaactctttc ccgacagttc ccttcaaacc acagattatt
     1201 tagaaattgg aaagatgtgt tttcttccag aaatgaatca aaccaattat tttggcagtc
     1261 aagctctttc agctctaatt aagtggttga aagaacatac ggactgtcat tttttatata
     1321 cactggcaga tggaattgag ggaaaatgtg gctatgtcta tcaagcgtcc aacttctttt
     1381 actgcgggta tttcaagaca agtgtctatc gtgataagca gtcatgggaa aagatacacc
     1441 ctcgaagtgc tcgcctttta ttagaggaaa atgctcgctt tgaacaggta gagaaaaagc
     1501 attggctttc ccaggcattt tgcgaataca aagggattga gaaaatcaat ggacgcatgt
     1561 ttcgctatct ctatccgttg acaaaagaag cgaaaaaact gttaggacac actctctatc
     1621 gacgtcatta ttatccgaaa gaaaaaaatt tacgctttga aaagcgaatc gcttatcaga
     1681 aatatgaagc catttctcaa ccaacttttg ataaacaggc tcgtatctac aatacacaat
     1741 tattttgata ggtggaaagg aggattttta tgccattgct tcatgacaga ggagagcgaa
     1801 tcaaagccaa cgataagcac ttggtctatc attttgtact aatttcattg ctggtagttt
     1861 ttctagtcgt cctactccct tttcattgga gagcgttaca atcggtgaac tggcaagcag
     1921 ttcagttaga tacatttttc tatcaacttc atacgccata cttttggagc agtattctca
     1981 ctgctctttt tgtatgcgga cttatcagct ggctttatta tcactatcgt atagattcta
     2041 taaaacagtt agagcaccgt caaaagttag ctcgtatgat tttagaaaat cattggtatg
     2101 aagccgaaga aactcaatca gagggattct ttaaagattt acccagcaag aaaaccaaac
     2161 aacgaatccg ccattttcct aaaatatatt atcgaatgaa agatggacta atccatattc
     2221 atgtagaaat caccttaggg aaatatcaag atcagctttt gcacctagaa aacaaactag
     2281 aaacaggatt gtattgtgaa ttagtggaaa aaacgttgaa agattcttat gtagagtatg
     2341 tcctacttta tgatactatt ggcaatcgaa ttggcattga agacgtggta gctcaagatg
     2401 gacgtgtcaa attgatggaa tcggtctatt gggagttcga ttctctccct cacatgttaa
     2461 ttgctggggg cactggtagt ggaaaatcct attttattct cacattgatt gaatctctcc
     2521 tgcatacaaa tgcaaagctt tatattcttg accctaaaaa tgctgatttg gcagacttag
     2581 gggcggtcat gaataatgtc tattaccgta aagaagatat gttggcatgt atcaatcaat
     2641 tttacgaaga tatgattgat cgcagtgaaa ccatgaaaca acaccctaac tataaaacag
     2701 gagaaaatta tgcttatctt ggtttgtctg ccaattttct tatttttgat gaatatgtgg
     2761 caatgatgga tatgctaggt cgtgaaagta ccaccgttat tcataaactc aaacaaattg
     2821 taatgttagg acgacaagca ggcttctttt taattcttgc ctgtcaacgt ccagacgcaa
     2881 aatattttag tgatgggatt cgggacaact ttaacttccg tgtggctttg ggtcgtatgt
     2941 ctgaaatggg ctttggtatg atgttcggaa gcgatacaca aaaacaattt ttcttaaaac
     3001 ccatcaaggg aagaggttac gtggatacag gaaaaagtgt gattagtgag ttttatacac
     3061 cccttgtacc aaaaaggtac gatttcttag gagaaatagg aaaagtcatt caaaaaaagc
     3121 aatctgagcc tgtaccgcac gaagtgaaag gtacaggttc agacttgctt tgattggtgt
     3181 ggcgttagcc acgccaatcc tttagccccc tctatctaac aggggggcac aaatacaact
     3241 gaaaacaggc aattctcatc aaccaatgtt gatggtacaa aggattgcgg aaaatatgaa
     3301 aggtgtaaac tcaagggtca tagtttacat cttttttaga ttggaggttt gcgaatggaa
     3361 aacgaaaaaa tttggattaa ggaattgaaa gaaaagcgac tggcttatgg cgtttctcaa
     3421 aataaactgg cggttgcttc tcatattacc agaccttacc tatcggatat tgaaacaggc
     3481 aaggcagtcc ctactcaaca ggtgaaagaa gatttactga acgctttgga acgcttcaat
     3541 cccgataacc cattggagat gttattcgac tatgtacgga ttcgattccc taccaatgat
     3601 gtggctcaaa ttatcggtga ggttttacgt ttgaatatgg attatatgct acacgaagac
     3661 tttggctatt attcttatcc cgaacactat cgtttcggtg atattgttgt cctagtctct
     3721 catgatgtaa gtaaaggtgt tttattagaa ctgaaaggaa aaggctgtcg gcaatttgaa
     3781 aactttctat tggctcaaca tcggagctgg tatgatttct ttcaagattg catggaacat
     3841 aaaggtattt tcaaacgctt ggatttagct atcaacgata aaacagggat tttgaatatt
     3901 ccagatttgg caaagaaatg taaacaggaa gaatgcattt ccgtctttcg aagctttaaa
     3961 aattatcgca gtggtgaatt ggttcaccgt gatgaaaaac catacatggg aaacacactt
     4021 tatattggct cattaaaaag tgaagtctat ttctgtatat atgagaaaga ttatgagcaa
     4081 tacgtgaaaa atgatgttcc cttagaagac gcagaagtaa agaatcggtt tgaaattcgg
     4141 ttaaaaaatg accgagccac ccaagccatg aaagatttat tggcacatca acaggcggaa
     4201 aaaacagcct ttgaaattat caatcgctat atccgctttg cagataaaga cgacactaaa
     4261 cgtcggagtg attggaaaac taatgaacgt tgggaatggt ttatcggtaa aaatcgtgga
     4321 gcattacgcc taaccacaca gccagagcct tattcttttg aacgaacatt gaactggtta
     4381 catcatcaag tcgcccctac attaaaaatc gcaagcattt tagatgttct caacggtaca
     4441 accattatct ccaccatgat tcaggaagcc aaactgacag aaaaacatga gaaattaatt
     4501 gaacaacaac atctagccat ggaggatttg attacatgag ccttttaaca aaagaattaa
     4561 agaagttagg gtttcagtgt ggcattgagt ttcaagcgta tatacaaaat acagggaaat
     4621 atacgtctct cattattgag ggaaaacggc aagctggtga taccatctat acctatgatt
     4681 tttacaaggt gagattttat aataattata ccaatcgagt aaccgtctat ggagagcatt
     4741 taaccccttt tcaattatta aggcgtgtaa aaagctatat ctattatcga gaaaaatatc
     4801 ttaaggaaag gaggacaatc acatgaattt tgggcagaat ttgtacaact ggttcttgga
     4861 taatgcacag tcattggtat tgttagcgat tgttgtcatt ggactgtttt taggttttaa
     4921 acgagaattt tcaaaactca ttggcttctt gattattgct ctaatcgccg tcggacttgt
     4981 gttcaatgca gctggcgtga aagatgtgtt attaaactta tttaaccgaa tcattggtgc
     5041 ttaacagata tttttcatga taaaatagga agtatatatt ttgttatgaa aggatttatg
     5101 aatatgttta caaatgatca atttaaatct cttttaatta aaacattaga aagaaagagc
     5161 tcttcaaatt attatgaagg aggtaacgag attgtttcta aaatgaaaat atcagaggta
     5221 actttagatg agactgacga ttttacttat tataaaggat ataaaaaggg ctggaatacg
     5281 ctttgcacat atcttaaaat aagagttcct tttgatgatt tagacttttt tgaaagtcat
     5341 aaagatttga ttacccagac agcaagctcc atatatgata aacaaggtga taatgtatta
     5401 gtggatacta ttttagtacc acttccagag aattacgaag taattaactt tagtcagttg
     5461 aaaattagtg atgtggtttc acaagctatt gaagacgctg agtcttttat gagtaatggt
     5521 gaatatcaac gtgcctttga cagagttcac actgcttttc atggatattt aattgaaata
     5581 ctaaaaaaat atgaaattac agttcctaga gacgaaaatc tatctaaatt atatagtagg
     5641 atacaacaat taattgaaaa agaaattcaa cctactgaac ttgctgatat agtgaagaca
     5701 acgataaggt catcaaatgg aatgattagc tctcttaatg aagcaagaaa taggcattca
     5761 cttgcacacc ctaatacaaa tattattgga aagagagaag caaaactaat aataggtatt
     5821 tcaagtacag ttacagatta tattagcgga taccttgata agtagcgtca ttaactgatt
     5881 gctacttttt ttatgactat ttttataaaa aggatgtgat aagaattgga aatgaaagtc
     5941 tatgtggcaa atttaggacg gtataatgaa ggtgaattag tcggagcatg gtttactcca
     6001 ccaattgatg aggaagaaat ggcagaaaga atcggtttaa atgaggacta cgaagaatat
     6061 gcaatacatg attttgagct tccttttgat gtggacgaat acacgccgat tagtgaaatc
     6121 aaccgtctat gtgaagccat tcaagaaatc gaaggtacac ctatttataa tgaactcaaa
     6181 gaaattcaag gcatgtggtt tagtagcttg gaagaattac tggaaaacaa agaggatatt
     6241 cattgttata gtgattgtga ttcgatggaa gatgtcgccc gttattacgt ggaagaaaca
     6301 ggacagcttg gtgaagtccc ttccaattta caaaattata ttgactacca atctttggga
     6361 cgtgatatgg aaatagaggg taattaccta gttacctctc atggggtatt tgaatattgt
     6421 cagtaagttt aacaatccgt gttataatta aattaataaa tagcacggag aggtataaat
     6481 tatggtaaat gtatttgaaa atcttaaact attaaaacta gacatgaaag ataaaggctg
     6541 ggtgatagat tccttttact ttcgttacaa acagcaaaat tatattgtac ttgttaagct
     6601 atttgaaaaa gaggaaaaag ttccagaata tgctctgtta aaactagaat ttttaaagga
     6661 aaacgatttt tcagatatgc tagctgtcta tgctaattcg gtaaaattat atacagacac
     6721 gaaaacaata agagaatatt ttggtatcga atacagtagt aacttgggag atgttttatt
     6781 tcaatttagc caaactcttg ctcgatttat tccgacagaa gttagtgaga agaaaaatga
     6841 agaccaaaaa gaagccatgt gtttctcatt aagtcaaagt gattcagaag atcccagaaa
     6901 aaaatattgt ttttcagtta gaagaaatcc tttaaaggga aatggtgagc tgggaaaacg
     6961 aagtcctttt aatgacaata aaacgagatt gtatagaccc agcttatatg aacgattagg
     7021 gtcagatacc aatttaagtt tcagatattc aatgaatcca gatgatgaag aaactgatga
     7081 aggtattatt gcaaaatgga caaaaaataa aattgaatag ttaaagcatt gaaagagcaa
     7141 tcacctattt tgattgttct ttttctattt ttacggaaag gacgtgaaga catgaaaaaa
     7201 ataagaagtt acaccagtat ttggtcagta gaaaaagtga tttatgcgat taatgattta
     7261 caactccctt tcccaattac ctttacgcaa atgacatggt ttatcgtttc tttgtttgtc
     7321 gtcatactct ttggcagttt accaccactt tctctgattg acggagcact actcaaatac
     7381 ttagggattc ccattgttct cactgggttc atgtgccaaa agacctttga cggaaagaaa
     7441 ccctatggat ttctaaagtc tatcattacc tatgctctac gccctaaagt aacctacgct
     7501 ggcaaacaaa tacgattaaa aaaagaaaca atgaatgaat cgattacgat tgtaaggagt
     7561 gaacaactag atggcatatc cgattaaata tatggaagat aatctcgtat ttaatcatga
     7621 tggcgaagta ttcgcttatt atgaattaat tccctataat tacagttttt taagttccga
     7681 tgaaaaaatt caagttcatg ataatttctg tcaattgatt gcccaaaatc gtgatggcaa
     7741 gattcatgct ctgcaactag caaccgaatc aagtattcga gccacacagg aacaatcgaa
     7801 aaaagaaatc actggacgat taaaagagat agcttacgaa aaaatagatg aacagaccga
     7861 agcgttaatt tctatgattg gtgacaatca agtagactat cgctttttta ttggttttaa
     7921 actctacctc aatgaacagg aagtatccgt aaaaaatgtt gggaaagaaa ttcaaaaagc
     7981 atttgcggaa ttctttcatg gtgtgaatca caaattaatg ggcgattttg tttctatgcc
     8041 tatggcggaa atcgaacgct tttctaagat ggaacagttg ttagaaagta aattatccag
     8101 acgctttcag attcgtaaat tggagaaaga tgattttggt tatattattg aacatcttta
     8161 cgggcaaaca ggtgtccctt ttgatgacta cgactaccat ttaccgaaac gatacgaaga
     8221 taaaaaagtg ttggtaaaga aatacgatat tctcaaaccc actcgctgtt taatcgaaga
     8281 aaatcaacgc tatttacgga ttgaacagga agataccact acttatgtgg cgtattttac
     8341 aattaatagt attgttggtg aattggattt ccctaatagt gaaatttttt attaccagca
     8401 acagcagttt actttccccg ttgataccag tatgaatgtt gaaattgtcg ccaataaaaa
     8461 agccttatct accgttcgca ataaaaagaa agaactgaaa gatttagata gccacgcatg
     8521 ggaaagcgac aacgaaacca gtactaatgt ggtggacgct ttagaatctg tcgatgaatt
     8581 agaaagtaat ttagaccaaa ccaaagaatc tatgtacaaa ctaagctatg tggtacgggt
     8641 atcagcaaag aatttggacg agctgaaaca acgttgtgat gaagtaaaag atttttatga
     8701 tgatctgtct atcaaattag tacgaccttt tggcgatatg ctaggcttgc ataatgaatt
     8761 tattccctca agcaagcgat atatcaatga ctatattcaa tatgtcacgt cggatttttt
     8821 ggctggtctt ggtttcggtg ctacacaaat gattggtgaa aattctggta tttacgtcgg
     8881 ttacaatctc gatacaggca gaaatattta tctccaacct agccttgcaa gtcaaggaat
     8941 taaaggctct gtaacaaatg cattagcgtc cgcttttatt ggttcattag gtggcggaaa
     9001 atcattctct aacaatatgc tggtctatta ttcagtactc tttggcggac aggcaaccat
     9061 tgttgaccca aaagctgaac gtggtgaatg gaaagaaacc ttacctgata ttgcccatga
     9121 aatcaatatt gttaatctca cgagtgagaa cgacaataaa gggttacttg acccttatgt
     9181 gattttaaaa ctcccgaaag attcagaaag ccttgccatt gatattctca cgttcttaac
     9241 aggtatttcc agccgagata gcgagaagtt tcctgtcctg cgtaaagcta ttcggagtgt
     9301 aactcaaagc aaacaacgag ggttgttatt ggtgattgat gaattgcgaa aagaagatac
     9361 gcccatcagc aatagtattg ctgaccatat cgaaagcttt gtcgattatg attttgccca
     9421 tctgttattc agtgatggca gtgtcaccca atccattagc ttggaaaaac aactcaatat
     9481 tattcaagtg gcagatttgg tcttacccga ttccaatagc tcttttgaag aatacactac
     9541 catggaatta ttaagtgtgg caatgctgat tgtcattagt acctttgctt tagactttat
     9601 ccactctgac cgttccattt ttaaaattgt tgaccttgat gaggcttgga gttttttaca
     9661 agtggcacaa ggaaaagctc tatctatgcg attggttcga gcaggacgtg ccatgaatgc
     9721 aggggtctat ttcgttactc aaaacgcgga cgatttacta gatgaaaaga tgaagaataa
     9781 tattggcttg aaatttgcct ttcgttccac tgacctagtg gaaatcaaaa agaccttaga
     9841 attttttggt gtggacaagg aagatgaaaa caatcagaaa cgcttacgtg aattagaaaa
     9901 tggacaatgt ttagtttcag acatctatgg tcgtgtaggg gttatgcagt ttcacccgat
     9961 ttttgaagag ttgcttcatg cctttgatac cagaccgcct gtgcgaaatg aggtggaaga
    10021 atgacacaaa aacaaaagaa atttcttcac tatagttgga taacgctcct ttgtgtgggc
    10081 gttattttac ttttattagg cacgctgggc aatgcagtac aagcgacagg cttagttgat
    10141 gaaaccattg atacaagcaa tgaatactcc aagtatggtt tgaatcaata tcagcttgat
    10201 tattatgtgg acaatagctg gggctggctt ccatggaatt ggagtgatgg cataggacaa
    10261 tcggtgatgt atggattgta tgccattact aattttattt ggacaatttc tctttatcta
    10321 tcgaacgcca caggttattt ggtgaaagaa gcctatgcct tagatttcat tagtcaaaca
    10381 tcccaagcaa ttggcgaaaa catgcagacg attgcaggca ttacaccgaa tggcttttcc
    10441 agttcagggt tttatgtcgg tttcctcctt ctttttattc tcattgttgg aatctatgtt
    10501 gcctatacag gattggttaa gcgagaaacg acgaaagtga tacgagctgt tttaaacttt
    10561 gtcgtggtgt ttattctatc ggcttccttt attgcgtatg cacccgatta tattgcgaaa
    10621 atcaatgatt tttccaaaga tgtcagtacc gccagtctga atattgggac aaaaattgtt
    10681 cttcctaaca cggatagtca agggaaagac agtgtggaca tgatacgtga cagcttattc
    10741 tctgttcaag taaagcaacc atggctattg cttcaatatg gctccactga tgtagaagcg
    10801 ttaggggaag aacgtgtgga aagtttattg tctaccagtc cagatacaaa caatggtgaa
    10861 gatagagaaa atgtagtgat tgaagaaatt gaagatcatg acaataccta tttaacccta
    10921 ccgaagacga tttccagatt aggaacagtc ttctttttgt ttgtattcaa tattggtatc
    10981 tcgatttttg ttttccttct aacaggaatc atgatttttt cgcaagtgct atttattatt
    11041 ttcgctatgt ttttgccaat cagtttccta ttaagtatgt tgccgagttt tgatggcatg
    11101 ggcaggcatg ccattaccaa gttatttaat gtgattatga caagggctgg tattaccttg
    11161 attattaccg ttgcctttag tatttccact atgctttatt cgctgtcagc aagttctccc
    11221 tttttcatga ttatgttttt acaaattgtg acctttgcag ggatttattt taaactgggc
    11281 gatttaatga gcctattcac tttacaaagc aacgactctc aaaacatggg cagacaagtg
    11341 tttcgcagac ctcgtcaaat gatgaatcga caaaccagaa gatttcaacg cagtatgagt
    11401 aagatttttc gtggaaaagc agggtcaacg aagcaaaaga caatgacaac aaagcagaat
    11461 caaaccaata aagctaacca tactcgtcaa aatgaacgga ctacaccaaa gaaagaaaca
    11521 gcactttcca atgtgaaaaa gcaacaaggc ggtatcgggc aacgattagg tgaaaagaca
    11581 gcccatgtat tggacacgaa aaatcgtcta gtcaataaaa gaaagcaggc aaaacaacag
    11641 atacaggcga caccaaccca tgtcaaatat gcccttcatc aaggaaaaga gaatgtcact
    11701 cataatgtaa cagactttaa acaatctatg gcagatacca aagctcaacg caaacaacag
    11761 cgtatgaacc agcaggagca aaaacgaaag aacattgctc aaaagcgttt ggagctggat
    11821 aaagcaaaag aaaaaagacg aaaagaaaac ccatcatctc ctgttcagaa taaccagcag
    11881 catgttcgca agcgtcagca aaccgtcacg cctgttttgt cacaaaacac taaacgtgaa
    11941 aatcatcagc gaccattgaa aaaagagcaa aaacaaaaga caccaccgaa gcaattcaat
    12001 cagaggaagc taaataaaag aacggataag cgaggacaac aaacatgaag ctaaaaataa
    12061 cagtgatttg tagtgtggtc ttctctctct tcttttttct cttattattc ctagtcattt
    12121 tctttgcaga tgatacggac agtggcgaaa acaacaagga ttcttctatt ccacaaggcg
    12181 gtgtgaccgt ttcaccagaa gtgttggcac accgtccgtt gattgaaaaa tatggtaagg
    12241 aatatgggat tgaagactat gtttcttata tcttagccat tatgcaagtg gaatcgggcg
    12301 gtacagcaga agatgttatg caaagttccg aatctttggg tttaccgccc aacagtttga
    12361 gtaccgaaga gtccatcaaa caaggggtta aatatttcag tgaattatta accagtgcag
    12421 aacaacaagg agtggatata gattctgtta tccaatctta taattatggc ggtggctttt
    12481 taaattatgt gaggagtcat gggaaaaaat atacctatga attagccgaa cagttttcta
    12541 aagagaagtc gggtggtcaa aaagcagact atcccaatcc tatagccata cctgtgaatg
    12601 gtggttggcg atataactat ggaaatcagt tttatgttca gttggtttca caatacctaa
    12661 ccgacacttc cccaacagaa tttgatgatg aaaccgtgca agtcattatg gacgaagcct
    12721 tgaaatacga agggtttccc tatgtctttg gaggggcgtc gcctaccacc tcttttgatt
    12781 gtagtggctt aatacaatgg gtgtatgaca aagctggcat ttctctccct cgtgtcgccc
    12841 aagatcagta tgacgctacg caagaaatct ctatggaaga agcccaagca ggcgacctta
    12901 tcttctttca ttcaacctat aatgcaggca catatgtgac tcatgtggcg atatatttag
    12961 agggaaatcg cttttatcat gcaggcgacc caatcggtta tggcgactta agcagtcgtt
    13021 attggcaaga ccatctgatt ggggctagac gtgttattca taactaagaa aggaatgaat
    13081 caaatgatta taaaaatcga gcgaaaagaa aagaaggaga aaccaaagaa atctaaaaaa
    13141 ccgagcaagc aaagaaaagt tccgatgatt aaagttggca cgcataagaa attaaccttt
    13201 gttttatggg ttttattgat tggtagtgtc ggatttggaa tctataaaaa ttttactgcc
    13261 attgacactc atactgtccg tgaaacagaa atcattaaac agcagattgt cgataccaac
    13321 caagtagaaa gttttgtgaa atcctttgcg aaagactact tttcgtggca acaatcccaa
    13381 gaagccattg ataaacgaaa tgaaaaattg acccattatt tgacggaaga attgcaggta
    13441 ttaaatgaag aaatgattcg taaggatatt cctactagtt ctagtgtgaa cgatatacag
    13501 gtgtggcagg tttctcaagt gaatgagaac acctttgagg tactgttttc tgttgaacaa
    13561 gttattaccg aagacaaaga caaggaaact atatcgtcta gctttcatgt ggttgtccat
    13621 atagatgaat cagataatat ggttattatc aaaaatccga caatgagtaa gaaacctcaa
    13681 aaatcggact accaacccaa acagctagaa agtgaccata cagtagatac agaaacgatg
    13741 gacgaaatca ctagcttttt agaaacgttc ttccagctct atcctaccgc cacagaaaaa
    13801 gaattgactt actatgtaag taatcatgtg ttacctatga ttaacaagga atatgttttc
    13861 gaggaattgg tgaatcctat ctttaccaga aaagataacc aagtaatcgt gaatgtcgct
    13921 gtgaaatatt tagatcaaga aacaaaggca acccaaatct cgcaatttga gttgatatta
    13981 gaaaaacaag ataattggaa gattgtaaaa taacaaatat tggtacatga ttacagatac
    14041 tttgtaatca tgtactcttt ttgataaaaa attggagatt cctttacaaa tatgctctta
    14101 cgtgctatta tttaagtgac tatttaaaag gagttaataa atatgcggca aggtattctt
    14161 aaataaactg tcaatttgat agcgggaaca aataattaga tgtccttttt taggagggct
    14221 tagttttttg tacccagttt aagaatacct ttatcatgtg attctaaagt atccagagaa
    14281 tatctgtatg ctttgtatac ctatggttat gcataaaaat cccagtgata aaagtattta
    14341 tcactgggat ttttatgccc ttttgggttt ttgaatggag gaaaatcaca tgaaaattat
    14401 taatattgga gttttagctc atgttgatgc aggaaaaact accttaacag aaagcttatt
    14461 atataacagt ggagcgatta cagaattagg aagcgtggac aaaggtacaa cgaggacgga
    14521 taatacgctt ttagaacgtc agagaggaat tacaattcag acaggaataa cctcttttca
    14581 gtgggaaaat acgaaggtga acatcataga cacgccagga catatggatt tcttagcaga
    14641 agtatatcgt tcattatcag ttttagatgg ggcaattcta ctgatttctg caaaagatgg
    14701 cgtacaagca caaactcgta tattatttca tgcacttagg aaaatgggga ttcccacaat
    14761 cttttttatc aataagattg accaaaatgg aattgattta tcaacggttt atcaggatat
    14821 taaagagaaa ctttctgccg aaattgtaat caaacagaag gtagaactgt atcctaatat
    14881 gtgtgtgacg aactttaccg aatctgaaca atgggatacg gtaatagagg gaaacgatga
    14941 ccttttagag aaatatatgt ccggtaaatc attagaagca ttggaactcg aacaagagga
    15001 aagcataaga tttcagaatt gttctctgtt ccctctttat catggaagtg caaaaagtaa
    15061 tatagggatt gataacctta tagaagttat tactaataaa ttttattcat caacacatcg
    15121 aggtccgtct gaactttgcg gaaatgtttt caaaattgaa tatacaaaaa aaagacaacg
    15181 tcttgcatat taagtagttc agttttggag tacaaaaagt gaaaatgtaa acctaaatga
    15241 gtatcaaatt gcaactgaat ttgatactcg ttttttattg ttaaatcaac gtttgttgtt
    15301 aatttacacc acaaaatgta agttaaaatg tatataaaaa tgcgaacgtg aaataattat
    15361 tgtaggaatg caaattggtg tgttaaaatg gttacaattt actaagcaaa aaggggtgca
    15421 ttttcctatg caatttcttg agccaaaaaa taagaacgct aagtctgtgg attgggaaat
    15481 atcagagcaa gtaagggtga tagttaagca atacgctgaa tatgcagagc gtactgaaag
    15541 cgaagcagtg gatgaattct tattgaacat tttagacgat aagaagttta ttgaatggat
    15601 tgccaacaag cgaagtaata agcggattgt tgaaaaaatg ggtatcaaag atagagtggg
    15661 ttgatagaca atgaccaagc ttaaaagatt agccacgaag gaagatgaaa ttgtagatgt
    15721 taaaattccg atttcaaacg aagaattaaa agatagggct aaacaatatg atttattaac
    15781 tccaaaacga tttgcaacaa ggtataacaa aatgttgttc ttgccaacgt cttttaaatg
    15841 gaacggttct gaatatccca ttcaatataa ttactgcatc aatccgtttt gttgtaattt
    15901 tgggaaagaa cagcacaaat tcaaagatgt aaaagggaaa ccaagtcgat ataaaatgac
    15961 tggtagttcc aaagataaag gtcataaagg tatgtactgc aatgacaatc ctattggcag
    16021 aggtgtatcg caaaactgta cagtaacccc tttatctaat tggtctgttg ttgaagaaat
    16081 taaacgcctt atagaaatca atagcatcca agatgttgag ccagattatc aatttcataa
    16141 agaaggctgc tcggaagaag aatcaacccc attcaatgag ccgaagcaat tttacaaaag
    16201 ggggaaaagt aggggcaaat ctcaacgcta tcaatgtaag gcgtgtaaga agttcactaa
    16261 tgttctacca aaaagagaag aaaccactac ttatcaccaa caaaagaaca ccattcttcc
    16321 aatgttcgct aagatggttg ttggaagggt ttcagtcagt cgtacttgtg atattttagg
    16381 aattggagtt ggaacctact atcataagct ggagtggctg tataggcgtt gtttggagtt
    16441 tttagaacga tatgagacac aaccccttca aacaaagaaa tttaacgaga tgtggcttaa
    16501 tacggacaag atgcactact acctaaataa cgtccgtaag aaaggacaag gttcaaagaa
    16561 atatacgggg tttgaagacc ttaatatgca aacgtatata gttgtatctg ctgaggtctt
    16621 atcaagatat gtgttccgtt ctgatgttgc ctatgattgg aatatttcaa tggatgaatt
    16681 aaatgaagat acaagaaaat tcaaagaaga ccatctcaat acttttagtc gtaaaaatga
    16741 taggttagat tggtcttatt atccacaaga accatccgct aacgatagcg aaaataggaa
    16801 tgcttatctt cacgaattag ggaaaattac taataggagt agatttgtcg atggtttaaa
    16861 tgttgatgct ccttatacta caactgctca ctattggtta attaaacaaa tggttaatgc
    16921 cgacgaatgg cgaatgatta gtgatgatga cttctctatt agaaacgcct tttatagagt
    16981 attcacgaaa gaattaaggt tgagcgatgc acatcatttt atttgccaag ttaacaagac
    17041 gaaaagcaga aaacaatgct taaaagaatt cggacaagca aaagcagaac tgctcgattg
    17101 gggagatatc agaggattca aaacaaagtt tctacggact ttggcatctc attatctaac
    17161 tgaattacta acaagtcatc aattccacga agaggctatt agtaaagatg gagaaagata
    17221 tcgcaaatat gccgataacc ctatcaaaca tccattagcc actaaggata aaggatttta
    17281 ttcagttgat tgtagaacgg atttatctgc tctggaacca aacgaaatcg caaagatgct
    17341 attaaatgtt aatgaccatt cgaccaatag ctttattcaa cagataagac gttatatatc
    17401 atctcttgaa agaccattaa caacagctcg tggtgataag aagagctata tttacgctaa
    17461 cttcaatccg aagtatgctc aatttgcgat tacaattcta agaacttact acaacttttg
    17521 tcgccctttt aaatcagcgg ataaaaaggt tttgacacct gctcaaagat tagggattac
    17581 agataaacaa tttgattgga aagatataat ttatttcaaa tgacaaaaag agcttggagt
    17641 aatcccaagc tcttgtgctt tatctttact gttcttcttc aattaacgca cccgttaact
    17701 caataagttt tttctttcat atacatgaaa atagtagtta tacggatttt tatcattctt
    17761 tatccctttt tgggcaaata cctcattcca ttcctcataa ttcacttctg gaaaaaaagt
    17821 atctccttcg aattcatgat gtatttttgt gatgtacatt ttctcaacat aagggaaaaa
    17881 caaattataa atctgttctc ctccgaaaat aaaaatttct tcttcgtttt tacataactc
    17941 aaaaacatct tctattgaat gaacaatttc acaaccatta aaggtaaacc ccttatctct
    18001 cgtcagaata atatttcttc tgtcaggtaa ggctcttccg attgattcaa ggttcttcct
    18061 acctaatatt atcggatgtc cctttgtagt atttttaaca tattcccagt ccttgggaat
    18121 cctccaagga atgtcattct ctttgccaat cactctattc ttatccatcg cagcaatcaa
    18181 agaaactttc atttataaca cagccttttt aactaattta ccatctaatt tcttatttct
    18241 atcaaaaaac cttttctcat tatcctgccc gttagttgaa taagcaattt tttctataag
    18301 ttatattatc ataataccac aaaatcacaa attttcattc aattatcatt ttcaattgca
    18361 ttttataaat cattttgtat gtaatttggt atgtaagtta aaatgaaaaa agtagtccat
    18421 tttgaagtac attttgtcat tttgtactcc aaaactgaac tacttacttg catatatacg
    18481 cctttatagt ggagtactac atttacgaga ttcggttaga gtatcagaaa aagaaaaaat
    18541 aaaagttaca gaaatgtata cttcaataaa tggtgaatta tgtaagattg atagagctta
    18601 ttctggagaa attgttattt tgcaaaatga gtttttgaag ttaaatagtg ttcttggaga
    18661 tacaaaacta ttgccacaga gaaaaaagat tgaaaatccg caccctctac tacaaacaac
    18721 tgttgaaccg agtaaacctg aacagagaga aatgttgctt gatgcccttt tggaaatctc
    18781 agatagtgat ccgcttctac gatattacgt ggattctacg acacatgaaa ttatactttc
    18841 tttcttaggg aaagtacaaa tggaagtgat tagtgcactg ttgcaagaaa agtatcatgt
    18901 ggagatagaa ctaaaagagc ctacagtcat ttatatggag agaccgttaa aaaatgcaga
    18961 atataccatt cacatcgaag tgccgccaaa tcctttctgg gcttccattg gtttatctgt
    19021 atcaccgctt ccgttgggaa gtggaatgca gtatgagagc tcggtttctc ttggatactt
    19081 aaatcaatca tttcaaaatg cagttatgga agggatacgc tatggttgcg aacaaggatt
    19141 atatggttgg aatgtgacgg attgtaaaat ctgttttaag tacggtttat actatagccc
    19201 tgttagtact ccagcagatt ttcggatgct tactcctatt gtactggagc aagcctttag
    19261 aaaagctgga acagaattgt tagagccata tcttagtttt aaagtttatg caccacagga
    19321 atatctttca cgggcatata acgatgctcc caaatattgt gcaaatatcg taaatactca
    19381 actgaaaaat aatgaggtca ttattattgg agaaattcct gctcgatgta ttcaagatta
    19441 tcgcaatgat ttaacttttt ttacaaatgg gcttagtgtt tgtttagcag agctaaaagg
    19501 atatcaggtt accactggcg aacctgtttg ccagacccgt cgtctaaata gtcggataga
    19561 taaagtaaga tatatgttca ataaaataac ttagtgcgtt ttatgttgtt atataaatat
    19621 ggtttcttat taaataagat gaaat
//
LOCUS       DARBEC010000292        16345 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.062, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000292 DARBEC010000000
VERSION     DARBEC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16345)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16345)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16345
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.062"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(115..1071)
                     /locus_tag="SCL80_002993"
     CDS             complement(115..1071)
                     /locus_tag="SCL80_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEG1108778.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            1175..1777
                     /locus_tag="SCL80_002994"
     CDS             1175..1777
                     /locus_tag="SCL80_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEG1108779.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            complement(1865..3364)
                     /locus_tag="SCL80_002995"
     CDS             complement(1865..3364)
                     /locus_tag="SCL80_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEG1108780.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(3380..3904)
                     /locus_tag="SCL80_002996"
     CDS             complement(3380..3904)
                     /locus_tag="SCL80_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEG1108781.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            4202..4951
                     /locus_tag="SCL80_002997"
     CDS             4202..4951
                     /locus_tag="SCL80_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEG1108782.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4942..5727
                     /locus_tag="SCL80_002998"
     CDS             4942..5727
                     /locus_tag="SCL80_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEG1108783.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDGGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            5696..6412
                     /locus_tag="SCL80_002999"
     CDS             5696..6412
                     /locus_tag="SCL80_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEG1108784.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     QPLTCYELTKNGRKKLTDYC"
     gene            complement(6514..7548)
                     /locus_tag="SCL80_003000"
     CDS             complement(6514..7548)
                     /locus_tag="SCL80_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEG1108785.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYDIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(7552..8610)
                     /locus_tag="SCL80_003001"
     CDS             complement(7552..8610)
                     /locus_tag="SCL80_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEG1108786.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            complement(8829..9977)
                     /gene="nagA"
                     /locus_tag="SCL80_003002"
     CDS             complement(8829..9977)
                     /gene="nagA"
                     /locus_tag="SCL80_003002"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEG1108787.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(10053..10889)
                     /locus_tag="SCL80_003003"
     CDS             complement(10053..10889)
                     /locus_tag="SCL80_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEG1108788.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(11073..12290)
                     /locus_tag="SCL80_003004"
     CDS             complement(11073..12290)
                     /locus_tag="SCL80_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEG1108789.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(12439..12618)
                     /locus_tag="SCL80_003005"
     CDS             complement(12439..12618)
                     /locus_tag="SCL80_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108790.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            12673..12756
                     /locus_tag="SCL80_003006"
     tRNA            12673..12756
                     /locus_tag="SCL80_003006"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12707..12709,aa:Leu,seq:gag)
     gene            complement(12996..14366)
                     /locus_tag="SCL80_003007"
     CDS             complement(12996..14366)
                     /locus_tag="SCL80_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108791.1"
                     /translation="MNKHNRKITNNKELILLLIGIWGIFLLFNMYMPTMRADDVVYAS
                     RLDELGYLGASIEHYKTWSSRIIIELFLMFFSKHFMLWKLLNSTIMLGIVVLLCKYVF
                     EKINVKNLLLVCSIYCLIPLTVMGETGWRATTLNYQWPVAFSLLTFYPFFQLLRGEEI
                     NRKIYWVSIPLLIFLTNQEQVNACFFVLTSIVSLYLIVNGRYNYKLSVFSIISLAELI
                     FSLTTPGNALRAAHEINKWFPEYKNFNFLNKLDLGISSFGKPFFLDMNILFLLLFFLI
                     FLLTYRKCQNYYVRILTALPFFLNLIIYFGNTMGQSFTYVNGNKRAMIWSSSNLNNLF
                     TELGTKLSLFYPGTWIATLVVLALLLCLIVGIYLSFDNKKTSIFLVILMIMGFCSRLI
                     MGFSPTVWASGMRTYYILYVVIAILVLMAVKELMKSMSVQKNEFMQFGLTVLGICTFI
                     ITVINR"
     gene            complement(15080..15370)
                     /locus_tag="SCL80_003008"
     CDS             complement(15080..15370)
                     /locus_tag="SCL80_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEG1108792.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            complement(15578..16270)
                     /locus_tag="SCL80_003009"
     CDS             complement(15578..16270)
                     /locus_tag="SCL80_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEG1108793.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQALTDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
BASE COUNT         4932 a         3280 c         2740 g         5393 t
ORIGIN      
        1 tcatgtttgt tgaatattgt gacttgtgac cgaggagttg acaggagaac accgtttaaa
       61 gaagaggttg tgaaatcagc tttttgacct gaatattagc tgaattcaca acctctattt
      121 ttggattttt tgttgggaga attctgcctt gatttcaata aaacttttaa aagactgcaa
      181 aaactggaac aattctgctc gatcctcgaa ttcagggcga ctagtcggca gaggcatttg
      241 ccgatacaat acataaactt cttcaatacg tgttaaaatt tgtctgccat catttgactc
      301 tgaaaatgtt tctgcagtat aaatcaataa accatagata tgttttccat aataagctgg
      361 ttgctggatt ctctctagat tttccagcat gtctttaatc acattggcct gtgctcttct
      421 catagagaag taagtttcgt agtatagcgg ctgtctgagc cagtagtttt cttgatgttc
      481 tctggcatct tcttggcttt cgcgaatgaa tgtcagtaaa tgttcgcatt tgatctgcac
      541 atcttcttta ttttctgata aaagaaattc tgccatttgt ctcaaaatat ttcgaaattc
      601 tttttcgatc tgtatttggt tttccttcag ctgcttcgta cgatctggca tgtaaacatt
      661 tgcaagcaaa gcaaatacta atccaatcgc cattaatccc gcttcattgc cgatcagctg
      721 ccaagacatg ttttcctcca ctaaataatg agtgaccaaa accgaattta ctactatccc
      781 ttcggttaat ttgaagcgag aggcaagcgg gatgaacagg aataaaaaaa gactgaagcc
      841 aaaaatagta tacccaacag caaaaaaact gaacagtgct gttgccgtag ctaatataaa
      901 ggctgcgatt cgattcatgc ccgtacgcaa tgtagagcgc ttggtatttc ctacacttaa
      961 taatgcaatg atccctgcag ctggccaata caacagcgat aagctacccg ctgtgaccat
     1021 cgaaaagaag acactgatca ctgttttgac tgttcgcatt cctatacgca tttacttccc
     1081 ccttttttat tttaattata cttgaataat gaaaaactag ctatcagaca aaagtttagg
     1141 tatgatagta aaaagaccaa aaaggagaat ctctatgcaa tttggccgtt ttcgactagg
     1201 tatgcgtacg atcaaaagtg ccttagctgt ttttttatgt atcttgtttt ttcatttgac
     1261 tcaacgagga atcccgatga ttgctgcatt atctgctgtt ttttctttgc gtcaggactt
     1321 gactacaacc gtttcttttg gaaagtctag gatcatcggc aatacattag gtggtacatt
     1381 agctatcatt tatttttttg tcaaaagcta ttttcacaat gattttttgg tagaattgat
     1441 cttgcttcct attctagtta ttattgtgat cgtcgtgtca gatggtatta acaataattc
     1501 cggtatcatt tccgcaatag ctacgttgtt gctgattgcg ttgagcatcc ctcaaggcga
     1561 atccgctttg tatgctatcc aacgtgtact agatactttt atagggacat tcattgcaat
     1621 cggtatcaat ttctttcttc gtcctcctga aacagaaaag aaggaagaac tatcagaaga
     1681 tttggtcgaa ctccaaaaga aagaacgatt gctaagaaca aatttacaag agatagaagc
     1741 taagatccaa aaacaaaaga aaaatagcga ggactaattt tcttttgcaa taaaataaaa
     1801 aaatggagag aagaacttta aaaagtcttc ctccattttt gattgaacgg tgaagtgtac
     1861 tactttattc ttccttcatc gagttttgtt ctgcctcttt tggcgcttca tacatattta
     1921 cttggctagt agtcccacgt gtcgacaaat tctctacgat ttccttcaag tcaagtcctg
     1981 ttgtcgcttt tagtgtttct tgagtagaag aaagcaaatt tgtggcataa tttgtcactc
     2041 ggttcgctcc actggtttct cctgaactgc ttcctgtatc gacaacagag attttttcga
     2101 tatttcctag cggctgtgct gcttcgcgca tcaattgcgg tagcatatcg ataaccatac
     2161 tcaagacagc tgcttcgcca tattccttga aggcattggc gatcttttgt tttgcttcgg
     2221 cttctgcttt tccttttgct agtgctgctt cggcttctgc ttgtccagct aaacgtgtct
     2281 tattggcttc cgcttctgcc agtgcttcca cacggaatcg ttctgcttcg gcttcagtca
     2341 cttctttgac tttttgagct tgtgcttctt gttctttagc gtaccgatcg gcatctgctt
     2401 ttttcttcac ttccgaatca tattgctttt cacgacgtgt gatttctttt tcttccagtt
     2461 cgatttgttt ttggcgttct acgattttaa tctgcatttc ctgttcgatt acttgttgtt
     2521 gtgctcgggc actttctaaa ttgtaggctt ggtctgcttt tgctttagca acatcttgtt
     2581 cttgtttgta ggttgctaat ttcagttctt tttcttttaa tgattctgcg atctctgttt
     2641 gtcgctgtaa ttcagctgct tgtgattctt tttctgcttg agcttttttg atacgcgttt
     2701 cttttagagc ttccgcttct gctatatctg catctctttt gacttgggcg attcttggtt
     2761 tccctaaaga gtcaagataa ccgtttttgt ctctgacctc tttgatagtg aaagaaacga
     2821 tgatcaaccc catttttgca aggtctacag aagcgacttc ttgtacgctt tgactgaact
     2881 tatcgcgatt ttgatagatt tcttctacag tcatagaacc aagaatcgag cgcaagtgtc
     2941 cttctaatac ttctcttgct tcattttcca gttcttctct tgtcttgcct aaaaactgtt
     3001 ctgctgctgt agcaatctct tctacagatg aaccgatttt gatgatcgaa gtaccatcac
     3061 acatcaccgg tacgccttgt tcggtataca cttcaggggt ggagacgtcc aatttactcg
     3121 ataatagact gatccgattc gaacgttgaa aaactggcaa gacgaatgcg ccacctccac
     3181 gcacaatttt gattttgtta ttgctttcat caatgtgcac atttttgctt cctaaataac
     3241 ttccactgat gatcaacgct tcatccggtt ttgctgtctg gtacttcgct acaaagacga
     3301 tcagcaacat cagtaaaatc aaagctgcga ttgcgatcgc gataaaaaca actggtattt
     3361 ccatttaaca cacgctcctt taagctttta tcatgctttc ataaggcatg acataacaga
     3421 ttctttcctt gatctcaatg atcaatactt tcgttccccg ctcagctgtc tgttcttggg
     3481 ggctgtaaaa acaagctgga cgactaatgg aaccggtaac acttttcaac tgaatttctc
     3541 ccatacctcg tacagggatc ggtgtgatca caacagctac ttgtccttcc agagtttttt
     3601 cagaagacga catcgttgat tcggcatgtt tcatcggcat caatatatag aaattaagca
     3661 aaaataccaa cactatcgat atgcctccac tagcaataaa taaaaataaa gatgattgat
     3721 ctgttaaacg ctcacctaga aaaccaaata agcttgtgaa agttaaccaa gggataataa
     3781 gcatcggatc aagcgggcca tcaaaatcga atacatctcc aaaaataatc aataggactg
     3841 ccaatcctgc acaaatcagc aatgtatacc aataaatcgt ctcaagtgcg attcccccta
     3901 tcatatacac gcctccttcc atttctacgt ctatcatacc aatgtttgaa aattctgtat
     3961 agcttctttt accgactcag tggtgttttt tataggaagg ttgtgtttat cgttgacaat
     4021 ttgttaaatt tttgttttca tgttaaaatt ttcacaataa attctttcga ggtagcctat
     4081 gtttcctttt tttaagaaaa aaccattaaa gctccagaac acgatcatcc ttgtcgtttg
     4141 tttctccaca ttcgtttctt tgattgtttc tgccatattg atcaggaatt ttattctttc
     4201 gatgaaccga tctagtgtgt cagataaaat ctctagcatt gctaaaatcg ttgccaatga
     4261 taaactcatc caaaaaaagc tgcagactgg tgacccgaca catgcgattc aaggaataac
     4321 aaatgaaatc atgattgcat cgaatttaga ttttatcgtt gtcatggacc ctgaaggcat
     4381 tcgatattct cacgcagacc caagttacat cggtcttcct ttctcaagtc atgaagatgt
     4441 gaaaaaagtg attggaggac aagaattttt ctctgaaaaa gaaggaatgt tagggttcgg
     4501 cttccgctat ttcgtcccta tttattatga tcaagagatt attggtgcag tttgtgttgg
     4561 cttaacaaac aacacgattg acaatcaagt caaagatgga caaaaaatga ttgttttttc
     4621 attgattatt ggacttagcg taggcatcat aggcgccgtt tttcttgccc aaaaaatgaa
     4681 aaaagtactg cttggacttg aaccacaaga aatagcagaa cagttaagtg aaaaaacgat
     4741 tatcgaaaat gaagtagatg aaggaatctt agccatctct tctgataaaa ctatcctctt
     4801 gatcaataca cctgcaaaaa acattttcta ccaaacagag cagctattgg atttcgaagt
     4861 aggaaaaaaa tcagtgataa tctatatgac gttttgttta aagagacgat tgagcatcaa
     4921 acgaccgttc ggactcaatc catgtattta aatgggatag aaactatttc tagcgttaca
     4981 cctcttttta gcaatgaccg ttttttagga gcagtcgcaa ctttacgaaa tcgatcagaa
     5041 gtaatgaaat taatggagga gcttgggggg acgaaacaat atataaactc catgagagct
     5101 caaacccatg aattcatgaa taaacttcat gtcatttctg gtttgattga tttgaagaag
     5161 tatgaagaag tcacccattt tatccagcag ctaaatcaaa attataaaaa agagattggc
     5221 caagtctcaa gtctgatcaa agtacctgca attgccggtt ttttattagg caaaataaac
     5281 gaagccaaag aacaatctgt tgaaatgctg ttagaaagcg attcttttat ccctaaaatg
     5341 gagatggatg actccatcca ttcacttttg caagtgttag ggaacttact tgataatgca
     5401 aaagaagccg tgctacaaaa acatgaaaga aacggacagg tccggattca tttaaactat
     5461 gacacagaag ctgaagtatt catcgtgacc gtctgcgatg gcggtgtcgg gatatcagaa
     5521 gaaatctcag aaaaaatatt tacgctaggt ttttcgacga agggaatcaa tcgtgggttt
     5581 ggattgaatc tcatccattc aatcgtcaaa cgccacggtg gatttttaga attttccaaa
     5641 caaacaaatg gcggtacaat cgtctatcta gaattaccaa aaaaggaaga tcaaaatgat
     5701 gacaaatgtc ctgataatcg aagatgatcc aatggttgca gaattgaatc gtacttttgt
     5761 ggaaagcatc cctaatatga acgtaatcga aagtttcaga aatgtagaag aaggaaaaaa
     5821 atatttcaga caaaaaaacg cagatctgct gctgcttgat gtatacatgc ctggcgaaag
     5881 tggtatccat tttttatctt ggcttagaca gcaaggaaat gatatcccag tgattttgat
     5941 cactgctgca gatgatatcg aaaccataaa gaatgtgatc cgctacggtg ccttagatta
     6001 tttgatcaaa ccgttcactt tgcaacgttt tcagctggcg atcgaaaagt atcttgcttt
     6061 ctctcagaca atccaaacga ctgaaaaagc cgatcagaaa ttgatcgatc aagtattcaa
     6121 ccacaccact cccattcacg aaataccatc attcatcgat ttgcctaaag gtctgtcaaa
     6181 actgacttta aaaaaaataa gtgcatttat tctaaaagaa gatactattt tttcaacaaa
     6241 agaactggct caaaaaagct cactgtcacg tatatccgtc aagaaatatt tgaatttttt
     6301 agtagaacaa gatatattga acgaaagaat caaatatttg gaaatcggcc agccgctaac
     6361 ctgttatgaa ttgacaaaga atgggcggaa aaagctcaca gattactgtt gagtcatcga
     6421 attatcccat acaaaatagt ccaagtatac agaagtaaca actgtttact tggactattt
     6481 ttaaacattt tattgacgaa tatccatgtt ttcttatctt aattgacggg ctttaggaag
     6541 agatacctca tttttcgttt ttccagtttc tattatttca cgaaagttct cgaaacttgt
     6601 cgaaatcata tttgataatg cctcatctgt atttgaacct acatgcgggg ttacaagaac
     6661 tctagggtac atttctacta attgctggat agcagggtct ggaatcgtct cccatggctt
     6721 gaaggatcgg aaaaaaacct ctgtttcatt tgcaaaaaca tcggttgcaa aaccagctaa
     6781 atgattgctt ttcaaagctc ttaaaatagc ttcattatct tgaagctctc cacgggctgt
     6841 attaatcaag atgctacctt ttttcatttg agctaaaaat ccttcattga tcatttgatc
     6901 gttttcacct gggatatgag gtacatgaat actgacaata tcgcttttgg ctaataactc
     6961 ttcaagcgag tggaacgtca atactttttt agcagcttct gtctgatgaa tatcgtagcc
     7021 gatcactttg gctcctagtc ccttaaacaa aacagcttcc gtcaacccta tcctgcctgt
     7081 tcctatgatc cctactgtgc aatttctgat ttcacgacta aacatttgct catccactcg
     7141 aaaatctttg aaagatgttc ggatcgtcgt atacgcagta tttctcaaaa gcatcatggc
     7201 aaaagtcaaa gataactctg ctatcgcatt tggcgaatac gatggtacac gggctactgt
     7261 cataccaaaa tcagctgccg cttgtagatc gatatgattg actcccacag tccgtgtaaa
     7321 aacataacga atcccatatt cctgcatttt tgcaagattg gtctcatctg ctatacaatt
     7381 tcccctcaac aatacagcat catggtcctt tgctgacacg atattgtcat gtgtcaacag
     7441 ttcttcataa agactgagat cataatggta aatattcaat ttttcaaaaa aatctatctc
     7501 gttcggacga acaccgtaac atgcgattct catactttct tcctccattt cttatgaaaa
     7561 taaacctatg ccatgaatca tttgtaatac aacgatccat acaggtgtca gtaccacagc
     7621 aaaaatagtt gacagtaagg atgcattgga ggctaaaagt gcctccctat caaaactgat
     7681 agcataggct gctgccactg ttgcagctgg tgtagccatc atgataacga ttgttgccag
     7741 tgcttcaaaa ttgactggta gaatattcgt tgccgtcaaa actgctagaa tcacgatatt
     7801 caatgcaggt acaaatacca ctttgactag actgtaatac catgaagttt tttcagatgc
     7861 agcttttttg aagcttactt cacctaatgt tgcgccgatt gctagccacg ccaatggtga
     7921 acttaaacca gctaaataat ccatcggctg aaatagccac ggtgcagtct ggtcaattcg
     7981 caaaaacgct acttggctta tctctccttc tgcacctgta actgacactt gtggcagata
     8041 cccttggaac agccaaatga aaaggccggc aaaagtagcg ataatgattg gattgacgaa
     8101 catcgctttg atatttttag cttccatctt caagccactc atttttatgt atccataaga
     8161 atacaaaaag attcggtagc caatattgaa aattgaggaa tacatcacgc cgacaggacc
     8221 ataaatcgca ctgacgatag gcgttccgaa aaaggtcgta gaaccaaaga tcgtcaggac
     8281 acgcaacgtt gtttgcttat cccctttata gcttaagaaa aaaggttttg aaactagaat
     8341 aagaataata taaataacta atccccaaat caagacattg attccttgca tcagtaattc
     8401 tgtattgata tcttgcatga aagctcgaaa tgccagtgct ggcaaagcca ctgttaagac
     8461 gactttcgat agaatttttc caacagattc agaaaaaatc cccttttttc tacaaacaaa
     8521 ccctaataat ataataaaaa ctgttgaagt gattgcactg acaatcgaca tatctgtcaa
     8581 agtcgatcga aaaatgtcaa ataagttcat ctttctcctc ctaattatgt tcatttattc
     8641 acaataaaaa agcttttatt ttgctaatac gtattaacat ttttaatttt atctttacat
     8701 tgttacttat tgaacaatag gaaattaaag aaacttttga aacttatgta aagtgatttt
     8761 tactagaaca caaaaaaacc ttaaaaccaa acttaagaag tttagtttta aggttaactt
     8821 aaattatatt atgcgtggaa acgttccacg ccatctagat aagttgcttc caatgtcata
     8881 tcagggttca atacgataaa gtctgcatcg cgtccttgag cgatttttcc acatgtatca
     8941 tcgattttac agctgattgc tggaatcaga ctagccatca tgactgcttg ttctggtgtt
     9001 gctaatcccc agtctacaac atttttaatt gcttctttca atttcaatat acttccagct
     9061 aagttgccag attctagacg tgctgttcct tcttttacta ctactggaaa ttcgcctaaa
     9121 ttgtaatttc catcaggcat cccaccggcc atcatacagt ctgtaattaa tgcgacatga
     9181 tcatgtcctg ctttttccat taggattccc gcagcatttg gatgtacatg atggccatca
     9241 caaatcaatt cagaaaagac atgttctaaa gataataacg cacccaccat acctggttcg
     9301 cgatgattca atccacgcat accattgaaa gcatggacaa atacacttgc gcctgcttca
     9361 acagcttttt gcgcttcttc tagtgtagca ttactgtgtc ctagagaaac aactactcct
     9421 tcttcagtca cttgtttgac aaactcttct acaccttgac gttccggtgc taaagcaatt
     9481 tttttgatta aaccacctga agcttcttgc cattcatgga aagtatccaa atcaggatcc
     9541 ccgaaataac ttgggttttg tgcccctttg tgttcttctg tgaagaaagg accttcaaaa
     9601 tagattcctt tgattttagc tcctggtgct tcctgataca ttttgccgat tgtctcagct
     9661 acatctttta aacgttcttt gcttgacgtc aatgttgttg gtaaaaatga agtcactcca
     9721 caagataaca gaccttcaga catcactttg atgccttctg catcattatc cattacatca
     9781 tgattcatat agccgtgaat atgtgtatct accaaaccag gagcaatcca tttgccgctt
     9841 tggtcaatga tgttgacttc tccctcaggt agttctttgg tgtattcacc aaaaagtcca
     9901 tcggtaattt caagatagcc tgcatgtttg acacctgacg ctaaaaagaa tttgtcagca
     9961 tagataaaag ttctcattct attctctccc tttgtttttc tacctctaaa gtactcttat
    10021 ttagagaaga agtcaagaat ggtctacacc atttacaaat tttttgaaat ttgtttcaca
    10081 gcttgtgcta agccttcata gaatgctgct ctttctggtt cttctttacg cttgtatatt
    10141 aagcttttaa gcacaaataa atcattgagg taatagtagc tattcttctc ttgagcccaa
    10201 tagattcctt gattcacctg ttctaacgct tcgtcgattt cattttgatg gaataagaaa
    10261 tttccataat tataaaaaat ttgcgaaaat tcactttttg atcgttcatc agctgattga
    10321 tgcagcagtt ggatgcttcg actcaaatag tagcgtgctt cagccgtttt tcctgccaca
    10381 ccatatattt ttccgataca actaatcagt tgtatttctg tgtcagaaac atggattctt
    10441 tctttttggc tagagtagct taatgcttct ttcaaataat aaagggactg agagatattt
    10501 tgacgcaacg tgaattcgca aacaccgaga taataataat agcgttgaaa gtcagttgct
    10561 tgatacagat tttcagaaat atcagaagat tttagtactt gttctaactt ccgataatca
    10621 cgtttttgga aacaactatc caacagttca aataatttat cgtttgtccg agtagttttt
    10681 cgagagagat tcatcacatg atcaatcgtt acccctagcc gttgacaaat ctgcatcatc
    10741 accaatacat tcggcagttc ctcattattt tcgatccgac ttaatacact ttgtgaacaa
    10801 atatcatgcg aaagcatttt ttgcgtcatt ttttgttctt ttcgaatctc tttaatgatc
    10861 gttccaaagg actcgattaa cgtttccata agtatcctcc ccaaaatatg cgtattcgca
    10921 ttctattcgt attttacttt aggaagtcgc aaacttcaag aaaagacttg caactaaata
    10981 atatacttta gttatttaag aaaactccac tagaaagaga gcctcagtgt gtttgtctgt
    11041 ggccctccta ctgaaaaatt tggtttttaa aatcattttt ggcgataggt tgctaggaat
    11101 tctctcattt tcttagctgt ttgtttcaaa tcatccattt taggaagata aacgattcga
    11161 aaatgatctg gctggttcca attaaatcca ccaccatgta caagtagaat atgatgttca
    11221 tgtaaaaagt ccaaaacgaa tttttcatca tccaaaatat tgaatcgctt tgtatcaatt
    11281 tttgggaata tataaaaagc agcttttggt tttacggctg aaagaccagg aatatcattg
    11341 attgcattat agatatattc tctttgttca tagatccttc cgcctggcag gagcagctca
    11401 tcgacacttt gataaccgcc tagcgcggtt tggataattt gctgagacaa tacattggaa
    11461 cacaaacgca tggaggacaa catattcagc ccttctatat agcctttgac gtgtgactta
    11521 tctccactta ggaccatcca gcctactcga aatcctgcta cacgatgaga tttggataga
    11581 ccgttcagcg taacaacgaa caaatcaggc gctaatgtag caatcggaat atgttctaga
    11641 ccatccatca ctaagcgatc atagatttcg tcggaataaa taatcaggtt attttgtcga
    11701 gctacttcaa cgatttcttc taataattct tttggataaa gtgccccagt aggattatta
    11761 ggattaatga taacaatagc ttttgtccga ctcgttattt ttgatttgat atcatcaata
    11821 tcaggatacc attctgcctc ttcatcgcaa atataatgaa caggtgtgcc tccagctaat
    11881 gcaatagaag ccgtccacaa cggatagtcc ggcattggaa ccagtacttc atctccattg
    11941 tccagcaacc cctgcataca catcgtgatc aattcgctca ctccgttgcc ggtgtagata
    12001 tcattgatcg tcacattcgg aaaatgtttc agttggcaat attgctcaat tgcttttcta
    12061 gcagaaaaga tccccttgga atctgagtag ccttctgagt cacgggcatt catgatcaaa
    12121 tcacgaatga cttcgtttgg tgcttcaaat ccaaaaggtg cgggatttcc cgtgttcaat
    12181 tttaaaatac taatgccctc ttcttgcatc cgctccgctt cttctagaac gggaccgcga
    12241 acatcgtaac tgacaccttc taatttgttg gatttttcaa attctctcat cacttagcca
    12301 atccctcatt tcaaaaagtt agaactttcc aaattttttg ttatttacta tttcaatatc
    12361 atactccatc ctagaaaatt ttgccactta tttaggaaaa acgtaagtat taaaaaaaca
    12421 caaacaagcg ttgagaactt aattgtcatg atagtcattc catagattcg ctgcccaatc
    12481 cattgcaatt ccacaaatga ttcctagcat aaaaccaagc aaaaaacgtc cgaatgcaag
    12541 tccaatcaat acacctaaga tcattcccag tcctgaaaat tctccgtcat atttctccat
    12601 cttaataagt cctcccattt ttgagcttga caactatgta aaacctcgct atcattagtg
    12661 tgttattatt acgcagatgt gttggaattg gcagacaagc atgattgagg gtcatgtgag
    12721 cgttagctcg tgtgggttcg agtcccatca tctgcactac aactcgtgaa gttaattttg
    12781 cttcacgagt ttttttgttt gaacgtttct ttatgccatt atatacatat cactgtatcc
    12841 tttacaaaaa gaacgtacaa tcatgtaaac agcctgaggt gagtatatga aggtcggaaa
    12901 caagctactc gtagaaactt gcaagtgtta aaacaattaa tcgaaaaacg agtaaaatcc
    12961 ctcacctaat actaaagcga gggatttttt gtatattatc tgtttatcac cgttattata
    13021 aaggtacaaa taccaagcac ggttaatcca aattgcataa attcattttt ttgtacactc
    13081 atgcttttca ttagctcttt aactgccatt agtactaaga tggcgataac aacataaagt
    13141 atatagtacg tccgcattcc ggaagcccat actgtgggag aaaatcccat aattaacctt
    13201 gaacaaaatc ccattatcat caaaataact aaaaaaatag aagttttctt attgtcaaaa
    13261 cttaaataaa ttccaacaat taaacatagt aataatgcca agactactaa agtagctatc
    13321 caagtacctg gataaaaaag tgacagtttc gttcctaact ctgtaaagag attattcagg
    13381 ttactactac tccaaatcat tgccctttta tttccattga cgtaagtaaa gctttgaccc
    13441 attgtattac caaaataaat aatcaggttt aagaaaaacg gtaatgcagt caatatacgc
    13501 acataatagt tttgacattt cctatatgtt agaaggaaaa ttaagaaaaa tagcaataaa
    13561 aataagatat tcatgtctaa aaaaaacggc tttccaaatg atgaaatacc taaatctaac
    13621 ttattaagaa agttgaaatt tttatattca ggaaaccatt tattaatttc atgtgcagca
    13681 cgtaatgcat tccctggagt tgtcaaagag aaaattaatt ctgctaagct tattattgaa
    13741 aatactgata acttataatt atatcgtcca ttgacaatca aatataaact aactatactt
    13801 gttagaacaa aaaaacaggc gttgacttgt tcttgattag ttaaaaatat caataaagga
    13861 atacttaccc aatatatttt tctatttatt tcttctcctc ttaagagttg aaaaaacgga
    13921 taaaaagtta gtaaactaaa tgctactggc cactgatagt tcaaggtggt tgctctccaa
    13981 ccggtttccc ccattactgt caatggtatt aaacagtata tagagcaaac caataataaa
    14041 tttttaacat ttattttctc aaaaacatac ttgcaaagta atactacaat tcctaacata
    14101 atcgtagaat ttaataattt ccatagcata aagtgtttag aaaagaacat taagaatagt
    14161 tctataataa ttcttgaact ccatgtctta taatgttcta tcgaagcacc caaatagcca
    14221 agttcatcta atctacttgc ataaactacg tcatccgctc tcatagtcgg catatacata
    14281 ttaaagagta aaaagatacc ccaaatccct attagtaaca agatcagttc tttgttattc
    14341 gttatttttc tattatgttt gttcattttt atactccttc ttatatttcc taatagagta
    14401 taacatagct ttaagcatta cttaaagata acttaaaagt tatcttttaa atacttaatt
    14461 gttaaatacc ttatttgatg caaaagaaat ctacgatagt caacaaaaaa attttcatta
    14521 tatccagaaa aatttttctt agttaaggac ctatggattg cagtaatgca gaattcgtcg
    14581 aataaactgc caaagacata caatcatatt gcttttaaaa ttgacgaaca agatatagat
    14641 tcgtttgtct caaaaatcca aatgttaggt ttaactgtcg aaccaggacg ttctcgtgtg
    14701 aaaggcgagg cttcttcaat ttacttttac gactatgata atcacctctt cgagttacat
    14761 accggtactc tccaggaacg tttaaaatcc tacaattcca ctacctgaga ctagtaatga
    14821 ctagtctctt tttatggcct ctgatccccc cattgagcga atactaatag ggcttcgaat
    14881 tgatctgttc aatctttaag caatatccct caaatgagtt tggaaagttt agtgattcat
    14941 cacaaatact taaatacatg tagttatata tagattgagg ttcttgtgag cgtatgctcg
    15001 tgtgggttca aatcccatcg tctgcatcca tctttccaaa acaagcggct aggacatttt
    15061 tgtctcagcc gcttgttttc tagtttattc tttcatcttg tcggaccttt tttcttgggt
    15121 atagactttt ctcatattct accacaattt tgggtttccc taacacatga taggatctca
    15181 caacaaattg ctttctatta ttataataac ctgtaacgac taacttcatc ccttcgtcta
    15241 cttcagctag gaagttcaaa ctatgggaag caatcaaaca attgacccca tttaaactga
    15301 aacggatcaa cggtctggca ctaacttgca aagtacggat cttatctact gttccatatt
    15361 ttttctccat aaccttcacc tcaataccat tatacgaaca tacattccct ttgtaaagaa
    15421 gaaactctgt tttatctttt tgactttttg tctattacct ttcttaaaat catattggca
    15481 cttcctgcga agatgaagga catgtactaa aaaaaagagg cagcagtata gattactgtt
    15541 gcttctttac taatttaggt ttacttgtta agaaagatta tttacgcaaa tgttcttcaa
    15601 caatttgctg agatttatca gctgtatctt ccacttcatc ttttgctttt tctaactttt
    15661 gaatatattg tttcaagctt gagttttcag cagttgcttc gtctaatttc ttttgtgtat
    15721 ctgttaactg agtttctaca tttgtttttt cttgtgtcag tgttgcgatt tgttgattca
    15781 aaccatttac agtattttgt aattctgaaa gttttgcttg tgcttcttgt agtcttgctg
    15841 tccaatctgc attcagatga ttgatttcat catttttttg gttgatctca tctagtttga
    15901 cattgatttc attttgtttg ctttgtagtt gtgcatcctt gctgactaat tgactttcta
    15961 agttttgttt ttctgctgcc gattgatttt tttggttttc taattcattt acttttgttt
    16021 ggtattcttg gacatctgcc tttgaatctt gcaatgtctg ttctttttgt gctagtcgag
    16081 cggtaagatc tgtaagcgct tgctctttat ctgtcaatgt atgatcaagc tgtgtcagat
    16141 tctcattgat tgtatcaata tctgtctctc caccccacaa aataatttca tttgcgtacc
    16201 gcgtgcctgt gtagccaatt agacatagaa atgcaacgac aatcccacca gtaataaatt
    16261 tctttttcat ttgcatagct ccttaatcca atacttttac atgctaaaag taagtgatct
    16321 caaacacaat gtcaagcgtt tttaa
//
LOCUS       DARBEC010000293        13213 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.079, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000293 DARBEC010000000
VERSION     DARBEC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13213)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13213)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13213
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.079"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(204..2162)
                     /locus_tag="SCL80_003010"
     CDS             complement(204..2162)
                     /locus_tag="SCL80_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEG1108794.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="SCL80_003011"
     CDS             complement(2159..3031)
                     /locus_tag="SCL80_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEG1108795.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="SCL80_003012"
     CDS             complement(3373..4245)
                     /locus_tag="SCL80_003012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEG1108796.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="SCL80_003013"
     CDS             4373..4942
                     /locus_tag="SCL80_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEG1108797.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="SCL80_003014"
     CDS             5009..5746
                     /locus_tag="SCL80_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEG1108798.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="SCL80_003015"
     CDS             5977..7245
                     /locus_tag="SCL80_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEG1108799.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="SCL80_003016"
     CDS             7732..8196
                     /locus_tag="SCL80_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEG1108800.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="SCL80_003017"
     CDS             8180..8809
                     /locus_tag="SCL80_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEG1108801.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="SCL80_003018"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="SCL80_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEG1108802.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="SCL80_003019"
     CDS             10688..11134
                     /locus_tag="SCL80_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEG1108803.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="SCL80_003020"
     CDS             complement(11333..11848)
                     /locus_tag="SCL80_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEG1108804.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="SCL80_003021"
     CDS             11965..13167
                     /locus_tag="SCL80_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEG1108805.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4116 a         2424 c         2708 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaa
//
LOCUS       DARBEC010000294        11243 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.092, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000294 DARBEC010000000
VERSION     DARBEC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.092"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     misc_binding    25..241
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            289..2934
                     /locus_tag="SCL80_003022"
     CDS             289..2934
                     /locus_tag="SCL80_003022"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEG1108806.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            3244..4557
                     /locus_tag="SCL80_003023"
     CDS             3244..4557
                     /locus_tag="SCL80_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEG1108807.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            4547..5200
                     /locus_tag="SCL80_003024"
     CDS             4547..5200
                     /locus_tag="SCL80_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEG1108808.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            5255..5806
                     /gene="radC"
                     /locus_tag="SCL80_003025"
                     /pseudo
     CDS             5255..5806
                     /gene="radC"
                     /locus_tag="SCL80_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
     gene            5937..7022
                     /locus_tag="SCL80_003026"
     CDS             5937..7022
                     /locus_tag="SCL80_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1108809.1"
                     /translation="MKVQRIEVENKPYPLYLLLDKEYQLIEPVMKFIKYLDNTGKSPN
                     TIKAYCYHLKLLYEFMEQRGVILNDINFELLADFVGWLRYPSASNVIDLQSKKAIREE
                     TTVNTILNVVMSFLDYLSRLGEFKSIDVFKQAKGRNFKGFLHHVNKGRYQKNVLKLRV
                     KKKQIRTLRSEEVKQIIDACHTKRDKLILMLMYEGGLRIGEVLSLRLEDIVTWDNQIH
                     LTPRDVNVNEAYIKLRKERTIHVSKELMSLYTDYLIYEYSEELEHDYVFISLKEGYFG
                     KPLKYQSVLDLVRRIVKRTGIEFTSHMLRHTHATQLIREGWDVAFVQKRLGHAHVQTT
                     LNTYVHLSDQDMKNEFNKYLERKEHKK"
     gene            7019..8911
                     /locus_tag="SCL80_003027"
     CDS             7019..8911
                     /locus_tag="SCL80_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001833801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEG1108810.1"
                     /translation="MNASSKRKIISQSEISKKIAVMNEEMQGFWANNSWDIRKCPHPS
                     AIELSKNPALRNRWVRFERVKNLWLRTELKYFYFYHLNNGIWNAKTVWIRKGTVINKM
                     LDFLDLKYPSITSITEVPIEKAMTEYRTYLTKRGVRITTTNYKITANQEKTPVKANSY
                     YVTNLKQFMEFYENFYFDGEEWDKDVWDRRNLPLPDDKVNPTQYEYTINFKGFRNTYF
                     KQLVKRYCKLRLNMDSFSYVSDIAQRLKEFFNFLDMKFKQVQRVHQLTRVEIEAYLSE
                     LNMMGIKPSTITGRISILEGLFSTLLRLEWDDVPSKILIYSEDYPKIPRAKPRFIDEF
                     VLEQLNSHLDKLPEYIATMTMIVQECGMRISELCTLKKGCLLEDKDGDFFLKYYQWKM
                     KKEHIVPISKEVALLIKVREDKVSEEFPDSEYLFPRKDGSPLKQETFRGELNKLAYEQ
                     NIVDKSGEIYRFHAHAFRHTVGTRMINNGMPQHIVQKFLGHESPEMTSRYAHIFDETL
                     KNEFTKFQEKLVTNNGDVLDLDEDNEVDDVELQWFKKNINAQVLPNGYCRLPVVAGGC
                     PHANACLDCTHFCTSKQFLPQHEEQLERTEELLAIAKDKQWQRQVETNSRVKERLEQI
                     IGSLTG"
     gene            8918..9295
                     /locus_tag="SCL80_003028"
     CDS             8918..9295
                     /locus_tag="SCL80_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000361059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEG1108811.1"
                     /translation="MDKQVRNTTEIVRLAKQKSKKTREKVDKAISKFSIEGKVINFNS
                     IAKEANVSKSWLYKEHDIRQRIESLRERQITANVVSKPKKSSRSEEILIKTLKRRVME
                     LEKENKKLQNQIQKLYGDLYNKE"
     gene            9449..10231
                     /locus_tag="SCL80_003029"
     CDS             9449..10231
                     /locus_tag="SCL80_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000067268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(9)-Ia"
                     /protein_id="HEG1108812.1"
                     /translation="MSNLINGKIPNQAIQTLKIVKDLFGSSIVGVYLFGSAVNGGLRI
                     NSDVDVLVVVNHSLPQLTRKKLTERLMTISGKIGNTDSVRPLEVTVINRSEVVPWQYP
                     PKREFIYGEWLRGEFENGQIQEPSYDPDLAIVLAQARKNSISLFGPDSSSILVSVPLT
                     DIRRAIKDSLPELIEGIKGDERNVILTLARMWQTVTTGEITSKDVAAEWAIPLLPKEH
                     VTLLDIARKGYRGECDDKWEGLYSKVKALVKYMKNSIETSLN"
     gene            complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="SCL80_003030"
     CDS             complement(10357..11088)
                     /gene="erm(A)"
                     /locus_tag="SCL80_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001072197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(A)"
                     /protein_id="HEG1108813.1"
                     /translation="MNQKNPKDTQNFITSKKHVKEILNHTNISKQDNVIEIGSGKGHF
                     TKELVKMSRSVTAIEIDGGLCQVTKEAVNPSENIKVIQTDILKFSFPKHINYKIYGNI
                     PYNISTDIVKRITFESQAKYSYLIVEKGFAKRLQNLQRALGLLLMVEMDIKMLKKVPP
                     LYFHPKPSVDSVLIVLERHQPLISKKDYKKYRSFVYKWVNREYRVLFTKNQFRQALKH
                     ANVTNINKLSKEQFLSIFNSYKLFH"
     gene            complement(11146..11205)
                     /locus_tag="SCL80_003031"
                     /pseudo
     CDS             complement(11146..11205)
                     /locus_tag="SCL80_003031"
                     /inference="COORDINATES: protein motif:HMM:NF033690.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="erythromycin resistance leader peptide"
BASE COUNT         3879 a         1718 c         2341 g         3305 t
ORIGIN      
        1 aaaaaaaaaa ccattgtaga atgaagcata acgctgaaca agaggagtaa ctggcacatt
       61 cattcttaga gagagaacta tctgctgaaa ggttctcaat gacaaccagt gaaggtagct
      121 tgggagtttc tgtcttgaat ggagtagaga tagacgagtc gtgatcgtta actcacttga
      181 gaggcaataa tgattgatta ttgcaattta ggtggtaccg cgtaaatcat acgtcctaga
      241 atacgaaagt attttaggac gttttttagt ttaaaggagg aaaacagcat gacggaagaa
      301 aataacctat caacaaaata taatccgcaa gaagttgaag ctggacgtta tcagcattgg
      361 ttagaacaag atttgtttaa accgagcgga gataaaaaag caaaacctta ttcaatcgta
      421 atcccgcccc caaatgttac tggaaaactt catttagggc atgcttggga tacgaccttg
      481 caagatatgc tgattcgtca aaagagaatg caaggatatg atactttatg gctgcctggt
      541 atggaccatg caggaatcgc gacacaagca aaagtagaag cgaagcttag agagcaaggg
      601 atttctcgtt atgatctagg tcgtgaaaag ttcatcgatc aagtgtggga atggaaagaa
      661 gagtatgcga gccacatccg cgaacagtgg gctaaaatgg gcttatcttt agattacagt
      721 agggaacgtt ttacattaga tgatggatta tctgaagcag tacgtaaagt ttttgttacc
      781 ttatacgaaa aaggtttgat ttatcgtggt gaatatatca tcaactggga tcctcaagca
      841 cgaacagctc tatctgatat cgaagttatt cataaagata tcgaaggcgc cttttatcat
      901 atgagctatg aactagcaga tggatcaggc gtagtagaaa ttgcgacaac ccgtccagaa
      961 acgatgttgg gagatacggc gattgctgtt catccagaag acgagcgtta tcaagcgctc
     1021 atcggcaaaa aagtgattct acctttagtc aataaacaaa tcccaatcat tgcagatgaa
     1081 tatgtagata tggaattcgg gacaggtgta gtaaaaatca ctccagcaca tgatccaaac
     1141 gactttgagg taggtaatcg tcatgatctt ccacgcgtaa atgttatgga tgatgatggt
     1201 acgatgaatg aactagcagg aaaatatgct ggaatggaac gttttgctgc tcgtaaagca
     1261 atcgtgcaag atctgaaaga aatcggtcga ttgattaaaa tcgatacgat gatccacagt
     1321 gtgggtcatt ccgagcggac aggtgtcgtt gttgaacctc ggctgtctac tcaatggttt
     1381 gtaaaaatgg cgccgttagc gaaaaaagcc attgataacc aagaaacaga tcaagcagtg
     1441 gaattcttcc ctccaagatt caatcaaacg tttttacgtt ggatggaaaa tgtccatgat
     1501 tgggtaatct cacgacaatt atggtgggga catcaaattc ctgcatggta tcacaaagaa
     1561 acaggtgaaa tgtatgtagg gatggaagca ccagcggata gtgaaaactg ggtacaagat
     1621 cctgatgttt tagacacttg gtttagttca gctttgtggc ctttctcaac aatgggctgg
     1681 ccagatacgg aaagcgaaga ttataagcga tacttcccta ctagcacttt agtaaccggt
     1741 tatgacatca ttttcttctg ggtaagcaga atgattttcc aaagcttgga atttactggc
     1801 gaacgtccat ttgagaatgt cttgattcat ggattgattc gtgacgaaca agggcggaag
     1861 atgagtaaat cattaggtaa tggaatcgat ccgatggaag taatcgaaaa gtatggagca
     1921 gatgctctcc gctggttctt atcgaatggt tctgcaccag gacaagatgt ccgcttcagc
     1981 tatgaaaaaa tggatgcttc ttggaacttt atcaacaaaa tctggaatgc ttcacgtttc
     2041 gttatcatga atatcgaagg aatgaccgta gaagagatcg atttatctgg agaaaaatca
     2101 gtggctgacc ggtggatcct tactcgtttg aatgaaacga tcgaacgagt aactgagcta
     2161 tttgatcgtt ttgaatttgg tgaagcaggt cgacaactgt ataactttat ctgggatgat
     2221 ttttgtgact ggtacatcga aatgagtaaa gaagtacttt atggagatga tgctgtttca
     2281 aaacaaacca cccgtagcat cttagtccat acacttgatc agatcctacg tttattgcat
     2341 ccaatcatgc cattcgttac agaagaaata tgggaacata ttcctcatca gggaaattct
     2401 ctagtagtgg cagaatatcc tgttgtacgt ccagagtttg atgatgaaac agcttctaaa
     2461 gggatggaag tattgaaaga attgattcga gccgttcgaa atattcgttc agaagtgaat
     2521 acaccattgt ctaaaccaat tacgttgcta attaaaacaa acgatgctaa aatcgatcaa
     2581 tttttagttg aaaatacaag ctatatcgaa cgcttctgta atcccgaaga gttgacgata
     2641 tcaagtgaga tcactgcgcc agatctagcg atgtcagctg ttctgacagg tgcagaaatc
     2701 ttcttgccgt tagcaggatt gatcaatatt gaagaagaaa tcaaacgatt agaaaaagaa
     2761 ctggaaaagt ggacaagcga agtaaaacgt gtccaaggaa aactttctaa tgaacgtttt
     2821 gtttctaacg cgccggaaga agtagtagag gcagagcgtg caaaagaaaa agattattta
     2881 gagaagcaaa cggctgtcaa agaacgaatc gaaagtttgc gtactattca ataagaatag
     2941 aaaaagagag tgtgaaatca gctagctatt actaaaagct agctgattga acatcgttac
     3001 aggaaagagt ttgagcctca ctcttagctt cgattcataa ggagatgtga cataaatttt
     3061 gtcgcatctc ttttttagtc tactcttatt atgtaggaga aggagggagg tggatcatgt
     3121 atcaagagtc aataaagtag ttgataaaat cgttcctctc tctgtttctt ttctctttgt
     3181 catactttta tttttcttat gtgttctatt tgttataata aaagagaaat gggggaattg
     3241 ttgatgacga ttgaagaagc aatagaatgg atccatagtc gattaccatt tggttcacgt
     3301 cctggccttg accgagtcga agctttatta gaagtggtag ggcatccaga gagaaaagta
     3361 aaaacgatcc atatcgctgg aacaaacgga aaaggttcta ctgtagctta tttgagaagc
     3421 ctattagaag aggctgggct gacagtaggt acatttactt caccttatat cgaaacgttc
     3481 aatgaacgga tcgcaatcaa tggcaaaagc attccagaca atgatttgat ccagcttgta
     3541 aaaaaatatc agccactgat caaacaactt gatcaatttg aagcagtagc tggtattact
     3601 gaattcgaga cattaacagc aatggcattt gattattttt tgatgaagaa agtggacgta
     3661 gcgattatcg aagttggctt aggcggctta ttggattcta ccaatgtagc tgtaccaatg
     3721 ctgacgggaa taacaacgat tggacttgat cataccgaaa tacttggaga tacaattgaa
     3781 gaaatcgctg cacaaaaagc tggaatcatc aaacaaggcg ttccagtagt aacaggaaat
     3841 attgccccag aagcttttac tgtcatccaa caaacagcta aagcaaaaaa tgcaccgctt
     3901 tatgtatacg gggaaaatta taaaataaac tatctgcatc ctgatgagat gtggggagaa
     3961 gtatttgaat tttttaatga aataggaaag atccctcaat taaccactcc gcttttaggg
     4021 caacatcaag ctgaaaatgc aggagtagcc atcgaattgt attttgttta ctgtcaattg
     4081 gaaaagctgc ctttccgaga aaaagatgtt ttgaagggat tgaagaaatc tcgatggcca
     4141 ggaagaatgg agcgtttgag taacgagcca ttagttgttt tagatggtgc ccataatacg
     4201 catgcaatga accggcttgt tgagaatttg aaaaaagaat tcaaaggata tcaaatcaac
     4261 attttgtttt ccgccttacg aactaaaaat gttagagaaa tgctagaacg attgcagcaa
     4321 gtaccaaatg tccatattta tttgacgacc tttgaacatc caaaagccat cgaattaagt
     4381 gaagtatcag atttagcaag tgagagagta tcaatcgttt cattgtggca attcggatta
     4441 gcagatatat tggaaaagat gacaaatgaa gacgtattag ttgtgaccgg ttcactttat
     4501 ttcatttcag aagttcgcca tttattaata caattaggag gagatcatga agtataatgc
     4561 agtgattttt gacatggatg gtttgctgtt cgacacagag ttagtgtatt acgaagccac
     4621 acaagtagta gcagacaaga tgggttttcc ttatgataaa gacctgtatt taaaattctt
     4681 aggtgtttca gatgaagaag tatgggcgaa ttatcatatg attttctcgg aatttggcaa
     4741 aacaagtgta caaagattca tagatgattc ataccaggaa acgcttgaac gtttttcgca
     4801 aggagcggta caaatgaagc ccggtgtgtt ggaactcctc tcttttttag aagaacatca
     4861 gattcccaaa gttgtcgctt ccagtaacca acgagcagtc attgaacttt tattgaagaa
     4921 gaacaatctg tttgaacgat ttgagacgat tgtttctgca gaaaatgttc aacgagcaaa
     4981 accagaccca gaaattttct tgttagctaa tcagaagcta ggtacagcga agaaagaaac
     5041 attgatttta gaagattcac aaaatggtat tttagcagct tctgcggctg agatcccggt
     5101 agtcatggta ccagatctgc ttccaccatc ccaagaattg atcaaaaaaa cagctggggt
     5161 tgtttcttct ttacatgaaa tccctgcatt gttaaaataa aaatccatct cttttcgtat
     5221 cttattttag ataagataga aaggagatgt ttttttggga aaacgattga tcaaagaagt
     5281 acctactagc tctttaccaa gggaacgtat ggaaatttat ggcgcggaag ctttatcgga
     5341 tcaggagctt ttggcaatct tactgcgtac cggtcaacac ccttatagtg tgatgtcgat
     5401 tgctggaaat ctgttaaaaa cgtttggagg actggcctct ttacgacagg ctactttgca
     5461 tgagttagaa gagattcgag ggataggacg cgtgaaagca atcgaaatca aagccttgat
     5521 cgaattaggt cgacgaattc agacggatca tgaaagagcg gctccaccgg ttcgtaccag
     5581 ctacgaattg gcccagcaat tgattctgga aatgaaagat cacaaacaag aacatttggt
     5641 ttgtatttat ttggacacga agaatcaagt gatcttaaaa aaaacagtgt tcatcggttc
     5701 gctgaatcag tcagtcgctc atcctagaga aattttccat tatgctgtac gttattgtgc
     5761 agcaaggatc gttttagcgc ataatcatcc ttctggtaat gttttaacca tagtataata
     5821 aataatagta gttaaagtag tttaagagga gatattaaag ggatatttta ctttaaataa
     5881 aaattaacaa ggtttataat ttgtagtagt taaagtattt aaagaggtgg gaacatgtga
     5941 aggttcaaag gatagaagtg gagaataagc cgtatccatt gtatttatta ctagataaag
     6001 aataccagct aatagaacca gtaatgaaat ttattaaata cttagataat actggtaagt
     6061 ctcctaatac cattaaagca tactgctatc atttaaagtt gctgtacgag ttcatggaac
     6121 agagaggtgt tattcttaat gatattaact ttgagttgtt agcagacttc gtaggttggt
     6181 tgagatatcc ttcagcatca aatgtaattg atcttcagtc aaaaaaagcc ataagagaag
     6241 aaacgacagt gaatacaatt ttaaatgtag ttatgagttt tcttgattat ttaagtagat
     6301 taggagaatt taaatcaatt gatgtattta aacaagcaaa gggaagaaat ttcaaaggat
     6361 ttttacatca tgttaataag ggtagatacc aaaagaatgt cttaaagtta agggttaaaa
     6421 agaaacagat aagaacattg agatcagagg aagttaagca aattattgat gcttgtcata
     6481 cgaaaagaga taaattaatt ttaatgctta tgtatgaggg tggtttaaga atcggtgaag
     6541 tgttatcgct taggcttgaa gatattgtca cttgggacaa tcaaatccat ttaacaccta
     6601 gagatgttaa tgttaatgaa gcttatatta aattaaggaa ggaaagaaca atacatgtga
     6661 gtaaagaact tatgtcactt tatacagatt acttgatata tgagtatagt gaggaattgg
     6721 agcatgatta tgtttttatt tccttaaaag aaggctattt tgggaaacca ctaaagtacc
     6781 aaagtgttct tgatctagtt agaagaatag ttaaaaggac tggaatagaa tttacatcac
     6841 atatgcttcg ccacactcac gcaacgcagc taattaggga aggatgggat gttgcgttcg
     6901 ttcaaaagag attaggtcac gcacatgttc agacaacgtt aaatacctat gttcatcttt
     6961 cagatcagga tatgaaaaat gagtttaata aatacctcga gagaaaggag cataagaaat
     7021 gaatgcttct agtaaaagga aaattattag tcagagtgag attagcaaaa aaatagctgt
     7081 aatgaatgaa gaaatgcagg ggttttgggc taataatagt tgggatataa gaaaatgtcc
     7141 acatccttct gccatagaat taagtaagaa tcctgcttta aggaatcgtt gggttcgttt
     7201 tgaacgtgtt aaaaatctgt ggttaagaac agaattgaaa tatttttatt tttaccattt
     7261 aaacaatgga atatggaatg caaaaactgt ctggattaga aaaggaacag taattaataa
     7321 aatgttagat ttcttagatt taaagtatcc tagcattact tcaattactg aagttcctat
     7381 tgaaaaagca atgacggagt atagaactta tttgacaaaa cggggtgtta gaattaccac
     7441 tactaattat aagattactg ctaatcaaga aaaaacacct gtaaaagcta attcctacta
     7501 tgttactaat ctaaaacaat ttatggagtt ttatgagaac ttttattttg atggagagga
     7561 gtgggataaa gacgtttggg atagacgtaa cttacctttg ccagatgata aggttaaccc
     7621 aacacaatat gaatatacaa ttaactttaa agggtttcgg aatacatatt ttaaacaact
     7681 tgtaaaaaga tattgtaagt tgagattgaa catggatagc ttttcctatg taagtgatat
     7741 tgcccaaaga cttaaagagt tctttaattt tctggacatg aaatttaaac aagttcagag
     7801 agtacaccaa ttaacgagag tggaaattga agcatattta agtgaactaa acatgatggg
     7861 aataaaacct agtacaataa ctgggaggat ctctatattg gaaggactat ttagtaccct
     7921 tcttaggcta gaatgggatg atgttccttc caaaatatta atttattctg aggactatcc
     7981 gaaaatacca agagcaaaac cacgctttat agatgaattc gtcctagagc aattgaacag
     8041 tcatcttgat aaattacccg aatatatagc tacgatgact atgattgttc aagaatgtgg
     8101 aatgaggata agtgaattgt gcaccttgaa aaaaggctgt ctattagagg acaaagatgg
     8161 agatttcttt ttaaagtatt atcaatggaa aatgaaaaag gagcatatag ttccaatatc
     8221 taaagaggta gctttactta ttaaagttcg ggaagataaa gtttcagagg aatttccaga
     8281 tagtgaatac ctctttccaa gaaaagatgg atcgccatta aaacaagaaa catttagagg
     8341 tgagttaaat aaattagctt atgagcaaaa tatagtggat aaatcaggtg agatttatag
     8401 attccatgcc catgcctttc gccatacagt aggaacaaga atgattaaca acgggatgcc
     8461 ccagcatatt gtgcagaaat ttttggggca tgaaagccca gaaatgacaa gcagatacgc
     8521 tcatatcttt gatgaaactc taaaaaatga atttactaaa tttcaggaaa aactggttac
     8581 caataatgga gatgtgcttg atctagatga agataatgaa gtcgatgatg tagagcttca
     8641 atggttcaag aaaaatataa atgcacaagt gcttccaaat ggttattgta gattgccagt
     8701 agtagcaggt ggttgtccac atgcgaatgc atgcttagat tgcactcact tctgtaccag
     8761 taagcaattc ttaccacagc acgaagaaca gttagagcgt acagaagagt tattagccat
     8821 agctaaggat aaacaatggc aaagacaagt agagactaat agccgtgtta aagagcgttt
     8881 agaacaaatc attggaagtt tgacggggta attatcaatg gataaacaag ttagaaatac
     8941 aacagaaatt gtacgtttgg cgaagcagaa atcaaaaaag acaagggaaa aagtagacaa
     9001 agcgatttct aaattttcga ttgaaggtaa agttattaat tttaattcaa tagcaaagga
     9061 agctaatgtt tctaaatcat ggctttataa ggaacacgat attaggcaaa gaatcgaatc
     9121 ccttcgtgag cgtcaaataa cagcaaatgt agtctcaaaa cccaagaaaa gttctcgttc
     9181 ggaggaaatc cttattaaaa ccttaaaaag aagagtaatg gaattagaaa aagaaaataa
     9241 aaaattacag aaccaaattc aaaaattata tggagatctg tataataaag aataattatt
     9301 aatctgtaga caaattgtga aaggatgtac ttaaacgcta acggtcagct ttattgaaca
     9361 gtcatttagg tatatgtcca atctagggta agtaaattga gtatcaatat aaactttata
     9421 tgaacataat cagttacgag gtgaaatcat gagcaatttg attaacggaa aaataccaaa
     9481 tcaagcgatt caaacattaa aaatcgtaaa agatttattt ggaagttcaa tagttggagt
     9541 atatctattt ggttcagcag taaatggtgg tttacgcatt aacagcgatg tagatgttct
     9601 agtcgtcgtg aatcatagtt tacctcaatt aactcgaaaa aaactaacag aaagactaat
     9661 gactatatca ggaaagattg gaaatacgga ttctgttaga ccacttgaag ttacggttat
     9721 aaataggagt gaagttgtcc cttggcaata tcctccaaaa agagaattta tatacggtga
     9781 gtggctcagg ggtgaatttg agaatggaca aattcaggaa ccaagctatg atcctgattt
     9841 ggctattgtt ttagcacaag caagaaagaa tagtatttct ctatttggtc ctgattcttc
     9901 aagtatactt gtctccgtac ctttgacaga tattcgaaga gcaattaagg attctttgcc
     9961 agaactaatt gaggggataa aaggtgatga gcgtaatgta attttaaccc tagctcgaat
    10021 gtggcaaaca gtgactactg gtgaaattac ctcgaaagat gtcgctgcag aatgggctat
    10081 acctctttta cctaaagagc atgtaacttt actggatata gctagaaaag gctatcgggg
    10141 agagtgtgat gataagtggg aaggactata ttcaaaggtg aaagcactcg ttaagtatat
    10201 gaaaaattct atagaaactt ctctcaatta ggctaatttt attgcaataa caggtgctta
    10261 cttttaaaac tactgattta ttgataaata ttgaacaatt tttgggaaga ataaagcgtc
    10321 ctcttgtgaa attagagaac gctttattac tttaatttag tgaaacaatt tgtaactatt
    10381 gaaaatagaa agaaattgtt ccttcgatag tttattaata ttagtgacat ttgcatgctt
    10441 caaagcctgt cggaattggt ttttagtgaa aagaacacga tattcacggt ttacccactt
    10501 ataaacaaaa gatcgatact ttttgtagtc cttctttgaa atcaatggtt gatgtcgttc
    10561 aagaacaatc aatacagagt ctacacttgg cttaggatga aaatatagtg gtggtacttt
    10621 tttgagcatt tttatatcca tctccaccat taatagtaaa cccaaagctc gttgcagatt
    10681 ttgcaatctt ttcgcaaatc ccttctcaac gataagatag ctatatttag cctgactttc
    10741 aaaggtaatt cttttgacaa tatccgtact gatgttataa ggaatattac catatatctt
    10801 atagtttata tgttttggga aggaaaattt tagaatatcc gtttgaatca cttttatatt
    10861 ctcagagggg tttaccgctt ctttagtcac ttgacataag cctccatcaa tttctatagc
    10921 agtaactgat cgactcattt tgactagctc tttggtaaaa tgtccttttc ctgatccgat
    10981 ttctattacg ttgtcttgtt tactgatatt cgtgtgattc aatatttctt ttacatgctt
    11041 tttagaagta ataaaatttt gcgtgtcttt agggtttttc tggttcatta taaccttctc
    11101 cttactggtt ataatgaact gacattaagc gttcattata accaattaat tttgatttgg
    11161 ttgataacga actttattaa taacaaatat agaaaaagta cccatacttt tatctccttt
    11221 ccattttttt cattatgaat gag
//
LOCUS       DARBEC010000295         8066 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.114, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000295 DARBEC010000000
VERSION     DARBEC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.114"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(134..577)
                     /locus_tag="SCL80_003032"
     CDS             complement(134..577)
                     /locus_tag="SCL80_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEG1108814.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="SCL80_003033"
     CDS             complement(795..2135)
                     /locus_tag="SCL80_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEG1108815.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="SCL80_003034"
     CDS             2404..3300
                     /locus_tag="SCL80_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEG1108816.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="SCL80_003035"
     CDS             complement(3450..4391)
                     /locus_tag="SCL80_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEG1108817.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="SCL80_003036"
     CDS             4517..5638
                     /locus_tag="SCL80_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEG1108818.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="SCL80_003037"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="SCL80_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEG1108819.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="SCL80_003038"
     CDS             7426..7992
                     /locus_tag="SCL80_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEG1108820.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
BASE COUNT         2672 a         1505 c         1598 g         2291 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatg
//
LOCUS       DARBEC010000296         6450 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.122, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000296 DARBEC010000000
VERSION     DARBEC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6450
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.122"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            142..2589
                     /locus_tag="SCL80_003039"
     CDS             142..2589
                     /locus_tag="SCL80_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEG1108821.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            2819..3886
                     /gene="rlmN"
                     /locus_tag="SCL80_003040"
     CDS             2819..3886
                     /gene="rlmN"
                     /locus_tag="SCL80_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEG1108822.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(4079..5182)
                     /locus_tag="SCL80_003041"
     CDS             complement(4079..5182)
                     /locus_tag="SCL80_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEG1108823.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            5735..6283
                     /gene="aac(6')"
                     /locus_tag="SCL80_003042"
     CDS             5735..6283
                     /gene="aac(6')"
                     /locus_tag="SCL80_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEG1108824.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         2157 a         1103 c         1381 g         1809 t
ORIGIN      
        1 ttcttgataa aatcatttag aaattaagaa aaactgtaaa ataagctgaa aaatgaaaga
       61 aagcgtcagt tctttcaaac aaaactttag ttcccttact tttttttgta taatgaaact
      121 tgatgaaatg agggataata aatggcacaa aagaaacggc ctactaaaaa aaggaaaaag
      181 acgaaaaaaa cgaaaaagca aaaacagcag caggaacaaa tgctgacgat tggtgttggg
      241 tttgccttga ttttatttgc tgtcttcggc tttttaaaat taggtttttt aggaatattg
      301 attgcaaacg gctttcggat cattgcagga aatacttacc aaatcctctg tcttttatta
      361 gccgtattag gattttggat tgtcattaag aatacagagt tttcaattgg taaaaaccga
      421 cgctggtttg gcggcatact cttttatttc ggtatcttat tattgttaca tgcccattta
      481 tttggcaaac tccatactgg ggaaccaaat atcatgggga cgacatggga tttgttagcc
      541 agtgatataa aacaaagtca agtagacaat aacgttggtg gtgggatgat tggagcaatc
      601 ctttatcatt ttacttactt cttgattgca cagcctggaa gttatttagc agcagtgcta
      661 ttactggcag gaggagcatt cctgttcagt aatctggaag gctatcagct gttaaatggt
      721 atccaatcaa ttggagaacg agtacaggaa ttattggaag gtgatcctgc caagcaagct
      781 cgaaagcaag cggcaaagga agagagaatg aaacagcgcg cagaagcgaa agaagcaagg
      841 cgcttagctg caaaagaagc tgcagaaaaa gaagctgcag aatatgagaa gaaaaaagcg
      901 gtgagccagc aaagggaaaa tactgctgat gaatggcagg aaccagaaaa tacagagccg
      961 gagcagctaa gttttgttcc aatcgacagt tttcaagaaa atatccatcc tgcgaatcta
     1021 gagaaaccag taccagatac accaaaacag accaatacag cagaaggttt ttcagatgag
     1081 ttgccagagg atgacggtac ctcattagaa tttgagattg aagcagaaca ggaaaatcaa
     1141 gactatgaat taccgacagt tgatttgctt gattctatac caactgtgga tcaaagtgat
     1201 gagtataaaa aaattgaaaa aaatatcggc gtattagaac aaactttcca aagtttcggt
     1261 gtcgatgcca aggtcgtcaa agccagctta ggaccatccg tcacaaaatt cgaagtccag
     1321 ccggctgtag gggtaaaagt aagtaaaatt gtgaacttaa cagatgatat cgcgcttgct
     1381 ttagcggcga aagatgtccg gatggaagca ccgattccag gtaaatcact gattggtatt
     1441 gaagtaccaa atggaaagat cagtatggtt tcctttagag aaatcatcga agcacaacca
     1501 aaccacccag ataaattact agaagtacct ttaggacgtg atgtttctgg acgggtacaa
     1561 acggctgatc tttcgaagat gcctcactta ctcgtggctg gttcgactgg tagtgggaag
     1621 tcagtagcaa ttaatgggat tatcacaagt atattaatgc gtgctaagcc ccatgaagta
     1681 aaactgatga tgatcgatcc gaaaatggtt gaattgaata tgtacaacgg catacctcat
     1741 ctgttgacgc cggtcgtgac gaaccctaga aaagcagcgc aagccttaca aaaagtagta
     1801 caagaaatgg aagaacgata tgaaaaattc gcggcaaccg gagtaagaaa tatttctggt
     1861 tacaatgaat ttgttcaaca gaaaaacttg gaaaacggaa cgaaacatcc aaccttacca
     1921 ttcatcgtag tgatcgtaga tgaattagct gatttgatga tggtagccag caatgaagtt
     1981 gaagatgcga ttattcgttt agcgcaaatg gctcgggcag cggggattca tatgatccta
     2041 gctactcaac gtccaagtgt ggatgttatc acaggaatca tcaaagcaaa tgtaccttcc
     2101 agaatggcat ttgcggtatc aagcggtaca gattctcgga cgatcattga ttctaatggg
     2161 gcagaaaagc tcttagggcg aggagatatg ctattcttgc caatgggtga gaataaaccg
     2221 atccgtgtgc aaggagcatt catttctgac cacgaagtag aacgggtcgt tcaatttgtt
     2281 acagatcagc aagaagctca ttatgaagag aaaatgatgc cgacagatga agttgaaacg
     2341 acaggtgctc cagaacagcc acaagatgag ctgtttgaag aagcaaaagc acttgtcgtt
     2401 gaaatgcaaa cggcaagtat ttcccttctc caaagaagat ttcggatcgg ttacaaccga
     2461 gcagcacgtt tagtagatga actggaagct catggagttg ttggcccgtc agaaggaagc
     2521 aagcctcgga aagtattcat tgagcaggaa gaaacatcag aaacgttgcc ggacgataat
     2581 gacatgtaaa acttgtgtga tgaagggttg aaaaacgaat aaccaataaa aagacaaggc
     2641 atgatgatac caaaagaaaa aatggttttt ttccatcttt tctaatacgg tatgatgtct
     2701 tgtcttttta gtttttgtga gattaaagtt ctattcgatt tatttgtgaa gtattgcatt
     2761 aaggaaatga atcatgtata gtatgtagcg aaactttgag aaaaagaggg aaaatacttt
     2821 ggaaaaacag tccatttatg gcttgacgaa tgaagaatta atcaattggt ttatagaaaa
     2881 cggcgagaaa aaattccgag ctgcccaagt ttgggaatgg ttgtatcaaa aacgtgtaag
     2941 taatttcaca gaaatgacga atctatcgaa acaattgatc gaaaaattat cggagcattt
     3001 tatcatcaat cctctaaaac agatggtcgt ccaagaagct tcggatggta cagtaaaata
     3061 tttgtttgaa ctccctgata aaaacatgat tgagactgta ctgatgagac aagaatatgg
     3121 cttgtcagtt tgtgtgacga cacaagtagg atgtaatatc ggctgtacgt tttgtgcaag
     3181 cgggctgctc aaaaagaacc gtgatctgac tgctggagaa attgttgcac agatcatgat
     3241 ggtccaacat tattttgatg agcgcaatct tggcgaacgc gtttcacatg tcgttgtaat
     3301 ggggatcggt gagccatttg ataattacga caatgtaatg gactttttgc atatcatcaa
     3361 tgatgccaaa ggattggcaa ttggtgctcg gcacattaca gtttccacta gtggattagc
     3421 acacaaaatc aaggaattcg ctaataacgg cttgcaagtc aatctggcta tttccttgca
     3481 cgcgccgaat aatgaggtgc gaacatcgat catgcggatc aaccgaagtt tcccaattga
     3541 gaaattgatg gaagctgtgg atgagtattt agagaaaaca aaccgtcgaa tcacgtttga
     3601 atatatcatg ttgaatcaag taaatgatag accagagcat gcgcaacaat tagcagatct
     3661 attaaaagat aagaaaaaat tagcatatgt caacttgatt ccatataatc cagtcagtga
     3721 acatgatcaa tattctcgca gtccaaaagc tgatgtattg agattttatg atgtattgaa
     3781 gaaaaacggc gtaaactgtg tgatacgtaa agaacatggt acagatattg atgcagcttg
     3841 cggacaattg cgaagcaagc aaatgaaaaa aacagttgcc aaataatcgt atcctactcg
     3901 ttttttcaat taccataaaa ggagcagttt tcttaattaa ggaactgttc aacttaaata
     3961 agagaaaaga ttctatagcg aaagctataa agcttttttt gacaagaaaa accctcatca
     4021 agcaaagaaa tgtcctcaat ttgatgacag cctgcttgat gggggtttta gcaattattt
     4081 aactgatttt cttatatcct tgctggcttt cttgtgtagc atgataagtt tctacgtcaa
     4141 gaatggcctg tttaaatttt tctcgggtga tttcataagg cacatgttta atatcatttg
     4201 ttgccatggc tttatctaag attgcttcta aatcggtcgt gacattgaga ttcatgtcgg
     4261 ttaacgatac tggaagttgg ttctttttca taaacacata gattttatca aacaaatcga
     4321 actgctgatc catcatcaat aaggtcagta caccataact gacagatgct ccgtgaaggt
     4381 atccctccat tttttctaga acagtcgtac cgtaatagaa tgagtgtgcc atagcagaat
     4441 tatagttatt gttaactaaa acagaaacca atccggtagt aacaatgata tccgaaacga
     4501 cttgttcaat tgcatatgag ggacgatcgt cggcactatc tttcaatgct tgcgtaccat
     4561 aagtcaagag acgatcaatg cacatttttc cgatttggat tcctaatcta tccgtgtagc
     4621 ctagatcatc gccacgagca gaaaaagtag attcgacttc tttggataaa gcatcgccaa
     4681 tacctgccca taagtattta tcaggtgctt cggcaataat tgctgtatca atgaaggtgt
     4741 gtacaggtgg ttttgaagga aagaagagtc cgtccattac gccttcttcg ttatagatga
     4801 cacataccgc agtgacgggc gaacagttcg aaccaatagt gggaaaggtg aagactggtt
     4861 tagctagttt ttcaccaatg acttttacag tatcgcaagc acggccacca ccgactgcaa
     4921 acaggacgtc tgctttttgg acactttcat gctggatcaa ctgttcaata tttccataag
     4981 ttgaattacc cccgtaccag agactggcga caacctctat ttcctgtgtc aaagctactt
     5041 ttattttgtt ttctgccttt gaaagtgcag tcttccctcc aatcagcact acttttgtcc
     5101 catattcttt gacgattcca tttgcttttt catacgcatc tgttccaatg gtataatttg
     5161 gtaagaaaac actttgactc atattcattt ccttctttct tcgttgaaac gggttaaaat
     5221 tttaaagagt gatcgatctg actgcggctg tagatggagt agaggtgaac ctaagacagg
     5281 ataagtgatg aataaggtgg catgaacacc ttgaaatcat caataagtat cctaaaaacg
     5341 aacaagtaca aaaacattga aaacgacaat aatgaatttt tgttcctctt agtatttcta
     5401 ccgattccct ttgctttttt gtaattatct aaaaattcta aaaattaaga ttattatatg
     5461 aaaacaggtt ttatttgtca attcataatt ggtttttttt aaagcaaaag ataatctata
     5521 tcatgatgaa caaacagagt tttttcctcc aattgatggc tttcctgatc tttcctattt
     5581 tgaataaagt gtttctgtat aattttttta ttttataagt aagataaata atgaaagaat
     5641 agagatactg attggtagct aaagattttt gagcgagaac atttcgtttt ttcaaaagga
     5701 ctgttagaat aagcaagaac aagggggaag acgtatgata atcagtgaat ttgaccgtaa
     5761 taatccagta ttgaaagatc agctttctga tttactgaga ctgacttggc cggaagaata
     5821 tggagacagc tcggcagaag aagtagaaga aatgatgaat ccagaacgaa tcgcggtagc
     5881 agcggtagac caagatgagt tagtaggatt tattggtgca atccctcaat acggtatcac
     5941 aggttgggaa ttgcatccat tagttgtaga aagctcccga cgaaagaacc aaataggtac
     6001 tcgattagtc aattacttag aaaaagaagt agcttccaga ggaggaatca cgatttattt
     6061 aggtacggat gatttagacc atggaacaac gttaagtcaa acggacctgt atgagcatac
     6121 atttgataaa gtggcttcca tccagaacct tcgtgaacat ccgtatgaat tctatgaaaa
     6181 attaggttat aaaatcgtag gtgtcttacc aaatgcaaat ggctgggaca aaccggatat
     6241 ttggatggca aaaacgatta ttcctcgacc aaattctcaa taataaaaag aggttgtgac
     6301 agaagtaatc agcttcaaga aataagaagg aatttacgaa aattacttct tgaatttttg
     6361 tgaaattcca acttatttcc gaagaagcta cttttggaac accgtgtacc agaggttgtg
     6421 aaaaagtcgc ttagtttcga gtagtaagaa
//
LOCUS       DARBEC010000297         5487 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.129, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000297 DARBEC010000000
VERSION     DARBEC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5487
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.129"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            complement(40..1026)
                     /locus_tag="SCL80_003043"
                     /pseudo
     CDS             complement(40..1026)
                     /locus_tag="SCL80_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032495458.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS1542 family transposase"
     gene            1178..1873
                     /gene="vanR"
                     /locus_tag="SCL80_003044"
     CDS             1178..1873
                     /gene="vanR"
                     /locus_tag="SCL80_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEG1108825.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            1851..3005
                     /locus_tag="SCL80_003045"
     CDS             1851..3005
                     /locus_tag="SCL80_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEG1108826.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
     gene            3220..4188
                     /locus_tag="SCL80_003046"
     CDS             3220..4188
                     /locus_tag="SCL80_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEG1108827.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            4181..5212
                     /gene="vanA"
                     /locus_tag="SCL80_003047"
     CDS             4181..5212
                     /gene="vanA"
                     /locus_tag="SCL80_003047"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEG1108828.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALVVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            5218..5448
                     /locus_tag="SCL80_003048"
                     /pseudo
     CDS             5218..5448
                     /locus_tag="SCL80_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX"
BASE COUNT         1654 a         1065 c         1234 g         1534 t
ORIGIN      
        1 ggattttatt ctttatttaa aactttgcaa cagaacccta tttccgataa ccgtgtattg
       61 aaaggcatct tcgaagccat tatccaatat ttcgcaggct tttgtatatt taggctggtc
      121 gatatattca ccgactaagg cattcttagc tgttcgagcg agttcaatat ccgtaaactt
      181 aaagatcgct tttactgctt ctctaaacgg ttttgaattc ttttttggaa tggaactgaa
      241 gatgtttctt aaaaaatgga cctggcatct ctgccaactt gcgttggtaa atgacttacg
      301 aatcgcagac actaggcctt tatgggcatc agaaatgatg agttctgtcc cctttaggcc
      361 gcgttctttt aagtattcaa agaagatgga ccatgtgtca tcactttctt cattttgaat
      421 catgaagcca atgatttcac ggtcgccacc ttctgttatc ccgatcgcaa tatggcagct
      481 tttagaaagc actcgatggt cctcacggac ttttatgtag agaacatcag tcatcagata
      541 aggataattc gtacctgaga gtgaacggtt ctgccattcg ttgaccatcg ggtctaactg
      601 ctcagtcagg ctagaaacaa aagatttcga tacagatttt ccacatagct cttcaacaat
      661 ctttgaaact ttacgtgtcg aaacgcccga aacatacatc tcaagcattg aagcgagcag
      721 tgccttttca tttcgctgat aacgctcaaa caccgtcggt gaaaattcac catcacgtgt
      781 tctaggcact tttaattcga gtgtacccac acgagtcgta aagtctcgct cataatagcc
      841 atttctttga ctctgacgac tttctgaacg ttcatagtca tcggcttgaa tatattctgt
      901 tcgttgattt tccatcaatt ggttgaaaac agtggttaaa atatttttag aaacatcatc
      961 ttttaccgaa tgttcaataa tactttgaac ctcttcgttg ttcagtgtaa aatgtacttg
     1021 ggtcatgtaa cgtcctcctg ggtatgtttt tgtggttaaa aacattgtac cgtaaaaggg
     1081 ctgttacatg gccttttatc ttttacacaa ttatatggac tttatcctat aatctgataa
     1141 attattaagg gcaaacttat gtgaaagggt gataactatg agcgataaaa tacttattgt
     1201 ggatgatgaa catgaaattg ccgatttggt tgaattatac ttaaaaaacg agaattatac
     1261 ggttttcaaa tactataccg ccaaagaagc attggaatgt atagacaagt ctgagattga
     1321 ccttgccata ttggacatca tgcttcccgg cacaagcggc cttactatct gtcaaaaaat
     1381 aagggacaag cacacctatc cgattatcat gctgaccggg aaagatacag aggtagataa
     1441 aattacaggg ttaacaatcg gcgcggatga ttatataacg aagccctttc gcccactgga
     1501 gttaattgct cgggtaaagg cccagttgcg ccgatacaaa aaattcagtg gagtaaagga
     1561 gcagaacgaa aatgttatcg tccactccgg ccttgtcatt aatgttaaca cccatgagtg
     1621 ttatctgaac gagaagcagt tatcccttac tcccaccgag ttttcaatac tgcgaatcct
     1681 ctgtgaaaac aaggggaatg tggttagctc cgagctgcta tttcatgaga tatggggcga
     1741 cgaatatttc agcaagagca acaacaccat caccgtgcat atccggcatt tgcgcgaaaa
     1801 aatgaacgac accattgata atccgaaata tataaaaacg gtatgggggg ttggttataa
     1861 aattgaaaaa taaaaaaaac gactattcca aactagaacg aaaactttac atgtatatcg
     1921 ttgcaattgt tgtggtagca attgtattcg tgttgtatat tcgttcaatg atccgaggga
     1981 aacttgggga ttggatctta agtattttgg aaaacaaata tgacttaaat cacctggacg
     2041 cgatgaaatt atatcaatat tccatacgga acaatataga tatctttatt tatgtggcga
     2101 ttgtcattag tattcttatt ctatgtcgcg tcatgctttc aaaattcgca aaatactttg
     2161 acgagataaa taccggcatt gatgtactta ttcagaacga agataaacaa attgagcttt
     2221 ctgcggaaat ggatgttatg gaacaaaagc tcaacacatt aaaacggact ctggaaaagc
     2281 gagagcagga tgcaaagctg gccgaacaaa gaaaaaatga cgttgttatg tacttggcgc
     2341 acgatattaa aacgcccctt acatccatta tcggttattt gagcctgctt gacgaggctc
     2401 cagacatgcc ggtagatcaa aaggcaaagt atgtgcatat cacgttggac aaagcgtatc
     2461 gactcgaaca gctaatcgac gagttttttg agattacacg gtataaccta caaacgataa
     2521 cgctaacaaa aacgcacata gacctatact atatgctggt gcagatgacc gatgaatttt
     2581 atcctcagct ttccgcacat ggaaaacagg cggttattca cgcccccgag gatctgaccg
     2641 tgtccggcga ccctgataaa ctcgcgagag tctttaacaa cattttgaaa aacgccgctg
     2701 catacagtga ggataacagc atcattgaca ttaccgcggg cctctccggg gatgtggtgt
     2761 caatcgaatt caagaacact ggaagcatcc caaaagataa gctagctgcc atatttgaaa
     2821 agttctatag gctggacaat gctcgttctt ccgatacggg tggcgcggga cttggattgg
     2881 cgattgcaaa agaaattatt gttcagcatg gagggcagat ttacgcggaa agcaatgata
     2941 actatacgac gtttagggta gagcttccag cgatgccaga cttggttgat aaaaggaggt
     3001 cctaagagat gtatataatt ttttaggaaa atctcaaggt tatctttact ttttcttagg
     3061 aaattaacaa tttaatatta agaaacggct cgttcttaca cggtagactt aataccgtaa
     3121 gaacgagccg ttttcgttct tcagagaaag atttgacaag attaccattg gcatccccgt
     3181 tttatttggt gcctttcaca gaaagggttg gtcttaatta tgaataacat cggcattact
     3241 gtttatggat gtgagcagga tgaggcagat gcattccatg ctctttcgcc tcgctttggc
     3301 gttatggcaa cgataattaa cgccaacgtg tcggaatcca acgccaaatc cgcgcctttc
     3361 aatcaatgta tcagtgtggg acataaatca gagatttccg cctctattct tcttgcgctg
     3421 aagagagccg gtgtgaaata tatttctacc cgaagcatcg gctgcaatca tatagataca
     3481 actgctgcta agagaatggg catcactgtc gacaatgtgg cgtactcgcc ggatagcgtt
     3541 gccgattata ctatgatgct aattcttatg gcagtacgca acgtaaaatc gattgtgcgc
     3601 tctgtggaaa aacatgattt caggttggac agcgaccgtg gcaaggtact cagcgacatg
     3661 acagttggtg tggtgggaac gggccagata ggcaaagcgg ttattgagcg gctgcgagga
     3721 tttggatgta aagtgttggc ttatagtcgc agccgaagta tagaggtaaa ctatgtaccg
     3781 tttgatgagt tgctgcaaaa tagcgatatc gttacgcttc atgtgccgct caatacggat
     3841 acgcactata ttatcagcca cgaacaaata cagagaatga agcaaggagc atttcttatc
     3901 aatactgggc gcggtccact tgtagatacc tatgagttgg ttaaagcatt agaaaacggg
     3961 aaactgggcg gtgccgcatt ggatgtattg gaaggagagg aagagttttt ctactctgat
     4021 tgcacccaaa aaccaattga taatcaattt ttacttaaac ttcaaagaat gcctaacgtg
     4081 ataatcacac cgcatacggc ctattatacc gagcaagcgt tgcgtgatac cgttgaaaaa
     4141 accattaaaa actgtttgga ttttgaaagg agacaggagc atgaatagaa taaaagttgc
     4201 aatactgttt gggggttgct cagaggagca tgacgtatcg gtaaaatctg caatagagat
     4261 agccgctaac attaataaag aaaaatacga gccgttatac attggaatta cgaaatctgg
     4321 tgtatggaaa atgtgcgaaa aaccttgcgc ggaatgggaa aacgacaatt gctattcagc
     4381 tgtactctcg ccggataaaa aaatgcacgg attacttgtt aaaaagaacc atgaatatga
     4441 aatcaaccat gttgatgtag cattttcagc tttgcatggc aagtcaggtg aagatggatc
     4501 catacaaggt ctgtttgaat tgtccggtat cccttttgta ggctgcgata ttcaaagctc
     4561 agcaatttgt atggacaaat cgttgacata catcgttgcg aaaaatgctg ggatagctac
     4621 tcccgccttt tgggttatta ataaagatga taggccggtg gcagctacgt ttacctatcc
     4681 tgtttttgtt aagccggcgc gttcaggctc atccttcggt gtgaaaaaag tcaatagcgc
     4741 ggacgaattg gactacgcaa ttgaatcggc aagacaatat gacagcaaaa tcttaattga
     4801 gcaggctgtt tcgggctgtg aggtcggttg tgcggtattg ggaaacagtg ccgcgttagt
     4861 tgttggcgag gtggaccaaa tcaggctgca gtacggaatc tttcgtattc atcaggaagt
     4921 cgagccggaa aaaggctctg aaaacgcagt tataaccgtt cccgcagacc tttcagcaga
     4981 ggagcgagga cggatacagg aaacggcaaa aaaaatatat aaagcgctcg gctgtagagg
     5041 tctagcccgt gtggatatgt ttttacaaga taacggccgc attgtactga acgaagtcaa
     5101 tactctgccc ggtttcacgt catacagtcg ttatccccgt atgatggccg ctgcaggtat
     5161 tgcacttccc gaactgattg accgcttgat cgtattagcg ttaaaggggt gataagcatg
     5221 gaaataggat ttactttttt agatgaaata gtacacggtg ttcgttggga cgctaaatat
     5281 gccacttggg ataatttcac cggaaaaccg gttgacggtt atgaagtaaa tcgcattgta
     5341 gggacatacg agttggctga atcgcttttg aaggcaaaag aactggctgc tacccaaggg
     5401 tacggattgc ttctatggga cggttaccgt cctaagcgtg ctgtaaacgg ttctgttgca
     5461 aagttttaaa taaagaataa aatccct
//
LOCUS       DARBEC010000298         1781 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.194, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000298 DARBEC010000000
VERSION     DARBEC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1781
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.194"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            24..416
                     /locus_tag="SCL80_003049"
     CDS             24..416
                     /locus_tag="SCL80_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000393259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEG1108829.1"
                     /translation="MKLLFIDDNMVFDIDNLKGFLNDTSSFGFIAKENNKIIGFAYCY
                     TLLRPDGKTMFYLHSIGMLPNYQDKGYGSKLLSFIKEYSKEIGCSEMFLITDKGNPRA
                     CHVYEKLGGKNDYKDEIVYVYDYEKGDK"
     gene            417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="SCL80_003050"
     CDS             417..>1781
                     /gene="aph(2'')-I"
                     /locus_tag="SCL80_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012655884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APH(2'')-Ia/If/Ih family aminoglycoside
                     O-phosphotransferase"
                     /protein_id="HEG1108830.1"
                     /translation="MNIVENEICIRTLIDDDFPLMLKWLTDERVLEFYDGRDKKYTLE
                     SLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYHYPKTDEIVYGMDQFI
                     GEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAIRAYQKSGFRIIEDLP
                     EHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVDSIEIIGSGYDSVAYL
                     VNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKE
                     IKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYIL
                     LRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGII
                     DFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPI
                     ETIVY"
BASE COUNT          731 a          136 c          303 g          611 t
ORIGIN      
        1 tatagttgaa gaatattatc ctattgaaac tattgtttat tgatgataat atggtttttg
       61 atattgataa tttaaaagga tttcttaatg ataccagttc atttgggttt atagctaaag
      121 aaaataataa aattatagga tttgcatatt gctatacact tttaagacct gatggaaaaa
      181 caatgtttta tttacactca ataggaatgt tacctaacta tcaagacaaa ggttatggtt
      241 caaaattatt atcttttatt aaggaatatt ctaaagagat tggttgttct gaaatgtttt
      301 taataactga taaaggtaat cctagagctt gccatgtata tgaaaaatta ggtggtaaaa
      361 atgattataa agatgaaata gtatatgtat atgattatga aaaaggtgat aaataaatga
      421 atatagttga aaatgaaata tgtataagaa ctttaataga tgatgatttt cctttgatgt
      481 taaaatggtt aactgatgaa agagtattag aattttatga tggtagagat aaaaaatata
      541 cattagaatc attaaaaaaa cattatacag agccttggga agatgaagtt tttagagtaa
      601 ttattgaata taacaatgtt cctattggat atggacaaat atataaaatg tatgatgagt
      661 tatatactga ttatcattat ccaaaaactg atgagatagt ctatggtatg gatcaattta
      721 taggagagcc aaattattgg agtaaaggaa ttggtacaag atatattaaa ttgatttttg
      781 aatttttgaa aaaagaaaga aatgctaatg cagttatttt agaccctcat aaaaataatc
      841 caagagcaat aagggcatac caaaaatctg gttttagaat tattgaagat ttgccagaac
      901 atgaattaca cgagggcaaa aaagaagatt gttatttaat ggaatataga tatgatgata
      961 atgccacaaa tgttaaggca atgaaatatt taattgagca ttactttgat aatttcaaag
     1021 tagatagtat tgaaataatc ggtagtggtt atgatagtgt ggcatattta gttaataatg
     1081 aatacatttt taaaacaaaa tttagtacta ataagaaaaa aggttatgca aaagaaaaag
     1141 caatatataa ttttttaaat acaaatttag aaactaatgt aaaaattcct aatattgaat
     1201 attcgtatat tagtgatgaa ttatctatac taggttataa agaaattaaa ggaacttttt
     1261 taacaccaga aatttattct actatgtcag aagaagaaca aaatttgtta aaacgagata
     1321 ttgccagttt tttaagacaa atgcacggtt tagattatac agatattagt gaatgtacta
     1381 ttgataataa acaaaatgta ttagaagagt atatattgtt gcgtgaaact atttataatg
     1441 atttaactga tatagaaaaa gattatatag aaagttttat ggaaagacta aatgcaacaa
     1501 cagtttttga gggtaaaaag tgtttatgcc ataatgattt tagttgtaat catctattgt
     1561 tagatggcaa taatagatta actggaataa ttgattttgg agattctgga attatagatg
     1621 aatattgtga ttttatatac ttacttgaag atagtgaaga agaaatagga acaaattttg
     1681 gagaagatat attaagaatg tatggaaata tagatattga gaaagcaaaa gaatatcaag
     1741 atatagttga agaatattat cctattgaaa ctattgttta t
//
LOCUS       DARBEC010000299         1504 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.203, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000299 DARBEC010000000
VERSION     DARBEC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1504)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.203"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            262..345
                     /locus_tag="SCL80_003051"
                     /pseudo
     CDS             262..345
                     /locus_tag="SCL80_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527320.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
     gene            394..477
                     /locus_tag="SCL80_003052"
     CDS             394..477
                     /locus_tag="SCL80_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuator leader
                     peptide ErmL"
                     /protein_id="HEG1108831.1"
                     /translation="MLVFQMRYVDKTSTVLKQTKNSDYADK"
     gene            602..1339
                     /gene="erm(B)"
                     /locus_tag="SCL80_003053"
     CDS             602..1339
                     /gene="erm(B)"
                     /locus_tag="SCL80_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEG1108832.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESRASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
BASE COUNT          551 a          224 c          245 g          484 t
ORIGIN      
        1 atttgtatcg ataagaaata gatttaaaaa tttcgctgtt attttgtaca tttaacttga
       61 cggtgacatc tctctattgt gagttattag tggtacagtt ttcaaccgtt ttaattataa
      121 aaaagtggtg catttttaaa ttggcacaaa caggtaacgg ttattgcagg tgtatttctt
      181 atctatgggt ttaacatgga ttttatcatt aaaatcatga gtattgtccg agagtgattg
      241 gtcttgcgta tggttaaccc taaagttatg gaaataagac ttagaagcaa acttaagagt
      301 gtgttgatag tgcattatct taaaattttg tataatagga attgaagtta aattagatgc
      361 taaaaatttg taattaagaa ggagggattc gtcatgttgg tattccaaat gcgttatgta
      421 gataaaacat ctactgtttt gaaacagact aaaaacagtg attacgcaga taaataaata
      481 cgttagatta attcctacca gtgactaatc ttatgacttt ttaaacagat aactaaaatt
      541 acaaacaaat cgtttaactt ctgtatttgt ttatagatgt aatcacttca ggagtgataa
      601 catgaacaaa aatataaaat attctcaaaa ctttttaacg agtgaaaaag tactcaacca
      661 aataataaaa caattgaatt taaaagaaac cgataccgtt tacgaaattg gaacaggtaa
      721 agggcattta acgacgaaac tggctaaaat aagtaaacag gtaacgtcta ttgaattaga
      781 cagtcatcta ttcaacttat cgtcagaaaa attaaaactg aatactcgtg tcactttaat
      841 tcaccaagat attctacagt ttcaattccc taacaaacag aggtataaaa ttgttgggaa
      901 tattccttac catttaagca cacaaattat taaaaaagtg gtttttgaaa gccgtgcgtc
      961 tgacatctat ctgattgttg aagaaggatt ctacaagcgt accttggata ttcaccgaac
     1021 actagggttg ctcttgcaca ctcaagtctc gattcagcaa ttgcttaagc tgccagcgga
     1081 atgctttcat cctaaaccaa aagtaaacag tgtcttaata aaacttaccc gccataccac
     1141 agatgttcca gataaatatt ggaagctata tacgtacttt gtttcaaaat gggtcaatcg
     1201 agaatatcgt caactgttta ctaaaaatca gtttcatcaa gcaatgaaac acgccaaagt
     1261 aaacaattta agtaccgtta cttatgagca agtattgtct atttttaata gttatctatt
     1321 atttaacggg aggaaataat tctatgagtc gcttttgtaa atttggaaag ttacacgtta
     1381 ctaaagggaa tgtagataaa ttattaggta tagggcacct ctaataacta ccaattaatt
     1441 cacctcaaaa tgaaattaga aaaaaacagc gaaattttta aatctatttc ttatcgatac
     1501 aaat
//
LOCUS       DARBEC010000300         1382 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.212, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000300 DARBEC010000000
VERSION     DARBEC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.212"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            <1..1311
                     /locus_tag="SCL80_003054"
     CDS             <1..1311
                     /locus_tag="SCL80_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aminoglycoside N-acetyltransferase
                     AAC(6')-Ie/aminoglycoside O-phosphotransferase
                     APH(2'')-Ia"
                     /protein_id="HEG1108833.1"
                     /translation="ESLKKHYTEPWEDEVFRVIIEYNNVPIGYGQIYKMYDELYTDYH
                     YPKTDEIVYGMDQFIGEPNYWSKGIGTRYIKLIFEFLKKERNANAVILDPHKNNPRAI
                     RAYQKSGFRIIEDLPEHELHEGKKEDCYLMEYRYDDNATNVKAMKYLIEHYFDNFKVD
                     SIEIIGSGYDSVAYLVNNEYIFKTKFSTNKKKGYAKEKAIYNFLNTNLETNVKIPNIE
                     YSYISDELSILGYKEIKGTFLTPEIYSTMSEEEQNLLKRDIASFLRQMHGLDYTDISE
                     CTIDNKQNVLEEYILLRETIYNDLTDIEKDYIESFMERLNATTVFEGKKCLCHNDFSC
                     NHLLLDGNNRLTGIIDFGDSGIIDEYCDFIYLLEDSEEEIGTNFGEDILRMYGNIDIE
                     KAKEYQDIVEEYYPIETIVYGIKNIKQEFIENGRKEIYKRTYKD"
BASE COUNT          584 a          108 c          234 g          456 t
ORIGIN      
        1 gaatcattaa aaaaacatta tacagagcct tgggaagatg aagtttttag agtaattatt
       61 gaatataaca atgttcctat tggatatgga caaatatata aaatgtatga tgagttatat
      121 actgattatc attatccaaa aactgatgag atagtctatg gtatggatca atttatagga
      181 gagccaaatt attggagtaa aggaattggt acaagatata ttaaattgat ttttgaattt
      241 ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa taatccaaga
      301 gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc agaacatgaa
      361 ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga tgataatgcc
      421 acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt caaagtagat
      481 agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa taatgaatac
      541 atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga aaaagcaata
      601 tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat tgaatattcg
      661 tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac ttttttaaca
      721 ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg agatattgcc
      781 agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg tactattgat
      841 aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta taatgattta
      901 actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc aacaacagtt
      961 tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct attgttagat
     1021 ggcaataata gattaactgg aataattgat tttggagatt ctggaattat agatgaatat
     1081 tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa ttttggagaa
     1141 gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata tcaagatata
     1201 gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat taaacaggaa
     1261 tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg attatataat
     1321 atatgaaaag ctattataaa agacggttct gttgcaaagt tttaaatcta ctatcaaata
     1381 ag
//
LOCUS       DARBEC010000301         1297 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.219, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000301 DARBEC010000000
VERSION     DARBEC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1297)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1297)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1297
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.219"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            46..297
                     /locus_tag="SCL80_003055"
                     /pseudo
     CDS             46..297
                     /locus_tag="SCL80_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            390..1184
                     /locus_tag="SCL80_003056"
                     /note="aph(3')-IIIa"
     CDS             390..1184
                     /locus_tag="SCL80_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEG1108834.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
BASE COUNT          412 a          223 c          308 g          354 t
ORIGIN      
        1 cattttcctc taggaaaaaa ttaactagca ccacgggtta tcttttatat agaagatatc
       61 gccgtatgta aggatttcag ggggcaaggc ataggcagcg cgcttatcaa tatatctata
      121 gaatgggcaa agcataaaaa cttgcatgga ctaatgcttg aaacccagga caataacctt
      181 atagcttgta aattctatca taattgtggt ttcaaaatcg gctccgtcga tactatgtta
      241 tacgccaact ttgaaaacaa ctttgaaaaa gctgttttct ggtatttaag gttttagaat
      301 gcaaggaaca gtgaattgga gttcgtcttg ttataattag cttcttgggg tatctttaaa
      361 tactgtagaa aagaggaagg aaataataaa tggctaaaat gagaatatca ccggaattga
      421 aaaaactgat cgaaaaatac cgctgcgtaa aagatacgga aggaatgtct cctgctaagg
      481 tatataagct ggtgggagaa aatgaaaacc tatatttaaa aatgacggac agccggtata
      541 aagggaccac ctatgatgtg gaacgggaaa aggacatgat gctatggctg gaaggaaagc
      601 tgcctgttcc aaaggtcctg cactttgaac ggcatgatgg ctggagcaat ctgctcatga
      661 gtgaggccga tggcgtcctt tgctcggaag agtatgaaga tgaacaaagc cctgaaaaga
      721 ttatcgagct gtatgcggag tgcatcaggc tctttcactc catcgacata tcggattgtc
      781 cctatacgaa tagcttagac agccgcttag ccgaattgga ttacttactg aataacgatc
      841 tggccgatgt ggattgcgaa aactgggaag aagacactcc atttaaagat ccgcgcgagc
      901 tgtatgattt tttaaagacg gaaaagcccg aagaggaact tgtcttttcc cacggcgacc
      961 tgggagacag caacatcttt gtgaaagatg gcaaagtaag tggctttatt gatcttggga
     1021 gaagcggcag ggcggacaag tggtatgaca ttgccttctg cgtccggtcg atcagggagg
     1081 atatcgggga agaacagtat gtcgagctat tttttgactt actggggatc aagcctgatt
     1141 gggagaaaat aaaatattat attttactgg atgaattgtt ttagtaccta gatttagatg
     1201 tctaaaaagc tttaactaca agctttttag acatctaatc ttttctgaag tacatccgca
     1261 acggttctgt tgcaaagttt taaatctact atcaaat
//
LOCUS       DARBEC010000302         1195 bp    DNA     linear   BCT 07-NOV-2023
DEFINITION  TPA_asm: Enterococcus faecium isolate VRE4383
            SAMEA114569944-rid20112363.guided.225, whole genome shotgun
            sequence.
ACCESSION   DARBEC010000302 DARBEC010000000
VERSION     DARBEC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA114569944
            Sequence Read Archive: ERR12203769
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (07-NOV-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 07-NOV-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001972638.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001972638.1
            Genome Coverage       :: 369x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/07/2023 13:47:59
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,057
            CDSs (total)                      :: 2,999
            Genes (coding)                    :: 2,932
            CDSs (with protein)               :: 2,932
            Genes (RNA)                       :: 58
            rRNAs                             :: 1, 3 (16S, 23S)
            partial rRNAs                     :: 1, 3 (16S, 23S)
            tRNAs                             :: 50
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 67
            CDSs (without protein)            :: 67
            Pseudo Genes (ambiguous residues) :: 0 of 67
            Pseudo Genes (frameshifted)       :: 23 of 67
            Pseudo Genes (incomplete)         :: 33 of 67
            Pseudo Genes (internal stop)      :: 17 of 67
            Pseudo Genes (multiple problems)  :: 5 of 67
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA114569944-rid20112363.guided.225"
                     /isolate="VRE4383"
                     /db_xref="taxon:1352"
                     /country="Denmark"
     gene            260..>1195
                     /gene="vanY"
                     /locus_tag="SCL80_003057"
     CDS             260..>1195
                     /gene="vanY"
                     /locus_tag="SCL80_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanY-A/VanY-F/VanY-M family D-Ala-D-Ala
                     carboxypeptidase"
                     /protein_id="HEG1108835.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNWFCCKVLNLLSN"
BASE COUNT          426 a          174 c          238 g          357 t
ORIGIN      
        1 ccttatttga tagtagattt aaaactttgc aacagaaccc ccattgtcaa caggcagttc
       61 agcctcgtta aattcagcat gggtatcact tatgaaaatt catctacatt ggtgataata
      121 gtaaatccag tagggcgaaa taattgactg taatttacgg ggcaaaacgg cacaatctca
      181 aacgagattg tgccgtttaa ggggaagatt ctagaaatat ttcatacttc caactatata
      241 gttaaggagg agactgaaaa tgaagaagtt gtttttttta ttgttattgt tattcttaat
      301 atacttaggt tatgactacg ttaatgaagc actgttttct caggaaaaag tcgaatttca
      361 aaattatgat caaaatccca aagaacattt agaaaatagt gggacttctg aaaataccca
      421 agagaaaaca attacagaag aacaggttta tcaaggaaat ctgctattaa tcaatagtaa
      481 atatcctgtt cgccaagaaa gtgtgaagtc agatatcgtg aatttatcta aacatgacga
      541 attaataaat ggatacgggt tgcttgatag taatatttat atgtcaaaag aaatagcaca
      601 aaaattttca gagatggtca atgatgctgt aaagggtggc gttagtcatt ttattattaa
      661 tagtggctat cgagactttg atgagcaaag tgtgctttac caagaaatgg gggctgagta
      721 tgccttacca gcaggttata gtgagcataa ttcaggttta tcactagatg taggatcaag
      781 cttgacgaaa atggaacgag cccctgaagg aaagtggata gaagaaaatg cttggaaata
      841 cgggttcatt ttacgttatc cagaggacaa aacagagtta acaggaattc aatatgaacc
      901 atggcatatt cgctatgttg gtttaccaca tagtgcgatt atgaaagaaa agaatttcgt
      961 tctcgaggaa tatatggatt acctaaaaga agaaaaaacc atttctgtta gtgtaaatgg
     1021 ggaaaaatat gagatctttt attatcctgt tactaaaaat accaccattc atgtgccgac
     1081 taatcttcgt tatgagatat caggaaacaa tatagacggt gtaattgtga cagtgtttcc
     1141 cggatcaaca catactaatt ggttctgttg caaagtttta aatctactat caaat
//
